title: Comprehensive study of hormone-independent highly productive strain of Rauvolfia serpentina tissue culture as a source of indole alkaloids
abstract: Aim.To characterize a new hormone-independent strain К-27М of R. serpentina tissue culture.Methods.Plant tissue culture, biochemical and cytological analysis, statistical methods.Results.The strain is maintained on the specially designed simple-composition hormone-free 10C medium.The maximum biomass yield was at day 69 of subculture (693.5 g/l of live biomass, 43.0 g/l of dry biomass), the weight of cell biomass increased 15-18 times over a subculture period.The maximum content of indole alkaloi
journal: Biopolymers and Cell