title: Useful Molecular and Cytogenetic Approaches in Population Genetics Studies of Pine Species
abstract: In the last decade, we characterised Portuguese populations of Pinus sylvestris L., Pinus nigra Arnold and Pinus pinaster Aiton by using different molecular and cytogenetic approaches. DNA markers helped assess intra- and inter-population genetic variability, extrapolation of phylogenies, provenances and/or infraspecific taxonomy. Quantitative real-time polymerase chain reaction (qRT-PCR), Classical Cytogenetics techniques and/or Comet assay were valuable to assign origins, infraspecific taxa or
journal: Genetics.