title: Development and verification of real-time PCR assay for identification of viral agents causing acute respiratory infections in human beings
abstract: A multiplex polymerase chain reaction (PCR) for identification of four viruses causing acute respiratory diseases in human beings was developed. The analytical sensitivity of developed RT-PCR for identification of adenovirus, respiratory-syncytial virus, flu viruses types A and B, and actual subtypes of type A flu virus (seasonal and pandemic variants H1N1, seasonal H3N2, and viruses of bird flu that are pathogenic to human beings H5 and H7) was 1 × 10<sup>3</sup> genome equivalents per millilit
journal: Molecular Genetics Microbiology and Virology