title: IMPROVED METHOD OF PURIFICATION OF Plum pox virus AND SEROLOGICAL ANALYSIS OF THE COAT PROTEIN
abstract: Molecular weight heterogeneity of the coat protein (CP) is commonly observed in the purified potyvirus preparations due to partial proteolysis of the N-terminal domain exposed on the surface of viral particles. The cellular proteases released from disrupted plant tissues are believed to attribute to the partial CP proteolysis during purification and storage of the virus. The N-terminal domain is the most variable part of the CP. It displays virus-and strain-specific epitopes and is highly immuno
journal: Sel skokhozyaistvennaya Biologiya