text:
In what appears to be extremely bad news for the international community, recent reports suggest the possibility of North Korean-Iranian cooperation in Pyongyang's new launch facility.
Recent satellite images published by the thinktank 38 North of the US- Korea Institute at the School of Advanced International Studies (SAIS) specify the latest activities at the new facility at Musudan-ri in North Korea.
The new construction appears to be an Iranian model of the recently constructed horizontal assembly building of Semnan launch complex in Iran which is equipped to handle a new large liquid fuelled rocket.
The latest available images which were taken on 29 April indicate the quickly reviving construction of the new launch facilities. The construction has been underway since mid 2011. The facility is to allegedly provide more support for the future rocket launches.
"Present are a flame trench orientated slightly downhill toward a stream, foundations for fuel and oxidizer tank buildings that bracket the flame trench, a dam up-stream connected by a pipeline to a water tank at the main facility and three other completed buildings. The most unusual feature is a white circular structure that surrounds where the rocket will stand when erected on the pad, the purpose of which is unclear," said the analysis report penned by the foreign weapons expert Nick Hansen.
The available materials clearly point out that the facility will be more capable of launching liquid fuelled space launch satellites or even ballistic missiles with intercontinental range.
However the report also added that it would be premature to come to any conclusion but it is not possible to rule out the possibility of the cooperation between the two notorious countries.
"The North Korean facility, however, seems to have a similar layout, with labs and what appear to be administrative offices on the sides of the high bay building. But there are also clear differences; for example, the "T" building at the end of the North Korean structure is not present in Iran," added the report.
The two countries have worked together in the past in many of the ballistic missile and space launch vehicle development programmes which possibly forms the premise of the assumption.

domain: politics


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Direct flights are available for this route on the following days: Sunday, Tuesday, Wednesday, Thursday, Friday, Saturday.
This flight path from Glasgow Prestwick to Newquay St Mawgan was viewed by 935 users in the past month. Keep an eye out for changes in search interest prior to high and low season.
The rainfall in Prestwick is generally more than the rainfall in Newquay. This will continue over the next 9 days.
You can imagine temperatures to be in the 19 °C range in July. The weather in Newquay is generally the same as Prestwick's. As flight availability is subject to become more limited as peak season approaches it is ideal to book in a timely manner.

domain: geography


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The famous Chinatown in Singapore is a place filled with colorful markets, old temples, and museums that tell the rich history of one of Southeast Asia’s most progressive countries.
There are so many things in Chinatown that sets it apart from the rest of the city: the town boasts of low-rise buildings influenced by the Singaporean culture, traditional cuisines, and symbolic landmarks that show how proud the place is of its heritage. Heavily decorated Chinese, Buddhist, and Hindu temples always invoke awe, while the museums and the colorful markets lining up the street invite visitors to discover them. Making a stop at Chinatown is a great option if you want to see and learn more about Singapore.
Chinatown is located at Pagoda Street, Singapore.
Opening Days and Hours
Chinatown is open anytime. However, establishments only start to open at 11:00.
Entry Fee and Bookings
Most of the sights at Chinatown charge no admission fee but some places like The Red Dot Museum, Singapore Coins and Note Museum, Chinatown Heritage Center, and Duxton Skybridge charge entrance fees ranging from USD 6 to USD 10.
How to Get There
- From the city center, board a train to Chinatown station.
- Take Exit A to Pagoda Street.
- Solo Travelers/ Backpackers
- Group of friends
Popularity and Best Time to Go
Most of Chinatown’s establishments and attractions do not open until after 11:00. The best time to visit the place is at 12:00.
- Little India
- Gardens by the Bay
- Sentosa Island
There are a great number of restaurants around Chinatown tourists can choose from according to their budget. Tong Heng, and Akbar restaurant for cheap meals; Hometown Restaurant, and Tian Jeen Fong Kee for mid-priced dishes; and Blue Ginger, and Da Paolo for fancier meals.
Find a place to stay among the many hotels available in Chinatown like Amara Hotel, M Hotel, Hotel 1929, Keong Saik Hotel, and Beary Best Hotel which offer accommodation at rates ranging from USD 50 to USD 250.

domain: travel


text:
You probably know her from Disney Channel's Girl Meets World—though you might also recognize her from the red carpet or from the front row at Fashion Week: Actress and singer Sabrina Carpenter has been carving a space for herself in the industry and using fashion to make her presence known.
For Carpenter, establishing a strong point of view in fashion at this point of her life is essential to the legacy she wants to look back on later. "You really remember where [someone was] in their career based on their look and the trends that they started. That's so powerful," the 18-year-old tells Glamour.
She's still working out exactly what she'd like her style to be defined as, she says, but taking ownership of her look is a consistent part of the equation. That translates into her taking on her very first fashion collaboration—with Aéropostale, which launches on April 8.
She's been an ambassador for A´éropostale since the holiday season. With this design partnership, she wanted to focus on accessibility, her 20 million followers across platforms always top of mind: Everything in the festival-ready collection will retail for under $50 and draws inspiration from her tour merchandise, which she says she also designs. Quadruple threat, much?
Then there's how Carpenter presents herself, onstage and off.
She's been working with stylist Jason Bolden since last fall, a partnership she says has helped explore different types of styles she might not naturally gravitate toward. "It's always so, so hard to find people that you trust enough to allow them to pick out things for you that you would never pick out yourself," she tells us.

domain: entertainment


Bull is nearing the end.
And well … it’s not bullish.
Lead star Michael Weatherly confirmed the news on Twitter late Tuesday, jumped to his Twitter account and wrote:
Hello everyone! It was my privilege to play Dr. Jason Bull, but after six seasons of great stories, I decided it was time to pursue new creative challenges and end his story.
This message concludes the actor:
We are honored to work with this talented cast, crew and writing / production team to help reinvent the legal drama.
Please look forward to the finish of the big series …
Thank you from the bottom of my heart. You will always be part of our bull family!
There are 12 episodes of Bull left.
The Series Finale will air in May.
Weatherly Left Smash CBS struck NCIS to secure his own series that was actually based on Dr. Phil McGraw’s life before becoming a talk show host and his work as a trial consultant.
It premiered in September 2016, and two years later, Eliza Dushku was caught up in a big controversy after being released in a sexual harassment allegation against Weatherly.
According to a New York Times report, Dushku claimed that Weatherly often spoke of her appearance in front of the cast and crew at the time.
She also said he made remarks about rape jokes and the trio.
Shortly after Dushku confronted the star about his actions, she was written down from the series.
CBS finally resolved the issue with Dushku for the reported $ 9.5 million and issued the following statement:
“Dushku’s claim is an example of how we continue to commit to a culture defined by a safe, inclusive and respectful workplace, while our work is not done yet.
“The settlement of these claims reflects the projected amount that Mr. Dushku would have received for the balance of a series of periodic contracts and was determined by a mutually agreed mediation process at the time.”
Weatherly also tackled the scandal in his own statement.
“In the process of taping our show, I made some jokes that mocked some lines of the script,” he said in late 2018.
“When Eliza said she wasn’t used to my words and humor, I regretted having offended her and immediately apologized.
“After further pondering this, I better understood that what I said was neither interesting nor appropriate. I’m sorry, but I regret the pain that caused Eliza.”
Meanwhile, CBS acknowledged the end of the bull by saying:
“For six seasons, Bull has established himself as a winner of the assessment with a fresh view of the judicial process that has never been seen on television.
“Thanks to the talented cast, Michael Weatherly, Geneva Carr, Yara Martinez, Jaime-Lee Kirchner, Christopher Jackson, MacKenzie Meehan, the amazing creative team led by Kathryn Price and Nicole Millard, and the hard-working crew. These innovative stories.
“We also thank our loyal viewers and look forward to providing the final run of a fascinating episode full of humor, intelligence and twist that made the drama a fan favorite.”
Bull: Canceled by CBS After 6 Seasons! Source link Bull: Canceled by CBS After 6 Seasons!
The post Bull: Canceled by CBS After 6 Seasons! appeared first on California News Times.


text:
What a lot of great inspiration from all the Designers and fabulously generous prizes from the sponsors!
Did you win one of the prizes up for grabs?????
Click the link here and see if your name is there.
Congratulations to all the winners!
I just wanted to say a huge thank you to everyone who too time out of their busy day to hop along with me and leave me some very lovely comments, I read them all and they really do brighten my day. xx
So, the really big question is who won Lesley's Gorgeous Colouring PDF??????
You know it may say Easter, but I have to tell you that there are eggs there for very occasion!
Here's a few I have coloured up:
Ok, so I let Radom Org pick a winner out of the 27 who commented and it chose Cara Malmrose.. Cara please message me with your email details so we can get your prize sent.
Thank you all again and thanks so much for visiting my blog.
Until next time, Happy Crafting.

domain: artistic


text:
ESPN Sunday Night NFL was one of the many football games released back in the day. It features all 28 official NFL teams and their respective logos, but doesn't feature a license from the NFLPA, so there are no actual players names in the game.
There are four different game modes to choose from: Playoffs, Single Game, Season and another season mode that lets the player act out the 1994 NFL season schedule. It also had variable weather conditions that the players on the field would react to and a battery back-up to save games. It also featured Chris Berman, who not only graced the front cover but also gives commentary as well.

domain: hobby


text:
April 26, 2002
- A story in the Metro section Wednesday mistakenly reported that internal police investigators had found child pornographic images on a computer in the Wood District station on the Near West Side. In fact, one computer was seized from the district, but an analysis of that computer has so far not turned up any suspect images, police officials said. - An item in the Almanac column Wednesday gave the wrong year for the deadly fire aboard the submarine Bonefish off the Florida coast.
August 11, 1990
On Aug. 11, 1860, the first successful silver mill began operating near Virginia City, Nev. In 1896 Harvey Hubbell of Bridgeport, Conn., received a patent for an electric light socket with a pull chain. In 1902 Oliver Wendell Holmes was appointed associate justice of the U.S. Supreme Court. In 1909 what is believed to be the first radio SOS sent by an American ship was transmitted from the liner Arapahoe, whose engines were disabled off Cape Hatteras, N.C. In 1924 for the first time, the nominees for U.S. president were filmed for newsreels.
December 19, 2000
Steven Soderbergh's film "Traffic," a somber investigation of the drug trade, has been voted best film of the year by the New York Film Critics Circle. The film, which features an ensemble cast led by Michael Douglas, opens in New York on Dec. 27. Soderbergh, who also oversaw the hit Julia Roberts film "Erin Brockovich," was voted best director for his work on both movies. And Benicio Del Toro, who plays a Mexican policeman in "Traffic," won for best supporting actor. The best actor award went to Tom Hanks for his portrayal of a FedEx worker stranded on a deserted island after a plane crash in "Cast Away."
May 19, 2002
From the raw reality of the Ash Can School era to the far-out reaches of Abstract Expressionism, New York's celebrated Art Students League was one of the major forces in American art in the 20th Century. New York's UBS PaineWebber Art Gallery is paying homage to that pervasively important institution with an exhibition of works by some of its most notable alumni, whose ranks include John Sloan, George Bellows, Georgia O'Keeffe, Paul Cadmus, Alexander Calder, Jackson Pollock and Roy Lichtenstein.
January 10, 1988
Once it was Denver's finest bordello and gambling parlor. There were rumors that as a convenience to guests who would rather come and go unseen, a secret underground passage led across the street to the fabled Brown Palace Hotel and-perhaps-even beyond to the basement of the State Capitol itself. The Brown Palace Hotel is still there, as fabulous as ever. But these days the building known as Denver's historic Navarre attracts a different, less reticent class of visitor-lovers of Western art. Tucked in among the high-rises of downtown Denver, the Museum of Western Art is one of those little museum gems that tourists are apt to overlook and that even few natives know about.
January 28, 2009
On Jan. 28, 1547, England's King Henry VIII died in London; he was 55. In 1853 Cuban revolutionary Jose Marti was born in Havana. In 1873 French novelist Colette was born Sidonie-Gabrielle Colette in St.-Sauveur-en-Puisaye, France. In 1878 the first commercial telephone switchboard went into operation, in New Haven, Conn. Also in 1878 the first daily college newspaper, the Yale News, began publication in New Haven, Conn. In 1892 film director Ernst Lubitsch was born in Berlin.
January 28, 2010
On Jan. 28, 1547, England's King Henry VIII died in London; he was 55. In 1912 painter Jackson Pollock, one of the foremost exponents of Abstract Expressionism, was born in Cody, Wyo. In 1915 the Coast Guard was created by an act of Congress. In 1916 Louis Brandeis was appointed by President Woodrow Wilson to the Supreme Court, becoming its first Jewish member. In 1922 the Decatur Staleys professional football franchise was renamed the Chicago Bears. In 1973 a cease-fire officially went into effect in the Vietnam War. In 1982 Italian anti-terrorism forces rescued U.S. Brig.
February 16, 2001
Here are selected capsule reviews of movies in current release. = Among the new movies that open this week. = Faithless (star)(star)(star)(star) With magical, brilliant artistry, this film depicts an episode in Ingmar Bergman's life based on an adulterous relationship. Written by Bergman and directed by Liv Ullmann, it's one of the screen's finest portrayals of infidelity and its consequences. Erland Josephson and Lena Endre star. R (sexual content, some nudity and language)
September 22, 2002
Life in modern times is examined in a context that mingles chaotic and contemplative street scenes of Chicago, New York, Tokyo and Dusseldorf with intense portrait studies of families and individuals as well as with gigantic images of lush rain forests in Asia and South America in a new show of work by German photographer Thomas Struth that opened last week and continues through Jan. 5 at Los Angeles' Museum of Contemporary Art, 250 S. Grand Ave....


Miniature Pressure Wave Switch (adjustable, 0.8 - 8.0 mbar)
The simple and compact design of this adjustable PE converter allows its use in various applications such as medical technology, dental treatment units, robotics, analysis systems, process and safety engineering. Easy mounting and high functional reliability are other advantages. By using silver contacts a long lifetime can be ensured even at high switching frequencies.
|Pressure range||>0.8 - 8 bar|
|Type of electric connection||Plug contacts|
|Type of pneumatic connection||M5|
|Ambient temperature||-10 ... +60 °C|
|Materials||Contacts: Ag 5|


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Patient families, friends and the general public are invited to take their first look inside Akron Children’s Hospital’s new $180 million Kay Jewelers Pavilion at a community open house from 1 to 4 p.m. on Sunday, April 26.
How will healthcare look in 20 years? That would be nice to know as we build now Dr. David Chand on the kaizen process
As Akron Children’s Hospital moves forward with its $200 million campus expansion, a crystal ball would come in handy. With health care reform, changing demographics, and other uncertainties, our goal is to build flexibility into our design in every way possible. We can make educated guesses regarding future patient volumes and acuity, reimbursement levels, the […]
As our teams assemble to discuss and collaborate on best practices and design of Akron Children’s Hospital’s new $200 million critical care tower, participants continue to experience “Ah, ha!” moments.
Akron Children’s Hospital is launching a $200 million expansion of its Akron campus to meet the current and future needs of children and their families.

domain: medical


North Korea launched a long-range ballistic missile early Thursday, according to officials in Japan and South Korea, the same day leaders from those two countries are due to meet in Japan for the first such summit in 12 years.
At least one unidentified ballistic missile was fired into the waters off the east coast of the Korean Peninsula early Thursday morning local time, according to South Korea’s Joint Chiefs of Staff.
Japan’s Defense Ministry also confirmed a launch, estimating that the missile would fall outside its Exclusive Economic Zone (EEZ), approximately 550 kilometers (341 miles) east of the Korean Peninsula.
The South Korean military was maintaining a full readiness posture while closely cooperating with the US, the JCS said.
South Korean President Yoon Suk Yeol is due to arrive in Japan Thursday for a summit his office has hailed as “an important milestone” in the development of bilateral relations.


text: This finca is situated in the south east of the island, close to the centre of Colonia de Sant Jordi, an area, in which guests can discover some of the most beautiful unspoiled beaches in the ecosystem of Mallorca - Es Trenc, Es Carbó, Es Peregons petits, Es Coto, Es Caragol and El Marques to name but a few. Restaurants, bars, clubs and shops can be found a 5-minute drive from the finca and the centre of Ses Salines is approximately 4 km away. The air-conditioned hotel comprises a total of 6 rooms. Services and facilities at the hotel include a safe, a restaurant, a dining area and a TV room. Wireless internet access in public areas allows guests to stay connected. There is also a garden. Those arriving in their own vehicles can leave them in the car park of the accommodation. Travellers can also use the bicycle storage facilities. Active guests can make use of the bicycle hire service to explore the surrounding area. Rooms feature air conditioning, central heating, a kitchen and a bathroom. For additional comfort, a terrace features as standard in most rooms. Guests can enjoy a good nights' sleep on the sofabed. Further bookable amenities include separate bedrooms. Extra beds can be requested. A safe and a minibar are also available. Additional features include a fridge, a cooker, a microwave and a tea/coffee station. Little extras, including internet access and a TV, contribute to a great stay. Amenities in the bathrooms include a bathtub. A hairdryer is also available. The establishment offers numerous leisure facilities, including a pool and an outdoor pool. A terrace, a sun terrace, sun loungers and parasols are available. Those wishing to enjoy sports whilst on holiday can enjoy on-site horse riding. For a fee, biking/mountain biking is is also available. The welllness area offers pure relaxation, with services including massage treatments and a solarium. For a fee, a beauty salon is available to travellers. Copyright GIATA 2004 - 2016. Bed and breakfast is bookable. It is also possible to order breakfast. The following credit cards are accepted at the hotel: VISA and MasterCard.


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Shelbi and Alex were an amazing couple to have an engagement session with. I learned a few things about during our time together. Shelbi is from Nebraska! I have never been to that state so I was super excited to hear that. I always love to meet new people from different parts of the country.
They both attend Olivet Nazarene University. Which is where they met!
Our session took place at the beautiful Kankakee River State Park. I go here a lot for photoshoots, but each one is different! The land is so big, with so much diversity. I could shoot a million more couples here and still see how special EACH couple is with their OWN style and love.
We had a quite scare with the weather. We didn’t know if the rain was going to be light or harsh. Turns up we finished just a few minutes before the storm brewed in. I was so happy! There were a few sprinkles here and there, but I just had the chance to use my handy dandy bubble umbrellas! Those turned out to be my FAVORITE shots.
Congratulations to these two sweet humans. Thank you for working with me to create these beautiful photos! xoxo
If i had to pick just ONE photo from our session together. This would be it. Anyone else agree how sweet and wonderful it is?!
You wouldn’t think a photo of you walking together would be beautiful. But you guys it really is!
Here we had some fun with the focus 🙂 Variety is my favorite!
I just love taking my couples into the fields. They create texture and character.
And I might randomly tell you to dance together! I love the natural expressions and happiness from this.
Now it’s time to bring in the Umbrellas. I am obsessed with these!


Central Venous Access & Dialysis
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Medical grade PVC tubing for higher biocompatibility. Advantage of built-in Heparin and Saline line. Extra corporeal blood circuit tubing set, used during dialysis. Low Priming Volume. Ease of use.
Suitable for performing peritoneal dialysis in patients of all age groups. Perforated open-end catheter manufactured from polypropylene. Stainless steel trocar is provided to facilitate smooth penetration.
Clear Transparent & Flexible built in Chamber with sharp and easy piercing Spike. Special designed for 'Y' shaped transfusion set for administrating dialysis solution. Provided with two upper control clamp to facilitate the attractive change of solution bottles.
Super smooth kink resistance tubing ensures uniform flow rate. To connect blood lines of the blood vessels through needles when dialysis is carried out through an internal Fstula.


text:
Tonight we will see the 2014 version of Survivor Series and it has been shaped up to be one of
the most controversial of all time.
It will be the 27th Survivor Series of WWE history and the 2nd straight event where the WWE
Universe will not be treated to a WWE World Heavyweight Championship match.
WWE World Heavyweight Champion Brock Lesnar has been absent from our screens since
defending his title against John Cena at Night of Champions.
With the big stipulation in place ahead of the Team Cena vs Team Authority five-on-five
elimination tag team match, could there be bad news in store for the champion?
Should Team Authority lose tonight, they will officially be out of power and depending on who
takes over control of the WWE, Lesnar’s cushion of protection could go with them.
- Top 10: Greatest Survivor Series moments
- A loss at Survivor Series could see the divas division crumble
- The WWE have a big problem with Rusev at Survivor Series
Earlier this year we saw Daniel Bryan get injured and become victim of the 30-day defence rule which states that the champion must defend his title every 30 days or he has the possibility to be stripped of the title.
Lesnar has now not even been seen for almost 2 months and clearly the Authority are holding
back on imposing the rule on the Beast Incarnate.
However if the Authority is dethroned and a predominantly face character takes over the
company, what will that mean for Lesnar.
Some of the names being announced such as Mick Foley would certainly fill that role and would have the power to bring Lesnar back into the picture.
Lesnar’s vacation may soon be over and it is widely believed that Team Cena will emerge
victorious at Survivor Series.
He has however already been booked onto RAW shows in December and is rumoured to be
facing John Cena at TLC next month.
Who knows who will take over or how Lesnar will be re-introduced to the ring, but I think it is fair to say that we are not far from the Beast’s return.
The stakes are high at Survivor Series for both teams, of course the Authority are out of power
should they lose but Team Cena are in it for more than just their reputations.
Triple H announced Friday night on Smackdown that four of the five members of Team Cena will be fired if they lose on Sunday.
Ryback, Big Show, Dolph Ziggler and Erick Rowan could all be set to take a bit of time off of our screens should they lose at Survivor Series.
As for John Cena himself, he won’t be fired but he will receive a lot of punishment at the hands of the Authority until he quits which if history has proven anything, just won’t happen.

domain: sports


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Last week we learned all about the different kinds of fruit desserts, so today we’re putting our new vocabulary to good use!
By throwing all caution, logic, and common sense to the wind and jumbling them all up in one dessert.
It’s fine because deep dish berry cobbler pie is worth breaking all the rules!!
And honestly, by making a franken-dessert like this you’re really just ensuring you get the best of all worlds – crispy flaky all butter pie crust + juicy berry filling + soft and sweet biscuit topping.
Add ice cream and there’s basically nothing better in life. I can control myself around most desserts that I make and limit my consumption to the weekend, but pie is my one weakness. Fruit pies, especially. They are my ultimate favorite!
This recipe comes from my pie bible, The Book on Pie by Erin McDowell. I’ve gushed about it excessively in previous pie posts, but to sum it up – IF YOU LOVE PIE THEN YOU NEED THIS BOOK.
Of course, it has a few unique steps to make this pie extra special.
First, you start with Erin’s classic, fabulous all-butter crust. For all of her pies, I make a crust recipe and a half because I always like a little extra when it comes to my crust. It gives me a little wiggle room for rolling it out and ensures I’m not trying to press odds and ends together to have enough to line the pie plate.
Next, you’ll make your filling! Mix the berries with sugar and a little bit of lemon juice to macerate for about an hour. This helps them to release any excess juice, which you’ll strain and reduce down to a thick syrup. Doing this helps the filling to set up better and is insurance against having a soggy, watery, pie mess. Use the berry macerating time wisely and make your biscuit topping, this way you’ll be ready to go once everything is done.
Once the berries are drained and juices reduced, you’ll mix the berries with brown sugar, flour, and some cinnamon. Add in the reduced juices and pour the filling into your prepared pie crust. Top it with dollops of the biscuit topping and you’re ready to bake.
This pie is full of sweet tart berry flavor with a crispy buttery bottom crust and a soft, fluffy, barely sweet biscuit topper. As I said, it’s the best of all worlds and the ideal summer dessert, especially when served with ice cream or barely sweetened whipped cream.
What are you waiting for? It’s time to get baking!
- 1.75 cups + 2 tbsp (225 g) all purpose flour
- ¼ + ⅛ tsp fine sea salt
- 12 tbsp (170 g) cold unsalted butter, cut into ½-inch cubes
- 6 tbsp (90 g) ice water, plus more as needed
- 1 cup (120 g) all purpose flour
- ¼ cup (53 g) packed brown sugar
- ¾ tsp baking powder
- ¼ tsp baking soda
- ½ tsp fine sea salt
- 4 tbsp (57 g) cold unsalted butter, cut into ½-inch cubes
- ¼ cup (60 g) buttermilk
- 1 large egg
- ½ tsp vanilla extract
- turbinado or demerara sugar, for sprinkling
- 6 heaping cups (1010 g) berries (I used a mix of strawberries, raspberries, and blackberries. If using strawberries, slice them otherwise berries can be left whole)
- 1 tbsp (15 g) fresh lemon juice
- ⅓ cup (66 g) sugar
- ⅓ cup (71 g) packed brown sugar
- ⅓ cup (40 g) all purpose flour
- ¾ tsp ground cinnamon
- ½ tsp fine sea salt
- 2 tsp vanilla extract
- In a large bowl, whisk together the flour and sea salt. Add the butter cubes to the bowl and toss to coat in the flour, then use your fingers or a pastry cutter to cut the butter into the dough until the largest pieces are the size of peas.
- Slowly pour in the water, a tbsp or two at a time, mixing the dough lightly with your hands after each addition. Add only enough water until the dough starts to come together. You may need a few more or less tbsp than the recipe states.
- Once the dough comes together, form it into an even disk, about 1-inch thick. Wrap tightly in plastic wrap and refrigerate for at least 30 minutes or up to 2 days.
- Once chilled, roll out your dough on a lightly-floured work surface to a 12-inch circle and then fit into a 9-inch deep dish pie plate, crimping the edges as desired. Dock the crust all over with a fork and place in the refrigerator to chill for at least another 30 minutes.
- To parbake the crust, heat oven to 425F. Line the chilled crust with parchment paper and top with pie weights to fill the crust entirely. Place in the oven and bake for 15-17 minutes, or until the edges are set and just starting to brown. Remove from the oven and lift the parchment and weights out of the pie shell. Return the crust to the oven and bake for another 3-5 minutes, or until the bottom appears set. Allow to cool completely.
- In a large bowl, whisk together the flour, sugar, baking powder, baking soda, and salt. Add the butter and cut it into the flour mixture with your fingers or with a pastry cutter until the mixture resembles wet sand.
- In a bowl or measuring cup, whisk together the buttermilk, egg, and vanilla. Make a well in the center of the flour mixture and pour the liquid mixture into it. Use a spatula to stir the mixture until it comes together. Set aside in the fridge until ready to use.
- In a large bowl, toss the berries with the lemon juice and sugar. Allow to macerate for at least 1 hour or up to 3 hours. Toss a few times during the first hour to ensure the berries are evenly coated in the sugar/lemon juice.
- Place a strainer or colander over a medium pot and pour the berries into it, allowing any juices to drip into the pot. Press down gently on the berries to get any excess juices out. Transfer the berries to a bowl.
- Set the pot with the berry liquid over medium heat and bring to a simmer. Cook until the liquid has reduced to ¼ cup. Remove from the heat.
- Meanwhile, heat oven to 375F with a rack in the lower third of the oven.
- In a small bowl, whisk together the brown sugar, flour, cinnamon, and salt. Add the brown sugar mixture to the berries and toss to combine. Pour in the reduced juices and the vanilla extract. Toss again to combine.
- Pour the filling into the cooled crust and spread into an even layer. Top with dollops of the biscuit topping, leaving some of the filling around the edges exposed. Sprinkle with the demerara or turbinado sugar.
- Place the pie on a parchment-lined baking sheet and transfer to the oven. Bake for 35-40 minutes or until the filling is bubbling and the biscuit topping is a deep golden brown. Allow to cool completely before serving.


text:
Citrix has announced new features in Citrix Workspace to help workers be more efficient. The solution now includes an intelligence feed and personalized workflows that will allow users to automate tasks.
“At the end of the day, employees want to master their craft,” said Tim Minahan, executive vice president of strategy and chief marketing officer at Citrix. “With Citrix Workspace, employers can empower them to do so by freeing them from soul sucking tasks so that they can focus on building their skills and engaging work.”
D2iQ launches new global channel program
D2iQ has launched a new global channel program that was designed to allow cloud native growth within the reseller community. This program will enable channel and resell partners to unlock cloud native revenue streams and expand market reach.
“Partners are the key factors to success when it comes to customers being able to manage and mitigate risk on their cloud native journey; that’s why D2iQ has launched its global channel program,” said Joe Taborek, chief revenue officer at D2iQ. “This program builds structure for our growing channel and resell business while also showing our commitment to the cloud native community. The goal of this program is not only to empower our channel and reseller community but also to support our customers as they make their journey to cloud native.”
Stephanie Fohn joins NeuVector as CEO
NeuVector has appointed Stephanie Fohn as its new president and CEO. Fohn will also be joining the company’s board of directors. The company’s co-founder Fei Huang will become chief strategy officer.
Previously, Fohn served as the CEO of WhiteHat Security where she expanded the company from eight employees to over 300. She also helped Remotium be acquired by Avast Software in her time there, and most recently was the CEO of Rundeck.
“I’m thrilled to join NeuVector at this important stage in the company’s growth,” said Fohn. “The NeuVector container security platform is extremely robust and highly-valued throughout the market and by customers. We see tremendous opportunity as companies continue to move to the cloud and adopt cloud-based application strategies. Our goal is to take advantage of these market opportunities as we continue to grow NeuVector.”
GigaSpaces 15.0 now available
GigaSpaces has launched version 15.0 of its platform. GigaSpaces 15.0 includes features that will help businesses derive better value from their data.
This version introduces a new monitoring and administration tool called Ops Manager that provides visibility into system components that run machine learning models. In addition, InsightEdge, which is included in GigaSpaces 15.0 now includes AnalyticsXtreme Batch Indexing, which automates data access and storage and allows administrators to move data between frequent (cold data) access and infrequent (archive data) access tiers in data lakes and warehouses.
Finally, it provides a native smart space client in Kubernetes which supports CRUD operations, task execution, event-driven analytics, and automatic load balancing.

domain: news


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Bly has lived in a home previously and was housetrained and ok being left alone for a few hours. He gets on well with other dogs and cats although he does like to chase them.
Type of home needed
Bly is a lively boy who is looking for an active home with new owners who will have the time to train and exercise him. He could live with another dog in his new home following a successful mix at the centre. He could live with children of 8 years or over who will go steady with him until he gains in confidence as he can be nervous at times. He is strong on the lead so will need new owners who are able to manage him.
More about me
Can you offer Bly his home for life?

domain: pet


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Brand Comparison: Indigenous Collection
This page is part of our Puzzle Brand Comparison. You can find the overview page here
Last Updated On: March 10, 2022
Summary: Indigenous Collection by Cap is a Canadian puzzle brand that specializes in indigenous artwork. Part of the proceeds of each puzzle sale actually go back to the artist! The puzzle box is sleek- slim and tall, with the entire image on the front, and information about the artist on the back. The pieces come in a resealable plastic bag, are a standard grid cut, and have a great fit. Unique and colorful, you can’t go wrong with a puzzle from Indigenous Collection!
Image Categories: Original artwork, Native American art, animals, landscapes, and more
Cut: Ribbon cut with varied piece shape
Front Finish: Semi-glossy
Piece Backing: Plain chipboard
Piece Thickness: 1.9mm or 0.07in
Puzzle Dust Level: Low
Piece Fit: Snug for circular puzzles. You can move 3-4 piece sections for rectangular puzzles.
Piece Counts Offered: 72, 500, 1000
Packaging: Small tin boxes for 72 pieces, medium squares for 500 pieces, medium rectangles for 1000 pieces. Boxes are sealed with plastic stickers. Pieces come in non resealable bags.
Box Bonuses: Information about the artist is featured on the back of each box
What Comes in the Box
Examples of Piece Shapes
Front and Back of Box
Sides of Box
Completed Puzzle Image


There are few places in Pinal County that are quite as serene as Boyce Thompson Arboretum.
Located just on the outskirts of Superior, past the Tonto National Forest, the arboretum is home to 19,000 different kinds of plants across 135 acres.
Plant collections come from across the globe, derived from places close to home like the United States and Mexico, but also from regions as far as China and the Mediterranean.
"We are Arizona's largest and oldest botanical garden," said Taylor Krassa, marketing coordinator. "Overall we have 393 acres and about 5 miles of trails. We have collections from about 13 different countries, representing areas in deserts from around the world."
Founded in 1924 by Col. William Boyce Thompson, the arboretum started with Thompson's dedication to conserving plant life following two trips to Russia before and after its civil war of 1918.
At the time of its establishment, the arboretum was the first nonprofit research organization in Arizona.
In the years and decades to follow, plenty of research would be conducted at the expansive botanical garden with scientists regularly arriving to stay overnight and spend their days studying the plants housed at the facility as early as 1925.
More than five decades later, the nonprofit — at the time operated through a partnership between the arboretum, Arizona State Parks and the University of Arizona — published its first issue of "Desert Plants Journal." The biannual publication spotlights research and information about desert plants, landscapes and the environment.
For area residents and visitors alike, the arboretum is one place where people can see many of those plants in-person.
"It's definitely one of the spots in Pinal County where you can see an array of flora and fauna," said Sharon Elliott, director of marketing and communication. "You might catch a glimpse of a northern cardinal or if you're lucky enough you might see a fox. We've even had some bobcat sightings. It's kind of like that one spot in Pinal County where you're gonna get that full, immersive Arizona nature experience."
Although open year-round, the arboretum operates on seasonal hours, 6 a.m. to 3 p.m., beginning in May and continuing through September. Final admissions into the gardens end one hour before the facility closes. In addition, in June and July, the facility is closed on Tuesdays.
Elliott recommends that those planning a visit in the summer prepare accordingly for spending time outdoors. She suggests wearing sunscreen, having plenty of water and wearing attire and shoes that are comfortable and safe for walking and hiking outdoors.
Families can also enjoy their lunch at the arboretum's picnic area, though it's important to keep in mind that grilling or overnight camping are not allowed on the grounds.
Sitting at an elevation of about 2,431 feet, the arboretum may also just be the right reprieve for those looking for a break from excessively high summer temperatures.
"Typically we are about eight to 10 degrees cooler than the Valley depending on the day," said Elliott. "With the added shade from the gardens and our rock features and things like that, it can be a lot cooler to come and spend the day out here and get a break from the sun and the heat."
Among the most popular selections at the arboretum is the newest collection, the Wallace Desert Garden, which opened to the public in October 2020. The garden features thousands of international desert plants donated from the collection of Henry B. Wallace, first situated in Scottsdale.
In addition to the arid plants on display, the garden also features 1.5 miles of trails.
Beginning in June, the arboretum will also bring back Arb After Hours. The collaboration with the Superior Chamber of Commerce gives attendees an opportunity to enjoy a couple of drinks while spending time in some of the arboretum's gardens.
"There is just so much variety within the gardens because we are so expansive and we have so many different collections from different areas," said Krassa. "There is really something for everyone."
For more information about hours, admission and upcoming events visit: btarboretum.org/visit/.


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Peterborough Real Estate Related Information
Peterborough is a city on the
Otonabee River in Ontario, located just 112 km outside of Toronto. As the gateway
to the Kawarthas, "cottage country", Peterborough is known as the recreational
region of the province. It is named in honour of Peter Robinson, an early
Canadian politician who oversaw the first major immigration to the area.
Peterborough is the second safest urban
city to live in Canada. Its breadth of nature makes it truly one-of-a-kind.
At BargainMax, we offer the
latest MLS® listings for resale homes and pre-construction projects
in Peterborough. We pair this with our unprecedented customer service, and
great advice to give you for your investment properties in Peterborough.
inquire about homes for sale in Peterborough, please contact us.
What is the average home price in Peterborough?
home price of detached homes in Peterborough: C $540,500
The above data is
based on the Realtor.ca web site. Above
average prices are for information purposes only and may change at anytime.
What are the popular neighborhoods and areas to live in Peterborough?
Please refer to the Peterborough web site for more
What are the major cities around Peterborough?
Top cities around Peterborough are: Bobcaygeon,
Lakefield, Millbrook, Lindsay, and Hastings.
What is the average rent of homes in Peterborough?
Average rent of 3-bedroom homes in Peterborough
is approximately C $1995 + utilities.
What is the average rent of a basement in Peterborough?
Average rent of 2-bedroom legal
basement in Peterborough is approximately C $1712 + 30% of utilities.
What is the average rent of an apartment in Peterborough?
Average rent of a 3-bedroom apartment
in Peterborough is approximately C $1960 + utilities.
Please note that rental of various
property types varies based on the number of bedrooms, community area, and we
have taken the average of rental value. This data is only for information
purposes and may change at anytime.
Demographics and population of Peterborough
According to Wikipedia’s website, Whitby boasts
a population of over 121,721 people and growing.


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Motorists plying the Asaba-Onitsha expressway yesterday commended the Federal Government, the state government and private individuals who have contributed to the ongoing rehabilitation of the failed Okwe Junction, Asaba, portion of the road.
One of those who spoke to The Guardian, Mr.Jasper Onwuka, a passenger in one of the buses from Lagos heading to Onitsha, said: “The rehabilitation of the failed portion of this popular road is heartwarming and a thing of joy to all.
The traffic gridlock before the rehabilitation was really agonizing as it made travelling to the eastern part of the country, a nightmare.”
Also, an Asaba based transporter, Mr. Emeka Titus said the rehabilitation has made their business less frustrating and more rewarding.“Before now, we spent hours on the road just because of the failed portion, but we are happy that it has been worked on.
But my advise is that we develop a maintenance culture and not wait till the whole thing gets worse.
It would even be cheaper if attention is paid when the road failure is just beginning with mere potholes rather than when large stretches have collapsed and pose serious problem to motorists.”


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Science and pot lovers will appreciate The Molecule Collection THC Socks. The molecular structure of THC in green on black socks. THC is the chemical responsible for most of marijuana's psychological effects. After weeding through your sock drawer, you have found the perfect pair. Approximately fits men's shoe 8-12.5.
70% Cotton, 27% Nylon, 3% Spandex

domain: chemistry


Mineral Resources Ltd (ASX: MIN) shares are under pressure on Tuesday.
In morning trade, the mining and mining services company's shares are down almost 3% to $67.80.
Why are Mineral Resources shares dropping?
Today's weakness appears to have been driven by broad weakness in the lithium industry today which has overshadowed some positive news relating to another side of the Mineral Resources business.
An announcement this morning provided investors with an update on the Lockyer-5 conventional gas development well (L-5) located in the onshore Perth Basin.
According to the release, following an appraisal of the L-5 well, a sidetrack was drilled to a gas development well location that reached a total depth of 4,574m measured depth relative to the rotary table (MDRT). The Kingia Sandstone objective was successfully intersected at 4,424m MDRT.
The good news is that petrophysical analysis of Lockyer-5 Sidetrack-1 (ST-1) wireline logs has yielded "exceptional results" according to management. It revealed 27m of net gas pay with an average porosity of 18%.
Management notes that this is the highest quality reservoir encountered in all wells that have intersected the Kingia Sandstone across the onshore Perth Basin.
Preparations are now underway for a well test in early April to evaluate gas flow rates and composition.
Following the completion of operations at L-5, Mineral Resources relocated the drilling rig to North Erregulla-2 (NE-2). It is expected to reach a total depth of 3,492m MDRT in mid-April.
Management highlights that NE-2 is an appraisal well to test the quality and deliverability of the 47m net oil pay in the Dongara-Wagina Sandstone encountered in the NED-1 well.
Initial laboratory analysis of the oil recovered in NED-1 has provided promising indicators of high-quality oil. Initial wireline log analysis indicates an average porosity of 13%.
The NE-2 pay zone will be fully cored, followed by a full suite of wireline testing and a flow test to further define and unlock the considerable oil potential in the region.


LAPD Officer in Tyler Seddon video killed while on duty
By Alana Cerrone
One of the LAPD Officers who wished Tyler Seddon a “Happy Birthday” in a YouTube video was killed in a traffic accident in Beverly Hills.
Hollywood Division Officer Nicholas Lee and his partner were responding to an incident Friday morning when a large truck lost control and hit their patrol car.
Officer Lee was pronounced dead at the scene. His partner was sent to the hospital but is expected to be OK.
Lee was a 16-year veteran of the Department, and leaves behind a wife and two young daughters.
© WLNE-TV 2014


Enlarge / The Falcon 9 rocket on the launch pad, with its Starlink cargo tucked into the payload fairing.
If the climate and Falcon 9 rocket cooperate, the primary group of SpaceX Web satellites can be launched Wednesday night time from Florida. With a mass of 18.5 tons, it is going to be the heaviest launch of the corporate to date for the Falcon 9 rocket or Falcon Heavy.
Wednesday's rocket will energy 60 Starlink satellites, every weighing 227 kg, at 440 km altitude. That is the primary block of Starlink satellites for what ought to finally be a a lot bigger constellation. They’ll assist SpaceX consider its efficiency and take a look at a number of key methods. Within the coming months, these first satellites can be joined by six further launches carrying payloads of comparable measurement. These launches will deliver the constellation an preliminary "operational" functionality.
There isn’t a assure that every part can be alright, stated SpaceX founder Elon Musk throughout a teleconference with reporters Wednesday night time. "It's very tough," stated Musk. "There are lots of new applied sciences, so it's attainable that a few of these satellites should not working, there's a small chance that not all of those satellites will work."
Launch and publication
The launch window opens at 10:30 pm on Wednesday and the doorways shut 90 minutes later. A backup launch window will open on the similar time on Thursday. Present climate circumstances are anticipated to be 80% prepared for launch.
The primary a part of the launch can be acquainted to individuals who have already watched a SpaceX launch. This primary leg of the Falcon 9 has already flown twice earlier than and she is going to attempt to land on the drone. In fact I nonetheless love you within the Atlantic Ocean. The actual motion will happen roughly 1 hour and a pair of minutes after launch, when the second stage of Starlink satellite tv for pc deployment begins.
First 60 @SpaceX Starlink satellites loaded into the Falcon fairing. Tight match. pic.twitter.com/gZq8gHg9uK
– Elon Musk (@elonmusk), Could 12, 2019
To avoid wasting weight, every of the 60 satellites won’t have its personal set off mechanism, corresponding to a spring. As a substitute, Musk defined that the higher stage of the Falcon rocket will start a really sluggish rotation and that every of the satellites can be launched in flip with a special quantity of rotational inertia.
"It can virtually seem like spreading a deck of playing cards on a desk," stated Musk. There could also be contacts between Starlink satellites, he added, however they’re designed to cope with them.
After deployment, the satellites will start to energy their ionic readers and open their photo voltaic panels. They’ll transfer to an altitude of 550 km on their very own. Musk stated he was involved in regards to the deployment of photo voltaic panels and stated there are two totally different deployment mechanisms on the satellites for this function. He additionally said that the satellites had integrated a brand new expertise with thrusters in addition to phased array antennas that had not but been totally examined in house.
Satellites are designed to regulate prices. For instance, every will maneuver with Corridor impact thrusters, ionic thrusters by which the thruster is accelerated by an electrical subject. The traditional gasoline for such a propellant is xenon, which provides excessive efficiency. Starlink satellites, nevertheless, will use a special noble fuel: krypton. At decrease density, the gasoline tanks of satellites should be bigger and its efficiency is decrease than that of xenon. However krypton might be purchased at a tenth of the price of xenon, which is necessary if an organization desires to energy hundreds of satellites.
"It prices rather a lot lower than xenon," Musk stated of krypton. (He additionally joked, in response to a query from Ars about this gasoline, that the satellites can be resistant to the invasion of Superman's house world).
In the course of the name, Musk stated that every Starlink satellite tv for pc prices extra to ship into orbit than it prices to fabricate. The record value for a Falcon 9 launch is $ 62 million. If we contemplate reuse reductions and wholesale charges, it signifies that constructing Starlink satellites prices considerably lower than one million every
SpaceX is competing with about half a dozen different firms to develop a low latency, broadband Web community. One competitor, OneWeb, launched six of its personal satellites in February. However SpaceX appears to be properly forward of the remainder of the pack.
With six further launches and a complete of about 400 satellites, Musk stated the constellation was about to supply some preliminary connectivity to floor customers. A dozen launches would deliver "vital" connectivity, he stated, and 24 launches would offer a near-global service.
After a number of current fundraisers, SpaceX has sufficient capital to launch the primary 400 satellites and begin promoting service to telecommunication firms and governments wishing to serve densely populated areas. If there are any vital points with the deployment or efficiency of the primary 400 satellites, he stated that SpaceX ought to in all probability return to the capital markets.
Musk thinks that with time, Starlink will change into a industrial success for SpaceX and can allow it to realize its objective of constructing an autonomous metropolis on Mars. Potential start-up revenues attain about $ three billion a 12 months for the corporate, he stated, however capturing simply three% of the worldwide Web market may yield about $ 30 billion. "We see this as a means for SpaceX to generate income that can be utilized to develop an increasing number of superior rockets," he stated.
Musk has acknowledged the validity of the issues in regards to the orbital particles of so many satellites (SpaceX is permitted to launch greater than 11,000 Starlink satellites, way over the present complete of round 2,000 orbit). However he additionally stated that the chosen 500-km altitude and satellite tv for pc design would assist make sure that the constellation cleans itself due to its interplay with the Earth's ambiance.
The launch webcast, embedded under, ought to start roughly 15 minutes previous to launch.
Launch of the Starlink mission.


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Tit for Tatts
An evening of comedy, a chamber composition world premiere, and Tobe Nwigwe's only show of the year.
Are you ready for some really, really big sandcastles?
Fall Arts Guide 2018
The Bayou City's most musician-friendly venue is gone now, but we're still looking forward to these local bands and national tours this fall.
Other than, you know, that Super thing...
Monster burger bash
Houstonia's Burger Bash, anti-fascist theater, and a local art market top the list.
The build took a loyal regular nine months to complete.


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1. Department of Otolaryngology, Head and Neck Surgery, The Second Hospital of Jilin University, Changchun 130041, P. R. China
2. Department of Respiratory Medicine, The Second Hospital of Jilin University, Changchun 130041, P. R. China.
There is accumulating evidence indicating that long non-coding RNA H19 and its mature product miR-675 play essential roles for tumor growth and progression. However, their prognostic value in human head and neck squamous cell carcinoma (HNSCC), particular in laryngeal carcinoma, remains to be elucidated. In this study, we observed that both H19 and miR-675 were significantly overexpressed in a cohort of 65 primary tumor samples and two HNSCC cell lines. Importantly, when paired with patient follow-up data, higher expression of either H19 or miR-675 was significantly correlated with higher risk of patient relapse, and associated with worse overall survival and poor disease-free survival. Knockdown miR-675 caused significant reduction of cell viability, migratory and invasive capabilities. Taken together, these results suggest that the strong correlation of H19 overexpression together with higher miR-675 and lymph node metastases could be useful predictive markers, indicating a potentially therapeutic strategy for HNSCC patients.
Keywords: head and neck squamous cell carcinoma, H19, miR-675, prognostic predictor, proliferation.
As genome-wide expression analysis has been continue to improve, led to increase insights into the molecular events underlying human biology and disease. The recent Encyclopedia of DNA Elements (ENCODE) project has identified and characterized annotated and novel RNAs transcripts that are enriched in either of nucleus and cytosol by RNA-seq . Their results reveal that more than 70% of the human genome to be covered by either processed or primary transcripts, including thousands of non-coding RNAs [1-3].
Long non-coding RNAs (lncRNAs) are a novel class of non-protein coding molecules with more than 200 bases in length. Although lncRNAs share many features of miRNAs, increasing evidence suggests that lncRNAs have essential roles in diverse physiological cellular processes, such as decoys, scaffolds for interacting proteins, chromatin remodeling, post-transcriptional modifications . lncRNAs deregulated expression has recently been implicated in the pathogenesis of many types of cancer. MALAT1 has been reported to be up-regulated in lung  and colorectal cancer . Overexpression of MALAT1 increases cell proliferation, induces cell migration and correlates with tumor metastasis . Other most well established lncRNA, HOTAIR has been observed overexpress in different type of cancers and is positively associated with patients' survival in hepatocellular carcinoma  and colorectal cancers . H19 was first reported up-regulated in bladder carcinoma and has been recognized as a predict marker for early recurrence . Subsequently, in a cohort of 80 archival paraffin embedded hepatic metastases samples, H19 RNA was found overexpression in 64 of 80 (80%) patients . Furthermore, emerging evidence from recent studies has shown that H19 promotes human pancreatic tumors progression  and affects cell proliferation through c-Myc. Overexpression of H19 predicts poor prognosis in gastric cancer and non-small cell lung cancer patients [12, 13]. In contrast to miRNAs, lncRNAs also function as precursors or spongers for miRNAs. As a precursor of miR-675, the tumoriogenesis role of H19 may go through miR-675. In human colon cancer cell lines and primary human colorectal cancer, both H19 and miR-675 have been found to up-regulate and affect colorectal cancer development through suppressing RB .
Head and neck squamous cell carcinoma (HNSCC) is the sixth most common malignant tumor worldwide , and laryngeal carcinoma accounts for 30% to 40% of all malignant head and neck tumors. Despite recent advances in combination with chemotherapy for more advanced diseases, the 5-year overall survival remains poor. As far as several predisposing factors are concerned, including predominantly smoking and alcohol consumption and other possible risk factors such as HPV and EBV infections, work environment and nutrition, the mechanism of carcinogenesis in the HNSCC remains elusive, underlining the importance to better understand the molecular basis and developing novel therapeutic strategies to target this disease. The oncogenic role of H19 in different cancers has been investigated. However, there is no evidence whether H19 and miR-675 are involved in human HNSCC. We therefore evaluate the expression of H19 and miR-675 and its correlation with clinicopathologic features in HNSCC patients.
Quantitative real-time PCR was performed on 62 HNSCC (46 larynx, 14 hypopharynx and 2 oropharynx) and 19 adjacent normal tissues to assess H19 and miR-675 expression. The expression of H19 was significantly higher in the tumor cohorts compared with the adjacent normal tissues (p =0.0063, Fig 1A). Furthermore, when comparing H19 expression in recurrence with non-recurrence patient's group, H19 was significantly higher in the patients that eventually relapsed (p=0.052; Fig 1B).
Prognostic significance of H19 in patients with head neck squamous cell carcinoma. (A). Relative expression of H19 in HNSCC compared with normal tissues. (B). non-recurrence compared with recurrence samples. Kaplan-Meier analysis was performed for overall survival and disease free survival. Patients with high expression of H19 exhibited significantly worse overall survival (C) and shortened disease-free survival (D) than that with low expression of H19 as defined by log-rank test.
Prognostic values of miR-675 in patients with head neck squamous cell carcinoma. (A). Relative expression of miR-675 in HNSCC compared with normal tissues. (B). non-recurrence compared with recurrence samples. Kaplan-Meier analysis was performed for overall survival and disease free survival. Patients with high expression of miR-675 exhibited significantly worse overall survival (C) and shortened disease-free survival (D) than that with low expression of miR-675 as defined by log-rank test.
H19 is a precursor of miR-675 and its function could be at least through derived miR-675. Unsurprisingly, qRT-PCR revealed that miR-675 was positively concordant with H19 expression and significantly overexpressed in the tumors compared with the adjacent normal tissues (p=0.001, Fig 2A).
In order to investigate the association between H19 expression and patient survival, the patients were dichotomized into H19 high (n=37) and low (n=25) groups by using the median expression value of H19. The Kaplan-Meier survival curves showed that patients with higher H19 expression had worse overall survival (OS) and disease-free survival (DFS) compared with those with lower H19 expression by log-rank test (p=0.0048 and p=0.0052, respectively. Fig 1 C and D). Similarly, miR-675 expression level was observed to be correlated significant for overall survival (OS) and disease-free survival (DFS). Higher expression of miR-675 was associated with patient recurrences (p =0.003, Fig 2B) and had worse overall survival (p=0.024) and DFS (p=0.045) than those with low miR-675 expression group (Fig 2C and 2D).
When comparing H19 and miR-675 expression with gender, age, tumor stage, primary tumor location and lymph node metastases, univariate analysis showed that overexpression of miR-675 significantly correlated with patients have higher expression of H19 and lymph node metastases, but not with sex, age and primary tumor location (Table 2 (A)) for both OS and DFS. By using Cox proportional hazard regression model, multivariate analysis revealed that H19 overexpression together with higher miR-675 and lymph node metastases were independent prognostic factors for poor disease-free survival (Table 2 (B); H19; p=0.017; Hazard ratio: 4.11; 95% CI between 1.27 to 13.21). Likewise, H19 was also significantly correlated with overall survival when controlled for the clinical prognostic factors (Table 2 (B); p= 0.0025). The hazard ratio for the effect of H19 on survival when controlled for clinical factors was 14.29, 95% CI was between 3.43 and 59.41.
Analysis of prognostic parameters.
|(p value)||(p value)|
|(A). Univariate analysis|
|Age at surgery (>64 v.s. <=64)||0.78||0.79|
|Primary location (Larynx/others)||0.59||0.37|
|Tumor stage (T1-2/T3-4)||0.057||0.059|
|Relapse v.s. Non-relapse||0.0012||0.0055|
|High H19 (Y/N)||0.0017||0.021|
|High miR-675 (Y/N)||0.021||0.0067|
|(B). Multivariate analysis||OS (HR, 95%CI)||p value||DFS (HR, 95%CI)||p value|
|Lymph node metastases||0.049(0.0060-0.41)||0.0053||0.029(0.0033-0.25)||0.0014|
|High H19||14.29(3.43-59.41)||0.0025||4.11(1.27- 13.21)||0.017|
OS-overall survival; DFS-disease-free survival; HR-Hazard ratio; CI-confidence interval.
Given the evidence that overexpression of H19 and miR-675 were associated with poor clinical outcomes in HNSCC, particularly in laryngeal carcinoma, we further assessed the biological significance of depletion of miR-675 in 2 HNSCC cell lines; Hep-2, a laryngeal carcinoma line and Fadu, a hypopharyngeal carcinoma line. H19 was significantly overexpressed in two examined cancer lines, when compared with immortalized normal human oral epithelial cells (iNOE; Fig 3A). As expected, both cancer cell lines demonstrated higher level of miR-675 expression compared with that of normal (Fig 3B).
To evaluate the effects of miR-675 depletion, cells were transfected with either scramble controls (SC) or anti-miR-675. Expression of miR-675 was significantly reduced by 65% and 70% respectively, at 48 and 72 hours post-transfection (Fig 3C left) in Hep-2 cells. Similarly, in Fadu cells, miR-675 was significantly suppressed by 65% at 48 hours post-transfection and continuously declined at 72 hours (Fig 3C right). Furthermore, transfection with anti-miR-675 led to significantly decreased cell viability in both Hep-2 and Fadu cells, by 30% and 55% respectively, at 48 hours post-transfection compared with controls (Fig 3D). Taken together, these data indicate that overexpressions of H19 and miR-675 are common events in HNSCC, effective suppression of miR-675 warrant further investigation for potential therapeutic applications.
As miR-675 up-regulation was observed to be associated with recurrence in our patients, we performed migration and invasion assays on Hep-2 cells. Compared with their corresponding negative controls, transfection with antimiR-675 significantly reduced migrate capacities of Hep-2 cells by 40% and resulted in reduction of invasion of Hep-2 cells by 30%, as shown in Figure 4.
It has become increasing evidence that lncRNAs play essential roles for human tumour growth and have been found to be key regulators of the complex protein signaling pathways underling carcinogenesis. In the present study, we found that clinically, high expression levels of H19 correlated with patient clinical outcome in our cohort of HNSCC patients. miR-675 expression was positively correlated with H19. Moreover, both high expression of H19 and miR-675 significantly correlated with poor overall survival and disease-free survival. Furthermore, H19 overexpression together with higher miR-675 and lymph node metastases were identified as independent prognostic factors for HNSCC patients. These finding further supported by the evidence of that depletion of miR-675 inhibiting laryngeal and hypopharyngeal cancer cells proliferation, migration and invasion through involvement in cellular processes.
H19 is an imprinted long non-coding RNA gene. Emerging evidence indicates that H19 is frequently overexpressed in the majority of human malignancies. Although it acts as an oncogene in breast , bladder , glioma  and prostate  cancers, some studies also show its controversial role as a tumor suppressor in different tumor types  . In HNSCC, a study by Esteves LI  has shown a significant correlation between H19 expression and tumor recurrence. Consistence with their observation, H19 expression in our cohort demonstrated significant up-regulation in cancer than that in normal. Importantly, the patients with higher H19 expression had worse overall survival and disease-free survival compared with those with lower H19 expression. These results suggest that H19 contributes to oncogenesis of HNSCC.
Down-regulation of miR-675 reduced cell proliferation in squamous cell carcinoma cells. (A & B). Quantitative real-time PCR (qRT-PCR) for H19 and miR-675 expressions in head neck squamous cell carcinoma cells (Hep2 and Fadu) compared with normal epithelial cells (iNOE). (C). Equal amount of Anti-miR-675 (40 nM) or scrambled control (SC) was transfected into either Hep-2 cells or Fadu cells and significantly reduced miR-675 expression in both cells, compared with scrambled control (SC) or transfection reagent alone (Lipo). (D). Cell viability was assessed in both Hep2 and Fadu cells by the MTT assay at 24-72 hours post-transfection. Each datum represents the mean fold change ± SE in triplicates. *p<0.05. **p<0.01.
Depletion of miR-675 reduced cell migration and invasion. Top panel--- Representative images of migration assay depict migratory ability after transfection with antimiR-675 compared to scramble control. Bottom panel--- Representative images of invasive assay depict invasiveness after transfection with antimiR-675 compared to scramble control.
miR-675 is excised from first exon of H19. Both H19 and miR-675 are believed to be involved in tumoriogenesis. It was first described by Tsang WP , in which H19-derived miR-675 regulated primary human colorectal cancer development through down-regulation of tumor suppressor RB. Subsequently, a strong relationship between H19 and miR-675 was confirmed in various studies. H19/miR-675 targeted tumor suppressor Runt Domain Transcription Factor1 and regulated gastric cancer proliferation and development . H19 derived miR-675 promoted glioma cell invasion  and enhanced tumorigenesis and metastasis through downregulating c-CbI/CbI-b in breast cancer cells .
The precise underlying mechanism of H19/miR-675 in tumorigenesis remains unclear. H19 involves in the complex biological process of oncogenesis [2, 17, 26]. Genomic imprinting is an inherited epigenetic phenomenon, loss of imprinting at the IGF2 and H19 loci play a role in the oncogenesis of head and neck carcinoma . H19 expression in many cancers is usually associated with the function of methylation. H19 CBS6 methylation potentially represents a novel clinically relevant epigenetic marker for the recurrence and progression of esophageal squamous cell carcinoma . There are clear evidences show that TGF-β can induce the expression of H19 along with EMT markers, such as Slug, which also contribute to tumor metastasis and dependent on H19. Recent study has demonstrated that H19 functions as a miRNA sponges to promoting EMT in colorectal cancer through activating Wnt signaling pathway . On one hand, H19 overexpression increases the cellular abundance of miR-675  and regulates intestinal epithelial barrier function via miR-675 by interacting with RNA-binding protein HuR . On the other hand, miR-675 up-regulates long noncoding RNA H19 through activating EGR1 in human liver cancer . In our study, miR-675 expression was positively correlated with H19 over-expression in HNSCC. Overexpression of miR-675 together with higher H19 and lymph node metastases were independent prognostic factors for poor disease-free survival. Furthermore, our results were in line with their observation, in that knock- down miR-675 caused significant reduction of cell viability, migratory and invasive capabilities in carcinoma lines, indicating miR-675 involving in HNSCC tumor progression. Nonetheless, further studies in the function of H19/miR-675 axis in the molecular mechanism of HNSCC tumor progression in a large cohort of samples may validate the exploratory findings.
Sixty-five head neck squamous cell carcinoma formalin-fixed and paraffin-embedded samples were collected during the period of 2001-2011, with approval from the Second Hospital of Jilin University. Among of them, sixty-two tissue samples had sufficient tumor cell contains and have been involved in the study. The clinical, demographic and tumor-related characteristics of patients have been descripted in Table 1. The predominant primary sub-site was larynx (46 of 62, or 74.2%). The majority of patients were male (48 or 77.4 %) and median age was 63.8 years. More than half of patients (59.6%) had lymph node metastasis at the diagnosis and 71% patients had locally advanced disease with stage III and IV. The average follow-up time was 3.09 years. During the follow-up period, 25 patients were dead (40.3%) and all of the patients were treated neither with radiotherapy nor with chemotherapy prior to the surgery. Histologically confirmed, adjacent normal tissues from 19 samples were able to obtain either from separate block or from macro-dissected blocks of patients.
All studied tumour (n=65) and adjacent normal (n=19) specimens were assessed on hematoxylin-and-eosin-stained (H&E) slides using standard diagnostic criteria. 62 tumor specimens were confirmed with more than 60% of malignant cells and were selected for the analysis. Based on the H&E review, the samples were macro-dissected. This study was approval from the Second Hospital of Jilin University and all patients have consented for the study.
The human laryngeal cancer cell line, Hep-2, was purchased from the China Center for Type Culture Collection and maintained at 37°C, 5% CO2 in RPMI 1640 complete medium (Gibco, USA) with 10% fetal bovine serum (FBS, Gibco, USA). The human hypopharyngeal squamous carcinoma cell line, Fadu, was purchased from American Type Culture Collection and were maintained in Minimum Essential Medium, supplemented with 10% Fetal Bovine Serum (FBS), 1.5 g/L bicarbonate, and 1 mM pyruvate. The immortalized normal human oral epithelial cells (iNOE) were purchased commercially and cultured in the recommended medium. All cells were maintained in a 37°C incubator with humidified 5% CO2. To knockdown endogenous miR-675 expression, Hep-2 and Fadu cells were transfected using the LipofectAMINE 2000 (Invitrogen) reverse transfection protocol, according to the manufacturer's instructions. AntagomiR-675 and anti-miR scramble negative control 1 (SC; Ambion) were transfected at a final concentration of 40 nmol/L to a 96-well plate.
Clinical Characteristics of Patients.
|Parameter||N=62||(%)||H19 expression*||p value||mir-675 expression*||p value|
* represent the median of H19 or miR-675 expression.
Total RNA containing miRNAs were extracted from either primary tumor tissues or cell lines using RecoverAll Total Nucleic Acid Isolation kit (Ambion) according to the manufacturer's instructions. Briefly, the macrodissected FFPE samples were de-paraffinized with xylene, washed with 100% ethanol. The pellet then was digested by proteinase K. After incubation the pellet in different heating blocks, total RNA and miRNAs were isolated through a filter cartridge and treated with DNase. The samples were then stored in -80ºC until use.
For H19 mRNA expression, the reverse transcription was performed using SuperScript III Reverse Transcriptase (Invitrogen Corp.) according to the manufacturer's recommendations. qRT-PCR analyses were performed using SYBR Green Master Mix (Applied Biosystems) and the ABI PRISM 7900 Sequence Detection System (Applied Biosystems Inc., Foster City, CA, USA). As described previously , the primers used for H19 were: forward: 5′-TACAACCACTGCACTACCTG-3′; reverse: 5′-TGGAATGCTTGAAGGCTGCT-3′. Compared with control specimens, the relative fold change in mRNA expression was calculated using the 2-ΔΔCt method and was normalized to that of an endogenous gene (GAPDH), which was amplified in parallel as the internal control. For miR-675 expression, the RNA was reverse-transcribed with a MultiScribe reverse transcriptase (Applied Biosystems) by using a stem-loop RT primer specifically hybridises with a miR molecule. The RT products were subsequently amplified with sequence-specific primers using Taqman MicroRNA Assay kit (Applied Biosystems) by the Applied Biosystems 7900 HT Real-Time PCR system. RNU48 was amplified in parallel and used as an endogenous control.
The cell proliferation and cytotoxicity of introducing anti-miR-675 were assessed by using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) staining kit (Sigma, USA). Cells were reverse transfected with either SC control, anti-miR-675 or Lipofectamine 2000 and seeded onto 96-well plates (5 x 103 cells/per well). Cell viability was measured at 24, 48 and 72 hours' post-transfection by a microplate reader.
Cell migration and invasion were assessed by using BD BioCoat Matrigel Invasion Chambers and Control Inserts (BD Biosciences). The cells were transfected with either anti-miR-675 or SC (40nM), then seeded on either control inserts (polyethylene terephthalate membrane) or trans-well chambers with Matrigel. Two ml RPMI supplemented with 15% FBS was added to the lower chamber, served as the chemo-attractant. 0.5x104 transfected Fadu cells were re-suspended in RPMI plus 1% FBS, added to the upper chamber (0.5 mls). Twenty hours later, migrating or invading cells attached to the lower surface of the membrane insert were fixed and stained, then counted under a microscope. Relative migration was calculated by comparison with cells transfected with the negative control. The percentage invasion was calculated based on the number of cells which have invaded through the Matrigel insert, divided by the number of cells which have migrated through the control insert.
The differences expression of H19 and miR-675 between each group were evaluated by unpaired, two-tailed Student's t-test. Overall survival (OS) was defined from the time of diagnosis to date of death. Disease-free survival (DFS) was defined from the time of diagnosis to the date of first failure. Survival curves were generated using the Kaplan-Meier method and were compared by means of the log-rank test. In the univariate model, if factors with prognostic significance, they were then further analysed in a multivariate Cox's proportional hazards regression model. A p-value of less than 0.05 was considered to be statistically significant.
In summary, our results indicate that H19 and miR-675 are significantly overexpressed in HNSCC cancer cells and patients. Higher expression of H19 and miR-675 are correlated with poor prognosis. The strong correlation of H19 overexpression together with higher miR-675 and lymph node metastases could be useful predictive markers, indicating a potentially therapeutic strategy for HNSCC patients.
The authors acknowledge the financial support of the Science and Technology R&D program of Jilin Province, China (20150101140JC).
The authors report no conflict of interest in this work.
1. Djebali S, Davis CA, Merkel A, Dobin A, Lassmann T, Mortazavi A. et al. Landscape of transcription in human cells. Nature. 2012;489:101-8
2. Mattick JS. Long noncoding RNAs in cell and developmental biology. Seminars in cell & developmental biology. 2011;22:327
3. Mele M, Ferreira PG, Reverter F, DeLuca DS, Monlong J, Sammeth M. et al. Human genomics. The human transcriptome across tissues and individuals. Science. 2015;348:660-5
4. Cheetham SW, Gruhl F, Mattick JS, Dinger ME. Long noncoding RNAs and the genetics of cancer. British journal of cancer. 2013;108:2419-25
5. Schmidt LH, Spieker T, Koschmieder S, Schaffers S, Humberg J, Jungen D. et al. The long noncoding MALAT-1 RNA indicates a poor prognosis in non-small cell lung cancer and induces migration and tumor growth. Journal of thoracic oncology: official publication of the International Association for the Study of Lung Cancer. 2011;6:1984-92
6. Xu C, Yang M, Tian J, Wang X, Li Z. MALAT-1: a long non-coding RNA and its important 3' end functional motif in colorectal cancer metastasis. International journal of oncology. 2011;39:169-75
7. Yang Z, Zhou L, Wu LM, Lai MC, Xie HY, Zhang F. et al. Overexpression of long non-coding RNA HOTAIR predicts tumor recurrence in hepatocellular carcinoma patients following liver transplantation. Annals of surgical oncology. 2011;18:1243-50
8. Kogo R, Shimamura T, Mimori K, Kawahara K, Imoto S, Sudo T. et al. Long noncoding RNA HOTAIR regulates polycomb-dependent chromatin modification and is associated with poor prognosis in colorectal cancers. Cancer research. 2011;71:6320-6
9. Ariel I, de Groot N, Hochberg A. Imprinted H19 gene expression in embryogenesis and human cancer: the oncofetal connection. American journal of medical genetics. 2000;91:46-50
10. Fellig Y, Ariel I, Ohana P, Schachter P, Sinelnikov I, Birman T. et al. H19 expression in hepatic metastases from a range of human carcinomas. Journal of clinical pathology. 2005;58:1064-8
11. Sorin V, Ohana P, Gallula J, Birman T, Matouk I, Hubert A. et al. H19-promoter-targeted therapy combined with gemcitabine in the treatment of pancreatic cancer. ISRN oncology. 2012;2012:351750
12. Zhang EB, Han L, Yin DD, Kong R, De W, Chen J. c-Myc-induced, long, noncoding H19 affects cell proliferation and predicts a poor prognosis in patients with gastric cancer. Medical oncology. 2014;31:914
13. Zhang E, Li W, Yin D, De W, Zhu L, Sun S. et al. c-Myc-regulated long non-coding RNA H19 indicates a poor prognosis and affects cell proliferation in non-small-cell lung cancer. Tumour biology: the journal of the International Society for Oncodevelopmental Biology and Medicine. 2016;37:4007-15
14. Matouk IJ, Halle D, Gilon M, Hochberg A. The non-coding RNAs of the H19-IGF2 imprinted loci: a focus on biological roles and therapeutic potential in Lung Cancer. Journal of translational medicine. 2015;13:113
15. Tsang WP, Ng EK, Ng SS, Jin H, Yu J, Sung JJ. et al. Oncofetal H19-derived miR-675 regulates tumor suppressor RB in human colorectal cancer. Carcinogenesis. 2010;31:350-8
16. Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA: a cancer journal for clinicians. 2011;61:69-90
17. Matouk IJ, Raveh E, Abu-lail R, Mezan S, Gilon M, Gershtain E. et al. Oncofetal H19 RNA promotes tumor metastasis. Biochimica et biophysica acta. 2014;1843:1414-26
18. Byun HM, Wong HL, Birnstein EA, Wolff EM, Liang G, Yang AS. Examination of IGF2 and H19 loss of imprinting in bladder cancer. Cancer research. 2007;67:10753-8
19. Shi Y, Wang Y, Luan W, Wang P, Tao T, Zhang J. et al. Long non-coding RNA H19 promotes glioma cell invasion by deriving miR-675. PloS one. 2014;9:e86295
20. Ribarska T, Goering W, Droop J, Bastian KM, Ingenwerth M, Schulz WA. Deregulation of an imprinted gene network in prostate cancer. Epigenetics. 2014;9:704-17
21. Cui H, Hedborg F, He L, Nordenskjold A, Sandstedt B, Pfeifer-Ohlsson S. et al. Inactivation of H19, an imprinted and putative tumor repressor gene, is a preneoplastic event during Wilms' tumorigenesis. Cancer research. 1997;57:4469-73
22. Fukuzawa R, Umezawa A, Ochi K, Urano F, Ikeda H, Hata J. High frequency of inactivation of the imprinted H19 gene in "sporadic" hepatoblastoma. International journal of cancer. 1999;82:490-7
23. Esteves LI, Javaroni AC, Nishimoto IN, Magrin J, Squire JA, Kowalski LP. et al. DNA methylation in the CTCF-binding site I and the expression pattern of the H19 gene: does positive expression predict poor prognosis in early stage head and neck carcinomas?. Molecular carcinogenesis. 2005;44:102-10
24. Zhuang M, Gao W, Xu J, Wang P, Shu Y. The long non-coding RNA H19-derived miR-675 modulates human gastric cancer cell proliferation by targeting tumor suppressor RUNX1. Biochemical and biophysical research communications. 2014;448:315-22
25. Vennin C, Spruyt N, Dahmani F, Julien S, Bertucci F, Finetti P. et al. H19 non coding RNA-derived miR-675 enhances tumorigenesis and metastasis of breast cancer cells by downregulating c-Cbl and Cbl-b. Oncotarget. 2015;6:29209-23
26. Nakao M, Sasaki H. Genomic imprinting: significance in development and diseases and the molecular mechanisms. Journal of biochemistry. 1996;120:467-73
27. Rainho CA, Kowalski LP, Rogatto SR. Loss of imprinting and loss of heterozygosity on 11p15.5 in head and neck squamous cell carcinomas. Head & neck. 2001;23:851-9
28. Gao T, He B, Pan Y, Gu L, Chen L, Nie Z. et al. H19 DMR methylation correlates to the progression of esophageal squamous cell carcinoma through IGF2 imprinting pathway. Clinical & translational oncology: official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico. 2014;16:410-7
29. Liang WC, Fu WM, Wong CW, Wang Y, Wang WM, Hu GX. et al. The lncRNA H19 promotes epithelial to mesenchymal transition by functioning as miRNA sponges in colorectal cancer. Oncotarget. 2015;6:22513-25
30. Keniry A, Oxley D, Monnier P, Kyba M, Dandolo L, Smits G. et al. The H19 lincRNA is a developmental reservoir of miR-675 that suppresses growth and Igf1r. Nature cell biology. 2012;14:659-65
31. Zou T, Jaladanki SK, Liu L, Xiao L, Chung HK, Wang JY. et al. H19 Long Noncoding RNA Regulates Intestinal Epithelial Barrier Function via MicroRNA 675 by Interacting with RNA-Binding Protein HuR. Molecular and cellular biology. 2016;36:1332-41
32. Li H, Li J, Jia S, Wu M, An J, Zheng Q. et al. miR675 upregulates long noncoding RNA H19 through activating EGR1 in human liver cancer. Oncotarget. 2015;6:31958-84
Corresponding author: Ke Wang. Department of Respiratory Medicine, The Second Hospital of Jilin University, Changchun 130041, P. R. China. Tel: 13844097779. E-mail: kewang2016com.

domain: literature


text:
'Halloween Do-Over' Brings Joy to Healdsburg After Fire
Written by Steve Mencher
by Matt Villano
When it became clear that Healdsburg was downgrading its evacuation status on Halloween, two local women figured most people would miss out on the typical trick-or-treat experience. The women, Elena Halvorsen and Mandy Carpenter, both have kids in the local public school, and both live in the Parkland Farms neighborhood, the northernmost section of town.
(Image: David Reed and his son, Harlan, enjoy hot food at the Healdsburg Hallloween Do-Over in the Parkland Farms neighborhood on Saturday, November 2. Credit: Matt Villano)
This particular neighborhood — it's where I live, too — is famous for how it goes off on Halloween; during a typical year most residents give out 1,000 pieces of candy in just a few hours. Halvorsen and Carpenter thought about how much their own kids (Halvorsen has three; Carpenter has two) like trick-or-treating in the hood, and organized a trick-or-treating do-over for Saturday, November 2. The moms sent out a flier on Facebook and Healdsburg's Mayor, Dave Hagele (who also lives in the neighborhood) offered to help spread the word. Within a few hours, the word was out all over town: Halloween Do-Over in Parkland Farms on Saturday, everyone was welcome.
Other locals decided to sweeten the pot. Ariel Kelley, who is the CEO of Corazon Healdsburg, contacted the folks at José Andrés' World Central Kitchen, and they worked with another regional nonprofit, Nuestra Comunidad, to come and set up in the park to give out free hot dinners. Local police got on-board. Local firefighters agreed to come. Even the manager of the local Safeway got involved, donating more than 200 pounds of leftover candy for the event. The neighborhood braced for a bigger-than-usual crowd.
Hagele, Carpenter and Halvorsen came up with a specific route for trick-or-treaters to walk. The route included three streets: Arabian Way, Latigo Way, and Saddle Draw. The idea was for trick-or-treaters to make a big loop and end at Barbieri Brothers Park at the end of Saddle Draw, where the gang from WCK and Nuestra Comunidad would set up shop.
The festivities started around 4 p.m. Crowds funneled in by 4:30. Residents set up tables in front of their houses with buckets of candy and wine for friends. Folks came from elsewhere, parked on the street, popped open their trunks tailgate-style and set out buckets of candy of their own. A police officer showed up, parked his cruiser at the entrance to Barbieri Brothers Park and distributed candy as well. When the fire engines arrived, everyone stopped what they were doing and clapped. The truck parked at the end of Saddle Draw, across the street from the entrance to Barbieri Brothers Park, and all the kids and parents came running to thank the firefighters in person.
Volunteers from WCK and Nuestra Comunidad started serving potato-Brussels sprouts-chop meat hash around 5 p.m. They also served salad and reusable bottles of water.
Alma Bowen, executive director of Nuestra Comunidad told me her group brought enough food for 400 people and they ran out.
(Image: Alma Bowen, executive director of Nuestra Comunidad, serves free hot dinner to residents of Healdsburg on Saturday, Nov. 2. Credit: Matt Villano)
Listen: Alma Bowen of Nuestra Comunidad on feeding the neighborhood:
By 6 p.m., the neighborhood was teeming with kids and parents met in the street hugging and pouring each other glasses of wine and beer. Many of these parents were drinking on the street right in front of the aforementioned police officer. There were no open container citations that night; instead, the officer smiled and wished passersby "Happy Halloween" the entire time.
At 6:45 pm, Hagele, an amateur DJ, blasted Halloween music and sponsored a dance party in the street right outside Barbieri Brothers Park. About 150 kids and their parents participated. When the music ended at 7 p.m., Hagele thanked the first responders, thanked the community for participating, and wished everyone a good night. The party wound down around 8 pm, though many neighbors lingered into the evening at afterparties along the trick-or-treat route.
Participants said the event typified the type of community in Healdsburg — a city where everyone looks out for each other and strives to retain the small-town way of life. In all, I estimate about 1,000 people showed up over the course of the night. It was by far the biggest and most memorable Halloween celebration we've ever had.
(Image: The dance party in front of Mayor Dave Hagele's house was a great way for Healdsburg locals to reconnect with each other -- and their community. Credit: Matt Villano)
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domain: sociology