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The Policy implements Division G, Title II, Section 218 of PL 110-161 (Consolidated Appropriations Act, 2008) which states:
SEC. 218. The Director of the National Institutes of Health shall require that all investigators funded by the NIH submit or have submitted for them to the National Library of Medicine’s PubMed Central an electronic version of their final peer-reviewed manuscripts upon acceptance for publication, to be made publicly available no later than 12 months after the official date of publication: Provided, That the NIH shall implement the public access policy in a manner consistent with copyright law.
The Public Access Policy ensures that the public has access to the published results of NIH-funded research.
It requires scientists to submit final peer-reviewed journal manuscripts that arise from NIH funds to the digital archive PubMed Central (http://www.ncbi.nlm.nih.gov/pmc/).
The Policy requires that these final peer-reviewed manuscripts be accessible to the public on PubMed Central to help advance science and improve human health.
More information on the NIH Public Access Policy can be obtained at:
- My NCBI overview video
- My Bibliography overview video
- Linking eRA Commons accounts to My NCBI
- Managing Compliance with the NIH Public Access Policy: video overview and instructions
- Collaborating with PIs and Co-Authors to associate papers with NIH grants, monitor compliance, and simplify reporting
- Using delegates for My Bibliography
- Using My Bibliography to generate the publications section of form PHS 2590
- My NCBI detailed video training excerpted from the January 2013 Webinar
PubMed Central is an archive of full-text biomedical journal papers available online without a fee. Papers on PubMed Central contain links to other scientific databases such as GenBank (http://www.ncbi.nlm.nih.gov/Genbank/) and PubChem (http://pubchem.ncbi.nlm.nih.gov/).
Papers collected under the Public Access Policy are archived on PubMed Central.
Once posted to PubMed Central, results of NIH-funded research become more prominent, integrated and accessible, making it easier for all scientists to pursue NIH's research priority areas competitively.
PubMed Central materials are integrated with large NIH research data bases such as Genbank and PubChem, which helps accelerate scientific discovery.
Clinicians, patients, educators, and students can better reap the benefits of papers arising from NIH funding by accessing them on PubMed Central at no charge.
Finally, the Policy allows NIH to monitor, mine, and develop its portfolio of taxpayer funded research more effectively, and archive its results in perpetuity.
More information about PubMed Central is available at http://www.ncbi.nlm.nih.gov/pmc/about/faq.html.
Manuscript Submission System FAQ's
Research Performance Progress Report FAQ's
MyNCBI Delegates FAQ's
When and How to Comply
These journals post the final published version of all peer-reviewed NIH-funded articles to PubMed Central (PMC) no later than 12 months after publication without author involvement.
List of Method A Journals
These publishers and journals have an agreement with NIH to post individual final published articles in PubMed Central (PMC) on a case-by-case basis. These journals do not automatically post every NIH-funded paper in PMC. Rather, the author can choose to arrange with the journal to post a specific article; this usually involves choosing the journal’s fee-based open access option for publishing that article.
List of Method B Journals
Methods C & D
In Method C, the author or a delegate deposits the final peer-reviewed manuscript into the NIH Manuscript Submission system (NIHMS).
In Method D, the publisher deposits the final peer-reviewed manuscript into the NIHMS.
Regardless of who starts and manages the submission process, authors and awardees are responsible for ensuring that the final, peer-reviewed manuscript is deposited into the NIHMS upon acceptance for publication.
For additional information on submission methods, please visit:
The NIH Manuscript Submission System (NIHMS)
The NIH Manuscript Submission System (NIHMS) is tool for authors and publishers to deposit electronic version of peer-reviewed final manuscripts for inclusion in PubMed Central. Papers in the NIHMS are assigned an identifier (NIHMSID). The NIHMS has a video overview, illustrated submission tutorials and an FAQ.
Avoid predatory and disreputable publishers.
Use the Think, Check, Submit checklist first:
This lecture by Dr Kelly Cobey, from the Ottawa Hospital Research Institute (OHRI), Centre for Journalology, is engaging and informative if you have more time to devote to the subject

domain: library


After making his announcement Wednesday afternoon, Sedarius McConnell, a 3-star defensive end from Georgia, has decided to become an Illinois Fighting Illini football player.
The Illini continue adding players to their roster. While a football schedule and any sense of what a football timeline looks like this fall is very much in question, Lovie Smith and the Illini coaches continue to build the 2021 class.
McConnell is 6-foot-2 and weights 259 pounds. He’s a big, bull-rusher off of the edge and held offers from Power Five programs in Duke, Kansas, Louisville, Minnesota, Pittsburgh, Vanderbilt and West Virginia, in addition to Illinois.
McConnell’s high school, Westlake, is one of the power programs in the entire state of Georgia. Cam Newton played there, as did Adam ‘Pacman’ Jones. More recently, Chance Warmack (OL, Alabama) and AJ Terrell (CB, Clemson) are Westlake HS alums. Three of the top 20 players in the state of Georgia (per Rivals) are all teammates of McConnell — two of them are undecided and the other, wide receiver Dacari Collins, is committed to Clemson.
McConnell joins Prince Green — an Athlete who committed earlier this month — as players from Georgia in Illinois 2021 class.
Check out his junior year highlights here.


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|Dodson And Fogg — The Call |
Dodson And Fogg — The Call
Album release: November 29, 2013
Record Label: Wisdom Twins Records
01. Mystery (3:06)
02. Watch The Skies (2:28)
03. Suddenly (5:37)
04. Late For The Party (4:28)
05. Windmills (3:06)
06. Like A Fool (3:27)
07. I Remember (4:01)
08. Nothing Can Come Between Us No More (2:42)
09. It's Not Time To Leave (3:15)
10. The Call (2:07)
11. Inside (2:20)
12. Running Round In Circles (5:33)
Line-up / Musicians:
• Chris Wade / Vocals, Guitars, Flute, Violin, Keyboards, Percussion
• Ricky Romain / Sitar
• Chloë Herington (Knifeworld) / Sax
• Colin Jones / Trumpet
• Celia Humphris (Trees) / Vocals
• Alia O'Brien (Blood Ceremony) / Flute / Produced by Chris Wade
All Songs written by Chris Wade
Ξ Rocksuker: Dodson and Fogg are both Chris Wade, and this is their/his third album of 2013. No relinquishing quality for quantity, though; preceding LPs Derring Do and Sounds of Day and Night scooped four quails and four and a half quails respectively.
Ξ That is to say, yer man Wade’s a bit of a favourite of ours. Transition is but one compelling aspect of his output, a proclivity and flair for psychedelic rock unfurling more so on each subsequent successor to last year’s mystically folky eponymous debut.
Ξ Celia Humphris of Trees again features, along with members of Knifeworld and Blood Ceremony. Gone is the fluttery flute work of Hawkwind’s Nik Turner, but then these songs don’t really call for it.
Ξ Another bold leap forwards is evidenced straight from the off with the mesmerisingly hallucinatory “Mystery”, followed swiftly by the dimly lit groove of “Watch the Skies”, worthy of Gomez at their best and home to a delightful Pink Floyd style guitar solo.
Suddenly, er, “Suddenly” fires up the acoustic, welcomes in some sitar and weaves a lovely sort of White Album spell, reminding Rocksucker also of Marc Bolan in his more meditative moments.
Ξ The variety is consummately hewn: “Late for the Party” is like Blur in stoner rock mode with extra added ax soloing, “I Remember” a gorgeous spread of laid back ‘n’ jangly major 7th chords, “Nothing Can Come Between Us No More” dippy, trippy and brass-infused like some lost Elephant 6 classic.
Ξ That’s four Dodson & Fogg albums, and four resounding triumphs. Wade deserves an audience wider still than the one he’s been accumulating.
Ξ “Why this band is not gracing the covers of the mainstream music press is a mystery. Perhaps it is only time; discover them now so you can say you knew them back when. To these ears this is Dodson And Fogg’s best thus far. The amount of ideas in this album is staggering and there is more invention in these tracks than most bands manage in an entire career. Your record collection is a sadder place without this album. Trust me.” — Active Listener
Ξ “Since hearing the first Dodson and Fogg album from prolific singer-songwriter and inspired musical inventor, Chris Wade, I’ve feel I’ve participated in a mesmerising adventure. With each successive release the extended inventiveness and undiluted creativity that personifies Dodson and Fogg surpasses the previous offering.And I think it’s only time before a lot more people come to appreciate Dodson and Fogg.” — Folk Words
Ξ "Dodson And Fogg (or multi-talented virtual one man band Chris Wade plus friends) have already established an enviable back catalogue of psychedelic loveliness with their first three albums. In rapid and prolific succession comes their new opus ‘The Call’. (...) To these ears this is Dodson And Fogg’s best thus far. The amount of ideas in this album is staggering and there is more invention in these tracks than most bands manage in an entire career."
Ξ Un album délicieusement rétro mais également remarquablement inventif.
Website: http://wisdomtwinsbooks.weebly.com/dodson-and-fogg.html / Facebook: https://www.facebook.com/pages/Dodson-And-Fogg/282552805161916
|Dodson And Fogg — The Call |
Peter Cat Recording Co.
Tigercats — Pig City
Jesca Hoop — Stonechild
Ø (MIKA VAINIO) — ARC 1
Outer Spaces — Gazing Globe
Lou Reed, John Zorn, Laurie Anderson
ALBUM COVERS X.
Za Zelenou liškou 140 00 Praha 4, CZE

domain: artistic


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Lil Wayne sportin the Play Cloths X Triple Fast Goose
As most of you already know the Time Bomb crew are huge fans of The Clipse's clothing brand Play Cloths. Looks like Lil Wayne is a fan of the Play Cloths X Triple Fat Goose collabo jacket. As soon as we got these jackets in they barely lasted the day before getting completely sold out. One of the lucky buyers was local hip hop talent Boaz who opted to wear it when he opened up for Raekwon on Dec 13th at Mr Smalls.

domain: celebrity


Representative Benjamin A. Gilman defended his district and his incumbency in front of a court-appointed special master in a Manhattan courtroom yesterday, saying that Sullivan, Rockland and Orange Counties were among the state's fastest growing and diverse regions and deserved a representative with seniority.
''While the math of redistricting is strongly in favor of preserving our Hudson Valley district,'' he said, ''I believe my accomplishments should serve as something of a catalyst'' for keeping the district intact.
The math of redistricting, as Mr. Gilman put it, dictates that New York will lose two Congressional seats this year, going to 29 from 31. The question is, which two will go?
Lawmakers have been reluctant to name names, but Mr. Gilman, who has served in Congress since 1972, is considered a likely target, in part because he is a Republican in a fairly liberal district, and in part because he is 79.
Yesterday was the first time that state lawmakers had publicly presented any plan for Congressional districts, and they did so under the threat that as the deadline for candidates to file petitions swiftly approaches, the courts would draft one for them. However, the two plans offered to the special master, Frederick B. Lacey, a former federal judge in New Jersey, were more partisan wish lists than what is likely to be the eventual compromise.
The Senate Republicans proposed doing away with two Democratic incumbents, lumping Eliot L. Engel and Jerrold Nadler, both from New York City, into a single district, and doing the same to Michael R. McNulty and Maurice D. Hinchey in the capital region.
Assembly Democrats offered a plan that would knock out two Republican seats, pitting Amo Houghton and Jack Quinn against each other in the Southern Tier and Sue W. Kelly and Mr. Gilman against each other in the Hudson Valley.
The State Assembly and Senate are expected, however, to work out a deal that will put one Democrat and one Republican out of a job.
A representative for Gov. George E. Pataki said that the governor would not present a plan at the hearing, although last week United States representatives and Congressional aides said that an aide to the governor had circulated a plan that featured two fewer Democratic seats.
Although the Assembly plan appears to pit Mr. Gilman against Ms. Kelly, it puts a chunk of his Rockland County base in a Democratic district, that of Mr. Engel, setting up a potential race between them, said Eileen Larrabee, a spokeswoman for the Assembly Democrats. Representatives are not required to live in their districts, so Mr. Gilman could run against Mr. Engel.
The second set of victims is less clear, but one possibility is that, too, would be a case of letting the voters decide between a Democratic and a Republican incumbent.
But some lawmakers said the two houses might not reach a compromise, particularly if Judge Lacey draws up a plan that either house finds advantageous. He has been ordered to submit a plan by Friday.Continue reading the main story


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HE is recognized by many as America’s greatest poet and Bolton has an enduring fascination with Walt Whitman (1819 -1892).
His close ties to the city and the north of England are a fascinating aspect of the life of the ‘good gray poet’.
On Saturday, the Bolton Museum and Art Gallery will present the UK premiere of ‘Whitman on Walls!’, an American-British collaboration which will launch a series of seven short films featuring more than 50 global actors and 40 local Bolton artists
In 2020, Karin Coonrod, theater director, writer, and lecturer at the Yale School of drama, crafted “Whitman on Walls!” (WoW!), a new hybrid event bringing together living poets and film, described as a drive-in meets poetry slam.
Following performance cancellations caused by the Covid pandemic, Coonrod and Compagnia de’ Colombari, the theater company she founded in 2004, turned her original stage production of Whitman’s poem More Or Less I Am into seven short films.
Lovers of author, Walt Whitman, at Barrow Bridge, Bolton, during poetry reading and march..Images by Steve Holt, Bolton News, Saturday 30th May 2015..
Towards the end of his life, the poet developed a close friendship with a group of admirers at Bolton who became known as the Bolton Whitman Fellowship, The Whitmanites.
A birthday greeting sent by one of the group’s members, JW Wallace, turned into regular transatlantic written correspondence between the poet and the Whitmanites. They celebrated his birthday every year on May 31 — a tradition still carried on in the city today — and some of them visited him at his home in New Jersey.
Lovers of author, Walt Whitman, at Barrow Bridge, Bolton, during poetry reading and walk. Flo Wood’s Reading Book..Images by Steve Holt, Bolton News, Saturday 30th May 2015..
Seven plaques depicting lines from Whitman’s works are in Bolton, Adlington and Rivington.
Recorded over three weeks, with more than 50 artists from around the world, the seven films featured in Whitman on Walls! embody the words of Whitman.
As the work travels around the world, each film will be followed by a local poet who rises and “answers Whitman” in his own words, challenging him or quarreling with him, thus weaving film and live recitation.
Register for the free event via https://www.eventbrite.co.uk/e/wow-whitman-on-walls-tickets-342627186137

domain: news


text:
Using an online casino is a great way to win big while enjoying the comfort of your own home. Online casinos offer hundreds of virtual games to choose from. Many of these games feature impressive visual effects. The most notable of these games include video poker, blackjack, and roulette.
The online gambling industry is growing rapidly and the online casino industry is expected to reach a value of $153 billion in 2030. Most online casinos are regulated, meaning they provide a fair gaming experience. They also offer secure payouts on legitimate wins. A few sites even allow players to play for free. Choosing a reputable casino is the key to a successful gaming session.
The best online casinos have undergone numerous tests to ensure their software is up to snuff. A few of the more sophisticated casinos even offer tutorial videos to help new players get started. Online casinos offer a plethora of games and slots, and some operators even offer hundreds of different slots. Most are easy to play and offer a fun and rewarding gaming experience.
Online casinos offer many free games to get players started. While some games may not offer any payouts, many of them do. There are also free versions of popular games like blackjack, roulette, and video poker. These games are also played on mobile devices, such as the iPad. They also have a nifty nifty feature called the chat feature, which allows players to interact with the dealer.
Taking advantage of the latest technology has never been easier, but you don’t have to spend a fortune to have a fun and rewarding gambling experience. If you have a decent Internet connection, you can play for as little as a few minutes a day.
The best part is that online casinos are the most convenient to play. You can play from the comfort of your home on your laptop or tablet. Some operators offer hundreds of different slot machines, including progressive jackpot slots. The best online casinos also offer games that are designed to be played with your mobile phone. Using an online casino is also a great way to win big and take home a nice little stack of cash.
Although the online casino industry has only been around for a few short years, the most popular casinos have already undergone numerous tests to ensure their software is up to snuff. They also offer some of the most fun and exciting casino games around. The biggest drawback is the cost of play, which is a bit more than playing in a land based casino.
The best online casino games also boast the best jackpots. If you’re lucky enough to win, you might be treated to a vacation to Las Vegas or the Bahamas. Aside from these perks, many of the best online casinos also offer players a large selection of free games, and offer a wide variety of promotions and bonuses. These include a variety of jackpot prizes and free spins.

domain: game


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Stressed out? Eat dark chocolate!
Studies show that eating a small amount of dark chocolate two or three times each week can help lower your blood pressure. Dark chocolate improves blood flow and may help prevent the formation of blood clots. Beyond these health benefits, dark chocolate also contains several chemical compounds that have a positive effect on your mood. Chocolate contains phenylethylamine (PEA), the same chemical your brain creates when you’re falling in love. PEA encourages your brain to release endorphins, so eating dark chocolate will make you feel happier. Dark chocolate also lowers the stress hormones cortisol and catecholamines in the body, thereby reducing your anxiety! (Warning: Diabetics should be cautious about their intake of chocolates, including dark chocolate, to avoid spikes in blood sugar.)
Feeling sluggish? Have a spinach salad!
Impaired blood flow may leave you feeling sluggish or slow to process or recall information. Folate found in spinach may help lower high blood pressure and also relaxes blood vessels, thus maintaining proper blood flow, which consequently re-energizes your body. (Warning: Do not eat excessive amounts of spinach if you are on the blood thinner warfarin (Coumadin) without first speaking with your doctor.
Being cranky? Eat apple slices with peanut butter!
We’re often cranky when we’re tired or hungry. Look to the apple for your healthy complex carbohydrate and the peanut butter for your healthy fat. Combining these powerhouse foods tastes delicious and gives you energy that lasts for hours!
Watch out! Temper that anger with green tea!
Green tea contains theanine, which calms you and helps you maintain clear concentration and focus. (Warning: Do not drink excessive amounts of green tea if you are on the blood thinner warfarin (Coumadin) without first speaking with your doctor.)
Got the blues? Brighten up with whole-grain cereal and milk!
Need a happiness boost? While the source of your sadness could be work- or relationship-related, it also may have something to do with a deficiency of vitamin D in your diet. Vitamin D produces serotonin, which is a “feel-good” hormone that can help you feel calm, relaxed, and happy. If you’re low on vitamin D, you may be affecting your body’s ability to stabilize your mood and reduce feelings of depression. To boost your intake of vitamin D, turn to low-fat fortified milk, fortified cereals, or mushrooms.
Aunt Sally is visiting? Reduce PMS symptoms with an egg sandwich!
In the days before your period, it’s normal for women to begin craving carbohydrates. This is not without good reason: Carbs help your body boost its serotonin levels, in turn helping you improve your mood. However, avoid high-sugar/high-fat carbohydrates (like doughnuts or chips); they can leave you feeling sluggish—or worse, grumpy—after your blood sugar spikes, then drops. Opt for whole grains, such as whole-wheat bread. For extra PMS-zapping strength, pair them with tryptophan-rich protein such as eggs, sunflower seeds, or turkey, which may enhance the release of serotonin. An egg-salad sandwich on whole-grain bread offers the perfect carbohydrate-tryptophan combination.

domain: food


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Remark: Tens of millions of individuals in weak nations are already experiencing losses and damages from local weather impacts. To assist them, we have to break the impasse at UN local weather talks
Lately, the Danish crown princess Mary and minister for growth cooperation Flemming Møller Mortensen met with the Bangladeshi prime minister Sheikh Hasina and overseas minister Abdul Momen in Dhaka to debate local weather change, together with local weather induced loss and injury.
Loss and injury refers to harms and destruction attributable to local weather change impacts that can’t be prevented by mitigation or adaptation. It has been on the agenda of UN negotiations for a few years, however the discussions on the way to take motion has been polarised in a means, which has prevented important progress.
The Danish delegation visited Satkhira district to interact in dialogue with native folks dwelling on the entrance line of local weather change. Damaged embankments, salt water flash floods that wash away homes, salt intrusion in consuming water, low yields because of extreme droughts or water logging after cyclones are more and more a problem.
The district of Satkhira shouldn’t be alone. A current report from DanChurchAid illustrates how poor households in southeast Asia should take loans when their belongings are washed away by flash floods, river erosion or the like.
Many have already migrated to slums in cities, as rebuilding and reconstruction shouldn’t be an choice. In Bangladesh, greater than 50 million individuals are anticipated to be affected by local weather change over the subsequent 40 years, placing strain on pure sources, biodiversity, and native communities.
Philippines inquiry finds polluters answerable for rights violations, urging litigation
The financial impression of local weather associated disasters is large. Research point out that prices associated to loss and injury will rise to between $290 billion and $580 billion a 12 months by 2030.
Different results can’t be counted in cash. Individuals threat dropping tradition, historical past, and even identities, as local weather change turns into a driver for displacement and migration. Whole areas will change into uninhabitable. It’s clear that there’s a drawback that we have to discover joint options to.
Whereas all of us recognise the necessity for pressing motion, a constructive dialogue on the way to handle the difficulty of loss and injury continues to be largely lacking. We have to unlock the deadlocks within the local weather negotiations and keep away from discussions turning into much more polarised.
And in partnerships between governments, civil society and different sectors we have to discover sensible methods to handle the danger of loss and injury dealing with tens of millions of individuals globally. We should work in the direction of making the Glasgow Dialogue on financing preparations for loss and injury lead to one thing concrete and deliverable.
The invoice for loss and injury too typically finally ends up being footed by the general public budgets of the worst affected international locations. The Local weather Weak Discussion board – which Bangladesh now chairs – introduced final month by its V20 ministerial that it’s going to pioneer its personal worldwide loss and injury financing mechanism to direct funds to communities hardest hit by local weather disasters.
The Santiago Community for Loss and Injury may assist catalyse help for loss and injury. We’re all eager to get it up and operating soonest.
This is the reason Denmark hosted the worldwide UN Local weather Change workshop on the operationalisation of the community in Copenhagen final week and it’s why all of us interact actively within the debate.
That is additionally the explanation why Denmark has pledged a contribution to its technical help fund. We’re assured that the workshop helped paved the way in which for making the community operational at Cop27.
Moreover, Denmark will along with Egypt and UK, host a ministerial assembly in regards to the implementation of the Glasgow Local weather Pact this week in Copenhagen. The goal is to establish “deliverables” on the present agreements, together with on loss and injury – and to guarantee that the outcomes from the negotiation rooms are changed into outcomes on the bottom.
We have to get everybody on board if we wish to halt local weather change. We want constructive dialogue and concrete options. And we have to foster new revolutionary partnerships throughout sectors.
Solely by dialogue, cooperation, and sharing of concepts, info, and experiences, we will advance concrete initiatives that may assist folks, communities, and international locations in danger. Our purpose is to show dead-end discussions into cooperation and motion serving to us all to handle local weather induced loss and injury.
AK Abdul Momen is Bangladesh’s overseas minister, Flemming Møller Mortensen is Denmark’s minister for growth cooperation and Birgitte Qvist-Sørensen is common secretary at DanChurchAid.
Supply: Climate Change News

domain: geography


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Gifts and sets for drummers
Would you like to make a drummer or drum fan who is important to you happy? Then take a closer look in this area. Here are some creative gift sets that you can personalize and show your loved ones what they mean to you. So take a look around and let off steam with the designs, whether standard or deluxe, you can't go wrong here.

domain: design


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8) Lorne Park, Mississauga
A residential suburb, Lorne Park is an affluent, family-friendly community with relatively new homes built on expansive property. Roughly 40,000 people call this neighborhood home for many outstanding reasons. First, the schools in Lorne park are rated as some of the best in Mississauga. Therefore, families seeking a top-notch education for their children flock to this suburb. Lorne Park is an outdoorsy area, with residents partaking in outdoor activities in addition to hiking, walking, and bird-watching in the Rattray Marsh Conservation Center. Just 5-minutes down the road, locals thoroughly enjoy The Port Credit village. Here you can sip craft beers, indulge in fine-dining, shop boutique shore, or hire a boat charter. This area is also the site of many locally-hosted events and festivals. Almost half of the households in this neighborhood make $200,000 or more annually. The average home costs $1.5 million and larger 5-6-bedroom homes average over $2 million.


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Background:Silver nanoparticles (AgNPs) are one of the most widely applicable particles whose application is increasing in Nano world daily. Silver nanoparticles have expressed significant advances owing to wide range of applications in the field of bio-medical, sensors, antimicrobials, catalysts, electronics, optical fibers, agricultural, bio-labeling and the other areas. Green synthesis is the safe and easiest method of producing silver nanoparticles. Because of the production of the silver ions, silver nanoparticles are found to have the antibacterial activity.
Objectives:The aim of this study was to investigate antibacterial activity of silver nanoparticles synthesized by using extracts of Hedera helix against Bacillus subtilis and Klebsiella pneumoniae.
Methods:In this experimental study AgNPs were prepared by the reaction of 1mM silver nitrate and extracts of Hedera helix. Antibacterial activity of AgNPs was assessed by using disc diffusion method against Bacillus subtilis and Klebsiella pneumoniae. The AgNPs were characterized by UV-visible (vis) spectrophotometer, particle size analyzer by dynamic light scattering (DLS) method, transmission electron microscopy (TEM).
Results:AgNPs obtained showed significantly higher antimicrobial activities against B. subtilis and K. pneumonia in comparison to both AgNO3 and raw plant extracts.
Conclusions:Biological methods are a good competent for the chemical procedures, which are environment friendly and convenient.
The emergence of nano technology has provided an extensive research in recent years by intersecting with various other branches of science and forming impact on all forms of life . Nanoparticles have expressed significant advances owing to wide range of applications in the field of bio- medical, sensors, antimicrobial, catalysts, and electronics, optical fibers, agricultural, bio-labeling and in the other areas. The synthesis of metal nanoparticles is a more important research branch in nano technology. Chemical procedures are the most widely used methods for synthesis of metallic nanoparticles . With the development of new chemical or physical methods, the concern for environmental contaminations are also heightened as the chemical procedures involved in the synthesis of nano materials generate a large amount of hazardous byproducts. Thus, there is a need for ‘green chemistry’ that includes a clean, nontoxic and environment-friendly method of nanoparticles synthesis .
In the few past years, the potential of various plants for the synthesis of silver nanoparticles (SNPs) was explored. Several plants like rose, banana, hibiscus, geranium leaves, Cinnamomum, Aloe Vera, basil tansy fruits, and sweet pepper were used to synthesize nanoparticles [3-11]. The biological approaches to synthesis of nanoparticles are better than chemical and physical procedures because of low energy and time expenditure. This method requires no toxic solvents and no dangerous material for environment. Green synthesis of nanoparticles is an eco-friendly method and uses natural solvent . Nanoparticles produced by plants are more stable, and the rate of synthesis is faster than that in the case of other organisms. Moreover, the nanoparticles are more various in shape and size in comparison with those produced by other organisms .
The aim of this study was to investigate antibacterial activity of silver nanoparticles synthesized by using extracts of Hedera helix against Bacillus subtilis and Klebsiella pneumoniae.
2.1. Reagent and Materials
All materials used in this experimental study including Nutrient broth media, Meuller- Hinton agar media,and silver nitrate for the synthesis of nanoparticles were purchased from Merck company in Germany. Standard strain of the Bacillus subtilis (ATCC 1715) and Klebsiella pneumoniae (ATCC 1290) were purchased from the Center of Scientific and Industrial Research of Iran.
2.2. Preparation of Hedera helix Extract
Hedera helix leaves were collected from areas that had grown wild. The leaves were washed several times with distilled water to remove the dust particles. The leaves were cut into small pieces and 10 g were boiled in a 500-mL glass beaker along with 50 mL of sterile distilled water for 20 minutes. The extracted was concentrated by evaporation. After boiling, the colour of the aqueous solution changed from watery to yellow colour. The aqueous extract was separated by filtration with Whatman No. 40 filter paper. The leaf extract was stored at room temperature to be used for biosynthesis of silver nanoparticles from silver nitrate.
2.3. Synthesis of Silver Nanoparticles
The source of silver was silver nitrate (1 mM) in distilled water. Silver nitrate solution was prepared and was reduced using Hedera helix extract at room temperature.
The system was stirred and reduction took place rapidly at room temperature under sun light and completed in 15 minutes. During the bio reduction, the chemical reaction takes place resulting in color change in the reactants from pale yellow into dark brown. The appearance of brown color indicates formation of silver nanoparticles. A picture from reaction mixtures and color changing is shown in Figure 1. The formation of AgNPs was furthermore confirmed by spectroscopic analysis, TEM and DLS techniques.
2.4. Characterization of Silver Nanoparticles
2.4.1. UV-Visible Spectroscopy
The formation and completion of silver nanoparticles was characterized by UV-visible spectroscopy using a Double beam spectrophotometer (Perkin Elmer lambda 15). The bio reduction of silver ions in aqueous solution was monitored by UV-VIS spectra of the solution between 360 nm to 600 nm.
2.4.2. TEM Analysis
Transmission electron microscopy (TEM) technique was used to visualize the morphology of the Ag NPs. The 80 kV ultra-high-resolution transmission electron microscope (Zeiss- EM10C). Prior to being loaded into the TEM, the sample was made by grinding using mortar and dropped into a wholly carbon-coated copper grid.
2.4.3. DLS Analysis
To confirm the size distribution of the silver nanoparticles (DLS) (Malvern Instruments, MAL1001767) technique was applied
2.5. Anti-Bacterial Assay of Silver Nanoparticles
The produced colloid of silver nanoparticles was evaluated for antibacterial activity. Disc diffusion method  was used. The discs were soaked with double distilled water, Hedera helix extract, silver nitrate solution and solution containing silver nanoparticles. Then the discs were air dried in sterile condition. Standard strain of the Bacillus subtilis (ATCC 1715) and Klebsiella pneumoniae (ATCC 1290) were selected for the investigating. Pure cultures of bacteria as 0.5 macfarland (108 Cfu/mL) was swabbed on the Muller-Hinton agar plates.
Plates containing media as well as culture were divided in to four equal parts and previously prepareddiscs were placed on each part of the plate. The discs were placed in the following order: disc soaked with double distilled water as negative control, disc soaked with Hedera helix extract, disc soaked with 1 mM silver nitrate solution and disc soaked with solution containing Hedera helix extract mediated synthesized silver nanoparticles. The plates were incubated at 37°C for 24 hours. Then, the maximum zone of inhibition were observed and measured for analysis against each type of test microorganism.
3.1. UV Visible Spectroscopy
In order to confirm the formation of silver nanoparticles, UV Visible Spectroscopy was employed. The UV Visible pattern in Figure 2, shows the characteristic Surface plasma resonance (SPR) of colloidal silver nanoparticles ranges between 300 nm to around 600 nm and exact peak is at 431 nm.
3.2. TEM Analysis
Figure 3, Shows the transmission electron microscopic (TEM) image of colloidal silver nanoparticles. The TEM image indicated the clustered nature of the particles and the surfaces of the aggregates are rough. The particles are more or less spherical in shape-sizes and range from 10 - 30 ± 2 nm in diameter.
3.3. DLS Analysis
Figure 4, presents the histogram of particles size distribution obtained by the DLS analysis for colloidal silver nanoparticles. The particle sizes show a narrow range of distribution and proved an average diameter of 20 - 25 ± 1 nm for silver nanoparticles.
3.4. Antibacterial Activity
Antibacterial property analysis In this study, the antimicrobial property of AgNPs were investigated by growing B. subtilis and K. pneumoniae colonies on Muller-Hinton agar plates supplemented with AgNPs. Results obtained in previous studies also support the antibacterial potential of AgNPs. In the Muller-Hinton agar plate the zone of inhibition was observed around both the disc (AgNO3 and AgNPs) as shown in Figure 5.
In both cases (AgNO3 and AgNPs) inhibition zone for gm+ veB. subtilis and gm - K. pneumonia were equal. But, in comparison to AgNO3 and AgNPs, The zone of bacterial inhibition by AgNPs was more than AgNO3. No zone of inhibition was obtained in case of control and Hedera helix extract.
The bio reduction approach toward the synthesis of silver nanoparticles is also quite eco-friendly when compared to existing chemical and physical methods. In the present study, it is found that Hedera helix extract can also be a good reducing agent for synthesis of silver nanoparticles and its related kinetics would help to synthesis desired size ranges of nanoparticels to suite a variety of applications. The colloidal silver nanoparticles were studied using different characterization techniques such as Uv-vis spectroscopy, TEM and DLS. The resulted of colloidal silver nanoparticles were evaluated for antibacterial activity. The colloid of silver nanoparticles has got enhanced antibacterial activity against Gram-positive (B. subtilis) and Gram-negative (K. pneumonia), bacteria. In 2010 Bylka et al. introduce Hedera helix as medicinal plant and Ivy leave extracts exhibit spasmolytic/antispasmodic, anti-inflammatory, antimicrobial, analgesic, anthelmintic, antitrypanosomial, antileishmanial, antitumor, antimutagenic, moluscocidal, antioxidant and antithrombin activities . In 2001 Hussain et al. used cytotoxic potential of Hedera helix leaves . Due to the development of antibiotic resistance and the outbreak of infectious diseases caused by resistant pathogenic bacteria, the researchers are now searching for new unconventional antibacterial agents. Ansari et al. in 2011 Examined antibacterial effect of silver nanoparticles (synthesis by chemical methods) against Staphylococcus aureus and results showed that AgNPs exhibit bacteriostatic and bactericidal effect towards all clinical isolates . Also, Sadeghi et al. 2011 investigated antibacterial activity of silver nanoparticles (synthesis by chemical methods) against S aureus and E coli and results showed that the antibacterial activity against E coli is lower than that against S aureus, probably because of the difference in cell walls between gram positive and gram negative bacteria . The results of this study are in agreement with our studies.
In 2014 Zarei et al. tested antibacterial effect of silver nanoparticles (synthesis by chemical methods) against four foodborne pathogens (Listeria monocytogenes, Escherichia coli, Salmonella typhimurium and Vibrio parahaemolyticus) that silver nanoparticles showed great antibacterial effectiveness on four important foodborne pathogens .
But in this research silver nanoparticles synthesis by chemical methods that is harmful to the environment. Recently green methods are used for synthesis of Nanoparticles. Mallikarjuna et al. in 2010 synthesis of silver nanoparticles using Ocimum leaf . Mohaseli et al. (2015) synthesis of silver nanoparticles with green method by using Sesame seeds extract .
Also the results of search by Xia in 2010 indicated that the ivy nanoparticles were more efficient in blocking UV light, less toxic to mammalian cells, easily biodegradable, and had a limited potential to penetrate through human skin . Parvu et al. (2015) searched on phenolic compounds and antifungal activity of Hedera helix (Ivy) flowers and fruits. The results showed the antifungal activity of the fresh flower extract was stronger than that of the fresh fruit extract and was compared to that of an antimycotic drug .
Research on antibacteriali effect Hedera helix extract in Iran performed by Hooshyar et al. in 2014 worked on therapeutic effect of Hedera helix alcoholic extract against cutaneous leishmaniasis caused by leishmania major in Balb/c Mice . But, in this study antibacterial activity of silver nanoparticles synthesized by using extracts of Hedera helix searched. This is an interesting report on the antibacterial effect of colloidal silver nanoparticles that was prepared with green synthesis method. It may be investigated as potential candidate for request in futuristic biomedical fields. However our findings could be targeted for the promising potential applications including biosensing devices, and nanoelectronic because of its pollution free and eco –friendly approach. This green synthesis approach shows that the environmentally bengin and renewable leaf extract of Hedera helix can be used as an effective stabilizing as well as reducing agent for the synthesis of silver nanoparticles. Therefore, AgNPs could be a good alternative for cleaning and disinfection of equipment and surfaces in food-related environments.
Bankar A, Joshi B, Kumar AR, Zinjarde S. Banana peel extract mediated novel route for the synthesis of silver nanoparticles. Coll Surfaces Physicochem Engin Aspects. 2010; 368 (1-3) : 58 -63 [DOI]
Baker S, Satish S. Endophytes: toward a vision in synthesis of nanoparticle for future therapeutic agents. Int J Bio-Inorg Hybd Nanomat. 2012; 1 (2) : 67 -77
Kuzma J. Nanotechnology in animal production—Upstream assessment of applications. Livestock Sci. 2010; 130 (1-3) : 14 -24 [DOI]
Sathishkumar M, Sneha K, Won SW, Cho CW, Kim S, Yun YS. Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloids Surf B Biointerfaces. 2009; 73 (2) : 332 -8 [DOI][PubMed]
Ahmad N, Sharma S, Alam MK, Singh VN, Shamsi SF, Mehta BR, et al. Rapid synthesis of silver nanoparticles using dried medicinal plant of basil. Colloids Surf B Biointerfaces. 2010; 81 (1) : 81 -6 [DOI][PubMed]
Sharma NC, Sahi SV, Nath S, Parsons JG, Gardea-Torresdey JL, Pal T. Synthesis of plant-mediated gold nanoparticles and catalytic role of biomatrix-embedded nanomaterials. Environ Sci Technol. 2007; 41 (14) : 5137 -42 [PubMed]
Li S, Shen Y, Xie A, Yu X, Qiu L, Zhang L, et al. Green synthesis of silver nanoparticles using Capsicum annuum L. extract. Green Chem. 2007; 9 (8) : 852 [DOI]
Dubey SP, Lahtinen M, Sillanpää M. Green synthesis and characterizations of silver and gold nanoparticles using leaf extract of Rosa rugosa. Coll Surfaces Physicochem Engin Aspects. 2010; 364 (1-3) : 34 -41 [DOI]
Salam HA, Rajiv P, Kamaraj M, Jagadeeswaran P, Gunalan S, Sivaraj R. Plants: green route for nanoparticle synthesis. Int Res J Biol Sci. 2012; 1 (5) : 85 -90
Karimi J, Mohsenzadeh S. Plant synthesis of silver nanoparticles by Achillea wilhelmsii Pharmaceutical plant. Razi J Med Sci. 2013; 20 (111) : 64 -9
Ramesh P, Rajendran A, Meenakshisundaram M. Green Syntheis of Zinc Oxide Nanoparticles Using Flower Extract Cassia Auriculata. J Nano Sci NanoTechnol. 2014; 2 (1) : 41 -5
Lutsenko Y, Bylka W, Matławska I, Darmohray R. Hedera helix as a medicinal plant. Herba Pol. 2010; 56 (1) : 83 -96
Ibrar M, Ilahi I, Hussain F. The cytotoxic potential of ivy (Hedera helix L.) leaves. Hamdard Med. 2001; 44 (1) : 18 -23
Ansari MA, Khan HM, Khan AA, Malik A, Sultan A, Shahid M, et al. Evaluation of antibacterial activity of silver nanoparticles against MSSA and MRSA on isolates from skin infections. Biol Med. 2011; 3 (2) : 141 -6
Sadeghi B, Jamali M, Kia S, Ghafari S. Synthesis and characterization of silver nanoparticles for antibacterial activity. Int J Nano Dimens. 2010; 1 (2) : 119 -24
Mallikarjuna K, Narasimha G, Dillip GR, Praveen B, Shreedhar B, Lakshmi CS, et al. Green synthesis of silver nanoparticles using Ocimum leaf extract and their characterization. Digest J Nanomaterial Biostructures. 2011; 6 (1) : 181 -6
Mohaseli T, Poorseyedi SH. Green synthesis and characterization of silver nanoparticles using the aqueous extract of sesame seeds. Environ Technol Tarbiat Modarres Univ. 2015; 6 (1) : 1 -6
Malabadi RB, Mulgund GS, Meti NT, Nataraja K, Kumar SV. Antibacterial activity of silver nanoparticles synthesized by using whole plant extracts of Clitoria ternatea. Res Pharm. 2015; 2 (4)
Parvu M, Vlase L, Parvu AE, Rosca-Casian O, Gheldiu AM, Parvu O. Phenolic Compounds and Antifungal Activity of <i>Hedera helix</i> L. (Ivy) Flowers and Fruits. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2015; 43 (1)[DOI]
Hooshyar H, Talari S, Feyzi F. Therapeutic Effect of Hedera helix Alcoholic Extract Against Cutaneous Leishmaniasis Caused by Leishmania major in Balb/c Mice. Jundishapur J Microbiol. 2014; 7 (4)[DOI][PubMed]

domain: biology


‘Electrification’ has been a buzzword in the auto industry over the past few years as carmakers have announced plans for the next decade. Tightening emission regulations have forced them to accelerate solutions which largely use electrification of powertrains, either as hybrids or as fully electric.
Volvo Cars was one of the early companies to make progress in its electrification program, introducing its first model with a hybrid powertrain 7 years ago (in a V60 model). Since then, it has focussed on electrified models while developing a fully electric powertrain which will be unveiled next month.
New model will highlight safety leadership
This all-new powertrain will be used in the XC40 SUV (to be called the XC40 Recharge) and while the company is not talking about the powertrain at this time, it is highlighting the safety of the car’s design and engineering. Despite a fresh set of challenges presented by the absence of an internal combustion engine, Volvo once again lives up to its well known reputation for safety leadership.
“Regardless of what drives a car forward, be it an electric motor or combustion engine, a Volvo must be safe,” said Malin Ekholm, Head of Safety at Volvo Cars. “The fully electric XC40 will be one of the safest cars we have ever built.”
While building on the superior safety standards of the original XC40, the safety engineers had to completely redesign and reinforce the frontal structure to deal with the absence of an engine, meet Volvo Cars’ high safety requirements and help protect the occupants as well as in any other Volvo.
“The fundamentals around safety are the same for this car as for any other Volvo. People are inside, and the car needs to be designed to be safe for them,” said Ekholm.
To help keep passengers safe and the battery intact in the event of a collision, there is a new and unique safety structure for passengers and battery alike in the XC40. The battery is protected by a safety cage which consists of a frame of extruded aluminium and is embedded in the middle of the car’s body structure, creating a built-in crumple zone around the battery. The battery’s placement in the floor of the car also has the benefit of lowering the centre of gravity of the car, for better protection against roll-overs.
Meanwhile, the car’s body structure has not just been reinforced in the front but also at the rear. Here, the electric powertrain has been integrated in the body structure to realise a better distribution of collision forces away from the cabin and reduce the strain on people inside the car.
New Advanced Driver Assistance Systems
In terms of active safety systems, the XC40 Recharge also introduces new technology. It is the first Volvo model equipped with a new Advanced Driver Assistance Systems (ADAS) sensor platform with software developed by Zenuity, the joint venture company owned by Volvo Cars and Veoneer.
The new ADAS platform is a modern, scalable active safety system that consists of an array of radars, cameras and ultrasonic sensors. Because of its scalable nature it can easily be developed further and lays the foundation for the future introduction of autonomous drive technology.
The XC40 Recharge is part of the ongoing plan to have 50% of Volvo Cars’ sales volume to be fully electric by 2025.


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The study of complex congenital cardiac anatomy has previously been limited to 2-dimensional assessment. Now the potential use of 3-dimensional cardiac models could allow for previously unavailable visualization and analysis of anatomy.
While the study of complex congenital cardiac anatomy had long been limited to 2-dimensional (2-D) assessment, the potential use of 3-dimensional (3-D) cardiac models could reveal previously unavailable visualization and analysis of anatomy.
New research presented at the 2014 American Heart Association (AHA) scientific Sessions reported an experimental 3-D printed heart model using plaster or ceramic to potentially help surgeons treat patients born with complicated heart disorders.
Matthew Bramlet, MD, the study’s lead author, assistant professor of pediatric cardiology, and director of the Congenital Heart Disease MRI Program at the University of Illinois College of Medicine, shared, “With 3-D printing, surgeons can make better decisions before they go into the operating room. The more prepared they are, the better decisions they make, and the fewer surprises that they encounter.”
Actual heart models provide a new dimension of understanding beyond the scope of 2-D or even 3-D images.
Researchers chose 3 patients, a 9-month-old girl, 3-year-old boy, and woman in her 20s, all with complex spatial orientation surgical problems. Inexpensive plaster composite material was utilized to create heart models that consisted of 3 components in a short axis orientation, a basal portion, a middle slice containing all four valves, and an apical portion. After studying the models and traditional images, surgeons successfully restored all 3 patients’ severe heart abnormalities.
“You could see that if you make this compromise here, you could fix this problem, and go from a single-ventricle to a 2-ventricle pair. That is the difference, potentially, between a life expectancy of 2 to 3 decades, to 4, 5, or 6, decades,” said Bramlet.
The study concluded low cost, solid opaque cardiac models with intricate intracardiac details improve surgical planning in complicated CHD.
Experts warned 3-D printing was a small study, therefore an emerging technology not yet approved by the Food and Drug Administration.

domain: health


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News 5 Cleveland's John Kosich made national headlines himself on March 8, after asking Vice President Mike Pence tough questions during a satellite interview.
Speaking exclusively with Kosich, Pence defended the GOP's healthcare replacement plan from the concerns raised by Ohio’s two most powerful Republicans, Senator Rob Portman and Governor John Kasich. Both expressed concerns over the plan's impact on the 700,000 Ohioans now insured through ACA’s expansion of Medicaid.
Veteran reporter Kosich also asked the vice president about President Trump's accusations about the Obama administration wiretapping.
National news outlets pick up on Kosich's interview
News 5's interview with Pence comes after Trump tweeted last weekend that former President Obama had tapped his phones at Trump Tower during the election. Trump offered no evidence to back up the accusation.
Pence on the GOP Obamacare replacement plan
In a tweet Tuesday, Kasich said “phasing out Medicaid coverage without a viable alternative is counterproductive and unnecessarily puts at risk our ability to treat the drug addicted, mentally ill and working poor who now have access to a stable source of care,” the governor said.
Vice President Pence told News 5 he spoke with Kasich and Portman about this last week.
"When it comes to Medicaid we know that we can give states just like Ohio even more flexibility to make sure that those programs that exist to serve our most vulnerable can be tailored to the unique needs and challenges of the people of your state,” Pence said.
“As a former governor from your neighboring Indiana I can tell you I'm very confident that this legislation will give Ohio both the resources and the flexibility that your governor, your legislature will need to be able to meet those needs going forward and literally offer our most vulnerable citizens even better coverage.” - Vice President Pence
The price tag of the plan remains unknown as it awaits a final review by the House Budget Office. Pence argued Republian aren't rushing this.
“Well I think we’re moving in a very orderly and timely way,” the Vice President said.
“The Congressional Budget Office numbers will be out a little bit later this week, maybe early next and policy makers will have a different experience than we had six years ago when frankly the Democrats in the Congress and the last administration wrote Obamacare in a back room and literally brought it to the floor of Congress with very little debate.”

domain: politics


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By Morgan Bryce
The first annual I Run Opelika Health Expo and 5K Run/Walk, presented by Laxson Financial Group, LLC and Voya Financial, will be held Aug. 26 from 7-11 a.m. at the Courthouse Square.
Organized by Opelika residents Andre and Greshanda Marcus, the event is focused on raising obesity awareness. According to recent Center for Disease Control statistics, 36 percent of Alabama’s adults are obese, a number that Greshanda said she hopes decreases through events like I Run Opelika.
“I think obesity is a big issue, especially because it runs in my family, and in the African American communities. There is a need to be health conscious and a focus on being healthy inside and out,” Greshanda said. “I think that this event is going to help bring awareness to people struggling with diabetes, high blood pressure and heart disease.”
Local health organizations and nurses from EAMC will be at the expo, offering a gamut of free health screenings, massages, meal planning tips and more. Smooth N’ Groove will have a food truck on site offering healthy drinks and eating options throughout the event.
The run/walk, which starts at 8 a.m., starts and ends in front of the Lee County Courthouse and takes participants through downtown Opelika.
Andre, who works in EAMC’s IT department, said proceeds from the event will donated to the hospital’s employee assistance program, the Cornerstone Society.
After his involvement in a car crash last fall that left him injured and his six-member family with only one vehicle, Andre said Cornerstone intervened and provided hope during their turbulent time.
“One day, Lisa Ruffin, the Cornerstone director, found out about the wreck and called me about it. I came into work a week later, and there were presents for the kids, gift cards for us and another mode of transportation,” Andre said. “After that, I was talking to my wife … we’re both runners, and we both thought it would be a great idea to do a 5K and an expo for Cornerstone and our community.”
Currently, more than 65 runners are signed up for the race. Andre encourages those interested to register before Aug. 14, to insure they receive a moisture-wicking running shirt and SWAG bag, which contains water bottles, coupons to local businesses and more.
“This started out as a small idea with a few partners, and it has grown tremendously over the last couple of months. I think it’s good to have the community come together for a positive event like this,” Andre said. “Recently, there’s been a lot of violence and distractions, and this is a way for us to gather for a positive cause and a positive reason, and we hope it makes a significant impact on this community.”
The expo is free and open to the public, but tickets for the run/walk cost $25 in advance, and $35 on the day of the event. Registration on the day of the event starts at 6:30 a.m. For more information or to sign up for the event, find the event’s Facebook page or visit www.irunopelika.com and www.runsignup.com.


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The Ethylene Oxide (EO) Residuals Analysis procedure is as follows:
Test samples are prepared according to applicable pre-use instructions on the product label, if available, unless sponsor instructions for extraction specify otherwise. There are two methods of product extraction for residual EO analysis which are employed with medical device products, depending on intended device use: exhaustive extraction and simulated-use extraction.
Exhaustive extraction is used if all residual EO is potentially available to the patient or user. In an exhaustive extraction, all residual EO available must be extracted from the device. This means that the device must be extracted in increments (usually 24 hours) until no significant increase in cumulative residue level is detected upon re-extraction or until the amount extracted is less than 10% of what was detected in the first extraction. The usual extraction temperature is 35 – 39°C, unless otherwise requested by the sponsor.
Limited use extraction is used to duplicate patient/user contact with the device. The patient or user would only be exposed to part of the residual EO during normal product use. Unless directed otherwise, the default extraction method will be a water extraction using purified water, USP for 24 +/- 1 hours in a 35 – 39°C incubator.
In any case, the samples should be extracted for a time period and under conditions that equal or exceed the maximum use conditions. The following should be taken into consideration:
- Duration of intended use
- Temperature of the environment in which the product will be used
- Fluid or tissue contact with the device
- Fluid flow across critical surfaces
- Other applicable use or pre-use conditions
Extraction by Submersion
Most devices are extracted by submersion in water. If the devices are very large, a representative portion may be selected. A known amount of purified water is added to the extraction vessel. The ratio of device size to extraction fluid is prepared to maximize extraction efficiency and also allow for adequate detection sensitivity.
Extraction by Fluid Path
Some devices may be extracted by filling the patient or patient fluid contacting portion with water. Components of the device that do not come in contact with the patient or patient fluid may be removed from the device. The device is first accurately weighed and then completely filled with a known amount of purified water.
After extraction, the devices are cooled (to minimize any potential loss upon transfer to sampling vials) and stored at 2 – 8°C until gas chromatographic analysis.
The total extracted residue in mg for EO and ECH is reported in order to satisfy the ANSI/AAMI/ISO requirements. ANSI/AAMI/ISO does not specify any guidelines for EG levels.
Shipping requirements: To ensure that samples received by Nelson Labs are representative of product, please be sure to include complete information on sterilization, aeration, and proper handling of the device. Samples MUST be shipped on dry ice. Dry ice shall remain in the shipping container and be present when the package is opened in the laboratory. The means of shipping the samples will be noted in the final report.
Nelson Labs places EO residual samples on test within 24 hours (weekends excluded). This is contingent upon receipt of a completed Sample Submission Form and completed EO Residuals Extractions form.
If you have additional questions about Ethylene Oxide (EO) Residual test services, or would like to consult with the experts at Nelson Labs, just send us a request or call us at (801) 290-7500.

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LOS ANGELES - You may have seen Dr. Gadget on FOX 11 News often giving away big prizes to our viewers, but this time, he worked with several companies to arrange a $400,000 product donation to the city of Los Angeles.
With the generous contributions, Dr. Gadget was able to get 10,000 bottles of PURE Skin Defense, an all-natural, non-toxic protective spray, to the Salvation Army for distribution on Thursday, May 14th. Members of the Salvation Army and volunteers distributed 2,000 boxes of food at Exposition Park to pre-registered Los Angelenos struggling because of the outbreak. Each box contained an average of 18 meals, a $20 Albertson's gift card, and two bottles of Skin Defense.
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Additionally, more than 300 Crane humidifiers were donated to the Los Angeles Convention Center, which was converted last month into a federal field hospital by the California National Guard. The humidifiers help relieve relieves cough, cold, and flu symptoms and can be converted into personal steam inhalers for patients.


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