text:
Biopharmaceuticals have emerged as the next-gen of therapeutic (large and small) molecules in the pharmaceutical industry
Guest Author: Chad Eichman, Ph.D., Biopharmaceutical Industry Manager
The pharmaceutical industry has a long-standing relationship with small organic molecules. Synthesis gurus of the past, especially the ZenMaster of organic synthesis, Robert Burns Woodward, built a foundation for the industry to create elegant chemical syntheses.
The revolution of organic synthesis transformed the pharmaceutical industry by allowing companies to produce any desired molecule to deploy in nearly all therapeutic areas. Decades of drug development revolved around these small molecules, which vary in complexity, but typically weigh less than 900 Dalton (Da). Aspirin, for example, weighs 180 Da. More complex molecules such as breast cancer therapeutic paclitaxel comes in at 854 Da.
Most small molecule drugs are chemically synthesized, but more complex small molecules, like paclitaxel, are produced recombinantly through cell cultures. Once generated, most of these drugs are packaged into ingestible tablets that are taken orally to distribute throughout the body. The mechanism by which small molecules act is usually through cell penetration, which prompts a desired cellular response. Overall, this classic approach in drug development is a mainstay in the pharmaceutical industry and continues to grow each year.
While the growth of small molecule development continues, biologics, or biopharmaceuticals, have emerged as the next-generation of therapeutic molecules in the pharmaceutical industry. Insulin, the first biologic drug, has been implemented in the treatment of diabetes for nearly a century. Biologics are significantly larger and more complex than their small molecule counterpart. These large molecules range from ~3,000-150,000 Da and their use as drugs require injections instead of the pill format.
Insulin, for example, weighs 5,808 Da, whereas adalimumab (Humira®) weighs 144,190 Da. Because of their large structure and complexity, biologics are not chemically synthesized, but are only recombinantly produced by engineered cells. Biopharmaceuticals are usually derivatives of natural human proteins, which make them ideal for targeted cellular therapy. Unlike small molecule drugs, which penetrate cell membranes including healthy cells, biopharmaceuticals act through external cellular binding to induce the desired cellular response. Moreover, the molecules are capable of site-specific cellular binding, which means they do not interfere with healthy cells, ultimately making them more attractive drugs.
Regardless of the above differences, small molecule drugs dominate the market and account for over 90% of the top 200 prescribed drugs in the U.S. in 2016.1 However, in terms of cost, biopharmaceuticals are drastically more expensive, as demonstrated by Humira®, which generated $18.4 billion in sales in 2017.2 Correlated to the high consumer cost is a significant increase in research and development costs to generate a biopharmaceutical.
One driver for the increased cost of development is because the analytical characterization of biologics is incredibly challenging. Pharmaceutical companies need to characterize biologics in multiple ways, including purity, stability, and function. For purity assays, unlike small molecules, which can be structurally confirmed through high resolution methods like X-ray crystallography and NMR spectroscopy, biologics routinely require a combination of various high and low-resolution techniques to validate structure.
Moreover, during the recombinant production of these large molecules, a multitude of post-translational modifications (PTMs) are generated. These modifications directly influence the function of the protein and are therefore the most critical aspect of protein characterization to consider.
The daunting task of identifying post-translational modifications is made a bit more Zen through advances in chromatography and mass spectrometry.
Scientists rely on classical chromatographic methods such as size exclusion chromatography (SEC), reversed phase (RP), and ion exchange chromatography (IEX), but also on newer separation modes including hydrophilic interaction chromatography (HILIC) and hydrophobic interaction chromatography (HIC).
These methods are implemented in a variety of techniques that are stringed together to complete the full picture of each biologic characterization. Utilizing efficient chromatographic separations in combination with high resolution mass spectrometry (MS) gives scientists an essential element of identification. These high-resolution methods are often utilized to observe even the most minute PTMs.
Both chromatography and mass spectrometry technologies are increasing in resolution and efficiency as research progresses. With the increase in pharmaceutical R&D efforts shifting toward biologics products, analytical companies are also creating novel solutions for biologics characterization. The result of these combined efforts will hopefully lead to a more streamlined and rapid development of biopharmaceutical products for the advancement of human health. Ultimately, a more Zen-infused world.

domain: health


text:
- Manchester City’s match with Borussia Monchengladbach was called off
- Fierce thunderstorms in Manchester forced the city’s Metrolink to close
- The group stage match has been rescheduled for Wednesday at 7.45pm
- City offered the Germans a chance to stay at their luxury training ground
Manchester City offered Borussia Monchengladbach the opportunity to stay at their luxury training ground accommodation after Tuesday night’s game was postponed.
Torrential rain battered the city ahead of the Champions League clash, with referee Bjorn Kuipers calling the fixture off just 20 minutes before kick-off.
City and their German visitors were locked in discussions for over two hours in an attempt to reschedule for Wednesday, the result being a 7:45pm start.
Monchengladbach angled for an early kick-off, understood to have been around 3pm, because of travel preferences after the game. City successfully argued they could not organise sufficient stewarding for the afternoon.
Only a fraction of the 1,600 travelling supporters will be in the North West, given most were booked on flights back to Germany early on Wednesday morning.
The hosts did offer the Monchengladbach squad their plush £200million City Football Academy facilities, where Manuel Pellegrini had his stars staying the night before home matches, as way of recompense for the postponement.
City’s hotel inside their training quarters is rated as four-star, with king-sized beds and en suite bathrooms.
It stands over the road from the Etihad Stadium and would have meant a short trip for the Monchengladbach side after Wednesday’s game.
Pep Guardiola has already made the decision to no longer use that accommodation before games, instead allowing his players to spend time with their families.
A Monchengladbach spokesman said: ‘City offered us the chance to spend the next night in their academy, but that would have made no difference to us because the hotel wasn’t a problem. We wanted to fly back tomorrow [Wednesday].’

domain: news


- Latest available (Revised)
- Original (As made)
This is the original version (as it was originally made). This item of legislation is currently only available in its original format.
25th February 2012
Laid before Parliament
29th February 2012
Coming into force in accordance with article 1(2)
The Secretary of State for Work and Pensions makes the following Order in exercise of the powers conferred by sections 178(1) and (6) and 315(2) and (5) of, and paragraphs 26(7) and 27(2) and (3) of Schedule 7 to, the Pensions Act 2004(1).
In accordance with section 178(5) of that Act, the Secretary of State has reviewed the general level of earnings obtaining in Great Britain during the period of 12 months ending with 31st July 2011. It appears to the Secretary of State that the general level of earnings has increased by 3% during that period.
On a review under section 148(2) of the Social Security Administration Act 1992(2), the Secretary of State concluded that the general level of earnings obtaining in Great Britain exceeds by 2.5% the general level at the end of the period taken into account for the last such review under that section.
Latest Available (revised):The latest available updated version of the legislation incorporating changes made by subsequent legislation and applied by our editorial team. Changes we have not yet applied to the text, can be found in the ‘Changes to Legislation’ area.
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text:
Growing up on a planet engulfed with media, I have lost my self-identity. I have spent the past 18 years of my life being subconsciously taught what is socially acceptable and what is not, and it has transformed me into a faux person. I have watched friends and peers evolve into muted versions of themselves and succumb to the lofty expectations of today.
I cannot remember the last time I made it through a day without worrying about how I am perceived by those around me. Every day, I wake up and am immediately greeted by a sea of faces on the internet that are deemed “perfect.” Throughout the day, I push down urges to dance in the school quad, experiment with outfits that are out of my comfort zone, and say what is truly on my mind to achieve my goal of being accepted by those around me.
In terms of psychology, I have noticed that this feeling I am picking up on goes back to simple ideas around nature versus nurture. In my younger years, I remember being carefree and authentic. Although it is common to be more innocent and optimistic as a child, I do not understand why we feel pressured to completely give up this sensation as we grow up. Each day that I have been further impacted by the ideals and standards around me, I have strayed from the person I was born to be. This is one of the only circumstances in life that I believe nurture is a decision. We can allow ourselves to be impacted by the media, or ignore it and march to the beat of our own drum.
The interesting thing about this phenomenon is that I believe we could all agree that life is much more beautiful with variety. In scientific terms, ecosystems with more biodiversity are more successful. In order to maintain equilibrium, they need plants and animals of all kinds, sizes, and colors.
Rather than accept the fate of being one in a billion of clones, we need to fight this phenomenon by embracing our authentic selves. I have always been drawn towards those who are eccentric and true to their values. Why continue to strive for an idea of flawlessness that is completely unattainable and disingenuous?
I encourage our generation to stop looking to the media and those around us to develop the person we become, but to instead look within ourselves. Our generation should look past the traits that we can not change and instead allow our experiences, environments and passions to shape us.
By Paige Chassman

domain: sociology


text:
AURORA, Colo., Sept. 9, 2019 /PRNewswire/ -- Richmond American Homes of Colorado, Inc., a subsidiary of M.D.C. Holdings, Inc. (NYSE: MDC), is pleased to announce the Grand Opening of Urban Collection at Parkway Point, an exciting new paired home community in Aurora.
Grand Opening Celebration RichmondAmerican.com/ParkwayPoint
Prospective homebuyers and area agents are invited to attend a Grand Opening celebration at Urban Collection at Parkway Point on Saturday, September 14, from 11 a.m. to 2 p.m. Guests will enjoy a complimentary food truck lunch, tour the brand-new Boston and Chicago model homes and learn everything the notable new neighborhood of designer duplexes has to offer.
About Urban Collection at Parkway Point:
- New homes from the low $300s
- Two-story paired floor plans with 2-car garages
- 2 to 3 bedrooms, approx. 1,260 to 1,470 sq. ft.
- Easy access to shopping, dining & recreation
- Close proximity to Buckley AFB, DIA, business hubs, I-70 & E-470
- Complimentary design assistance
Urban Collection at Parkway Point is located at the intersection of E. Alameda Parkway and S. Buckley Road in Aurora. For more information about this and other exceptional Aurora communities, call 303.850.5750 or visit RichmondAmerican.com/Aurora.
About M.D.C. Holdings, Inc.
Operating under the name Richmond American Homes, MDC's homebuilding subsidiaries have built more than 200,000 homes since 1977. Among the nation's largest homebuilders, MDC's subsidiary companies have operations in Arizona, California, Colorado, Florida, Maryland, Nevada, Oregon, Utah, Virginia and Washington. Mortgage lending, plus insurance and title services are offered by the following MDC subsidiaries, respectively: HomeAmerican Mortgage Corporation, American Home Insurance Agency, Inc. and American Home Title and Escrow Company. M.D.C. Holdings, Inc. is traded on the New York Stock Exchange under the symbol "MDC." For more information, visit MDCHoldings.com.
SOURCE M.D.C. Holdings, Inc.


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The main finding of our study is that in mice injected with EMT-6 cells, treatment with a combination of GBE and CTX produced better growth inhibition of tumors compared to PBS-treated mice. GBE or CTX alone did not produce a better inhibition on the growth of EMT-6 cells than PBS. Although the results of the survival analysis did not reach statistical significance, a clear upward shift was seen in the survival curves in the GBE + CTX group compared to the PBS controls. Furthermore, the median latency time comparing the GBE + CTX group to the PBS control group with p = 0.06 suggests that statistical significance likely would have been achieved with a larger sample size. Additional assessment of indicators of ROS and apoptotic pathways supported these results. This finding is important because it might offer a treatment regimen for prevention of tumor recurrence.
As expected, MDA levels were higher and SOD activities lower in PBS-treated mice compared to untreated, control mice because cancers cells produce higher levels of ROS [15, 16]. MDA was lower and SOD activity higher in the GBE group compared to the PBS group, demonstrating the antioxidant effects of GBE. CTX showed opposite effects of GBE on MDA and SOD but adding GBE to CTX caused the mice in the combination group to look similar to the GBE group. These comparisons support previous observations that cancer cells and CTX both increase ROS and provides evidence that GBE can reduce ROS with and without CTX present.
Although there is a balance between the benefits and disadvantages of adjuvant antioxidants in chemotherapy, early co-administration of antioxidants with chemotherapeutic agents might be useful in scavenging ROS. The resulting reduction in ROS signaling pathways may, in early stages, suppress tumor growth. Our results showed that GBE + CTX reduced IκB expression and its phosphorylation above that of CTX alone, resulting in decreased activation of the NF-κB signaling pathway and promoting apoptosis. The pro-death response to GBE + CTX was consistent in the increased expression of FADD, Caspase-8, Cyto-C, Bax and Bax/Bcl-2 ratio showing the highest levels in the PBS group, followed by the GBE, CTX and the GBE + CTX groups. A reverse pattern was observed for expression of Bcl-2. These patterns of expression support the hypothesis that GBE acts to promote apoptosis in the early stage of tumor initiation and enhance the effects of CTX.
Downstream of the PI3-K/Akt pathway is p65 where phosphorylation in the IKK-dependent pathway leads to activation of NF-κB, resulting in the promotion of carcinogenesis [8, 17]. Decreased PI3-K/Akt pathway activity promotes autophagy-mediated cell death [18, 19]. Autophagy is initiated in part by genotoxic stress with an increased expression of p53 [20–22], and our results showed an increase in p53 in the GBE + CTX group compared to the PBS group.
In summary, in our study CTX and GBE was administered to the mice after the tumor cells were transplanted into the mice after only 24 hours, which was the initial stage of tumor growth. There is inconsistency regarding whether antioxidation should be used in tumor treatment . Tumor weight was significantly decreased by CTX + GBE. The mechanisms underlying this change are that GBE + CTX significantly inhibited the activation of NF-kB, upregulated the proapoptotic and P53 genes, and downregulated the PI3K/Akt pathway, which are implicated into the expansion of cancerous cells. It has been reported that ROS will promote the growth of tumor cells by triggering one of the cell growth signaling pathways, NF-kB. GBE is able to remove ROS which can activate NF-kB, as shown in our findings. The expression and phosphorylation of NF-kB were both significantly suppressed in the GBE + CTX group. Moreover, ROS is also able to promote the expansion of cancerous cells by modifying the genes related to apoptosis and to upregulate antiapoptotic genes and downregulate proapoptotic proteins via PI3K/AKT and ERK/MEK pathways . GBE + CTX is also able to increase the expression of genes related to apoptosis Bax, FADD, Caspase-8, and Cyto-C. Furthermore, PI3K/AKT was significantly decreased by GBE + CTX.
The strength of the present study is that we conducted an experiment to investigate whether or not, when the tumor cells was already present in the body, at the early stage of tumor development, chemotherapy and antioxidation would produce a better inhibition on the growth of tumor cells. The findings of our study suggest that CTX alone would not inhibit the growth of EMT-6 cells better than PBS, or GBE, indicating that when tumor cells were present, chemotherapy would not be potent enough to kill the tumor cells inside body. After surgery, antioxidation and chemotherapy might be helpful to fight the recurrence or slow the growth of tumor cells, our findings are in accordance with Thomson’s et al’ .
A weakness of the present study is that the tumor cells were injected into the mice; the procedure may not simulate the development of tumor onset in the real situation. All of the mice had developed palpated tumors on or before day 10 in our experiment. Had we continued the trial period, we might have observed a significant difference in tumor weights between CTX and GBE + CTX treated groups.

domain: medical


-- Page 583 --
Closed Helideck Marking
j. Offshore (VFR) Operating Altitudes for
1. Background. Mid-air collisions constitute
a significant percentage of total fatal offshore
helicopter accidents. A method of reducing this risk
is the use of coordinated VFR cruising altitudes. To
enhance safety through standardized vertical separa-
tion of helicopters when flying in the offshore
environment, it is recommended that helicopter
operators flying in a particular area establish a
cooperatively developed Standard Operating Proce-
dure (SOP) for VFR operating altitudes. An example
of such an SOP is contained in this example.
2. Recommended Practice Example
(a) Field Operations. Without compromis-
ing minimum safe operating altitudes, helicopters
working within an offshore field "constituting a
cluster" should use altitudes not to exceed 500 feet.
(b) En Route Operations
(1) Helicopters operating below 750' AGL
should avoid transitioning through offshore fields.
(2) Helicopters en route to and from
offshore locations, below 3,000 feet, weather
permitting, should use en route altitudes as outlined
in TBL 10-2-1.
Magnetic Heading Altitude
0 to 179 750'
180 359 1250'
(c) Area Agreements. See HSAC Area
Agreement Maps for operating procedures for
onshore high density traffic locations.
Pilots of helicopters operating VFR above 3,000 feet above
the surface should refer to the current Federal Aviation
Regulations (14 CFR Part 91), and paragraph 3-1-4,
Basic VFR Weather Minimums, of the AIM.
(d) Landing Lights. Aircraft landing lights
should be on to enhance aircraft identification:
(1) During takeoff and landings;
(2) In congested helicopter or fixed wing
(3) During reduced visibility; or,
(4) Anytime safety could be enhanced.
k. Offshore Helidecks/Landing Communica-
1. Background. To enhance safety, and pro-
vide appropriate time to prepare for helicopter
operations, the following is recommended when
anticipating a landing on an offshore helideck.
2. Recommended Practices
(a) Before landing on an offshore helideck,
pilots are encouraged to establish communications
with the company owning or operating the helideck
if frequencies exist for that purpose.
(b) When impracticable, or if frequencies do
not exist, pilots or operations personnel should
attempt to contact the company owning or operating
the helideck by telephone. Contact should be made
before the pilot departs home base/point of departure
to advise of intentions and obtain landing permission
Special Operations 10-2-5


How to Troubleshoot the Air Conditioning in Dodge Carsby Contributing WriterUpdated June 12, 2017
The air conditioning in your Dodge car cools the cabin when it is hot outside. The AC system uses a compressor to compress a refrigerant and then pushes that cold air into the passenger cabin with a fan. When this system breaks you may need to replace your air conditioning components. However, before you do that, troubleshoot the problems.
Under The Hood:
- How to Troubleshoot the Air Conditioning in a Dodge Ram
- How to Troubleshoot the Air Conditioning in a 2003 Dodge Truck
- How to Troubleshoot the Air Conditioning in a Dodge Caravan
- How to Troubleshoot the Air Conditioning in a Dodge Durango
- How to Troubleshoot the Air Conditioning in a Dodge Intrepid
Start the truck and adjust the air from low to high and back down again. Listen for the blower motor. The noise should increase when turned higher and decrease when turned lower. The blower motor will be a black cylinder-shape with a white plastic bowl-like piece on top. Unplug the electrical connector and remove it.
Look at the compressor in front of the engine with the belt to make sure it is quiet. Find the compressor by going to the driver's side engine compartment.
Find the two hoses, one small and one larger, coming from the firewall. Follow them back toward the car and you will find the compressor.
Adjust the temperature controls to make sure they are working correctly. The dials should have the proper amount of resistance. If these are not working correctly, it may be a fuse problem.
Locate the fuse box by opening the driver's side door and prying off the panel near the steering wheel with a screwdriver. Remove the fuse with pliers and replace with a fuse of equal wattage to avoid damaging the electrical system.
Check to make sure the dome lights and graphics display are working properly. If they are not, then it may be a control panel problem. Get to the control panel in a Dodge Ram by removing the center instrument bezel. The control panel is directly underneath. Detach the cables and pull it out.
Items you will need
Pull down on the fuse panel cover under the steering column.
Locate the fuse for the air conditioning using the fuse diagram on the underside of the fuse panel.
Use the fuse pullers in the fuse panel to pull the fuse for the air conditioning.
Replace the fuse if the metal strip inside the fuse is broken.
Turn the fan motor to the highest setting. Then turn the air conditioning to the coldest setting. Pay attention to whether you hear the air compressor start. You should hear a low "hum" when the compressor starts. If you don't, the compressor has not started. This means the compressor has failed and you need to replace it. This is confirmed by the lack of cold air coming into the cabin after a few minutes of having the AC on. If the AC compressor starts but there is no cold air coming into the cabin, there may be a leak in the AC system or the system is out of refrigerant. You will need to have the system serviced by a professional mechanic.
Check the controls first. Make sure the air-recirculation button is not selected instead of the air conditioning. This easy step could save you a trip to the dealership or a mechanic.
Start the engine and turn on the air conditioning, adjusting from low to high. Listen for the accompanying sound to increase when turned on high. This is the blower fan. If it does not sound right, the blower motor may need replacing.
Remove the blower fan in a Dodge Caravan by first disconnecting the battery, then waiting two minutes to disable the airbags. Remove the glove compartment, unscrew the blower fan and then unplug the electricity to it. There is only one small cable running to the blower fan which transmits the electricity.
Look at the graphics display on the dashboard. If it is blank, or if the air flow controls do not work properly, the problem is in the control panel.
Get to the control panel by removing the cup holder on the dashboard, then the trim directly below the air controls. Unscrew the two screws that are now visible, followed by the two screws above the radio. Unplug the cables connected to the control panel.
Check the fuses next if the controls and graphic displays are fine. Get to the fuse box in a Dodge Caravan by opening the driver's door and carefully prying off the side panel directly next to the steering wheel.
Remove the fuse with a pair of pliers and replace with one of the replacement fuses provided in the fuse box.
Items you will need
Check the controls first. Make sure the air-recirculation button is not selected instead of the air conditioning. It may seem simple, but could prevent a trip to the dealership or a mechanic.
Listen for the blower motor next. If working properly, the noise of the blower motor will increase when the air is turned to high and decrease when it is turned to low. Find the blower motor in a Dodge Durango by removing the trim panel directly below the glove box. The blower motor is a black piece with a white colander-looking piece attached. Find the retaining tab and push it down while turning the blower motor counter-clockwise to remove.
Look at the compressor in front of the engine with the belt to make sure it is quiet. Find the compressor by going to the driver's side engine compartment. Find the two hoses, one small and one larger, coming from the firewall. Follow them back toward the car and you will find the compressor. Check the condenser which has the fan and sits directly in front of the radiator to see that it is clean.
Adjust the temperature controls on the dashboard. If not working properly, it is probably the control panel. Find the control panel in a Dodge Durango by removing the instrument panel trim. Unscrew one screw in each corner, and then pull the panel toward the back of the car and up at the same time to release tabs holding the panel in place. Pull the control panel out and unsnap the retainers at the ends. Unplug the electrical connection in the back of the control panel.
Check first to make sure that the proper controls are selected, instead of the air-recirculation button. This simple step is sometimes all it takes.
Look first at the graphics display on the dashboard. If it is blank, or if the air flow controls do not work properly, the problem is in the control panel. Disconnect the battery first.
Remove the center bezel from the instrument panel. Locate the heater and air conditioner control in the instrument panel. Unscrew the four screws holding it into place.
Listen for the blower motor to make sure it is working. With the engine on, turn on the air and adjust the pressure. The normal accompanying sound indicates the blower motor is working properly.
Find the blower motor in an Intrepid in the instrument panel. It is black and cylindrical and has a plastic fan attached. Unscrew it and remove it, unplugging the electricity.
Change the fuse if the controls are not working properly. The fuse box in a Dodge Intrepid is on the driver side of the dashboard underneath the panel. Remove a blown fuse with pliers and replace with a 10-amp fuse. Newer models should have replacement fuses.
Items you will need


text:
Monday, 31 March 2014
New Michael Jackson Album Set For Release
A posthumous Michael Jackson album is due to be released in May, five years after the death of the ‘King of Pop’. The album which is set to thrill fans have eight previously unreleased songs.
According to Epic Records, the album, due for release on 13 May, has been titled “XSCAPE”, which is also one of the new tracks mined from Jackson’s archives.
After gaining access to four decades of the late singer’s material, Epic Records, a subsidiary of Sony, chose eight songs that featured completed vocal tracks by Jackson.
The record company then asked producers to ‘contemporize’ the music while “retaining Jackson’s essence and integrity”, a statement by Epic Records read.
Among the producers engaged to give a modern edge to the tracks were global hitmakers Timbaland, Rodney Jerkins, Stargate and John McClain. A deluxe edition will include a selection of the recordings in their original form.
John Branca and John McClain, co-executors of Jackson’s estate said: “Michael was always on the cutting edge and was constantly reaching out to new producers, looking for new sounds. He was always relevant and current. These tracks, in many ways, capture that spirit.”
Epic Records published its first posthumous Jackson album in 2010. “Michael” included nine previously unreleased songs, mostly drawn from the singer’s final recording sessions.


text:
Lege Lines: Texas Temper Tantrum
Freedom Caucus wigs out, “chubs,” stalls, kills every bill in sight
Dear at-risk black mothers, low-income public school students, and victims of online sexual predators: A small but dangerously immature cluster of House Republicans would rather play petty politics than help you.
In retaliation to House leadership supposedly neglecting their priorities this session, the Freedom Caucus – the extremist Tea Party-aligned faction that specializes in legislative disruption – late last week derailed more than 120 local and consent calendar bills, typically uncontroversial legislation meant to speedily pass. The dozen members include Reps. Jonathan Stickland, R-Bedford; Jeff Leach, R-Plano; Matt Rinaldi, R-Irving; Matt Shaheen, R-Plano; Matt Schaefer, R-Tyler; Kyle Biedermann, R-Fredericksburg; Tony Tinderholt, R-Arlington; and Briscoe Cain, R-Deer Park.
"What's happened to us has been personal retribution. It's been personal attacks, petty politics, and this caucus has had enough of it," decried Leach of the ostensibly slow pace of passing anti-choice, gun deregulation, and property tax reform bills, during an impromptu press conference last Thursday, May 11. Without a shred of irony or apparent self-awareness of the hypocrisy of his statement, Leach audaciously then vowed to pursue personal retribution, saying the caucus would "kill the entire local and consent calendar tomorrow, and essentially what we are doing is exactly what they did to our bills."
What ensued next on the House floor was an embarrassing display of petulant, oversized children throwing a tantrum.
With only a few hours left on the clock before the midnight deadline to pass House bills this session, caucus members – notably Stickland and Tinderholt – used the filibuster-like parliamentary maneuver "chubbing" – basically back-and-forth babble – to stall and ultimately kill bills. Sucking up each precious minute as slow, painful punishment to their less extremist GOP counterparts, the caucus' chicanery on the House floor nearly culminated in all-out GOP civil war. The drama was only broken up by an emotional and personal plea from GOP Rep. Drew Springer, R-Muenster, who called on members to halt the time-wasting shenanigans and pass HB 810 to help those like his wife, who suffers from a spinal cord injury, by spurring the use of adult stem cells for patients with terminal illness or chronic disease. "I pray to God every time I go to mass that one day my wife will have the chance to be able to walk," said Springer, by then in tears. While the bill ended up passing 141-0, dozens of others died in what some are tactlessly referring to as the "Mother's Day Massacre."
Among the bills killed by GOP infighting: a measure by Rep. Helen Giddings, D-DeSoto, that would have prevented "school lunch shaming" by not identifying low-income students who run out of money to pay for meals and allowing a two-week grace period for payment. (Thankfully, the Senate tacked the bill onto related legislation and passed it on Tuesday.) HB 2974, by Rep. Tony Dale, R-Cedar Park, would have created a new criminal offense for sexual predators who prey on children online. HB 2365 sought to improve housing for the state's migrant farmworkers, while HB 2107 could have allowed patients with certain illnesses to use medical marijuana if their doctors approve. And, facing an alarming maternal mortality rate, HB 2403 would have required that the state conduct a study on the causes of deaths among pregnant black women, who have the highest risk of maternal death.
But all these important proposals were reduced to rubble in the caucus' wake.
"For the Freedom Caucus, and for the conservative members of this House, it's always, always been about policy, 100 percent of the time," said Leach before the spree. Of course, that's 100% not true. While they pride themselves on being the only true stalwarts of liberty and the only pure conservatives in the House, the Freedom Caucus has proven they are nothing more than vindictive, infantile egomaniacs willing to jeopardize lives for the sake of their primaries.
It can be summed up no better than when five minutes remained on the House clock Thursday night and Stickland, seeking to drown time, requested a personal privilege speech, typically reserved for impassioned calls for deep reflection (like the aforementioned one by Springer). The last-minute tactic is symbolic of a greater flaw: For members of the caucus, the Legislature revolves around their privilege, their egos, and their agendas, the vulnerable be damned.


1, the oil is in a shelf life, when thetime is long, the performance will be reduced and the wear acceleration of themechanical wear parts is accelerated.
2, the engine oil runs through hightemperature in the engine, its physical and chemical properties will change,and the mechanical properties will change.
3, the engine running time is long, the oilimpurities will increase, which will increase the wear speed of the mechanicalparts.
What are the serious consequences of notchanging the oil on time?
1, if the oil is not replaced on time, theoil pressure is too low. The most direct consequence of low oil pressure isthat it will cause half dry friction or dry friction between the various parts,and the engine will produce significant abnormal noise, which will cause burnup.
Burning and tile phenomenon
The following problems in oil pressurereduction will occur when the oil is not replaced on time:
The oil storage is too small, resulting inno oil or oil in the lubricating system.
The oil dirty or sticky cause the oil pumpcan not be effectively inhaled and pumped out of the oil.
The oil dilute or thinning of the engineoil due to high engine temperature will leak out of the engine's frictionpairs.
2, the oil pressure will be too high if theoil is not replaced on time. Too high oil pressure will cause premature damageto the gasoline filter, causing the engine cylinder to accumulate carbon andother failures. It will also reduce the life of the engine.
Carbon deposit in cylinder
When the oil is not replaced on time, thereare several problems that lead to the increase of oil pressure.
The viscosity of the oil is too large (ifthe oil is not changed to the summer oil in the winter).
The oil fluidity decreases with themodification of oil.
Filter cartridge or oil channel blockage.
3, the problem of excessive oil sludge willbe caused by not changing the oil on time. Sludge is usually due to highpressure in the combustion chamber of unburnt gas, acid, moisture, sulfur andnitrogen oxides through the gap between piston ring and cylinder wall into thecrankcase oil, and then metal parts wear the mixed together, then slowlyaccumulate to form sludge.
Too much too much
If the oil is not changed on time, thesludge will increase, and the increase of oil sludge will cause the blockage ofthe filter and oil hole, which will cause the engine lubrication difficulty andaggravate the engine wear.
4, the oil is not replaced on time. Themost serious consequence is to cause the wear of various mechanical parts ofthe engine, such as piston and cylinder. Once the piston and cylinder are introuble, the machine needs to be overhauled, and the overhaul is something thatevery machine owner does not want to see.
Cylinder cylinder wear seriously
The lack of oil, too much oil in the oil,and the decline in the performance of the oil will cause serious wear and tearof the cylinder and piston. If you don't change the oil in time, the aboveproblems will come up basically, so it is very necessary to change the oil ontime.
5, not changing the oil on time will causethe water temperature to be high. As mentioned above, not changing the oil ontime can cause too little oil storage and cause no lubrication or less oil inthe lubrication system. At that time, all parts of the engine will be in semidry or dry friction state.
A large amount of heat is produced by semidry or dry friction, which makes the water temperature rise sharply.
High water temperature
When the water temperature is too high, thecylinder head and the cylinder block can be deformed or damaged. If such afault occurs, the machine is not far from the overhaul.
Remind two points
1, when the oil is replaced, the oil filtermust be replaced at the same time. We must not be able to save money and savemoney and ignore the oil filter core. Why is this?
Because the dirt in the old machine filterwill destroy the quality of the new oil, thus reducing the service life of thenew oil.
2, in the fall you must remember to replacethe winter oil, and in the spring must change the summer oil, unless your oilis the four seasons general oil.
Because the oil is slightly thinner inwinter, in the summer, the oil will be thinner in winter, which is notconducive to the formation of oil film, and will also exacerbate the wear andtear of mechanical components.
In summer, the oil will be thicker afterwinter, which will not be conducive to the flow of oil. It will cause too muchcarbon accumulation in engine block, which will aggravate the wear and tear ofmechanical parts.
Replace the oil on time is very important,if not timely replacement will accelerate wear parts, affect the service life,and even lead to machine overhaul, more serious is in the process of working ifsudden problems will lead to accidents of scuffing of cylinder bore and so on,it is very important to the regular inspection and timely replacement ofmachine oil.


It means there is a problem with either one that comes on.
It is in the exhaust pipe where it comes off of the engine on the passanger side.
It is on because the ECU has detected a problem with the emissions system.
My 04 Silverado is a 2500HD and it is equipped with a class IV hitch.
You can buy the check engine computer and the book comes with it. I know, I bought one. When your check engine light comes on, you plug it in and check the engine and it will give you the code and you look in the book for that code and it gives you the answer.
That would be the 4L60E transmission
The Silverado comes with a lot of different GVW capacities and engines. I think if the Silverado was a half-ton regular cab short bed with the biggest engine package, it would be a very close race for the Camaro.
I have 91 chevy k1500, It does this, Sometimes it will idle a few minutes, then jump to high idle and check engine light comes on, And sometimes its as soon as i start the engine... I was told it was a vacum leak, But im no mechanic
4L60e / 4L65e
Around 3 gallons.


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Thanks to Eyeris who managed to source a pair of extra tickets for Iron Man last night, I got to watch this show before the official release this weekend. Before you ask, the movie is awesome and totally kick ass, you have to watch it when it comes out this weekend. Now lets proceed to a bit of a “review” that might or might not contain spoilers, read at your own risk.
Tony Stark’s first armour
For those who didn’t grow up with American comics (ie: me, I was reading Chinese version of Doraemon and Lau Fu Tze), Iron Man has his super power not via genetic mutation nor being born on some other planet. Instead, somewhat like Bruce Wayne in Batman, Tony Stark is an industrialist who archive superpower-hood via his engineering brilliance. If you haven’t figured it out, he built the freaking armour that allows him to fly, shoot people, carry heavy loads, etc etc.
2nd version in the making
The story doesn’t stray very far from the typical superhero movies, with the introduction of the main character as an ordinary person to the transformation that makes him a superhero to the final fight with the super villain. The difference is, of course, Robert Downy Jr. fits the profile of Tony Stark perfectly, and Gwyneth Paltrow played a brilliant role in being Pepper Potts.
awesome or not?
Then there’s the absolutely awesome CG that blends it perfectly while bringing the awe factor not any less than Transformers. You’ll love the movie, watch it!
the enemy, iron monger

domain: hobby


Time travel? Some hotels and resorts are now trying to ensure you feel good about your visit…before you arrive!
“We want to create a positive image in our guest’s minds so they’re talking about us fondly before they even pack to come here,” says Brian Soma, the director of sales and marketing for J.W. Marriott Ihilani Resort and Spa, in the Ko Olina resort area outside Honolulu. For instance, the website for the 387-room, full-service, beachfront, luxury property includes a click-on for “Ihilani Marketplace,” at which guests can direct the hotel to customize their guest suite to be ready upon arrival.
“It’s a convenience so our vacationers can begin the fun immediately or our busy corporate guests can get right down to business and then maximize their relaxation time.
If they like diet soda, for instance, we can have it waiting for them in their in-room refrigerator. They can pre-order a certain type of wine. There are plenty of choices on the ‘Ihilani Marketplace,’” says Soma. And don’t expect the typical, gauging of the overpriced mini-bar of some resorts at Ihilani, according to Soma, who says, despite Ihilani being an upscale resort, six-packs of diet soda can be ordered for as little as $6.00; and bottles of wine as low as $15.00. And once on-property, don’t miss the $6 Mai Tai’s during “happy hour” from 9-midnight overlooking the pool. In terms of value, pre-arrange “SpeediShuttle,” which can get you from Honolulu Airport to Ko Olina for only $15. “A speedy check-in process upon arrival is vital, too,” adds Soma. “Guests walk in with their luggage on wheels and are ready to roll to their rooms.”
With a beach showcasing blazing ocean sunsets, Ko Olina hosts 6,000 weddings a year, according to Tim Deegan, Ko Olina’s director of marketing, and newlyweds have the gorgeous keepsake photos to prove it. The Ihilani online marketplace recognizes that by offering romantic gifts for newlyweds and honeymooners. “You can even pre-order a surprise of rose petals on the bed to spell out ‘I Love You,’” says Soma. You can also reserve, or send the gift of a round at the adjacent Ko Olina Golf Club, which hosts an LPGA tournament; a traditional Hawaiian Lomi Lomi massage and Thalasso sea water treatment in the spa; or a meal in one of five restaurants, including Azul, the dazzlingly delicious Italian, open-air restaurant perched above Ihilani’s grotto filled with Hammerhead and Galapagos sharks and Spotted Eagle Rays, which you can hand-feed in the water daily with the supervision of a marine biologist daily. (They won’t get the gnocchi and Chianti you had, though.) You can also book a thrilling snorkel cruise from the resort’s marina at OceanJoyCruises.com, which guarantees you’ll see dolphins.
Ihilani, next door to Disney’s Aulani Resort and the Marriott Vacation Club (in addition to other lodging
options) has hosted President Barack Obama and world leaders during the APEC Summit, and the NFL’s best players call the elegant Ihilani home for a week during the annual Pro Bowl in January. See www.KoOlina.com or www.ihilani.com for more information.
Michigan-based travel writer Michael Patrick Shiels may be contacted at firstname.lastname@example.org or via www.TravelTattler.com. His weekday morning radio show airs on 92.1 FM in Lansing.


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Commodity Supplemental Food Program (CSFP)
The Commodity and Supplemental Food Program provides commodity foods and nutrition education to adults age sixty and over. This program is based on income eligibility.
Click on the link for map and directions to the Commodity Foods Warehouse.
1417 Murfreesboro Road
Nashville, TN 37217
Monday - Friday 8:00am - 4:30pm

domain: economics


text:
Posted by: marek
So far I was thinking that homeopathy is the safest way of treatment, which is not making any harm. But few days ago I have found this: it was written that giving continuously a remedy in potency too high for the body (higher than the level of central disturbance) can lead to the death of a patient.
Is it possible that any remedy can does it?
Death per se is unlikely with the homeopathic remedies. The maximum that can happen is that the person taking an non-indicated remedy for too long ends up proving the remedy, that is produces some symptoms of the remedy. But they should be temporary and reversible in most cases.
Dr. Manish Bhatia


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Pet water fountain are steadily starting to trade the usage of water bowls for pets and it is as a result of water fountains are additional handy for the pets to drink from. This is the primary alternative of the highest 10 greatest dog water fountains in 2020 as it features a 168 Ounce capability and is appropriate for dogs of various size. It has a free-falling stream of water that entices extra drinking out of your canines. It also dog fountain contains carbon water filters which can be replaceable which get rid of bad smells and tastes and likewise keeps water contemporary. The built-in reservoir will increase the capacity of the fountain thus requiring less refilling. The water degree ought to stay above minimal line to stop the motor from humming loudly. It’s made of prime-shelf, dishwasher secure, and BPA Free plastic.
At 10.5 x 8.seventy five x 5.seventy five” in measurement, the Petsafe Drinkwell Ceramic Fountain can hold 70oz. of water (eight.seventy five cups) at a time. The best part about utilizing a ceramic material: it retains your canine’s drinking water cool. It particularly has an upper and a decrease water fountain for dogs stage, so multiple pets of various heights can simply drink on the same time. Plus, the dual free-falling streams will further add oxygen and encourage your canine to maintain consuming (not to point out, the trickling water sounds pretty stress-free too!).
As common, we all the time love the merchandise which might be long-lasting and who are we kidding price your cash. Furthermore, it should water fountain for dogs be fully functional. Out of the three merchandise that we examined out – PetSafe DrinkWell Original Cat and Canine Water Fountain turned out to be our pet’s best friend.
Thinking About Elements For Dog Water Fountain
The consuming cup is pretty small. It works high quality for canine with a smaller muzzle. Finally, a high quality water fountain should dog fountain be sturdy and final for a very long time. A good-quality fountain will supply recent water to your dog for years earlier than it must be replaced.
If you’re searching for a fountain that stands out then the Catit flower fountain is the one for you! That includes a white flower with a yellow stigma from which the water flows over the white petals into the green water bowl, this drinking fountain is as vibrant as it is artistic.
Cats, like us human beings instinctively associate running water with safety and cleanliness. Drinking fountains for cats are outfitted with charcoal and foam filters that rid the water of smells and tastes that are disagreeable as well as scoop up any hair or fur that falls in.
These are nice advantages on your canine, but what about you? Most dog water fountains hold a lot more water than a canine bowl. Meaning you don’t need to fill them as usually, and it is less possible your canine dog water fountain will run out of water. The water flow is probably not as sturdy when you have got the filter in the fountain.
If clipped from inside, it is simple to your canine to knock it off and chew the plastic clips. A dog fountain keeps the water cool by solely releasing small parts of water at a time. Cool water is just https://smartdogstuff.com/dog-water-fountains/ not solely more nice to drink, but in addition offers a much-needed reduction in the course of the hottest hours.

domain: pet


text:
World Leadership Federation,symbolizing excellence in leadership capabilities across industry domains globally, has announced and congratulated the... ...http://businesswireindia.com/news/news-details/world-leadership-federation-announces-its-winners-india-leadership-awards-2016/51240
AGRA: With an eye on a world record and an entry into Guinness, Uttar Pradesh government on the eve of state's second International Bird Festival,... ...http://timesofindia.indiatimes.com/city/agra/UP-eyes-Guinness-with-bird-watching-activity/articleshow/55768034.cms
Meeting discusses Dos and Dont's for party candidates on accepting election donations. In a bid to introduce more transparency in its fund-raising... ...http://indianexpress.com/article/india/punjab-assembly-polls-2017-election-funds-donor-pan-must-if-you-take-over-rs-20000-aap-to-cadres-4407594/
Indian sportswomen PV Sindhu, Sakshi Malik, Dipa Karmakar and Vinesh Phogat made it to the list of top newsmakers of 2016. Over the past few years,... ...http://indianexpress.com/article/sports/sport-others/pv-sindhu-sakshi-malik-top-newsmakers-of-2016-in-yahoo-review-4406894/
New Delhi [India], Dec. 02 (ANI): To support the national agenda set by the Prime Minister Narendra Modi following demonetisation, Fortis... ...https://in.news.yahoo.com/fortis-charitable-foundation-extends-helping-hand-india-queues-082801697.html
The Law Office of Pardeep S. Grewal...


SCAO Informatica uses a custom variant of the Neuron S103 controller to measure the efficiency of industrial machinery. The device performs forecasts and in-depth analysis or estimates consumption.
SCAO Informatica Srl, an Italian company and software house with an international presence, specializes in software solutions for industrial management. Its products effectively improve processes, optimize costs and reduce machine control problems. It focuses on providing Industry 4.0 software solutions and, thanks to its projects, has become a recognized expert in IT solutions for industrial enterprises. Its comprehensive portfolio of products and services supports businesses in improving their efficiency, productivity and overall competitiveness.
ProblemSCAO's hardware solution had to be designed to measure and monitor CO2 emissions and energy consumption in the factory or on specific work orders to predict and subsequently reduce relative costs.
Furthermore, the device had to operate with CNC and PNC-equipped machine tools as well as with older machines where only electrical signals are collected.
The aim was to create a product designed for:
- Measuring the efficiency of industrial machines
- Monitoring the costs of work processes
- Comparing processes with work plans
- Monitoring machine downtime and maintenance
SolutionAll requirements are met by the eMachina Box controller, which is a rebranded variant of the Neuron S103 controller and represents an innovative solution in the field of the Industrial Internet of Things (IIoT).
The eMachina Box (Unipi Neuron S103) contains the following features:
- Digital inputs
- Digital outputs
- Analog input
- Analog output
- RS485 serial interface
- 1-Wire bus
- Product branding
With the eMachina Box, it is possible to connect, for example, industrial machines equipped with various devices or sensors such as scales, counters and photocells. The product can measure the efficiency of machine tools, presses, welding machines, CNC and more.
The information is collected and stored in the Microsoft Azure cloud. The eMachina Box device communicates with the eMachina MES software and passes it all the information it receives from the connected machines.
The eMachina solution performs forecasts, estimates consumption, monitors the amount of CO2 produced, performs in-depth analyses or, for example, monitors machine utilisation or maintenance.
- Allows real-time monitoring of all work centres
- Meets Industry 4.0 requirements
- With the solution, industrial companies can understand factory bottlenecks and increase productivity and efficiency


text: listy-leagueoflegends.eu'd listing of suppliers manufactures and distributors of gaming machines and related products.Auto Roulette & Electronic · Distributors · Components. Slot Machine categorized casino and gaming industry suppliers and vendors including Slot Machine categorized news articles, press releases, and tweets. An economic slowdown may crimp casino profits, but it shouldn't have much impact on gaming machine makers. Occasionally you would find free spins, or limited bonus rounds. Slot machine providers create and manufacture slot machine games for casinos throughout the world. These are some of the most popular variants of the Rainbow Riches saga:. Both IGT and Scientific Games saw the wisdom in letting the brand continue under its own name, which it still does. Yet there are also many companies who have evolved since the boom of the internet, who exclusively develop games for online casinos. Where do slot machines come from? Home Top Sites United States United Kingdom European Canadian Australian Safest High Stakes How We Rank Online Casino Top Online Casinos Introduction to Online Casinos Casino Gambling for Beginners Guide to Casino Games Real Money Casinos Live Dealer Casinos Casino Strategy Sports Betting Football Betting Guide Introduction to Sports Betting Types of Sports Betting Online Sports Betting Odds Converter Best Sports Betting Sites Football Betting Sites Horse Racing Basics of Horse Racing Betting Online Horse Racing Betting Best Horse Racing Betting Sites eSports Betting eSports Betting Sites eSports Tournaments and Leagues Popular eSports Games CSGO Betting Sites League of Legends Betting Sites Reviews Bovada SportsBetting. Plasma euro casino gratis spielen machines glass egraving machine channel letter bending machine. If you fancy playing some of these popular erotische spiele kostenlos without even leaving the comfort of your home, you champions league wett tipps find a wide selection franzosisches roulette gewinn Leo Vegas. Every month more than 2 million unique visitors from all over the world use the platform to search for products, services and suppliers. Simferopol, Ukraine IgroService Corporation is a leader in production of modern gambling and entertainment equipment having certificates of Game play was relatively bet365 app ios, and there were few extra features. These best casino online tend to have a strong reputation throughout the industry, and many of them stargames 5 euro proven to be nearly as successful on the Internet as they previously were in brick-and-mortar locations. Every casino koln roulette machine and game that Apollo Games creates focuses on the experience.

domain: entertainment


text:
Myeloid checkpoint CD47/SIRPα downregulates innate immune surveillance, but CD47 expression on normal cells, especially RBCs, poses a crucial challenge to delivery of CD47-targeted ICB to the TME. Chen et al. showed a bispecific antibody with high affinity for PD-L1 and moderate affinity for CD47 to be well tolerated, TME selective, tumor suppressive, a promoter of progenitor CD8+ differentiation, and a robust activator of both CD8+ effector and innate immune response pathways within the TME. A bispecific antibody therapeutic candidate was well tolerated in NHPs and drove activation of both adaptive and innate immune pathways.
Contributed by Margot O’Toole
Background: T cell checkpoint immunotherapies have shown promising results in the clinic, but most patients remain non-responsive. CD47-signal regulatory protein alpha (SIRPα) myeloid checkpoint blockade has shown early clinical activity in hematologic malignancies. However, CD47 expression on peripheral blood limits αCD47 antibody selectivity and thus efficacy in solid tumors.
Methods: To improve the antibody selectivity and therapeutic window, we developed a novel affinity-tuned bispecific antibody targeting CD47 and programmed death-ligand 1 (PD-L1) to antagonize both innate and adaptive immune checkpoint pathways. This PD-L1-targeted CD47 bispecific antibody was designed with potent affinity for PD-L1 and moderate affinity for CD47 to achieve preferential binding on tumor and myeloid cells expressing PD-L1 in the tumor microenvironment (TME).
Results: The antibody design reduced binding on red blood cells and enhanced selectivity to the TME, improving the therapeutic window compared with αCD47 and its combination with αPD-L1 in syngeneic tumor models. Mechanistically, both myeloid and T cells were activated and contributed to antitumor activity of αCD47/PD-L1 bispecific antibody. Distinct from αCD47 and αPD-L1 monotherapies or combination therapies, single-cell RNA sequencing (scRNA-seq) and gene expression analysis revealed that the bispecific treatment resulted in unique innate activation, including pattern recognition receptor-mediated induction of type I interferon pathways and antigen presentation in dendritic cells and macrophage populations. Furthermore, treatment increased the Tcf7+ stem-like progenitor CD8 T cell population in the TME and promoted its differentiation to an effector-like state. Consistent with mouse data, the compounds were well tolerated and demonstrated robust myeloid and T cell activation in non-human primates (NHPs). Notably, RNA-seq analysis in NHPs provided evidence that the innate activation was mainly contributed by CD47-SIRPα but not PD-L1-PD-1 blockade from the bispecific antibody.
Conclusion: These findings provide novel mechanistic insights into how myeloid and T cells can be uniquely modulated by the dual innate and adaptive checkpoint antibody and demonstrate its potential in clinical development (NCT04881045) to improve patient outcomes over current PD-(L)1 and CD47-targeted therapies.

domain: literature


Russia's defence minister has hit out after Nato applied pressure on Spain to deny port access to Russian warships en route to Syria.
Sergei Shoigu said the refusal has not affected the mission of the Russian aircraft carrier group, adding that it has all the necessary supplies.
The aircraft carrier Admiral Kuznetsov and its escorting vessels are now in the Mediterranean, heading to Syria's coast.
Russia had sought permission for some of the ships to call at Spain's North African enclave of Ceuta last week, but later withdrew its request as Nato allies piled pressure on Madrid over concerns that the ships could be used to ramp up air attacks in Syria.
Mr Shoigu said: "It's time for our western partners to determine whom they are fighting: terrorists or Russia."


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You just need to do some simple medical tests to reflect your medical status and then proceed according to the clinic’s instructions and recommendations.
The prospective parent does not have to be considered undetectable, but even if he is, the sperm is still required to undergo examination. This is due to the fact that even if the viral load in the blood is negligible this does not necessarily mean that the sperm is not contagious.
The sperm washing test is not the only thing that this process includes. After collecting 3 semen samples within one week, a special laboratory tests the sperm and selects the cryopreserved sperm with an undetectable viral burden [fewer than 200 copies of HIV RNA and fewer than 10 infected cells per ml of semen]
There is only one FDA approved clinic in the U.S. (Bedford Research Foundation Clinical Laboratory in Massachusetts) which conducts the semen testing process. The prospective parent is required to visit the clinic regularly over an 8 day period. After approximately one month the test is complete and the selected sperm is sent to the FCLV clinic, with which we work, for the IVF process. The FCLV Clinic is ranked third among all U.S. clinics in terms of egg donation success rates. We have been working for over 15 years with this laboratory and led over 100 intended parents living with HIV to become happy parents with strong healthy kids.
Allow yourself 8 days in Boston for the 3 semen samples the clinic needs and about a month for the clinic to complete all tests and transfer the seed to the clinic where the IVF procedure will take place.
Baby Bloom has extensive experience with intended parents living with HIV in collaboration with the Bedford laboratory clinic that does the IVF process. All such processes ensure total medical confidentiality.
The first baby born to parents living with HIV after undergoing the abovementioned test was born in 1999 HIV free. Since then and until today, not a single recorded case exists in which a child who was born under such circumstance contracted HIV as a result of the process.


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Causes for the American Revolution: From Baby Steps to Strides
"This is not a rebellion, this is a mass awakening." The Catholic priest Don Andrea Gallo gave these words in reference to the protests of July 2001; however, his words also describe America in the 1700's. A mass awakening is exactly what incited a soon to come rebellion from Great Britain. One such awakening, appropriately named the Enlightenment, was what truly initiated the revolution.
If the reason behind the American Revolution is considered a puzzle, the Enlightenment opened a box to reveal the pieces. The Enlightenment was characterized by the belief that the world was a logical, or…
- Causes for the American Revolution: From Baby Steps to Strides
- Events Leading to the American Revolution
- Mercantilism, the Great Awakening, and the French and Indian War, laid sufficient soil to the American revolution
E-pasta adrese, uz kuru nosūtīt darba saiti:
Saite uz darbu:

domain: history


text:
The best build for the TAQ-56 (Scar) in MW2
The TAQ-56 is one of the best Assault Rifles in MW2 but what’s the best setup for it?
The TAQ-56 is one of the strongest Assault Rifle weapons in Call of Duty: Modern Warfare 2, but what is the best build for the gun in MW2?
Better known to franchise fans as the SCAR, the TAQ-56 is a fantastic option for mid-to-long range gunfights. If you are expecting a close quarters-style fight, this isn't the gun for you. Fans know it as the SCAR because it is modelled after the real-life FN SCAR-L.
How to unlock the TAQ-56
Unlocking the TAQ-56 is incredibly simple. Unlike some of the other weapons in MW2, you can get access to the TAQ-56 by simply increasing your "Military Rank" in-game. This is your account's overall level. It is a base weapon and not part of a weapon tree.
The TAQ-56 is unlocked at Level 19. You will have to play with other weapons before being able to use this one. Although, if you played the MW2 beta and levelled all the way to level 30, you will have access to the Frontal Impact blueprint.
The blueprint comes with some pretty decent attachments for the gun and will let you level up whilst using it.
The best build for the TAQ-56
The new customisation system in MW2 makes it quite difficult to nail down one set of attachments that is better than all of the others.
Instead of the 10 attachments that you could put on your gun in Vangaurd, you can now only use five. That makes deciding what is important to have on your weapon all the more important.
Weapon tuning is also available for every weapon in the game and can be unlocked by levelling up each individual weapon to its max rank in the game. It adds another level to the complexity of how to optimise each weapon to individual playstyles.
Most come down to personal preference but these are the best that we've used so far.
The best TAQ-56 build in MW2:
- Rear Grip: Demo CleanShot Grip
- Underbarrel: FTaC Ripper 56
- Muzzle: Komodo Heavy
- Barrel: 14.5" Tundra Pro
- Optic: Cronen Mini Pro
Our attachment choices aim to minimise recoil and add to the damage range of the weapon. It makes it a force to be reckoned with in distance gunfights.
The TAQ-56 is a very versatile weapon and can allow for a slower or faster pacing depending on which attachments or tunings you want to use on your gun. For us, we've left the tuning completely untouched on our TAQ-56 build, keeping the balance of the base weapon.
Modern Warfare 2 campaign difficulty levels explained
There are multiple difficulty levels in Modern Warfare 2 that let you decide how easy or how challenging the campaign will be.
Of course, this will have slight variations defending on what kind of playstyle you want to play. This is a very balanced and do-it-all build for the TAQ-56. It makes the recoil on the gun negligible and allows stability that makes aim improve by itself.
Contributor | Twitter @itsjustantnow
Doubling as a football (soccer) journalist following EFL League Two club Tranmere Rovers, Ant has a long-standing passion for gaming. That, combined with the drive to create content in the esports space led to the creation of The Rotation, a Call of Duty news platform.

domain: game


Anadolu Efes, our representative who will appear in the Final Four stage of the Euroleague next week, previously matched with Pınar Karşıyaka in the quarter finals of the ING Basketball Super League.
Ergin Ataman and his students, who managed to win with a comfortable game in the first match of the series, will be trying to win once again today and get their name written in the semi-finals.
Trying to be effective with Amath M’Baye in the first match of the series, the İzmir team could not stay in the match due to the lack of the expected contribution from the other scorers and were defeated by 24 points.
Pınar Karşıyaka, who will go to the park with the goal of victory in their own fields, will aim to carry the series to the last game by winning.
– Anadolu Efes won 3 in the matches of the two teams, who played a total of 4 times this season, including the regular season and cup competition, while Pınar Karşıyaka got their only win in the match played in their own hall.
– The two teams, who have faced each other 4 times this season, exceeded the 150-point threshold in all their matches.
MİSLİ.COM WHAT ARE THE MEMBERS PLAYING?
Misli.com members prefer Anadolu Efes to win by 26% in the second play-off match where Pınar Karşıyaka and Anadolu Efes will face each other.


text:
Political Instability and Inflation Volatility, Issues 2006-2212
International Monetary Fund, Sep 1, 2006 - Inflation (Finance) - 9 pages
The purpose of this paper is to empirically determine the causes of worldwide diversity of inflation volatility. We show that higher degrees of political instability, ideological polarization, and political fragmentation are associated with higher inflation volatility.
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Political Instability and Inflation Volatility (EPub)
Mr. Francisco Josť Veiga,Mr. Ari Aisen
Limited preview - 2006
3-year period According to Cukierman Agriculture percent Aisen and Veiga associated with higher becomes more volatile Change in oil CNTS coefficient is expected covering around 100 deep determinants determinants of inflation deviation of inflation economic variables Edwards estimated Executive changes explanatory variable fiscal policies freedom and higher GDP per capita greater ideological polarization greater political instability greater reliance groups fixed effects high inflation levels higher inflation volatility includes developing countries Index of economic inflation becomes lead to higher leads to greater levels and volatilities Log(Inflation Log[SD(Inflation logarithm of inflation lower economic freedom model of column Number oil prices panel data model percent of GDP polarization and fragmentation political and institutional political instability leads positive coefficient Real overvaluation reliance on seigniorage results Sahay SD(GDP growth seigniorage revenues standard deviation statistically significant Tabellini 1992 tests that consist Three-Year Period Trade percent Vegh volatile at higher volatile inflation rates volatility on growth

domain: finance


text:
SEATTLE — After three years, Starbucks Corp. is closing its three Princi Italian bakery bars. The New York bakery bar never reopened after government restrictions associated with the coronavirus pandemic forced a shutdown in March 2020. The other two locations in Chicago and Seattle are scheduled to close Sept 3.
A spokesperson confirmed that Starbucks will continue to serve Princi’s bread, pastries, pizzas and sandwiches at its Roasteries in Chicago, New York, Seattle, Shanghai, Milan and Tokyo.
Starbucks in July 2016 became an investor and global licensee of Princi, a high-end Italian bakery-cafe founded by Rocco Princi in 1986. In the fall of 2017, the company opened the first Princi location in the United States inside the Starbucks Reserve Roastery in Seattle, where artisanal baked foods are served alongside Starbucks Reserve coffees. Starbucks made Princi the exclusive food purveyor in its Roasteries and Starbucks Reserve store locations, a relationship that is expected to continue.

domain: food


text:
One feels like a charlatan writing of Garry Nichols’ extraordinary paintings. How to follow him? He suits up and dives to the depths. He sails the oceans like an albatross above them. The work is viewed from an original all-encompassing Artist’s eye. Framed from out of his delving mind; these fugitive mappings of experience by this lone Tasmanian, dowsing, desolate, angel.
Shored up in his Greenpoint, Brooklyn studio thirty-some years now. The large grand- scale paintings usually have a circular motif, a world spinning on its gyrating axis to contain the characters, plant and land forms that inhabit Nichols’ tenderly paint-daubed exploration of haunting, spellbinding vision. Threading out of the past, replete with the present, pointing to future.
Everything adds up but it doesn’t.
When you view a Nichols’ painting, you enter the realm of aboriginal genocide. You have the cruel, callous, civilized culture of deportation and the geography of Van Diemen's Land itself. It’s on that land you can still hear the first people calling out, the ancient aboriginal culture that settled there around 80,000 years ago, before being cut off from the rest of the continent by massive plate shifts. Renamed Tasmania in 1856. One hundred years later, the place of Nichols’ birth.
His ancestor arrived as a prisoner in this far-flung island in 1826; a 14-year-old London girl jailed for stealing clothes and under a sentence of death, has her sentence changed to deportation by the judge.
He is always calling this history up. How could he not, a born painter, a long time sophisticated habitué of the New York art scene? But he’s the possessor of a soul that pumps the blood, the memory, the imagination that haunts the paintings, is always back tracking, checking in, down under. The characters in the painting, men or women, aren't recognizably American or European. The landscape is a place only the artist has inhabited. The images are universal.
Everything adds up but it doesn’t.
Surreal has now become an overused and faded word. Perhaps the word is liminal. His paintings are cubist narrative day/night, laced with industrial plumbing, shipped with fools. Shimmering in backgrounds of gold-patterned leaves, a film that could have been shot by Rousseau.
There are clues, ships arriving, ships leaving. They are mostly steamers, more like Titanic's time than now. Faces ghosted off convict-carved bridges, heads festooned with surging cocks and lush delicately bearded cunts.
Where do you enter these profound studies of our humanity, our Divine planet?
Don't be fooled by the normalcy of his gentle, still-Aussie-tinted accent and friendly greeting. And be prepared to be led astray when you enter Nichols’ Greenpoint, Brooklyn studio. The paintings are epics, and like all true epics they suffer maelstroms.
His works have certain commonalities: a pattern, a weft, a whiplashed weave; they blink on and off, a palimpsest of constant change. Like sitting in an old-time launderette, the paintings cycling in glass-fronted washers; you peer at them, spinning your mind. The images although seemingly set in the landscape of childhood - youth breach, then reach maturity in one blink before our eyes. They glow, they fade, turn and tussle. Odysseus-like on their journey toward realization.
Yet there is no solution graphically, more a constant quest, the joy of an unfinished adventure. Like Alice in “ I wonder where the fuck I am Land,” he rabbits you down the hole of his vision. There is a method to this seeming madness.
He crafts his paintings as though viewed through a periscope, Framing the work in such a way, he presses you up against his soul’s porthole. You see the ship, the painting. Then the wave drops and you don’t.
And then you do.
It's a cunning painting strategy. He's honed it throughout his career. Every painting is now both a chapter heading and the completed chapter telling its tale. The colors amazing, realistic and not, all at once. These paintings, these vessels, travel the ocean of his mind. They sail not with certainty, not without possibility of shipwreck, not with a show of prowess for mock heroic sake.
They sail out under cover of a thousand secret nights, with a patina of darkness joined with the dawning light. Such subtly of tone; a shape, a shiver, a fall, a dive. He’s a painter on his watch, a dowser listening to creativity’s call. Spinning in his cups, cups of past, of present, of future, too, melded, abandoning all aesthetic achievement of lessons learned. Diving, falling, twisting in the subconscious, looking for the new in the old accretions, the revelatory.
Mr. Nichols. Your paintings outstrip this writing. They are based in their soul, their inquiry. Before writing you mark a history drawn out of your blood, seeped in the place of your birth. Long though you’ve trod the lanes of dear old Manhattan, written in the DNA of your painting hand are your ancient, native memories combined with that which you inhabit.
In the studio here in Greenpoint, when the door closes and the brushes come out, you tune back into the epic only you can account. This is the space, this liminal drift, this magical domain of mystery and memory. A kind of kaleidoscopic sucking-in and drawing down to the psychic depths. When a visitor enters, they are cast into the midst
of an Abundance, an Exasperation, the Untouchable, a whirling glimpse of the Unmentionable.
And although we're in Brooklyn, once land of the Dodgers, early home of Henry Miller, Walt Whitman editing the Brooklyn Eagle and a mass of modern artist’s studios, board one of Garry’s paintings, slip your culture’s shackles, open that Pandora’s box. The ticket’s destination changes as you travel.
Number 99 Commercial Street, Greenpoint Studio, Brooklyn, New York. Take your life belt with you. Your condoms and your pills for the morning after.
There's always a ship, waiting to sail out of there. On the Flying Dutchman they’re looking for mariners too. Hold on me hearties, there’s a Garry Nichols’ trip a-coming to you.

domain: artistic


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|Debra Spina, R.N., B.S.N., M.E.P. -C
Debra Spina R.N., B.S.N., M.E.P. –C has over 25 years of nursing experience including emergency medicine, critical care ICU and interventional radiology. Debra has been with Milwaukee Vein and Laser Center for over five years and has been practicing in the medical esthetic and phlebology field for the past six years. She is certified in dermal fillers, botulism toxin A (Botox), dysport, laser hair removal, IPL rejuvenation, microdermabrasion, chemical peels, non ablative laser resurfacing and sclerotherapy. She received her training and certification as a medical esthetic practioner from the Esthetic Skin Institute in Fort Lauderdale, FL. Debra is a member of the American College of Phlebology, American Academy of Aesthetic Medicine and the American Medical Esthetic Nurses Association in which she holds a position as a co-chair for the state of Wisconsin.
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