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George Anthony, grandfather of murdered toddler Caylee Anthony, was taken into custody by Daytona Beach police and brought to the Halifax Medical Center for evaluation. Anthony, 57, was found in a motel room after his family had reported him missing on Thursday. He had reportedly been sending text messages threatening to harm himself.
George Anthony, Cindy Anthony, Casey Anthony and the Mysterious Murder of Caylee Anthony
The heart-wrenching disappearance and murder of Caylee Anthony, a beautiful and innocent child, has attracted a tremendous amount of worldwide media attention. The case has been covered regularly and thoroughly by Greta Van Susteren, Nancy Grace, Geraldo Rivera and others. From the very beginning of the case, suspicion was cast on Casey Anthony, Caylee’s 22-year-old mother. Perhaps the fact that she did not report her daughter missing for an entire month after her disappearance had something to do with it.
Tragically, any hope that Caylee Anthony would be found alive was shattered when, on December 19, 2008, the Orange Country Medical Examiner confirmed that the human remains found close to the home of the Anthony family were those of Caylee.
Cindy and George Anthony Defend Casey Anthony
From the moment Casey Anthony became a suspect in the murder of Caylee Anthony, her parents, Cindy and George Anthony, have defended her innocence. Time and again, George Anthony has proclaimed his daughter’s innocence, emphatically insisting that she had nothing to do with the disappearance and murder of Caylee. This despite the fact that a number of mental health professionals have gone on record saying that Casey Anthony exhibits the classic signs of a sociopath.
“The disorder is recognizable by a lack of concern for others, as evidenced by her behavior after Caylee disappeared. She did not call 911 to report her daughter missing; that task was left to her mother, weeks after Caylee disappeared. While her daughter was missing, Casey was out “clubbing”, buying lingerie and other sundry items for herself from Target, and cooking dinner for her boyfriend and his roommates,” writes Dr. Julian Mitchell on Blogcritics.
Dr. Mitchell notes that sociopaths consider friends, acquaintances and family members to be expendable. A sociopath moves on once others have been “bled dry.” One has to question whether George Anthony’s desperate behavior reflects an awareness of his daughter’s true colors. An awareness that he can no longer deny.
Caylee Anthony’s grandfather reported missing, later found, CNN.com
Casey Anthony: Profile Of A Sociopath, Dr. Julian Mitchell, PhD, blogcritics.org, October 19, 2008


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There is a new world in your hands
There is a new world in your hands and the way in which we care has grown bigger!
The world has found itself in a challenging new situation for the past couple of years. And bringing a new life into this new era it's demanding for any parent, new or experienced or not, especially when we need to be so distant from our loved ones.
Natura's Mamãe e Bebê line has helped reinforce the bond between moms, dads, and their newborn babies. With products that are 100% vegan, with being 96% of natural ingredients for daily use products and 87% for perfumery products, these are perfect and safe to use from day one, given due to mother's sensitivity to strong smells and the baby’s sensitive skin, it's just perfect and safe to use from day one.
We know that the distancing has caused not only routine difficulties at work and in our daily activities, but it also has allowed us to dig deeper into new approaches to raising a child in this modern era, which demands much more of all parents than what they thought it would.
This is why we are always concerned about providing different products for different stages of the parent's needs, such as:
- The best thing that parents can provide for their new baby is essentially caring for their well-being from day one. With Natura's Mamãe e Bebê line, we allow moms and dads to safely nurture and care for the baby safely with 100% vegan products that are perfect for your baby’s sensitive skin and are approved by, pediatricians approved, and just perfect products for your baby sensitive skin.
- Relaxing Mamãe e Bebê is recommended for the baby's first months of life since resting has vital importance due to the baby's organism’s need to metabolize nutrients, storing all that they have learned, and to produce the hormones that the body needs. So, a safe and comfortable environment, with a fixed routine and the relaxing touch of mom and dad, are essentials for a good night’s sleep.
- Shantala is Natura's Mamãe e Bebê Massage Oil, recommended for a moment of pure care and bonding between baby, mom and dad. Its formula is 100% vegan and safe, containing only the essentials for massaging the baby's sensitive skin. It is smooth to the touch and hydrates the skin.
- Mothers are fundamental figures during the baby's development. So it's very important for them to take care of themselves, and to let their love it flourish love during the pregnancy. Natura's Mom Care is a range of products that provides consisting of a daily routine of self-massage, well-being, self-esteem, and relaxation routine, helping to prevent stretch marks. The line is specially developed for the mom's sensitive sense of smell.
Natura understands that there will be even greater challenges and also rewards after all these troubling times, but we also want to be closer with every mom and dad in the best way possible we know how: providing safer, more natural, and healthier ways of strengthening family bonds.

domain: health


Urban Hippie / CSA
Aug 30, 2012
Produce is often picked 4 to 7 days before being placed on supermarket shelves. The longer it takes to get produce from farm to table, the more likely it will be to lose valuable nutrients. When produce is harvested ripe, it is often easier to chew and digest and it tastes so much better. Ripe produce is sweeter and more flavorful. Produce is shipped an average of 1,500 miles before being sold (and that is only in the U.S.). Buying local saves energy and helps the environment. Shipping food cross-country in trucks both burns fuel and emits pollution. You buy produce...


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There has been a lot of noise lately in Washington, D.C. about the potential raise to the national minimum wage from its current rate of $7.25 per hour to either $9 or $10.10 per hour. There is a mindset among some Americans that the minimum wage, or entry-level wages, should be substantial enough for an adult head of a household to be able to support a family. That is completely unrealistic.
It seems that some Americans have lost the perspective of who is the target for minimum wage jobs, which are not meant to be career jobs. The people earning the wage are typically entry-level workers — namely, younger individuals with little education and or minimal skill sets.
Furthermore, there seems to be a lack of understanding (of who) would ultimately pay for these suggested minimum wage increases. Most likely, it will be the minimum wage workers themselves.
It’s easy to understand why the federal policy makers would find an increase in the minimum wage so attractive. Unlike boosting welfare programs, raising the minimum wage doesn’t increase government spending. The government is passing their societal obligation of taking care of people who elected them to the business owner, while ignoring the simple business principal of Law of Demand, which says that when the price of labor rises, the quantity demanded will fall (assuming other things are constant). That same law tells us that quantity demanded (i.e., the number of jobs for low-skilled workers) will decrease more in the long run than in the short run, as employers switch to labor-saving methods of production (i.e. automation, outsourcing overseas, improved technology), and unemployment will increase.
If the minimum wage exceeds the prevailing market wage (determined by supply and demand), some workers face the strong possibility of losing their jobs or having their hours reduced. There is abundant evidence that a 10 percent increase in the minimum wage leads to a 1 to 3 percent decrease in employment of low-skilled workers in the short run, and to a larger decrease in the long run, along with rising unemployment.
As Forbes contributor James Dorn astutely points out:
“…advocates of higher minimum wages confuse cause and effect. They think a higher minimum wage causes incomes to go up for low-skilled workers and doesn’t destroy jobs. Workers are assumed to have higher wages and retain their jobs as a result of government policy — even though they have done nothing to improve their job skills. But if a worker is producing $5.15 per hour and now the employer must pay $9 per hour, there will be little incentive to retain (him or her). There will also be little incentive to hire new workers. Without an increase in the demand for labor — that is, an increase in labor productivity due to better technology, more capital per worker, or additional education — a higher minimum wage will simply price some workers (the least productive) out of the market, and their incomes will be zero.”
An increase in the minimum wage is not a cure for poverty. Indeed, in 2005, research published in the Journal of Human Resources showed “the net effect of higher minimum wages is … to increase the proportion of families that are poor and near-poor.” Thus, the minimum wage tends to increase, not decrease, the poverty rate.
Politicians promise low-skilled employees a higher wage, but that promise cannot be kept if employers cannot profit from retaining those employees or hiring similar employees. Jobs will be lost, not created; and unemployment will rise as more workers search for jobs but can’t find any at the above-market wage. If the minimum wage increase cuts into profits too deeply, the hiring of low-wage workers will be more expensive compared to other investments, such as new machinery or improved technology. Businesses could further reduce their headcount of low-wage workers and shift their spending toward other non-human revenue producing programs, such as automated systems.
Instead of forcing a minimum wage increase, the federal government should consider methods and programs that allow the low-skilled workers to improve their skill sets, making them more desirable in the workforce. To paraphrase an old adage: give a man a minimum wage increase and you feed him for a day; teach a man improved job skill sets and you feed him for a lifetime.
Todd Palmer is founder and president of Troy-based Diversified Industrial Staffing and Diversified PEOple LLC.

domain: sociology


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I am breathing a sigh of relief. The hectic time of directing "Taking Steps" is at last over. Eight weeks of intensive rehearsals led to the opening of the play in Hastings, and eight performances at the Stables. These were well received, and we had an average attendance of some 70%. After a mere week's break, we were on a flight to the USA, and performances in Chicago Heights at The Drama Group. I had been working on the set with the crew in the USA by email. I liken it to building a set by remote control! But it worked. IN The Stables, we had a stage about 20ft wide. In Chicago Heights, the width was almost 50ft, so we had to do some adjusting to stage positions. Also, the audience was so close to the actors that we were afraid the fight scene would result in one of our actors plunging into the laps of the front row. We did manage to avoid that, but with the stage on the same level as the two front rows, people coming in often walked acros the stage itself. Very disconcerting.
Our hosts were the epitome of hospitality, taking us to and fro in the delightful suburb of Chicago Heights. This involved taking each of us from our temporary home to the theatre, and after each show to a wonderful tavern at Flossmoor, as well as to a station to allow us to travel into Chicago itself. They could not have been more accommodating, and generous. Many a boozy hour was spent at the Flossmoor Tavern testing the 16 beers that were brewed on the premises. My favourite was BAD (barrel aged Dubbel). I am not sure what the specific gravity was, but two glasses were enough! The shows went down a storm, and we even got a standing ovation one night.
Chcago is a wonderful city, set on the shores of Lake Michigan, with spectacular skyscrapers all around the central area.

domain: geography


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Hunter Valley accommodation - book from the largest selection and find a great deal.
NEW BLOG POST!
A new blog post is now available to read by one of our ambassador bloggers, Siobhan Curran! This time Siobhan follows the art trail through the Hunter Valley & stays in the best accommodation she has ever stayed in...find out where it is!!

domain: literature


text: Various – Dubstep Bass & Movement! Volume 2 · Tracklist · Notes · Barcode and Other Identifiers · Reviews · Let's manage your privacy · We and our. Acheter Various - Dubstep Bass & Movement! Volume 2 - PARA Music Publishing, World Club Music - CD, Comp - ПРЗ CD, inclut Starscream, Animal. Album Dubstep Bass & Movement! Vol. 2, information about artists and all songs. 歌手, Various Artists. 唱片公司, World Club Music / TME. [mp3] Listen to online Various - Dubstep Bass & Movement! Volume 3, or download mp3 tracks: download here mp3 release album free and without registration. Top Drawer Digital Present Back And Forth Volume 2 [Dubstep J-Tek Future Jungle] With Various Artists; Release Date Seal Bass Dubstep Mix. Various Artists; Release Date ; Label Bass Tank; Catalog CUBD $ 2. Continuous Dubstep Trap Mix Original Mix · Various Artists. The resulting sound is a timbre that is punctuated by rhythmic variations in volume, filter cutoff, or distortion. This style of bass is a driving factor in. While artists like Lady Sovereign and Dizzee Rascal have expounded definitely-British sounds to the United States' audience, the movement hasn't really caught. 40 SONGS • 2 HOURS AND 59 MINUTES • MAY 31 Want to discover art related Thong Song (Dubstep Remix) E. Bass Music Wiki is a FANDOM Music Community. Dubstep Junkies - Shotgun (Featuring The Anix) The World of Lateinamerikanische Tänze, Volume 2 The Dilemma - Music from the Motion Picture.

domain: artistic


This round of funding will enable us to further productize our platform and unify new data streams from a multitude of business-critical applications. As explained by Tuan Palmer, a Founding Partner at Angels 3.0, “This will enable Tickr to do for enterprise data what Netflix is doing for video programming, by providing a single unified interface for a very wide range of sources, enhanced by filtering, post-processing, API support, and smart search.”
We will also use the funds to build out and accelerate Tickr’s partnership program. Many different types of businesses can benefit from reselling our platform to their existing customer base. Tickr offers a white-labeled integration that can boost revenue by expanding deal size, with zero development overhead on the reseller’s part. Reselling Tickr creates upsell opportunities as new data integrations are added. It also allows resellers to attract new customers and increase their market share by integrating new information and insight in to their existing product platforms
We’re excited about the potential we are unlocking here, and look forward to working with you to realize our vision over coming months.


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Los Angeles County Public Health officials on Friday confirmed 226 new deaths and 4,761 new cases of COVID-19 countywide with 24,541 total cases in the Santa Clarita Valley. In addition, Public Health noted that despite a decline in hospitalizations, deaths may remain high for two more weeks.
The Santa Clarita Valley has now seen a total of 24,541 total cases — 131 more cases since Thursday — and 229 deaths since L.A. county’s first confirmed COVID-19 infection on January 26, 2020.
Public Health officials are closely monitoring the number of deaths. More than 1,000 new COVID-19 deaths have been reported since Monday.
“We wish healing and peace to the families mourning their loved one lost to COVID-19,” said Barbara Ferrer, PhD, MPH, MEd, Director of Public Health.
With Super Bowl Sunday closing, Public Health officials remind residents to avoid gathering with people outside of their household.
Gathering with people outside of your household, especially in settings where people are shouting, chanting, or singing and not distancing can easily lead to increased cases of COVID-19, serious illness, and more deaths.
Public Health recommends residents to play it safe and enjoy the Super Bowl with people you currently live with, and to connect virtually with other friends and family who live outside of your household. Please do not gather with people from outside your household to watch the Super Bowl.
“I want to remind everyone how important it is to celebrate the Super Bowl only with members of your household,” Ferrer said. “This fall and winter, we learned painful and tragic lessons about how gatherings and celebrations lead to transmission of COVID-19 that ultimately results in a great deal of serious illness and death.”
To date, Public Health identified 1,138,764 positive cases of COVID-19 across all areas of L.A. County and a total of 17,764 deaths.
Testing results are available for more than 5,573,000 individuals with 19% of people testing positive. Today’s daily test positivity rate is 8.6%.
There are 4,796 people with COVID-19 currently hospitalized and 28% of these people are in the ICU.
To date, Public Health officials have identified 1,129,503 positive cases of COVID-19 and a total of 17,308 deaths countywide.
See more SCV and L.A. County info later in this report.
Hospital Surge Order Rescinded
The California Department of Public Health announced today that due to improving conditions at hospitals statewide, including the continuing decline in positive COVID-19 cases and hospitalizations, it has rescinded the Hospital Surge Public Health Order.
The Hospital Surge Public Health Order was put into place on January 5, 2021, as an emergency measure to address an urgent statewide crisis in ICU capacity that was caused by unprecedented exponential community transmission combined with scarce hospital resources in equipment, space, and staffing.
The Order served to spread the COVID burden across hospitals and ensure that all patients were receiving a high quality of care to prevent loss of life.
Current data reflecting declining cases, hospitalizations, test positivity and transmission rates indicate that the acute phase of the unprecedented surge experienced at the end of 2020 has abated sufficiently for hospitals to resume elective surgeries and other conventional standards of care.
As community transmission has decreased, so has the burden on the hospital system, including lower hospital admissions, lower levels of patients boarded in the emergency department and shorter ambulance patient offload times.
Consequently, while the status of COVID-19 in California remains serious, the emergent Hospital Surge Order can now be rescinded.
California Friday Snapshot
Statewide, as of Thursday, February 4, California Department of Public Health officials confirmed a total of 3,308,468 COVID-19 cases (up 14,021) with 43,024 deaths from the disease (up 558) since the pandemic began.
There are 12,863 confirmed hospitalizations and 3,432 ICU hospitalizations in the state, continuing a downward trend.
As of Thursday, Feb. 4, local health departments have reported 88,481 confirmed positive cases in health care workers and 358 deaths statewide.
There have been 43,433,104 tests conducted in California. This represents an increase of 197,777 during the prior 24-hour reporting period.
The 7-day positivity rate is 5.8% and the 14-day positivity rate is 6.6%, continuing a downward trend.
Numbers do not represent true day-over-day change as these results may include cases from prior to yesterday.
As of Friday, providers have reported administering a total of 4,199,626 vaccine doses statewide. Those numbers also do not represent true day-to-day change as reporting may be delayed.
The CDC reports that 6,803,450 doses have been delivered to entities within the state, and 6,981,275 vaccine doses, which includes the first and second dose, have been shipped.
See more California information later in this report.
Screencap from the Johns Hopkins University’s Center for Systems Science and Engineering COVID-19 dashboard, showing COVID cases in the United States as of Friday afternoon, February 05, 2021.
U.S. Deaths Exceed 450,000 People; Global Deaths Nearing 2.3 Million People
Worldwide, 105,328,463 people have been infected by COVID-19 while 2,296,291 people have died of the virus as of 3:22 p.m. Friday Pacific Time, according to data compiled by Johns Hopkins University.
In the U.S., more than 26,792,699 Americans have been diagnosed with COVID-19. The number of people in the U.S. who have died due to the virus has now surpassed 458,985.
With 4.25% of the world’s population (328.2 million) and more than 20% of the confirmed COVID-19 cases, the U.S. also continues to lead the world in deaths.
By comparison, Brazil (population 209.5 million) is No. 2 in deaths with 230,034, and No. 3 in cases with 9,447,165. India (population 1.353 billion) is No. 2 in cases, with 10,802,591 confirmed infections and No. 4 in deaths with 154,823 as of Friday afternoon.
Henry Mayo Newhall Hospital Friday Update
Note: There was no available data released Friday. Henry Mayo Newhall Hospital releases complete statistics weekly, usually on Wednesdays, unless one or more new deaths occur.
The two new deaths reported Wednesday brought Henry Mayo Newhall Hospital’s COVID fatalities to 123 since the pandemic began, spokesman Patrick Moody said Wednesday.
In November, eight COVID-19 patients died at Henry Mayo. In December, four times that many people — 34 — died at the hospital.
Henry Mayo reported 49 patient deaths due to COVID-19 in January 2021, and two fatalities so far in February, Moody confirmed.
As of Wednesday, no COVID-19 cases are pending, 63 patients were hospitalized in dedicated COVID-19 units receiving ICU-level care (down seven from Monday), and a total of 1,034 COVID-19 patients have been treated and discharged, Moody said.
Privacy laws prohibit Henry Mayo from releasing the community of residence for patients who die at the hospital; residence info is reported by the L.A. County Public Health COVID-19 dashboard, which generally lags 48 hours behind.
Santa Clarita Valley Friday Update
As of 6 p.m. Wednesday, the latest update of the L.A. County Public Health dashboard recorded 229 deaths among Santa Clarita Valley residents since the pandemic began.
Of the 229 SCV residents who have died, 197 lived in Santa Clarita, 12 in Castaic, six in Acton, four in Stevenson Ranch, three in unincorporated Canyon Country, three in Agua Dulce, one in Newhall, one in unincorporated Bouquet Canyon, one in Lake Hughes, and one in Val Verde.
Of the 24,541 confirmed COVID-19 cases reported to Public Health for the SCV to date, the community breakdown is as follows:
* City of Santa Clarita: 17,906
* Castaic: 3,455 (incl. Pitchess Detention Center & North County Correctional Facility*)
* Stevenson Ranch: 971
* Canyon Country (unincorporated portion): 724
* Acton: 405
* Val Verde: 286
* Agua Dulce: 233
* Valencia (unincorporated portion west of I-5): 167
* Saugus (unincorporated portion): 122
* Elizabeth Lake: 70
* Newhall (Unincorporated portion): 66
* Bouquet Canyon: 41
* Lake Hughes: 37
* Saugus/Canyon Country: 29
* San Francisquito/Bouquet Canyon: 14
* Sand Canyon: 15
*Note: The county is unable to break out separate numbers for Castaic and PDC/NCCF because the county uses geotagging software that cannot be changed at this time, according to officials. Click here for the LASD COVID-19 dashboard.
L.A. County Vaccine & Vaccination Update
Next week there will be 360 vaccination sites operating in L.A. County. While COVID-19 vaccine supply remains very limited, Public Health has built an extensive network with pharmacies, federally qualified health centers, hospitals, health clinics, and community vaccination sites, including six large-capacity sites.
* Dodger Stadium (operated by the city of Los Angeles)
* California State University, Northridge, 18111 Nordhoff St, Northridge 91330
* Pomona Fairplex, 1101 W McKinley Ave, Pomona 91768
* The Forum, 3900 W Manchester Blvd, Inglewood 90305
* L.A. County Office of Education, 12830 Columbia Way, Downey 90242
State and federal authorities will open another large-capacity vaccination site at California State University, Los Angeles, targeting underserved communities, that’s due to open February 16, Gov. Newsom said Wednesday.
More than 1,051,000 COVID-19 vaccine have been administered across the county. Of those vaccinated, 204,449 people have received second doses. Every person vaccinated is guaranteed a second dose.
Due to the limited supply of vaccine, appointments fill up fast and the majority of appointments at the large capacity vaccination sites are booked for second doses.
We are hopeful in the coming weeks that more vaccines will become available.
Yesterday, Johnson and Johnson submitted an application for Emergency Use Authorization to the FDA and we look forward to reviewing the data.
Public Health asks that residents don’t sign up to be vaccinated if it is not their turn. Signing up for a vaccine before it is your turn may take away an appointment from a high-risk person who is unlikely to be vaccinated when you show up for the appointment.
“Please do the right thing: wait your turn for a vaccine and allow those eligible for the vaccine to register and be vaccinated,” Ferrer said.
As the county moves through its vaccination program, Public Health officials are constantly aligning strategies to reach the most vulnerable. As a reminder, the agency is prioritizing vaccinating individuals 65 years and older, while working with its partners and the state to further plan for vaccinating additional workers in the prioritized groups.
Given the limited weekly shipments, a limited vaccine supply requires balancing priorities. While the state is updating its distribution plans, the county must also look at how best to protect the most vulnerable with the goal of reducing mortality. Officials ask for patience until the county receives all the vaccine it needs to get to everyone who wants to get vaccinated.
“While we continue efforts to ensure that vaccine makes it into more arms, we implore everyone to stay home as much as possible, to not gather, and to do everything you can to prevent transmission of the virus,” Ferrer said.
Public Health’s www.VaccinateLACounty.com connects residents eligible for COVID-19 vaccinations to appointment registration links and much more.
As appointments become available, residents with internet access and a computer are urged to use the website to sign up. For those without access to a computer or the internet or with disabilities, a call center is open to help schedule appointments at 833-540-0473, daily from 8 a.m. to 8:30 p.m.
L.A. County Demographics — Deaths by Age Group
Of the 226 new deaths reported today, 70 people who passed away were over the age of 80, 83 people who died were between the ages of 65 and 79, 37 people who died were between the ages of 50 and 64, 18 people who died were between the ages of 30 and 49, one person who died was between the ages of 18 and 29, and one death is under investigation. Eleven deaths were reported by the City of Long Beach and five death were reported by the City of Pasadena.
L.A. County Demographics — Cases by Age Group (excluding Long Beach and Pasadena)
* 0 to 4: 21261
* 5 to 11: 51569
* 12 to 17: 64497
* 18 to 29: 256296
* 30 to 49: 359372
* 50 to 64: 207515
* 65 to 79: 82397
* over 80: 29761
* Under Investigation 6675
L.A. County Demographics — Hospitalizations by Age Group
Residents over 80 years old have consistently experienced the highest rates of hospitalization among all age groups in L.A. County followed by residents 65 to 79 years old, and residents 50 to 64 years old.
Recent data indicates obesity, cardiovascular disease and diabetes are the most common underlying health conditions among people hospitalized with COVID-19.
Many people have multiple underlying health conditions.
L.A. County Public Health’s Reopening Protocols, COVID-19 Surveillance Interactive Dashboard, Roadmap to Recovery, Recovery Dashboard, and additional things you can do to protect yourself, your family and your community are on the Public Health website, www.publichealth.lacounty.gov.
California Blueprint for a Safer Economy
Governor Newsom’s Blueprint for a Safer Economy imposes risk-based criteria on tightening and loosening COVID-19 allowable activities and expands the length of time between changes to assess how any movement affects the trajectory of the disease.
With the Regional Stay at Home Order rescinded statewide as of January 25, all counties are now under the rules and framework of the Blueprint for a Safer Economy and color-coded tiers that indicate which activities and businesses are open based on local case rates and test positivity.
* 54 counties are currently in the Purple Tier (including Los Angeles County)
* 1 county is currently in the Red Tier (Mariposa)
* 3 counties are currently in the Orange Tier (Alpine, Sierra, Trinity)
Vaccinate All 58
In order to increase the pace of COVID-19 vaccine distribution to those at greatest risk, the state is prioritizing individuals 65 and older to receive the vaccine as demand subsides among health care workers. This effort will help to reduce hospitalizations and save lives.
To sign up for a notification when you’re eligible for a vaccine, visit myturn.ca.gov.
‘Safe Schools for All’ Plan
Governor Newsom launched the Safe Schools for All Hub as a one-stop-shop for information about safe in-person instruction.
For more information on the transparency, accountability, and assistance measures related to California’s Safe Schools for All plan, visit the hub.
California Public Health has issued an updated travel advisory. Postponing travel and staying home is the best way to protect yourself and others from COVID-19.
Non-essential travelers from other states or countries are strongly discouraged from entering California and should adhere to the state’s self-quarantine procedures for 10 days.
California Demographics: Health Equity Dashboard
The COVID-19 pandemic has highlighted existing inequities in health that are the result of structural racism and poverty, and the disproportionate prevalence of underlying conditions such as asthma and heart disease among Latinos and African Americans.
As part of its commitment to reduce health inequities and ensure the best outcomes for all Californians, the state has launched a Health Equity Dashboard on www.covid19.ca.gov/equity/ that tracks California’s health equity measure and data by race and ethnicity, age group, and sexual orientation/gender identity.
The testing turnaround dashboard reports how long California patients are waiting for COVID-19 test results. California has worked to reduce testing turnaround times in recent weeks to help curb the spread of the virus.
During the week of January 17 to January 23, the average time patients waited for test results was 1.2 days. During this same time period, 74% of patients received test results in one day and 92% received them within two days. The testing turnaround time dashboard (PDF) is updated weekly.
All four tiers in the Testing Prioritization Guidance originally dated July 14, 2020, have equal priority for testing.
Multisystem Inflammatory Syndrome in Children (MIS-C)
Each week, the California Department of Public Health updates the number of cases of Multisystem Inflammatory Syndrome in Children (MIS-C) reported in the state.
As of February 1, there have been 200 cases of MIS-C reported statewide, 12 more than the previous week. To protect patient confidentiality in counties with fewer than 11 cases, we are not providing total counts at this time.
MIS-C is a rare inflammatory condition associated with COVID-19 that can damage multiple organ systems. MIS-C can require hospitalization and be life-threatening. Parents should be aware of the signs and symptoms of MIS-C including fever that does not go away, abdominal pain, vomiting, diarrhea, neck pain, rash, bloodshot eyes, or feeling tired.
Contact your child’s doctor immediately if your child has these symptoms. Early diagnosis and treatment of patients are critical to preventing long-term complications.
Protect Yourself and Your Family: Your Actions Save Lives
Protect yourself, family, friends, and community by following these prevention measures:
* Staying home except for essential needs/activities following local and state public health guidelines when patronizing approved businesses. To the extent that sectors are re-opened, Californians may leave their homes to work at, patronize, or otherwise engage with those businesses, establishments or activities.
* Adding your phone to the fight by signing up for COVID-19 exposure notifications from CA Notify.
* Answering the call if a contact tracer from the CA COVID Team or local health department tries to connect.
* Following guidance from public health officials.
What to Do if You Think You’re Sick
Call ahead: If you are experiencing symptoms of COVID-19 (fever, cough or shortness of breath), call your healthcare provider before seeking medical care so that appropriate precautions can be taken.
It’s important if someone thinks they could be positive for COVID-19 and are awaiting testing results to stay at home and act as if they are positive. This means self-isolating for 10 days and 72 hours after symptoms and fever subside.
If a person tests positive for COVID-19, they should plan on receiving a call from a public health specialist to discuss how to protect themselves and others, find out where they may have been, and who they were in close contact with while infectious.
California COVID-19 Data and Tools
A wide range of data and analysis guides California’s response to COVID-19. The state is making the data and its analytical tools available to researchers, scientists and the public at covid19.ca.gov.
The Sand Canyon Resort project is scheduled to return before Santa Clarita Planning Commissions Tuesday with a series of revisions, following multiple concerns raised by both commissioners and residents.
The Valley Industry Association will welcome College of the Canyons Chancellor Dr. Dianne G. Van Hook as the keynote speaker for the March VIA Virtual Series taking place Tuesday, March 16, from 11:00 a.m to 12:15 p.m.
The California Department of Motor Vehicles announced a new round of extensions for commercial driver’s licenses expiring through May 31 that will help commercial drivers focus on delivering essential products and supplies during the COVID-19 emergency.
The Los Angeles County Department of Consumer and Business Affairs (DCBA) announced Thursday the upcoming launch of the Los Angeles Online Dispute Resolution (LA-ODR) program, in collaboration with the Superior Court of California, Los Angeles County and its Dispute Resolution Program (DRP), and the Center for Conflict Resolution.
Santa Clarita City Council members declined Tuesday to administer $6.8 million in state rental assistance funds for eligible residents who have been impacted by the COVID-19 pandemic and let the state handle those dollars but approved creating a program for them with $6.3 million from the federal government.


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Statistical learning in SLI (Lammertink et al., 2017)
journal contributionposted on 2017-11-17, 16:19 authored by Imme Lammertink, Paul Boersma, Frank Wijnen, Judith Rispens
Purpose: The current meta-analysis provides a quantitative overview of published and unpublished studies on statistical learning in the auditory verbal domain in people with and without specific language impairment (SLI). The database used for the meta-analysis is accessible online and open to updates (Community-Augmented Meta-Analysis), which facilitates the accumulation and evaluation of previous and future studies on statistical learning in this domain.
Method: A systematic literature search identified 10 unique experiments examining auditory verbal statistical learning in 213 participants with SLI and 363 without SLI, aged between 6 and 19 years. Data from qualifying studies were extracted and converted to Hedges’ g effect sizes.
Results: The overall standardized mean difference between participants with SLI and participants without SLI was 0.54, which was significantly different from 0 (p < .001, 95% confidence interval [0.36, 0.71]).
Conclusion: Together, the results of our meta-analysis indicate a robust difference between people with SLI and people without SLI in their detection of statistical regularities in the auditory input. The detection of statistical regularities is, on average, not as effective in people with SLI compared with people without SLI. The results of this meta-analysis are congruent with a statistical learning deficit hypothesis in SLI.
Supplemental Material S1. Search syntax and Boolean operators for databases.
Lammertink, I., Boersma, P., Wijnen, F., & Rispens, J. (2017). Statistical learning in specific language impairment: A meta-analysis. Journal of Speech, Language, and Hearing Research, 60, 3474–3486. https://doi.org/10.1044/2017_JSLHR-L-16-0439

domain: psychology


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LAY IT DOWN Making art and writing can be a way of laying down a burden.
Get it out, and you no longer need to hold it.
Equip the reader or viewer to experience your art. An author’s note, introduction, or artist’s statement can help your work speak. The work starts living for itself.
If you’re in the practice of serving other artists—coach, editor—it can be tempting to “help” them. You want them to achieve their goals, and you have tools for that: templates, schedules. You can see where the piece could go, and you want to bring them there. You’re here to help.
We’ve been taught our role is one of service. “How can I help you?” can be generative, an invitation. “You are the authority on yourself and your work. Please tell me what you need.”
But even asking the question can suggest a power imbalance. The question can imply a problem, and the inherent suggestion is that “problem” is the artist. “How can I help you get this? How can I help you so you work with me better? How can we move your work into the realm of ‘real art’?”
What the other person might need more than help is acceptance. “I see you. I see you trying your best. You make good decisions. I hear what you’re telling me. I accept you just as you are, even if you’re not where you want to be yet. I see you carrying the burden. Lay it down. Or let me carry some of that burden for you. Let’s do this together.”
Acceptance of the maker can bring the artwork to new heights. Acceptance of the person can allow the relationship to truly begin.
Mary Oliver wrote, “Attention is the beginning of devotion.” Acceptance continues the path, the commitment to co-creation.


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On March 11th, 2020, Adam Silver suspended the NBA Season due to COVID-19. For sports fans, it has been rough. You never know what you got until its gone. Even with a the Wizards having a 24-40 record on the season, fans would settle for any type of basketball; regardless of the quality. Say less!
This is the most recent NBA2K20 Simulation. These games are broadcasted on the NBC Sports Washington Channel as if the regular season has not been suspended. The Wizards will broadcast Wizards 2K Simulations for the rest of the regular season.

domain: sports


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As follow-up to the December 2019 Pathways to Health, resident-led event, we hosted two resident grant workshops to improve community member access to existing city and county resources.
February 2020 Workshop
On February 1, 2020, we hosted a Neighborhood Services Workshop in which residents applied for the City of Fort Collins Mobile Home Park Neighborhood Improvement and Community-Building Mini Grants, a funding stream which was established as a direct result of the December Pathways event. Residents applied for funds for safety lighting, tree trimming, sewer scoping, and other community identified priorities. As a group, the resident coalition applied for streetlights to provide better, more comprehensive neighborhood lighting. This grant workshop minimized barriers by enabling residents to complete the application during the session thanks to facilitation from community leaders, interpreters, and city staff. Over 30 applications were completed on February 1. Moving forward, interest remained high for this type of event. This workshop resulted in the resident coalition receiving $5,000 for safety lighting and $5,000 for tree trimming from City of Fort Collins Neighborhood Services.
March 2020 Workshop
On March 7, 2020, we hosted another grant workshop during which residents not only applied for Neighborhood Services grants, but also applied for the City of Fort Collins Department of Recreation Reduced Fee Program, Colorado Low-Income Energy Assistance Program, Fort Collins Utilities Payment Assistance, and Alianza NORCO Citizenship and Driver License Workshops.
Through the second phase of Pathways to Health events, we have found that barriers to active living practices and the difficulties accessing safe routes to prioritized destinations are caused not only by direct challenges, but also by systemic power differentials that affect the quality of life and opportunities of Latinx individuals in Fort Collins mobile home parks. By focusing on empowerment and community needs at a more holistic macro level, we facilitated residents’ effort to become their own advocates for individual, neighborhood, and community level change, which results in better health.


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SAN RAFAEL -- Seven police departments were called to aid San Rafael police after violence erupted when officers broke up a large crowd at a sideshow early Saturday, authorities said.
The confrontation came after peaceful celebrations of Guatemalan Independence Day had ended and throngs gathered at several locations in the Canal neighborhood, police said Saturday.
About 1,500 people celebrated the independence events at Northgate Mall in Terra Linda and in a parking lot in the 1100 block of Andersen Drive. As those events ended, large gatherings took place in the Canal neighborhood, leading to traffic jams, police said.
Officers estimated that hundreds of vehicles and more than 1,000 onlookers were present.
At midnight, officers began to break up the crowd at a sideshow, an illegal display of automotive stunts, at Canal Street and Medway Road. When violence flared, police broadcast an emergency request for aid from other police agencies.
The Marin County Sheriff's Office, California Highway Patrol, Novato Police, Central Marin Police Authority, Fairfax Police, Ross Police, and Mill Valley Police responded, clearing the area by 1:45 a.m., San Rafael police said.
Besides the crowd issues Friday night and early Saturday, police responded to shootings that wounded two people and a stabbing that wounded one.
A 25-year-old man who was shot twice in the 100 block of Novato Street was taken to a hospital with life-threatening injuries. Separately, a 20-year-old man turned up at a hospital emergency room with a non-life-threatening gunshot wound but refused to cooperate with investigators.
A 24-year-old man was stabbed in a fight and was taken to a hospital with non-life-threatening injuries.
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domain: law


Currently there are 87 homes for sale and 5 vacant land parcels for sale in 30017
Learn More About Grayson, Georgia
Grayson is a very small city of just about 3,000 people situated southeast of the heart of Gwinnett County in the state of Georgia. It covers a total area of about two and a half square miles, with a little over 2 percent of this made up of water. The community is governed by a mayor and a city council comprising four members.
First settled in 1820, Grayson was established in 1879 by James Paterson McConnell, who bought 80 acres of land in the area. He constructed a building that housed the community’s first post office alongside several businesses. Grayson was previously known as Trip, a name given by a Lawrenceville public figure called J.D. Spence, who described coming to McConnell’s place as a trip. Postmaster John Ellery Jacobs requested in 1901 that the name of the town be changed to Berkley. But there was already a community with that name in the state. Jacobs then suggested Graymount (inspired by unobstructed view of the Stone Mountain), but it was also taken, making him to settle for Grayson.
Although Grayson has a rather low population, it has in fact experienced significant growth in recent years. The resident count was just 765 as of 2000 census. But it has today surpassed 3,000, driven by exurban growth in the eastern section of Gwinnett County. This community, which is a decent choice for the unhurried lifestyle, boasts of very high diversity in terms of the races of those calling it home. A significant proportion of the residents are born outside the United States.
A large number of the homes in Grayson were built in recent years to cater to the rise in the number of residents. The influx of people to this location has made local real estate to rank among the most valued in the county and state. Some of the popular communities among home buyers in this tiny city includes Wheatfields Reserve and Sawyer Farms.
The small-town ambiance of Grayson makes it a very popular place for families. There are many families here creating opportunities for parents and children to establish new social relationships. The community boasts of several family-oriented services. Most of the homes in the city are owner-occupied, single-family units. Another thing that makes Grayson great for families is that it boasts an impressively high percentage of college-educated adults.
Grayson offers numerous parks, sports programs, shops and restaurants for having a great time outdoors. It also boasts many family-friendly events, such as festivals and concerts. One of the popular events held yearly at Grayson City Park in the heart of the community is Grayson Day, which inspired by Snellville Days in neighboring Snellville. The event, which is held last Saturday in April, features a lively parade down Main Street alongside live music, crafts and superb dining options.
It is without doubt that Grayson is a city to live, work or play. The community boasts superb amenities that make it both a family-friendly and sophisticated professional city. It offers an impressive range of recreational options. You enjoy a small town ambiance while also having convenient access to the big city.


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(E) The R111P mutant eliminates a salt bridge with E114 and produces a clash with the adjacent H110 backbone. the strategy used to display spontaneous suppressor mutations. Download FIG?S2, PDF file, 0.7 MB. Copyright ? 2020 Brzozowski et al. This content is definitely distributed under the terms of the Creative Commons Attribution 4.0 International license. FIG?S3. Analysis of extragenic suppressor mutation. (A) OPC-28326 Pairwise positioning of the sequence in WT (PY79) and the extragenic suppressor (RBSS6E11). The source of 10-nucleotide duplication is definitely highlighted. (B) Growth curves of WT (PY79), (RB314), + (RB409; cultivated in 250 M IPTG), (RB420), and + (RB433; cultivated in 250 M IPTG) are demonstrated. (C) Spot titer assay of WT (PY79), OPC-28326 (RB42), (RB314), (RB420), or strains comprising an inducible copy of in either a WT background (GG82; YpsA) or in a background (RB433). Dilutions of standardized cultures were noticed on solid medium without inducer (remaining panel) or comprising 1 mM IPTG (right panel). Download FIG?S3, PDF file, 2.0 MB. Copyright ? 2020 Brzozowski et al. This content is definitely distributed under the terms of the Creative Commons Attribution 4.0 International license. TABLE?S1. Strains and oligonucleotides used in this study. Download Table?S1, PDF file, 0.1 MB. Copyright ? 2020 Brzozowski et al. This content is definitely distributed under the terms of the Creative Commons Attribution 4.0 International license. ABSTRACT Although many bacterial cell division factors have been uncovered over the years, evidence from recent studies points to the living of yet-to-be-discovered factors involved in cell division regulation. Thus, it is important to identify factors and conditions that regulate cell division to obtain a better understanding of this fundamental biological process. We recently reported that in the Gram-positive organisms and (E55D, P79L, R111P, and G132E) that rendered the mutated YpsA nontoxic upon overproduction. We also isolated an extragenic suppressor mutation in were unable to undergo filamentation in response to Rabbit Polyclonal to DIDO1 YpsA overproduction. We also serendipitously discovered that YfhS may play a role in cell size rules. Finally, we provide evidence showing a mechanistic link between YpsA and YfhS. IMPORTANCE is a rod-shaped Gram-positive model organism. The factors fundamental to the maintenance of cell shape and cell division are of major interest. We display that improved manifestation of results in cell division inhibition and impairment of colony formation on solid medium. Colonies that do arise possess compensatory suppressor mutations. We have isolated multiple intragenic (within knockout mutant is definitely abolished inside a strain that also lacks and (5, 6). These findings highlight the need to investigate and discover other factors involved in regulating cell division in bacteria. In our lab, we have recognized a potential cell division regulator in and phylum of Gram-positive bacteria and appears to be inside OPC-28326 a syntenous relationship having a known cell division protein, GpsB (7). The crystal structure of YpsA was resolved by a structural genomics group in 2006 (PDB ID 2NX2) (8). Based on the structural features, YpsA was placed as the founding member of the YpsA appropriate subclade within the SLOG (SMF/DprA/is definitely 2.79?? [PDB ID 4LJR]), another member of the SLOG superfamily, which is a single-stranded DNA-binding protein involved in DNA recombination (10, 11). Previously, we found that YpsA provides oxidative stress protection in and that overproduction of YpsA results in cell division inhibition, through FtsZ mislocalization, in a growth rate-dependent manner (7). We showed the YpsA-GFP fusion is definitely practical and forms intracellular foci. Focus formation appears to be a prerequisite for filamentation; however, its physiological significance remains unknown. In addition, using site-directed mutagenesis, we recognized multiple amino acid residues that are potentially important for the structure OPC-28326 and/or function of YpsA, including residues located in the conserved substrate binding OPC-28326 pocket made up of glycine and glutamate residues expected by Burroughs et al. (9). In addition, we have demonstrated the potential function of YpsA in cell division is also conserved in the Gram-positive pathogen (7). In this study, we utilized a classic spontaneous.
Organic killer (NK) cells certainly are a specialised population of innate lymphoid cells (ILCs) that help control regional immune system responses. cells). 119 Although tests showed NK\mediated lysis of arthritogenic Tfh and Th17 cells, 119 this might not reveal a physiological situation because other immune system checkpoints (i.e. T regulatory cells) are absent. IFN\ made by NK cells is normally considered to inhibit joint disease also, both in the unaggressive transfer autoantibody\induced joint disease 120 as well as the autoimmune CIA 100 versions. Nevertheless, NK\mediated inhibition of unaggressive transfer autoantibody\induced joint disease is only obvious following CpG\oligonucleotide arousal. 120 Within the CIA model, NK\produced IFN\ is normally considered to limit Th17 differentiation as NK cells depletion with anti\asialoGM1 at priming stage resulted in the extension of Th17 cells and mild exacerbation of CIA at disease starting point. 100 A far more suffered exacerbation of CIA was observed following anti\NK1 similarly.1\mediated depletion, but this is apparently because of NK T cell depletion as Compact disc1dC/C (NK T\lacking) mice also develop worse CIA. 121 Abundant NK cells can be found in RA synovium & most harbour a distinctive CD56bcorrect phenotype. 25 , 26 , 115 , 122 RA synovial NK cells are Compact disc69+ NKp44+, indicative of the activated condition, but are perforinlow. 25 , 26 , 115 , 122 They upregulate surface area appearance of inhibitory Compact disc94\NKG2A also, 123 which highly inhibits NK cell creation of IFN\ and TNF and in addition restrains cytotoxicity upon binding to its ligand, HLA\E. 25 , 119 , 122 Unlike circulating NK cells, synovial NK cells possess low KIR appearance, 25 , 26 , 81 but exhibit chemokine receptors, such as for example CCR5, CCR1 and CXCR3, which may assist in their preferential recruitment into RA synovium. 26 , 81 Provided the reduced cytotoxicity and IFN\ creation of synovial NK cells, 115 these NK cells most likely contribute to regional joint irritation by producing various other pro\inflammatory mediators. Reciprocal activation of joint\infiltrating Compact disc56bcorrect NK cells and Compact disc14+ inflammatory monocytes in addition has been recommended in RA. 25 , 124 Murine research recognize joint NK cells as resources of M\CSF and RANKL that promote the differentiation of bone tissue\resorbing osteoclasts 89 (Amount?1a). As opposed to previously studies, 100 the depletion of NK cells using anti\asialoGM1 attenuated both joint bone tissue and inflammation erosion 5-TAMRA within the CIA model. 89 These scholarly research demonstrate the restrictions of antibody depletion of NK, 125 , 126 which may be further confounded with the dynamics of autoimmune replies. 5-TAMRA Open in another window Amount 1 NK cell participation in autoimmune inflammatory illnesses. NK cells exacerbate RA by secreting soluble mediators such as for example (a) M\CSF and RANKL that get the differentiation of bone tissue\eroding osteoclasts and (b) GM\CSF that stimulates the creation of pro\inflammatory mediators by joint\infiltrating neutrophils. (c) NK cells usually do not may actually play a prominent function in MS but enhancing their cytotoxic function with anti\NKG2A may remove encephalitogenic Th17 cells 5-TAMRA and relieve disease within the EAE model. (d) NK cells may promote SLE through their connections with pDCs via LFA\1 and DNAM\1 that enhances the creation of cytokines and chemokines such as for example IFN\, IFN\, TNF\, IL\6, IL\8, CCL3 and CCL4. NK cells may also be within kidney of lupus nephritis sufferers but it continues to be unclear if NK cells and their cytokine dysfunction donate to tissues pathology. (e) NK cells could donate to the era of autoantigens through extreme eliminating of CV\B4\contaminated pancreatic islets. Nevertheless, other features of NK cells such as for example IFN\ production Anxa1 stay unclear and upcoming studies must catch phenotypic and useful variety of NK cells both in CV\B4\linked and sterile T1DM subtypes. (f) Alveolar NK cells are believed to provide rise to autoantigens such as for example histidyl tRNA synthetase pursuing respiratory insults in anti\synthetase symptoms. Future studies.
The findings of this study indicated that TalaA could be a new potential powerful drug candidate for colorectal cancer therapy due to its outstanding ability to kill colorectal cancer cells via ferroptosis induction. inhabiting were also upregulated by TalaA treatment. ROS is ubiquitous in living organisms23. reactive oxygen species levels to a certain threshold, the exceeding of which induced ferroptosis. On the other hand, this compound downregulated the expression of the channel protein solute carrier family 7 member 11 (SLC7A11) but upregulated arachidonate lipoxygenase 3 (ALOXE3), promoting ferroptosis. Furthermore, in vivo experiments in mice evidenced that TalaA effectively suppressed the growth of xenografted colorectal cancer cells without obvious liver and kidney toxicities. The findings of this study indicated that TalaA could be a new potential powerful drug candidate for colorectal cancer therapy due to its outstanding ability to kill colorectal cancer cells via ferroptosis induction. inhabiting were also upregulated by TalaA treatment. ROS is ubiquitous in living organisms23. It is not only a product of normal cell physiological activities, but also an important NADP signaling molecule24. The growth rate and ROS levels in healthy cells of normal tissues are usually low. However, in cancer cells, ROS production is increased due to the vigorous cell metabolism and proliferation. Meanwhile, a set of antioxidant systems against ROS is Mouse monoclonal to DDR2 derived by cancer cells to prevent themselves from damage caused by ROS; moreover, they can utilize ROS as a positive regulatory signal for advanced survival and proliferation25. When the oxidative stress in cells, caused by ROS, is too strong, they enter programmed death pathways, such as apoptosis and ferroptosis. Of note, cancer cells have higher baseline ROS levels than normal cells. Thus, a strategy to elevate the content of ROS and suppress the activities of antioxidant molecules, which induces cancer cell death, would be a highly sensible strategy for cancer treatment. Notably, in this study we found TalaA strongly elevated the ROS level in CRC cells, which was an important reason why TalaA killed cancer cells via ferroptosis. It is worth noting that the anticancer activity of TalaA is significantly higher than that of erastin in killing cancer cells and triggering ferroptosis. TalaA suppresses the growth of CRC NADP cells through two pathways: (1) by elevation of cancer cell ROS level to initiate ferroptosis; (2) by alteration of the expression of ferroptosis-related molecules (e.g., SLC7A11, ALOXE3, GSS, and HMOX1), which accelerates ferroptosis. Due to its high anticancer activity and low toxicity, TalaA could be a powerful potential candidate drug for CRC chemotherapy. This study reveals the anticancer mechanism of TalaA, and provides important experimental evidence that will facilitate the development of novel anticancer drugs. Materials and methods Fermentation, extraction, and isolation The fungus was isolated from the stems of collected in September 2015 from Baoding, Hebei Province, P.R. China. The isolate was identified as by an analysis of the ITS region of the rDNA (GenBank Accession No. “type”:”entrez-nucleotide”,”attrs”:”text”:”KY230505″,”term_id”:”1270117721″,”term_text”:”KY230505″KY230505) and assigned the accession no. XL-025. A voucher specimen was deposited in School of Pharmaceutical Sciences, South-Central University for Nationalities. The fungus was inoculated aseptically into three 500?mL Erlenmeyer flasks each containing 300?mL of potato dextrose broth (PDB), and then cultured at 28?C for 3 days with shaking at 160?rpm to afford the seed culture. The large-scale fermentation was performed into 150 flasks (500?mL), and each flask contained 80?g of rice and 80?mL glucose solution (20?g/L). Then, 5.0?mL of the seed culture was inoculated into each flask and incubated at room temperature for 50 days. The harvested fermentation material NADP was ultrasonically extracted three times with CHCl3/MeOH (1:1, v/v), and the organic solvent was evaporated under reduced pressure to yield a brown residue. The residue was then suspended in H2O and extracted three times with NADP an equal volume of ethyl acetate (EtOAc) to yield 70?g of crude extract. The EtOAc extract was subjected to a silica gel column chromatography (CC) with a gradient mixture of CH2Cl2/MeOH (100:1C0:1) to afford eight fractions (Fr. ACFr. H). Fraction C (1.2?g) was.
Supplementary Materialsoncotarget-08-91841-s001. inhibitor. Blood sugar rate of metabolism was hampered from the FGFR inhibitors also under hypoxic conditions, with consequent inhibition of cell proliferation and viability. In presence of serum, glucose rate of metabolism was impaired only in cell models in which FGFR1 inhibition was associated with AKT/mTOR down-regulation. When the activation of the AKT/mTOR pathway persisted despite FGFR1 down-regulation, the effectiveness of NVP-BGJ398 could be significantly improved from the combination with NVP-BEZ235 or additional inhibitors of this Olaquindox signaling cascade, both and in xenotransplanted nude mice. Collectively our results show that inhibition of FGFR1 signaling effects on malignancy cell growth also by influencing glucose energy metabolism. In addition, this study strongly shows that the healing efficiency of FGFR1 concentrating on substances in SQCLC could be applied by mixed remedies tackling on blood sugar fat burning capacity. hybridization (Seafood) evaluation in around 20% of SQCLC [13, 14], although a lesser frequency (9%) surfaced from newer analyses predicated on following era sequencing . Today’s study was made to check out the function of FGFR1 signaling within the legislation of blood sugar energy Olaquindox fat burning capacity in FGFR1 amplified/over-expressing SQCLC versions displaying different patterns of molecular modifications. We showed that FGFR1 handles blood sugar uptake and usage by activating the AKT/mTOR pathway in fact, which is in charge of the induction of HIF-1 and GLUT-1 blood sugar transporter appearance, under both hypoxic and normoxic circumstances. Furthermore, FGFR inhibitors – NVP-BGJ398 and PD173074, with selectivity against FGFRs, and dovitinib (TKI258), concentrating on also Vascular Endothelial Development Aspect Receptors (VEGFRs), Platelet Derived Development Aspect Receptors (PDGFRs), FLT3 and c-Kit  – Sema3d had been proven to exert anti-tumor activity by hampering blood sugar fat burning capacity through AKT/mTOR inhibition. Furthermore, our data claim that the mix Olaquindox of selective FGFR inhibitors with targeted down-regulation of AKT/mTOR signaling pathway and therefore blood sugar utilization could improve the restorative effectiveness of FGFR inhibition both and effects of NVP-BGJ398 and NVP-BEZ235 on LENTI-4 tumor xenograftsLENTI-4 cells were implanted s.c. in BALB/c-Nude mice. Vehicle, NVP-BGJ398 (30 mg/Kg) and NVP-BEZ235 (15 mg/kg) were administered five instances per week by orogastric gavage. (a) Tumor sizes were measured two times per week and data are indicated as percentage of switch in tumor volume SEM of 8 tumors per group. **p 0.01, ****p 0.0001 vs C; #p 0.05, ##p 0.01, ####p 0.0001 vs NVP-BGJ398; $$p 0.01 vs NVP-BEZ235. Inset: representative images of dissected xenograft tumors. (b) Panel Insets: low magnifications of selected examples of Masson’s Trichrome stained sections of subcutaneous LENTI-4 induced tumor xenograft from untreated (C) and drug treated mice. in NVP-BGJ398+NVP-BEZ235 shows a large necrotic area (scale bars: 500m). Representative microscopic images of the same samples are demonstrated at higher magnification on related panels. Intense collagen deposition (greenish) between neoplastic cells (purple) is apparent in NVP-BEZ235 and NVP-BGJ398+NVP-BEZ235 treated xenografts (level bars: 200m). (c) Bar graph illustrating the quantitative measurements of neoplastic, connective and necrotic tissue compartments composing LENTI-4 induced tumor xenografts from untreated (C) and drug treated mice. *p 0.05, **p 0.01 vs C; #p 0.05 vs NVP-BGJ398; $p 0.05 vs NVP-BEZ235. We assessed the real impact of the different pharmacologic treatments on tumor mass by accurate morphometric analysis of tissue composition within the nodule. By this approach, a significant reduction in the fractional volume occupied by neoplastic cells was documented in xenografts after the administration of NVP-BGJ398 (-12.10%) or NVP-BEZ235 (-13.23%) when compared to control group. The simultaneous inhibition of FGFR1 by NVP-BGJ398 and PI3K/mTORC1-C2 by NVP-BEZ235 resulted in a nearly 40% decrease in neoplastic tissue when compared to control group and by 27.7% and 26.8% when compared to individual NVP-BGJ398 or NVP-BEZ235 treatments, respectively (Figure 8b, 8c). Interestingly, as shown by Western Blot analysis performed on tissue tumor extracts, the combination of NVP-BGJ398 and NVP-BEZ235 inhibited the src/FAK signaling pathway, confirming the result obtained (Figure ?(Figure9a).9a). In addition, RT-PCR analysis demonstrated that also GLUT-1 mRNA expression was significantly down-regulated by the combined treatment (Figure ?(Figure9b).9b). The expression of GLUT-1 was also assessed by immunohistochemistry on treated and untreated tumor xenografts. Compared to controls, a significant 46.29% reduction in GLUT-1 positive cells was documented in tumors treated with the combination of NVP-BGJ398 and NVP-BEZ235 while the inhibitory effect of individual drugs did not reach statistical significance (Figure 9c, 9d). Open in a separate window Figure 9 Effects of NVP-BGJ398 and NVP-BEZ235 on src/FAK signaling and GLUT-1 expression in LENTI-4 tumor xenografts(a) Total proteins had been extracted from cells samples from LENTI-4 induced tumor xenografts from control and NVP-BEZ235, NVP-BGJ398 or NVP-BGJ398+NVP-BEZ235 treated BALB/c-Nude mice. European Blotting was performed to judge FAK and src activation/expression. Email address details are representative of two 3rd party tests. (b) Total RNA.
The broad relationship between the immune system and cancer is opening a new hallmark to explore for nanomedicine. stimulating the immune response . Currently, there are some DNA vaccines include mammaglobin-A for breast cancer, PAP for prostate cancer, and gp100 and gp75 DNA for melanoma [136,137,138,139]. Disadvantages may be the method of DNA/RNA delivery and the efficiency of absorption, which may limit transcription and antigenic presentation by APCs . These vaccines have been administered using viral vectors and electroporation, which are effective but difficult to apply in the medical regular [140,141]. It will also be mentioned how the administration of live disease may cause unwanted effects and reduce the performance of antiviral antibodies in individuals . em v. Vaccines focusing on TAAs /em : To accomplish tumour-specific death, tumor vaccines must focus on limited MK-8745 epitopes of MHC-I that activate Compact disc8+ T cells, as they are the strongest cells so when triggered recognize TSAs and distinguish MK-8745 regular cells from cancer cells . This involves the following processes: degradation of ubiquitous proteins by the proteasome, interaction of peptides with Hsp90 in the cytosol, which acts as a chaperone, active transport into the endoplasmic reticulum by the TAP transporter, modification of peptides by ERAP to an appropriate length, which are subsequently loaded into the peptide-binding cleft of MHC class I molecules with the help of chaperones such as tapain and transport to the cell surface, and can thus be recognised by the CD8+ T-cell receptor . There are different types of tumour antigens that can be targeted in immunotherapy: (i) tumour-associated antigens (TAA), which are over-expressed on tumour cells and are expressed to a lesser extent on normal cells, (ii) cancer germ-line antigens (CGA), which on normal adult cells are found only in reproductive tissues, but are expressed selectively on several types of tumours, (iii) virus-associated antigens, which arise in tumour cells from oncogenic viral proteins; and (iv) tumour-specific antigens (TSAs), which are the neo-antigens and are only found in tumour cells, as they arise from non-anonymous somatic mutations . Commonly, cancer vaccines should target the broadest possible antigen repertoire, which can MK-8745 be achieved by using autologous tumour lysates, whole-tumour-derived mRNA, irradiated autologous tumour cells, or allogeneic tumour cell lines [144,145]. In addition, effective responses in response to an antigen can result in the immunogenic release of additional endogenous antigens by tumour cell destruction, leading to a broader immune response. This is known as epitope spread . Vaccines targeting TAAs have not been very successful so far and are still under development, mainly because many TAAs are also expressed on normal cells, which show central and peripheral tolerance, MK-8745 and the affinity of TCR for these antigens might be very low . In addition, autoimmune toxicities may take place during treatment. Despite this, some AATs are used as targets Despite the weak points on this approach; Currently, several approaches has been quite promising and help to open more studies exploring the full potential, for example: CD19-directed CAR-T therapy in acute lymphoblastic leukemia (ALL), which leads to full remission in a lot of individuals . CGAs, such as for example melanoma connected antigen 3 (MAGE-A3) and NY-ESO-1 antigen, are indicated in a few malignancies selectively, but when utilized as a focus on they bring about high toxicities. Specifically, serious neurological loss of life and toxicities occur when MAGE-A3 is targeted . Alternatively, virus-coded antigens are just present on tumour cells, not really on regular cells, as some malignancies are connected with pathogen disease. Viral oncogenes encode oncoproteins that trigger cell transformation. A good example is the human being papilloma pathogen Rabbit Polyclonal to MRPS31 (HPV), which can be connected with cervical tumor . This technique continues to be effective in dealing with cancer, but there’s also virus-associated antigens with the ability to escape from the immune system . In the approach of these vaccines, the critical and important key aspect is the selection of tumour-specific antigens (TSA), which are the neo-antigens. These are peptides that arise from non-anonymous mutations, alterations in genomic codons, editing, processing and antigen presentation in tumour cells . Among all non-synonymous mutations, a part of them is distributed clonally by the tumour and generates peptides containing mutations (neo-epitopes) that can be recognised by cytotoxic T cells. Deletions and insertions are highly predictive of response  also. The usage of these mutant produced epitopes is dependant on the replies to checkpoint inhibitors primarily, that are proportional towards the mutational fill of every tumour . Neoantigens are shown by MHC in the cell surface area to become recognised by.
Background The purpose of this study was to explore the consequences from the Na+/K+ ATPase inhibitor ouabain in regulating osteosarcoma (OS) cell stemness. stemness. Additionally, ouabain enhances cisplatin awareness of Operating-system cells, which is involved with Ca2+ DNA and channel methylation. Conclusions This ongoing function offers a potential substance for dealing with Operating-system sufferers, operating-system sufferers with chemoresistance specifically. check was employed for examining the datasets with just 2 groups. Distinctions between groups had been examined using one-way ANOVA using the Tukey-Kramer post hoc check. P value significantly less than 0.05 was considered significant. Outcomes Ouabain reduces Operating-system cell stemness but provides little influence on cell viability We initial investigated the consequences of ouabain on Operating-system cell stemness and discovered that ouabain considerably reduced ALDH1 activity in Operating-system cells within a concentration-dependent way (Amount 1A). Furthermore, the appearance of vital stemness regulators (Oct4, sox2 and Nanog) was reduced by ouabain in Operating-system cells (Amount 1BC1E). Additionally, both sphere size and amount were decreased by ouabain (Amount 1F, 1G). Notably, the viability of Operating-system cells somewhat was reduced, however the difference had not been significant (Amount 1H, 1I). These total results demonstrate that ouabain can reduce OS cell stemness. Open in another window Amount 1 Ouabain decreases Operating-system cell stemness but offers little effects on cell viability. (A) The activity of ALDH1 was measured in OS cells treated with different concentrations of ouabain. (BCD) QPCR analysis within the mRNA levels of essential stemness regulators in OS cells with ouabain treatment. (E) European blot analysis of the protein levels of essential stemness Muscimol regulators in OS cells treated with ouabain. (F) Sphere size was evaluated in OS cells with ouabain treatment. (G) Sphere quantity was identified in OS cells with ouabain treatment. (H, I) Cell viability was assessed in OS cells with ouabain treatment. ** P 0.01 control. Ouabain inhibits OS cell migration, invasion, and adhesion ability Since stem-like cells result in tumor cell metastasis, we further examined the effects of ouabain on OS cell migration and invasion. OS cell migration, invasion, and cell adhesion capabilities were reduced by ouabain treatment (Number 2AC2E). Open in a separate window Number 2 Ouabain inhibits OS cell migration, invasion, and adhesion ability. (A, B) The migration ability was evaluated in OS cells with ouabain treatment. (C, D) The invasion ability was identified in OS cells with ouabain treatment. (E) Cell adhesion capacity was measured in OS cells treated with ouabain. * P 0.05, ** P 0.01 control. Ouabain prospects to DNA methylation of stemness markers through increasing intracellular Ca2+ concentration DNA methylation takes on a critical part in regulating gene manifestation. A recent study found that inhibition of Na+/K+ ATPase and intracellular increase of Mmp13 calcium level negatively affects the stemness of circulating tumor cells . Consequently, we hypothesized the intracellular Ca2+ concentration and its mediated DNA methylation are involved in ouabain-mediated regulation of the manifestation of stemness regulators (Oct4, sox2, and Nanog). We tested this by Muscimol Muscimol measuring the intracellular Ca2+ concentration and methylation level of the essential stemness regulators and found that the intracellular Ca2+ concentration and Muscimol methylation level of the essential stemness regulators were higher in OS cells than in OS cells-formed spheres, which exhibit stem cell-like traits  (Figure 3A, 3B). As expected, the methylation level of stemness regulators was remarkably increased in OS cells treated with ouabain (Figure 3C). To gain more insights into the mechanisms contributing to ouabain in regulating OS cell stemness, we tested whether ouabain regulates calcium level in OS cells, finding that the intracellular Ca2+ level was increased.
Data Availability StatementNot applicable. of SPOP in tumorigenesis predicated on three major categories: physiological evidence (animal models), pathological AZD5363 kinase inhibitor evidence (human malignancy specimens) and biochemical evidence (downstream ubiquitin substrates). Furthermore, we note that SPOP could be a promising therapeutic focus on for cancers treatment. (mice passed away between embryonic time 18.5 and postnatal time 1 . Blattner et al. built a prostate-specific SPOP-F133?V mutation-carrying transgenic mouse and discovered that PrCa originated in part because of the activation from the PI3K/mTOR and AR signaling pathways aswell as the increased loss of . Additionally, the PrCa-derived SPOP-F133?V mutation selectively damaged the homology-directed fix function mediated by wild-type SPOP (wt-SPOP) . Clinical data demonstrated that SPOP downregulation and mutations had been discovered in individual PrCa tissue, and these mutations had been also correlated with a worse prognosis in sufferers with PrCa  tightly. More importantly, comprehensive biochemical evidence provides further indicated that SPOP functions as a tumor suppressor by promoting the degradation of oncogenic substrates in PrCa, including SRC3 , AR , TRIM24 , c-Myc , DEK , SENP7 , EglN2 , ATF2 , Cdc20 , ERG [71, 81], BRD4 [72C74], PD-L1  and cyclin E1 . Due to Rabbit Polyclonal to FPRL2 the many publications and space limitations, we will not describe the tumor suppressive role that SPOP plays by promoting the ubiquitination and degradation of its substrates in PrCa in detail. Therefore, we sincerely apologize to some experts for not citing their important and meaningful papers. Lung malignancy (LC)LC is one of the leading causes of cancer-related death in the world . Downregulation of SPOP has been observed in non-small cell LC (NSCLC) tissues compared with normal tissues at both the transcriptional and translational levels . Furthermore, the level of SPOP was confirmed to be associated with several clinicopathologic parameters, and a decrease in SPOP was considered a predictor of poor prognosis in patients with NSCLC, suggesting that SPOP could be a potential tumor suppressor in LC . The sirtuin (SIRT) family of NAD-dependent protein lysine deacylases has been reported to participate in multiple biological processes such as transcription regulation, metabolism and DNA repair [84C86]. Notably, one group showed that SPOP promoted the proteasomal degradation of SIRT2 by binding to it, thus suppressing the growth of NSCLC cells . Moreover, this ability was inhibited by mutation of SPOP in NSCLC cells. Furthermore, compared with the normal cells, NSCLC cell lines experienced elevated SIRT2 and reduced SPOP levels . Fas-associated protein with death domain name (FADD) is the important adaptor protein that transmits extrinsic apoptotic cell death signals by recruiting complexes of caspase 8 to loss of life receptors [87, 88]. Rising evidence in addition has proven that FADD expression is certainly involved with cancer and tumorigenesis progression. For example, overexpression of FADD may serve seeing that a biomarker in throat and mind squamous cell carcinoma . Furthermore, a higher degree of FADD proteins continues to be reported to become connected with poor final result in LC also, suggesting that it might become a powerful prognostic biomarker in LC sufferers [48, 90]. Luo et al. discovered that SPOP bound to FADD and marketed its ubiquitination and degradation straight, blocking the introduction of NSCLC . As a result, SPOP exerts anticancer results by concentrating on FADD in LC. Oddly enough, an AZD5363 kinase inhibitor oncogenic function of SPOP in LC continues to be indicated recently  also. SPOP was present to become expressed in various LC cell lines widely. Conversely, knockdown of by shRNA in LC cells resulted in DNA damage fix defects, elevated cell sensitization and apoptosis to irradiation AZD5363 kinase inhibitor in DNA harm conditions . As a result, in-depth investigation is vital to look for the function of SPOP in LC. Gastric cancers (GC)GC is among the leading factors behind cancer-related death world-wide and has a poor response to current chemotherapy . The sonic hedgehog (Shh) signaling pathway is crucial for growth control and patterning during embryonic development.

domain: medical


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'Maybe': Tuchel offers possible problem solver that could benefit Chelsea and other PL clubs
Chelsea manager Thomas Tuchel has suggested international fixtures across the board should be moved to green-listed countries.
Speaking in his latest press conference, Tuchel was predictably asked about the furore surrounding FIFA rulings on players called up to represent their country but held back by their clubs.
Thiago Silva faces not being allowed to feature for Chelsea because of a FIFA-enforced six-day ban.
South American stars from Liverpool, Manchester City, Manchester United and Leeds also face the prospect of missing the weekend’s Premier League action.
Understandably, the clubs detained their players to protect them from entering a UK red-listed country and subsequently quarantining after their international games had concluded.
Tottenham Hotspur, Aston Villa, Watford and Wolverhampton Wanderers could also be affected.
Trialling Tuchel’s idea
To avoid such a debacle happening again, Tuchel offered an alternative to the current schedule on offer to Latin American nations.
“For me, personally, maybe a solution is we move the games from red-listed countries to green-listed countries, like we did with the Champions League,” said the Chelsea manager.
“We played in Romania, we played in Seville. This was a solution where games could be played. Maybe we can find a solution like this?”
Tuchel went on to condemn the decision by FIFA to punish players who didn’t even travel away.
“Of course the clubs want their players to play, the national teams as well. The players do as well, don’t forget it.
“Still, if it ends up in a situation where players can’t play for the national team and then they are suspended in their league, it doesn’t help anybody.
“That makes no sense. Not for the fans, not for the football, not for the national team, and not for the clubs.”
The Blues boss did reveal Silva’s injunction had yet to be officially finalised and he was waiting on confirmation from Chelsea chairman Bruce Buck.
Having the defender back would be a big boost, especially as Chelsea play on Saturday in the league and again on Tuesday in the Champions League.
Liverpool and Manchester City will hope for similarly positive news as they face being without several key men for their respective matches.

domain: news


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One of the biggest surprises of Square Enix’s Collaboration with Gumi in Final Fantasy Brave Exvius is the ability to fight Brave Frontier’s Maxwell.
In just the span of one week, players of Final Fantasy Brave Exvius can challenge the notorious Maxwell and earn rewards for beating her. Thanks to the newly introduced Mission system, there are valuable rewards waiting for everyone, though there are certain conditions to be met. Here are the rewards for beating Maxwell.
4* Trust Moogle
Condition: Complete the quest by defeating Maxwell
Condition: Defeat Maxwell by using a Limit Break
15% Dark Resistance Materia
Condition: Defeat Maxwell without not having anyone die in battle
Maxwell (5* Unit)
Condition: Evoke Diabolos
Maxwell in this week’s event is challenging. Those who don’t have units with TMR will be having a hard time and will require a lot of patience. In my case, my units are the following:
From the units above, about 50-75% are equipped with TMRs. It was an average fight for me as it took me about 3 turns to beat her – that’s without trying to evoke Diabolos to get Maxwell.
Now, you want to beat her? Here’s our tips!
Final Final Brave Exvius
Must Have Units
– Tank (specifically Cecil is the best option for this battle as it can do both damage mitigation and heal. If you have Dual Cast equipped on him, the better)
– Cloud of Darkness 6* (Her Omni Veil helps all units have resistance on her Light attacks. The Barrage Skill is also useful as it help builds up the Esper Evoke Bar.
– Refia 6* (Her party buff is a must in order for units to deal more damage than normal. Also her Revive skill is quite useful as most likely units will die in this fight)
Genesis – AOE + ATK Down
Meteor – ST + DEF Down
Destiny – Powerful ST Damage with chance of reduce Max HP (every 5 turns)
Resurrection – HP Recovery
Endless – Powerful AOE Attack
Revive – HP Recovery (120,000)
– During the first turn, Maxwell will use Genesis that deals AOE damage, as well debuffing your units with attack down. Make sure that your Cecil uses Provoke on this turn.
– Have the rest of your units do the necessary party buffs during the first time. If you have Ramza, do the Hero’s Song or Refia’s Embolden
– Maxwell can be debuffed (Attack/Def/SPR/Mag Down). If you have an Elza friend, her Ravaging Blow will be useful in this fight. If not, Full Break will work.
– Maxwell is weak against Dark element attacks. That means, Cloud of Darkness’ Call of the Void will be very useful in this fight.
– You will need to Evoke Diabolos in battle to get Maxwell.
– If you have Excalibur or any light property weapons, unequip it! Maxwell will resist it.
– Keep your party buff and make sure you have Curaja as there are times Maxwell will unleash several AOE attacks.
– Barrage is a useful TMR in this battle as it will help you evoke Diabolos faster
– Since Maxwell is a human, Man-Eater passive skill helps deal more damage

domain: game


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A male aged 25 from chadiza district committed suicide after receiving news that hewas HIV positive. The incidence happened when Daka fell off the bike and injured himself. Upon arrival he was told of the mandatory HIV testing.
Unfortunately the results came out positive. They commenced him on instant initiation of treatment and was given a two weeks course of ARVs upon reaching home because he was still in denial Daka took all the medicines and died.
The hospital has few counsellors and there is health staff shortage as a result of mandatory testing . This has increased workload especially on nurses Biomedical Technicians carrying out tests.


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Alfie series - Alfie in the Snow (Alfie series, Book 5)
Can Alfie and George save Christmas?
The Sunday Times bestseller returns for a fifth book! Alfie and his mischievous kitten George are back for more adventures
When a new family moves onto Edgar Road, Alfie and George can’t wait to welcome them – especially when they discover they have a cat too!
Alfie imagines they’ll be the perfect neighbours, but when one little misunderstanding gets blown out of proportion, the street is suddenly at war!
As the snow starts to fall, Alfie and George have their work cut out for them to bring the street back together. But can one doorstep cat and his mischievous kitten use their festive spirit to reunite everyone in time for Christmas?
A heart-warming novel that is perfect to curl up with this festive season. For fans of A Street Cat Named Bob, Cathy Bramley and Holly Hepburn.
Praise for Rachel Wells: -
”'Must have!” - Daily Express
”'The first book, 'Alfie the Doorstep Cat” - was absolutely brilliant - and now this follow up is even better.' Reader review
”'A must for all cat lovers.” - Reader review
”'I loved this book! Loved the first book 'Alfie the Doorstop Cat' and this one was just as good as the first. I couldn’t put it down. Great for a cat lover to read!” - Reader review

domain: library


South Carolina recently passed a law that requires voters to show a photo ID when they go to the polls, and we have said all along those who object to such a move are throwing a red herring into the equation. There’s certainly nothing wrong with having people prove who they say they are when they go to exercise the basic right of democracy, and the photo ID law does nothing to prevent people from voting or making it more difficult for them to do so.
But many states are now attempting to throw on additional voting requirements, and there is reason for concern. Many of these changes are being proposed in states that have newly elected Republican-controlled legislatures, and Democrats, of course, are crying foul. But the fact is that Democrats, when they have controlled legislatures, have pursued rules changes of their own. Both parties are continually trying to move into position to get more of their own voters to the ballot box.
Several states are moving to limit the number of days in which people can vote early. Democrats claim that favors Republicans because it’s easier for them to get to the polls, but we’re not sure we’re buying that argument. And other states are considering changes they think make it easier for special-interest groups to conduct voter registration drives and get people to the polls.
The bottom line is that this type of movement is just one more indication of how the two major parties in this country are continually jousting in an effort to gain an advantage. The problem is that voting in a reasonable manner should be available to everyone in this country and shouldn’t be subject to the whims of ambitious political professionals. Americans should be able to go to the polls in a reasonable manner and cast their ballots for the people they want to be their leaders, and political posturing should play no part in the process.


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QC Chemist Jobs in New Jersey
Lab Support is currently recruiting for a Junior QC Chemist to work in a global fragrance and flavors company. The qualified candidate will be working in a fragrance manufacturing environment performing QC analysis of aroma chemicals, essential oils and complex fragrance compounds. This position is available on a contract to hire basis. Job Responsibilities: Proficiency in utilizing GC, GC-MS, Fla...
SPEX CertiPrep Group, LLC, a leading manufacturer of organic analytical calibration standards located in Central NJ is seeking candidates. We are committed to providing analytical chemists with the highest quality calibration standards for Gas Chromatography, Gas Chromatography/Mass Spectrometry, Liquid Chromatography/Mass Spectrometry, and High Pressure Liquid Chromatography. The Organic QC Chemi...
Come grow with us! At Laureate Biopharmaceutical Services in Princeton, NJ, you’ll have the opportunity to work alongside some of the most impressive thought leaders in the industry, in a stimulating and enriching work environment. We are seeking a Sr. QC Chemist to join our Quality Control team. In this role the successful candidate will conduct routine and non-routine analysis of raw materials,...
Currently we have temporary to possible permanent opening for an Organic Chemist to support an Electronic Materials company located in Central NJ. Requirements: PhD in organic chemistry Experience in Synthesis Experience with characterization, Nanotechnology Carbon experience; Carbon nanotubes (CNT) and (GNR) Graphene nanoribbons Responsibilities: Goal is to develop to new carbon materials, nanori...
REPORTING RELATIONSHIP: Reports to the Quality Control Supervisor Job Description The Metal Finishing QC Specialist is a dual role consisting of maintaining and tracking he chemistry of the metal finishing operations and being responsible for the inspection of parts processed through the metal finishing operation. The Metal Finishing QC Specialistreports directly to the Medin QC Manager for inspec...
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Which master's degree is better to pursue; Materials Science, Chemical Engineering or Bioengineering...
I have a bs in chemistry and ba in physics. I'm going to apply to med school, i just dont want to do post-bacc program (GPA=3.4). If i dont make it to med school, i would like a better job option than...
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I finished M.Sc. Chemistry in INDIA and got one year experienced in Pharmaceutical company as QC Chemist.Then finished MBA from USA in 2009.But now i dont know what are the options for me to get job i...
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Which is better job in a pharma industry among production chemist and QC/QA i.e, analytical chemist?
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$32,000.00 - $90,000.00
Typical Salary for Chemist in New Jersey
Source: Monster.com Careerbenchmarking Tool
Education / Training
Source: Monster.com Careerbenchmarking Tool
Develops chemical products and processes by conducting research, analysis, synthesis, and experimentation; conducts qualitative and quantitative chemical analysis to develop new products or knowledge.
Rate of Growth
Size of Industry in 2006:
Source: Bureau of Labor Statistics, May 2006
Clinical Lab Testing
Determines process standards by developing formulas and methods.
Researches new products and processes by conducting experiments involving the composition, structure, properties, relationships, and reactions of matter induced by heat, light, energy, and chemical catalysts.
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Sample Resume for an Entry-Level Chemist
Does your resume lack substance? View this resume sample for an entry-level chemist to see how you can formulate a resume to help launch your career.
Sample Resume for a Midlevel Chemist
Help employers bond with your candidacy by creating a resume that emphasizes results. Get ideas from this sample resume for a midlevel chemist.

domain: chemistry


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You're browsing the GameFAQs Message Boards as a guest. Sign Up for free (or Log In if you already have an account) to be able to post messages, change how messages are displayed, and view media in posts.
I bought the game a couple weeks after launch, and I spent most of my first 5 pretiges just unlocking attachments for every gun, so I'm just now getting into the whole gold/diamond camo business.
I'm working on the Skorpion, and it just doesn't ever get headshots. With the MP7 I could hipfire and aim vaguely towards the head and get headshots at a decent rate, but no dice with the skorpion. It's like one headshot every 3 games, typically with 15-40 kills per game depending on what game type.
If I recall the Skorpion has a low/nonexistent headshot multiplier, so I'm guessing that's the culprit. Getting a headshot doesn't count for extra damage and thus have a higher chance of being the killing blow, but still. It's going to suck using this gun for like a week.
I suck at getting headshots for those challenges. I usually get one headshot at most per game. Sometimes I'll go the entire match without a headshot. I was also trying to do the Scorpion challenges, so I averaged like 10 headshots per day. Finally when I got to 100, I decided to switch over to the FAL as my main weapon before I got the Gold camo. <_____<
I dunno, a lot of times I feel like I'm aiming for the head area and I still won't get the headshot. Sometimes I'll even get free shots at snipers standing still and don't see me behind them, and I still don't get the headshot even when I was sure I shot them in the head. It was really frustrating.
I guess my advice would be to take it slow, and don't try to do too much in one day. Concussions definitely help get easy headshots, so run concussions with Scavenger so you can replenish a concussion with every kill. Definitely be sure to take advantage of those guys standing still who don't notice you sneaking up on them.
Wait I got it! Super Mario 3D Land will be in California and Super Mario 3D World will be in Florida. Zing! ~AloofGuyXXVII


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Through 12 Oct by Sam Kuma
Current Price: $0.00
About the book
Do you want to become vegan but concerned about a diet with lack of protein? Are you just looking for vegan protein sources?
Then, read below:
The vegan diet is easily one of the healthiest diets in the world and for good reason. Not only does it help in cutting down on the existing fat in your body but also enhances your disease fighting capacity. It is designed to provide people with a complete solution to their weight loss issues and aid with the slimming down process.
However, when people hear of Vegan diet, they always ask, ‘where do I get my protein intake from?’ but the fact is plant-based products have ample proteins, and if you cook your food with the right ingredients – plant based proteins are more than enough to build a lean body.
This book is designed so that you can follow a protein-rich vegan diet without having to struggle for new recipes. The recipes in this book are easy to cook, 100% vegan and rich in protein. These recipes are designed for both novice and expert cooks and make use of all the best vegan ingredients that one can buy from the market.
The diet incorporates the use of protein rich foods such as chickpeas, quinoa, soya and rice bran, which help in building lean muscles. Below is a summary of the different recipe types we have listed:
High Protein Vegan SmoothiesHigh Protein Vegan SaladsHigh Protein Vegan SoupsHigh Protein Vegan DesertsHigh Protein Vegan Stir FryBonus: Vegan Lifestyle book Included
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Available on: Amazon
Health and Fitness

domain: food