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3b), suggesting how the mAb is directed to TCR. MW under reducing circumstances from peripheral bloodstream T lymphocytes lysed in nonidet P\40 buffer, whilst it precipitated the Compact disc3CTCR complex through the cells lysed in digitonin. Additional evaluation exposed that almost all was identified by the antibody, however, not all, from the T cells, recommending that there could be more isotypes of TCR than thought presently. The antibody was struggling to stimulate T cells to proliferate em in vitro /em , recommending these cells are triggered with a different activation system from that of T cells. Intro The T\cell receptor (TCR) can be a heterodimeric surface area protein from the Compact disc3 subcomplex to create the Compact disc3CTCR complex, which mediates antigen recognition and following sign activation and transduction of immunocompetent T lymphocytes.1 Hitherto, two types of TCR have already been identified, the TCR, formed by \chains and \ as well as the TCR, shaped by \chains and \. In humans and rodents, most T cells communicate the TCR (termed T cells) whilst a small amount of T cells carry TCR ( T cells). On the other hand, in pigs and ruminants, there are always a huge percentage of circulating T cells. Lately, it is becoming very clear that T cells will vary from PYZD-4409 T cells in several essential respects strikingly, such as for example their early appearance in ontogeny,2 limited V gene utilization but intensive junctional variety,2,3 immediate reputation of antigens without antigen digesting,3 exclusive cells and trafficking distribution,2,4 aswell while executing some features not linked to antigen reputation directly.5C10 Each one of these differences indicate that T cells donate to immune system competence in a distinctive way which continues to be poorly understood. The pig, using its abundant circulating T\cell inhabitants, can be a good model for research from the features and character of the T\cell subset. Although three types of porcine TCR have already been determined by molecular and biochemical natural strategies,11C13 particular monoclonal antibodies (mAb) remain lacking to recognize directly, and study therefore, these T cells and their receptors. Although many mAb putatively knowing porcine TCR have already PYZD-4409 been reported in the next international Compact disc workshop,14 these mAb possess yet to become characterized fully. To be able to address this presssing concern, a mAb continues to be produced by us aimed to porcine TCR and right here record its cells distribution, biochemical lack and property of mitogenic effect. Components and strategies Pets and antibodiesThe pets found in this scholarly research were outbred Good sized White colored pigs of either sex. The next anti\porcine lymphocyte mAbs have already been recorded: anti\Compact disc2, MSA4 [immunoglobulin G2a (IgG2a)];15 anti\CD3, PPT3 (IgG1);16 anti\CD4, 74\12\4 (IgG2b);17 anti\CD8, PPT21 and PPT22 (IgG1);18 and anti\sheep TCR, 86D (IgG1).19 B cells were determined with fluorescein isothiocyanate (FITC)\conjugated goat anti\porcine immunoglobulin (Southern Biotechnology Association, Inc, Birmingham, AL). FITC\ or phycoerythrin\ (PE) labelled goat anti\murine subclass immunoglobulin antibodies and streptavidin\PE/CY.5 were purchased from Southern Biotechnology Association, Inc. Planning of mAbThe immunization elsewhere continues to be described.16 Briefly, 2 106 porcine peripheral blood lymphocytes (PBL) had been injected right into a footpad of the BALB/c mouse at 3\day time intervals. On day time 22, the mice were draining and killed popliteal lymph nodes were removed for fusion. Fusion of cells was completed as referred to before.16 Supernatants of hybrids were tested for his or her binding to PYZD-4409 porcine PBL by Rabbit Polyclonal to p130 Cas (phospho-Tyr410) flow cytometric (FCM) analysis and one mAb PPT27 was chosen for even more characterization. Movement cytometryThis somewhere else was done mainly because described.20 For two\color staining, PBL were treated with an assortment of mAb PPT27 (IgG2b) and anti\Compact disc2 (IgG2a), Compact disc3 (IgG1), anti\skillet\Compact disc8 mAb PPT21 (IgG1), anti\Compact disc8hi there mAb PPT22 (IgG1), or FITC\conjugated anti\pig immunoglobulin, accompanied by incubation with an assortment of PE\conjugated anti\mouse IgG2b and either FITC\anti\mouse IgG2a, FITC\anti\mouse IgG1, or basic buffer. For co\staining with anti\Compact disc4, the cells had been incubated with PPT27 1st, accompanied by PE\anti\mouse IgG2b, clogged with 10% regular mouse serum and lastly stained with biotinylated anti\Compact disc4 accompanied by FITC\streptavidin. 3\color staining was conducted while described. 20 glycosidase and Immunoprecipitation treatmentsIodination and immunoprecipitation had been completed following methods as previously referred to.16 Glycosidase digestion of iodinated antigen precipitated from the protein G beads (Sigma Chemical substance Co, St Louis, MO) coated with mAb PPT27 was finished with Endo\F and Endo\H (Boehringer Mannheim, Mannheim, Germany) following a producers instructions. Cell parting and lymphocyte proliferationPorcine peripheral bloodstream mononuclear cells (PBMC) had been ready as reported somewhere else.16 Cell subsets were selectively depleted from PBMC using the mini MACS program (Miltenyi Biotec GmbH, 51429 Bergisch Gladbach, Germany) following a producers instructions. Induction of lymphocyte.
Category Archives: Potassium Channels, Non-selective
The supernatant was ultracentrifuged at 130?000?for 30?min as well as the supernatant was retained. Purkinje cells. The testing of the cerebellar\expression library led to the isolation of proteins kinase C (PKC). PKC immunoreactivity had not been seen in the serum of 170 sufferers with non\paraneoplastic neurological syndromes, 27 sufferers with PCD, no onconeural antibodies and little\cell lung cancers, and 52 sufferers with NSCLC without paraneoplastic neurological syndromes. The NSCLC from 11 patients without PCD didn’t express PKC at either the protein or RNA level. Nevertheless, many cells from the NSCLC of the individual with PKC antibodies portrayed PKC. Bottom line PCD takes place in sufferers with NSCLC without regular onconeural antibodies and it is associated with immune system reactions against essential proteins from the Purkinje cells. Paraneoplastic cerebellar degeneration (PCD) is certainly characterised by selective harm to the Purkinje cells from the cerebellum, which in turn causes a serious pancerebellar syndrome usually.1 In sufferers with lung cancers, PCD is nearly always connected with little\cell lung cancers (SCLC).2 Sufferers with non\little\cell lung cancers (NSCLC) and PCD usually harbour onconeural antibodies typically connected with SCLC in the serum and cerebrospinal liquid, which indicate a common immune system\mediated mechanism of neuronal damage most likely.3,4 Although several studies have got indicated the occurrence of PCD in sufferers with NSCLC no onconeural antibodies, these sufferers were usually contained in larger group of sufferers with PCD with different tumours2,5,6,7 or had been reported as solo observations.8 Within this scholarly research, we explain the clinical and immunological findings of some sufferers without previously characterised onconeural antibodies who offered PCD connected with NSCLC. Sufferers and strategies We retrieved from our archives the info on sufferers with the ultimate diagnosis of traditional PCD, regarding to published requirements,9 and NSCLC. We particularly excluded sufferers who had been positive for onconeural antibodies (Hu, Yo, Ri, CV2, Tr, Ma2, amphiphysin). Sufferers with PCD with this profile symbolized just 4% of the complete group of 121 sufferers with PCD signed up in Barcelona’s data source. Serum and cerebrospinal liquid, when available, had been examined by immunohistochemistry, on iced parts of paraformaldehyde\set rat tissue.10 Rat cerebella were homogenised in the current presence of protease inhibitors and centrifuged at 3000?for 10?min. The supernatant was HILDA ultracentrifuged at 130?000?for 30?min as well as the supernatant was retained. Examples had been separated by electrophoresis on the 4C12% polyacrylamide gel, used in nitrocellulose paper and put through regular immunoblot techniques using an BRD-6929 avidinCbiotin technique as defined previously.10 Verification of the cerebellar cDNA expression library A Uni\ZAP XR Library (Stratagene, La Jolla, California, USA) from human cerebellum was immunoscreened as reported previously.11 Phage\positive clones had been subcloned in pBluescript using the in vivo excision phage recovery process (Stratagene) and sequenced. The NCBI BLASTn plan (National Middle for Biotechnology Details, Country wide Institutes of Wellness, Bethesda, Maryland, USA) was utilized to find homologies. Affinity purification of antibodies Filter systems with purified phage plaques expressing proteins kinase C (PKC) or unimportant proteins had been incubated with patient’s serum or an anti\Hu\positive serum (dilution 1:200) for 12?h in 4C. After comprehensive washing, destined antibodies had been eluted with sodium citrate, pH 2.5, and neutralised with TRIS, pH 8.8. Purified BRD-6929 antibodies had been concentrated using a Centricon Plus\20 centrifugal filtration system (Millipore, Billerica, Massachusetts, USA), and immunoglobulin (Ig)G was assessed by nephelometry. Evaluation of PKC in NSCLC RNA was isolated from five NSCLCs of sufferers without PCD using the RNeasy Mini Package (Qiagen, Santa Clarita, California, USA), and treated with DNA\free of charge DNAse (Ambion, Austin, Tx, USA). After retrotranscription with SuperScript II invert transcriptase (Invitrogen, Carlsbad, BRD-6929 California, USA), the BRD-6929 cDNA samples were normalised by glyceraldehyde\3\phosphate dehydrogenase expression and amplified with PKC\specific primers then. Frozen (n?=?5) or paraffin\wax\inserted (n?=?6) NSCLC areas from sufferers without PCD, and paraffin\polish\embedded sections in the NSCLC of the individual with PCD with antibodies to PKC were probed with a particular polyclonal antibody to PKC (Santa Cruz Biotechnology, Santa Cruz, California, USA) utilizing a regular avidinCbiotin immunoperoxidase technique. Outcomes We identified 9 sufferers with classical NSCLC and PCD. Desk 1?1 summarises the primary clinical features. Six sufferers were men as well as the median age group at medical diagnosis BRD-6929 of PCD was 63 (range 47C73)?years. Two sufferers had recovered from PCD during tumour completely.
Outcomes demonstrated that OBPs play a particular function in olfactory conception, suggesting there’s a direct relationship between the appearance degree of OBPs and the power of pests to perceive smells
Outcomes demonstrated that OBPs play a particular function in olfactory conception, suggesting there’s a direct relationship between the appearance degree of OBPs and the power of pests to perceive smells. sensilla (arrow). (D) Information on little placoid sensilla (SP), and type I (CI) and type II (CII) coeloconic sensilla in the 6th portion encircled by cuticular fringes (arrowheads). (E) Details of porous framework on the top of huge placoid sensillum (arrowheads). (F) Information on placoid sensillum of 5th portion and type I trichoid CCNE2 sensilla (arrow) with grooved surface area (arrow in (F)). Porous buildings had been visible over the flat surface over the placoid sensillum of the portion Glesatinib hydrochloride (arrowhead). (G,H) Placoid sensilla (supplementary rhinaria) on another portion (white arrowhead in (G,H)) and trichoid sensilla type I (arrow in (G,H)). Pubs in (A,C,F), 10 m; pubs in (A,B,F), 500 nm; club in (B), 2 m; pubs in (D,E), 2 m; club in (G), 100 m; club in (H), 20 m. Picture_4.TIF (1.1M) GUID:?8BF33D63-5FF2-45ED-A19C-3A5FBD446716 FIGURE S5: Alignment of amino acid sequences of candidate OBPs from and constant expression level in areas of the body. Picture_6.PDF (305K) GUID:?096969C3-69EF-4F13-BFEC-CC7FEB759C04 FIGURE S7: Comparative expression degree of OBPs in various areas of the body (A,B) and in various nymphal instars (C,D) calibrated on and 0.05, ?? 0.01, ??? 0.001)). (A,B) Lg, hip and legs; Compact disc, cornicles-cauda; Hd, mind; Bd, body; An, antennae. Calibrator test: antennae. (C,D) I, 1st nymphal instar; II, 2nd nymphal instar; III, 3rd nymphal instar; IV, 4th nymphal instar; Ap, apterous adults; Al, alata adults. Calibrator test: 1st nymphal instar. Picture_7.PDF (566K) GUID:?F6F367CF-D839-404C-A3F2-2FB3F6561A3E FIGURE S8: Whole-mount immunolocalization experiments teaching the lack of sign for in antenna (A), and in the mouthparts (C,F), in the cauda (D,G,We), and in cornicles (E,H,J). (B) Detrimental control where the principal antibodies had been omitted. Pubs in (A,B), 30 m; pubs in Glesatinib hydrochloride (C,F), 10 m; pubs in (D,G,I), 50 m; pubs in (E,H,J), 20 m. Picture_8.TIF (397K) GUID:?083506A1-D067-46DD-AA89-862109CBAF4C Data Availability Glesatinib hydrochloride StatementThe fresh data accommodating the conclusions of the manuscript will be made obtainable with the authors, without undue reservation, to any kind of experienced researcher. Abstract Chemoreception in pests is normally mediated by many elements interacting at different amounts and including odorant-binding proteins (OBPs). Although latest studies demonstrate which the function of OBPs can’t be limited to an solely olfactory role, which OBPs have already been within organs generally not Glesatinib hydrochloride really linked to chemoreception also, their feature of binding substances remains undisputed. Learning the vetch aphid (Buckton), we utilized a transcriptomic method of recognize ten OBPs in the antennae and we analyzed the ultrastructural morphology of sensilla and their distribution over the antennae, hip and legs, mouthparts and cauda of wingless and winged adults by scanning electron microscopy (SEM). Three types of sensilla, trichoid, coeloconic and placoid, localized and distributed on antennae in different ways, mouthparts, cauda and legs, had been described. The appearance analysis from the ten OBPs was performed by RT-qPCR in the antennae and various other body parts from the wingless adults with different developmental levels and morphs. Five from the ten OBPs (had been also immunolocalized in the sensilla over the antennae, recommending a possible participation of these protein in chemoreception. had been highly portrayed in the minds and three of these (had been highly portrayed in the cornicles-cauda and two of these (had been immunolocalized in cornicles and in cauda, recommending a possible brand-new function not linked to chemoreception. Furthermore, the response of to different the different parts of the security alarm pheromone was evaluated by behavioral assays on wingless adult morph; (-)–pinene and (+)-limonene had been discovered to end up being the components generally eliciting an security alarm response. Taken jointly, our results signify a street map for following in-depth analyses from the OBPs involved with several physiological features in suggest that OBPs play an integral function in chemoreception. RNAi was utilized to lessen the appearance of OBPs in and (Biessmann et al., 2010; Pelletier et al., 2010), in (Swarup et al., 2011) and in (Zhang et al., 2017). Outcomes showed that OBPs play a particular function in olfactory conception, recommending there’s a immediate relationship between the appearance degree of OBPs and the power of pests to perceive smells. Previous studies discovered that OBP76a (LUSH) mutants, performed an essential function in binding and mediating the identification from the sex pheromone 11-tests (Kruse et al., 2003). It’s been showed that deleting OBP28a in basiconic sensilla didn’t reduce the pests capability to react to olfactory stimuli (Larter et al., 2016), recommending that OBP28a is not needed for odorant transportation. Co-workers and Larter hypothesize a book function for OBP,.
2011;121:1768C81. activation of Hedgehog/Gli2 signaling in Lgr5-expressing stem cells is enough to operate a vehicle gastric adenocarcinoma advancement in mice, recognize a critical requirement of mTOR signaling in the pathogenesis of the tumors, and underscore the need for tissue framework in determining stem cell responsiveness to oncogenic stimuli. ; 2) a transgene having a Cre-inducible change tetracycline transactivator (rtTA) inserted in to the broadly-expressed ROSA locus (mice, abbreviated allele and doxycycline-regulated tet transactivator allele, to attain restricted, conditional GLI2A appearance in adult mice. B. General system for tamoxifen (TAM) dosing and doxy treatment. C. Stomach regions and compartments, with blue text message indicating where in fact the Lgr5 promoter is normally active. Crimson dashed series along better curvature signifies where tummy was trim to expose mucosa (D) and prepare tissues for WRG-28 sectioning. D. Tummy gathered after 3 weeks of GLI2A induction included huge polypoid tumors in antrum that histologically resemble individual gastric adenocarcinomas. Vertical lines in correct panels illustrate proclaimed thickening of tumor in accordance with WRG-28 control antral mucosa, as well as the asterisk signifies ulceration. E. Invasion of tumor cells in to the submucosa with development of atypical gland-like buildings. F. Histologic credit scoring showing speedy neoplastic development in mice, with 88% of mice exhibiting either early or advanced gastric cancers at 3 weeks. G. Early tumor advancement (dashed series) close to the squamocolumnar junction. H. Full-blown gastric tumors demonstrated histological heterogeneity with two distinctive epithelial morphologies: extremely disorganized, atypical-appearing cells that exhibit GLI2A, with neighboring GLI2A-negative hyperplastic antral glands (asterisk). I. RNA hybridization discovered canonical Hh focus on genes (& mice (= 37) developing grossly noticeable tumors after 3 weeks of doxycycline treatment. H&E staining uncovered large tumor public with morphologic features comparable to those observed in individual gastric adenocarcinoma, including lack of differentiated cell types, tumor nodules filled with multiple levels of disorganized epithelial cells, cytologic atypia, and abundant tumor stroma using a blended inflammatory infiltrate (Amount 1D, 1E, Supplementary Amount 1, and below). Some tumors had been ulcerated (Amount ?(Figure1D);1D); furthermore, tumor cells WRG-28 occasionally invaded the submucosa and muscularis propria (Amount ?(Amount1E,1E, Supplementary Amount 1). Both these findings have emerged in advanced gastric cancers in human beings also. We analyzed tissues areas from a cohort of mice (= 41) euthanized at many time-points (Amount ?(Figure1F)1F) to get additional insight in to the procedure for neoplastic development, with representative types of histologic scoring shown in Supplementary Figure 1. Seven days after transgene induction, 86% of mice included parts of low-grade dysplasia; by fourteen days, CD36 43% of mice acquired either low-grade or high-grade dysplasia, with the rest of the 57% of mice have scored as early gastric cancers; by three weeks, 65% of mice had been have scored as having early gastric cancers and 23% as advanced gastric cancers, with dysplasia observed in the rest of the 12% (Amount ?(Figure1F).1F). Although grossly noticeable tumors in stomachs of mice had been limited by the gastric antrum, the region near the initial gastric gland from the corpus on the squamocolumnar junction (Amount ?(Amount1C)1C) also frequently included disorganized, dysplastic-appearing cells (Amount ?(Amount1G),1G), reflecting the appearance pattern from the drivers in adult mice . Individual gastric adenocarcinomas display intratumor heterogeneity [19 often, WRG-28 20], that was detected in mice also. Full-blown tumors included epithelial cells with two distinct morphologies: disorganized cells often exhibiting cytologic atypia and a higher nuclear to cytoplasmic proportion; and neighboring hyperplastic gastric glands made up of cells with abundant eosinophilic cytoplasm, an eccentric nucleus, and little if any atypia (Amount ?(Amount1H).1H). Appearance from the GLI2A transgene, discovered by immunostaining for the MYC.
It really is crystal clear that for a far more complete picture therefore, the coupling of water and protein dynamics must be considered
It really is crystal clear that for a far more complete picture therefore, the coupling of water and protein dynamics must be considered. 3.4. structures found in MD simulation; B. Description and residue amounts of structural waters; C. Observed situations of changed (red) and held (blue) drinking water substances in crystal buildings from the bromodomains; D. Health spa results for drinking water binding; E The percentage from the transitions of drinking water 1C5; F. PDB rules of crystal buildings found in the study of drinking water network.(DOCX) pone.0186570.s002.docx (36K) GUID:?20618A34-E58E-41BB-8139-445D96B5F445 S3 Document: Zipfile with parameters and analysis scripts. These scripts may also be bought at https://github.com/XiaoxiaoZhangOuc/PLOS1_BRD.(ZIP) pone.0186570.s003.zip (40K) GUID:?A57D8E69-469F-4504-8C33-CBDD460438BE Cucurbitacin E S4 Document: Crystal structure survey. (XLSX) pone.0186570.s004.xlsx (146K) GUID:?35DC2782-7A5B-4B3B-8B5A-12DE35E24148 Data Availability StatementAll relevant data are either contained in the Helping Information or accessible via the pdb (www.rcsb.org/pdb/), in the helping details of cited personal references or on github. Please see S1 S2 and Document Apply for particular accession quantities Cucurbitacin E and URLs. Abstract Bromodomains are epigenetic visitors of acetylated lysines that are essential elements of histone tails. The 61 bromodomains in human beings are extremely conserved but particularly bind to broadly differing identification motifs structurally, recommending that dynamic than static elements are in charge of recognition selectivity rather. To check this hypothesis, the dynamics from the binding sites and Cucurbitacin E structural drinking water substances of four bromodomains (ATAD2, BAZ2B, BRD2(1) and CREBBP) representing four different subtypes is normally examined with 1 s MD simulations using the RSFF2 drive field. The various dynamics from the ZA-loops and BC-loops between your Cucurbitacin E four bromodomains network marketing leads to distinctive patterns for the starting and closing from the binding pocket. Therefore determines the full of energy and structural properties from the structural waters in the binding pocket, suggesting these waters aren’t only very important to the identification itself, as continues to be proposed previously, but donate to the selectivity of different bromodomains also. 1. Launch Lysine acetylation continues to be found to try out a fundamental function during epigenetic legislation of gene appearance[1C6]. Bromodomains (BRD) are protein connections modules that selectively bind -N-lysine acetylation (KAc) and various other acylation motifs. 61 exclusive bromodomains, clustered into eight households based on series similarity, have already been reported to become contained in 46 chromatin regulator proteins in human beings. Phylogenetic analysis from the individual bromodomains recognizes eight subclasses, which subclass II, the bromodomain extra terminal protein (Wagers) family will be the greatest studied[8C11]. Because the initial three-dimensional framework of bromodomain was resolved by NMR spectra in 1999, crystal and/or alternative buildings of over 40 bromodomains with and without destined substrates or inhibitors have already been released and released. All bromodomains talk about a conserved tertiary framework fold comprising a left-handed four-helix pack; using the hydrophobic acetyl-lysine binding site at one end from the helix pack formed with the ZA loop and BC loop (Fig 1A). Open up in another screen Fig 1 (A) Overlay of apo buildings of 4 bromodomains: teal: ATAD2, (3DAI, 1.95?); sterling silver: BAZ2B (3G0L, 2.03?); red: BRD2(1) (1X0J, 1.80?); precious metal: CREBBP (3DWY, 1.98?) (B-F) close-up of binding sites with structural waters proven. (F) Sequence position from the four Rabbit Polyclonal to APLP2 (phospho-Tyr755) bromodomain pocket in the WPF shelf to best of helix C. The greyish colored regions display series distinctions that may impact protein dynamics, as well as the crimson frames display the conserved motifs (WPF, PVD, PDY, PMD, V1, N1, YN) through the whole bromodomain family, that are utilized as markers to define water network. The bromodomains proven in Fig 1AC1E not merely represent the normal buildings of four from the eight phylogenetic subclasses, but may also be typical illustrations for the wide range of biological processes bromodomains are involved in. For example, the C-terminal domain name of BRD2 was found to be important for chromatin conversation and regulation of transcription and alternative splicing. I-CBP112, an inhibitor of the subclass III CREBBP, has been found to significantly reduce the leukemia-initiating potential of MLL-AF9(+) acute myeloid leukemia blasts both in vitro and in vivo. Studies show that this subclass IV ATAD2 is usually a generalist facilitator of chromatin dynamics in embryonic stem cells, and that bromodomain mutations can disable ATAD2s ability of promoting cancer cell proliferation. The function of the subclass V bromodomain BAZ2B.
Oddly enough, in vitro, both PFA and a siRNA against SLC20A1 avoided Pi-mediated down-regulation of Akt-1 mRNA transcript amounts, indicating that transporter is mixed up in regulation from the PI3K/Akt pathway
Oddly enough, in vitro, both PFA and a siRNA against SLC20A1 avoided Pi-mediated down-regulation of Akt-1 mRNA transcript amounts, indicating that transporter is mixed up in regulation from the PI3K/Akt pathway. Overexpression of Akt-1 (pCMVAkt-1) avoided both Pi-induced apoptosis and mineralization of VIC lifestyle. These results strongly claim that overexpression of SLC20A1 promotes apoptosis and mineralization by altering the known degree of Akt-1. Launch Calcific aortic valve disease (CAVD) is normally a common disorder from the maturing population . Regardless of the high prevalence of the condition, there is indeed far no treatment for CAVD. To the effect, randomized studies with statins possess indicated a lipid-lowering technique in sufferers with CAVD is normally no more effective compared to the placebo , , . Different risk elements, such as for example age, man gender, diabetes, and hypertension, have already been discovered in CAVD . Mineralization from the aortic valve may be the main culprit in the introduction of CAVD. The main element molecular processes resulting in the mineralization from the aortic valve are simply beginning to end up being understood, which understanding is very important in devising book medical therapies. Regional fat burning capacity and phosphate managing (Pi) are a significant pathway in the control of pathological tissues mineralization . Research suggest that Pi transporters, such as for example SLC20A1/Pit1 play a significant function in the mineralization of arteries . To the impact, intracellular channelling of Pi may promote mineralization. Nevertheless, the Dalbavancin HCl mobile pathways that are prompted following intracellular entrance of Pi stay largely unexplored. It really is worthy of noting that phosphate-generating enzymes are extremely portrayed during mineralization and elevate the focus of Pi in the extracellular space. One latest study indicates which the ectonucleotidase enzyme, ectonucleotide pyrophosphatase/phosphodiesterase-1 (ENPP-1), is normally highly portrayed in CAVD which it plays a part in the elevation of extracellular Pi amounts in valve interstitial cells (VICs), which will be the primary cellular element of the aortic valve . It comes after that Pi may be channelled in to the intracellular space, adding to the mineralization from the aortic valve. Though it is well known that Pi induces mineralization in vascular even muscles cells and VICs by marketing apoptosis, the function of Pi transporters in this technique, aswell as the string of events resulting in programmed cell loss of life, hasn’t however been elucidated  completely. In this scholarly study, we hypothesized that Pi transporters play a significant role in providing indicators of apoptosis in VICs by changing the amount of Akt, a kinase involved Dalbavancin HCl with cell survival. Strategies Patients We analyzed 50 aortic valves which were explanted from sufferers during aortic valve alternative to CAVD. Control non-calcified aortic valves (n?=?28) with regular echocardiographic analyses were attained during center transplant procedures. Sufferers using a previous background of rheumatic disease, endocarditis, and inflammatory illnesses had been excluded. Valves with an aortic valve regurgitation quality >2+ had been excluded. Patients with COL12A1 minimal still left ventricular ejection small percentage (LVEF) (<40%) had been excluded. All sufferers underwent a thorough Doppler echocardiographic evaluation preoperatively. Doppler echocardiographic measurements included the still left ventricular stroke quantity and transvalvular gradients using the improved Bernoulli formula. The process was accepted by the Dalbavancin HCl neighborhood moral committee and up to date consent was extracted from the topics. Immunohistochemical Analyses Immunohistologic evaluation for SLC20A1 was Dalbavancin HCl performed using the rabbit antibody anti-SLC20A1 (Novus Biologicals, Oakville, ON, Canada). Slides had been after that incubated with HRP-conjugated and AEC substrate (Dako, Mississauga, ON, Canada). Tissues sections had been counterstained with hematoxylin. Rabbit serum was utilized as a poor control in immunohistologic tests. Valve Interstitial Cells Analyses and Isolation of Calcification Individual VICs were isolated by collagenase digestive function. To market calcification, cells had been incubated for seven days using a pro-calcifying moderate filled with: DMEM +5% FBS, 10?7 M insulin, 50 g/ml ascorbic acidity and NaH2PO4 at 2 mM. In a few experiments, phosphonoformic acidity (PFA) (1 mM) or LY294002 (50 M) (Sigma, Oakville, ON, Canada) was added as given. The calcium mineral content was dependant on the Arsenazo III technique (Synermed, Monterey Recreation area, CA, USA), which depends on the specific result of Arsenazo III with calcium mineral to make a blue.
MLT reduced 62% of total H2O2 creation, however when coincubated with DHA, H2O2 era increased in comparison to control, simply because shown in Body 3(a) (control: 0
MLT reduced 62% of total H2O2 creation, however when coincubated with DHA, H2O2 era increased in comparison to control, simply because shown in Body 3(a) (control: 0.098??0.007, MLT: 0.037??0.008, and DM: 0.144??0.009?pmol/min/106 cells). Beta Carotene Open in another window Figure 3 ROS perseverance. on coincubation. DHA isolated didn’t alter the oxidative phosphorylation price (OXPHOS), but reduced (< 0.001) the mitochondrial bioenergetic reserve capability (MBRC) which is closely linked to cell responsiveness to tension conditions. MLT, of DHA regardless, ameliorated OXPHOS and retrieved MBRC after coincubation. All incubations reduced AKT phosphorylation; nevertheless, only MLT by itself inhibited p-mTOR. MLT elevated p-ERK1/2 and, when mixed to DHA, elevated GSTP1 appearance (< 0.01). DHA didn't transformation the testosterone amounts in the moderate, whereas Beta Carotene MLT by itself or coincubated reduced by about 20%; nevertheless, any incubation affected AR appearance. Furthermore, incubation with luzindole uncovered that MLT results were MTR1/2-indie. In conclusion, DHA increased ROS creation and impaired mitochondrial function that was linked to AKT inactivation probably; MLT improved OXPHOS and reduced ROS that was linked to AKT/mTOR dephosphorylation, so when coincubated, the antiproliferative action was linked to mitochondrial bioenergetic modulation associated to ERK1/2 and AKT regulation. Together, these findings indicate the application of MLT and DHA towards preventing proliferative prostate diseases. 1. Launch Despite its multifactorial etiology, development and aggressiveness of prostate cancers (PCa) have already been linked to oxidative tension [1, 2] as well as the elevated creation of reactive air species (ROS) is certainly closely linked to modifications in the mitochondria . Such organelles play an essential role in every levels of malign change  and also have been linked to PCa because of decrease in apoptotic potential , pathogenic mutations in genes encoding the electron transportation string (ETC) respiratory complexes, and lack of mitochondrial integrity and DNA . Therefore, modulation of mitochondria physiology may be an excellent healing focus on, either in preventing tumor advancement or in the induction of cancers cell loss of life. Melatonin (MLT) is certainly a pleiotropic hormone with antioxidant properties that regulate mitochondrial activity [6C10] and continues to be investigated being a PCa suppressor . Sufferers with PCa display low MLT serum amounts in comparison with healthy individuals, using a significant decrease when harmless prostatic hyperplasia (BPH) advances to adenocarcinoma [11, 12]. Most situations of PCa (75%) are diagnosed in guys over 65 years , coincidental to the time when MLT synthesis is certainly decreased mitochondrial and  dysfunction boosts because of ROS creation [14, 15]. Relating to this proof, MLT supplementation in sufferers within risk age group of PCa (30C40 years of age) could be a fascinating chemoprevention technique . From its anticancer properties Aside, MLT continues to be looked into in conjunction with various other substances also, because of its capability to sensitize cells and potentialize the antiproliferative aftereffect of these substances by inhibition of success pathways, e.g., AKT . Within this framework, polyunsaturated essential fatty acids omega-3 (PUFA = 3) in the same gel, and one protein per gel furthermore to check or one-way ANOVA accompanied by Tukey check (post hoc); non-parametric distributions to Mann-Whitney or Kruskal-Wallis check accompanied by Dunn check (post hoc). < 0.05 was considered different statistically. 3. Outcomes 3.1. Pro- or Antimitogenic Activities of DHA in PNT1A Cells Are Period- and Concentration-Dependent All DHA concentrations examined within 24?h, except 10?< 0.05 was motivated as different statistically. All proliferation assays had been performed in triplicate, and three indie events regarded for statistical evaluation. Beliefs present the mean of SEM and absorbance. 3.2. MLT Reduced PNT1A Cell Proliferation MLT at physiological concentrations (1?pM and 1?nM) had zero influence on cell proliferation (Body 1(c)) but decreased in 1?< 0.05 was considered statistically different. At least 500 cells per treatment from three consecutive passages had been analyzed. Values present the mean of fluorescent systems per cell and SEM. 3.5. DHA Elevated Superoxide Anion Creation and MLT Bglap Alleviated ROS Era DHA didn’t alter H2O2 creation by PNT1A cells in comparison with control (Body 3(a)). MLT decreased 62% of total H2O2 creation, however when coincubated with DHA, H2O2 era elevated in comparison to control, as proven in Body 3(a) (control: 0.098??0.007, MLT: 0.037??0.008, and DM: 0.144??0.009?pmol/min/106 cells). Open up in another window Body 3 ROS perseverance. Creation of (a) hydrogen peroxide (H2O2)beliefs present pmol of H2O2/min/106 cells and SEM; creation of (b) superoxide anion (O2?)beliefs present the fluorescence strength (FI) per cell??104. Star of sections (a) and (b): C: control (automobile incubation); DHA: incubation with 100?< 0.05 was considered statistically different. Three indie tests in triplicate had been regarded for statistical evaluation of H2O2 creation with least 250 cells for O2?. (c) Recognition of O2? creation after MitoSOX incubation. Pictures captured with 40x goal with an inverted fluorescence microscope. DHA doubled whereas MLT by itself decreased 40% of superoxide anion creation (Statistics 3(b) and 3(c)) by PNT1A Beta Carotene cells in comparison with control. Coincubation attenuated.
Supplementary MaterialsFigure S1: Effect of vorinostat in Ba/F3 T315I cells. imatinib. Lately, second-generation ABL TKIs dasatinib (Sprycel?) and nilotinib (Tasigna?) have already been employed for sufferers resistant to or intolerant of imatinib therapy more and more, and also have been accepted for front series use in sufferers Rabbit Polyclonal to SF3B3 with chronic stage CML . Nevertheless, one stage mutation, T315I, situated in the gatekeeper Rovazolac area from the ATP-binding site, confers level of resistance to imatinib, dasatinib, and nilotinib . As yet, no viable treatment plans had been available for sufferers in whom ABL TKIs fail due to the current presence of T315I mutation. Hence, alternative strategies must improve the final result of CML sufferers having the T315I mutation. Ponatinib, known as AP24534 also, is an dental, multi-targeted TKI. Ponatinib works well at nanomolar amounts against T315I and various other stage mutations , . This TKI continues to be investigated within a pivotal stage 2 scientific trial in sufferers with resistant or intolerant CML and Ph-positive severe lymphoblastic leukemia . Histone acetyltransferases and histone deacetylases (HDACs) function antagonistically to regulate histone acetylation . HDACs control chromatin remodeling and are important in the epigenetic rules of various genes. Irregular activity or manifestation of HDACs has been found in a broad range of tumor types . An HDAC inhibitor (HDACi) blocks the activity of specific HDACs. Preclinical data suggest a role for HDACi like a potential fresh treatment in several tumor types, including hematological malignancies . In this study, we investigated ponatinib activity against Ph-positive leukemia cells transporting the T315I mutation. We also examined the effectiveness of HDACi vorinostat in combination with ponatinib in various cell lines. This study also targeted to explore the molecular mechanism of ponatinib resistance by using BCR-ABL-expressing cell lines with point mutations. Furthermore, co-treatment with vorinostat and ponatinib suppressed growth in Rovazolac ABL TKI ponatinib-resistant clones. Strategies and Components Reagents and antibodies Ponatinib was purchased from Shanghai Biochempartner Co., Ltd. (Shanghai, China). The HDAC inhibitor vorinostat (suberoylanilide hydroxamic acidity) was supplied by Merck & Co (NJ, NJ). Share solutions of vorinostat and ponatinib had been dissolved in dimethyl sulfoxide (DMSO) and eventually diluted to the required focus in the development moderate. Anti-phospho Abl, anti-phospho Crk-L, anti-cleaved caspase 3, anti-poly (ADP-ribose) polymerase (PARP), and anti-acetyl-histone H4 antibodies had been bought from Cell Signaling (Beverly, MA). -Tubulin and -actin antibodies had been supplied by Santa Cruz Biotechnology (Dallas, TX). Various other reagents had been extracted from Sigma (St Louis, MI). Cell lifestyle and mutagenesis The individual CML cell series K562 was extracted from American Type Lifestyle Collection (ATCC; Manassas, VA). The BCR-ABL-positive cell series Ba/F3 BCR-ABL with wild-type and mutant Ba/F3 cells (T315I) once was set up . These cells had been preserved in RPMI1640 moderate supplemented with 10% heat-inactivated fetal bovine serum filled with 1% penicillin/streptomycin within a humidified incubator at 37C. Ponatinib-resistant Ba/F3 cells were set up  previously. BCR-ABL mutation evaluation Genomic DNA was isolated using the DNeasy package (Qiagen, Valencia, CA). Particular subregions of cDNA had been amplified by high-fidelity PCR from genomic DNA with a Stratagene Autocycler (Robocycler Gradient 40). The primers employed for the reactions had been SH3-SH2-upper, check, accounting for Rovazolac unequal variance. P beliefs 0.05 were considered significant. In a few tests, data for evaluation of multiple groupings are provided as mean S.D. and examined with two-way ANOVA. Outcomes Ponatinib inhibits Rovazolac development and induces apoptosis in K562 and T315I mutant cells We examined the efficiency of ponatinib within a representative BCR-ABL-expressing cell series, specifically, K562. K562 cells had been treated with differing concentrations of ponatinib for 72 h. Treatment with ponatinib for 72 h considerably reduced development of K562 cells at nanomolar amounts (Amount 1A). We examined the intracellular signaling of ponatinib also. Immunoblots demonstrated that phosphorylation of both BCR-ABL and its own downstream molecule Crk-L was considerably decreased after treatment with ponatinib. Furthermore, caspase 3 and PARP activity was elevated by ponatinib within a dose-dependent way (Amount 1B). It’s been reported that ponatinib provides powerful activity against T315I mutant cells . Hence, we analyzed ponatinib activity through the use of Ba/F3 BCR-ABL T315I mutant cells. In concordance with reported outcomes, ponatinib inhibited development in Ba/F3 T315I cells at low concentrations (Amount 1C). Immunoblot evaluation revealed that inhibition was due to.
Supplementary MaterialsDocument S1. the partnership of bone tissue marrow to transplanted cells continues to be unknown. Here, we quantified failing of hPSC-HPCs to survive 24 also?hr post transplantation. Across many hPSC-HPC differentiation methodologies, we identified having less CXCR4 function and expression. Ectopic CXCR4 conferred CXCL12 ligand-dependent signaling of hPSC-HPCs in biochemical assays and elevated migration/chemotaxis, hematopoietic progenitor capability, and proliferation and success following transplantation. This was along with a transcriptional change of hPSC-HPCs toward somatic/adult resources, but this process didn’t make long-term HSC xenograft reconstitution. Our outcomes reveal that systems involving CXCR4 ought to be geared to generate putative HSCs with function from hPSCs. occurring within the initial 24?hr, in spite of solid hematopoietic progenitor capability detected for weeks HSCs from hPSCs. Outcomes Faulty Retention of hPSC-HPCs Early Garcinol properties of hPSC-HPC integration in to the BM haven’t been explored by immediate hand and hand comparisons with individual adult/somatic HPC resources. Cord bloodstream (CB) is designed for experimentation being a somatic way to obtain HSCs that create long-term multilineage hematopoietic engraftment in xenograft versions (Boyd et?al., 2017). Furthermore, transplantation of CB cells continues to be used medically for long-term reconstitution of donor-derived healthful hematopoiesis in sufferers Garcinol (Cutler et?al., 2013). Therefore, we utilized CB being a way to obtain transplantable cells to investigate early HPC behavior and evaluate this straight with HPCs produced from hPSCs. hPSC-derived HPCs had been produced using embryoid body (EB) development and differentiated with hematopoietic cytokines and BMP4 (Chadwick et?al., 2003), and were applied to EB day 15 for transplantation and analysis. Somatic and hPSC-HPCs usually do not talk about comparable frequencies of phenotypic or useful progenitors, as quantified by individual specific Compact disc34+Compact disc45+ cell surface area appearance (versus mouse?mCD45; Body?1A) and colony forming device (CFU) structure (Body?S1A), respectively. These email address details are consistent with prior reports across a wide selection of methodologies to create phenotypic or useful progenitors from hPSCs (Doulatov et?al., 2013, Lee et?al., 2017, Ramos-Mejia et?al., 2014, Risue?o et?al., 2012, Saxena et?al., 2016, Tian et?al., 2006, Vodyanik et?al., 2006), in addition to nonhuman primate amounts represent transplanted mice, pooled from three performed tests with six harvest analyses independently. (E) Phenotype of CB and hPSC-derived HPCs from gathered BM. (F) Total mCD45ChCD45+Compact disc34+ cells maintained within the BM of injected (IF) and contralateral (CF) femurs. To assess BM retention from proliferation individually, just 24 and 48?hr data for CB shown. Data factors stand for transplanted mice, ? is certainly zero. Two-way ANOVA, ????p? ?0.0001. Data are symbolized as means SEM. (G and H) Total mCD45ChCD45+Compact disc34+ cells per injected femur. Same hPSC-HPC data in both panels. (I) CFU Garcinol from CB-transplanted BM, harvested at day 5. Arrowheads: reddish, burst-forming unit-erythroid; gray, CFU-granulocyte and/or -monocyte. (J) Total human CFU per harvested IF and CF BM. To assess BM retention of progenitors separately from cellular proliferation and growth, only 24 and 48?hr retention data for CB shown; day 3 and 5 data omitted. Data points symbolize transplanted mice, ? is usually zero. One-way ANOVA, ??p? 0.01. Data are represented as means SEM. (K and L) Total human CFU per IF. Same hPSC-HPC data in both panels. (M) Linear regression of total CB phenotypic versus functional HPCs quantified per IF. Data points symbolize transplanted mice. Using this cautiously quantitated approach to phenotypically and functionally enumerate equivalency of transplanted cells, human CB versus hPSC-derived HPCs were injected into the femurs of murine recipients, where the BM was assessed for human chimerism at the functional and phenotypic level at multiple time points within the first week. At the same time points as injected femur assessment, we decided migration capacity by analysis of contralateral femur BM, spleen, and lungs (Physique?1C). The number of individual mice from four transplant groups were compared at 24?hr and 2, 3, and 5?days as indicated Garcinol Garcinol (Physique?1D) to address the classical time of homing, within 24?hr (Jetmore et?al., 2002), while being inclusive of much longer intervals of homing also, as much as 4?times (Foster et?al., 2015). The regularity of individual hematopoietic cell chimerism was uncommon, but could possibly be captured by stream cytometric evaluation for individual HPCs (mCD45ChCD45+Compact disc34+, Statistics 1E, S1B, and S1C). Phenotypic CB HPC enlargement was evident inside the injected femur BM well in this timeframe (Amount?1E). As predicated (Wang et?al., 2005), intra-femoral shot supplied an Cryab engraftment benefit to retain HPCs within the injected femur, even though.
Supplementary Materialsaging-12-102733-s001. RhoA, Cdc42, Acetylated -tubulin and MMP2. All the data acquired support the concept that autocrine CXCL8 signalling takes on a key part in the activation of an aggressive phenotype in main glioblastoma cells and U-87MG cell collection. These results provide fresh insights about the potential of a pharmacological approach focusing on CXCR1/CXCR2 pathways to decrease migration and invasion of GB cells in the brain parenchyma, one of the principal mechanisms of recurrence. data display high CXCR1/ CXCR2 overall levels (in permeabilized cells) as compared to low CXCR1/CXCR2 surface levels (in not permeabilized cells), because of the peculiar membrane turnover and cellular trafficking. This evidence is consistent with the high CXCL8 levels recognized in the medium and good hypothesis that an autocrine CXCL8-induced signalling, including both CXCR1 and CXCR2, is triggered in GB. Open in a Ruboxistaurin (LY333531 HCl) separate windows Number 1 The GB cellular models present different degrees of CXCR1/2 and CXCL8. ELISA assay was utilized to quantify the quantity of CXCL8 secreted in the supernatant mass media from GB principal cell lifestyle and U-87MG cells (A). Data are means SEM of three different natural replicates (n=3). (B) Consultant cytofluorimetric evaluation for CXCR1 and CXCR2 proteins amounts in GB principal cell lifestyle and U-87MG cell series. Cytofluorimetric profile pictures are representative one. Cytofluorimetric evaluation had been performed in permeabilized or not really permeabilized Ruboxistaurin (LY333531 HCl) cells. tCXCR1/2: total proteins amounts in permeabilized mobile samples; sCXCR1/2: surface area protein amounts in not really permeabilized cellular examples. CXCR1/CXCR2 allosteric inhibition elicits suppression from the invasiveness and migration without cytotoxic impact in GB cells In the next set of tests, the dose-dependent aftereffect of DF2755A, a selective and powerful dual CXCR1/CXCR2 non competitive allosteric inhibitor , was assayed in 0.1-5 M focus range on cell viability (Supplementary Figure 2). No noticeable cytotoxic effects had been noticed at any focus used; upon this basis, the 0.1 M focus every day and night was particular as the experimental condition for the next tests. In Statistics 2 and ?and3,3, the full total benefits of CXCL8-induced cell chemotaxis and zymography assays are reported for both cellular types. DF2755A treatment reduced the Normalized Cell Index CC2D1B (NCI) linked to cell chemotaxis (Statistics 2A and ?and3A),3A), and significantly reduced the migration slope (about 45% in GB primary cell civilizations and 60% in U-87MG cells) in comparison to neglected cells. The slope methods how NCI adjustments as time passes and can be used to look for the prices of chemotaxis occasions. In Statistics 2B and ?and3B3B the MMP2 activity, analysed by gelatin zymography assay, is reported. CXCL8 signalling inhibition by DF2755A administration induced, in both mobile models, the reduced amount of MMP2 activity portrayed as energetic MMP2/latent MMP2 proportion. Ruboxistaurin (LY333531 HCl) A significant reduction in the proportion was seen in DF2755A treated cells in comparison to neglected cells. In the same -panel live imaging wound evaluation of control and treated glioblastoma cells are proven. You’ll be able to discover that in the current presence of DF2755A cell migration resulting in wound closure was considerably delayed (Statistics 2C and ?and3C).3C). Wound width, assessed by Incucyte evaluation software and portrayed in m was low in untreated treated cells. Open in a separate window Number 2 Cell chemotaxis assay in GB main cell tradition under DF2755A treatment. (A) Normalized cell index after 24 hours of treatment, the cell migration was adopted for 12 hours. The supernatants of chemotaxis assay were collected to perform gelatin zymography. In (B) a representative gelatin zymography and relative densitometry analysis indicated as relative models of active MMP2/latent MMP2 percentage. (C) Representative images of wound closure at 0 hours (top) and 24 hours (bottom), the reddish lines represent the edges Ruboxistaurin (LY333531 HCl) of the starting scratch, while the green areas represent the wound closure. The wound analysis was displayed as wound width (m) after 24 hours of migration. Data are means SEM of three different biological replicates (n=3). Statistical analysis was performed from the unpaired Student’s t-test (with Welchs correction). *, p .

domain: biology


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RI and Connecticut weren’t part of our itinerary. We were at Boston and on the way back to New Jersey my uncle decided to take a detour. It’s roughly an hour or two away and it would be a waste to pass the opportunity to see these places. So far I’ve been to DC, Chicago and my home bases, NY and NJ. It would be nice to expand my state vocabulary.
If you’re wondering, these photos don’t necessarily describe Rhode Island. The houses and everyday living look absolutely different. Rhode Island is actually very nautical and colorful! Sadly I wasn’t able to take photos in the car. I was somehow exhausted from Boston. I felt pretty shameless for sleeping along the way to RI. If you have seen Wes Anderson’s Moonrise Kingdom, it briefly sums up the aesthetic of Rhode Island and Connecticut. If I were to go back to the US, I’d definitely go back and explore more.
The Breakers was the first thing that came across my aunt and uncle’s minds when we decided to pass by Rhode Island. Most of the images below are from the mansion.
I think these houses are from Boston. Just passed by it on the way to the next state.
At the Vanderbilt Mansion. We only had 3 hours to spare and they felt this was an essential site to go through.
Normally you’re not allowed to take photos inside but it’s the Christmas season so the staff allowed visitors to take photos of the trees—just the trees! If you’re caught taking photos of anything else, you’ll be reprimanded. I swear, those guys, they breathe down your necks!
This is just the backyard. Even the sea is part of the backyard! The mansion is insane. I’ve never seen anything like it in real life. You can check the website to see what I’m talking about.
Me + wind. It was really chilly that day and you pretty much don’t have a choice, just brave it up. Even if you wear something thick, your head and hands get all the beating.
At Mystic Seaport Connecticut. At sunset.
Afterwards we ate good food from a nearby fancy restaurant. Connecticut is lovely (at least on the coastal side, since this is the only part I’ve seen). There’s something really nostalgic about it. Or maybe this is what I’m feeling because I only see this in the movies.
When I was in NY last December my aunt and uncle invited me to go on a road trip to Boston so at least I could see it before I leave. My aunt and uncle spoil me too much. They take me almost everywhere!! I think some of my fondest memories in the US are brought about by my stay with them. The ride from Northern NJ to Boston took about 4 hours. Just as they say, the East Coast is rich in history. Old architecture is always a feast for the eyes. Tons of photos under the cut, mostly reddish. Boston is very red!
I was working on this and my sister hijacked my playlist and made me listen to G-Dragon all throughout. Basically this hip deer is fueled by Korean hiphop.
#wip for Crested Tees. More to come!
Wolf x girl.
I did a short wip video of this on my Instagram. I’ll try to post process videos regularly! http://instagram.com/valerieannchua
Doing commissions at the studio. I only have until the 31st here. :( Just need to finish 2 more paintings then I’m out. I love clients who say “do whatever you like!!!” So nice of them. ;_;
FInd me at the center and you will find a gaze that is most cordial

domain: geography


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2014 was a good year for the U.S. economy: the unemployment rate fell to 5.6%, a significant drop from the 6.7% where it started in January, according to the U.S. Bureau of Labor Statistics. This marks the first time it has been that low since June 2008. A similar story could be found in Connecticut, where the unemployment rate dropped from 7.4% at the end of last year to 6.5% at the end of this one. Hartford had a relatively solid year as well, and saw an improvement in its unemployment rate; after closing 2013 with 6.8% unemployment, it closed 2014 with 6.2%.
Source : Cushman & Wakefield

domain: news


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Tuesday 10 September 2013
7pm • Pelham House, Lewes
Tickets £50 from Pelham House on 01273 488 600
Pelham House hotel and Harveys Brewery, invites you to sample a selection of Harveys seasonal beers, alongside a menu of local and foraged food prepared by head chef Glenn Lester. Miles Jenner, head brewer at Harveys, will be on hand to talk through the beers and reveal all about the process of brewing.
Your ticket includes canapés and drinks on arrival, and a four course supper with matched beers. Over night accommodation is available for an additional £75 per room including full English breakfast.

domain: travel


New Jersey closed a loophole identified in an Asbury Park Press investigation that allowed cops to go on potentially deadly car chases for traffic infractions.
The Asbury Park Press revealed the secret internal affairs file of a killer cop, which showed his department knew about his “domestic violence incidents” before he fatally shot his ex-wife.
After we exposed how cops can strike deals to keep their discipline hidden from future employers, New Jersey passed a law that demolishes those agreements and requires departments to share an officer’s internal files when they’re seeking a new job.
I convinced two state lawmakers to attend a public forum on police accountability in 2018. They later sponsored legislation that fixed problems we exposed.
Following our reporting on New Jersey’s lack of a police licensing standard, the state’s police training commission recommended licensing for cops.
New Jersey policymakers consider licensing police officers and implementing a pre-academy physical test for police recruits, following reporting by the USA Today Network.
A lawmaker was inspired to file a police accountability bill following our reporting.
The New Jersey Attorney General strengthened police oversight after two of our stories. We exposed how charges by a cop found to have lied ruined one man’s life and sent two men to jail. We also informed a prosecutor’s office a cop they fought to fire was still on the job – and charging citizens they were taking to court.
Breaking the news that a town agreed to two huge whistleblower settlements prompted an upset victory in the race for mayor.
Working with confidential sources was crucial to breaking an essential local story: a police captain was drunk and on drugs at the time of his fatal car crash.
A follow story the next day broke more news about the officer’s past.
This haunting daily marks the conclusion of a child abuse case, be sure to check out the enterprise piece that reveals how the boy slipped through systemic gaps.
Rather than reporting a blizzard from the office, this story is told through the eyes of those on the front lines.


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Renal and urinary tract anomalies have been reported to occur with a number of chromosomal abberations and a variety of müllerian developmental anomalies. Historically, the XY female has been privileged to remain free from these associated urologic complications. Presented are two types of the XY female in whom a major renal anomaly was found. It is possible that a potentially more frequent association of developmental anomalies has been overlooked. The urinary systems of XY females, when identified, should be investigated.
© 1978 The American College of Obstetricians and Gynecologists

domain: medical


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Known for its historical sites and boating, Dubrovnik offers a lot for the traveller planning a getaway. With 205 beach hotels and other accommodation options to pick from in this seaside destination, it's a breeze to find one that meets your needs.
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The closest major airport is in Dubrovnik (DBV), located 9.6 mi (15.5 km) from the city centre. You can explore water travel options at the local port.


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Hi Psynews! I'm excited to present my debut album. The Maniac - A Treatise On Acid. What to expect you ask? Well, Acid of course! 10 tracks with blistering 303 and an acid drenched Goa Trance sound. Tracklist: 01) The Maniac - Praise The Acid 02) The Maniac - Computer Controlled 03) The Maniac- MK Ultra 04) The Maniac - Losing Sanity 05) The Maniac - Hypnogogic 06) The Maniac - Back To Psychosis 07) The Maniac - Acid In The Circuit 08) The Maniac - Corrosion 09) The Maniac - Overdose 10) The Maniac - A Treatise On Acid Band Camp: http://bit.ly/2Bz6b7X Psyshop: http://bit.ly/2X84rLM Spotify: https://spoti.fi/2SYVTro Amazon: https://amzn.to/2V1THgl GooglePlay: http://bit.ly/2tl9smI JunoDownload: http://bit.ly/2Grj08q OUT NOW on Timewarp Records. Timewarp Records proudly presents amazing Goa album journey from super talented Maniac Project called " A Treatise On Acid"!! The Maniac is a Goa Trance project from India by B Deepak created with the sole intention of making Extra-Dimensional Sonic landscapes and the most Badass Acid Lines in this Universe. After releasing EPs on Timewarp and Goa Records and on a couple of compilations, he is back with extra-terrestrial Acid infused Goa trance. Enjoy this wonderful Goa trance journey https://www.facebook.com/Themaniac604/ https://soundcloud.com/themaniac1


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Welcome to the Ida Grove Public Library!
The Ida Grove Public Library got its start in 1908. The Women's Christian Temperance Union sent out five hundred invitations asking everyone to bring a book or two for the library. They thought this movement would result in the erection down town of a large and suitable building. The ladies of the Civic Club joined forces with W.C.T.U. in opening a public library and reading room in the old Women's Christian Temperance Union Hall just south of the opera house. About $100 was spent in buying new books. The library was kept open two afternoons and evenings a week all winter. From this the library steadily grew and prospered. Expenses of the library were paid by public subscription. Books were donated by the Civic Club and other citizens. The first trustees of the Ida Grove Library were Dr. G.C. Moorehead, Robert Lipton, Mrs. Wm Wilcox, and Alex Hartley. The first Librarian was Mrs. Anna Moorehead Goodrich.
In 1910, the city council leased the east room of the city building upstairs to the library association. The association payed $1 a year rent and paid for heat, light, water and necessary repairs during the existence of this lease.
In 1921, 6159 books were loaned out, more than half of which were juvenile books. In 1922 the library trustees met with the city council to discuss the library situation. The council voted to submit the vote to the people as follows: "Shall the city of Ida Grove accept the present library as a free public library, the same to be maintained by the said city as provided in sections 727 & 732 of the 1913 supplement to the Code of Iowa?"
In 1922 circulation was free to the public after the city voted a one and one-fourth mills tax for library support. In 1937 the library had 4904 books. In the Spring of 1978, the public library moved to it's present location on second street.
Today the library offers books on CD, DVD's, video's, magazines, newspapers, large print and regular print books. The library has 5 public computer stations.

domain: library


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- People stop taking calls if you deliver a flop: Abhishek BachchanChinkara poaching case: Salman thanks fans for prayers, supportAkshay had a ball shooting cameo for 'Dishoom'Iulia Vantur celebrated birthday with Salman KhanICW 2016: Rohit Bal adds Russian touch to India fashionB-Town hails Salman's acquittal in poaching casesKangana turns Mughal empress for at the FDCI India Couture Week 2016Why not: Kangana on getting married more than once'Madaari': A heartbreaking wake-up callVijay Raaz did 'Mission Tiger' for free
The decision of the International Olympic Committee to allow each federation to assess the
Neha Dhupia wants simple wedding Last Updated : 13 Apr 2013 05:33:54 PM IST Actress Neha Dhupia displays a jewellery collection during the India International Jewellery Week 2013 in New Delhi on Saturday.
After having tied the knot onscreen several times, actress Neha Dhupia now wants to have a simple wedding in real life.
"I am not quite sure if I want to have a grand wedding. In the film industry we are getting married every two months, be it in the media, in films or on the ramp.
"I feel that the day when I actually get married, I will be too bored and end up in a very simple and plain outfit for the D-day," Dhupia said.
The 32-year-old actress walked the ramp at the ongoing India International Jewellery Week for Gitanjali's Wedding Cranival designs and looked like a bride in a Pam Mehta peach and green lehenga, which she teamed up with a kundan necklace, earrings, maangtika and a statement ring.
"This is the first time I have worked with Pam and I must say I love what I am wearing. I have had a long standing association with Gitanjali and I just love the designs," she said.
Asked what she would choose on her D-day, Dhupia said, "My wedding outfit will depend on where I am getting married, if the weather is too hot and cold. I don't want to be decked up in jewellery from top to bottom because there is also the heavy lehenga, elaborate make-up. I would not want to be weighed down by the jewellery. I want to be comfortable on my wedding day."
Dhupia will be next seen in Jimmy Shergill's Punjabi production 'Rangeelay', which is slated to release on May 16. Beisdes that, the actress is also doing Renzil D'Silva's 'Ungli', which also features Sanjay Dutt, Emraan Hashmi and Randeep Hooda.
Source: IANS, Other Agencies, Staff Reporters
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Community To Hold Caravan Through East Los Angeles Celebrating the 50th Anniversary of The Chicano Moratorium
For Media Planning: Saturday, August 29, 2020
Contact: Jorge H. Rodriguez – 310-429-1300 | email: email@example.com
Lupe Carrasco-Cardona – 323-490-9575 | email: firstname.lastname@example.org
Fifty years ago, at the height of the Chicano Movement and the U.S. involvement in the Vietnam War, over 30,000 Chicano activists gathered in East Los Angeles organizing the Chicano Moratorium, a march to protest the war and demand dignity and respect for their communities. Chicanos were overrepresented in those who were killed in combat overseas while their communities at home were consistently neglected and denied basic resources such as quality school, housing, and access to good jobs. The resulting backlash against the march by local law enforcement resulted in the injury of many peaceful participants and the death of Latino reporter Ruben Salazar after a tear gas canister struck him in the head.
Decdes later, the struggle for social and economic justice in Chicano communities across the U.S. continues. Income inequality is at an all-time high. The Coronavirus pandemic has further exposed the gaps in U.S. safety net programs as Latinos are overrepresented in the amount of COVID-19 related deaths. The death of young people of color at the hands of law enforcement continues to be a tragedy that has rocked our nation to our core bringing thousands out to the street demanding an end to the systemic oppression of communities of color.
On the fiftieth anniversary of the Chicano Moratorium, thousands of community members will take to the streets of East Los Angeles once more to commemorate this historic event and to highlight the need to continue the fight to end hatred and inequality in our communities.
WHEN: Saturday, August 29, 2020, at 10:00 am
WHERE: Caravan will assemble at 10:00 am in Pico Rivera at the intersection of Whittier Boulevard and Gregg Road. The caravan will continue down Whittier Boulevard to Ruben Salazar Park – 3864 Whittier Blvd, Los Angeles, CA 90023.
- Ron Herrera – President of Los Angeles County Federation of Labor,
- Lupe Carrasco – Cardona UTLA Member/Co-Chair of 50th Chicano Moratorium
- Jorge H. Rodriguez – Co-Chair 50th Chicano Moratorium
- Gloria Arellanes – Original 1970 Chicano Moratorium Committee Member
- Rosalio Munoz – Chair of 1970 Chicano Moratorium

domain: politics


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Wayanad - Places To See| October 7, 2012
Wayanad Wildlife Reserve: This famous park is easily accessible from all over the state. Elephants, bison, spotted deer, tiger, leopard, bear and wild boar can be spotted here. Best time to visit would be from June to October. The park remains open from 6 am to 9 am and from 3 pm to 6 pm. Park authorities charge fee for camera and guides. One can also hire a jeep to explore the park.
Pookot Lake: A very beautiful picnic spot and a place to relax in a serene backdrop. The area surrounding the lake has an aquarium, children’s park and a small emporium selling handicrafts and spices.
Kuruva Island: Situated on a tributary of the river Kabani, this is a calm place for to picnicking with family. It is an isolated place with serene environment and evergreen forests. You will get to see a number of uncommon species of birds, herbs and trees.
Pazhassi Raja Tomb: It is a memorial for Pazhassi Raja who was considered the lion of Kerala. Pazhassi Raja led guerilla-type warfare against the British for independence.
Sulthan Bathery: A small, charming town, it was originally called Ganapativattom. Its name was changed after Tipu Sultan of Mysore captured the place. A battery of the Sultan’s artillery was stationed here and hence the name.
Waterfalls: Kalpetta region of Wayanad is famous for various waterfalls, the most charming and famous being Soochippara Waterfalls, Meenmutty Falls and Kanthapara falls. Panchateertha, situated Kanthapara falls. Panchateertha, situated close to Thirunelly temple, is also famous. The word Pancha means five and teertha represents rivers. It is believed that these five rivers met at a point and hence acquired this name. Though, now this place has dried up.


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The Amazon Rainforest in particular is known as ‘the Lungs of the World’ because it sucks up global emissions of carbon dioxide and about 20% of earth’s oxygen is produced by the Amazonia not just that, more than half percent of the earth’s rainforest is in the Amazon, it is to the course that it’s even more heartbreaking that the world isn’t paying attention to our energy source.
The majority of the forest is contained within Brazil, with 60% of the rainforest, followed by Peru with 13%, Colombia with 10%, and with minor amounts in Venezuela,Ecuador,Bolivia, Guyana,Suriname and France .
The forest is inhabited by rare animals and we wonder if this fires wouldn’t lead to the extinct of these unique species of animals and green houses. With the continuity of global warming,its is our duty it protect our earth.
The Amazon is protected by tribe leaders and sometimes illegal loggers gain access to the forest carrying out serious deforestation activities illegally and since the wildfire, smog has been all over nearby cities in Brazil .
While the Brazilian government have done the best of their capabilities to hold the fire in check. Lightening is known to be the cause of major forest fires in the Amazon but in this case the fire has been faulted to actions of illegal loggers and farmers.
If we are to beat global warming, even at the tiniest bit we need the Amazon forest for it’s great carbon store to reduce global warming and the 20% oxygen for plant and animal lives.
Forest fires in the Amazon has been on for 3 weeks and the media has no coverage of it as it should.
Thousands of wildfire have been burnt to death alongside burnt trees.
This was the beautiful rainforest before the fire … 👇


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As busy and as alive Manila is during the daytime with all the business, tourists, and social/cultural events happening, Manilaâ€™s Nightlife is even more vibrant with all its international bars, world class restaurants, lounge night clubs, casinos, discos, concerts, and parties! Drink, dance and party the night away! The Oasis Park Hotel is a budget friendly business hotel in Manila that is only minutes away from the Manila Nightlife location.
Formerly known for its bars and night clubs, Malate is now known for its world class restaurants and international bars. Still one of the best social areas in Manila, it is frequented by tourists, business people and celebrities who want to relax and have a good time.
Some of the best and modern international bars, world class restaurants and lounge night clubs can now be seen in the business center of Metro Manila, Makati. Highly urbanized and thriving, Makati is home to some of the best shopping malls, night clubs and hang out spots. Expats, business people, foreigners and tourists will find almost anything they need and want here.
Quezon City also boasts its great entertainment spots and night out fun. Eastwood City is the â€ścityâ€ť within Quezon City. Featuring a complete modern business and leisure community, it has charmed many tourists, foreigners, socialites and business people to its numerous trendy bars, great value restaurants, shops, cinema house and the beautiful ambience. Manila Nightlife is very much alive here.
One of the four original cities in Metro Manila, Pasay City is an assortment of business, commercial, cultural and entertainment sites. This is where the Ninoy Aquino International Airport is located and is adjacent to the City of Manila. SM Mall of Asia, touted as one of the biggest malls in the Philippines and Asia, is a place for shopping, dining, relaxing and having fun. At the back of the mall is a popular drinking and dining strip by the Manila Bay, complete with bars and restaurants. It is now a top destination for tourists, business people, expats, and locals in Manila.
Book a hotel room at the Oasis Park Hotel Manila today and we'll assist you in finding the best places in Manila's nightlife and make sure you'll have a great time. Going all out to party or hanging out on a budget, the Oasis Park Hotel will take care of you. Our hotel room rates are very affordable which is accompanied by complete hotel facilities and amenities.


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Authorities identify woman who died after being struck by NJ Transit train in Fair Lawn
FAIR LAWN — A Hoboken-bound NJ Transit train struck and killed a woman near the Broadway station Tuesday morning.
The Bergen Line train had stopped halfway into the station as police investigated the incident, which occurred about 9:10 a.m. behind the Parisi Speed School on Banta Place. The train hit Yelena Gorovits, 47, of Fair Lawn who was trespassing at the time, said Nancy Snyder, a transit spokeswoman.
The incident occurred about 400 feet north of the Broadway Station.
The engineer, who was not identified, told investigators that he blew his horn and applied his emergency brake after seeing a trespasser run from the north side of track 1 to track 2, Snyder said.
Gorovits then faced the train and made no attempt to clear the tracks, Snyder said.
The engineer, who was shaken by the incident, was taken to The Valley Hospital in Ridgewood for observation, Snyder said.
About 200 people were on the train when the accident occurred. None of the passengers was injured.
Steven DeCoveny of Fair Lawn was waiting on the platform when he looked down to see the train coming. He said he saw no one on the tracks when he first got to the platform, but seconds later, he heard the train’s horn blast and saw a woman standing on the tracks.
DeCoveny said she did not try to jump out of the way and was hit straight on. He commended the engineer’s quick reflexes. “This person just appeared out of nowhere,” he said.
The Bergen Line trains were running with delays up to an hour for about two hours, and some Bergen Line trains were diverted to the Main Line.


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Jane Ann Alston Ceurvorst, 77, died Sept. 17, at home.
She was born Feb. 7, 1942, to the late John and Ruth Alston, in Davenport, Iowa.
Jane was survived by, sons, Joseph (Shelly) Ceurvorst, of Golden, Colo., John (Tami) Ceurvorst, of Los Angeles, Calif., Jeffrey (Erin) Ceurvorst, of Denver, Colo., Gerard (Heather) Ceurvorst, of Greer, S.C.,; daughter, Jane (Chris) Ceurvorst-Darnell, of Fort Myers, Fla.; grandchildren, Alexandra and Garrett Ceurvorst, Kaileigh, Cassandra, Katrina, and Caedmon Ceurvorst, Madeline and Courtney Ceurvorst, Bailey, Gabriel, and Colin Ceurvorst, and Ethan Ceurvorst; and sister, Mary Longer, of Fort Myers, Fla.
Funeral service will be held at 10:30 a.m. Oct. 5, at Central United Methodist Church, 801 S. Hayne St., Monroe, NC, 28112. Burial service will be held at 11 a.m. Oct. 26, at Thornber Cemetery, N. Co Rd 1200, East of Nauvoo.
Schmitz-Banks and Beals Funeral Home is assisting with local arrangements.
To leave a condolence visit Jane Ann’s obituary at sbbfuneralhome.com.


Jurrah set for AFL return this weekend
Demons forward Liam Jurrah has been picked for Saturday's match with Sydney on the same day as additional charges were laid against him over an alleged attack in Alice Springs
Liam Jurrah has been picked by Melbourne for Saturday's match with Sydney at the SCG.
This will be the forward's first game of the season for the Demons after coming into the season with a wrist injury.
The news comes on the same day as Jurrah had four more charges levelled at him following an alleged attack in Alice Springs.
Jurrah appeared on a video link from Melbourne as the prosecutors laid out their extra charges.
Four more charges have been laid against AFL star Liam Jurrah who was already facing charges of causing serious harm and being armed with an offensive weapon in an alleged melee.
The charges relate to an alleged machete attack on a man at Little Sisters town camp in Alice Springs in March.
In an Alice Springs court yesterday, the prosecution added four charges of aggravated assault to the case against Jurrah.
Jurrah, 23, appeared before the court via a video link from Melbourne.
Magistrate John Birch has set July 23 as a date for the committal hearing, at which time Jurrah will have to appear before the court in person.
Defence lawyer John McBride applied for a variation of bail conditions so Jurrah can play for Melbourne in a match against Port Adelaide in Darwin on July 21.
After earlier saying he did not usually vary bail conditions for personal matters, Mr Birch later approved the application.
The magistrate ordered Jurrah to travel to Alice Springs immediately after the match for the committal hearing.
Jurrah hails from Yuendumu, 260 kilometres north west of Alice Springs.
People from Jurrah's Warlpiri group have been involved in a bitter and sometimes bloody feud between two families that has divided the community.
- 15-year-old William Weir from Ammaroo Station has cleaned up at the Alice Springs Show
- Geoff Miers is one of the horticulture stewards at the Alice Springs Show.
- Thirty years ago Tom Vandeler was a South Australian grain and sheep farmer. These days he trains pigs to fly.
- Barkly Tablelands cattleman Chris Towne has expressed safety concerns about the shutdown of the Tennant Creek weather radar in the Northern Territory.
- The Bureau of Meteorology's (BOM) acting regional director Ben Suter said he understood frustration about the short notice but said the BOM had been c


I’m in Tokyo for CEATEC–the Consumer Electronics Show of Japan–and have been roaming the show floor and discovering nifty stuff. Some of it will show up in the U.S. eventually; some of it won’t.
Pioneer, which makes lots of aftermarket electronics for cars, is demoing a rather unusual visor. It’s an augmented reality heads-up display–Pioneer calls it an AR HUD–that uses lasers to overlay text and images on the road ahead. By doing so, it can do driving directions that use the world around you for imagery, not a digital recreation on a screen in the dashboard.
The technology should show up in commercial form in 2012, Pioneer says–in Japan at first, in a model that can be installed as an aftermarket accessory. It may be built into cars later, and should reach other countries. The price hasn’t been set yet.
(Full disclosure: I spoke at a CEATAC keynote and the show subsidized my travel costs.)


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domain: design


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With a stay at Asya Hotel Darica in Darica (Bayramoğlu Mahallesi), you'll be a 1-minute drive from Sea of Marmara and 7 minutes from Bayramoglu Bird Park. This hotel is 15.5 mi (25 km) from Viaport Outlet Shopping and 5.9 mi (9.5 km) from Eskihisar Castle.
Take advantage of the hotel's 24-hour room service. Continental breakfasts are available daily from 9 AM to 12:30 PM for a fee.
Make yourself at home in one of the 30 guestrooms featuring minibars and LCD televisions. Complimentary wireless Internet access keeps you connected, and satellite programming is available for your entertainment. Private bathrooms with bathtubs or showers feature complimentary toiletries and hair dryers. Conveniences include phones, as well as safes and desks.
Enjoy recreation amenities such as an outdoor pool or take in the view from a garden. This hotel also features complimentary wireless Internet access and concierge services.
Business, and other amenities
Featured amenities include a 24-hour front desk, a safe deposit box at the front desk, and coffee/tea in a common area. A roundtrip airport shuttle is provided for a surcharge (available 24 hours), and free valet parking is available onsite.


Compact X-ray microscopes for EUV- and soft X-radiation with spectral imaging capabilities
2006 (English)In: Advances in X-Ray/EUV Optics, Components, and Applications / [ed] Khounsary, AM; Morawe, C, 2006, Vol. 6317, 31704-31704 p.Conference paper (Refereed)
We report on a compact full-field transmission microscope (CTXM) and a scanning transmission microscope (CST'XM) developed for imaging at laboratory scale X-ray sources. The microscopes are based on zone plates for imaging in the EUV and water window region (wavelength 2.3 nm to 4.4 mn). The radiation for the full-field microscope is generated by focusing short laser pulses with an energy of 100 mJ on a 20 gm cryogenic liquid nitrogen jet. A condenser zone plate in conjunction with an aperture is used to provide monochromatic sample illumination. This allows for easy wavelength selection within the N-2,-Emission spectrum. Thus, the presented setup offers the possibility of spectral imaging. A micro zone plate generates a magnified image detected by a back illuminated TE-cooled CCD camera (1,340 x 1,300 pixel). The actual configuration provides magnifications up to 1,000x at exposure times in a range of a few ten minutes with sub-100 nm resolution. Our compact scanning microscope (CSTXM) operates with a zone plate, focusing the radiation onto a sample which is placed on a piezo driven xy-stage with 1 nm lateral resolution. Using high-harmonic radiation at 13 nm wavelength sub-micron resolution is achieved. With light at 17 nm wavelength originating from the O-VI emission line of a laser plasma source based on an ethanol jet, 500 nm structures were imaged in less than 20 minutes resulting in an 100 x 40 pixel image.
Place, publisher, year, edition, pages
2006. Vol. 6317, 31704-31704 p.
, PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE), ISSN 0277-786X ; 6317
full-field X-ray microscopy, scanning X-ray microscopy, zone plate, laser plasma source
Atom and Molecular Physics and Optics
IdentifiersURN: urn:nbn:se:kth:diva-73336DOI: 10.1117/12.679819ISI: 000241972700004ScopusID: 2-s2.0-33750594112ISBN: 0-8194-6396-5OAI: oai:DiVA.org:kth-73336DiVA: diva2:488800
Conference on Advances in X-Ray/EUV Optics, Components and Applications. San Diego, CA. AUG 14-16, 2006
QC 201202022012-02-022012-02-022012-02-03Bibliographically approved


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AUSTIN, August 15, 2014– According to Austin, Texas NBC affiliate KXAN, on Friday, a grand jury served an indictment against Gov. Rick Perry (R- Texas) in connection with the investigation into an effort to force Travis County District Attorney Rosemary Lehmberg to resign. Travis County is home to Austin, Texas.
At the center of the issue is a complaint about intimidation stemming from Perry’s threat to veto of $7.5 million in state funding to the Public Integrity Unit run by Lehmberg’s office, which came after she pleaded guilty to drunk driving and served a 45-day sentence; Perry called on her to step down. However, Lehmberg refused to resign her position, which led Perry to veto the funding for the PIU.
A grand jury was called to determine whether or not Perry broke the law when he threatened to veto the funding. As a result, indictments were issued on two felony charges. 1.) Abuse of official capacity. 2.) Coercion of public servant. If Perry were to be found guilty, he could be sentenced to a maximum 109 years in prison.
An indictment indicates the grand jury believes the state has a strong enough case to send to trial.
Here is video of Lehmber’s booking into the county jail
“The first count of abuse of official capacity. It basically charges that on the day of the veto in 2013 that Governor Perry intentionally or knowingly misused government property that had come into his custody or control,” special prosecutor Michael McCrum said Friday afternoon.
According to KXAN, McCrum told reporters that he plans to meet with Perry’s attorneys on Monday.


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The outbreak of the antibiotic-resistant bacteria known as Klebsiella pneumoniae was not made public until Wednesday, when NIH researchers published a scientific paper describing the advanced genetic technology they deployed to trace the outbreak.
This was “the proverbial superbug that we’ve all worried about for a long time,” said Tara Palmore, an infection control specialist at the Bethesda hospital.
With some 99,000 U.S. deaths attributed to hospital-borne infections annually, the NIH outbreak provides a stark case study of the dangers of the latest wave of hospital-bred bacteria and the extreme measures hospitals must adopt to stem the rising superbug tide.
The NIH Clinical Center now screens every patient transferring from another facility for superbugs, tests every patient in the intensive care unit twice a week and screens every patient monthly.
“This was our introduction to [antibiotic-resistant] Klebsiella,” Palmore said. “We hoped we would never see it.”
Clinical Center spokeswoman Maggie McGuire said the hospital did not alert the public earlier because Klebsiella infections do not trigger mandated reports to the Centers for Disease Control and Prevention like other infectious diseases do, such as HIV.
She also downplayed such outbreaks as too common to be newsworthy.
“There are . . . hospital-
acquired infections in almost every hospital in the country,” she said. “It’s happening everywhere.”
Nationwide, about 6 percent of hospitals are battling outbreaks of the class of superbugs known as carbapenem-resistant bacteria, which includes Klebsiella, said Alexander Kallen of the CDC. These bacteria usually live harmlessly in our intestinal tracts, and they pose little or no threat to patients with healthy immune systems. But in patients with compromised immune systems, the bacteria can turn dangerous, gaining an enzyme that defeats even the most powerful antibiotics. That’s what happened at NIH.
The six patients who died of bloodstream Klebsiella infections had immune systems weakened by cancer, anti-rejection drugs given after organ transplants, and genetic disorders.
The CDC detected this type of antibiotic-resistant bacteria in 2000. “Since then, we’ve seen it spread across the country,” Kallen said, to 41 states.
Later this year, the CDC is launching a program in 10 cities, including Baltimore, to watch for hospital-borne outbreaks of Klebsiella and related superbugs. CDC staff will review hospital records, Kallen said, and hospital labs will be asked to report any antibiotic-resistant bacteria to the CDC.


The diamonds of Blue Lake Sports Park will be full of runs, catches and strike outs once again this weekend when a new summer of baseball is launched.
The Mount Gambier and District Baseball League would have hoped to fly through the season without any disruptions, but a coronavirus curve ball arrived at the worst possible moment last week.
Confusion and misinformation over COVID-19 restrictions caused last weekend’s opening round to be wiped out.
Initially SA police commissioner Grant Stevens said all sport will be cancelled after a positive case in the Blue Lake City which left the MGDBL with no option, but to pull the pin.
However, the South Australia Police Updated Activities Associated Direction outlined restrictions only prevented crowds from attending venues, so sport could still go ahead.
But it was too late for the MGDBL and new president Phil Clark said it was a frustrating start to the summer.
“It started from getting messages from the government and police saying we could not play or go to training,” he said.
“We followed SA Health and then the health directions changed again and said we could carry on.
“But by then everyone had already cancelled everything from the umpires to the food and beverage orders and it was going to be too hard for everyone.
“We thought we would bite the bullet and stick with the decision to save a whole lot of running around, headaches and chasing volunteers.”
But with sport and crowds back, Clark confirmed all is in readiness for another big hitting season.
“We were disappointed to cancel the first round, but it is what it is and you have to deal with each week as it comes,” he said.
“It will be great to get back out there after we have all been hibernating.”
Cancelling games and battling the ever-changing COVID-19 environment was not how Clark would have hoped to open his term leading the MGDBA.
The 2021-22 season is his first at the helm after taking over the president’s role from Michael Summers during the winter.
The unexpected COVID-19 restrictions threw Clark in the deep end straight away, but he said he had strong support to call the shots.
“The entire committee have made things real easy for me this year,” he said.
“I have had to do a couple jobs, but so far it has not been super stressful because the season has not got under way yet for the other issues to pop up.”
Centrals is the team everyone is looking to catch after it strolled to the 2020-21 Sid Hosking premiership earlier this year with two resounding wins over South Indians.
The Magpies will carry great confidence in continuing their 90th anniversary celebrations with big names such as Dylan Chuck, Ethan Chuck, Alex Ridding and Koby Chant all expected to feature prominently.
But the likes of South, Tigers and Millicent will be determined to spoil the party.
Led by Bruce Morale, Damien O’Dine and Dean Hamilton, South Indians loom as the number-one challenger keen to get revenge.
Tigers can never be discounted having wrapped up the 2019 flag with stars such as Brayden Morton and Tyler Horrigan.
The Bandits could be a wildcard looking to build on the momentum generated from their first season in the big league and will turn to Darcy Pink for inspiration.
Clark said he is pleased with how participation numbers are tracking across the grades.
“The numbers are looking good,” he said.
“Players are still interested in the sport and we have an extra team in Division 2 which will boost a lot of moral.”
Sadly only four teams are in the fight for the Sid Hosking Division 1 premiership.
Federal was not in position to field a team in the top grade for the first time in its history due to injury and unavailability.
Clark said it will be sad not seeing the red and green team mix it with the best, but said the affected players will remain on the diamond.
“We have lost Federal due to numbers, but most of the players have joined other clubs to keep playing,” he said.
“This year a lot of players have moved out of town or are injury, so they just cannot play this season.”
After the disappointment of last weekend, Clark said the baseball fraternity is eager to get back on home plate and launch into a season which will also include extra development for the juniors.
“We are ready to go and everyone is still keen to play and excited,” he said.
“We will have some doubleheaders and byes throughout the season.
“We are also going to do a bit of junior development training led by Tracy McAdams to get them ready before the April carnival comes along.”
The season begins under lights tomorrow night with more games to follow at Blue Lake Sports Park all day Saturday.