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A Closer Look: Biological soil crusts as restoration targets in sagebrush steppe and woodland communities
By Lea Condon, Disturbance Ecologist,
USGS Forest & Rangeland Ecosystem Science Center
Across the globe, biological soil crusts – commonly called biocrusts – aid ecosystem functions like nutrient and hydrologic cycling, soil stabilization and the maintenance of albedo (the amount of light reflected by the earth’s surface). Biocrusts are a mostly photoautotrophic (creates its own food) soil surface community composed of moss, lichen, cyanobacteria, algae, and fungi (fig. 1). Biocrusts occur in all plant communities across arid and semi-arid ecosystems in the western U.S.
Figure 1. An example of biological crusts from the Great Basin, U.S.A.
Across the sagebrush steppe of the Great Basin, biocrusts help to increase the ecosystem’s resistance to invasive species, especially in the presence of fire and grazing. Mosses, lichens, and perennial grasses are associated with reduced cover of cheatgrass (Bromus tectorum L.). Similarly, when looking at both vascular plants and morphological groups of biocrusts along disturbance gradients of fire severity, grazing intensity and invasion by cheatgrass, perennial grasses increase in cover with low to moderate levels of fire severity and grazing intensity. However, morphological groups of biocrusts are abundant along different portions of these disturbance gradients. Some mosses are lost following fire, but are more tolerant of grazing. Losses to the cover of tall mosses (mosses over 1 cm tall) – such as Syntrichia ruralis (Hedw.), F. Weber and D. Mohr – appear to foreshadow the loss of perennial grasses. Lichens are generally sensitive to grazing, but are somewhat tolerant of fire.
There is a growing body of research on active restoration of biocrusts. Dryland mosses have been restored with regular increases in cover of 30 percent a year, and the same methods have been successful in the semi-arid intermountain grasslands of western Montana and the Colorado Plateau. However, successes with the lichen component have been limited to ruderal groups – species that are first to colonize after disturbance such as Cladonia sp., or material that has been lifted from one place and laid down on top of the soil surface in another (also known as salvage efforts). Many ruderal species and groups are present with disturbance or quickly recover following the cessation of disturbance. In these cases, it is not clear if active restoration is necessary or beneficial to ecosystem processes.
The levels of restoration success are dependent on the restoration goal. SageSTEP addresses many common restoration goals across the region, such as recovery of native vegetation, hydrologic function and sagebrush obligate birds. SageSTEP has previously included the response of biocrusts – as a single entity (moss + lichen), to fuel reduction treatments within the sagebrush-cheatgrass and woodland sites, and separately as mosses, lichens and cyanobacteria on the Onaqui woodland site for responses to prescribed fire. The first three years of data from across the woodland sites indicate that mowing treatments are not as detrimental to biocrusts, (moss + lichen), compared with prescribed fire (fig. 2). However, sites level difference indicate that not only did cover of biocrusts vary by site prior to treatment, but the post-treatment responses varied as well (fig. 3). For example, compare the crust response at Bridge Creek v. Onaqui, through three years of time – while both fire and mechanical treatments caused a decrease in crust at Onaqui, only fire had that effect at Bridge Creek. And at Scipio crusts declined in all plots through time. Further work is needed to understand these kinds of site-level differences.
Click on images to get a closer look
Figure 2. Short-term response of biological crust (moss + lichen) to fire and mechanical treatments, averaged over 10 woodland sites (mean +/- 95% confidence intervals).
Figure 3. Short-term response of biological crust (moss + lichen) to fire and mechanical treatments for 10 woodland SageSTEP sites (mean +/- 95% confidence intervals).
The response of biocrusts to fuel reduction treatments sets this interesting and often overlooked vegetative group, as another restoration component to consider if reduced cover of cheatgrass is a management goal.
Want to know more about biological soil crusts?
Bowker MA, Antoninka AJ, Chuckran PF (2019) Improving field success of biocrust rehabilitation materials: hardening the organisms or softening the environment? Restoration Ecology doi.org/10.1111/rec.12965
Condon LA, Pyke DA (2016) Filling the interspace–restoring arid land mosses: source population, organic matter and overwintering govern success. Ecology and Evolution 6, 7623-7632.
Condon LA, Pyke DA (2018a) Fire and grazing influence site resistance to cheatgrass invasion 520 through their effects on shrub, bunchgrass, and biocrust communities. Ecosystems 21, 1416-1431. doi: 521 10.1007/s10021-018-0230-8. 522
Condon LA, Pietrasiak N, Rosentreter R, Pyke DA. (2019) Passive restoration of vegetation and biological soil crusts following 80 years of exclusion from grazing across the Great Basin. Restoration Ecology doi.org:10.1111/rec.13021
Condon LA, Pyke DA (2018b) Resiliency of biological soil crusts and vascular plants varies 523 among morphogroups with disturbance intensity. Plant and Soil 433, 271-287.
Miller RF, Ratchford J, Roundy BA, Tausch RJ, Hulet A, Chambers JC. (2014) Response of conifer-encroached shrublands in the Great Basin to prescribed fire and mechanical treatments. Rangeland Ecology and Management 67(5), 468-481. doi.org/10.2111/REM-D-13-00003.1
Pyke DA, Shaff SE, Lindgren AI, Schupp EW, Doescher PS, Chambers JC, Burnham JS, Huso MM. (2014) Region-wide ecological responses of arid Wyoming big sagebrush communities to fuel treatments. Rangeland Ecology and Management 67(5), 455-467. doi.org/10.2111/REM-D-13-00090.1
Slate ML, Durham RA, Pearson DE. (2019) Strategies for restoring the structure and function of lichen-moss biocrust communities. Restoration Ecology doi:10.1111/rec12996
Warren SD, St. Clair LL, Johansen JR, Kugrens P, Baggett LS, Bird BJ. (2015) Biological soil crust response to late season prescribed fire in a Great Basin juniper woodland. Rangeland Ecology and Management 68, 241-247. doi.org/10.1016/j.rama.2015.03.007
Warren SD, St. Clair LL, Leavitt SD. (2018) Aerobiology and passive restoration of biological soil crusts. Aerobiologia 35, 45-56.
Dpt. Fisheries & Wildlife
Oregon State University
Corvallis, OR 97330
GB Rangelands Research
USDA Ag. Res. Service
Reno, NV 89512
(775) 784-6057 ext. 233

domain: literature


text:
This Rustic Apple Crumble Crostata combines two of my family's favorite apple desserts; an apple pie and an apple crumble. It's both casual and rustic but still has a lot of pizzazz.
I originally shared this recipe in 2015. I've recently updated this post with additional information, including a recipe card complete with nutritional information, but the recipe itself (and the photos) have remained unchanged.
**Please Note - The printable recipe card with detailed instructions, complete ingredient measurements, and nutritional information for making this easy rustic apple crumble crostata is available at the end of this post. Or you can hit the "jump to recipe" button (found above) to be taken directly to the recipe.
The ingredient list:
**Note - The following lists of ingredients plus kitchen tools and equipment contain affiliate links (highlighted in blue) for your consideration and shopping convenience. #CommissionsEarned
- 2 - 9" round pie crust (homemade or store-bought)
To make the filling:
- pink lady apples
- granulated sugar
- brown sugar
- lemon juice
- pure vanilla extract
To make the crumble:
- quick-cooking oats
- granulated sugar
- brown sugar
- ground cinnamon
- all-purpose flour
- pure vanilla extract
To make the icing:
- confectioners sugar
- pure almond extract
Kitchen tools and equipment needed:
- large mixing bowls
- apple peeler
- chef's knife
- paring knife
- cutting board
- rolling pin
- measuring cups and spoons
- nutmeg grater (optional)
- pastry blender (optional)
- piping bag, parchment triangle, or sandwich-sized zip-top bag
- baking sheets
- baking spray
- extra large spatula (cake lifter)
- cake boards (for storing and freezing)
- serving tray
- cake plate
- pie server
How this recipe came to be:
My parents were coming for dinner, so I wanted to make something simple for dessert with ingredients I already had on hand in my pantry and fridge.
I took out the package of frozen pie dough and let it thaw on the counter as I pondered in my head what I would do with it.
As I was peeling the apples that I'd had in my fridge the idea of making a crumble topping in order to dress up this rustic crostata popped into my head, and I ran with it!
Sorry that I don't have a photo of a slice of the crostata for you, but because I was bringing them to feed family and friends, it didn't seem right. Forgive me? LOL
Why this recipe works:
Everyone will think you went to a lot of trouble to make this casual yet impressive dessert but only you need to know that they only take moments to put together using everyday basic baking ingredients.
How many rustic apple crumble crostatas will this recipe make?
As written this recipe will make two crostatas.
However, should you want to make one crostata, cutting the recipe ingredient amounts in half shouldn't be a problem.
Can this rustic crostata be frozen after baking?
Make sure to cool the completed crostata or crostatas completely before wrapping them well in a few layers of plastic wrap.
*Note - before wrapping, you may want to place the crostata on a cardboard cake round for stability before freezing.
They can then be stored in the freezer for up to 2-3 months.
Can other types of apples be used to make these apple crostatas?
Yes, but make sure you use apples that hold their integrity and won't fall apart or get mushy during baking.
Best varieties of apples for baking:
- Matsu (Crispin)
- Pink Lady
How long will the apple crostatas keep in the refrigerator?
Wrapped well, you can keep these in the refrigerator for up to 5 days.
Serving suggestions for rustic apple crostatas:
Serve them hot out of the oven, at room-temperature, or even cold right from the fridge. The choice is yours and they're all equally delicious, if I do say so myself!
Topping suggestions for an apple crumble galette:
Whipped Cream, ice Cream (your favorite flavor), caramel drizzle, chocolate drizzle, confectioners sugar dusing, strawberry sauce...where will your taste buds take you?
Some step-by-step photos for making this Apple Crumble Crostata:
- Prepare the apple filling by adding the peeled and sliced apples, brown sugar, granulated sugar, cinnamon, lemon juice, vanilla extract and salt in a large bowl. Mix well to combine.
- In another large bowl, mix the crumble ingredients (oats, brown sugar, granulated sugar, butter, flour, vanilla extract, cinnamon and salt) together to form crumbs. Set aside.
- Use a rolling pin to gently roll the pie dough to increase the circle's circumference by about ½ inch. Transfer the rolled pie doughs onto two baking sheets that have been sprayed with baking spray.
- Place half of the sliced apples into the center of each pie dough, making sure to leave an approximate 2" border. *Note - Make sure to drain the liquid from the apples before adding them onto the pie crust if needed.
- Fold the border crust over the apples in sections. *NOTE - Don't forget that this pie is rustic, so do not worry that it's not perfect.
- Top each crostata with half of the crumble topping and bake in a preheated oven for 40-45 minutes or until the juices start to bubble.
- Remove to a cooling rack and cool completely.
- In a small bowl, whisk together the icing ingredients until smooth. Drizzle the top of the cooled crumble topping with the icing, or fill a piping bag with the icing and pipe stripes of icing all over each crostata.
- Transfer the cooled and iced crostata using a large, wide spatula to serving platters or cake plates.
What is the difference between a crostata and a galette?
Crostata is an Italian word, and galette is French.
They both basically mean "free-form pastry," and are interchangable.
Additional apple recipes (sweet and savory) you'll also enjoy:
*Apple Pie Wedges (shown below and featured in the video)
You’re going to love how easy and delicious these apple pie wedges are to make…and eat. With store-bought or homemade pie crust, apple pie wedges are utterly delicious!!
*Pork Meatballs with Apples and Onions (not shown)
Pork Meatballs with Apple and Onion are tender meatballs made with ground pork, grated apple, onion, and seasonings. The meatballs are browned in a skillet and finished in the oven, along with a sweet and savory pan sauce that will knock your socks off.
*No-Knead Apple Yeast Bread (shown below)
This delicious and sweet No-Knead Apple Yeast Bread is moist and flavorful. It’s loaded with lots of fresh apple chunks and plenty of warm cinnamon. The aroma of it baking in the oven is something you need to experience!
Fall’s bounty of flavors and colors shine brightly in this delicious and easy dish of Roasted Sweet Potatoes, Brussels Sprouts, Bacon, and Apple.
* Apple Crumble Crostata (shown below) THIS IS THE PLACE!!
Below is the printable recipe card along with the exact ingredient measurements and nutritional information for making today's rustic yet impressive Apple Crumble Crostata.
⭐If you've made this recipe, or are excited to make this recipe, I'd appreciate it if you'd take a moment to leave it a star rating on the recipe card, along with a comment if you're so inclined. Thank you. 🙂
Apple Crumble Crostata
FOR THE CRUST:
- 2 9" round pie crusts homemade or store-bought
FOR THE FILLING:
- 4 Pink Lady apples peeled, cored, and thinly sliced
- 2 tablespoons fresh lemon juice 1 lemon
- ½ cup granulated sugar
- ¼ cup brown sugar
- 2 tablespoons ground cinnamon
- ⅛ teaspoon ground nutmeg
- ⅛ teaspoon salt
- 1 teaspoon pure vanilla extract
FOR THE TOPPING:
- 2 cups quick-cooking oats
- ¼ cup brown sugar
- ¼ cup granulated sugar
- ⅛ teaspoon salt
- ½ teaspoon ground cinnamon
- 3 tablespoons flour
- 1 teaspoon vanilla
- 6 tablespoons butter cut into pieces
FOR THE ICING:
- 1½ cup confectioners sugar
- 1 tablespoon milk
- 1 teaspoon almond extract
- -Preheat oven to 350-degrees Fahrenheit.
TO MAKE THE FILLING:
- -Place filling ingredients in a large bowl and toss to mix thoroughly.
TO MAKE THE TOPPING:
- -Place the topping ingredients in a large bowl and use your hands (or a pastry blender) to work the butter into the flour, sugar, and oats to form a mixture that resembles small peas or coarse crumbs.
TO PREPARE THE CRUST:
- -Use a rolling pin to gently roll the pie dough to increase the circle's circumference by about ½ inch. Transfer the rolled pie doughs onto two baking sheets that have been sprayed with baking spray.
TO ASSEMBLE THE CROSTATAS:
- -Place half of the sliced apples into the center of each pie dough, making sure to leave an approximate 2" border. *Note - Make sure to drain the liquid from the apples before adding them onto the pie crust if needed.
- -Fold the border crust over the apples in sections. *NOTE - Don't forget that this pie is rustic, so do not worry that it's not perfect.
- -Top each crostata with half of the crumble topping and bake in a preheated oven for 40-45 minutes or until the juices start to bubble.
- -Remove to a cooling rack and cool completely.
- -In a small bowl, whisk together the icing ingredients until smooth. Drizzle the top of the cooled crumble topping with the icing, or fill a piping bag with the icing and pipe stripes of icing all over each apple crumble crostata.
- -Transfer the cooled and iced crostata using a large, wide spatula to serving platters or cake plates.
- -Cut into wedges, serve and enjoy!
I sincerely hope you've enjoyed today's easy apple crostata with an oat crumble topping as much as I've enjoyed bringing it to you!

domain: food


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The Laryngoplasty procedure is giving patients their voice back – one injection at a time.
For patients with paralyzed vocal cords, it can be difficult or impossible to communicate with their friends and loved ones. Until now, the remedy for this condition has been invasive surgery. The laryngoplasty procedure avoids anaesthesia, prolonged hospital stays, and lengthy recovery periods. It is an injection that results in the cords being able to touch each other again and produce sound. Patients can walk in to the appointment, and walk out of the hospital within an hour, with almost immediate results.
In some circumstances, the laryngoplasty procedure can give terminally ill patients who would be otherwise too frail for a surgical procedure their voice back, allowing some patients the ability to communicate freely with their loved ones in their final days and weeks.

domain: medical


Welcome back for week three of the “Rock Your Socks” composer series! In case this is your first week on my blog, make sure to check out my previous blog posts about Amy Beach and Fanny Mendelssohn. Both were truly incredible composers that few have heard about. Throughout the course of my blog, I’ll be inserting the occasional four-week series about different composers you should know about. This month, I’ve been writing about female composers in history in honor of Women’s History month.
To introduce the next composer of this series, I have to do a little bit of a preface. Without trying to diminish neither Fanny Mendelssohn’s nor Amy Beach’s incredible compositions, I have to admit that their particular styles are not my first choice when I want to listen to classical music. For the most part, I have a few favorite pieces from each that are on my classical music playlist. Cecile Chaminade, however, makes the top five in my list of favorite composers. Her flute and piano compositions are not only impressive when it comes to the technical elements of the music, but each piece feels like it takes the listener on an emotional journey or tells a story. (And, in fact, much of what she wrote was for ballets and operas, so I guess I’m not far off). They have almost a cinematic quality to them that keeps me riveted through the whole piece, and leaves me wanting more at the end! So, basically, Cecile Chaminade knows how to use magic. There’s no other explanation.
Now for the facts: Cecile Chaminade was born in France in 1857. She received her first music lessons from her mother, who was an accomplished pianist and singer. Cecile began composing at seven years old. When she was of age, her father would not allow her to study at the Paris Conservatory of Music, but she was allowed to study privately with Conservatory professors. She later toured the United States, (including performances at the historic Carnegie Hall) and Europe, frequently performing for Queen Victoria. She was the first female to be awarded a position in the the Legion of Honor, and even inspired women across the United States to form musical societies called “Chaminade Clubs.” Throughout her life, Chaminade is speculated to have written approximately 400 pieces, publishing around 200 of them during her lifetime. Cecile died on April 13, 1944
If you’re looking for a fantastic piece from the late Romantic period, I would recommend Chaminade’s Arabesque no. 1, Opus 61. You will not regret it. The motive and sequences she uses throughout elicited an emotional response when I first heard it, (my eyes fogged up ok? It happens. I'm sure it was just allergies or something....) Really though, it is so, so beautiful. For any piano families reading this blog, I strongly encourage you to check out more of Chaminade’s work, maybe share some facts you learned about her with your family, and spread the word! There really are very few who know about these accomplished composers, and I would love to do my part in helping others discover their amazing contributions to classical music!
Thanks so much for joining me as I geek out about these stunning composers. I hope you can join me next week for the fourth and final installment of this little series. See you next time!


In the past few years, Ugu District has attracted a significant number of tourists during the festive season – and according to local establishments, the 2023 figures indicate a similar pattern.
This bodes well for the KZN South Coast with the Easter holidays around the corner!
“The occupancy rates are a very positive indicator of our growing tourism economy, and the fact that the KZN South Coast is offering visitors what they want – a nature-based experience which is affordable for the whole family,”
commented Deborah Ludick, Acting CEO of South Coast Tourism & Investment Enterprise (SCTIE).
“Reports from our bigger tourism establishments between Christmas and New Year indicated 100% occupancy rates. This is particularly significant considering the high rainfall experienced over December, something we believe is a testament to our diverse tourism assets and welcoming environment.”
Ludick said KZN South Coast’s subtropical climate meant visitors did still get to enjoy the region’s Blue Flag Beaches and tidal pools – the highest number in KZN – as well as its world-class dive sites and riverside water sports.
“While many come for our beaches, visitors soon realise they are spoilt for choice with everything from hiking excursions and extreme adventures in the hinterland, to township tours and cultural experiences. The Golf Coast, as it’s known, is a golfer's dream with 11 courses on offer. There is also the latest Wellness Way route and the Mission Tourism Route catering to all tastes in the Paradise of the Zulu Kingdom.”
Tourism establishments note positive performance
Corné Alberts, Group Marketing Manager for ANEW Hotels & Resorts said ANEW Resort Ingeli Forest near Harding experienced a ‘positive performance’ with the majority of guests visiting from inland provinces.
“Despite the lingering effects of the pandemic, we observed a steady year-on-year increase, indicating a strong post-COVID recovery with an increased occupancy rate in 2023 compared to previous years. However, it's essential to acknowledge that the growth has been somewhat tempered by factors such as increased fuel and living expenses.”
She noted the natural beauty of the KZN South Coast, as well as its beaches, diverse wildlife, and scenic landscapes, as ‘major drawcards’:
“Additionally, the region's cultural heritage, vibrant local communities, and a range of recreational activities such as water sports and nature trails contribute to its appeal. Feedback from guests has been overwhelmingly positive, with many expressing satisfaction with our services, facilities, and the overall experience. The positive sentiment bodes well for future visits, indicating a likelihood of return visits.”
Leonard Gomo, General Manager of Margate Sands also noted an increase on previous years’ occupancy rates:
“We enjoyed a 6.5% increase from last year’s occupancy of 89.18%. The majority was retained business with visitors happy with the assurance that load-shedding and water shedding would not ruin their holidays, plus the abundance of Blue Flag beaches found here.”
Michael Bester of One Way Productions, tasked with running the free-to-attend Let's Go Summer 2023/24 programme at KZN South Coast beaches, said the weather was challenging on days, but visitors still came out in their numbers to participate.
“With the current economy and our tourism offerings, we are a first-choice holiday destination. Our gorgeous beaches, golf courses, affordable accommodation establishments and great entertainment venues put us at the forefront of holiday choices. Our beaches were clean and open over the summer season, and even with challenging weather conditions, people could still have an amazing holiday on the KZN South Coast,”
#uncover #uncoverkznsouthcoast #uncoveradventure #kznsouthcoast


Surplus Computers offers the refurbished Dell OptiPlex GX Intel Pentium 4 2.4GHz Mini Desktop PC
Even with around $25
for shipping, it's the lowest total price we've seen for an OptiPlex with these specifications.
This system features an Intel Pentium 4 2.4GHz processor, 512MB RAM, 40GB hard drive, CD-ROM drive, Ethernet connection, and Windows XP Professional. A 90-day reseller warranty applies. Other notable refurbished Dell desktop deals:


text:
Contributed by Ryan Wheeler
On Thursday September 13 just after 5pm we received text, email, and phone alerts to evacuate all campus buildings. Phillips Academy responded quickly to evacuation orders, due to gas fires and explosions in Lawrence, Andover, and North Andover. Reports from town officials at the time stated that in Andover a total of 35 fires were reported with 18 fires burning at the same time. Significant damage was being reported from surrounding communities as well, and sirens and emergency vehicle were regular sights on Main Street. Subsequent reports indicate that older gas lines had been over pressurized, resulting in gas accumulation, fires, and explosions. Officials and first responders described the scene as a “war zone” and “Armageddon.”
Quiet study night at the Peabody had just begun when we received the evacuation order and several students were already in the building. They evacuated and joined their peers on the Great Lawn. All students were well cared for by their house counselors, faculty, and administrators and ultimately were able to get back into their dorms around 11:00pm. Classes were cancelled on Friday at Phillips Academy and in the other affected towns.
I remained at the Peabody until just after 7:00pm to ensure that there was no immediate danger to our collections. Rachel Manning arrived and kept an eye on things for another hour. Curator of Collections Marla Taylor was in touch with both of us. By this time utility workers had depressurized the gas lines in our vicinity and all electricity had been shut down to the towns.
Following in the wake of the recent conflagration and near total loss of the Brazilian national museum, we were extremely concerned about possible threats to the Peabody building and collections. The Brazilian fire illustrates just how susceptible cultural collections are to loss. In that case officials estimate that nearly 20 million objects were destroyed, including recordings of now-extinct Native languages, paintings and decorative arts, and other significant archaeological and ethnographic collections.
Happily the Peabody has never had gas service, so we were relatively safe, though gas can travel through the soil and invade basements. Gas lines do exist in the area and provide service to many of the homes and apartments on campus and in the vicinity. There is an access point to one line–a vault–just outside our building and I watched the utility worker depressurize this and shut it off. This site has continued to attract the attention of utility workers over the subsequent days. On Wednesday September 26 workers purged old gas from these lines in anticipation of line replacement.
This was a pretty scary emergency, especially considering the scale and scope. It’s absolutely heartbreaking to see the loss of homes in our Merrimack Valley community, as well as learning about the injuries to dozens of people and at least two deaths. Many homes and businesses in the area remain without heat and considerable numbers are out of work.

domain: news


text:
Emerald Turquoise and Coral Flower Earrings
Incredible Hand Carved Natural Coral and Stunning Emerald Turquoise with Citrines; in the Crown, above the Flower and in the Centre.
They are like something from years ago but never made until now...
I love them and have a pair myself by the same amazing artist, Lisa van Ripper.
They are so special.
Sterling Silver & Signed.
Size: over 3 inches.

domain: artistic


text:
Daisy Mae Whitelaw Drew:
How much would a Yellow Lab puppy cost?
Does anybody know any breeds?
Any advise helps!
I'm not bothered about how much I need to pay!
Thanks everybody :)
Answers and Views:
Answer by mauveme49
$ 1000 approx for a good quality pet puppy.
Answer by spanky_1992
www.petfinder.com, www.hoobly.com, www.puppyfind.com you can find prices of Golden Labs on here (if you find any). There are plenty of breeds but I don't know what breeds your looking for if their toy, small, standard, large, or X-large breeds. www.selectsmart.com/DOG/ www.dogbreedinfo.com/search.htm http://animal.discovery.com/breed-selector/dog-breeds.html
Here is a couple of websites you can take the quiz to see what breed size and what breed will fit for you! If you work almost all day, my best advice is that you don't get a toy/small breed and/or puppy. Since puppies bladders can't hold as long as an adult dog and toy/small breeds have to go outside plenty of times throughout the day. You can also look at the nearest Humaine Societies and see if there is any dogs there that you would like as well.
Answer by Rawrrr
The price of a puppy varies from breeder to breeder. If you want a list of breeders, google for some in your area, or anywhere if you are willing to fly the puppy in. I think you should do a little more research before getting a puppy.
Answer by .
From a reputable breeder, minimum $ 1000.
From a backyard breeder (never get your dog from here, it will be prone to health and behavioral issues) a couple of hundreds.
From a shelter: average $ 50 - $ 300
However, remember that whatever you pay for your dog, will not even come close to how much you have to pay in the dog's lifetime.
Answer by Lizzie
I know a bit about lots of breeds. Lab puppies vary in price. If you pay more for a good one from a *reputable* breeder, you will very likely save yourself thousands of dollars at the veterinary hospital later on, and have a healthier, better looking, and all around better pet.
You can find reputable breeders through the national breed club website online. Try a quick search for "Labrador Retriever Club of America."
Answer by Katehrox
Lots, a yellow lab is a loyal and playful dog, so they cost alot. A yellow Lab would shed alot though. Some good breeds are... terriors, collies ....
Answer by Lucy
The cost of a yellow lab varies and it depends where u get it as well !! And some adorable dog breeds are:
Cavalier King Charles Spaniel
Read other answers in the comments.
What do you think?

domain: pet


text:
Social Awareness, Campaigns & Fundraising
We are the Southampton University Affiliated Amnesty International Student group.
We're the Beekeeping Society, or BeeSoc for short, and we provide training and practical hands on experience for all levels of experience.
Black Women's Project
The Black Women's Project is committed to the personal, academic and professional growth of Black Women in higher educational institutions.
Founded at the University of Southampton, Cameroon Catalyst is a student driven charity which implements infrastructure projects in rural Cameroon which encourage sustainable development.
Consent Awareness and Sexual Health Education Society
UoS CASHES is a student-focused society aiming to campaign for better consent awareness (and sexual health education) in the Southampton student population.
Conservation Volunteers (SUCV)
We run local conservation days on Sundays, restoring ecosystems and biodiversity around Southampton and Hampshire.
CoppaFeel! UBT Southampton
CoppaFeel! Uni Boob Team works throughout the year to promote the awareness of breast cancer, particularly amongst young people.
Engineers Without Borders (Southampton)
Looking for enthusiasts from all backgrounds to change the world through engineering projects and outreach.
This society is for anybody from the university who has an interest in the Feminist movement.
Friends of MSF
We're a student group raising money and awareness for Medicins Sans Frontieres (Doctor's Without Borders).
HackaSoton is a student-led community of skillful and passionate developers and tech-lovers who look for constant improvement by putting their knowledge in practice through entrepreneurial, technical and engineering competitions, conferences and events.
Hedgehog Friendly Campus
Our aim is to raise awareness for the reduction in hedgehog population around the UK and teach students and staff how to ensure that Southampton University Campuses are hedgehog friendly, and what we can do to make our gardens and outdoor spaces as hedgehog friendly as possible.
KEEN Southampton offers free activity sessions for children and young people with additional needs.
Lawyers Without Borders
The Lawyers Without Borders Southampton Student Division is a student-run group in affiliation with the non-profit organisation Lawyers Without Borders, formed to promote and further their work.
Welcome to the LGBTQ+ Society at the University of Southampton! We welcome anyone from any gender or sexual minority, and even allies who wish to support our community.
Marine Conservation Society
The University of Southampton Marine Conservation society are a group of people dedicated to conserving our wonderful coastlines and oceans.
Mind Society exists to gather students from University of Southampton who are passionate about campaigning on behalf of Mind, the mental health charity.
Neurodiversity & Disability Society
We're a society aimed at students who identify themselves as neurodivergent or disabled, those who are undiagnosed or self-diagnosed, and any members of the community that wishes to learn more about neurodiversity and disability issues.
Sign Language Society
We promote the use of British Sign Language (BSL), offering non-professional classes for people who want to learn the language throughout Semesters 1 and 2.
Southampton Hub is a branch of the national charity Student Hubs, offering volunteering opportunities within the local community.
Southampton Robotics Outreach
SRO is a group of students who are enthusiastic about getting young people involved in Robotics, Computing, Electronics and STEM.
Student Action for Refugees (STAR)
Student Action for Refugees (STAR) works to raise awareness about and improve the lives of refugees in the UK by doing the following: Volunteer at local refugee projects; Campaign to improve the lives of refugees; Educate people about refugees and asylum; Fundraise to welcome refugees
Student Scout And Guide Organisation
Do you want to learn new skills working with young people and make lifelong friends? If so, the Southampton Student Scout and Guide Organisation (Southampton SSAGO) is for you! We are a University of Southampton society, mainly interested in volunteering in the local Scouting and Guiding community.
Vegan and Vegetarian Society
Whether you're a vegetarian, pescetarian, vegan, or an open-minded omnivore, join the Southampton Vegan and Vegetarian society to have fun and enjoy the benefits.
Widening Access to Medicine Southampton
We aim to increase diversity within Medicine and improve access to opportunities for current and future medical students.

domain: sociology


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In addition, nega tive RKIP and positive p ERK expression was observed in 18 of 26 metastatic lymph nodes obtained from patients with recurrent disorder. Our findings recommend that mixed expression may be an independent prognostic component. ERK or MEK activation success in the sequential activation of the series of protein kinases, such as Raf one, plus the up regulating protein RAS. Somewhere around 30% of all human tumours have an activating mutation in the RAS gene. Specifically, KRAS mutations are amid the most common genetic abnormalities in many kinds of human cancer, which include pancreatic cancer, colon can cer, and lung cancer. In contrast, RAS mutations have already been observed in only a smaller proportion of human gastric cancers,implicating other mechanisms during the activation of RAS signaling in gastric tumourigen esis.
B RAF mutations are additional narrowly distributed and are prevalent within a couple of specific malignancies, includ ing melanoma, papillary thyroid cancer, and very low grade ovarian cancer, but are certainly not found in gastric cancer. During the existing study, we focused on extra down stream proteins this kind of as MEK, ERK, and RAF inhibitors this kind of as RKIP, and did not measure RAS or RAF expres sion. We previously showed that substantial expression of HER1 or HER3, which are upstream selleck chemicals elements of your RAS RAF MAPK along with other tyrosine kinase pathways, was linked with bad survival in gastric cancer. Moreover, we reported that the expression of m TOR in a further pathway involving HER was linked to survi val in gastric cancer. Signaling pathways involving tyrosine kinase receptors appear to be intimately related to invasion, metastasis, and outcomes in gastric cancer. However, anticancer agents that inhibit these pathways usually are not utilized clinically, together with the exception of trastuzu mab, an HER2 antagonist.
Molecules implicated in downstream signaling pathways, such as ERK, could be targets for chemotherapy in superior or metastatic gasoline tric cancer. Smaller molecule inhibitors of your MAPK cas cade which can be created to target a variety of techniques of this pathway, this kind of as MEK inhibitor and Raf inhibitor, have entered clinical trials, but direct ERK inhibitors have however for being evaluated. Lots of pathological and molecular assays suggest that the full details gastric cancer is often a heterogeneous ailment. Nonetheless, in spite of evidence indicating that gastric cancer is charac terized by interindividual differences in tumour progres sion, histopathological features, and treatment response, a one particular dimension fits all technique to evaluation has become utilized in many studies of gastric cancer, resulting in inconsis tent outcomes. The procurement of specimens from multiple internet sites can be vital when assessing het erogeneous tumours.
We uncovered that E2F variables had been linked to Cluster 3 expression sug gesting the acknowledged ethanol dependent reduction of ionized calcium levels is acting with the transcription level to inhibit, then release entry into the cell cycle. NFB as central regulator NFB is a central regulator appearing in all but certainly one of the cluster perform analyses,indicating a amount of co regulation linking ethanol ingestion to in nate immunity and the inflammatory response. Combin ing temporal expression patterns with direct and indirect molecular interactions defined by pathway analysis, we hypothesize that NFB regulated inflammation is down regulated early and moderates as blood ethanol amounts reduce. The reducing inflammatory response outcomes from decrease levels of S100A proteins and their impact on RAGE induction of NFB, and RORA activation of NFKBIA. BMI1 is induced in an quick early res ponse and could counter the decreased inflammatory response.
Proof for that return to ordinary amounts of NFB exercise is while in the late enhance in expression PD184352 MEK inhibitor of S100A8 and Cluster one genes KLF3, UBE2D3, and PF4V1, and might be a end result of improved expression of Cluster seven gene, HMGB1 and subsequent activation of RAGE. Modulating the late maximize in NFB exercise is BAX, a further Cluster 1 gene. NFB acts as the two an enhancer and suppressor inside the MHC CII promoter,suggesting NFB regulation of the expression spike during the two Cluster six MHC CII components. BIOBASE is known as a impressive tool that complements the IPA analysis by on the lookout for conserved transcription component binding web-sites inside the GOIs and moving upstream to iden tify vital signaling pathways. Inside a 1200 bp window, various transcription things had been uncovered to bind to GOI promoters across over a single cluster.
SPI 1, ATF2, and CREB1 are just about every binding inside of genes in mul tiple but not precisely the same clusters,. ATF2 and Dovitinib JUN are both found in Clusters three and four, which have differing expression patterns general, but share a marked lower in expression from BAC1 to BAC2. The shared ATF2 CREB binding internet site is observed in Clusters two and 3 which have equivalent expression patterns, specially from BAC2 to BAC5. The p38 MAPK pathway was recognized being a central regulatory pathway for clusters one, 2, three, four, and 5. These clusters have varied expression patterns very likely reflecting the heterogeneity of cells in entire blood, interac tions with diverse other signaling molecules or tran scription elements, and also the variability of post translational modification, none of which can be detected by BIOBASE. The p38 regulated practical classes predicted by BIOBASE comprise of the innate and inflammatory immune re sponses, ubiquitination, apoptosis, and power metabolic process. One of a kind signaling pathways that may modulate p38 MAPK were predicted by BIOBASE within three clusters.
In this format, 1133 exceptional transcripts have been overexpressed in resting NK cells. Comparison of the NK cell signatures from your two platforms resulted in the widespread set of 164 genes. The genes included inhibitory NK cell surface molecules, activating NK cell surface molecules also as cytotoxic mediators. Quite a few genes encoding chemokines, cytokines and their receptors and genes involved in secretory functions were also expressed at high ranges in resting cells. We also examined a curated resting NK signature in the literature derived through the Affymetrix platform. Thirty 3 of sixty genes have been noticed in our Affymetrix derived signature. demon strating consistency inside of the individual platform. Temporal evaluation on the IL2 stimulated NK cells Self organizing maps created from spotted microarray information identified the differentially expressed genes that had at the least a 2 fold alteration in expression degree between the time factors analyzed.
The expression of your selected pathways was examined and cross validated on both platforms. Additional genes on Affymetrix microarray small molecule inhibitors platform belonging to practical groups that had been established for being differentially expressed have been integrated for much more extensive interpretation. Countless members on the TNFSF and TNFRSF were upregu lated early. Some affecting the death receptor apoptotic pathway and TNFSF10 and other folks in all probability activating the NFB pathway. TNFRSF14, TNFRSF4, TNF and TRADD. Of curiosity was TNFSRF14, which may interact with a few members of TRAFs to activate innate immune response in concordance with improved expression of several members of your TRAF household. Moreover, a group of of dipeptidylpeptidases the vast majority of that are serine proteases concerned in cleaving proline adjacent bonds, showed greater expression mainly at eight 24 hrs.
read this post here The effectively characterized DPPs impli cated in cytolysis are DPP1. involved in processing of GZMA, B and DPP4. a serine protease concerned in CD16 mediated cytolysis by NK cells. Cytokines, chemokines and their receptors A few chemokine ligands and receptors showed higher expression in resting cells very likely associated on the instant immune response of NK cells. A group of chemokine receptors and CX3CR1 showed an early improved expression just after IL2 stimulation. Later on a substantial variety of chemokines in addition to a number of chemokine recep tors. CCR5, CCR6 and CXCR6 showed elevated expression at eight 24 hours. The elevated expression of these genes is probably responsible for that proinflammatory response of NK cells, such as the recruitment of NK cells as well as other immune cells to web sites of irritation. A lot of interferons and interferon induced genes were upregulated. IFN inducible protein 16, IFN regulatory issue 6. 2, 7, IFNA16, 4, IFNAR1, two, IFN induced protein 35.
Macroscopically incomplete resection was thanks to an internal spot with huge tumor size. The 8 patients with R2 status right after surgical procedure obtained submit operative pal liative chemotherapy. Five also obtained radiotherapy that delivered a total dose of 50 Gy or thirty Gy before or just after the end on the initial line chemotherapy. Four with the eight individuals showed rapid dis ease progression with chemotherapy. Five patients did not undergo surgery and acquired only palliative chemotherapy. They showed progressive disease following 2, 5, five, 7 and 9 months, respectively. All 21 patients showed treatment failure, with median time to therapy failure 7. eight months. On the time from the last stick to up,19 patients were dead, all as a result of cancer. two were still alive at 138 and 167 months, respectively, of observe up. The 1st patient knowledgeable two other MPNSTs as well as second community recurrence, which was handled with sur gery and radiotherapy.
The two had localized grade 3 tu mors with R0 resection followed by chemotherapy early right after surgery. The chemotherapy regimen was ifosfamide and doxorubicin and was not associated selleckchem GDC-0068 with radiotherapy. Survival at 12, 18 and 24 months was 81%,47. 6% and 38%,respect ively. The median general survival for that whole cohort was 17 months. The median time for you to survival for sufferers with peripheral MPNSTs was 21. four months and for sufferers with axial MPNSTs, 12. 6 months. Univariate examination uncovered no association of variables examined and time for you to therapy failure or total survival. Improved tumor dimension at diagnosis was as sociated using a brief time for you to treatment failure and total survival. As in contrast with locally superior or metastatic dis ease, R0 and R1 status was related with lowered danger of death. Because of the little amount of patients, we could not determine aspects associ ated using the 2 amputation failures.
Discussion In our working experience managing NF1 with MPNSTs by chemotherapy, general survival was bad, by using a median time of 17 months and five yr survival of 14%. BMS387032 While a big difference in survival among sporadic and NF1 relevant MPNSTs continues to be a matter of debate, sev eral research showed the prognosis of patients with NF1 is bad. This bad final result may be explained by many points. For most of our patients, MPNSTs had a deep place, with significant dimensions and large histological grade,as previously shown. We reveal a reduced charge of comprehensive surgery in accordance to your localization and size of lesions at diagnosis. only 6 from the 21 patients had R0 resection, and regional control rate was reduced. Certainly, the majority of the MPNSTs have been internal as previously proven. In contrast, for sufferers with per ipheral MPNSTs, survival was much better, even though not signifi cantly, than with axial MPNSTs.
NF ?B is activated by phosphorylation of I?B through activation of MAPKs including ERK1 two, JNK and p38 subfamilies, and after that migrates to the nucleus and activates the expression of inflammatory cytokines and mediators. The activation of NF ?B in response to professional inflammatory stimuli for instance TNF as a result of phosphorylation of I?B success in the NF ?B Raw 264. 7 cell line was obtained from cell financial institution, Insti tute of Biochemistry and Cell Biology. Raw 264. seven cells have been cultured in DMEM with 10% fetal bovine serum, in an incubator at 37 C, 5% CO2 and 95% humidity. The cyctotoxic effects of WEL had been evaluated in absence or presence of LPS by MTT assay. WEL was dissolved in 10% dimethyl sulfoxide and additional right to culture media before the addition of LPS. The last concentration of DMSO under no circumstances exceeded 0. 1%. Measurement of NO levels The nitrite concentration within the culture medium was measured by a Griess reaction check.
Cells had been plated like a density of two ? 106 cells properly in 24 nicely culture plates and pretreated with or with no indicated concentrations of WEL or N nitro L arginine methyl ester for twelve h, then incubated with LPS. one hundred uM L Title, an inhibitor of NO, was applied like a constructive manage. Just after twenty h incubation, cells selleck chemical Serdemetan were washed three times to clear away non adherent cells. Then, one hundred ul of your Griess reagent was mixed with an equal volume of cell supernatant, the optical density at 540 nm was measured plus the concentration of nitrite was calculated in accordance to your standard curve created from acknowledged concentrations of sodium nitrite. Measurement of PGE2 ranges RAW 264. 7 macrophages have been subcultured in 24 well plates and pretreated with or without having indicated concen trations of WEL for twelve h or DX for 1 h, then incubated with LPS for twenty h.
The accumulated PGE2 inside the culture medium was measured working with ELISA Kit in accordance on the makers directions. 0. one uM DX was applied as being a favourable control. Measurement of TNF levels The effects of WEL within the manufacturing of TNF have been measured by ELISA. 2 ? 106 RAW 264. 7 cells had been seeded on 24 very well plate at a density selelck kinase inhibitor of 2 ? 106 per well for more than evening. Cells were pre incubated with WEL or DX for 1 h, then stimulated with one ug ml LPS for yet another 20 h. The cytokine concentrations have been calculated in accordance to your conventional curve working with recombinant cytokines in each and every ELISA kits. All measurements over were carried out in triplicate. Transient transfection and luciferase reporter assay NF ?B reporter constructs had been obtained from Clon tech Laboratories, Inc. For that reporter assay, cells have been seeded into 24 very well plates at a density of 5 ? 105 cells per very well in 500 ul of DMEM without having antibiotics and incubated overnight. The cells in each well have been transiently transfected with 200 ng of luciferase reporter construct and 50 ng of internal control plasmid of your pCMV B galactosidase reporter plasmid or empty expression vector pcDNA3 using li pofectamine TM 2000 reagent according to your manu facturers procedures.
Growth inhibition results of salirasib are p53 indepen dent as salirasib have an effect on in a comparable trend HepG2 and Hep3B cells. This really is additional sub stantiated from the lessen in p53 expression observed after 2 days of treatment in HepG2 cells. This factor could possibly be clinically appropriate, for the reason that most human HCC harbor defective p53 function, A treatment strongly based upon p53 activation could thus be much less effec tive in these tumors. Our results contrast using a prior report of enhanced p53 perform in colon cancer cells in response to salirasib, Having said that, p53 downregulation is compatible with ras inhibition, since K ras activation is regarded to induce p53 up regulation, This lack of p53 upregulation in our examine may be linked to the absence of ERK inhibition upon therapy.
Without a doubt, in HepG2 cells, ERK is often a important activator of Mdm2, that is responsible for p53 degradation, Complete Ras protein expression was diminished inside the 3 examined cell lines after two days of therapy, even though Ras mRNA amounts remained steady. Also, salirasib reduced the expression of active GTP bound Ras in HepG2 cells purchase VX-809 stimulated with EGF. These observations indicate an increase in ras protein degradation, which is consistent using the postulated mechanism of action of salirasib, involving the dislodgement of ras from your cell membrane followed by a cytosolic degradation, Sur prisingly, salirasib was not able to inhibit neither ERK nor Akt phosphorylation. To the contrary, it even tended to boost their phosphorylation levels, which might be because of a powerful inhibition of p70 and also to the consequent relief of the detrimental suggestions loop affecting ERK and Akt, Importantly, p70 phosphorylation was abrogated on treatment method in all cell lines when stimulated with EGF, which occurred devoid of concomitant inhibition of ERK or Akt, each of that are recognized to activate mTOR.
Furthermore, salirasib also effectively diminished MK-2461 p70 phos phorylation in all cell lines upon IGF2 stimulation, a condition where stimulation with the Akt mTOR axis is independent of ras activation, Indeed, no ras activa tion above baseline amounts was observed in HepG2 cells stimulated with IGF2, and IGF2 did not induce ERK phosphorylation in any with the examined cell lines. Alto gether, these information recommend that salirasib induced inhibi tion of mTOR in HCC cells occurs, no less than in aspect, independently of ras, and thus level to a direct inhibi tory effect about the mTOR complicated one, confirming earlier observations, Nevertheless, it should not be concluded the development inhibitory effect that is observed in HCC cell lines solely relies on mTOR inhibition, as other unex plored ras mediators may very well be impacted. Even though, each ras and mTOR inhibition taken separately could make clear the lower in cyclin A plus the raise in p27 amounts, it truly is really worth to note that these alterations parallel the down regulation of ras in HepG2 and Hep3B cells.
Nevertheless, our acquiring signifies that none from the AKT isofoms had been able to advertise mesenchymal properties nor increase cell mobility in nonmalignant MCF10A cells, im plicating a likely tumor repressing in lieu of tumor selling part as indicated in earlier reports, Furthermore, to exclude the probability that our obser vation is due to the truth that MCF10A cells are immorta lized, this discovery was even more substantiated through the use of non immortalized major normal human mammary epithelial cells isolated from 3 various gals. Just like the outcomes obtained applying the MCF10A cells, activation of Akt inhibited the expression of mesenchymal linked transcripts selleck chemical and lowered cell motility in HMECs from all 3 donors. These effects weren’t linked with any distinct Akt iso form, together with the exception that expression of E cad was marginally repressed in HMEC 2 overexpressing Akt3 too as in HMEC three expressing all 3 isoforms, Likewise, N cad was largely inhibited in HMEC one and 2, but activated in HMEC three.
We view outliers as staying in all probability ascribed to an induction of one more unreported adhesion molecule which can trig ger an epithelial phenotype in lieu of E cad activation or N cad repression. The variability could possibly be because of the undeniable fact that commercial main breast epithelia are rather hetero geneous compared to MCF10A cells. Nonetheless, migra tion was prominently inhibited by activated Akt signaling, Taken with each other, for selelck kinase inhibitor the 1st time by studying all three Akt isoforms, our data recommend that overly activated Akt signaling can lead to a noticeable reduction of mesenchymal related transcripts at the same time as a lower in cell motility and they are observed in non malignant breast epithelial cells such as not simply immor talized MCF10A but also primary breast epithelial cultures HMEC, E cad Activated Akt signaling hinders IGF I and TGFB induced EMT in an isoform independent method The Akt pathway axis has been reported to get modulated by distinct isoforms, Most practical scientific studies of Akt isoforms have already been carried out through gene unique xknockdown of unique Akt isoforms in genetically modified mice.
However, the latter is constrained by species conservation and possibly biased from the proven fact that tumor microenvironment within the mouse might not normally reflect the that in humans, The discrep ancy of information evolved through the two method may well be ascribed to ectopic expression versus knocking down endogenous Akt, On the other hand, knocking down specific Akt isoforms appears to be much less relevant than overexpression techniques given that human car cinomas usually display aberrant activation and amp lification in lieu of suppression of Akt signaling, To decipher how unique Akt isoforms influence IGF I mediated EMT, MCF10A cells were retrovirally transduced to express IGF IR that subsequently grew to become phosphorylated and activated, which in turn induces EMT in response to ligand stimulation.
However, our acquiring signifies that none with the AKT isofoms were in a position to promote mesenchymal properties nor enhance cell mobility in nonmalignant MCF10A cells, im plicating a likely tumor repressing in lieu of tumor promoting position as indicated in past reports, Moreover, to exclude the probability that our obser vation is because of the truth that MCF10A cells are immorta lized, this discovery was additional substantiated through the use of non immortalized main regular human mammary epithelial cells isolated from 3 diverse gals. Much like the outcomes obtained using the MCF10A cells, activation of Akt inhibited the expression of mesenchymal connected transcripts selleck inhibitor and diminished cell motility in HMECs from all 3 donors. These effects were not related with any unique Akt iso type, with the exception that expression of E cad was marginally repressed in HMEC 2 overexpressing Akt3 also as in HMEC three expressing all three isoforms, Likewise, N cad was largely inhibited in HMEC 1 and 2, but activated in HMEC 3.
We see outliers as staying most likely ascribed to an induction of an additional unreported adhesion molecule which can trig ger an epithelial phenotype in lieu of E cad activation or N cad repression. The variability may be because of the proven fact that industrial key breast epithelia are rather hetero geneous compared to MCF10A cells. Nevertheless, migra tion was prominently inhibited by activated Akt signaling, Taken together, for selleck chemicals tgf beta receptor inhibitor the very first time by learning all 3 Akt isoforms, our information recommend that overly activated Akt signaling can lead to a obvious reduction of mesenchymal associated transcripts as well as a decrease in cell motility and they are observed in non malignant breast epithelial cells which include not merely immor talized MCF10A but additionally primary breast epithelial cultures HMEC, E cad Activated Akt signaling hinders IGF I and TGFB induced EMT in an isoform independent manner The Akt pathway axis continues to be reported to get modulated by distinct isoforms, Most functional research of Akt isoforms happen to be carried out via gene precise xknockdown of precise Akt isoforms in genetically modified mice.
Nevertheless, the latter is restricted by species conservation and possibly biased by the proven fact that tumor microenvironment in the mouse might not normally reflect the that in humans, The discrep ancy of information evolved from the two system may well be ascribed to ectopic expression versus knocking down endogenous Akt, However, knocking down distinct Akt isoforms seems to be much less pertinent than overexpression techniques due to the fact human car cinomas often display aberrant activation and amp lification as opposed to suppression of Akt signaling, To decipher how diverse Akt isoforms influence IGF I mediated EMT, MCF10A cells were retrovirally transduced to express IGF IR that subsequently became phosphorylated and activated, which in flip induces EMT in response to ligand stimulation.
The information, even so, recommend that other genetic adjustments could influence the standing of pAKT, rendering it unregulatable by HSP27. For example, the reduction of PTEN, a common aberration in gliomas, results in elevated pAKT. It is less clear as to no matter whether PTEN standing influences the outcome of TMZ treatment, as you will find reviews that wildtype PTEN correlates with elevated survival during the drug treated sufferers and people demonstrating no benefit, Moreover, the outcome could possibly be influenced by MGMT promoter methylation standing. Sufferers acquiring tumors with PTEN favourable tumors with methylated MGMT had a survival benefit when handled with TMZ plus erlotinib and RT, Even more studies are wanted. The combined information working with established cell lines indi cate that blocking HSP27 is surely an productive treatment strategy, but is a lot more effective if SPARC expression is forced and promotes death signaling.
A serious query is whether key selleck inhibitor gliomas have forced or non forced SPARC expression. Two pri mary human glioma cell lines have been treated with control or HSP27 siRNA during the absence or presence of TMZ. Treatment method with HSP27 siRNA resulted in decreased col ony forming efficiency for each cell lines, Nonetheless, the results of HSP27 inhibition on signaling had been diverse concerning the cell lines, Inhibition of HSP27 in HF373 cells did not do away with SPARC suggesting a forced SPARC expression profile as observed to the H2 cells. In contrast on the H2 cells where substantial SPARC expression correlated with substantial pAKT, pAKT remained lower in the HF373 cells. As we had previously demonstrated that PTEN reconstitution could suppress SPARC induced activation of AKT, we considered the PTEN status for this cell line. No mutation has nonetheless been described for HF373, suggesting a wildtype status.
This suggests that wildtype PTEN sup presses SPARC induced pAKT in these cells. For HF2303 cells, inhibition of HSP27 only decreased SPARC by 50% and pAKT remained higher, BI-2536 also recommend ing a forced SPARC profile. Also, PTEN is mutant in HF2303. Hence, SPARC expression com bined with loss of PTEN was adequate to advertise ele vated pAKT. Consequently, the 2 cell lines had a forced SPARC expression profile, but the resultant impact on pAKT amounts differed, most likely resulting from variations inside the PTEN standing. As a consequence the loss of HSP27 promoted apop totic signaling in both cell lines. Nevertheless, the HF373 cells demonstrated greater autophagy, whereas the HF2303 cells didn’t. During the latter cells, autophagy was induced with all the AKT IV inhibitor. These observations are in agreement with observations that knockdown of AKT exercise increases autophagy, and apoptosis will not be the prevailing response, Thus, we propose that HSP27 inhibition alone will be most efficient in SPARC positive PTEN wildtype tumors, even though mixed inhibition of HSP27 and pAKT will very likely be warranted for tumors which can be SPARC positive PTEN null.
To serve as a control, we first validated the action of your personal LIP and LAP2 constructs on a C EBPb responsive promoter as shown in Figure 2A. C EBPb null mammary epithelial cells were transfected with either LIP, or LAP2 individually or together with a C EBP responsive, firefly luciferase construct and renilla luciferase construct as control. As expected, LAP2 expression led to a rise in C EBP responsive luciferase activity whilst LIP alone reduced promoter exercise, In combination with LAP2, LIP expression antagonized and decreased LAP2 induced promoter exercise and led to a lower in luci ferase activity. To check for IGF 1 induced, endogenous C EBPb exercise, MCF10A cells were transfected by using a C EBP responsive, luciferase construct before stimula tion with IGF 1. To maximize LIP expression for a sig nificant boost the LIP LAP ratio, cells had been stimulated for sixteen hrs with 39 nM IGF 1.
This led to an anticipated lower in C EBP responsive luciferase action as a result of antagonistic effects of elevated LIP expres sion, These information show that IGF 1R induced increases from the LIP LAP ratio are biologically active. Does IGF 1R and Insulin regulate LIP expression via the activation of order Blebbistatin the EGF receptor Due to the fact IGF 1R signaling is observed to cross talk with EGFR signaling, it had been needed to identify no matter if the IGF 1R induced expression of LIP was, in component, mediated by EGFR signaling. We for that reason investi gated no matter whether therapy of MCF10A and MCF7 cells with IGF one leads to phosphorylation of EGFR. As deter mined by Western blot analysis, neither IGF 1 nor insu lin stimulation led to a substantial increase in EGFR phosphorylation as assessed in whole cell protein extracts 10 minutes soon after addition of ligand.
Moreover, neither a 10? maximize in IGF 1 nor insulin activated the EGF receptor, However, immunoprecipitation followed by immunoblot examination did display a modest boost in phosphorylated EGFR immediately after 10 minutes of IGF 1 stimulation, As well as IGF one and insulin receptors, mammary epithelial cells may also express insulin IGF one hybrid receptors, Hybrid receptors have been detected in most tissues that express each insulin over at this website receptor and IGF one receptor. An IGF 1 concentration of two. six nM won’t activate the insulin receptor, but could probably result in the activation with the insulin IGF 1 hybrid recep tors. Information presented in Figure 3C supports this hypoth esis and suggests that IGF 1 signaling has led on the formation of insulin IGF 1 hybrid receptors.
Practical research with hybrid receptors show they behave additional like IGF 1 receptors as opposed to insulin receptors due to the fact they bind IGF one which has a much better affinity than insulin, As expected, we did not observe activation of your hybrid receptor with ten nM insulin, Despite the fact that the significance with the hybrid receptors in mammary epithelial cells in unclear, we hypothesize that the insulin IGF 1 hybrids may be far more abundant in MCF10A cells than otherwise anticipated and this hypothesis is supported by reviews that insulin and hybrid insulin IGF one receptors are critical regulators of breast cancer cells, All through this research, we’ll refer to your IGF 1R mediated induction in LIP for simplicity, but the reader must recognize that hybrid receptors can also be concerned in regulation of LIP LAP.

domain: biology


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Check these symptoms of COPD
If he is not a smoker he could still have it
Has he had a chest x-ray if not that is what I would look into. Also were any of the meds inhaled steriods (like flovent) and was he prescribed any other type of inhaler? Has he been exposed to anything different starting in January?
I hope you get your answers,
PS As any part of the homeopathic included a good chiropractor?
He could have allergies even after testing,
JANUARY, THAT'S THE BEGINNING OF TREE ALLERGIES..
I also have a horrible cough that leaves me exhausted.. Chest x-rays, mri's, cat scans, allergy tests only show grass, weeds as allergy related. He could have post-nasal drip causing him to cough. Elevate his bed (pillows under mattress) portion. He could also have "reflux" and should try otc prevacid for acid reflux..stay away from spicy foods.Try using the neti pot (which has done wonders for me vs. medication) which can clear up some of the mucus that is dripping down the back of his throat. Mucinex 12hr.release tablets can help thinning out the mucus also. Dairy products will only make more mucus. Check inside home for "mold" which can be a cause of coughing also. I'm hoping this information will help. Hope the best for you!!!

domain: health


Repair crews work on the FBI's aging headquarters building in downtown Washington. The Congress and Biden administration plan to move the headquarters to one of three possible suburban sites, two of which are in Maryland. (Photo: Emily Hahn)
WASHINGTON (April 29, 2022)—The final selection process for a new location for the headquarters of the Federal Bureau of Investigation is back on track after years of delays.
The FBI headquarters has been located in the J. Edgar Hoover Building in the nation's capital since 1974, but the building is deteriorating. Trippy.com in 2012 named the agency's home the world's "ugliest" building and in 2005 architect Arthur Cotton Moore said the building "creates a void along Pennsylvania Avenue."
Talks between the FBI and the General Services Administration began on a new site for the headquarters during the Bush administration. But more focused efforts got underway during the Obama administration to move the headquarters out of the District of Columbia to what eventually was narrowed down to three potential sites in the suburbs, two in Maryland and one in northern Virginia.
The Biden administration has a Sept. 11 deadline for selecting one of the three proposed sites. The new headquarters would house an estimated 7,500 employees.
Sen. Chris Van Hollen, D-Maryland, has been one of the leading lawmakers in getting the headquarters relocated to a new suburban location. However, the Trump administration previously had blocked efforts to move the headquarters out of Washington.
"We were extremely disappointed when the Trump administration derailed this process for four years," Van Hollen said in an interview with Capital News Service. "We could have been moving forward to meet the security needs of the FBI... on the plan to consolidate the headquarters in one of these locations."
Van Hollen, who is the chairman of the Senate Appropriations Committee's financial services and general government subcommittee, worked with his Republican counterparts to put language in a recent bipartisan catch-all spending bill passed by Congress to resume the relocation process for the FBI headquarters.
"I think that senators on both sides of the aisle share a view that we need to provide the FBI with a secure home, and one that allows them to consolidate its operations," Van Hollen said.
President Joe Biden's proposed fiscal 2023 budget also includes language providing for a consolidated FBI headquarters in a new location outside the District of Columbia.
"Over the next year," the president's budget states, "GSA and FBI will finalize an updated program of requirements for a secure suburban campus, including the final number of personnel, to inform a 2024 Budget request for the new facility. GSA will also begin initial steps to acquire, if necessary, the site for the new suburban location."
The three potential headquarters sites are in Greenbelt and Landover, Maryland and Springfield, Virginia.
"I think this is the right move for the FBI and the country," Van Hollen said. "I think that either of the places in Prince George's County will put them in a position to consolidate their operations in a secure environment and carry out their duties on behalf of the country."
Van Hollen said the two Maryland locations both have access to public transportation, including Metro. Van Hollen also said the greater economic activity would benefit surrounding areas.
Rep. Gerry Connolly, D-Virginia, backs the Springfield location.
"The Springfield, Virginia, site is the best option for a new consolidated FBI headquarters and if evaluated on merit would be the clear favorite for this major procurement," Connolly said in a statement to Capital News Service.
"Northern Virginia is home to the FBI Academy at Marine Corps Base Quantico and the Central Records Complex in Winchester," Connolly said. "Additionally, the region boasts the highest concentration of suburban-located Intelligence Community (IC) agency headquarters in the country. Proximity to FBI assets and longstanding IC partners is a key competitive advantage for the site."
Like the Maryland locations, the Springfield site is near public transportation.
"The Springfield site is also at the transportation nexus of Interstate 495 and Interstate 95, the Washington Metro system (and) the Virginia Railway Express, which is slated to add considerable additional capacity in the coming years, and other transit services," Connolly said.
But not all are on board with the proposed idea of a move, including the FBI's current leadership. The agency wants to keep its headquarters in Washington and even has suggested the idea of having a separate cyber campus.
"The FBI can more effectively serve the American people from a headquarters located downtown," the FBI said in a statement to CNS. "Our mission would be enhanced by a consolidated suburban cyber and technology campus within the national capital region to serve as a command center for cyber operations, consolidate the FBI's existing cyber and technology footprint, and accommodate future growth."
The FBI Agents Association has joined the FBI in voicing its support for keeping the headquarters in Washington.
"We believe what is best for the mission is for FBI headquarters to remain in Washington, D.C., in close proximity to the Department of Justice and our DOJ counterparts," association President Brian O'Hare said in a statement to CNS.
But local lawmakers disagree.
"I don't think that the FBI leadership should be decapitated from the men and women who make up the FBI," Van Hollen said. "That's why the legislation says the headquarters needs to move."
Like Van Hollen, Connolly dismissed the FBI's desire to stay in Washington.
"It's been known for quite some time that Director (Christopher) Wray doesn't want to move," Connolly said. "One director does not get to make this kind of decision, nor does he get to circumvent the law."
Van Hollen said the Central Intelligence Agency headquarters located in suburban Langley, Virginia could be an example of what a suburban campus for a new FBI headquarters would look like. The National Security Agency headquarters in Fort George G. Meade, Maryland, is another good model, he said.
"Those are important models that provide both the opportunity to consolidate operations and also meet the security requirements," the senator said.
Biden's proposed budget does direct the GSA and FBI to find a federally-owned site in the District of Columbia that would be a satellite office for between 750 and 1,000 FBI employees who would be needed for daily contacts with the Justice Department, other parts of the executive branch and Congress.
The next step of the process is a GSA briefing by June 13 on the three sites to relevant congressional committees.
"The U.S. General Services is committed to continuing to support the Federal Bureau of Investigation (FBI) to address the FBI's mission needs, to execute in compliance with congressional direction, and to achieve the best value for the American people," according to a GSA statement to CNS.
"In our conversations with the Biden administration, we have urged them to reach a final decision about the actual location," Van Hollen said. "I hope that they will stay on track but what I am confident about is that this process is going to move forward."


text:
~ I have a thing for bookmarks and ornaments!
~ Reading is my drug! If you know me you know that. It's an escape into another life, into another reality.
~ I wear only Victoria Secret bras and undies. They are super cute and amazingly comfy! I have am obsession with undies.
~ I'm nerdy in some ways.
~ Blue is my absolute favorite color, followed by pink of course
~ I'm a girly girl but I was always a tomboy growing up. I love guy stuff! I'm not your typical average girl.
~ I could eat Pizza everyday... Along with Mac n cheese! Yummm
~ Penguins are my fave Animal! Obsessed! Eeeeek!
~ I love any cute stuffed animal
~ Writing is a release for me. It's a way for me to release my thoughts, feelings, anger, depression etc...
~ I love taking pictures. Lucas hates it! Lol. I would love to go back into photography. Maybe take a class at Owens.
~ I lOVE anything superheroes. Movies, clothes, accessories anything! Teenage Mutant Ninja Turtles and Batman are by far my favorites! Always have been!
~ HUGE animal lover right here!
~ I like eating healthy.
~ But I love candy!
~ I hate pop. I don't let Lucas drink it, but on a rare occasion. I'd rather not have pop even in my house.
~ I'm an Irish girl, which means I'm a beer drinking girl!
~ Lucas is my life! I tend to be obsessed with him. Lol. But he's my one and only. Followed by Lucas is princess Harlie. She's my little fatty fat. Can't imagine not having her. I got her a few weeks before I found out I was pregnant.
~ I battle depression on a daily basis. Some days are worse than others, but most days I hide it pretty well.
~ my phone is my bad addiction.
~ I love shooting my bow!! It's so calming. One day I would love to go hunting.
~ I'm not a super adventurous person. I have a hard time with change and going out of my comfort zone but it doesn't stop me from trying new things. I wish I was more adventurous but when it comes down to it I'm a worrier and chicken shit lol.
~ country music is the shit!!
~ My shows...for now that is lol are. Criminal Minds, Chicago Fire, Decious Maids, Duck Dynasty, and Castle. Thank goodness for DVR because sometimes it takes me weeks to watch them!
~ Love sports! Football (Lions), baseball (tigers) hockey (red wings) basketball (celtics)
~ sports I participate in- baseball, basketball, football, soccer, tennis, basketball, golf, batting cages, shooting guns, shooting my bow, fishing, bowling, tennis, running, I'm down with it all!
~ I love playing video games!!! Especially fighting ones. Or shooting. I love mortal kombat! all the batman games! call of duty. I'll play any of them once. Racing games also!
~ My eye sight sucks. Supposed to wear glasses but I don't.
~ I'm a terrible passenger. Car or plane!
~ I've never really traveled anywhere. Been on one vacation and that was to Cancun. But I would love to do more. I want to just drive and not have it planned and make random stops and see where I end up and what I see. To me that would be fun!
~ A trip to the beach seems like a vacation. Laying on a blanket in the sun with a book. Heaven!
~ Ever since my dad can remember I have been obsessed with visiting the Rocky Mountains. Only in Colorado though. I have no clue why! Lol
~ I want to own a farm with tons and tons of animals!
~ I'm a dreamer. Bad... And I day dream.. I drift away and dream. All. The. Time. Lol
~. I hate having all eyes on me. Which is why I didn't want a big wedding.
~ I can't dance, nor have any attention learning.
~ I HATE bugs!!!!!
~ I'm a good friend, i would do anything for my friends, I just never seem to get that in return.
~ I want to take drum lessons.
~ I love to cook! I love trying new recipes. Every week I am trying a new one.
~ I really would love to have another baby. I wanted 3 and I have 1. But I will settle for 2. I think it will kill me later if I only have 1. It's not something I can get passed, that feeling, that longing for another child. Lucas wasn't planned. I don't eant to plan my next either. It's scarier planning one than not planning it!

domain: hobby


text:
Pittsburgh took the opening
kickoff and moved down the field on a 71-yard scoring drive, with
Ben Roethlisberger completing a 38-yard
pass to Hines Ward and a 21-yard strike to Heath Miller, putting
the ball at the Arizona 1-yard line. On third down, Roethlisberger
appeared to score on a quarterback scramble, but it was overruled
by a replay challenge, which determined that he was down before the
ball crossed the goal line. Rather than make another attempt at a
touchdown, Pittsburgh settled for a Jeff Reed 18-yard field goal
to take the 3–0 lead. It was the second straight year a team
took the opening kickoff down for a score as the Giants did the same
in Super Bowl XLII (the last time before that was the Falcons in
Super Bowl XXXIII). The Steelers quickly forced an Arizona punt and
then drove back down the field for what would turn into more points.
On the first play of their drive, Roethlisberger completed a 25-yard
pass to Santonio Holmes. Following three more completions to Miller
for 26 yards and another one to Holmes for 7, reserve running back
Gary Russell went into the end zone for a 1-yard touchdown run to
make the score 10–0 on the second play of the second quarter.
They became the first team to score on its first two drives since
the Denver Broncos in Super Bowl XXXII. On defense, Pittsburgh held
Arizona to just one drive and one first down in the first quarter,
while gaining 135 yards.
The Cardinals got going for the first time midway through the second
quarter as a 45-yard completion from Kurt Warner to Anquan Boldin
moved the ball to the Steelers' 1-yard line. On the next play, Warner
nearly fell over after taking the snap, but he regained his balance
and threw a 1-yard touchdown pass to tight end Ben Patrick. After
an exchange of punts, Roethlisberger threw a pass that was tipped
at the line of scrimmage and intercepted by linebacker Karlos Dansby
at the Steelers 34-yard line with 2:46 left in the half. Seven plays
later, the Cardinals drove to a first down on the Pittsburgh 1-yard
line. But with 18 seconds left, Warner's pass was intercepted in
the end zone by linebacker James Harrison, who then took off down
the sideline for the longest play in Super Bowl history, a 100-yard
return for a touchdown, increasing the Steelers' lead to 17–7
at halftime. A booth review was called to verify that Harrison had
broken the plane, as he was tackled at the goal line, and the ruling
After forcing a punt, the Steelers started off the third quarter
with another long scoring drive. Aided by three personal foul penalties
against Arizona, they moved the ball 79 yards in 14 plays and took
8:39 off the clock. However, they were unable to get into the end
zone, despite two first downs inside the Cardinals 10 (a penalty
against Arizona on a Steelers field goal attempt gave them another
chance), and they had to settle for another Reed field goal to give
them a 13-point lead, 20–7. After a few more punts, Warner
led the Cardinals down the field on an eight-play, 87-yard scoring
drive that took 3:57 off the clock, utilizing a no huddle offense.
With 7:33 left in the game, Warner threw a high floating pass to
Larry Fitzgerald, who made a leaping catch through tight coverage
by Ike Taylor for a touchdown, making the score 20–14.
Later on, Ben Graham's 34-yard punt pinned the Steelers back at
their own 1-yard line. Two plays later on third down and 10, Roethlisberger
threw a 20-yard pass to Holmes, but center Justin Hartwig was called
for holding in the end zone, which not only nullified the catch,
but gave the Cardinals a safety, raising the score to 20–16.
Taking over on their own 36 after the free kick, Arizona took two
plays to score, as Warner threw a pass to Fitzgerald on a post route.
Fitzgerald caught the ball without breaking stride and took off down
the middle of the field past the Steelers secondary for a 63-yard
touchdown reception, giving Arizona their first lead of the game,
The final play of the gamePittsburgh got the ball back on their own
22-yard line with 2:37 left in the game and two timeouts remaining.
On their first play, a holding penalty pushed them back 10 yards.
Roethlisberger then completed two passes to Holmes for 27 yards.
After an 11-yard reception by Nate Washington and a 4-yard run
by Roethlisberger, he completed a 40-yard pass to Holmes at the
Cardinals 6-yard line. Two plays later, Holmes caught a pass in
the corner of the end zone and managed to land his toes down right
before falling out of bounds for a touchdown. After a booth review,
the touchdown pass stood. Reed's ensuing extra point made the score
27–23 with 35 seconds remaining. Following the ensuing kickoff,
Warner completed a 20-yard pass to Fitzgerald and a 13-yarder to
J. J. Arrington, moving the ball to the Steelers 44. With 18 seconds
left, Warner prepared to attempt a Hail Mary pass, but linebacker
LaMarr Woodley forced a fumble while sacking Warner, which defensive
end Brett Keisel recovered, giving the ball back to Pittsburgh
with five seconds left. Roethlisberger took a knee, sealing the
Steelers' NFL record sixth Super Bowl title.
Super Bowl XLIII Statistics
PIT – Jeff Reed 18 yard field goal. PIT 3–0
PIT – Gary Russell 1 yard run. PIT
ARI – Ben Patrick 1 yard pass from Kurt Warner. PIT 10–7
PIT – James Harrison 100 yard interception return. PIT 17–7
PIT – Jeff Reed 21 yard field goal. PIT 20–7
ARI – Larry Fitzgerald 1 yard pass from Kurt Warner. PIT
ARI – Holding penalty on Justin Hartwig in end zone for safety.
ARI – Larry Fitzgerald 64 yard pass from Kurt Warner. ARI
PIT – Santonio Holmes 6 yard pass from Ben Roethlisberger.
Pittsburgh Steelers - 27
Arizona Cardinals - 23
Super Bowl XLIII MVP Santonio Holmes Pittsburgh
Santonio Holmes, who caught nine passes for 131 yards and a touchdown,
including four receptions for 71 yards on the final game-winning
named Super Bowl MVP. He became the sixth wide receiver to win the
award, and also was the third Pittsburgh receiver to win the award,
following Lynn Swann and Hines Ward.

domain: sports


text:
Jay Varady making impact in first season as Coyotes assistant coach
The Arizona Coyotes didn't have to look far to fill a position on their bench among the coaching staff, officially adding Jay Varady just before this season started.
Varady was already an extension, in some ways, of the staff. He'd coached the Coyotes' American Hockey League team, the Tucson Roadrunners, to a 70-45-11 record from 2018 to 2020, and after sports came to an abrupt end with the arrival of COVID-19, came up to help the Coyotes in the bubble environment for last year's NHL postseason.
His familiarity with Coyotes prospects who've played for and currently play in Tucson was a plus as the team went into this year's training camp, and before it had ended, Varady earned a full-time position the NHL after two decades of coaching.
"You've got all these extra players around, so you really need an extra coach, almost, to keep those guys in shape outside the staff. So in the bubble, that was mostly my main role, which allowed me to spend more time with Rick Tocchet's staff," Varady said. "As we were approaching (this) season, we didn't really know if the American (Hockey) League was going to go. It was just a transition into a season that was going to be jam-packed.
"I was excited about the opportunity, and I've loved every second of it."
Coyotes head coach Rick Tocchet said having Varady this season has been a great resource.
"I really enjoy working with him, he's a very passionate guy about the game, and he wants to teach and he also wants to learn, too," Tocchet said. "He's always pushing the envelope and thinking outside the box."
The relationship Varady has with players like Michael Bunting, Lane Pederson, Adin Hill, and the taxi squad players that played for him in Tucson the past couple of years is important for Tocchet, who calls Varady a "conduit."
Varady has run a lot of taxi squad practices, the small-group sessions that typically take place after the main team gets their on-ice work done.
"He can talk to the guys. The taxi squad guys, he keeps them motivated," Tocchet said. "Helps me out because I've got a lot of stuff going on. He keeps those guys motivated and explains things to them, too."
Varady's time in Tucson allowed him to forge a good working rapport with the Coyotes coaches, and now that he's in Glendale, the organization's top player development people are only a few steps away at Gila River Arena.
Varady credited Tocchet for being very inclusive with the Tucson coaches and player development staff, so that everyone is on the same page with regard to the plan for how to bring those players in the minors along.
"I think that is the big thing, just the continuity of the whole group," Varady said. "Everything that's player-facing, from the development team to the American league team to the NHL coaching staff is really one plan and one mission, and I think that helps players here in Arizona."


January 19, 2010
A personal perspective on fox and coyote penning
November 19, 2009
The Humane Society of the United States commends the Florida Fish and Wildlife Conservation Commission for arresting 12 people for illegal activity associated with fox pens.
May 13, 2009
The HSUS documented apparent violations of state regulations at nearly a dozen wildlife pens, or controlled hunting preserves, in nine counties in North Carolina.
April 8, 2009
The HSUS applauded N.C. Rep. Harrell for introducing a bill to prohibit fox and coyote controlled hunting preserves.
July 10, 2008
Overstock.com's CEO Patrick Byrne adopts a fur-free policy for his company.


text:
THE SOLO MARIMBA COMMISSIONS VOLUME 3
by Payton MacDonald
Works by Akshaya Avril Tucker, Aakash Mittal, Don Freund, Peter Jarvis, Gene Pritsker & Travis Salim.
Available for Streaming on
by Saili Oak, Salastina Music Society
Works by Dr. Ashwini Bhide Deshpande, arranged for string quartet by Akshaya Avril Tucker, Reena Esmail, Shane Cook, Kara Ford & Anton Ariki Sten.
Download on www.tororecords.com
Available for streaming on
Pallavi, Meditation on Care for Solo Violin (2019) performed by Johnny Gandelsman
ASCAP Morton Gould Young Composer Award finalist 2020.
Three Songs for Hindustani Vocalist, Soprano, and Sinfonietta (2018) - performed by Saili Oak, Suzanne Lis, and prismatx ensemble.
Received an ASCAP Morton Gould Young Composer Award in 2019.
Breathing Sunlight, for Violin and Cello (2017)
Enough, to Unfold for Pierrot Ensemble (2018), choreographed and performed by Akshaya Avril Tucker and Namita Devadas.
I also perform as a cellist and dancer. Click the links to listen and watch.

domain: library


text:
A Diaper Machine is an innovative solution for superior quality diaper manufacturing and is available in fully automatic and semi-automatic configurations. It has a leading design with quality control, PLC control, and a smooth manufacturing process, designed according to the highest universal standards.
It is a high-performance machine suitable for diaper manufacturing units with varying capacities. The customization allows one-time investment as you invest in only the valuable features.
The Diaper Machine emphasizes higher productivity with a user-friendly interface and environment-friendly manufacturing.
With scientific development, customer-centered, and a productivity-oriented approach, we are one of the leading brands in manufacturing premium quality Diaper Machines.
We aim to apply artificial intelligence technology and higher useability of our equipment by integrating automation, low energy consumption, and customization for the better interest of our customers. Our main goal is to give you value for money and the best performance through constant research and improvement.
This article will help you to obtain the most out of your investment by getting the best performance. It contains all the information regarding the features, variety, maintenance principles, and working mechanism of the Diaper Machine to enjoy a smooth manufacturing experience. So let’s get started.
What is a Diaper Machine?
A Diaper Machine is an efficient solution for making high-quality diapers at a considerable speed, maintaining the quality. It incorporates advanced technology that enables you to customize it to add features and functionality according to the requirements of your application.
Along with reduced material waste, it offers higher speed and superior product quality with a user-friendly interface and easy-to-operate configuration.
Being the first choice for diaper manufacturing units worldwide, it meets the advanced challenges and allows higher performance with controlled energy consumption, thus making it affordable and convenient.
The Diaper Machine has a solid and sturdy stainless steel construction with maximum resistance against corroding agents, making it reliable for many years. Adding to the overall performance of your application, it helps you create various types of diapers with the highest quality control. It comes in multiple models, sizes, shapes, and capacities so that you can make a one-time investment.
What are the Basic Features of a Diaper Machine?
The essential features of a Diaper Machine are as follows:
- The Diaper Machine has a PLC control system and a humanized design making it easier to use.
- The transmission system is highly stable, allowing smooth manufacturing of different types of diapers with considerable speed.
- When the machine runs out of material, it automatically stops, thus making it more user-friendly and saving energy consumption.
- It deals with gentle mounting and stacking as well.
- The automatic shutdown is beneficial because it prevents the overloading of the machine.
- The two-level design enables it to occupy considerably low floor space, thus making it a compact assembly.
- It is available in various configurations according to your needs. The configuration decides the core design, specified commercial hardware, infrasonic bonding process, and adhesives.
- Bonding is glue-free, which adds to the affordability of the machine as it reduces the cost.
- The machine complies with global safety and electrical compliance and follows the rules and regulations Of CE/NFPA/OSHA.
- The ergonomic design enables the users to have a safe experience, and it has a sliding safeguard that allows total and easy access to the process units.
What are the Optional Features of a Diaper Machine?
The optional features add to the performance of your Diaper Machine and, therefore, you should consider these before purchasing it.
- Replacement of manual roll material loading and unloading with a Patented Automatic Roll Loading System (ARLS).
- Another benefit is that you can obtain faster product changeout with Advanced Recipe Manager (ARM).
- The remote control is available for remote and efficient operation.
- Customer-specific production data collection is also available.

domain: design


text:
Correct qualification and material selection is even more important when equipment is exposed to H2S-contaminated or other sour gas atmospheres.
These environments are both highly toxic and corrosive.
Many materials and products are susceptible to hydrogen embrittlement and stress corrosion cracking. The risk of catastrophic failures significantly increases.
C-FER provides clients with a wide range of sour service testing capabilities that help to understand and minimize various failure...

domain: chemistry


text:
My name is Camilla Lundsten and I run Camilla Lundsten Studio. Since 1997 I have worked with brand development and Creative Direction for global brands and local specialists in diverse markets
– food, fashion, beauty, interior, furniture, objects and toys.
Brands must communicate by having a dialogue with their consu-mers, to make them want to listen and care. Design is a dialogue and must have both conceptual and visual identity. First, a brand needs to find its strategic path and create solid platforms, so that every step that follows leads in a planned direction.
CREATIVE DIRECTION BY CLS OFFERS:
• Creating and directing strategy.
View selected Camilla Lundsten Studio projects under Clients


text:
A marine landslide in 2017 made a beach disappear. Ever since, a team has analyzed the site and recorded changes using PIX4Dmapper.
Do you want to get more out of your crop? Identify problems early? Optimize treatment plans? Do you know how to do it with drones?
Culverts are crucial for effective drainage around urban structures - but how can you inspect and maintain them if they’re underground?

domain: geography


Supreme Court weighs jurisdiction of juvenile gun case
The Indiana Supreme Court heard arguments about the jurisdiction of juvenile charges for dangerous possession of a firearm. (Courtesy IN.gov)
The Indiana Supreme Court heard arguments Thursday in the case of a young boy who admitted to dangerous possession of a firearm shortly before the jurisdiction of such offenses was upended by the same court.
“It’s not whether you can ever prosecute it, it’s where you can,” Chief Justice Loretta Rush said.
The 14-year-old defendant, known as M.H. in filings, was arrested in June 2019 in Elkhart County and admitted to delinquent acts, including dangerous possession of a firearm.
But in 2020, the Indiana Supreme Court ruled that juvenile courts couldn’t oversee those offenses because they had no adult equivalent. At that point, juvenile courts across the state had ruled upon dangerous firearm possession cases for more than two decades.
Spurred by that decision, the General Assembly inserted language granting jurisdiction to juvenile courts in 2021. However, the language wasn’t retroactive to previous cases, including the case of M.H., who had already received judgment on his case.
M.H.’s defense challenged his Elkhart County judgment, which the juvenile court denied, citing the new law. But the Indiana Court of Appeals reversed that decision, saying the court lacked jurisdiction at the time of its judgment.
“Both the Indiana and federal constitutions have a provision that… forbids the states to enact any law which imposes a punishment for an act which was not punishable at the time it was committed,” Mark Altenhof, M.H.’s defense attorney, said. “That’s exactly what happened here.”
Altenhof, under questioning, admitted the act was punishable under law, but clarified that no court had jurisdiction over it.
“The law applied to him, the problem was where to apply it,” Justice Mark Massa said.
Justice Christopher Goff and other justices expressed concern about granting M.H. relief when so many other juveniles had similar judgments against them
“I’m more concerned about an insurmountable gap in future cases, if your client were to get relief,” Goff said.
“We are stuck with the laws as they are written, not as we wish them to be,” Altenhof said. “I think that the more unfair results would be to have juveniles stuck with these adjudications where the juvenile court didn’t have subject matter jurisdiction in the first place.”
The state, represented by Supervising Deputy Attorney General Ellen Meilaender, argued the court didn’t need to overturn the 25 years of juvenile court rulings since juvenile cases didn’t have the same collateral consequences as adult cases and, frequently, were expunged when someone turned 18.
“If the court were inclined to do so, I think you could justify a rule that said there’s no retroactive application to juvenile adjudications because of their unique nature and that they don’t have the same ongoing effects that continue on in a person’s life (since) they are confidential,” Meilaender said. “But the state is not asking the court to do that, the state believes that (previous rulings suffice).”
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