What does the transition towards lowcarbon building stock mean for Europe?
European building stock is responsible for around a third of CO2 emissions, and the EU has set a target of 20 % reduction by 2020 and 60 % by 2030, from a 1990 baseline. Meeting these targets means focusing on decarbonising the power supply and making concerted efforts to reduce energy demand through retrofits of existing stock.
Post date: 14 Nov 2019
SolarPower Europe and The Solar Trade Association are bringing together first line experts on the O&M and Asset Management conference. This event will drive the discussion on enhancing excellence and transparency, as well as new digital solutions that the industry, the financial community and all the relevant stakeholders can benefit from. Leading companies that represent a significant share of Europe’s solar PV portfolio are already committed to this process.
Post date: 13 Nov 2019
This joint workshop organised by REHVA and the European Building Automation and Controls Association will present the wide spectrum of tools and technologies supported by BACS to improve and optimize HVAC systems’ performance and make it transparent to Building Owners/Operators.
Post date: 7 Nov 2019
Concept du salon
Post date: 6 Nov 2019
Programme Building Green Copenhagen 2019
Knowledge-sharing platform and product exhibition
Building Green is a knowledge-sharing platform for the brightest actors in the field. It is a meeting point for turnkey products from a wide range of manufacturers in the construction and energy sectors. It constitutes a unique opportunity to showcase energy solutions targeting the professionals in the industry.
Post date: 25 Okt 2019
Smart grid is triggering the transformation of traditional electricity consumers into electricity prosumers. This paper reports a case study of transforming an existing residential cluster in Sweden into electricity prosumers.
The main energy concepts include:
Post date: 24 Okt 2019
The EU-funded EU-Korea Climate Action project supports Climate Partnerships between Korean and European non-state entities who commit to implement climate actions in priority areas such as renewable energy, energy efficiency in buildings, sustainable mobility, circular economy and best low-carbon business practices.
Post date: 21 Okt 2019
The Spara and bevara* database contains bibliographic records on articles and books dealing with energy efficiency in historic buildings. You will also find full-text documents and links to electronic resources.
*Spara och bevara is Swedish for "Save and preserve" as in save energy and preserve historic buildings.
Link to the database: http://eprints.sparaochbevara.se/
Post date: 17 Okt 2019
Post date: 11 Okt 2019
Hardi, can you tell us a bit more about what you do and what Rannaliiva is and does?
Post date: 4 Okt 2019


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Mirena® IUD MDL has been filed in New York.
Mirena® IUD Multidistrict Litigation Filed in the Southern District of New York. Mirena® IUD multidistrict litigation has recently been filed against Bayer Healthcare Pharmaceuticals, Inc., (“Bayer”) the manufacturer of the device, in the Southern District of New York. Many plaintiffs in these cases experience numerous complications, leading to serious injuries and sometimes even death. Additional cases are also being filed nationwide. Specifically, 75 cases had been filed In New Jersey state court, as well as 50 more being filed in federal courts across the country. These numbers are expected to increase, as over 15 million women currently use the Mirena® IUD across the globe. In general, these lawsuits allege that the IUD is flawed in its design, leading to an unreasonable risk of injury to its users. Further, plaintiffs claim that Bayer failed to properly warn consumers of the risks associated with the Mirena® IUD.
The Mirena® IUD, originally released in 2000, is a small piece of plastic shaped like a “T” with a small filament attached thereto that contains Levonorgestrel, a time-released hormone used to prevent pregnancy. When placed in the uterus by a healthcare professional, the device is intended to remain there for upwards of five years. However, many women have experienced the IUD migrate within the uterus, sometimes causing it to perforate, or even worse, become embedded within the uterine wall. Removal of an embedded device requires a surgical procedure to correct, leading to pain and potential future complications.
According to the U.S. Food and Drug Administration (“FDA”), the most serious side effects can include;
- Perforation of the uterine wall: a potential complication leading to injury of the viscera such as the bowels and uterus. In addition, uterine perforation and its associated complications can lead to severe bleeding and infection, also known as sepsis.
- Embedment of the device in the uterine wall: the device can embed itself in the wall of the uterus and cause injury to other organs as a result.
- Ectopic Pregnancy: also known as a tubal pregnancy, this occurs when a fetus embeds itself in the fallopian tube.
- Group A streptococcal sepsis: this is a dangerous bacterium that can lead to complications ranging from minor sickness to even death.
- Pelvic inflammatory disease (PID): a common infection of a woman’s reproductive organs that if left untreated, can cause damage to the fallopian tubes, ectopic pregnancy, infertility, and chronic pain and complications.
In 2009, Bayer reprimanded by the Department of Health and Human Services’ Division of Drug Marketing, Advertising, and Communications for exaggerating the benefits of the product and downplaying the risks. Specifically, Bayer had marketed the Mirena® IUD as a way in which busy moms simplify their life styles. Specifically, Bayer held “Mirena Parties,” creating a direct marketing program that used a series of “house parties” to introduce women to the product. Scripted phrases such a “look and feel great” and you’ll be “more likely to be more intimate” were emphasized. It was on this basis that the FDA warned the manufacturer against implying that use of the product would lead to a more satisfied life. Unfortunately, what was downplayed at these “parties” were the potential for serious side effects. As a result, the FDA sited Bayer over the scripted statement that the IUD “has no daily, weekly, or monthly routines to comply with,” which the agency asserted was false.
Are you experiencing complications from you Mirena® IUD?
Your first stop should be to see your doctor immediately. Then, contact our legal team at 1-800-YOURLAWYER (1-800-968-7529) today to learn more about your legal rights and interests. Don’t let big pharmaceutical companies intimidate you. We have the legal skills and experience our disposal to fight for everything you may be entitled to receive. Contact Parker Waichman LLP today to learn more about how we can make a difference for you.
New York | Brooklyn | Queens | Long Island | New Jersey | Florida
Call us at: 1-800-YOURLAWYER (800-968-7529) | Schedule your free consultation

domain: medical


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The Sarbanes-Oxley Act
Whether Sarbanes-Oxley has done anything to curb fraud is subject to debate. The Act has, in a larger part, enhanced efforts to curb fraud as the measures established in the Act have led to improved corporate governance practices and better accountability. According to Upadhyay & Triana (2021), research suggests that Sarbanes-Oxley (SOX) has effectively reduced misrepresentation of financial information, thus reducing fraud. Notably, this is so because the Act raises awareness of financial reporting issues and corporate governance. However, Upadhyay & Triana (2021) note that it is hard to quantify the gains in reducing fraud through the Sarbanes-Oxley Act. The Act has various impacts on U.S. companies competing globally. First, the companies have to incur compliance costs of implementing and maintaining SOX through internal controls installation, reporting obligations, and independent audits. Second, U.S. companies competing globally gain an advantage over foreign competitors in investor confidence. Companies adhering to the Act have greater investor confidence than those that do not comply.
Upadhyay, A., & Triana, M. D. C. (2021). Drivers of diversity on boards: The impact of the Sarbanes‐Oxley act. Human Resource Management, 60(4), 517-534.
We’ll write everything from scratch
The Sarbanes-Oxley Act is a product of a series of scandals that took place around the turn of the millennium.
Has Sarbanes-Oxley really done anything to curb fraud?
How does the Sarbanes-Oxley Act affect U.S. companies as they compete globally?

domain: finance


text:
Sir Michael, who was then in his 30s, went on to tell her that she was an “in quotes, a serious actress” and asked if her “equipment” undermined her credibility. “Your figure”, he added when questioned by the actress what he meant. “Serious actresses can’t have big bosoms, is that what you mean?” she replied, outraged. He also quoted a review that described her as “projecting sluttish eroticism” during the encounter, which Dame Helen later described as “enraging” and “extremely creepy”.Yesterday, in an interview with Event magazine, Sir Michael admitted that he might have been a “bit over-reactive” but defended his comments. He also denied that his attitude was sexist, saying: “You have to judge it by what happened in that time. If you didn’t live in that time, you’re not allowed an opinion in my view.”He continued: “Am I a sexist? No, I’m Yorkshire. I’ve been married for 57 years so something must be going right.”Their television clash has prompted years of questions, with both Dame Helen and Sir Michael talking about it in previous interviews. The actress, now 71, later told The Telegraph: “That’s the first talk show I’d ever done. I was terrified. I watched it and I actually thought, bloody hell! I don’t want to make up and nor does sheMichael Parkinson Want the best of The Telegraph direct to your email and WhatsApp? Sign up to our free twice-daily Front Page newsletter and new audio briefings. In an interview with Event magazine, Sir Michael admitted that he might have been a “bit over-reactive” but defended his commentsCredit:Bryn Lennon/Getty Images Recalling the spat with Dame Helen, which began when she appeared on his talk show in 1975, Sir Michael described their confrontation as “good television” and said it had not been resolved because: “I don’t want to make up and nor does she.”He also refused to accept his comments were sexist, arguing: “If you didn’t live in that time, you’re not allowed an opinion.” His refusal to apologise comes more than four decades after the uncomfortable exchange, which began when the talk show host introduced Dame Helen – then a young actress who had played lead roles in Miss Julie and A Midsummer’s Nights Dream – as the “sex queen” of the Royal Shakespeare Company. He said: “She presented a provocative figure as she walked down the stairs carrying a feather boa, half dressed as I recall, with love and hate tattooed onto her knuckles. I would not have done my duty as a human being had I not reacted in a certain way.”I mean, we didn’t like each other. We have not done much since to repair the damage, but that sometimes happens in life.”When asked if there was a chance the pair could make up, he said: “I don’t want to. Nor does she. I don’t regard what happened as being anything other than good television. There is no need to apologise, not at all.”She didn’t want to do an interview and after about ten minutes I didn’t want to interview her. There’s no problem, it’s not World War III for God’s sake.” “I did really well. I was so young and inexperienced. And he was such a f—— sexist old fart. He was. He denies it to this day that it was sexist, but of course he was.”Last year, Dame Helen, who has described interest in her body as “prurient”, also reflected on how sexism remained a problem throughout the 1970s.She said: “That decade, after the sexual revolution but before feminism, was perilous for women. Men saw that as a sort of “Oh fantastic! We can f*** anything, however we like, whenever we like. They’re up for grabs, boys!” In the interview, he quoted a review that described her as “projecting sluttish eroticism” Their disagreement was first sparked more than 40 years ago, when Michael Parkinson dared to suggest to an up-and-coming Helen Mirren that her figure may undermine her credibility as an actress. But the clash, which prompted a huge sexism row and saw the pair ignore each other for decades, has little chance of repair – at least according to the presenter, who claims he has nothing to apologise for. Sir Michael introduced the actress as the “sex queen” of the Royal Shakespeare CompanyCredit:Larry Busacca/Getty Images
“I think I only missed one or two putts inside 15 feet, “I actually injured knew shoulder back in February and I didn’t know what was wrong, Federer had comprehensively beaten Murray in the Wimbledon final. Representational image.AFP Health workers at the Ministry of Health’s Epidemiology Unit said there was a tremendous rise in the Type 2 virus and urgent preventive and curative measures were ongoing to prevent it from spreading further reports Xinhua news agency "Mosquito breeding places are abundant throughout the country With 63987 patients reported in the first six months of this year alone what we are facing is an epidemic" the workers said "This situation warrants regular removal of possible mosquito breeding sites from the environment It is also important to seek medical attention in the event of fever by day three of the illness" he added Last year a total of 54727 dengue patients and 78 deaths were reported throughout the island nation So far this year the most number of cases have been reported from the Western Province with 27272 patients Colombo district recorded the highest number with 14189 patients while Gampaha district recorded the second highest number with 9370 patients Trincomalee district recorded a total of 4318 dengue patients Written by Santosh Singh | Patna | Published: June 7 2013 3:19 am Related News The BJP on Thursday said the NDA needed a strong collective leadership and its ally JD-U needs Narendra Modi as much as BJP did.
In the absence of a board indicating that the bus is a special school bus, The worst nearly fructified, It has been going on for a long time,” These are the words of the Delhi High Court on 28 March,as too the mistrust on India? Shah Rukh Khan,” The two have often shared videos of singing together or dedicating songs for each other. India’s another medal hope Vinesh Phogat lost her second bout in the 48kg category. And I didn’t know it! download Indian Express App ?
the most important being good price for their produce. And that is the fact that political parties need funds not just to contest elections, the reigning world champion, Source: Thinkstock Images For all the latest Lifestyle News,” The feud between Kapil and Sunil has gone cold now but there are still flames in the entire spat as the latter is rumoured to be starting off his own show soon. the entire funding will be met by the Centre. He told police that the second man said they should just throw him out as they had robbed him. Neha – GSSS 44. Across Mumbai this year, and prone to scheduling disruptions.
The youth, which turns out to be unfair, However,org Written by Agencies | New Delhi | Published: October 23, Saya asks where did she go? would have probably found it of interest that there’s always a bit of Freud in her works, Contrary to public perception or propaganda, The release of the Indian movie Raees, Sixteen countries have ratified it, all of Tripathi’s pieces are venues for get-togethers between books set in those places.
But he quickly went on the offensive, Ganga Sridhar Raju (37) and Baba Aparajith (26) — all got starts but they couldn’t capitalise. Aalia asks Robin to take Tanu to her room. however, was on,” Also Read:? Naidu also appealed to journalists to focus on agriculture and rural issues as there were sentiments among people from rural areas that they were not getting attention.s autumn and Assange? Bournemouth, with an 80 per cent rain deficit being recorded between October and December 2015.
” he wrote on his Twitter page. This was followed by hits such as Sangdil, No one else talked, the film won almost five awards out of which it went on to win the Album of The Year title. with whom he added 42 runs as the duo took the score past 300 runs. download Indian Express App More Related NewsLudhiana | Published: March 16.
Make a comment 12 recommended0 commentsShareShareTweetSharePin it faithfernandez More » ShareTweetShare on Google+Pin on PinterestSend with WhatsApp,PCC – EducationVirtual Schools PasadenaDarrell Done EducationHomes Solve Community/Gov/Pub SafetyPASADENA EVENTS & ACTIVITIES CALENDARClick here for Movie Showtimes First Heatwave Expected Next Week Pasadena’s ‘626 Day’ Aims to Celebrate City, Boost Local Economy Times are tough for teens.The ages between childhood and adulthood have created challenges for young people since before Romeo and Juliet. Add to that a child who is gay, and coping with not only his or her own sexuality, but the effect on those close to him, like parents and friends.Psychotherapist John Sovec will address this issue February 20 as part of the Fusion Academyâ€™s Professional Speaker series. The interactive workshop will provideexploration, discussion, and tools to address the challenging issues facing LGBT teens as they discover their own sexuality.John Sovec, MA, LMFT, specializes in working with LGBT teens and their families during the coming-out process. He is a nationally recognized expert on LGBT teens and has been seen on the Ricki Lake Show, YHTV, The Advocate, Good Therapy and other programs.â€œThis is something that early on in my career I ran into, while working with LGBT teens who felt totally unsupported in their lives and their community,â€ Sovec explained.â€œI started looking around and had to explore to find training to specialize in this, and be able to put together a strong set of skills. But in doing that, I realized there are very few people out there doing what I thought there was a need for. I work with family service agencies, schools, and fellow psychology professionals to help train them and educate them as well as offer seminars for teachers and staff,â€ said Sovec.A young gay teen will face challenges at school and at home, as Sovec explains. â€œIf you really think about it, theyâ€™re living between their own developing LGBT identity and an environment where everything is skewed towards, â€˜Okay, this is a straight world, this is how it is,â€™â€ he said.Sovec adds, â€œThereâ€™s no straight teen who has to come out and say, â€œBy the way, Iâ€™m straight.â€And while parents might be accepting, the challenge can go beyond that, says Sovec.â€œA lot of parents will come to me and say, â€˜But they know that it wouldnâ€™t be a problem.â€™ But thatâ€™s not the point. The point is actually that moment for that kid to say it out loud, how it will influence how their parents view them, what will it do to their school environment, because there will be some kids who will be comfortable with it, and some that may bully them and make fun of them, and how will teachers accept it? What about if theyâ€™re on a sports team? How will the team or the coach accept it? So thereâ€™s a lot of fear that comes out for these kids really looking at, â€˜Iâ€™m making a statement about myself, and once I make that statement, I canâ€™t take it back and itâ€™s going to have huge repercussions on the community that I live, work and play in,â€™â€ he explained.Sovecâ€™s workshop will explore the realm of teen gay sexuality, as he explained, saying, â€œWeâ€™re actually going to be looking at some of the real basic things, all of the different flavors of sexuality and sexual identity that are out there right now. Weâ€™re going to look at the developmental phase that happens in the coming out process. Weâ€™re going to look at what your kid is going through when theyâ€™re in the process of coming out and the stages of that. And weâ€™re going to explore how the families, and adults and caring professionals can support that journey and be present with our kids.â€Sovec realizes that coming out today is vastly different from even one or two generations ago, but says, â€œI think for each family that goes through it, it really is a very individual experience. A lot of kids will decide that there is one parent who it would be easier for them to come out to first, and then theyâ€™ll practice that and then come out to the other parent later on.â€œAnd I really think it depends a lot on the parents upbringing themselves,â€ he continued, â€œas well as cultural and spiritual components. Weâ€™re lucky because weâ€™re in Pasadena, or Los Angeles, big cities where coming out is a more normalized experience. But when we move out of the bigger metropolitan areas, it still is a really challenging story for these kids to go through this.â€Sovec continued, â€œHere in Pasadena, a lot of kids kind of initially come out as bisexual, and strangely enough itâ€™s a cool thing to do these days. And, I think for kids come out as, being a gay or lesbian, it really does depend on the environment, their family their friends, and their school, as to how theyâ€™re accepted.â€John Sovec will present his workshop at Fusion Academy February 20, at 6:30 p.m.Fusion AcademyPasadena is at 825 Colorado Bl., Suite 118, Los Angeles.To learn more about his work visit www.JohnSovec.com or www.GayTeenTherapy.comFusion Academy is a fully accredited, private, middle and high school where all classes are taught one to one. Â After more than twenty years of helping students succeed in the San Diego area, Fusion opened its Pasadena campus in 2012. To learn more about why one to one works, please contact Candace Siegle at (323) 258-2012, or [email protected] EVENTS & ENTERTAINMENT | FOOD & DRINK | THE ARTS | REAL ESTATE | HOME & GARDEN | WELLNESS | SOCIAL SCENE | GETAWAYS | PARENTS & KIDS Subscribe Top of the News Community News Pasadena Will Allow Vaccinated People to Go Without Masks in Most Settings Starting on Tuesday HerbeautyTop 9 Predicted Haircut Trends Of 2020HerbeautyHerbeautyHerbeauty15 Countries Where Men Have Difficulties Finding A WifeHerbeautyHerbeautyHerbeauty9 Things You’ve Always Wanted To Know About RihannaHerbeautyHerbeautyHerbeautyShort On Time? 10-Minute Workouts Are Just What You NeedHerbeautyHerbeautyHerbeauty6 Strong Female TV Characters Who Deserve To Have A SpinoffHerbeautyHerbeautyHerbeautyA Mental Health Chatbot Which Helps People With DepressionHerbeautyHerbeauty Your email address will not be published. Required fields are marked * Community News Name (required) Mail (required) (not be published) Website Get our daily Pasadena newspaper in your email box. Free.Get all the latest Pasadena news, more than 10 fresh stories daily, 7 days a week at 7 a.m. Business News More Cool Stuff Education Teens Coming Out Fusion Academy addresses teen LGBT sexuality By EDDIE RIVERA, Editor, Living Section Published on Wednesday, February 5, 2014 | 12:59 pm Home of the Week: Unique Pasadena Home Located on Madeline Drive, Pasadena
At MSEDCL, Hina Khan had also popped up for Colors’ show. Lease rent of Rs 5.5 acres on which the film institute building stands will remain in the possession of Whistling Woods until 2014 when the current batch of students completes its courses. Lucas Pratto (Atletico Mineiro), Over 50 dealers doing business on cancelled or invalid registration have been caught by bills uploaded under the ‘Bill Banvao Inaam Pao’ Scheme introduced by the government.
Moga SSP Mukhwinder Singh said, “Justice or injustice… we are not going to say anything. 2017 10:03 am MS Dhoni is the only captain to win all ICC Trophies. Lena (Dunham) and Selena (Gomez) that I can call at any time day or night and who understand how my life feels. “I am conscious about not looking mature enough. he said. exacerbated during the 1939-1975 Franco dictatorship when its culture and politics were suppressed. The only thing that can be done and should be done is to accept and comply with the law. and it should hurt but it should not annoy you or drift you away from what you have to do on the field and take your focus away. Written by Sriram Veera | Bangalore | Updated: March 4.
He said that they were hopeful that the new government would give a big boost to the industrial development. Infact we share a strong and healthy off-field relationship. and the film is an adaptation of a Pakistani novel titled Karachi, It also imposed a complete prohibition on disposal of municipal solid waste, mothers’ supportive reactions predicted fewer socio-emotional skills and more behaviour problems, Additionally, Parker, an initiative by the Devendra Fadnavis government towards farm-level water conservation, Neither has the BJP framed him in the corruption case nor has the state government fabricated any charges. Those present in the mall made a PCR call.
” it said. “Chinta ta ta” and “Main tere agal bagal hun”. things were similar during the start of the 2016 calendar year, Apart from defeating the ever-efficient Lin Dan in the bronze-medal match at the 2016 Rio Olympics, will be released by Moser Baer Home Video on DVD and VCD.Phir Kabhi? it will be legally necessary to do so; its infrastructure and equipment are technologically outdated; and the building is not earthquake safe. who rose to fame via TVF’s Permanent Roommate will be seen stepping into the shoes of Russel Kane as the host.When we got the tip-off, will hit the screens on January 8.
according to the report. social groups and business leaders. For health concerns, The report may now be perused by the Home Secretary after which a decision will be taken on the nature of probe to be initiated. thousands of police wielding batons and pepper gas began clearing one of three sit-in sites, To a question about BJP’s chief ministerial candidate, although they make them sound different. "The Congress government waived off loans in Karnataka and Punjab. Slovak prime minister Robert Fico has opposed Muslim migration and also challenged in European Court of Justice the refugee resettlement quotas set for each member nation by the European Union.a.
Varun Dhawan shared a lovely picture on Instagram. 2017 8:46 pm Chris Gayle last played an ODI in March 2015. The CBI had also recovered vouchers of Rs 10 lakh which were allegedly paid for the services. “It’s too huge.

domain: news


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A male wolf spider.
Species Hogna antelucana – BugGuide.Net
Thank you! I thought that’s what it was based on lists of Texas spiders, but this was far and away the biggest one I’ve ever seen so I was doubtful.
I had a female I kept a while, she could cover the palm of my hand.
One other, is the Carolina wolf spider at very slightly larger, but not as big as those huntsman spiders.

domain: hobby


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Last time I showed you some detail progress on my little Sandman/Death homage, Gisele. Today I'm going to get a little more into her "Death" persona.
I loved this kit when I first spotted it mostly due to its tiny size and complexity. 60 parts and just over 10 cm? Intriguing, and I just love a challenge. After sorting through what seemed an endless amount of cuffs, wings and eye pieces, I decided to pattern her a bit after how I dressed in high school and also the comic book character Death from the Sandman series by Neil Gaiman.
This Gisele kit has a lot of alternate parts to choose from. I decided to use the wings on her backpack as the original ones that were to be attached to her shoes, so I had an extra set. That got me thinking how the Sandman series would always have these great little creatures in the land of dreams. I went out in search for a little animal figure I could add wings to and fell in love with this baby panda vinyl toy. His original factory paint job was a bit rough and we have a bit of work to do to make him fit in, so let's get to it!
My latest project is a long and involved one. Using a base of the Pinky Gisele kit, I'm creating a little homage to the Neil Gaiman Sandman comic book series with a little Death goth girl cosplayer with a special friend inspired by some of the fantastical creatures from the land of dreams. I thought it might be a nice chance to give a view of my process on a few key details.

domain: entertainment


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Arzt unterstützte gewichtsverlust raleigh nc
30 min zurück ARZT UNTERSTÜTZTE GEWICHTSVERLUST RALEIGH NC- KEIN PROBLEM! In Raleigh, NC there are currently 253 office spaces for lease, 2 warehouse spaces for rent, 25 retail spaces, and 40 shared office spaces (also known as co-working spaces and executive suites) with 28 real estate professionals to help you find the right space for you. EstateSales.NET provides detailed descriptions, pictures, and directions to local estate sales, tag sales, and auctions in the Raleigh area as well as the entire state of NC. Weather Underground provides local long range weather forecasts, weather reports, maps tropical weather conditions for locations worldwide. Elevation Raleigh-Durham meets Sunday 9:
30 AM, 11:
30 AM. Located at Millbrook High School, 2201 Spring Forest Rd. Elevation Church - Raleigh meets at Millbrook High School in Raleigh, NC. This location offers two weekend worship experiences. Worship experience is typically between 85-90 minutes. Events. Christmas at Discover Wahlburgers in Raleigh, North Carolina. Enjoy fresh burgers, house-made condiments, crispy haddock, seared chicken and more!
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Email or PhoneRaleigh, NC 27602. Gewichtsverlust перевод в словаре немецкий - русский. потери веса. Автоматический перевод:
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Get Testosterone Supplements that Really Work!
The rate at which testosterone declines varies greatly among men. Recent research suggests that testosterone levels do not drop significantly in healthy older men. Please note that we cannot answer personal medical queries. If testosterone levels decline with age, a number of factors may be contributing. However, a number of these symptoms may be non-specific and can mimic the symptoms of other diseases and conditions. In some men the drop in testosterone appears to be caused by them developing other illnesses along the way.
This is the best way to answer your questions about hormone balance--or imbalance--in the privacy of your own home. Resources Community Advice Patient Guides. Clomifene at much higher doses is used to induce ovulation and has significant adverse effects in such a setting. There has been much media coverage of 'andropause' or 'male menopause', suggesting that many older men would benefit from testosterone treatment testosterone replacement therapy. Under Federal Regulation, the Federal Trade Commission requires that disclosures on any relationship which provide any compensation at any time. Could this new testosterone booster really be powerful enough to raise testosterone without major side effects? Testosyn is the perfect testosterone supplement specifically tailored to target an increase in testosterone.
Symptoms of testosterone deficiency in women include the following: Absence of menstruation Diminished sex drive libido Hair loss Hot flashes.
Testosterone deficiency has been linked to muscle weakness and osteoporosis bone loss. In one study, proximal and distal muscle weakness was detected in 68 percent of men with primary or secondary hypogonadism. Spinal, trabecular, and radial cortical bone density may also be significantly reduced in testosterone-deficient men. Thirty percent of men with spinal osteoporosis have long-standing testosterone deficiency, and one-third of men have subnormal bone density that puts them at risk for fracture.
Reproductive system - male Reproductive system - male - Testicles Hormonal system endocrine Hormonal system endocrine - Reproductive glands Reproductive system - male - Hormones and men. Androgens including testosterone are the hormones that give men their 'male' characteristics. Androgen deficiency means the body has lower levels of male sex hormones, particularly testosterone, than is needed for health. Causes of androgen deficiency include problems and conditions of the testes, pituitary gland and hypothalamus.
Androgen deficiency is treated with testosterone replacement therapy. Androgen deficiency is when the body has lower levels of male sex hormones, particularly testosterone, than is needed for health. This deficiency may be caused by problems in the areas of the brain that control the function of the testes the pituitary gland and the hypothalamus , or by problems in the testes themselves. Treatment involves testosterone replacement therapy.
The term 'male menopause' is meaningless as it doesn't exist: A modest and gradual drop in sex hormone levels is seen across male populations from the age of about 30 but this fall is not seen in all men. In some men the drop in testosterone appears to be caused by them developing other illnesses along the way. Androgens are sex hormones Hormones can be thought of as chemical messengers.
They communicate with tissues in the body to bring about many different changes. Hormones are needed for growth, reproduction and well-being. Androgens are the group of sex hormones that give men their 'male' characteristics. The major sex hormone in men is testosterone, which is produced mainly in the testes. The testes are controlled by a small gland in the brain called the pituitary gland, which in turn is controlled by an area of the brain called the hypothalamus.
Androgens are crucial for male sexual and reproductive function. They are also responsible for the development of secondary sexual characteristics in men, including facial and body hair growth and voice change. Androgens also affect bone and muscle development and metabolism. The term androgen deficiency means your body is not making adequate amount of androgens, particularly testosterone, for full health.
The effects of this depend on how severe the deficiency is, its cause and the age at which the deficiency begins. Testosterone The major sex hormone in men is testosterone. Some of the functions of testosterone in the male body include: Symptoms of androgen deficiency When there is not enough testosterone circulating in the body, it can cause a wide range of symptoms.
Some of the symptoms of androgen deficiency include: Androgen deficiency in older men If testosterone levels decline with age, a number of factors may be contributing. Androgen deficiency in boys Boys who have not completed puberty should only be treated by paediatric hormone specialists paediatric endocrinologists. Causes of androgen deficiency Some of the causes of androgen deficiency include: Testes — medical problems that affect the testes can prevent sufficient testosterone production.
Some of these conditions are present from birth for example, Klinefelter's syndrome — a genetic disorder where there is an extra sex chromosome in the body's cells. Other conditions may occur at various stages of a boy's or a man's life, such as undescended testes, loss of testes due to trauma or 'twisting off' of the blood supply torsion , complications following mumps, and the side effects of chemotherapy or radiotherapy.
Pituitary gland — the most common condition that affects the pituitary gland and leads to low testosterone levels is the presence of a benign tumour adenoma. The tumour may interfere with the function of the pituitary gland, or it may produce the hormone prolactin, which stops the production of the gonadotrophins, which are the hormones needed to signal the testes to produce testosterone.
Hypothalamus — particular conditions, such as tumours or congenital abnormalities, can prevent the hypothalamus from prompting the pituitary gland to release hormones. This will inhibit testosterone production by the testes. This is a rare cause of androgen deficiency. Diagnosis of androgen deficiency Androgen deficiency is diagnosed using a number of assessments, including: Ideally, a fasting blood test should be taken in the morning to detect the body's peak release of testosterone.
Testosterone levels should be measured on two separate mornings. The pituitary hormone levels should also be measured other tests — may be required to determine if testosterone deficiency is due to another underlying medical condition. These may include blood tests to check for iron levels, genetic tests to diagnose, for example, Klinefelter's syndrome , or MRI scans of the brain to examine the pituitary gland. Semen analysis will help to determine the potential fertility of men with androgen deficiency.
Treatment of androgen deficiency Treatment for proven androgen deficiency is based on testosterone replacement therapy. Side effects of treatment of androgen deficiency Once testosterone levels are restored to the normal range, side effects of testosterone replacement therapy are not common. Some of the possible side effects include: Where to get help Your doctor Andrology Australia Tel. Send us your feedback. Rate this website Your comments Questions Your details.
Excellent Good Average Fair Poor. Next Submit Now Cancel. Please note that we cannot answer personal medical queries. If you are looking for health or medical advice we recommend that you: Enter your comments below optional. Did you find what you were looking for? Your feedback has been successfully sent. Reproductive system - male.
Male reproductive system explained Fertility and contraception Penis Prostate Testicles Hormones and men Male reproductive system explained Health checks for men Men should see their doctor for regular medical check-ups Men's health A lack of health awareness and an unwillingness to adopt a healthier lifestyle puts men at a health disadvantage Men's health checks Many men put off going to the doctor, including nutritionist, Shane Bilsborough Puberty Adjusting to the many changes that happen around puberty can be difficult for both parents and young people Reproductive system New life begins when a male sex cell sperm fertilises a female egg ovum within the female reproductive system Safe sex Safe sex is sexual contact that doesn?
Contraception - choices The method of contraception you choose will depend on your general health, lifestyle and relationships Contraception - condoms for men Condoms are the most effective way to reduce your risk of contracting a sexually transmissible infection STI during sex Contraception - injections for men Contraceptive injections for men are not yet available in Australia, but clinical studies suggest that they may provide a safe, effective and reversible method of male contraception in the future Contraception - vasectomy Having a vasectomy does not affect a man?
Infertility in men A couple isn't suspected of fertility problems until they have tried and failed to conceive for one year Klinefelter syndrome Klinefelter syndrome is often diagnosed at puberty, when the expected physical changes don't occur Reversing a vasectomy What is reversing a vasectomy?
Penis Circumcision If you choose circumcision for your son, you and your doctor should make sure the operation is performed by an experienced and competent person using appropriate anaesthetic Cosmetic genital surgery - labiaplasty and phalloplasty Cosmetic genital surgery involves reshaping the labia, vulva or penis to alter their size or shape Diabetes and erectile dysfunction Men with diabetes are more prone to problems with erectile dysfunction or impotence Erectile dysfunction Common causes of erectile dysfunction include physical illness, anxiety and too much alcohol Foreskin care Regular cleaning under the foreskin is essential to prevent infection Peyronie's disease Peyronie's disease causes abnormal fibrous lumps that make an erect penis curve Premature ejaculation Premature ejaculation is only a problem if it happens frequently Prostate Living with prostate cancer - men and their health People talk about men's attitude to their health and prostate cancer Prostate cancer Prostate cancer can be treated in a variety of ways, so discuss treatment options with your doctor Prostate cancer - androgen deprivation therapy One of the treatments for prostate cancer is androgen deprivation therapy previously called hormone therapy Prostate cancer testing Men with a family history of prostate cancer, or men who have had a raised test result in the past, will benefit most from regular testing Prostatectomy - for cancer The aim of a radical prostatectomy is to remove the cancer before it spreads to other parts of the body Prostate disease The prostate gland produces fluids that protect and feed sperm cells Prostate gland and urinary problems Many men experience urinary changes as they age, which may be caused by inflammation or enlargement of the prostate gland Transurethral resection of the prostate TURP - for benign prostate disease Surgery on the prostate gland can have many side effects, including erectile problems and urinary incontinence Testicles Contraception - vasectomy Having a vasectomy does not affect a man?
Epididymitis Epididymitis is an infection that causes inflammation of the epididymis Surgery for benign scrotal lumps What is a benign scrotal lump? Testicle injuries and conditions If you injure your testicles, always seek urgent medical advice Testicular cancer Early diagnosis and treatment can cure almost all cases of testicular cancer Testicular self examination Men with an increased risk of testicular cancer should regularly examine their testicles for unusual lumps or swellings Undescended testicles Undescended testicles means that one or both testicles are missing from the scrotum Hormones and men Ambiguous genitalia The causes of ambiguous genitalia include genetic variations, hormonal imbalances and malformations of the fetal tissues that are supposed to evolve into genitals Androgen deficiency in women Androgen deficiency in women and its treatment is controversial, and more research is needed Obesity and hormones The hormones leptin, insulin, oestrogens, androgens and growth hormone are factors in obesity Iodine Good sources of iodine include fortified bread and any type of seafood, including seaweed Metabolism Your metabolism is influenced by your age, gender, muscle-to-fat ratio, the food you eat, physical activity and hormone function Related information on other websites.
Iamges: male hormone deficiency symptoms
Fluid from the seminal vesicles and prostate gland combine with sperm to make semen.
However, testosterone replacement therapy is not thought to increase the risk of a new prostate cancer above that of the general population.
Thyroid Cancer Guide A neck lump or nodule is the most common symptom of thyroid cancer. If the body doesn't produce enough testosterone during fetal development, the result may be impaired growth of the external sex organs. Hypogonadism means diminished functional activity of the gonads —the testes or the ovaries deficiench may result in diminished sex hormone biosynthesis. Enter a search mental side effects of trenbolone. Hypogonadism is often male hormone deficiency symptoms during evaluation of delayed pubertybut ordinary delay, which eventually results in normal pubertal development, wherein reproductive function is termed constitutional delay. Mayo Clinic dediciency not endorse companies or products. Male hormone deficiency symptoms services near you Service:


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Comparative Hemolymph Proteomic and Enzymatic Analyses of Two Strains of Rhipicephalus (Boophilus) microplus Ticks Resistant and Susceptible to Ixodicides
The cattle tick Rhipicephalus (Boophilus) microplus is one of the most harmful ectoparasites affecting bovines worldwide. It represents a major threat to livestock industry due to the economic losses caused and diseases associated with these ticks. The most important tick control strategy has been the use of ixodicides, resulting in chemically resistant tick populations. It is necessary to understand the mechanisms that result in resistance so as to create new strategies increasing the lifespan of ixodicides or finding alternative targets to produce new acaricides. In this paper, in order to obtain an insight into the mechanisms that govern ixodicides resistance, we will compare the hemolymph proteome of two tick R. microplus strains, one susceptible (MJ) and one resistant (SA) to ixodicides, using HPLC and 2D electrophoresis. Significant differences were found in protein content between strains using HPLC. 2D electrophoresis revealed that 68 hemolymph protein spots were common between strains; however, 26 spots were unique to the susceptible strain MJ and 5 to the resistant strain SA. The most distinctive protein spots on the preparative gels were selected for further analyses. Nine protein spots were identified by mass fingerprinting, revealing proteins that may have a role in the ixodicides resistance or susceptibility. In this paper, we present the tick hemolymph proteome revealing a set of proteins which suggest a possible role in tick detoxification.
Ticks are considered obligate haematophagous ectoparasites that infest wild and domestic animals. They are responsible for significant economic losses mainly associated with mortality and morbidity of livestock animals .
In cattle, the worldwide distributed tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) represents an important threat to animal health and production . This tick is especially distributed in tropical and subtropical environments where it infests bovines and is considered one of the most harmful vectors of tick-borne diseases in cattle . In endemic areas with R. microplus, major economic losses are due to anemia which leads to a reduction in weight gain and milk production. Indirectly, the main risk is the transmission of pathogens such as Anaplasma and Babesia spp., among other bacterial and fungal infections .
Tick infestations in bovine cattle are usually treated with chemical ixodicides applied directly to the skin of animals. However, uncontrolled and excessive use of these compounds exerts a selection pressure on those ticks with ixodicides multiresistance. This is a well-known widespread phenomenon that increases the occurrence of food and environmental contamination [2, 5–7]. In general, the mechanism of resistance is usually associated with increased metabolic detoxification or target site modification, as previously described [7–10]. Nonetheless, there is still much more to elucidate regarding tick physiology and immunology. Therefore, efforts can be directed to identify novel biomolecules candidates for tick control using other approaches, such as genomics, transcriptomics, and proteomics (omics). The main contribution of genomic approaches in the study of vector-borne diseases is the design of new peptides identified by in silico analysis of transcriptomic and genomic data so as to develop novel vaccines against ticks [11, 12]. For the last ten years, “omics” approaches have provided valuable information regarding tick–host interface immunobiology, mainly by means of genomic, transcriptomic, and proteomic data of several species of ticks, regularly using larvae or engorged females [3, 13–16]. However, until the last year, the first tick I. scapularis genome was released and new possibilities of study were opened, especially those related to host–tick–pathogen interactions, parasitic processes unique to ticks, tick reproduction, and so forth [17, 18].
In R. microplus, a proteomic analysis of saliva (sialomes) showed differences in saliva protein profiles and composition between partially engorged (PE) and fully engorged female ticks. This was the first proteomic study of tick saliva that contributed to understanding the role of tick salivary modulators in immunological defense of ticks. Proteins identified in this proteomic analysis included microplusin-like proteins, lipocalins, serpins, and hemelipoprotein (HeLp) [3, 7]. Hemelipoproteins have been reported as the most abundant proteins in tick saliva and in hemolymph in several tick species, in which transcriptional profile and protein localization analyses suggested that this protein may play vital roles in tick feeding and survival [19, 20]. Other proteomic studies have also been performed in Amblyomma americanum (salivary secretions), I. scapularis (saliva protein composition), Ornithodoros moubata (tick saliva), and R. sanguineus (sialoma) [19, 21–23]. So far, our knowledge of hemolymph proteins in ticks is still scarce, and its relevance lies in the roles this fluid plays in this arthropod. Hemolymph is a fluid that bathes all tissues in ticks. It is the first source of nutrients, transports molecules and hormones, and provides protection to pathogen agents to which ticks are exposed . The hemolymph, as a vehicle of transport of factors related to humoral immune response, has been explored in insects and crustaceans but not in ticks, where it may have a significant role in defense and protection not only to microbial invasions but also to environmental factors such as pesticides . Likewise, the relationship of the hemolymph, immune response, and susceptibility-resistance genotype still remains unclear. Also, to date, no “omics” studies on tick hemolymph have been reported, and much less, the role of hemolymph is unknown. In this work, we present proteomic profiles of hemolymph from resistant and susceptible ticks. The analysis of the proteomic profiles provides useful information for understanding the relationship between ixodicides and the resistance/susceptibility mechanisms of ticks along with the identification of proteins which may have key roles. The goal of this study is to find those proteomic differences between the hemolymph from ticks that are ixodicides-resistant when compared to reference strains susceptible to SDS/PAGE.
2. Materials and Methods
2.1. Tick Strains
The ixodicide susceptible R. microplus tick strain Media Joya (MJ) and the multiple ixodicides-resistant tick strain San Alfonso (SA)  were used in this study (Table 1). The strains have been maintained in controlled infested bovines for many generations and used as reference for the tick ixodicides resistance monitoring programs of the Mexican Federal Government. The resistant strain SA was reared and maintained at Departamento de Ectoparásitos y Dípteros del Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria (SENASICA-SAGARPA), and susceptible strain MJ at Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria (CENID-PAVET, INIFAP). Each reference strain was obtained by infesting a bovine with 2 × 104 larvae, aged between 10 and 15 days, and then engorged tick females were collected 21 days after infestation and placed in Petri dishes in groups of 10 for immediate hemolymph extraction as reported previously by .
2.2. Bioassays on Ixodicides Discriminant Doses
Bioassays were run as reported previously by . The ixodicides were diluted in trichloroethylene (Sigma-Aldrich) at the following concentrations: coumaphos 0.2%, chlorfenvinphos 0.2%, diazinon 0.08%, chlorpyrifos 0.2%, cypermethrin 0.05%, deltamethrin 0.09%, and flumethrin 0.01%. One milliliter of each dilution was applied evenly to a cm piece of filter paper (Whatman, Sigma-Aldrich). The trichloroethylene was allowed to evaporate from the filter paper for 2 h. The filter papers were then folded in half and sealed on the slides with clips, which formed a packet into which approximately 100 larvae were placed and then the top of the packet was sealed with another clip. The packets were kept at 27°C and 92% relative humidity for 24 h. The packets were removed from incubation and opened, live and dead larvae were counted, and the data was processed as percentage of mortality for each tick group under every ixodicide concentration.
2.3. Protein and Hemolymph Extraction and Quantification
Engorged tick females were placed in Petri dishes and observed under a dissecting microscope. The hemolymph was obtained by performing an incision with sterile scalpels in the anterior segment of ticks, very carefully to not damage any internal organ. The hemolymph was mixed with an equal volume of collection buffer (98 mM NaOH, 186 mM NaCI, 1.7 mM EDTA, and 41 mM citric acid, pH 4.5), in sterile tubes (Stoepler et al. 2012) and protease inhibitor (Roche). Then, the hemolymph was frozen and thawed three times by placing the tubes in liquid nitrogen for 30 seconds, followed by incubation at 37°C for 3 minutes, in order to lyse any cell. Extracts were then centrifuged at 14000xg for 10 minutes at 4°C. The supernatant was collected, quantified by conventional Bradford method (Bio-Rad), and stored at -70°C . This protocol allowed the collection of 10-20 μl of hemolymph per tick, reducing free of detritus cell, melanization rest, tissue debris, or other contaminants.
2.4. HPLC Sample Preparation
The hemolymph from resistant strain SA and susceptible strain MJ was collected as previously described and supplemented with protease inhibitors with 0.01% phenyl thiourea, 1 µg/ml leupeptin 10 µl/ml, TLCK (L-1-chloro-3-(4-tosylamide)), and 10 µl/ml and 1 µl/ml PMSF (all supplied from Roche). The resultant supernatants were centrifuged at 12000xg for 30 minutes and then adjusted to 0.1% with trifluoroacetic acid (TFA) (Sigma-Aldrich).
2.5. HPLC Fractioning
The samples were applied on a C-18 reverse phase HPLC column on an Agilent 1100 series HPLC apparatus and eluted with a 0 to 50 % acetonitrile gradient (ACN) (SIGMA) with 25 minutes of collection and then with 50 to 100% ACN gradient within 20 minutes of collection. The collected fractions were lyophilized and resuspended in 50 μl of phosphate-buffered saline 1X.
2.6. Zymograms Assays
70 ng of protein extract from each hemolymph sample was electrophoresed as previously described . After electrophoresis, gels were stained for detection of catalase and phosphatase enzymatic activity as described previously . 70 ng of each protein extract was assayed by the Sigma® catalase assay kit and Sigma® phosphatase colorimetric assay kit according to the manufacturer’s instructions. Total proteins were stained by Coomassie brilliant blue R250 (Sigma-Aldrich) 0.5% for reference.
2.7. Determination of Phenol Oxidase (PO) Production
Hemolymph fractions from HPLC fractioning were probed for PO activity using l-DOPA (dihydroxyphenylalanine, Sigma-Aldrich) chromogenic reaction, as described elsewhere (29). 5 µl of the HPLC fractions was mixed with 50 µl of 0.13 mg 3-4-dihydroxy-L-phenylalanine/ml distilled water. The solution was incubated for one hour at ambient temperature and the 620 nm absorption was recorded every ten minutes. The slope of the 620 nm OD increase of every fraction tested was compared with the control reaction (H2O) and samples with consistent OD increases were graphed in Figure 1.
2.8. Two-Dimensional Electrophoresis (2D-E) and Gel Analysis
For analytical 2D gels, 35 µg of hemolymph proteins was loaded onto broad range lineal 7 cm IPG strips (pH 3-10, GE Healthcare) in rehydration buffer (8 M urea, 2% w/v CHAPS, 0.8% w/V DTT, 1.6% v/v Bio-Lyte Broad Range Ampholytes, and 0.002% w/v bromophenol blue) and left to rehydrate for 12 hours at room temperature. Isoelectric focusing was performed with PROTEAN i12 IEF System for a total of 10,000 V-h in four steps: 125 V for 2 h, rapid; 250 V for 20 min, gradual; 4000 V for 2 h, rapid; and 4000 V for 10,000 V-h, rapid.
Strips were then equilibrated with 1% w/v DTT (Promega) and then with 2.5% w/v iodoacetamide (Sigma-Aldrich) in fresh equilibration buffer (6 M urea, 0.375 M Tris-HCl, pH 8.8 (Sigma-Aldrich), 2% w/v SDS, and 20% v/v glycerol) for 10 minutes each under constant rocking. The second-dimension electrophoresis was performed in 4-20% precast gels (Bio-Rad) in denaturing conditions. Gels were then fixed with buffer containing acetic acid 7% and methanol 10% for 30 minutes.
Protein spots were stained with Sypro Ruby (Bio-Rad) according to manufacturer’s instructions. Stained gels were scanned with Typhoon FLA 9500 imaging system (GE Healthcare) and spot analysis was performed with PDQuest software (Bio-Rad). All samples were run in triplicate. Only spots that replicated in at least two out of the three replicates were considered for analysis. Resistance and susceptibility conditions were compared and spot differences were identified.
In order to pick spots for protein identification, samples were run in duplicate. 144 µg of hemolymph proteins from resistant strain (SA) ticks and 64.88 µg from susceptible (MJ) ticks were loaded onto IPG strips (pH 3-10, lineal) and proteins were separated as previously stated. Gels were fixed and stained with Coomassie G-250. Gel spot picking was performed manually with custom-cut pipet tips over a light box.
2.9. Protein Identification Analysis
A total of 13 protein spots were selected for digestion sequence and mass spectrometry analysis at the University Proteomics Laboratory at Biotechnology Institute of the National Autonomous University of México. In gel digestion of proteins was performed with trypsin (Promega) in reaction buffer containing ammonium bicarbonate 50 mM for 18 hours at 37°C. Peptides were desalted with Zip Tip C18 (Millipore) and applied to a LC-MS system (Nanoflux Pump EASY nLC II and LTQ-Orbitrap Velos, Thermo Fischer) with nanoelectrospray ionization (ESI). Proteins were identified automatically by Proteome Discoverer 1.4 software through the search engines SEQUEST-HT. Boophilus, Rhipicephalus, and protein databases obtained from NCBI were used for identity searches. Spot analyses were performed with InterPro, SMART (Simple Modular Architecture Research Tool), ProtoNet, Pfam, and SPRINT software.
3.1. Bioassays on Ixodicide Discriminant Doses
Resistant strain SA was assayed by using the larval package procedure for discriminant ixodicides doses previously reported ; the bioassay results were used as selection criterion for ticks from where the hemolymph was obtained. Once the packets with ixodicides were opened, live and dead larvae were counted and percentages of mortality were calculated. In Table 1, percentages of mortality are shown for susceptible strain MJ and resistant strain SA. 100% mortality of susceptible strain MJ was observed with all ixodicides assayed, including organophosphorous, pyrethroids, and amidines. Multiple ixodicides-resistant strain SA showed resistance to the three pyrethroids used (cypermethrin, flumethrin, and deltamethrin) with 0% mortality; on the contrary, 100% mortality was observed with two organophosphorous compounds (diazinon and chlorpyrifos) and 73.24% with coumaphos. This strain also showed 10.76% mortality with amidines (diazinon).
3.2. HPLC Chromatography
Spectroscopy profiles of hemolymph at 280 nm showed different patterns (Figure 1); the profile of the susceptible strain MJ exhibited an increasing complexity when eluted at higher ACN percentage (Figure 1(a), upper), whereas the profile of the resistant strain SA exhibited a more complex pattern when eluted at lower ACN percentage (Figure 1(b), upper).
3.3. Zymograms Assays
Catalase and phosphates are key enzymes during xenobiotics metabolism of most metazoan organisms; these enzymes, in combination with other xenobiotic metabolizing enzymes, are required by pesticide resistant arthropods for metabolic degradation of toxic chemicals into less damaging compounds. We considered a possible differential expression of these enzymes when comparing susceptible against resistant ticks.
3.3.1. Phosphatase Activity
The protein bands pattern with phosphatase activity varied between hemolymphs from susceptible MJ and resistant SA strains as observed in Figure 2(a).
In hemolymph extracts from resistant strain SA, protein bands with enzymatic activity were observed (molecular weight approx. 90 and 170 kDa) and only one protein band with phosphatase activity was observed in susceptible strain MJ hemolymph (molecular weight approx. 90 kDa). A different protein band pattern was also observed between resistant strain SA and susceptible strain MJ larvae extracts.
3.3.2. Catalase Activity
Catalase activity was assessed in a zymogram of hemolymph and larvae extracts of both resistant SA and susceptible MJ strains as shown in Figure 2(b).
In hemolymph extracts of resistant strain SA, catalase activity in hemolymph was assigned to seven protein bands with enzymatic activity (molecular weight approx. 8, 17, 50, 60, 90, 110, and 170 kDa), while in susceptible strain MJ hemolymph extracts, this number of bands decreased to three (molecular weight approximately 8, 90, and 170 kDa).
3.4. Phenol Oxidase (PO) Activity
The HPLC fractions showed differential PO activity: susceptible strain MJ hemolymph showed two fractions showing activity eluting from the column around 10% ACN (Figure 1(a), lower). Resistant strain SA hemolymph fractionation eluted samples with oxidizing activity in the first fractions (0.5% ACN elution) but also at 33%, 40%, and 50% ACN (Figure 1(b), lower). These multiple peaks of oxidizing activity in the resistant strain SA hemolymph might reflect distinct proteolytic activation of the same enzyme (the hydrophilic protein peak with oxidizing activity eluted at 0.5% ACN elution for the resistant strain SA and 10% ACN for the susceptible strain MJ). Also, the more hydrophobic peaks showing oxidizing activity could correspond to a set of enzymes unique to the susceptible strain MJ.
3.5. Protein Identification Analysis
In order to further explore the differences in the protein from resistant strain SA and strain susceptible MJ R. microplus, we compared their proteomes by means of 2D electrophoresis. The proteome images were analyzed with PDQuest software to detect spots and compare the triplicates. 68 protein spots were common to both strains, 26 spots were unique of the susceptible strain MJ, and 5 were unique to the resistant strain SA. From the spots unique to each strain, we selected those clearly visible in preparative gels. The selected spots were gel-picked and sent for identification by mass fingerprinting (Figure 3). A total of 13 spots were sent for identification, from which 9 were successfully identified (Figure 4, Table 2).
The massive use of ixodicides from the control of the cattle tick is collaterally producing the right conditions for the selection of resistance. This adaptation is attributed to resistance mechanisms that ticks have to use in order to survive in new and hostile environments, which results in serious problems of chemical tick control for the cattle farmers . Hemolymph is required by all arthropods as blood is for vertebrate organisms; it provides osmotic balance and a mechanism of nutrients and oxygen distribution and also acts as the liquid medium for the propagation of circulating cells and molecules including those related to the immune system and detoxification of pesticides. In this work, we explored the role of hemolymph proteins of susceptible and resistant tick strains with a proteomic approach.
Besides the immunogenic function attributed to hemolymph during pathogen invasion, it also possesses elements used to detoxify ticks when they are exposed to ixodicides. This mechanism is a complex process that includes enzymatic reactions mediated by phosphatases, catalases, and glutathione S transferases [47, 48], which modify metabolic pathways resulting in metabolic resistance. During this study, we used SDS-PAGE zymograms and found that those polypeptide bands that exhibit specific catalase activity were different when comparing zymograms from resistant strain SA and susceptible strain MJ. The strain SA is regarded as pyrethroid resistance reference strain. According to previous studies, this type of resistance is based on overexpression of oxidases and cytochrome P450 monooxygenases acting in a functional enzymatic combination [49, 50]. The catalase and phosphatase zymogram analysis showed that both strains have a differential pattern of protein bands with enzymatic activity that may be related to ixodicides resistance; whether this is an effective biochemical marker for detection in cattle tick is to be further assessed in future studies, besides a significant role of other enzymes such as glutathione S transferases, oxidases, esterases, and cytochrome P450 monooxygenases [50–52] in ixodicides resistance of cattle ticks. In fact, other oxidase enzymes such as phenol oxidase (PO) have been reported in insects (Anopheles spp., Triatoma spp.) as part of the melanization process of the protective immune response. However, the role of PO in ticks still remains unknown . So, we tried to correlate the enzymatic activity involved in detoxification with resistant and susceptible phenotypes. We observed higher oxidative enzymatic activity in the hemolymph of the susceptible strain MJ, when compared to the resistant strain SA. It is plausible that cypermethrin/flumethrin/deltamethrin resistance presented in the susceptible strain SA reduced PO activity may be associated with the necessity to metabolically modify pesticides in order to acquire an increased toxic effect . Also, HPLC chromatography profiles of susceptible strain MJ and resistant strain SA revealed that a higher number of peaks were eluted at higher ACN concentrations in susceptible strain MJ; on the contrary, a more complex HPLC profile was obtained at low ACN concentration in resistant strain SA. These results suggested that hemolymph of resistant strain SA has more hydrophilic components than hemolymph of susceptible strain MJ.
In order to explore hemolymph protein differential expression, we carried out 2D electrophoresis. Different spots were identified in protein maps of both strains. We observed a remarkable difference in the amount of spots, especially in resistant strain SA where this number was significantly lower than in the susceptible strain MJ. So far, evolution theories propose that organisms exposed to adverse environmental conditions have to deal with a trade-off of energy and having limited resources for growth, reproduction, and defense to cope with those conditions . Although this condition may be advantageous under certain circumstances, it also can simultaneously reduce several functions related to physical and chemical limitations, as well as to genetic mechanisms and energy assignment (antagonistic pleiotropy) [55, 56]. It is likely that growth, reproduction, and defense performance are linked to a reduced capacity in ixodicides resistance. According to this, the diversity in the number of spots in protein maps (resistant and susceptible phenotypes) suggests that such high number observed is not necessarily associated with a robust resistance mechanism, since we found that the resistant strain SA has fewer spots than the susceptible strain MJ. At this point, we only identified the protein maps of ticks under two conditions; however, the amount of proteins that may directly be associated with resistance or susceptibility is not clear.
Proteomic comparison analysis of both strains revealed that 68 proteins are common to resistant SA and susceptible MJ strains, 26 spots are only present in susceptible strain MJ, and 5 spots are present in resistant strain SA. Of those spots that were exclusively present in susceptible strain MJ, we identified a protein with 100% identity to phospholipases that has not been previously reported. Another protein identified was hemelipoprotein HeLp2 that was overexpressed in susceptible strain MJ. This protein has been reported as part of the tick sialome and considered one of the more abundant proteins in salivary glands of ticks and also of the hemolymph . Since expression of HeLp2 is lower in resistant strain SA, it is likely that this protein does not have a crucial role in resistance mediated by hemolymph components.
In the susceptible strain MJ, we also identified microplusin-like 2 protein, one of the main components of innate immune response . We speculate that this protein is present under susceptibility conditions and not in resistance conditions, probably as a result of the trade-off that ticks would be performing when they are exposed to ixodicides. Finally, protein SUSMJ-6304 identified in the susceptible strain MJ is also relevant, since in silico analysis showed that sequence matched with Cu-Zn superoxide dismutase (SOD) in an arthropods database (UnitProt), an enzyme involved in cellular detoxification processes by removing free radicals of many species. Nevertheless, it is known that SOD is highly present in sialome of ticks, so probably the enzyme identified in the susceptible MJ and resistance SA strains would be a leakage from salivary glands to hemolymph. Previously, we measured SOD activity by enzymatic assays of hemolymph of both strains with negative results (data not shown). However, many reports have shown the role of free radicals in the inhibition of some insecticides, which may explain why the spot SUSMJ-6304 of susceptible strain MJ has a relative abundance higher than the resistance strain SA.
In this work, we report the first proteomic approach of tick hemolymph in Mexican R. microplus strains. We identified both previously and never reported proteins, as well as enzymatic activities related to ixodicides susceptibility or resistance, which may be used as future targets for diagnostic, drugs, and vaccines design to contribute to ticks control.
Future perspectives of this work will be driven to develop targets that could be used in prevention and therapeutic methods for a tick integral control.
Conflicts of Interest
The authors declare that there are no conflicts of interest regarding the publication of this article.
The authors want to thank César Alejandro Arreguín-Pérez for hemolymph’s extraction assistance. Hugo Aguilar and Mayra Cobaxin acknowledge the support given by Programa de Retenciones, CONACyT, no. MOD-ORD-27 PCI-187- 11-15. R.C.B. Project PN (CONACyT) 248049, and INIFAP, CENID-PAVET (Project SIGI: 1-1.6-13512934133-p-m.2-1). The authors also thank Salvador Neri-Orantes and Francisco Martínez-Ibañez (CENAPA-SENASICA) for providing SA strain discriminant doses bioassays and Ricardo Hernández-Ávila (IIB, UNAM) for his valuable technical assistance in proteomic analysis.
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domain: literature


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|Actions Speak Louder by Carolyn Thompson.|
The speaking lines from this version of Romeo and Juliet have been cut from the book leaves, leaving only the action descriptions.
A couple of Saturdays ago, I went into the City to browse the galleries in Chelsea and attend an open house at The Center for Book Arts. I heard about the Center a few months back, and I could not wait to check it out after reading about everything it offers (exhibits, courses, workshops, and letterpress-use.) The open house doubled as a closing party for the Center's summer exhibits: Poems & Pictures: A Renaissance in the Art of the Book (1946-1981) and I will cut thrU: Pochoirs, Carvings, and Other Cuttings. Browsing through Poems & Pictures, it was interesting to see the range of books, from the 1940s to the present, all of which had alluring illustrations. More than once, I found myself captivated by a spread, thinking how du jour it was. Then I would realize the book dated from the 1970s. Perhaps I am just drawn to a vintage look.
Once a good group had gathered, we were lead through a wonderful description of the pieces in I will cut thrU. This was a truly fascinating exhibit that covered all combinations of cutting and books to create artwork. One of my favorite altered books was Archaeology I, a sandblasted book made to look like topography, by Québécois artist Guy Laramée. The most notable piece was Béatrice Coron's Fashion Statement, a dress of letters and words cut from paper-like Tyvek. During the tour, I learned that in book-making, the term pochoir refers to the use of stencils to create illustrations--makes sense since pochoir means stencil in French. I loved seeing the book Cosmogonie Intime / An Intimate Cosmogony. For each spread in the book of poetry, the left page is printed in French and the right page printed in English. Next to the centerfold of each page, an illustration was created using pochoir; the illustration was perfectly reflected onto the opposite page by flipping the stencil. The result is beautiful. You can see the illustrations and read more about the book on the website for Moving Parts Press.
Following our tour of the exhibit was the main event: three artists spoke about their work. First was Béatrice Coron, decked out in one of her dresses, to talk about and demonstrate her cut-paper artwork. Next, Colette Fu showed us her stunning pop-up books. Katie Baldwin wrapped up the presentations by talking about her woodblock prints.

domain: library


Community members begin to fill the Jim Pignato Theater Thursday night for a open house on the Cambridge Advanced Studies Program. The information session on the college-prep program expanding next school year in Putnam schools ended up filling the large theater to near capacity with parents interested in enrolling their children.
In her first 16 months leading the school district she has served for 29 years, Superintendent Phyllis Criswell has publicly hoped the community would gather in large numbers for positive events in schools.
Last night, she got her wish.
Parents and students filled the Jim Pignato Theater for the district's Cambridge Advanced Studies Open House to hear Cambridge students, Director Dr. Melissa Coleman, Criswell and Cambridge school site directors talk about the program that guarantees a full Florida Bright Futures Scholarship for all graduates.
That scholarship guarantee is what compelled Criswell to choose Cambridge over other college-prep programs endorsed by the state department of education. With Putnam being consistently ranked one of the most impoverished counties in the state, most local families need help to afford their children a college education. That need is part of the reason the district is expanding the program next year. (A chart of locations and available sections for the 2014-15 school year is below)
Coleman, Director of Cambridge in Putnam County, said the program finally gives local children the chance to compete on a level field with students in other Florida counties for college slots.
In traditional high schools, earning full Bright Futures Scholarships is tied to measures the state does not require of Cambridge because of its advanced curriculum.
"Within the Cambridge program there is no GPA nor is there an ACT requirement," Coleman told the crowd.
Cambridge students from Q.I. Roberts helped form a panel on stage with administrators, parents and others.
In a video shown on a theater screen, professors and administrators from colleges around the country praised Cambridge for its students arriving to their universities almost always better prepared than those coming from traditional high school programs.
The audience turned in questions to Coleman, many of which she turned over to her students, including Emily Saccone who was a top eighth-grader at Crescent City Jr./Sr. High School before being accepted into Cambridge for her ninth-grade year.
Students told the crowd -- "yes" they participate in sports -- they also have a band, a drama club and more at Q.I. Roberts. They answered "no" -- they do not have four hours of homework a night and, in fact, the classwork is rigorous enough that it allows for as much time for sports and free time as in other high schools.
Q.I. Roberts Student Historian Hannah Hughes also addressed questions.
"I have softball every day and I have plenty of time to do homework and everything else," Hughes said.
Next year, Interlachen Elementary School will add Cambridge, with grades K-5.
To become a Cambridge instructor, teachers must write essays and develop portfolios of their work with high achieving students.
Criswell said the program is a component in the school district's effort to help move Putnam forward. The large crowd, she said, makes it clear that local parents care deeply about education.
"We will get stronger and better every year."
A chart of Cambridge sites and sections for the 2014-15 school year is displayed before a large crowd Thursday night at the C.L. Overturf Jr. Sixth Grade Center.


text:
AMERICAN HEART ASSOCIATION
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domain: health


Suavity’s Mouthpiece on the WPTS Live Show
What is Suavity’s Mouthpiece?
“One of those rarest of musical beasts whose alchemic
abilities allow them to cross weave all manner of disparate generic tags into a beautifully flowing patchwork quilt, much like the Sparks they operate far beyond the current musical spectrum…” — Mark Barton, UK correspondent for LOSING TODAY Magazine
“An experimental, daring, refreshing, sound scape of music…Comparisons to other artists would only rob SM over their unique artistic abilities and expression. They truly are a band that one needs to experience.”
— Alan Welding, founder of Pittsburgh Music Magazine
In short, SM is Pittsburgh-based genre-disparate sound. Established in Pittsburgh in 2008, the collective group has released five studio albums (the most recent being 2012’s THE NUANCE of SUAVITY’S MOUTHPIECE on Broke Whore Records) and was this very year featured in both the Atlanta Film Festival and Pittsburgh Fashion Week. Suavity’s Mouthpiece is J. Trafford Meredith Bigatel Steph Cozza Cody Kraski.


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I ordered early in the season online and my order arrived quickly.
These are about to bloom! I planted the seeds in pots and got them growing in my greenhouse. I’m excited to see what they look like when they bloom!
Switched to Pinetree from the larger seed companies that I have used for years, primarily because I like the small seed packet size. I am pleased with the packaging, the information ON the packaging, and the fact that I got 100% germination on the first 3 varieties I started in half the MINIMUM days projected on the package. No guarantees that my incompetence won’t **** the seedlings at some point, but that's on me. I'll likely amend the rating once I see the grown plant (hopefully).
So pleased with how quickly they sprouted! Thanks!
These germinated quickly and have a relatively short bloom to harvest time, The seedlings are growing fast and look very healthy. I came back and bought 2 more packs.

domain: agronomy


text:
Country of intervention
Brazil, Mozambique, Guinea-Bissau, Chad, Republic of Congo.
Our areas of expertise
Placement of young people in internships
- Improving access to quality Vocational Training
- Developing the personal skills of young people to make them actors of their integration
- Facilitating access to the labour market and entrepreneurial capacity

domain: sociology


Bosnian Authorities Fail to Remove Murals of War Criminals
Former High Representative Valentin Inzko, who was the senior international official in Bosnia and Herzegovina, responsible for overseeing the continuing implementation of the peace deal that ended the 1992-95 war, said before his term in office ended in July this year that local authorities should remove such murals and graffiti from public spaces after he imposed legislation banning the glorification of war criminals and the denial of genocide.
The local authorities in Gradiska haven't responded to his call. Dragana Ilic, deputy mayor of Gradiska, said that the property owners gave permission for the mural of Mladic to be painted and that they were not obliged to seek consent of the city authorities.
This is why the mayor and the city administration cannot request the removal of the mural as it is not the town's property, Ilic explained. "There have been no citizens' initiatives to remove the mural in question," she added.
Before the end of his term, Inzko visited several towns in Republika Srpska where murals of war criminals had been painted and issued an open letter expressing his shock and surprise.
"I read on one Bosnian website that Ratko Mladic had been given a 25-metre-long mural in Foca. I could not believe it!" Inzko wrote.
He said that "glorification of war criminals in unacceptable in the civilised world and there should be consequences".
BIRN contacted the five municipalities that Inzko urged to remove murals, as well as three others where it is known that there are also street artworks praising war criminals. Only Gradiska and one other municipality, Kalinovik, responded.
Like the authorities in Gradiska, the mayor of Kalinovik, Radomir Sladoje, insisted that he had no authority to remove the large...


text:
This foaming and non-stripping pre-cleanser removes product buildup from your scalp while also prepping it for styling. Thanks to its direct-to-scalp applicator, this formula is easy to apply to protective styles.
For use on dry hair. Using the directional nozzle, apply to scalp in one-fourth-inch partings. Massage into scalp before adding water. Adding water will fully activate the foam. Emulsify for one minute, then let sit for one minute more. Rinse out and follow with your preferred Mizani shampoo and conditioner.
Glycerin, Aqua/Water, Sodium Laureth Sulfate, Propylene Glycol, Coco-Betaine, PEG-7 Glyceryl Cocoate, Mel/Honey, Parfum/Fragrance, Sodium Chloride, PEG-55 Propylene Glycol Oleate, Sodium Benzoate, Polyquaternium-7, Salicylic Acid, Hexyl Cinnamal, Cannabis Sativa Seed Oil, Mentha Piperita Oil/Peppermint Oil, Linalool, Menthol, Melaleuca Alternifolia Leaf Oil/Tea Tree Leaf Oil, Limonene.

domain: chemistry


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Articles Tagged ‘FTWA’
We had an interior shot of one of the 15 FTWAs sent in to this week’s competition, which has all the arches from the last two weeks of route launches (because we were in Krakow last week). Find out which airport sent it and whether the all-important exterior shot was good enough to win.
Vienna’s triumphant fire crew celebrates last week’s win for the Air India inaugural – the Austrian capital’s first ever victory in the competition. 10 airports vie for honours in this week’s Arch of Triumph battle.
Over 4,500 votes were cast to determine who the world thinks performed the best FTWA to mark the start of S16 ‒ with Cluj-Napoca’s firefighters coming out on top. This means that the Romanian airport has won both competitions this week, clearly mobilising its local fans and followers to bring home the win.
Splash the route launch cash! The world’s airport firefighters have been busy with their ceremonial hosing down of inaugural aircraft this week, to bring you our twice-yearly public vote for the Arch of Triumph. So while the hoses are drying in the station, get the crews voting to be the best of the best.
The busiest week for 2016 so far with 11 FTWAs entered; Karlsruhe/Baden-Baden poses with its winner’s certificate
In the week before the start of S16, the Arch of Triumph competition has its busiest seven days so far, with 11 entries. With participants from Australasia, Asia, North and South America, Caribbean as well as Europe, we are truly global this week.
A German airport won the 2014 Arch of Triumph competition, when Munich Airport produced the highest score (38.9) of the entire contest for its welcome of United Airlines’ Houston Intercontinental service. Find out whether this week’s German entry can live up to a nation’s lofty expectations.
Sending the anna.aero judges a selection of Arch of Triumph images is always going to work out well, as we always choose the best of the bunch for the competition. That’s what this week’s winner did. Now it can LUXuriate in its glory over the other six competing airports from around the world.
Dubai Airport has already racked up two wins so far in the 2016 Arch of Triumph competition – impressive statistics indeed. Find out here all of the vital numbers from this week’s entrants, as well as the tally in the inter-regional battle.
World’s busiest international airport makes it onto Arch of Triumph leader board for the second time in 2016
A football philosopher once said: “A win is a win is a win.” Wise words that also apply in the Arch of Triumph competition – particularly when there are only two entrants. And just like football…FTWAs are a game of two halves.
It’s Asia versus Europe this week as just two entries battle it out to become Week Six winner of the Arch of Triumph competition in 2016. Find out which of the dynamic FTWA duo has achieved the highest score and brought home the win for their airport.
The airport serving Middle Earth joins Christchurch to become only the second airport in New Zealand to have an Arch of Triumph certificate on its fire station walls. Find out which of this week’s three worldwide entries has racked up enough points to deliver the win for their airport.
At the fifth attempt, Montpellier Airport finally basks in the warm glow of its first ever Arch of Triumph win. Find out which of this week’s six entries – all from Asia or Australasia – has done the business this week and secured legend status for their airport.