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ICT Cabinet Secretary Eliud Owalo has told the Senate that the Government is currently reviewing laws governing cybersecurity and the ICT sector to align them with emerging realities.
Owalo told the Senate ICT Committee sitting in Nairobi that the most notable are the Kenya Information and Communications Act, 1998, and the development of subsidiary legislation for the Computer Misuse and Cybercrime Act.
He said that the ongoing legal reform will where necessary facilitate the enhancement of penalties against cybercrime, including online child sexual exploitation.
“An inter-ministerial and inter-agency committee is spearheading this exercise, the key players involved besides the Ministry of Information and Communications Authority are the Ministry of Interior and the Office of the Director of Public Prosecution, among others,” said Owalo.
The CS told the Committee chaired by Trans Nzoia Senator Allan Chesang that the Government through the Communication Authority has executed two national child online protection campaigns.
The first phase of this campaign dubbed 'Be the Cop' was launched in 2015 to sensitize parents, guardians and teachers on the dangers that exist online and to encourage responsible internet usage, the campaign also sought to trigger other child online protection initiatives in Kenya.
Owalo said the campaign involved several stakeholders including the Department of Children Services, The Cradle, Kenya Girl Guides Association, Kenya Scouts Association, Kenya Association of Professional Counsellors, UNICEF, Google Kenya, Plan International, Terre de Hommes, Childline Kenya, GSMA and mobile service providers Telkom, Airtel, Safaricom and AFRALTI.
The CS told the committee that the second phase of the child online protection campaign carried out in September 2021 was geared towards the implementation of strategic activities that would have a long-term impact on children.
“Through this campaign, the Government, through the Communication Authority executed a three-month media and social media campaign to engage and educate children and the youth on child online protection and safety issues,” said Owalo.
He said that the Government has continued to reinforce public awareness on child online safety through various annual outreach activities, including the Safer Internet Day, Kenya National Music Festival (Annual Event), Kenya National Drama Festival (Annual Event), County ICT Consumer forums (Kikao Kikuu), TV and radio talk shows, Vocational Bible Study Programmes held at various churches.
Owalo noted that through CA, the government leads the national commemoration of the October Cyber Security Awareness Month (OCSAM), which is a collaborative effort that brings together public and private sectors with the goal of raising awareness of cyber safety through empowering consumers with the knowledge, skills and values to safeguard themselves online.
The CA has commemorated OCSAM for the past four consecutive years during which the Government has developed and implemented various initiatives that are geared towards enhancing collective cyber-readiness and resilience, targeting various audiences including professionals, students, children, parents and guardians, as well as the elderly and youth.
“Cybersecurity awareness creation and capacity building are key strategic pillars toward enhancing the collective cyber readiness and resilience and thus support the realisation of Kenya's digital transformation agenda,” said Owalo.
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The government, through the National KE-CIRT/CC, leads national cybersecurity awareness creation, and capacity-building initiatives and issues periodic advisories on emerging cyber concerns through press statements and cybersecurity best practice guides.
Owalo said that the CA engages with schools to provide cyber awareness to students, teachers and parents on emerging cybersecurity concerns such as cyberbullying, child online exploitation and online fraud amongst others.
The Authority this year has held a series of cyber-awareness and cyber capacity-building initiatives in the run-up to OCSAM 2023, geared towards enhancing collective cyber hygiene with these activities including a cybersecurity bootcamp and cybersecurity capacity-building initiatives.
“Through the digital forensics lab, the government, through the National KE-CIRT in collaboration with law enforcement agencies, investigates various cybercrimes, including child online sexual exploitation and abuse, the National KE-CIRT hosts a state-of-the-art digital forensics lab that is used to facilitate the investigation and prosecution of cybercrimes,” said Owalo.

domain: law


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Cesc fabregas dating daniela Xdating nudes
Father once again ❤ couldnt be prouder and happier thank u baby @daniellasemaan7 for bringing another angel to the family and being so brave ❤❤ Padre por segunda vez ❤ no podria ser más feliz gracias mi amor @daniellasemaan7 por traernos a este angel a la familia y por ser una mujer tan fuerte ❤❤ #Capri Fabregas Semaan ☺ A photo posted by Cesc Fàbregas (@cescf4bregas) on "I have very excited to start my adventure at Chelsea and I am here to keep winning titles."Fabregas met Semaan in a Japanese restaurant while she was still married to her property tycoon ex-husband – Elie Taktouk.
She and the property tycoon went on to endure a bitter divorce battle.
“Had [the judge] known that, the outcome might well have been a different one." On appeal Mr Lyon argued there were several other interested parties who were willing to pay much more but have been unable to view the flat while she is in it.
"There's credible prima facie evidence that this property can sell, and sell quickly, for a sum in the region of £7.5 million," Mr Lyon said.
Synopsis: Daniella Semaan is best known for her relation with Spanish and Chelsea F. Early Life: Daniella Semaan was born in Lebanon on June 04, 1975.
Semaan spent most of her childhood in Lebanon along with her siblings.
Besides her relationship with a star footballer, she has been featured as a cover model in several fashion magazines.
"It is rather extraordinary that, less than three weeks after judgment was handed down, through his company in Barcelona he was offering to purchase (the flat).
"What would happen is she would remain in occupation with £1.4 million in the bank, which was not the intention of Mr Justice Coleridge.” Mr Lyon said it was “quite clear” from the judgment that the court had deemed Fabregas a wealthy boyfriend who provided Miss Semaan with funds when she was in Barcelona, paid for taxis, flights and expensive presents.
Semaan gave birth to her first child Maria in 2001 and her second child was born in 2004.
After her divorce with Taktouk, Daniella Semaan has been in a relation with Fabregas since 2011.

domain: celebrity


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Jobst RideBike! book and Fanatyk Spokes are here!
This is a limited reprinting of the 3rd Edition of Jobst Brandt’s classic work, a necessary reference for any aspiring wheel builder and a handsome addition to any cycling lover's library.
Jobst's pioneering research has much to do with the renaissance of wheel design now underway. Back in 1975, when his serious inquiry began, well-built wheels were all too rare and so was our understanding of the physics of this remarkable structure. Leading a motley crew of co-contributing fellow investigators, Jobst fleshed out the true nature of the tensioned wheel and recorded the details for all of us.
It took a fanatical rider and gifted engineer (Jobst), an academic community (Stanford), an energetic and curious cycling scene (Bay Area), for such a breakthrough work. It was Jobst’s intention that the book would not easily go out of relevance. Hence, a minimum reference to brands, innovations, trends, etc. Just the eternal basics of tensioned wheels. As relevant today as 40 years ago.
Collections: Useful Bits

domain: hobby


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We aren’t leaving Tanzania just yet. Sometimes you have to visit a place just because the name beckons. You can’t deny the allure of Timbuktu, Samarkand, Rapanui. That’s why we’re heading for Zanzibar.
It’s Jack’s birthday. Two years ago he celebrated by having knee replacement surgery. We’re pretty sure this is going to be better.
We took our last photo of Mount Kilimanjaro, boarded a small plane and flew the 250-ish miles to Stone Town. We were sad to be leaving the game parks, but eager for a new destination.
We made no plans beyond booking a hotel near the sea for a few days with the understanding that if we felt the need to go further afield, we’d move on later in the week..
Our style of travel has evolved over the years. We no longer do much planning. We like to just show up and figure it out when we get there. I did look up “Top 10 Things to Do in Zanzibar.” Maybe later.
Stone Town in Zanzibar is one of those cultural crossroads, a UNESCO World Heritage site that commemorates the ancient Swahili village that became a trading post for Chinese, Arab, Asian, Indian and European merchants. Zanzibar is called the Spice Island, although it’s also known for trade in ivory and even more so, enslaved people.
The people, architecture and cuisine reflect that history. Jack and I love this kind of place, where the mix of influences is right there in front of you, in the faces, the buildings, the restaurant menus. Just wandering the narrow streets on our own without a guidebook is enjoyable.
Stone Town has a more recent claim to fame as the birthplace of Freddie Mercury. We skipped the museum but I did take a photo, right before two guys reminded us that Stone Town still has one foot firmly in the past.
New or restored buildings share space with old colonial era ones that the town refuses to give up on.
We dined at local eateries where we got friendly advice on what to order.
In the late afternoons we walked to the beach. I think barring the plastic buckets and jugs in the foreground, not much has changed here in centuries.

domain: travel


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Cantucci: here’s a great recipe for a typical Italian cookies, with our Sara’s Cantucci Abruzzesi version. Originally from Tuscany, (also called “Prato biscuits”), their peculiarity is the double cooking: “bis-cuit”.… Sweet Recipe: Sara’s Cantucci Abruzzesi
Category: Recipe Testing
You should try this…!
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domain: food


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monsters and mysteries in america:Appalachia
The dark hallows and dense forests of Appalachia harbor strange mysteries. Local residents will recount their encounters with the terrifying Sheepsquatch, the dark winged-creature, Mothman, and aliens from space.
Yes! I want them to riff on Sheepsquatch!
Zachary Cheatwood commented
the other one was deleted here is the ep

domain: geography


Solar Energy Bundaberg - invest in your home, business or land.
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You have come to the right place - Sunvest provide solar power with a difference. Our service is peerless, and we are experienced in - and skilled at – the installation of solar power systems. Based in Bundaberg, Queensland, Sunvest provides solar power systems to responsibly power your home – and save you money. If you need solar power in Bundaberg call Sunvest, you local solar power business.
With Solar Power Bundaberg can say goodbye to electricity bills
From the very first day your solar energy system is installed you can have extra money in your pocket each and every day. We have a variety of packages available that clearly demonstrate how solar panels (installed by Bundaberg-based solar panel installers) can make this can happen. Check out our Bronze, Silver and Gold solar energy packages under our Residential services section for more information, or head into our HQ and chat to Sunvest, the solar power experts in Bundaberg.


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Half way through October and well into the season, featuring wolf songs.
8:58pmLazerbeak “Let It Go”
from Legend Recognize Legend Album (Doomtree Records 2010)
10:48pmWolf & Cub “Spider'S Web”
from Science and Sorcery Album (Remote Control Records, under exclusive license to Last Gang Records

domain: artistic


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Orcs Must Die! Unchained: CB Phase 2 Begins on March 24th
Mark your calendars and clear your week. March 24th will see the triumphant release of Closed Beta Phase 2 for Orcs Must Die Unchained. The development team has been information players about all of the exciting new changes for weeks, and soon you’ll get to try them our for yourself. Tons of changes include Gameplay 2.0, New Traps, UI, a new orc map and more.
For those that want an early sneek peak, Phase 2 for Orcs Must Die Unchained is currently playable at PAX East this weekend in Boston.
For more info, visit https://www.orcsmustdie.com/
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- HotS - Varian Hero Review
- Varian was never one to back down from a difficult task or shy away from the front lines.

domain: game


text:
Cannot get Howe Sound location id in module mod_sp_weather. Please also make sure that you have inserted city name.
Bowen Island Marina Gibsons Landing Harbour Authority Gibsons Marina & Fuel
Lions Bay Marina Sewell's Marina Squamish Harbour Authority
Sunset Marina Ltd. Thunderbird Marina Union Steamship Co. Marina
Not to be used for navigation


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Cyanidin-3-glucoside suppresses the progression of lung adenocarcinoma by downregulating TP53I3 and inhibiting PI3K/AKT/mTOR pathway
World Journal of Surgical Oncology volume 19, Article number: 232 (2021)
The aim of this study is to unravel the role of Cyanidin-3-glucoside (C3G) and its potential mechanisms in lung adenocarcinoma (LUAD).
The cell clones, proliferation, apoptosis, migration, and invasion in H1299 and A549 cells were determined by colony formation assay, 5-ethynyl-20 deoxyuridine (EdU) assay, flow cytometry, and transwell assay, respectively. The expression of p53-induced gene 3 (TP53I3) was assessed and the prognostic values of TP53I3 in LUAD via the dataset from the Cancer Genome Atlas (TCGA). In addition, the mRNA and protein expressions were detected by quantitative real-time PCR (qRT-PCR) and western blot.
C3G inhibited the proliferation, migration, and invasion of, and also promoted the apoptosis in H1299 and A549 cells. The database of TCGA showed TP53I3 was highly expressed in LUAD tissues and correlated with the poor prognosis of LUAD patients. Moreover, we also found that C3G inhibited the proliferation, migration and invasion, and promoted apoptosis in H1299 and A549 cells by downregulating TP53I3. Additionally, C3G could inhibit the activation of phosphatidylinositol 3′-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway in H1299 and A549 cells by downregulating TP53I3.
This study demonstrated that C3G could inhibit the proliferation, migration and invasion, and also facilitate the apoptosis through downregulating TP53I3 and inhibiting PI3K/AKT/mTOR pathway in LUAD.
Lung cancer is the second most frequent cancer in both men and women, and it is considered to be one of the major causes for cancer-related death worldwide [1, 2]. Non-small cell lung cancer (NSCLC) accounts for about 85% of diagnosed lung cancer cases [1, 3, 4]. Moreover, NSCLC can be divided into lung adenocarcinoma (LUAD), lung squamous cell carcinoma, and large cell cancer . Despite the recent advances in surgery, radiotherapy, and chemotherapy, the 5-year survival rates remain dismal . Unfortunately, most patients are usually diagnosed at an advanced stage . Therefore, exploring new drugs and therapeutic targets for LUAD is urgently needed.
Cyanidin-3-glucoside (C3G), one of the most abundant anthocyanidin in a wide variety of fruits and vegetables, has been reported to have multiple beneficial effects for cardiovascular diseases, diabetes, and inflammation [8,9,10]. In recent years, some studies have indicated that C3G can exert inhibitory effects in a variety of cancers. For example, C3G could attenuate the migratory and invasive capacity in breast cancer cells by regulating Sirt1 expression . Moreover, the study by Chen et al.  has reported that C3G can exhibit an inhibitory effect on the migration and invasion of lung cancer cells. However, the potential mechanism of C3G on LUAD has not been widely explored.
The p53-induced gene 3 (TP53I3 or PIG3), one of the p53-induced genes, is involved in both the p53-mediated apoptosis and DNA damage response [13,14,15]. TP53I3 is reported to play important roles in apoptosis via reactive oxygen species generation and oxidative stress induction . In other words, TP53I3 alone cannot directly induce apoptosis unless it cooperates with a set of simultaneously activated pro-apoptotic genes induced by reactive oxygen species . However, the roles of TP53I3 alone on apoptosis are rarely explored in previous studies, and investigated in this study. In recent years, the function of TP53I3 in tumors has attracted much attention due to its relationship with apoptosis and DNA damage response. Xu et al.  have reported that TP53I3 could facilitate the growth of papillary thyroid cancer. A study by Park et al. has confirmed that the downregulation of TP53I3 can inhibit the migration and invasion of colon cancer cells . Gu et al.  also suggest that TP53I3 can promote cell migration and invasion in LUAD. UALCAN database suggests that TP53I3 was highly expressed in LUAD patients’ tissues, and LUAD patients with higher TP53I3 levels had a worse prognosis, which confirms the study of Gu et al. . Additionally, the bioinformatics analysis shows that C3G is associated with TP53I3 in LUAD. Therefore, whether C3G exerts effects on LUAD cells by regulating TP53I3 was investigated.
In the present study, the findings revealed that C3G could inhibit the proliferation, migration, and invasion, and also facilitate the apoptosis by downregulating TP53I3 in LUAD. Moreover, C3G could inhibit the activation of PI3K/AKT/mTOR pathway by downregulating TP53I3 in LUAD cells. The findings of this study suggest that C3G is a potential compound for LUAD treatment.
Cell culture and reagents
Human LUAD cell lines (H1299 and A549) and normal lung epithelial cell line BEAS-2B were supplied by American Type Culture Collection (ATCC, USA). All cell lines were cultured at 37 °C in a humidified atmosphere containing 5% CO2 in Dulbecco’s modified Eagle medium (DMEM, Invitrogen, USA) supplemented with 10% fetal bovine serum (FBS, Wisent, Canada). C3G (98.0% purity) was obtained from Tokiwa Phytochemical Co., Ltd., Japan, and dissolved in dimethyl sulfoxide (DMSO, Sigma-Aldrich, USA) for the subsequent assays.
Small interfering RNA targeting TP53I3 (si-TP53I3) and siRNA negative control (si-NC) were purchased from GenePharma (Shanghai, China). In brief, H1299 and A549 cells (6 × 105/well) were planted into a 6-well plate and then cultured with or without C3G for 24 h. Then, the cells were transfected with si-TP53I3 or si-NC by using Lipofectamine 3000 (Invitrogen, USA) following the manufacturer’s instructions. Forty-eight hours later, the cells were harvested for the subsequent experiments.
Cell counting kit-8 assay
Cell viability was assessed with Cell Counting Kit-8 (CCK-8, Dojindo Molecular Technologies, USA). Simply, H1299, A549, and BEAS-2B cells (3 × 103/well) were seeded in 96-well plates and then exposed to different concentrations of C3G (0, 5, 10, 20, 40, and 80 μM) for 24 h. After that, the cells were harvested, and then CCK-8 solution (10 μL) was added to each well. Finally, the absorbance at 450 nm was tested by a microplate reader (model 680, Bio-Rad, USA).
Colony formation assay
H1299 and A549 cells were exposed to C3G (10, 20, and 40 μM) for 24 h. Afterwards, they were seeded in 12-well plates in triplicate and cultured for 2 weeks at 37 °C in a 5% CO2 incubator. Culture medium was replaced every 3 days. Then, 4% paraformaldehyde was applied to fix the colonies. Finally, the cell colonies were stained with 0.1% crystal violet (Beyotime, Jiangsu, China) for 20 min and counted under a light microscopy.
5-Ethynyl-20-deoxyuridine (EdU) assay
The proliferation of H1299 and A549 cells was determined by using the EdU incorporation assay kit (Ribobio, Guangzhou, China). Simply, the cells were cultured with EdU solution for 2 h and then fixed with PBS containing 4% paraformaldehyde. Subsequently, the nucleus was stained with 4′,6-diamidino-2-phenylindole (DAPI, Beyotime Biotechnology, Shanghai, China). Finally, the cells were visualized with a fluorescence microscopy.
Flow cytometry analyses
To investigate cell apoptosis, flow cytometry was carried out. The cells were resuspended and stained with Annexin V-FITC for 5 min in dark at 4 °C under the construction of the Annexin V-propidium iodide (PI) kit (Beyotime Biotechnology, Shanghai, China). Subsequently, the cells were stained with propidium iodide (PI) for 5 min, and finally analyzed using a FACScan flow cytometer (BD Biosciences, USA).
Cell invasion assay was carried out by using a Corning Polycarbonate Membrane Insert transwell chamber (8-μm pore size, Corning, USA) coated with Matrigel (BD Bioscience, USA) and cell migration assay was performed in a similar method without Matrigel. The cells with 200 μL FBS-free medium were placed into the upper chamber and 600 μL medium containing 20% FBS was added into the lower chamber. After a 24-h incubation, the non-migrated or non-invaded cells were removed with a cotton swab, while the migrated or invaded cells were fixed with 4% paraformaldehyde for 30 min and stained with 0.5% crystal violet for 30 min. Finally, the migrated or invaded cell numbers were counted under microscope.
Total RNA was extracted by using TRIzol Reagent (Thermo Fisher, USA), followed by reverse-transcription for complementary DNA (cDNA) synthesis with the One Step PrimeScript cDNA Synthesis Kit (Takara, Japan). Then, qRT-PCR analysis was performed on ABI 7500 system (Applied Biosystem, USA) with the SYBR Premix Ex Taq II kit (Takara, Japan). GAPDH was considered as the endogenous control. And the relative expression levels were calculated by the 2−ΔΔCt method. All primers were designed by GenePharma (Shanghai, China) as follows: TP53I3 F: 5′-CCATGCAGGACTGAGTGGTG-3′, R: 5′-CTGCTCCAAGCTTTTCTGCC-3′; GAPDH F: 5′-CATGAGAAGTATGACAACAGCCT-3′, R: 5′-AGTCCTTCCACGATACCAAAGT-3′.
Total proteins from H1299 and A549 cells were homogenized by using RIPA lysis buffer (Solarbio, Beijing, China). The protein sample (50 μg) was separated through the assay of sodium dodecyl sulfate–polyacrylamide gel electrophoresis and then transferred onto the polyvinylidene difluoride membrane (Millipore, USA). After blocked with 5% skim milk powder, the membrane was incubated with the primary antibodies (Bax, 1:1000, no. 50599–2-Ig; Bcl-2, 1:1000, no. 12789–1-AP; E-cadherin, 1:1000, no. 20874–1-AP; N-cadherin, 1:1000, no. 22018–1-AP; Vimentin, 1:500, no. 10366–1-AP; MMP-2, 1:1000, no. 10373–2-AP; MMP-9, 1:1000, no. 10375–2-AP; PI3K, 1:1000, no. 20584–1-AP; AKT, 1:1000, no. 10176–2-AP; mTOR, 1:500, no. 20657–1-AP; GAPDH, 1:1000, no. 10494–1-AP, Proteintech, USA. TP53I3, 1:500, no. ab96819; p-PI3K, 1:300, no. ab182651; p-AKT, 1:500, no. ab38449; p-mTOR, 1:500, no. ab109268, Abcam, USA) at 4 °C overnight. After that, the membrane was washed with Tris-buffered saline Tween-20 (TBST) and incubated with the secondary antibody (no. 7076, Cell Signal, USA) for 1 h. An enhanced chemiluminescence kit (ECL, Thermo Fisher, USA) was applied to determine the immunoreactive bands.
The possible target genes of C3G were predicted though retrieving the Bioinformatics Analysis Tool for Molecular mechANism of Traditional Chinese Medicine (BATMAN-TCM, http://bionet.ncpsb.org.cn/batman-tcm/index.php/Home/Index/index), the genes with inference score > 20 in the Comparative Toxicogenomics Database (CTD) (http://ctdbase.org/) and the LUAD relative genes in the DisGeNET database (https://www.disgenet.org/). Then, the genes were intersected using Venn analysis (http://bioinformatics.psb.ugent.be/webtools/Venn/) . Moreover, the differential expression of TP53I3 in LUAD patients tissues and the relative prognosis curve of LUAD patients were analyzed by the UALCAN (http://ualcan.path.uab.edu/) . The database used in UALCAN was obtained from the Cancer Genome Atlas (TCGA) database (https://cancergenome.nih.gov/). The criteria for patients dividing into the two groups on prognosis curve is the Quartile.
For statistical analysis, the GraphPad Prism 8.0 was used. The data are presented as mean ± standard deviation (SD). Comparisons among multiple groups were carried out by one-way analysis of variance (ANOVA) followed by a Bonferroni post hoc test. P < 0.05 was chosen as a statistically significant result. All experiments were performed in triplicate.
C3G suppresses the viability of H1299 and A549 cells
The effect of C3G on the viability of H1299, A549, and BEAS-2B cells was assessed by using the CCK-8 assay. As seen in Fig. 1A and B, cell viability was markedly reduced in C3G 10-, 20-, 40-, and 80-μM groups in both H1299 and A549 cells and in a dose-dependent manner (P < 0.05, P < 0.01). Moreover, only C3G 80 μM had little effect on normal lung epithelial cell BEAS-2B (P < 0.05, Fig. 1C). These results indicated that C3G may inhibit LUAD cell growth. In the subsequent experiments, C3G (10, 20, and 40 μM) was selected to further explore C3G effect on H1299 and A549 cell.
C3G inhibits the proliferation and promotes the apoptosis in H1299 and A549 cells
The results of colony formation assay (Fig. 2A) and EdU assay (Fig. 2B) showed that C3G (10, 20, and 40 μM) notably inhibited the proliferation of H1299 and A549 (P < 0.01). As Fig. 2C showed, when compared with Control group, C3G (10, 20, and 40 μM) markedly facilitated the apoptosis of H1299 and A549 cells (P < 0.01). In addition, the function of C3G on the expressions of Bax and Bcl-2 was also investigated by western blot (Fig. 2D). The data revealed that the treatment of C3G (10, 20, and 40 μM) significantly increased the expression of Bax in H1299 and A549 cells (P < 0.01), but decreased Bcl-2 expression (P < 0.01). Altogether, the data demonstrated that C3G could inhibit the proliferation and promote the apoptosis in H1299 and A549 cells.
C3G inhibits the migration and invasion in H1299 and A549 cells
The migration and invasion in H1299 and A549 cells were assessed by transwell assay (Fig. 3A and B). The results showed that the treatment of C3G (10, 20, and 40 μM) significantly repressed the migration and invasion of H1299 and A549 cells (P < 0.05, P < 0.01) when compared with Control group. In addition, western blot results (Fig. 3C) demonstrated that C3G (10, 20, and 40 μM) markedly elevated E-cadherin expression level in H1299 and A549 cells (P < 0.01), but dramatically reduced the expressions of N-cadherin, Vimentin, MMP-2, and MMP-9 (P < 0.05, P < 0.01). All accumulating results indicated that C3G could inhibit the migration and invasion in H1299 and A549 cells.
C3G suppresses the expression of TP53I3 in H1299 and A549 cells
Based on the database of BATMAN, CTD, and DisGeNET, C3G was closely associated with six genes (TP53I3, CRYZ, NQO1, SOD1, SOD2, and CBR1) (Fig. 4A). In addition, the database of TCGA showed TP53I3 was highly expressed in LUAD tissues (Fig. 4B) and related to the prognosis of LUAD patients (Fig. 4C). Moreover, the upregulation of TP53I3 was also confirmed in LUAD cells (P < 0.01) (Fig. 4D). Thus, the relationship between C3G and TP53I3 was further investigated. As shown in Fig. 4E, qRT-PCR was used to verify the transfection efficiency. The results of qRT-PCR and western blot showed that the treatment of C3G (10, 20, and 40 μM) significantly inhibited TP53I3 expression in both H1299 and A549 cells (P < 0.01) (Fig. 4F and G). These results demonstrated that C3G could suppress the expression of TP53I3 in H1299 and A549 cells.
C3G inhibits the proliferation, migration, and invasion, and promotes apoptosis in H1299 and A549 cells by downregulating TP53I3
As shown in Fig. 5A, the cell colonies of H1299 and A549 cells were significantly decreased in the si-TP53I3 and C3G + si-NC groups relative to the si-NC group (P < 0.01). When compared with the si-TP53I3 and C3G + si-NC groups, the cell colonies were markedly reduced in the C3G + si-TP53I3 group (P < 0.01) (Fig. 5A). The results of Fig, 5B showed that the apoptosis of H1299 and A549 cells was notably increased in the si-TP53I3 and C3G + si-NC groups compared with the si-NC group (P < 0.01). Meanwhile, in comparison with the si-TP53I3 and C3G + si-NC groups, cell apoptosis was significantly elevated in the C3G + si-TP53I3 group (P < 0.01) (Fig. 5B). Additionally, transwell assay confirmed that the migration and invasion of H1299 and A549 cells were significantly increased in the si-NC group relative to the si-TP53I3 and C3G + si-NC groups (P < 0.01), but dramatically decreased in the C3G + TP53I3 group (P < 0.01) (Fig. 5C and D). These results indicated that C3G could inhibit the proliferation, migration and invasion, and promote apoptosis in H1299 and A549 cells through downregulating TP53I3.
C3G inhibits the activation of the PI3K/AKT/mTOR pathway in H1299 and A549 cells by downregulating TP53I3
As seen in Fig. 6A, the gene set enrichment analysis (GSEA) showed that the high expression of TP53I3 was positively associated with the “PI3K/AKT/mTOR pathway” in LUAD. Therefore, whether C3G could regulate PI3K/AKT/mTOR pathway via downregulating TP53I3 was further assessed. Western blot results showed that the expressions of p-PI3K, p-AKT, and p-mTOR in H1299 and A549 cells were markedly lower in the si-TP53I3 and C3G + si-NC groups than that in the si-NC group (P < 0.01) (Fig. 6B). On the contrary, when compared with the si-TP53I3 and C3G + si-NC groups, the expressions of p-PI3K, p-AKT, and p-mTOR in H1299 and A549 cells were significantly reduced in the C3G + TP53I3 group (P < 0.01) (Fig. 6B). All these results confirmed that C3G could inhibit the activation of the PI3K/AKT/mTOR pathway in H1299 and A549 cells by downregulating TP53I3.
The mortality of lung cancer ranks first among all kinds of malignant tumors . Despite encouraging progress with new drugs in LUAD, the prognosis of patients with advanced cancer is still poor. Therefore, the exploration of novel drugs and therapeutic targets for LUAD treatment is urgently needed. In this study, C3G could inhibit the proliferation, migration, and invasion, and also facilitate the apoptosis through downregulating TP53I3 and inhibiting the PI3K/AKT/mTOR pathway in LUAD.
A growing number of evidence has confirmed that C3G plays an important role in a variety of cancers. For instance, C3G is able to suppress tumor cell growth, migration, and invasion, and induce apoptosis in breast cancer . Hosseini et al. have indicated that C3G has a significantly pro-apoptotic effect in the glioblastoma cells . In addition, the study by Chen et al.  has reported that C3G can exhibit an inhibitory effect on the migration and invasion of lung cancer cells. In the present study, C3G was also confirmed to inhibit the proliferation, migration, and invasion, and promote the apoptosis of LUAD cells. The epithelial–mesenchymal transition (EMT) status plays a crucial role in cancer cell metastasis and invasion . EMT is accompanied by the downregulation of epithelial markers, such as E-cadherin, and the upregulation of mesenchymal markers such as N-cadherin and Vimentin [25, 26]. The results confirmed that C3G significantly increased E-cadherin expression, but decreased the expression of N-cadherin and Vimentin, suggesting that C3G could inhibit LUAD cell migration and invasion by repressing EMT process.
In recent years, several studies have suggested that TP53I3 may play an important role in various types of cancers. TP53I3 is reported to be highly expressed in papillary thyroid carcinoma and plays oncogenic roles through the activation of the PI3K/Akt/PTEN pathway . The database of TCGA showed TP53I3 was highly expressed in LUAD tissues and correlated with the poor prognosis of LUAD patients, further confirming that TP53I3 may be an oncogenic gene in LUAD. However, whether C3G is able to regulate TP53I3 in LUAD remains unknown. Thus, the relationship between C3G and its target genes was further explored via the database of BATMAN, CTD, and DisGeNET. The results showed that C3G was closely associated with six genes (TP53I3, CRYZ, NQO1, SOD1, SOD2, and CBR1). Moreover, C3G was found to significantly inhibit the expression of TP53I3 in H1299 and A549 cells. More and more studies have shown that TP53I3 can exhibit important effects in cancer cell biological behaviors. For example, TP53I3 overexpression could elevate the colony formation, migration, and invasion ability of colon cancer cells . Xu et al. have reported that TP53I3 could facilitate the growth of papillary thyroid cancer through activating the PI3K/AKT/PTEN pathway . Gu et al. have confirmed that TP53I3 could promote cell migration and invasion in lung adenocarcinoma . In the present study, TP53I3 silencing significantly inhibited the proliferation, migration, and invasion, and promoted apoptosis in LUAD cells. Additionally, C3G could also inhibit the proliferation, migration, and invasion, and promote apoptosis in H1299 and A549 cells through downregulating TP53I3.
Emerging evidence has suggested that the PI3K/AKT/mTOR pathway is a signal transduction pathway involved in the modulation of multiple cellular functions including cell survival, proliferation, cell cycle, apoptosis, autophagy, differentiation, migration, and invasion [27,28,29]. In recent years, more and more researches have reported that the PI3K/AKT/mTOR pathway is aberrantly activated in NSCLC and associated with the progression of NSCLC [30,31,32,33]. A study by Xu et al.  has reported that TP53I3 could play oncogenic roles in papillary thyroid cancer through the activation of the PI3K/AKT/PTEN pathway. GSEA data suggested that the high expression of TP53I3 was positively associated with the “PI3K/AKT/mTOR pathway” in LUAD. In the present study, C3G could inhibit the activation of PI3K/AKT/mTOR pathway by downregulating TP53I3 in LUAD cells.
In conclusion, C3G could inhibit the proliferation, migration, and invasion, and also facilitate the apoptosis through downregulating TP53I3 and inhibiting PI3K/AKT/mTOR pathway in LUAD. These findings implied that C3G may be an effective therapeutic drug for LUAD treatment.
Availability of data and materials
The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.
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Chen, X., Zhang, W. & Xu, X. Cyanidin-3-glucoside suppresses the progression of lung adenocarcinoma by downregulating TP53I3 and inhibiting PI3K/AKT/mTOR pathway. World J Surg Onc 19, 232 (2021). https://doi.org/10.1186/s12957-021-02339-7

domain: biology


The car is an absolute blast to drive! Handles better than any of the 30 or so I've ever driven. The mid-engine/rear wheel drive layout along with the rack and pinion steering allow you to really feel the road, and push the car to the limits.
The engine is easy to work on, as is most of the car. Anyone of moderate mechanical ability can fix most of the problems they're likely to encounter.
Most components that failed or needed to be replaced were the 20 year old originals. No parts that I've replaced have failed a second time. Parts are fairly inexpensive, especially those shared with other GM cars.
The car is far more reliable than my previous vehicle. It makes for an excellent daily driver if it is carefully maintained.
My only real complaint is the lack of power from the 2.5L four cylinder "Iron Duke" engine. While the car is quick, it really isn't fast.
I also regret not waiting to find a manual transmission car, although the automatic can still be almost as fun.
You won't find a better car for the price these usually go for!


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HG Drain Hair Unblocker
Hair is the cause of blockages in sink, shower or bath drains in a bathroom. Soap remnants, skin grease and toothpaste that are retained by hair only makes the problem worse.
HG hair unblocker has been strengthened with potassium hydroxide and therefore makes quick work of this problem and completely dissolves the hair and all materials blocked by it.
Box contains 1x200ml bottle + 1x250ml bottle of solution.
View the video below for more information.

domain: chemistry


text:
After a successful Kickstarter, publisher RJ Casey has published a number of new books from his imprint, Yeti Press.Most of these books are by relative newcomers, but a number of them are printed in lavish color. In general, these books have an all-ages vibe to them.
Bird Witch, by Kat Leyh. Leyh collected the first few issues of her minicomics series in this book, and it's an attractive package. This is a well-developed but character-based fantasy story that slowly builds its backstory around the friendship of young witch Nina and her new friend Tali, who is of a race that is part bird. They initially bond over flying (Nina with her broom, Tali with her wings), and Leyh skillfully establishes their friendship and personality types before introducing a conflict in the form of the Bird Witch, an evil being that captures rare types of birds for her own ends. There are chase scenes, reversals, desperate plans and a last-second rescue by a relative. Leyh is definitely of the Kate Beaton school of cartooning in terms of her line, body language and simply-designed characters. Color adds texture and depth to her work, making the world feel real and making Tali all the more exotic because of the rich colors and patterns of her clothing. Later chapters in the book flesh out the world a bit, as Nina is revealed to be the daughter of a forest warden, who protects the magical forest against evil creatures; this is her ambition in life as well. There are bits and pieces of subplots and foreshadowing laid down for future chapters, but the real focus on the book is the way in which Nina and Tali quickly bond and find ways to get into trouble. Despite the extensive use of color, it all feels organic rather than part of an assembly line, like the Amulet books.
Pecos, by RJ Casey and Eric Roesner. This is sixty pages of total nonsense. Casey writes and Roesner draws the adventures of Pecos Bill, a bounty hunter in some crazed, mythical version of the old west that includes a demented, opium-dealing Johnny Appleseed and a Paul Bunyan who is all too intimate with his blue ox. Pecos Bill is one of those "one-upping" kind of characters, where everyone tries to one-up each other by talking about his deeds in the first story. Things are a bit silly and crazy throughout the book, but Casey takes it to the next level in a sequence where Pecos Bill rides one of Paul Bunyan's sperm while he's having sex with Babe the blue ox, then plants explosives inside Babe's body while singing a deranged song to the tune of "Home On The Range". It's a pretty magnificent gross-out gag, one that justifies the tamer and duller jokes earlier in the book, which are fairly disposable. Roesner's exaggerated and grotesque character design does a lot to carry the book, though it's uneven at times and some of his storytelling was difficult to follow at times.
Rose From The Dead, by Andrea Bell. This supernatural romance book feels like something Slave Labor might have published twenty years ago. Bell's character design and use of color are both quite appealing, as she makes the graveyard setting both spooky and funny. It's the story of long-time friends Jack and Roz on Halloween, when Jack is finally determined to tell his whirlwind of a friend that he's in love with her. She can't quite stand still long enough for him to tell her, and all of this is interrupted by skeletal ghosts, one of whom drags Roz into his grave. This is a charming comic, even if the relationship between Jack and Roz isn't exactly novel, and Roz makes for a compelling and clever heroine. The problem with the book is the page design. The panel-to-panel transitions are frequently confusing, as the order of the panels is often unclear. The dialogue and action is frequently vague enough such that it doesn't help clear this up any. Once Bell is able to clean that up a bit, she'll be on her way, because she has the skill to create fast-moving stories with clearly-defined characters and add just the right amount of humor.
Poops Boobs Poo, by Sam Sharpe. This is a killer collection of gags greatly inspired by Ivan Brunetti and the tradition of classic single-gag cartooning in places like The New Yorker. The gags run from the silly (a squirrel talking about how it bothered him just how much he liked nuts), to the edgy and violent (a man holding another man's severed head saying "You tell me this now?" after Moses brought down the ten commandments), to the gross (an amusing fisting joke). There are bad puns, penis size jokes and even a few call-back gags throughout the book. Sharpe has two things on his side: the level of craft in constructing his gags and his draftsmanship. Take the cartoon above, for example. The reader is immediately drawn to the figure in the middle thanks to the way he's positioned on the page. The reader immediately understands that he's a cyclops, that he's unhappy, and that his friend is exuberant after walking out of a film. Searching for context (and going from left to right), the punchline immediately becomes evident thanks to the "Blast-o-rama in 3D" poster. Some of Sharpe's gags had images that existed solely to set up the caption as the punchline, while others used words to set up a funny image as the punchline. He doesn't go quite as far as a Brunetti or a Ryan in most of his gags, in part because he's more about the laugh than the shock, but he's not afraid to get pretty transgressive. Sharpe is a thoughtful, interesting young cartoonist and it was fun to see him cut loose on this kind of gag, almost as an academic exercise.
Well Come, by Erik Nebel. This is a vivid, funny and sometimes gross series of silent cartoons about the interactions of various cartoon shapes. Some are sharply delineated as people, and some are shapes that blend, bleed or outright take over other shapes. Some shapes complete others, making a new mouth or a smile. Other shapes infiltrate or steal parts from other shapes outright. Nebel's use of color is almost psychedelic in its brightness, and the thick, glossy paper allows the color to really soak in on the page. The semi-connected mini-narratives bring to mind certain Lewis Trondheim comics in the way that blobs interact with other blobs in a frequently violent and/or visceral manner. The Team Society League collective's gross comics are another touchstone, though Nebel mostly resists gross-out gags in favor of a gentler sense of humor. The use of color reminds me a bit of Paper Rad a bit, though without the greater weirdness and depth of strange narrative. What you see is what you get on these pages, and Nebel's adherence to following the logic of each gag to its ultimate conclusion works well on page after page. It's the sort of book that you never quite want to end.

domain: library


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Beep Beep..... Reminder!
Hey everyone, I bet you guys saved the date but still I thought I’d go ahead and personally remind you all about the free play for all ages which is this Saturday, January 11 2014 at 10 AM. The play is based on the book Tomas and the Library Lady by Pat Mora and will be held at The Centrum, across from the library. I hope all of you can make it. It shall be uber fun.


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Escrows Poems | Examples of Escrows Poetry
Escrows Poems. Below are examples of poems about escrows. This list of poetry about escrows is made of PoetrySoup member poems. Read short, long, best, famous, and modern examples of escrows poetry. This list of works about escrows is a great resource for examples of escrows poems and show how to write poems about escrows. Definitions may be included where appropriate.
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The Misuse Of NAB Laws ‘Destroyed’ Several Businesses: CJP:
The Misuse Of NAB Laws ‘Destroyed’ Several Businesses: CJP
Islamabad: Chief Justice of Pakistan Umar Ata Bandial on Tuesday said that many people’s businesses were destroyed due to the misuse of National Accountability Bureau (NAB) laws, following the amendments to the National Accountability Ordinance, 1999.
The CJP made the remarks while hearing Pakistan Tehreek-e-Insaf chief Imran Khan’s petition against the recent amendments, wherein the deposed premier’s lawyer Khawaja Harris appeared before the court.
During the hearing, Imran’s counsel maintained that people have to approach the courts when the executive fails to do their job.
Justice Bandial said that the NAB amendment was made by the assembly which was incomplete at the time and highlighted that there was no law about an incomplete assembly’s ability to legislate.
He added that more than half of the National Assembly had boycotted the move because of the “political strategy”
In his remarks, Justice Ejaz-ul-Ahsan said only a few benefited from NAB amendments and that an entire political party was running on “the line” given by a few people. “If you follow the line, it benefits only a few people,” he added.
He maintained that the NAB amendments were approved hastily, without discussion, and that if tweaks in law are made with “ill-intention then one should not proceed” with it.
CJP Bandial remarked that corruption could not end despite court decisions and emphasised that not only corruption, but flaws were also present in the system.
“No attempt was ever made to fix the flaws in the system,” he said, adding that the parliament’s job is to enact and implement laws to improve the system, however, in Punjab a police inspector general is changed after five months and a station house office (SHO) is changed after three months.
“The law is not followed while making executive decisions,” he remarked.
He furthered that the decisions pertaining to Reko Diq and Steel Mills were made by the court with good intentions and that the government could not catch the corruption in the projects due to the weakness of the system.
He stated that many people’s businesses were destroyed due to misuse of NAB law and iterated that corruption should not be pardoned under any circumstances.
The chief justice said that many issues had improved in Pakistan and that the country’s media was “free and presents the truth”.
Justice Mansoor Ali Shah said that Pakistan was 80th on the corruption index ranking prior to the NAB amendments and must have fallen by 100 points post them.
Justice Ijazul Hassan agreed with his statement and said that when the system is collapsing, the judiciary intervenes. He asked under which authority could the court declare the NAB amendments null and void due to a conflict of interest.
Imran’s lawyer Haris said that the term “regulatory capture” is used to refer to legislation made for self-interest.
The CJP asked if the recent NAB amendments would then be called “parliamentary capture”, to which the lawyer stated that parliamentary capture was used in a different context.
A day earlier, the SC had asked the PTI to apprise it of its position on whether keeping the armed forces out of the ambit of the accountability law was a democratic act and how leaving parliament after being elected and seeking decisions through street power served democracy.
Earlier this year, PTI Chairman and former premier Imran Khan had challenged the recent amendments to the National Accountability Ordinance, 1999 in the apex court, arguing that the tweaks would pave the way for public office holders to get away with white-collar crimes.
The PTI chief had filed a constitutional petition under Article 184 (3) through renowned lawyer Khawaja Haris, who was former premier Nawaz Sharif’s counsel in the Panamagate case.
Imran had earlier declared his intention to challenge the amendments in the apex court and had said they were tantamount to “legitimising” corruption.
Supreme Court’s Justice Ijaz ul Ahsan, while listening to the petition against the accountability law amendments, had remarked that the government was trying to get its ‘sins’ forgiven through the tweaks.


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This is an amazing preset pack to create energetic visual effects. Create bright animated strokes, dynamically reveal your logo and text. Includes electric, fire, neon, liquid, magic styles. Using Path Energizer is a great way to quickly create many unique animations with an impressive energy look, it will be great on a footage, cartoon or any other project.
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domain: design


text: Teleo Alley sits close to the heart of the town, and a great deal of traffic streams along the narrow, cobbled road at all times of day. Both sides of the road are packed with small shops and cramped housing. Shouts, laughter and chatter can be heard constantly, although there's a been a darker undercurrent recently. Perhaps they feel the presence of the Dark Lord Flaganroth, or alternatively they just say nasty things about you when you walk by. When you aren't being shoved around by busy passers-by, you can spot your home here - it's number 333, the small red door next to the fishmongerss.


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Cancer Resources – Elkhart, Indiana – Direct Financial Aid
Although Cancer Resources is known for providing financial aid to Elkhart County cancer patients, our programs offer much more. When someone is diagnosed with cancer, they are most often emotionally overwhelmed. So, Cancer Resources’ caring staff walks every cancer patient through a comprehensive planning process.
Our theory is that when you enter a new phase of life, you’re always better if you have a plan. The problem with cancer, the medical industry, and the insurance industry is that most people don’t know where to start asking the right questions. Cancer Resources’ staff helps the cancer patient recognize where their strengths and deficits are, and helps them better understand how they can use Cancer Resources’ financial aid. In addition, Cancer Resources’ clients are overwhelmingly middle income people who’ve never asked a nonprofit organization for help. In 2016.
In 2016, Cancer Resources identified 22% of our clients who could potentially benefit from services offered by other organizations. UC’s referrals benefited our cancer patients by an estimated additional $151,000. In addition, our caring staff works to stay in contact with our clients to provide vital emotional support to them and their families as they face the stress and fear of cancer treatment. In fact in 2016, United Cancer’s staff made over 2,000 client contacts, averaging more than eight contacts per day.
In 2016, Cancer Resources dedicated over $252,000 to our programs which include both staff time dedicated to planning and emotional support, as well as over $125,000 in direct financial aid.
New for 2017, Cancer Resources is offering Wellness Programs including, Yoga and Reiki to help with exercise and relaxation, Nutrition Classes to help with healthy eating during cancer treatment, and Art Classes to help express and deal with the emotions of cancer treatment
Also new in 2017, UC has connected with Express Employment Professionals to provide employment counseling as well as help in finding physically appropriate work environments for recuperating patients. Contact us for more information about our direct financial aid.

domain: medical


text:
Foxconn's iPhone plant 'paralysed' as thousands strike
SAN FRANCISCO (REUTERS) - Thousands of workers went on strike on Friday at a Foxconn plant in China that makes Apple Inc's iPhone 5, paralysing production of the smartphone, rights advocate China Labour Watch reported.
The reported strike comes at a crucial time for the United States corporation, weeks after kicking off its largest-ever global rollout of the smartphone. Apple is already struggling with supply constraints, analysts say.
Citing workers, the labour group said 3,000 to 4,000 workers began their strike at Foxconn's Zhengzhou complex in the afternoon, incensed by over-exacting quality controls as well as demands they work through the week-long "Golden Week" holidays, which began on Monday.
The strike could not be immediately confirmed. Apple declined to comment and Foxconn was not immediately available for comment.

domain: news


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When an individual is seriously injured, he or she likely expects doctors to take the necessary steps in order to treat the injury. Unfortunately, some Ohio residents could be at risk of suffering further injury due to medical negligence on the part of doctors or other hospital staff. It was recently reported that a man in another state became paralyzed after doctors failed to treat a spinal fracture in time.
Reports stated that the man had been injured while working as a logger when part of a tree collided with his back. The incident resulted in his suffering a spinal injury that caused him severe pain. Because of the pain, the man was unable to position himself for a full set of spinal X-rays. Rather than order a complete CT scan for a more accurate view of the injuries, doctors apparently used the incomplete X-rays.
As a result of just using the X-rays, a fracture in the man’s spine was missed. He was hospitalized for three days, spent two more days at home and was later taken to another hospital. By the time the fracture was detected, the man had become paralyzed from the chest down. He filed a legal claim against the doctors associated with his care, and his case was successful. He and his wife were awarded compensation of more than $2 million.
Medical negligence can be frightening to experience, and as this case shows, serious and lasting injuries could result. If Ohio residents believe that they were negatively affected by the actions of medical professionals, they may wish to consider taking legal action of their own. Information on filing civil claims for medical malpractice may help interested parties determine if they would like to move in such a direction.
Source: therepublic.com, “Pennsylvania jury awards paralyzed logger’s family $2.2 million in medical malpractice lawsuit“, Oct. 26, 2015

domain: health


text:
Our Red Brahmans herd had some Gyr & Indu influence as well as some very nicely bred Beefy American Brahman style cattle.
375 T Johnson Road
Carthage, NC 28327
I'm a farrier by trade. I have had a team of Red Devon oxen and my daughter has Brahmans so I've become interested in them as well.
We have been raising commercial brahman and brahman influenced cattle for over 30 years. We love and are passionate about this great breed, its history, and its preservation!
We are excited about our Future Mommas for Rock Hill Brahmans. We think they are a tremendous set of Heifers with some top notch, well known Polled Genetics to back them. Right here in Central NC!
Own Daughters of +Mr V8 279/7
Miss HMC Polled 114/1 (also a daughter of the famous SRS Polled Katie)
Miss HMC Polled 98/1 (#JADL RE TE and #SMG Monumental on her Dam's side)
Own Daughter of Moreno Mr Polled Kyros 571:
Miss Ward Polled Paisley 29 (also Great Grand Daughter of V8 287/5 "Superstroke")
Our Current herd sire "NC Polled Satchel 259", is a polled Grandson of +LMC Polled Charley- who has just made Register of Renown in 2020
We also have some nice Dark Pigmented horned cattle we are seeing some awesome results out of when crossed with our polled bull.
Last January we added some registered Herford cows and have some Golden Certifiable calves hitting the ground.
Come check us out. We welcome visitors by appointment.
Pine Knot Cattle Co is located in central North Carolina and is owned and operated by Jeremy and Hayley Sessoms along with our two daughters Annie Blake and Oakley. We raise registered Brahman and Brangus cattle.
253 Odoms Chapel Road
Bakersville, NC 28705
Small Western NC farm with less and 20 cows. Home of Sire Otis Redding.


What is Combined Cycle Technology?
It is technology used in power plants to increase production. A power plant can generate 50% more electricity with combined technology than with a single-cycle power system. It uses waste steam that’s usually lost to generate additional electricity.
What Equipment is Involved in Combined Cycle Technology?
The typical equipment used in combined cycle technology includes:
- Gas turbines
- Waste heat recovery units for steam generation
- Steam turbines
- Other auxiliary equipment
How Does Combined Cycle Technology Work?
Combined cycle technology
is a dual phase system in which two combustion turbine generators operate in conjunction with two heat-recovery steam generators.
What are the Two Phases?
- First cycle: Two gas turbine generators burn natural or diesel gas to produce electricity.
- Second cycle: Hot exhaust that’s usually lost during the process is captured. The heat-recovery steam generators boil water to produce steam, which then spins another turbine generator, producing more electricity.
How Do Gas Turbine Generators Work?
Air is compressed and mixed with fuel heated to high temperatures, which makes the gas turbines blades spin. This spinning energy is converted into electricity.
How Do Heat-Recovery Steam Generators (HRSG) Work?
The exhaust heat from the gas turbine is captured by the HRSGs, which creates steam for the steam turbine. Additional energy is created when the steam turbine transfers its energy to the generator drive shaft.


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When Maine experienced its first case of COVID-19, the community action group Presente! Maine started sharing information about social distancing, staying home from work and hand washing with the Latino community it serves. But most said staying home wasn’t an option.
“The answer I was getting back from people was, ‘Thanks for the info but if I don’t work then I won’t eat and I won’t be able to provide for my family,’” Crystal Cron, president of Presente! Maine, told TODAY Parents. “Stay home and safe — that was just not even a possibility.”
Cron realized that her community needed food urgently, so she started a food brigade. At first they delivered food to 200 families. Then 300, then 400, then 800. On May 2, they delivered food to 1,200 families.
“It was very important for us to bring food directly to people. Not only are we feeding all these community members, but also we’re bringing it straight to their door,” she said. “You tell us you need food and you get some food.”
Cron expects the demand will increase as the pandemic continues. To continue, Presente! Maine started raising additional money on GoFundMe, where some people noted they were donating their pandemic stimulus checks to help. This is one of the many places that people have donated their stimulus money to help their neighbors affected by the coronavirus crisis. Such donations are being tracked and encouraged thanks to a campaign called Pledge My Stimulus launched by two moms in Maine. The movement encourages people to donate part or all of their stimulus checks to a nonprofit, local business or artist in order to help bolster their local economies.
Download the TODAY app for the latest coverage on the coronavirus outbreak.
“I was feeling a lot of gratitude,” Pledge My Stimulus co-founder Wendy Blackwell-Moore of Portland, Maine, told TODAY Parents. “My wife and I were still both employed. We have a roof over our heads, we have health care and I was thinking about so many folks losing their jobs. Quite frankly, there are so many people out there struggling to meet basic needs.”
At the same time, the Rev. Tamara Torres McGovern, who also lives in Portland, was mulling similar thoughts. The pandemic news sounded so dire that she felt “profoundly helpless.”
“As a leader in a church community, what we do is mobilize people to become helpers. There was this intersection between recognizing the need and what could be done,” Torres McGovern told TODAY Parents. “It was a golden opportunity for those of us who had not lost income to do something.”
Blackwell-Moore and Torres McGovern didn't realize they were stumbling upon the same idea: Maybe people with less of a financial need could donate their stimulus checks to the not-so-fortunate? Just hours apart, both women separately reached out to Maine State Rep. Victoria Morales to share the idea.
“Wendy and Tamara are two of my most amazing friends who inspire me,” Morales told TODAY Parents. “When the two of the have this amazing idea and they come to you on the same day, it is serendipity.”
Morales connected the two moms, and the campaign #PledgeMyStimulus was born. Since the campaign's website launched on April 6, more than 117 people have pledged some (or all) of their stimulus checks to almost 100 organizations in 20 states. So far, more than $88,000 has been pledged to nonprofit organizations, artists, local businesses, crafts and tradespeople.
“We are coming together in a way that is really inspiring,” Morales said. “Pledge My Stimulus gets to how we can be a community — and be with people — and still follow the CDC guidelines.”
The moms feel thrilled by the impact their campaign has had so far. But an unexpected benefit surfaced: Their children are learning how to navigate a scary situation while still thinking of others.
“As a mother of a 3-year-old watching her absorb the message of why we are staying away from people, it looks like we are afraid of other people,” Torres McGovern said. “(Now) what we ... are witnessing is hope and how we take care of one another.”
Blackwell-Moore, who has older children, CeCe, 14, and Jude, 10, agrees.
“What a beautiful distraction it is to be able to focus on this every day, instead of focusing on the statistics and the curves and the deaths,” she said. “One of the beautiful conversations we had when we committed to donating our stimulus checks was: Here is your home school work. Here is the money. Where are spending it?”
Jude and CeCe researched organizations and decided to donate some to local immigrant organizations because many of their classmates are immigrants facing greater insecurity right now.
“It is such a great opportunity (for our children) to learn how to give back and think about others,” Morales said.
The women hope this campaign will inspire people to continue to be thoughtful as they navigate ongoing economic challenges presented by the pandemic.
“It is going to be a long time before things feel normal, so how can we practice this economy of caring?” Torres McGovern said. “I am an optimist. My hope is that this gives us an opportunity to practice caring more deeply.”
CLARIFICATION (May 7, 2020, 10:14 a.m. EST): This story has been updated to clarify that Pledge My Stimulus is independent from Presente! Maine's GoFundMe.


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The Tale of the Fairy Tale
Doctor Unfortunate. No one knows who he really is or where he comes from. Not even his publisher knows who he is. All anyone knows of him is that he travels around and collects what he considers dark, scary and creepy stories to share with others.
I see a copy of Snow White and the Seven Dwarves lying out on a table in the school library. No doubt someone who loved fairy tales was reading it for fun. That’s the thing about fairy tales. When you’re younger, you enjoy reading them because they seem so fun and seem like an easy escape from the real world. When you’re older, they seem childish and like they have no point to them.


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Date(s) - 11/02/2017 - 14/05/2017
The Ed Mirvish Theatre
The breathtakingly romantic thriller THE BODYGUARD comes to Toronto and features the classics including So Emotional, One Moment in Time, Saving All My Love, Run to You, I Have Nothing, Greatest Love Of All, I Wanna Dance With Somebody and one of the greatest hit songs of all time – I Will Always Love You. The Canadian premiere engagement stars the acclaimed UK cast direct from its London West End run.
Tickets available at mirvish.com.

domain: entertainment


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A return to guitars and darker sound on Hagar’s last album suggested a much different band on the horizon.
The veteran rockers will be on the road from early July through late September.
The Tool vocalist appears on the former Van Halen singer’s show on AXS TV.
“I got diners in the back, drive-in is attached.”
Tune in this summer for the bizarre pairing of guests on Hagar’s TV show.
Nothing materialized from the conversation Anthony had with the Van Halen team.
The former Van Halen singer adds fuel to the rumors that the bassist could rejoin Van Halen.
At least for one day, Tool have themselves a new frontman.
The 25-song set featured appearances from Paul Stanley, Perry Farrell, Sammy Hagar, Stewart Copeland, Steve Jones, and Jason Bonham.
A nostalgic trip through decades of ingenuity, all without a DeLorean.
Former Van Halen singer comes to defense of the band’s one-time bassist, Michael Anthony.
Just wait ’til you see the tour name.


Springtown Police Department
Find your accident and request a copy of your accident report from Springtown Police Department.
January 1st 2019
SPRINGTOWN POLICE DEPARTMENT
Obtain a Copy of your Car Accident Report
How to obtain a Car Accident Report in Springtown, TX
|In Person:||102 E. 2nd Street, Springtown, TX, 76082|
|By Mail:||102 E. 2nd Street, Springtown, TX, 76082|


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The Edo State Government has taken over the construction of the Lawani Crescent Federal Constituency Road project in Akoko-Edo Local Government Area of the state.
Following a viral video online, which exposed the poorly executed road project, I, represented by my deputy, Right Honourable Comrade Philip Shaibu, inspected the project and affirmed that it is substandard; falling short of the standard that we have maintained in execution of road projects across Edo state.
We thank the whistleblower for raising the alarm over this bad job and call citizens to take charge of all government properties in their area.
The contractor handling the Lawani Crescent project must be arrested for the bad job done and handed over to I.C.P.C and the E.F.C.C, to enable us ascertain exactly what happened.
The project stands revoked and the state government is taking over the reconstruction of the road immediately.
N360m is what is allocated to Akoko-Edo constituency projects by the National Assembly this year. We would investigate all that to ensure we have value for money.
@views exclusive rights: Godwin Obaseki, Governor, Edo State, July 6, 2021.
- Video Showing David Umahi Ordered Soldiers To Flog Civil Servants Doctored – APC
- Video Suggests David Umahi Orders Soldiers To Flog Civil Servants Over Lateness To Work In Ebonyi
- Lagos Increases All Workers Salaries To Fight Nigeria Inflation, Rising Cost Of Living
- IGP Builds Police Stations With Air Conditioners
- IPOB Warns Atiku
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Our service line provides the most advanced surgical options available from laparoscopic surgery, single incision laparoscopic surgeries, incisionless endoscopic procedures, to robotic surgery, easily adapting to the developing technologies of the medical world.
Professor of Gastroenterology
Anadolu Medical Center is one of the most modern, comprehensive and respected hospitals in Turkey. Anadolu is affiliated with John Hopkins Hospital.
The Gastroenterology Institute at Rambam Health Care Campus diagnoses, treats, and monitors patients with diseases of the gastrointestinal tract and liver as well as those who suffer from inflammatory bowel diseases.
Director, Institute of Gastroenterology
Medipol Mega Hospital Complex is a modern medical facility with four specialist hospitals and an extensive selection of high caliber medical devices available for use. The hospital provides treatments in a wide variety of medical fields in its 470 bed facility.
Pediatric Gastroenterology, Hepatology and Nutrition Specialist
Gastroenterology and Hepatology Specialist
In the Division of Gastroenterology, local, national, and international patients receive world-class diagnostic evaluations, treatment, consultation, and follow-up care in the full complement of internal medical disciplines.
Gastroenterology Depatment Head
Gastrointestinal Cancer Department Unit Director
The second largest hospital in Israel, Rabin Medical Center is a tertiary care hospital that can handle the most complicated cases in all medical fields.
Inflammatory Bowel Disease
A private hospital located on the shorelines of Herzliya, just off Tel Aviv, offering healthcare services to both local and foreign patients. The hospital is affiliated with over 500 Israeli physicians, many of whom hold high level positions at public hospitals.
Liv Hospital is the only institution in Turkey co-authorized Center of Excellence Accreditation in colorectal surgery, robotic surgery and bariatric surgery by the Surgical Review Corporation (SRC) and provides advanced technology and treatments to its international patients with its 159 bed capacit
Head of Gastroenterology, Gastroenterology and Internal Diseases Specialist
Şişli Florence Nightingale Hospital is one of the few leading health care institutions, which combines state-of-the-art medical technologies with a large and experienced team and the excellent service approach adopted in all of its operations.
Kadıköy Florence Nightingale Hospital provides services in all specialties with its inpatient and outpatient, diagnosis, treatment and emergency service facilities and fully-equipped polyclinics.
- Gastrointestinal endoscopic- Inflammatory Bowel Disease
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Dubai: There is good news for frequent Dubai travellers or business professionals as the United Arab Emirates made a big change in their visa policy.
Regarding UAE visa, the country initiated a multiple-entry visa scheme for people from all countries valid for the next five years, reason being to change the setting from a Gulf status to that of a tourist fulcrum.
The government of Dubai Media Office tweeted, “#UAE Cabinet chaired by @HHShkMohd, approves new amendment for tourist visas in #UAE,” remarking to the UAE Ruler and Prime Minister of Dubai Mr. Sheikh Mohammed bin Rashid Al Maktoum.
Quoting the Dubai Media Office, ‘the new tourist visa will be applicable to all nationalities and could be applied for multiple-entries validating for the coming 5 years.
Sheikh Maktoum on Twitter mentioned that the UAE at present caters to 21 million tourists a year.
Majority of the travelers from countries like Africa, South America, European states from outside the European Union and former Soviet Union to even Arab states outside the Gulf had formerly needed visas.
Dubai is expected to host Expo 2020, the big ticket global trade fair in October.


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Providing the ultimate retreat from the demands that often come hand in hand with everyday life, the Waterfront Spa and Health Club is not only a great place to escape the stresses of life, but it also provides the perfect space to indulge in some much needed ‘me time.’
The boutique Waterfront Spa & Health Club in Dover, offers an intimate and inviting pamper experience. With facilities which include a fitness room, Jacuzzi, steam room, sauna, and seven beautifully appointed treatment rooms and relaxation area.
Enjoy a relaxing day with a friend, complete with fluffy robe, a choice of one 25 minutes pampering treatment, use of the Spa and gym facilities and lunch.
You can choose one from the following, each: Swedish Back Massage, Express Elegance Facial, Express Mani, Indian Head Massage, Heavenly Hydration Salt Brushing.
Please inform the spa of any skin allergies or medical conditions that may affect you taking part. A health check will be required which may result in the spa requesting a medical certificate.
The minimum age for this experience is 18 years old.
This experience is available Monday to Sunday subject to availability, throughout the year subject to opening times and availability.


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What is the old name of South India?
South India, also known as Peninsular India, has been known by several other names.
What are South Indian called?
Karnataka, Tamil Nadu, Andhra Pradesh, Kerala, Telangana and Puducherry are all different states and a union territory we have in the South and people belonging to these states are not all Madrasis.
What is the old name of Tamilnadu?
In 1969, Madras State was renamed Tamil Nadu, meaning “Tamil country”.
What was North India called in ancient times?
The name literally means ‘Land of the Jambu tress’. Though not all of India, Aryavarta was the name for the Northern region of India. A name that is still used, the Persians named India Hind or Hindustan. India is officially called Bharat or the Republic of India and is called so after the Ruler Bharata.
Which is the oldest civilization in South India?
Indus valley civilization is bronze age civilization which pre-dates, Keeladi, which is an Iron age civilization. Excavations are in budding stage in Vaigai valley civilization. INDUS inscriptions are widely discovered among Rock art paintings in South India.
Which were the ancient dynasties of southern India?
The Cher, Pandya, and Chola dynasty was the ancient dynasties of Southern India.
Why are East Indians called East?
After Portugal handed over Bombay in 1661 to the British East India Company, the company began recruiting Christians from other parts of the Konkan — Mangalore and Goa. … In order to differentiate the ethnic community of Bombay from the migrants, they began to call themselves the Original East Indians, after the company.
What is South India famous for?
Southern India has one of India’s most visited pilgrimage spots. The prominent one being the Balaji Temple at Tirupati in Andhra Pradesh. Also, the ancient Buddhist site of Nagarjunakonda, and the wonderful Islamic buildings of the Qutub Shahi and Asaf Jahi dynasties at Golconda Fort and Hyderabad reside here.
Why was South India developed?
The southern States of India present a unique model of development. They have become the country’s economic engine and the bastions of social progress. The region attracts multinational IT (services) and automobile (manufacturing) firms while remaining the spice capital (agriculture) of the world.
What is the old name of Kerala?
Entire Kerala was once known as Malabar in ancient times. After the arrival of British, Malabar became a geopolitical term to refer for North Kerala and they divide it further into North Malabar and South Malabar. Malayalam language is called Malabar in Spanish and Portuguese languages.
What is the old name of Chennai?
Chennai, originally known as Madras Patnam, was located in the province of Tondaimandalam, an area lying between Pennar river of Nellore and the Pennar river of Cuddalore. The capital of the province was Kancheepuram.
Who invented Tamil?
According to Hindu legend, Tamil or in personification form Tamil Thāi (Mother Tamil) was created by Lord Shiva.
What was another name for the ancient Indians?
Jambudvipa (Sanskrit: जम्बुद्वीप, romanized: Jambu-dvīpa, lit. ‘berry island’) was used in ancient scriptures as a name of India before Bhārata became the official name.
What was the ancient name of Orissa?
Orissa was known as Kalinga in ancient days. Another ancient name of this region was Utkal. It is widely known as the land of Lord Jagannath.
Is India mentioned in Bible?
India is mentioned in Esther 1:1 and 8:9 as the eastern boundary of the Persian Empire under Ahasuerus (c. fifth century B.C.) and in 1 Maccabees 6:37 in a reference to the Indian mahouts of Antiochus’s war elephants (second century B.C.). Otherwise there are no explicit references to India in the Old Testament.


New Louisiana Bill Will Finally Set A Minimum Age for Marriage
Yesterday a house committee advanced a bill that would set a minimum age for marriage in Louisiana. You heard that right, with proper parental consent, there is no such legal thing as "too young" to get married in the Bayou State. According to a report from the Bossier Press:
Minors need parental consent to get married, and if they are under 16 they need parental consent and the authorization from a juvenile court judge."
In Texas the minimum age is 16 with a judges consent, and in Arkansas it's 17 with parents permission. Louisiana is among the 16 states that don't legally define a minimum age, but that may soon change. A new bill introduced by Sen. Yvonne Colomb, D-Baton Rouge, would set that firmly at 16 and would require parents permission. Rep. Tanner Magee, R-Houma, and Rep. Mike Johnson, R-Pineville both argued that 16 was still too young and suggested that the age be raised to 18 years old.
The proposed law, with the agreed upon minimum age off 16, has made it past the first set of legal hurdles in the state senate and is well on its way to becoming a law. The new marriage rules now move to the house for final passage.


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Digestive health is something that is important for humans and animals. We talk with Blake Jackson, owner of Rogue Pet Science, about their wholistic approach to dog digestive health and their product origins among many other dog health topics. Check it out!
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– Alright, hey everybody. I’m Courtney here from DogBoy’s and we’re here with Blake from Rogue Pet Feed and today I wanted to have Blake tell us a little bit about this awesome product that they carry called Origins. So Blake, tell me a little bit about Origins and what’s in it.
– Awesome, so Origins is our supplement. It’s a wholefood topper and it does gut health as a system. The reason why we created Origins was we needed a product that could be easily implemented and was cost effective to help dogs. And so this is something that an owner can take and just add to their dog’s food. It helps them digest their dog’s food better and it gets them all their probiotic, pre bod, the digestive enzymes and the butyric acid that they need to really address the system as a whole. And with that whole systems approach, we’re actually able to impact the health of the animal.
– It’s an amazing product. I mean, just with my own dogs, they have really thrived on it but I just wanna know a little more about how you guys got into that business and why it’s so important for dogs.
– Sure, so the story behind how Rogue started and how Origins came about is really Nate and I, Nate is our scientist and founder and we actually met at the gym and so it’s your kinda classic bromance story. We started out lifting weights together and then we quickly realized we had both had actually adopted dogs at the time in Galveston. Everything we do, we take it way too far and Nate was actually training for Strongman at the time. I ended up training for body building in our own personal fitness endeavors and then with the dogs, we kinda took the same route. We started looking at nutrition for our dogs and how we could get more out of the dogs. We were doing everything back then like trying raw and olive oil, and we would see good improvements by making these changes to dog food ’cause clearly there was something there, there was something missing and so Nate went back to school. He went to grad school, A&M and he got further into the animal feed world. He started working with the largest production animal facilities globally, and he started consulting with them on feeding strategies and herd health strategies. And he quickly saw that they had a very systematic approach to health and it all started with the health of the animals starting in the gut. He started looking back at the dog food thing going well no wonder there’s a problem and he looked at how dog food was made and was like this is where there’s an opportunity. There’s really an opportunity to improve on this. Overtime, he started working with different working dogs to begin with ’cause our own heart was in performance and so with the combination of the probiotic, the pre bod, the digestive enzymes and butyric acid and then the highly digestible wild fish that he sourced. We started seeing some improvements that we knew were possible from the production animal world happening with dogs. And that’s when we knew that we were on the right track. Yeah, this allows owners to be able to implement raw level nutrition. The digestibility is truly equal to raw or feeze dried for pennies a day.
– That’s great. And the main protein in it is, you said it’s a type of fish?
– What type of fish is it?
– It’s a menhaden species of fish. It’s a wild caught fish that eats a wild diet. Super high in omegas. We do source that direct so we have full traceability on the ingredients. The place that manufactures us fish, this is the only thing they make. So they’re not processing other animals in that facility or making HEB salads around the corner or something like that. All they do is make this fish, and so we don’t have to worry about any weird contamination happening with it.
– The other thing that was interesting to me is that it’s not only a sustainable fish but it’s a type of fish that if you didn’t fish it in the wild then it would actually be considered like the weed of the fish world. Like it would over populate and there would be too many of them.
– Yeah, one female can lay up to like 400,000 eggs each year and so they actually pull out about 450,000 tons at this one facility and process it and they’re not even touching the schools, populations. So it’s very sustainable because the fish is small and it eats a phytoplankton diet, it’s super high in omegas. This is actually one of the species of fish they use for making human grade fish oil.
– Oh wow!
– That’s wonderful! Well I know I’ve used it with my own dogs. My older dog, Rebel has a lot of skin problems, he’s got some funky stuff on his head right now like a bacteria but this is really helping it go away faster and keeping it a bay so it’s been great for my dogs.
– Awesome. Yeah, regrowing hair and getting rid of allergies is something we’re getting to be known for so a lot of it stems just from increasing digestibility but that fish oil does a ton for inflammation and set things straight.
– Tell me a little bit about the famous breed line that’s come through, not necessarily who the famous dog is but I know you guys have been working with dogs who were having problems getting pregnant and that this made a big difference for those dogs.
– Yeah, so the gut’s really impacts all systems of the dog. It impacts all health of the dog when done correctly. And so because of the science that Nate had access to that show that you’re able to impact reproduction in production animals with this strategy, we knew it would happen with dogs and so we already had many reports of older dogs coming back into heat well passed where they should be 12, 14 year old dogs coming back into heat so we knew it was working and we got connected because of that with a very famous line of Collies. We won’t name the Collie but I’m sure you can guess. We worked with their vet directly and so the vet, the first thing we had to do is we had to make sure there wasn’t a genetic problem that was keeping the dog from being able to have a litter of puppies. And after we had that test and everything was good on the genetic side, we implemented the Origins and it was simply just add this to the dog’s diet. There was no other changes made. The vet that manages that dog did all the blood draws and sent it off to our lab in Houston to have it checked out and quickly saw that we were actually making improvements that they don’t see. And so she was very excited by that and offered to help us more. So we had a completely unsolicited opportunity to have a vet pick out some dogs, preexisting health conditions, altered, unaltered, and actually do a real world study with Origins so we were able to complete that. The data’s back in. They looked at four different biomarkers. We were able to help with all of them just by adding this to a dog’s diet and in 30 days we had improvements in all areas of anti-inflammation, anti-cancer, reproduction, and metabolic markers.
– That’s just, that’s amazing results. And the thing that I think a lot of people don’t know. We have a lot of people who come in whose dogs have a lot of health problems, and I used to be able to say, oh well you need to change the dog’s diet and the health problems will go away but what we’re finding is that changing the diet isn’t fixing a lot of these problems and you’re the first person that, you and Nate are the first people that I’ve ever heard of who really know why that’s happening. Why is it that good quality, high end, expensive dog foods aren’t doing the trick of getting rid of dogs with allergies and skin conditions and things like that?
– Yeah, this is all thanks to Nate really understanding the processes and what’s happening to the molecules in the food as it goes through the process. So we have a lot of really well-intentioned pet food companies that realize there’s a deficit with dog food. Their solution right now is to try to pick better ingredients and put it into a system that basically is gonna end up making it not available. I don’t think they’re doing it unintentionally or anything with any malice or anything, I think it’s just this is the system we use and they’re just turning to the same system trying to fix the problem the way they can. What’s happening is when you take ingredients, even if you remove all the grain and you put it through extrusion, you’re using pressure and heat to cook it and it ends up getting cooked three times as it go through the process of extrusion. Well, the interesting thing about that is that every time you cook it and pressurize it you’re creating more bonds. And so now you’ve removed all the grain, but now you’ve created a heavily bonded ingredient list that looks just like grain to a dog.
– Right, so even if it’s grain-free it’s still, it’s so difficult for the body to break it down that it acts like a grain.
– Yeah, because dogs only make the enzymes to break down fat and protein. They don’t actually make the enzymes necessary to break down heavily bonded stuff like grains and so now you’ve removed the grain and then you’ve created something that looks just like grain. So Origins allows you to get more of that product out of the dog’s diet and also provides all of the enzymes that will allow them to break down grains so that they can deal with that better. And so you end up having less of an immune response and that’s one of the ways that we’re able to help with allergies. And that’s not just food allergies, that’s all allergies.
– Seasonal allergies too.
– Yeah, seasonal and everything because the higher the amount of immune response that’s going on with the dog, the sooner all the allergies show up whether they’re environmental or food related.
– Right. Oh, that’s great. So basically everything that your dog should be getting out of good quality food but isn’t.
– Wow, well thank you so much for joining us today, I really appreciate having you on.
– Yeah, thanks for having us out. We’re glad to have y’all representing the product.
– Alright, well if you want more info about Origins, come see us at DogBoy’s. We’d love to tell you more about it.


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RIGHT NOW, SAVE ON OUR CONCUSSION COURSES
– Functional neurology management of Concussion
– Concussion Bootcamp
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Pain Reset – Chicago
October 22, 2020 @ 9:00 AM - October 25, 2020 @ 9:00 AM EDT$2,495.00 – $2,995.00
To learn more about the Pain Reset Program, please visit the main program page by clicking here. TRAVEL UPDATE Flying into O’Hare International Airport Passengers traveling to the City of Chicago from Alabama, Arkansas, California, Florida, Hawaii, Georgia, Idaho, Iowa, Kansas, Louisiana, Mississippi, Missouri, Nebraska, Nevada, North Carolina, North Dakota, Oklahoma, Puerto Rico, South Carolina, South Dakota, Tennessee, and Texas are subject to a mandatory 14-day quarantine in a home or hotel room. For more information, visit the Illinois Department of Public Health, opens in a new window. We recommend traveling into General Mitchell International Airport, Milwaukee, WI 53207, and renting a car to drive to the venue. Schedule Thursday 9:00am – 6:00pm Friday 9:00am – 6:00pm Saturday 9:00am – 6:00pm Sunday 9:00am – 3:00pm