By Quan Li
The chemistry, physics, and functions of liquid crystals beyond LCDs
Liquid Crystals (LCs) mix order and mobility on a molecular and supramolecular point. yet whereas those amazing states of topic are most ordinarily linked to visible reveal applied sciences, they've got very important functions for numerous different fields to boot. Liquid Crystals past monitors: Chemistry, Physics, and Applications considers those, bringing jointly state-of-the-art learn from the most promising components of LC science.
Featuring contributions from revered researchers from all over the world, this edited quantity emphasizes the chemistry, physics, and functions of LCs in parts resembling photovoltaics, light-emitting diodes, filed-effect transistors, lasers, molecular cars, nanophotonics and biosensors. particular chapters examine magnetic LCs, lyotropic chromonic LCs, LC-based chemical sensors, LCs in metamaterials, and masses more.
Introducing readers to the basics of LC technology by using illustrative examples, Liquid Crystals past Displays covers not just the latest learn within the myriad components within which LCs are being applied, but additionally seems to be forward, addressing strength destiny advancements. Designed for physicists, chemists, engineers, and biologists operating in academia or undefined, in addition to graduate scholars focusing on LC expertise, this is often the 1st booklet to contemplate LC purposes throughout a variety of fields.
Chapter 1 Liquid Crystal Lasers (pages 1–27): Hideo Takezoe
Chapter 2 Self?Organized Semiconducting Discotic Liquid Crystals for Optoelectronic functions (pages 29–82): Chenming Xue and Quan Li
Chapter three Magnetic Liquid Crystals (pages 83–110): Rui Tamura, Yoshiaki Uchida and Katsuaki Suzuki
Chapter four Ferroelectric Liquid Crystals for Nonlinear Optical purposes (pages 111–156): Yongqiang Zhang and Jesus Etxebarria
Chapter five Photo?Stimulated section changes in Liquid Crystals and Their Non?Display functions (pages 157–211): C. V. Yelamaggad, S. Krishna Prasad and Quan Li
Chapter 6 Light?Driven Chiral Molecular Switches or automobiles in Liquid Crystal Media (pages 213–249): Yan Wang and Quan Li
Chapter 7 Liquid Crystal?Functionalized Nano? and Microfibers Produced by means of Electrospinning (pages 251–284): Jan P. F. Lagerwall
Chapter eight practical Liquid Crystalline Block Copolymers: Order Meets Self?Assembled Nanostructures (pages 285–301): Xia Tong and Yue Zhao
Chapter nine Semiconducting purposes of Polymerizable Liquid Crystals (pages 303–339): Mary O’Neill and Stephen M. Kelly
Chapter 10 Liquid Crystals of Carbon Nanotubes and Carbon Nanotubes in Liquid Crystals (pages 341–378): Giusy Scalia
Chapter eleven Liquid Crystals in Metamaterials (pages 379–402): Augustine M. Urbas and Dean P. Brown
Chapter 12 Ferroelectric Colloids in Liquid Crystals (pages 403–426): Yuriy Reznikov
Chapter thirteen truth or Fiction: Cybotactic teams within the Nematic part of Bent middle Mesogens (pages 427–448): Bharat R. Acharya and Satyendra Kumar
Chapter 14 Lyotropic Chromonic Liquid Crystals: rising purposes (pages 449–484): Heung?Shik Park and Oleg D. Lavrentovich
Chapter 15 Liquid Crystal?Based Chemical Sensors (pages 485–504): Jacob T. Hunter and Nicholas L. Abbott
Chapter sixteen Polymer Stabilized Cholesteric Liquid Crystal for Switchable home windows (pages 505–523): Deng?Ke Yang
Chapter 17 Liquid Crystals for Nanophotonics (pages 525–567): Timothy D. Wilkinson and R. Rajesekharan
Read Online or Download Liquid Crystals Beyond Displays: Chemistry, Physics, and Applications PDF
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Extra info for Liquid Crystals Beyond Displays: Chemistry, Physics, and Applications
S. M. Morris, A. D. Ford, and H. J. Coles. Removing the discontinuous shifts in emission wavelength of a chiral nematic liquid crystal laser. J. Appl. Phys. 2009, 106, 0231121–023112-4. 48. Y. Huang, Y. Zhou, C. -T. Wu. Tuning the photonic band gap in cholesteric liquid crystals by temperature-dependent dopant solubility. Opt. Express 2006, 14, 1236–1242. 49. M. Ozaki, M. Kasano, T. Kitasho, D. Ganzke, W. Hasse, and K. Yoshino. Electro-tunable liquid-crystal lasers. Adv. Mater. 2003, 15, 974–977.
This is because TOF mobility is measured over relatively thick samples (usually tens of microns) on a millisecond or microsecond timescale and therefore is more susceptible to defects and grain boundaries. Nevertheless, TOF experiments lead to higher mobility values over OFET and SCLC techniques, which yield one order of magnitude lower. This discrepancy is because the carrier injection efﬁciency is lower between the metal electrodes and the organic material in both SCLC and OFET devices . Furthermore, charge transport behavior in DLC materials with multiscale conformational dynamics were computationally investigated .
M. Humar, M. Ravnik, S. Pajk, and I. Musevic. Electrically tunable liquid crystal optical microresonators. Nat. Photon. 2009, 3, 595–600. 74. V. Snadoghdar, F. Treussart, J. Hare, V. -M. Raimond, and S. Haroche. Very low threshold whispering-gallery-mode microsphere laser. Phys. Rev. A. 1996, 54, R1777–R1780. 75. Q. J. Wang, C. Yan, N. Yu, J. Unterhinninghofen, J. Wiersig, C. Pﬂugl, L. Diehl, T. Edamura, M. Yamanishi, H. Kan, and F. Capasso. Whispering-gallery mode resonators for highly unidirectional laser action.


The Georgia Municipal Association, Inc. (GMA) is soliciting proposals from firms interested in providing Construction Management At-Risk services for the construction of a project known as the Georgia Municipal Association New Headquarters and Renovation Project, located in Atlanta, Georgia, consisting of a new building, a parking deck, and a renovation of an existing building. This Request for Proposal (RFP) seeks to
identify potential providers of the above-mentioned services. Some firms that respond to this RFP, who are determined by the GMA to be sufficiently qualified, may be deemed eligible and may be invited to interview and offer a fee proposal in a sealed envelope for these services.
The RFP document is available to download here
. Interested firms are advised to check this site periodically for additional project related information and updates.


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NPR is marking Philip Glass’s upcoming 80th birthday by asking a few of his collaborators and colleagues to write about him and his music. Paul Simon contributed lyrics to “Changing Opinion,” a song from Glass’ 1986 album Songs from Liquid Days. Here is an excerpt from Paul’s essay at NPR:
As a musician, I am fascinated by Glass’s use of repetition and symmetry. The way he subtly breaks patterns by changing time signatures, or playing different time signatures (a three against two), to interrupt the repetition before it numbs the mind. It allows the listeners to swim in an almost trance-like state until a new musical motif is introduced.
… His use of what he calls an “end piece” — a short coda which does not recapitulate the melodic lines of the larger preceding piece — is an idea that he used beautifully to conclude my song “The Late Great Johnny Ace.” That end piece concept has found its way into my arrangements for live shows, as the band plays a related but original addition to a song, allowing me to shape endings to sequences of songs, or to set the environment for the next tune.
Read more from ‘Swimming In A Trance-Like State’: Paul Simon On Philip Glass at NPR.


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The costs of acquiring new users for mobile apps reached an all-time high in December as well-heeled companies scrambled for mobile market share over the holidays, according to a report by mobile marketing firm Fiksu.
The latest group trying this sort of thing out:
Boost Mobile customers can reduce their phone bills by $5 per month by displaying targeted ads, offers and content from brands and media companies including Starbucks, Levi’s and Lyft at various times when they unlock their handset.
Article on shady lead gen and dependent industries:
To understand how lead gens game Google’s algorithm, you need to know a bit about how a company’s address winds up in Google’s results, and how those results can be fraudulently altered. Few know the particulars better than Bryan Seely, who worked for a lead-gen outfit in the automotive glass repair field, based in Southern California, from 2008 to 2010. During that time, he produced about 3,000 fake auto glass repair listings across the country. Eventually appalled by his handiwork, he became what he calls a reformed hacker and explained his techniques in a self-published book, “Cyber Fraud: The Web of Lies.”
Gets really deep into Neustar:
By implementing these government data requests with precision and accuracy, trusted third-parties — like Neustar, Subsentio, and Yaana — can turn reasonable profits for their services.
Sources say Twitter made the move in an attempt to get some of its VIP users to stay engaged with the service.
CPMs for all Instagram ads — that includes video and carousel ads, too — were $5.21 in September, peaked in November at $7.20, and then fell back to $5.94 in December.
DataXu’s valuation is not being disclosed in this round, but CB Insights, which compiles finance data on startups and other tech companies, tells us that it has marked DataXu as part of the “unicorn club”– with a valuation over $1 billion — since 2015. DataXu tells TechCrunch that its 2014 revenues were $167.5 million, up from $100 million the year before. (These are the latest figures the company discloses, a spokesperson tells me.)
Amazon has discussed working with phone brands at a “factory level” to integrate its services with devices in a deeper way than simply preloading apps as it currently does with some companies, says one person familiar with Amazon’s thinking. This would help Amazon gain a measure of influence over Android smartphone software, which is owned by a competitor, Google.
The Ghostery community has grown to more than 40 million and has garnered support from transparency and privacy advocate Edward Snowden. However, roughly half of those people have opted into the GhostRank extension, which is how Ghostery makes its money. When you opt in, Ghostery anonymizes your Internet footprint and sells it to marketers. Of course, there has been some hesitation and mild pushback. Ghostery sells data to marketers — even if it is anonymous.

domain: news


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Handmade Posh Dog Jumper - 039 - The Chain Link Pattern size S
The 'preppy' look
1 in stock.
Hand knitted dog jumper from the 'Chain Link' collection
Made locally in the Lake District
To fit the tinies
Total length: 8 1/2" + the roll neck Around chest: 11" Around neck: 9"
The 'Chain Link' pattern jumper is a whole body jumper with a roll neck which will keep the chest and shoulders snuggly and warm. These are usually made in a 3 colour pattern stripe with a raised 'chain link' to the pattern on the colour change. The wide, snug under chest piece is plain and has a texture to it, and button holes that do work if you need them too. The leg hole is wide and soft - so you don't need to keep undoing the buttons if you don't want to
Colours - Forest Green / Navy / Mustard Buttons - 6 mis-matched yellows
Measurements are very approximate - there is quite a lot of 'give' in these.
Made in Robin 100% acrylic yarn. Machine washable on a cool wash - do not iron.
All my hand knitted dog wear has a dog shape button detail.

domain: design


SHOALS — The Martin County Commissioners voted Tuesday night, to sell 2 of their 4 acres remaining in the WestGate Tech Park to Chris Baugh of Loogootee for $15,000. Baugh will be building a not-yet-announced business on the acreage.
It was discussed at the last redevelopment commission meeting that Baugh may be requesting tax abatement in the future.
Economic Development Director Tim Kinder noted that the 2 acres have not been on the tax rolls in the county for 70-plus years.
Commissioner Dan Gregory said there are covenants and restrictions on buildings built in WestGate that Baugh has agreed to abide by. Gregory wished Baugh good luck with his endeavor.
Rhonda Rumble with SIDC and Lost River Township Trustee Millie Brown spoke to the commissioners about the Lost River Fire Station grant project. Rumble presented the commissioners with a subrecipient agreement to sign that is an agreement between the county and the fire station to work together.
She also reported that the fire department is working with the engineer to get the final design for the building together and bids will be let next spring. The completion date set by the grant is March of 2014 but Rumble said they are on track to be ahead of schedule.
Julia Albright gave the commissioners a new ordinance regarding fee increases for the health department. The fee for annual food permits was increased from $25 to $50 for permanent establishments. The fees for exempt food establishments and temporary permits per site/event remained the same.
Septic permits were increased from $30 to $50, dye testing stayed at $30, and well water testing went from being free to $10. Birth and death certificates went up from $7 to $8 to $10 depending on the type and to register or amend a birth certificate went up from $24 to $25. The cost for a genealogy search with up to five pages of information went from being no charge to $5. Each additional page is $1.


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Publisher. A. Martinez Lorca. Prostate cancer is the most frequent malignant neoplasia and holds third place in mortality among males in many countries.
Author Archives: Administrador
Original Articles. J.E. Sedano-Basilio, et al. There are no reliable epidemiologic registers of cancer in Mexico. Genitourinary cancer represents 20% of all cancers.
Originlal Articles. C.I. Villeda-Sandoval, et al. Prostate cancer is the first cause of mortality related to malignancy in Mexican men. Common clinical practice has to be evaluated in order to gain a picture of reality apart from the guidelines.
Original Articles. J.E. Ceballos-López, et al. Percutaneous nephrolithotomy (PNL) is the standard procedure for kidney stones larger than 2 cm, thus displacing open surgery. The incidence of complications varies from 12.5 to 52.5%, the main ones being fever and bleeding. Aim: To identify the factors associated with complications
Original Articles. J.A. Herrera-Muñoz, et al. Transurethral resection of the prostate (TURP) is a standard procedure for obstructive prostatic growth.
Original Articles. J.E. Sedano-Basilio, et al. Extracorporeal lithotripsy effectiveness is limited in lower calyx stones. Ureteroscopy currently enables complete access to the collecting system.
Original Articles. D.A. Preciado-Estrella, et al. During testicular cancer progression, retroperitoneal activity can condition urinary compression, especially in advanced stages. When this presents, it requires urinary diversion.
Clinical Cases. I. Camacho-Guerrero, et al. Rhabdomyosarcoma is the most common soft tissue sarcoma in childhood, more frequently affecting males, with an incidence of 8 children per one million.
Clinical Case. D.A. Preciado-Estrella, et al. The incidence of bulky kidney tumors has decreased thanks to the widespread use of radiographic studies that enable early diagnosis and treatment. However, there are still cases of giant tumors that are true surgical challenges.
Clinical Cases. A.F. Sánchez-Bermeo, et al. Cancer of the penis presents in individuals between 50 and 70 years of age, representing only 2-5% of the urogenital tumors in men.
Clinical Cases. J.S. Izquierdo-Luna, et al. Leydig cell hyperplasia is a rare benign condition characterized by small, multifocal testicular nodules that are frequently bilateral. Its form can be primary or secondary.
Clinical Cases. I. Jiménez-Vázquez, et al. Mesoblastic nephroma is a tumor that classically appears in childhood, and rarely in the adult. We present herein a case in an adult woman.
Clinical Cases. U. Sánchez-Aquino, et al. Schwannomas are well-encapsulated tumors arising from the peripheral nerve sheaths and they appear in young or middle-aged adults.
Publisher. E.I. Bravo-Castro, et al. Squamous cell carcinoma of the penis is a rare malignant tumor.
Original Articles. M. Pelayo-Nieto, et al. The procedure of transrectal biopsy of the prostate (TR BP) is not exempt from complications.
Original Articles. I. Jiménez-Vázquez, et al. To analyze the clinical, imaging, and surgical factors that are associated with failed double- J stent placement in patients with obstructive uropathy due to cancer.
Original Articles. A.A. Cayetano-Alcaraz, et al. In 2008 in Mexico, prostate cancer (CaP) held first place as cause of death due to malignant tumors in men.
Original Articles. I.A. Ramírez-Galindo, et al. Retrograde intrarenal surgery (RIRS) for the treatment of kidney stones has increased in the last few years. The S.T.O.N.E. score has been described as a reproducible tomographic evaluation tool enabling the complexity of urinary calculi to be estimated, thus establishing predictive factors for therapeutic success.
Original Articles. S.A. Gil-Villa, et al. To present the first year experience of the robotic surgery project at the Hospital Central Militar that consisted of 55 consecutive cases of robotic-assisted laparoscopic radical prostatectomy.
Original Articles. I. Uberetagoyena-Tello de Meneses, et al. Partial nephrectomy has become the standard surgical treatment for small renal masses under 7 cm, providing excellent oncologic and functional results.

domain: medical


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Lanvin's colourful S/S 2013 resort accessories
Autumn and winter is always a more sober time for fashion designers, but this year feels in many ways to have been more 'serious' than most. There's a decidedly mature look to a lot of the current accessory trends, and we reckon that by spring, we'll be more than ready for some fun and youthful colour.
Lanvin have got ahead of the game, releasing an explosion of colourful items for its pre-spring resort range. Featuring bags embellished with cutesy designs, neon accents and even sun visors - it's a world away from the darker looks that are dominating now. So if you're not looking forward to winter, check out what we can expect to see as an influence next spring!


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The Denver Broncos’ upset over the Carolina Panthers wasn’t the only surprise on Super Bowl Sunday. Two drugmakers doled out huge sums to advertise their brands in the third most-watched program in US television history.
Super Bowl 50 was pharma’s biggest turnout in three years. No ads appeared in 2014 and only one ran last year, according to iSpot.tv.
The ads, two branded spots developed by Valeant Pharmaceuticals and another unbranded spot from AstraZeneca, addressed what some critics panned as less-than-appealing conditions for such a broad audience. USA Today‘s annual Ad Meter ranked all three ads in the bottom five of this year’s Super Bowl crop.
Stephen Neale, executive creative director at healthcare agency AbelsonTaylor, said pitting pharma’s efforts against those of the auto, film and technology industries is an unfair comparison. “It’s always a difficult task to find a breakthrough idea that can hold its own with the rest of the Super Bowl ads,” he explained. “You need to create something that’s OPDP approvable and use emotion outside of humor to sell your brand.”
(The FDA’s Office of Prescription Drug Promotion regulates pharmaceutical advertising.)
George Belch, professor of marketing at San Diego State University, criticized the timing of the ads, saying “Given what you’re up against with all the humor, the celebrities and really big productions, I just don’t think it works that well,” according to the The Wall Street Journal.
An ad for Jublia, a toenail fungus treatment, made its second Super Bowl showing. Valeant Pharmaceuticals took a different creative approach for Super Bowl 50. Last year’s fully-animated spot featured a big toe tacking the floating words “toenail fungus” across a football field. This year it got the celebrity treatment, bringing in football legends Howie Long, Deion Sanders and Phil Simms.
The ad begins with a man seated at a bar celebrating a touchdown. As he raises his fists in the air, he hits a lamp attached to a wall shooting him into a spa-like oasis where Long, Sanders and Simms are dressed in white robes soaking their feet. The trio question the sandal-wearing intruder and his toenail fungus, saying: “How did he get in here? And with toenail fungus?” An animated big toe, sporting a Jublia-branded eye mask, swings in on a chandelier and proceeds to attack the floating words “toenail fungus” around the spa.
Valeant spent $4.9 million for the 30-second spot, according to estimates from iSpot.tv. The ad resulted in 68 million TV impressions.
Jublia launched in the end of June 2014. It generated $206 million in sales in the first nine months of 2015.
Valeant also plugged Xifaxan, its treatment for irritable bowel syndrome, employing an animated intestine in an ad called Football Game. During a bout of abdominal pain and diarrhea, the animated organ dashes to the bathroom only to stare down a long line. Two weeks later, after taking Xifaxan, the intestine struts back into a football stadium without fear of having to make a mad-dash mid game.
The drugmaker doled out $9.8 million for the 60 second spot, according to estimates from iSpot.tv. It resulted in 59 million TV impressions.
Xifaxan became part of Valeant’s portfolio last year when the drugmaker acquired Salix Pharmaceuticals. In the second and third quarters of 2015, Xifaxan brought in sales of $368 million. Valeant launched an integrated campaign for the drug on October 5.
AstraZeneca developed an unbranded spot to generate awareness for opioid-induced constipation. The ad, Envy, follows a man with the condition, showing his unease at how routine going to the bathroom is for seemingly everyone he encounters. As he watches a smiling man exit the bathroom, a voiceover tells viewers: “If you need an opioid to manage your chronic pain you may be so constipated it feels like everyone can go—except you.”
AstraZeneca and Daiichi Sankyo in March partnered to sell Movantik, an opioid-induced constipation drug, in a deal worth up to $825 million. AstraZeneca developed Movantik and Daiichi Sankyo paid $200 million upfront for to co-market the drug. Movantik brought in sales of $29 million in 2015. The drug was approved by the FDA in September 2014.
AstraZeneca spent $9.8 million on the 60-second spot—according to estimates from iSpot.tv—which resulted in 56 million TV impressions.


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What is better then picking up a happy dog from boarding or daycare? Picking up a clean and happy dog!
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domain: pet


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Declaring “we will not yield and we will not go quietly into the night,” Gov. Phil Bryant vowed to lead the fight against the U.S. Army’s recommendation to close the Reserve Officers’ Training Corps (ROTC) program at The University of Southern Mississippi.
Bryant made the remarks Friday during a news conference on the Hattiesburg campus outside of Southern Hall, where the university’s Army ROTC headquarters are located. He was joined by Southern Miss President Rodney D. Bennett and other dignitaries, including retired Gen. Buford Blount, a graduate of the program who led U.S. forces in toppling the regime of Iraqi dictator Saddam Hussein.
“We won’t accept this,” said Bryant, who has reached out to the state’s congressional delegation and others in the military community for assistance in maintaining the program. ‘We’ll continue to work through this at every opportunity.”
The Southern Miss ROTC program is one of 13 at universities across the country that the U.S. Army has targeted for closure as part of a restructuring that would move funding to programs at other schools.
Southern Miss currently has 87 cadets participating in the ROTC program which dates back to 1950. Of that total, five are scheduled to be commissioned as lieutenants in December with another 12 projected for commission next May.
Bryant and Bennett both said the program at Southern Miss has many graduates in its 60-plus-year history who have gone on to have stellar military careers, such as Blount, as well as success in civilian life. “As president, I intend to do all I can to see that this program continues,” Bennett said.
Bryant urged those attending the news conference to contact their federal representatives, including President Obama by e-mail at www.whitehouse.gov in support of keeping the Army ROTC program at Southern Miss.
“Simply ask the president, who will ultimately make this decision, to recognize the greatness of our cadets and their role in defending freedom around the world,” Bryant said.
For information about Army ROTC at Southern Miss, online visit http://www.usm.edu/army-rotc.

domain: politics


text:
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Translationally Controlled Tumour protein is a multifunctional calcium binding protein which has an important role in apoptosis, calcium levels balance and immunological response. The aim of this study was to evaluated the presence and distribution of TCTP in healthy human corneas and to identify and characterize the presence and distribution of this protein in human normal cornea. Since recent studies suggest that apoptosis, calcium levels and immunological mechanisms play a role in the pathogenesis of herpetic stromal keratitis, we studied TCTP expression in this disease.
We evaluated the expression of TCTP at both RNA messanger and protein level by using reverse transcriptase analysis, immunoblotting and immunohistochemistry in 10 healthy samples cornea: four obtained after penetrating keratoplasty and six from eyes enucleated for other pathologies. Finally, we analysed by immunohistochemistry ten paraffin-embedded samples of Herpes simplex virus keratitis collected at Siena Department of Human Pathology and Oncology: 5 had clinically quiescent disease and 5 had active corneal inflammation.
Reverse transcriptase and immunoblotting demonstrated TCTP expression in cornea as a 22,000 Da molecular weight band corresponding to the molecular weight of this protein. Immunohistochemically, all the layers of normal corneal epithelium showed TCTP cytoplasmic expression. TCTP was, also, observed in keratocytes and in the endothelium. In Herpes simplex virus keratitis samples, strong expression of TCTP was evident in stromal cells, in the inflammatory infiltrate and in neo-vessels.
In this preliminary study we demonstrated, for the first time, the presence of TCTP in human cornea, suggesting a potential role in the pathogenesis of herpes virus keratitis.
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/3306813447428149
Translationally controlled tumour protein (TCTP), also known as fortilin  or TPT1  is a ubiquitously expressed protein of 21kDa in mice and 23kDa in humans, expressed in all eukaryotes. It bears no sequence similarity with any other known protein . It is encoded by a gene that maps to chromosome 13q14.13 [1,3] and its expression is highly regulated at transcriptional and translational level and by a wide range of extracellular signals. Its name originates from the observation that TCTP transcripts accumulate in resting cells and are rapidly translated into the protein when the cells require it . Although TCTP was first considered a tumour protein , its expression is not limited to cancer, as it has been found in normal cells and tissues . Since its discovery by Yenofsky , it has become clear that TCTP is a multifaceted protein, implicated in many biological processes and exerting biological activity at extracellular and intracellular level . TCTP participates in cell growth, cell cycle progression, division and proliferation . An anti-apoptotic function of TCTP in human cancer cells has also been identified. This function may be related to calcium binding [9-12] and inhibition of Bax dimerization . The interaction between TCTP and p53 prevents apoptosis by destabilizing p53 . TCTP is also considered a heat shock protein with chaperone-like activity . It functions as an IgE-dependent histamine releasing factor, having cytokine-like activity in acute allergic response and being involved in immunological response . Association with a cytoskeletal component, F-actin, and a role in cell shape regulation were also recently discovered , as well as its capacity to bind tubulin and serve as a substrate for Polo-like kinase 1 (Plk-1) .
Ocular vision depends on corneal transparency and shape. These properties depend on the balance between cell proliferation and apoptosis, both mechanisms regulated by TCTP . The presence and distribution of TCTP in the human cornea has not yet been fully analysed. Studies in the literature have used proteomic techniques and been limited to cultured keratocytes . Here we evaluated the presence and distribution of TCTP in healthy human corneas for the first time by immunoblotting, reverse transcriptase analysis and immunohistochemistry. Since recent studies suggest that apoptosis , calcium levels  and immunological mechanisms  play a role in the pathogenesis of herpetic stromal keratitis (HSK), we studied TCTP expression in this disease.
Ethics approval for this study was obtained from the Institutional Review Board at the University of Siena (Italy). Informed written consent was obtained in all cases.
We used 10 samples of healthy corneal tissue, four obtained after penetrating keratoplasty (PKP) and six from eyes enucleated for retinoblastoma, in which the anterior segment was tumor-free. Ten other corneal tissue samples were collected from patients undergoing PKP for sequelae of HSK. The diagnosis of HSK was based on clinical and slit lamp findings. PKPs were performed consecutively from August 2010 to December 2011.
The surgical specimens obtained after PKP were grossly examined and halved (Figure (Figure1A-B).1A-B). Half of the samples were rinsed in sterile HBSS (Hanks balanced salt solution) at room temperature, blotted dry and dissected with a razor blade. To analyse protein and RNA, they were snap-frozen and stored in liquid nitrogen. The other half were fixed in 10% buffered formalin and embedded in paraffin. From paraffin-embedded blocks of PKP and enucleation samples, 4-μm-thick sections were obtained and processed for histology and immunohistochemistry. Anti-human TCTP mouse monoclonal antibody was prepared as previously described .
Frozen corneal tissue samples were thawed, reduced to small pieces with a razor blade and homogenized on ice with a Polytron blender (Kinematica, Lucerne, Switzerland) in lysis buffer [50mMTris–HCl, 5mM magnesium acetate, 0.2mM EDTA, 0.5mMdithiothreitol, 10% (vol/vol) glycerol, 0.2% (vol/vol) Triton X-100 (pH 7.5)] supplemented with a protease-inhibitor cocktail (Sigma Chemical Co, Milan-Italy), three times for 20 seconds each. Tissues were centrifuged at 750 x g for 10min at 4°C and the supernatant assayed for total protein content and stored at −80°C. Western blot analysis was carried out as previously described .
Total RNA was extracted using the method of Chomczynski and Sacchi. Frozen tissues were crushed with a pestle and homogenized in Tri-Reagent (Life Technologies, Monza-Italy) with a Polytron blender (Kinematica, Luzern-Switzerland). Cell pellets were thawed and immediately lysed with Tri-Reagent. RNA extraction was carried out as recommended by the manufacturer. TCTP mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) as previously described . The products were separated by 2.0% agarose gel electrophoresis, visualized by ethidium bromide staining, and photographed.
Immunohistochemistry was performed using the alkaline phosphatase–anti-alkaline-phosphatase (APAAP) method. Sections were dewaxed, rehydrated and washed in Tris-buffered saline [TBS, 20mMTris–HCl, 150mMNaCl (pH 7.6)]. Antigen retrieval was carried out by incubating sections in sodium citrate buffer (10mM, pH 6.0) in a microwave oven at 750W for 5min. Sections were incubated with an anti-human TCTP monoclonal antibody diluted 1:100 in TBS overnight at 4°C. Slides were washed and incubated for 30min with a rabbit anti-mouse antibody (Dako, Milan, Italy, dilution 1:30) and finally incubated for 30min with APAAP complex (Dako, dilution 1:50) in TBS. The alkaline phosphatase reaction was detected using fuchsin and naphthol. TCTP expression was evaluated semiquantitatively by HSCORE, as previously described . Placental tissue served as external positive control .
Statistical analysis was performed using a statistical software package (SYSTAT-7). Differences in TCTP pattern between cases grouped according to inflammation and neovascularization (groups 1 and 2) were analysed using the Chi-square test and Fisher’s exact test. Statistical significance was set at P≤0.05.
To evaluate steady-state levels of TCTP mRNA, total RNA extracted from tissues was examined by RT-PCR. When amplification was carried out in the presence of human TCTP primers, an intense band corresponding to the TCTP product was obtained on the agarose gel from the cDNA of each corneal tissue specimen (Figure (Figure22).
To further confirm the presence of TCTP in our specimens, Western blot analysis was carried out. After fractionation on SDS–PAGE, proteins of all specimen homogenates were blotted onto nitrocellulose and the filter exposed to an anti-TCTP antibody. A single band of the approximate molecular weight (MW) of 22,000Da was detected in all specimens (Figure (Figure3).3). Staining was abolished by probing blots identical to those described above with the antibody pre-absorbed with recombinant TCTP. Control tissue was represented by placenta, as previously described .
Immunohistochemical analysis performed with the anti-TCTP antibody showed cytoplasmic staining. TCTP expression was observed in the basal (Figure (Figure4A)4A) and intermediate (Figure (Figure4B)4B) cell layers of corneal epithelium with HSCOREs of 2 and 1, respectively. TCTP was also evident in keratocytes and endothelial cells with a HSCORE of 1 (Figure (Figure4B,4B, inset).
Of the 10 specimens from patients with HSK, 5 had active keratitis (moderate to severe inflammation and neovascularization) and 5 had quiescent disease (absent or mild inflammation). In the active HSK samples, intense and diffuse (HSCORE 3) TCTP staining was observed in stromal cells throughout all layers of the corneal epithelium (Figure (Figure5A-B).5A-B). In the quiescent disease samples, TCTP expression was weak (HSCORE 1), present only in corneal epithelium with little or no immunohistochemical expression in the stroma (Figure (Figure5A,5A, inset) (p=0.001).
TCTP is a highly conserved, ubiquitously expressed protein involved in multiple biological activities, including apoptosis . An extensive review of the literature showed that previous studies on TCTP in the cornea used proteomics techniques were limited to cultured keratocytes . In the present study we demonstrated the presence of TCTP in human corneal tissue by RT-PCR and immunoblotting, and confirmed these findings by immunohistochemistry using an antibody against TCTP. Since apoptosis plays a key role in HSK and is controlled by TCTP, we also attempted to find a pathogenic correlation between TCTP and HSK, evaluating TCTP expression in corneal samples with HSK. TCTP inhibited the proapoptotic proteins Bax and BCLxL, promoters of membrane attack complex (MAC) pore formation via dimerization of the mitochondrial membrane. TCTP inserts itself in the mitochondrial membrane, preventing Bax from dimerizing. This inhibits MAC pore formation and prevents any flux of apoptosis promoting factors into the cell. TCTP also binds to Mcl-1, stabilizing it and enhancing its anti-apoptotic activity. In addition, TCTP was shown to bind p53 and prevent apoptosis by destabilizing the protein and repressing transcription of p53 . A correlation was found between the degree of inflammation and expression of TCTP in corneal epithelium, as well as in inflammatory infiltrate in HSK patients with active disease . In the case of mild inflammation, we found low expression of TCTP, limited to neovessels and keratinocytes. These results suggest a double role of TCTP in herpes keratitis: as an antiapoptotic protein in corneal cells and as a cytokine-like protein in the stroma, where TCTP may be secreted by inflammatory cells, probably monocytes, in the extracellular milieu. Apoptosis has been shown to limit viral invasion: corneal infection with wild-type virus results in apoptosis of a number of infected epithelial cells . Primary herpes simplex virus-1 (HSV-1) corneal infection stimulates apoptosis of anterior keratocytes in the stroma underlying the site of epithelial injury. TCTP may modulate the keratocyte apoptotic response, limiting stromal damage as well as triggering the keratocyte apoptotic response via soluble mediators released by epithelial injury secondary to corneal HSV-1 infection. Considering the importance of apoptosis as a general mechanism for eliminating normal and pathologically altered cells, manipulation of apoptosis may offer new possibilities for treating HSK.
We also found a huge amount of TCTP in the extracellular stroma of actively inflamed HSK, which is in line with previous studies on broncho-alveolar lavage fluids of patients with eosinophilic pneumonia and asthma [27,28]. A mechanism by which extracellular TCTP might intervene through cytokine-like activity in the complex pathogenesis of HSK is by regulating the immune-mediated response to herpes simplex virus. In fact, HSK is currently thought to be an immune-mediated disease. CD4 positive Th1 cells are involved in the development of inflammatory HSK corneal lesions: infiltration of CD4 positive Th1 cells into the cornea causes release of cytokines, mainly interleukin (IL)-2 and interferon (IFN)-γ, which elicit an inflammatory response and recruit neutrophils and macrophages. TCTP is known to inhibit the release of IL-2 in human peripheral blood T cells and Jurkat T cells  and a role of TCTP in the modulation of T regs apoptosis/survival was recently demonstrated .
Repair of damage after each infection progressively impairs the biomechanical properties of the cornea. The repair process or wound healing of corneal epithelium is complex, including cell migration, proliferation and differentiation, all of which depend on calcium-mediated signals . Calcium concentrations vary during wound healing, thus inducing variations in cell motility, morphology and adhesion that are all essential to the process . Calcium levels are regulated by TCTP .
In this study we demonstrated the presence and distribution of TCTP in human corneal tissue and its expression in HSK, probably related to inflammation and neovascularization. These findings suggest a multifunctional role of TCTP in corneal wound healing, HSV-1 corneal infection, IgE-mediated hypersensitivity reactions and maintenance of corneal transparency and shape  by interaction with cytoskeletal proteins. Further in vivo and in vitro studies are needed to fully understand the pathophysiological roles of TCTP in corneal diseases, considering that only 20 samples were tested for TCTP and that no data on its role in HSK and other types of keratitis are available in the literature. Since a series of pharmaceutical compounds decrease TCTP expression by down-regulation of its expression at protein level, broader studies may help to determine whether TCTP may act as a target for drugs, contributing to new alternatives for the treatment of HSK, avoidance of complications and reduction of relapses .
TCTP: Translationally controlled tumour protein; PLK1: Polo-like kinase 1; HSK: Herpes stromal keratitis; PKP: Penetrating keratoplasty; HBSS: Hanks balanced salt solution; RT-PCR: Reverse transcriptase-polymerase chain reaction; APAAP: Alkaline phosphatase–anti-alkaline-phosphatase; TBS: Tris-buffered saline; MAC: Membrane attack complex; HSV-1: Herpes simplex virus-1; IL: Interleukin; IFN: Interferon; MW: Molecular weight.
The authors declare that they have no competing interests.
CB and MRA wrote the paper; BJR analysed the histological and immunohistochemical sections; FA conducted the molecular biology experiments; GMT acquired clinical data; JCS contributed reagents; MC contributed his expertise and fruitful discussion; SAT coordinated the work and gave final approval of the version to be published. All authors read and approved the final manuscript.

domain: literature


text:
Cost Involved in Selling Your Home
There are various expenses associated with selling a home. Some fees are customarily paid by the seller, some by the purchaser and some are negotiable. It is a good idea to have a knowledgeable agent that can guide you through those different paths. We can discuss in further detail the specific items at the time you list your property and walk you through other negotiable fees when a potential buyer submits a contract to make sure you are getting the best terms possible throughout the transaction.
Below are some of the costs you can expect to pay as the seller.
All mortgages on your home must be paid off will need to be paid off, this includes lines of credit. You are not required to pay this prior to closing but it will be deducted at the time of closing. Most mortgage statements reflect the principal balance of your loan. This is not a total payoff amount. There are fees and interest that have to be calculated into the final amount. Every lender has different fees that are charged and each mortgage is different so it is important to keep in mind there could be charges associated with your payoff that are not being disclosed on your monthly statement.
This could range from a shortage in your escrow account, current months interest amount, release fees and possibly a pre-payment penalty. We can help you understand the payoff process and can usually give you a good idea of what your final payoff is.
Outstanding Liens and Judgments
In some scenarios there could be a lien on your home. For whatever reason such as a child support judgement, tax lien or mechanics lien. In any case any of those items will also need to be paid from closing to clear the title for the new owner. If you are curious as to any liens that you might have on your home we can easily have our title company run an O&E (Owners and Encumbrances report).
This report can be helpful to make sure nothing is clouding title that might be an issue later. If there is something that needs to be paid off, a final payoff statement is ordered so that the lien can be released similar to a mortgage payoff.
Title Insurance and Escrow Fees
The title company will also be the closing company or escrow company. They have two responsibilities. Their first goal is to act as a disinterested third party who will conduct the closing, working with the buyers lender to calculate both sides of the transaction, collect all monies due and disburse the funds to the appropriate parties.
This includes sending you lender the payoff funds and any other creditors. They charge a $250 real estate closing fee which is usually split between the buyer and seller. There will be charges for administering your payoff and sending the funds to your lender. They will also have to file the release with the county. Their other objective is to issue insurance to the new owner and lender for clear title.
The fees they charge for the insurance is based on the sales price of the home and whether or not you have had a policy written within the last five year period. Each title company has filed rates and we can further examine those at the time of listing.
Real Estate Commissions
The seller is typically required to pay the real estate commissions. This fee is an amount that the listing and buyer agents spilt. The commission rate is negotiable and we can discuss your options at the time of listing.
It is customary that many times a buyer may submit an offer asking the seller to pay a portion of their loan costs. Typically it ranges from 1 to three percent of the loan amount and is a negotiable item. This is quite common especially with the amount of VA loans in this area.
The government does not allow the veteran to pay some of the fees so the seller is asked to assist with some or all of them. This again is calculated at closing and will be credited to the buyer at closing if an agreement to pay those costs is reached in the contract.
Every situation is different but some of the additional fees we encounter are items like home warranties, recording fees, wire or overnight fees. When discussing your particular circumstance and actual offers received we will be able to give you a detailed breakdown and are able to provide you with a seller net sheet prior to closing with an estimation of the costs involved with your particular transaction.

domain: finance


Putting the Private in Public Private
Opponents of the $1.5 billion State Center project suffered a setback Monday night when the House of Delegates passed a bill to “better define” public-private partnerships. The bill, backed by Gov. Martin O’Malley, carried an amendment seemingly aimed at scuttling a lawsuit against the State Center project.
The bill, and the floor amendment introduced in a work group composed of members from the Environmental Matters, Appropriations and Health and Government Operations committees, caused a stir all weekend. Opponents, including Del. Luiz R.S. Simmons (D-Montgomery), on Saturday said the amendment made the bill a “Trojan horse for the worst kind of special interest.”
The amendment retroactively allows the defendants in cases involving public-private partnerships (triple p’s, in the jargon) to remove the case directly to the state’s highest court—the Court of Appeals.
A coalition of downtown office building owners and Little Italy restaurateurs has sued the state over the State Center project, claiming the developers were chosen without proper bidding and that the cost to state agencies—$33 per square foot—is a bad deal for taxpayers. The state has dragged its feet in providing the documents the plaintiffs have demanded. The judge has sided with the plaintiffs on that point—called discovery. The house bill, if passed by the Senate, could short-circuit the discovery process, according to Ramsey Flynn, who has spoken for the plaintiffs in the case. He blasted out an e-mail quoting the plaintiffs’ lawyer, Alan Rifkin:
“The amendment is a transparent attempt to end-run a pending case and divest the court of jurisdiction in the midst of discovery and before a trial on the merits. It undermines the integrity of the judicial process and is improper from every perspective.”
Proponents said the retroactive provision was not nefarious or unusual, and that it is in the public interest to expedite the slow-moving court process because, well, time is money.
Here’s a link to a video of the House debate from Saturday.
While the amendment caused a ruckus, the main bill has been less controversial. The Washinton Post has the broadest take on the bill on Saturday, linking the effort to a little-noticed, O’Malley Administration trend toward more PPPs:
Under current regulations, only Maryland’s Transportation Department has the authority to pursue public-private partnerships. The measure would expand that power to the state’s Department of General Services — the umbrella agency responsible for nearly all procurement, buildings and construction.
It would allow the state to lease public assets for up to 50 years and would set up a process for companies to make unsolicited proposals to take over management of state facilities.
In exchange for constructing roads or buildings or upgrading existing ones, the state would guarantee investors a stable return or free them to operate the facilities as for-profit businesses if they share with the state a percentage of earnings.
PPPs and long-term leases have been a trend in government for several years. The deals are often used to get politicians out of a short-term fiscal jam when facing re-election or a jump to higher office, but they are not always great for taxpayers.


text:
Introduction to Belle
Belle (벨; formerly known as Lucky) is a South Korean singer under C9 Entertainment. She is a member of the pre-debut girl group C9 GIRLZ and a former member of GOOD DAY and their Morning unit.
She is Filipino-Korean.
Her role model is Girls' Generation's Seohyun. Lucky was
a participant in the survival show “The Unit”.
Fun Facts About Belle
|1.||Her nickname is Rocky (GOOD DAY “Pops in Seoul”).||
|2.||Belle was a participant in the survival show “The Unit” (ranked 24th).||
|3.||Belle’s role model is SNSD’s Seohyun.||
|4.||Belle likes Disney.||

domain: celebrity


text:
CHESTER >> Wednesday the city of Chester greeted Chum Bun Rong, the current Ambassador of Cambodia to the United States, for a tour of the city with which he said he was “very impressed.”
First on the docket was a visit to the City Council chambers and an address to the mayor and council before hosting an informational section on his home country. Later came lunch at Uno’s, a reception at the Wharf Building on the waterfront and finally box seats at the Philadelphia Union game against the Columbus Crew.
Bun Rong’s visit was a part of an economic development and Sister Cities initiative between Chester and the Cambodian capital of Phnom Penh.
“If we have investment, it will generate jobs, labor ... I want (Cambodian) investors and businessmen to come here and invest in this city, so that we can enjoy together the prosperity of people,” Bun Rong said.
Among the top exports in the country is knitted or crocheted clothing, a $7.1 billion boon for the country. Garments account for 80 percent of all exports for the country.
Bun Rong said that through wars and genocide — between 1.3 and 3 million people died from 1975 and 1979 under the oppression of the Khmer Rouge — before the country was invaded and occupied by the Vietnamese from 1977 until 1991.
Many fled the country during the mass genocide, some of whom established a Cambodian community in South Philadelphia.
However, economic growth, from when Bun Rong described as a “business-friendly” government, and the increase of exports and tourism monies, the country is eager to establish greater trade relationships with the United States and Europe.
“My country is now peaceful and we need to cooperate with other counties,” Bun Rong said. “We need to work together ... I hope that the American people from here will visit my country and vice versa.”
“I will bring my people here so they can see the same project in other countries.”


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THE oil and gas sector is becoming increasingly important to the North East economy as firms in the sector benefit from a raft of new business wins, a new study suggests.
Research by NOF Energy indicates that the oil and gas sector is enjoying a resurgence in the region, especially as the UK’s industry capital, Aberdeen, struggles to keep up with demand.
NOF Energy, the business development organisation for oil, gas, nuclear and offshore renewable sectors, says its study also demonstrates how the industry and its supply chain is well-positioned to capitalise on any business opportunities as they emerge.
The industry supports at least 440,000 jobs across the UK, 15% of which are based in the North East, equating to 66,000 workers.
There are thought to be around 1,500 supply chain companies in the region which supply the sector while the 200 NOF Energy members in the region have a combined turnover of £2.5bn. Of these, 50 are involved in the subsea sector and have a combined turnover of over £1bn, supporting 10,000 jobs. And far from being a sunset industry, the UK oil and gas industry – which currently satisfies 73% of UK energy demand – has the potential to satisfy close to 50% of UK demand in 2020 if the current rate of investment is sustained.
Chief executive George Rafferty said: “Research among our member companies shows how robust the oil, gas and offshore industries are in the region and how they are making a significant contribution to the economic wellbeing of the North East.”
He cited a string of recent contract wins, profit announcements and successful projects including:
Exports at BEL Valves, of Newcastle, now account for more than half of the company’s £60m turnover with projects in Africa, Australia, the Gulf of Mexico, the former Soviet Union, and the Far East.
Nortech Solutions, based in Stockton, has been awarded a £2m contract by EnQuest, of Aberdeen, to extend the operating life of an offshore North Sea platform.
Houghton International, of Newcastle, an electro-mechanical engineering specialist, has completed its largest project on behalf of a leading international provider of offshore contract services for oil and gas wells.
Mech-Tool Engineering, a global fire, blast and noise protection products manufacturer, has celebrated one of the most successful years in its 43-year history. Due partly to winning and delivering a major project off the coast of Russia’s Sakhalin Island, the Darlington firm recorded turnover in excess of £16m and profits of £600,000.
Revenues at IHC Engineering Business, of Stocksfield, Northumberland, will grow by 25% this year while its workforce will increase by 30 thanks to the oil and gas sector. It is working on two major pipeline lay towers, which are taller than the Tyne Bridge, and which will take more than 400,000 man hours to complete.
Mr Rafferty added: “The oil, gas and offshore sector, which supports thousands of jobs and generates much- needed income for the region, also has the capability and potential to exploit any emerging new markets and opportunities.
“This is good news for the region, its economy and future job creation.”


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'A Friends House' with a cliffside ocean view is located in the eco-luxurious area of Redonda Bay. South facing, sweeping views with Costa Rica in the distance. Private infinity pool awaits you on the large deck with built in BBQ. This gorgeous vacation home offers two master suites, ideal for 2 couples or a small family. Located next to Aqua Wellness resort the property offers access to a private bay, white sand beach and restaurant and bar. Stand up paddle boards, kayaks, snorkeling, daily yoga and spa services are all available. Nearby Gigante offers fishing, sailing, and surfing and other restaurant options. Abundant wildlife with Monkeys in the tree canopy above. Neighbors to Mukul Resort with a world class Spa and golf course. Discover Nicaragua- you will not be disappointed!
- Half Baths0
- Min Stay3 nights
2 Bedrooms, 3 bathrooms, sleeps 4-6
- Response rate:
- Response time:
- within a few hours
- Calendar last updated:
- Aug 2, 2018
Pool / Spa
Local Services & Businesses
Sports & Adventure Activities
Check-in: 10:00 AM
Check-out: 12:00 PM
100% refund if canceled at least 30 days before arrival date. 50% refund if canceled at least 14 days before arrival date. You must contact the owner directly to request cancellation.
Spring Break Trip away from the Snow
The home located within the gated community of the Aqua Wellness Resort is a beautiful well appointed home with an amazing view of the ocean and cliffs below. There is no access to the ocean directly below the home since it is high on a cliff, but a beautiful private sand beach is just a 5 minute walk The home has everything you need for your stay, however, if you would rather not cook, the Aqua Resort restaurant is just a short walk. The fishing town beach of Gigante is a short walk or drive as well as Rivas town and San Juan del Sur make for a fun day trip, although these beaches were very busy with locals due to the Easter Holiday. Playa Poyoyo is a beautiful less busy surf beach a short drive away. The home is so beautiful and maintained to perfection, both inside and outside. We highly recommend this property. Our travels to Nicaragua included a few days on Lagona Apoyo in the lake region and a few days at this home on the beach. We rented a car and had a fabulous trip, never feeling unsafe. We are Wyoming residence and highly recommend Nicaragua and this home.
The house is great and the location is nothing short of spectacular. From the back of the house, you overlook the pool and spacious patio and deck to cliffs overlooking the waves of the Pacific crashing below. In the distance are some small islands and in the far distance, you can see Santa Rosa National Park in Costa Rica. Bedrooms are spacious and comfortable. Bathrooms are also spacious and comfortable with double sinks in both master suits and large showers. Kitchen is large and well stocked with supplies. A 5 minute walk down a big hill gets you to Playa La Redonda at Aqua Wellness Resort. A fabulously beautiful crescent beach ringed with rocky cliffs. Sunsets are goregous. Food at the resort restaurant is good but pricey. We had a great time and would go back in a heartbeat.
Perfect house. Stunning view.
Could not ask for a better house to stay in. It is equipped with everything you need including a pool with a stunning view.
First Time Nicaraguan Visitor
We had the pleasure of staying in this wonderful ocean front home just after Thanksgiving. We stayed with one other couple and had the most relaxing visit. The house is beautiful, fully outfitted with everything one needs, and the deck area and swimming pool are gorgeous. We cooked dinners at home a couple of times, but also enjoyed area restaurants within easy walks and drives. None of us spoke Spanish, but we were easily able to negotiate this friendly area. We like to hike, read, and go birding; our friends like to swim and surf. We all were able to do exactly what we wanted. Very happy with our stay.
Central America explorer
We stayed at this fantastic home with our family of 4 over the holidays. The views from the back deck and pool are breathtaking by day and by night the stars are more brilliant than I have ever witnessed. You have the proximity of yoga just a short walk away with a restaurant that serves fresh local seafood. You can keep your stay quite and soulful or explore the local surfing and restaurants a short walk or drive down the road. We surfed, kayaked, paddled boarded, hiked and fished with a local and wished we would have been there for two weeks instead of one. The home is furnished perfectly and you have everything you need to make your stay comfortable and cook at home if so inclined. This part of Nicaragua is a treasure and I encourage you to take advantage of this great location and well-appointed home.
Beautiful home with amazing view
If you're looking for a beautiful tropical getaway, then you have found the spot. The ocean views from this home and it's pool area are absolutely breathtaking! You're likely to find a monkey hanging out close by enjoying the view with you! Inside the villa you will find it to be spacious and beautifully furnished with many lovely pieces reflecting local craftsmanship.The kitchen is well equipped for cooking in or enjoying a BBQ by the pool. Just a short walk below is a protected beach cove at the Aqua Resort. We enjoyed their rental activities such as kayaking, paddle boarding, and snorkeling, as well as, daily yoga classes on a beautiful platform overlooking the sea. We also popped down to their beachfront restaurant and bar deck for some delicious bites. The hike back to the house will keep you fit.
Holiday rates are $350.00 per night which include Thanksgiving week, Christmas, New Years and Easter (Semana Santa)

domain: travel


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The CMA develops and delivers a fulsome range of accredited continuing professional development programs, designed to help physicians and whole health care teams grow their skills and impact.
One of the most impactful ways to advance health care team’s knowledge is through CMA’s Physician Leadership Institute Team Training. Tailored to your needs and delivered on your timeline, our expert team of faculty will deliver accredited courses that can help take your team’s skills to the next level.
We offer over 30 engaging courses in English and 5 essential courses in French, all aligned to the LEADS in a Caring Environment framework:
- Lead self
- Engage others
- Achieve results
- Develop coalition
- Systems transformation
Courses can be delivered to teams as small as 10 to as large as 40, enabling system and culture-wide change in your organization.
NEW OFFERING – We are also very pleased to let you know about our new self-paced learning program Leadership Essentials – free and accredited courses that can be completed in an hour or less, any time. Whether you are looking to build a leadership foundation or brush up on the fundamentals, these courses can benefit physicians in any specialty or practice.
The modules include:
- Building self-awareness and personal mastery
- Communicating effectively
- Practical strategies for engaging health care teams
- Foundations of physician wellness
Please feel free to share with your teams or any of your contacts whom you feel may benefit from these offerings.
Learn more and connect with CMA’s Physician Learning advisors:


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The 90s offered a lot of great music and sometimes picking out the best tracks is not that easy. However, here are a few that are definitely worth considering in any shortlist of the best that the 90s have to offer.
My Name Is
This is the first hit from Eminem and most would argue that he never produced anything quite like this again. The song takes the listener into the world of the artist which might not be a pretty place to be, but it is definitely memorable.
Despite all the hits of the 80s given to the world by Madonna, by the time that the 90s arrived she was at her peak. Vogue took the listener into a world that blended the 90s with old-time Hollywood and the infectious dance was something that could be seen on dance floors everywhere.
Oasis epitomised 90s music for many people and this song is considered to be their first real classic. The lyrics are to the point and the song set the scene for the rest of their hits throughout the decade.
This is a serious look at the class divide from Pulp, right in the middle of the decade when musicians were making their voices heard on social issues. The song is based on a real conversation Jarvis Cocker had with a fellow student back in the 80s. Subsequent efforts by journalists to track down this woman have so far failed although there are rumours as to her identity.

domain: artistic


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Marvin Lewis was sentenced Wednesday in Austin for crimes during 2014 and 2015. Prosecutors say the 40-year-old Lewis, who lived in Austin and Houston, targeted jewelry stores in Texas and Ohio.
Jurors in June convicted Lewis of 25 counts including conspiracy, interference with commerce by threats or violence, money laundering and firearms-related charges.
Investigators say the heists included $548,000 worth of diamonds stolen from a jewelry store in Strongsville, Ohio. Other robberies or attempted holdups were in Austin and the Houston area.
A co-defendant, 38-year-old Brandon Grubbs of Houston, pleaded guilty to conspiracy and to a firearms count and was sentenced to 15 years.
Copyright 2017 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed

domain: law


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Jan 10, 2014, 11:08 AM EST
- Tottenham have scored nine goals in four league games under Tim Sherwood; the same number that they scored in the last 10 games under Andre Villas-Boas.
- Crystal Palace are unbeaten in four previous Barclays Premier League games at White Hart Lane (W1 D3).
- Since Tony Pulis has taken over, Palace have conceded just eight goals in eight league games (three clean sheets). They conceded 21 in the first 12 of the season (two clean sheets).
Tottenham manager Tim Sherwood will have his four match unbeaten record tested when Crystal Palace visit White Hart Lane on Saturday (Watch live on NBCSN at 10am ET, online via NBC Sports Live Extra).
Spurs have looked a new club since the sacking of Andre Villas-Boas following the 0-5 home loss to Liverpool on December 15th. Chairman Daniel Levy is looking quite shrewd in his decision to hand the managerial reins to Sherwood, who has instilled his side with a new found sense of expression.
Under AVB, Tottenham was an overly-coached squad that was too preoccupied with complex tactics that the player’s lost their sense of freedom and creativity. Under the guise of Sherwood however, Spurs have adopted a laissez-faire attitude that’s bringing out the best in the players.
After going to Southampton and winning 3-2 on December 22nd, Spurs returned home to salvage a tough 1-1 draw with West Brom. They found their feet again in the 3-0 blitz of Stoke City before earning a profound 2-1 victory against Manchester United at Old Trafford.
With all the talent in the world, Sherwood’s loose system was exactly the tonic Spurs needed to get back on track. In time, more purposeful play will need to be installed but for now Spurs are simply enjoying their football. Part of that enjoyment, of course, involves beating clubs who you should beat. Prime example – Crystal Palace.
The Eagles have improved dramatically under Tony Pulis and are no longer the Premier League’s whipping boys (that honor is firmly in West Ham’s hands). Sitting in 18th place one point shy of safety, a win on Saturday could see the South London club move out of the relegation zone and leapfrog Fulham and Cardiff.
One of Palace’s keys to success under Pulis has been their improved away form – they’ve now have won three in six on their travels after losing the previous six in a row. Adding to the uplift, of the Eagles’ three away losses in that run, two have been to Manchester City and Chelsea.
Injuries could play a role in Saturday’s outcome with Spurs having a laundry list of players on the trainer’s table. In the defense, Jan Vertonghen (ankle) and Younes Kaboul (thigh) are long term absentees while Kyle Naughton (hamstring) is also out. In the midfield, Sandro (calf), Lewis Holtby (knee), Andros Townsend (hamstring) are all possibilities to return while Gylfi Sigurdsson (calf) is doubtful. Up front, Jermain Defoe (hamstring) will be a late fitness test while Roberto Soldado (calf) is a doubt.
What They’re Saying
Spurs midfielder Christian Eriksen: “We’ll probably have the ball more, but we just have to use the ball well against what could be a defensive team. Every game is difficult but I believe we can maintain our good run of form in the league and pick up another win.”
Palace manager Tony Pulis: “Watching the team win away from home gives you confidence because although you need to win your home games, we have the same amount of away games coming up. To be able to go into those games knowing that you can get points away from home is important.”
With Tottenham’s rash of injuries, this is no lollypop match. I expect Spurs to win but think this will be a tight affair with only a goal separating the sides.
La Liga and Serie A roundup: Valencia, Napoli falter in Champions League bids; Milan derby a stalemate
Nov 23, 2014, 10:20 PM EST
Catch up on all of Sunday’s action and movement in La Liga and Serie A.
Nov 23, 2014, 8:15 PM EST
The Revs and Galaxy may both hold one-goal advantages, but they’re very different leads built in very different ways.
Nov 23, 2014, 7:45 PM EST
The Crystal Palace manager hailed his side’s “Neil Warnock performance” as they pull themselves out of the relegation zone.
Nov 23, 2014, 7:14 PM EST
The Galaxy find themselves just 90 minutes from the third MLS Cup final in four years after a narrow victory over the Sounders.
Halftime, MLS Cup Playoffs: LA Galaxy, Seattle Sounders in 0-0 Western Conference Championship stalemate
Nov 23, 2014, 6:05 PM EST
Zeroes on the scoresheet, but plenty of action in the first half. Sounders very patient, but Galaxy getting close.
Nov 23, 2014, 5:32 PM EST
He’s only ever done it twice in his MLS career, but with the season — and maybe his career — on the line, Thierry Henry will play on artificial turf next week.
Nov 23, 2014, 4:50 PM EST
The Spurs manager wouldn’t be drawn into talk about the winner from Christian Eriksen or the red card to Gaston Ramirez, instead discussing how he was just delighted to get three points any way he can.
Lineups, MLS Cup Playoffs: LA Galaxy host Seattle Sounders in Western Conference Championship, leg 1
Nov 23, 2014, 4:25 PM EST
Lineups are in for the Galaxy and Sounders’ Western Conference Championship first leg matchup. Two massive omissions leave Seattle shorthanded.
Nov 23, 2014, 4:07 PM EST
The Eastern Conference Championship first leg finished Red Bulls 1-2 Revolution, putting Jay Heaps’ side in full control of the series.
Nov 23, 2014, 3:10 PM EST
Steve Bruce challenged the decision to give Gaston Ramirez a straight red card, and criticized Tottenham defender Jan Vertonghen for going down too easily.
Halftime, MLS Cup Playoffs: New York Red Bulls 1-1 New England Revolution after thrilling first half
Nov 23, 2014, 2:52 PM EST
The first 45 at Red Bull Arena was wild. Take a deep breath and relive the thrilling actions, goals and all.
Nov 23, 2014, 2:00 PM EST
New England is undefeated in 11 straight matches with Jermaine Jones in the lineup.
Nov 23, 2014, 1:29 PM EST
Brendan Rodgers was quite downtrodden following Liverpool’s 3-1 defeat at Crystal Palace, the club’s third defeat in a row.
Nov 23, 2014, 12:55 PM EST
Hull led inside 10 minutes and looked solid on both ends, but a straight red to Gaston Ramirez for a kick at Jan Vertonghen, and Christian Eriksen completes the Spurs comeback a minute before full time.
Nov 23, 2014, 11:50 AM EST
Fastest ever red card? It certainly has a shout after Australian completes an ugly tackle straight after coming on.
Nov 23, 2014, 10:48 AM EST
Mauricio Pochettino has called on Ben Davies and five others to fix things for Spurs, as both sides make a whopping six changes to their lineups from last weekend.
Nov 23, 2014, 10:26 AM EST
Yannick Bolasie was a force down both flanks as Neil Warnock’s Crystal Palace pulls out of the relegation zone.
Nov 23, 2014, 10:01 AM EST
David Moyes began his tenure in Spain with a 0-0 draw, but there was more to it than that.
Nov 23, 2014, 8:19 AM EST
After missing the World Cup while rehabbing a serious ankle injury, Marco Reus is faced with yet another long road back.
Nov 23, 2014, 7:48 AM EST
With Daniel Sturridge shelved for weeks and Mario Balotelli unable to get past a groin knock, Liverpool turns to Rickie Lambert to produce goals at Selhurst Park.
- Inside Look, MLS Cup Playoffs: How Revs, Galaxy built very different one-goal leads 0
- Recap: LA Galaxy grab 1-0 lead over Seattle Sounders in Western Conference Championship 1
- Despite artificial turf, New York Red Bulls’ Thierry Henry says he’ll play next week at New England 1
- Recap: New England Revolution snatch 2-1 lead, two away goals from New York Red Bulls 6
- Video: Brendan Rodgers says Liverpool needs to “become a team” 1
- Crystal Palace 3-1 Liverpool: Bolasie carves up Liverpool defense 3

domain: sports


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Terms & Conditions
Half Moon Bay Limited (HMB) at Stephens Way, Bath Road, Warminster, BA12 8PE and The Ice House, 124 Walcot Street, Bath, BA1 5BG, Tel: (+44) 1225 473873, conducts business on its own terms and conditions. Any attempt to exclude these or vary them by a customer without written agreed consent by HMB shall be void and have no effect.
Placing an order with HMB does not constitute acceptance of that order by HMB. HMB may make part deliveries, giving prior notice when possible.
Orders and Carriage:
The minimum order is £150 (excluding VAT) and this qualifies for carriage paid within the mainland UK. Back orders are kept on our system unless instructed by the customer. Back orders are shipped when the value is £75 and over (excluding VAT). For back orders below £75, our sales team will contact the customer about increasing the order value. These orders will not incur a carriage charge. For details on orders outside of mainland UK please contact our sales team on 01225 473873 Option 1 or email email@example.com
Credit / Proforma Accounts:
Credit accounts are offered to customers subject to a credit check and are on strict 30-day payment terms from document date. Customers who fail to pay within their terms run the risk of having their credit limit removed. If payment is late by 45 days past the payment terms on the account, then all orders for that customer are automatically cancelled with no notification given. If Credit cannot be offered, then the account is set to proforma and payment must be received in full for the value of the goods in stock before the order is shipped. If payment is not received within 2 weeks of the proforma invoice being emailed and then followed up with a phone call, then the order is cancelled with no notification given.
Credit and debit cards:
All major credit and debit cards are accepted (with the exception of American Express). When using the Half Moon Bay website, no credit card details are required. We will contact you when the order is ready to ship detailing the amount to be paid if on a proforma account.
Credit card payment may be used by credit account customers to settle outstanding invoices. However, if payment is made after the 30 days due date, Half Moon Bay Ltd reserve the right to apply a charge of 1.5% of net invoice value to cover our processing charge.
HMB reserve the right to charge interest on late payments in accordance with the Late Payment of Commercial Debts (Interest) Act of 1998, as amended by the Late Payment of Commercial Debts Regulation 2013.
Damages, shortages and returns and faults:
Returned goods will only be accepted when authorised by HMB prior to the goods being returned.
Authorised returned stock must be in saleable condition, in the original packaging and have no price labels attached. Otherwise HMB reserve the right to charge a fee up to a maximum of 100% of the invoiced trade price of the item. Unauthorised returns will not be accepted. Credit claims for shortages or damages must be notified to HMB in writing within 14 working days of the receipt of goods. Credit claims for faults on products should be notified to HMB within 3 months of the receipt of the goods.
All credit claims should be emailed to firstname.lastname@example.org and must contain the following information: Company name, Invoice number, Product Code, Quanity, Reason for request & Photographic evidence.
Title and Risk:
Until HMB has been paid in full for all goods supplied, property in any such goods remain with HMB, although all risk passes to the customer at the time of delivery.

domain: economics


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Last Update: 02-03-2024
Processing time for Denmark Visa
15 to 21 Working Days
Denmark visa fees
Denmark visa validity
Denmark visa stay duration
How to apply for a Denmark visa
Documents required for Denmark visa
Planning a trip to Denmark? Navigating the visa process is crucial for a smooth and hassle-free journey. In this comprehensive guide, we'll walk you through the step-by-step process of applying for a Denmark Tourist Visa.
From understanding the visa requirements to submitting your application via Teleport or VFS Global Visa Application Centre, we've got you covered. Whether you're an Indian passport holder seeking adventure in Denmark or simply exploring the possibility, this guide will provide you with all the essential information you need to make your travel dreams a reality.
So let's dive in and embark on the journey to explore the captivating sights and experiences awaiting you in Denmark!
If you can't find what you're looking for, feel free to reach out to us if you have any queries or need assistance, our committed team is prepared to offer guidance and support.
Call us at +91 73148 52914 or reach out to us on WhatsApp
Get ready for a smooth journey to Denmark – Safe Travels!✈
A Denmark Tourist Visa, officially known as the Schengen Visa, allows travellers to visit Denmark for leisure purposes, such as tourism, sightseeing, or visiting friends and family, for a short duration of up to 90 days within a 180-day period. This visa also grants access to the other Schengen countries, making it an ideal choice for exploring multiple destinations in Europe.
Indians holding an ordinary passport need a Denmark tourist visa before departing for their trip to Denmark. However, Indians with Diplomatic or official passports do not need a visa for entering Denmark.
To apply for a Denmark visa through Teleport, follow these straightforward steps:
Step 1: Start Your Application
Visit Teleport's Denmark visa application portal and click on 'Apply for Visa' to begin.
Step 2: Provide Basic Details and Pay Fees
Upload scanned copies of your passport's front and back pages. You can either let Teleport autofill your information or enter it manually. Pay the visa fees through Teleport, choosing between full or partial upfront payment.
Step 3: Gather Required Documents
Teleport's relationship manager will help you collect all the necessary documents for your Denmark visa application.
Step 4: Schedule Your Appointment
Teleport's relationship manager will set up an appointment at the VFS visa application centre for you, based on your preferred location and dates.
Step 5: Visit VFS Centre and Submit Biometrics
Arrive at the appointed time at the nearby VFS Visa Application Centre to submit your biometrics and required documents. It's recommended to arrive at least 30 minutes early to avoid any issues.
After VFS forwards your documents to the consulate for review, they will make a decision and return the documents to VFS.
You can choose to have your passport delivered to your address or collect it in person from the VFS visa centre.
If you're applying for a Denmark visa directly through VFS Global Visa Application Centre in India, follow these steps:
Step 1: Determine Your Visa Type
Identify the appropriate visa type for your trip and ensure you meet the eligibility criteria. Familiarise yourself with the required documents, processing time, and fees.
Step 2: Gather Required Documents
Collect all the necessary documents for your Denmark visa. Refer to the document checklist for specific details.
Step 3: Begin Your Application
Fill out the online application form after gathering all required documents. For short-term visas, complete the form via ApplyVisa.
If you are applying for a residence permit, Work or Study visas please use the online application form available on NewToDenmark
Step 4: Book Your Appointment
Schedule an appointment at the VFS Visa Application Centre for biometric data collection after submitting your visa application.
Upon booking, you will receive a confirmation email along with your appointment letter.
If you're applying as a family or group, remember that each member must schedule their individual appointments.
Step 5: Pay Your Fees
Complete your appointment's service fees payment online before heading to the Visa Application Centre.
Step 6: Visit the Visa Application Centre
Print the appointment letter and payment fee receipt after completing your online application and payment. Submit your fully completed visa application form and documents at the designated Visa Application Centre.
|Operating Hours (Mon to Fri)
09:00 - 15:00
09:00 - 13:00
09:00 - 14:00
08:00 - 15:00
09:00 - 13:00
08:00 - 15:00
09:00 - 14:00
09:00 - 14:00
08:00 - 15:00
09:00 - 16:00
08:00 - 14:00
Step 7: Track Your Application
Use the VFS online application tracking tool to monitor the status of your application by providing the reference number issued to you and your date of birth.
Step 8: Collect Your Visa
Retrieve your passport from the Visa Application Centre once your visa application is processed. Bring the necessary documents for collection or authorise someone to collect it on your behalf.
For specific collection options and hours, contact the Visa Application Centre during their opening hours.
Must carry this letter with you on the day of your appointment.
Visa Application Form
Sign and bring the completed form with you on your appointment.
Visa Fee Receipt
Print the visa fee payment receipt
Covering a minimum of 30,000 Euros for the entire stay in the Schengen area.
Proof of Accommodation
Confirmed hotel reservations covering your travel dates, including reservations for other Schengen countries if applicable.
Proof of Internal Travel
Proof of round-trip flights or intra-Schengen travel.
ITR (Income Tax Return)
Include ITR acknowledgment for the last two assessment years.
Certificate of company registration.
If a Minor (under 18 years)
If Student (aged 16+)
Marriage Certificate or copy of ration card if applicable.
It's advisable to apply for your Denmark Tourist Visa well in advance of your planned travel dates. The earliest you can apply is up to six months before your intended travel date. However, it's recommended to submit your application at least 45 days prior to your departure to allow sufficient time for processing.
The recommended financial requirement for a Denmark visa application in India is typically DKK 350 per day per person(if staying in a private home) or DKK 500 per day per person (if staying at a hotel). This amount should cover your intended stay duration, including accommodation, food, and other expenses while in Denmark. It's important to ensure that your bank statement reflects this level of funding to demonstrate your ability to support yourself during your visit.
VFS Appointment Fees
Euro 20 (INR 1,825/-)
Embassy Fees (0-6 years)
Embassy Fees (6-11 years)
Euro 40 (INR 3,400/-)
Embassy Fees (12+ years)
Euro 80 (INR 7,100/-)
In conclusion, obtaining a Denmark Tourist Visa involves a systematic process that ensures your journey to this beautiful Scandinavian country goes smoothly. Whether you choose to apply through Teleport or directly at the VFS Global Visa Application Centre, it's essential to adhere to the outlined steps and meet the necessary requirements.
Remember to plan ahead and apply for your visa well in advance to allow for ample processing time. Additionally, ensure you fulfil the financial obligations required for your stay in Denmark, as this plays a crucial role in the visa application process.
By following this comprehensive guide and being prepared with all the required documents and information, including the Denmark visa for Indians, you'll soon find yourself ready to embark on an unforgettable adventure in Denmark.
If you have questions or need help, our dedicated team is here for you. Call us at +91 73148 52914 or message us on WhatsApp.
Safe travels and enjoy your Danish adventure!