FBI testing of the gun used within the fatal shooting on the film set of “Rust” discovered that the weapon dealt with by actor Alec Baldwin couldn’t be fired with out pulling the set off whereas the gun was cocked, in accordance with a newly launched forensics report.
Baldwin had the gun whereas rehearsing a scene of the Western movie on the Bonanza Creek Ranch in New Mexico in October when a shot fired, killing cinematographer Halyna Hutchins and injuring director Joel Souza.
In December, Baldwin informed ABC News he by no means pulled the set off of the gun that shot Hutchins. “The set off wasn’t pulled. I didn’t pull the set off,” Baldwin stated.
An lawyer for the actor informed CNN on Sunday the FBI report “is being misconstrued.”
“The gun fired in testing just one time – with out having to drag the set off – when the hammer was pulled again and the gun broke in two totally different locations,” lawyer Luke Nikas stated in an e mail to CNN. “The FBI was unable to fireside the gun in any prior check, even when pulling the set off, as a result of it was in such poor situation.”
“The important report is the one from the health worker, who concluded that this was a tragic accident,” Nikas stated. “That is the third time the New Mexico authorities have discovered that Alec Baldwin had no authority or information of the allegedly unsafe circumstances on the set, that he was informed by the particular person accountable for security on the set that the gun was ‘chilly,’ and believed the gun was protected,” Nikas informed CNN.
Within the abstract of the postmortem investigation into Hutchins’ demise, which was formally signed by the New Mexico chief medical investigator, the reason for demise is listed as “gunshot wound of chest,” and the style of demise is listed as an “accident.”
“Overview of obtainable regulation enforcement stories confirmed no compelling demonstration that the firearm was deliberately loaded with stay ammunition on set. Based mostly on all accessible data, together with the absence of apparent intent to trigger hurt or demise, the style of demise is greatest labeled as accident,” the report concluded.
Baldwin, within the ABC Information interview, additionally described cocking the gun as he talked by the scene with Hutchins. “So then I stated to her, ‘Now on this scene, I’m going to the gun.’ And I stated, ‘Do you wish to see that?’ And he or she stated, ‘Sure.’ So I take the gun and I begin to cock the gun. I’m not going to drag the set off.”
Cocking a revolver pistol just like the one used on the film set entails pulling the hammer of the gun again to organize the weapon to fireside. When the hammer of the gun is launched ahead with sufficient power – as occurs when the set off is pressed – it strikes the primer of a spherical of ammunition inflicting the gun to fireside.
The FBI forensic report was handed over to the Santa Fe County Sheriff’s Workplace as a part of the continued investigation into the deadly on-set taking pictures.
The report discovered that the gun, a .45 Colt (.45 Lengthy Colt) caliber F.lli Pietta single-action revolver, “couldn’t be made to fireside and not using a pull of the set off” with the hammer cocked on the ¼ and ½ positions. It additionally discovered that when the weapon was absolutely cocked it “couldn’t be made to fireside and not using a pull of the set off whereas the working inner elements have been intact and useful.”
FBI examiners noticed an inner malfunction of the gun throughout testing on the absolutely cocked place, with the report noting “parts of the set off sear and cylinder cease fractured whereas the hammer was struck.”
The FBI report famous the constraints of the forensics testing, saying “it is probably not potential to recreate or duplicate the entire circumstances which led to the discharge of a firearm and not using a pull of the set off.”
An lawyer representing Hannah Gutierrez Reed, who served because the armorer and props assistant on the movie, stated the forensics report indicated “Baldwin needed to have pulled the set off to fireside the revolver” and that the 24-year-old was getting used as a “scapegoat.”
A part of the Santa Fe County police investigation focuses on how a live round of ammunition might have made it onto the film set.
In April, Rust Film Productions, LLC was fined nearly $137,000 and cited for having a tradition of “plain indifference to worker security” on set, in accordance with a report from New Mexico Atmosphere Division’s Occupational Well being & Security Bureau.


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Kale, they say, is a superfood. But just how super? To Emily Reckard, its powers practically know no bounds. Because in addition to its vitamin-packing, brain-enhancing, waist-slimming abilities, it holds the potential to fundamentally transform schools, neighborhoods, and even the attention spans of some unruly first graders.
As a recent graduate of the nonprofit FoodCorps, which aims to change children’s eating habits by providing more opportunities to eat healthy foods like fruit and vegetables, Reckard has seen firsthand the social benefits of introducing schoolchildren to the world of horticulture. FoodCorps is a culturally and geographically responsive program that promotes a school-wide culture of health by implementing hands-on learning and providing healthy school meals, as well as driving change at a systems level, from school districts to supply chains to state and national politics. In 2017, Reckard was placed at Mundo Verde, one of the 352 schools that FoodCorps works with nationwide. At the bilingual charter elementary in Washington, D.C., Reckard oversaw development of the school’s garden (filled with kale, of course) and implemented a food-focused curriculum that introduced students to gardening, cooking, and healthy eating.

domain: sociology


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Oh that fabric is to die for! Love the colour!
That’s so sweet of you! Thank you!
Thank you! I love it too!
This is a dress made in a very comfortable cotton jersey. The sleeves and the bodice are modified from Ottobre Woman Pattern Magazine. I drafted the skirt myself.
More pictures on my "(Swedish) …
This is all sorts of awesome!
Love the whole outfit but the ikat print skirt is just what I’ve been looking for. Where did you …
Lovely dress and that belt makes the styling perfect!
very cute and great job matching the plaids!
too adorable! :-)
Love the colour and the fit looks great!
Amazing! This has to be one of my favourite versions of this dress.
Wonderful to see something so unusual and different here at Burdastyle once in a while. It looks …
This is my first go at the Truffle dress from Colette Sewing Handbook. I wanted a dress with sleeves so I drafted them myself.
You can see more pictures at my blog…
Adorable kid and such a cute outfit. She has one talented auntie!
I loved this too when I bought the october issue. Your version is very well done!
Sweet dresses, an outfit for a doll, and cute embellishments are fun for the holidays!
Meg's Magazine Mash Up!
Check out Meg's latest mash up of our Straight Skirt, and how she made it look so yummy!
Member Project of the Week
The latest Elie Saab collection inspired this hand-beaded dress by chanel2005.
Celebrate with lovely dresses, sparkly dolman tees, and an embellished peplum skirt.
This week’s new BurdaStyle Videos release is "Sewing a Thread-Chain Belt Loop." Check it out!
You must allow our "request for permission" request to login to Burdastyle with Facebook.

domain: hobby


Virgin Trains emerges as frontrunner to keep West Coast rail line going as franchise debacle is sorted out
Sir Richard Branson’s Virgin Trains could cling on to the West Coast Main Line rail franchise for at least an extra six months under a halfway house plan being considered by the Department for Transport.
Virgin’s franchise expires on December 9 and it was due to be handed over to FirstGroup until the Department admitted major flaws in the bid process.
Uncertainty: Virgin's franchise to run the West Coast Main Line is due to expire
The Government needs to ensure that the service remains operational while a full inquiry into the franchise process is organised and completed. Transport Secretary Patrick McLoughlin will announce tomorrow what will happen to the route.
The service could be taken into State control under Directly Operated Railways, which was set up in 2009 by the Government to run failed rail franchises.
Another option is for Branson’s franchise to be extended for up to two years while the review is conducted, but this could land the Government with legal problems because there are strict competition rules about handing over contracts to a preferred bidder.
Handing the line to Virgin would leave the Government open to legal action from FirstGroup, which was awarded the franchise in August with a £5.5 billion bid.
FirstGroup’s chief executive Tim O’Toole has already said his company is discussing the issue with its lawyers and has not ruled out suing the Department if Virgin is allowed to keep running the operation.
The third option is for a short temporary extension to Virgin’s contract, which could avoid a legal battle or a chaotic handover. Branson has said he would operate the line without a profit for a time, but if he ends up running it for another two years, he is likely to charge.
He has argued that handing the franchise to Directly Operated Railways would kill off Virgin Trains because the firm would not be able to retain its management team.
Two inquiries are to be conducted into the rail system. The first will look at what went wrong with the West Coast bidding process and the second will examine the handling of the whole franchising operation.
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Lasaco, Access Bank, GTBank, Morison, Champions Brew make Ripples Nigeria Stocks-to-watch list
Amid several trade activities last week, few companies in the Nigerian stock market caught the eyes of Ripples Nigeria Stocks Watchlist through their capital market activities.
Some of the companies that made the list are Lasaco Assurance, Access Bank, GTBank, Morison and Champions Brew.
Ripples Nigeria Stocks Watchlist is a selection of stocks monitored for viable trading or investing opportunities. An investor may casually generate a list of equities for investment purposes. But we have taken the pain to do that based on certain parameters in order to save you that hassle.
Kindly note that Ripples Nigeria Stocks Watchlist is not a buy, sell or hold recommendation. It is advisable to consult your financial advisor before making any investment decision.
Lasaco Assurance made Ripples Stock to Watch because the month of March seem to be favouring the insurance firm, as the company’s stock price has been on an upward swing, rising from its bearish state in February to gradually becoming a bull.
On March 1, 2021, Lasaco share price dropped to N1.12, but has since become a hot stock in the capital market, with investors valuing Lasaco share at an higher amount, thereby pushing the stock price to N1.12kobo per share as of March 8, 2021.
Access Bank made the list for the exact opposite of Lasaco. The lender’s stock has been slipping in the capital market, with investors pricing Access Bank’s stock for cheap, while some shareholders were willing to sell at a lesser value.
Access Bank share price fell like a park of cards in the first week of March, losing N0.35kobo in the process. This has affected the bank’s share prixe which fell from N8.15kobo power share to N7.80kobo per share.
As GTBank is preparing to release its earnings report, the lender’s stock price has been fluctuating in the first week of March. While GTBank closed the market on Monday, March 1, 2021, with N13 per share, the stock price rose to N31.70kobo per share on Wednesday.
However, as of Friday, March 5, GTBank’s share price had depreciated to N31.05 per share. The fluctuation in share price shows the low confidence investors have in GTBank, as they price and sell for cheap.
Morison has been one of the best stock in the first quarter of this year. The pharmaceutical marketer had gained N0.11kobo in share price to top best performing stock last week.
Morison gained N0.11kobo to rise to N0.66kobo from N0.55kobo per share. The company is one to watch out for during trading this week.
The alcoholic company topped the chart for decliners following a loss of N0.84kobo from the N2.52kobo share price it opened with, to close with N1.68kobo. Champions Brew is in an industry that has been struggling with government policies and COVID-19.


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The name Hellraid will probably not say anything to most of you. After all, its development was stopped in 2015. However, as the developer Techland announced on April 29 with a teaser, the game will be now still appear: As a DLC for Dying Light.
This is what the DLC offers
The extension begins with a power cut. His investigation leads the survivors into the basement of the tower, where they come across a mysterious arcade machine. It will give you access to a number new content
- Game mode: The DLC will deliver a completely new game mode.
- Dungeon: You fight on a new map
- Opponent: There will be a number of new opponents.
- Weapons: Your arsenal of weapons will be increased.
The developer has not yet released any details on the individual points. However, since the extension based on the set standalone shooter, the new weapons, enemies and the additional area will probably be based on their assets. You can look at them again in the following gallery:
Hellraid – view screenshots
This is how Hellraid was supposed to be
Techland already announced Hellraid in 2012 under the name Project Hell. Originally it was supposed to be a mod for Dead Island, but the developers ultimately decided to add one completely own game to make of it. Your original plans could reveal the content of the DLC.
In the game you should fight alone or with up to three players in a story campaign against the creatures of hell. Each player takes on one of four playable classes. Opponents, items and the like will be there repositioned with each pass
The title was then revised in 2014 and has since been based on the same engine as Dying Light. This was accompanied by a shift to 2015. Before it appeared, however, it was put on hold. Since there was no new information about the project.
Dying Light: Hellraid should appear in summer 2020. The studio has not yet announced an exact release date and price.

domain: game


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PATNA: The Gandhi Maidan police on Friday claimed to have busted a gang involved in duping unemployed youths on the pretext of providing them jobs in a private bank.
One Santeshwar alias Aditya, a resident of Nalanda, had been arrested in this connection, said Gandhi Maidan police station SHO Nisar Ahmad.
Nisar said Santeshwar was arrested from his office in a hotel on Exhibition Road-Station Road where he was taking interview of an unemployed youth, Dharmendra.
He said Santeshwar had asked Dharmendra to give him Rs 30,000 if he got the job in the bank. Dharmendra later lodged an FIR against Santeshwar with the police station concerned, the SHO said.
Describing the modus operandi of Santeshwar, the SHO said advertisements regarding jobs were published to lure unemployed youths and they were asked to pay the money after getting the jobs.
The police are interrogating the arrested youth to elicit more information about his associates, the SHO said, adding the private bank concerned has denied any connection with Santeshwar.
Jumps from flyover: A married woman made an abortive bid to commit suicide by jumping from the Chiraiyatand flyover on Friday afternoon.
As she was in a sub-conscious state, her identity could not be ascertained immediately, Gandhi Maidan police station SHO said and added she has been admitted to PMCH.
Meanwhile, police sources said the woman, wife of a telecom employee, is a resident of Biharsharif.


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As an organization of people with passion for helping the world, we donate our Salesforce Consulting skills to help organizations who are improving the lives of everyone in our communities. Safe Schools for Alex, led by Alex’s father, Max Schachter, is an organization that has formed a nationwide school safety commission with the goal of creating standards and actionable school safety recommendations to prevent future tragedies like the one where Max lost his son.
If you would like to support Safe Schools for Alex or learn more about what you can do to help this important effort, please go to their website or contact them. https://safeschoolsforalex.org/
Thank you to Salesforce for promoting ProBono work this week and encouraging others to give back. Once you start, you can't stop, the joy of helping others is too great.


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Features two advanced techniques:
Color Scoop & Petal Spray. Both techniques allow for customization so that you can really product something that is individualized for each client. Color Scoop is a great option for a partial highlight. This technique results in beautiful sweeps of color throughout the top of the head and around the face. Petal Spray is perfect for hair that needs added dimension. The mixture of strong and soft color design creates petals of brightness that shift throughout the hair. Adrienne also shares her experiences and discusses several topics such as the importance color plays in our everyday lives and professionalism.
Running Time: Approximately 65 minutes.
About the Educator:
Adrienne Rogers has developed a broad range of experience during her 17 years in the beauty industry. She is a senior colorist at Cutler Salon SOHO in New York City. The Cutler salon team designs color for hundreds of models and Adrienne’s work has been featured in various Glamour Magazine makeovers.

domain: artistic


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It goes without saying that T.I. is one of the most recognizable, and commercially successful hip hop artists in the history of the genre. With 7 billboard charting studio albums already under his belt, 7 of which went platinum at least once, and three which peaked at #1 on the US charts, it would be hard to deny the impact that he’s had on bringing Southern hip hop onto the national stage. While guys like Andre 3000, Big Boi, Trick Daddy, and Master P may have originally started drawing ears to the hip hop coming from the bottom half of the map, it’s guys like T.I., Lil’ Wayne, and Ludacris that brought it to the “mainstream” of pop culture.
Even more impressive than his sales success, has been his ability to maintain an unquestionable level of respect in the grittier side of the industry, while still propelling his career with pop leaning hits like “Whatever You Like” and “Live Your Life”, combined with the fact that he’s been the star of a few reality shows. But, as strange as that dichotomy sounds, it’s probably to be expected from a guy with a ridiculously long rap sheet, who at the same time is known as one of the most charitable philanthropists in the game. With his newly released 8th studio project, Trouble Man: Heavy is the Head, T.I.P. is trying to add another release to his strong legacy of crossover success.
To cut straight to the chase, it’s hard to say that this album is anywhere near his strongest, from either a commercial or artistic standpoint. It’s definitely an enjoyable experience, with a few definite highlights. But, it does feel like it lacks that certain raw, almost cinematic, energy that tracks like “What You Know” or “Rubber Band Man” had, and at the same time, doesn’t really have any sure fire pop hits like the songs we mentioned just a second ago. Granted, those singles were responsible for making T.I. the superstar he is, and would be tough for anyone to follow. But, if that’s what you were looking for here, you might be a little disappointed.
From a lyrical standpoint, and a bit unexpectedly, T.I. goes in much more aggressively and faster than we’ve seen from him in a minute on the Trouble Man album. On tracks like the standout, but deceptively boringly titled, “The Introduction”, “G Season (feat. Meek Mill)”, “Go Get It”, and “The Way We Ride”, along with a few others, we get a set of almost throwback performances that definitely have hints of the type of hunger you wouldn’t expect from a vet like T.I.P. He’s definitely not reinventing himself at all here, with fast paced but simple flows and clever but relatively straightforward wordplay. It definitely makes for some strong, yet not so varied rhymes.
But, that lack of variety definitely holds this one back a bit, to the point where you could probably flip flop T.I.’s verses from one track to the next, and not even notice – at least when he’s taking the high energy approach, which he does on 75% of the tracks. By no means does it ever cause the album to get “boring”, but it can drag on a bit.
There are a few tracks, which are mostly feature driven, that do take a different approach, but they’re largely hit or miss – though mostly “hit”. The Andre 300 featuring “Sorry” is probably the most complex song on the album (which probably isn’t a surprise). We also get some other interesting collabs like the album’s strongest standalone single “Hello (feat. Ceelo), “Widlside” which showcases a more aggressive than usual A$AP Rocky, and “Ball”, which includes a shockingly not lazy verse from Lil’ Wayne.
However, there are a few gigantic misses from a songwriting or lyrical standpoint. T.I.P.’s obvious attempt to gain some crossover traction “Guns and Roses” with Pink, while a decent representation of a bittersweet relationship, just isn’t executed as well as it could have been. “Cruisin” seems to be a spiritual successor to “Whatever You Like”, but falls flat, which combined with its singsong approach, is pretty cringe worthy. While it’s tough criticize his intentions with the religiously themed “Hallelujah” the bible school favorite “Jesus Loves Me” driving the hook feels is a bit misguided.
From a production perspective, the experience is almost exactly the same story as the lyrical approach. A majority of the album is driven by an almost throwback T.I. feel, but it just lacks a certain appeal and energy that some of his classic records carried. There’s a liberal use of snares, which gets filled out with some heavy, rolling bass. However, when that’s the case, it’s tough to differentiate one track from the next. These tracks all boast some quality and polished beats, which sees T.I.’s longtime producer DJ Troop shine on “The Introduction”, and the Hit Boy provided “The Way We Ride” definitely knock the hardest.
When this one really shines from a production standpoint is when it takes a few chances. The more outside the box productions, at least as T.I.’s work would be concerned, might make for the most interesting tracks from a musical perspective. The Jazze Pha provided “Sorry” carries a much darker tone than most of the album – to excellent results – and relative unknown DJ Montay provides a smooth as glass beat on “Can You Learn (feat. R. Kelly). However, and maybe not so surprisingly, the strongest instrumental here is without a doubt the Pharrell production, “Hello”.
Overall, Trouble Man: Heavy is the Head, is a solid effort, and somewhat predictable, album from one of the South’s true legends. It isn’t anywhere as strong as Urban Legend or King, and probably fits somewhere in the middle of his discography as far as overall quality is concerned. Unfortunately for T.I., he has a ton of competition right now for record sales, and it’s probably go to show in how much attention and commercial success this one gets. However, it’d definitely be a solid purchase for any fan of T.I., accessible Southern hip hop, or upbeat rap in general.


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TAYLOR, Mich. -- Charges have been dropped against two Black teenagers accused of killing a White mother of six.
Police say ballistics tests show neither had a hand in shooting Wendy Meinke, who was killed July 30 during a dispute involving her son and others at an apartment complex in the Detroit suburb of Taylor. She tried to intervene, and two people shot in her direction.
Taylor police later stopped a car carrying Devin Plummer, 17, and Franklin Smith, 18. Plummer was charged with first-degree murder and Smith with being an accessory for driving the car.
The charges were dismissed after prosecutors received gun-residue tests Wednesday indicating it was highly improbable Plummer could have fired the gun police think is the murder weapon.
The sudden reversal came two days after relatives and Black activists protested outside 23rd District Court. They accused the Taylor police department, which has no Black officers, of racial profiling. The teens have no criminal records, The Detroit News reported.
A 14-year-old from Taylor, who also had been held in the shooting death, has since been released, Jack Fennessey, a spokesman for the Wayne County prosecutor's office, told the newspaper.
A decision was made not to charge him, Fennessey said.

domain: news


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Do you know someone who adores cute cats? Here's some great gift wrap for cat lovers (or their cats!) that makes the perfect complement to their present. Click the picture to see bigger and to personalize...
tagged with: redroadstudio hand-drawn illustration, cat cats kitten kittens cute, dancing animals fun girly, kitsch pink multcolor multicolour, trendy trend for her mom, girlfriend bff aunt partner, kitchen electronic accessory home, paw print feline cat lover, birthday gift christmas present fun
Lovely pussy cats dancing over their paw prints! What a lovely kitsch accessory for all you cat lovers out there! A perfect gift at any time for your feline friends!
»visit the redroadstudio store for more designs and products like this
The Zazzle Promise: We promise 100% satisfaction. If you don't absolutely love it, we'll take it back!

domain: pet


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Push Production Capacity, Sido Muncul Will Inaugurate New Factory
By : Hariyanto And Aldi Firhand. A | Thursday, January 04 2018 - 21:00 IWST
PT Industri Jamu dan Farmasi Sido Muncul Tbk (SIDO)
INDUSTRY.co.id - Jakarta - Manufacturers of traditional herbal medicine, PT Industri Jamu dan Farmasi Sido Muncul Tbk (SIDO) will increase its production capacity by expanding the current plant utility to two-fold.
"The plan will begin to be realized in April 2018. Later on will be inaugurated a new factory that produces products especially in liquid form," said President Director of PT Industri Jamu dan Farmasi Sido Muncul Tbk, Irwan Hidayat to the press in Jakarta, Thursday (4/1/2018 ).
He revealed, the factory will be utilizing a highly automated technology that can increase production greater than today. "The location of the new plant remains in Semarang," said Irwan.
By the end of 2016, SIDO has a production capacity of about 80 million sachets per month with utility rate reaching 85%.
Meanwhile, to boost its export performance, Sido Muncul plans to open a marketing office in the Philippines in March this year.

domain: economics


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Inside: Forget Me Not Flower Tattoos Ideas & Designs.
The forget-me-not flower symbolizes remembrance, reflection, memory, mourning, and respect. Whether you’re looking to memorialize a loved one or simply admire the beauty of nature, forget-me-not tattoos are one of the gorgeous choices available. These flowers are often bright blue but can be found in other colors as well. Take a look at these fantastic forget me not flower tattoos to get inspired!
Forget Me Not Flower Tattoos
Blooming Tattoo Designs
These pretty Forget Me Not Flower Tattoos are one of a kind.
Pretty Forget Me Not Flower Tattoos
15. Tiny Blue Flower
19. Dainty Bundle
More Stunning Design Ideas
- Unique Leaf Tattoos – Just like the leaves themselves, the options and meanings behind a leaf tattoo are practically endless. They are a fantastic choice for anyone wanting to represent the life cycle and the way we all change as time goes on. Rebirth, death, and life are all symbolized in leaf tattoos. You’ll find small and dainty designs as well as large leaves that stand out. There are several color ideas as well. Be sure to check out these popular leaf tattoo designs!
- Daisy Tattoo Ideas – Daisy tattoos are some of the most beloved designs in the tattoo industry, and you don’t have to look far to see why! With their bold simplicity and delicate elegance, daisies will always be in style. Whether you’re looking for something bold or sweet, one of these timeless daisy tattoo designs are sure to catch your eye.
- Flower Tattoo Ideas – It’s no surprise that flower tattoos can be insanely beautiful, unique, and meaningful. Depending on the size and shape of the tattoo design, they can pretty much go anywhere on your body and look fantastic. While many flower tattoos are elegant and feminine, there are some pretty popular ones that are more masculine. A large black rose tattoo is hard to beat because they look so cool. But big, brightly colored blossoms are also incredible too. No matter what your style is, I’m sure you’ll find a flower tattoo that is calling your name.


A December 6th article from The Engineer discussed a new advancement in ultrasound diagnostic technology. Researchers at Nanyang Technological University in Singapore have developed a 3D printed lens that generates enhanced ultrasound or photoacoustic waves compared to current machines. The lens is made from resin, which allows the waves to focus sharper than current glass options and leads to better and more precise images.
Ultrasound beams are created when high-frequency sound waves are fired at a lens. While standard ultrasounds are cylindrical or spherical, 3D printed resin lenses can be created in much more complex shapes allowing for the ultrasound to be focused onto multiple sites or shaped to direct energy a certain way. The lenses can also increase the therapeutic use of ultrasounds such as breaking up blood clots, destroying tumor cells, or controlling the release of drugs from an implant.


About Our Product
This knock-down rack can be used to complete multiple tasks in your commercial bakery, making it easy to be more productive.
- This knock-down pan rack has 15 levels.
- Our racks are built with all-welded constructions, which includes the side panels.
- Full pan space clearance between middle cross-section support tubes and lower pan slide.
- Simple assembly – only one size, heavy duty fastener set.
- Sturdy zinc-plated steel stem casters with 5” x 1 ¼” soft rubber wheels.
Use this rack to cool baked goods after taking them out of the oven, use it to transport your baked good, or use it for storage! This is a great rack to use in the catering business, supermarkets, or restaurants since you can break it down when not in use to save space. This durable pan rack canhelp in a variety of industries and is a great option for your commercial bakery.
- 4" spacing between levels
- Each rack holds one 18” x 26” sheet pan per level
- Overall dimensions: 20 ½” W x 25”L x 69”H
- 1” square x 1/8” wall tubular structure
- 1” x 1 ½” x 1/8” aluminum angle for pan slide material
When you need a pan rack that can be used in your bakery in a variety of ways, this knock-down rack is for you! Shop Schaumburg Specialties now.


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SPOILER WARNING, the following article contains major spoilers for Injustice 2 #44 by Tom Taylor, Bruno Redondo, Mike S. Miller, Juan Albarran, Rex Lokus, J. Nanjan and Wes Abbott, on sale now.
While the Injustice games may be known for being ultraviolent slugfests between all of your favorite DC heroes and villains — as well as some guest stars too — the Injustice comics are actually some of the most thoughtful, well-crafted, heroic comics around right now. They still have ultraviolent slugfests between all your favorite DC heroes and villains, but the creators pretty much have free reigns to color outside of the lines established by the hit fighting franchise and focus on what characters not featured in the games are up to in a world where Superman rose to power as a dictator and was brought down by a Batman-led insurgency.
Injustice 2 the comic is able to give a spotlight to the likes of Booster Gold, Plastic Man and the Teen Titans, filling in the gap between the two games and jumping between different stories and protagonists in a way that feels comparable to the now-classic weekly series, 52.
Recent issues of the weekly digital installments have shown the best the comic has to offer and while Kal-El may be locked away for his crimes, a new Superman has stepped up to lead the heroes of the DC Universe and remind its people what that crest truly stands for.
One of the biggest omissions of the Injustice games is the Teen Titans roster; outside of Cyborg and Raven, they just aren’t present. This was actually acknowledged in an Annual for tie-in comics to the first game, Injustice: Gods Among Us. Several of the Teen Titans were present in Metropolis when The Joker’s nuclear bomb exploded, wiping out the city. Kid Flash was killed in an instant and while Superboy tried to save Beast Boy, he wasn’t able to shield his friend from the impact of the blast. Faced with the news that The Joker had forced Superman to kill Lois Lane and that Superman has killed The Joker in response, Superboy held firm to the ideals Kal-El taught him even when Superman himself abandoned them, bringing him into conflict with his mentor.
When it became apparent that Superman couldn’t be stopped, Superboy attempted to trap him within the Phantom Zone. But even though he was backed by Red Robin, Starfire and Wonder Girl, it wasn’t enough to defeat the Man of Steel. A super-strong punch fractured Superboy’s ribs, which then pierced his heart, causing the Boy of Steel to bleed out internally. In order to save his life, Superman trapped him and the other surviving Teen Titans in the Phantom Zone, where they resided throughout the events of the first game -- until it came time for a Kryptonian breakout led by Batman and his crew.

domain: entertainment


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At InterDigital IP and Innovation Conference, European and Industry Experts say Maintenance of IP Protections is Crucial
Intellectual property protection is one of the most crucial components to strengthen the technological sovereignty and competitive advantage of France, and the European Union. At a time when emerging technologies like AI, 6G, virtual reality, and augmented reality are becoming essential for many sectors of the economy, it is important to know how to protect intellectual property and strengthen cutting-edge research in this dynamic and changing ecosystem.
France and the EU must strengthen their strategic autonomy and technological sovereignty: this is one of the obvious lessons of the consequences of the COVID crisis and the unstable geopolitical context. To enable the development of world-class European champions, companies must invest in the development of their intellectual property, but also in their ability to protect and enhance it. Intellectual property must be a central element of any innovation strategy and an essential part of French and European recovery plans.
For this purpose, patents and licenses are incentives for investments in research and development of standards.
This issue was the focus of a roundtable discussion organized by InterDigital on September 30 in Paris, in conjunction with the rollout of the France 2030 Plan and the European Union's Innovation Agenda, in partnership with France Brevets and entitled "Innovation and Intellectual Property: What Responses from France and Europe."
The event brought together several high-level experts to discuss these issues, including Innovation Policy Advisor and Access to Finance at the European Commission and Director General of Research and Innovation Marie-Josée Rodi-Andrieu, France Brevets Director General Didier Patry, AFNOR’s Director of Innovation and Standards Franck Lebeugle, Orange Group’s Director of Research Jean Bolot, and Director of R&I - R&D at InterDigital Valérie Allié.
Throughout their discussions, the speakers reviewed the most effective tools to support innovation and research, promote the strategic impact of intellectual property, and discussed methods to better protect standardized technologies.
Here are the main points discussed and the experts' analyses on them:
KEY TAKEAWAY: For innovative companies, there is a positive correlation between owning intellectual property rights and the economic performance of companies. SMEs that own IP rights have 68% more revenue (1).
- European Commission’s Marie-Josée Rodi-Andrieu shared: "Intellectual property is a source of revenue for companies and therefore for the economy of the European Union as a whole. A reliable intellectual property framework is the best way to harness creativity and enable innovative companies to grow. IP is also a key lever to support the EU's resilience and economic recovery in times of crisis. [...] This is why further progress in this area is mentioned in the new European Innovation Agenda."
KEY TAKEAWAY: Start-ups, SMEs and French and European companies need incentives to invest in research and innovation, support the development of core technologies and work towards their integration into global standards. Without these incentives, companies would lose their leadership in global standards bodies, which could also jeopardize the interoperability of technologies. One potential solution would be to make better use of existing European Commission legislation to fight countries or companies guilty of intellectual property theft or "hold out" (the practice of using patented and standardized technologies without paying the required license for their use).
According to Orange Group’s Jean Bolot, “Intellectual property is vital for large companies. The acceptability rate of patents filed, and the acceptance rate of publications are markers of excellence awarded to the company.”
KEY TAKEAWAY: Europe needs to put in place more incentives to invest in research, which is essential to achieve the EU's strategic goals, including support for the digital and green transition. The continued development of wireless technologies like 4G/5G/6G and WIFI is critical to the success of the EU's industrial policies on autonomous cars, connected machines and IoT devices, chip manufacturing and cloud computing, etc.
Didier Patry, Managing Director of France Brevets, explained: "If cutting-edge and disruptive technologies developed by innovative companies including start-ups and SMEs are used by other players without proper authorization and without a license from the company which invested in such R&D, this creates a competitive disadvantage, removes all incentives to invest in R&D and take risks, and consequently directly threatens the entire innovation and deep-tech ecosystem at a time where France and the EU invest massively in R&D-intensive and IP-rich programs. We must therefore fight vigorously against those who compete unfairly and take advantage without providing any return to the innovators who contribute to a better society."
InterDigital’s R&I Director Valérie Allié added: "The European Commission needs to recognize and emphasize the link between its overall actions and objectives and the potential negative impacts of the evolving framework for standard essential patents (SEPs). A disconnect between these two elements could reduce the return on R&D investments and discourage innovators from bringing new technological solutions to standardization processes. In general, we advocate market-driven solutions and enforcement of existing rules rather than the introduction of top-down legislation and new obligations, especially for research-intensive companies."
KEY TAKEAWAY: Successful digital transition requires systemic coordination in the development of and adherence to policies for standard essential patents. The European Union has a proven track record of defending its innovative companies in the 5G ecosystem. Today, the EU must maintain consistency and an in-depth understanding of global issues, including coordination of IP policies to support a level playing field with third countries.
Franck Lebeugle, AFNOR’S Director of Standardization Activities added: "The European standardization organizations are formidable tools for promoting intellectual property and bringing innovation to our companies at the European level and beyond. As European Commissioner Thierry Breton reminded us when the new European standardization strategy was published, it is not so much a question of changing the rules that currently prevail, but to make full use of them for the benefit of our companies and, more broadly, our industrial sovereignty."

domain: law


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Monica Banks has been creating public works, site-specific installations, and showing work in museums, galleries, and other venues for 30 years. She has won awards for recent work from Jocelyn Miller, assistant curator at MOMA PS1; Benjamin Genocchio, former art critic for The New York Times; Marla Prather while curator in the Department of Modern and Contemporary Art at the Metropolitan Museum of Art NYC; and the curators at the Heckscher Museum of Art. In 2016 Jorge Pardo selected her work to be shown alongside his own work at the Parrish Art Museum “Artists Choose Artists.” Her work has recently been featured in The New York Times, Brooklyn Rail, Smithsonian Magazine, Timeout New York, The Baltimore Sun, East Hampton Star, Sag Harbor Express, and Hamptons Art Hub, and she has had solo or dual shows at Sara Nightingale Gallery in Sag Harbor every year since 2015.
She created “Faces: Times Square,” a block-long sculpture which stood in Times Square from 1996-2009, for which she won an award from The Public Design Commission of the City of New York. Her permanent public works are located in the Bronx, Binghamton NY, Charlotte NC, and West Nyack NY. She has done site-specific installations at The Carriage House at the Islip Art Museum, The Rockland County Center for the Arts, The American Craft Museum, Spring Break Art Show, and other venues. Permanent collections holding her work include the Parrish Art Museum, The University Museum of Contemporary Art at University of Mass. Amherst, the Islip Art Museum, and the Daura Gallery at Lynchburg College.


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Author: Ian Daglish
Operation Goodwood: Over The Battlefield
Pen and Sword Military
Size: 12 Mb
For the first book in our new series Over The Battlefield, we have chosen Ian Daglish to describe the events of Operation GOODWOOD, July 1944, the dramatic attempted British armoured break-out from the Normandy bridge-head. This was the greatest armoured battle undertaken by the British during the Second World War. What is so special about this book is the discovery and use of superb aerial photos taken during the fighting by the RAF. This amazing imagery makes it possible to trace the course of the battle and to track the movement of the armoured regiments and troops of both sides. The effect is sensational and the reader is able to follow history in the making.

domain: history


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There are many different urban centers in the world one enjoy and you can accept brand new lesbian, gay, bisexual and you may transgender (LGBT) people. These locations features in fact improved and you may mature financially by attracting perhaps one of the most very important and you will powerful public categories of the newest twenty-first 100 years. Organizations off rooms, eating, taverns, bars, clothing places and you will health spas that appeal to a unique section that is choosing the best of the best. We took lots of facts under consideration in order to get this to post, including courtroom defense, social acceptance, Gay and lesbian nightlife and you will monetary potential and you will created the set of 11 Really Homosexual-Friendly Metropolises In the world.
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Madrid comes with the premier gay community inside the Spain and something off the greatest within the European countries, having an estimated five hundred,100 Gay and lesbian anybody living in the metropolis. Chueca ‘s the cardiovascular system of the Lgbt society in which discover the highest concentration of gay pubs and you may golf clubs. Chueca initiate in the Gran Through and operates northern to possess 1km and you may it stretches east in order to west ranging from Calle Hortaleza and Paseo de- Recoletos. Contained in this city, homosexual wedding is actually greeting plus the gay area have swayed mainly the latest city’s trendiest accommodations, dinner and you can stores. The July Madrid computers the most significant homosexual pride into the European countries. Madrid’s gay satisfaction, titled Orgullo, draws a crowd from nearly dos billion people.
Miami, United states of america
Miami has long been called a well-known and unlock attraction to own Gay and lesbian travelers seeking to relax, manage its tans, fulfill anybody and you will dine from the chicest eating. The fresh new Gay and lesbian neighborhood has actually constantly noticed invited when you look at the Miami. All year there are shores popular with the new homosexual audience, nightclubs and sites, functions and much more, however it is the new annual Miami Coastline Gay Pride Event you to provides anyone with her in a colourful occasion and parade.

domain: geography


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Hyaluronan-linked protein 1 (HAPLN1) which has been shown to be highly expressed in malignant pleural mesotheliomas (MPM), was detected in serum using an electrochemical surface-imprinting method. First, the detection method was optimized using Bovine serum albumin (BSA) as a model protein to mimic the optimal conditions required to imprint the similar molecular weight protein HAPLN1. BSA was imprinted on the gold electrode with hydroxyl terminated alkane thiols, which formed a self-assembled monolayer (SAM) around BSA. The analyte (BSA) was then washed away and its imprint (empty cavity with shape-memory) was used for detection of BSA in a solution, using electrochemical open-circuit potential method, namely potentiometry. Factors considered to optimize the conditions include incubation time, protein concentration, limit of detection and size of electrode. Matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was used to confirm selectivity of imprints. With the obtained imprinting control parameters, HAPLN1 was imprinted in duplicate and the detection of spiked HAPLN1 was successfully conducted in serum.
Citation: Mathur A, Blais S, Goparaju CMV, Neubert T, Pass H, Levon K (2013) Development of a Biosensor for Detection of Pleural Mesothelioma Cancer Biomarker Using Surface Imprinting. PLoS ONE 8(3): e57681. https://doi.org/10.1371/journal.pone.0057681
Editor: Mohammad O. Hoque, Johns Hopkins University, United States of America
Received: May 10, 2012; Accepted: January 28, 2013; Published: March 13, 2013
Copyright: © 2013 Mathur et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This study has been funded by an army grant (W911NF-09-1-0499) for Potentiomeric detection of pathogens. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
A possible cure of cancer would be more likely if the disease could be detected early for optimizing the therapeutic procedures. Extra-cellular matrix (ECM) has its importance not only for the physical support of various cells but also in cell-cell interactions, cell signaling and cell repair processes. Therefore ECM proteins themselves present a potential monitoring target reflecting the ongoing changes. If the changes occur due to the presence of a disease such as cancer, the detection of these changes would be a presumptive disclosure of cancer before any symptoms appear. As an example, heparanase enzyme , an endo-ß-glucoronidase, is a multifunctional enzyme influencing key steps of development of numerous types of cancers such as gastric , non-small lung , esophageal , head and neck , breast  and pancreatic  cancers as examples.
Heparanase cleaves enzymatically heparin sulfate and with serine proteinases and metalloproteinases plays a crucial role in the extracellular matrix remodeling associated both with in situ tumors growth and the metastatis-related invasion of cancer cells. The enzymatically inactive form of heparanase has been shown to affect cell signaling, and thus increasing cancer growth with upregulation of several genes expression . Commercial biomarkers for monitoring asbestos-induced mesothelioma activity are mesothelin and soluble mesothelin related peptide (SMRP). Osteopontin (OPN) and CA125 have similarly been related to cancer-linked heparanase activity in addition to hyaluronan, TRA and ferritin. The ECM remodelling events may certainly be a target for diagnosis of the early stages of cancer invasion  as well as targets for anti-cancerous treatments. Hyaluronic acid (hyaluronan, hyaluronate, HA) is a linear, very high molecular weight glycosaminoglycan, is one of the most abundant ECM proteins. HA has been shown to exist at high levels in colorectal, epithelial ovarian, and breast cancers – and also tumor growth inhibition has been evidenced when HA binding motifs have been affected . HA overexpression is greatly dependent on the CD44, so the regulation of bladder cancer growth, invasion and angiogenesis was proven to occur through the CD44 by HA synthase-1 expression . As the HA expression seems to be clearly elevated at the appearance of cancer it is not surprising that Ivanova et al have shown that also HAPLN1 protein, which helps to link proteoglycans to a HA core, has been shown to be elevated in cancer cells.
Malignant pleural mesothelioma is a rare malignancy caused by asbestos exposure, which has a poor prognosis. Cartilage link protein HAPLN1 is being studied as a candidate biomarker, found to be overexpressed in mesothelioma stage 1 along with a highly expressed cancer biomarker osteopontin (OPN1) located in the same gene set . Immunoassays are commonly used for detection of cancer biomarkers as they show superior selectivity, but can be time consuming and expensive. The biological features of markers such as heparanase emphasize the importance to design cost effective methods allowing the early detection of such biomarkers in serum and tissues both for diagnostic and prognostic purposes as well as for monitoring cancer treatments.
Also detection of very low concentration of such biomarkers with ELISA tests is difficult. Therefore, there is an urgent need for development of new technologies, which are rapid and very sensitive to detect low concentrations of biomarkers. Early diagnosis will provide more time for treatment of cancer, which is crucial to save a life. Molecular imprinted polymers are being considered as a suitable replacement for antibody-based systems as biosensors, due to better stability and reusability of the bio-recognition surfaces . Biosensors are composed of two components, bio-receptor element and signal transduction component. A bio-receptor surface made with molecular imprinted polymers would be two orders of magnitude cheaper then antibodies. As price of the hydroxylated alkane thiol polymer would cost about $0.2–0.6 mg−1 compared to antibodies, which vary depending on the target. Antibodies generated for targeting human HAPLN1 protein would cost about $900–$1000 mg−1. Even low density of such antibodies on immunoaffinity cartridges have higher price than molecular imprinted polymers, costing between $10 to $100 mg−1. High price of HAPLN1 antibodies increase the cost of HAPLN1 Elisa kits commercially available to $10/test. Whereas an imprinted gold coated sensor chip (1 cm×1 cm) would cost approximately $ 1.2/test and can be used repeatedly for multiple assays unlike antibodies, which cannot be reused.
In our study we aim to develop a tool for early detection of biomarkers. Target protein molecules co-adsorb on the surface of gold electrode with the thiols and they together form a well-packed monolayer. Removal of the protein molecules from the SAM form imprint cavities and the functional hydroxyl head group of the thiols provide specificity to cavity. The complementarity in size, shape and hydrophobicity provides affinity towards the same molecule, which has been imprinted.
The imprinted electrode is then used to assay the template in buffer using potentiometry. As the charged protein molecule in buffer binds to the cavities it changes the potential of the sensing electrode. Synthesis of the imprint surface is straightforward and does not require any expensive technology for preparation. It is an old but promising technology, which has been developing with the challenges it has faced in the past.
In 1930 a Soviet scientist M.V Polyakov demonstrated that silica gel prepared in the presence of a solvent additive showed preferred binding to the same solvent . Linus Pauling and Frank Dickey published in 1949 that silica shows specificity for dyes imprinted on the surface . Major breakthrough in molecular imprinting took place in 1972 when Gunter Wulff prepared molecular imprinted organic polymers using the covalent binding approach, which can differentiate between enantiomers of glyceric acid . Wulff et al. used an approach, which became the most famous for molecular imprinting studies during the 70's until another theory was introduced in 1981 by Mosbach and coworkers. They created molecular imprints based on non-covalent binding with the template . It was the simplicity of this approach that triggered an explosion in 90's with molecular imprinting polymers. We have used non-covalent binding approach introduced by Wulff et al. to imprint the HAPLN1 biomarker by using hydroxyl functional groups on thiols which form weak interactions with the protein and a well fitted mold around the same. This provides more specificity to the cavity, thus making the removal of HAPLN1 from the cavity easier. The imprint conditions were optimized for potentiometry using BSA (66 kDa) protein (Figure 1), due to its similarity in size with HAPLN1 cancer biomarker (65 kDa). We tested the specificity of the imprinted cavities, using MALDI-TOF, by performing tests to show that a relatively smaller protein Myoglobin (17 kDa) does not bind to BSA imprints.
Materials and Methods
Principle of potentiometric detection
In our technology, we have used potentiometry for detection of target analyte HAPLN1. A potentiometer measures the potential difference between a reference electrode (Ag/AgCl) and the working electrode. Analyte binds to the bioreceptor surface on the working electrode in a buffer solution, resulting in a change in potential difference between the two electrodes. This change is then measured by the system. Smaller biomacromolecular ions do not result in a voltage as they remain in equilibrium with the electrolyte.
The strong voltage response results from the macroions binding via extensive macromolecular hydrogen bonding around the imprint On the working electrode (Gold coated silicon chip), a large biomacromolecule recombinant HAPLN1, 65 kDa size protein assembles with thiols, which form an organized, dense SAM around the analyte. Thiols have the tendency to self assemble on the gold surface as the sulfur group on the tail end of thiols forms a sulfur-metal bond with gold . Organized SAM is an important ‘sealant’ against interferrents and pinholes for parasitic current. The macromolecule is then washed from the surface, which results in formation of cavities. The imprinted electrode acts as the bioreceptor element of the biosensor. Thus imprinted electrode works with same principles as commercial ion selective electrodes, which have a specific ionophore to recognize the analyte ion. We show that potentiometry can be used with good sensitivity and selectivity to detect the target cancer biomarker in buffer solution and in spiked serum. The electrochemical response obtained with this technique is due to the potential change after the binding of the template protein. The change in potential is measured with respect to a reference Ag/AgCl electrode , .
Materials required for electrochemical measurement
Bovine Serum Albumin (BSA) from bovine blood (Mw = 66.3 K), Myoglobin from equine skeletal muscle (Mw = 17.6 K), 11-mercapto-1-undecanol (97%) (thiol), absolute ethanol, methanol, and isopropanol were purchased from Sigma Aldrich (St. Louis, MO, USA). Human HAPLN1 (64.68 kDa) full-length ORF (AAH57808, 1–354 a.a.) recombinant protein with GST-tag at N-terminal was purchased from Abnova Corporation (Walnut, CA) and stored at −80°C. All the proteins and chemicals were used as received. Two kinds of electrodes were used as the sensing electrode: the gold (50 nm) sputter coated silicon wafer (1 cm×2 cm) and gold electrodes (2 mm2) purchased from BASi (West Lafayette, IN). The potentiometric measurements were made in 10 ml 1X PBS in 20 ml glass vial, equipped with a magnetic stirrer. When diluted to a 1X concentration, 10 X phosphate buffered saline solution purchased from Sigma Aldrich (St Louis, MO) yield a phosphate buffer concentration of 0.01 M and a sodium chloride concentration of 0.154 M with pH 7.4. The two-electrode system consisted of an Ag/AgCl as the reference electrode and silicon coated gold chip gold electrode as the working electrode. The potentials of the working electrode against the reference electrode were measured with an Accumet AR15 potentiometer purchased from Fisher Scientific (Pittsburgh, PA) and EMF 16 channel Electrochemical Interface purchased from Lawson Labs (Malverin, PA)
Materials required for MALDI TOF
Gold target plate was purchased from Bruker Daltonics (Billerica, MA). TA solution (0.1% trifluoracetic acid/acetonitrile, 2∶1,vol∶vol) and Sinapinic acid were purchased from Sigma-Aldrich (St. Louis, MO) for the matrix preparation to immobilize the protein bound on the surface of the target plate. Bruker Autoflex MALDI-TOF purchased from Bruker Daltonics (Billerica, MA) was used to determine the specificity of protein binding to respective imprints on the surface of the target plate. Chemical resistant conformable PTFE (Teflon) Tape was purchased from CS Hyde company (Lake Villa, IL). Nitrogen gas was supplied by Presto-O-Sales and Services (LIC, NY).
BSA Sensor Fabrication
The silicon chips were cleaned by sonication in de-ionized water, methanol and isopropanol. Silicon chips were allowed to dry overnight and then sputter coated with gold. The gold coated chips were then washed with de-ionized water and dried with pure nitrogen gas. Similarly gold electrodes were cleaned with 0.1 and 0.05 micron alumina and then sonicated with de-ionized water to remove any bound alumina particles. Since proteins denature in non-aqueous solution and thiols show poor solubility in aqueous solution proteins were dissolved in de-ionized water whereas thiols were dissolved in absolute ethanol. Compromising mixed solutions of 19∶1 and 18∶2 (water∶ ethanol) with final thiol concentration of 0.1 mM and 0.2 mM were prepared in clean glass containers and comparatively studied to achieve good solubility of thiols and avoid protein precipitation. The gold coated electrode is then dipped inside the mixed solution to form the self assembled monolayer of protein and thiol. The head space of the container is filled with inert nitrogen gas and covered with parafilm. Incubation time of the chip in the mixed solution was varied for a better imprinted surface. The gold chip is then thoroughly rinsed with de-ionized water to remove the bound protein. Also different protein concentrations of 30 µg/ml, 10 ng/ml and 1 ng/ml were used to form the imprints and the binding results were compared. The electrodes prepared were then used to check binding response with variable BSA concentrations in buffer solution.
Preparation of Imprints on MALDI-TOF target plate
Except the surface containing 384 wells, the rest of the gold target plate (8 cmx12.5 cm) was covered with nonreactive Teflon adhesive tape. The gold target surface was cleaned with ethanol and de-ionized water. Controlled flow of inert nitrogen gas was used to dry the target surface. Solution A was prepared with a 19∶1 mixture of 16.17 mg Myoglobin (i.e 1.7 µM) in 540 ml de-ionized water and 0.22 g 11-mercapto-1-undecanol (i.e 2 mM) in 540 ml absolute ethanol. This solution was formed to create myoglobin imprints on one half of gold target plate. Solution B contained 19∶1 ratio mixture (water∶ ethanol) of 16.17 g bovine serum albumin (i.e 0.432 µM) dissolved in 540 ml de-ionized water and 0.22 g 11 mercapto1-undecanol (i.e 2 mM) in 540 ml absolute ethanol. This solution was prepared to create BSA imprints on the other half of gold target plate. Half of the Au plate was immersed in Solution A for 12 hrs to co-absorb the protein and thiol, whereafter it was dried with pure nitrogen gas. Similarly the other half of the gold target plate is immersed in solution B for 12 hrs and dried with pure nitrogen gas. Protein and thiol concentrations were kept same as used for imprinting on gold electrode for potentiometry. Cavities that are complementary with the template proteins were created in the monolayer by removing the protein molecules through repetitive rinsing with de-ionized water. Solution C was prepared with equimolar mixture of 1 ng/ml BSA and myoglobin in 540 ml deionized water. This solution was formed to assay protein binding to two different types of cavities on gold target plate surface. Wells on the target surface were divided into four quadrants (Figure 2) and wells in row I(1–24) to P(1–24) were dipped for 10–15 minutes in solution C. The surface was rinsed gently and allowed to dry using nitrogen gas. Then 1 µl of SA matrix is added to the target and allowed to dry. Well in row A(1–24) to H(1–24) were used for control studies. The target plate was then used for MALDI- TOF analysis.
HAPLN1 Sensor Fabrication
HAPLN1 protein is over expressed in the majority of mesotheliomas and hence being considered as a potential biomarker for cancer detection. In a clean glass container 20 µg of HAPLN1 (64.68 kDa) protein was dissolved in 10 ml of de-ionized water (i.e 0.03 µM) and 4 mg 11-mercapto-1-undecanol was dissolved in 10 ml ethanol (i.e 0.1 mM). A mixed solution is formed, by dissolving both the solutions in 19∶1 ratio (water∶ ethanol), such that the final concentration of thiol in the solution becomes 0.1 mM. Three gold electrodes A, B and C were dipped in the solution for 12 hours and then the electrodes were rinsed thoroughly with de-ionized water to remove bound protein on the surface.
Sensor response to HAPLN1 spiked serum samples
HAPLN1-imprinted electrodes were also used for potentiometric response to the cancer biomarker spiked in serum. The 100 nM stock solution in 1∶8 serum and buffer ratio was prepared by diluting 100 µl of human serum with 650 µl of 1X PBS solution and with 250 µl of the commercial 102 µg/mL HAPLN1 solution (i.e. a total of 25.5 µg of HAPLN1). The HAPLN1 concentration in the stock solution was then 6.5 µg/ml (100 nM). 10 µl of solution was used for titration in 10 ml PBS buffer, and the highest concentration of protein is 10−9 M. Remaining standards were prepared by maintaining the same dilution rate (1∶8) of serum in buffer but analyte concentration was reduced ten times with serial a 6.5 µg/ml (100 nM) standard spiked solution.
Optimization of parameters for BSA Sensor
The BSA protein molecules were imprinted with the hydroxyl terminated alkane thiols on the gold surface. As thiols form an organized monolayer with time on the gold surface it was crucial to study the incubation time of the gold coated silicon chip inside the protein-thiol solution to achieve stable imprints. In this experiment the BSA concentration in the mixed solution was 30 µg/ml and Figure 3a shows the effect of incubation time on the stability of the imprints. Three electrodes were prepared by keeping the chips inside the solution for 2, 6 and 12 hours. The mixed solution in this experiment had a ratio of 18∶2 (water∶ ethanol) with 0.2 mM 11 mercapto1-undecanol. All measurements were done in Phosphate buffer saline (pH 7.4) in a 10 ml solution. A logarithmic increase in potential was observed with increasing BSA concentration in buffer only with the electrode, which was incubated for up to 12 hours. The results indicated that more stable imprints were formed when chip were kept in solution for 12 hours. Experiments were then conducted to optimize the conditions to achieve stable protein imprints. The potentiometric response was studied by varying the concentration of BSA imprinted on the electrode to form the cavities. In this experiment two electrodes were prepared using two different concentrations of 30 µg/ml and 1 ng/ml BSA in mixed solutions of protein and thiol.
A. Effect of immersion time of 2 hrs (___), 6 hrs (…) and 12 hrs (---). B. Effect of protein concentration of 30 µg/mL (▴, x) and 2 ng/mL (◊,△). C. Effect of protein concentration of 10 ng/mL (□) and 1 ng/mL (▴) and controls on SAM only (x, ◊).
These mixed solutions were prepared with a 19∶1 ratio (water∶ ethanol) having a final concentration of 0.1 mM 11-mercapto-1-undecanol. The incubation time was kept at 12 hours, as this was shown to support the formation of the most stable imprints.
As shown in Figure 3b, a logarithmic increase of the change in potential takes place with increase in protein concentration. The response of electrode prepared with 30 µg/ml protein imprinting concentration is much higher compared with the other electrode prepared with a lower protein imprinting concentration. This demonstrates that the number of cavities formed on the surface increases as more protein is adsorbed on the surface. The isoelectric point of a protein is defined as the pH at which the net charge on a protein surface is zero. Since BSA molecules have an isoelectric point of 4.7 they have a net negative charge in a buffer solution with pH 7.4. As more charged molecules bind to the electrode surface it leads to a drastic change in potential. The results were successfully repeated for confirmation. Figure 3b also demonstrate a higher potential change with a 19∶1 (water∶ ethanol) ratio when compared to a 18∶2 ratio from Figure 3a. This result indicates that more cavities are formed as the thiol concentration in the solution is reduced. To downscale the process and prepare more sensitive electrodes, low imprinting concentrations of 1 ng/ml and 10 ng/ml were selected based on previous experimental data. Experiments were conducted with gold electrode of much smaller surface area (2 mm2). The working electrode was prepared in a 19∶1 (water∶ ethanol) ratio with a final concentration of 0.1 mM 11-mercapto-1-undecanol. As shown in Figure 3c the electrode shows a good binding response of 15–20 mV with very low concentrations of BSA when compared to a low binding response of 2–4 mV observed with prepared control electrodes which only have the SAM on their surface.
Validation using MALDI-TOF
In this experiment 1 µl of matrix was placed on the top of each well of the imprinted Maldi plate and allowed to dry. Maldi plate analysis software with an algorithm to collect data with a scan rate of 2000 laser shot/s for each spot in a well was selected. Since half of 384 wells on the maldi plate were imprinted BSA and the other half with myoglobin protein, randomly five wells were selected from myoglobin imprinted wells and similarly five were selected from the BSA imprinted wells, to assay the protein bound to the cavities. As shown in Figure 4, three-character symbol m/z in x-axis is used in mass spectroscopy to denote the dimensionless quantity, formed by dividing the mass of an ion in unified atomic mass units by its charge number.
A. Myoglobin imprinted wells and B. BSA imprinted wells.
Y-axis show the signal intensity, but instead of using counts per second (cps), the common procedure is to relate the intensity to relative abundance with a use if an internal standard. As shown in Figure 4a myoglobin which has a molecular weight of 17 kDa was observed to be bound to only myoglobin imprinted wells as a peak appears at 17 kDa for each well and no myoglobin was observed in BSA cavities as shown in Figure 4b. BSA was observed to be of too large molecular weight under these ionizations conditions.
After the BSA studies for optimizing the conditions required for imprinting biomacromolecules, HAPLN1 imprinted electrodes were fabricated for low concentration detection of the biomarker. Three imprinted electrodes were studied to verify the response after detection. Two independent experiments show a drastic 25 mV change in the potential at very low HAPLN1 concentrations of 10−12 M as the HAPLN1 was titrated into the buffer solution (Figure 5a). As a control, BSA was also titrated under the same conditions showing a very low potential response at high BSA concentrations. The experiment was then conducted in serum with a spiked HAPLN1 concentration. Figure 5b shows that the imprinted electrode has a high drift in serum but shows a remarkable potential change at 10−9 M concentration of HAPLN1 in serum.
The detection of cancer biomarkers with a low cost and easy-to-use method is important for continuous monitoring of multiple markers. HAPLN1 is an example of increasing importance in the field as for instance Nelson et al  recently completed a transcriptome analysis on blood vascular (BEC) and lymphatic endothelial cells (LEC) and the clustering analysis showed clearly differential gene expression for BECs and LECs. HAPLN1 was second highest expressed gene in LECs and as tumors take the advantage of these metastatic potential enhancing molecules, the observed high HAPLN1 expression can lead to new therapeutic treatments for metastasis of cancer to lymph nodes. Two-dimensional molecular imprinting technology has been successfully used to imprint and detect small molecules – like peptides but imprinting macromolecules remained a challenging task. Biological macromolecules like proteins have high molecular weight and therefore their large size hinders the adsorption on the surface and the removal from the ready-made imprint –.
The large number of binding sites and functional groups on the protein surface can increase problems related to non-specific binding with molecularly imprinted proteins (MIPs) leading to poor selectivity . Therefore it is important to optimize all the required parameters and conditions to maintain the stability of such large structures throughout the process of imprinting. We selected BSA protein (65 kDa) for optimization process due to its similar size to cancer biomarker candidate HAPLN1. In Figure 3a and Figure 3b more stable imprints are formed with 12 hour incubation time and an increase in response occurs when more target molecules are imprinted at 30 µg/ml compared to 1 ng/ml concentration. Similar conditions were used by Wang et al  to show how imprints formed with different size proteins are highly specific to their respective imprint electrode using potentiometric detection. MALDI TOF technique has been used as a novel method in our work to confirm the specificity of the imprint site, also shown by potentiometric detection. Figure 4 shows myoglobin proteins bind specifically to its imprinted cavities and not to the BSA cavities. We believe that since myoglobin proteins are much smaller in size (17 kDa) compared to BSA, myoglobin would not fit in the BSA mold and hence not binding to larger BSA cavities. Results of BSA specificity could not be confirmed with MALDI-TOF due to the poor sensitivity of the equipment towards high molecular weight proteins. We have used potentiometric detection for optimization of cavities with BSA and for detection of HAPLN1 in buffer and in spiked serum. Our results in Figure 5a show 25 mV response of HAPLN1 to HAPLN1 imprinted electrode as compared to low response by BSA protein in buffer and similar results were obtained in spiked serum. Although we show the potentiometric response obtained is specific to the protein imprinted and is a result of protein binding, a clear understanding is currently lacking for the electrochemical signal (potential change) induced by protein adsorption. Several mechanisms have been suggested including by Thompson et al. He proposes factors, which might contribute to change in work function  of gold when protein binds to the gold surface and thus show how change in work function results in change of surface potential. In future experiments we plan to further check specificity of HAPLN1 protein binding to imprint cavities by surface plasma resonance technology (SPR) and also to obtain clinical data for validation.
Our data demonstrate the use of SAM forming water-soluble hydroxylated alkanethiols to imprint biomacromolecules. BSA was shown to be an effective model protein to optimize the control parameters such as protein concentration, ratio of the molecular components, and the incubation time of the chip to downscale the process of imprinting for detection of low concentrations of proteins. Molecular Imprinting based biosensor was successfully built to recognize malignant pleural mesothelioma cancer biomarker HAPLN1 and demonstrated high sensitivity by detecting low concentration of biomarker in serum/buffer solution. The sensor showed a limit of detection of picomolar concentrations with a response time of 2–5 minutes. The low cost of the sensor is based on the lack of expensive monoclonal antibodies, additional labeling molecules and on the simplicity of the open circuit potential measurement with a potentiometer, which also is very easy to use (similar to pH meter).
Conceived and designed the experiments: KL AM. Performed the experiments: AM. Analyzed the data: AM SB TN KL. Contributed reagents/materials/analysis tools: CG HP. Wrote the paper: AM KL.
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domain: biology


Several of us are away at the Somerset Folk Harp Festival, and we may not be able to respond to emails and phone calls as quickly as usual. Thank you for your patience!
This is the final post in our harp amplification series, which covers the details of connecting everything together. If you can make it through all of them, you should have a pretty complete (if basic) picture of what you’ll need to get the sound from your harp out to the ears in the back row. If you’re new to the world of amplification, we recommend reading the first, second and third posts before reading this one.
Connecting to an amp
An amp is likely to have a ¼-inch input meant to accept a signal from an instrument pickup. So if you have a pickup and an amp, it is fairly simple to connect the two with an instrument cable, plug in the amp to a power source, and start playing.
If you are using a microphone with an XLR connector, check whether your amp also has an XLR input. It would look something like this:
If it does, you should be fine plugging your mic into the amp directly with an XLR cable. If it doesn’t, there are transformers you can get to change the signal from balanced to unbalanced and to adapt the plug so it fits into the amp’s ¼-inch input. If you have a choice, an amp with an XLR input is the better option, since it will have a preamp built in that is intended to receive a microphone signal.
Connecting to a PA
When connecting to a PA system, you will probably want to use XLR cables. Even if the mixing board can accept ¼-inch cables, XLR is better for covering long distances because there is less loss of signal. If you have a microphone, you can connect it directly to the PA’s mixing board with an XLR cable. If you have a pickup, you’ll need to convert from ¼-inch to XLR, and the best thing to use is a D.I. (see below).
Preamps and D.I.s – what are they and do I need one?
D.I.s, or direct-in boxes, convert signal from unbalanced to balanced, as well as adjusting the signal strength. They are quite often used when instrumentalists with pickups are plugging into a PA through a mixing board. The D.I. has an input jack to accept a ¼-inch cable (coming from an instrument pickup) and an output jack to accept an XLR cable (going to a mixing board). Many instrumentalists who need to plug their pickup into a mixing board will have their own D.I., and there are plenty of inexpensive options out there. If you are using your own amp, it will most likely have a ¼-inch input and you will not need a D.I.
A preamp gives your signal a boost between the pickup and the amplifier, allowing your amp to put out a greater volume of sound. Many pickup/amp combinations produce enough sound without one, but if you are playing harp with a rock band and need to be heard over a drum set and a screaming guitar, using a preamp to boost your volume might not be a bad idea. Generally, you would use one ¼-inch cable to connect the pickup to the preamp, and a second ¼-inch cable to connect the preamp to the amp.
Some preamps have volume and/or tone controls built in, which allow you more freedom to adjust your sound without needing to reach your amp. The L.R. Baggs Gigpro preamp is an example of this style. [Edit: The Gigpro is no longer made.]
There are also D.I./preamp combinations that cover all the bases, boosting the signal as well as converting from unbalanced to balanced if you need it, and often including a greater variety of tone controls. They are generally more expensive, and probably not something you need right off the bat. However they do provide almost anything you might ever want in a performance situation in a single device. The L.R. Baggs Para Acoustic D.I. is one example.
If you made it this far, congratulations! Hopefully you’re feeling a little more comfortable with the jargon and have figured out what questions to ask next. If we can help with anything specific relating to the products we sell, please give us a call. If you have any thoughts or experiences to share, we welcome you to leave a comment below.


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Everybody always talks about M Night Shyamalan's surprise endings. But the shocker here is the opening. What on earth is this hip, contemporary director doing making a period film? Set among some fearsome woods, The Village is entirely cut off from the rest of the world - which makes it hard to say exactly what period we're supposed to be in. But there's no electricity, no plumbing except the well, and the Villagers wear felt capes and tunics. It's somewhere between The Crucible and Little Red Riding Hood.
The Village people are a rum lot. They include Joaquin Phoenix as Lucias, whose mother, Sigourney Weaver, is one of the council of elders, and who seems to have a thing for the leader, Edward, played with characteristic solemnity by William Hurt. (Anyone remember The Janitor, 20 years ago now?) Quasi-incestuously, Lucias fancies Edward's blind daughter, Ivy (Bryce Dallas Howard, whose real-life dad is director Ron Howard). His main rival is Adrien Brody's Noah, and he's nutty as a fruit bat, so things are looking good for Lucias.
The first half of The Village is intriguing, to put it mildly. As The Sixth Sense showed and Signs confirmed, Shyamalan is a master at building atmosphere, and here he lays it on with the proverbial trowel. When we learn what manner of beasts is keeping the community fenced in, the movie is only a whisper away from camp. But there's a kind of delirious riskyness to the conceit which is quite invigorating - you can feel how desperately Shyamalan wants to believe in monsters, which helps to go along with it.
The love story is also well observed, and touchingly played by Howard and Phoenix. And there's a brilliantly directed twist which I wouldn't dream of giving away. But mind, this is all in the first half of the movie.
Critics were pretty sniffy about The Village when it came out in cinemas. Although I think the critics were harsh, and there are some pretty scary sequences, I would have to agree that the second half is a bit of a let down, especially the ending. For a start, it's a twist you can guess, which is disappointing. And you've probably seen it before, which is disastrous. It's also, I think, the wrong ending. Shyamalan clearly means the film to be taken as an allegory for isolationist Fortress America.
For most of the movie his sympathies are clearly with the young people who kick against that deeply conservative thinking, and who bravely risk everything to confront superstition and lies. Without giving anything away, I believe the ending betrays those characters' courage and identifies the wrong villain. It's a failure of nerve on Shyamalan's part, and that may be what really cost him at the box office.
The DVD, which includes a reasonably interesting 'making of' documentary, doesn't address any of these issues, which is a shame. The Village is a better movie than it was given credit for. But I had hoped there might have been an alternate ending hidden away somewhere in those deleted scenes.


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The death of American Idol Season 8 contestant Alexis Cohen is being investigated as a vehicular homicide. New Jersey police arrested 23-year-old Daniel Bark on Sunday in connection with Cohen’s death. The body of the former American Idol contestant was found lying in a street in Ocean County on Saturday.
According to police authorities, Alexis Cohen was struck and killed by a car around 4 a.m. on Saturday. An autopsy showed 25-year-old Cohen suffered massive trauma, including injuries to her chest, abdomen and head. Cohen may have been attempting to return to her car, which was found nearby, when she was hit while crossing the street.
Ocean County Deputy Chief Prosecutor Michael Mohel said Daniel Bark was arrested around 6 p.m. on Sunday after an “extensive investigation,” according to the Asbury Park Press. Bark has been charged with reckless driving leading to the death of Alexis Cohen and leaving the scene of a collision. Bark could face up to 15 years in jail if convicted.
Alexis Cohen famously gave an impassioned, vulgar rant to American Idol cameras after failing to receive a golden ticket to Hollywood in the auditions for Season 7. The following year, Cohen auditioned again for Season 8, but failed to impress the judges a second time.
The Alexis Cohen rant YouTube video became a fan favorite following her first appearance on American Idol, briefly launching the young aspiring singer into the national spotlight.


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Cold Feet: Jenny talks to Charlie about her cancer treatment
ITV comedy-drama Cold Feet was a huge hit during its original run between 1998 to 2003. There were five seasons and the show won critical acclaim both in the UK and abroad after it was nominated from an International Emmy Award. Cold Feet sent the star statuses of its central cast skyrocketing – Express.co.uk takes a look at what the cast are doing now.
Where are James Nesbitt and the Cold Feet cast now?
James Nesbitt was undoubtedly one of the biggest stars of Cold Feet as the lovelorn Adam Williams.
Since the show ended, he has consistently remained on screens with roles ranging from Ballykissangel, Jekyll and Murphy’s Law.
More recently, he has appeared in Stan Lee’s Lucky Man, The Secret and The Missing – all of which have won him critical acclaim.
He also reprised his role in Cold Feet when Mike Bullen decided to revive the series in 2016.
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Robert Bathurst was better known as David Marsden, who ended up having a tumultuous marriage which eventually ended.
After Cold Feet wrapped up, Bathurst went on to have a successful career and appeared in 2009’s Emma, The Pillars of the Earth, Dracula and Wild At Heart.
He also saw his stardom soar after appearing in Downton Abbey as Sir Anthony Strallan, who famously jilted Laura Carmichael’s Lady Edith Crawley at the altar in a shocking storyline.
Some of his other roles include appearing in TV shows Blandlings, Cockroaches and Toast of London as well as returning to Cold Feet.
John Thomson portrayed Peter Gifford, whose marriage came to an end after much heartache.
Thomson’s post-Cold Feet roles include Blackpool, New Street Law, After Hours and The Moonstone.
As well as coming back to Cold Feet for the revival, Thomson was most recently on The Masked Singer UK.
He was singing under the guise of the super-cute Bush Baby and when he was finally unmasked, the judging panel hailed his singing abilities.
Cold Feet 2020 location: Where is Cold Feet filmed? Where’s it set? [EXPLAINER]
Cold Feet 2020 cast: Who is in the cast of Cold Feet? [INSIGHT]
Cold Feet star John Thomson speaks out on show return ‘Not cancelled’ [INTERVIEW]
Hermione Norris was Karen Marsden, who was originally married to David but the pair went their separate ways eventually.
Norris has take on a plethora of roles from spy thriller Spooks to detective drama Wire In the Blood to period piece The Crimson Field.
In recent years, she played the sinister wife of a serial killer in Luther as well as starring in ITV drama Innocent and baby series In the Club.
She took came back to Cold Feet and will next be starring in Between Two Worlds.
Fay Ripley was Jenny Gifford, who was married to Peter but their union wasn’t fated to last.
Ripley has featured in an array of projects including Monday Monday, Reggie Perrin and The Delivery Man.
Helen Baxendale left Cold Feet as Rachel Bradley after her character was killed off in a shocking storyline.
The star found international fame after appearing in the American sitcom Friends and the time but seems to have had a lower profile than her co-stars.
She appeared in Kidnap and Ransom and more recently Cuckoo and Noughts + Crosses.
The Graham Norton Show airs on BBC One tonight at 10.45pm
Source: Read Full Article

domain: celebrity


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St. Paul, Minn. — Election Day turned out to be a pretty good one for schools. Most of the ballot questions across the state that asked for some form of local funding were approved, even though in many cases, that now means those voters' property taxes will increase.
Of the 93 school funding referendums on ballots across the state yesterday, voters approved at least 51 of them and rejected 26. Some results still weren't known. But the mostly positive results surprised some school leaders, especially considering the bad economy.
Some votes were seeking money to pay for construction – either to fix existing schools or build new ones. Those efforts passed in Hastings and Wayzata, but failed in Menahga and Hermantown.
Hermantown, in fact, was asking for more money than any Minnesota district and it lost by the widest margin of all school questions in the state. Nearly 82-percent of Hermantown voters rejected the $58 million effort, a message that superintendent Brad Johnson said he hears loud and clear.
"From what I've heard from people, the complaints and concerns that were expressed to me was it was too big and too much," Johnson said. "That's why we took it to the community for a vote in the first place. We wanted to see what their thoughts were."
Construction, though, was not the goal for most district. Most were asking voters for local operating funds to make up for relatively flat funding from the state.
In most cases, those pitches came with a tax increase, but still won approval in International Falls, Spring Lake Park, St. Francis and South St. Paul, to name a few. Places where they failed include Moorhead, Austin, and North Branch.
A close look at the results reveals a key trend. Voters were much more generous when districts only asked to renew an existing tax that was about to expire. That tactic allowed schools to say their proposals were not a tax increase. Rather, they were more of a tax continuation.
Some of the highest percentage of 'yes' votes in Minnesota came from districts that were only seeking renewals. In Aitkin, 84 percent voted yes. Maple River and Blue Earth both saw 82 percent. Voters in the Buffalo-Hanover-Montrose district rejected a tax increase last year but approved yesterday's renewal of an earlier levy. The state's largest district, Anoka-Hennepin, also gained a renewal.
In the town of Holdingford, superintendent John Haas is convinced the 'renewal only' approach is why his district won over voters.
"We've gone through a period of rather severe cuts to get out of statutory operating debt. So, I think most people acquainted with our situation realized that we were just trying to keep the status quo and not really add anything," Haas said.
For many districts, going to voters is becoming an almost annual event. Rockford voters approved yesterday's measure on what was the district's fifth try this decade. Pelican Rapids lost a tax hike vote last year by just 144 votes, but this year's questions failed by even larger margins.
And then there's the Perham district in west-central Minnesota. After last year's overwhelming rejection at the polls, the district closed a school in the town of Dent to save money. This year's vote was much closer - but still ended in defeat.
Superintendent Tamara Uselman says that will mean cutting another million dollars, though she's at a loss for how to do that. Uselman says a bad economy is a tough time to ask voters for more money, but she also says that's kind of the norm.
"I don't know very many times when schools need to go out and ask for money in a good year," Uselman said. "If economic conditions are good, the state funds schools well and you don't need to be asking for money."
Uselman said she's ready for the challenge of making more cuts and is trying to keep things in perspective. It will ultimately be up to the school board to decide whether to try again with voters in the future.