Of course, that does not make maintenance and upgrade work any less essential. In fact, Cook stated clearly that “asset sustainability has been a really big risk” for rail infrastructure in the UK and that “the long-term cost of keeping it in a steady state was going up”. Indeed, recent data from the ORR showed that payments from Network Rail – the owner and manager of the UK’s rail infrastructure – to train operators for planned and unplanned disruption rose by £52m in 2017/18, totalling £439m for the year.
It’s not just the UK. Planned and unplanned maintenance is a huge cost for rail operators around the world. However, while specialised devices have enabled a certain level of rail monitoring, these devices are limited operationally – generally offering monitoring at a single fixed location. As such, DAS is a technology that has promised significant gains when it comes to optimising rail maintenance processes and delivering cost savings to operators.
Interestingly the capabilities of DAS in rail applications have moved on quickly. At a recent event, hosted by Frauscher, a number of operators offered their insights on the results they have achieved with DAS systems.
For example, the Austrian rail operator ÖBB described how it has used DAS to aid the detection and location of flashover events in the overhead catenary cables.
In 2016 ÖBB recorded nearly 4,000 short-circuits across its 8,100km network. Whereas conventional technologies cannot accurately detect the location of the short circuit, research with DAS has shown that using an optical fibre installed parallel to the line, shorts can be pinpointed to within 10 metres – even when a train is passing. This has the potential to dramatically accelerate repairs, and therefore minimise passenger delays, in what is a regular source of disruption.
Another example of the value of DAS came from South America, where rail conditions can often be poor – especially where the rail infrastructure is used to support the mining industry. However, a local operator established that DAS can detect a large range of faults including loose sleepers, loose track ties, rail squat and rail break.
The initial push for DAS in the rail sector was entirely focused on train tracking and detecting rail break. However, the progress made in the last few years has exceeded even our own expectations for the technology. As the examples above demonstrate, DAS can now deliver a much more all-encompassing level of rail monitoring – identifying a range of failures and enabling more strategic, smaller, ongoing, and more proactive maintenance, rather than only knowing there is a problem when something major fails.
These advances have been made possible by our own technical developments. Innovations like our Helios Theta platform offer exceptional high resolution monitoring – being able to identify faults to specific bogies on a train for example – but crucially this high resolution monitoring can cover over a range of 40km. For large scale infrastructure like railways, this extended range is a game changer.
These technical advances are making a compelling case for the broader adoption of DAS – and it is poised to make a huge impact in the coming years. Operators around the world are proactively identifying priority applications for the technology and a new industry body (FOS4R – Fibre Optic Sensing for Rail) created by operators will help promote the benefits further. Having gone far beyond its original purpose, DAS will soon be a big part of delivering better railways, more efficiently and with less passenger disruption. A true win-win for the industry and passengers.


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Taste- in praise of the seasonsTASTE is our new cookbook with inspiring recipes and beautiful photographs intended to tempt you to have fun in the kitchen. The instructive recipes are easy to follow and will help you succeed in the kitchen.How about starting with crispy goat cheese wraps, moving on to chilli-hot autumn chicken and rounding this off with a wonderful chocolate cake?TASTE contains recipes for every season and every Occasion!
Taste : in praise of the seasons
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Songs and you will thinking has good bi-directional matchmaking
With respect to your own experience of music throughout the perspective out of mental regulation, there have been two secrets to adopt cognitive reappraisal and you can expressive suppression
- Awareness: Discover bodily feeling, where they happens, why it takes place. Learn to articulate her or him into the words and you will establish an experience.
- Access: Play with solutions to publication their awareness of work at real feelings. You could begin because of the are aware of the breathing and brand new relevant human anatomy path. You’ll be able to concentrate on the improvement in their muscle tissue tension. You can publication the hand to the touch and you may experience their interior feel regarding exterior. Including, coming in contact with the fresh new tensed upwards muscle tissue with your hands can be make an enthusiastic attention to the proceedings. Muscles relaxation try a key facet of regulating feelings with an anxiety role. Immediately after which is complete, the initial action should be to maintain your awareness for the reason that it occurs when many people understand new stuff regarding the themselves otherwise its experience.
- Reappraisal: Reassess their sense and you can state to modify your reaction to the brand new feel. This concerns a myriad of cognitive reappraisal also semantic conversion process, third-people mind-talk, and guiding your own awareness of focus on compatible information.
In terms of speaking about state-of-the-art emotions, there clearly was a detachment amongst the thinking along with your attention to all else, as well as your system. MABT might help treat that it disengagement and move they into a great body-feelings involvement.
A related mental regulation expertise are mindfulness. Research shows you to definitely mindfulness degree is useful from inside the deterioration a fear response. On the data, professionals educated a lowered anxiety response through mindfulness-yoga meditation including attentional control and you will sensory feel. Yoga, as an over-all pastime, is actually obviously useful in improving the total well being and you may emotional well-becoming.
5. Tune in to sounds
The option of audio is essential. Although not from the old-fashioned Category feel. Hearing musical you’re a fan of possess a lot more advantages. Instance, while you are a fan of heavy metal and rock audio, heavy metal makes it possible to techniques frustration constructively.
When you are impression sad and also you pay attention to sad sounds, the fresh congruence between your emotion and you may disposition can help with controls. Unfortunate audio will not necessarily result in depression. In reality, it creates united states have more confidence Search factors to step 3 preferred aftereffects of playing sad musical sweet sorrow (confident emotions on the depression), uplifting and you can calming sorrow (improves spirits), and you may legitimate depression (an experience of depression).
The option of music may affect every day and your feeling can impact your choice. Most people provides an user-friendly feeling of what they need away from audio. Very, this is exactly a bit an easy way to control attitude.
Intellectual reappraisal feels like the new semantic transformation technique for which you transform the manner in which you translate and you can procedure psychological viewpoint. Expressive suppression is changing the fresh new behavioural reaction (snapping, withdrawing, tantrums, etc.) by knowingly closing an undesirable reaction. Studies have shown one tunes advances really-are when https://datingranking.net/it/sesso-occasionale/ it is utilized due to the fact a difficult control means. However, only if having fun with intellectual reappraisal. It can features negative effects towards better-becoming should your wade-to method is expressive inhibition.
Sounds can also be try to be a great distraction which help point your self of the newest emotionally loaded perspective. It makes your introspect, that’s an important position for intellectual reappraisal. Moreover it promotes relaxation and you will glee. Furthermore, music tends to be a fantastic partner with other emotional regulation experiences. Such things, predicated on look, influence thinking-control, emotional regulation, cognitive regulation, and well being.
What about mental regulation for the traumatized students? It can be tough to illustrate them when you look at the breathing and you can third-individual care about-speak, what exactly are you willing to would? The solution can be music, once more. Songs might be a passively created energetic sort of psychological regulation for the kids.

domain: psychology


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Breaking: DSS Confirms Plot To Put A Halt To Tinubu’s Swearing In, Unveils Next Line Of Action
The Department of State Services (DSS) on Tuesday disclosed that it has linked some key people plotting to scamper the May 29 handover to a new government and force an interim government in Nigeria.
According to the DSS in a statement by its spokesperson, Peter Afunanya, the plan by the identified people is to set aside the constitution and undermine civil rule, sponsor mass protests as well as plunge the country into an avoidable crisis in order to pave way for the planned interim government.
The statement added that the individuals involved in the plot to obtain frivolous court injunctions to forestall the May 29 inauguration of new executive administrations and legislative houses at the Federal and State levels.
The DSS however promised that it would work hand-in-hand with all relevant organizations, individuals, and groups to ensure a seamless and successful inauguration on May 29, 2023, as promised by President Muhammadu Buhari to hand over to a new administration of the President-elect, Bola Tinubu.
The secret police, therefore, warned those plotting to undermine democracy in the country to refrain from their devious schemes and orchestrations.
It also warned stakeholders including judicial authorities, media, and the civil society to be watchful and cautious to avoid being used as instruments to subvert the peace and stability of the nation.
The DSS promised to continue watching and not hesitant in taking decisive and necessary legal steps against these misguided elements to frustrate their obnoxious intentions.
Below is the full statement.
“The Department of State Services (DSS) has identified some key players in the plot for an Interim Government in Nigeria. The Service considers the plot, being pursued by these entrenched interests, as not only an aberration but a mischievous way to set aside the constitution and undermine civil rule as well as plunge the country into an avoidable crisis. The illegality is totally unacceptable in a democracy and to the peace loving Nigerians.
“This is even more so that the machination is taking place after the peaceful conduct of the elections in most parts of the country. The planners, in their many meetings, have weighed various options, which include, among others,to sponsor endless violent mass protests in major cities to warrant a declaration of State of Emergency. Another is to obtain frivolous court injunctions to forestall the inauguration of new executive administrations and legislative houses at the Federal and State levels.
“The DSS supports the President and Commander-in-Chief in his avowed commitment to a hitch-free handover and will assiduously work in this direction. It also supports the Presidential Transition Council and such other related bodies in the States.
“It will collaborate with them and sister security and law enforcement agencies to ensure seamless inaugurations come 29th May, 2023.
“Consequently, the Service strongly warns those organising to thwart democracy in the country to retract from their devious schemes and orchestrations.
“Stakeholders, notably judicial authorities, media and the Civil Society, are enjoined to be watchful and cautious to avoid being used as instruments to subvert peace and stability of the nation.
“While its monitoring continues, the DSS will not hesitate to take decisive and necessary legal steps against these misguided elements to frustrate their obnoxious intentions.”
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text:
PDF KINDLE [Iris Murdoch Work for the Spirit]
Spooky She s nailed IM This is the best book on
great Dame little mainly lit crit Other worthy biographers are P Conradi A Byatt F White. Iris Murdoch Wikipedia Iris Murdoch and Her Work | Columbia University This book explores different aspects of Murdoch's work including her philosophy and fiction focusing on a wide variety of issues ranging from reading Murdoch as a fabulator to the central role Murdoch plays in the ethical turn Under the Net fr Murdoch Iris Livres anglais et Iris Murdoch – was born in Dublin and brought up in London She studied philosophy at Cambridge and was a philosophy fellow at St Anne's College for years She published her first novel in and was instantly recognized as a major talent Iris Murdoch accueil databnffr Iris Murdoch and her work Iris Murdoch La romancire anglaise Iris Murdoch publieThe Great Dame Little
en france son vingtime roman message de laFrance son vingtime roman Message de la aux ditions Gallimard; en mme temps parait en Angleterre son ouvrage de philosophie Metaphysics as a guide to morals Iris Murdoch chez elle dans sa maison d'Oxford Photo Sophie Bassouls Sygma Cote Iris Murdoch on making your unconscious work for Iris Murdoch – “I live I live with an absolutely continuous sense of failure” the struggling writer narrator of Murdoch’s novel The Black Prince declares in a confrontation with his literary rival about midway through the book “I am always defeated always Every book is the wreck Iris Murdoch | Biography Books Facts | Britannica Iris Murdoch in full Dame Iris Murdoch original name in full Jean Iris Murdoch married name Mrs John O Bayley born July Dublin Ireland died February Oxford Oxfordshire England British novelist and philosopher noted for her psychological novels Art as Pure Villain On Iris Murdoch's The Sea The Today July would hav. B Nicol She often compares the novels presenting IM s development her ideas There s amazing continuity from
them Just what IM achieved with her. E been Booker Prize winning andJust what IM achieved with her. E been Booker Prize winning novelist and Iris Murdoch’s st birthday To mark the occasion here’s a review of her most famous work The Sea The Sea Analysis of Iris Murdoch's Novels | Literary Theory A nowledge of Iris Murdoch’s July – February philosophical and critical essays is invaluable for the reader wishing to understand her fiction Her moral philosophy which entails a rejection of existentialism behaviorism and linguistic empiricism informs her fiction throughout and provides a basis for an interpretation of both the content and the form of her work Iris Murdoch at ‘Her books are full of passion Iris Murdoch | Books | The Guardian Iris Murdoch Writers and Their Work poche Achat Livre Iris Murdoch Writers and Their Work Des milliers de livres avec la livraison chez vous en jour ou en magasin avec % de rduction Iris Murdoch work for the spirit eBook Originally published in this brilliant study provides a perceptive and up to date assessment of the novels of Iris Murdoch up to and including Nuns and Soldiers published in The Fire and the Sun her book on Plato is also considered in depth Iris Murdoch Philosopher Oxford Scholarship This book offers a detailed introduction to Iris Murdoch's philosophical work especially the moral philosophy of The Sovereignty of Good Murdoch argued for an important and distinctive position in opposition to the mid‐th‐century analytic philosophers like R M Hare and Stuart Hampshire and to existentialists like Sartre Iris Murdoch Books Biography and List of Iris Murdoch – Dame Jean Iris Murdoch July February was an Anglo Irish novelist and philosopher famed.

domain: literature


text:
Day Fifty-Four Reading – Psalms 126-131
Psalms 126 KJV
1 When the Lord turned again the captivity of Zion, we were like them that dream.
2 Then was our mouth filled with laughter, and our tongue with singing: then said they among the heathen, The Lord hath done great things for them.
3 The Lord hath done great things for us; whereof we are glad.
4 Turn again our captivity, O Lord, as the streams in the south.
5 They that sow in tears shall reap in joy.
6 He that goeth forth and weepeth, bearing precious seed, shall doubtless come again with rejoicing, bringing his sheaves with him.
1 Except the Lord build the house, they labour in vain that build it: except the Lord keep the city, the watchman waketh but in vain.
2 It is vain for you to rise up early, to sit up late, to eat the bread of sorrows: for so he giveth his beloved sleep.
3 Lo, children are an heritage of the Lord: and the fruit of the womb is his reward.
4 As arrows are in the hand of a mighty man; so are children of the youth.
5 Happy is the man that hath his quiver full of them: they shall not be ashamed, but they shall speak with the enemies in the gate.
1 Blessed is every one that feareth the Lord; that walketh in his ways.
2 For thou shalt eat the labour of thine hands: happy shalt thou be, and it shall be well with thee.
3 Thy wife shall be as a fruitful vine by the sides of thine house: thy children like olive plants round about thy table.
4 Behold, that thus shall the man be blessed that feareth the Lord.
5 The Lord shall bless thee out of Zion: and thou shalt see the good of Jerusalem all the days of thy life.
6 Yea, thou shalt see thy children’s children, and peace upon Israel.
1 Many a time have they afflicted me from my youth, may Israel now say:
2 Many a time have they afflicted me from my youth: yet they have not prevailed against me.
3 The plowers plowed upon my back: they made long their furrows.
4 The Lord is righteous: he hath cut asunder the cords of the wicked.
5 Let them all be confounded and turned back that hate Zion.
6 Let them be as the grass upon the housetops, which withereth afore it groweth up:
7 Wherewith the mower filleth not his hand; nor he that bindeth sheaves his bosom.
8 Neither do they which go by say, The blessing of the Lord be upon you: we bless you in the name of the Lord.
1 Out of the depths have I cried unto thee, O Lord.
2 Lord, hear my voice: let thine ears be attentive to the voice of my supplications.
3 If thou, Lord, shouldest mark iniquities, O Lord, who shall stand?
4 But there is forgiveness with thee, that thou mayest be feared.
5 I wait for the Lord, my soul doth wait, and in his word do I hope.
6 My soul waiteth for the Lord more than they that watch for the morning: I say, more than they that watch for the morning.
7 Let Israel hope in the Lord: for with the Lord there is mercy, and with him is plenteous redemption.
8 And he shall redeem Israel from all his iniquities.
1 Lord, my heart is not haughty, nor mine eyes lofty: neither do I exercise myself in great matters, or in things too high for me.
2 Surely I have behaved and quieted myself, as a child that is weaned of his mother: my soul is even as a weaned child.
3 Let Israel hope in the Lord from henceforth and for ever.


A MUM who gave evidence against rapist cop Stephen Mitchell has broken her silence and revealed she hopes to pursue a civil case against the police.
The police officer was accused of indecent assault and misconduct in relation to the mum but was cleared of the charges.
However the 42-year-old was convicted of sex attacks on other women he met while on duty and was last week ordered to serve two life sentences.
The mum, of Walker, Newcastle, said she was disappointed Mitchell was cleared of the charges relating to her but that she hoped to take civil action against Northumbria Police.
She said: “I was mortified that he was cleared of the charges but I’m really pleased for the girls who got guilty convictions against him.
“In some ways it feels like the whole thing has been worth it.”
She added: “I would like to take more action against PC Mitchell and the police if possible, through a civil case.”
Newcastle law firm Irwin Mitchell is already representing three women who are launching multi-million-pound compensation claims against Mitchell while they are in talks with others who are seeking to do the same.
Serious injury specialist Lindsay Houghton said: “We are making claims for sexual assault, false imprisonment and breach of human rights.
“Mitchell was in a position of trust and authority.
“He abused that position, taking advantage of vulnerable young women in the most horrific way.”
Up to 30 women could make a civil claim as 16 gave evidence against him in court and a further 14 were prepared to give evidence.
The mum-of-two added: “The feelings I have about Mitchell run so deep it’s difficult for me to talk about it.
“When I get my children up in the morning, I think to myself, ‘how can I bring them up to trust the police?’.
“I think Mitchell is a horrible, dirty, sex predator who preys on vulnerable women.
“I would call him a complete low-life coward.
“He used his police badge for his own sexual gratification, knowing it would give him access to his victims.”
It was revealed in court how Mitchell, formerly of Whitley Bay, North Tyneside, raped and sexually abused heroin addicts, shoplifters and a disabled teenager by offering them help while in custody, then demanding sexual favours afterwards.
He must serve a minimum of seven and a half years before he is considered for parole.
The mum added: “I’m pleased for the other girls but there are still some of us who feel we haven’t got justice.”


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Innovation, Inventor, Founder and Shareholder of Companies, Granted & licensed Patents in Europe, USA & worldwide with applications in improved and early diagnosis of disease (auto immune diseases & cancer)
- Dolores Cahill co-founded Protagen AG in Dortmund (https://protagen.com/) (1996-2019) as a spin-out of the Max-Planck-Institute, Berlin to commercialise her diagnostic, companion diagnostic and personalised medicine applications of her discoveries. Protagen has screened and profiled the autoantibody repertoire of over 20,000 patients (https://protagen.com/data-and-insight/scientific-publications/)
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Research, Scholarship & Innovation: Selected Granted & Licensed Patents
- Novel method for the identification of clones conferring a desired biological property from an expression library. PCT WO 09/070,590. Inventors: Dolores J. Cahill, Konrad Büssow, Wilfried Nietfield, Gerald Walter, Hans Lehrach. Exclusively licensed to Protagen AG, Dormund, Germany.
- A new method of selection of clones from an expression library involving rearraying. PCT WO 09/070,547. Inventors: Dolores J. Cahill, Konrad Büssow, Wilfried Nietfield, Gerald Walter, Hans Lehrach. Exclusively licensed to Protagen AG, Dortmund, Germany.
- Method for identifying and/or characterising a (poly)peptide. Date filed in Europe, PCT: E1131 PCT. Inventors: Dolores J. Cahill, Eckhard Nordhoff, Joachim Klose, Holger Eickhoff, Frank Schmidt and Hans Lehrach. Exclusively licensed to Protagen AG, Dortmund, Germany.
- A novel system for dual protein expression in Pichia pastoris and Escherichia coli. Date filed Europe PCT. Inventors: Angelika Lueking, Caterina Holz, Hans Lehrach, Dolores J. Cahill. Licensed to MoBiTech, Germany.
- A novel approach to generate cDNA expression libraries in yeast enriched for open reading frames (ORF). Europe PCT Inventors: C.Holz, A.Lueking, D.J. Cahill, H. Lehrach. Licensed - MoBiTech, Germany.

domain: biology


text:
Ministers in charge of some of the most problematic files for the Progressive Conservative government — including education and social services — got smaller portfolios, as did Fedeli, the minister in charge of all spending, after his budget was not well received.
The government has faced weeks of criticism as funding cuts that were hidden in the April budget have since emerged in a near-daily stream, fuelling successive waves of criticism and outrage.
Every one of our cabinet ministers, I feel, have done a good job, but we can always do a better job.Premier Doug Ford
Ford acknowledged communications problems over the past year, but framed his cabinet shake-up as a way to start fresh for year two.
“Every one of our cabinet ministers, I feel, have done a good job, but we can always do a better job,” he said.
Fedeli did “an incredible job” as finance minister, but will also be great in his new role as economic development minister since he is a natural salesperson, Ford said.
Rod Phillips, who as environment minister played a major role in the cancellation of the province’s cap-and-trade program and attacking the federal carbon tax, replaces Fedeli in finance.
Lisa MacLeod, who angered parents over her handling of the autism file as minister of children, community and social services, is taking over the tourism, culture and sport portfolio.
Lisa Thompson moves from education to government and consumer services after sparring with teachers’ unions and angering parents by increasing class sizes.
Her successor in education, Stephen Lecce, has a monumental task ahead as bargaining with teachers is just getting underway in a new era of wage restraint and the fallout of larger class sizes is looming in September.
Lecce is one of two backbenchers who got huge promotions, with Doug Downey also becoming attorney general.
In all, there are seven new faces around the cabinet table, with the size of the executive council ballooning from 21 to 28 people. As one of Ford’s first acts as premier he slashed the size of Toronto city council, but denied that increasing the size of his own government sends a contradictory message.
“We’re always going to have the best interests of running an efficient government,” he said. “If you look at some of the other cabinets over the years we’re still in line, if not smaller.”
Merrilee Fullerton, who came under fire for changes to post-secondary education as minister of colleges and universities, will be responsible for long-term care. And Caroline Mulroney is being moved out of her role as attorney general to the post of transportation minister. She will continue to be responsible for francophone affairs.
Health Minister Christine Elliott, Solicitor General Sylvia Jones and Treasury Board President Peter Bethlenfalvy are among the high-profile members of cabinet keeping their posts.
The shuffle comes just after Ford and his Tories marked the one-year anniversary of winning a majority government.
Ford has been slipping in the polls, and some suggest he could hurt Conservative Leader Andrew Scheer’s chances of winning in Ontario in the October federal election.


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Skip to comments.Scientists face four years in prison for failing to predict earthquake
Posted on 09/27/2012 11:22:44 AM PDT by Hunton Peck
Six Italian scientists and one government official could see four-year prison terms for manslaughter for allegedly downplaying the risk of an earthquake in the town of L'Aquila, Italy, in 2009.
According to prosecutors, the six researchers and the Department of Civil Protection downplayed the likelihood that a series of tremors that hit the city in early 2009 were foreshadowing a larger quake. On April 6, 2009, a magnitude-6.3 earthquake killed 309 city residents.
The trial, which began about a year ago, has worried scientists, who point out that earthquake prediction is not possible. But prosecutors insist that the trial is not about predicting the unpredictable, according to Nature News. During closing arguments on Monday and Tuesday (Sept. 24-25), the prosecution assistant told the courtroom that instead, the scientists and officials had inadequately assessed the risk of a quake and given deceptive information to the public.
(Excerpt) Read more at foxnews.com ...
So, the resulting consequences will be daily headlines of
“Scientists Predicting Dire Disaster Strikes” - of course the said disaster could happen any time in the next 5 billion years, that should about cover it.
The sky is falling crowd will be so pleased.
If I remember right, they said there was no chance for an earthquake right before it happened.
I am torn on this one. They ignored a lot of evidence, but we don’t jail the weatherman for a tornado.
The Italian justice system is nuts.
And if he weighs the same as a duck, he is a.....?
Yep. I even made fun of that and the “Wife of Jesus” nonsense on my new blog. Reposted on FR in its entirety of course.
6.3 earthquake won’t even phase a descent building!!!
Build crap in an earthquake zone and don’t bitch when you get killed!!!!
Gee - And I thought the “someone, anyone, must be held to blame” virus was a particularly American legal disease!!!
I missed that one.
Where they once burned diviners, they now punish non-divination. How insane is that?
Sorry but I don't see the distinction here. What's the difference in failing to predict the unpredictable and "inadequately assesing the risk of a quake"?
Hmmm, let’s see, the guilty GW alarmists should be...burned at the stake!
Can the families of the embassy staff sue the state department for dismissing the danger of an attack?
I guess if you’re paid to predict earthquakes and you fail to predict them then its professional malpractice in the least.
Back in the days when even lawyers and judges were mostly sane people, malpractice required more than just an unfavorable outcome. Some failure to meet accepted practice standards was also necessary. Supposedly, that’s still a requirement in American courts.
Lets see how the pols respond when scientists warn them to evacuate Naples because of the threat from the Potzzuoli super volcano...
And if they predict an earthquake and it doesn’t happen? Is that a crime too?
What’s really stupid is building again in the same place as the earthquake that just happened.
Certain areas are earthquake-prone, and there will be another earthquake, it’s just a matter of time.
The french authorities blamed a mechanic for allegedly leaving a screw loose or some trivial thing that caused the Concord disaster.European “justice” has its fair share of scapegoats for all kinds of catastrophes.So yes there is an arbitrary and informal way of randomly charging somebody if things go to pot in Europe.
The problem with Italy and it is a beautiful country is that a lot of the buildings are old fashioned and they tend to collapse during big earthquakes.An entire town had to be abandoned after it became too unstable.
that’s nearly as silly as gubmint fining people for not buying a product.
Disclaimer: Opinions posted on Free Republic are those of the individual posters and do not necessarily represent the opinion of Free Republic or its management. All materials posted herein are protected by copyright law and the exemption for fair use of copyrighted works.


text:
Join me in supporting real change, our youth is our future. I have been involved with this youth organization for over 23 years. Let's support good in the world and make a difference. Help us transform 2,500 youth into capable leaders, motivated to affect positive social change in an increasingly diverse world!
Just a small donation will go a long way to helping me meet my goal for National Teen Leadership Program!

domain: sociology


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“We are passionate about serving this very rural part of the country…we have people who really care and are really passionate about their communities, about their neighbors, about doing good things for those around them.”
“I’m Chad Olney and I’m the CEO at Pacific Crest Federal Credit Union. We’re headquartered in Klamath Falls Oregon and have two branches here. We’ve also got a branch in Alturas California, Lakeview Oregon, and Christmas Valley Oregon. We are passionate about and love serving this very rural part of the country and people who live here. We have people who really care and are really passionate about their communities, about their neighbors, about doing good things for those around them. So it’s a privilege to be a part of that, to witness it is what I think energizes this staff the entire day.
I enjoy every opportunity I have to go out and visit with our branches, to spend time visiting with people in the communities, with our members finding out how things are going for them, what they’re struggling with in their lives, not just in a financial services perspective, but are there other ways we can structure things to make their lives better and help them have more opportunity and achieve whatever their goals and dreams are.
We have numerous things that make us a great community and a great region. Because of that spread out distance we encounter we are much more connected to our neighbors and to those around us. We are much more reliant and dependent on the people that we see every day. So we get to have the privilege to be very attuned to the needs of this area and doing everything that we can to serve the people who live here.“


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Spock's Beard — Snow
(Metal Blade 3984-14406-2, 2002, 2CD)
by Jon Davis, Published 2003-02-01
Okay, so no one is going to accuse Spock’s Beard of laziness. This latest effort, apparently their last with the line-up that has lasted since their second album, presents nearly two hours of music following the story of a character called Snow, an albino cult leader (with psychic powers, I think). This is longer than most Hollywood movies, and is quite a lot to absorb, so I’ll just skim the surface. Musically, it’s more or less like the other Spock’s Beard I’ve heard, a sophisticated prog-pop hybrid with many varying sections and a high standard of musicianship. Vocals are strong throughout, and often feature complicated harmonies and countermelodies. The music ranges from gutsy rock with tight arrangements (“Second Overture” is my favorite of these) to Mellotron-heavy anthems (“Wind at My Back”), and all the players get their times to shine. But it really is the group as a whole, rather than any individuals, that makes the music work, as the arrangements feature many nice touches like Mellotron flute behind acoustic guitars and lush vocal harmonies. I don’t know if Snow will earn the Beard any new fans, but it is a strong work, impressive with its sheer size and consistency. Still, as much as I enjoy it, the whole thing is a bit long to take in, and might be even better at a shorter length.
SoundQuest Fest 2021 – SoundQuest Fest, first experienced as a live festival in Tucson Arizona in 2010 was created by ambient music pioneer Steve Roach. This 2021 event will unite a worldwide gathering of artists and audience members together for a 3-day online event unique in the realm of ambient music. From March 26-28th a continuous flow of streamed performances, audio-video wonder worlds and deep immersion zones will burn bright on Roach’s YouTube channel. » Read more
Chick Corea RIP – The sad news has reached us that Chick Corea has Returned to Forever, so to speak. The innovative keyboardist and composer died on February 9 at the age of 79. With a career that spanned from the 60s until shortly before his death, Corea touched many listeners with the incredible variety of music he produced in his lifetime. » Read more
Asia Minor Third Album on the Way – On January 29, AMS records will be releasing the long-awaited third album by classic Turkish-French band Asia Minor. Released last year in Japan, this will be the widespread debut of Points of Libration. The album features original members Setrak Bakirel (vocals, guitar) and Eril Tekeli (flute, guitar). » Read more
Harold Budd RIP – Harold Budd, one of pre-eminent American composers of avant-garde and minimalism, has died of complications from the coronavirus. Budd came to prominence in the 70s, championed by Brian Eno on his Obscure Records label, with music that blended academic minimalism with electric jazz and electronic music. Much of Budd's best known work was done in collaboration with other artists, including Eno, Daniel Lanois, Robin Guthrie, Andy Partridge, John Foxx, Jah Wobble, and many others. » Read more

domain: artistic


text:
LEROY MASON, 69
Published: Wednesday, March 12, 2003 at 2:45 a.m.
Last Modified: Wednesday, March 12, 2003 at 12:00 a.m.
WAHNETA -- Mr. Leroy Mason of Wahneta died of heart failure Tuesday (March 11, 2003) at Bartow Memorial Hospital. He was 69.
Born in Kennett, Mo., on June 24, 1933, he came to Wahneta from Missouri in 1969. He was a farmer. He belonged to New Life Assembly in Wahneta.
Mr. Mason is survived by his wife, Ruth Mason; sons, Dennis Mason, Dundee, Randy Mason, Winter Haven, Jimmie Mason, Alturas, William Mason, Wahneta; daughters, Patricia Moreen, Wahneta, Carol Powell, Auburndale, Mary Cape, Winter Haven, Charlotte Bermea, North Carolina; brothers, Elbert Mason, Irondale, Mo., Billy Mason, Advance, Mo., T.J. Mason, Lake Wales; sisters, Carol Lawrence, Bernie, Mo., Barbara Montgomery, Dexter, Mo., Vada Eaton, Sikeston, Mo.; 18 grandchildren; eight great-grandchildren.
Visitation will be from 6 to 8 p.m. Thursday at Ott-Laughlin Funeral Home, Winter Haven. Services will be at 2 p.m. Friday at New Life Assembly, Wahneta.
Reader comments posted to this article may be published in our print edition. All rights reserved. This copyrighted material may not be re-published without permission. Links are encouraged.
Comments are currently unavailable on this article


Sunday, April 15, 2012
N. Korea Shows Off New Missile During Parade
As part of celebrations marking the centennial of the state's founder Kim Il-sung Pyeongyang showed off nearly 900 units of weaponry Sunday, the largest number of weaponry showcased in North Korea's history.
They included a new type of long-range missile, which some experts say could be placed on par with the intercontinental ballistic missile, or ICBM.
South Korean and U.S. military sources say the newly unveiled missile is over 18-meters long, and has a range of up to six-thousand kilometers, giving it a capacity similar to that of an ICBM.
However, some analysts stress that it COULD be intended to be used as an ICBM, but is hard to tell at this point.
What does North Korea's possession of ICBM imply for US and its allies?
It means that NK has the capability to fire missiles US soil, possibly California.
And if the missiles were loaded with nuclear weapons, that could mean trouble for US.
Last week's failed rocket launch is good news for US and its allies because it implies that NK doesn't have the technology to launch WMDs into US. However, this is just a temporary relief.
The launch implies that NK has the intention to develop ICBMs. And these intentions are sufficient to impose sanctions on NK and deter its efforts.
Labels: North Korea


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review by Alec Clayton
|Derek Mesford as Flap and Ana Bury-Quinn as |
Emma, photo by Dennis K Photography
Run, drive, crawl to Tacoma Little Theatre to see Terms of Endearment—unless you’re not vaccinated; if you’re not vaccinated and masked, you can’t get in, and kudos to TLT for that.
I’m guessing that many of you are familiar with the story. The stage play is adapted by Dan Gordon from the novel by the great Larry McMurtry and the screenplay for the 1983 movie by James L. Brooks.
It is inevitable that the principal characters: Stephanie Leeper as Aurora, Ana Bury-Quinn as Emma, Scott C. Brown as Garett, and Derek Mesford as Flap Horton, will be compared with the screen actors in those roles: Shirley McClain, Debra Winger, Jack Nicholson and Jeff Daniels. I kid you not, these actors are every bit as stunning in their roles as were their movie counterparts.
|Stephanie Leeper as Aurora and Scott C. Brown|
as Garrett, photo by Dennis K Photography
Women who have had a difficult love-hate relationship with her mother will relate to the bantering between Aurora and Emma, and anyone who has ever enjoyed a loveable rake such as the old astronaut Garrett, who is the over the hill astronaut, and a drunken seducer of young women will be wonderfully surprised at the rocky relationship between Aurora and Garrett.
Bury-Quinn plays Emma so naturally it seems she appears to be not acting at all but simply is Emma. In the opening scenes, Leeper seems not so natural as Aurora, but soon what seems to be strained acting is actually the visible proof of Aurora’s eccentricity. And then there’s infuriating and loveable Brown. Outside of Jack Nicholson there are few actors anywhere whose stage or screen presence is so dynamic and unforgettable. It is notable that Brown was equally outstanding as Randle McMurphy, another character played by Nicholson, in the 2008 performance of One Flew Over the Cuckoo’s Nest at Lakewood Playhouse.
Director Blake R. York said in a program note that the script called for moving a lot of furniture around on stage and that he and Kathy Pingle, who was originally slated to direct, agreed that moving furniture would detract from the real crux of the play—relationships between people. The static set by York and his wife, Jen, works perfectly. The projected images on picture frames and windows adds just the right touch without being obtrusive.
Terms of Endearment flows easily from comedy to tearjerker. Bring handkerchiefs.
Terms of Endearment
7:30 p.m. Friday-Saturday, 2 p.m., Sunday through Sept. 26
Tacoma Little Theatre, 210 N. I Street, Tacoma
$27 Adults - $25 Students/Seniors/Military - $20 Children 12 & Under


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SPIDER-MAN 4 delay leads to release shifts for THOR and PIRATES 4
Now that Spider-Man 4 is officially delayed, with DHD even reporting that the VFX team involved was instructed to look for other work, two major releases have shifted their 2011 releases to fill the void.
Thor, one of Marvel’s in-house production, will slip into Spidey’s place, coming out on May 6, 2011, instead of May 20. And taking Thor‘s spot? Captain Jack Sparrow. In the wake Spider-Man 4‘s delay, Disney has given May 20, 2011, as the official release date of the fourth Pirates movie, Pirates of the Caribbean: On Stranger Tides. (via THR)
Even if Spider-Man 4 works out all of its script problems, there are not many places for Spider-Man 4 to move. Maybe June 10, 2011, a week ahead of The Green Lantern‘s scheduled release? The holiday season is even more packed than summer 2011, so that might not be an option either. Of course, if The Hobbit has to shift out of its scheduled December 2011 release, we could have a very Spidey Christmas.


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Single-use downstream applications including, chromatography, filtration, and purification create difficult environments for supplier components due to the presence of chemicals across a wide pH range. Aseptic connectors are crucial components to these processes to maintain efficiency and sterility.
For this reason, CPC is introducing a connector utilizing chemically stable and robust materials.
This presentation will cover the:
- Challenges these applications present to single-use components
- Engineering decisions made to select the new product and materials
- Benefits to the manufacturing process by using CPC connectors.
Presenter: Michael Francis is the Product Manager for the AseptiQuik connector technology at CPC.

domain: chemistry


The Casco Point ice skating rink will see some upgrades this winter.
The upgrades will come through a partnership between the city of Orono and the Orono Youth Hockey Association that the Orono City Council approved at its meeting on Monday, Sept. 13.
The partnership calls for the city to install lights at a cost of $5,000 to the outside ice rink while the hockey association will provide hockey boards to cover half of the rink at Casco Point. The project would include installation of four light poles and lights with necessary lighting controls to ensure lights only operate during designated times along with necessary electrical feeds and circuits.
Maintenance of the rink will be shared, with the city flooding the rink at the beginning of the season and as needed during the season. The city would also remove any snow from the rink after winter storms.
“I think it’s great. I think that park is under=utilized and this just opens the door for more people to use it,” council member Victoria Seals said. “The only thing I would question would be the lights. Are we sure the neighbors are OK with us putting lights there?”
City administrator Adam Edwards responded by saying there are not many houses in the area of that park, but that they would be putting the lights lower to not be much of a distraction and also that a timer would be put on the lights so they would turn off automatically at a certain each night.
“We always wanted to make our parks as much usable for all the different type things we can do in Orono,” Mayor Dennis Walsh said. “This has always been there, so if we can make this even better and more usable.”
Council member Matt Johnson asked about a warming house and if that was possible in the future. Edwards said if this partnership works that they can discuss options for a warming hous,e whether it be a temporary structure or a permanent structure, down the line.
“I think its great. We are the state of hockey,” council member Richard Crosby II said. “This is a gimme. I think this is terrific”
In other council business, the council went into closed session with city attorney Soren Mattick to discuss the status of seven lawsuits involving the city. Barbara E. Burwell in her capacity as Trustee of the Barbara E. Burwell Revocable Trust, Plaintiffs, v. City of Orono; Jay Nygard and Kendall Nygard v. City of Orono; James Hillegass v. City of Orono; Jay Nygard v. City of Orono, Minnesota Department of Human Rights; Kendall Nygard v. City of Orono, Minnesota Department of Human Rights; Barbara Schmidt vs. Orono; and Bonnett v. City of Orono (unfiled).
In the agenda packet, the city stated ‘The Council has balanced this decision to close the meeting against the purposes of the open meeting law, and the Council has determined that there is a demonstrated need for absolute confidentiality. The Council will be considering settlement negotiation status and strategy and must do so confidentially.’


text:
- Few Benefits That CBD Gummies Can Offer to You
- Heart Disease and CBD Oil – Benefits and Research to Maintain Cardiovascular Health
- Work from Home and CBD – A Must Try Combination in This Rainy Season!
- CBD After Low Impact Workout – Why to Use This Miraculous Supplement
- Introduction To Different CBD Extracts – Full Spectrum, Broad Spectrum and CBD Isolate
- Top 25 Best CBD Oil Tincture Brands
- How Can CBD Oil or Other CBD Products Kill Cancer Cells?
After her initial research, McGrath began two medical trials, one ondogs with epilepsyand one other on canine with osteoarthritis. Early outcomes are encouraging, she says, however notes that the outcomes usually are not but final or printed. Despite this lack of official steering, pet owners are more and more in search of out CBD for his or her furry members of the family. If, however, you want steering from a veterinarian about CBD on your pet, you may have to start out the conversation. That’s because vets have been overlooked of most state legal guidelines regarding cannabis, so they can talk about CBD provided that purchasers broach the subject. CBD vape pen silver CBD is thought to have many therapeutic properties, but unlike cannabis’s different main compound THC , it does not get customers high. And Powers, who can also be vice chairman of the American Veterinary Medical Association’s Council on Biologic and Therapeutic Agents, credit CBD for easing his canine’s misery.
CBD, or cannabidiol, is a naturally-occurring phytocannabinoid, or chemical compound, discovered within the hashish plant. The CBD Oil for Dogs is derived from pure, GMO-free hemp grown in Colorado. All third-party testing results are available to view on the Honest Paws website. Prices vary from $zero.16 to $0.32 per mg, making the oil middle-of-the-street when it comes to price-point. Most clients obtain their shipment within two to 6 enterprise days of placing their order.
Few Benefits That CBD Gummies Can Offer to You
Several canine homeowners administer the CBD oil in to their dogs food, however it take longer for the CBD to go to work. Chart For Suggested UseThe chart recommends dosages primarily cbd vape oil og kush justcbd based on your canine’s weight. Depending on the mg’s of CBD your bottle contains you will want to find that and your dogs weight on the chart.
CBDistillery’s full-spectrum CBD pet oil tinctures are specially designed for your pets. Made with cold-pressed hemp seed oil, your pets can now take pleasure in the identical benefits of CBD as you. that each one products are examined and the outcomes can be found for anybody to confirm.
Heart Disease and CBD Oil – Benefits and Research to Maintain Cardiovascular Health
CBD oil stays sorely beneath-researched and extra analysis is being conducted on the benefits of CBD for canines in addition to humans. Pure Hemp Botanicals CBD Oils contains solely the finest-high quality cannabidiol made from organically grown industrial hemp and is derived from a full spectrum tincture. Pure Hemp Botanicals was based on the principles cbd gummies vegan of transparency, high quality, sustainability, compassion, and charity. Their products and manufacturing processes have been created with the welfare of the planet and their prospects in thoughts. They offer 3 different dosage bottles of their Pet CBD tinctures, crafted with love and care for your house pets on up to livestock.
CBD products can help men, women and #Pets to stay cool— JustCBD (@JustCbd) February 17, 2021
Consumers need to be aware that all CBD oil for dogs and cats is not created equal.
Discover 2021’s 5 Best CBD Pet Treats in the article below
My job is to shed light on issues affecting people’s health, safety, and properly-being. I’ve dug deep into problems such as harmful doctors, deadly youngsters’s merchandise, and contamination in our meals supply. “Numbers are really helpful, but cbd pet tincture chicken cannabis is a very individualized medication,” Andre says. She advises that pet mother and father “start low and go gradual” when determining a dosage. She also suggests that utilizing tinctures rather than a chew treat could make it simpler to scale dosages up or down.
Work from Home and CBD – A Must Try Combination in This Rainy Season!
Their Receptra Pet tincture is specially formulated to be more easily digested by animals. Each product can be bought in three different sizes, they usually include a 30-day cash-again guarantee.
- a managed examine observing the results of two completely different doses of CBD (2 and eight mg/kg) on dogs with osteoarthritis.
- They delight themselves on the fact their products are vegan, cruelty free, and non-GMO.
- CBD is mostly thought of protected, however as it is not efficient to everybody due to each particular person having a special physique sort, it may trigger opposed reactions in some instances.
- Place drops directly within the mouth- watch out not to press the drop dispenser against the animal’s tongue or the facet of his or her mouth.
- This product is not supposed to diagnose, treat, cure or prevent any illness.
The hemp oil was first ready to serve the aim of selling nicely-being and well being. I give my son 600 mg twice a day for his severe anxiety and sleep problems. Though cbd just isn’t a cure it has helped him sufficient that I actually have taken him off meds. Another optimistic thing about their site is that they’ve a FAQ section. It’s an enormous help for anyone who’s simply starting out with CBD oil and CBD merchandise. With how good the model’s CBD oil is, it’s disappointing that they solely have a really small range of CBD merchandise.
CBD After Low Impact Workout – Why to Use This Miraculous Supplement
Three concentrations can be found – 240mg, 725mg, and 1450mg – all of which can be found for less than $0.15 per mg. All orders in the U.S. qualify for free, expedited transport, and most prospects receive their oil in three business days or much less.
Our CBD is always non-GMO and sourced from our personal Colorado farms with natural farming practices. We assure no pesticide exposure together with the purest components that your pet deserves. Each ingredient is particularly designed to improve mobility, support joints, reduce pain, and battle inflammation. As a result, CBD can relieve your pup’s ache and inflammation, allowing him to do the things he loves and giving you the peace of mind that he’s dwelling his finest life. Charlotte’s Web is likely one of the most simply identifiable CBD manufacturers and has built a status for its excessive-CBD hashish strain.
Introduction To Different CBD Extracts – Full Spectrum, Broad Spectrum and CBD Isolate
CBD oil drops embody a dropper so that the best dose could be measured in your Furry Family Member. CBDFX PET CBD for dogs comes at concentrations of one hundred fifty per mg for small breeds of puppies and cats, 300 per mg for medium breed canines and cats, and 600 per mg for giant giant breed canines. Honest Paws makes use of a full-spectrum oil, their product consists of hint amounts of useful terpenes that work along side CBD and a number of the different cbd isolate minor cannabinoids found in full-spectrum oils. Taking care of our pets in the absolute best way is likely one of the high priorities for all animal lovers. However, discovering meals that gives them the proper nutrient steadiness can sometimes appear extremely tough. Several cannabinoids could be extracted from the cannabis plant and this is one of The forty to four Cannabidiol that won’t drug your pets.
The best CBD topicals, including everything from creams and lotions to gels and roll-ons support wellness in specific areas to help manage discomfort. They can be your best friends post-exercise or after a long day!— JustCBD (@JustCbd) February 17, 2021
Check out all our CBD Topicals
This model’s oil has a golden yellow colour which is the usual for oils extracted through the CO2 method. CBDPure selected one of cbd infusionz the reliable and respected labs in the country to check for the quality of their CBD merchandise and that’s SC Labs.
In addition to the exams, the corporate additionally confidently shows the outcomes on their website. This is typically how hemp oils scent like if they haven’t been added to different liquids. CBDPure’s products have a golden yellow colour which is generally the usual result of CO2 extraction. If you’re just starting out or when you want something to relieve gentle headaches, persistent pain, nausea, and inflammation, you can begin with its 100mg hemp oil.
Small breeds typically experience side effects, so it’s especially necessary to get the dosage proper. It is likely one of the greater than one hundred chemical compounds found within the plant. However, in contrast to tetrahydrocannabinol , the ingredient that gives the “high” feeling related to marijuana, CBD doesn’t have any psychoactive results. Like in people, CBD oil has an anti-inflammatory effect in canine, making it perfect for treating pain and arthritis.
CBD oil ought to be given regularly sufficient that your dog experiences some alleviation of the symptoms they’re battling. Typically, it’s beneficial that canines dealing receive a dose each eight hours. If you see no change after a half-hour, you can attempt giving them another really helpful dosage. Canna Care comes in two sizes and is specially made for canine that need a higher dose of efficiency delivered straight to the issue space. Made primarily with Virgin Ucuuba Butter, Virgin Andiroba Oil, Grapeseed Oil, and Lemon peel oil, all of the ingredients in the cream additionally meet Pet Releaf’s excessive requirements for their components. You should buy CBD oil in a one hundred-mg bottle only, so it’ll up to you to calculate how many drops are right for the burden of your animal.
"If it doesn't challenge you, it doesn't change you. CBD vape cartridge honey "— JustCBD (@JustCbd) February 15, 2021
– Fred DeVito
Have you set your goals for the week yet
#JustCBD #mondaymotivationhttps://t.co/QZgC6qTwQ3 pic.twitter.com/e7mUSBDAUu
The Company takes its popularity as an industry leader seriously and has instituted finest practices by way of high satisfaction and transparency. This oil is extracted utilizing CO2, a way that preserves the full spectrum of cannabinoids, does not use heat or solvent and has additionally been examined in third-celebration laboratories.
How Can CBD Oil or Other CBD Products Kill Cancer Cells?
Those looking for ache aid might be glad to listen to that this product can act as a ache reliever. It additionally lets you eliminate anxiety and stress while promoting a constructive mood and feeling relaxed. Arguably an important good thing about Full Spectrum CBD Oil is that it could deliver pain aid. That consists of ache caused by osteoarthritis and different types of this situation, in addition to other kinds of pain.
Ksenia Sobchak – About the Author
Ksenia Sobchak enjoys blogging on fashion, style, lifestyle, love and CBD areas. Prior to becoming a blogger, Ksenia worked for a renowned fashion brand. Ksenia is a contributing author to leading fashion, lifestyle and CBD magazines and blogs. You can bump into Ksenia at her favourite cafe in South Kensington where she has written most blogs. When she is not blogging, Ksenia enjoys shopping (particularly at Harrods!), exploring the hidden gems of London, photography, jogging, yoga, fashion (she is starting up her very own swimwear brand very soon!) and traveling. Ksenia is a staunch advocate of CBD and its benefits to people. Ksenia is also on the panel of CBD reviewers at CBD Life Mag and Chill Hempire. Her favourite form of CBD are CBD gummies and CBD tinctures. Ksenia is a regular contributor at leading fashion, lifestyle as well as CBD magazines and blogs.
Interesting Facts About Ksenia Sobchak
Favourite Drink: Rose flavoured bubble tea
Favourite Movie: Trainspotting (the first one)
Interesting fact: I am a part time fashion and swimwear model
Where are we likely to find you on a Friday night: Probably enjoying a peach flavoured shisha at Beauchamp place in Knightsbridge

domain: health


text:
Anti-CD3 & CD28 antibobdy nanoparticles
|Cat No.||Size||Price||Add Cart|
Anti-CD3 & CD28 antibobdy nanoparticles are ideal to be used to activate human T cells. The nanoparticles are conjugated with both of anti-human CD3 antibody and anti-human CD28 antibody. They act as antigen-presenting cells and activate resting T cells from peripheral blood mononuclear cells (PBMCs) as well as purified T cells.
Anti-CD3 & CD28 antibody nanoparticles are provided in phosphate buffered saline (PBS) with 0.01% sodium azide. 1 ml of suspansion solution is enough for expansion of 4X107 human T cells. Store at 4°C up to 6 months.
Additional material required
Human T cell activation/expansion
For optimal results from the nanoparticles, it is recommended that the nanoparticles are washed prior to addition to samples.
1. Vortex MagVig nanoparticles for 10-20 seconds.
2. Transfer the desired volume of nanoparticles to a tube. Add equal volume of Buffer, and vortex to mix.
3. Separate the nanoparticles from the solution by placing the magnet on the side of the tube for 2-5 min and remove the supernatant carefully (with magnet still on the side). Note: A clear precipitate containing dark brown colored nanoparticles should become visible on the side of the microcentrifuge tube.
4. Remove the tube from the magnet and resuspend the washed nanoparticles in the same volume of Culture Medium as the initial volume taken to wash.
Activation of Human T Cells
1. Add 25 ul of nanoparticles to 1 ml cells (1X106/ml).
2. Incubate in a humidified CO2 incubator at 37°C, according to your specific requirements.
3. Harvest the activated T cells and use directly for further analysis.
4. For flow cytometry applications, remove nanoparticles prior to staining. Place the tube on a magnet for 2-5 min to separate the nanoparticles from the solution. Transfer the supernatant containing the cells to a new tube.
Expansion of Human T Cells
1. Add 25 ul of SI-nanoparticles to 1 ml cells (1X106/ml).
2. Add 30 U/ml rIL-2.
3. Incubate in a humidified CO2 incubator at 37°C, according to your specific requirements.
4. Check cells daily. Add rIL-2 every other day. When the cell density exceeds 2.5 X 106 cells/ml or medium turns yellow, split cultures to a density of 0.5-1 X 106 cells/ml in culture medium containing 30U/ml rIL-2.
5. Count cells on day 5, 7, 9 and 11.
6. For human pan-T cells, recommend to culture cells for 11 days.
Table 1. Guild for the use of bead volumes.
|Seeding T cell numbers||8X104||1X106||50X106|
|bead volume||2 ul||25 ul||1.25 ml|
|rIL-2||30 U/ml||30 U/ml||30 U/ml|
|seeding medium volume||100-200 ul||1-2 ml||50-100 ml|

domain: medical


text:
A rare type of ganglion cell in mammalian retina is directly photosensitive. These novel retinal photoreceptors express the photopigment melanopsin. They send axons directly to the suprachiasmatic nucleus (SCN), intergeniculate leaflet (IGL), and olivary pretectal nucleus (OPN), thereby contributing to photic synchronization of circadian rhythms and the pupillary light reflex. Here, we sought to characterize more fully the projections of these cells to the brain. By targeting tau-lacZ to the melanopsin gene locus in mice, ganglion cells that would normally express melanopsin were induced to express, instead, the marker enzyme beta-galactosidase. Their axons were visualized by X-gal histochemistry or anti-beta-galactosidase immunofluorescence. Established targets were confirmed, including the SCN, IGL, OPN, ventral division of the lateral geniculate nucleus (LGv), and preoptic area, but the overall projections were more widespread than previously recognized. Targets included the lateral nucleus, peri-supraoptic nucleus, and subparaventricular zone of the hypothalamus, medial amygdala, margin of the lateral habenula, posterior limitans nucleus, superior colliculus, and periaqueductal gray. There were also weak projections to the margins of the dorsal lateral geniculate nucleus. Co-staining with the cholera toxin B subunit to label all retinal afferents showed that melanopsin ganglion cells provide most of the retinal input to the SCN, IGL, and lateral habenula and much of that to the OPN, but that other ganglion cells do contribute at least some retinal input to these targets. Staining patterns after monocular enucleation revealed that the projections of these cells are overwhelmingly crossed except for the projection to the SCN, which is bilaterally symmetrical.
(c) 2006 Wiley-Liss, Inc.


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- This event has passed.
Elton John @ Soldier Field
August 5 @ 8:00 am - 5:00 pm
The North American stadium run will kick off on July 15, 2022, and will be making a stop in Chicago at Soldier Field Friday, August 5th, 2022!
On-sale begin Wednesday, June 30th.
“As a thank you, he’s giving the fans exactly what they could want in a final hurrah: some of his all-time biggest hits played with his classic gusto and draped in glitz.” – Billboard
“Elton John’s set on this tour is a beautifully curated trip through his golden era, with enough deep cuts to keep the musicians and veteran fans on their toes, yet so packed with hits that he’d reeled off ‘Bennie and the Jets,’ ‘Tiny Dancer,’ ‘I Guess That’s Why They Call It the Blues,’ ‘Border Song’ and ‘Philadelphia Freedom’ in the first half hour — and he’d barely scraped the surface.” – Variety
It is with great excitement that Elton John announces the final dates for his award-winning Elton John Farewell Yellow Brick Road: The Final Tour in North America and Europe. Elton will conclude his world-famous tour at major stadiums which will kick off on May 27, 2022, in Frankfurt. The Final Tour will make stops in Europe in major cities such as Milan, Liverpool, and Paris. Elton will then travel across the pond to take his final bow in North America, playing in major cities including Vancouver, Toronto, East Rutherford, Chicago, and more. The North American stadium run will kick off at Citizens Bank Park in Philadelphia on July 15, 2022, before concluding with back-to-back performances at Dodger Stadium in Los Angeles on November 19 and 20, 2022 — commemorating his iconic performance at the historic venue in October 1975. 2023 kicks off with two shows in Auckland New Zealand on January 27 and 28, followed by soon-to-be-announced dates in Australia before the epic 4-year tour formally concludes there later that year. Please visit www.EltonJohn.com
Announcing the final dates of his tour on social media, Elton John said, “Hello, all you wonderful fans out there. I’m coming to you today with an announcement I’ve been working towards for, well, all my life: the shows that I announce today will be my final tour dates ever in North America and Europe. I’m going to go out in the biggest possible way, performing at my very best, with the most spectacular production I’ve ever had, playing in places that have meant so much to me throughout my career. Whether it’s next summer in Frankfurt or at the legendary Dodger Stadium for the grand finale in the United States, I can’t wait to see you all on the road one last time. This has been an incredible tour so far, full of the most amazing highs, and I look forward to making more wonderful memories with you at these final shows. To all my friends down under, We’ll be seeing you too. Thank you and I look forward to seeing you in your town.”


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The Columbia College women’s soccer team upset the NAIA’s fifth-ranked team, Mobile, 1-0 in overtime at R. Marvin Owens Stadium on Thursday.
Three minutes into overtime, Laurie Frew drilled a free kick into the lower-right corner of the net from about 35 yards out. It was the second goal in two games for Frew, a Rock Bridge graduate. Flavia Rosado posted the shutout in goal for the Cougars (1-0-1).
Westminster 3, McMurry 2: David Immanuel scored twice and Drake Trease added a goal for the Blue Jays in their season opener.


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As Shared By Yemi Alade On Instagram…
Yemi Alade or Woman of Steel as she would loved to be called stepped out on a new style as she rocked her new sexy made in Nigeria fashion wears to her last night show performance at Abidjan.
She shared her new fashion pictures before and after the show on her Instagram page with the caption;
Wearing MADE IN NIGERIA FROM HEAD TO TOE💎💥
See more photos below

domain: celebrity


Italian food market and eatery, Eataly, will open its second store in New York City, on 11 August.
Eataly has invested $38 million, in the 5,000 m2 space, located in Tower 4 of the One World Trade Centre.
The marketplace will feature more than 10,000 high-quality food products, imported Italian goods and local seasonal specialties; sea salt, extra virgin olive oil, dried pasta, fresh truffles and aged balsamic vinegar, as well as products from small-scale farmers in the New York region.
The food emporium will also include; four casual restaurants, one upscale restaurant, six take-away counters and two bars (one coffee, one wine).
Like other Eataly markets across the world, Eataly NYC Downtown is dedicated to a unique theme, in this case-bread.
Visitors will be greeted with a prominent display of freshly-baked bread, made from wholesome, quality ingredients.
This will make New York the only city outside of Italy with two Eataly stores, bar Dubai.
© 2016 European Supermarket Magazine – your source for the latest retail news. Article by Branislav Pekic. To subscribe to ESM: The European Supermarket Magazine, click here.


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5. Jabari Zuniga, DE, NY Jets
Perhaps no position on the Jets' roster is as deep as their defensive line. Head coach Robert Saleh has emphasized defensive line depth throughout his coaching career, and his influence is all over this Jets roster.
At the moment, the Jets are legitimately six-deep at defensive end with Carl Lawson, John Franklin-Myers, Jermaine Johnson, Jacob Martin, Bryce Huff, and Vinny Curry.
Most teams don't typically carry six defensive ends on their roster, let alone more. The Jets will likely be forced to, especially with fourth-round pick Micheal Clemons also in the mix.
Where does this leave any of the other defensive ends on the Jets' roster? Well, they shouldn't exactly be feeling comfortable about their job security.
One such player is former third-round pick Jabari Zuniga. Zuniga is a former top-80 pick and arguably the worst pick of Douglas' entire tenure with the Jets.
After appearing in eight games as a rookie, barely making an impact, Zuniga was released as part of final roster cuts last season. He went unclaimed on waivers and spent most of the season on the practice squad, making three appearances and even recording his first career sack.
That sack might end up as the highlight of Zuniga's Jets career as it would take a slew of injuries for him to survive roster cuts this time. Barring any unforeseen circumstances, don't expect Zuniga to make the Jets' final roster.

domain: sports


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Ra-id Abdulla, M.D.
|Hospitals|| Presence Saint Joseph Hospital-Chicago
|Years in practice||30|
|Board certifications||Pediatric Cardiology, Pediatrics|
|Education||University of Baghdad College of Medicine, Iraq , Medical College of Georgia Affiliated Hospitals (Fellowship)|
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I began my public health career more than 20 years ago, at a time when H.I.V. was ravaging many countries around the world. The U.S. government’s strategic response to attack this cruel disease abroad was PEPFAR, an American initiative that has saved more than 25 million lives and shaped my understanding of what muscular public health policy could accomplish.
Today, as New York City’s health commissioner and a practicing doctor, I see a desperate need for similar focus and ambition here in the United States. The major health crisis we’re facing is not Covid or a single epidemic. It’s not cancer, diabetes or drug overdoses alone. It is the national emergency of worsened life expectancy in America. Combating our falling life spans could be a unifying focus of a second Biden administration, since it will take all of the government along with other sectors, working in alignment and investing at scale, to set our nation out on a path for longer, healthier lives.
Since World War II, no single period has taken more years off our collective lives than the three years from 2019 to 2021, following nearly a decade of flattening. While Covid was a factor, it was far from the only one. Countries of similar economic strength suffered less drastic drops in life span, and they have mostly regained the ground they lost during Covid. Because of chronic diseases like diabetes and heart disease — along with drug overdoses, suicide, violence — the United States has not. Some groups, such as Black Americans, have suffered even larger declines. Those without bachelor degrees have lost more years than their college-educated peers.
To reverse these trends, America should commit resources to a coordinated industrial policy for health. Industrial policy calls for government action and private markets to function together to achieve a shared goal in the face of a complex challenge. When the country needed transcontinental railroads in the 19th century, we built them through coordinated action led by the federal government. When developing countries needed H.I.V. medications, we created a mechanism for companies to produce them cheaply while building the health systems to deliver them to patients in need. When the world needed a Covid-19 vaccine, we combined government-funded research with private initiative and an enormous distribution campaign.
What would it look like, then, if we devoted ourselves to solving the problem of our decreased life spans?
First, we would have to break our addiction to clinical care designed to react to new ailments, and spend more on preventing health issues in the first place. The United States spends lavishly on clinical care, doling out $4.5 trillion — more than 17 percent of our gross domestic product — in 2022 alone. Prevention accounts for less than 3 percent of our overall health spending. That ratio buys us some of the poorest health outcomes and shortest life expectancies in the developed world.
We should greatly increase spending on prevention in two main ways. The first is to directly increase funding for public health departments to expand their core work in areas like health education, infectious disease surveillance, chronic disease screening, mental health services and doula programs to address maternal and child health — activities that have proven impacts on preventing disease and long-term health issues. The creation of a national public-private fund could draw in investments from industry leaders to match expanded government spending and send the money directly to community-based organizations that run many local and state government public health programs.
The second part is to redirect hospital and health care funding for preventive health care and for social services critical to long-term health. We’ve seen successful recent examples in how so-called Centers for Medicare and Medicaid Services waivers have enabled the use of clinical funding for nutrition programs in places like North Carolina, and in California where waivers were used to fund housing services for people facing homelessness. The Biden administration has approved eight Medicaid waivers, including one recently approved for New York. This number, along with increased waiver authority for social needs in Medicare and incentives for employer plans, should be sharply expanded in a second Biden term.
To achieve an industrial policy for health, we also would need to make it fundamentally easier and cheaper to make healthy choices, and harder and more costly to make unhealthy ones, for individuals and for industries. That means seriously considering actions like imposing excise taxes on sugary drinks and ultra-processed foods; banning menthol cigarettes, as proposed by the Food and Drug Administration, and establishing minimum unit pricing for alcohol. When implemented even in the face of tricky politics, these and similar actions have been associated with positive outcomes. We should reduce added salt and sugar in our food system through a combination of voluntary commitments, F.D.A. rules around labeling and further incentivizing fresh food purchasing for SNAP- and WIC-eligible families. We also need more incentives for healthy behavior, like consumer savings on insurance plans for joining a gym or purchasing fresh produce at the grocery store.
A rethinking of the way we plan and pay for health would require policymakers to ask different questions. If new housing were being planned, officials wouldn’t simply be expected to ask how many families could live there, how much would it cost and whether this area was zoned correctly. They would also have to answer: Would this make my constituents sicker or healthier? Would it lead to longer or shorter lives? And could this influence how many units to build, where to build them and how to set rents and further advance affordability? To answer those questions, we could embrace a new metric that projects the impact of any given initiative on mortality and life span — a life span return on investment, or L.R.O.I.
Government spending could be formally aligned with L.R.O.I. projections, and private-sector incentives could be aligned around the same framework. For instance, in tackling diabetes, policymakers could compare the L.R.O.I. of taxing sugar and fast foods versus subsidizing the manufacturing and cost of new drugs like Ozempic. They might do both, or neither — but at least they would be making an informed choice based on concrete impact on life and death.
The idea of incorporating health directly into policy decisions across the board is not new: From 2012 to 2016, eight states and the District of Columbia passed “Health in All Policies” laws, intended to orient the apparatus of government toward well-being. Though well intended, these initiatives lacked the funding, metrics and understanding needed to move the needle on population health outcomes.
Those shortcomings, and the stark life span drop we saw in our own data, informed the November 2023 start of HealthyNYC, the Adams administration’s plan to achieve healthier, longer lives in New York City. In 2020 alone, life expectancy here dropped an astonishing 4.6 years, to 78 — the worst decline in the country. We have set an ambitious goal of reaching 83 years by 2030, which would be among the highest in the country. We’ll do this by tackling the leading causes of overall and premature death — from heart disease and overdoses to suicides and cancer.
At the same time, we are creating models so leaders across the city government can begin to interrogate spending decisions based on a version of L.R.O.I. The New York City Council even passed a law to codify HealthyNYC into planning, obliging regular progress reports and a reassessment of life expectancy goals every five years, ensuring that this transcends electoral cycles and the priorities of any single administration — something that will be essential for any federal effort to be successful.
A national project along these same lines is beyond what most believe our deeply divided political system can accomplish. That’s exactly why we need to try. There is an opportunity for President Biden to announce a national commitment to reclaiming American life expectancy as a centerpiece of his campaign and a second term. The president has previously framed the election in November as a choice between two competing visions for the future: a backward-looking politics of grievance, division and demonization, and an inspirational, aspirational and hopeful vision for the future of America.
An industrial policy for health could offer hope and direction for the future, and a salve for polarization. It’s hard to imagine a more unifying proposition than “we all deserve to live longer, healthier lives — and ensuring that we do should be our government’s No. 1 priority.”


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Tuesday May 26, 2020
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Pentagon confirms death of 1 U.S. soldier in Afghanistan
Published : 17 Sep 2019, 23:47
The U.S. Defense Department confirmed on Tuesday that a U.S. soldier died in Afghanistan on Monday.
The soldier was killed by small arms fire when his unit was engaged in combat operations in Wardak Province of Afghanistan, said the Pentagon in a statement, adding the incident is currently under investigation.
The United States maintains some 14,000 troops in Afghanistan, which largely provide training missions to local Afghan forces while also conducting counterterrorism operations against terror groups.


A WOMAN was hit by a lorry in Battle town centre.
The 75-year-old, from Battle, was struck by the white lorry as she crossed the High Street at 11.20am on Wednesday April 30.
The woman suffered a number of broken teeth, bruising to her face and a swollen ankle.
PC Nigel Dunn said: “I would like to speak to anyone who was in High Street at the time of the collision and saw or heard what happened. Please contact me on 101, quoting serial 475 of 30/4, with any information at all that you have.”


Managing domestic energy assets for the benefit of households and networks
Homes of the future will increasingly have multiple energy assets such as electric vehicles (EVs), , solar PV and battery storage systems. The MADE (Multi Asset Demand Execution) project is all about understanding how to manage these multiple energy assets in a way that is both beneficial to the homeowner and the energy networks.
*a hybrid heating system usually combines a gas boiler or an oil boiler with an electrically driven heat pump – together often referred to as a ‘hybrid heat pump’.
MADE concept overview – a home with multiple energy assets controlled in an optimised manner in order to benefit both the homeowners and the electricity networks and system.
The MADE project objectives include
- Investigating the ability to manage multiple energy assets. This will also include focussing on the switch between gas and electric load to provide fuel arbitrage and highly flexible demand response services.
- Demonstrate the potential consumer, network, carbon and energy system benefits of large-scale deployment of in-home energy assets with an aggregated demand response control system.
- Gain insights into the means of balancing the interests of the consumer, supplier, and network operators when seeking to derive value from the demand side flexibility.
The MADE project clearly demonstrates the importance of understanding end user customers as a core component of the future energy system. Delta-ee’s expertise of understanding customers and their role in the energy transition will help the MADE project develop new insight by:
- providing a firm evidence base around future uptake scenarios of the different low carbon technologies;
- carrying out primary customer research to evaluate consumer trust in new technology that is taking greater levels of EV charging, heating system control;
- modelling the impact the uptake of these low carbon technologies has on the low voltage grid; and
- analysing new and existing business models across Europe and the rest of the world with the view to start developing some potential customer propositions in this space
Interesting high-level findings emerging from the project to date:
- Fairly strong growth in the sales of all low carbon heating technologies is expected in the medium to long-term. Sales of electric vehicles are going to multiply rapidly in the late 2020’s. The deployment of specific low carbon technologies will however be heavily influenced by government subsidies and policies.
- There are large variations in customer behaviour. There isn’t a one size fits all solution. Optimisation of customer assets will need to consider different use patterns, etc. However, any optimisation will need to address the current concerns homeowners have around third-party control of EV charging and heating.
- There are multiple value streams that can be realised to help drive the economics of adopting multiple low carbon technologies. Realising these value streams in most cases will require market reforms. There are many, mostly untested, customer propositions that could be used to deliver the MADE concept. For example, some of these could leverage concepts such as heat as a service.
- Our modelling shows that the uptake of MADE technologies could place significant strains on the low voltage network. Based on the current structure of market price signals, optimisation of energy demand at the household level could even in some cases make matters worse at the regional or national level. This means loads need to be carefully optimised and balanced across the electricity system. This will help ensure conflicts are minimised and there are benefits to householders, the local network and the national system. Further research is required to characterise the nature of the optimisation and balancing required.
Delta-ee is excited to be part of the MADE project. This £1m+ Network Innovation Allowance Project by WPD – led by PassivSystems, is at the cutting edge of future energy system transformation. Delta-ee’s long standing expertise across the new energy transition perfectly positions us to be part of this project.
If you want to find out more, please don’t hesitate to get in touch.
MADE project partners:


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The star, real name Benjamin Ballance-Drew, has teamed up with EMI Music Publishing to launch a joint venture called Temperamental Music.
In his new role, Plan B will oversee and sign new songwriters and artists to the firm's roster.
He says, "I've always wanted to be in control of my destiny and build my own empire. This is a step closer to that...
"I feel I can help raw young musicians, planting some positive seeds in their head, help them progress."
EMI Music Publishing president Guy Moot adds, "You have to have a huge amount of respect for all that Ben he has achieved not just as an artist but as a writer in the last five years. He knows what it takes to become a successful writer, and he recognises real talent when he sees it."