Some Common Painkillers Linked to Miscarriage Risk
Study Suggests Miscarriage Risk From Using NSAIDs Like Ibuprofen or Naproxen During Pregnancy
WebMD News Archive
One limitation of the study was that it relied on NSAID prescriptions as a marker for their use.
In Canada, one popular NSAID, ibuprofen, is also available over the counter.
As a result, many women in the study who were counted as not taking NSAIDs because they didn't have a prescription might actually have been using them.
Also, getting a prescription doesn't mean that a person actually takes the drug, says Gideon Koren, MD. Koren is a pediatrician, toxicologist, and pharmacologist who directs the Motherisk Program at the Hospital for Sick Children, in Toronto. He was not involved in the study.
Researchers in Sweden, who were looking at the safety of using migraine medications during pregnancy, recently tested the reliability of prescription records by going one step further and interviewing women about their medication use.
They found that many women didn't actually take the drugs their doctors ordered for them. And Koren says that besides their prescription history, there were also important differences between the women who had miscarriages and the women who did not that likely muddied the study's results.
One difference that leapt out at him involved prescriptions for anti-nausea medications, which are sometimes prescribed for morning sickness.
About 15% of the women who didn't have miscarriages got prescriptions for anti-nausea drugs, compared to about 3% of the women who miscarried, a fivefold difference.
"Morning sickness protects against miscarriages," Koren says.
Other differences between the groups were higher rates of depression and anxiety and poverty in the miscarriage group than in the comparison group, though researchers tried to remove the influence of those things by adjusting their data to account for them.
Advice to Women
Taken together, he says he thinks pregnant women should not worry if they have taken an NSAID medication.
"Scaring pregnant women is a national sport in Canada and the United States, and I don't think this is good," Koren says. "It may lead women to not treat themselves when they need it, or even to terminate a wanted pregnancy. I think we have to be extremely careful before we make an association that has major methodological issues."
Study researchers acknowledge that more research is needed to prove that NSAIDs cause miscarriage.
But Bérard says she thinks that in this case, when there are other, perhaps safer choices of pain relievers for pregnant women to use, it's good to get the word out in case the increased risk is real.
"At least during the first trimester, women should think about safer alternatives, and I'm thinking of acetaminophen here," she says. Acetaminophen is the generic name for Tylenol and multiple other medication brands.
Bérard says women can lower their risk of inadvertent exposure by planning their pregnancies.


text: On Thursday November 22, 2018, witnesses reported seeing the victim’s white 2001 Chevrolet Impala stopped in the eastbound lane of IH 10 E, between WW White and Loop 410. There was a blue Ford F150 stopped to the right of the victim’s vehicle. The driver of the F150 fired multiple gunshots through the passenger side window of the victim’s vehicle, striking the victim in the head. The F150 then immediately fled the scene. The victim was transported to SAMMC where he died on November 24, 2018. The suspect vehicle was described as possibly a 2008 to 2010 two-tone Ford F150. The top portion of the vehicle was dark blue with a sliver or lighter color on the bottom half.

domain: news


Engine oil is an integral part of strong engine performance. Oil ensures your engine's metal parts are properly lubricated. It provides a much-needed cooling that vehicles can't properly function without. It's no secret that regular oil changes are mandatory to prolong the lifespan of your car. However, when life happens, it can be easy to forget you until your car starts to display some symptoms. Below are three signs; it's time to schedule an oil change.
Dirty Engine Oil
Engine oil can pick up outside debris over time, reducing its quality. This makes it essential to check your engine oil at least once a month. Engine oil should be honey or amber-colored. The fluid should be clean and free of any pollutants. If the oil is dark and littered with dirt, it's time for an oil change.
Unusual Engine Noises
Gritty engine oil will not effectively lubricate the engine, causing it to work harder than normal. Oil creates a barrier between the engine's metal parts, and without it may cause rumbling, grinding, and knocking sounds.
Oil SmellIf your windows are closed and notice an oil smell inside your car cabin, it may be time for an inspection. If you haven't recently fueled up, a gas smell can indicate a gas leak. Leaks can limit the amount of oil flowing through the engine and cause overheating or a blown head gasket in severe cases. If you suspect there's a leak, bring your car to an expert.
Oil change service in Des Moines, IA
Don't wait until you notice signs of damage to schedule an oil change. Motor oil keeps your engine running smoothly and improves gas mileage. Engine oil flushes dirt, debris, and engine pollutants within the system. If the oil has degraded, sludge can accumulate and decrease efficiency. Richman Automotive & Towing is a Napa Auto Care repair shop specializing in all vehicle makes and models. Our ASE certified technicians have been servicing the Des Moines community for over 25 years. To schedule an oil change, click here to schedule an appointment.
Or give us a call at 515-461-8780 Mon – Fri 7:30 AM – 5:00 PM.


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On May 17, keynote speaker Dianne Wilkerson addressed the first graduating class of the Black Economic Justice Institute’s Marcus Hall Civic Engagement Academy and pointed to the students to as an example of why Black lives matter.
“The major movers, shakers and organizers of the activity that really blew up after we saw that video of the public execution of George Floyd was born on the backs of young people. That’s who led this," Wilkerson said.
The recent report that details how little the City of Boston spends with businesses owned by people of color revealed that over the last six years, Black businesses have received just 0.4% of Boston's procurement/contract spending —
This reflects poorly on our city’s leadership — especially as Mayor Marty Walsh, prepares to go off to Washington as labor secretary to implement President Joe Biden’s economic plan. That's a plan that, as Biden himself said, "includes communities that have historically been left out of government procurement — Black, brown, Native American small businesses and entrepreneurs in every region of the country.”.
Brother Lo, Co-Founder of BEJI and a member of the Black Boston COVID-19 Coalition expressed dismay that the Coalition had been unable to secure a meeting with him since April to discuss the need to address COVID-19 issues in highly impacted Black and other communities of color.
As COVID-19 spreads across the City of Boston and the Commonwealth of Massachusetts, The Black Economic Justice Institute, Inc. (BEJI) and Boston Praise Radio & TV (WBPG-LP 102.9 FM) are committed to chronicling its impact on the Black community. BEJI will document stories, advocate loudly and do what is possible to keep their neighbors healthy through information and assistance. Boston’s identity is shaped by the narratives of its people, all of its people, and BEJI and Boston Praise Radio & TV’s mission is to ensure that Boston’s Black Community stories are reported and heard. Read More HERE
Massachusetts has suffered some of the highest death rates in the country from the coronavirus, partly because a wave of infections tore through nursing homes across the state early in the pandemic. About 1 in 7 Massachusetts long-term-care residents died from COVID-19, a Boston Globe Spotlight report found in September. One of those victims was Ruby M. Flint Kinney, who was living in a Mission Hill nursing home when she died from the virus in April. Her daughter, Priscilla Flint-Banks, still has unanswered questions: Why didn’t her mother’s nursing home have designated places where people who tested positive could be isolated at the start of the pandemic? Read More Here (Copyright (c) Boston Globe
BOSTON — Priscilla Flint-Banks’ buried her mother Ruby on April 29, one day after what would have been her 88th birthday. “Everybody that knew her loved her," said Flint-Banks. “We called her Mother Kinnery. Very sweet, outgoing person.” Ruby Kinney’s cause of death was COVID-19, but Flint-Banks says the staff at her mother’s nursing home in Boston never told her Ruby was sick. “Never did no one say, ‘We’re isolating her because of this or we’re putting her there because of that,’ said Flint-Banks. “I still don’t know what happened.” Flint-Bank is not alone in her grief. Watch the video and read more HERE
Nearly 26 years after a ballot initiative banned rent control statewide, voters may soon have a chance to reconsider.
Joseph Feaster, Segun Idowu, Mayor Martin Walsh, and Darryl Settles enjoy a moment during the Black Economic Council of Massachusetts annual meeting. PHOTO: JOHN WILCOX, MAYOR’S OFFICE
The Highland Park Neighborhood Council voted this week to ask the city to rename a portion of Dudley Street to Chuck Turner Way in honor of the former city councilor, who died last month.
Entrepreneurs and nonprofit workers can sign up as members of Reevx Labs by first entering their names and emails into Berkshire’s database. They can then reserve space in the 2,200-square-foot lab’s conference room, workstations or multifunction room. Source: BayStateBanner
Black residents of Boston have been hit hardest by the coronavirus outbreak, and East Boston has been the hardest-hit neighborhood, new data shows. According to data released by the city, the city had confirmed 7,910 cases in the city as of Saturday. Of the cases, 271 were fatal cases while 1,573 patients had recovered. Read More HERE
BOSTON--As Black organizers begin the second night of camping outside of historic Faneuil Hall, Mayor Marty Walsh continued to avoid meeting about setting a public hearing date related to changing the name of Faneuil Hall.
Kevin Peterson, the founder of The New Democracy Coalition, (NDC) was beginning the second day of a hunger fast, as Walsh ignored a small contingent of activists seeking to hold him to his pledge to address systemic racism, in Boston.
“Your words that you spoke two weeks ago about making Boston a national leader, concerning ending structural racism, ring hollow,” said Reverend Joseph Rocha, a member of the NDC. “We call on you, Mr. Mayor, to keep your promise.” At 12 noon on Wednesday, June 24th, in front of Faneuil Hall, Peterson and others will address the news media with a special statement.
A settlement between Massachusetts and a company accused of ignoring rules for hiring minoirty and woman subcontractors highlights how hard it is for these firms to participate in state contrcts even when they are written into the requirements. Read More HERE
Boston’s black community has been hit hard by the coronavirus, with a staggering 39% of the known COVID-19 cases in the city traced back to African Americans, health statistics show. Read more HERE
By Danny McDonald Globe Staff, Updated December 29, 2020
Massachusetts has suffered some of the highest death rates in the country from the coronavirus, partly because a wave of infections tore through nursing homes across the state early in the pandemic. About 1 in 7 Massachusetts long-term-care residents died from COVID-19, a Boston Globe Spotlight report found in September. One of those victims was Ruby M. Flint Kinney, who was living in a Mission Hill nursing home when she died from the virus in April.
Her daughter, Priscilla Flint-Banks, still has unanswered questions: Why didn’t her mother’s nursing home have designated places where people who tested positive could be isolated at the start of the pandemic? Did the home’s staff have access to enough personal protective equipment? When did her mother’s health decline? Was it gradual or sudden?
She didn’t even know her mother had the virus until after her death.
“COVID is real, it’s not a fantasy, and our people are dying rapidly every day,” said Flint-Banks, a 65-year-old community advocate from Roslindale and cofounder of the Black Economic Justice Institute.
Read More Here (Copyright (c) Boston Globe
by Amelia Mason and Miriam Wasser Source: wbur.org
Like a lot of people with loved ones in long-term care facilities, Priscilla Flint-Banks spent much of the spring worried about her 87-year-old mother, Ruby Kinney. Kinney, who had dementia, lived in the Edgar P. Benjamin Healthcare Center, a predominantly Black nursing home in Roxbury. In early April, Flint-Banks called to check on her mom and spoke to a nurse. She says she asked whether the home had any COVID-positive residents. Read More Here Copyright (c) WBUR
BEJI distributes $40,000 in grocery & pharmacy gift cards to Boston's Black families.
Audit finds 36% of nursing facilities are not adhering to states' COVID-19 guidelines. Leaders of BEJI demand further investigation and justice.
Black and Latinx advocates used Memorial Day to commemorate the people of color who have died from COVID-19 and demand a rollback of the state’s reopening plans.
Members of the social justice group Mijente organized a protest that ended on the steps of the Massachusetts State House Monday. Representatives of 50 other advocacy groups joined the crowd and urged Gov. Charlie Baker to reconsider reopening procedures.


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Compiled by Freya Ensbey.
Easter is a time that is rich in tradition, for those celebrating the religious connotations, as well as those who simply see it as a holiday to enjoy time together, while indulging in the sweeter things as a family.
A lot of us may chomp down on chocolate eggs over Easter, but little do most know where this tradition came from. Originally eating eggs was not allowed by the church during the week leading up to Easter, known as Holy Week, so any eggs laid over this time were saved and decorated to make 'Holy Week Eggs,’ and then given to children as gifts. This tradition soon turned into creating the symbolic eggs with chocolate so that the children could still enjoy eating them.
However, we believe that no one needs permission or an occasion to justify celebrating with chocolate, but we do believe the amount of time one can spend perusing pages of chocolate recipes cuts into precious indulging time, so we have done all of the hard work for you with these chocolate recipes that will make for the most decadent Easter yet.
Used as a side to any dessert or a standalone star of the show, a smooth and creamy mousse recipe is a great tool to have up your sleeve. With minimal ingredients and no need for baking, this decadent chocolate mousse from Perth restaurant, Post, melts in the mouth and has the added nutrients and good fats of avocado. The added crunchy texture of chopped pistachios on top will make this your go-to favourite raw recipe to whip up in a breeze.
This dark chocolate pudding recipe will bring on the oozy sauce, so all you have to do is add the deliciously cold ice-cream to cool things down. Made in individual ramekins, it means that there is no need for sharing this decadent dessert with anyone.
Darren Purchese has let us in on his secrets to the perfect mousse as a component in this visually stunning dessert, also sharing with us his ethos behind it; "everyone needs a great, simple chocolate mousse recipe up their sleeve. This one is quick and easy to prepare but silky smooth and decadent. It's a soft-set mousse for pouring into and serving from glasses or dishes, so just increase the gelatine if you are planning to unmould the mousse. Remember to use the best-quality chocolate that you can afford for the best results.”
Semifreddo is translated in Italian as "half cold.” Used as a base, this recipe is easily adaptable for adding in nuts, chopped pistachios work well or pieces of fruit and can be topped with any silky sauce of your choosing. It is also a fool proof way to substitute the timely practice of making an ice cream and does not require an expensive churner.
For a fuss-free dessert to whip up after a long day of activities during the Easter holidays, try George Calombaris’ chocolate fondant recipe. With a chewy top, cracking into a soft and gooey centre, this fondant is sure to warm the hearts of all the family and makes for a great way to get everyone involved in the process.
If you think that lasagne can only be savoury, think again. We have found the ultimate Italian chocolate devotee’s dessert with this chocolate lasagne, layered with crushed Oreos, choc chips, cream cheese and chocolate pudding. We suggest serving a generous portion into a bowl and topping with a scoop of good quality vanilla ice cream.
Kid Friendly Recipes
With the kids, usually on school holidays over Easter, it can be demanding to keep them entertained. Getting them involved in the kitchen is a great way to keep their hands busy, helping to create masterpieces they will be proud to show off, so we have created a list of our favourite kid friendly recipes to keep the little bunnies busy for hours.

domain: food


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...that the day I'd published my last post happened to be the 50th anniversary of Doctor Who! To commemorate this, I'm deciding to share an earlier picture I'd almost forgotten about:
This is definitely among my favorites. I'm not quite done with it, though--the "vortex" should probably be more like a textured, part-invisible cylinder (like in the 8th Doctor movie that I didn't like) rather than multiple rings made of halos, as I'd rendered. Chances are that I could achieve first this effect using a ramp/colorband (a way to fit lots of colors on an object without textures or faces).
What I should probably go for
Meanwhile, I've been preparing for finals at college (which is going moderately well), digressing on careers as an author, singer and/or global dominator, and have redesigned my blog! I'll share my Photoshop experiences later!
Finals are fast approaching for me and I notice that I've only posted one post on my college experience. So, here's the rest of the stuff I've been doing in Adobe Illustrator:
You'll have to trust me that the symbol didn't start out purple. The goal here was that I'd use the Pen Tool to "trace" the odd now-purple symbol by a man named Arnold Varga, the trick being that it had to be achieved with 25 anchors (clicks) or less.
The pen tool uses Bezier Curves, a kind of curve that makes curves that are always in a 180 degree relationship. In other words, if I click and drag and draw 3 vertical lines identical in height exactly .3 inches away from each other, they look like this:
Actually, I don't know quite how else to describe Bezier Curves mostly because I don't know how they work myself. Either way, though, you get better at it when you practice.
2. The Crash Course.
This experiments with just about everything else in Illustrator--typing text, linking it to a path and fitting it into a shape, giving text fill (color) and stroke (outline) color, breaking up the text into individual objects to make them look generally weird, making a picture out of basic shapes (the duck) and then making it into a randomizable symbol, applying gradients (rainbow) to two squares as both linear (lines) and radial (circle) and to the brush tool [stops for breath], turning a scanned image into a brush and changing its color (purple and blue), and for those of you who are still awake and conscious, taking three shapes and merging, separating and dividing them.
This project had two parts: I had to, using four squares of potentially different locations, sizes and rotations, think up 10 combinations of squares representing the words bold, increase, tension, and playful. Then I had to choose what I thought to be my best 3 pictures for each word and redraw it in Illustrator (some modification of the squares was allowed). I had a hard time with "tension" until my teacher said I should try to "make Alfred Hitchcock nervous"--and walked up to about two feet away from me and asked if it made me feel uncomfortable, and then told me to put that feeling into the picture. Unsurprisingly, I had vast improvements a few minutes after.
For this assignment, I was supposed to make a pattern--preferably one inspired by Celtic, Japanese or a few other country's designs--out of basic shapes and apply it to a few other shapes. Mine was Celtic-inspired. However, having the Bill Nye "patterns" song running through my head whenever I was working on it--at all--did NOT help.
5. The Assessment.
No need for complicated explanation here; the assessment just takes everything I learned and puts it to the test.
I'll post my PhotoShop and InDesign assignments in future posts!

domain: artistic


Former Southampton striker Graziano Pelle would save the club from the peril they are currently engulfed in.
Leeds United would surely be safe from the drop if they had a prime Mark Viduka to partner Patrick Bamford.
It has been one year since a Wolves striker scored in the Premier League, something which would perhaps not be an issue for Julen Lopetegui if Kevin Doyle was still at Molineux.
Leeds United are a side struggling for goals, and would love a prime Jermaine Beckford back at the club now.
Everton are lacking any attacking impetus from the midfield, and injecting a prime Mikel Arteta into their current side would surely provide that.
Leeds United would likely swap the disappointing Brenden Aaronson for the mercurial Pablo Hernandez in a heartbeat.
Former Sunderland wing-back Bali Mumba would have been perfect for Tony Mowbray.
Rangers could do with having club legend Stefan Klos back between the Ibrox posts.
Sunderland would love Kevin Phillips back now, as the marksman would surely fire them back to the Premier League.
A prime Wayne Rooney would be perfect for Frank Lampard's current Everton, who are lacking a classic striker such as him.
Xabi Alonso would star under Jurgen Klopp in his Liverpool side, and would vastly improve the midfield.
Rangers would love to have former defender David Weir back at the club in order to solve the current defensive crisis.
Jonas Gutierrez, who is regarded as a Newcastle United legend, would be perfect as a squad player for their current side.
Carlos Cuellar would shine under Giovanni van Bronckhorst in this Rangers team as they battle a defensive crisis.
Sol Campbell would be the missing piece of the jigsaw for Arsenal under Mikel Arteta.


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Before I start, let me just state for the record that I actually do adore my pets. Really, I do. They are part of my family and I am so glad that we adopted them (Humane Society fur babies for this family, all the way).
But there are times when the fur babies drive me just a little cuckoo-for-cocoa-puffs. Take this instant. The cat is taking a few laps across my desk and laptop in her morning affection ritual. Oh, and the dog finally got done alternating between sniffing the cat’s butt and laying his 70 pound head on my forearm. Who knew that the pets would suffer from sibling rivalry so badly? The dog loves the cat. The cat tolerates the dog. But both get twitchy if the other is getting attention from me. Oh, and both have to be in the bathroom if I’m in there, pestering me to pet them. Talk about a captive audience.
But the worst part of being a pet owner to these particular little critters is that one is bulimic and the other is anorexic. I know, rather unlikely that one family would be struck by polar eating disorders, but sometimes strange things happen.
My cat is a puker. She often realizes that she can see some dish bottom between the kibbles in her bowl so then she scarfs up as much kibble as she can stuff into her little self. Inevitably this process makes her thirsty – all the hard work bingeing like that entails. About 3 minutes after her post-binge foray to the water bowl and I can hear her yacking up her food. It isn’t even slightly non-kibble shaped. Like, you could chew your food little missy! It wouldn’t bug me so much if she would recycle it, but no, she has standards.
Speaking of standards, my dog has them as well. He won’t eat her barely moistened cat kibbles either. Of course, he also won’t eat anything off the floor. Oh, periodically he’ll have a weak moment and eat a slice of fallen hot dog, but he refuses to eat stuff off the kitchen floor. Seeing as this was one of the primary reasons Mrs. Claus agreed to getting the mangy little mutt for Christmas a few years back, it would be nice if he’d act more dog-like and actually hoover-up the kid crumbs. But no. If you put a tater tot on a plate and put the plate on the floor, he’ll eat the tater tot. Roll the tater tot off your plate and table while expanding your tableware skills, oh hell no, that dog has higher standards than that.
For pete’s sake, these are animals who lick their own backsides. You’d think having spent time at the critter orphanage would have made them more appreciative for dining opportunities that present themselves, but no.
So while I love my little fur babies, there are times when I think rather mean thoughts. And by the looks of my kitty, that should be in about two minutes. I don’t know who needs a 12-step program more – them or me.

domain: pet


* This section is intended for developers who want to customize to an extended product based on the WIZ750SR hardware or software.
The full firmware source code of the WIZ750SR product is available at the GitHub repository, and it uses the Apache License 2.0, anyone can freely modify(called 'customize') and distribute it without restriction. These documents describe how to restore the product through the firmware update if the product does not work during the user's customization.
If the user wants to customize the application area only and keep using the configuration tool functions of the WIZ750SR product(Device Search / Setting / Update and Etc.), Users can use the 'Network Firmware Update' function of the configuration tool to update the modified application to the WIZ750SR and check if it works well. At this time, if there is a problem in the operation of the customized application firmware and the search function does not work, AppBoot mode can be used for search and recovery the device.
If you want to customize the entire firmware while maintaining the WIZ750SR's hardware, you can update the full binary directly to the code flash memory by using the W7500x ISP Tool(WIZISP) of the W7500 / W7500P chip using a serial port. The WIZ750SR-EVB is equipped with a switch(BOOT) for accessing the MCU boot mode. It can perform ISP (In-System Programming) operation through Debug UART (Micro USB connector) located on the EVB side.


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WSU create a one credit course to allow international students to stay in U.S.
0 have signed. Let’s get to 5,000!
The new decision by the US Student and Exchange Visitor Program (SEVP) requiring international students who are taking an all-online course-load for fall semester to leave the United States or risk arrest and deportation, we urge Washington State University and President Kirk Schulz to create a one-credit credit-no-credit class with one meeting a semester as well as excused absences in all semesters, until this outrageous policy is reversed. Doing this would allow international students, already burdened by the stress of studying in a foreign country and the perpetual uncertainty about their visa status due to the pandemic, to stay in the US without fear of deportation. WSU has a large community of international students ranging from student athletes to TA’s. Forcing them to leave due to this policy is not the way cougs look out for each other. We must stand with our international students they way they stand with us by choosing to bring their talents to WSU!!
The decision by SEVP and Immigration and Customs Enforcement (ICE) is outrageous, discriminatory, inhumane, and an attack on our international student friends. It is ridiculous to force international students to leave the country simply because they are taking only online classes. Additionally, many international students have no way of returning home because of travel restrictions. For example, Chinese international students cannot return home because the Chinese government has restricted international flights into China because of the pandemic. International students who stay in the US without taking in-person classes risk being arrested by ICE and being locked up in ICE detention centers. ICE detention centers are known to have horrific conditions, be carrying out human rights abuses, and to be hotbeds of COVID-19 transmission.
We strongly urge WSU admin and President Schulz to respond to this petition and do everything within their power to protect the WSU international student community from the inhumane organization that is ICE, and ensure that they can stay in the US without fear of arrest and deportation.
THIS PETITION WAS INSPIRED BY AND DESCRIPTION USED BY ALEXANDER TSAI FOR THE USE OF UW LINK TO UW PETITION:
Today: Sahana is counting on you
Sahana Umesh needs your help with “Washington State University: WSU create a one credit course to allow international students to stay in U.S.”. Join Sahana and 4,325 supporters today.


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domain: economics


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Please go to https://tennesseetreetoppers.org to join or renew your membership, to get pilot information, event information, news and so on.
- If you are a current member, you should have received a welcome email with instructions and login information.
- If you are a pilot and would like to join, please click on the Join Now menu button at the top of the page.
The new website is a work in progress. We hope to have more informative information and photos to share, so please check back from time to time.
Before your first flight at any of our sites, you must first be familiar with our Pilot Information, Flight Regulatory Program requirements, and Site Information pages available on this website and you should request a briefing. Site briefings are required for first flights by Novice (H2,P2) pilots at Henson Gap and (H3,P3) pilots at Whitwell. Site Briefing Contact Form

domain: hobby


Brandenburg instead of Bali: Workations and bleisure travel are expanding in Germany too
’New Work in tourism – what’s in it in Germany?’, was the title of a discussion round on the Hybrid Stage at ITB Berlin, organised by TMB Tourismus-Marketing Brandenburg GmbH (www.reiseland-brandenburg.de) and moderated by Dr. Andreas Zimmer, departmental head, Cluster Management, at TMB. On Tuesday afternoon the focus was on terminology, best practices and prospects. According to the journalist and chief editor Sylvie Konzack (https://bleisuretraveller.com/), this emerging market still has great potential, with only 15 per cent of the market currently exploited.
Professor Peter Neumann of IU Internationale Hochschule (https://www.iu.de/hochschule/lehrende/neumann-peter/) explained that there was a trend towards new forms of work under the heading ’new work’, along with a trend towards new forms of travel. The combination of business and leisure, or work and vacations, came under the heading ’bleisure travel’. “Workations are basically a kind of working holiday where people work from a different location, if possible in a relaxing environment that offers inspiration“, Neumann said.
According to Neumann there was a substantial market. “19 per cent of the working population and 30 per cent of companies know about workations“, Neumann said. According to a Euromonitor survey, bleisure travel was among the fastest-growing travel markets worldwide. New hybrid forms of travel under the collective term ’blended travel’, which above all attracted the younger generation, were increasingly becoming the focus of tourism managers. In Germany, the states of Rhineland-Palatinate and Thuringia were trailblazers in specialist bleisure travel, whereas Brandenburg was especially innovative in promoting workations, he said.
Vanessa Thielemann, founder of the BrALI Team Retreats & Offsites (https://www.brali.org) project, detailed her concept. At a time when many people work remotely, she wants to give teams the opportunity to meet in an environment that offers inspiration, and to that end is working closely with the Zukunftsorte Brandenburg (https://zukunftsorte.land/) network. She believes it is important that her services are not seen as coming from outer space, but as a way to reach out to the local region and population.
Nico Gramenz, who is currently setting up a Europe-wide network of workation providers under the heading Project Bay (https://project-bay.de/) is focusing on partnerships with hotels – and has already secured a large number in Mecklenburg-West Pomerania. “We want to establish a Europe-wide network of 50 partners. At present we already have 32“, said Gramenz, who sees his service not only as a business model but also contributing to the development of local regions.


Recap last night’s Big Brother episode.
Shania Twain on the Playlist.
Homemade Cherry Ice Cream…. YUM!
One of the most important words in the English language
Today’s Sunrise shot from the West Palm Beach Waterfront Park overlooking Palm Beach Island
- Shania Twain’s Las Vegas Show Proves She’s ‘Still The One!’ (reellifewithjane.com)
- Jimmy Buffett Buys Third Home in Palm Beach Florida for $1.3 Million (prweb.com)
- 10 Biggest Summer Jams From 1998 (buzzfeed.com)
- Shania Twain to release first new album in 11 years (independent.ie)


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The Last Station
Did you see this movie?
The Last Station is a 2009 English-language German biographical drama film written and directed by Michael Hoffman, and based on Jay Parini's 1990 biographical novel of the same name, which chronicled the final months of Leo Tolstoy's life. The film stars Christopher Plummer as Tolstoy and Helen Mirren as his wife Sofya Tolstaya. The film is about the battle between Sofya and his disciple Vladimir Chertkov for his legacy and the copyright of his works. The film premiered at the 2009 Telluride Film Festival.
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Sometimes, there are several reasons of someone to have an engagement party firstly before throwing a real wedding procession. One of which is long distance relationship. Most women prefer to keep her boyfriend engagement before letting him go away for long distance relationship. If he really loves her, a man must want to propose her to have an engagement vows. At this moment, ring is important to have. It has the same function as wedding rings as a love vow symbol. Solitaire engagement ring may be a good choice for it. Let’s find the best design idea of that ring below.
In general, the typical characteristic of solitaire engagement ring design is quite difficult to find. We usually feel that some rings in solitaire design concept just have similar look. There is no any special touch or detail which usually can distinguish the solitaire ring with another ring design. One thing which can be found as a typical characteristic of solitaire engagement band is its round band. The round band here is a part of ring which is formed in round shape. This round band is a thing that allows everyone can wear a ring on her or his finger. In this case, the solitaire ring has very slim band. And it is quite different from standard rings which typically have thicker bands. This condition is not a serious problem on the solitaire ring. The slim band precisely makes it more unique and meaningful than usual ring. Another characteristic of the solitaire ring for engagement and wedding is an artistic carved detail. This detail is commonly applied on the external side of band as decoration.
In the other side, the application of artistic detail on the band is replaced by diamond grains attachment. The last but not least characteristic of the solitaire engagement ring is its six-prong part. This part can be found on the top of the ring. The prongs are arranged in hexagonal or round pattern to hold a big diamond as embellishment of ring. With this six-prong, it doesn’t matter if the diamond cut will be formed in any concepts including Sun Burst, Princess Cut, Marquise cut and many more.

domain: design


text:
How To Make Paper Kunai Knife
People Who Liked This Video Also Liked
Did this video help you?
Johari Stewart: No tape
Khoyrul Islam: you suckkkkkkkkkkkkkerrrrrrr
Jose Castro: -________-
Mika Hatsuki: Stuck at 1:39
messfree21: wow your vid suks a monkey can do that and get more viws then you
Bradley Chrestensen: thanks for that video cuz my bro showed me it and i made like four
breckbshide: This is horrible I didn't understand
xxreshiramloverxx: u know you can take a video 2 u know
sultanb97: sad because ur calculator can only take pictures
JustPaperGuns100: @KillcamsVsFeeds lol
esw420: i get lost at 1:06
BennyBoyAnimationsxD: what the****
Lazar Mirić: azianxx@ yeah it happened to me, im dead
icniVaD DaVinci: retard
bgludobmw: merci pour le tuto
nigahiga493: thanks idot
MasterM M: i like
rikitiki55: awesome thanx
Joe Mama: I did it perfectly.....
Seth House: it is not good.
xX15thprestigexX: @shay6844 u suck u pickle any idiot can do this so shut up
beerskids: Azianxx don't scare the crap out of the little ones
barfbagdude8: this is confusing
xDPhuntard: A Kunai is a throwing knife, if I translate the title it will be "how to
make paper throwing knife knife"
Paintballerz15: thats retarded i cant figure out 44 through 50
Daan Pronk: 3 words freak this video
Kyle Brendan: YOULL DIE AND GO TO HELL BUT SAITIN WONT LET YOU IN AND YOULL ROT WHILE
FELLING SORRY 4 YOUR SELF AT THE SAME TIME!!!!!!!!!!
mirotzu99: are they throwable?
Darth Aussie: @mariomanil IF YOU POST STUFF LIKE THIS TONIGHT YOU WILL DYE BYE AN UBER
Kyle Brendan: FUC U!
goppy0: ok, since you had a camera WHY NOT RECORD YOU DOING THE FREAKING THING!
MrJohnnys619: Pfff you copied Returacan but hiskunai is better!
Johhny Gat: no dont kill me clown!!!
Riley Sperbeck: niiicccee
zomkillto5: a effin noob
sam murphy: why would chuck norris want to make a paper ninja knife????
Shayla Lyons: you suck
BladeEdgeDRunner: THIS IS SO AWESOME THANK YOU!!! =d
ilovesmurfette45: can anyone help?
Gavin Raabe: you suck big fury pickle
TheMnMbu5ters: music is good wat song is it?
BladeEdgeDRunner: @BladeEdgeDRunner OOPS I MEANT =D
MrGabtoon: VERY GOOD!!!!
justhere55: i would love to see a moving video step by step not just picture, no
thepokemonmaster3100: wtf man
popcornds: errrrm dont understand
how to make paper kunai knife
out of 5


text: Every now and then good days come along just when you need them. Today was one of those days. For some reason I had been wondering if everything was really worth it. If all the trouble and stress we go through is worth it. That thought kept playing in my head. This evening I went with Hannah to the 100 acres where we walked around for a while. We found a clearing with a bench that had this view. We sat there and talked for the longest time about all sorts of things. Little things, big things, memories and feelings. And as I sat there thinking about so many things, talking to Hannah and experiencing the woods around me I suddenly realized that, yes, everything is worth it. Moments like this, spent with someone like her, are what make everything worth it. I won’t ever forget sitting on that bench with her, talking about everything and anything, as the sun slowly climbed down and the lake became a mirror and I realized that life is worth living.

domain: literature


The Mirrycle Jellibell is a fun way to let pedestrians and other riders know you're coming! The Jellibell has a see-through top so you can see the gears spin as it rings. It's weatherproof, too! - Dome Diameter: 48mm (1.9") - Fits handlebars with 21mm – 22.5mm (.83" – .89") outside diameters
The sleek and ultralight BM-45 bar end mirror easily attaches to drop and flat bars, making it perfect for all bikes. Constructed of aluminum and glass for durability and nearly distortion-free viewing, the BM-45 represents the height of handlebar mirror construction and design. Minimal projection from the bar means never having to sacrifice clean aesthetics for critical rear-view safety again.
Mirrycle's Incredibell Adjustabell 2 uses a fully adjustable handlebar clamp that fits any size bars. Its perfect size and pivoting ringer let you mount it just about anywhere on your handlebars, and allows you to ring it with your thumb or finger. The Adjustabell 2 can even be mounted to a ski pole or walking stick. One flick and it rings loud and clear for all to hear. - Weight: 26g (.9oz) - Dome Diameter: 34mm (1.34") - Fits handlebars with 15mm – 39mm (.59"- 1.54") outside diameters
This lightweight, small mirror from Blackburn adheres directly to your helmet for a great rearview to make all your rides safer. It can be removed in seconds, too.
Mirrycle's Incredibell Road Bell is little, light and convenient. Its small size and pivoting ringer let you mount it on either right or left Shimano STI shifter/brake lever. A flick of your finger and the Ding! Ding! rings clear for all to hear. - Dome Diameter: 28mm. Domes are solid brass – black or chrome plated - Will fit either left or right lever - Weight: 26g
New patented thumb trigger improves strike force and volume. - 36mm alloy dome - Adjustable position - Ring sound: DING!
Mirrycle's Incredibell Brass Duet Colors makes a traditional ding-dong dual-tone ring with the beautiful resonance of solid brass. Its compact size lets you mount it just about anywhere on your handlebars. And, the bell's vibrant colors add a splash of personality to any bike. - Dome Diameter: 33mm (1.3") - Fits handlebars with 21.7mm – 24mm (.86" – .95") outside diameters
Modern, unique low-profile design made from high-polished brass, mounted to a machined aluminum base. The brass striker politely and effectively alerts others with a sharp, definitively loud ring. A simple O-ring attaches the bell to a variety of tube sizes and shapes, elegantly complementing all styles of bikes.
Little, Light, & Loud! Our Original Incredibell has been redesigned for quick and easy mounting on nearly any handlebar. The pivoting dinger can be rotated to any position.
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Lezyne's Classic Brass Bell features a compact, classic shape constructed from modern, high-quality materials. The bell itself is made from high-polished brass, mounted to a machined aluminum base. The small brass striker provides a sharp, impressively loud ring when flicked, warning others of your presence. A simple O-ring attaches the bell to a variety of tube sizes and shapes while integrating cleanly with any bicycle. Weights - Small: 21g - Medium: 28g
- Can be used on arm, leg, backpack, or bike - 4-LED design for 360 visibility - Built-in passive reflective strips - Water resistant - Mode: Flashing Off - CR2032 Battery (included) - 75 hour run time - Sold individually
Fits around the ankle or the upper arm for increased visibility - Hook-and-loop fastening - Durable fabric construction
- Elasticated Velcro strap provides many mounting possibilities - Diffused LED light provides 360 visibility - Built in passive reflective strips - Water-resistant - 2 Modes: Off Steady, On Flashing - CR2032 Battery (Included) - 75 Hour run time
- Screw-on expending plug designed to stay tightly in place - Easily folds away to facilitate transportation and storage - Tool-free adjustment - Compatible with most handlebars
- Durable all steel trigger bell - Floral feminine pattern - Bell diameter: 80mm
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Lovely Ding-Dong ring sound - Perfect complement to a classy cruiser - 80mm wide by 50mm deep - Sturdy twin-screw clamp mount
- Easy installation and angle adjustment - 3-inch diameter - Compatible with most handlebars
- Easy installation and adjustment - Compatible with 17.8 - 19mm inner diameter bars
Mirrycle's Incredibell Spring Bell offers a traditional Asian-style bell that integrates into the top cap of the headset to free up valuable handlebar space. The bell can be mounted any way you choose on the headset, and the dinger is adjustable as well. A flick of your finger and the Spring Bell rings loud and clear for all to hear. - Dome Diameter: 45mm (1.8") - Weight: Black and Silver: 26g (.9oz), Brass: 57g (2oz)
Moose Horn for kids' bikes.
Bike bells generally look & sound a bit ugly. But why? What if they looked sexy & sounded like an angel playing a glockenspiel? A bell with a beautiful tone, but also a remarkable style and choice of materials that embodies a rider’s personality. The Small Oi fits 22.2mm diameter bars, e.g Mountain bike (near the grips), BMX & some old commuters. Please make sure you know where on your handlebar you are going to place the bell (grips or stem) before you order as you may need a different sized bell. THE LOOK Where bells usually protrude up or out, Oi doesn’t. It appears to hover around the handle bars. In one sense, it's discreet. But in another, it stands out as unrecognisable. THE SOUND Knog tested numerous prototypes to get the perfect pitch, length of ring, and volume. With a spring-loaded actuator built into the mount, the strike of the bell is firm and fast, allowing a good balance of volume and longevity of sound. It actually has several pitch tones - one core and several supportive higher tones to ensure it's unmistakable and safely heard. To compare it to a musical instrument, where some bells play a single note, Oi plays a chord. THE MOUNT What about the handle-bar cables? No worries. Built into the shape of the mount is a cable management system. So not only does it allow the bell to look and sound uninterrupted, it also helps gather your cables (for the organiser inside us all). What about getting it on the bar. Again, not a thing. Just pop it over, give the hex screw a quick tighten up, and you're good to go. No sliding down the handlebars, no dismantling and re-assembling.
Let the world know you're there and add some whimsy to your bike with Evo's Fun Horn. It's fully compatible with Dura-Ace, Super Record, and Red groups.
Made of solid brass, this bell’s melodic sound is full and old-fashioned. Big and brassy, the perfect accompaniment for cruisers, comfort, and town bikes. - Dome Diameter: 51mm (2") - Fits handlebars with 21.7mm – 24mm (.86" – .95") outside diameters
$31.99 - $33.99
Keep valuable handlebar space free with Mirrycle's Incredibell Striker Bell. It integrates into the top cap of the headset, can be mounted any way you choose, and features an adjustable dinger as well. A flick of your finger and the Striker Bell rings loud and clear for all to hear. - Dome Diameter: 45mm (1") - Weight: Black and silver: 36g (1.3oz); Brass: 68g (2.4oz)
The Emoji Bell emits a classic dring-dring ring sound. - Express yourself with these cheeky bells - 52mm wide by 28mm deep - Sturdy twin-screw clamp mount
$17.99 - $19.99
• Classic Dring-Dring ring sound • Express yourself with these cheeky bells • 52 mm wide by 28 mm deep • Sturdy twin-screw clamp mount • Your friends will love it
Specifically designed to quickly and easily fit on all mountain bike handlebars, Blackburn's Mountain Mirror features a premium-quality convex mirror for a wide, clear view. Plus, the mirror's vibration-damping arm helps prevent blurring.
The Basil Portland Bell Brass makes an instant impression. The timeless silver finish ensures an easy complement to almost any bicycle frame, while the bell itself is easily mounted to your frame, with no complex installation to worry about. When rung, this bell emits an audible sound that’s loud enough to alert other cyclists, motorists and pedestrians of your presence.
$19.99 - $22.99
A new angle on bells. Low and lean looking; very techy too. - Fits handlebars with 22.7mm – 26mm (.0.89" – 1.02") outside diameters
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Science Nursery Decor Set of 6 in Coral Color
Add a dash of scientific wonder to your nursery with this set of 6 coral-colored wall art prints! Featuring a microscope, DNA strand, and other adorable illustrations, it's the perfect way to inspire curiosity, exploration, and a love of learning!
This poster set includes 6 science art posters.MATERIAL & INKS
- Standard Frame sized print, easy to find your own favorite frame!
- High quality colors
- Museum quality Heavyweight ultra smooth matte paper
- Frame is not included


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Why can’t this just be a Catwoman-Umberto roadtrip, or – more Hellhound! more deathtraps! more pages lost to explaining plot elements for the Batman story going on!
The virus storyline Batman is partaking in continues elsewhere, but this is it for Catwoman’s involvement/not involvement (how would Batman like it if another super butted into ‘his’ story arc about the return of his archnemesis??). And the last we’ll see of Hellhound and his many many knives.
Catwoman and her crew and the Bat gang belong to DC Comics, and Escher Girls is the creation of the awesome Ami Angelwings. And the Escher Girls bingo card belongs to the Escher Girls fan who created it.


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Camp Hale (Colo.) -- Archival resources
Found in 20 Collections and/or Records:
Aaron J. Schenck Papers
Content includes correspondence from Schenck to family members while at Camp Hale, Colorado; Camp Swift, Texas; and Italy. Also includes illustrations done by Schenck throughout, as well as collected souvenir postcards, newspaper clippings, photographs, Army documents, and other memorabilia. Schenck organized the collection contents chronologically into scrapbooks; this order has been preserved.
Alfred Ricken Papers
Includes telegram and correspondence from War Department notifying Emma Bischke Ricken of her husband's death and letter from Ricken to Albert Schaeffler. Photo album includes photos of Camp Hale, rock climbing, unidentified soldiers undergoing training, Cooper Hill, Leadville, inside tavern or bar, and rock climbing at Seneca, West Virginia.
Anthony N. Smith Papers
Collection consists of approximately 200 letters from Smith to his parents from 1943-1946, including newspaper clippings, photographs and postcards. A letter from February 11, 1946 included a burned letter that had been in an airmail plane crash. Notes regarding content description of 185 letters were provided by Barbara Mason (Smith) Selby.
Ballard G.Busby Papers
Includes 13 large photographs (copies) showing Camp Hale (Colorado) and Italy during World War II, one small photo of Pvt Haslam, correspondence, and a Bronze Star Medal citation (July 5, 1945).
Charles W. Thweatt Papers
This collection contains materials from Charles W. Thweatt detailed his experiences and responsibilities in the 10th Mountain Division of the United States Army during World War II. Content includes military records, photographic prints, correspondence, and newspaper clippings collected during the war and later on in his life.
Donald Albert Petersen Papers
Collection contains memoir: My Father, Soldier (2016). Memoir based on information collected by son, Michael P. Peterson.
Edward J. Redle Papers
Includes Redle photograph, rosters, notes, articles dealing with Company K, 85th Infantry Regiment (copies)
Evelyn Supply Papers
This collection contains memorabilia from Supply’s time in the United States Army at Camp Hale, Colorado. Content includes her United States Army documents, correspondence, photographic prints, newspaper clippings, and booklets.
James Gilronan Papers
John and Walter Kasabuski Papers
Includes photocopies of news articles, and clippings, photocopies of photographs and letters about John and Walter Kasabuski.
John H. Pierpont oral history
Discusses Pierpont at Camp Hale in December of 1942. Recalls drills and practices in 20 degrees below zero weather. Went to Kiska in July of 1943. Served in Italy taking part in the assault on Mt. Belvedere. Talks about men being killed by minefields and grenades that Germans lobbed when they were pretending to surrender. Recalls partisans who killed Germans at night and his furlough in Rome. Concludes with post war information about the 10th Mountain Division Association.
Martin Bockelmann Papers
Includes news clippings and notes for an article from The Independent Family Living, "A Veterans story: Martin Bockelmann, World War II Veteran."
Morton Lewis Papers
Photographs from Camp Hale and Italy, as well as information booklet from Camp Hale
Ray Jarnstrom Papers
Ray Jarnstrom's Memoir "Music at Camp Hale" 190th Army Ground Force Band.
Reamer A. Bolz Papers
Color slides from Italy in Spring 1945, Italian currency, a religious card from the Vail Reunion of 1983, and correspondence between Hugh W. Evans, Reamer A. Bolz, and Denver Public Library staff member Barbara Walton.
Robert Donald Hansen Papers
Collection contains military records, booklets and insignia, correspondence to and from family members, and photographs from training and time spent in Italy.
Robert John Barth Papers
Collection contains military documents, photographs, ephemera, and a scrapbook relating to Barth's service with the 10th Mountain Division in WWII.
Stanley E. Leszko Jr. Papers
The collection consists mainly of photographs taken by Leszko, but also includes slides, diary pages, separation papers, and newsletters. The photographs have been kept in the original subject groupings arranged by Leszko.
Tibor L. Mikes Papers
Includes a photocopy of a manuscript entitled Notes; includes details of Mikes escape from Hungry prior to World War II.
William I. Neder Papers
Summary of daily diary entries, correspondence (photocopies), photographs (JPEG files), short stories (photocopies), and oral history (MP4)

domain: library


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These are the comments others left on tracks by theHumps
Yee Hah - once again - love it Wayne
Long live the humps
Anything with a bit of country blues does it every time man took about 3 sec for the big cheesy grin on my face to appear brilliant and as only you can do :)
The Bard of Looperman does it again. Three listens! Can't help it but whenever I hear that bass pattern and that drum beat I just think Johnny cash. But it's perfect. Nice warm fuzz guitar. Highly entertaining listen man.
We recognize your style right away!! great mate! completely in the spirit with your fucking voice! good job Wayne!Michael
HOW, just how that guitar is sounds soooooooo firmly vintage? Damn its a superb work!
The guitar is a $90 Squire Stratocaster through a $160 amp. I adjusted the pickups a little and did a few small things to it but it's a cheap ass guitar thru a practice amp. It works for me, makes a decent sound for what it is.
Hi! it feels like we are back in the good old days! excellent, good mix.Michael
The good old days.....
Thanks Michael, kept it simple and to the point.
YOU GOT ME WITH THE TITLE THEN BLEW ME AWAY WITH YOUR BEST TRACK EVER....I KNEW THIS WAS COMIN FROM YOU....AND THATS WHY IM A FAN
Thanks man! Very simple and basic idea, only 4 tracks.
I had to fave this fucker. Being that I had an ear-to-ear grin on my face from the first chord and for the full two minutes and nine seconds. I mean, it just goes and goes and goes and then it ends, and someone like me is sitting there, sweaty from dancing in his chair, "Wow, that's a perfect song right there." There, I said it. Perfect song right there.
I like when the bass and guitar are in sync, it makes the song tight and punchy.
Thanks again Mofo!
Yeahhhh! your work is so super cool! & as I have said before your lyrics are something else!..I truly like your style, GREAT! vocals also & loving that guitar too. ROCK ON!
Thanks you so much again for your comments!
Another very cool humps hit - love it
I know its a slightly different theme but was reminded of https://youtu.be/KzEmtD3IO1Y ... Im an adult now
Glad you liked it!
I love that song, been a while since I heard it but it was played a lot on the stations I listen to back in the day.
i love this one man its not like your other stuff your voice here reminds me of alice cooper the way you sing it just the voice not the song the mixing sounds superb very good work hey thanks for that video i liked it he reminds me of the the guy that follows zoro around with the instrument sitar or zither cant remember never new of him way he works neck is like me i swich my hand in difernt directions and finger tap alot some say its show boating but when you do it in a live jam its not showboating it means your spot on comfortabal in the zone his strum hand how ever reminds me if a sitar or zither player never les thanks for the link
Alice Cooper? Now that's not one i've heard before. There's a few videos that accompany that one, hope you checked them out too! Dale was a showman, he loved all the tapping and strutting around, he was a great performer.
Dirty, gritty and great riffs. Bass guitar is my favorite in this track. Vocals Reminds me of Nick Cave. Nice work.
That Nick Cave, he's such a bad seed. ;)
Great description and context. All of my formal education is in finance, accounting, and economics. Lately I've been cramming study flashcards to "test out" of college courses so I can sit for a CPA exam (after about 60K of loan debt of course, so technically still plenty broke).
Because of this, issues such as income disparity, crypto currency bubbles, the "higher education hustle," geopolitical complexities, etc... won't leave my mind.
This song managed to sum it up in a simple concise way.
Nice work. Faved.
Probably my best teacher in HS was my economics teacher. He gave a $1000 scholarship to the student in our class who made the most money playing the stock market with an imaginary $10,000 he gave each of us. He taught us what each column meant on the stocks page, told us how the market worked and we had to make at least 3 trades a week. It was fun! He always used Levi's jeans and Jordache jeans as examples even tho he alway wore a button down shirt and slacks. We all chipped in and bought him a pair of Levis and gave them to him weeks before we graduated, he wore them proudly.
I think all education should be free thru college except for private schools. An educated society benefits all and it should burden them financially after school. I went to a state college and my debt was only about $12,000 back in the 80's. If Reagan hadn't slash the Pell Grants and govt. tuition help everyone needed it would have been much lower. Trick down economics suck when you're the one being trickled on. lol
Thanks man, just a quick, simple song!
defeniatley me, only difference i did have a lot, just fucked it for myself though and lost everything :(( so always building Wayne, but a lot older and wiser, i still let myself down at times just like most but im happy, for a long time i wasnt, i dont blame anyone other than myself, i was a handful tbf but finding a deeper love for music and likeminded people, changing how i go about day to day, important things, not worrying about things i CANT change, not judging of others, then 2 years ago, the most important becoming a grandfather 3 times now :)) stay resolute guys, only you can change your life for the better. great track and story telling Wayne, always worth clicking play when i see you up there and the music is always on point
Older and wiser, that's what we become. We all make mistakes and bad choices growing up, that's a big way to learn about life. We can't control anything in our lives and people think they can, that causes suffering as the Buddha would say. Just try not to piss for a couple days or never get sick. We can't even control our own bodies never mind anything else, lol!!
Gotta just enjoy every moment by being in the moment, not thinking about the past or the future. Mindfulness.
Thanks for swinging by!
very cool tune Wayne ... you play these dirty, punk, root music amazingly well ... I like the rumble of the drums and the sure "foundation" of the bass bomb ...hats off bro, Danke
I've been forcing myself to learn to play, record and mix the electric guitar more. So making these straight forward rock songs help a lot, especially when my electric gits aren't the best, learning to better eq them and compress them to get a fuller, quality tone.
like that chords man.song reminds me of me haha and prob a million others.I actually treated myself to 2 new toys been saving up, i have got myself a new sunburst fender bass sounds not bad but my cream de la cream is my banjo deliverence here i come :)
as always man cool tune :)
It should remind you of a million others, there's only so many chord progressions used in rock and roll, lol. Basic chord changes, a song like this doesn't need anything fancy, it's a 3 piece banging out an angry song. AC/DC/, Green Day and a thousand other 3 piece bands never used complicated arrangements or chord structures. That's is what this is.
A sunburst Fender Bass? What year? Jazz? Precision? Very Cool! I borrowed a banjo for a summer, years ago from an old bandmate, who drank himself to death, sad. He gave me a lesson book with songs in it and a floppy vinyl record to put on my turntable and follow along with the lessons. Boy, what a loud instrument. Might get one of those 6 string acoustic guitars that plays like a banjo someday. They even make a sitar version 6 string.
Thanks man! Have fun with the new toys!
Hey Wayne, been busy lately so I've been off LM for a few weeks. Getting back, one of the first things I did was search your profile to see if you had a new track. And you did not disappoint!
As usual the playing was on point and so was the old-school production. I think my favorite aspect of this one was the lyrics though. They seem to convey the simple yet kinda oblique wisdom of a bygone age. "Here's the story of just another working class person. Hear it, and take whatever you can from it."
Lots of new material always being posted.
Just a quick idea, short. Sat on this one for a month or so, wasn't sure if it was the way I wanted it, still not.
Thanks for stopping by Cam!
took a few days but i was thinking of Hank Marvin
I had to look up who that was. I do recognize the band, am familiar with it.
Would never have guessed him.
Oh man...I`m taken back to the days we were doing gtr instrumentals & what became known as surf.The guitar sounds good as does the vocal..must say I always enjoy stopping by your place when I get a bit of time Wayne...always unique and always good listening..groovy...keep on..Ed
Thanks Ed! Big fan of surf music, Dick Dale was a genius.
Thanks for the nice words!
very nice man this awsome love your guitar work here dude if you like surf music get the squire jaguar diference between jazzmaster and jaguar is a lot short scale neck for one seymour duncan pickups more switches both surf guitars with floating bridge tremlo the tones are unreal you can get one for 360. new gears for music or spash out get fender jaguar any where from 450 to 2000
Appreciate all the info!
I can't use those small guitars, I'm a big guy, long arms and big hands. A jazzmaster sounds nice but they have inherent problems at the bridge/tremolo. Playing a mando is a challenge, but it's very small.
Yee ha - great stuff as usual, warm and bright and a great sound and the cool little guitar lead at the end there is the icing on the cake.
I'm trying to make a $500 guitar sound like a $3500 guitar, he. It gets the feeling across with enough twang.
Ding Dong sing a folk song, great track again Wayne, your vocal is top notch as always great guitars lead and bass and the drums are good sit just right, great listen, Vegas CITY OF SIN most definately
I don't know how the words evolved to that line, City of Sin, but it got there and I like it. Works for me, lol!
Very cool the guitar reminds me in a mixture of roy oberson or dam i forget his name now always played with a fender it will come to me at the most inconveniant time had glasses in big goofy teeth but was ace on guitar and had his own style anyway man very cool tune as always :)
Buddy Holly, perhaps you are thinking of? Played by Gary Busy in the movie the Buddy Holly Story, great movie!
This is a real toe-tapper of a tune! you got me up & moving to this little ditty so early this morning. I have to say, I love your writing skills the lyrics are GREAT! and the music something special and your vocals simply brings this whole composition right home!...It's a home run! as far as I'm concerned & a Fave! Wonderful! Work! Kudos! :)
Thanks you again for your comments! I've been working hard on the story telling and the mixing, they're moving forward so that's good.
another great track
another humps classic
long live the humps
Glad you liked it man, thought this would appeal to your appreciation of music. :)
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Looper Time : 2022-06-30 20:53:16 | Version l-3110


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1. Regeneron COVID-19 antibody drug reduces infection risk 82% in clinical trial Full story
2. Kaiser strike expected to affect all of its Northern California outpatient pharmacies Full story
3. ISMP warns about mixing up pediatric and adult COVID-19 shots Full story
4. Dashboards alone can't improve infusion scheduling. See what made the difference for UCSF Health here.
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12. Daily COVID-19 stats, state-by-state:


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The child is very much father to the man in “Staying Vertical,” a serenely nutty cinematic pastoral that unfolds at the crossroads of life and death, freedom and responsibility, this world and the next. The film follows a screenwriter in his 30s named Leo (Damien Bonnard) into the open countryside, where he shacks up with a shepherdess, has a baby boy and soon finds himself dealing with much more than just a crippling case of writer’s block.
The movie, for its part, hardly wants for creative inspiration. You could probably imagine a much straighter (in every sense) version of this story — one that doesn’t feature quite as many growling wolves, eccentric sexual configurations and scenes of botanical electrode therapy.
But really, where would be the fun in that? The French writer-director Alain Guiraudie has a gift for burrowing his way into the human soul by way of the libido, and he works in a searching, serio-comic style that refuses to separate life’s wildest extremes into neat, narratively expedient compartments. With “Staying Vertical,” he has made the sort of movie that is often described as “not knowing what kind of film it wants to be” — a dismissal that, in this case, should instead be considered high praise.
Guiraudie came to international prominence with his 2013 Cannes Film Festival sensation “Stranger by the Lake,” a wickedly seductive thriller that observed the comings and goings at a secluded gay cruising spot with both erotic heat and chilly analytical rigor. To say that “Staying Vertical” touches on some of the same preoccupations — the great outdoors, the dark side of desire, manhood and its discontents — is at once perfectly true and faintly misleading. What it lacks in its predecessor’s exacting formal control, the new movie more than makes up for in its capacity to surprise. It could be titled “Stranger by the Minute.”
It begins simply enough. Leo finds himself traveling among the windswept wilds of southwestern France (beautifully photographed by Claire Mathon), searching for writerly inspiration and fascinated by the wolves that inhabit the region. He falls into bed with a rural woman named Marie (India Hair), who oversees a flock of sheep with her gruff dad, Jean-Louis (Raphaël Thiéry), and her two young sons. She soon gives birth to a third, an event that Guiraudie observes in unsparingly gooey detail — one of several full-frontal close-ups that, under the camera’s unflinching gaze, seem to evoke Gustave Courbet’s famous nude, “The Origin of the World.”
But Marie, the only female character of any stature, soon recedes from the picture, moving to the city with her two older sons and leaving Leo to care for their infant alone. Leo continues to drift, putting off the screenplay he’s writing and dragging his son along from scene to scene with an odd mix of affection and carelessness.
He also pays regular visits to a nearby cottage where Marcel (Christian Bouillette), a grouchy old shut-in, likes to blast Pink Floyd and yell anti-gay slurs at his houseguest, a sullen young man named Yoan (Basile Meilleurat). Their love-hate codependency seems forever on the brink of shifting into outright homoerotic desire, and it finds a strange echo in Leo’s own continually evolving relationship with Jean-Louis.
By the time Leo gets into a canoe and paddles his way downriver in search of a marsh-dwelling plant therapist (Laure Calamy), “Staying Vertical” has taken on the demented illogic and inexplicable clarity of a dream. Not the kind of dream that teems with bold, hallucinatory imagery, but rather the kind that thrives on steadily escalating degrees of narrative derangement, in which even the story’s weirdest formulations seem rooted in the banality of the everyday.
Characters disappear at random only to reemerge in strange new configurations. Traditional roles are slyly recast; people you didn’t think knew each other turn out to know each other. The movie’s circular, repetitive structure increasingly seems to mirror the cyclical nature of all existence. Sex is linked as closely to death as it is to life, l’origin du monde and la petite mort rolled into one.
Its title a sly reference to what distinguishes men from beasts, “Staying Vertical” hinges on the tension between primal instincts and socially proscribed behavior. Guiraudie isn’t just trying to decimate sexual taboos; he is also taking gently comic aim at the overly rigid roles into which people tend to lock themselves. And he has an ideal collaborator in Bonnard, the kind of actor who keeps you at a slight remove initially only to become an increasingly empathetic figure as the movie wends its way toward its strange, and strangely moving, destination.
Of all the roles a man might be called on to play in the world — father, lover, protector, provider — how does he reconcile any single one with all the others? Guiraudie’s crazily compassionate filmmaking creates a space for all manner of male fantasies and anxieties, however dark, shameful or grotesque, to assert themselves. And he does this without losing sight of those wolves prowling at the edges of the story — a reminder of the very real predators lying out there in wait, to say nothing of those lurking within.
In French with English subtitles
Playing: Laemmle’s Royal Theatre, West Los Angeles, and Laemmle’s Playhouse 7, Pasadena


ARTEMIS integrates with OpenSight
Global roll out of ARTEMIS mobile phone detection, location and communication sensor continues with FlySight collaboration. This integration can be seen at EUROPEAN ROTORS, the VTOL show and safety conference, 8-10 November 2022, Cologne, Germany.
Smith Myers announces that its ARTEMIS mobile phone identification, geolocation and communication sensor is now available as an integrated airborne search solution with FlySight Open Sight Mission Console software
The agreement combines OpenSIGHT-mc and ARTEMIS for deployment in airborne missions, where the OpenSight augmented reality engine can be used to optimise search results, reduce operator workload and lead to more rapid positive outcomes.
Designed to work with any FlySight options ARTEMIS can detect a mobile phone at extended ranges and provide unique capabilities such as mass-mapping and geofencing.
Read full article from ADS: https://www.adsadvance.co.uk/smith-myers-artemis-integrates-with-flysight-software.html
Watch the video here where the voiceover explains the integration:
The collaboration is the first between Italian-based FlySight and U.K. based Smith Myers, both already deployed with customers globally across SAR & Disaster Relief applications, across rotary and fixed wing aircraft


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The Sears store formerly on Gateway Drive has moved to Price’s Plaza at 1820 Howard Road, and store manager Patrick Deming is pleased with the new location.
During one of many trips into Fresno’s River Park area from Madera, Joe Oganesyan had an epiphany.
“Why not open a frozen yogurt place in Madera?” he thought.
It’s a simple formula that brings customers to the Valley Pistachio Country Store: keep the products local and the service friendly.
For Agriland Farming Company founder and CEO Jim Maxwell, the best way to learn the ins and outs of a successful business is to be directly involved with the profession.
Most people don’t give a second thought when eating that bowl of rocky road ice cream or taking a bite of a protein bar as to where the product comes from.
A large-scale dairy is the New York City of the agriculture world — it never sleeps.
Consumers snacking on a bag of Blue Diamond almonds or Sun-Maid raisins might be eating the very nuts and fruit grown on Wattenbarger Ranch, a property that overlooks the San Joaquin River near Avenue 7 in M


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Oqton looks quite a bit different than the last time we spoke with them.
3D Systems announced a new series of industrial 3D printers, the SLA 750.
Stratasys announced two new partners to their GrabCAD Software Partner Program.
3D Systems and Desktop Metal announced notable corporate acquisitions.
Oqton announced it has raised a massive US$40M from investors.
What happens when 3D prints change from being prototyping devices to production machines? A lot more quality control, that’s for sure.


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When you are attempting to look for a quality as well as a reliable source of peptides, we understand how tough it sometimes can be. Pharma Lab Global chose to create this educational page for the purpose of helping you make your decision a bit much easier. We believe that we are a truly various peptide shop, setting a new level of standard in the industry of peptides.
We breathe and live quality & dependability as well as professional service. Our company is to make certain that we provide 2 things for our well-regarded clients. First of all, to provide the highest quality peptides that are readily available anywhere in the world. The second thing is to offer all our clients with world class fast responsive customer care throughout the year with a smile.
We’re very positive that when you have actually decided to make your initial buy from Pharma Lab Global, you’ll never ever go to buy peptide from anywhere else again.
Everything You Need to Know About Peptides
Peptide Bond – What Is It?
A peptide bond refers to the covalent bond that gets produced by two amino acids. For the peptide bond to happen, the carboxyl group of the first amino acid will require to respond with an amino group belonging to a second amino acid. The response causes the release of a water particle.
It’s this reaction that leads to the release of the water particle that is typically called a condensation reaction. From this reaction, a peptide bond gets formed, and which is likewise called a CO-NH bond. The particle of water launched during the reaction is henceforth referred to as an amide.
Formation of a Peptide Bond
For the peptide bond to be formed, the particles coming from these amino acids will require to be angled. Their angling assists to make sure that the carboxylic group from the very first amino acid will undoubtedly get to respond with that from the 2nd amino acid. A basic illustration can be used to demonstrate how the two only amino acids get to conglomerate through a peptide formation.
It likewise takes place to be the smallest peptide (it’s just made up of two amino acids). Additionally, it’s possible to combine numerous amino acids in chains to develop a fresh set of peptides.
- Fifty or fewer amino acids are known as peptides
- Fifty to a hundred peptides are called polypeptides
- Any formation having more than a hundred amino acids is usually regarded as a protein
You can check our Peptides Vs. Proteins page in the peptide glossary to get a more comprehensive explanation of polypeptides, proteins, and peptides.
When a substance comes into contact with water leading to a response), a peptide bond can be broken down by hydrolysis (this is a chemical breakdown process that occurs. While the response isn’t quick, the peptide bonds existing within proteins, polypeptides, and peptides can all break down when they react with water. The bonds are called metastable bonds.
When water responds with a peptide bond, the response launches near to 10kJ/mol of complimentary energy. Each peptide bond has a wavelength absorbance of 190-230 nm.
In the natural universe, enzymes consisted of in living organisms can forming and likewise breaking the peptide bonds down.
Numerous neurotransmitters, hormones, antitumor representatives, and antibiotics are categorized as peptides. Provided the high variety of amino acids they consist of, much of them are considered proteins.
The Peptide Bond Structure
Scientists have actually finished x-ray diffraction studies of various small peptides to help them figure out the physical attributes had by peptide bonds. The research studies have actually shown that peptide bonds are planer and rigid.
The physical appearances are predominantly a repercussion of the amide resonance interaction. Amide nitrogen is in a position to delocalize its particular electrons match into the carbonyl oxygen. The resonance has a direct result on the peptide bond structure.
Undoubtedly, the N-C bond of each peptide bond is, in fact, shorter compared to the N-Ca bond. It also takes place that the C= 0 bond is lengthier compared to the regular carbonyl bonds.
The amide hydrogen and the carbonyl oxygen in a peptide remain in a trans configuration, instead of remaining in a cis setup. A trans configuration is considered to be more dynamically motivating because of the possibility of steric interactions when handling a cis configuration.
Peptide Bonds and Polarity
Typically, free rotation should happen around a given bond between amide nitrogen and a carbonyl carbon, the peptide bond structure. Then again, the nitrogen referred to here only has a singular pair of electrons.
The only pair of electrons lies near to a carbon-oxygen bond. For this reason, it’s possible to draw a sensible resonance structure. It’s a structure where a double bond is utilized to link the carbon and the nitrogen.
As a result, the nitrogen will have a favorable charge while the oxygen will have a negative one. The resonance structure, therefore, gets to prevent rotation about this peptide bond. Additionally, the material structure ends up being a one-sided crossbreed of the two forms.
The resonance structure is deemed a vital factor when it comes to portraying the actual electron circulation: a peptide bond contains around forty percent double bond character. It’s the sole reason that it’s always rigid.
Both charges trigger the peptide bond to get a permanent dipole. Due to the resonance, the nitrogen stays with a +0.28 charge while the oxygen gets a -0.28 charge.
A peptide bond is, hence, a chemical bond that occurs in between two particles. It’s a bond that occurs when a carboxyl cluster of a provided molecule reacts with an amino set from a 2nd particle. The reaction eventually releases a water molecule (H20) in what is called a condensation reaction or a dehydration synthesis reaction.
A peptide bond refers to the covalent bond that gets created by 2 amino acids. From this reaction, a peptide bond gets formed, and which is also called a CO-NH bond. While the response isn’t fast, the peptide bonds existing within polypeptides, proteins, and peptides can all break down when they react with water. The bonds are known as metastable bonds.
A peptide bond is, thus, a chemical bond that occurs between two molecules.
Peptides need correct filtration throughout the synthesis procedure. Given peptides’ complexity, the purification approach used need to portray efficiency.
Peptide Filtration procedures are based on principles of chromatography or crystallization. Formation is frequently utilized on other substances while chromatography is preferred for the purification of peptides.
Elimination of Particular Impurities from the Peptides
The type of research study carried out determines the expected pureness of the peptides. There is a requirement to develop the type of pollutants in the peptides and approaches to eliminate them.
Pollutants in peptides are associated with different levels of peptide synthesis. The filtration methods ought to be directed towards managing particular impurities to satisfy the required standards. The purification procedure involves the isolation of peptides from various compounds and impurities.
Peptide Purification Method
Peptide purification accepts simplicity. The procedure takes place in 2 or more steps where the initial step eliminates the majority of the pollutants. These pollutants are later on produced in the deprotection level. At this level, they have smaller sized molecular weight as compared to their initial weights. The second filtration action increases the level of pureness. Here, the peptides are more polished as the procedure uses a chromatographic principle.
Peptide Filtration Processes
The Peptide Purification process includes units and subsystems which include: preparation systems, information collection systems, solvent delivery systems, and fractionation systems. It is recommended that these processes be carried out in line with the current Good Manufacturing Practices (cGMP).
Affinity Chromatography (A/C).
This purification process separates the peptides from impurities through the interaction of the peptides and ligands. Specific desorption uses competitive ligands while non-specific desorption embraces the change of the PH. Eventually, the pure peptide is collected.
Ion Exchange Chromatography (IEX).
Ion Exchange Chromatography (IEX) is a high capacity and resolution procedure which is based on the differences in charge on the peptides in the mixture to be purified. The prevailing conditions in the column and bind are changed to result in pure peptides.
Hydrophobic Interaction Chromatography (HIC).
A hydrophobic with a chromatic medium surface connects with the peptides. The procedure is reversible and this enables the concentration and filtration of the peptides.
At first, a high ionic strength mix is bound together with the peptides as they are packed to the column. The salt concentration is then lowered to boost elution. The dilution process can be effected by ammonium sulfate on a reducing gradient. Lastly, the pure peptides are collected.
Gel Filtration (GF).
The Gel Filtration purification procedure is based upon the molecular sizes of the peptides and the readily available pollutants. It is efficient in little samples of peptides. The process leads to a good resolution.
Reversed-Phase Chromatography (RPC).
Reversed-Phase Chromatography uses the concept of reverse interaction of peptides with the chromatographic medium’s hydrophobic surface. The RPC technique is relevant during the polishing and mapping of the peptides. The solvents used during the process cause change of the structure of the peptides which impedes the healing process.
Compliance with Great Manufacturing Practices.
Peptide Filtration procedures should be in line with the GMP requirements. The compliance impacts on the quality and purity of the final peptide. According to GMP, the chemical and analytical techniques applied need to be well documented. Appropriate preparation and testing must be accepted to guarantee that the procedures are under control.
The purification phase is amongst the last steps in peptide synthesis. The limitations of the critical criteria should be developed and thought about during the filtration procedure.
The peptide purification procedure is essential and thus, there is a need to adhere to the set guidelines. Therefore, compliance with GMP is crucial to high quality and pure peptides.
Impurities in peptides are associated with different levels of peptide synthesis. The filtration process involves the isolation of peptides from various substances and impurities.
The Peptide Purification process integrates units and subsystems which include: preparation systems, information collection systems, solvent delivery systems, and fractionation systems. The Gel Filtering purification process is based on the molecular sizes of the peptides and the offered impurities. The solvents used during the process cause alteration of the structure of the peptides which impedes the healing process.
Lyophilized is a freeze-dried state in which peptides are usually supplied in powdered form. The process of lyophilization includes eliminating water from a substance by putting it under a vacuum after freezing it– the ice changes from strong to vapour without altering to its liquid state. The lyophilized peptides have a fluffy or a greater granular texture and look that appears like a small whitish “puck.” Numerous techniques used in lyophilization methods can produce more compacted or granular in addition to fluffy (voluminous) lyophilized peptide.
Before using lyophilized peptides in a lab, the peptide needs to be reconstituted or recreated; that is, the lyophilized peptide ought to be liquified in a liquid solvent. However, there does not exist a solvent that can solubilize all peptides as well as preserving the peptides’ compatibility with biological assays and its stability. In many circumstances, distilled, sterile along with normal bacteriostatic water is used as the first choice while doing so. Regrettably, these solvents do not liquify all the peptides. Looks into are generally required to use a trial and error based approach when trying to reconstruct the peptide utilizing a significantly more potent solvent.
In this regard, acidic peptides can be recreated in important services, while standard peptides can be rebuilded in acidic options. Neutral peptides and hydrophobic peptides, which consist of vast hydrophobic and uncharged polar amino acids, respectively, need natural solvents to recreate.
Peptides with complimentary cysteine or methionine ought to not be reconstructed using DMSO. This is due to side-chain oxidation happening, which makes the peptide unusable for lab experimentation.
Peptide Recreation Standards
As a first guideline, it is suggested to utilize solvents that are easy to remove when dissolving peptides through lyophilization. This is taken as a preventive procedure in the event where the very first solvent utilized is not adequate. The solvent can be eliminated utilizing the lyophilization procedure. Scientists are advised first to try dissolving the peptide in normal bacteriostatic water or sterile distilled water or dilute sterilized acetic acid (0.1%) solution. It is likewise advisable as a basic guideline to check a percentage of peptide to identify solubility prior to trying to dissolve the entire part.
One important reality to think about is the preliminary use of dilute acetic acid or sterile water will enable the scientist to lyophilize the peptide in case of failed dissolution without producing unwanted residue. In such cases, the scientist can attempt to lyophilize the peptide with a stronger solvent once the inadequate solvent is eliminated.
The scientist ought to attempt to liquify peptides using a sterilized solvent producing a stock service that has a greater concentration than required for the assay. When the assay buffer is utilized initially and fails to dissolve all of the peptides, it will be hard to recuperate the peptide without being untainted. The procedure can be reversed by diluting it with the assay buffer after.
Sonication is a process utilized in laboratories to increase the speed of peptide dissolution in the solvent when the peptides persist as a whitish precipitate noticeable inside the service. Sonication does not change the solubility of the peptide in a solvent however merely assists breaking down pieces of strong peptides by quickly stirring the mix. After finishing the sonication process, a scientist needs to examine the service to find out if it has gelled, is cloudy, or has any type of surface area scum. In such a scenario, the peptide might not have dissolved but stayed suspended in the solution. A stronger solvent will, therefore, be necessary.
Practical laboratory implementation
In spite of some peptides needing a more potent solvent to fully liquify, typical bacteriostatic water or a sterilized pure water solvent works and is the most frequently used solvent for recreating a peptide. As pointed out, sodium chloride water is extremely prevented, as pointed out, considering that it tends to trigger precipitation with acetate salts. A basic and easy illustration of a common peptide reconstitution in a laboratory setting is as follows and is not distinct to any single peptide.
* It is crucial to enable a peptide to heat to room temperature level prior to taking it out of its product packaging.
You may likewise opt to pass your peptide mixture through a 0.2 micrometre filter for germs avoidance and contamination.
Using sterilized water as a solvent
- Step 1– Take off the peptide container plastic cap, hence exposing its rubber stopper.
- Action 2– Take off the sterile water vial plastic cap, hence exposing the rubber stopper.
- Action 3– Utilizing alcohol, swab the rubber stoppers to prevent bacterial contamination.
- Step 4– Draw 2ml of water from the sterilized water container.
- Step 5– Gradually pour the 2ml of sterilized water into the peptide’s container.
- Action 6– Swirl the solution carefully until the peptide dissolves. Please avoid shaking the vial
Prior to utilizing lyophilized peptides in a laboratory, the peptide has actually to be reconstituted or recreated; that is, the lyophilized peptide must be dissolved in a liquid solvent. Neutral peptides and hydrophobic peptides, which consist of huge hydrophobic and uncharged polar amino acids, respectively, require natural solvents to recreate. Sonication is a process utilized in laboratories to increase the speed of peptide dissolution in the solvent when the peptides persist as a whitish precipitate noticeable inside the option. Sonication does not modify the solubility of the peptide in a solvent but merely helps breaking down pieces of strong peptides by quickly stirring the mixture. Despite some peptides needing a more powerful solvent to totally liquify, common bacteriostatic water or a sterile distilled water solvent is effective and is the most typically used solvent for recreating a peptide.
Pharmaceutical grade Peptides can be used for various applications in the biotechnology industry. The availability of such peptides has made it possible for researchers and biotechnologist to conduct molecular biology and pharmaceutical development on an expedited basis. A number of business offer Pharmaceutical grade Peptides peptide synthesis services to satisfy the needs of the customers.
It is derived from a particle that consists of a peptide linkage or a residue that binds to a peptide. Biological function of peptide can be realised through Pharmaceutical grade Peptides peptide synthesis. Biochemical process is understood through the usage of peptide synthesis.
Pharmaceutical Peptide Synthesis
The primary purpose of peptide synthesis is the manufacture of anti-microbial agents, antibiotics, insecticides, hormonal agents, enzymes and vitamins. The process of synthesis of peptide includes several steps consisting of peptide isolation, gelation, purification and conversion to a beneficial type.
There are lots of kinds of peptide offered in the market. They are identified as follows: peptide derivatives, non-peptide, hydrolyzed, hydrophilic, and polar. These classifications consist of the most typically utilized peptide and the process of producing them.
Non-peptide peptide derivatives
Non-peptide peptide derivatives consist of C-terminal pieces (CTFs) of the proteins that have been treated chemically to remove side impacts. Some of these peptide derivatives are derived from the C-terminal pieces of human genes that are utilized as genetic markers and transcription activators.
Porphyrins are produced when hydrolyzed and then transformed to peptide through peptidase. Porphyrin-like peptide is obtained through a series of chemical processes.
Disclaimer: All items listed on this website and offered through Pharma Labs Global are meant for medical research purposes just. Pharma Lab Global does not promote the usage or motivate of any of these products in a personal capacity (i.e. human usage), nor are the products meant to be utilized as a drug, stimulant or for usage in any food.
Several companies supply Pharmaceutical grade Peptides peptide synthesis services to fulfil the requirements of the clients.
It is derived from a molecule that consists of a peptide linkage or a residue that binds to a peptide. Biological function of peptide can be realised through Pharmaceutical grade Peptides peptide synthesis. Biochemical process is realised through the usage of peptide synthesis.
The procedure of synthesis of peptide involves several actions consisting of peptide isolation, filtration, conversion and gelation to an useful type.
Peptides in WikiPedia
Peptides (from Greek language πεπτός, peptós “digested”; stemmed from πέσσειν, péssein “to digest”) are short chains of between 2 and fifty amino acids, linked by peptide bonds. Chains of less than ten or fifteen amino acids are called oligopeptides, and also include dipeptides, tetrapeptides, and tripeptides.
A polypeptide is a longer, continual, unbranched peptide chain of approximately about fifty amino acids. Thus, peptides fall under the wide chemical courses of biological polymers and also oligomers, together with nucleic acids, polysaccharides, others, and oligosaccharides.
A polypeptide that consists of greater than approximately fifty amino acids is known as a protein. Healthy proteins contain several polypeptides organized in a biologically functional way, commonly bound to ligands such as coenzymes and cofactors, or to one more healthy protein or other macromolecule such as DNA or RNA, or to intricate macromolecular assemblies.Amino acids that have been integrated right into peptides are labelled deposits. A water molecule is launched during formation of each amide bond. All peptides except cyclic peptides have an N-terminal(amine group) as well as C-terminal(carboxyl group)deposit at the end of the peptide (as revealed for the tetrapeptide in the image).
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domain: biology