Ford Fiesta axed from line-up
Production of the Blue Oval’s iconic supermini will end in June 2023 as Ford confirms Fiesta’s demise
It has been confirmed that the Ford Fiesta supermini will be axed from Ford’s line-up, with production of the brand’s compact hatchback scheduled to end in June 2023.
In an official statement Ford of Europe President Martin Sander said: “At Ford in Europe, we are accelerating our efforts to go all-in on electrification with our passenger vehicles being fully electric by 2030 – and all vehicles across our Ford portfolio by 2035. As we get ready to transition to an electric future, we will discontinue production of S-Max and Galaxy in Valencia, Spain in April 2023 and discontinue Fiesta production in Cologne, Germany by end of June 2023.
“We will introduce three new exciting electric passenger vehicles and four new electric commercial vehicles in Europe by 2024. We plan to sell more than 600,000 electric vehicles in the region by 2026, and the electric passenger vehicle production at the Cologne Electrification Centre will reach 1.2 million vehicles over a six-year timeframe.”
Eerder gaf Ford al aan dat de Ford Focus productie in 2025 zal eindigen. Klik
Ford is undergoing a huge transition towards a fully electric line-up and confirmed in March this year that the Fiesta’s bigger brother, the Puma compact SUV, will be available as a fully-electric car by 2024. This is one of three new electric passenger vehicle’s mention in Sander’s statement, along with two new SUVs – a more practical model and a sportier crossover – both based on the Volkswagen Group MEB platform as part of a tie-up between the two companies.
It’s understood that the Puma EV will share components – including elements of its platform – with the Transit Courier and Tourneo Courier electric vans (both part of Ford’s electric commercial vehicle push in Europe) in order to keep pricing affordable. The electric Puma will be
built at the same factory as the commercial vehicles in Craiova, Romania.
However, although the combustion-engined Puma shares its Global B Small Car platform with the Fiesta, there are no plans for a Fiesta EV on this new, more affordable platform.
Although this would in theory help keep the cost of electrifying Ford’s small cars down, a problem many brands are tackling at the moment with models such as the future reborn Renault 5 EV and the Volkswagen Group’s ‘MEB Entry’ cars, the VW ID. Life, Cupra UrbanRebel and Skoda’s forthcoming model on this platform, profitability in this segment is not as great as with fully electric SUVs.
It’s thought that part of the reason for axing the Fiesta in the first place is due to the rising cost of small cars and sliding sales in this sector compared with more popular small SUVs and crossover models.
Ford’s switch to electric mobility will see its Cologne plant – where the Fiesta is currently built – turned into an EV manufacturing hub that will produce the Ford-badged version of the MEB-based cars mentioned above. The first Ford model using this tech is set to go into production next year.
The Fiesta is Britain’s best-selling car ever having shifted nearly five million units over its 46-year lifespan so far. The car routinely topped the Society of Motor Manufacturers and Traders annual registration charts until it was recently overtaken by the Vauxhall Corsa.
The Fiesta didn’t feature in the 2021 top 10 chart and, year-to-date, despite a facelift in late 2021 the model doesn’t feature in the 2022 top 10 list either. However, Ford’s Puma does rank in this year’s list up to the end of September, in third spot with 27,680 registrations.
Earlier this year Ford suspended orders for its updated Fiesta (along with the newly facelifted Focus and the Galaxy and S-Max MPVs) citing supply chain issues.
Although both the Fiesta and Focus are back on sale, with greater profit margins on its more popular SUV models such as the Puma and Kuga (the latter seventh in the registration chart year-to-date) it’s likely that Ford has prioritised production of these cars to boost profitability, underpinning the decision to axe the Fiesta and continue on with Puma sales as the entry point to the firm’s line-up, along with the EcoSport compact SUV.
Source | AutoExpress.co.uk


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A kingfish when you look at the Kansas City high-interest loan industry will minimize attempting to gather on huge number of unlawful, high-interest loans built to bad New Yorkers, under money announced Monday because of the state dept. of Financial Solutions.
Total Account Healing and E-Finance Call Center Help
But, you will have no refunds for folks who already made payments for a long time to either for the two Kansas-based businesses вЂ” Total Account healing and E-Finance Call Center help.
Both businesses are element of the alleged loan that is”payday industry, which lends money quickly at excessive short-term interest levels which are unlawful under usury legislation in nyc along with other states. Ny caps yearly rates of interest at 25 %.
Pay day loans are applied for by bad residents whom may well not be eligible for a conventional loans from banks. The loans are a definite $38 billion industry nationwide, and high interest rates make such loans extremely lucrative for loan providers, based on the Pew Charitable Trust.
Relating to state Superintendent Maria T. Vullo, complete Account Recovery obtained loan that is illegal from significantly more than 2,100 New Yorkers between 2011 and 2014. The division failed to indicate exactly just how money that is much collected.
“Payday financing is unlawful in ny, and DFS will not tolerate predatory actors within our communities,” stated Vullo’s statement. Altogether, the ongoing organizations desired re re payments on 20,000 loans from throughout the state.
Both businesses are linked with Joshua Mitchem, a Kansas City guy that is a major player in the industry, together with his daddy, Steve Mitchem, an old traveling evangelist and luxury precious precious precious jewelry professional whom 10 years ago founded pay day loan companies into the Kansas City region. The elder Mitchem happens to be wanting to take advantage of the medical cannabis sector.
In 2012, Joshua Mitchem ended up being sued by the Arkansas Attorney General for violating state laws that are usury recharging rates of interest greater than 500 % on loans. That lawsuit advertised Mitchem went the companies through a number of shell corporations into the Caribbean. Mitchem later on paid an $80,000 fine and decided to stop company for the reason that state.
Underneath the settlement in nyc, Mitchem’s organizations can pay a $45,000 state penalty, and consented to stop customers that are pursuing about $12 million in unlawful loans, along with to withdraw.
Judgments and liens filed against debtors
Nevertheless, unlike the very last major nyc state settlement with another pay day loan operator in might 2016, you will see no refunds for clients whom already made re re online payday loans Utah payments to Mitchem’s organizations through July 2014, when his two organizations presumably ceased wanting to gather in nyc.
- Most readily useful purchase at Crossgates abruptly closed
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- Bethlehem manager calls BLM that is young activist expletive at town conference
- Despite ‘strict’ freeze, Cuomo keeps employing for their workplace
If the division had been expected why refunds are not the main settlement, Vullo issued a declaration having said that the department “considers all appropriate facets when selecting a suitable strategy.”
Based on the settlement finalized by Joshua Mitchem, the businesses have actually a “diminished monetary condition” that produces the firms unable “which will make re payment of monies” beyond their state fine.
But, since early 2015 Mitchem has donated significantly more than $20,000 in governmental campaign efforts, including into the election campaign of President Donald J. Trump; an action that is political connected to Trump’s option to go the U.S. Environmental Protection Agency, previous Oklahoma Attorney General Scott Pruitt; and a trade team for payday financing.
This past year, federal regulators regarding the Obama-era Consumer Protection Board proposed nationwide guidelines when it comes to industry, that has been mostly managed by specific states. Kansas City is actually a center for pay day loan businesses just like the Mitchems’.
President Trump’s proposed federal spending plan would slash capital in the customer Protection Bureau, that could undercut federal efforts to regulate payday financing, that your industry vehemently opposes.

domain: finance


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Contains leaflets, flyers, posters, publications and ephemera. Gathered by the Social Protest Project primarily on Sproul
Plaza for the UC undergraduate library between 1969-1982, the material primarly relates to the Vietnam War and Civil Rights
demonstrations although there is significant material on Black Power, political issues, the Women's movement, lesbian and
gay rights, Third World issues, the Left and Right, campus labor disputes, and the movement against nuclear power and nuclear
The following chronology, compiled by Professor William Rorabaugh of the University of Washington, provides an overview of
key protest dates in the Bay Area.
Number of containers: 27 cartons, 2 oversize boxes, 1 card file box
Linear feet: 33.87
Microfilm copies: 101 reels : positive and negative (BNEG 3106) ; 35 mm
10 digital objects (11 images)
Materials in this collection may be protected by the U.S. Copyright Law (Title 17, U.S.C.). In addition, the reproduction
of some materials may be restricted by terms of University of California gift or purchase agreements, donor restrictions,
privacy and publicity rights, licensing and trademarks. Transmission or reproduction of materials protected by copyright beyond
that allowed by fair use requires the written permission of the copyright owners. Works not in the public domain cannot be
commercially exploited without permission of the copyright owner. Responsibility for any use rests exclusively with the user.
RESTRICTED ORIGINALS. Use microfilm copy with call number BANC FILM 2757. Collection is open for research except for the unprocessed
serials. Inquiries concerning these should be directed to the Head of Public Services of The Bancroft Library.

domain: library


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text:
WADA’s move shows trust in Russia’s anti-doping measures — ministerSport June 28, 1:02
US disciplinary procedure against jailed Russian businessman Bout delayed — attorneyWorld June 27, 23:16
FIFA report on Russia’s 2018 World Cup bidding proves legitimacy of its win — deputy PMSport June 27, 21:08
FIFA report on Russia’s 2018 bidding dismisses Western media allegations — LOC chiefSport June 27, 19:53
Encrypting ransomware Petya attacks computers worldwide — Kaspersky LabBusiness & Economy June 27, 19:23
Kremlin says its computers not affected by hacker attackRussian Politics & Diplomacy June 27, 18:55
Security experts urge Putin, Trump to overcome disagreementsWorld June 27, 18:51
Jury to deliver verdict on Nemtsov murder case on June 28Society & Culture June 27, 18:42
Syrian president visits Russia’s Khmeymim airbaseWorld June 27, 18:17
LUHANSK, October 30. /TASS/. The armed conflict in the self-proclaimed Luhansk People’s Republic (LPR) has killed 456 civilians and wounded another 2,500 since May, the local health minister said on Thursday.
“Since May, the total number of those affected has reached 2,973, with 2,514 of them wounded. Another 456 people have been killed as a result of armed actions,” Larisa Ayrapetyan, who is running for the head of the self-proclaimed republic on November 2, said.
The United Nations said as of October 21, a total of 3,724 people have been killed and 9,205 others wounded during the armed conflict in eastern Ukraine. The United Nations Office for the Coordination of Humanitarian Affairs (OCHA) said 5.2 million people currently remain in the conflict zone.
Some 830,000 citizens have fled Ukraine for Russia, while 450,000 of them have applied for a refugee status or temporary asylum, Russia’s Deputy Permanent Representative to the UN Yevgeny Zagaynov said.

domain: news


A federal choose has granted the New York legal professional basic’s request to maneuver a lawsuit in opposition to Amazon again to New York state court docket, Reuters reported. AG Letitia James’ lawsuit alleges that the ecommerce large failed to guard staff at its Brooklyn and Staten Island warehouses from COVID-19 in the course of the early months of the pandemic, and retaliated in opposition to staff who complained concerning the lack of security measures.
Amazon had sought to have the case moved to federal court docket, arguing that office issues of safety weren’t underneath James’ purview. The corporate pre-emptively sued James on February 12th, and James sued Amazon 4 days later. Amazon then moved the lawsuit to federal court docket.
“Amazon has compelled its staff to work in unsafe circumstances all through this pandemic,” James mentioned in a press release following US District Decide Jed Rakoff’s order. “We’re happy with right now’s determination to permit this case to be heard in state court docket, the place it belongs.”
The ruling didn’t tackle the deserves of the lawsuit, which seeks improved circumstances for staff and damages for 2 Amazon staff who say the corporate retaliated in opposition to them.
Staff at Amazon’s JKF8 warehouse in Staten Island mentioned final March that they didn’t have vital protecting gear. Amazon fired a number of staff who protested the circumstances, together with Chris Smalls, who had organized a walkout.
On the time, James known as Smalls’ firing “disgraceful” and pushed for an investigation by the Nationwide Labor Relations Board. Amazon mentioned on the time that the employees “weren’t terminated for speaking publicly about working circumstances” however for violating security insurance policies.
Amazon didn’t instantly reply to a request for remark from The Verge on Saturday.


Tour Dates 2021
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NEW YORK (TheStreet) -- Wall Street and Republicanism go together like peanut butter and jelly. It's been that way for over 150 years.
But the re-authorization of the Export-Import Bank, which has helped companies finance export deals since the Franklin D. Roosevelt administration, could push some to consider divorce.
The bank says it supports 1.2 million jobs through its financing, most of which it says is made on behalf of small businesses that might otherwise be unable to do the deals.
But some very big companies also use the bank such as Boeing (BA), which Standard & Poor's recently warned could have its long-term credit rating downgraded if the bank is closed. Shares in General Electric (GE) and Caterpillar (CAT) could also be impacted if the charter is not renewed.
A recent article in an American Enterprise Institute publication by James DeLong offers a good summary. The headline is Why Voters Grew Tired of Cantor. The URL reads government-of-the-cronies-by-the-cronies-for-the-cronies.
Senate Democrats are trying to tie a short-term re-authorization of the bank to their version of a government funding bill, which would force House Republicans to close the government on Sept. 30, a month before the election, if they want to close the bank.
There are some Democrats who oppose re-authorization, but most seem to see it as an opportunity to renew ties with Wall Street and other business interests. They think a government shutdown could make October very interesting, and I agree.
A who's who of Wall Street editorialists -- from The Wall Street Journal and Financial Times to The New York Times and Bloomberg -- have recently pounded the table for the bank. The U.S. Chamber of Commerce has also issued a call to re-authorize the bank.
The problem is these groups have no influence over the Tea Party Republicans who oppose the bank, and with new House Majority Leader Kevin McCarthy of California opposing re-authorization, some may face a hard choice.
The question becomes how these business groups will react if Republicans kill the goose they say lays golden eggs. Will they take it or will they open their wallets to Democrats and fight back? If they do decide to fight it may prove the most important political and economic story of 2014.
At the time of publication the author owned shares of BA and GE.
This article represents the opinion of a contributor and not necessarily that of TheStreet or its editorial staff.

domain: politics


text:
Telecom Lead Asia: Nokia Siemens Networks — in its annual report — said its India revenue declined 19.1 percent to €737 million in 2012 from €911 million in 2011 and €885 million in 2010.
Nokia Siemens Networks’ number of employees in December 2012 stood at 10,192 in India against 58,411 globally. In 2010, the global employee base was 66,160.
Revenue of Ericsson, main rival in India, declined 34 percent to SEK 6.5 billion, primarily due to less Capex spend by local mobile service providers.
Nokia Siemens Networks — in its annual report — said 2 percent dip in global net sales was primarily due to modifying its structure to align with its strategy to focus on mobile broadband as it streamlined its portfolios, divested non-core businesses and exited from loss-generating and poorly performing contracts and countries.
Of total net sales, mobile broadband contributed €6 billion in the year ended December 31, 2012 (€6.3 billion in 2011) and global services contributed €6.9 billion in 2012 (€6.7 billion in 2011).
Japan accounted for largest concentration of net sales in 2012, representing 16.3 percent of net sales (11.2 percent in 2011).
Other regions contributing signiﬁcant percentages of net sales in the year ended December 31, 2012 include West Europe, representing 14.2 percent of net sales (14.9 percent in 2011), and Latin America representing 12.3 percent of net sales (12.9 percent in 2011).
On a regional basis, net sales in the year ended December 31, 2012 were driven primarily by strength in our Asia and Middle East region, most notably Japan, which saw an increase in net sales of 41.7 percent due to strong growth in sales of both infrastructure equipment and services as a result of the 4G / LTE rollouts by mobile operators in Japan, which represented a combination of organic growth and the impact of the Acquired Motorola Assets.
According to Nokia Siemens, these positive developments were partially offset by decreases in net sales in India and Greater China as a result of reduced operator spending.
Its net sales in the Americas region also increased 2.6 percent, led by an increase in net sales in North America of 18.0 percent driven by the 4G / LTE network rollout with customer T-Mobile USA partially offset by a decrease in net sales in Latin America.
Overall growth in net sales in these regions was offset by lower sales in our Europe and Africa region, which overall decreased by 12.0 percent in 2012 as compared to 2011, due to the 19.5 percent and 17.3 percent decline in net sales in North East Europe and South East Europe, respectively, principally as a result of lower sales in services and infrastructure equipment.

domain: geography


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Cells and cell lines
Fibroblasts from individuals heterozygous for BRCA2 999del5 were grown in RPMI 1640 (Invitrogen Life Technologies, Carlsbad, CA, USA) supplemented with 20% FCS, 100 U/ml penicillin and 100 μg/ml streptomycin. Overexpressions were performed in COS7 cells (ATCC, Rockville, MD, USA) grown in DMEM (Invitrogen Life Technologies) supplemented with 10% FCS, 100 U/ml penicillin and 100 μg/ml streptomycin.
RNA isolation, cDNA synthesis and cloning of BRCA2 999del5
Total RNA was isolated using the TRIZOL Reagent (Invitrogen Life Technologies). Three micrograms of total RNA were used for cDNA synthesis (First-Strand cDNA Synthesis kit; Amersham Biosciences, Hilleröd, Denmark). The open reading frame of BRCA2 999del5 was amplified using a primer pair where the 5' primer was complementary to the Kozak sequence of the BRCA2 cDNA and the 3' primer covered a 22 bp region prior to the putative stop codon of the BRCA2 999del5 cDNA. The sequences were GTAAAAATGCCTATTGGATCC for the 5' cloning primer, and AATGAATTCCCTGATGTTTTTC for the 3' cloning primer.
The reaction mixture contained 2 μl cDNA template, 2 mM MgCl2, 15 nmol dNTPs, 15 pmol each primer and 2 U Taq polymerase. The reaction proceeded at 95°C for 5 min, followed by 40 cycles of 94°C for 1 min, 55°C for 45 s and 72°C for 1 min, and finally heating to 72°C for 5 min and cooling to 4°C.
The amplified BRCA2 product was isolated by electrophoresis on a 1% agarose gel, and then the DNA purified (GFX PCR DNA and Gel Band Purification Kit; Amersham Biosciences), cloned into pCR2.1-TOPO vector (Invitrogen Life Technologies) and used to transform TOP10 bacteria. Plasmids were purified from individual bacterial clones (Qiagen Plasmid purification Kit; Qiagen GmbH, Hilden, Germany) and the clones were sequenced (ABI PRIZM Big Dye Terminator Cycle Sequencing Ready Reaction kit; Applied Biosystems, Foster City, CA, USA). After the deletion had been confirmed, the mutated DNA insert was excised from the TOPO-TA plasmid with HindIII and NotI restriction enzymes (New England Biolabs, Beverly, MA, USA) and ligated into the pIND(SP1)/V5-His expression vector (Invitrogen Life Technologies).
Transfections and activation of expression
Cells were grown to 60–65% confluency. Four microliters of FuGENE 6 reagent (Roche Applied Science, Hvidovre, Denmark) were added to 250 μl serum-free medium and the mixture was incubated for 5 min at room temperature. After the addition of plasmid DNA (2 μg per transfection), the mixture was incubated for 20 min at room temperature and then added to the cell cultures that had received fresh serum-containing medium. The cell cultures were allowed to recover after transfection for 24 hours, fresh medium was added and the cells were subsequently activated with ponasterone A (Invitrogen Life Technologies) at a concentration of 2.5 μg/ml, as recommended by the manufacturer.
Cell lysis, precipitations, and Western blotting
Cells were lysed in RIPA lysis buffer containing 1% Triton X-100, 1% deoxycholic acid, 20 mM Tris–HCl (pH 7.4), 150 mM NaCl, 2.5 mM EDTA, 10% glycerol and 10 mM Na3P2O7, as well as 160 inhibitory units/ml aprotinin and 0.5 mM phenylmethylsulfonyl fluoride. After lysis, 10% of the lysate was removed (for use as a control) and the rest was incubated with antibody. For immunoprecipitations, 2.5 μl primary monoclonal antibody or 5 μl primary polyclonal antibody were used per precipitation. After the addition of antibody, the samples were subjected to gentle end-over-end shaking for 1–2 hours at 4°C. Subsequently, 50 μl Protein G Sepharose beads (Amersham Biosciences) were added to each sample and the mixture incubated for a further 1 hour at 4°C. The beads were washed with lysis buffer, the supernate was removed and 50 μl of 2 × sample buffer containing β-mercaptoethanol were added to the beads. The samples were boiled for 5 min and were centrifuged for 30 s.
For precipitation of the overexpressed 6 × His-tagged proteins, Ni-NTA Magnetic Agarose Beads (Qiagen GmbH) were used. For purification of the 6 × His-tagged proteins under native conditions, cells transiently transfected with pIND-BRCA2 999del5 or with pIND-LacZ were lysed in lysis buffer containing 50 mM NaH2PO4 (pH 8), 300 mM NaCl, 10 mM imidazole, 1% Tween 20. Ten microliters of 5% Ni-NTA Magnetic Agarose Bead suspension were added to the lysate and incubated for 1–2 hours at 4°C. The supernate was separated from the beads using a Qiagen 12-Tube Magnet (Qiagen) and the beads were washed several times with washing buffer containing 50 mM NaH2PO4 (pH 8), 300 mM NaCl, 20 mM imidazole, 0.05% Tween 20. The proteins were eluted from the beads with 25 μl elution buffer containing 50 mM NaH2PO4 (pH 8), 300 mM NaCl, 250 mM imidazole, 0.05% Tween 20, an equal volume of 2 × sample buffer supplemented with β-mercaptoethanol was added to the eluates, and the samples were boiled for 5 min and centrifuged for 30 s.
Purification of the 6 × His-tagged proteins under denaturing conditions proceeded in exactly the same way except that the cells were lysed in a buffer containing 8 M urea, 0.1 M NaH2PO4, 0.01 M Tris–Cl (pH 8), that the agarose beads with the bound 6 × His-tagged proteins were washed with a buffer containing 8 M urea, 0.1 M NaH2PO4, 0.01 M Tris–Cl (pH 6.3), and that a buffer containing 8 M urea, 0.1 M NaH2PO4, 0.01 M Tris–Cl (pH 4.5) was used for elution of the proteins from the beads.
The precipitates and the lysates were separated by electrophoresis on gradient 6–18% polyacrylamide gels and blotted onto a Hybond-P membrane (Amersham Biosciences). The primary antibodies used in this study were: SC1 and SC3 rat monoclonal antibodies raised against the N-terminal and C-terminal sequences of BRCA2, respectively (a generous gift from D Bertwistle and A Ashworth); anti-V5 mouse monoclonal antibody (Invitrogen Life Technologies); anti-actin mouse monoclonal antibody (SDS); anti-BRCA2 rabbit polyclonal antibody raised against the putative out-of-frame residues at the C-terminus of BRCA2 999del5 (Bethyl Laboratories, Montgomery, TX, USA); and NCL-p53-DO7 mouse monoclonal antibody against p53 (Novocastra, Newcastle, UK). Secondary peroxidase-conjugated donkey anti-mouse and donkey anti-rabbit horseradish antibodies were purchased from Amersham Biosciences and peroxidase-conjugated goat anti-rat antibody was purchased from Pierce Biotechnology (Rockford, IL, USA). The ECL detection system (Amersham Biosciences) was used to produce images.
Total RNA samples (about 15 μg each) were separated by electrophoresis on a 1% agarose gel containing 2.2 M formaldehyde and 0.02 M 3-(N-morpholino)propanesulfonic acid, stained with ethidium bromide and visualized by UV. The gels were blotted onto Hybond-N+ membrane (Amersham Biosciences) and RNA was subsequently fixed by exposing the membrane to UV light for 5 min. The BRCA2 999del5 insert of the pIND-BRCA2 999del5 plasmid was excised from the vector with HindIII and NotI. Then 100 ng insert were labeled using the ECL direct nucleic acid labeling system (Amersham Biosciences) and were used as a probe. The labeled probe was added to a hybridization buffer containing 0.5 M NaCl and 5% blocking agent, and the mixture was incubated with the membrane overnight at 42°C. The images were developed using the ECL detection system (Amersham Biosciences).
F96 Maxisorp immunoplates from Nunc (Roskilde, Denmark) were coated with cell lysates. The synthetic 16 amino acid peptide alone (the predicted 16 out-of-frame amino acid stretch of BRCA2 999del5), and the same peptide conjugated to keyhole limpet hemocyanin carrier protein were used as controls. Briefly, wells were coated with different dilutions (1:10, 1:100, 1:1000) of lysates prepared from the heterozygous fibroblasts on one hand, and from homozygous wt fibroblasts on the other hand, all made in duplicate. About 250 ng synthetic peptides were used to coat each control well. All the samples were diluted in coating buffer containing 0.015 M Na2CO3 0.035 M NaHCO3, and 0.003 M NaN3 (pH 9.6), which was also used as a blank. The same experiment was performed with the lysates of the COS7 cells transfected with pIND-BRCA2 999del5 or with pIND-LacZ as control. The antibody against the putative 16 out-of-frame amino acid stretch of BRCA2 999del5 (dilution 1:2500) was used to detect the presence of the mutated BRCA2 protein. Preimmune serum was used as a control. The secondary antibody used was goat anti-rabbit alkaline phosphatase-conjugated antibody (DAKOCytomation, Glostrup, Denmark) diluted 1:3000 in PBS–0.05% Tween 20.
To investigate whether the proteasome plays a role in the instability of BRCA2 999del5 protein, the proteasome inhibitor lactacystin was used to treat cells overexpressing pIND-BRCA2 999del5 or pIND-LacZ. Briefly, 24 hours after transfection, the cells were treated with ponasterone A and consequently with lactacystin at a concentration of 10 μM for different periods of time. The cells were thereafter lysed and subjected to electrophoresis on gradient 6–18% polyacrylamide gel, transferred onto Hybond-P membrane and blotted with α-V5 or with the antibody against the nonrelevant residues of BRCA2 999del5 or SC1 antibody. Blotting with α-actin was performed as a control for protein loading, and blotting with α-p53 was performed as a control for proteasome inhibition.

domain: medical


text:
The use of ethnographic research - social research based on the observation of individuals or institutions where the researcher becomes part of the group or very close to the group to better understand their actions - is becoming more and more of a prevalent methodology within sociology. As ethnography gains prominence within the discipline its focus, theoretical underpinnings and narrative styles are also expanding to the yet-unexamined worlds and institutions of society. Politics, political institutions, and those working in politics (state officials, politicians and activists) have so far missed the lens of the ethnographer. As a group, politicians and those in politics can be found in every corner of the world. While political systems and politicians are by no means the same in every country, what brings these people together to be part of the political process? Ethnography is uniquely equipped to look microscopically at the foundations of political institutions and their attendant sent of practices, just as it is ideally suited to explain why political actors behave the way they do and to identify the causes, processes and outcomes that are part and parcel of political life. The volume, based on a special issue of Qualitative Sociology has a two-fold purpose: to bring politics into the ethnographic literature and of ethnography in studies of politics. The case studies included are based on the research of ethnographers studying the various level of politics in Brazil, Japan, El Salvador, Bosnia, the Philippines, India and the United States. It will be of interest to those in the sociology of politics, political science and those looking for ethnographic research on a global level. TOC:The Difference Ethnography Can Make.- Shame and Worker Activism: Emotional Dynamics in Face-to-Face Encounters.- A Calmly Disruptive Insider: The Case of an Institutionalized Advocacy Organization at Work.- Radical Outcasts versus Three Kinds of Police: Constructing Limits in Japanese Anti-Emperor Protests.- Changing Meanings of Authority in Contemporary Rural India.- Why Social Capital Subverts Institution Building in Risky Settings.- How Local Social Movement Groups Handle a Presidential Election.- Trouble en Route: Drug Trafficker and Clientelism in Rio de Janeiro Shantytowns.- Politics as a Vocation: Notes Toward a Sensualist Understanding of Political Engagement.- The Ethical Challenges of Field Research in Conflict Zones.- Afterword: Political Ethnography as Art and Science.
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domain: sociology


- Can you run 92 octane on a 93 octane tune?
- Is the Diablo tune the 93 octane tune?
- Who has the highest quality gasoline?
- Why is there no 93 octane in California?
- Is 91 octane better for your car?
- Will 93 octane hurt my engine?
- Is 93 a premium gas?
- Does 93 octane have ethanol?
- Can I use 91 octane instead 93?
- Does 93 octane give more power?
- Does 98 octane clean the engine?
- Can octane booster damage an engine?
- Is Shell gas Really Better?
- Does premium gas give better mileage?
- How much HP does 93 octane tune add?
- Does premium gas last longer?
- Who has the best 93 octane gas?
- Can you run 87 octane on a 93 octane tune?
- What gas is better 91 or 93?
- Does 93 octane give better mpg?
- Can high octane fuel damage an engine?
Can you run 92 octane on a 93 octane tune?
You should be fine running 92 octane with the 93 tune.
The octane level of the fuel available is based on things like the altitude and humidity in the region.
The higher the altitude or higher humidity, the lower the combustion temperatures are, therefore less chance for pre-ignition and no need for higher octane fuel..
Is the Diablo tune the 93 octane tune?
RE: Diablo tune is 93 octane tune? Diablo Tune is 93 tune.
Who has the highest quality gasoline?
ChevronOf America’s largest nationwide chains, Chevron scores the highest points in overall customer satisfaction. Its reach spans over 7,800 stores, and while some gas stations offer convenient food marts, one location in North Hollywood goes above and beyond gasoline.
Why is there no 93 octane in California?
The real reason is a bit simpler. Too many executives in CA driving cars that need premium gas. That’s why most cars that say it needs premium have 91 as a minimum octane. On the east coast you can mix some 93 with 87 to get 91.
Is 91 octane better for your car?
But take note: the recommended gasoline for most cars is regular octane. In fact, in most cases, using a higher octane gasoline than your owner’s manual recommends offers absolutely no benefit. It won’t make your car perform better, go faster, get better mileage, or run cleaner.
Will 93 octane hurt my engine?
If you usually fill your tank up with 87-octane gasoline and you accidentally put in a higher octane blend (say, 91, 92, or 93), don’t worry. You’re actually filling your car or truck with a different blend of gas, which means it will burn differently in your engine.
Is 93 a premium gas?
Premium gasoline is generally considered any type of gasoline with an octane level of 91 or greater, with 91 octane and 93 octane being the most common versions of premium gasoline available at gas stations in the United States (93 octane gasoline may be called “ultra” or “super-premium” in some cases).
Does 93 octane have ethanol?
No. All gasoline brands have both pure and ethanol-containing gasoline under the same brand names. For example, Shell V-Power ranges from 91 to 93 octane both with and without added ethanol. It just varies from station to station, and it’s up to the station owner whether or not to sell pure gas.
Can I use 91 octane instead 93?
Higher Octane Doesn’t Mean Better Performance Using a higher octane gasoline will not help clean your engine. … This means that your engine will be cleaned the same by 87 octane fuel as it will by 93 octane fuel, so don’t feel that it’s necessary to treat your car to a higher grade.
Does 93 octane give more power?
Premium gas must be, by law, at or above 91 percent, although you do also see 93 percent octane ultra-premium at many stations. … Octane does not offer any better fuel mileage, increase engine horsepower, or make the engine start quicker. Higher octane only reduces the likelihood of engine knock or ping.
Does 98 octane clean the engine?
Octane is the measure of a fuel’s ability to resist the phenomenon known as ‘knocking’, not its cleaning ability. Knocking is the uncontrolled combustion of fuel that can destroy engine internals.
Can octane booster damage an engine?
A lower octane fuel than what is recommended can damage the engine as it causes ‘knocks’ or irregular ignition. … High octane fuel and boosters have negligible or absolutely no effect on low performance road-going cars. A manufacturer recommends a minimum octane fuel rating and these products will not make a difference.
Is Shell gas Really Better?
Gas Test Findings The results showed that on average, Top Tier gasoline had 19 times fewer carbon deposits on injectors, intake valves, and in the combustion chamber when compared to regular gasoline. … Variation in the results is attributed to the detergents used by different brands.
Does premium gas give better mileage?
Premium gas gives you more miles per gallon than regular gas. … In actual fact, you’ll get a greater range of fuel economy between different brands of regular gas, than you will between the same manufacturer’s regular and premium gasses.
How much HP does 93 octane tune add?
When switching from a 91 octane tune to a 93 octane tune you can expect to see an increase of 5 to 6 more horsepower on top of the gains you are already getting from an 87 octane Bama tune.
Does premium gas last longer?
Sadly, there’s nothing in premium gasoline that would make it last longer than other fuels from the pump. Since the distinguishing feature is the higher-octane levels, the only real benefit you gain is lowering the chance of engine knocking, which isn’t much of a threat on most modern fuel systems.
Who has the best 93 octane gas?
Costco 93Costco 93, it’s top tier or BP.
Can you run 87 octane on a 93 octane tune?
87 is the standard octane rating that most car engines are designed for. … The reason why you should never run a lower octane fuel on a vehicle tuned for higher octane fuel is because the tune has no way of detecting what fuel you are using and will continue to operate assuming you put in the correct fuel.
What gas is better 91 or 93?
Regular gas is rated at 87 octane in most states, while premium gas is often rated higher at 91 or 93. Fuel with a higher octane rating can stand up to higher compression before it detonates. Essentially, the higher the octane rating, the lower the likelihood that detonation happens at the wrong time.
Does 93 octane give better mpg?
At triple-digit speeds, the higher power on 93 octane gave the Charger an advantage measured in tenths of a second. The Dodge also posted a 0.3-mpg improvement on premium with its average of 23.5 mpg.
Can high octane fuel damage an engine?
The higher octane gives premium gas greater resistance to early fuel ignition, which can result in potential damage, sometimes accompanied by audible engine knocking or pinging. … But if the vehicle manufacturer says your engine needs only 87-octane regular, that is what you should use.


text:
New Delhi: Travel And hospitality solutions provider RateGain Travel Technologies made a weak initial public offering (IPO) debut on the exchanges on Friday.
The shares the company got listed at Rs 360 on the NSE index, a 15.29 per cent discount over its issue price of Rs 425. On the BSE, the scrip started trading at Rs 364.80.
The Software as a Service (SaaS) company’s initial share sale saw a strong response from investors, with the issue subscribed 17.41 times on the last day of the offer on December 9.
According to the data, non-institutional investors had bought shares 42.04 times the portion set aside for them and the qualified institutional buyers’ portion was subscribed 8.42 times. Retail investors had put in bids for 8.08 times the allotted quota and the employee portion was subscribed 1.37 times.
All the categories, including those for Qualified Institutional Buyers (QIBs) and Retail Individual Investors (RIIs), were oversubscribed.
According to the data, the portion for QIBs was subscribed 8.42 times
The company had raised Rs 599 crore from anchor investors. The travel and hospitality technology services provider’s offer is expected to fetch Rs 1,335.73 crore at the upper end of the price band.
Among other purposes, proceeds from the fresh issue will be used for payment of debt availed by RateGain UK, one of the subsidiaries, from Silicon Valley Bank; payment of deferred consideration for the acquisition of DHISCO and strategic investments, acquisitions, and inorganic growth.
It will also utilise fresh issue proceeds for strategic investments, investment in technology innovation, artificial intelligence and other organic growth initiatives, and purchase of certain capital equipment for data centre.
The company will repay debts availed by RateGain UK, one of the subsidiaries from Silicon Valley Bank, and make a payment of deferred consideration for acquisition of DHISCO.
The financial result of the firm shows that losses of RateGain widened to Rs 28.57 crore in FY21 from Rs 20.1 crore a year ago because of the Covid-19 crisis. Revenue from operations declined to Rs 250.79 crore from Rs 398.7 crore during the year.
ALSO READ | Opening Bell: Sensex Tanks Over 500 Points In Early Trade, Nifty Close To 17,046
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domain: economics


“Use real identities to reduce abuse online” is a talking point you’ve almost certainly seen down the years. It also seems to come around like clockwork every other month, and is currently a hot topic in the UK after prominent journalists / media personalities raised the issue.
It’s an interesting idea, but the devil is in the details. “Verified identities solve the problem” won’t address the new problems such an approach creates. Is it possible to make this work, or is it all just pie in the sky?
Real users still behave badly
Think back to some of the worst arguments you’ve seen on social media. They almost certainly involve verified accounts somewhere in the mix. Often they initiate the aggression, or wade into replies and make it worse.
They may also utilise platform features to spread the argument further afield. Accounts with large followings on Twitter will do this via quote tweeting. They may simply retweet a stance they disagree with to initiate a so-called “pile on“, or retweet other people arguing, or quote tweet adding their own commentary along the way. They may even retweet their own replies.
Once this happens, it’s often game over for the other person whose notifications are essentially ruined with a flood of angry responses. I could be wrong, but I don’t believe I’ve ever seen a verified account banned for causing a pile on. I have, however, seen small accounts targeted by such things delete their profile completely. On balance, this doesn’t seem particularly fair.
Realness doesn’t equate to accuracy
Going back to Twitter, this is somewhat a problem of their own making. Whether an accurate assumption or not, the verified system was originally where you assumed all the celebrities you liked ended up. Twitter expanded it to include other people of note, for example authors, athletes, scientists, and so on. Then lots of folks were handed verification simply for working in news / media orgs. Alongside this, for a period of time you could submit a request to be verified and if you passed the bar, you got your checkmark.
Already, you can see how the system was torn between notions of “Is this a badge of notability, identity, or something else altogether?” Things became even more confusing as for a few years, the Twitter verification information page insisted verification was not currently happening…while new checkmarks continued to be given out.
The scheme is currently undergoing renovation, but it remains to be seen what happens with it.
Whether intentional or not, people seem to trust verified accounts as trustworthy voices of reason. This is not sensible, as people will tweet whatever they feel like. If we’re asking, “Does verification help reduce abuse or misinformation”, it can be argued that no, it does not. A drop of 73% in election misinformation after Twitter suspended Donald Trump is a frankly staggering statistic.
This alone should be a fatal blow to the “Use a real identity and things will magically be better somehow” idea.
Facebook’s foray into real names
Facebook already requires you to register an account with your legal name. The problem is if they think your name is not real, you’re locked out and have to try and regain access. This has had very mixed results, causing problems over everything from “fake” names to Star Wars.
Consider all the effort involved in policing this, and the hassle for site users, and then compare that with the number of accounts who are happily pushing large-scale propaganda campaigns via fake profiles on Facebook anyway.
Is it really worth all that effort? Is it helping?
If we want everyone online with a real ID, there’s many privacy issues up for debate if identity documents are involved. There’s also the massive problem of access. The international gold-standard for ID is your passport. Many verification schemes ask for scans of your passport at some point.
Problem: lots of people don’t have passports, because it’s not a mandatory document. Depending on country, it might be very expensive. It could involve a complicated process or have its own barriers to entry. Live in a different country to the one you were born in? You may only have a residence permit. It’s possible your passport has expired. Will they even accept an expired passport?
In 2018, around 76% of people had a passport in the UK. That compares with 42% of Americans and 66% of Canadians. That leaves an awful lot of people out of the loop across just 3 locations. This is before you factor the rest of the world in.
Unless passports are somehow made free worldwide, or a universal form of ID is created, people will lose out. When crucial services like banking, tax, municipal services, gas and electricity are all moved online, this seems irresponsible. We typically don’t need to show our energy company a scan of our passport to use their service online. Does it make sense that the bar to entry is so much higher to post on a social network?
There are limited circumstances where a social network currently may ask to see a form of identification. That’s mostly tied to issues of death and memorialisation. Similarly, some verification processes involve passport scans.
Scanning everybody, though? That’s going to cause additional problems…
All the eggs, in the biggest basket
Any social media app containing something approaching the whole world’s passports is instantly a massive target for hacks and scams. It’s debatable if they could keep it all secure and locked down—they only have to fail once. For comparison, the UK’s Home Office deals with a frankly unimaginable volume of personal data. Passports, birth certificates, wedding certificates, photographs, personal emails, biometrics, the works. Some of this is outsourced to third parties.
It is incredibly important this data is kept under lock and key. This is now the point where we mention a 120% rise in data loss incidents. With 4,204 incidents “in the last financial year” alone, that’s an awful lot of problems related to paper documents and electronic devices. If this is the scale of the issue for UKGOV despite their best efforts, imagine the problem for a much less wealthy social media site. It just seems too much of a leap of faith to think this would end in anything but disaster.
This leads us neatly on to…
Data theft fallout
When people say that losing their anonymity online “isn’t a problem”, or “wouldn’t bug me”, that’s great for them. But just because something isn’t in their threat model, doesn’t mean it can’t hurt someone else, as the EFF’s Eva Galperin pointed out on Twitter only recently:
Some people are at risk from domestic violence or racial abuse. For some, anonymity is built into aspects of their job. For others, their stay in a country might be. conditional but they’d like to speak up on the issues affecting them without feeling they’re jeopardising their status.
“You’re not living in a repressive regime” should not be the barrier to entry for privacy. Treating your right to keep yourself safe from data abuse isn’t a special exemption, kept out of reach except in the direst of emergencies. This normalises the idea of privacy and safety as an exception. You know who loves it when privacy and safety are treated as abnormal?
People who’d rather you have as little of it as possible, that’s who.
Same again next time?
I’ve seen this discussion come around many, many times now. No matter the circumstance, it tends to fizzle out and be resurrected a few months later. In the UK, at least, “everyone should supply ID” will collapse under weight of sheer impossibility. The task there is made harder by virtue of the fact there is no nationally issued, mandatory identity card system in operation.
Things are a little more complicated in the US, where anonymous online speech is concerned. The legal provision that protects free speech online—Section 230—is under increasing scrutiny. It remains to be seen how things will play out there.
Having said that, this talking point will return. When it does, you’ll be armed with the knowledge that data privacy is incredibly important. Due to a variety of social, legal, and practical problems in this particular realm, social media sites won’t be asking you for verification any time soon.


text:
Welcome to the Week in Review.
1. Patients Urge Senators To Curb Patent Abuses📝
- Following last week’s six figure ad campaign launch, P4ADNow is continuing to push the Senate to pass a bipartisan package of bills to curb Big Pharma patent abuses and ensure competition to lower drug prices. As part of continued grassroots advocacy efforts in ten states, Jacquie Persson, a patient advocate from Iowa, shared her experience living with Crohn’s disease and explained how Big Pharma makes enormous profits from her prescribed medication, Stelara, by gaming the patent system. Johnson & Johnson is able to “make an additional $18 million” per day in revenue from Stelara’s prolonged market exclusivity in the United States through the use of patent thickets, Jacquie says in the video. Jacquie is one of many patients who have experienced unjustified high prescription drug prices because of Big Pharma’s patent abuse. As P4ADNow’s David Mitchell points out, the good news is that “there is a real opportunity to advance common sense solutions supported by both Republicans and Democrats in the Senate that will deliver relief to patients through lower drug prices,” reported Inside Health Policy this week. Reforms that address patent and regulatory abuses and allow generic competition to come to market sooner have been long-sought by patient advocates — time to get it done, Senators. — (P4ADNow, P4ADNow, Inside Health Policy)
2. Defending Medicare Negotiation
- While big drug companies are throwing in the kitchen sink to try to stop Medicare negotiation, patients, advocates, and lawmakers are holding their ground in defending the historic law. This week, P4ADNow joined Public Citizen in filing an amicus brief in support of the Department of Health and Human Service (HHS)’s opposition to Merck’s lawsuit against the Medicare negotiation program. The brief explains how Merck’s patent abuses unfairly drive up drug prices for patients and calls out its false claims that the Medicare negotiation law is unconstitutional. Merck’s lawsuit would hurt patients like Steven Hadfield, an advocate who takes Merck’s expensive biologic drug Januvia to manage his type 2 diabetes. On the Senate floor, Majority Leader Chuck Schumer and Senator Amy Klobuchar fiercely defended the Medicare negotiation law. Several Members of Congress followed suit — Rep. Ro Khanna shamed Johnson & Johnson for price gouging its drug Imbruvica; Senator Ron Wyden explained how millions of patients will feel relief from negotiated drugs; Senator Cortez Masto highlighted the savings expected for Nevadans from negotiation; and Senator Peter Welch pushed back on Big Pharma lies about the new law curbing drug innovation. P4AD’s David Mitchell joined Bloomberg’s podcast “On The Merits” to discuss the urgent need for negotiated drug prices, especially for cancer patients facing huge costs like himself. “The drugs I take carry a list price of about $960,000 a year, just one of those drugs … cost me more than $17,000 out-of-pocket annually,” David explained. “I’m a lucky guy, I’m alive, I’m grateful, but the drugs I’m taking are way overpriced.” — (Public Citizen, Fierce Pharma, The Hill, Common Dreams, KTVZ, Office of Senator Cortez Masto, WCAX, Bloomberg Law))
3. The Inflation Reduction Act: A Clear Win For Patients
- While the historic Medicare negotiation program is making headlines, patients are already saving on drug costs because of the other provisions in the Inflation Reduction Act. Reforms such as: capping the monthly cost of insulin prescriptions to $35, making vaccines free, capping out-of-pocket drug costs to $2,000 in 2025, and penalizing drug companies that raise their prices faster than inflation. The new law is already providing relief to 27,000 Minnesotanswho will benefit from the $35 monthly insulin copay cap. Quinn, a patient advocate living with type 1 diabetes, wrote in an op-ed that the new law not only delivers cost savings to patients on Medicare, but assures peace of mind knowing that access to life saving medications is being expanded. Patients on Medicare have been forced to pay unjustified high prices set by drug companies for years. “In the past year and a half, our prescriptions have skyrocketed from $100 monthly to between $350 and $450, leaving us to make tough financial decisions,” wrote New Yorker patient Dawn in an op-ed. “I am one of the people who will save hundreds of dollars each month thanks to this historic announcement.” — (Minnesota Reformer, Syracuse)
One more thing: A new contract between drug company Regeneron and HHS marks “the first time the Biden administration has directly used its leverage to challenge drugmakers’ list prices.” Rachel Cohrs at STAT has the breakdown.
Have a great weekend!


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text:
The Batavia is probably one of the most inspiring ships built in modern history.
Reconstructed without any plans available.
Master Shipwright Willem Vos started his research to build the Batavia in the 1970’s .
He studied old paintings and stock-lists, all around Europe, he visited museums alike .
After his research was completed he made hundreds of detailed sketches of all parts of the ship. With those the reconstruction of the Batavia began.
The construction plans included in this book were made AFTER the Batavia was completed !
May you enjoy the images and drawings
as much as I did enjoy photographing
and designing the books about this unique ship .
The Last East Indiaman - Batavia
(PS: Owners of this book will receive a discount on the digital files of the full size scans of the construction plans as available from www.bataviaphotos.com )

domain: artistic


Over The Fence
An observant Curtis Park resident was briskly walking the track around William Curtis Park when she witnessed a man backing his Ford Ranger up to a huge pile of shredded bark meant for the children’s playground. She thought to herself, “What a shame to steal from the City and the children of our community.”
She dialed 9-11 and reported the alleged thievery to police. My first thought was, why is there a mountain of bark dumped in the middle of the park? What would Leslie Knope (the main character of Parks and Recreation) do?
I called the City Parks And Recreation department and spoke to Lori Harder. She mentioned that the Sierra Curtis Neighborhood Association has officially adopted William Curtis Park through the City of Sacramento Partners in Parks program and it was part of the April 6th “Clean Up Day” at the park.
I then asked Terri Shuttle, who is the executive director of the SCNA, if she knew anything about the Curtis Bark Pile and she mentioned it was indeed part of a neighborhood cleanup day. They had requested bark for various spots; unfortunately, they were unable to bring the bark until a week later when no volunteers were readily available.
Soooo, one of the board members took it upon himself to work in the park one day to spread the bark using his pickup truck. Good for him! That’s when the police showed up. Oops. Turns out the Curtis Park resident who initially called the cops was barking up the wrong tree. Turns out the board member was just being a good deed doer. Now he is hesitant to go back and finish the job. The SCNA is looking at schedules to line up volunteers to take care of the rest of the pile but no date has been set.
Last month there was another episode of Yard Crashers direct from Hollywood Park. The lucky couple who received the grand makeover, “A Taste Of Tuscany,“ had a viewing party at Broderick’s in West Sacramento. For those who don’t know, “Yard Crashers” is a backyard improvement show where host Matt Blashaw surprises shoppers at Lowe’s, Green Acres, Emigh Hardware and other home improvement and garden stores with promises of an amazing backyard transformation. From drab to fab!
A lot of the episodes have been filmed in our own backyard in Sacramento. The Yard Crashers crew invades yards with jackhammers, cement trucks, shovels, shears and chainsaws. Before you know it they’re digging up turf, trees, shrubs and removing the greenery that has no purpose and replacing it with thoughtful landscape design.
There have been at least five episodes of Yard Crashers filmed in the middle class neighborhood of Hollywood Park alone in the last few years. Why Hollywood Park? Perhaps it’s because it has Hollywood in the name. Showbiz! Or it could be that the neighborhood has the perfect palette for the backyard makeovers.
The production company, Big Table Media, is actually based out of Sacramento. So it’s a short commute.
The show likes people who are showbiz ready, have a personality, and aren’t afraid to get their fingernails dirty. Also, have a backyard that needs some drastic improvements. On the show’s website they accept viewer submissions. Include a picture of you, your pathetic backyard, and why your yard deserves to be crashed. That’s a route you can take if you’re interested in getting a backyard makeover and want to be the envy of your entire neighborhood. The show airs on the DIY network as well as HGTV. The website for submissions is at www.Diynetwork.com
Last month I wrote about how Raley’s, which purchased the former Capital Nursery lot on Freeport Blvd, has no official plans for it…yet. There have been blue prints created and lots of rumors bandied about. Raley’s official spokesperson stated last month they have “no immediate plans for the site.”
I recently received an email from an “Over The Fence” reader with a great suggestion for the abandoned site. Phil Shroeder mentioned he thinks the property would be a good location for Beer and Food Festivals. “It would be ideal for a Sacramento Oktoberfest. High school musicians could be taught to play appropriate fest music and vendors could supply food and beer. The bus lines are right outside and the police are right down the street. It is just a dream I have of being able to hold European festivals right here in good ol’ Sacramento.”
I like the way Phil thinks! Sounds like a great idea. Throw in some lederhosen and dirndls and it could be a beer and sausage party on Freeport!
I have my own dream for that empty lot. I’d love to see somebody bring “The Zombie Hut” back to Freeport Blvd. “Delightfully different dining with a Polynesian atmosphere.” Oh, and luscious fruity island cocktails topped off with cheery miniature umbrellas. It would be the pride of the neighborhood!
Readers: What would YOU like to see at the former Capital Nursery spot? Send me your ideas at Greg@Valcomnews.com and I’ll publish them in next month’s column. Also, if you have any local neighborhood gossip or items of interest, feel free to send me an email at Greg@valcomnews.com


text:
Here is a very yummy cake i made for Dena, requested by her Husband for her Birthday/Mothers Day. But Shame on me I didn't check very very carefully and just notice yesterday that i spelled Birthday with out the H on the tag :( Well you live and learn right. I made a deep chocolate cake with premium quality chocolate and made chocolate Mousse which took 1 hour to make, 45 of those minutes were spent constantly stirring the egg yolks and milk on low heat until they boiled, but it was worth it, the finished product was AMAZING so light and very rich with out much sweetness. I had great fun making it.
I love my world to be sweet and to share the goodness of that with other people. I am slowly inching my way into the baking and cake decorating arena, and so far LOVING it. I use my friends and family as test subject and have not had any casualties but rather very happy success. I am a family women with an amazing and supportive husband and 2 adorable, adventurous boys. I live to see people smile and when i see that i have done that when i delivery a special sweet treat to someone, i know I am on the right track.

domain: food


text:
1 week free rent!!!3 air-cons cover the whole unit, Large Balconey
As NEW, superbly designed 2 Bedroom 2 bathroom apartment situated on one of the highest points in Chermside, short walk to Westfield BUT far enough away from the hustle and bustle. 1-week free rent with a 12-month lease.
Special features of this special Apartment include the following: • Best location of the building • City and surroundings view from wrap around balcony. • 3-Air-conditioned BOTH
First listed on 20 June, this unit has been on Domain for 29 days (last updated on 20 June).
81 other 2 bedroom unit in Chermside have recently been sold.
枫林阁 Landmark Restaurant
Gympie Rd & Hamilton Rd, Chermside, BrisbaneQLD4032
Shop 1 – R64/65 Zone A, Level 2 Westfield Shopping Centre Cnr Gympie & Hamilton Rds Chermside QLD 4032, BrisbaneQLD4032
Pho Vietnamese Kitchen
Gympie road, BrisbaneQLD4032
Shop R63 Westfield Chermside, BrisbaneQLD4032
Kamikaze Teppanyaki Chermside
Westfield Chermside, Corner Gympie Road & Hamilton Road, BrisbaneQLD4032
The Sporting Globe Chermside
895 Gympie Road, ChermsideQLD4032
Tapworks Bar & Grill
Westfield Chermside, Cnr Gympie & Hamilton Rds, ChermsideQLD4032
Kedron-Wavell Services Club Inc.
21 Kittyhawk Drive, ChermsideQLD4032
School Catchment Zones for 59 Latham St LATHAM STREET


text:
Although the smart home is convenient, many people do not customize the password when installing it, so hackers can control the victim’s system by using the preset password. The United Kingdom recently introduced new regulations that require manufacturers to use unique codes instead of common preset passwords.
The UK’s newly introduced “Product Security and Telecommunications Infrastructure Act” aims to improve the security of smart home devices, prohibiting manufacturers from using common preset passwords and requiring disclosure of the release date of security updates. Violators will face hefty fines. This regulation began to be consulted last year and is now finally implemented. Violation of the new regulations can be fined up to 10 million pounds or 4% of the company’s total revenue. The law applies not only to manufacturers, but also to companies importing technology products into the UK. The products covered include smart phones, routers, security camcorders, game consoles and speakers, as well as electrical appliances and toys that can be connected to the Internet.
The British government stated that there have been 1.5 billion attempts to attack IoT devices in the first half of 2020, and the consequences can be serious. For example, in the 2017 attack, hackers attacked an Internet-connected fish tank from a casino. The data was stolen.
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domain: law


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The purpose of this study was to determine the capacity of Niemann-Pick type C (NPC) fibroblasts to transport cholesterol from the cell surface to intracellular membranes. This is relevant in light of the observations that NPC cells display a sluggish metabolism of LDL-derived cholesterol, a phenomenon which could be explained by a defective intracellular transport of cholesterol. Treatment of NPC cells for 4 h with 0.1 mg/ml of LDL failed to increase the incorporation of [14C]oleic acid into cholesterol [14C]oleate, an observation consistent with previous reports on this cell type (Pentchev et al. (1985) Proc. Natl. Acad. Sci. USA 82, 8247). Normal fibroblasts, however, displayed the classical upregulation (6-fold over control) of the endogenous esterification reaction in response to LDL exposure. Incubation of normal or NPC fibroblasts with sphingomyelinase (100 mU/ml; Staphylococcus aureus) led to a rapid and marked increase (9- and 10-fold for normal and NPC fibroblasts, respectively, after 4 h) in the esterification of plasma-membrane-derived [3H]cholesterol suggesting that sphingomyelin degradation forced a net transfer of cholesterol from the cell surface to the endoplasmic reticulum. The similar response in normal and mutant fibroblasts to the degradation of sphingomyelin suggests that plasma membrane cholesterol can be transported into the substrate pool of ACAT to about the same extent in these two cell types. Degradation of cell sphingomyelin in NPC fibroblasts also resulted in the movement of 20-25% of the cellular cholesterol from a cholesterol oxidase susceptible pool into oxidase-resistant pools, implying that a substantial amount of plasma membrane cholesterol was internalized after sphingomyelin degradation. This cholesterol internalization was not accompanied by an increased rate of membrane internalization, as measured by [3H]sucrose uptake. Although NPC cells showed a relative accumulation of unesterified cholesterol and a sluggish esterification of LDL-derived cholesterol when exposed to LDL, these cells responded like normal fibroblasts with regard to their capacity to transport cholesterol from the cell surface into intracellular sites in response to sphingomyelin degradation. It therefore appears that NPC cells, in contrast to the impaired intracellular movement of lipoprotein-derived cholesterol, do not display a general impairment of cholesterol transport between the cell surface and the intracellular regulatory pool of cholesterol.
|Journal||BBA - Biochimica et Biophysica Acta|
|Publication status||Published - 1989|
|MoE publication type||A1 Journal article-refereed|


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“Everyone’s got a theory how it started, about Castle Rock’s original sin,” a narrator says in Hulu’s teaser that hones in on the small town that serves as the setting for the psychological horror series from Stephen King and J.J. Abrams.
“Whoever’s sin we’re paying for, we’re trapped in a cycle that stretches back centuries,” the voice says. “There’s blood in every backyard ... inside every house. People say it wasn’t me … it was this place. And the thing is, they’re right.”
Castle Rock is an original story that takes place in King’s multiverse and combines the mythological scale and intimate character storytelling of King’s best-loved works, weaving an epic saga of darkness and light, played out on a few square miles of Maine woodland.
The series stars André Holland, Melanie Lynskey, Sissy Spacek, Billy Skarsgård, Jane Levy and Scott Glenn.
Sam Shaw and Dustin Thomason developed the project for television and serve as executive producers along with Abrams, Ben Stephenson and Liz Glotzer. It is from Bad Robot Productions in association with Warner Bros. Television.
Castle Rock premieres July 25 with the first three episodes launching on Hulu.


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District Attorney Bill Adair said the charges stem from abuse suffered between 2004 and 2009 at Wade’s home. The case was indicted in 2010 by a Walker County grand jury.
Wade took the stand during the trial, as did his victim, the victim’s mother, then-investigator for the District Attorney’s Office John Rowe and Debbie Wilbourn, a forensic interviewer from the Prescott House in Birmingham.
Adair said the testimony of Wilbourn regarding the victim interview at the child advocacy center was important to securing the conviction but was almost excluded because of issues getting the expert to court. Adair said he is grateful to the Prescott House for its assistance in cases, but this case highlights the reasons he believes it is time for Walker County to start its own center.
“This case illustrates the need for a CAC in this county,” Adair said. “This is the kind of case, with a local child and events happening in our community, that we will be dealing with on a day-to-day basis.”
In addition to conducting the interviews in a more timely manner, he said it would also ensure that the interviewers were readily available for court.
“One of the tragedies I see is that we have far too many of these cases,” Adair said. “The need is just so great.”
The Walker County Children’s Advocacy Center has been one of Adair’s top priorities since he was elected. The center recently took the step of purchasing a building and hopes to be fully up and running very soon. The center has received a great deal of community support in the form of monetary and materials donation to get the center started.
Although Wade had been out on bail pending the resolution of the trial, he was handcuffed immediately following the verdict and taken to the Walker County Jail to await sentencing.
The sodomy charge is a Class A felony with a possible sentence of 10 years to life in prison. The sex abuse charges are Class B felonies and carry a possible sentence of two to 20 years each. If Wade is ever released from prison, he would be required to register as a sex offender for the remainder of his life.
The defense in this case has asked for a pre-sentencing report from the state’s Pardon and Parole Board. This is a written report with detailed background information on the individual, including any prior convictions. Adair said he hopes sentencing will take place within the next 30 days.
Adair said his office is asking the judge for a tough sentence because of the nature of the crime. “We believe this kind of case requires a stiff penalty, and we will be asking for a harsh sentence from the judge,” Adair said.
This was the second jury trial in the county this week for the District Attorney’s Office, and Adair expressed appreciation to his staff, the jurors and the judges for their assistance in clearing up the older cases.
“I very much appreciate these judges pushing through these cases,” Adair said. “And I appreciate the jurors that had to sit through the trials. I know it is a great inconvenience to people to have to take time out of their lives to serve on a jury, but it is so helpful in trying to get the backlogged cases cleared.”


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Gibson’s collaboration with Nick Lucas gave birth to a majestic and powerful model. The deep body provides a full bass tonal range with crisp and lively treble tones. The sound is remarkably balanced across every register. This Limited Edition features beautiful Mystic rosewood back and sides and a Sitka spruce top. Classic Nick Lucas MOP inlay, Gibson Script logo, and Fleur De Lis detail this highly custom model. Finished with nickel open back tuners and Vintage Sunburst finish. Upgraded with an LR Baggs VTC pickup for easy plug and play anywhere you perform. Ships with a Certificate of Authenticity.
Gibson LE Nick Lucas Acoustic Electric Mystic Rosewood Features
- Stunning Mystic rosewood back and sides
- 12 fret neck-to-body joint to superior tone
- Nickel open back tuners
- LR Baggs Element VTC pickup

domain: design


text:
- Age / Gender:
- 24, Male
- Inside your Head
- All Stats >
Well, as you may not know my name is Ryan. And I'm guessing if your reading this, you'll want to know more about me? Very well. Erm, I am a social character at times, I'm a Gamer, I enjoy watching films and listening to music and I love drawing.
- Community Stats
Level 9 Blank Slate
Ranked as Scout
Contact Info / Websites
2009-04-08 06:49:06 by raz183
Sounds rather ambitious doesn't it? Well this is my next project. It's going to be hard, of course, but if you haven't noticed, I haven't been submitting many submissions to Newgrounds lately. That's because I have been perfecting my art skills and learning actionscipt 2 (I really can't be arsed with AS3 as of yet, but AS3 will be the coding most likely for MvCxK).
I wouldn't mind help with this project, I've done some "Concept Drawings" (aka Rotoscoping single-frames of animation for characters that may be used) so people can get a taste of what's to come.
I'm not aware of any 3 v.s 3 fighting games made in Flash yet, so this should be interesting. An additional feature I'm looking into is the possibility of using a game pad/ PC fighting stick instead of only just a keyboard.
So you see, looking at that, there's quite a lot of things already. Any help will be greatly appreciated, or if you would like to collaborate with myself (and hopefully others) to make this project a reality, please just message me or leave me a comment and I'll get back to you as soon as possible.
If you're thinking that you're unsure if i'd finish Marvel vs Capcom x Konami, I'd be glad to prove you wrong. Why, you ask? Because I grew up with such greats like MvC, street fighter, MSH v SF .etc, So I feel like I'm paying homage to my gaming past making this project.
I'm guessing you'll want to know characters as well? Well, as it is a Flash game, I was thinking 3 each company, so that would be 3 Marvel, 3 Capcom and 3 Konami. The characters at the moment seem to be:
Hulk (Large of the Group)
(Any "Large" character)
Triangle-Head (Large of the Group)
I'll be using the original sprites from Marvel vs Capcom (Where applible) as a rotoscoping "Baby Step", which means, I am thinking of creating a working prototype before starting the art (Rotoscoping) transformation.
Thanks for reading this, and if you want to help, just drop a comment!
EDIT : Sorry about Picture Quality, had to change File-Type in Flash and NG wouldn't take the .jpg format image.


GTX 1080 GPU: The New Gaming Beast: After a long time Nvidia finally announces it’s next generation high-end graphics card for Gaming and a VR. It about two year after Nvidia released it’s High-End graphics card after GTX 980 which is the best graphics card till now the next-generation GeForce GTX 1080 and GTX 1070 GPUs which have many new feature and have more edge to create in market and get ahead of there competitor AMD. Nvidia uses new Pascal architecture with these new graphics card, which creates giant leaps of performance and power efficiency as compared with previous generation GTX 980 graphics and also Titan X GPU which is cost about $1000.
Release Date and Price:
- Nvidia announces that the GPU will be shipped worldwide on May 27 with a price of $599 for GTX 1080 and for GTX 1070 will be shipped on June 10 with a price of. GPU also available “founders edition” as they were overclocked which gives maximum performance out of this beast and cost about $699 for 1080 and $449 for 1070.
Nvidia held the event in Austin in the Dreamhack festival as Nvidia CEO Jen-Hsun Huang introduced these two amazing products with nice keynote. Huang telling about the performance of GTX 1080 is twice as powerful and thrice as power as compared to last offering GTX Titan X GPU, while it is faster than two GeForce GTX 980 cards in SLI. The other 1070 is also powerful than the Titan X but is not talk much by Nvidia CEO.
1. GTX 1080
- As we get know how this 10xx series perform this getting interesting to know that GTX 1080 can take about 9 Teraflops of single-precision graphics compute power which is awesome for any gamer it also include 8GB of VRAM which is enough to run any game and performs like a beast,Nvidia uses Micron‘s new, faster GDDR5X RAM running at 10Gbps for a total bandwidth of 320GBps which is enough to get performance out of it. It require 180W of supply, and Nvidia also make compatible with 500W power supply as adequate for a PC with a single one of these cards which won’t cost user to buy for more PSUs.
2. GTX 1070
- While the other one is rated for 6.5 Teraflops while it also include 8GB of VRAM, but the GDDR5 is normal one not like the GTX 1080 which is phenomenal performance. Both GPUs are themselves best in class and solve your purpose is gaming.
The others qualities are as follows
- Both have same capability including support for DirectX 12_1, OpenGL 4.5, Vulkan, and Nvidia’s own G-Sync, Game stream, and GPU Boost. These are the feature which are must for the games to get best in class performance.
- The card require a single 8-pin PCIe power connector which is common in today CPU. And As we talk about outputs it include DVI-I, one HDMI, and three Display Port outputs.
- In the Nvidia showcase of the GTX 1080 shows that it run at a clock speed of 2.1GHz on normal cooler at 67 degree Celsius which is good work in terms as Nvidia CEO has said they have achieved this type of performance with fan while they earlier don’t able to get this performance by liquid cooling. While other manufactures using Nvidia chipset set a fan over the GPU to cool easily.
- As Nvidia CEO has said they don’t several R&Ds and efforts of thousand people to get this Pascal GPU project and able to complete within a span of two years. Nvidia has spend several billion dollars for R&D of GTX 10xx series and the efforts bore fruits as they able to create best GPU in the market.Pascal is said as the most efficient architecture that Nvidia has ever created. It is going to hard over there competitor AMD as AMD also have some plan to get over this market and released its next generation architecture within this month. Nvidia has created world’s first 16nm FinFET GPU which is the GTX 1080, and also the first GPU to use new GDDR5 Micron memory the first to use Micron’s new GDDR5X memory.
User who and to enjoy artwork recent games and production value Nvidia announces Ansel World’s first in-game 3D camera system. This is very awesome technology allow user to adjust independently adjust camera positioning and angles to capture scenes within open game worlds. As for compatibility Nvidia include drives and requires an SDK within the game. This the best way to seeing the new world within the game and enjoy whole in way to interact with it user can capture frames which are otherwise not easily composed, by moving into areas which game characters ordinarily wouldn’t be able to reach. Images are exported at way higher resolution than the output to the monitor, making the technique superior to ordinary screenshots. They can be adjusted for colorization, tonality, vignetting, and other effects, and exported to photo editing software as raw data. User can get the benefit full 360-degree stereo which is phenomenal in sound, user can export this content to other VR in the market like HTC Vivbe or Google Cardboard box ,etc. Recently supported games are The Division, The Witness, Lawbreakers, The Witcher 3 Wild Hunt, Paragon, No Man’s Sky, and Unreal Tournament and expected more to come in the recent months.
It is Nvidia way to get VR in support as VR is now becoming common and games are best companion to use with it so Nvidia rolled out VRWorks. It is just toolkit for the VR content also include PhysX and VRWorks Audio. Physical modeling allows objects to behave content like original and also look real world surrounding like real-world and it also obey law of nature and feel realistic. VRWorks is also comes with Audio which is awesome technology and feels like you are in real world and its audio engine is best in class. There is also feature to interact allow user to physically interact with environment you’re in as you move through it sound are in many variation to feel like it is real.
* Multi Projection:
Last but not least Nvidia also include multi projection for user to view independent view ports. This feature allow user to use multiple flat monitors,while earlier user has get chopped images which looks annoying but instead of having one large frame on a single frame , each monitor can receive its own feed with the view port and perspective adjusted to its angle relative to the other monitors. Earlier this type of feature require to require three independent graphics cards, but can now be get with a single GTX 1080, which can output three different projections in a single pass. This also benefited VR which get projection for each eye without any other necessary hardware.
Last but not the least as Nvidia finally starting gaming in 4K which is one of superb feature we get in 1080 as player can et the advantage of new gamin resolution as 4K is till not available for gamers to play and 4K gaming may become mainstream. The current generation 4K supported by GTX 98x and Titan X GPUs.
- After a span of two years Nvidia finally listen to the gamers who wanted hard-core gaming and they released it’s new two next-generation GTX 10xx series which is the high-end and it is the best high-end GPU in the market as compared with the manufactures like AMD which also planned a event this month let keep our finger crossed and hope other technology with battling


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President Joe Biden is declaring that the risk of nuclear “Armageddon” is at the highest level since the 1962 Cuban Missile Crisis, as Russian officials speak of using tactical nuclear weapons after suffering massive setbacks in the eight-month invasion of Ukraine. Speaking at a Democratic fundraiser, Biden said Thursday night that Russian President Vladimir Putin is “a guy I know fairly well” and the Russian leader is “not joking when he talks about the use of tactical nuclear
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Power in the Fjords
When it comes to energy, Norway has long been regarded as one of Europe’s most self-sufficient nations. Besides an abundance of water and hydropower plants, it also has substantial resources in North Sea oil and gas. Yet, surprisingly, the country still imports large amounts of electricity each year – a situation that is soon about to change.
Norway is rapidly building new hydropower stations in a determined effort to reduce its dependency on imported electricity.
The country still has to import about 10 per cent of its electricity each year, despite its access to vast energy resources. However, a plan to become more self-sufficient by expanding its own hydropower network is well under way.
Epiroc operated under the trademark “Atlas Copco” prior to January 1, 2018.


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Flying RC has been pretty standard to use a radio transmitter (Tx as it is called, DJI really screwed it up when they started calling an RC Tx a "remote controller") Actually DJI got a bunch of things wrong with there designs and lingo. IMHO. If you ever consider flying RC in the future (fixed wing, quads, heli's) I think learning and using the standard equipment that you'll find at every RC field (prob. in the world) would be in the "best practices" area. I also think that stick resolution and accuracy would not be anywhere near using a normal Tx.
I too practice full scale on the sim and dont use a yoke, which I prob. should, but instead have used a stick with rudder pedals. But it seems interchangeable with flying full scale with a yoke and rudders.
That flight stick is a pretty interesting idea though!!!


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Superb very fast hybrid with a berry aroma and shiny crystals like diamonds, heavy glands, and a low structure, a fabulous branching pattern, ideal for indoor closets, is a good yielder, ready in 7 weeks.
Description by Motarebel:
Gryphon is our 1988 Ghash mother crossed to a lemon thai male from EGSC, The G-13 Hash plant mother has been a mainstay for us, great structure and powerful stone. Gryphon maintains that structure, the Lemon thai male adds some vigor and a powerful sativa stone. The male Lemon thai is from Dutchflowers via EGSC, it has a Hawaiian heritage. The smoke is like Lemon Larry with a diesel kick,very strong and balanced stone. Easy to grow and clone. Gryphon is an all around plant, very easy to grow Scrog style when you don't have enough heights to grow trees.
She responds well to any type of culture, we mostly grow soil using organics and encourage you to do the same in a effort to bring the best aroma to your meds.
Can be grown indoor & outdoor
Flowering time: 7-8 weeks / End of September
Indoor yield: Up to 450 gr / m2
Outdoor yield: Average of 300 gr outdoor or more depend on vegetative time and season, up to 450 gr
Lineage: Gryphon x Jack33
Bouquet: Very sweet berry floral flavor, peppery, rich and intense aroma

domain: agronomy