I let my friends check out my latest phone. They switched the ringtone from a song to a man shouting. They also turned the volume all of the way up. Someone rang me while I was driving. All of a sudden someone was shouting at me while I was driving. I didn't wreck, but it did take two or three minutes for my breathing to return to normal.
Sorrent announced a licensing agreement with Twentieth Century Fox to bring Fox film content to mobile phones. The first movie will be Robot. Sorrent will release a mobile game at the same time that Vivendi releases the console title. Sorrent and Fox will partner on other films, and the partnership will go beyond games to include wallpaper, ringtones, film clips, etc.
The question is: is this marketing for the film? content that should be sold separately? or both? I think the answer is eventually both, but should be advertising dollars in the near term. Today, it is content to be licensed - at least in this deal. Fox will collect a fee on each download associated with their films.
I think this deal will work for Sorrent. Sorrent produces wonderful mobile games (my favorite is Zuma) and is likely to generate a positive return on their investment even though film-based games have not been the top sellers that reach the casual game player. Carriers are eager for new titles and likely to help market them.
Will be interesting to watch in the long term, however, to see if selling this content is more beneficial to the film industry than giving it away as pure marketing.
I was browsing some of my vacation photos on Ofoto. As I looked through the album, I noticed a new feature on the side of the web page. It showed my photograph on a mobile phone. I gave it a shot - entered my phone number and carrier. No luck - my carrier not supported yet. No note setting expectations on when it will be available.
I think it's a sign of how nascent this market is and how low consumer expectations are. It's like turning back the clock to the early days of the Internet. People didn't mind so much if a link was broken - they just tried again another day. Guess I'll give it a shot in another few months.
Philadelphia's CIO (I didn't know cities had those) announced that Philadelphia would complete their ambitious plan to cover 135 square miles with Wi-Fi. The project will offer free Internet access in public parks and discounted rates for low-income residents.
While the plan is a noble one and will offer a plethora of benefits to the residents of Philadelphia, I think it falls short of fully "bridging the digital divide" - which does exist. According to Jupiter's numbers, about 2.5 million households with an annual income of $15,000 to $30,000 had broadband access in comparison to more than 12 million households earning in excess of $75,000 annually.
First, there is already a lot of free access in cities like Philadelphia and San Francisco. I can see nine access points from my flat, 43 at the nearest intersection, and three in the closest "garden" in Golden Gate Park near my home. Half of these are unencrypted.
Qualcomm which submitted one of four complete proposals for the 802.11n standard has decided to join the TGn Sync group giving more leverage to a group that already includes heavyweights such as Atheros, Intel, Nokia, Nortel, Panasonic, Philips, Samsung, Sanyo, Sharp, Sony and Toshiba. The question of whether the TGn Sync proposal or that of the Wwise group (Airgo, Bermai, Broadcom, Conexant, STMicroelectronics, and Texas Instruments) is technically better is open to debate with each proposal offering its advantages. The TGn Sync group, however, has lined up a number of consumer electronics companies on its side that strengthen their position. Streaming video over the wireless network will be one of the primary drivers of consumer upgrades to higher speed/higher capacity networks. Having the CE companies on their side early on, the TGn Sync group is well positioned to put forth a standard that takes into account stakeholders that are likely the largest consumers of Wi-Fi chips beyond basic home networking boxes. Adding Qualcomm to their side eliminates a detracting factor moving forward and will simply the process overall.
24 Hours - the thriller drama - aired it' fifth episode of the 2005 season last night. While just one of many similar episodes, it highlighted many of the reasons why a cell phone is not a good idea if you're a "bad guy" and brought ringtones to a prime time TV audience. A mother heard her recently murdered daughter's phone ring and instantly identified the ringtone as being unique to her daughter - one that her daughter had recently paid for and downloaded. While the "bad guys" initially covered it up by claiming that they too had the same ring tone, it's only a matter of time. Given the capabilities of CTU, it won't be long before they identify all of the wireless subscribers with that specific ringtone and realize that the murdered girl's phone was indeed on the premises.
Sprint has joined Verizon and Alltel in their decision at least to delay their decision to participate in the nationwide wireless 411 directory - a service which would allow consumers to opt-in to the service as opposed to paying to opt out like many landline services.
The service - to be offered by Qsent already faces a number of challenges beginning with the need for consumers to opt in rather than out and including recently passed legislation in California which requires the carriers to obtain written consent from the consumer before being allowed to include them in the list.
With ARPU's flat, carriers are certainly in need of incremental revenue opportunities, but I don't think they'll find a big win in wireless directory services. Cost is one hurdle, but consumer interest is an even bigger one. Mobile phone numbers remain "guarded" by many individuals - a piece of personal information to which individuals like to control access. JupiterResearch shows that consumers are interested in Directory Services (411) - in fact these services top the list of productivity services that interest consumers -, but they are primarily interested in pizza delivery, tow trucks, and taxi's - entities with landlines.
BMW has launched a promotional campaign on mobile phones testing a new medium, a medium to which many have access, but few - advertisers and consumers - use. There are nearly 160 million cellular subscribers in the U.S., but according to a recent JupiterResearch consumer survey, only six percent of online consumers have received an SMS from a business. Cellular phones are ubiquitous, but the majority of consumers are not comfortable with either the text messaging or WAP browsing features required to participate in this marketing campaign. BMW does indeed have a tech-savvy customer base more likely to understand how to download graphics to their phones, but this far from assures a great user experience.
With ALLTel's decision to purchase Western Wireless and their 1.4 million subscribers, they will move from a distant sixth place with about 8.5 million subscribers to 10 million subscribers - a less distant sixth (not considering that the Nextel and Sprint deal has not closed) from fifth place T-mobile with more than 16 million subscribers. This acquisition will move ALLTel from a regional player to a "leading" regional player position, but certainly not a national player given that they lack coverage in key urban markets.
The Western Wireless acquisition is certainly a fit in terms of geography adding customers in mostly regions in which ALLTel does not compete today. Operating statistics such as MOU and ARPU are similar for both companies, but low in comparison to national providers. Neither company was working on a 3G network which would enable new services. ALLTel investors rather than consumers are likely to see the near term benefits of this transaction.


TLC Integration understands that much of today’s technology causes strain to the majority of existing building power systems. Systems within businesses, plants, and schools are particularly susceptible . This strain causes equipment and circuitry failures such as corrupted data, poor security video pictures, and can damage other systems necessitating costly replacements.
We offer a wide range of power quality solutions for multiple power issues
TLC Integration can perform a thorough assessment of all possible power quality solutions factors based upon two elements. These elements are a flow chart of the electrical systems. This needs to be provided by the electrical engineer. We also need a one year’s power consumption record. This is usually provided by the building’s operations manager. TLC Integration Systems can identify and solve any disruptive issues within the power system, and savings can be seen quickly after installations have been made.
Some of these power quality issues that can arise are power failure, power surge, power sag, under-voltage, over-voltage, electrical line noise, frequency variation, switching transient, and harmonic distortion. It can be an individual problem, but most likely it is a combination of one or more of the above that causes many of a building’s problems. Power system and load incompatibility problems are common when the loads are nonlinear.
We have several case studies available that details our assessment of the building and the energy costs that were saved after installation.
TLC Integration Systems can provide any or all of the following:
- We offer a full review of your building’s system and energy costs
- We can design a new power quality system for a new building or existing facility
- We will offer money saving recommendations for your current system
- We can audit the power quality for any existing systems


text:
I gained a lot of personal perspective after many years of listening to my clients tell their stories. With every story I heard, I tried to take away something from the conversation. Something I could use in my own personal life and my own marriage. They say to learn from our own mistakes, but I think we can also learn from other’s mistakes too. Before I outline my marriage tips, I want to reiterate that I am not perfect. My husband is not perfect, and my marriage is not perfect. I do not always follow these tips. Marriage is work…hard work. Before you visit with a divorce attorney, consider these tips.
1. Communicate – Communicate about the biggest and smallest things. Tell your spouse about what happened in your day, and then ask him or her about their day. Don’t be afraid to ask for help. We might not ask for help because we are afraid the other person might view us as weak or that we can’t handle it, but couples are teams. When one teammate is down or needs help, the other teammate should step up and lift the other. Personally, I struggle with this tip. I often think I can handle it all, but I sometimes need help. If I would just ask my husband for help when I first needed it, I would not feel so angry or upset later. Communication is the key to a healthy marriage!
2. Intimacy – We all need physical and emotional intimacy with our partner. It doesn’t always have to be about the physical, the emotional intimacy is as much as important as the physical. Physical intimacy doesn’t have to be about intercourse. Just a hug or a kiss or holding hands. Don’t shut out your spouse and don’t give him or her the silent treatment. Don’t look to someone or something to fill the void. Again, if you feel the intimacy is lacking with your partner, communicate and tell him or her what you need.
3. Hobbies – Find something you and your spouse can do together. Such as golfing, or tennis, or fishing, or volunteering. Find something that is for you and spouse to do together. I know this is hard with work schedules and kid schedules, but when the children are grown and they don’t need as much attention as they do when they are younger, the couples need feel connected. Finding a common hobby or interest will help the couples to feel connected. Even if it is just playing video games together after the kids go to bed.
4. Try counseling – One of the first questions I ask during an initial consultation is “who is your therapist?” Too often the answer is “nobody” or “my spouse doesn’t believe in counseling.” Even if you don’t want to meet with a mental health professional, consider meeting with someone at church. I personally think all options should be considered before divorcing, including attending therapy. I have seen many marriages saved by marriage counseling.
5. Forgive – We are not perfect. Our marriages are not perfect. We all make mistakes. Sometimes the mistakes are too big to save the marriage, and in those instances consider forgiving the other spouse so you can move forward emotionally. There is so much strength in forgiveness. Bitterness kills the human spirit, and it kills the marriage. Forgive not for the other person, but for yourself.

domain: sociology


text:
The semester is finally over and the midyear vacation has begun! It’s been a while since my last getaway with my good friends and we’re really hype for new trips and breaths of fresh air. As for our first journey, we trekked to Mount Natib to hit two birds with just a stone! We went to see two amazing falls, a tall and a short one, mischievously connected by the same river.
Starting the hike
Our meeting place is at the Bataan Common Terminal, and from there, we have to ride a jeepney going to the town of Bagac with 35 pesos as fare. We left the terminal at exactly 10:30 am, as there is a 30 minute-interval in every trip to Bagac.
The jeepney dropped us off at Oximoso Street in Binukawan, Bagac as it is the road leading to the registration area for hikers who want to see the falls. There is a fee of 50 pesos, and I think it is for the conservation and protection of the area.
(Tip # 1: You may want to start your trip as EARLY as you can unlike us. We’re lucky because it’s a bit cloudy when we went there, it’s still hot, but not that much.)
The trail is a good 2 hours walk (we decided to walk because why not! HAHA). We started walking by 12:30 pm and mind you, it is hot! It is not advisable to bring a car (unless it is an off-road 4×4 one) because even if it is a concrete road, there are areas that are so steep. (but I think motorcycle will do)
Reaching Limutan Falls
Our lengthy march is indeed veryyyy tiring! We had some stop-overs and water breaks. And speaking of water,
(Tip # 2: Bring as much as water as you can. There is a hose of water at the river, and folks said that it is safe to drink. We fetched water from that hose and we even consumed it, only to find out that it has bits dirt and “tining”. For mineral water babies out there, I advise you to bring your own water bottle I mean BIG water bottle.)
Hiking is really exhausting, but upon seeing the marvelous Limutan Falls, all the fatigue and muscle pain paid off! We landed at the top of Limutan Falls, and we have to take an exorbitant side path in order to get down to the waterfall.
(Tip # 3: Path ways there are steep, I mean VERY steep! Wear shoes with a tight grip especially when it is raining, just be careful for you not to stumble and fall.)
The icy cold water from above is dropping benevolently over the naturally formed pool! It was not that big, but it’s definitely a cool place you should be planning to go to. Dipping in these waters relaxed my overworked leg muscles and took away the feeling of tiredness. Just beware of snakes and blood leeches or “limatik” on this spot.
The Way to Ambon-Ambon Falls
We still don’t want to leave but we have to because it’s getting late and we still have to catch another waterfall. The route that we took to Ambon-Ambon falls is so thrilling. From the pool of Limutan Falls, we walked through the boulders embedded directly on the river path.
(Tip # 4: The boulders are a bit slippery so be extra careful. There are many rocks behind and if you happen to slide and fall, it is really dangerous.)
There is a little waterfall before the Ambon-Ambon Falls, and we ended up being trapped there. We couldn’t find a path to go through it so we decided to go back and take the steep path at the riverside instead.
(Tip # 5: If it is your first time going to this place, make sure to bring a tour guide with you. You can hire a tour guide for 300 pesos at the registration area. There’s a big chance of getting lost if you really don’t have an idea where are you heading to. It just happened that my friend just went here years ago so we didn’t take a guide with us.)
Ambon-Ambon Falls’ Majestic View
Atlast! We reached Ambon-Ambon falls and it is really towering! Travel journals say that it is over a hundred feet tall, and there are big rocks at the bottom. There is also a pool before it, made by the little waterfall that put us into a dead-end. The view is so majestic! We could literally sit at the side of the falls (but I can’t because I’m afraid of heights). We didn’t have the chance to reach the bottom because folks say that the only way to reach it is through rappelling. It is really an amazing scenery!
Summing it up
The experience is indeed exhausting. But all the tiredness will be gone when you reach those falls! Just don’t leave trashes and do not alter some natural occurrences over the area to preserve and protect it.

domain: geography


Alex Chosen 26" Rear QR Quick Release Trail Enduro MTB Bike TR Wheel Black
Alex QR Quick Release 26" Rear Trail / Enduro MTB Wheel
Note: This is for a single wheel - pair shown for illustration only
- 26" version uses the Alex Volar 2.5 tubeless ready rim, 25mm internal & 30.2mm external widths - Creates a perfect tyre profile for tyre sizes of 2.35" or larger.
- Chosen Trail hub – Rear 135mm QR Quick Release, 4 sealed bearings, 32h, 9/10spd.
- 120 point engagement MGC freehub system allows for almost instant drivetrain engagement and an idividual freehub noise.
- ACI Alpina double butted black racing spokes. Strong yet light, stainless steel spoke with brass nipples.


The Heckscher–Ohlin model (H–O model) is a general equilibrium mathematical model of international trade, developed by Eli Heckscher and Bertil Ohlin at the Stockholm School of Economics. It builds on David Ricardo's theory of comparative advantage by predicting patterns of commerce and production based on the factor endowments of a trading region. The model essentially says that countries will export products that use their abundant and cheap factor(s) of production and import products that use the countries' scarce factor. Features of the model
Relative endowments of the factors of production (land, labor, and capital) determine a country's comparative advantage. Countries have comparative advantages in those goods for which the required factors of production are relatively abundant locally. This is because the profitability of goods is determined by input costs. Goods that require inputs that are locally abundant will be cheaper to produce than those goods that require inputs that are locally scarce. Theoretical assumptions
The original, 2x2x2 model was derived with restrictive assumptions, partly for the sake of mathematical simplicity. Some of these have been relaxed for the sake of development. These assumptions and developments are listed here. Both countries have identical production technology
This assumption means that producing the same output of either commodity could be done with the same level of capital and labour in either country. Actually, it would be inefficient to use the same balance in either country (because of the relative availability of either input factor) but, in principle this would be possible. Another way of saying this is that the per-capita productivity is the same in both countries in the same technology with identical amounts of capital. Production output is assumed to exhibit constant returns to scale In a simple model, both countries produces two commodities. Each commodities in turn is made using two factors of production....
Please join StudyMode to read the full document


text:
Brandon was the first patient to receive a cochlear implant at Saint John’s Health Center. Brandon was diagnosed with otosclerosis in his late 20s. Otosclerosis is a condition in which there is an abnormal growth inside the ear. Otosclerosis is a hereditary disease that can be passed down to generations. In his late 30s, there was a stapedectomy performed on each ear. About a year later, another stapedectomy was performed. Then after, he was diagnosed with profound hearing loss on both ears. Brandon did go back to his hearing aid.
When asked, what encouraged you to proceed with the cochlear implant? Brandon says,
“I knew my last chance was going to be a cochlear implant.”
Brandon did undergo (2) cochlear implant surgeries. However, Dr. Voelker was the revision surgeon for the second cochlear implant surgery and was able to successfully restore Brandon’s hearing. Brandon says,
“It was literally life changing… I could have not expected it to go this well. I feel like I’m getting the full benefit of the cochlear implant that I was originally expecting.”
Since the revision surgery, Brandon has been amazed with the progress in his hearing. He was worried he’d lose his hearing. Brandon says, “I don’t know what I would have done.”
A cochlear implant has many benefits such as improving hearing loss for patients of all ages and improve speech development. Pacific Neuroscience Institutes’ Cochlear Implant Program launched January 2022. The Cochlear Implant Programs mission is,
“Our community-based cochlear implant program strives to advance our patients’ quality of life, improve their ability to communicate with loved ones, and enhance their cognitive well-being. Our interdisciplinary cochlear implant team at PNI treats patients across the lifespan (from infancy to older adults) using evidenced-based practices, innovation, and a compassionate approach to care.” We’re happy to have had Brandon be our very first cochlear implant patient at Pacific Neuroscience Institute.
When Brandon was asked how his experience was working with Dr. Voelker and our team, he said,
“I was a special case because it was a lot more difficult. But even before it was a special case, I was treated like I was a special person. That’s one thing I will never forget.”
Learn more about Brandon’s story by listening to his Patient Testimonial.

domain: medical


There are some significant new capabilities in the latest version of both Simcenter MAGNET and Simcenter Motorsolve 2019.1. This includes a new electromagnetic and vibro-acoustics link among many others.
Predicting electromagnetic induced vibro-acoustics
In a faulty machine, the mechanical vibrations due to electromagnetic forces can be quite catastrophic. This can lead to damage, frequent servicing or total failure. The noise produced during operation can range from a minor irritation to a potential safety concern if too loud. As a result, it is important to perform linked electromagnetics and vibro-acoustics analysis early and at multiple stages of the design process.
To address this need, with both Simcenter MAGNET and Simcenter Motorsolve 2019.1, there are new options to account for the electromagnetically-induced acoustic noise and vibration.
The exported nodal force and force density fields from either software tool can be used as the load for structural and vibro-acoustic simulations, such as with Simcenter 3D.
Simcenter Motorsolve uses smart 2.5D technology to generate a 3D nodal force mesh model based on 2D simulations, significantly accelerating analysis with accurate results.
With Simcenter MAGNET, the 3D simulations allow for a detailed analysis of problems like power transformers and final design verification of electric machines.
New generalized electromagnetic force calculation
In Simcenter MAGNET 2019.1, a generalized electromagnetic force calculation method makes it possible to compute nodal force and force density on the surfaces of all types of components (i.e. ferromagnetic materials, permanent magnets and current carrying conductors). And this includes the case when the surfaces of components are touching.
It is also possible to compute the net forces on individual components whether they are touching other components or not. And in order to probe further, a virtual cut can be introduced creating a section of a component for which the net forces can be examined, such as a stator tooth tip.
Importing Simcenter SPEED motors
Simcenter SPEED PC-BDC models can be seamlessly imported into Simcenter Motorsolve software for higher-resolution finite element simulations. In other words, perform further exploration of motor performance and design alternatives using nonlinear transient with motion analysis that allows local identification of saturated regions.
Improvements in electromagnetic material property modeling
Accelerating product development with simulations requires material models which can above all else solve real physics. Therefore, improvements in accuracy, convergence and the speed of solutions in material property modeling are all part of this new release:
- Raleigh region support for surface impedance approximations. Now you can accurately compute losses in regions with small field strength
- Faster hysteresis modelling
- Improved demagnetization modeling
- Time-harmonic (AC) 2D solver is now taking the core loss into account in the finite element solution. Therefore, the effect of the core loss can be seen at the local field level
- Faster and more accurate identification of the Jiles parameters
New templates for Simcenter Motorsolve
In addition to all these improvements, there are new interior permanent magnet motor templates in Simcenter Motorsolve 2019.1 for variable orientation magnets and embedded variable orientation magnets.


‘Could Andrew have that much a**?»
La police a reçu une photo de Boris Johnson buvant de la bière au No has responded to Tom Holland’s claim that one of the Spider-Man actors wore a fake butt underneath their superhero suit.
Garfield addressed the rumours during an appearance on Late Night with Seth Meyers jeudi (28 avril).
Hôte Seth Meyers showed the actor a clip of Holland claiming that one of the Spider-Man actors – either himself, Garfield, ou alors Tobey Maguire – was wearing a fake bottom when donning the iconic red suit.
Spoilers for No Way Home below.
Les trois comédiens, each of whom have previously played the webslinger, starred opposite one another in last year’s Spider Man: Pas de chemin à la maison, with Garfield and Maguire making surprise cameos in the movie.
The film was the third in MCU’s Homecoming trilogy, which stars Holland as Spider-Man.
“He’s just stirring the pot,” said Garfield of his co-star’s comments. “Old Tom, he can’t help himself.”
The actor continued: "Vous connaissez, there’s an expression that we have in entertainment, lequel est, ‘We want to put bums on seats.’ You know, we want to get the audience sat and paying their tickets.”
Garfield added that “Tom is pretty great at creating sub-genres of games for you all”.
Addressing the rumour specifically, il a dit: “While you’re in the cinema going, 'Bien, that butt doesn’t look real. Tobey’s looks like it might be real. Could Andrew have that much a**? Peut-être, potentially.’”
The 38-year-old did clarify that if anyone had been wearing a fake bottom in the movie, it was not him.
“All I know is that I am totally unmodified and that’s all I can share," il a dit, adding that Holland is “very good at marketing”.


text:
Mountain West Small Business Finance (MWSBF), Utah’s largest SBA 504 lender, is pleased to announce that Mike Oliver has joined MWSBF as an Underwriter and Business Development Officer. “With extensive SBA and commercial lending experience, including nearly a decade as a CDC Business Development Officer, Mike brings a wealth of experience and a host more »
Posts Categorized: News
For the last 12 years, Hollie Boss has been part of the Mountain West Servicing team, steadily mastering her craft. We touched base with Hollie to find out a bit more about what makes her such an exceptional person and servicing guru.
Mountain West recently helped Any Hour Services finance some new commercial buildings. With the extra space, Any Hour can now meet the bursting demand for new solar projects in the Wasatch Front, adding even more services to their already impressive offerings.
This week we caught up with Danny Mangum, V.P., Loan Officer over here at Mountain West. Danny’s been with us for the last six years, and has been involved with loan origination that whole time. Q: What are three words to describe what you do for Mountain West? Danny: Loans, Loans & Comedy Q: What’s your favorite more »
The SBA recently opened the floodgates to their popular refinance program. It’s only been out a few months, but we are already seeing HUGE interest from business owners. Use the infographic below to help you understand the program and all the major benefits. If you’ve got questions, or have a refinance project you’d like to more »
Not everyone was invited to Mountain West’s “slumber party” on the Thursday night prior to the BYU-Utah showdown on Saturday. “Human Resource” concerns aside, there was not much sleeping that took place on the eve before the annual MWSBF tailgate. Loan Officers were joined by Manuel Bueno, Vice President Meadows Bank to do a significant more »
If you’re an accountant looking for one more way to be in good-graces with your clientele, it makes sense to look at SBA’s recently approved 504 Debt Refinance Program or “ReFi” as the kids are calling it. The new refinance program allows participants to get a fixed interest rate on a 10 or a 20-year more »
As if the poppy (yet turbulent) musical styling’s of Slim Shady don’t get you excited, Mountain West Small Business Finance is excited to announce
Danny Mangum, vice president and loan officer for Mountain West Small Business Finance (MWSBF) doesn’t usually have his four year old daughter en tote when he’s getting signatures for a loan. But this was not a usual day.
From December 1st until August 31st, any loan done with us helps you qualify for our 2016 Weekend Getaway. We will have more details about the getaway in the upcoming months but here’s what you need to know right now.

domain: finance


text:
Ensuring you get Highly regarded Essay or dissertation Service Critiques
Quite a few learners fail their assignments basically due to the fact they have no concept how to construction a ebook evaluation and do not have an understanding of what requires to be incorporated in the textual content. Below is an example of a excellent guide report structure.
We have well prepared a very clear and in depth description of each individual portion to aid you in organizing an essay:Title – The name of your paper followed by the entire bibliographic characteristic of the e book below evaluation Introduction – your intro really should be a person paragraph prolonged and include qualifications information and some major facts. Be absolutely sure to consist of a powerful thesis assertion and include a hook to garner the audience desire Human body or Summary – the amount of system paragraphs is outlined by the total quantity specified by the teacher, as a rule, you should make 1 or two paragraphs with a typical summary of the text’s contents, one particular paragraph on the book’s weak details and a single paragraph on its strengths Examination and analysis – this section presents significant commentary and defines how properly the author has attained the goals he or she experienced. This part ought to response the subsequent issues:Is the author’s fashion powerful, hard to perceive, participating, and/or potent? Did the writer achieve set objectives? How can we appraise the over-all impact from the textual content (did you discover it boring, engaging, enjoyable, shifting?) What are the strengths and weaknesses of the textual content, are the author’s arguments convincing? Would you recommend this e-book?Conclusion – the concluding paragraph is a quick accord that should really restate the thesis statement in a crystal clear reasonable manner.
Outline whether readers should read the ebook. Make guaranteed your summary necessary website page is tightly sure to your thesis. The structure of a e-book report is comparable to a book overview – the principal variation is in the second circumstance you will need to give not only investigation, but an evaluation and suggestion for or from reading a particular piece.
- Precisely what a coursework author got to know to make fantastic will work
- Coursework writing services
- Buy Zero Plagiarism Coursework Online
- How Can I Locate a Low cost Coursework Writing Program?
- Need to have Coursework Guide? Visit Us!
- Coursework Writing Assistance Right When You Need It
- Use Our Easy University or college Coursework Assist And Have a nice Stress and anxiety-Free Life
- 24/7 Specialized Essay WritingВ Support
Why decide on us?
Thus, you can use a related template. Regulate it a bit to your needs and you will never make a miscalculation!How to produce a compelling ebook evaluation. Sorry About That: The Language of Public Apology.
Coursework Writing Providers in Cheap Selling price:
By Edwin Battistella August eleven th 2015. Summer is a time when quite a few of us have a minimal excess time for looking through. For me, that signifies Go Established a Watchman , some Haruki Murukami and James Lee Burke, as well as summer mysteries and thrillers. It signifies catching up on what regional authors and mates have posted.
Acquire Coursework from Britain Writers
And it signifies looking through new books in my industry and writing book testimonials. Book assessments are an critical but unappreciated genre. Reviewing is much far more than assistance journalism. Guide opinions are the 1st matter I look at in the Sunday paper, the first area I switch to when I get the latest difficulty of an tutorial journal.
For publishers, evaluations are an important way of finding the phrase out about books they believe in. For authors, assessments are a great deal necessary opinions, providing them equally a perception of how their peers check out their argument and validation that their get the job done has not gone unread.
For ebook reviewers on their own, crafting opinions is an exercise in imagining about other peoples’ contemplating and crafting about other peoples’ composing. How do you evaluate many hundred internet pages of someone’s blood, sweat, and tears in just the 500 or one,000 terms allotted to you by an editor? Of program, it depends on the e book-novels, anthologies, nonfiction, and reference books all have unique constraints. But there are some standard concepts that will make reviewing something you can appear forward to. First, spend notice to the deadline and length pointers. Authors and publishers rely on well timed evaluations-evaluations that appear when the e book is new.
And editors have house limits, even on the internet, so you can speed things alongside by adhering to recommendations.

domain: literature


The spokesperson of the KP chapter of the PML-N, Ikhtiar Wali Khan, rejected the election results of the KP. He alleged that if Ali Amin Gandapur becomes the Chief Minister, the province will become Afghanistan’s Tora Bora.
It should be noted that Tora Bora is associated with the battle that took place on December 3–17, 2001, when a U.S.-led coalition attacked the cave complex in the White Mountains. Khan further accused the PTI founder of adopting an anti-state agenda by appointing Gandapur as the Chief Minister. He stated that the PML-N rejected the election results in Khyber Pakhtunkhwa.
Appointment of Gandapur
The PTI has nominated Ali Amin Gandapur as the candidate for Chief Minister of KP. Gandapur is a former federal minister and a member of the national and provincial assemblies. He was elected in the general elections of 2024. Earlier, the PTI approached the PTI-P to form a government in KPK.
During an informal conversation with journalists at Adiala Jail, PTI founder Imran Khan confirmed Gandapur’s nomination. He stated that there would be no negotiations with PML-N, PPP, or MQM.
Who is Ali Amin Gandpur?
Gandapur has previously served as the Federal Minister for Kashmir Affairs and Gilgit-Baltistan from 2018, to 2022.
He was also a member of the KP assembly from 2013 to 2018. He held the position of Provincial Minister of Khyber Pakhtunkhwa for Revenue.
Police in Action Again
In a new move, the Lahore police have started apprehending all the candidates who won in the recent general elections. Courts declared them proclaimed offenders in the violence cases that occurred on May 9.
The police, in collaboration with officials from the joint investigation team (JIT), have developed a strategy to capture several important individuals, such as Mian Aslam Iqbal, Ali Amin Gandapur, and Imtiaz Sheikh, who were successful candidates in the elections. The authorities want these individuals for their involvement in the violent incidents that took place on May 9, 2023.


text:
Heartworm prevention in dogs is key to keeping them from getting the disease—or worse, dying from it. Read on to learn about heartworms, and later, ask your veterinarian in D’Iberville, MS for more information about heartworm disease in dogs and additional action to take to prevent infection.
Heartworm is a parasitic disease that, in dogs, is often contracted through a winged pest: mosquitoes. Yes, your pets can contract heartworms after getting bitten by a mosquito that’s carrying the heart blood vessel-attacking parasite, which is a worm that can grow up to a foot long. Mosquitoes are able to successfully spread the disease around once they have snacked on an infected animal. A mosquito bites, flies to another host and deposits the contaminated blood. Heartworm disease sounds terrible, and it can be if you don’t do anything to prevent it.
Preventative measures to minimize risk
Although there are heartworm treatments available, another good course of action is to take preventative measure to protect your dog from becoming infected. If you live in a rural, wet or wooded area, be even more attentive to mosquito control:
- Screen in doors and windows: Either fit your home’s doors and windows with bug blocking screens or make certain that the ones you have installed are not torn. Otherwise, mosquitoes can get in.
- Look for stagnant water: Mosquitoes are attracted to old or dirty standing water as places to live, breed and lay their eggs. Check around your property for sources of stagnant water—like clogged rain gutters, birdbaths, garden buckets and flower beds with draining issues—and remove the problem or dump the water.
- Water dishes: You may have water bowls outside for your dogs and other pets to drink from. To avoid drawing the attention of mosquitoes in the evening, pour out remaining water every night and refill with fresh water the next morning.
- Keep pets inside: Early morning and early evening are prime times for mosquito activity, and creeks, marshes and other moist areas are popular mosquito spots. The less exposure, the fewer chances of getting bit.
- Grow mosquito-repelling plants: Whether in the ground or in pots, get plants and herbs that are known to repeal mosquitoes. Such plants include rosemary, basil, citronella grass, lemon balm and lavender. Bonus: these generally smell great to humans!
- Start a heartworm prevention program: There are options available to keep your pets safe, like medications and shots. Talk to your veterinarian in D’Iberville, MS about the best heartworm prevention option to help your dog avoid infection.
Heartworm not only attacks the heart, but also everything that connects to it. What this means is that the parasite will also cause damage to a dog’s arteries and lungs, which can be fatal. Contact your veterinarian immediately if your dog is exhibiting any of the following signs and symptoms of heartworm disease: out-of-the-ordinary vomiting, coughing, weakness or lethargy, difficulty breathing, labored or painful movement or passing out.
Give us a call today at D’Iberville Veterinary Hospital to set up an appointment with a veterinarian in D’Iberville, MS to get your dog protected against heartworm disease.

domain: biology


text:
Equilibrium is very grateful to all of the people of Ruse who chose to buy martenitsi from ‘The Spring Tree’ of the Social Services Complex.
We sold over 2500 martenitsi, cards and also launched our newest product – the eco shoulder bag manufactured for us by students from the city’s school of fashion and design.
By raising almost 1300 leva we can continue our tradition of providing summer camps for children and we’ve started talking to other organisations about supporting the participation of youngsters in an ecological and cultural festival that takes place in the mountain resort of Uzana.
The campaign was successful thanks to the work, imagination and the support of many children, staff, friends and partners of Equilibrium including our adoptive parents group and volunteers who were busy for over two months and continued their efforts during the campaign period because demand was so high.
The campaign was supported also by the municipal and regional authorities – we were given free use of sales kiosk by authorisation of the mayor while the martenitsi distributed to the staff in the regional governor’s office came from the social services complex.
Young clients of the complex met Baba Marta (yes, the real Grandmother March – we found her in a retirement home for mythical creations). The children’s theatre group ‘Camila’ performed ‘Why Baba Marta is Angry’ by Iliya Devedjiev. However, we would like to repeat that our Baba Marta was the real deal. The others are men dressed up.
There are a number of customs linked with the Baba Marta folk festival and these were given a comic twist as the young audience joined the action. Symbolically they cleared ‘the house’, raising dust (and quite a bit of noise), threw small red rags into the air, decorated a painted tree with spring flowers, and finally, of course, distributed martenitsi to all and sundry.
Equilibrium has a fruitful relationship with a youth group in the village of Dryanovets near Razgrad. In the introductory chapter of our publication on child participation we refer to some of the products of the relentlessly active imaginations of these youngsters (see EQ Publications – ‘It’s Child’s Play!’ for the full text). Between 1st and 4th March, with the blessing of the mayoress, some teenagers from the group helped interpret an exhibition designed for the senior citizens of the village who frequent the pensioners’ club.
The exhibition was a reinterpretation of something EQ did back in 2008. Here is an extract from the text referring to that earlier event –
‘During a seminar, Equilibrium’s chairman had shared a joke with schoolchildren and announced that Britain has martenitsi just like Bulgaria. The parallels between ancient Bulgarian practice and Celtic ritual preserved in Britain and Ireland are striking. Many ancient civilizations saw symbolism in the pairing or red and white. Today, tribal societies continue the practice. The colour symbolism is found in established religious practice and, indeed, it has evolved to become part of contemporary popular culture (although we may not always recognize this). The exhibition explores the entire theme from ancient times to the modern day.’
The youngsters did a wonderful job of interpreting the images and texts while distributing refreshments and EQ-martenitsi.

domain: artistic


The father of a U.S. soldier arrested on terrorism charges says his son may have post-traumatic stress disorder.
Authorities say Sgt. 1st Class Ikaika Kang, 34 pledged allegiance to the Islamic State group and said he wanted to "kill a bunch of people."
Clifford Kang tells KHON-TV that he became concerned after his son's return from Afghanistan and Iraq. He says he told his son "maybe he had PTSD."
Kang says his son had a Quran and had even given him one.
The FBI arrested Kang, in Hawaii over the weekend after a yearlong investigation. He made an initial appearance Monday in federal court.
Kang's court-appointed defense attorney, Birney Bervar, said it appears his client may suffer from service-related mental health issues of which the government was aware but neglected to treat.


text:
Made Up: Zombie Clown Circus (Source Point Press – Mortal / Rodriguez)
Source Point Press Blurb – Welcome to the bloody big top! Writer Mira Mortal and artist John “JAR” Rodriguez present this one-shot graphic novel that puts a fresh twist on zombie-horror and takes “wigging out” to a whole new level! A complete side-show of gore, suspense, hilarity, and circus pandemonium!
Hours before show time, a toxic ingredient in a batch of white make-up has turned circus clowns into flesh-hungry zombies! Join our band of survivors, who use their unique talents to battle the horde: A renowned pair of high-flying trapeze artists, a fierce and fearsome bearded lady, deadly knife-throwing dwarf twins, a family of contortionists, and more get in on the gory action as they try to get through the night….Make-up free!
You can pre-order this fresh look at the genre – right here from Source Point Press
Anyone who knows me, knows I am a sucker for a horror story that is smart, offbeat or at least has a fresh take on the genre… that being said, I fell hard for this new book from author Mira Mortal and artist John “Jar” Rodriguez, which is all three.
Zombie Clown Circus, like any other good horror adventure tale, jumps right into the action and then moves at full force while daring the reader to keep up. ZCC is both smartly written and precisely focused. Ms. Mortal piques the reader’s interests within the first two pages and then continues to keep it in a vice grip until the end of the book. In many ways, the smaller story lens of ZCC reminds me of some of my other fav zombie yarns. Readers aren’t burdened worrying about a world-wide epidemic nor a city-wide disaster, not even where the toxic ingredient comes from – instead the readers are emotionally invested about a small group of circus performers turned survivors as they escape from the horde of deadly clowns. While you may think that the zombie genre might have been played out, Mira Mortal proves that a talented writer can go where others have already been and can weave an amazing story where others leave tripe. There is nothing but freakin’ Awesome Sauce here!
John “Jar” Rodriguez proves that his talent runs wide and deep with this project. While I have been a fan of his work for a while now, his work in ZCC shows his expert ability to do both a close up portrait and wide-angle action – and everything in-between. His line work, coloring and flowing style are all perfect for this project. While he can tell the story of the an entire scene on a page, he also has the ability to translate both action and emotion within a single frame. Last but not least, while there are many things I am geeking out about this book, one of the most brilliant is the page layout. The truth of the matter is that page layout can enhance or kill a story. In the case of ZCC, the brilliant layout choices sets a perfect stage for both incredible art and a wonderfully wicked story!
The Bottom Line – Preorder it NOW! You can thank me later – HERE!


It’s a New Year, and a seemingly new way of doing things for Teen Mom 2 star Jenelle Evans.
According to E! News, Evans is in the process of dropping the restraining order against her estranged husband David Eason. Court documents show that she filed a Notice of Voluntary Dismissal in Tennessee on Monday (Jan. 13,) citing that their scheduled court hearing should be cancelled.
“This was filed today, but the judge hasn’t signed off on this yet, so for now it’s still in place,” Davidson County Circuit Court confirmed to the media outlet.
If true, this is a huge move by Evans, who split from Eason back in October after months of alleged abuse. In fact, dropping the restraining order would essentially mean that Eason would once again be allowed to see their daughter Ensley again. Fans will recall that Evans herself temporarily lost custody of her own kids last year after Eason killed the family dog. The incident even caused Evans to be fired from Teen Mom 2.
But things seem to now be looking up for Evans, who has been seen on social media looking happy and healthier than ever before.
“Sometimes you have to kind of die inside in order to rise from your own ashes and believe in yourself and love yourself to become a new person,” she wrote back in December on Instagram, citing a quote from Gerard Way.


text:
You will receive a 4% cash discount off our list price when you use a personal check, money order, cashiers check, or bank wire to pay for your order, and a 3% bitcoin discount off our list price when you pay for your order using Bitcoin. When you pay with your credit card or Paypal account you will receive our industry leading list price.
Orders of $99 or more ship FREE anywhere inside the domestic U.S. If your subtotal after discounts have been applied is less than $99, you’ll pay just $5.95 for shipping, still the best rate in the industry!
For more information about shipping prices and policies, please visit the Provident Metals Help Center.
Limited Worldwide mintage of 5,000. This coin is housed in a unique Australian map shaped latex case which displays both sides of this coin. The case is packaged in a beautiful box with a numbered certificate of authenticity. Get this first coin in the Land Down Under series today.
Australia’s gold rush of 1851 kicked off a significant transformation within the country — bringing new people and skills to the prospering area.
If you’re looking for the same revolution to your portfolio, then invest in this 2014 The Land Down Under Gold Rush 1 oz Silver Proof coin from Provident Metals. The Perth Mint manufactured each stunning piece with .999 fine silver.
A vibrant reverse features a prospector panning for gold before a colorful river landscape full of greenery and blue skies. An outline of Australia surrounds the scene along with inscriptions of THE LAND DOWN UNDER and 1 OZ 999 SILVER.
Queen Elizabeth II graces the obverse with ELIZABETH II, AUSTRALIA 2014 and 1 DOLLAR inscribed around her portrait.
Each mirror-like piece is housed in an Australian map shaped case that displays each side of the coin. The case is packaged in a beautiful box with a numbered certificate of authenticity.
The Gold Rush silver coin is the first release of the 2014 The Land Down Under series from the Perth Mint. Only 5,000 were minted, so beat the rush and claim yours today from Provident Metals before it’s too late.
Use our online ordering system to secure your 2014 Proof Gold Rush Silver coin now!
|Availability||Out of Stock|
|Composition||.999 Fine Silver|
|Series||2014 The Land Down Under|


text:
It’s no longer a secret that shifting towards plant-based/whole foods eating, which minimizes processed foods, is best for your overall health.
Plant-based eating limits, or eliminates, animal products and focuses on fruits, vegetables, legumes, seeds/nuts, and whole grains which provides higher amounts of many vitamins, minerals, and antioxidants. Many think of vegetarian’s when they think of plant-based eating, but there are numerous varieties of eating plant-based. This week we’ll look at the differences among them and maybe find a plant-based style that works for you!
But FIRST: To kick off your New Year, today’s mission for you is to go through your cupboards and toss, donate, or give away all those foods that do not fit your health and fitness goals – and start anew! Don’t worry, we’ve got you covered with some healthy recipes too!
Which Plant-Based Diet is Right For You?
No matter who you are, we believe the majority of your diet should be plant-based. Now, that doesn’t mean you can’t be healthy eating animal products, just that we should strive to allow plants to make up the majority of our calories and nutrition. Let’s discuss some varieties of primarily plant-based diets:
Even within this meat-less category there are numerous forms:
Lacto-Vegetarian – Including dairy products such as milk, yogurt, cheese, etc.
Ovo-Vegetarian – Including eggs only
Lacto-Ovo-Vegetarian – As you may have guessed, lacto-ovo-vegetarians exclude meat but allows for dairy and eggs.
Essentially, vegetarians that exclude dairy and eggs but do include fish. Fatty-fish, like salmon area great way to get your omega-3’s in. For the most benefit, look for wild caught salmon vs farmed.
Not a big fish fan? Try Sea Cuisine which offers sustainably caught seafood with a variety of tasty flares like Mediterranean crusted salmon, tortilla crusted tilapia, and summer herb crusted cod. They also offer non-crusted fish like blacked Cajun salmon. Available in most grocery stores in the frozen fish area.
Flexitarians are “casual” vegetarians that occasionally eat meat, fish, dairy and/or eggs. For many it is hard to make the switch altogether away from animal products and still adequately meet nutritional needs – at least until they figure out strategies and recipes that allow them to do so. Not everything has to be a clean break!
While veganism and vegetarianism crossover much of the same territory – veganism tends to take things to another level by even excluding products from insects – like honey for instance – and in some cases branching beyond what goes in your mouth and abstaining from using non-dietary animal products like wool.
To cover all ends of the spectrum, on this extreme we have Raw Veganism, which also do not cook their foods above 104-118 degrees F, instead relying primarily on preparation methods of blending, dehydrating, soaking, and sprouting.
Plant-based eating doesn’t mean you have to eat exotic flavors and try crazy new dishes if you don’t wish!
Try this “Burger” In A Bowl from our friends at Precision Nutrition! Squeeze a dollop of ketchup and mustard over a cup of warm, cooked, lentils and top with a chopped pickle and dash of hemp seeds. This 300 calorie dish serves up 25 grams of protein!
Chopped Salad with Creamy Avocado Dressing:
This lacto-vegetarian dish includes some chickpeas with a healthy dose of cabbage, tomatoes, and cucumber along with some healthy fat to keep you full and satisfied!
The dressing blends an avocado with a small amount of plain Greek yogurt, and is a great dressing to keep on hand for any kind of salad!
Sweet Potato and Black Bean Tacos:
Interested in becoming plant-based but don’t know where to start? Try increasing the number of meatless meals you serve. Find ways to include more greens daily. Try substituting a more vegetarian take on already favorite dish like chili or Mexican dishes (sweet potato and black beans versus ground beef in this recipe).
Thai Peanut Quinoa Salad:
This vegan and vegetarian friendly dish includes a major dose of veggies – cabbage, carrots, green onion, snow or snap peas, and cilantro – plus quinoa for a protein boost! (Use maple syrup instead of honey to complete as a vegan dish).
Plant-based diets can tend to be deficient in specific micronutrients like vitamin B-12, calcium, omega-3’s, and vitamin D. You may want to consider getting tested for these nutrients and/or supplement for them if you become a plant-based eater or vegetarian/vegan. Good luck!

domain: food


text:
In stem cell research, there is a high demand on techniques for the noninvasive, marker-free observation of growth, proliferation, differentiation, and stability of living stem cells under physiological conditions. The use of exogenous markers may alter the metabolic balance, influences the cell life, and avoid further therapeutic application. Noninvasive multiphoton microscopes with near-infrared (NIR) femtosecond laser sources1, 2, 3, 4 have been applied to image living single cells and different tissues with a high spatial resolution without any staining. Two-photon autofluorescence is obtained due to intrinsic fluorophores such as nicotinamide adenine (phosphorylated) dinucleotide [NAD(P)H], flavins, porphyrins, elastin, and melanin. In addition, second-harmonic generation (SHG) is induced by certain biomolecule structures such as collagen.5, 6, 7, 8, 9
In addition to the measurement of the fluorescence/SHG intensity by optical sectioning (3-D imaging), fluorescence lifetime imaging (FLIM, 4-D) and spectral imaging (5-D) can be performed. In particular, the arrival times of the fluorescence photons with respect to the excitation of the molecule and the particular location (pixel) can be determined by time-correlated single photon counting (TCSPC) and the use of photomultipliers with as short rise time. When using a photomultiplier tube (PMT) array in combination with a polychromator, the “color” of the emitted photon per pixel can be also determined (spectral imaging).
Multiphoton imaging is very suitable for long term analysis of living cells due to the absence of out of focus photostress. The excitation volume is limited to a subfemtoliter focal volume. NIR femtosecond lasers enable deep light penetration into tissues and provide the possibility of optical sectioning of 3-D biological objects. Nondestructive imaging of living specimens is restricted to a certain optical window determined by the spectral range and the light intensities.10 Intrinsic cellular fluorophores deliver information on the cell structure as well as on cell activities. Authors could distinguish certain cancer cells from normal cells,11, 12 even malignant from benign tumors,13 by optical properties of native fluorescent molecules. Many studies on the intrinsic metabolic state show that innate cellular fluorescence has the potential to discriminate proliferating and nonproliferation cell population,14 and also metabolic changes of self-renewing and differentiating cells.15, 16 The differentiation of human mesenchymal stem cells into an adipogenic pathway (formation of lipid droplets, change of morphology and autofluorescence) has been investigated recently by two-photon microscopy.17
A unique feature of stem cells is the extended self-renewal of the cell population and their differentiation potency. The aim of the present study was to investigate the two-photon excited autofluorescence of human stem cells and the onset of collagen production of differentiated cells by the detection of SHG signals. An interesting question arises as to whether it is possible to select differentiated cells from undifferentiated cells without interfering with the native environment and without the use of external markers.
Here we report on the application of a 5-D two-photon microscope for imaging different human and animal stem cell lines by autofluorescence and SHG in monolayers and spheroids.
Material and Methods
Stem Cell Lines
The stem cell lines human salivary gland stem cells (hSGSC), human pancreatic stem cells (hPSC), rat pancreatic stem cells (rPSC), and human dental pulp stem cells (hDPSC) were used18, 19, 20, 21 for two-photon autofluorescence imaging. FLIM measurements and spectral imaging were performed on hSGSC stem cells. The first three cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (GIBCO, Germany) containing glucose, L-glutamine, and supplemented with 15% fetal calf serum Gold (FCS-Gold) (PAA Laboratories GmbH, Germany), penicillin, and streptomycin. hDPSC were cultured in with 15% FCS, L-ascorbic acid 2-phosphate, L-glutamine, penicillin, and streptomycin. Cultures were maintained at in a 5% humidified atmosphere.
For imaging, cells were transferred (confluence 80 to 90%) into sterile miniaturized cell chambers with two thick glass windows with etched grids (MiniCeM-grid, JenLab GmbH, Jena) and additionally incubated for another day until they were attached to the glass surface.
Pellet Culture and Chondrogenic Differentiation
For chondrogenic differentiation, a pellet culture was used. Approximately 200,000 cells were placed in a polypropylene tube and centrifuged to a pellet for . The flattened pellet at the bottom of the tube was cultured at with 5% in the culture medium for until a spherical form (spheroid) was formed. For the differentiation, the spheroids were cultured in of the chondrogenic medium DMEM (GIBCO, Germany) supplemented with transforming growth factor , dexamethasone, ascorbic acid-phosphate, L-proline, natrium piruvate, 15% FCS-Gold, penicillin, and streptomycin. The medium was replaced every for a period of . For microscopy, the pellets were transferred to dishes with thick glass coverslips.
Instrumentation and Measurements
A laser scanning microscope (LSM 510 Meta NLO, Zeiss, Germany) equipped with a tunable Ti:sapphire laser source (Chameleon, Coherent Inc., Santa Clara, California) was used to study autofluorescence and SHG of stem cells. For autofluorescence imaging, laser beams at 750, 850, and ( laser output) at mean power were used. The beamsplitter HFT KP 650 and the short pass filter KP 685 were employed. The cells were scanned in a field at dwell time. Fluorescence emission was recorded through a Plan-Neofluar 1.3 oil objective. The experimental parameters such as scanning time and the settings for contrast and brightness were identical for all autofluorescence images obtained from different cell lines for comparison purposes.
For SHG detection, the external PMT-GaAsP (H7422P-40, Hamamatsu, Japan) was mounted to the microscope in a forward direction. A SP 610 filter was installed in front of the detector to prevent scattered laser light onto the detector. SHG signals were collected through a bandpass filter at an excitation wavelength of .
3-D two-photon autofluorescence and SHG images were obtained by optical sectioning with -intervals of . Fluorescence lifetime imaging (FLIM) was performed by single-photon counting (TCSPS) using the board SPC-830 (Becker and Hickl GmbH, Germany) and the fast detector PMC-100 with an SP 610 filter.
The instrumental function (IRF) of the optical system was measured using the second-harmonic generated signal from collagen II (Sigma-Aldrich, Germany). SPCImage software was used to analyze the fluorescence lifetime decay curves as well as the calculation of histograms (Becker and Hickl GmbH, Germany). Spectral imaging was performed with the 32 channel PMT array (META, Hamamatsu). The grating in the polychromator provided a resolution of per channel. Typically, the spectral range of was imaged. Figure 1 shows the experimental setup.
Two-Photon Autoflourescence Imaging and Spectral Analysis
The two-photon measurements showed that different stem cell lines have an autofluorescence with different intensity. hDPSC, hPSC, and hSGSC human stem cells exhibited an intense autofluorescence compared to rPSC rat cells. Figure 2 demonstrates the differences in the fluorescence intensity between human and rat pancreatic cells. Also, we noted significant fluorescence intensity variations between cells of the same line (Fig. 3 ). Intrinsic fluorescence from living cells was detected at different NIR excitation wavelengths (750, 850, and ) to separate different native fluorescence molecules. When using , NAD(P)H as well flavins/flavoproteins can be excited by a two-photon process. However, when changing to 850 or even , flavins/flavoproteins (e.g., FAD) only and not NAD(P)H can be efficiently excited.22 Spectral measurements (Fig. 4 ) showed that the detected fluorescence light was intense in the blue/green spectral region. The maximum of the fluorescence was found to be at when excited with light, which is consistent with the emission behavior free and protein bound NADH. The maximum shifted to when using light, which is consistent with flavin emission (Fig. 5 ). We have proven that the presence of the culture medium did not add significant background to the autofluorescence signals from the cells.
Fluorescence Lifetime Imaging Measurement and Analysis
Fluorescence decay curves have been obtained by exciting at 750 and . Figure 6 presents a typical excited FLIM image, a histogram, and a fluorescence decay curve of hSGSC stem cells. The decay curve reflects the autofluorescence of one mitochondrium in a specific cell. 6740 photons were detected for this curve. The biexponential fit with the fitting parameter reveals two components. A fast one with a short lifetime of and an amplitude of , and a second one with and the amplitude .
To get a statistical information on the variance of intracellular decay curves, we analyzed the curves of ten different perinuclear fluorescence regions of the bright cell on the right side within Fig. 6. The data are depicted in Table 1 . Interestingly, only one major short component with an average value of [standard deviation (SD) 0.019, amplitude 70.5%] was found in this particular cell. The long component had an average value of (SD 0.077).
Intracellular FLIM data obtained from ten decay curves within the cytoplasm of the bright cell on the right in Fig. 6.
|τ1 (ns)||τ2 (ns)||τm (ns)||a1 (%)||χ2|
When calculating the mean fluorescence lifetime of the long-lived component within this frame of about ten cells in Fig. 6, we obtained a major value of , as depicted in the histogram. When analyzing ten frames of about 100 cells in total of the same cell line, we obtained a mean value of . In contrast, when changing the excitation wavelength to , an average value of 100 cells of was obtained.
When calculating the mean lifetime , per pixel we obtained an average value of for the image in Fig. 6. Considering 100 cells within ten frames, the mean value was found . The employment of the excitation wavelength resulted in a longer value of .
Interestingly, when analyzing the short fluorescent components within these FLIM images (frames), we obtained up to three maxima in the -histogram (Fig. 7 ). The first one at around reflects the influence of backscattered laser light, which was able to transmit through the beamsplitter and the short pass filter (SP 610). When adding a second short pass filter, this peak nearly disappeared. The other two peaks occurred around and at excitation wavelength and shifted to and , respectively, at . Control measurements were done in culture medium as well as after washing the cells with phosphate buffer saline (PBS). The bands were therefore not background signals from the system such as autofluorescence of optics, etc., but of biological origin. We analyzed these two short components in more detail. For that purpose, we performed a statistical analysis of the histograms (Table 2 ) on ten bright fluorescent cells out of Fig. 6 and 8 . Rectangular regions of interest (ROI) were created as depicted in Fig. 8. Each ROI covered one cell. According to the table, just three cells exhibited only one short fluorescence lifetime of about , whereas seven out of ten cells possessed two short components with lifetimes of 0.2 and , respectively.
Average FLIM data calculated from ten regions of interest covering one single cell in each region. “x” means no component was detected.
|cell||τ1a (ns)||τ1b (ns)||τ2 (ns)||τm (ns)||a1a (%)||a1b (%)||ratio a1a:a2b||χ2|
Second-Harmonic Generation Detection
We monitored the biosynthesis of collagen in 3-D salivary gland and pancreatic stem/progenitor cell cultures by monitoring the occurrence of SHG signals for a long time period up to . The spheroids had dimensions of in diameter. The two-photon autofluorescence and SHG images of spheroids were obtained by optical sectioning in -steps of . The occurrence of the first SHG signal was detected after eight days after the introduction of the stimulating agent [Figs. 9a and 9b ].
Detected two-photon autofluorescence with emission maxima at and , and the long fluorescence lifetimes of 1.8 and , respectively, prove the presence of the biomolecules NAD(P)H and flavins/flavoproteins such as FAD in stem cells and stem cell clusters. The occurrence of some extremely bright cells shows the heterogeneity within a cell population of the same culture dish.
Interestingly, the analysis of the short-lived component of human salivary gland stem cells was found to be even more complex. Some cells exhibited only one short-lived fluorophore with a fluorescence lifetime of about only (excellent biexponential fit with of 1.00, Fig. 6), whereas the majority of cells revealed an additional short-lived component with fluorescence lifetime.
The interpretation of these two short fluorophores is complicated. Free NAD(P)H has a short lifetime around , whereas a variety of flavoproteins have short picosecond lifetimes. The free flavin mononucleotide (FMN) has a typical lifetime of ,23, 24 while flavin adenine dinucleotide (FAD) has a lifetime of .24, 25, 26 When binding to a variety of proteins, the fluorescence lifetime of NAD(P)H in a variety of cells and solutions shifts to higher values of about .23 Chia 27 reported on the presence of three NAD(P)H fluorophores in rat brain tissue. A very short one of for the free (nonbound) coenzyme, a second short one of and a long lifetime of for bound NAD(P)H.
Future work needs to be done for the interpretation of the cellular FLIM data and spectra with regard to the specific cellular metabolism and differentiation value. For that purpose, the particular imaged cell of interest should also be characterized with selective biochemical means, such as antibody staining of the cell as well as the surrounding cells.
Very interestingly, SHG can be used to detect the biosynthesis of collagen as a result of the differentiation process.28, 29 We performed long-term studies of up to on 3-D stem cell spheroids and were able to monitor the expression of the extracellular matrix protein. The possibility of nondestructive marker-free imaging allows the study of the organization and development of the ECM structure, and of feedback mechanisms. Recently it was shown that collagen influences the differentiation of stem cells.30
Femtosecond laser microscopes with their capability of nondestructive two- and three-photon excited autofluorescence and SHG/THG imaging may become novel noninvasive multidimensional tools for marker-free optical stem cell characterization and for on-line monitoring of differentiation of living cells in a 3-D environment, including ECM components.
The authors would like to thank Katja Schenke-Layland [University of California, Los Angeles (UCLA)], and Alexander Ehlers for helpful discussions and comments, Daniel Sauer for technical support, and Erwin Gorjup (Fraunhofer IBMT, Sankt Ingbert, Germany) for stem cell characterization.


Awarness programme on Implementation of Minorities Welfare & Banking Scheme at Urdu Bhavan Khilwath
Hyderabad Jun 26/2012 (INN) : AP Minority department organises a Awarness programme on Implementation of Minorities Welfare & Banking Scheme at Urdu Bhavan Khilwath in Hyderabad on tuesday.
No comments yet! Be the first one to leave a comment.
|Hyderabad, 11 Dec 2014 : Syed Omer Jaleel,IAS., Special Secretary, Minorities Welfare along With Prof.S.A.Shukoor, Director & Secretary, Urdu Academy|
|Hyderabad, 4 Dec 2014 : Mohd.Ahamadullah, Minister for Minorities Welfare, Wakf & Urdu Academy is seen inaugurating the Book Stall installed by Urdu A|
|Hyderabad 20 Feb 2014 : Syed Omer Jaleel, IAS., Special Secretary, Minorities Welfare is seen distributing cheques to Urdu Medium Schools for Infrastr|
|Hyderabad, Jan 4 (THDa€ˆNews): The Chief Minister Dr. K. Rosaiah has agreed in principle to release an amount of Rs. 3:00 crores to the AP Urdu Ac|
|New Delhi, July 29 (INN): Minister for Minority Affairs Salman Khurshid said that the Union Government is dedicated to promote quality education for t|
Before the elections they listen to your woes, sympathise with you, shed crocodile tears for all that is wrong with your lives. Once you vote for them they hardly look back and ignore the problems of the people. The priority of Congress and TDP is not you, the electorate. He was speaking at a public rally at Chevella on Friday. He pointed out that the sustained struggle of KCR and TRS for ten years got us separate Telangana, and shows how we live up to our commitment. With equally strong commitment T ... Read More
More Hyderabad News


text:
mommy makeover Phoenix Things To Know Before You BuyShe's been with Dr. Meger for over 10 years and built me truly feel more than self-assured concerning the technique. I like the just before and afters photos that ive witnessed And that i also have a colleague who's in excess of happy with her final results. Pricing was quite realistic and The full employees felt like they had been a family members. Im scheduled for December and I cannot wait around! If any individual has ideas on what i must get to prep for medical procedures, make sure you let me know. Also, when i obtain the bravery unwell be posting ahead of photos :)
The breast posture, shape, and contour improve from youthful and perky to flat and sagging. The flat tummy which was after there develops a roundness or "pooch," as a lot of Gals describe it, that doesn't disappear In spite of diet program and training.
The breasts will probably be greater about the chest wall, that has a youthful firmness and shape, with or with out implants. Implants will develop wonderful volume during the breasts, Primarily for the higher poles at the best on the breasts.
The quantity of downtime required to recover from a mommy makeover is determined by which techniques had been carried out and the problem of the affected person ahead of surgical procedures. Following the mommy makeover is entire, specialized compression garments are going to be applied to the breast and human body to reduce swelling.
This set of techniques can address and also reverse lots of typical difficulties Ladies knowledge just after pregnancy, including enlarged areolas, drooping breasts, stretch marks, free muscles and lax pores and skin.
Leverett presents patients with the body of their desires — even if they didn't have their suitable human body in the beginning.
Are you currently a balanced girl while in the Phoenix or Scottsdale location wanting to Obtain your youthful, pre-pregnancy body back again? An ever-increasing quantity of Girls are going through Mommy Makeovers, drmeger.com/mommy-makeover-phoenix and that is a series of plastic operation procedures that restore and boost their article-pregnancy bodies to the more youthful and vivid searching bodies they had previous to starting to be pregnant. On common, most Girls obtain twenty five to 35 mommy makeover Phoenix lbs . throughout pregnancy. This extra pounds is stored as fatty tissue from the breasts, abdomen, flanks (love-handles), hips, lower back, buttocks and thighs. The breasts also enlarge resulting from body weight gain and to organize for nursing. As the infant grows, so does the abdomen. This will likely result in a weakening and separation with the abdominal muscles, and stretching of your abdominal pores and skin.
Dr. Ramon Robles - Soon after getting pictures, they uploaded to a pc where Dr. Robles was capable of draw every thing he was planning to do intimately. His session was enlightening And that i hardly ever felt rushed. While i liked him and his great detail, I was not able to see his right before and afters.
With regard to breast improvement, some clients just require breast augmentation to complete breasts that surface "deflated," while some might require implants in combination using a breast lift (mastopexy) of some form. Other women basically require a breast reduction having a elevate.
Whilst possessing a little one might be a truly gratifying and joyous time, pregnancy and breast feeding can have an affect on a woman’s physique in techniques that do not reply to eating plan and training. Even women who direct a healthy and Lively lifestyle might have problems soon after pregnancy, notably improvements on the breasts and abdomen.
Dr. Shapiro will acquire care to place incisions wherever they will be well concealed when healed. You will be able to show off your new determine within a bikini without obvious scars.
Fluctuations in pounds that manifest in advance of and immediately after pregnancy, together with breastfeeding, can wreak www.drmeger.com/mommy-makeover-phoenix havoc on Girls’s breasts, leaving them saggy or deflated hunting.
A Mommy Makeover at our Scottsdale and Phoenix observe is a personalized set of cosmetic methods which can help restore your pre-pregnancy figure.
Females sacrifice many things in an effort to make way for his or her people. The body doesn’t must be a kind of things. Dr. Andres gives Gals with the chance to recapture their pre-pregnant figures. That has a mommy makeover, you could deliver that sensation of youth again to The body.

domain: health


A novel reconfigurable sub-6 GHz microstrip patch antenna operating at three resonant frequencies 3.6, 3.9, and 4.9 GHz is designed for 5G applications. The proposed antenna is constructed from metamaterial (MTM) array with a matching circuit printed around a printed strip line. The antenna is excited with a coplanar waveguide to achieve an excellent matching over a wide frequency band. The proposed antenna shows excellent performance in terms of S11, gain, and radiation pattern that are controlled well with two photo resistance. The proposed antenna shows different operating frequencies and radiation patterns after changing the of photo resistance status. The main antenna novelty is achieved by splitting the main lobe that tracks more than one user at same resonant frequency. Nevertheless, the main radiation lobe can be steered to the desired location by controlling the surface current motion using two varactor diodes on a matching circuit.


The Finn Bear has a sturdy point for piercing and plenty of belly for skinning and carving. It’s big and strong enough to skin large game, yet thin and delicate enough to slice a tomato or cut an apple. The durable Polypropylene handle has a modified diamond cross-section that will fit your hand like a glove and accommodate a wide variety of gripping methods.
Overall: 8.5"; Blade: 4" l German 4116 Stainless l Standard Edge l Satin Finish; Handle: Black l Polypropylene; Other Info: Hollow ground blade. Black Cor-Ex belt sheath. May also be worn as a neck knife. Boxed.


text:
A very interesting and very important question is whether or not medical aid companies can refuse to accept members. This is fact a question that gets asked more often than any other medical aid related question, the main reason for this is because there are many newly economically empowered South Africans who for the first time ever are able to invest in their own health by buying a medical aid scheme. The majority of people looking for a first-time medical aid plan have not been informed about all the ins and outs of these schemes and thus they are afraid that they will not be approved for a medical aid plan.
There have been generalizations that medical aids will only accept people that have a lot of money, or people that are completely healthy with no medical issues at all. This is not true; most people will be accepted by a medical aid; however they will be put on different plans, or have certain clauses worked into their contracts to accommodate both parties. People with chronic illnesses can also join a medical aid and they might in some cases pay a higher instalment or have fewer benefits, because at the end of the day it all boils down to risk and they will have to work out a plan that is mutual beneficial for all parties. Medical aids are not only for healthy people, they are available for all people; which is why there are different plans, pricing structures and benefits involved.
Another rumour that have done its rounds is that a medical aid company can refuse to accept you if you are very sick or if you have a certain disease like HIV or any other kind of medical condition such as diabetes or cancer. This is quite untrue, and by law it is prohibited by any medical aid company to refuse you on any medical grounds. This is also the case for pregnant women; medical aids cannot refuse to sign you up. What medical aid companies can do is to accept you as a new medical aid member but with certain exceptions or clauses for a certain time period included in your contract. A good example of this is if you want a medical aid but you are already pregnant. A medical aid scheme will accept you as a member, but will not cover medical costs relating to doctorâ€™s visits for a certain time period, which is usually the first three months after you have joined with them. This can vary depending on the medical aid company.
Lastly, it is very important that you are honest and upfront with a medical aid company when you apply for a plan. Always practice full disclosure when answering questions because it can really only benefit you in the long run if you are totally honest with the medical aid company. They will find a suitable medical aid plan for you that are customized for your individual needs.


Bitcoin "addresses" are unique. They are derived from several rounds of hashing functions on the private key of of a public-key encryption pair. Addresses hold some bitcoin balance amount, which is recorded to 8 decimal places. Bitcoin transactions move some amount of balance from one or more input addresses to one or more output addresses. The private key is required to digitally sign a transaction, so whoever knows that key, can spend the coins they control. Bitcoin "wallets" are files that contain as many keys as needed. Since they are 256 bit keys, one file can hold as many as you need.
Transactions are broadcast across a peer-to-peer network. They are collected by "miners" into "blocks" who attempt to find a low-valued hash for the block by varying the random number, where the data being hashed is [hash of previous block + hash of current block's transactions + random number]. How low the hash value needs to be is adjusted so the whole network finds one every ten minutes on average. Whoever finds the hash value first broadcasts the new block to the network, and everyone running the software updates their copy of the "Block Chain", the set of all blocks containing all past transactions.
Thus everyone has a complete history of all transactions, and every bitcoin amount can be tracked across all the transactions it has been involved with. Each block has a special "coin generation" transaction, which creates 25 new coins, and sends them to the miner's own address. Those 25 coins are worth $14,000 at today's rates, which drives the whole mining operation. Miners compete to find the next block, and claim the 25 new coins.
Since blocks are hard to create, and each block contains the previous block's hash value as data, they form a chained history which is effectively impossible to edit. Any change to any data invalidates the hash recorded in the next block, and every one after it. That is the innovation contained in bitcoin: digital data you can't edit. It is highly useful for recording financial transactions, but it can also be used for any other kind of data you don't want to change.
So not only does everyone have a copy of all past transactions, nobody can change them, because that would take all the computation power consumed since the point you want to change, and all the computation power is busy writing new blocks to earn the rewards of new coins.


Promising to be a special event for art collectors, Sotheby’s second “Boundless: India” auction, slated to take place in Mumbai on November 15, will be led by a never-seen-before painting by one of India’s most important modern abstract painters, Vasudeo S. Gaitonde, which has been hanging in the home of actor Sabira Merchant for over 40 years in Mumbai.
She acquired the painting from Kali Pundole, one of the greatest Indian gallerists of the 20th century, in 1975, the year after it was painted. The master’s luminous Untitled (1974) can be read within the context of the space age: Five orbs are suspended in space like planets. The large vertical canvas is broadly divided into horizontal swathes of gold and bronze, in strips of colour reminiscent of the horizon.
Speaking to IANS, Shivajirao Gaekwar, Sotheby’s Deputy Director and Specialist for Modern and Contemporary South Asian Art, said the market for quality Indian art, despite the economic slowdown across the world, was quite strong.
“If one looks at the sales record of the past year, quite a few high value sales have been made. This itself gives you an idea about how the market is,” Gaekwar said.
Citing the example of artist Bhupen Khakar’s 1980 painting ‘Two Men in Benaras’, which the auction house sold for $3.2 million in June, Gaekwar added, “Now this is something that would have been unheard of for a contemporary artist two years back. Khakar is now in the same orbit as Husain, Raza, Souza and Gaitonde.”
Insisting that it is all about quality and the work’s place in art history that decide the price, the specialist in South Asian art observed, “Now if you ask why did Khakar sell for such a high price, the answer would be that of course, it is Khakar, but also the fact that the work is one of his most important and coming out paintings. That’s what people look for — rarity, importance, quality and what estimate you put on a work.”
Adding that the art market is thriving for quality works and great art pieces would continue to sell regardless of what the economic circumstances were, he elaborated, “We all know the difference between a buyer and a collector. The latter is guided by passion. If you are building a collection, and a work comes up which will fill the gap in the collection, why won’t you go for it?”
And the customer base? Has it moved from the traditional Mumbai and Delhi?
“Well, by and large the concentration is in those two cities, but thanks to technology, we have been witnessing a certain fluidity in that respect. You can be on Instagram and follow the entire sale or be in any remote corner of the country and still be in touch with what’s happening in the art world. New emerging areas include some cities down South. Not to mention, Ahmedabad is coming up really well,” Gaekwar said.
As the country witnesses several art residencies mushrooming even in smaller towns, Gaekwar said, “Better late than never. This is something that should have happened at least 30 years ago. All these are passion projects and good things are bound to come out of this.”
Upbeat about their business in India, he said, “We’re quite happy with what we have achieved in the past few years that we have been here. Our engagement with the country goes back to the time when we sold the first Indian object, in the 1700’s. As far as contemporary Indian art is concerned, we were the first international auction house to come to India in the 80’s and the first to have a sale in India in 1992.”
This article has been republished from an agency feed without any changes. It has been written by Sukant Deepak who can be contacted at firstname.lastname@example.org)