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a couple days ago i woke up and my cat (11 years old) was having trouble walking, and it seemed like her rear left leg wasnt working properly. I was really worried that she might have broken it, but she didnt seem to be in pain, even when i touch her leg, she just seemed scared. Usually she runs away from people when they go near her, and she never lets anyone pick her up, but she was so weak that all she did was mew a couple times and then stop. I needed to get to school, so i told my dad to take her to the vet and i left her wrapped up in a blanket. while i was at school, my dad called me and told me he couldnt find her, but then later called me and told me that he found her hiding under a table and she wasnt eating or drinking anything. When school was over, my best friend (as usual) drove me home and he came inside and we checked on the cat, who was now hidng under my bed (she sumhow made it upstairs. My friends looked at her and said that she looked really bad, and that we needed to get her to a vet right away, so i wrapped her in a sweatshirt and we got in his car and sped to a vet. We went in and told them we had an emergency, and they took us and gave her a check up. They said that they couldnt find anything wrong with her, but her blood pressure was a little low and she was a little bit dehydrated (which made sense, because she wasnt drinking all day). They said their best guess was that it was a stroke, and they recommended letter her stay overnight. My dad said she could stay overnight, but he didnt want to pay for any blood tests or anything (he's not a very big animal lover at all). He picked her up the next day while i was at school after spending the night, and my friend and i called the vet on our way to my house to ask her how she was. The vet said they didnt have any results, because my dad didnt want them to do anything, which pissed me off. They told me to give her pedialyte to keep her hydrated, but she wasnt eating or drinking anyway. When we got home, i found my cat laying on the floor, weak as before. We picked her up and tried to give her pedialyte using a syringe. She was slow to take it, and it took me a while to get her to drink it, and she only took a few drops. I also tried giving her some food, which she just nibbles at once and dint eat. I felt like she was going to die that night, and i slept near her. Last night i woke up a few times and found her in different spots, first on the other side of the couch she was sleeping on, then down the basement, then opn the floor near me (each time i carried her back to the couch.) I thought this was a good sign, because she was moving around and walking a little bit. Today after school i went to my cat and gave her sum pedialyte, which she seemed to drink down a lot easier, only missing a few drops, so i thought she was doing better and would probably be ok. she also started mewing at me, and i guessed that she wanted food, so i gave her sum and she ate some of it (about a little less than half a can). I thought she was doing a lot better and would be fine. Some other things i noticed was that she smelled kind of strange, which i thought smelled kind of like the grape pedialyte. One thing that scared me today was that i was lying down and watching her, and her eyes moved in opposite directions outward, until i could only see the whites of her eyes. I jumped up, scared, and she quickly returned to normal. A couple times i saw her eyes seem like they were drifting, but not quite. She's been trying to walk around and run away from me, and is started to get mad again when i pick her up. I held her while she walked around for a little bit, and the whole time she seemed like she was trying to get away from me, which i thought meant she was returning to her normal self. The only things that are strange right now to me, and dont seems like shes improving, are that she has a bit of a funny smell and her eyes are slightly drifting to the sides once in a while (i think, but not sure if shes still doing it. anyway, i read sum stuff on the internet and from all of the information i got, it seemed like she was not improving and that she may be dying at any time. Im really scared. Can someone please give me some information/advice, or tell me wat is going on?
post #1 of 101
11/10/06 at 5:29pm

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‘We Got Married – Global Edition’ Gets an Air Date
On April 2, MBC every1 announced that the first episode of We Got Married – Global Edition will broadcast on television on April 7.
The show will air on MBC Every1 on April 7 at 11:30 PM, MBC Drama on April 7 at 1AM, MBC MUSIC on April 9 at 9PM, and MBC QueeN on April 10 at 11PM (KST).
The Global Edition has been gaining much attention and interest with 2PM’s Taecyeon virtually marrying Taiwan’s Emma Wu (Gui Gui), and FT Island’s Lee Hong Gi marrying Japan’s Fujii Mina.
The show will be aired in 21 different countries, including Taiwan, Australia, Hong Kong, Singapore, and more through an agreement with Singapore’s Sony Pictures Networks Asia.
The cast held a press conference on March 28, which as aired live through Youtube with English interpreters.
Photo Credit: MBC
On the June 30 episode of Mnet“s “SIXTEEN,” J.Y. Park announced the elimination of the show”s fourth contestant.
The contestant to be eliminated was selected from Minor A team which earned 109 votes, whereas Major B team earned a total of 209 votes. Minor A Team members Chaeryeong and Jiwon couldn”t hide their anxiety at the prospect of possibly being eliminated. Chaeryeong said, “I get nervous every time I go on stage so I think I”ll be eliminated.” Jiwon also revealed her feelings that she might be the one to be eliminated, claiming, “I feel anxious because I feel like I will drop out this round. I don”t feel like I have a chance anymore.” . ... Read more
Exactly one year after releasing his first mini album with K-Pop stars like VIXX”s Ravi and Aron of NU”EST, creative performer Chad Future has released his 2nd Mini Album today on iTunes! . ... Read more
Actress Lee Si-yeong has spoken about the tabloid rumor insisting there is a private video of hers going around. She has siad the rumor is groundless and will take stern measures against such rumors. . ... Read more
TEEN TOP is doing well with new album, “Natural Born TEEN TOP,” managing to stand out in the fierce girl group battle of the summer by taking home their first trophy on a music show via “The Show” on June 30. . ... Read more
BtoB’s “It’s Okay” Will Brighten Up Your Day Written by Morgan On June 30, 2015
BtoB have made their comeback with the soulful ballad, “It’s Okay”, on June 29. However, ‘it’s okay’ is not how I would describe this song and MV at all. By finding complexity in simplicity BtoB have created something that is more than just okay. . ... Read more

domain: entertainment


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Food recall warning: certain cookie dough, pie shells and tart shells due to E. coli warning
The Canadian Food Inspection Agency has added certain cookie dough, pie shells, and tart shells to their recall list due to E. coli contamination.
On Friday, CFIA warned of possible E. coli contamination in certain products sold nationally.
The brand names and products are sold nationally and consumers are warned by CFIA not to eat them.
Some of the products on the recall list are as follows: In-Dey-Go Fundraising Inc, Apple Valley, and Mildmay Cheese Haus.
For a full list and more information the CFIA website has more.
© 2017 Global News, a division of Corus Entertainment Inc.


text:
Alkyl hydroperoxide reductase enhances the growth of Leuconostoc mesenteroides lactic acid bacteria at low temperatures
AMB Express volume 5, Article number: 11 (2015)
Lactic acid bacteria (LAB) can cause deterioration of food quality even at low temperatures. In this study, we investigated the cold-adaptation mechanism of a novel food spoilage LAB, Leuconostoc mesenteroides NH04 (NH04). L. mesenteroides was isolated from several spoiled cooked meat products at a high frequency in our factories. NH04 grew rapidly at low temperatures within the shelf-life period and resulted in heavy financial losses. NH04 grew more rapidly than related strains such as Leuconostoc mesenteroides NBRC3832 (NBRC3832) at 10°C. Proteome analysis of NH04 demonstrated that this strain produces a homolog of alkyl hydroperoxide reductase––AhpC––the expression of which can be induced at low temperatures. The expression level of AhpC in NH04 was approximately 6-fold higher than that in NBRC3832, which was grown under the same conditions. Although AhpC is known to have an anti-oxidative role in various bacteria by catalyzing the reduction of alkyl hydroperoxide and hydrogen peroxide, the involvement of AhpC in cold adaptation of food spoilage bacteria was unclear. We introduced an expression plasmid containing ahpC into NBRC3832, which grows slower than NH04 at 10°C, and found that expression of AhpC enhanced growth. These results demonstrated that AhpC, which likely increases anti-oxidative capacity of LAB, plays an important role in their rapid growth at low temperatures.
Lactic acid bacteria (LAB) are useful for the production of fermented foods such as soy sauce and cheese (Caplice and Fitzgerald 1999; McKay and Baldwin 1990; Murooka and Yamshita 2008). Although the importance of LAB is widely recognized, LAB sometimes cause quality defects in fresh foods and fermented meat products stored at low temperatures (Borch et al. 1996). LAB that cause food spoilage can grow and cause quality defects by producing an unpleasant taste, white liquid, and a slimy substance (Asano et al. 2009; Hamasaki et al. 2003; Kondo and Ikeda 2000) even in cooked meat products such as ham and sausage stored at 10°C. Therefore, to ensure the safety of stored food and reduce such risks, elucidation of the cold-adaptation mechanism of LAB in food products is important. LAB that are closely related to Leuconostoc mesenteroides, Lactococcus lactis, Leuconostoc citreum, and Weissella viridescens grow rapidly at temperatures below 10°C (Borch et al. 1996; Chenoll et al. 2007; Diez et al. 2009; Hamasaki et al. 2003; Metaxopoulos et al. 2002; Samelis et al. 2006; Samelis et al. 1998), and are frequently isolated from spoiled cooked meat products stored at 10°C. These findings suggest a unique system of adaptation to low temperatures, causing rapid food spoilage in the refrigerator.
At low temperatures, microbes must overcome unfavorable conditions such as decreased membrane fluidity, molecular dynamics, and enzymatic activities. They can adapt to such an extreme environment via the induction of cold-shock and cold-acclimation proteins responsible for the maintenance of membrane fluidity, protein synthesis and folding, and metabolism (Feller and Gerday 2003; Graumann and Marahiel 1998; Marceau et al. 2004; Salotra et al. 1995; Wang et al. 2005; Wouters et al. 2000; Yamanaka et al. 1998). Identification and characterization of these proteins is necessary to understand the environmental adaptations of psychrotrophic LAB. Many studies on cold-inducible proteins have been reported, but few have focused on the relationship between these proteins and the growth of food spoilage bacteria. In order to prevent the contamination of foods by psychrotrophic LAB and to develop a method for their detection, we focused on the cold-adaptation mechanism of LAB at the molecular level. In this study, we characterized a novel psychrotrophic LAB, Leuconostoc mesenteroides NH04 (NH04), isolated from cooked meat stored at 10°C. L. mesenteroides was isolated from several spoiled cooked meat products at high frequency in our factories. NH04 grows rapidly at low temperatures within the shelf-life period and is responsible for the significant financial losses incurred. We analyzed the proteins that were inducibly expressed by this strain at low temperatures and identified a protein that facilitates the growth of L. mesenteroides at low temperatures.
Materials and methods
Bacterial strains, plasmids, and culture conditions
The strains used in this study are NH04 isolated from spoiled sausage and the related LAB, Leuconostoc mesenteroides NBRC3832 (NBRC3832) (NITE Biological Resource Center; Kisarazu, Japan). NH04 was deposited in NITE Biological Resource Center with accession number NBRC110676. For isolation of NH04, 25 g of spoiled product was suspended in 225 mL saline, and the suspension was subjected to 10-fold serial dilution. The dilutions (1 mL) were mixed with 15–20 mL BCP medium containing agar (Nissui Pharmaceutical Co., Ltd.; Tokyo, Japan), and the plates were incubated at 25°C for 72 h. The dominant species in the plates were isolated, and identified by sequencing their 16S rRNA genes. The nucleotide sequence of the 16S rRNA gene of NH04 was deposited in DDBJ with accession number LC005518. NH04 thus isolated and NBRC3832 were grown in 5 mL GAM broth (Nissui Pharmaceutical Co., Ltd.), with 1.0% d-glucose added to improve LAB growth. GAM broth consisted of peptone (10.0 g), soy peptone (3.0 g), proteose peptone (10.0 g), digested serum (13.5 g), yeast extract (5.0 g), meat extract (2.2 g), liver extract (1.3 g), dextrose (3.0 g), potassium dihydrogen phosphate (2.5 g), sodium chloride (3.0 g), soluble starch (5.0 g), l-cysteine hydrochloride (2.5 g), and sodium thioglycolate (0.3 g) dissolved in 1 L of deionized water (final pH of 7.1). The cells were grown at 10°C or 25°C in a compact rocking incubator (TVS062CA; ADVANTEC Toyo; Tokyo, Japan) by shaking at 70 rpm, and the growth was simultaneously monitored by measuring the turbidity at 660 nm with a spectrophotometer installed in the incubator. The kinetics of the growth was determined from three independent experiments. The LAB shuttle vector pGK::nucMCS (Le Loir et al. 1994) was used for the transformation of NBRC3832. Transformants harboring pGK::nucMCS and pGKahpC, which was constructed as described below, were cultivated in the presence of erythromycin (5 μg/mL).
Identification of cold-inducible proteins by two-dimensional electrophoresis (2DE)
Cells (10 mL) grown to stationary phase (1.5 < OD600 < 2.5) at 10°C and 25°C were harvested by centrifugation and resuspended in 100 μl of 50 mM Tris–HCl (pH 7.0). Cell suspensions were sonicated for 50 min at 10% amplitude (3 mm microtip, 400 W; Digital Sonifier; BRANSON Ultrasonics Corporation; Danbury, CT) while they were chilled on ice, and then centrifuged at 2,200 × g for 10 min at 4°C. Proteins in the supernatants were purified using the ReadyPrep 2-D Cleanup Kit (Bio-Rad Laboratories, Inc.; Hercules, CA) before an initial isoelectric focusing. Proteins (150 μg) were loaded onto Ready Strip IPG strips (17 cm, pH 4–7; Bio-Rad Laboratories, Inc.), and isoelectric focusing was performed using the PROTEAN IEF Cell (Bio-Rad Laboratories, Inc.), according to the manufacturer’s protocol. Treatment of the gel strips for 2DE was carried out as described previously (Kawamoto et al. 2007). After fixation and staining with SYPRO Ruby (Invitrogen Corp.; Carlsbad, CA), gels were scanned using Typhoon 9400 image analyzer (GE Healthcare Ltd.; Buckinghamshire, UK). Each experiment was performed three times to ensure reproducibility.
N-Terminal amino acid sequence of the protein inducibly produced at 10°C was determined using a PPSQ-21 protein sequencer (Shimadzu; Kyoto, Japan). A BLAST search (http://blast.ncbi.nlm.nih.gov/Blast.cgi) with the identified N-terminal sequence suggested that the cold-inducible protein was a homolog of AhpC. Degenerate primers for amplification of the ahpC gene of NH04 [peroxi130_For and peroxi569_Rev (Table 1)] were designed from conserved regions of the genes coding for AhpC from several LAB, including Leuconostoc, Lactobacillus, and Enterococcus species, and the internal nucleotide fragment was amplified. Based on the internal sequence of the gene coding for AhpC in NH04, an inverse PCR was performed with two primers (peroxi232_inv_For and peroxi407_inv_Rev) listed in Table 1. The sequences of the PCR products were determined and assembled to obtain the complete sequence of ahpC, which was deposited in DDBJ with accession number AB819067.
RNA extraction and quantitative real-time RT-PCR
Total RNA was extracted from cells cultivated at 10°C and 25°C using the RNeasy Kit (QIAGEN Inc., Valencia, CA). RNA pellets were dissolved in 0.1% diethyl pyrocarbonate-treated water and stored at −80°C until use. Quantitative real-time RT-PCR was performed with SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit (Invitrogen Corp.) and an Mx3000P Multiple Quantitative RT-PCR system (Stratagene; La Jolla, CA). The amount of mRNA in each sample was normalized with the amount of 16S rRNA. Each experiment was performed three times to ensure reproducibility.
Construction of AhpC-overexpressing strain
A gene fragment coding for AhpC was amplified using the primers peroxi_start_For and peroxi_end_SalI_Rev (Table 1), and the PCR product was fused with a DNA fragment containing a putative promoter region of the NH04 mannitol dehydrogenase gene (mdh) amplified by PCR with the primers thr_prm_XhoI_For and thr_prm_peroxi_Rev. The gene fragment was ligated with the pGK::nucMCS plasmid after digestion with XhoI and SalI. The constructed plasmid, pGKahpC, was introduced into NBRC3832 and NH04 by electroporation. Electroporation was performed using a previously described method with slight modifications (Leathers et al. 2004). Briefly, the cells from 50 mL culture were harvested at an OD600 of 0.6, washed twice with 75 mL of ice-cold deionized water, then washed once with 5 mL of ice-cold sterile electroporation buffer (1 mM of potassium phosphate buffer (pH 7.4) containing 1 mM of MgCl2 and 0.5 M of sucrose), and suspended in 1 mL of electroporation buffer. Plasmids (1 μg) were mixed with 40 μL of the cell suspensions and placed into a pre-chilled electroporation cuvette (0.2 cm). Electroporation was performed with the Gene Pulser Xcell PC system (Bio-Rad Laboratories, Inc.) at a capacitance of 25 μF. After electroporation, the cell suspensions were immediately diluted with 1 mL of GAM broth containing 1% d-glucose and incubated at 25°C for 2 h. Transformants were selected on GAM plates containing 1% d-glucose and 5 μg/mL of erythromycin.
Production of AhpC from NH04 was analyzed by SDS-PAGE. SDS-PAGE was performed on a 12.5% polyacrylamide gel (ePAGEL; ATTO Corp.; Tokyo, Japan). Soluble proteins (5 μg) were loaded and run on the gel. After fixation and staining with SYPRO Ruby, the gels were scanned using a Typhoon 9400 imager.
Growth of NH04
The growth of NH04 and NBRC3832 at 10°C and 25°C was monitored. NH04 grew faster than NBRC3832 at both 10°C and 25°C (Figure 1a, b). The doubling times of NH04 were 9 ± 0.6 h and 1.8 ± 0.1 h at 10°C and 25°C, respectively, whereas those of NBRC3832 were 13 ± 0.7 h and 2.5 ± 0.3 h at 10°C and 25°C, respectively. These results indicate that there were significant differences (p < 0.05, n = 3) between the doubling times of the two strains at both 10°C and 25°C.
Identification of the cold-inducible protein of cold-adapted Leuconostoc mesenteroides NH04
To identify the proteins contributing to the cold adaptation of NH04, we performed 2DE of the soluble proteins of NH04 grown at 10°C and 25°C. 2DE analysis showed that the amount of a specific protein, indicated by arrowhead in Figure 2a and b, was 2 ± 0.2 times higher (p < 0.05, n = 3) in the cells grown at 10°C than in the cells grown at 25°C. We further characterized this protein because it was one of the major proteins of NH04 and reproducibly more abundant at 10°C. The N-terminal amino acid sequence of this protein was MTTNFIDSEITDFKVNAYHD. The gene encoding this protein was cloned and sequenced as described in Materials and Methods, and it was found that this cold-inducible protein was a homolog of alkyl hydroperoxide reductase (AhpC). The ahpC gene (AB819067) coded for a protein of 189 amino acid residues, and the calculated molecular weight of the protein was 21,118.5. AhpC of NH04 shares a high sequence similarity (99.5% identity) with that of NBRC3832 (AB921977). Despite the high sequence similarity, the expression level of this protein was much higher in NH04 than in NBRC3832. 2DE analysis of the soluble proteins of NBRC3832 grown under the same conditions demonstrated that the fluorescence intensity of the spot for AhpC (arrowhead in Figure 2c) was approximately 17% ± 12% of that for NH04 (arrowhead in Figure 2a).
Transcriptional levels of ahpC in Leuconostoc species
The transcriptional level of ahpC in the NH04 cells grown at 10°C was approximately 3.8 ± 1.5-fold greater (p < 0.05, n = 3) than that in the cells grown at 25°C. The level of ahpC in NH04 grown at 10°C was approximately 2.8 ± 0.4-fold higher (p < 0.05, n = 3) than that in NBRC3832 grown under the same conditions.
AhpC facilitates the growth of LAB at low temperatures
To examine the effects of high-level expression of AhpC on the growth of LAB at low temperatures, we constructed an overexpression vector for ahpC (pGKahpC) and introduced it into NBRC3832, which grows slower than NH04. The level of AhpC in NBRC3832 harboring pGKahpC was approximately 6.5 ± 1.6-fold higher than that in the cells harboring a control plasmid, pGK::nucMCS (Figure 3a). NBRC3832 overexpressing ahpC grew more rapidly than the cells containing a control plasmid at 10°C (Figure 3b). The doubling time of NBRC3832 harboring pGKahpC was 15.4 ± 0.8 h, whereas that of the strain harboring pGK::nucMCS was 17.6 ± 1.1 h. The difference between the growth rates of NBRC3832 harboring pGKahpC and pGK::nucMCS was statistically significant (p < 0.05, n = 3). In contrast to the case of NBRC3832, the growth rate of NH04 was not affected by introduction of pGKahpC (Figure 3b). The doubling times of NH04 harboring pGKahpC and pGK::nucMCS were both 9.8 ± 0.3 h at 10°C. At 25°C, introduction of pGKahpC did not significantly affect the growth rate of NBRC3832 and NH04 (data not shown). The doubling times of NBRC3832 harboring pGKahpC and pGK::nucMCS were 3.0 ± 0.2 h and 3.2 ± 0.1 h, respectively, at 25°C, which were not significantly different from each other (p < 0.05, n = 3). The doubling times of NH04 harboring pGKahpC and pGK::nucMCS were both 2.0 ± 0.1 h at 25°C.
We isolated a novel food-spoilage bacterium, NH04, from spoiled meat product at 10°C, which causes food quality defects at low temperatures. Although little is known regarding the effects of various stresses on Leuconostoc species, they can survive in unfavorable environments such as oxidative and low temperature conditions (Con and Seamus 1986; Salotra et al. 1995; van de Guchte et al. 2002). Most Leuconostoc strains grow even at 10°C, and NH04 grows faster than other related strains at both 10°C and 25°C (Figure 1).
A comprehensive analysis of proteins synthesized in NH04 demonstrated that this strain inducibly produces a homolog of alkyl hydroperoxide reductase, AhpC, which likely has a role in anti-oxidative functions at low temperatures (Figure 2). AhpC homologs are widely distributed among prokaryotes, and AhpC shares approximately 40% amino acid sequence identity with thioredoxin peroxidase from yeast, rat, plant, amoebae, nematodes, rodents, and humans (Chen et al. 1998). AhpC homologs define a large family of anti-oxidants present in organisms from all kingdoms, and they protect cells from reactive oxygen species (ROS). ROS such as O2 −, •OH, and H2O2 cause oxidative damage to cells (Cabiscol et al. 2000). The O2 − generated during cell growth is typically converted to hydrogen peroxide spontaneously or by the activity of superoxide dismutase (SOD), from which the hydroxyl radical is generated by a metal-catalyzed redox reaction (the Fenton reaction). Hydroxyl radicals react immediately with cellular components and generate organic hydroperoxide (ROOH) that causes cell damage. AhpC catalyzes the reduction of organic hydroperoxide and protects cell components, thus acting as an anti-oxidant against hydroxyl radicals. Since cold stress induces production of ROS (Gocheva et al. 2009), psychrotrophic LAB that induce the expression of anti-oxidative protein(s) may grow rapidly at low temperatures. We demonstrated that the production of AhpC from NH04 in the related strain, NBRC3832, promotes growth at low temperatures (Figure 3). This suggests an important role for AhpC in the growth of LAB at low temperatures. Reduction of organic hydroperoxide may be crucial for LAB to grow at low temperatures. NH04 grows faster than NBRC3832 not only at 10°C but also at 25°C. However, the faster growth of NH04 at 25°C is not likely due to AhpC, because the expression level of AhpC in NH04 at 25°C is much lower than at 10°C and a statistically significant growth improvement of NBRC3832 by overexpression of AhpC was not observed at 25°C.
In this study, due to the lack of a gene disruption system for NH04 and related strains, the involvement of AhpC in the cold adaptation of NH04 could not be shown directly by a loss-of-function analysis. Nevertheless, the results obtained by heterologous expression of AhpC in a related strain strongly support the view that this protein is involved in the growth of LAB at low temperatures. Our results provide new insight into the molecular mechanism of cold adaptation of food-spoilage LAB that cause food quality defects during cold storage. In the future studies, we will examine whether AhpC is involved in the cold adaptation of other food-spoilage LAB, and whether other anti-oxidative substances play a role in cold adaptation. Our findings raise a possibility for the control of food-spoilage related LAB growing at low temperatures by developing an inhibitor against their anti-oxidation system. It may also be possible in the future for food manufacturers to detect food products and manufacturing equipment contaminated with food-spoilage LAB by developing a system to detect AhpC as a marker.
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This work was supported in part by Grants-in-Aid for Scientific Research (B) from JSPS (22404021, 24380047, and 25303028 to T.K.) and a Grant from the Japan Foundation for Applied Enzymology (to T.K.).
The authors declare that they have no competing interests.
SG designed the study, performed all the experiments, and wrote the manuscript. JK designed the study, helped the experiments, and wrote the manuscript. SBS helped experimental design and participated in the discussion on the interpretation of the results. TI, IW, and KK were committed to all the experiments. NE participated in the discussion on the interpretation of the results. TK designed the study and wrote the manuscript. All authors approved the final manuscript.
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Cite this article
Goto, S., Kawamoto, J., Sato, S.B. et al. Alkyl hydroperoxide reductase enhances the growth of Leuconostoc mesenteroides lactic acid bacteria at low temperatures. AMB Expr 5, 11 (2015). https://doi.org/10.1186/s13568-015-0098-3

domain: literature


This ain’t your granddad’s country music. Today’s country stars are just as at home on the pop charts as they are on the country tallies. Taylor Swift, Lady Antebellum, the Band Perry and Jason Aldean, among others, all made their mark in other genres in 2011.
Year In Music Main Page: See All The 2011 Charts
Swift, who was honored this month as Billboard’s Woman of the Year, made it a point to invite pop (and a few country) stars onstage during her Speak Now world tour, including Nicki Minaj, Flo Rida, Usher, Justin Bieber, Jason Mraz and Kenny Chesney. The Country Music Association (CMA) and Academy of Country Music both named her their entertainer of the year, and she picked up Billboard Music Awards to boot.
Inside The Year In Music:
Charts | Country | Latin | Pop | R&B Rock | Touring | Home | Buy Issue
@ Billboard.biz: Adele Cover Story
In our 2011 year-end recap, Swift scores the Top Country Artist nod and her 2010 release, “Speak Now,” leads the year-end Top Country Albums recap.
This was also quite a year for Lady Antebellum, the trio of Hillary Scott, Charles Kelley and Dave Haywood, which placed near the top in several categories, including its ranking as the Top Duo or Group of the Year (No. 3 overall). The threesome placed two of its Capitol Nashville albums in the top 10 of the Top Country Albums category: at No. 4 is Need You Now and at No. 6 is Own the Night, which topped the Billboard 200 in September after selling 347,000 copies, according to Nielsen SoundScan. The act ranks second on the Country Albums Artist list, behind Swift.
In November, Lady A picked up its third straight CMA Award for vocal group of the year, and earlier this year it nabbed five Grammy Awards and a Billboard Music Award.
If any act in country music had a breakthrough year, it was sibling trio the Band Perry. Not only did Kimberly, Reid and Neil Perry reach No. 1 on Hot Country Songs in late 2010 with “If I Die Young,” the song also hit No. 1 on Billboard’s Adult Contemporary chart. It tops the year-end Country Digital Songs list ( viewable on Billboard.biz ), and the act’s self-titled debut album finishes at No. 7 on the Top Country Albums recap.
Jason Aldean, who married country and rap on his hit “Dirt Road Anthem” is the top ranked male vocalist on the Top Artist list, coming in at No. 2 overall. He performed the tune at the 2011 CMT Music Awards with Ludacris, no less. Aldean’s duet with Kelly Clarkson, “Don’t You Wanna Stay,” was not only a country hit, but also charted on the Adult Contemporary Songs recap. The Georgia native is also gaining momentum on the touring front, recently winning the Breakthrough Award at the Billboard Touring Conference & Awards.
Blake Shelton may not have crossed over in the traditional sense, but he surely paved some pop culture in-roads in 2011 as one of four coaches on NBC’s “The Voice” alongside Maroon 5‘s Adam Levine, Christina Aguilera and Cee Lo Green. It was arguably his best year at country radio, scoring his fourth and fifth straight No. 1s on Hot Country Songs, a streak that dates back to 2009. It’s no surprise then that he leads the Hot Country Songs Artists recap for the year, placing “Honey Bee” at No. 8 on the Hot Country Songs recap.
The top spot on the year-end Hot Country Songs chart belongs to the Eli Young Band, which had the year’s biggest hit with “Crazy Girl.” It marked the first trip to the top of Hot Country Songs for the Texas foursome, which had previously failed to crack the top 10 in five attempts dating back to 2007.
The youth movement in country music continued in 2011. Among the top 10 on the Hot Country Songs Artists recap, the sole veteran is Chesney (No. 5 on the list), who scored his first Billboard top 10 airplay single in 1995. The rest of this recap roster all achieved their first hits in the past six years, including Aldean (2005), Swift (2006), Lady Antebellum (2007), Young (2009), Zac Brown Band (2008) and Bryan (2007). The trend is even more apparent on the year-end Top Country Albums Artists tally. There, Paisley (No. 7), who released his debut album in 1999, finds himself the senior member of a group that also includes this year’s “American Idol” winner, Scotty McCreery, who is No. 9.


text: Latest News The Vision Council Establishes New Optical Retail Division By Staff Friday, January 26, 2018 12:07 PM Mike Hundert, Vision Council chairman. SAN DIEGO—As optical executives gathered this week at The Vision Council’s annual Executive Summit here, the organization announced this morning that it has established a new Optical Retail Division. The event at the Hotel Del Coronado, held Jan. 24 to 26, draws executives from across the optical industry to hear about current economic trends and business strategies. “The relevance of retail’s role in the optical industry cannot be understated,” said Mike Hundert, CEO of De Rigo REM and chairman of The Vision Council’s board of directors. “As the industry has evolved, so has the relationship between retail and wholesale. The Optical Retail Division will enable The Vision Council and its members to stay at the forefront of how consumers experience and purchase optical products, and further our ability to empower better lives through better vision.”The 2018 Executive Summit has 35+ retail representatives from 16 different optical retailers in attendance, many of whom are existing members of The Vision Council through operating divisions such as the Lab Division. These companies, who have become the founding Optical Retail Division members, are able to maintain membership under each applicable division. Many of these representatives attended an inaugural Optical Retail Division meeting here, where they learned more about The Vision Council’s current programs and discussed potential future programs for the Division. The organization will continue to engage with these and other key leaders in optical retail in the development of programs and services, and is in the process of recruiting an Optical Retail Division liaison to further these relationships and best meet the needs of the newly announced Division. This meeting included representatives from MyEyeDr., Grand Vision/Insight Optical Company, Sam's Club Optical - Home Office, Vision Essentials by Kaiser Permanente, SCPMG, National Vision, Inc., Rosin Eyecare and Wal-Mart - Home Office.The annual meeting kicked off with The Vision Council’s inaugural “State of the Industry” address. Senior director of Industry Research Services, Steve Kodey, provided a U.S. optical market overview with the latest VisionWatch market research data that indicates the market is expected to grow by 1.8 percent in 2018. Each member of The Vision Council’s officers was confirmed for a second one-year term today during the summit’s business meeting portion, including Mike Hundert, chief executive officer and chief creative officer of De Rigo REM as chairman, Ken Bradley of Eschenbach Optik as vice chairman, Robert Liener of A&A Optical as the secretary/treasurer, and Marty Bassett of Walman Optical as immediate past chairman. Confirmed for a second two-year term as directors at large were Ray Khalil of Lafont USA, Don Oakley of VSP Optics Group, and Catherine Rauscher of Transitions. Rebecca Geifer of Modo Eyewear was confirmed for an initial two-year term as director at large. The complete roster for the 2018 board can be found online at thevisioncouncil.org.“The continued growth of our industry is dependent upon the personal growth and development of the executives who are moving the industry forward. It is an honor that our industry’s leaders trust The Vision Council, year after year, to provide the catalyst for that growth,” said Ashley Mills, CEO of The Vision Council. “I’m incredibly proud of the team of experts we have at our organization, including those presenting today’s State of the Industry, who are sought after by so many in the industry. With these resources, and the guidance of the optical leaders who make up our esteemed board of directors, I’m excited for what’s to come as we continue to strengthen our programs, our industry and the next generation of industry leaders.”More information about The Vision Council’s Optical Retail Division is posted at thevisioncouncil.org/opticalretail. Any questions about the new division, or information on becoming a member can be directed to Greg Chavez, The Vision Council’s executive vice president of operations and membership, at email@example.com or (703) 740-1399.

domain: news


1. Inform you about time table of exam.
2. Inform you about new question papers.
3. New video tutorials information.
When the length of a simple pendulum is decreased by 20 cm, the period changes by 10%. Find the original length of the pendulum.
Appears in these question papers
The period of a conical pendulum in terms of its length (l), semivertical angle (θ) and acceleration due to gravity (g) is:
If the metal bob of a simple pendulum is replaced by a wooden bob of the same size, then its time period will.....................
- remain same
- first increase and then decrease.
The phase difference between displacement and acceleration of a particle performing S.H.M. is _______.
(B) π rad
(C) 2π rad
Define an ideal simple pendulum. Show that, under certain · conditions, simple pendulum performs linear simple harmonic motion.
- Simple pendulum and Damped S.H.M
undefined video tutorial00:31:11


text:
- Artist: Otega
- Released: 2023
- Duration: 02:18
Otega – Maria Mp3 Audio Download
Nigerian music composer singer-songwriter and performer, Otega, introduces a song titled “Maria“.
The song “Maria” is a perfect addition to any music lover’s Playlist.
Otega‘s new song “Maria” is a must-listen for fans of music with relatable lyrics, emotive vocals, and catchy beat.
The impressive record serves as a track off the body of work Project, “Just Playing“.
You may download the music from the link below, and don’t forget to tell your friends about how much you love this track when you play it for them.
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Here in the state of Ohio, vehicle emissions testing is required for vehicles of all makes and models. If you own a car or truck here in Wadsworth, you’re subject to the same regulations. This is because vehicle exhaust severely affects air quality, and reducing those harmful emissions is one of the best ways to improve the air we all breathe. But don’t worry—emissions testing in Wadsworth, OH, is a fast and simple process when you bring your vehicle into WrenchWorks Truck & Auto Care!
The Importance of Emissions Testing
Vehicle emissions testing helps ensure that your car or truck is not releasing more pollutants into the environment than it should be. When a vehicle emits excessive amounts of pollutants, it can have serious consequences on local air quality. Even if your car only releases a small amount of pollutants above the legal limit, this can add up over time when hundreds or thousands of vehicles release high levels of pollution into the atmosphere.
Additionally, excessive emissions are actually a sign your car needs repairs! Symptoms of excessive emissions can affect fuel economy and cause your vehicle to run less efficiently, leading to additional engine wear and tear. By ensuring your vehicle meets local regulations with emissions testing in Wadsworth, OH, you’ll maximize the efficiency of your car or truck for a longer lifespan!
The Signs of Excessive Emissions
There are several signs that your car may be emitting excessive amounts of pollutants—but keep in mind that these symptoms can be caused by a variety of issues that we’ll need to adequately assess with our advanced automotive diagnostic technology! Some of these warning signs include:
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At WrenchWorks Truck & Auto Care, we offer comprehensive emission testing services for cars and trucks so you can stay compliant with Ohio regulations without hassle. And as a family-owned and operated auto shop in Wadsworth, OH, we pride ourselves on honest and high-quality work. So come on in today, and let us help you get your vehicle running clean! Additionally, you can schedule your appointment right now online!


text:
Will US Follow in Sweden’s Footsteps and Finally Admit It’s an ‘Actor’ in Ukraine?
19:44 GMT 03.11.2022 (Updated: 19:45 GMT 03.11.2022)
This week, Sweden’s top commander acknowledged that his nation is an “actor” in the Ukrainian security crisis, and said it was his job to prepare for “total defense” against an “armed attack” by, who else, Russia. Why have the Swedes been able to casually admit their involvement in Ukraine while the US and NATO haven’t? Sputnik explores.
Swedish Army Supreme Commander Micael Byden laid out a multi-year defense plan on Tuesday, announcing the Nordic nation’s plans to increase military spending to 2% of GDP by 2026, in line with NATO standards, and indicating that the military’s mission was “the total defense to prepare for what is the highest level of preparedness and armed attack.”
Perhaps most significantly, Byden admitted that by breaking its decades-old pledge of non-involvement in active military conflicts abroad and sending weapons to Kiev, Sweden had become an “actor” in the Ukrainian conflict, “at least in Russia’s eyes.”
Sweden reversed course on its ‘no offensive military aid’ policy this spring, sending over $100 million in assistance to Ukraine, ranging from anti-tank weapons, small arms, and personal protective equipment to air-to-ground missiles and artillery munitions. Swedish training assistance for Ukrainian servicemen actually began in 2018 under ‘Operation Unifier’, a training mission led by Canada.
3 November 2022, 08:44 GMT
Sweden’s Security Doctrine vs. NATO Doctrine
In his announcement, Byden didn’t openly name Russia as his country’s main enemy, but Sweden’s geography doesn’t leave much room for imagination, with Russia now the only one of Stockholm’s Baltic Sea neighbors that isn’t already a member of NATO or aspiring to join the alliance (with Sweden itself applying for bloc membership in May).
In that regard, Stockholm’s ambiguous position vis-à-vis Russia matches the one taken by NATO, which continues to maintain its “defensive” nature (despite being used repeatedly over decades past to attack Yugoslavia, Afghanistan and Libya), and to claim that the alliance has no aggressive plans against Russia. At its summit in Madrid this summer, NATO broke with decades of claims that it was not an “anti-Russian alliance” to openly state that Russia is “the most significant and direct threat to [alliance] security and to peace and stability in the Euro-Atlantic area,” but again assured that the bloc’s policy is “defensive.”
In a “Setting the Record Straight” “fact sheet” put out in September, the US-led military bloc emphasized that despite its training tens of thousands of Ukrainian troops, the sending of tens of billions of dollars in military equipment to Kiev and providing the Ukrainian military with intelligence support, NATO is “not at war with Russia” and does “not seek confrontation with Russia.”
Russian officials and independent observers have challenged such assertions, calling the Ukrainian crisis a “proxy” conflict against Russia, and accusing the West of waging a hybrid “total war” aimed at destroying, breaking up and strangling the country, pumping Kiev up with weaponry and coordinating Ukrainian missile and artillery attacks against Russian forces.
Why Can Sweden Say What NATO Can't?
Why has Sweden been willing to freely admit its role as an “actor” in Ukraine, while the US and most other NATO countries continue to stumble over themselves to assure that they are not directly involved?
Part of it has to do with pragmatism. So long as the US and its allies stick to their ‘not touching you, can’t get mad’ schoolyard bully tactics, Moscow’s warnings about the threat of escalation via weapons deliveries, the concentration of NATO forces on Russia’s borders, and other dangers stemming from the Western bloc’s approach will carry less weight in Washington and other Western capitals.
Instead, every time President Putin and other Russian officials warn of the risks of the Ukrainian crisis escalating or even threatening to go nuclear due to NATO’s actions, Western officials and media immediately begin churning out claims that Russia plans to nuke everyone to smithereens, prompting the Russian Foreign Ministry to issue statements encouraging everyone to calm down.
2 November 2022, 06:35 GMT
Another likely reason is economic. Although hundreds of Western companies have left the Russian market, and Brussels, Washington and London have taken steps to dramatically cut back reliance on Russian energy, remaining trade ties and dependence on key Russian resources prevent the West from cutting off Russia completely.
For example, while President Biden boldly announced US plans to ban Russian energy imports in March, he left out the fact that Russian uranium –the strategic resource powering America’s network of nuclear power plants, was nowhere to be found on the list of sanctioned energy resources.
The European Union has done the same thing, with Brussels’ latest anti-Russian sanctions package leaving some €210 million in Russian uranium off the list of sanctions.
19 October 2022, 10:52 GMT
Washington and Brussels obviously realize that cutting themselves off from these key resources will escalate the energy, inflation and economic crises pummeling their nations, and could even cause rolling blackouts in some areas. Not exactly a good scene for US politicians seeking reelection.
For Sweden, the economic argument may not be as big an issue, with trade with Russia collapsing by over 70 percent this year from nearly $5 billion in 2021, according to Observatory of Economic Complexity data.
Finally, the third reason the US and most NATO countries have sought to avoid admitting their involvement in a proxy conflict with Russia is politics. If more Americans or Europeans were aware of the existential nature of Russia’s security concerns in Ukraine, and the lengths President Putin and the Russian government are prepared to go to to avoid defeat, perhaps they would ask their governments more questions about their casual readiness to become further and further entrenched in the Ukrainian crisis and risking global catastrophe.


The company, which provides commercial data, analytics and insights for business, was acquired for $5.38bn last month by a consortium of private equity firms. Its data cloud holds 300 million business records.
“We’ve historically brought this strong credibility and trust, and in the past few years we’ve been hyper-focused on building out our presence online and figuring out how to apply that data and credibility in this environment,” Michael Bird, Dun & Bradstreet’s company’s executive VP and global GM, told Ad Exchanger.
Part of that process involves integrating its various tools to better serve customers and moving into new business areas.
“Where I see our road map going, for instance, is building on things like a recently launched product called DataVision that essentially is a customer data platform (CDP) for the B2B marketplace,” Bird explained.
“CDPs have been very active and an important part of the stack for consumer marketers, “but it hasn’t crossed the chasm to B2B and we’re squarely focused there,” he said.
Accordingly, the business is working on identity resolution – and Bird reported that “we are getting it to the point where we can say with confidence who’s who and who’s not in online media environments”.
Beyond that, Dun & Bradstreet is also building out analytics and segmentation, matching and integrating its data with cookie and ID pools to make it available to marketers.
“We’re moving closer to that targeting and then to the real-time media activation space,” Bird stated.
“Right now we’re filling out key partnerships and building the targeting tools, but it will eventually make sense for us to be more active there and own that capability,” he added.
Partnerships will remain a feature of its approach in other areas, however. “We’re not doing site design or dynamic content,” Bird pointed out. But “we can help inform what content and communications you display based on whether someone has professional buying power for your service, for example, or where they are in that B2B funnel.
“That’s going to help you get people to that sign-up or white paper or whatever conversion with fewer steps.”
Sourced from Ad Exchanger, Research Live; additional content by WARC staff


text:
The story shares the different immigrants that come to the U.S. for a dream. Whether it be our character Elena explaining the dreams her parents shared, to the people she has in her life. The story documents just some of the ways being an undocumented citizen is not an easy life, but it also shares their goals as to why they came as well.
My name is Anthony Correa. I am the second generation Chicano in my family. I am studying currently at Pioneer High School as a senior, about to graduate in the year 2016 in May. I am always interested in the lives of immigrants, because they understand what we have in life. At times, I am not grateful for what I have. Some immigrants give their perspective and what they have been through and it gives me a whole new perspective on what I have.

domain: sociology


text:
|Ambassador Liu Xinsheng met with Permanent Secretary of the Ministry of Agriculture of Cyprus|
H.E. Ambassador Liu Xinsheng met with Mrs. Egly Pantelakis, Permanent Secretary of the Ministry of Agriculture of Cyprus on May 28th, 2013. Mr. Fei Shengchao, Political Counsellor of the Chinese Embassy was present.
The two sides exchanged views on how to enhance agricultural cooperation between China and Cyprus, China and the European Union, and between China and the South European countries.

domain: agronomy


text:
As a nursing student or a nurse, you will know a lot about the noble profession. Nursing as you know is one of the most important professions today in the world which comprises a compassionate heart and scientific thinking. Like many people, they don't know some of the interesting facts about the nursing profession or nurses.
Each year, in the U.S Gallup, do ask adults to rate the ethics and honesty of some professions and for 18 years, Americans have been rating the nursing profession as the most ethical and honest profession.
The nursing profession has beat many other ethical and honest professions such as doctors, engineers, pharmacists, even clergymen.
This is very much true! A nurse can obtain a Doctorate of Philosophy in Nursing (Ph.D.) or a Doctorate in Nursing Practice (DNP) which is the highest degree available in nursing.
In the U.S there are over 3,059,800 nursing jobs according to the Bureau of Labor Statistics (BLS), and the nursing occupation is fast-growing and moving faster compared to other professions.
The famous essayist and poet Walt Whitman during the Civil War worked as a volunteer nurse. Some of Walt's works include The Wound Dresser and the Memoranda During the War which reflected his nursing experience during battlefield.
Although about 60 percent of nurses work in hospitals, some others work in different sectors of healthcare services such as outpatient care centers, doctors' offices, residential care communities, and nursing homes, educational services, and government agencies.
In 1878, Mary Eliza Mahoney enlisted in the nursing school which was operated by the New England Hospital for Women and Children. Only four completed the program out of the 42 students that enrolled for the program which Mary Eliza was among the four students
Many of the sanitation practices and hygiene used in modern nursing was introduced by Florence Nightingale. Also, she helped with the shape of the uniform now worn by nurses most especially the cap.
Nuns who cared for the sick for many decades wore veils and head coverings. During her lifetime, Florence's cap always sat on the top of her head which held her hair back.
A recent study shows that in a 12-hour shift, nurses walk about 4 to 5 miles. In comparison, an average American adult walks 2.5 miles to 3 miles in an 18-hour day.
According to a report from Medscape's Nurse Career Satisfaction Report 2018, it said that most nurses are satisfied with their nursing jobs. In the survey of about 10,284 nurses which include nurse practitioners, RNs, clinical nurse specialists, certified nurse anesthetists; they were asked to rate their job satisfaction. Most of them confirmed that they were happy with their jobs.
The World Health Organization designated the year 2020 as the year of Midwives and Nurses for the vital roles and their impact in providing health care. Also, it was noted that nurses are the first to care for the people in the community and they also face several challenging conditions.
More than ever, and at the present moment nurses are essential to the community and they are some of the most interesting people you can ever meet.

domain: medical


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Willow is my favorite witch! I am so happy how she turned out and am very pleased to offer her to you.
Willow measures 49 inches tall from the tip of her hat to the heels of her shoes.
I made Wilow from painted and stained muslin and sunbaked her for many hours. She is firmly stuffed and will hold her shape for many years to come.
Willow has needle-sculpted eyebrows, nose and mouth. Her eyes are vintage cream colored buttons. Her hair is dark brown sheep wool tips that I have hand-sewed to her head. Willow's 12 inch hat is made from painted grubby muslin. I tied a piece of aged cheesecloth to the brim.
Willow wears a sage green dress that has also been stained. Her matching shoes are painted, sanded and stained. Willow's bag is made from muslin and filled with sweet annie.
Willow will come to you with a grubby name tag. She is ready to bless your home for many, many years! Oh...and there is just a wee bit of "magic" in those shoes!!!


text: Sarcoidosis is a disease initiated by one or more unknown antigens in predisposed hosts, and causes noncaseating granulomatous inflammation. Uveitis is common and may affect any part of the eye. Protean systemic manifestations occur but pulmonary and cutaneous involvement is most common. Diagnosis is important; any suspicious uveitis should be investigated by relevant hematologic, radiologic, and invasive tests. A high proportion of patients require systemic corticosteroid or immunosuppressive treatment for uveitis. A significant minority become visually disabled because of macular scarring, glaucoma, or chorioretinal ischemia.


(ST. JOSEPH, Mo.) Revolution Lanes and Fun Center on the southside opened its bowling alley Wednesday night to help pay for the final expenses of Cody Grace.
"It’s really heartwarming to see all these people who cared," Caillyn McAlister, friend of Grace said.
Grace was one of the five family members killed in the tragic accident on Pear St., along with Amy Morse and her three young children.
Grace was the father of two of the three children, but friends said they were all family to him.
"He was an amazing father," McAlister said. "Every single one of those babies he was so absolutely excited for."
A bowling benefit was held in Grace’s honor at the bowling alley, complete with a silent auction and 50/50 raffle.
Organizers did their best to keep a happy atmosphere, but the emotional toll from the tragedy was felt from so many in attendance,
"I’ve been in tears, several times tonight," Gabriele Phillippe, asst. manager, Revolution Lanes and Fun Center said.
As tough as it is for friends of the family to heal, they said they take comfort in knowing Grace is with those who he loved the most.
"It’s a really sad ending for how it happened for Cody, but we’re glad you know that we can put him to rest with his family." McAlister said.
Over $700 was raised for Grace's funeral expenses by the bowling alley. Proceeds have been donated to the Rupp Funeral Home.
A GoFundMe page has also been set up to help pay for funeral expenses for Grace, a funeral service has been planned for Friday.


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Breaking bad habits early can help extend the life of your eyewear—saving you money and frustration. Dr. Specs Optical recommends that you avoid these habits when you’re wearing glasses—whether it’s your first pair or you’ve been wearing them for years.
Don’t clean them with tissue!
One of the biggest mistakes people make with their glasses is trying to clean their lenses with regular tissue like Kleenex. Tissue may feel soft to the touch, but for your lenses—it’s super rough! Regular cleaning with tissue causes tiny scratches on the surface of your lenses. Over time, those scratches can obscure and distort your vision when you’re wearing your glasses. To clean your eyewear properly, use a spray cleaner from your optician and a soft microfibre cloth.
Store them properly
Whenever you purchase a pair of glasses, they come with a storage case. For some people, the case sits at the bottom of a drawer and isn’t used day-to-day. But those cases are meant to be used to store your glasses after daily use. To make sure your glasses and sunglasses don’t get damaged, you should keep an eyeglass case anywhere that you take your glasses off—like your bedside table, office desk, car, or handbag. This helps ensure that your glasses stay protected when they’re not being worn.
Wear them carefully
Consider how many times you place your glasses on top of your head, or push hard on the nosepiece when your frames slide down your nose. Those simple actions can cause your frame to change shape and start to feel uncomfortable. This creates a vicious circle because the discomfort of a misaligned frame will cause you to keep adjusting it. Do your best to only wear your glasses around your ears, and use both hands at the temples to push your frame back up the bridge of your nose.
Explore Our Showroom
If your glasses are scratched, or the arms are bent, our team at Dr. Specs Optical can help you choose something new! We carry a wide selection of glasses and contact lenses and are conveniently located in central Kelowna beside the Orchard Mall. It might be a good time to have your eyes examined. Contact us today for more details about our hours and services.


DAYTON, Ohio (WDTN) – The headline act for the 2017 Vectren Dayton Air Show was announced Wednesday.
The U.S. Air Force Thunderbirds will perform at the annual event to be held in June at the Dayton International Airport.
The Air Show will hold its 43rd consecutive demonstrations June 24 and 25.
Air Show officials say the Thunderbirds will fly their six F-16 Fighting Falcon fighter jets at speeds up to 700 mph with the planes as close as 18-inches apart. 65 team members will travel to Dayton from Nellis Air Force Base in Nevada.
Three other acts were announced Wednesday as well.
The GEICO Skytypers team will perform a precision flying demonstration and create some unique aerial smoke messages.
Rob Holland Ultimate Air Shows will make its debut appearance in Dayton. Officials say his performance is new and a “game changer” on the air show circuit. The Vectren Dayton Air Show promises his aerobatic routine has never been seen in the Dayton skies.
Redline Airshow Aerobatic Team will return in 2017 as well.
“The Thunderbirds and three top civilian show performances is an awesome start to our 2017 show lineup,” stated Michael Emoff, Chairman of the United States Air & Trade Show Board of Trustees, the governing organization of the show. “We are thrilled that Dayton has the reputation to attract the very best in aviation and we are proud to make these announcements to our 2017 show. Fans are going to love it,” he added.
Air show officials say more attractions will be announced in the coming months. Tickets for the show will go on sale May 15, 2017.


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Harry Culverhouse FAQs: Facts, Rumors, Birthdate, Net Worth, Sexual Orientation and much more!
Drag and drop boxes to rearrange!
Who is Harry Culverhouse? Biography, gossip, facts?
Harry Culverhouse (born 29 October 1990) is a British actor born in London England. He is best known for his role in Dani's House as one of the main characters Cooordinator Zang. Ex Dani's House main character Toby was also played by Harry.
When is Harry Culverhouse's birthday?
Harry Culverhouse was born on the , which was a Monday. Harry Culverhouse will be turning 31 in only 5 days from today.
How old is Harry Culverhouse?
Harry Culverhouse is 30 years old. To be more precise (and nerdy), the current age as of right now is 10974 days or (even more geeky) 263376 hours. That's a lot of hours!
Are there any books, DVDs or other memorabilia of Harry Culverhouse? Is there a Harry Culverhouse action figure?
We would think so. You can find a collection of items related to Harry Culverhouse right here.
What is Harry Culverhouse's zodiac sign and horoscope?
Harry Culverhouse's zodiac sign is Scorpio.
♏The ruling planets of Scorpio are Mars and Pluto. Therefore, lucky days are Tuesdays and lucky numbers are: 9, 18, 27, 36, 45, 54, 63, 72, 81 and 90. Scarlet, Red and Rust are Harry Culverhouse's lucky colors. Typical positive character traits of Scorpio include: Determination, Self assurance, Appeal and Magnetism. Negative character traits could be: Possessiveness, Intolerance, Controlling behaviour and Craftiness.
Is Harry Culverhouse gay or straight?
Many people enjoy sharing rumors about the sexuality and sexual orientation of celebrities. We don't know for a fact whether Harry Culverhouse is gay, bisexual or straight. However, feel free to tell us what you think! Vote by clicking below.
0% of all voters think that Harry Culverhouse is gay (homosexual), 0% voted for straight (heterosexual), and 0% like to think that Harry Culverhouse is actually bisexual.
Is Harry Culverhouse still alive? Are there any death rumors?
Yes, as far as we know, Harry Culverhouse is still alive. We don't have any current information about Harry Culverhouse's health. However, being younger than 50, we hope that everything is ok.
Where was Harry Culverhouse born?
Harry Culverhouse was born in England, London, United Kingdom.
When did Harry Culverhouse's career start? How long ago was that?
Harry Culverhouse's career started in 2007. That is more than 14 years ago.
What is Harry Culverhouse doing now?
Supposedly, 2021 has been a busy year for Harry Culverhouse. However, we do not have any detailed information on what Harry Culverhouse is doing these days. Maybe you know more. Feel free to add the latest news, gossip, official contact information such as mangement phone number, cell phone number or email address, and your questions below.
Does Harry Culverhouse do drugs? Does Harry Culverhouse smoke cigarettes or weed?
It is no secret that many celebrities have been caught with illegal drugs in the past. Some even openly admit their drug usuage. Do you think that Harry Culverhouse does smoke cigarettes, weed or marijuhana? Or does Harry Culverhouse do steroids, coke or even stronger drugs such as heroin? Tell us your opinion below.
Are there any photos of Harry Culverhouse's hairstyle or shirtless?
There might be. But unfortunately we currently cannot access them from our system. We are working hard to fill that gap though, check back in tomorrow!
What is Harry Culverhouse's net worth in 2021? How much does Harry Culverhouse earn?
According to various sources, Harry Culverhouse's net worth has grown significantly in 2021. However, the numbers vary depending on the source. If you have current knowledge about Harry Culverhouse's net worth, please feel free to share the information below.

domain: celebrity


Azure Code Samples
Learn to interact with Azure services through code
22 code samples match your filter
Get started with the Adafruit Feather M0 Wifi Azure IoT Starter Kit
Get started with The Sparkfun Thingdev Azure IoT Starter Kit
Get started with the Adafruit Huzzah ESP8266 Azure IoT Starter Kit
UWP app connecting to Azure IoT Suite Remote Monitoring solution
Setup and connect an Intel Edision device to Azure IoT hub
Setup and connect a Feather M0 device to Azure IoT hub
This repo contains the source code to help you get familiar with Azure IoT using C language and Intel Edison board
Simple solution to test a device's connectivity to Azure IoT Hub: Device sends a message and receives it back.
This Python sample app is used by the getting started content to demonstrate how easy it is to create an IoTHub Instance on Azure portal, and register a new device for the IoTHub, then send a message from device (simulated device app) to cloud.
Simple cloud app that pulls data from public or private web service and pushes to event hub
Get started with the Raspberry Pi Azure IoT Starter Kit
Simple IoT project using Azure IoT Hub and showing a device running node to send telemetry data and that is analyzed by Azure IoT services to detect anomalies
A bridge that enables OMA LWM2M devices to interact with cloud back-end applications through Azure IoT Hub device twin and methods.


Population Connectivity Predicts Vulnerability to White-Nose Syndrome in the Chilean Myotis (Myotis Chiloensis) - A Genomics Approach
Copyright © 2020 Lilley et al. Despite its peculiar distribution, the biology of the southernmost bat species in the world, the Chilean myotis (Myotis chiloensis), has garnered little attention so far. The species has a north-south distribution of c. 2800 km, mostly on the eastern side of the Andes mountain range. Use of extended torpor occurs in the southernmost portion of the range, putting the species at risk of bat white-nose syndrome, a fungal disease responsible for massive population declines in North American bats. Here, we examined how geographic distance and topology would be reflected in the population structure of M. chiloensis along the majority of its range using a double digestion RAD-seq method. We sampled 66 individuals across the species range and discovered pronounced isolation-by-distance. Furthermore, and surprisingly, we found higher degrees of heterozygosity in the southernmost populations compared to the north. A coalescence analysis revealed that our populations may still not have reached secondary contact after the Last Glacial Maximum. As for the potential spread of pathogens, such as the fungus causing WNS, connectivity among populations was noticeably low, especially between the southern hibernatory populations in the Magallanes and Tierra del Fuego, and more northerly populations. This suggests the probability of geographic spread of the disease from the north through bat-to-bat contact to susceptible populations is low. The study presents a rare case of defined population structure in a bat species and warrants further research on the underlying factors contributing to this. See the graphical abstract here. https://doi.org/10.25387/g3.12173385.
Lilley, Thomas M.; Sävilammi, Tiina; Ossa, Gonzalo; Blomberg, Anna S.; Vasemägi, Anti; Yung, Veronica; Vendrami, David L.J.; and Johnson, Joseph S., "Population Connectivity Predicts Vulnerability to White-Nose Syndrome in the Chilean Myotis (Myotis Chiloensis) - A Genomics Approach" (2020). Biological Sciences Open Access Publications. 37.


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Your two great eyes will slay me suddenly;
Their beauty shakes me who was once serene;
Straight through my heart the wound is quick and keen.
I wish through life and after death you would be my queen.
Oohhhh… my my my..
She is so heavenly beautiful.
Follow Vishal Dutia on WordPress.com
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Another nail has been banged into USF1's coffin with reports claiming Jose Maria Lopez is on his way to Campos Meta.
Earlier this week, reports in Lopez's native Argentina claimed that USF1's attempted venture into Formula One had come to an end, culminating in team boss Peter Windsor informing Lopez that his services are no longer required.
Finances have been blamed for USF1's aborted start, with the team reputedly unable to raise necessary funds needed to compete despite the millions Lopez would bring.
Bad news for USF1 but, reportedly, good news for fellow financial strugglers Campos Meta.
According to AutoWeek, the team has jumped at the chance of signing Lopez, who along with his his father and advisor Felipe McGough met with Bernie Ecclestone on Thursday.
The F1 supremo played a huge role in hashing out a deal for the Argentine, who could be confirmed as a Campos driver as early as Friday.
"USF1 hadn't finished the car and we had promised everyone that we would be in the first race in Bahrain," a source close to Lopez told AutoWeek.
"That's why Felipe decided to reorganise everything, because we can't miss Bahrain. The only thing we want is that 'Pechito' drives in F1. Everything else is not important for us."
'USF1 on the verge of quitting F1'
Any reproduction, publication or redistribution of this material without the written agreement of 365 Media Group is strictly forbidden.


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Hottie Ian Somerhalder recently showed his support for his “stunning” ex-girlfriend, Nina Dobrev.
The Vampire Diaries co-stars reportedly dated for over three years. However, they ended the relationship back in May.
Yet, on September 9, Somerhalder showed his support for his former girl via Instagram. He posted an image of Dobrev’s recent Cosmopolitan cover, urging fans to go out and buy the magazine.
Dobrev finally opened up about her break-up with Somerhalder. She has explained that is was an amicable break-up that “was about finding what meshes and what doesn’t.”
The new season of The Vampire Diaries
will premiere on October 3 on The CW.
Published with Monogram


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This week I had the pleasure to attend Consensus 2018 in New York, the premiere gathering for the who’s who in blockchain, bitcoin and cryptocurrencies. Attendance doubled from last year to an estimated 8,500 people, all of them packed in a Hilton built for only 3,000. Ticket sales alone pulled in a whopping $17 million, while event booths—the largest of which belonged to Microsoft and IBM—generated untold millions more.
Market signals have been decidedly mixed thus far into the year. By this time a year ago, the S&P 500 had already returned 5%. Today, in early May, the S&P 500 is down fractionally, while volatility and bond yields are up.
The latest Conference Board Leading Economic Index (LEI) for April increased to 109.4 from a revised 109.0 in March. The Coincident Economic Index (CEI) came in at 103.5, up from the previous month.
Effective May 14, 2018, new regulations will be adopted aimed at increasing the transparency of bond pricing. The new rules require dealers of corporate, municipal and agency bonds to clearly disclose bond markups and provide retail investors with relevant price comparisons.
Global growth has been accelerating, but there are a few potential headwinds that could cause it to stall. Three of our senior investment leaders—Ed Perks, Chris Molumphy and Stephen Dover—recently participated in a panel discussion on the potential impact of trade tensions, inflation and other issues on their radar.
A review of last month’s market-moving events across countries and asset classes.
On May 14, new MSRB regulations will require the disclosure of the often dramatic markups that retail investors are subject to when buying individual municipal bonds. Will this accelerate the shift into active municipal bond management?
Call it the news of the year, perhaps even of the decade. For the first time since the Korean Peninsula was divided in 1948, leaders of the two warring nations met late last week in what had the look and feel of a jovial reconciliation between two estranged family members. Kim Jong-un of North Korea and President Moon Kae-in of South Korea made a number of important, though tentative, breakthroughs, including an agreement to denuclearize the peninsula and a pledge to revisit several infrastructure projects that would help bring some economic unity to the two Koreas.
When you see trade deficit data between the US and China, be aware that the numbers are vastly different depending on the source. China actually follows a more commonly used protocol to account for trade than does the US. For 2016, for example, US data shows a $375 billion trade deficit with China, whereas China shows $276 billion.
To address questions about the benefits of international investing and diversification, we don’t have to look too far back in time.

domain: finance


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Chesapeake Bay adult blue crab numbers hit record low, survey finds
The number of adult male blue crabs in the Chesapeake Bay has hit its lowest total since a formal dredging survey began in 1990.
The 2022 Baywide Blue Crab Winter Dredge Survey by the Maryland Department of Natural Resources reported Thursday the total abundance of blue crab in 2022 was 227 million crabs. The number of juvenile crabs in 2022 was 101 million crabs, a slight increase from 86 million juvenile crabs in 2021, but the third consecutive year of below average recruitment.
"Despite meeting these management objectives, adult blue crab abundance has steadily declined," the department said in a statement. "DNR will be working with partners in Virginia and the Potomac River Fisheries Commission on a coordinated approach to set management measures for the 2022 crabbing season that address conservation needs."
The department noted Chesapeake Bay jurisdictions have maintained harvest of blue crabs at safe levels established in the last stock assessment since 2008.
Behind the data
The number of spawning age female crabs has been above the management amount of 72.5 million crabs for eight consecutive years and at a level capable of producing a strong year class.
"The causes of some of these issues are outside our control as managers. There are environmental conditions and the changing climate among the number of factors," Michael Luisi, acting director of Fishing and Boating Services for the Maryland Department of Natural Resources.
Nonetheless, results showed there were 28 million adult male crabs, which is the lowest adult male abundance on record.
Among the often overlooked factors to a healthy yield of blue crab is healthy Bay grass for juvenile crabs that has seen a decline over the past three years.
"There are possible changes for the industry coming. They might be limited to their harvest and we may adjust the season, but that has yet to be determined," Luisi said.
Chris Moore, senior regional ecosystem scientist for the Chesapeake Bay Foundation, decried the trend as sign that not enough is being done to improve the bay's ecosystem.
More on Bay cleanup efforts:$238 million earmarked to restore the Chesapeake Bay. Here's how $40 million will be spent
More on rising prices for crabs:Here's why your Chesapeake Bay blue crab feast may be more expensive this summer
“The results of this year’s survey continue a worrying trend for blue crabs in the Chesapeake Bay region," Moore said. "The continued low abundance of juveniles and adult males indicates the urgent need for action to protect these segments of the population.”
He also said that although juvenile population estimates can vary greatly from year to year, the 2022 results are the third year of below-average numbers in this segment of the population.
These reduced abundances highlight the need to continue to protect adult females in order help ensure better numbers in the future, he said.
“This year’s survey follows two consecutive years of declines in the coverage of underwater grasses, one of the most important habitats for blue crabs in the Chesapeake Bay It is like," Moore said.
According to the environmental organization, water quality issues are also being exacerbated by invasive species like the blue catfish.
More on invasive species:Shrimp are migrating to Maryland waters. Will they find balance in blue crab domain?
The Winter Dredge Survey has been conducted cooperatively by the department and the Virginia Institute of Marine Science since 1990, and the results are reviewed annually in an effort to have consistent management efforts across the jurisdictions.
"We have a very good relationship with our management partners in the Chesapeake Bay. We're usually on the same page with our counterparts at the Virginia and the Potomac River Fisheries Commission," Luisi said.
Throughout the survey, biologists use dredge equipment to capture, measure, record and release blue crabs at 1,500 sites throughout the Chesapeake Bay from December through March.


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When filmmakers are looking for a fantasy landscape, New Zealand’s always top of the wish list.
Not only is the landscape diverse beyond imagining, the country’s compact and accessible, outdoorsy in all weathers and naturally designed for big adventures.
You could spend an entire family holiday on the amazing North Island beaches or hire a campervan and explore every national park, mountain range, marine reserve and colourful city. But, whichever way you ‘do’ New Zealand, it’s never short of phenomenal.
December to February is the New Zealand summer with temperatures between 20 and 32˚.
New Zealand has over 15,000km of coastline: from huge, sandy beaches in the far north of North Island to rugged shores trimming almost all round South Island.
Cinematic New Zealand’s mountain ranges, stunning glaciers, geysers, lakes, forests and enormous stretches of unspoiled wilderness are an amazingly accessible natural playground.
New Zealand’s fantastic for campervan holidays: not much larger than the UK, excellent roads and a countrywide network of campsites and parks on both North and South Island.
Auckland on North Island is New Zealand’s largest city and rated on the Mercer Scale as world’s third most liveable.
A variety of National Parks
New Zealand has 14 National Parks covering 30,000km². Tongarira on North Island is also a UNESCO World Heritage site.
Dramatic Otago Peninsula on South Island has the world’s only albatross breeding colony and a colony of rare Little Blue Penguins.
Roughly the size of England and Wales, South Island’s landscape is the mind’s eye New Zealand of staggeringly beautiful Alpine ridges, huge glaciers, fjords, dramatic coasts and vast, untamed national parks.
Christchurch is New Zealand’s third largest city. A good starting point for touring South Island’s east coast or as a base for whale watching at Kaikoura and step back in time days out at the pretty ‘French’ settlement of Akaroa.
Kaikoura’s famous for marine mammals and there are daily cruises year-round with almost guaranteed sightings of dolphins, fur seals and sperm whales, humpbacks, pilot whales and orcas.
Marlborough , north of Christchurch, is New Zealand wine country and South Island’s sunniest, most sheltered regions for walking and hiking, or sailing on Long Island-Kokomohua Marine Reserve.
Drive the Great West Road from Westport to Greymouth on South Island’s west coast. It’s 101km long and one of the world’s top 10 scenic journeys. The highlight’s Paparoa National Park and the Punakaiki Pancake Rocks.
North west coast towns to visit include: Greymouth, Franz Josef Glacier, Hokitika, Westport and Haast – centre of the Te Wāhipounamu World Heritage area.
Central Queenstown is the town at the heart of South Island’s most visited region. Stay here for outdoor adventuring round: Mount Aspiring National Park; Milford Sound; Lake Wakatipu; Glenorchy; and Gibbston Valley.
With names like Oban, Dunedin and Invercargill, it’s easy to spot the origin of South Island’s 19th century settlers – particularly on the impressive south west coast. This is the area for penguins and albatross on the Otago Peninsula; dramatic Tunnel Beach; and the intriguing Moeraki Boulders.
Dunedin Central is a great Victorian city, perched on New Zealand’s wildest coast. Famous for gothic architecture and grand design it’s also a UNESCO Creative City of Literature and home to several of the country’s leading writers and poets.
The smaller of New Zealand’s two main islands, North is the administrative and political heart of the country. Flawless, sun-drenched beaches and great swathes of rich farmland, fascinating cities, ancient Maori culture and some of the most remarkable geothermal landscapes in the southern hemisphere.
The harbour city of Wellington is New Zealand’s capital and mecca for Lord of the Ring fans – Peter Jackson’s Weta Studios are here and ‘real’ Middle Earth begins in earnest in the magnificent surrounding countryside.
Visit the Taraniki region, midway between Auckland and Wellington, for: the enormous volcanic cone of Mount Taranaki; legendary surfing beaches; dynamic contemporary art; great shopping; lively summer festival; and the amazingly accessible grandeur of Egmont National Park.
The Bay of Plenty on North Island’s east coast is exactly as described: plentiful. This is where kiwifruit grows, the country’s loveliest beaches string along the coast and a marine volcano adds more than a hint of drama to seaside, surf schools and dolphin cruises.
Rated the world’s third most liveable city and the largest in New Zealand, northerly Auckland’s great fun for kids whether they’re up for outdoor adventuring, theme parks, amazing museums or ziplining, swimming and exploring round the Hauraki Gulf.
Rotorua is the heartland of ancient Maori culture and tradition. But it’s also the region for geysers and bubbling mud pools, brilliant adventure parks, hot springs. Wild good times mixed with enthralling heritage are the themes here and it’s one of North Island’s most exciting areas for family holidays.
What to do
Middle Earth Adventures, North and South Island
Fantasy expeditions range from the chance to Hobbit about for a few days to several weeks of full Lord of the Rings’ questing.
Fjordland National Park, South Island
Arguably the most impressive of New Zealand’s diverse and dramatic national parks, Fjordland is in the far south west of South Island.
Skyline Rotorua, North Island
Ziplining, luging, racing, sky-swinging or stargazing, Skyline’s wild adventure for all ages in Rotorua, just over an hour from Bay of Plenty.
Whitewater Rafting, North Island
Thrilling family rafting on the tallest commercially ridden waterfalls in New Zealand including Kaituna on the Okere River.
Adventure Forest, North Island
Within driving distance of Auckland, Adventure Forest takes what comes naturally to New Zealand and adds vast treetop walks, pulleys, lines, swings and wild family fun.
Whale Watching, South Island
Kiakoura, north of Christchurch, is the undisputed centre of whale and dolphin watching in New Zealand.
Sea Kayaking, South Island
Penguin colonies, fur seals and Pohatu Marine Reserve’s spectacular seascape make kayaking with local conservationists an unforgettable family adventure.
Egmont National Park, North Island
Centred around the perfect volcanic cone of Mount Taranaki, Egmont National Park is wonderful for walking and hiking in summer and winter skiing.
Museum of New Zealand, Wellington
Put aside at least an afternoon to explore this vast interactive museum which also has an innovative kid’s event programme throughout the year.
Weekend Treasure Quest, North Island
Built over a century ago, the town of Waihi is famous for gold, silver and fortune hunters. Today its unusual heritage makes a fascinating family weekend of discovery and beachy fun.
Educational value for kids
New Zealand’s world famous for glacier climbing. Fox Glacier and Franz Josef (west coast, South Island) are easily accessible for families, with or without guides.
Ride New Zealand’s only narrow-gauge railway in the Coromandel Forest and learn the fascinating story of Driving Creek
The Whale Station Experience on South Island is a fascinating in-depth exploration of the 19th century whaling industry and today’s conservation work.
Mount Hikurangi is at the heart of ancient Maori culture and guided treks and walks are fascinating for older kids.
Any one of New Zealand’s national parks is a learning experience with kids and almost all are easily accessible and have excellent visitor and outdoor activity centres.
Almost all public museums and art galleries in New Zealand are free of charge or have nominal ticket prices.
Take at least one eco-tour in New Zealand and discover how this remarkable country is protecting and conserving its natural heritage.
Getting about with kids in New Zealand
Visitors to New Zealand are always surprised by how easy it is to explore. Hire a campervan or car on either North or South Island and there are hundreds of itineraries and well-marked routes designed for touring. Public transport’s excellent and extensive in cities and North and South Island are connected by daily ferry services from Wellington or flights from Wellington to Christchurch. Be aware of fuel if you’re driving in more remote areas, filling stations are few and far between.

domain: travel