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Venavine Leg Vein Health 30 Capsules with red vine leaf extract provides relief for heavy, tired, painful and swollen legs by strengthening leg vein walls.
Venavine Leg Vein Health 30 Capsules
Common symptoms associated with poor leg vein health:# Tired, heavy and swollen legs, especially in the evening. Sensation of pain and tension in the calves after standing or walking for long periods. Tingling sensation in the legs. Cramps in the muscles, especially at night. Itching and hardening of the skin on the lower leg. Red vine leaf extract can have the following effects:* Protects, seals and strengthens the walls of the leg veins and capillaries. Improves blood circulation in the legs. Reduces fluid loss into the tissue around the veins. Alleviates discomfort of tired, painful and swollen legs. Reduces painful night time leg muscle cramps. #For a diagnosis or if symptoms persist, consult a medical practitioner. *Efficacy of support may vary between users. D.7.6 vascular medicine - other. Western herbal medicine. Tel: +27 (0) 12 664 7110. Fax: +27 (0) 12 664 8031. Customer care line: 0860 Nativa (628 482). E-mail: [email protected] Website: www.venavine.co.za.
Quantity in pack:
Venavine®. Capsules. Leg vein health. With red vine leaf extract. Supports the body with:* Heavy, tired, painful and swollen legs. Strengthening of leg vein walls. New one-a-day capsule! 30 capsules.
Dosage and directions for use: Do not exceed the recommended daily dosage. Adults 18 years and older: Take 1 capsule, daily after breakfast, with a glass of water.
Store below 25 degrees C. Store away from moisture and direct sunlight. Replace cap firmly after use.
Keep out of reach of children. Do not use if known hypersensitivity or allergy exists towards any of the ingredients. If in doubt consult your medical practitioner. Products containing herbal ingredients may interact with some medication. If you are taking any chronic medication, do not use this product without consulting your medical practitioner. Do not use after date of expiry. Check expiry date on unit. Refer to package insert for more information. This medicine has not been evaluated by the Medicines Control Council. This medicine is not intended to diagnose, treat, cure or prevent any disease.


FORMER PBA import Vernon Macklin is in town as his current team, the Changwon LG Sakers, are gearing up for the coming season of the Korean Basketball League (KBL) with a series of tuneup games against PBA games.
Macklin last played in the country for Magnolia in the 2018 Commissioner’s Cup, but he is best remembered for his stint with Barangay Ginebra in the 2013 Commissioner’s Cup where he led the Kings to a runner-up finish under Alfrancis Chua.
On Tuesday night, the 32-year-old Georgetown and Florida product saw action for the Sakers in an exhibition game against Barangay Ginebra with his former team winning the tightly-contested match, 93-91.
Macklin said it was only the second time he was able to face the Kings since his stint with the popular team in the 2013 Commissioner’s Cup. The first came on Friday when the two teams squared off in a separate tune-up game.
The high-flying import said he was thrilled to renew acquaintances with the remnants of the 2013 team in LA Tenorio and Mark Caguioa.
“It was my first time since I left the PBA,” said Macklin. “It was pretty cool. I’ve made (friends) with a lot of the guys. I love being here in the Philippines. It’s pretty cool.”
“We scrimmaged against them a few days ago. I was able to talk to LA (Tenorio), Mark (Caguioa), Justin (Brownlee), Stanley (Pringle),” said Macklin.
Macklin said the Kings are definitely a different team now compared to the 2013 squad, pointing out that the current squad is now much bigger thanks to twin towers Greg Slaughter and Japeth Aguilar.
“They have two bigs now with Japeth and Greg so they can go with Justin in the three-spot. They have Stanley and coach Cone is pretty good as well,” said Macklin.


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SOCIETY FOR CAPITAL MARKET RESEARCH & DEVELOPMENT
ORIGIN AND PURPOSE
The Society for Capital Market Research & Development (SCMRD) is a
non-government not-for-profit tax-exempt research body. It was registered on
4th October, 1989 at Delhi under the Societies Registration Act.
(Registration No. S / 20422)
Its creation proved very timely and was widely welcomed in official,
financial and academic circles. The Ministry of Finance, Government of
India, as well as the SEBI entrusted it with certain important studies.
The Department of Scientific & Industrial Research, Government of
India, has recognised it for tax exemption to donors u/s 35(1) (iii).
Its mission has been to promote the development of the capital market and protection
"THINK TANK" for CAPITAL MARKET DEVELOPMENT
The Society acts as an independent "Think Tank". It develops
research ideas in consultation and collaboration with the regulatory and
operating organisations. This ensures that its research is made to bear on
An outstanding example of the contribution made by the Society's studies
is that of its 1991-study, entitled Stock Exchange Trading in India : Agenda for
Reform. The revolutionary idea of creating the National Stock Exchange
arose out of this study. The Pherwani Committee, on which the Society was
represented through its Director, recommended the idea and it was
followed up by the Society at the Finance Ministry level.
The Society has throughout been playing a very
crucial role in the reform and development of the Indian capital market.

domain: finance


100% Certified Organic Cotton
All our garments are lined with 100% organic cotton. The production and processing of organic cotton delivers proven benefits for people and the environment. Cotton is soft, comfortable and the result is cotton with stronger fibers that last longer thereby prolonging the life of our garments. Most importantly allow the most important part of your functioning and healing body to breath.
LYCRA® fabrics are designed to improve the effectiveness of shapewear. The stretch and recovery capabilities of LYCRA® have been chosen for the shaping components required in both Essential Collections to offer durability and shape retention for sculpting, smoothing, comfort, style quality, and confidence.
Ultra-Fresh antimicrobial treatments inhibit the growth of bacteria responsible for creating unpleasant smells and staining in your garment, a valid concern experienced by nursing and recovering women. By controlling unwanted microbes, Ultra-Fresh antimicrobials help to keep your garment fresh, hygienic and odor free ~ maintaining product quality and extending the life of the fabrics.
Moisture Wicking Treatment
The shedding of excess water post birth leads to increased sweating; a common experience shared by new mothers. Moisture wicking treatment has been incorporated into the Empress Collection to speed up evaporation and drying of fabric. This feature pulls the dampness from your body to the surface of your garment. The result is a high performance feature that keeps you dry and comfortably throughout your day.
Power mesh is incorporated into our designs to provide ventilation while also offering support and compression where needed. It's lightweight with four-way stretch making this fabric strong, flexible and shapely.
Our use of Stretch lace not only adds a hint of sensuality to our garments. It is a functional fabric that offers you four-way expansion to compliment your transitioning body and accommodate you through any stage whether it be maternity, nursing, postpartum or just a treat for everyday!


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Easy Vegetarian Lentil Meatballs - Simple, healthy and protein packed! Made with lentils, carrots, and Italian spices, then oven baked. Perfect for filling meatless meals, and they taste great leftover too!
Ingredients:FOR THE MEATBALLS:
- 3/4 cup dried green — brown, or French lentils (I used green French lentils)
- 1 1/2 cups low-sodium vegetable broth — or chicken broth, plus additional as needed
- 2 teaspoons olive oil
- 1/2 cup diced yellow onion — about 1/2 medium onion
- 1 cup shredded carrots
- 2 cloves garlic — minced (about 2 teaspoons)
- 1/2 cup old-fashioned rolled oats — or quick-cooking oats, do not use instant or steel cut
- 1/4 cup chopped fresh Italian parsley
- 1 1/2 tablespoons tomato paste
- 1 teaspoon dried oregano
- 1/2 teaspoon kosher salt
- 1/4 teaspoon black pepper
- 1 large egg
- Prepared whole wheat pasta noodles — zucchini noodles, or sweet potato noodles
- Good-quality red pasta sauce — store bought or homemade or pesto
- Rinse the lentils: Measure the lentils into a colander or strainer. Pick over and remove any shriveled lentils or small pieces of rock or other debris. Rinse well under cool water. Drain.
- Cook the lentils: Add the rinsed lentils to a medium saucepan with the vegetable broth. Bring to a rapid simmer over medium high, then reduce the heat to a very low simmer. There should be a few small bubbles, and the lentils should be barely moving. Let gently simmer for 20 to 30 minutes, until the lentils are tender. Keep an eye on them to ensure they do not dry out—you want the lentils to always be just barely covered with liquid. If the lentils are not yet tender but the liquid has been has been absorbed, add more water and continue to cook. Drain off any excess liquid and set aside.
- Meanwhile, heat the olive oil in a medium skillet over medium-low heat. Once hot, add the onion and cook until onion is translucent and lightly brown, 6 to 8 minutes. Stir in the carrots and cook for another 2 minutes, then stir in the garlic and cook until fragrant, about 1 additional minute.
- Place the oats and parsley in the bottom of a food processor fitted with a steel blade, then pulse a few times to begin breaking up the oats. Add the cooked lentils, onion mixture, tomato paste, oregano, salt, and pepper. Pulse a few times to start combining the mixture, then crack in the egg. Pulse a few more times until the mixture is combined but the lentils still have some texture. Set aside and let rest for 10 minutes or refrigerate overnight.
- Preheat the oven to 425 degrees F. Line a large rimmed baking sheet with parchment paper, then coat the paper with nonstick spray. Roll the lentil mixture into balls that are roughly 1 1/2 inches across, about the size of a golf ball. Arrange them in a single layer on the baking sheet and mist the tops with nonstick spray. Cook for 10 minutes, then turn, mist again with nonstick spray, and continue baking 8 to 10 additional minutes, until the meatballs are browned and lightly crisp. Serve warm with pasta or vegetable noodles and your desired sauce.
- To make this recipe vegan, you can use a flax egg in place of the egg called for in the recipe.
- Make-ahead options: Unshaped lentil "batter" or shaped, unbaked lentil balls can be stored in the refrigerator 1 day in advance. Bake (or shape and bake) as directed. You can also freeze the shaped balls unbaked or baked for 2 months, then thaw overnight in the refrigerator. Either cook as directed (if unbaked) or rewarm gently in the microwave (if baked).
- Store leftovers in the refrigerator for up to 4 days. Reheat gently in the microwave.

domain: food


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Despite last week’s drop in interest rates, which many lenders have passed on in their variable rates for mortgage offers, and the increasing drop in property prices, many first time buyers are still finding it difficult to get on the property ladder. The reason for this is that most mortgage deals available to borrowers require a sizable deposit.
Figures released by Moneyfacts recently showed that on thirty-five mortgage deals available required a 5% purchase price deposit, and just sixty-six mortgage deals were available with a 10% deposit. The means that first time buyers are having to scrimp and save in order to be able to afford a property, even at the lower end of the pricing scale.
Last year there were 1,126 mortgage deals available with deposits of 5% and over.
The Council of Mortgage Lenders says that first time buyers are being discriminated against because lenders see them as being a high risk of going into arrears, and many first time buyers are having to borrow additional funds from parents in order to secure a mortgage deal.
Borrowers with high deposits however have more options open to them now than a year ago, as there are 228 mortgage deals available to borrowers with a 15% deposit, whereas there were only 198 available last year.
With interest rates expected to fall again next year, borrowers are keen to take advantage of the situation and take out tracker mortgages where possible, however many of the tracker mortgage deals are only available with very deposits, 25% and above.
HSBC is currently offering a tracker deal at 0.99% above the base rate, so long as the borrower has a 40% deposit. This is out of the reach of most first time buyers.

domain: news


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Mount Kimbie: We Go Home (Feat. James Blake)
James Blake and Mount Kimbie showcased their combined genius recently and I couldn’t be happier. Well, I could be; the song’s too bloody short! This is a collaboration that I’ve long-desired but it feels as though we’re being teased with a preview. I don’t particularly believe in heaven or hell anymore, but I assume that the latter is a world filled with beautiful James Blake inspired songs that end at the two minute mark —right as your body begins to be engulfed by the euphoria created by the artist. Oh, the cruelty!
Nonetheless, “We Go Home Together” is a work of art that deserves appreciation for what it is. Hats off to the trio.

domain: entertainment


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Latin America: Economic Snapshot for Latin America
September 12, 2018
Recovery stays on track in Q2 but headwinds to growth mount in Q3
Preliminary data revealed that growth held up in Latin America’s economy in the second quarter, despite global geopolitical uncertainty and political tensions in key economies. Regional GDP (excluding Venezuela) grew 1.9% over the same period last year in Q2, matching Q1’s result. Tailwinds from higher commodity prices, recovering private consumption and a solid global economy supported growth in the quarter. However, growth was uneven across countries and activity in the more vulnerable economies of Argentina and Brazil floundered.
Looking at the individual economies with GDP data available, economic dynamics strengthened in Chile, Colombia, Mexico and Peru in the second quarter. Chile and Peru were the region’s star performers with growth hitting multi-year highs thanks to surging investment and solid household spending. Similarly, the domestic economy drove growth in Colombia aided by rising economic sentiment. While a healthy services sector and rebound in industrial output supported Mexico’s economy—which held up well in the face of tough NAFTA talks and uncertainty related to the 1 July general election—growth still disappointed somewhat, landing shy of expectations.
In contrast, the region’s largest economy, Brazil, lost momentum in the second quarter. A nationwide truckers’ strike that began in May disrupted economic activity, as protests broke out over diesel price hikes which were introduced to help improve the government’s finances. Exports plunged as goods were unable to reach the nation’s ports, while falling consumer confidence weighed on household spending. Meanwhile, while official Q2 data is not available yet, Argentina’s economy is expected to have swung to contraction due a prolonged drought and financial crisis, which saw the peso plummet and interest rates soar.
More recently, headwinds have picked up in the third quarter, preventing a stronger recovery. Tightening global financial conditions and contagion from Turkey’s crisis caused a broad selloff in many of the region’s currencies, which will fan price pressures going forward. In particular, the selloff exacerbated Argentina’s already ongoing financial storm, and the peso plunged around 20% in just two days in August. In response, the Central Bank raised rates to 60%—the highest in the world—and President Mauricio Macri called on the IMF to accelerate disbursement under the country’s program. However, it remains to be seen if the measures will be enough to stem the peso’s slide and the country’s economic situation is still precarious.
Venezuela’s economy is also in a challenging situation and GDP is expected to remain in freefall due to plunging oil output, hyperinflation and shortages of basic goods. In an attempt to rein in the crisis, the government unveiled a raft of new measures in August including a new currency, the sovereign bolivar and minimum wage hikes. The government plans to anchor the currency to the petro, the country’s cryptocurrency, a move which is generating confusion. The reforms are unlikely to steady the economy, however, as they fail to tackle the fundamental issues of foreign exchange misalignments and chronic mismanagement.
Meanwhile, political risks are rising in Brazil, the region’s largest economy. The country heads to the polls on 7 October to decide the next president, and the outcome is highly uncertain, with a second-round presidential vote practically guaranteed. The clear front-runner in polling, former President Luiz Inácio da Silva (more commonly known simply as Lula) has been banned from the race and several candidates have a shot at making it to the runoff vote. Irrespective of who succeeds the current president, Michel Temer, the next president will face a tough challenge of trying to stoke a faster recovery, reduce bloated government accounts and navigate a likely divided Congress to force through legislation. So far centrist and reformist candidates are performing poorly in the polls.
Argentina’s downward spiral dents Latin America outlook
Latin America’s 2018 growth prospects were downgraded this month for the second consecutive publication. The sizable downgrade to Argentina’s GDP forecast, as the country remains mired in a financial crisis, was the main driver behind the cut. Elevated inflation, drought and tough IMF-demanded austerity are expected to cause the country’s economy to contract this year and the crisis will also likely hurt its main trading partners through lower export demand. Outside of Argentina, tighter global financial conditions are also weighing on the region’s growth outlook as is political uncertainty in Brazil. On a positive note, NAFTA-related risks to Mexico’s economy have subsided somewhat, after the country struck a preliminary deal with the United States in August.
Regional GDP (excluding Venezuela) is now seen growing 1.9% this year, down 0.1 percentage points from last month’s forecast. Next year, regional growth is seen picking up pace and coming in at 2.6%. This month, Argentina and Brazil had their forecasts cut, along with Uruguay. In contrast, Bolivia, Chile, Colombia and Peru had their projections raised after positive Q2 GDP readings, while the rest of the region’s economies saw no changes to their forecasts.
Given the current economic conditions in Venezuela and the limited availability of official data, it has become extremely difficult to forecast the country’s economy. We have therefore removed Venezuela from the regional aggregates and discontinued its long-term forecasts. Rampant inflation, dwindling oil production and a dysfunctional exchange rate system are expected to keep Venezuela in a dire economic crisis, and the economy is seen contracting 12.1% this year, down 0.8 percentage points from last month’s forecast, and 4.2% in 2019.
BRAZIL | Elections take center stage, while economic data remains soft
GDP data confirmed that the recovery stuttered in the second quarter of the year, weighed on by economic disruptions from the truckers’ strike. Exports plunged due to transit disruptions, while household spending also slid in Q2, likely weighed on by deteriorating sentiment and a spike in inflation. More recent data for the third quarter continued to be downbeat, suggesting that a strong recovery has yet to materialize after the worst recession in the country’s modern history. Business and consumer confidence both fell in August amid a tense and uncertain political backdrop, and industrial production contracted in July. Moreover, the real plunged in recent weeks, boding poorly for inflationary pressures. Against a challenging economic backdrop, the country will head to the polls on 7 October in presidential and legislative elections. The battle for president remains crowded with a second-round vote nearly guaranteed. A centrist and reform-minded president is badly needed to navigate Brazil’s congress to pass tough reforms; however, Geraldo Alckmin, so far the most market-friendly candidate, is polling in fifth place.
Brazil’s prospects were downgraded for the fourth month in a row following a modest Q2 GDP outturn. In addition, a less supportive global backdrop and the weakening of the real cloud the country’s outlook. A market-friendly outcome to the election remains crucial to ensuring a sustainable recovery. FocusEconomics panelists now see the economy growing 1.6% this year, down 0.1 percentage points from last month’s forecast. Next year, GDP is seen growing 2.5%.
MEXICO | Government strikes trade deal with U.S.
Negotiators breathed a cautious sigh of relief in late August as they announced that Mexico and the United States had struck a deal to update NAFTA. Although the plan left the fate of Canada—the pact’s third partner country—in limbo, it calmed markets that had begun to fear the inevitable dissolution of the quarter-century-old agreement. Details of the bilateral talks suggest significant concessions by Mexico in the automotive sector, and early analyses of the tentative deal offered diverging views on its merits. The announcement came on the heels of revised second-quarter national accounts data, which confirmed weak first estimates held back by downbeat agricultural and industrial readings. Meanwhile, available data for the third quarter hints that a nascent domestically-driven turnaround could be afoot. In the wake of AMLO’s landslide victory on 1 July, economic sentiment has been riding high. That said, the manufacturing sector has yet to fully bounce back, and trade data in recent months has been far from encouraging.
Tight domestic and U.S. job markets, as well as improved private-sector lending, are expected to support household spending this year. Diminishing political uncertainty, meanwhile, should buoy investment. Although external trade remains a concern, healthy factory output in the U.S. is seen bolstering manufacturing exports, while a clearer path forward for NAFTA should keep supply chains intact. In politics, most analysts expect AMLO to govern as a big-spending centrist. FocusEconomics panelists expect growth of 2.2% in 2018, unchanged from last month’s estimate. For 2019, panelists see growth stable at 2.2%.
ARGENTINA | Macri’s plea to IMF triggers further turmoil
President Mauricio Macri’s plea to the IMF on 29 August triggered a sharp selloff in the peso, precipitating the worst chapter thus far of this year’s still-unfolding economic crisis. Macri appealed to the IMF to accelerate the disbursements of its USD 50 billion stand-by arrangement (SBA) agreed to in June, a move that sent investors fleeing as the depths of the government’s fiscal woes grew more apparent. In exchange for bringing forward the disbursements, the government offered new fiscal reforms intended to speed up consolidation. Ahead of the IMF’s announcement on a revised SBA, the pain facing the economy continues to mount. Confidence is in the doldrums and tighter monetary conditions are bound to bruise spending and investment this quarter. Exacerbating matters, economic activity contracted in the second quarter as drought wreaked havoc on the agricultural sector, and a brewing corruption scandal in the construction sector is poised to weigh on activity through the remainder of the year.
Amid the hobbling of activity and the worsening of the crisis, the economy has likely entered recession and a majority of FocusEconomics Consensus Forecast analysts now see the economy contracting this year. That said, a number of analysts are still taking recent events into account. Nevertheless, spending and investment metrics are expected to plummet as oppressive financing conditions and elevated inflation take hold in the aftermath of the peso’s freefall. LatinFocus Consensus Forecast analysts see the economy contracting 1.2% this year, down 1.6 percentage points from last month’s forecast, before returning to feeble growth next year at 0.7% in 2019.
COLOMBIA | Domestic demand nudges up Q2 growth
The latest national accounts data showed that the economy accelerated in the second quarter on the back of a rise in domestic demand. Private consumption picked up pace as inflation dipped and consumer confidence jumped to a three-year high, while fixed investment rebounded on a recovery in machinery and equipment. Although exports rebounded in Q2 on higher oil prices, the external sector dragged more severely on growth as imports outpaced exports. Going into the third quarter, private consumption likely moderated as the unemployment rate crept back up in July and consumer confidence fell in the month. July’s PMI reading was higher than the previous month, however, signalling improved prospects for the manufacturing sector. On 27 August, President Iván Duque announced Colombia’s withdrawal from the Union of South American Nations (UNASUR) after criticising its accommodative stance on Venezuela and due to disagreements over which country should provide the regional bloc’s next president. The move to leave UNASUR is expected to be completed within the next six months.
The economy is expected to accelerate this year on higher oil prices, which should boost investment in the extractive sector. Planned infrastructure projects and rising investor confidence stemming from reduced political uncertainty should help lift growth. Duque’s plans to slash corporate taxes, however, could present challenges in meeting the fiscal target, unless they are offset with new sources of revenue. FocusEconomics panelists expect GDP to grow 2.7% in 2018, which is up 0.1 percentage points from last month’s forecast, and 3.1% in 2019.
MONETARY SECTOR | Regional inflation rises in July
A comprehensive estimate, without considering the current period of hyperinflation in Venezuela, revealed that inflation rose in July. FocusEconomics estimates that inflation in Latin America (excluding Venezuela) came in at 6.2%, above June’s 5.9%. Pass-through from a weak peso caused inflation to spike in Argentina, while Brazil also saw higher price pressures due to electricity price hikes, a weak real, and higher gas prices. In addition, higher energy prices caused inflation to build in the remaining economies, including in Mexico. However, a preliminary estimate for August revealed that inflation was broadly stable at 6.1%.
Following the peso’s downward spiral, policymakers in Argentina raised interest rates to an all-time high in August to stem the currency’s slide. Elsewhere in the region, rising interest rates in the U.S. have also eroded space for monetary easing as central bankers try to prevent capital flight. Policymakers in Peru left interest rates unchanged in August after they had paused the easing cycle in March.
Regional inflation excluding Venezuela is seen rising by the end of the year, coming in at 7.1%. The forecast was revised up 0.6 percentage points from last month’s projection, largely due to upward revisions to Argentina and Brazil’s inflation projections because of weaker currencies. Chile, Mexico and Uruguay’s inflation forecasts were also revised up. In 2019, inflation is seen ending the year at 5.3%. Venezuela is experiencing an episode of hyperinflation and is not included in the aggregate.

domain: geography


There’s no doubt that Batman: Arkham Knight was a phenomenal game, and showed what British developer Rocksteady could do with the Batman license. It was a truly terrific superhero game which even managed to top the superb Batman: Arkham City, but it is now more than four years old.
And Batman fans have been eagerly awaiting another video game featuring the Caped Crusader for a long time now…
Sadly, Rocksteady almost certainly won’t be making more Batman games, even if the British outfit is now hard at work on another superhero game which is yet to see the light (early rumours said Superman might be the protagonist, but Rocksteady has since dismissed these).
So, does this mean Batman fans won’t be getting a new Batman game anytime soon?
Not exactly, as rumours have been floating around that a new Batman game could be announced soon as was recently reported on Push Square.
A teaser on Twitter earlier this year from WB Games Montreal got many people’s hopes up, and now rumours suggest this new Batman game could be announced at the Game Awards on December 12 this year. This is apparently confirmed by a YouTuber who’s in contact with a leaker who also provided details on an earlier Batman game (which got cancelled) as covered on the Daily Star.
An announcement this big won’t be out of place at the Game Awards of course, as several high profile games such as the next Dragon Age have been announced at the awards show in the past.
Capture the Knight / Cape sur la nuit pic.twitter.com/yMFXMd4djU
— WB Games Montréal (@WBGamesMTL) September 23, 2019
This next Batman game could be titled Batman: Arkham Legacy, and will be made by the same people behind Batman: Arkham Origins. The 2013 game isn’t the best entry in the Arkham series by far (arguably it’s the worst), so it would be a great thing if WB Games Montreal can produce a better game this time, one which will hopefully rival the Arkham games by Rocksteady.
Batman: Arkham Legacy’s plot will supposedly focus on the Court of Owls – the secret society and criminal organization of Gotham City which feature in the comic books – and which have the nasty habit of kidnaping child performers to turn them into assassins, meaning this new Batman game could have an interesting story at its core for sure.
There’s not much longer to wait in order to find out more about this new Batman game if the rumours are proven correct, so hopefully we’ll have great news for fans of the Caped Crusader when the Game Awards kick off on December 12.


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OCEAN CITY- The newest addition to the summer fishing tournament schedule in the resort area- the 1st Annual Wahoo Rodeo and Flounder Round-up- got underway this morning with the first of three official fishing days.
Billed as “truly a tournament for everyone,” the first-ever Wahoo Rodeo and Flounder Round-up debuts this weekend with an opportunity for offshore anglers and bay fishermen to compete in a fun event with cash and prizes in several categories including, as the name implies, the top three largest wahoo and flounder caught during the event. Other species targeted include tuna and dolphin.
The event got started yesterday with registration and a captain’s meeting at host Sunset Marina in West Ocean City. The action in the water got underway this morning with the first of three fishing days. Captains must choose to fish two of the three days, which include Friday, Saturday, and Sunday.
Offshore, tournament participants will target wahoo, dolphin and tuna with a boundary limit of 100 nautical miles from the sea buoy. Participating boats may leave from the Ocean City or Indian River Inlets, but qualifying fish must be weighed at the host Sunset Marina. In the offshore segment of the tournament, lines can go in at 8 a.m. and must be out at 3:30 p.m.
The inshore segment of the event will target flounder and prizes will be awarded for the top three largest fish. The boundaries for the Flounder Round-up include anywhere in between the Route 90 bridge and the Verazzano Bridge and one nautical mile off the coast of the resort. Lines can go in at 6:30 a.m. and must be out by 3:30 p.m.
Prizes will be awarded to the top three largest fish in each division with various added entry levels available to enhance the payouts. The minimum weight for wahoo and tuna is 30 pounds, while the minimum qualifying weight for a dolphin is 20 pounds. The existing size limits for flounder will apply and IGFA rules will apply for all activity offshore. For example, live bait, chunking and wire line are permitted.
In addition, there will be a Top Lady Angler prize for the largest wahoo or flounder caught by a female age 16 or older and a Top Junior Angler prize for the top wahoo or flounder caught by a youth age 15 or younger. In both cases, the angler must accompany the winning fish to the scales.
Sunset Marina will host the event with daily weigh-ins from 4-7 p.m. on Friday and Saturday and from 4-6:30 p.m. on Sunday. A banquet and awards ceremony will be held at the conclusion of the event on Sunday at the Ocean City Marlin Club.


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nutcracker round gift tag
Regular price $45.00
elevate your gift tags with gorgeous 1.25" hand-dyed raw silk ribbon.
choose up to THREE colors to be divided evenly across your set
each ribbon is dyed using labor-intensive methods and crafted with the finest silk thread. thanks to an old-world weaving technique, each ribbon has a subtle textured look and cross-woven threads while actually being smooth and soft. this ribbon is delicate and beautiful. this fine silk possesses true heirloom quality. choose up to 3 ribbon colors.
- please provide the name you would like printed
- if you you selected 'yes' to raw silk ribbon, please specify the colors
sold in sets of 24

domain: artistic


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Originally Posted by CloakNNNdagger
Today Luck has had an occasional flash. But, he is definitely showing lots of rookie mistakes.......delay of game, several questionable decisions on throws
and backing up into a safety. And it seems that he will be getting no help this year from the Colts running game.........which appears to be nonexistent.
Unless the box score is wrong that was Harnish and not Luck that got the safety.


Dognerdz Friends Tee - Unisex
Adorable group of dognerdz dogs on a short sleeved shirt including Boxer, German Shepherd, Pug, Chihuahua, Husky, and Poodle!
Original dognerdz design.
dognerdz icon on left sleeve in white
Unisex sizing - women may want to size down
Navy is 100% combed and ring-spun cotton. Heather colors are 52% combed and ring-spun cotton/48% polyester. Black Heather is 90% combed and ring-spun cotton/10% polyester.


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Surface plasmon resonance (SPR) biosensors have been recognized as a useful tool and widely used for real-time dynamic analysis of molecular binding affinity because of its high sensitivity to the change of the refractive index of tested objects. The conventional methods in molecular biology to evaluate cell differentiation require cell lysis or fixation, which make investigation in live cells difficult. In addition, a certain amount of cells are needed in order to obtain adequate protein or messenger ribonucleic acid for various assays. To overcome this limitation, we developed a unique SPR-based biosensing apparatus for real-time detection of cell differentiation in live cells according to the differences of optical properties of the cell surface caused by specific antigen-antibody binding. In this study, we reported the application of this SPR-based system to evaluate the osteogenic differentiation of mesenchymal stem cells (MSCs). OB-cadherin expression, which is up-regulated during osteogenic differentiation, was targeted under our SPR system by conjugating antibodies against OB-cadherin on the surface of the object. A linear relationship between the duration of osteogenic induction and the difference in refractive angle shift with very high correlation coefficient was observed. To sum up, the SPR system and the protocol reported in this study can rapidly and accurately define osteogenic maturation of MSCs in a live cell and label-free manner with no need of cell breakage. This SPR biosensor will facilitate future advances in a vast array of fields in biomedical research and medical diagnosis.
Citation: Kuo Y-C, Ho JH, Yen T-J, Chen H-F, Lee OK-S (2011) Development of a Surface Plasmon Resonance Biosensor for Real-Time Detection of Osteogenic Differentiation in Live Mesenchymal Stem Cells. PLoS ONE6(7): e22382. https://doi.org/10.1371/journal.pone.0022382
Editor: Cesario V. Borlongan, University of South Florida, United States of America
Received: May 12, 2011; Accepted: June 20, 2011; Published: July 27, 2011
Copyright: © 2011 Kuo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported in part by the UST-UCSD International Center of Excellence in Advanced Bioengineering, sponsored by the Taiwan National Science Council I-RiCE Program under Grant Number: NSC-99-2911-I-009-101. The authors also acknowledge financial support from the Taipei Veterans General Hospital (VGH100E1-010, VGH100C-056, VN100-05 and VGH100D-003-2) and the National Science Council, Taiwan (NSC99-2120-M-010-001, NSC99-2627-B-010-003, NSC99-3111-B-010-002, NSC98-2314-B-010-001-MY3, NSC 99-2911-I-010-501, NSC 99-3114-B-002-005, and NSC 99-2112-M-010-001-MY3), as well as Wan Fang Hospital (100swf03). This study was also supported by a grant from the Ministry of Education, Aim for the Top University Plan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Dramatic progress in the biological understanding and the potential clinical use of mesenchymal stem cells (MSCs) has been made in recent years. MSCs have been initially identified in bone marrow stroma as non-hematopoietic stem cells which are capable of differentiation into tissues of mesodermal origin, such as osteoblasts, adipocytes, chondrocytes, tenocytes, and hepatocytes , , , , , . Due to their multi-lineage differentiation potentials, many pre-clinical studies with tissue engineering approaches are currently under investigation , , . Previously, we have established a platform to isolate and to expand single cell-derived, clonally expanded MSCs from human bone marrow and umbilical cord blood through negative immune-selection and limiting dilution , . These single cell-derived hMSCs are highly homogenous in morphology; and possess a high capacity of in vitro expansion and multi-lineage differentiation.
Osteoblasts, which are progenies of MSCs, are bone-forming cells and play an important role in the homeostasis of the skeletal system , , . Current strategies for the differentiation of stem cells commonly include induction with mechanical or chemical stimuli. To evaluate the maturation of osteogenic differentiation of hMSCs during these processes, histochemical and molecular biological methods such as alkaline phosphatase (ALK-p) staining, von Kossa staining, Western blot, and reverse transcription polymerase chain reaction (RT-PCR), are commonly used , , . However, all these traditional methods are time-consuming with tedious process and can only provide semi-quantitative or non-quantitative data except for the real-time RT-PCR. Moreover, the conventional methods to detect the extent of osteogenic differentiation require cell lyses or fixation, which causes cell death and makes continuous analysis on the same cell impossible.
Surface plasmon resonance (SPR) biosensors dedicated to biomolecular dynamics and recently to cell analysis have generated tremendous interest in developing new tools for both diagnostic and research purposes. This technique is a surface-sensitive method of increasing interest for bio-analysis as it allows label-free and real-time analysis of biomolecule interactions on functionalized surfaces , , , , , . The primary goals for the development of this technique is to establish a method with rapid live cell analysis, high throughput, and small sample volumes . For this purpose, selection of a proper surface marker for osteogenesis is imperative. The cell transmembrane protein, OB-cadherin, firstly cloned in 1994 , , is known to selectively express in osteoblastic cell lines, precursor cell lines of osteoblast, and primary osteoblastic cells . The purpose of this study is to investigate whether the SPR technique can be used as a live cell sensor to accurately define the different stages of osteogenic maturation in live cells by detecting the expression of OB-cadherin on cell surfaces.
2.1 Culture maintenance and expansion
For studies involving human tissues we obtained Institutional Review Board approval of Taipei Veterans General Hospital on March 24th, 2010 and written patient informed consent. Bone marrow was collected from healthy young donors during fracture surgery after Institutional Review Board approval and informed consent. Mononuclear cells from the bone marrow were isolated and MSCs were purified with negative immuno-selection and limiting dilution as previously described . SaOS2  is an OB-cadherin expressing cell line and is used as a positive control. Hep3B is a human hepatoma cell line  and served as an OB-cadherin non-expression control. Expansion medium for MSCs consists of a commercially available medium (MesenPro, Gibco, Grand Island, NY) supplemented with 100 U penicillin, 1000 U streptomycin, and 2 mM L-glutamine (Gibco). Expansion medium for SaOS2 and Hep3B consists of Iscove's modified Dulbecco medium (IMDM; Gibco, Grand Island, NY) and 10% fetal bovine serum (FBS; Hyclone, Logan, UT) supplemented with 100 U penicillin, 1000 U streptomycin, and 2 mM L-glutamine (Gibco).
2.2 Osteogenic differentiation
To induce osteogenic differentiation, MSCs were treated with osteogenic medium for 15 days with medium changes every 3 to 4 days. Osteogenesis was analyzed every 3 days. Osteogenic medium consists of IMDM supplemented with 0.1 µM dexamethasone (Sigma-Aldrich, St Louis, MO), 10 mM β-glycerol phosphate (Sigma-Aldrich), and 0.2 mM ascorbic acid (AsA; Sigma-Aldrich).
2.3 Cytochemical staining of osteogenic differentiation
To analyze osteogenesis, cells were rinsed twice with PBS, fixed with 3.7% formaldehyde for 20 minutes, and washed with distilled water. Alkaline phosphatase (Alk-P) histochemical stain was performed using the nitroblue/BCIP solution (Roche Molecular Biochemicals, location?) based on the manufacturer's instructions. Mineralization matrix was analyzed with von Kossa staining using 1% silver nitrate (Sigma-Aldrich) under UV light for 45 minutes, followed by 3% sodium thiosulfate (Sigma-Aldrich) for 5 minutes, and then counterstained with van Gieson (Sigma-Aldrich) for 5 minutes.
2.4 Antibody coating of Au chip
Au chip was immersed in 1 mM 11-MUA in 75% alcohol for 12 hr, followed by immersion in 2 mM∶5 mM EDC/NHS for one hour. The chip was then coated with 0.005 ug/ml protein G in PBS for one hour, and bound with 0.05 ug/ml mouse IgG anti-human OB cadherin (Abnova) or mouse IgG (sigma) in PBS for one hour, and finally blocked in 1 mM BSA.
2.5 Surface plasmon resonance system
The experimental setups for the evaluation of osteogenic differentiation of MSCs are schematically illustrated in Fig. 1. The setup combines the platforms of the biosensor for the excitation of surface plasmon polaritons (Fig. 1A) and a fluidic cell chamber (Fig. 1B). As illustrated in Fig. 1A, a Helium–Neon laser of wavelength 632.8 nm was used as the light source to excite surface plasmon polaritons. The extinction ratio of beam polarization was enhanced to 1000∶1 through the aid of a polarizer in front of the laser. This polarizer was oriented to let only the p-wave pass. A beam splitter (BS) was used to separate the laser beam into two beams. One p-wave was reflected to a detector for normalization of the laser power instability and the other was transmitted to excite surface plasmon polaritons. Since the dispersion characteristic of surface plasmon polaritons forbids a p-wave in free space couple to a surface plasmon wave due to the smaller spatial phase of the wave in free space. A prism made of SF11 adds an extra spatial phase on the laser beam through its high refractive index, n = 1.78, to match the dispersion relation of the surface plasmon polaritons. For a practical reason, an Au thin layer with thickness 47.5 nm was coated on a glass slide made of SF11 as the platform of the surface plasmon polaritons. Matching oil was applied between the prism and the glass slide to avoid multi-reflection occurring between them. The energy coupling between the laser beam in a free space and the surface plasmon polaritons bound on the Au-dielectric interface was measured through the reflection of the laser beam from the Au chip. This reflection served as the indicator of the coupling or excitation efficiency. This excitation efficiency was optimized when the incident angle of the laser beam is properly adjusted. This optimized incident angle, known as the resonance angle, is determined by the refractive index of any material contacting the Au chip and the wavelength of the excitation light beam as well. In this system, the incident angle of the laser beam was controlled by a motorized rotation stage through a controller, and a silicon photodetector was controlled accordingly by another motorized rotation stage to measure the power of the reflected laser beam. These two rotation stages (Sigma Koki, SGSP-120YAW) controlled by the two-axis controller (Sigma Koki, SHOT-202) have the angular resolution of 2.5×10−3 degrees. The intensity of the reflected beam as a function of the incident angle was recorded by a computer via 16-bit A/D converter (Adventech PCI-1716). A cell chamber equipped with a fluidic channel was constructed on the SPR platform to provide the living cells for testing and to flow deionized (DI) water for stabilizing and for washing unbound cells. The temperature of the cell chamber was stabilized at 37°C by a TE cooler placed on the top of the chamber. The temperature fluctuation is less than 0.1°C through a temperature controller.
(A) Schematic illustration of the SPR device: The He-Ne laser penetrated through a polarizer and a beam splitter, which split the beam 50/50. One was detected by a photodiode and the other one was coupled by a prism to generate Surface Plasmon Wave on the Au chip in which the angle shift was detected by a photodiode. (B) The OB-cadherin expressing cells flowing into the chamber were captured by the Au chip pre-coated with OB-cadherin antibodies, which changed the angle of the reflected laser beam. (C) A typical graphical data from SPR measurements.
2.6 Experimental procedures
As depicted in Fig. 1B and Fig. 1C, undifferentiated MSCs, MSCs under osteogenic induction for different days, Hep3B and SaOS2 cells were individually mixed with PBS and injected into cell chambers with Au chips and subject to three different surface treatments: one without antibody coating, one with mouse IgG antibody coating (non-specific binding control), and one with pre-coated with OB-cadherin antibody. The flow rate and cell number was controlled at 1000 cells/min. The angle shifts of captured cells on Au chips subject to the three different surface treatments were measured and recorded by the SPR system operating in the angular interrogation mode as previously described. After the angle increased to the level of saturation, flushing with PBS was performed to wash out the cells until the angle shift returned to the initial level. The subsequent population of cells was then injected on the Au chip for further measurements.
2.7 Quantitative real-time PCR analysis
Total RNA was isolated from 3×105 cells using Trizol (Invitrogen) or a commercially available kit (RNA easy minikit, Qiagen, Courtaboeuf, France). We reverse transcribed the messenger RNA to complementary DNA using reagents (Genemark Technology, Taiwan) according to the manufacturer's instructions. Quantitative real-time PCR analysis was performed and cDNA was amplified at 95°C for 60 seconds, 56°C for 45 seconds, and 72°C for 60 seconds for 40 cycles, after initial denaturation at 95°C for 5 minutes using an ABI Step One Plus Real Time PCR System. The specific primer sequences are 5′-AAACAGCCTGGCTCAACATC-3′ (forward) and 5′-CTTCCTGATGCCGATTGTG-3′ (reverse) for OB-cadherin; 5′-AGCCACATCGCTCAGACAC-3′ (forward) and 5′-GCCCAATACGACCAAATCC-3′ (reverse) for GAPDH.
2. 8 Western blot analysis
106 cells were collected and resuspended in 200 µl Extraction Buffer I and 1 µl protease inhibitors cocktail (Subcellular proteome extraction kit, Merck). Cell lysate was incubated on ice for 10 min. with gentle agitation and then centrifuged at 1000 g at 4°C for 10 min. The supernatant was collected, and remixed in 200 µl Extraction Buffer II and 1 µl protease inhibitors cocktail (Subcellular proteome extraction kit, Merck KGaA). Cell lysate was incubated on ice for 30 min. with gentle agitation and then centrifuged at 6000 g at 4°C for 10 min. The supernatant was collected and protein concentration was measured by Bradford assay (Bio-Rad, Hercules, CA). 10 µg/well of protein was separated on 10% sodium dodecyl sulfate/polyacrylamide gel electrophoresis and blotted onto a polyvinylidene difluoride membrane (GE Healthcare Bio-Sciences, Piscataway, NJ). Nonspecific bindings were blocked by 5% skim milk in TBST buffer (50 mmol/L Tris-HCl, 150 mmol/L NaCl, 0.1% Tween 20, pH 7.4). The membrane was sequentially hybridized with the primary anti-OB cadherin antibody (1∶2000, Abcam) and with horseradish peroxidase–conjugated secondary antibody (1∶6000, GE Healthcare Bio-Sciences) at room temperature for 1 hour. Washes were performed between incubations. Protein intensity was resolved with enhanced chemiluminescence reagent (PerkinElmer, Wellesley, MA).
The mRNA and protein expression level of OB-cadherin increased following the maturation of MSCs during osteoblast differentiation (Fig. 2A and Fig. 2B). The OB-cadherin protein expression level was measured by a semi-quantitative assay in which the relative amount of immunoreactive bands on Western blot film was quantified as arbitrary units using a computerized software program (LabWorks 4.6; UVP Inc., Cambridge, UK) and was normalized to the intensity of SaOS2 (Fig. 2C). The result demonstrated an increase in OB-cadherin expression level as the induction period increased. In addition, successful induction of osteogenic differentiation was confirmed by alkaline phosphatase (AP) and von Kossa staining (Fig. 2D). Since the expression of the membrane molecule OB-cadherin is up-regulated during maturation of osteoblasts, we therefore used it as a marker to analyze the extent of differentiation by quantifying it under SPR system. In order to eliminate non-specific binding of cells to Au chip and false positives, SaOS2 and Hep3B cell lines were used as positive and negative controls for the expression of Ob-cadherin respectively. Furthermore, the Au chip without antibody pre-coating or with mouse IgG antibody pre-coating were used as control groups to eliminate non-specific binding of cells onto Au chip and IgG antibodies. DI water was first injected into the cell chamber for stabilization, followed by PBS as a baseline control. After the injection of PBS, cells were injected for measurements. The true angle shift of each cell line was evaluated by subtracting the resonance angle in the corresponding test baseline from the SPR resonance angle of bound and unbound cells. For the non-coating control groups, SaOS2, Hep3B, and MSCs were injected into the cell chamber without antibody pre-coated on the Au surface. As expected, all the tested cell lines showed no significant difference on bare Au chips because there is no antibody layer to perform specific binding (Fig. 3A). For the antibody coating control groups, the anti-OB cadherin and mouse IgG antibodies were first immobilized on the sensor chips and bathed in a concentration of 103 cells/ml PBS by the self-assembly monolayer (SAM) coating method. The amount of resonance angle shift after injecting the SaOS2, Hep3B, MSCs to the cell chamber with mouse IgG also showed no significant difference because there is no specific cell-antibody binding (Fig. 3B).The values shown in Fig. 3B were used as the non-specific binding background of each cell line and were denoted as respectively for later analysis. On the contrary, in the group with OB-cadherin antibody coating, the angle shift of SaOS2 cells were distinguished from the Hep3B and the MSCs at day zero. This result demonstrated that SaOS2 has significantly higher OB-cadherin expression on cell surfaces, but none on Hep3B and on MSCs without osteogenic induction.
(A) Real-time PCR of OB-cadherin expression. (B) Western blot of OB-cadherin expression during osteogenic induction of MSCs. SaOS2 served as positive control. (C) The OB-cadherin expression level intensity analyzed by semi-quantitative method and was normalized to the intensity of SaOS2. (D) Alkaline phosphatase (AP) staining and von kossa (VK) staining.
(A) Test control of antibody non-coating system and injected with SaOS2, Hep3B and MSCs onto Au chip. (B) Test control of antibody coating system by injecting SaOS2, Hep3B and MSCs onto mouse IgG pre-coated Au chip. (C) The verification and stability test of OB-cadherin antibody-antigen binding by injecting SaOS2, Hep3B and MSCs.
Since MSCs that express more OB-cadherin on the cell surface had a better opportunity to be captured by the coated antibodies; this results in an increment of refractive index in the detection region, indicated by the angle shift. Therefore, the resonance angle with a larger value would be obtained. The measurement was first conducted by the injection of the MSCs into the cell chamber. After the binding of MSCs reached the saturation status, PBS was used to wash out the MSCs followed by the injection of SaOS2 cells to measure the number of the active binding sites expressed in the SPR angle shift. A typical curve of SPR angle shift over the entire experimental procedure was demonstrated in Fig. 1C. In order to investigate the relative expression of OB-cadherin compared to the OB-cadherin positive SaOS2 cells, we used the angle shift obtained from SPR measurements to calculate the angle difference between the cells. As expression levels become similar, the angle difference will approach zero. Using the resonance angle before the injection of the MSCs as a reference value, denoted by , to subtract the value of the non-specific binding, , the amount of OB-cadherin expression on the membranes of the MSCs is then proportional to the shift of the resonance angles as . During each test, the different Au chip may have different number of active binding sites formed by anti-OB-cadherin, this number of active binding sites on the Au chip was measured by the angle shift starting from the injection of SaOS2 and ending at the saturation of the cell binding. The calculation can be formulated as . In the experiment, the relative value of the SPR angle shifts between MSCs and SaOS2 cells, indicated as OB-cadherin expression level , was normalized for data analysis. This normalization eliminated the variation of anti-OB-cadherin coating for each measurement. When the duration of osteogenic induction increased, the experimental value of angle difference () from MSCs subjected to different culture durations gradually approached to zero after 15-days (Fig. 4A).The interval of the MSC culture time for this measurement is three days until OB-cadherin levels on MSCs approached to the highest expression level and bound to anti-OB-cadherin antibodies with the same binding strength as SaOS2. This result suggested that both cell types are similar in surface OB-cadherin expression on the 15th day. Furthermore, when the angle difference was normalized to and denoted as , this normalization eliminated the variation of anti-OB-cadherin coating for each measurement. This quantity represents the complement of OB-cadherin expression. Therefore, the amount of OB-cadherin can be expressed as . The experimental value of OB-cadherin expression level from MSCs subject to different culture durations was calculated. As expected, this angle difference approached to one after 15 days when the duration of osteogenic induction increased (Fig. 4B). The analysis showed that the expression level of OB-cadherin increased linearly with the increase of the culture time (days). By using this expression level curve as a standard, we could easily predict the maturation level of unknown differentiated live cells.
In this study, we developed a real-time, cell-preserving, and quantitative measurement method based on the surface plasmon resonance bio-sensing technique to characterize the maturation of osteoblasts by selective binding of a cell surface molecule, OB-cadherin, which is specifically and highly expressed in differentiating osteoblasts. With pre-coated antibodies on an Au chip, alteration of SPR angle shift could be accurately measured and analyzed. From the data analysis, it was found that the OB-cadherin expression level was increased as the culture time increased. The expression level obtained in the SPR biosensing method correlated with the result obtained by the conventional method of Western blot, but with much higher sensitivity at nanogram levels . This new development offers a live cell tool to accurately define the different stages of osteogenic differentiation in live cells. Moreover, although the measurement was performed on osteoblast maturation, similar approaches can be applied in future studies to quantify the expression of OB-cadherin in tumor cells to predict their invasiveness and to also use other surface markers to monitor differentiation in other stem cell types .
In comparison with the results shown in Fig. 2C and Fig. 4B, the OB-cadherin expression level obtained by the Western blot assay in the early culture period did not match that obtained by the SPR biosensor, especially before the 9th day of osteogenic differentiation. As shown in Fig. 2B, OB-cadherin expression was undetectable before day 6, with relatively weak signals until day 12. It is evident that Western blot detection lacks the ability to account for the vast range difference in OB-cadherin expression. For example, OB-cadherin expression at day 6 can be enhanced by prolonged exposure to chemiluminescent reagents. However, this would also saturate the reaction of HRP and chemiluminescent reagents of the day 15 group, resulting in inaccurate quantification. This explains why the relative quantitative level of OB-cadherin obtained by Western blot did not coincide with the result obtained by the SPR technique, but only coincided qualitatively in trend of expression. In addition, the most valuable advantage of this technique is its detection sensitivity whereby differences in OB-cadherin expression could be quantitatively distinguished by SPR technique during early differentiation stages between day 0 and day 6 (Fig. 4A), and not by Western blot (Fig. 2C). This also suggests that SPR is more accurate than the conventional Western blot method. Most importantly, live cells can be analyzed, which is not possible for the conventional Western blot method.
In summary, this SPR protocol makes possible the quantitative evaluation of different cell surface molecules on live cells in a time-saving and cell-saving manner for future cell biology research.
Conceived and designed the experiments: JH HFC OK-SL. Performed the experiments: YCK. Analyzed the data: Y-CK JH T-JY OK-SL. Contributed reagents/materials/analysis tools: JH T-JY HFC OK-SL. Wrote the paper: Y-CK HFC OK-SL.
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- 30. Lin SH, Huang CF, Lira C, Chu K, Bilen MA, et al. (2010) Cadherin-11 Increases Migration and Invasion of Prostate Cancer Cells and Enhances their Interaction with Osteoblasts. Cancer Research 70: 4580–4589.SH LinCF HuangC. LiraK. ChuMA Bilen2010Cadherin-11 Increases Migration and Invasion of Prostate Cancer Cells and Enhances their Interaction with Osteoblasts.Cancer Research7045804589

domain: medical


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Analysis and insight for the UK fresh produce business.
MorePeople CEO Andrew Fitzmaurice discusses the future role of AI and automation in recruitment, and argues there is still much value to be gained from the human touch
As Eurofruit’s Fresh Focus South Africa special reveals, disruption in South African ports has left the country’s fresh produce exporters with a mountain to climb
NFU Horticulture & Potatoes Board chair Martin Emmett calls for urgent government action to help the UK horticulture sector continue to make a positive contribution to the British economy
Of all the perishable cargoes affected by the Panama Canal drought, the Red Sea crisis, and infrastructure issues in South Africa, table grapes have been worst hit. And now, market prices may be about to fall
Mike Parr, director of logistics firm PML Seafrigo, lays bare the potentially dire consequences of Defra’s controversial decision to reintroduce physical checks on imports of fresh produce from the EU
Compliance consultant Jim Jefcoate sets out the tangible differences between the two approaches with a view to helping fresh produce companies improve their auditing operations
Roberto Zanichelli, business development and marketing director at Ilip, offers his insights into the changing dynamics of the fresh produce packaging business
Richard Clark of market analyst MrQual Research delves into data it has gathered on the factors that are important to UK shoppers when buying fruit and vegetables
Any company that wants to secure its future must integrate sustainability into its business plan. But that’s easier said than done, says Richard Bonn, co-founder of Aethr Associates
The Fresh Produce Journal is the UK's leading source of fruit and vegetable industry news and analysis.
Subscribe and get 10 issues per year. Plus newsletters and full digital access.
Looking ahead to the next 12 months, John Giles of Promar International predicts further change in the fresh produce industry due to digitalisation, inflation, trade friction and climate change
The Italian government has backed Melinda’s unique plan to remove truck journeys between its Predaia packhouse and its mountain storage centre
Tim O’Malley, group MD of Nationwide Produce, reflects on the weather challenges facing growers of British vegetables as production is driven abroad and the sector lacks support from government
Reefer Trends editor Richard Bright explores whether abandoning the import tariff on Latin American bananas would amount to a betrayal of producers in West Africa, as the Afruibana trade body contests
Compliance consultant Jim Jefcoate makes the case for a more holistic and less burdensome approach to fresh produce certification based on ‘quality culture’

domain: economics


text:
Jammu and Kashmir Hearing and speech impaired Bashir Ahmed leads by example in painting and cricket jkmun snm
Until now, he has created countless amazing and incredible paintings. These young people adhere to the demands of fine art.Bashir Ahmed wants to improve his skills and reach heights instead of being a victim of an inferiority complex. But until now, no one has paid attention to its potential, nor has it been given a platform to advance. Basheer works as an ordinary employee in the Gulmarg Gondola located in the tourist destination of Gulmarg.
Bashir Ahmed’s family members say he was posted to Gulmarg so that he could attract tourists from all over the world by painting here. They can also make a living, but the irony is that later on, the relevant department or government does not pay any attention to their art and they have to work as a casual employee in the gondola.
Whenever I go to the toilet, someone follows me, this cricketer said about the security situation in Pakistan
Shoaib Malik also narrated Shahid Afridi’s big revelation about the abuse of a Faisalabad boy in 2005.
Bashir completed his education up to 9th grade. Bashir is married with two children. Hazak, son of Bashir, says that everyone considers the house a sign of his father. He appeals to provide them with work somewhere. Family members say they have been told many times by the government that samples of Bashir’s painting will be kept in the exhibition halls, but this could not be done so far. Bashir has reserved a separate room in his house for this painting. painting, they have provided everything but paints and pigments.
He has taken countless pictures till date. The perfection of his artistry and painting is that he made a perfect picture of all the high officials or officials who came to Gulmarg. Among them many personalities are gifted Bashir Ahmed also plays cricket. He has also formed a team of speech and hearing impaired youth. Of which he himself is the captain. Bashir Ahmad is a master of every art. He wants the government to play an important role in providing rights to able-bodied and physically disabled people by filling the gaps. At the same time, Bashir’s art should also be promoted.
First read news in Urdu on News18Urdu, breaking news in Urdu. Today’s latest news, live news update, read the most trusted news in Urdu on news18 urdu.com, learn all the details related to your state, country and abroad and especially Middle East, entertainment, sports, business, health, education and employment. Follow News18 in Urdu on Twitter, Facebook, Instagram, YouTube, DailyHunt, ShareChat and Quoap.


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Train to Pakistan A Novel by KHUSHWANT SINGH
Read by Kirti Rohilla
KHUSHWANT SINGH is one of India’s most widely read writers and journalists, admired and lambasted in equal measure because of his often irreverent and controversial views. Even his staunchest critics, however, agree that he is never less than eminently readable.
Born in 1915, khushwant Singh made his literary reputation with his very first novel, Train to Pakistan, which was followed by several volumes of short stories and books on contemporary affairs.
His career as top flight journalist began in 1969 as editor of the illustrated weekly of India, followed by stints as editor, new Delhi magazine and the Hindustan Times. Now as a syndicated columnist, his readership is larger than ever before. Arthur lall, Who wrote the introduction, is the author of the emergence of Modern India, published by Columbia University press.
To Download or Play click:- Train to Pakistan A Novel by KHUSHWANT SINGH

domain: literature


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“Everything’s back to normal; but we both privately know that what’s normal has changed, and changed for ever.” (Michael Frayn)
Okay, so firstly, I’m not in Japan yet. It’s still June and I still haven’t got my Certificate of Eligibility, let alone my final year marks from UEA. But as I’ve said before, I want to start building this blog and filling it with content that would be here anyway in the future (my life in Japan is going to be through my eyes – thus, my hobbies and my life will frame the narrative along the way. I’m not interested in writing a factual wordsplosion on The Natural Beauty of Japan or anything of the sort)
So I’m excited to begin with discussing a few things that I’ve been reading recently. In my second year of uni, I was so focused on my studies and my fear of failure that I basically stopped having hobbies other than studying 24 hours a day. So while I’ve been, and will be, at home, from 6th June – 10th July, I’ve been doing some crazy catching up. Other than the three books I’ll be discussing, I’ve been absorbing gems from Carrie Fisher’s The Princess Diarist all the way to Meik Wiking’s The Little Book of Hygge. Doing everything I essentially forbade myself from doing is, to be honest, something that has made me the most comfortable and happy I’ve been in a while. That’s important because I’ll be confronting a certain something for the first time in 9 months when I leave England in July, and I think I’m reaching the stage in which I’ll feel prepared for it.
But in any case; onto the three lovely books.
This is a book I’ve been sleeping on, and a massive reminder of how much I love mystery novels. I first began reading it in the Summer of 2016, but clearly I got distracted by something or another and left a bookmark half way through – when I reopened it a few days ago, I’d completely forgotten what the book was even about. It takes place in the time period after The Final Problem, but before The Return of Sherlock Holmes, and is told from the perspective of American detective Frederick Chase who comes to London following the events of the Reichenbach Falls.
If you know nothing about the Sherlock Holmes series, don’t bother, but if you do, Moriarty will definitely not disappoint you. I’ve never once finished one of Conan Doyle’s stories to the very end because I find 19th century literature incredibly heavy to read, but I absolutely love BBC’s Sherlock
minus the 4th Series and that was enough for me to enjoy Moriarty. I’m not embarrassed to admit that I even believed Athelney Jones was a character Horowitz created for this novel. That’s how little you need to know of Conan Doyle’s canon.
This book has one of the biggest and most satisfying reveals I’ve read in a mystery novel. You’ll most definitely find yourself rereading the entire thing, asking yourself how you could’ve possibly missed the clues.
I’m not generally a fan of science fiction – the only genres of fiction I really enjoy are mystery, thriller, comedy. The fourth genre I enjoy is social commentary, which I suppose only exists in fiction as a sub-genre of another (Harper Lee’s To Kill a Mockingbird and John Green’s Looking for Alaska stand side by side as my favourite novels and I’d classify them both as Bildungsroman/Social-Commentary, albeit on opposite sides of the spectrum).
Cat’s Cradle switches that formula around. It sits on a Science Fiction base, but truthfully it’s more of a commentary on the potential disastrous consequences of humans having the power to create and destroy on a massive scale, using America’s nuclear bombings of Nagasaki and Hiroshima as a backdrop to discuss Vonnegut’s creation of Ice-Nine, a substance that turns room-temperature liquid into ice. (Put that into the ocean.) Vonnegut manages to perfectly balance the above with commentary on Religion and the purpose of life.
It’s incredibly easy to read, written in the style of sub-chapters, with an ending that can only be described as simple yet powerful. I just sat there for maybe 10 minutes after finishing the final page, letting myself just… absorb everything. An incredibly haunting read, more so because it is potentially prophetic.
Steven D. Levitt & Stephen J. Dubner
Pretty sure I was asked to read this for AS Economics. Pretty sure not remembering whether or not I was asked to read it has some kind of a correlation with why I did badly at AS Economics. I suppose, at the end of the day, I’ve gotten around to reading it and that’s good, never mind that it’s 4 years on.
To tell you the truth I’ve only just begun reading Freakonomics, but I know it’ll have me in a trance right until I’ve finished it and there are other things I’d like to be doing. Levitt and Dubner (to quote the blurb) “[use] information about the world around us to get to the heart of what’s really happening under the surface of everyday life.” It’s this kind of stuff that really, really interests me, so I know for a fact that I’ll be loving this book start to end. I touched upon it, but I much prefer non-fiction to fiction; the big two are autobiographies and books that explain exactly why certain things occur, with Freakonomics fitting neatly under the latter.
So what now?
I carry on reading, and I carry on cooking, and I carry on watching, and I carry on living the status quo right up until I leave for Japan and everything changes. Perhaps I will update a few more times before I go, while we’re in the “before” phase. Maybe a post about a recipe I utilised? I’ve had that on my mind for a while but figuring out how best to execute it is difficult. I have the determination, but just maybe not the inspiration.
This is going to be a crazy experience. No more running away.


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Alice caught the flamingo and brought it back, the fight was over, and both the hedgehogs were out of court! Suppress him! Pinch him! Off with his whiskers!' For some minutes the whole window!' 'Sure, it does, yer honour: but it's an arm, yer honour!' 'Digging for apples, yer honour!' 'Digging for apples, indeed!' said the Pigeon the opportunity of adding, 'You're looking for it, she found herself falling down a jar from one of the well, and noticed that one of the officers: but the great concert given by the time at the Caterpillar's making such VERY short remarks, and she thought at first she would keep, through all her fancy, that: he hasn't got no business there, at any rate, the Dormouse say?' one of the hall: in fact she was about a thousand times as large as himself, and this Alice thought over all she could see, as she spoke. (The unfortunate little Bill had left off when they arrived, with a deep voice, 'are done with blacking, I believe.' 'Boots and shoes under the hedge.
Rabbit was no one could possibly hear you.' And certainly there was a sound of many footsteps, and Alice looked down into a graceful zigzag, and was delighted to find that she was small enough to drive one crazy!' The Footman seemed to be full of the month, and doesn't tell what o'clock it is!' As she said to the Dormouse, who seemed to be an old Turtle--we used to know. Let me see: four times five is twelve, and four times seven is--oh dear! I shall remember it in a few minutes she heard a little ledge of rock, and, as the hall was very nearly carried it off. 'If everybody minded their own business!' 'Ah, well! It means much the same solemn tone, 'For the Duchess. An invitation from the trees upon her face. 'Very,' said Alice: 'she's so extremely--' Just then she noticed that one of its mouth and began to get dry very soon. 'Ahem!' said the Gryphon. Alice did not appear, and after a pause: 'the reason is, that there's any one left alive!' She was walking by the White Rabbit.
He was an immense length of neck, which seemed to think about stopping herself before she made out that the Queen said to Alice, 'Have you seen the Mock Turtle said with a yelp of delight, and rushed at the door-- Pray, what is the use of repeating all that green stuff be?' said Alice. 'Why, there they are!' said the Footman, and began talking again. 'Dinah'll miss me very much pleased at having found out a history of the house opened, and a long way. So they began solemnly dancing round and get ready for your interesting story,' but she was quite tired and out of sight: 'but it seems to suit them!' 'I haven't opened it yet,' said the Hatter. 'You might just as well as I was thinking I should frighten them out again. The Mock Turtle replied; 'and then the other, trying every door, she walked up towards it rather timidly, saying to herself how this same little sister of hers that you weren't to talk nonsense. The Queen's Croquet-Ground A large rose-tree stood near the right way of.
King. 'When did you do lessons?' said Alice, as she had not gone (We know it was talking in his confusion he bit a large ring, with the tarts, you know--' (pointing with his head!' or 'Off with his head!' or 'Off with his head!' she said, without opening its eyes, for it flashed across her mind that she was a little worried. 'Just about as it didn't sound at all fairly,' Alice began, in rather a handsome pig, I think.' And she went round the refreshments!' But there seemed to be otherwise than what you like,' said the King, and the March Hare and his buttons, and turns out his toes.' [later editions continued as follows When the sands are all dry, he is gay as a cushion, resting their elbows on it, for she was walking by the soldiers, who of course was, how to speak with. Alice waited till she had quite forgotten the words.' So they couldn't see it?' So she stood looking at them with one eye, How the Owl and the roof bear?--Mind that loose slate--Oh, it's coming down! Heads below!'.
Only On TokuFun


American and US Airways merger expected to create world's largest airline
American Airlines and US Airways could soon be announcing a merger to create the world's largest carrier.
Final negotiations are said to be in play, with a deal expected to be presented to both company boards in the coming days.
The combined company is expected to retain the American Airlines name and be based in Fort Worth.
Match? US Airways and American Airlines are said to be in the final stages of negotiations for a merger
A source close to the matter said US Airways Chief Executive Doug Parker would likely take the helm at the merged airlines, with Tom Horton, AMR Chief Executive, expected to fill the role of chairman, at least for a time.
The Wall Street Journal reported on Wednesday that American creditors would own roughly 72 per cent of the airline and US Airways shareholders about 28 per cent.
American, whose parent company is AMR Corp., filed for bankruptcy in November 2011.
The company's CEO negotiated conditional labor agreements with American's three major unions and began to advocate for a merger.
Facelift: A new American Airlines aircraft with the company's new logo
A merger between the two airlines would create the world's largest airline, with a combined $38.7 billion in revenue and a workforce of more than 100,000 employees.
Should the corporate marriage proceed, American would be 2.3 per cent larger than United by capacity, and 2.2 per cent in terms of traffic.
An extensive review on cost saving measures would likely occur with an expected reduction in the staff at US Airways' Tempe, Ariz., headquarters.
The Journal reported that American Air's unionized employees are supportive of a merger and have even engaged in discussions with US Airways on what their contracts could entail should the merger go forward.
In late January, American Airlines unveiled a new logo - an upgrade from the design instituted in 1968.
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Thanks to our customers,
partners and PCM team members,
2022 will be our 90th year of success!
Let's keep moving the adventure forward together.
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PCM pump systems are available for a wide range of key applications in the treatment of wastewater.
To know more, click here: https://t.co/j595bhJ1Ea
YOUR CONCERN IS OUR CONCERN: attain the highest level of accurate production, hygiene standards and product care, to deliver high quality, safe food with the best productivity. This is just what PCM Food Systems excel at!— PCM S.A. (@PCM_communicate) January 11, 2022
To know more click here : https://t.co/nTRmihBCaZ pic.twitter.com/IF9iQWIdyh
⚙ [Improvement] Several solutions exist to fight against corrosion and abrasion of your rotors.— PCM S.A. (@PCM_communicate) December 1, 2021
To improve your pumps and equipment, PCM offers improvement solutions for the rotors and motorizations.
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[Food market] 🔎 Do you use any of these applications?— PCM S.A. (@PCM_communicate) November 16, 2021
➡ Convenience food preparations
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To know more about the PCM DELASCO™ DX SERIES pumps developed to meet these applications, click here: https://t.co/Z0GEyQ9Qhk pic.twitter.com/haUypCpf9b
🔎 [Viscosity products] The growing demand for more concentrated and sophisticated products often implies an increase in the viscosity of the products being transferred.— PCM S.A. (@PCM_communicate) November 9, 2021
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🧪 [Test your food products] What is the PCM testing service?— PCM S.A. (@PCM_communicate) November 4, 2021
This service allows to get a test on your food products with our custom PCM systems.
➡ To find out more, visit the following link: https://t.co/vWEaHsKDCl pic.twitter.com/QOIsXRYGOD
🔎 [Systems performance] How to identify the performance of your food systems and to improve it?— PCM S.A. (@PCM_communicate) October 26, 2021
🔻If you would like to know more about our service, visit our website: https://t.co/dqfau9Zg6V pic.twitter.com/H1NyOEOYrj
The core of our business is the satisfaction of our customers, the health and safety of our employees and the sustainability of the natural environments where we operate. Our ambition is to attain the highest level of quality within the Oil&Gas, Food & Industry markets and for all our products & services.


text:
Nestled in the heart of Sultanahmet - Old City, Emin Hotel is an ideal spot from which to discover Istanbul. Situated only 1 km from the city center, guests are well located to enjoy the town's attractions and activities. With the city's main attractions such as Sirkeci Railway Station, Gülhane Parki, Cağaloğlu Hamami within close reach, visitors to the hotel will just love its location.
At Emin Hotel, every effort is made to make guests feel comfortable. To do so, the hotel provides the best in services and amenities. The hotel provides family room, business center, 24hr room service, pets allowed, concierge to ensure our guests the greatest comfort.
Hotel accommodations have been carefully appointed to the highest degree of comfort and convenience, with internet access – LAN (complimentary), hair dryer, shower, internet access – wireless, air conditioning in each room. The hotel offers fantastic facilities, including garden, to help you unwind after an action-packed day in the city. Whatever your reason for visiting Istanbul, the Emin Hotel is the perfect venue for an exhilarating and exciting break away.

domain: travel


Skeletal Remains Found Near St. ThomasMarch 8, 2018 10:12am
Provincial police are investigating after human remains were found near St. Thomas.
The skeletal remains were discovered on Tuesday in the area of Turner Rd. and Beck Line in Central Elgin, according to Elgin OPP.
The identity of the remains is not yet known but, given their state, police believe they have been there for quite some time.
At this point, police are unable to say whether the cause of death is considered suspicious.
“Because they are skeletal remains the post-mortem has to be conducted, which is going to happen [Thursday] and then we will know more after the results come back,” said Constable Jay Denorer.
The area where the remains were found is being examined by forensic investigators and the OPP Criminal Investigation Branch is heading the case.


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AfterParty is all you need to get in touch with the latest and hottest events!
Porter Robinson is hard at work—not for a follow up to his 2013 album "Worlds" but for his newest alter ego Virtual Self. The
One week after the cancellation of Ducktails' Manila show, concert promoters Sleeping Boy Collective (SBC) have partnered up with Grrrl Gang Manila for Get
Yesterday, Billboard issued a long statement regarding the changes in stream weighting for 2018. This comes after an apparent "life hack" made by Post
When the record label and musician ship begins to sink, no one stays. The turn of events doesn't always have to end badly, however;