Enjoying the fine weather was Ian while working on the brake hanger for one of the two refurbished Commonwealth bogies due to go under TSO 4986.
New pipes were literally delivered that morning - very timely to say the least! John measures a length prior to using the power saw.
As new heating pipework it was soon being installed under BCK 21092.
Ken Austen carefully cleans up another window corner section prior to welding this in on the Malvern side of BSK 34929
Dave Hancox priming one of the sanded down battery boxes from BCK 21092.
Yes, it's Malcolm doing the roof once again, but of course no longer on it. The Cotswold side of TSO 4986 receives its first top coat of Flint Grey.
Alan Baugh and, at the far end, Bob Slater were on the Malvern side of the coach. Still a fair amount of filling and sanding to sort out, most of it on the window frames where these were removed.
While Paul was sanding down more of the beading strips for 4986,
Bob Keyte was busy giving a second coat of varnish to the large number already done. In the background are new primed steps for DMU 51405
With the new ceiling panels for 4986's south vestibule recently primed, Richard Hoy now adds white undercoat.
Back to Bob K. again straining the top coat for the final three of the seat side panels for 4986.
Back down to the north end of the Paintshop where Phil Salter is reinstating the electric lighting in the Fruit C van.
Just a few yards away Phil Jones continues the cleaning and repainting in the cab of the DMU.
In the north compartment the Indoor Gang were back on the windows. Tony adds primer to a completed one on the Malvern side.
Alan was fitting the final section of framing in the next one on the same side.
While on the opposite side Pat was installing the sections of underframe.
Also on the construction side was Bryan, fitting the final section of missing capping strip on the Cotswold side south end. Once the filler has been rubbed down, this panel can be given its first top coat.
Cheryl and Jeff (not photographed) were cleaning the door handles and stops.
Richard was cleaning the back panelling on the doors. This made quite a difference with the panels slightly gleaming from the polish. They will be given a final rubdown at the end of the refurbishment to remove any polish bloom.
No, he hasn't disappeared yet. Last, but definitely not least, Alex brightens up the connection panelling with some fresh paint.
Report by Dave Clark


text: This playlist is about music discovery. Here bring you great music that your ears probably has not heard. This playlist contains the best songs from upcoming artists, with some familiar names added in there for good measure. A few songs we missed from February are on there too. Enjoy the best of March below and discover something new.

domain: artistic


Among the many cultural artifacts that COVID-induced isolation has led Americans to rediscover, the “Why I Left New York” essay is becoming the most common.
The exodus from Gotham and other cities is grist for newspapers and magazines and blogposts and Medium brimming with once committed Cobble Hill residents who now delight (or despair) about moving to Chatham or Cos Cob. And while it may be too early to know if these trends are permanent, FlatRate Moving reported moves from New York to Connecticut increased 74 percent between March and April of this year as compared to last year, while New Jersey and Long Island saw 38 and 48 percent spikes, respectively, per the New York Times.
The logic of saying “Bye” to Brooklyn is fairly straightforward — in an era of social distancing, why pay astonishing sums for cramped quarters, a greater likelihood of contracting the virus, at least initially, and no ability to frequent the events, nightlife, and cultural activities that make America’s cities worth living in, not to mention a crime spike, more homelessness, and a school system that shuts down despite low transmission rates in many districts? Suburbs, once shunned as boring, conformist, with bad casual dining restaurants, have begun to look better in recent years. Some of the cool families were already crossing the Hudson to Montclair or Maplewood. Now, everybody, it seems, is heading for the PATH trains and Metro North.
The questions now are whether this migration is temporary and will be swiftly forgotten when a vaccine is distributed — or is it more enduring? If there’s more Zoom work and less advantage to living near the (now empty) office tower, then why stay in Williamsburg even when the pandemic ends?
It’s also important to know that the suburbs’ mythic underpinnings — homeownership, the nuclear family, and sprawl — are changing as American priorities change and suburban tax bases are reconfigured.
“The culture that created Levittown doesn’t exist anymore,” said Brian O’Looney, an architect of sustainable urbanism and a panelist at the National Press Foundation’s “The Suburbs: After COVID, A Changed Landscape?” program last week.
In the American imagination, the suburbs are conceptualized as high-income, exclusive, white spaces with great schools, the Pew Research Center’s Richard Fry said demographic and economic data show a different pattern. Over the last 20 years, suburbs have become more diversified, both ethnically and economically.
Large suburban counties have gained 17 million people between 2000 and 2018, Fry said, outpacing cities’ growth. But when broken down by age group, the cities still retain a growth advantage in one notable demographic — people aged 25 to 44, or those in their prime working years. With growth concentrated on either side of the age spectrum, suburbs are expected to provide a strong education and healthcare infrastructure, but without the prime-age workers. They do not necessarily have the tax base to support that. Poverty has grown at a sharper rate in large suburban counties than in urban or rural areas, while average household incomes have fallen in the suburbs since 2000. Property values are appreciating more slowly than in urban cores, or at least they were until this recent panic.
“On any number of economic outcomes, what we see is suburban areas are lagging what we see in the urban core,” Fry said.
If COVID trends hold, suburbs could attract the 24-to-44 age demographic. But the suburbs, which were designed around the ideas of owning a car and segregation, both racial and economic, do not resemble the urban neighborhoods that these working professionals prefer. That mismatch could facilitate what June Williamson, a professor of architecture at the City College of New York and the co-author of a forthcoming book in her “Retrofitting Suburbia” series, calls much-needed “incremental metropolitanism,” where suburbs are remade to be more sustainable and resilient.
Williamson proposes three strategies for retrofitting suburbs to meet new cultural and economic realities — “reinhabitation,” in which abandoned and struggling office parks and big box stores are adapted for residential and mixed-use, redevelopment, which includes new construction to maximize walkability, density, and transit, and re-greening, to restore wetlands ecologies and wildlife corridors.
“Certain trends that drive the retrofitting of suburban form have been going on already for some time, and the pandemic seems to be accelerating and intensifying some of them,” Williamson said, listing the death of the shopping mall, vacated office parks, and bankruptcies as examples. “These losses are painful at the local level — loss of tax revenue, jobs, and the eyesore properties that are left beyond. (They are) also places of opportunity, especially for well-located sites in high growth and transit-friendly areas.”
Suburbs’ long history of exclusionary zoning was designed to keep property values high, incentivize homeownership, and, often to maintain racial segregation. But values have changed — subsidized affordable housing has become a priority and a necessity, particularly in inner-ring suburbs. Younger millennials are much more interested in renting than owning. Older people often want to downsize while staying in the suburbs. Meanwhile, the middle class is often priced out.
To address these challenges, Williamson said the suburbs need zoning reform — and expressed hope that the new suburbanites who moved post-pandemic might be incentivized to incite change. As downsizing and renting become more popular, creating new developments to accommodate multigenerational households, home rental, and retrofitting commercial property to meet the needs of seniors — for example, building cottages around a central green on an acre of property — would open up opportunities that meet current needs.
O’Looney said creating these alternative lifestyle developments can be difficult in a country like the U.S., where so much of land use control is local when efforts, particularly around affordable housing and environmental policy, can be more efficiently solved at the state level.
He also said the U.S. lags in housing affordability, particularly due to the expenses associated with cars, such as having to build multiple parking spaces for single-family homes. If they ever appear, new transit opportunities could improve affordability and help give suburbs a more modern character.
If suburbs can be made more affordable, accessible, and livable, it may even ease the urban pressures that lead to gentrification, Williamson said.
Politically, suburbs are beginning to look more like cities as well. President Trump’s appeal to suburban voters based on the notion that Democrats would bring crime-plagued, low-income housing to your neighborhood didn’t help him in the big suburban countries that ring America’s cities.
“That whole political narrative was like shadow boxing with a ghost of this persistent stereotype of what suburbs were in the past,” Williamson said. “It had some traction, but it actually led to more activism in certain pockets to say ‘That’s not what the suburbs are today!’”.
“As (millennials) get older, maybe they’re going to move to those inner suburbs because of the prices…If it’s near a transit stop, if there’s a coffee shop, if there’s a different kind of townhouse they can buy or some older house they can remodel and work from home, they’re going to remake those suburbs.”


The letter states that foreign capital must return nearly $9 billion in Central Bank of Afghanistan assets to Da Afghanistan Bank (DAB) for the economy to function, despite criticism of the ruling Taliban’s behavior toward women and minorities.
“The people of Afghanistan have suffered doubly because of a government they did not elect,” the letter said. “To mitigate the humanitarian crisis and put the Afghan economy on a path to recovery, we urge you to allow DAB to restore its international reserves.”
The letter, also addressed to US Treasury Secretary Janet Yellen, was signed by 71 economists and academics, many of them from the US, as well as Germany, India and the UK. Among them were former Greek Finance Minister Yanis Varoufakis and Stiglitz, a Columbia University professor who was awarded the 2001 Nobel Prize in economics and a member of the advisory board of the Washington-based Center for Economics and Policy Research, who wrote a structured letter.
The Afghan economy has been in deep crisis since the Taliban took power nearly a year ago when foreign forces withdrew. The sudden drop in aid and other factors, including conflict-induced inflation in Ukraine, have contributed to this, but economists say the country faces severe hurdles due to the inability of its central bank to operate without access to its reserves.
This led to a sharp depreciation of the Afghani currency, driving up import prices, and almost collapsing the banking system, as citizens had difficulty accessing their savings and receiving their salaries.
“Without access to its foreign reserves, the Central Bank of Afghanistan will not be able to perform its basic and basic functions… Afghanistan’s economy has collapsed as expected,” the letter read.
Washington and other capitals say they want to find a way to release the money for the Afghan people and not the Taliban, who have condemned it for severely curtailing women’s freedoms over the past year and are reported to be violating human rights, including through retaliation against them. former enemies.
The Taliban says it respects rights according to its interpretation of Islamic law, and that individual abuses will be investigated.
Despite widely diverging positions, the two sides are in detailed discussions about plans for the eventual release of central bank assets, of which nearly $7 billion is held in the United States. About half of these are currently being set aside as the subject of a lawsuit related to the September 11 attacks.
The main sticking points remain in the banking talks, especially with regard to US objections to the appointment of the Taliban deputy governor of the Central Bank, against whom US sanctions have been imposed.
Avid music fanatic. Communicator. Social media expert. Award-winning bacon scholar. Alcohol fan.


text:
by Mindy Aguon
Guam - A plea agreement couldn't be reached in Kyle Indalecio Camacho's criminal case and he'll be going to trial. Camacho is charged with kidnapping a woman from the Micronesia Mall in Dededo back in January. He remains behind bars.
Previously there was discussion about a possible plea deal, but court officials tell KUAM News the trial is expected to begin on Wednesday before Superior Court Judge Anita Sukola.

domain: law


text:
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Cotton jersey. Slightly stretch for comfort. American matter and manufacture. Round neck with added band. Inside seam trimmed in the neck. Typographic motif "NEW YORK CITY APC" silkscreened on the chest. Short sleeves. Stitched hem at the bottom of sleeves and T-shirt. Claw "APCUS Made in California, USA" silkscreened on the inside of the back.

domain: design


text:
If you have speakers already connected to your stereo, fully disconnect them. Active crossover units are larger than passive ones and need to be mounted in a stable location, ideally near your amplifiers. Using the appropriate cables, wire your crossover into the receiver or preamp, with the wires coming out of the receiver going into the “in” terminals on the crossover. Depending on your receiver and your stereo system, you will probably be making this connection with RCA cables, but some crossovers will are set up to use speaker wire instead as detailed in Method 1 if your receiver doesn’t have RCA outputs. Using the appropriate cables again, usually RCA or speaker wire for a home stereo system , connect the appropriate outputs to the appropriate amplifiers. If you don’t have a subwoofer, you’ll send your high frequency signal to your tweeter amp and low-frequency signal to your woofer amp. In this case, make sure your crossover is set to two-way mode. There should be a switch that controls this. If you can’t locate it, check your instruction manual. Connect the left output of each frequency range to the left input of the coinciding amplifier and the right output of each frequency range to the right input of the coinciding amplifier.
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How do you hook up a 1 farad capacitor to your amp? Answer 1 You first have to determine which side of the cap is positive andwhich side is negative. You run a wire from the negative side tothe chassis and …for the positive side you run a cable from thebattery to the terminal and from the terminal to the amp. Be careful not to mix up positive and negative on the capbecause it could explode and cause damage.
Answer 2 Because the capacitor is used to filter changes in supply voltageto the amp, the capacitor should be as close to the amp aspossible, and connected with heavy wiring. The capacitor also actsas a huge reservoir to supply lots of current as the amp signalhits high levels.
Check out the latest damaged amp courtesy of the UPS gorillas. This was a wonderful-sounding Premier B Club Bass, an especially good sounding Blues amp for guitar. Original â€™65 Jensen C15N is totaled beyond repair, speaker baffle is in many parts.
They are the same thing. So the M13 is a pretty great piece of gear because you get over different stompbox style effects in one big, road-rugged container. The M13 requires that you plug it into an amplifier. It may sound pretty raggedy going straight into a PA, mixing desk, high end microphone preamp, audio recording interface, etc. And aside from the modeling vs. That is beyond brilliant. By tapping the tempo button along with your drummer, you can set 1 delay to a dotted 8th note, another delay to a whole note, a vibrato patch to triplet 16th pattern, and a filtered cosmic gurgle slurper to a half note.
You can see that there are stereo jacks for input, output, fx send, and fx return. The location or placement of your fx loop can be assigned PER SCENE from the setup menu which means that in one scene, you can run one bank of effects into the front of your amp and the remaining three banks each with three effects selections into the loop.
Or you can place the loop between banks 2 and 3 and have two banks into the preamp and two banks into the loop. The graphic below gives a visual of what that looks like:
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I RV was kind enough to include one in the start-up kit they furnished. They cost a minimal amount, are a necessity and in our case was furnished by the dealer. Dog Bone Adapter lets you hook up to 15 amp outlets. Weatherproof jacket encloses 10 gauge, 3 wire cord for safe, free-flowing AC power. Molded plug and socket.
: hook up ipod to car stereo. Weatherproof Speaker And Amplifier Sound System, Two 3 Inch Speakers, Bluetooth Amplifier, Inline Volume Control, Ideal For Motorcycles/ATV and 12 Volt Applications. by BOSS Audio Systems. $ $ 66 11 Prime. FREE Shipping on eligible orders.
Just what is tone? What is a good tone and how do you achieve it? Which amps you should choose for your setup and how to get the tones you want. We also looked at the importance of choosing the right guitar — not only for your technique and rig but also for inspiration to play and create music. Check out part 1 and part 2. Amps People tend to prioritize guitars and a bunch of pedals and forget the importance of a good sounding amp.
Choosing the right amp for your setup is crucial for getting the tones you want from your guitar and pedals.
Amp,transceiver, tunner hook up.
AMP gauges at the dash are troublesome. They should be by-passed, and then install a VOLT gauge. With normal but frequent use, most of these Dodge trucks will have electrical wiring problems.
May 05, · Next its time to hook up the speaker wire from the sub to amp. In this case the wires are blue. The blue wire with the white stripe is positive and the solid blue is negative then hook up logically!
Share on Facebook Graphic equalizers became popular in home audio applications in the late s and throughout the s. They can be easily recognized by their series of slide controls that divide the frequency generally from 20 Hz to 20 kHz, the approximate range of human hearing. The slide controls are centered at zero and slide up and down from zero to 3 db. Hooking up a graphic equalizer to a stereo receiver is a relatively straightforward and simple process.
Graphic equalizers allow you to fine-tune your audio experience. The units came from the factory with easily removable RCA connectors in place. To hook up a graphic equalizer to such a unit, remove the plugs and connect one pair of audio cables to the pre-amp output and the other end into the left and right channel inputs on the rear panel of the graphic equalizer. Plug a second pair of audio cables from the left and right channel inputs of the amp to the output channels on the back of the graphic equalizer.
Turn on both units and adjust the equalizer slide controls to your preferences.
How to Install a Car Amp
You can hook up a car amp to a battery charger inside your house and use it as a glorified power source for varied needs. Snip about inches of blue wire, leave the metal strips at each end bare; cut away the rubbery portions. Hook one end of this to the 12V node. Hook the other end to the REM node, sitting adjacent. Take the battery charger in hand now; this is your power supply source.
How to Hook Up a Comcast Cable Box. This wikiHow teaches you how to connect a Comcast cable box to your TV. Make sure that you have cable installed. If a Comcast representative didn’t come to your house to install cable, you’ll need to.
Hey all, Long time reader, first time poster – please go easy on me! Here is my question: Can I hook up a 1×12 combo amp to a 2×12 cabinet and use all three speakers? I’ve done a lot of reading on this subject online and there are of course many forum threads that are similar to this question, but I can’t seem to synthesize all of the general information to educate myself on this specific scenario, so I thought I’d pose it to this community. The specific combo amp that I have is the Carvin Nomad 50 watt, 1×12 inch speaker.
It has a Celestion Vintage30, 16ohm speaker. The amp has two speaker outs obviously one is being used by the combo speaker , and it can be toggled between 4, 8, or 16 ohms. What I’d like to do is get a 2×12 cabinet to use as an extension cabinet for this combo, but I want to use the combo speaker and cabinet simultaneously. I haven’t yet bought a cabinet, as I’m trying to figure out what the specs of the cabinet wattage, ohms of speakers, wired ohms, etc need to be to work for my purposes.
Budget really isn’t a consideration for me at this time. Thanks so much everyone.
Where to hook up “remote” wire for amp
Opt out or contact us anytime Mr. In his nearly four years in office, he has not pressed for expanded gun control. But he did allude on Friday to a desire to have politicians put aside their differences to deal with ways to prevent future shootings. It had a security protocol that called for doors to be locked during the day and visitors to be checked on a video monitor inside.
A double-pole breaker is needed whenever you’re doing volt wiring. It consists of a pair of breakers arranged side by side so they contact alternating bus bars. To install one, attach the hot wires to the breaker, the neutral wire to the neutral bus and ground wire to the ground bus.
When jumpstarting a car you essentially give the dead battery just enough boost to start the engine and then rely on the alternator to charge the battery the rest of the way. When using a battery charger, you allow the battery to charge all the way before it will be used again. This should answer important questions such as what the voltage requirements are for charging your battery and whether or not you should remove it from the vehicle before charging.
There will be a manual with your battery charger that provides details for the appropriate use of the charger. Working in a well-ventilated area will help dissipate the hydrogen gas that batteries generate from the sulfuric acid within their cells. Also, make sure to keep any other volatile substances, such as gasoline, flammable materials, or ignition sources flames, cigarettes, matches, lighters away from the battery at all times.
Glasses, gloves, and other protective gear is a good precaution. Hydrogen released within the battery quickly converts to a gas and expands, this can cause the battery to explode if venting mechanisms fail. These accessories draw power from the battery and should be turned off before removing or charging the battery.
Most batteries will be found either under the hood of the vehicle or in the trunk. It is also possible that your battery may be under the rear seat, and sometimes the battery can only be accessed from underneath the side of the car. There are a few ways to tell which is which: Compare the diameters of the battery posts.
Definition of hook up
June 05, , Nowhere in the Extra Class study manual did it tell which amps work with which rigs, and how to connect them without letting the smoke out of something. Because that can happen. By looking at the schematics and with help from others, I did pick up a few things. Inside my FL amp there is a relay that has to get energized in order to route the RF input signal through the amp and connect the resulting signal to the antenna, and also to tell the amp to wake up and amp.
When this relay is not energized, the RF goes straight through to the antenna with no amplification and the amp is in standby.
Jan 07, · Ok, here someone out there has knowledge on hooking up a new Kenwood Ts to an old SB amp. I contacted Kenwood about this and they said that the radio will actuate a relay that draws ma or less at v.
Originally Posted by dirtinla I understand how to wire up the 3 wire alternator. The number 1 wire goes to a resistor or dash light and is suppose to glow when not charging. That wire goes to a dash light. The Dash light has 2 terminals one for neg and one for positive. This is my question: When I plug it into the light I need to know if the other connector goes to ground or hot pos. It will have two wires coming from it. It does not make any difference which wire connects to the 1 wire coming from the alternator.
The other wire from the socket connects to the ignition terminal on the ignition switch.
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The mixer is an integral piece that takes the sound being picked up by microphones and processes it to be sent to the powered amplifier and then the speakers. Peavey offers a full line of professional live-sound mixers for use with small and large PA systems alike. Follow a few simple steps and you will have your mixer up and running in no time.
QUESTION: I have a question on the setup of a Decimator G Sting Pedal. I’m trying to use the decimator in the effects loop and in front of the amp.
Supplies Gather your supplies. For the sake of this build we are going to assume you have a 30 amp plug on your generator – like the one shown. If your have one different please adjust your supplies. Buy a UL rated device that fits your specific breaker box. These have been tested and validated to work. Many insurance companies and jurisdictions require the UL rating. Again you will need to buy one that fits your breaker box.
All breakers are not the same. I bought 10 feet of 10 gauge wire in black, red, green and white. Schedule 40 electrical conduit and fittings Conduit body Flexible Non-Metallic Conduit and fittings optional 30 Amp generator extension cord. Search Amazon for your plug type extension cord. Notice how all the amperage match. If you are using a 50 output on your generator you will need a 50 amp breaker, 50 amp power inlet box, a 50 amp extension cable and 8 gauge wire.
Add Tip Step 4:
Definition of hook up
THD Hot Plate 2. Offers Bright and Deep switches for tailoring your sound – Bright switch gives you two different high frequency levels to compensate for an overly bright, or dull speaker cabinet while the Deep switch offers two distinct bass settings to help you fill out the bottom end, or reduce the bass in a cabinet with too much low end. Has built in noise reduction up to 10dB, line out, and a fan to keep it cool.
For full specs click here for THD’s site.
May 15, · You just hook the ‘s L and R pre-outs (assuming your receiver has them) to the L and R RCA inputs opinion the amp. You then run the speaker wires to the appropriate speakers. You might need to go in to your AVR’s menu and asign the front channels to an external amplifier, not familiar with the though.
View WillK’s Album Ok, this sounds a lot like part of my project but I’m using an 8 space load panel where I’m using a 20 space panel And using smaller cable, I’ll get to that. First of all, it looks like you’ve probably got Square D Homeline? Just wanted to check because they have 2 lines, one is Homeline and the other is QO. I’m using a Homeline panel so if that’s what you have then I can refer to my own stuff.
Also, I saw they have 8 space main lug and main breaker, this needs to be main breaker so you have a main shutoff in the garage. Your original question is a little unclear if you’re asking about the connections at the house end or the garage end, it sounds like you are asking about the garage end, but it also sounds like you need guidance for both ends. First, it sounds to me like you have black and black with gray stripe which should be your two hot conductors. White should be neutral. Green should be ground.

domain: hobby


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June 30, 2005
The Bicycle Transportation Alliance is searching for 3000 donuts. At a recent staff meeting, our Program Director Brita Johnson asked if we knew where to find 3000 donuts. That’s right, 250 dozen.
Well, Voodoo Donuts has their Tex-Ass Sized donuts, but asking them to make 3000 cool donuts isn’t a likely winner. Perhaps Franz Bakery, whose lovely smell of baking permeates blocks around their factory at Northeast 11th and Everett, is the place to go. I can also imagine a Krispy Kreme poster of a bicycle whose wheels are made out of donuts. But if you’ve got ideas, let us know.
Why do we need so many donuts? Well, it’s a summer of fun for bicycling, and one of the events we help with, and benefit from, is the Midsummer Night Ride, July 9th. It’s a great ride through the city in the middle of the night with thousands of other cyclists. For those of you who may not have ridden in the middle of a summer night much, it’s an amazing experience, especially with a few hundred friends. Seriously consider going.
Or if you want to help with any of our events this summer, we’d love your help.
Otherwise, find us some donuts, will you?
June 29, 2005
What do global warming, the end of "cheap oil" and the Legislature's refusal to raise gas taxes the 12th session in a row have in common? Together they are creating the perfect storm for transportation as we know it.
Storms sink most boats but they also give rise to great surfing for those who anticipate and are prepared to ride out the waves instead of fighting them. Every community in the US is facing the same storm in one form or another. No place has enough money to build its way out of congestion, and Portland is no exception (compare congestion numbers at the Texas Transportation Institute’s website).
No place except maybe Texas has oil to meet its local needs (US production peaked in 1970's and the world production peak is fast approaching) and burning all this fuel to accomplish errands we could do on foot, by bike or on transit is having major impacts on the survivability of our species.
How do we keep commerce flowing if the roads are clogged with commuters? When gas goes to $5 a gallon what will people living in north Clark County (where they can still buy a 1 acre lot in sprawlurbia) and commuting to Hillsboro do? Can bikes really save the world? If everyone wants to live in the city, where will they live? And just as fundamental to our sustainability, where will people of lesser means live if the well-to-do continue to bid them out of their conveniently located homes?
These are themes that I will be exploring in future contributions to this blog. Stay tuned!
For the last 30 to 50 years, transportation planning in the United States has been:
1. Carried out largely in isolation from regional, city or neighborhood land use planning, even though we know transportation investments shape property values and land uses and land use regulations play a major role in travel patterns.
2. Based on unexamined assumptions about what are the right questions to address. (For example, is the right question always "How do we reduce congestion on this corridor?")
3. Shaped by an environmental impact statement analysis that often uses straw-men alternatives (especially the "no build" alternative) and that are organized around analyzing a single "corridor" instead of more sophisticated combinations of transportation investments, land use strategies and demand management.
4. Powerfully shaped in Oregon (and elsewhere) by state and federal mandates limiting the use of funds for only certain kinds of transportation investments.
5. At its worst, one of the last refuges of back-room deals and log rolling by a narrow group of interests, conducted out of the public eye.
Our state and region have made progress in changing some of these dynamics, especially #5. But the time is right to take new steps in this region to reform how we make these decisions that use so many tax dollars and have such a profound effect on our region and neighborhoods.
I would welcome other peoples' ideas about what reforms are needed and how we can gain acceptance for change.
June 28, 2005
Welcome to "a conversation about mobility" in our region.
The goal behind Portland Transport is to let the thought leaders on transportation issues in the region converse with each other, and with the general public.
We have assembled a diverse group of contributors, and will be adding more as the site grows. They include elected officials, agency staffers and transportation advocates. If you'd like to contribute, let us know. This is definitely a group effort.
We operate under a simple set of rules, and welcome your thoughts on how we can improve the site.
June 1, 2005
Listen to the show (mp3, 13.4M)
All about Shift's 2-week bike festival.


SHEPPEY WIN WHISTLER TROPHY
On Wednesday night, Club Chairman Neil Golding was presented with the Kent Society of Rugby Football Union Referees Whistlers Trophy by new President, Graham Charters. Sheppey are in the first winners of the modern era of this award and Neil Golding eagerly accepted it on behalf of the Club. Throughout the season Kent refs have judged each club on how they welcomed referees and looked after them and at the end of the season, Sheppey were streets ahead of the rest. Chairman of the KSRFUR, Terry Hall, made tribute at their AGM of how welcoming a club Sheppey is and how it is always a pleasure to go there. Terry hall, a national level referee himself, said that the award is fully deserved.
Neil Golding commented, "We are delighted to be awarded this. The plaque says 'Sheppey RFC - the most welcoming Club in Kent', what a fantastic thing to have said about our club. We do make sure that every ref is well looked after and I thank the bar staff and Director of Rugby, Andy Hosken in particular for their lead on this. Without a ref we have no game so it is vital that they enjoy their experience of coming to Sheppey."
Clearly now the challenge to the Club is to maintain the standard and win it again next year!


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text:
Mashups using the same API (2678)
New York City is probably best known for its skyscrapers and millions of people, not the trees that line its avenues. A new iPhone app goes out on a limb to give a little credit to the oaks and maples. And it's doing it thanks to open government data.
Sleep tight and don't let the bed bugs bite. This is a cute saying if you have never had the misfortune of actually letting the bed bugs bite. But a quick Google image search shows that these little critters are no fun at all. A Yahoo Maps mashup is trying to help you find them before they get you.
Urban Algorithms Geo Data
||The Urban Geo Data API allows users to access socio-economic and environmental profiles of specific GPS locations. Such data types include: air and noise pollution, crime rate, demographic profiles,...||Data||10.17.2016|
||The FINOU Maps API is an interactive Moroccan Mapping service that can be integrated into websites and applications. The sets of services offered include: map tiles service, geocoding service and...||Mapping||09.22.2016|
MapQuest Real-Time Insights
||This API allows clients to receive GPS observations generated from mobile applications using the MapQuest Tracking SDKs on a second by second basis called MapQuest Observer. It is comprised of three...||Mapping||09.19.2016|
||This API allows clients to receive GPS observations generated from mobile applications using the MapQuest Tracking SDKs on a second by second basis. It provides a way for clients to curate the...||Mapping||09.19.2016|
MapQuest Data Manager
||This API allows you to store custom datasets in a spatially-aware database that resides on MapQuest servers. This includes tables that contain points, lines, and polygons that are stored as...||Mapping||09.19.2016|

domain: geography


text:
Dr Anna Ball, with the PAMOJA Women Together Group at Nottingham Refugee Forum.
‘And Still I Rise’ is an interdisciplinary outreach project that explores the benefit of creative activity, discussion and self-representation with the PAMOJA Women Together Group at the Nottingham Refugee Forum: a group of around 30 women and their children from a range of asylum-seeking and refugee backgrounds. The project is led by Dr Anna Ball, Senior Lecturer in English and Co-Director of the Postcolonial Studies Centre, and is run in collaboration with Nottingham Contemporary, who have hosted the group through their Education and Outreach programme. Over the course of seven weeks (May-June 2018) and working with a team of volunteer translators, as well as NTU student photographer and filmmaker Rasha Kotaiche, and performance coach Iryna Muha, the group are exploring their responses to the inspirational work of African American poet Maya Angelou, in order to generate their own ideas about and representations of women’s resilience, strength and value. The group has developed skills in poetic analysis, creative writing, photography, performance, filmmaking and art, through which the group members have been able to explore varied platforms for self-representation, and to articulate the value of creative expression as a mode of agency and solidarity. The project will culminate in a multi-disciplinary exhibition of photography and poetry at Nottingham Central Library and a two-day pop-up exhibition at Nottingham Contemporary, during Refugee Week in June 2018. It is hoped that the written work generated by the project might be developed into a poetry anthology published by Palewell Press.

domain: sociology


text:
Every couple faces several challenges and humps along the way of marriage. Yet a successful marriage usually takes work, as any relationship does indeed, and it’s extremely important to keep a positive perspective and stay committed in the face of issues.
In spite of what we could have heard in the movies or from your friends, powerful marriages no longer happen overnight. And even the very best couples currently have moments of resentment or anger.
So how can you spot these times and steer clear of them via sabotaging your relationship?
The easiest way is to seek out help early on. That’s because half of all of the marriages that end accomplish that in the initial seven years, and if you’re unhappy together with your partner or perhaps marriage for a little bit, it’s a chance to talk.
A happy marriage depends on attention. A romance built to last starts with a dedication to staying away from harsh and negative communications, like criticism, contempt, defensiveness and stonewalling Asian Woman And Mail-Order brides (withdrawing and turning down).
In fact , explore shows that a five-to-one ratio of positive to negative communications is a strong predictor of success in a marriage. Which means, for every snide comment or outburst, there ought to be five great interactions, for example a kiss, an endearing smile, a enhance or an intentional second of playing hear the additional person’s perspective.
That ratio may not could be seen as a lot, nevertheless it’s actually quite high for the purpose of married those who have been together for a long time and happen to be in good health. And it’s a ratio https://www.cyber-lynk.com/blog/2020/07/27/benefits-associated-with-dating-philippine-females-on-line/ that’s susceptible to drop in a short amount of time, that is why it’s so critical to ramp up the amount of positives.
It also signifies that each partner has to make an attempt to do their part. It means apologizing to get the aggravating words or perhaps actions, currently taking responsibility and making compensation when practical.
Lastly, it implies that each partner has to accept their faults and focus on them along. That might suggest a change in behavior or a different ways to problem-solving.
But you may be wondering what makes it most worthwhile is that couples exactly who work on their weaknesses in the beginning of matrimony are far not as likely to obtain conflicts and issues that will derail the relationship down the line, and even trigger divorce. So if you may get your partner to understand that their flaws is surely an inevitable part of simply being human, it’ll be much easier to progress together and solve gross conflicts.


text:
Gov. Jim Hodges and state law enforcement leaders began
pre-paring Tuesday to stop federal plutonium shipments headed for
South Carolina next month from a nuclear weapons complex in
The governor's preparations come amid escalating tensions between
the Republican Bush administration and Democrat Hodges, who opposes
plutonium shipments to the state without a legally binding agreement
that it won't be stored here forever.
Plutonium is a highly toxic material used for decades to make
Hodges didn't provide many details of how the state government
would stop federal trucks laden with plutonium destined for storage
at the Savannah River Site. But, in addition to a possible lawsuit,
Hodges hasn't ruled out using state troopers and transport officers
to delay or block the plutonium convoy.
During a brief meeting open to the news media, the governor told
the state Department of Public Safety to examine "safety concerns"
about toxic shipments on state highways and report back to him next
He also ordered the department to "thoroughly examine" the
entrances to the SRS site. He wasn't more specific, but his concerns
likely center on routes that trucks might take to reach the nuclear
weapons complex outside Aiken near the Georgia border.
Additionally, Hodges said S.C. transportation officials need to
talk with their counterparts from neighboring states about shared
concerns over the plutonium shipments. Hodges said officials in
other states would need to be aware of South Carolina's plans if it
tried to stop plutonium shipments at the border.
Hodges' orders will update plans his office made last summer.
That's when South Carolina learned plutonium could be shipped to SRS
without a clear plan to remove it, Hodges has said.
"Our goal is to make sure that no plutonium is shipped into South
Carolina's borders without a firm agreement on how the material is
going to be treated and when it is going to leave," Hodges said. "We
need to look at every-thing that's available to help us in achieving
our goal of protecting the public health and safety of South
Federal Energy Department spokesman Joe Davis criticized Hodges'
actions Tuesday, saying the federal government wants a compromise
with South Carolina. Energy Secretary Spencer Abraham has offered
plans to Hodges that Abraham says would ensure the plutonium doesn't
stay in South Carolina indefinitely.
The DOE formally notified Hodges late Monday that it would start
shipping plutonium to SRS as early as May 15. The energy department
still hopes to work out a compromise with the governor that
satisfies his concerns, Davis said.
"I think it's astonishing ... that the governor would choose
to have a meeting to try to build a better roadblock, rather than
work with us and the members of Congress," Davis said. "This is
Trucks full of plutonium from the Rocky Flats, Colo., site would
be guarded by federal agents, officials have said. The plutonium
would come in tightly packed, double-insulated containers for
shipment to SRS, Davis said.
The office of U.S. Sen. Strom Thurmond, R-S.C., said Tuesday the
senator was working to get the plan added to the fiscal 2003 defense
bill. That would ensure the plutonium would leave SRS eventually,
his office said.
The dispute has simmered for months over federal plans to process
plutonium at SRS into fuel for commercial nuclear power plants. The
excess plutonium would come from old nuclear weapons sites across
the country that are being cleaned up.
Arms agreements with Russia call for both countries to neutralize
weapons-grade plutonium so it can't again be used for atomic bombs.
One way to do that is through the mixed oxide fuel, or MOX, plants
to be built at SRS.
Hodges, however, says there's no guarantee the MOX plants will be
built and South Carolina would be stuck with the plutonium. The Bush
administration has pledged nearly $4 billion over 20 years to build
the plants. But Hodges argues Bush can't say what will happen in 20
In the meantime, Abraham has offered to move the material out of
the state if the plutonium program goes awry. Hodges wants a federal
court order to make sure that happens, but Abraham has balked at the
Tuesday's developments came as state Republican leaders
criticized Hodges for failing to accept the Bush administration's
offer to resolve the dispute.
"What I'm puzzled about is the governor appears determined to
have a confrontation," said Attorney General Charlie Condon, a GOP
gubernatorial candidate who spoke Tuesday with Abraham. "The energy
secretary says he will do anything to let South Carolina have a
legally binding and legally enforceable agreement."
Lt. Gov. Bob Peeler, also a GOP candidate for governor, and
Republican House Speaker David Wilkins issued a joint statement
accusing Hodges of political posturing.
"The transportation of nuclear material is serious business done
by serious people," the statement said. "Holding irresponsible
posturing meetings for the public is not helpful."

domain: news


The August Smart Lock provides intelligent, secure access to your home. Now you can control who can enter and who can’t—without the need for keys or codes. And you can do it all from your smartphone.
August Smart Lock’s encrypted technology is safer than keys that can get lost or codes that can be copied. You can still carry a key. You just don’t have to. August Smart Lock is powered by four AA batteries so it’s always on – even if the power, Wi-Fi, or cable go down.
When you return home, August Smart Lock senses your approach and automatically unlocks your door. There’s no need to reach for keys. August Smart Lock can also automatically lock your door when you leave, so rest assured your door is locked.
Check the video below:


text:
Admission by donation
The War Between Us takes place during WWII in the interior of BC and deals with the effect of the Canadian government’s policy of internment on the West Coast Nikkei community. Nominated for 6 Gemini Awards, and was the highest rated film broadcast on Canadian TV in 1995. Directed by Anne Wheeler, starring Shannon Lason, Mieko Ouchi. 1995. 93 minutes.

domain: library


text:
Sixmile Leisure Centre is the first facility in Ireland to get the new Easyline Circuit from its current fitness equipment partner Technogym.
The Easyline circuit has been developed to provide a good training solution to those people who find gyms intimidating. It is a perfect quick training solution for strength and metabolic training and customers will soon feel that training is the most natural thing in the world.
It has a user friendly design and training with Easyline is relaxing and enjoyable. The push and pull push exercises are carried out on hydraulic resistance rather than metal plates, the harder you push or pull the more resistance you achieve. It provides a complete and satisfying workout and you can step into any piece of equipment while doing a circuit.
Easyline Circuits Class
Why not join our total body workout?
This is a circuit class including step, dumbbells and resistance equipment.
- 6.00pm – 7.00pm (Vicki Carson)
- 7.00pm - 8.00pm
Book at Reception (limited spaces).

domain: health


text:
Photo by Daniel Guillemette.
Every day countless crack-cocaine users across Vancouver put themselves at risk of disease by sharing crack pipes or using makeshift devices such as bottles, cans or cheap metals. While the federal Conservative government would disagree, Vancouver Coastal Health’s recent decision to hand out free, clean crack pipes will help some of our most stigmatized drug addicts stay alive.
Following the lead of cities such as Calgary and Winnipeg, Vancouver Coastal Health (VCH) will start handing out the pipes later this year. Since statistics show that most addicts share pipes, and diseases like HIV and Hepatitis C are transmitted through the open sores on cracked lips, the clean pipes will help cut down on infectious disease rates. It is a smart policy that will save both lives and money.
Unsurprisingly, it didn’t take long for the federal government to display its continued ignorance on the subject of drug addiction by opposing the project. “I am not in the business of passing out free crack pipes to anyone,” federal Justice Minister Rob Nicholson was quoted as saying. “Our emphasis has been to get people off drugs.” For a government that has fought tooth-and-nail to shut down Vancouver’s safe-injection site, despite overwhelming science supporting it, what else could one really expect?
In addition to the federal government’s ideological battle, there are many others who refuse to hand a lifeline to crack addicts. Unlike heroin users, who enjoy at least some cool caché from modern pop culture, there is little sympathy from society for crack-cocaine. Even on the streets, “crack heads” are often seen as the lowest of the low—even by many crack users themselves.
Without the immediate danger of overdose, addicts of this incredibly destabilizing drug have been left to their own devices. That’s meant sharing pipes and, sadly, diseases.
It’s time that crack addition be taken more seriously in our city. Most people are unaware that when the safe-injection site on East Hastings was created, they built a “safe-inhalation room” for crack smokers.
Of course, with little hope for federal approval, it’s never been used. That is a waste and it means more chaos for the users and for our streets. Megaphone staff can certainly attest to the fact that the drug long ago overtook heroin as the most popular in the Downtown Eastside—every day we have to politely remind people that our doorway is not a smoking den. It's clear: what crack addicts need is a safe place to smoke and clean pipes to smoke with.
Hopefully the VCH’s project will be the start of a new wave in how to deal with crack-cocaine addiction in Vancouver. We need to find ways to keep crack users healthy before we can begin to address how we can get them clean.


Online Shop Prices(New Mobile)
4 GB RAM Red
- Xiaomi Redmi 5 plus is manufactured by Xiaomi mobiles.
- Xiaomi has launched almost 25 mobiles till today in Pakistan.
- Xiaomi is launching almost 40 mobiles very soon in Pakistan.
- Xiaomi Redmi 5 plus is available in Black, Red, and Gold colors in Pakistan.
- This mobile launched with RAM of 4 GB, and 3 GB in Pakistan.
- This mobile launched with Storage of and 32 GB in Pakistan.
Excellent specifications means you can trust on Xiaomi Redmi 5 plus mobile
Ranking higher among peoples who are loving it
Higher group ranking means this mobile is taking more people interest in thier price range other mobiles
Internet surfing, Reading and watching videos is more perfect on a bigger screen.
There will be Normal sharp and clear videos, Images and text
Perfect screen size with perfect resolution will gives you excellent experience
Superb option for back camera selfie for videos & images
Normal camera to take normal photos & vidoes
Sppeed of mobile will be high due to high RAM & Processor
Faster CPU means more fast processing speed & experience.
Long battery time to talk, read & watch videos.
Bad option for front camera selfie for videos & images
Xiaomi Redmi 5 plus Description
Xiaomi Redmi 5 plus or also known as Redmi Note 5 is launching with the plus version of its predecessor smartphone, to stand among the famous brands this smart phone is coming with the full vision display like other well-known brands. Unlike its predecessor it is stunning in design and it is predicted that it will be launched before the note series of same brand.
This smart phone is offering you the resolution of 720 x 1280 along with the display screen of 5 inches. This brand indicate chat Chinese are not going with the traditional design of smart phone, as the revealed design states that, the upper and lower portion of the bezel are having front facing camera and speakers. To sharpen the image quality of the phone, dual camera is installed in the device. This device is going to offer you two variants with 4 GB and 3 GB Ram.
Price has increased 14.29% from Rs.17999 on 21 February, 2018 to Rs.21000 today.
Xiaomi Redmi 5 plus Review
Xiaomi Redmi 5 Plus Full Review | One Weak point!!!
Xiaomi Redmi 5 Plus Unboxing & Hands-On Review
|OS||Android v7.1.2 Nougat|
|Dimensions||158.5 x 75.5 x 8.1 mm|
|SIM||Dual Sim, Dual Standby (Nano SIM)|
|Colors||Black, Gold, Light Blue, Rose Gold|
|2G Band||SIM1: GSM 850 / 900 / 1800 / 1900 |
SIM2: GSM 850 / 900 / 1800 / 1900
|3G Band||HSDPA 850 / 900 / 1900 / 2100|
|4G Band||LTE band 1(2100), 3(1800), 5(850), 7(2600), 8(900), 34(2000), 38(2600), 39(1900), 40(2300), 41(2500)|
|CPU||2 Ghz Octa Core Cortex A53|
|Chipset||Qualcomm MSM8953 Snapdragon 625|
|Technology||IPS LCD Capacitive Touchscreen, 16M Colors, Multitouch|
|Resolution||1080 x 2160 Pixels (~403 PPI)|
|Protection||Corning Gorilla Galss (unspecified version)|
|Built in||32 Built in, 3 RAM|
|Card||microSD Card, (supports upto 256GB) (uses SIM 2 slot)|
|Main||12 MP (f/2.2, 1.25 Î¼m), autofocus, dual LED dual tone flash|
|Features||Phase detection, Geo tagging, touch focus, face/smile detection, HDR, panorama, Video ([email protected])|
|Front||5 MP, 1080p|
|WLAN||Wi Fi 802.11 a/b/g/n, Wi Fi Direct, hotspot|
|Bluetooth||v4.2 with A2DP, LE|
|GPS||Yes A GPS support, and GLONASS, BDS|
|Data||GPRS, Edge, 3G HSPA, 4G LTE|
|Sensors||Accelerometer, Compass, Fingerprint (rear mounted), Gyro, Proximity|
|Audio||3.5mm Audio Jack, DivX/Xvid/MP4/H.265 player, MP3/WAV/eAAC /FLAC player, Speaker Phone|
|Messaging||SMS(threaded view), MMS, Email, Push Mail, IM|
|Games||Built in Downloadable|
|Extra||Active noise cancellation with dedicated mic, Photo/video editor, Document viewer|
|Capacity||4000 mAh (Li Po, Non removable)|
|Capacity||Fast battery charging 5V/2A|
|Price||Price in Rs: 21,000 Price in USD: $182|
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[pmwiki-users] Permissions in IIS6
Dave.Jackson at emrltd.com
Thu Sep 11 10:58:41 CDT 2008
I have been mucking about on my 'test' IIS server today, which
incidentally hosts our internal wiki. I added Frontpage Server 2002
extensions and added basic authentication to try and get Visual Studio
2008 to 'talk', specifically, publish to it. Went like a charm but then
I found I no longer had write access when I tried to save a page on the
wiki. First it complained about the .flock file, which I then deleted
and it got past that, but now it says it cannot write the file in the
wiki.d folder that is the page I am trying to edit.
So I uninstalled front page extensions, turned off basic authentication
but it still doesn't work. Even after turning it off & turning it on
Any thoughts on what else I can try before I go back to backup?
****** This email is sent for and on behalf of European Metal Recycling Limited ******
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More information about the pmwiki-users


text:
Trimpley Sailing Club is a small, friendly, family focussed club in the Severn Valley, North Worcestershire.
We are a RYA approved training centre. If you’ve never sailed before we offer learn to sail and power boat courses throughout the year. If you’ve done some sailing before we can help you improve; we have club racing all year round, and training courses to help you get better.
There is a great social side to the club. We have evening socials through the year and organise away trips too.

domain: sports


Magnetic DNA purification is a simple and reliable way to isolate DNA.
This method for purifying DNA has a wide range of applications. In fact, genomic sequencing and molecular analysis have become so integral to biological research that they are now all but required for work to be published in high profile journals.
Outside of scientific research, magnetic DNA purification is also fundamental to forensic analysis in the criminal justice system. This requires the ability to rapidly extract and purify high-quality DNA and RNA from a variety of tissues. As a result of this demand, improvements to current techniques are needed.
History of DNA purification
Our understanding of genetic material has increased substantially since Friederich Miescher performed the first DNA extraction in 1869. That first extraction led to the simple discovery that a material exists within cells that precipitates out of acidic solution and dissolves into alkaline solution. After that, it took until 1953 for the full structure of DNA to be uncovered. Shortly after this, standard laboratory procedures for isolating nucleic acids emerged. But what are the modern methods used for DNA purification?
How does magnetic DNA purification using nucleic acid isolation work?
Nucleic acid isolation and purification consists of three basic steps:
- Disruption (or lysis) of cells
- Removal of protein and contaminants
- Recovery of DNA
The disruption of the cells is a mechanical process: tissue is typically frozen in liquid nitrogen and crushed to break the macroscopic structure and to rupture cell walls. This slurry of tissue is then mixed with solvents and salts to wash out unwanted proteins, disable DNAses, and collect the DNA into a usable solution. Finally, a phenol-chloroform organic solvent is used to concentrate DNA in a hydrophilic phase. This hydrophilic solution can then be collected, centrifuged, and washed until pure DNA is recovered.
Earlier methods involved the DNA collecting in the pellet at the bottom of the tube, which would then be dissolved in water and collected. To increase yield and streamline protocols between laboratories, solid phase support systems were developed. Currently, commercial kits using a combination of solvents and solid-phase support columns are widely used to isolate and purify DNA and RNA.
What is column-based DNA isolation?
This method uses proprietary columns, sold commercially in isolation kits, that are made of silica matrices, glass particles, diatomaceous earth, or anion-exchange resins. The DNA or RNA binds to the column while proteins and contaminants are washed out by ethanol using a centrifuge. This process is repeated, and finally the remaining nucleic acids are collected with water.
Due to the widespread availability and affordability of these commercial kits, DNA isolation is relatively easy, quick, and standardized. However, the process still requires many buffers, solvents, expensive centrifuges, laboratory space, and time to complete.
The need to increase the efficiency of this process led to the development of magnetic DNA purification methods. The advances in nanotechnology over the past 20 years have made magnetic bead DNA extraction a viable and increasingly attractive method of nucleic acid purification. Magnetic DNA purification doesn’t require columns or centrifugation, and enables the process to be automated.
How does magnetic bead DNA extraction work?
Magnetic beads are a collection of uniform particles, 500 nanometers by 500 micrometers in diameter, which are made with magnetic materials. These magnetic materials are typically composites containing nanoparticles of magnetite. The particles become magnetized in the presence of an external magnet, making them easy to manipulate.
The United States patent entitled ‘DNA purification and isolation using magnetic particles’, was published in 1998. This early magnetic separation technique used particles consisting of an iron-oxide core coated with silane. The surface of the particles was then bound to molecules containing a free carboxylic acid, which in turn bound to DNA or RNA. The salt concentration determined the strength of the bonds between functional groups and the nucleic acids, which allowed for controlled reversible binding. At the correct salt concentration, the nucleic acids would bind to the magnetic particles. Next, a magnet was placed outside of the tube containing the solution to create a strong external magnetic field. The magnetic particles bound with nucleic acids were attracted to the field and “stuck” to the outer edges of the tube.
An alternative technique used a magnet at the bottom of the tube to draw particles downward. In both cases, the magnetic field is maintained while the unwanted solution is exchanged and contaminating proteins are washed away. After the washing steps, the DNA or RNA is released from the magnetic particles with an elution buffer, leaving behind a pure sample ready for quantification and analysis.
How are functionalized magnetic beads used for nucleotide extraction?
In recent years, modifications to the patented magnetic DNA purification method have been developed. The magnetic particles can now be made from synthetic polymers embedded with iron oxide ‘pigments’, or metallic cores of iron-oxide coated with a polymer surface to enable functionalization. These uniform particles can be coated with functional moieties, or can be left uncoated.
Some functional coatings work via electrostatic interactions (positively charged amine or imidazole moieties), while others work via salt- or pH-mediated attractions (silica and carboxyl groups). More specific DNA (or RNA) isolation is enabled by the introduction of target oligonucleotides to the magnetic bead surface. These target oligonucleotides are useful for the extraction of specific sequences of ssDNA or RNA.
Magnetic DNA purification is a clear improvement on centrifuge-dependent isolation techniques when using semi-automatic or fully automatic systems. These systems are used when rapid purification of many samples is necessary.
In the absence of magnetic DNA isolation, the need to move the samples into and out of a centrifuge is inefficient, and can increase analysis time. In contrast, the use of magnetic particles allows the tubes to remain in one machine throughout the entire process.
What are some examples of DNA isolation by magnetic beads?
Magnetic beads are used to purify DNA in a wide range of different situations. These include:
Plasmid DNA isolation
Magnetic beads are used to isolate plasmid DNA from crude extract. This is possible through carefully optimized solutions that separate plasmid DNA from genomic DNA and proteins, before allowing the plasmid DNA to bind the magnetic beads and introducing them to a magnetic field.
Genomic DNA isolation
Magnetic beads are also used to separate genomic DNA from proteins and RNA from crude extract. The optimization of salt, pH and charge in solution means only the genomic DNA can bind to the beads, which can then be placed in a magnetic field for separation.
DNA fragment isolationThere are kits available for many types of DNA isolation, including for DNA fragments. DNA fragments may need to be isolated for next generation sequencing protocols, where the fragments can be separated using beads that can select for the size of a molecule.
Magnetic beads makes DNA purification simple
The ease-of-use and scalability of magnetic DNA purification techniques will be increasingly useful as scientists dig ever deeper to untangle the complicated molecular bases of observable phenotypes. Genetic analysis and manipulation is the way of the future, and using magnets to isolate pure DNA and RNA will help to accelerate the pace of discovery.
- DNA purification and isolation using magnetic particles. A Hawkins, T. http://www.google.com/patents/US5705628. 1998. Google Patents.
- Tan SC, Yiap BC. DNA, RNA, and Protein Extraction: The Past and The Present. Journal of Biomedicine and Biotechnology 2009;2009:574398. doi:10.1155/2009/574398.
3. Z.M. Saiyed, C. Bochiwal, H. Gorasia, S.D. Telang, C.N. Ramchand,
Application of magnetic particles (Fe3O4) for isolation of genomic DNA from mammalian cells, Analytical Biochemistry, Volume 356, Issue 2, 15 September 2006, Pages 306-308.
- Targeted Magnetic Microsphere Delivery of DNA to Encourage Vascular Endothelial Growth
- Controlling Mesenchymal Stem Cell Growth with Magnets in Lieu of a Scaffold
- Magnetic Particles in Fluorescence Activated Cell Sorting


Description: comes with 4 pheromones to be used in the Buxatrap for monitoring and trapping male box tree moths.
Best Used: from May/June until the end of November (dependent on geographic location) and in conjunction with the box tree moth trap (Buxatrap) and Capsanem.
Works By: dispensers releasing a species-specific sex pheromone, which lures the males of box tree moths into the trap. Once in the trap, they cannot escape and are easy to detect, count and identify. Trapping males also aids in reducing the number of eggs laid by females, as they fail to mate.
Suggested Application Rate: 2 Pherodis pheromones (Cydalima perspectalis) per 1 trap placed every 30-180m2, depending on infestation pressure.
Packaging: comes with 4 pheromones.
Preparation: For each trap, two Pherodis dispensers need to be used. Place the Pherodis pheromone dispensers in the canisters. Assemble the trap and fix the canisters to the trap. Place the trap on a stick, at a height of 1.5M. Replace the pheromone every 8wks. A "How To Use" video can be found in the link below.
For More Information, Buxatrap | Koppert Products


The CAA is investigating a website called Click2Flight offering flights and holidays for sale in the UK.
The investigation follows a call to Travel Weekly from Avon and Somerset police after a consumer reported booking a flight which did not exist.
Click2Flight offers “cheap flights”, including with British Airways and Virgin Atlantic, primarily to long-haul destinations.
Its site carries an Atol logo, although the number is illegible, and claims: “We are fully bonded of ATOL, UK APTA, IATA.”
The company suggests APTA stands for Asia Pacific Travel Partner. A spokeswoman for the Pacific Asia Travel Association (PATA International) said she had never heard of the group.
Click2Flight claim to act “as agents”, stating: “The Company are not the Principal and do not act as the Principal.” It appears to offer hotels and car hire but clicking on these options merely returns a user to the flight options.
The CAA confirmed the company does not hold an Atol and UK Companies House shows no record of the firm.
Click2Flight provides a London address and phone number but had not responded to email or phone messages. The address in south London appears to be occupied by a hardware shop.
A CAA spokesman said: “There are a few things we have concerns about and we’re looking into it. They don’t have an Atol. You can’t see the Atol number. It’s not clear who they are an agent of.”
The company claims to have won a 2012 Readers Choice Award from the US Travel Weekly, but the Travel Weekly site shows it was not among the winners.


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Peek-A-Boo I See You!
My oldest son, somewhere in the process of digging our snow cave. Gotta love having this much snow at the end of March! Let's see, at this rate we should be able to get outside and climb by August or so... I gotta take up Ice Climbing!
|Photo Location:||No Location Information|


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CHRIS MUTOMBO / EUGENE KHONDE (https://laprosperiteonline.net/)
Link to the original article in French
Facilitation of international maritime traffic: OGEFREM pleads for the ratification of the Fal Convention
From right to left, William Kazumba, CEO of Ogefrem, Marc Ekila Deputy Minister of Transport and the Representative of the SG of the IMO
Under the impetus of the International Maritime Organization, IMO in acronym, the Multimodal Freight Management Office, led by its Director General William Kazumba Mayombo, is in a capacity building seminar on the facilitation of international maritime traffic Convention Fal .
Opened this Wednesday, October 5, 2022 at Beatrice Hotel, by the Vice-Minister of Transport, Communication Channels and Accessibility, Mr. Marc Ekila Likombo, representing the Minister in charge Chérubin Okende prevented, this international seminar which will last three days, from October 5 to 7, 2022, takes into account the participation of international experts and operators of the maritime sector, IMO consultants, experts and General Managers of companies under maritime transport supervision.
First to speak, the representative of the Secretary General of the IMO gave the quintessence of these foundations in the field of international maritime trade.
Given the imperative and irremediable nature required by the use of the Fal Convention, the CEO of the Multimodal Freight Management Office, William Kazumba, requests the support of the Government of the Republic through technical supervision for the ratification of this False Convention. This will allow Ogefrem to fully play its role as a Council of shippers and shippers to benefit from all the advantages attached to it like other African countries, specifies the boss of Ogefrem. Because, he added, it is in the interest of the DRC, a landlocked country whose economy is essentially extroverted, to engage in it.
A view of the seminar participants
Previously, William Kazumba, DG of Ogefrem placed his words well to thank with deep respect, the Head of State, Félix Antoine Tshisekedi Tshilombo, for having placed his trust in their modest person in order to preside over the destiny of this big machine that is Ogefrem. His thanks also went straight to the Prime Minister, Head of Government Sama Lukonde and the Minister in charge Chérubin Okende for his personal involvement in the materialization of this seminar based on the facilitation of international maritime traffic.
Entering into force in 1967, this convention aims to simplify formalities, documentary requirements and procedures for the transport of goods from the country of embarkation or origin to the country of destination or country of arrival.
According to DG Kazumba, statistics show that countries with an infrastructure of the Fal Convention have better turnover figures for both imports and exports.
For that reason, he appreciated IMO's technical assistance.
Thus, the said seminar will effectively equip the authorities, executives and agents of the maritime sector on the procedures for setting up the single window and the advantages linked to its ratification for the Congolese maritime industry.
But beyond that, the DG says he is confident that thanks to the participation of IMO experts and consultants in this seminar, the various stakeholders: government, parliament and operators in the sector, civil society, etc., will ask the acts that are necessary in order to boost the field of foreign trade thanks to the mechanism of the Fal Convention.
Resolutely committed to the mission of facilitation and assistance in favor of importers and exporters of the DRC in their daily activities and of all stakeholders in the sector, it goes without saying that Ogefrem emphasizes the infrastructure of support for the activities of shippers and end-to-end Congolese freight management instruments, that is to say, from the country of origin, producer to the destination, country of arrival.
A listening ear
For his part, the Deputy Minister of Transport, Ways of Communication and Accessibility, Mr. Marc Ekila, first paid tribute to the President of the Republic Félix Antoine Tshisekedi Tshilombo, for his vision based on "people first and which gives the impetus to commit to revitalizing the maritime transport sector on the path of development and to make it a pillar if not a real engine of economic growth.
Then, to the Prime Minister Sama Lukonde and the Minister of TRANSVCOM Chérubin Okende by equipping the Authorities, executives, agents and public and private companies of the maritime sector, the process of setting up the Single Window on the Fal Convention for the facilitation of international maritime traffic.
Against all odds, he called on the delegates of companies and administrations of the Congolese maritime sector to pay sustained attention to this seminar during which the substantive questions on the general articulation of the Fal 45 convention and its applicability in DR-Congo, IMO member country.
To do this, he invites all experts to seize this opportunity to analyze the operation and current architecture of the DRC's one-stop shop in order to propose improvements if necessary and complete, in the best interests of the Republic, the process of ratification.
Family photo with the Experts and Consultants of the Maritime sector
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Perfect for fans of Christina Lauren and Emma Chase—the second novel in New York Times bestselling author Monica Murphy’s sizzling series about three powerhouse sisters and the men who would have their hearts.
People say the youngest child has it easy, but nothing can be further from the truth. Unlike my two sisters, Violet and Lily, I’m never in the limelight. I just work my butt off for Fleur Cosmetics and get little to no thanks for it. I’ve been pushed too far one too many times, and I’m finally brave enough to do something about it.
Maybe my newfound courage has something to do with the amazing pink and white diamond necklace I wear to the party in Cannes. The instant those dazzling heirloom jewels touch my skin, they excite some deep, aching need inside. And when that guy—that totally gorgeous guy—locks eyes with me, I know this nice girl is going to be naughty.
For once it’s my turn. My turn to say no to my father, to outshine my sisters, to walk away from it all—straight into the arms of a mysterious stranger. But what if Caden is much more than I bargained for? Sure, he makes me feel sexy and free in a way I never have before, but there’s something else I can’t quite place—something dangerous. Maybe our “chance” meeting wasn’t so random. Maybe he was looking for me for a reason. Whatever his motive, there’s no going back now.
Watch the Trailer
Read an Excerpt HERE
*This was an ARC provided to me for free in exchange for an honest review. All thoughts and comments are my own.*
Stealing Rose is the second book in The Fowler Sisters series by Monica Murphy and follows the youngest Fowler sister Rose. I’ll be honest with you, when I first attempted to read Owning Violet I couldn’t get into it. I tried on three different occasions and each time I ended up putting the book down. I don’t know why because the story isn’t bad, the writing is engaging and steamy as hell. I just for some reason couldn’t get into it. Finally, one day I sat down opened it up and I swear it was like I picked up a different book and I completely devoured it. Maybe I wasn’t in the mood…I still have no clue what it was, but in the end I did enjoy the first book in the series which made me continue with the series. Stealing Rose is incredibly hot! Boy did I have to fan myself a few times. Not only does this story deliver Rose’s romantic struggles, but also keeps up a bit with areas of Violet’s life (allowing the reader to keep up with some of their favorite characters). Monica Murphy continues the series in dual POV which I absolutely adore.
I loved everything about Stealing Rose. The chemistry between Caden and Rose made my Kindle spark! Talk about steamy! I’m still blushing and fanning myself. I definitely enjoyed Rose’s story a bit more than Owning Violet, but I have officially fell in love with the series after this read. Caden meets Rose in Cannes and there is an immediate attraction between the two. One that is undeniable. Rose is going through a reflective stage, she’s trying to figure out her place in the Fowler family and within their family’s empire, Fleur Cosmetics. She feels underappreciated and due to recent developments her relationship with her father has turned sour. Enter Caden, a cup of tall, dark and handsome. He brings out the absolute best and worst in Rose. He pushes her buttons and drives her crazy in so many ways, but she can’t help the pull she feels to him. He makes Rose feel alive and frisky. She’s having fun and learning about herself in the process, but how much does she really know about him? Will his secret tear them apart or bring them closer together?
I couldn’t put this one down. What drew me into this series is how real these “heiress” characters feel. They’re wealthy, but they work their butts off to keep their family business running. They aren’t arrogant or greedy and Monica Murphy has made them “human.” You connect with them without feeling like they’re these privileged princesses. You can see that they all care for each other a great deal in their own ways. Violet annoyed me a bit in this book she was a bit judgmental and a bit of a hypocrite, but I understood and liked that at one point she’s called out on it. It’s actually a breath of fresh air to read about three sisters like Rose, Violet and Lily. After reading Stealing Rose, I cannot wait for Lily’s story. You are in for a treat with an ending that will tear you up in the best possible way.
I highly recommend this read to Erotica/Erotic Romance fans.
Happy Reading Lovelies xo
The Fowler Sisters Series:
*Add this Series to your Goodreads
About the Author
Monica Murphy is the New York Times bestselling author of One Week Girlfriend, Second Chance Boyfriend, Three Broken Promises, Four Years Later, Owning Violet, Stealing Rose, and the eBook novella Drew + Fable Forever. A native Californian, she lives in the foothills of Yosemite with her husband and three children.
Connect with Monica

domain: entertainment


The lads from Limp Bizkit are setting up quite a busy summer for themselves.
Not only is the band slated to appear at Woodstock '99; it'll also be dropping its second album, "Significant Other," on June 22, after which it will hit the road for not one, but two separate tours.
Limp will tour in tandem with Kid Rock and Staind before going out later this summer for its virgin stint as headliner of the 1999 Family Values Tour (while festival founder Korn is sequestered in a studio, recording another album).
According to frontman Fred Durst, the recording of "Significant Other" gave him the chance to approach one of his rap heroes: Method Man, who guests on the track "Shut the F*** Up."
"[A] straight hip-hop track with me and Meth, I think, is what people want," Durst told MTV News. "I think if Method Man came on our album to jam, they wouldn't want us to do some crazy-rocky-eclectic-artsy
song to see what Meth and Limp could create that's crazy. What people want to hear is that 'I came to bring the pain' thing... and 'They call me Big John Stud/My middle name Mud.' I mean, that's what you want to hear, and that's what you got." [RealVideo]
In addition to Meth, the upcoming record boasts guest vocals from Eminem on "Turn It Loose," and the track "Nobody Like Me" features Korn's Jonathan Davis and resurrected Stone Temple Pilot Scott Weiland.
As for this year's Family Values, no other acts have yet been confirmed (see "Limp Bizkit Leading Family Values, But Who Will Join Them?"), but we'll keep you posted.


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Could only pay cash in the plane for drinks. I flew from Thailand to indonesia so i did not have rupia yet. Credit card payment was not possible
飞机 经济舱, Lion Air, 2019年11月7日
Although it was written on the ticket that 1piece of baggage with max. 20kg is for free, the checkin insisted that I have to pay 20euros for my bag. Why: the ticket was bought through an agency (12asia) and not at lion directly. Poor communication?

domain: travel


Show GenresSoul Industrial Drum & Bass Punk News Classical Rock track reviews Funk Space Bass Psychedelic FunkRock Electronic electro funk rocknrolla kartell Talk Show Pop Jazz Blues happyoblivion Hawaiian Folk Classic Rock World sankai hineri Deep House Indie Rock Electro-rock-pop-hop 2013 Dub Reggae Reggae Multi-Genre japan Dancehall Latin Dubstep indie pop Soundtrack Bass Hip-Hop
The KTUH Alumni Show
Voices from the past return to the airwaves every Saturday afternoon. Each week, a different KTUH alum returns to lay down three hours of grooves, just like old times. We never know what will be played from one week to the next, so tune in and find out for yourself!
If you’re a KTUH alumnus or alumna and would like to host an Alumni Show, contact our Alumni Director at firstname.lastname@example.org for more information.


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Midwesterners are feeling the squeeze as the record drought continues. They’re forced to choose between potentially loosing tens of thousands of dollars of turf and landscapes and increasing water usage to keep their landscapes and lawns alive. This is a no-win scenario.
You may be asking why your water bill is so high or why your plants are declining. If so, you’re not alone. We’re all asking those questions.
We’re in the middle of what weather experts are calling the most severe drought in the last 89 years. This multi-month drought has sapped the moisture out of our soils and is not predicted to get better in the next few months.
The United States Drought Monitor site has mapped the Kansas City region as falling into the “Extreme to Exceptional” category.
(See source: https://droughtmonitor.unl.edu/ConditionsOutlooks/CurrentConditions.aspx)
How Are Our Landscapes Affected in Kansas City?
Plant health and vitality are directly linked to the environment they’re living in. The drought combined with a pattern of extreme heat in summer and extreme cold spells in
winter has caused abnormal stresses on both new and old plants. If you have evergreens, the stress is more significant.
(See post here explaining the science: https://www.instagram.com/p/CjRB91lpVmF/?utm_source=ig_web_copy_link )
Watering is the primary way to attempt to overcome these environmental stressors. Even with watering appropriately, the stressors can be too much.
We’re receiving calls from people who don’t have irrigation plans who have lost their lawns and landscapes from not watering enough. We’re also receiving calls from people who have saved their landscapes with irrigation plans who are upset because they’re using extra water. Nobody is winning in this scenario. You either save your landscape and lawn with extra water use, or you risk losing it and having to replace it all. That’s the harsh reality of extreme drought conditions.
What Can You Do?
· If your irrigation provider has been making suggestions about needed repairs, clock replacements, or improvements, now may be the time to ensure your system is as
efficient as you can get it by making those improvements.
· Proactively plan to have your evergreens and plants less than five years old hand-watered this winter if you have your irrigation system winterized. This may be an extra
investment, but will help protect your landscape investment. Please let your account manager know if you’d like to have a watering plan for winter.
· Determine if the value of your turf and landscape is worth more than the water bills. If you determine you’d rather turn down the water to less than horticulturally needed, you have the authority to make that decision. Just understand the consequences of that decision may result in significant loss.
· Consider other ways to conserve water or reuse water. Rain barrels, cisterns, drainage swale systems, rain gardens, permeable patios/drives, and many other types
of projects can help mitigate these challenges in the future.
Recovery from drought conditions is not a quick process. It requires deep, saturating moisture over time to get the soil conditions back to normal. This means that we may
have several months if not into 2024 before we begin recovering. This means having conversations with your care providers about your priorities and expectations is
· Decide how you’d like to address moisture in wintertime when your irrigation system is winterized.
· Sit down with any decision makers in your home to prioritize how you’d like to address water usage versus plant loss risk.
· Communicate with your account manager or care provider if you have questions or concerns.
· Consider making those irrigation system improvements. Saving water is incredibly important right now.
As we’re all struggling to keep plants and lawns alive, we think about the farmers and ranchers who are struggling. Their battle to overcome these conditions should not be
Many states are beginning to implement policies limiting water use for lawn/landscape purposes to ensure people, animals, and farms have access to water. While this hasn’t happened in our region at this time, we must understand that at some point, your irrigation choices may become limited. Some municipalities are raising water prices to passively discourage water use. We encourage you to monitor your water for all parts of your property during this time.
(Photo Source: United States Drought Monitor)

domain: agronomy


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The flapper roll call from Josephine Baker to Dorothy Parker and Edna St. Vincent Millay is incomplete without Zelda Fitzgerald. Hard-drinking sybarite relentlessly on the move to be wherever the party was, an artist and amateur ballet dancer, a journalist and novelist con brio, the fashion-forward Zelda was a meal ticket for gossip columnists from the time she hit Manhattan.
Biographers batten on her dalliances and feuds (notably, with Ernest Hemingway), even as literary critics call for greater recognition for her novel Save Me the Waltz. Join cultural historian Charles A. Riley II for an insider's look into the creative life of a Jazz Age legend, including the secret role she played in the revision of The Great Gatsby.
“Churchwell… has written an excellent book… she’s earned the right to play on [Fitzgerald’s] court. Prodigious research and fierce affection illumine every remarkable page.”


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I, like many others have given up with the game, dont get me wrong im still wining leagues and cups. but its just boring to watch
LOST INTEREST IN THE GAME.
The only way to get new players is if you buy them with actual money. If you're a new person to the game you cant even go to auction cause its to expensive and you barely even win a match cause you're players training takes to long i would be embarrassed if is was nitro for creating this i love rugby but this is a game where you buy to win.


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have many advantages...
With a comprehensive quality system we make sure that we meet food safety requirements from Danish and European authorities...read more
...go many years back in time, and throughout the years we have gone through many changes - but we still only produce chicken ...read more
When speaking of animal welfare we put focus on avoiding unnecessary suffering.
It is not acceptable when dogs, horses or other animals suffer, and Danish farming has a tradition of doing things properly.
Danpo A/S is part of the poultry group Scandi Standard, which is listed on the Stockholm Stock Exchange. ...About us
The reason for having our chickens approved as halal is decades of successful export to Muslim customers that value the high quality of Danish chicken....read more

domain: food


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Volume 2, Issue 2, Autumn 2016
Trajectories of Modernity
Elaborating the Philosophical Dimensions
The Development of Historical-Comparative Sociology in Johann Pall Arnason’s Civilizational Analysis
This article deals with the work of the Icelandic sociologist and philosopher Johann Pall Arnason (1940–) and with his concept of ‘civilizational analysis’. More precisely, I want to show that Arnason’s original interpretation of civilizational analysis goes beyond the mainstream understanding of historical sociology as a dialogue between history and sociology in favour of philosophical approaches. By outlining the three levels of Arnason’s civilizational analysis—(I.) the reconstruction of the concept of ‘civilization’, including its history, (II.) the link to philosophical sources and (III.) the theoretical development of Eisenstadt’s heritage—the article shows that, according to Arnason, the concept of ‘civilization’ is understood as amenable to the historical-sociological concepts of ‘culture’ (Max Weber, Shmuel N. Eisenstadt) on the one hand, and the philosophical concepts of the ‘world’ (Maurice Merleau-Ponty, Jan Patočka, Cornelius Castoriadis) on the other. Arnason thus, defining ‘culture’ as the so-called ‘interpretive articulation of the world’, emphasizes the motif of ‘cultural creativity’, which is present, yet theoretically underdeveloped, both in Weber’s and Eisenstadt’s work. In this light, the article finally focuses on Arnason’s most recent attempts to discuss Eisenstadt’s concept of the ‘civilizational dimension’ of modernity. It deals primarily with the terms ‘cultural ontology’ and ‘civilizational paradox’, in which the need to link historical sociology to philosophical perspectives is most evident. The civilizational approach is thus introduced as the crucial framework of Arnason’s elaboration of the philosophical dimensions in sociological analysis.


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What recipe is low in calories but good in tase?
Are you channeling your best self with this comment? (If you're not sure, check out our Code of Conduct.)
I made this Mark Bittman gazpacho last night and it was amazing!
Sam is a trusted home cook.
A Thai salad. With a cabbage slaw mix, Tomatoes, green onions, Hot Peppers, with chopped chicken breast marinaded in garlic and lime juice (grilled and chopped). Dressed with a dressing of Ginger, fish sauce (or soy), oil, a touch of brown sugar, lime juice, and a pinch of white pepper.
Modica, Sicily had something for both of us.
Where to Eat in Modica, Sicily
30-Minute Chickpea Curry
What's New in the Neighborhood
Cooking on a Food Stamp Budget
The Hits Keep Coming