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Alberta is home to Canada’s first national park and its largest mountainous park. The province is also home to North America’s largest shopping and entertainment complex, the world’s first international peace park, the largest ski area in North America, the first North American fringe theater festival and one of the world’s largest known deposits of dinosaur fossils. It is a year-round destination with a variety of recreational activities, sports events and cultural activities that visitors can enjoy at reasonable prices — it is the only province in Canada with no provincial sales tax.
The challenge of building a perfect Alberta itinerary comes in selecting the attractions and activities that are best suited to the season. Here are some suggestions — in each of the four seasons — to consider when creating an ideal itinerary for clients.
FallIn the fall, clients should start with a three-night stay in Kananaskis. After a flight to Calgary, it’s a 90-minute drive away. Wildlife viewing is a good way to start an itinerary — thousands of golden eagles fly over Kananaskis Country during the fall migration. Agents can arrange for a tour by a local guide or suggest that clients head to Hay Meadow where scientists conduct research on birds.
Next, clients can take an aerial tour of the region. Kananaskis Helicopter Tours offer a 60-minute alpine helicopter tour, or the more adventurous can choose a heli-hiking adventure.
Another activity to suggest during a stay at Kananaskis is day-hiking. Clients can head to the Grassi Lakes Trail near Canmore (a waterfall, two lakes and a petroglyph are highlights). Other possibilities include cycling on a paved or mountain trail, golf at one of the three area courses or a visit to the spa at the Delta Lodge at Kananaskis.
On day four, clients can drive from Kananaskis to Banff for a three-night stay. Start their Banff experience with more wildlife viewing and suggest that they take a day trip to Lake Louise and Moraine Lake, watching for bull elk along the way — fall is mating season. Another activity to recommend is a hike to Larch Valley from Moraine Lake. This is particularly stunning in fall when the larch trees begin to drop their needles.
Clients should also be sure to ride the Banff Gondola, which will provide them with some of the best views in the Canadian Rockies. Other suggestions include a mountaineering or climbing excursion, a soak in the Banff Upper Hot Springs, a trail ride, shopping on Banff Avenue, golf at the Banff Springs course or a spa treatment at one of several local spas.
For a longer stay, suggest a drive along Icefields Parkway to Jasper where clients can spend a few days exploring the glaciers before flying home from Edmonton.
WinterClients should begin a winter itinerary in Edmonton to check into the Fantasyland Hotel inside the West Edmonton Mall for a two-night stay. Suggest they visit the indoor water park, the indoor amusement park and the shops, dining and entertainment venues inside the mall.
On day three, clients can head to Jasper for three nights either by car or by train. An ideal way to get to Jasper is on Via Rail Canada (www.viarail.ca). One of the most stunning winter activities is the Maligne Canyon Ice Walk in the Canadian Rockies. Agents can book a walk with any of the following operators: Jasper Adventure Centre (www.jasperadventurecentre.com), SunDog Tours (www.sundogtours.com) or Walks and Talks Jasper (www.walksntalks.com).
After an ice walk, clients can continue their outdoor adventure at one of Jasper’s natural ice-skating rinks. Skate rentals can be found at the Fairmont Jasper Park Lodge or at Pyramid Lake. Another popular outdoor winter activity is cross-country skiing. With more than 185 miles of cross-country ski trails, Jasper is one of the largest cross-country ski destinations in Canada.
Other possibilities clients can consider are downhill skiing at Marmot Basin, snowshoe tours, dogsled adventures, guided winter wildlife walks, ice climbing or guided cross-country ski trips. Recommend they visit the Jasper Adventure Centre to book an activity.
For a longer stay, suggest that they drive to Banff and spend a few days exploring before flying home from Calgary.
SpringStart a spring Alberta adventure in Banff, which is within driving distance of Calgary. Agents should suggest that clients stay for approximately four days to get the full feel of the destination. Visitors can start their journey with spring skiing. Clients can purchase a tri-area ski pass and enjoy three area resorts — Sunshine Village, The Lake Louise Ski Area and Mt. Norquay (www.skibig3.com).
Other possibilities include caving in the Rat’s Nest Cave with Banff Adventures (www.banffadventures.com); riding the Banff Gondola, a soak in the Banff Upper Hot Springs, shopping on Banff Avenue or a visit to one of several area spas.
On day five, clients can head to Drumheller for a two-night stay and to explore the Alberta Badlands. Suggest that they start with a stop at the Royal Tyrell Museum to see the 110,000 fossil specimens and 40 dinosaur skeletons. The next stop should be a hike in the Hoodoos, which are found in the Alberta Badlands and form a landscape that seems otherworldly. Next, suggest they check out some of the trails in Dinosaur Provincial Park.
On day seven, visitors should make the 90-minute drive back to Calgary. For a longer stay, suggest they drive south to visit Writing on Stone Provincial Park where they can see an amazing collection of First Nations petroglyphs and pictographs before returning to Calgary.
SummerIn the summer, suggest clients head to Calgary during the Calgary Stampede (www.calgarystampede.com). On day one, clients arrive in Calgary and stay at an area hotel for three nights to experience the event. They can enjoy free pancake breakfasts, parades, midways, the Indian Village, agricultural displays, western hospitality and top rodeo action at the “Greatest Outdoor Show on Earth.” On day four, suggest that visitors travel by car from Calgary to Kananaskis for their own western adventure with a multi-day horseback pack trip with Brewster Adventures (www.brewsteradventures.com). They can also enjoy a three-night stay at the Outpost at Warden Rock, which is accessible on horseback or via a genuine stagecoach (www.outpostatwardenrock.com). Another option is a stay at an area guest ranch, such as Rafter Six Ranch or the Brewster Kananaskis Guest Ranch (www.raftersix.com or www.kananaskisguestranch.com).
Keep clients busy by suggesting that they go whitewater rafting, canoeing or kayaking on the Kananaskis River. Clients can also go hiking or golf on one of three area courses. On day seven, they can return to Calgary or, for a longer stay, clients can spend a few days exploring Banff and Lake Louise.


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Allow me to direct you to a new election website:
The message is this: Don't vote to make future generations poorer, as the Greens' billboards exhort you to: vote instead for freedom, vote for prosperity, vote (in short) for yourself.
Which means Don't Vote Greens.
And whatever you do, Don't Vote National.
National are NOT the answer.

domain: sociology


You can apply for a licence if you need to control wild birds to prevent disease or serious damage to livestock, foodstuffs for livestock, crops, vegetables, fruit, growing timber, fisheries or inland waters.
Criteria you must meet to get a licence
Natural England will only issue you with a licence if you can show that:
- actual damage or a problem is occurring, or likely to occur – and the target species is causing it
- you have tried non-lethal solutions or you can show that they would not be effective or practical
- there are no suitable alternative methods of control
- action you take under the licence will contribute to preventing damage or resolving the problem
- action you take under the licence will not negatively affect the conservation status of the wild bird
If your application does not meet all these criteria, Natural England will reject your application.
Using the licence
You can use this licence to control wild birds, their nests and eggs by:
- disturbing them
- killing them
- taking them
- using a prohibited method of control on them
If you use a trap or decoy bird, you must follow the standard licence conditions in WML-GL33.
Before you apply
Before you apply for a licence, check if your circumstances are covered by one of Defra’s general licences for birds.
You may be able to control wild birds using alternative non-lethal measures. Read about the legal methods you can use to deter certain wild birds without a licence (GU01).
Control birds at an airfield
If you need to control birds at an airfield, check if the air safety class licence CL12 covers your circumstances.
If the air safety class licence CL12 does not cover your circumstances, submit an A08 application form to control wild birds for air safety purposes.
Control wild birds for conservation or health and safety
To apply for a licence to control wild birds for conservation or health and safety purposes that are no longer covered by a general licence, you need to submit an A09 screening form.
Apply for an A08 licence
To apply for a licence to control wild birds to prevent disease or serious damage, fill in:
- form A08 – available on this page
- section 8 extension form – if you have to apply for additional licensable actions
Send your forms to Natural England using the details on the application forms.
This licence is free.
Natural England aims to respond within 30 working days of accepting your application, but the decision can take longer at peak times. Natural England will contact you if it expects to take longer.
You must not start lethal control, or hire a contractor for lethal control, before Natural England has granted your licence.
Report your actions
You must report any actions you take using this licence. Use the report form template LR08 on this page.
You must report within 2 weeks of your licence expiring, even if you’ve taken no action.
Send your completed form to Natural England using the details on the form.
Renew your licence
You can use the report form to renew your licence.


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Belgian superstar DJ/Producers Dimitri Vegas & Like Mike have teamed up with trailblazer R3HAB and Italian pioneering electronic artist Prezioso to release their new single ‘Shooting Darts.
An original release lined with the production virtues Dimitri Vegas & Like Mike’s music has become renowned for, tied in with the inimitable style R3HAB has elevated in recent years and Prezioso’s unique flair with his music. A power chord record with a profound expressive energy, ‘Shooting Darts’ blends pop music with contemporary electronic music. The track glides across an emotional musical spectrum thanks to an elastic bassline and arresting female topline vocal.
The monolithic brotherly duo Dimitri Vegas & Like Mike and ambassadors of the worldwide phenomenon Tomorrowland have taken the world by storm over the past decade. From becoming the first-ever duo to win the World #1 DJ’s crown at the prestigious Top 100 DJs Poll in 2015, the twosome continued to stand firm and managed to stay in the top two for seven years, even claiming the World No.1 crown for a second time (in 2019). Clocking up more than 30 million fans on social media, combined with billions of music streams and video views of their live performances and music. The duo continues to climb global charts and work with everyone from Diplo and David Guetta to Paris Hilton and Natti Natasha to Hans Zimmer and Wiz Khalifa.
Dutch/Moroccan Multi-Platinum DJ and producer Fadil El Ghoul, better known as R3HAB, has been trailblazing his way through dance music for the past decade. From his breakthrough remixes for Rihanna, Drake, Taylor Swift, Calvin Harris, to anthems such as Platinum-certified ‘All Around The World (La La La)’ and ‘Lullaby,’ to his collaborations with the likes of Ava Max, Luis Fonsi, Sean Paul, and more, R3HAB has proven to be one of the most forward-thinking artists in the game. His work has collected over 5 billion streams to date, including 1.1B in 2021 alone. R3HAB founded his label CYB3RPVNK in 2016, which has passed 4B streams across platforms since its inception. He is Spotify’s Most Discovered Artist, one of Spotify’s top 200 most streamed artists, ranked #12 on the prestigious Top 100 DJs Poll, and has earned 55 Gold and Platinum certificates. In addition, R3HAB has embarked on multiple sold-out worldwide tours, performing at the world’s top festivals, such as EDC, Ultra Music Festival, Balaton Sound, Coachella, Summer, Sonic Festival and Tomorrowland.
Italian DJ/producer Prezioso, real name Giorgio Prezioso, is a talented turntablist and winner of numerous DJ competitions. Since the 90s, Prezioso has been making waves in the industry both home and abroad at the forefront of dance music. Most known for his multi-awarded single ‘Thunder’, which has more than 250 million streams combined, Prezioso has also released influential singles such as ‘Tell Me Why’, ‘Let Me Stay’ and ‘The Riddle’.

domain: news


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“My Freshman Year: What a Professor Learned by Becoming a Student” (2005, nonfiction) was written by Rebekah Nathan. Actually, this is the author’s pseudonym. A professor of anthropology at a large state university, the author was struggling to understand why some students didn’t participate in class discussions, didn’t complete the assigned reading, and didn’t seek out assistance during her office hours. Drawing on her fieldwork experience, she decided to go “underground.” She took a sabbatical from her job and applied to her own university as a freshman, on the merits of her high school transcript. After being accepted, she moved into a residence hall, took classes, and got involved in student life. Her new and very different interactions with students enabled her to better understand the lives of college freshmen as well as the challenges they face.
The staff of my office, the Office of Academic Support at a private four-year university, read this book and discussed it over a series of four weeks. This item contains the discussion questions we used for each of the four weeks, as well as a timeline indicating the chapters and number of pages. I have also included an alternative timeline that spans just three weeks. The questions are intended to promote reflective discussion, encouraging higher education professionals to consider how the experiences of students at the author’s university compare to their institution and their students.
Although this item is intended for educators, the questions could be adapted for use by students who read the book.

domain: library


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I'm at work today at the Martin Luther King, Jr. Memorial Library at 901 G Street, NW in Washington, DC. I just went outside to mail some letters and the temperature is approaching 100* F. Out on the library's plaza I came upon this site.
I felt so sorry for these dogs. The black puppy looked absolutely lethargic. There was a bowl of water.
The owner was certainly inside the library. Gotta wonder where he is peddling from and where he is going.


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A new study, led by researchers at Intermountain Medical Center in Salt Lake City, found that using advanced clinical decision support (CDS) tools can reduce mortality for pneumonia patients.
The researchers studied the impact of an advanced computer program designed at Intermountain, which combs through a patient's medical information and risk factors in real time to alert an emergency department physician if the patient likely has pneumonia. Once the diagnosis is confirmed, it calculates severity assessment and provides management recommendations based on current North American guidelines. They found that use of the tool saved up to 12 lives over the course of a year, compared to routine care standards.
The tool can also process the radiologist's chest imaging interpretation to identify patients with radiographic evidence of pneumonia, presence of multi-lobar disease, and pleural effusion. It also extracts prior microbiology results for bacteria that are resistant to antibiotics.
"This tool doesn't take over for doctors, but it does assemble the needed information, calculates the patients' severity of illness and likelihood of infection with resistant bacteria, and presents recommendations to help doctors make better decisions. It's all about giving local doctors tools to be more consistent, objective, and focused on best practices," states lead author Nathan Dean, M.D., a pulmonologist and chief of critical care medicine at Intermountain Medical Center.


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With the help of their high school’s newest teacher, Fredi Cameron (George Lopez), four Hispanic students form a robotics club. Although they have no experience, the youths set their sights on a national robotics contest. With $800 and parts scavenged from old cars, they build a robot and go up against the Massachusetts Institute of Technology (MIT), the reigning champions. Along the way, the students not only learn how to build a robot, they learn something far more important: how to forge bonds that last a lifetime.


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SBB Cargo and Hupac found SBB Cargo International.
Press release, 05.07.2010
SBB Cargo and the intermodal operator Hupac are jointly founding an independent, neutral company to handle transalpine transit freight between Germany and Italy. It is hoped that a clockface production system and lean structures will make the new company the cost leader on the north-south corridor.
As of the beginning of 2011, SBB Cargo and Hupac will be founding a new train operating company that will focus on block and intermodal trains on the European north-south corridor between Germany and Italy. In taking this step, the Board of Directors of SBB has affirmed the decision it took in February on the strategic realignment of SBB Cargo.
The shareholders in the new company will be SBB Cargo (75%) and Hupac (25%), the leading intermodal freight operator on the north-south corridor. It is possible that further partners will also join. The new company will intensify the existing decades-long partnership between the two companies. The aim is to jointly establish and develop a strong, neutral traction company that is close to its market. This move also seeks too achieve cost leadership in the intermodal segment by optimising resources on selected routes and halving structural costs. Since the company will be Swiss, quality will be a major priority.
The new company’s customers will primarily comprise intermodal operators, who will account for about 80% of the planned total sales of CHF 300 million. Although Hupac will itself be the company’s largest single customer, the range of services is clearly geared to other large customers. Apart from operators, these include railfreight and other railway companies and individual large-volume shippers. “The crucial thing is to be able to offer the market traction services between Germany and Italy as an independent, neutral train operating company. This is in the interest of both shareholders", explains Nicolas Perrin, Head of SBB Cargo.
Several railfreight operators dominate the north-south rail corridor, and a number of other railway companies now operate their own freight trains on the corridor in addition to SBB Cargo. The intense competition is creating heavy price pressure and slender margins for traction operators. “The step forward that has now been adopted will make it possible for us to continue to operate on the central European freight axis as a Swiss company. Looking ahead to the opening of the Gotthard base tunnel, Switzerland’s two strongest players in the sector need to pool their strengths. Only by doing so will we have a chance of actively counteracting the pressure being exerted by foreign operators with a solution of our own”, says Hans-Jörg Bertschi, Chairman of the Board of Directors of Hupac.
Neutral traction services on the same terms for all customers
Base on the north-south corridor
The spin-off of international operations into the new company will result in 157 redundancies at SBB Cargo over the next two years. These will be implemented in a socially responsible manner. SBB will offer alternatives for retraining within the rest of the company and can provide new employment opportunities thanks to growth in other areas. The best possible use will be made of all available solutions throughout the Group employment market, including offers to work in other SBB divisions, exploiting staff turnover, early retirement opportunities and, where necessary, outplacement. No staff who are employed under the collective employment contract will be made redundant.
The new company will probably be based in the Basel-Olten-Lucerne area. A search for suitable office premises is in progress. The existing depots on the north-south corridor in Switzerland will be retained, but – in a departure from current practice – drivers will be deployed to a greater extent within the same country. Basel will become the central hub of the Gotthard and Lötschberg corridor production concept. The number of drivers at SBB Cargo’s depots in Ticino and central Switzerland will remain at around its present level. In Germany, the staff currently based in Offenburg will be transferred to Mannheim in the medium term. The other sites in Germany and Italy will be retained for the time being. The subsidiaries currently responsible for production in Germany and Italy will be integrated into the new company. Chemoil, the subsidiary specialising in the transport of petroleum and chemical products, will remain an affiliate of SBB Cargo AG.
Sustainable business success with new production system
Full range of services from SBB Cargo in Switzerland
SBB Cargo intends to use its new “Rail & Transship” product to expand its position in intermodal transport in its domestic market. Looking to the long term, SBB Cargo is examining innovative solutions for comparatively short distances within Switzerland. Traffic from the North Sea ports will play a major role in import and export activities, and the construction of the planned new Limmattal Gateway terminal in Dietikon will be essential in securing this traffic.
Standardisation and alignment with customer requirements
Standardisation will enable the company to introduce uniform software, optimise processes and improve capacity management. SBB Cargo will only be able to cover the costs of operating its domestic network if it succeeds in scaling all its resources correctly and using their capacity more efficiently. Simultaneously, structural costs for the Swiss part of SBB Cargo's activities will have to be reduced to normal industry levels in the long term. SBB Cargo in Switzerland will make the transition to a process-driven organisation at the same time as its international business is spun off into a separate company. The necessary organisational changes will take place in the second half of 2010.
- Presentation from the press conference (PDF, german)


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The time is always right to do what is right.
"But I'm afraid." Working in mental health, I hear that regularly. But over the last year, it's something I hear much more frequently, sometimes even daily. Fear is a basic human survival tool. A perceived threat triggers the emotion, signaling our bodies to respond to the danger with a fight or flight response. It is an essential reaction to keep us safe.
Levantar, meaning to stand or to rise up in Spanish, brings culturally and linguistically tailored mental health care to Portland’s Latinx community.
Addiction to anger is being fed by some of our most widely used technologies.
"I am amazed at who I have been able to become."


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Harry Kane will make his eagerly awaited Tottenham return against Arsenal in the north London derby, but Dele Alli misses out at the Emirates Stadium.
England international Kane has been sidelined since September 18 with an ankle injury and his return to the starting XI is a timely boost for a Spurs side that are without a win in their last six matches in all competitions.
However, midfielder Alli is unavailable against Spurs’ nearest rivals after sustaining a knee injury in training on Friday with Kevin Wimmer coming in to take his place.
Arsene Wenger has made three changes to his line-up, with Nacho Monreal, Granit Xhaka and Theo Walcott all returning for Arsenal.
— Premier League (@premierleague) November 6, 2016
- Soccer News Like
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Dane and Scotty
Dane came to me with the idea to create an engagement ring for his partner Scotty, but, before we could finish the ring - Scotty went and surprised Dane by proposing to him instead!
They were obviously both on the same page, and so we ended up making a ring for each of them.
We designed the rings to be matching but different, Dane’s ring reflecting his Māori heritage, while Scotty’s incorporates the Scottish thistle to honour his.
We used 3D technology to create the designs on the rings and this allowed us to achieve very the fine and precise lines for the details.
And we then finished the edges by hand with a hammering process to give both rings a rustic and masculine feel.
Finally we cast them in solid 18 carat Yellow Gold for timeless quality.
The final result is two rings that are unique and individual, and also represent the coming together of two people and two cultures.
It’s my passion to create rings like this that intertwine into peoples lives and stories, and will be worn with pleasure for a lifetime.

domain: artistic


AT&T has introduced an upgraded version of DirecTV for business customers, including hotels, that features a large lineup of HD channels, 4K capabilities and multiscreen access.
The third-gen platform, called COM3000, supports up to 138 HD channels (along with select programming in 4K and HDR formats), and lets business owners tack on their own in-house and OTT channels.
In addition to TVs, COM3000 is also capable of supporting smartphones and tablets, select smart TVs and third-party set-top boxes. The service can also be delivered on a range of access infrastructures, including coax, Ethernet and WiFi, AT&T said.
AT&T launched COM3000 at this week’s Hospitality Industry Technology Exposition & Conference, happening this week in Houston. Here’s a sampling of other announcements made HITEC:
The smarter way to stay on top of the multichannel video marketplace. Sign up below.


UN Secretary General Antonio Guterres yesterday raised concerns that a concrete dome built last century to contain waste from atomic bomb tests is leaking radioactive material into the Pacific.
Speaking to students in Fiji, Guterres described the structure on Enewetak atoll in the Marshall Islands as “a kind of coffin” and said it was a legacy of Cold War-era nuclear tests in the Pacific
“The Pacific was victimised in the past as we all know,” he said, referring to nuclear explosions carried out by the United States and France in the region.
In the Marshalls, numerous islanders were forcibly evacuated from ancestral lands and resettled, while thousands more were exposed to radioactive fallout.
The island nation was ground zero for 67 American nuclear weapons tests from 1946-58 at Bikini and Enewetak atolls, when it was under US administration.
The tests included the 1954 “Bravo” hydrogen bomb, the most powerful ever detonated by the United States, about 1,000 times bigger than the atomic bomb dropped on Hiroshima.
Guterres, who is touring the South Pacific to raise awareness of climate change issues, said Pacific islanders still needed help to deal with the fallout of the nuclear testing.
The “coffin” is a concrete dome, built in the late 1970s on Runit island, part of Enewetak atoll, as a dumping ground for waste from the nuclear tests. Radioactive soil and ash from the explosions was tipped into a crater and capped with a concrete dome 45 centimetres (18 inches) thick.
However, it was only envisaged as a temporary fix and the bottom of the crater was never lined leading to fears the waste is leaching into the Pacific.
Cracks have also developed in the concrete after decades of exposure and there are concerns it could break apart if hit by a tropical cyclone.


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Talks to stem retrenchments in South Africa's mining sector and improve bureaucratic efficiencies were welcomed by the Chamber of Mines.
The National Council of Provinces is to vote on the controversial changes to the MPRDA on March 27.
The humanitarian crisis at west Johannesburg mine, Blyvooruitzicht, would be the focus of an urgent meeting led by Ngoako Ramatlhodi, SA's mines minister.
A strike over pay at South African gold mines could lead to renewed violence in the country's mining sector.
Chilean President Sebastian Pinera is jubilant about the current rescue operation being conducted at the San Jose mine, but cautiously warned that the rescue the saga wouldn't be over until all 33 men are out.
The Chamber of Mines welcomed comments by finance minister Nhlanhla Nene that the mining sector must get "on the front foot" nationally and internationally.
Lynne Brown, public enterprises minister, would seek a chartered accountant for Eskom's board as she sought to unravel the mystery of its finances.
The price of gold advanced to three-week highs as Asian investors sought to buy physical stores of the metal.
London Mining is hoping to raise $400m to expand its Marampa iron ore mine in Guinea to 8 million tonnes a year.
Royal Bafokeng Platinum has cut its contract with Shaft Sinkers on the Styldrift 1 project after the services firm's CEO and CFO left the company.


Centuries-old Indian medical system Ayurva requires to be spread all over the world to get integrated into the public health systems to save healthcare expenditure in many countries, international health experts said in New Delhi on Saturday.
Natalia Marzoa Silva of the Cuban health ministry said her government is eager to include Ayurveda in primary and higher medical education in Cuba.
“Ayurveda can have a place in the healthcare system of Cuba. Ayurveda can be utilised to treat cancer in our country as the disease has become the third biggest cause of deaths,” she said.
Silva was speaking at a plenary session, Global perspective on Ayurveda, at the ongoing World Ayurveda Congress in New Delhi.
“A large number of people come to India to study Ayurveda, but they return armed with diplomas after studying for only three months,” said another speaker Gregor Kos from the health ministry of Slovenia.
“We will work with the Department of Ayush under the Union Ministry of Health to put quality control in place,” added Kos.
Health officials also called for strict quality control in traditional medicines in the backdrop of an increasing number of people abroad willing to be tested by Ayurveda specialists.
Maldives health minister Mohammed Habeeb said: “I am eagerly waiting for the day when Ayurveda will be a prominent part of the healthcare system of my country.”
Indian Health Minister Harsh Vardhan, who chaired the session, said: “We are determined to increase international cooperation in traditional medicine by signing MoUs with other countries to render cooperation in the field of research and for standardisation of the medicine system and practice.”
He said that a MoU with Malaysia in the field of traditional medicine has been signed under which the health ministries of the two countries have already held two bilateral technical meetings to enhance cooperation.
“India has also instituted 20 seats each for students from South East Asian countries to study Ayurveda in Indian educational institutions,” he said.


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Finn Funko Pop Dolls are finally here!
|Britnee from @libraryofthenight has done it again! She created this gorgeous Finn Funko, the dreamy golden haired, green eyed Scottish druid and main heartthrob of my Her Dark Destiny Series! Don’t miss your chance to add Finn to your pop doll collection, especially if you were lucky enough to grab the Fate Funko back in April. |
PLUS, I’m gifting a Gabriella Budjoso art card, signed by me on the back with every purchase!
CLICK HERE to get your Finn Funko collectible with signed Gabriella Bujdoso art card gift while quantities last.
Remember to use code TRAE10 for a 10% discount at checkout!


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The best Side of limo gini
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domain: medical


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These are the new movies being released on DVD this Tuesday.
Quantum of Solace (Action/Thriller)
On a mission that leads him to Austria, Italy and South America, Bond discovers that Greene, conspiring to take total control of one of the world’s most important natural resources, is forging a deal with the exiled General Medrano. Using his associates in the organization, and manipulating his powerful contacts within the CIA and the British government, Greene promises to overthrow the existing regime in Bolivia, giving General Medrano control of the country in exchange for a seemingly barren piece of land which is, however, a main source of the South American water supply. In a minefield of treachery, murder and deceit, Bond allies with old friends in a battle to uncover the truth. As he gets closer to finding the man responsible for the betrayal of Vesper, 007 must keep one step ahead of the CIA, the terrorists, and even ‘M’ herself, to unravel Greene’s sinister plan and stop Quantum from getting its way.
Cast: Daniel Craig, Olga Kurylenko, Gemma Arterton, Judi Dench, Jeffrey Wright, Giancarlo Giannini; Directed by: Marc Forster
For super-dog Bolt (voice of John Travolta), every day is filled with adventure, danger and intrigue-at least until the cameras stop rolling. When the star of a hit TV show is accidentally shipped from his Hollywood soundstage to New York City, he begins his biggest adventure yet-a cross-country journey through the real world. Armed only with the delusions that all his amazing feats and powers are real, and with the help of two unlikely traveling companions: a jaded, abandoned housecat named Mittens (voice of Susie Essman) and a TV-obsessed hamster in a plastic ball named Rhino, Bolt discovers he doesn’t need superpowers to be a hero.
Cast: Featuring the Vocal Talents of: Miley Cyrus, John Travolta, Malcolm McDowell, Diedrich Bader, Susie Essman, James Lipton, Randy Savage; Directed by: Byron Howard, Chris Williams
View the Bolt movie trailer

domain: entertainment


text:
(ConservativeSense.com) – The National Rifle Association (NRA) is accusing leading Democrat Chuck Schumer of flouting the Constitution with his proposals to ban assault weapons. The leader of the Democratic Party in the Senate wrote on social media that he plans to reintroduce a bill to prohibit certain firearms in a revival of an assault weapons ban that expired 20 years ago. “After I led the passage of the Brady Bill and the Assault Weapons Ban 30 years ago, America saw a decrease in mass shootings and gun deaths,” he said.
Congress enacted the Brady Handgun Violence Prevention Act in 1993, which imposed a nationwide ten-year ban on semi-automatic firearms and large-capacity ammunition magazines. The legislation expired in 2003, but Democrats, including President Biden, have made clear their intent to reinstate it. While serving as a Delaware Senator, the President voted in favor of the Brady legislation, and in September, he told reporters he would not “stay quiet” until similar legislation was in effect.
“The American people are sick and tired of enduring one mass shooting after another,” Schumer said before calling on his Congressional colleagues to “end the scourge of gun violence in America.”
The NRA is backed by Republican Wyoming Senator John Barrasso, who said the Democrats’ “bumper sticker solution to ban guns” is not a matter of public safety but infringing freedoms. Mr. Barrasso tweeted that he would block attempts to interfere with constitutional rights by a party that made America less safe with its attacks on law enforcement through police defunding.
NRA Executive Director Randy Kozuch thanked Senator Barrasso for his stance and issued a statement praising him for standing up for the rights of law-abiding gun owners, who would bear the brunt of firearms-limiting legislation.
Despite claims from both Biden and Schumer that assault weapons bans reduce gun violence, studies in 1999 and 2004 found they had little impact. The Department of Justice said in 2004 that the “effects on gun violence are likely to be small at best.”
Copyright 2023, ConservativeSense.com


text:
Les Gîtes Makadan1728 Rue Saint-Cyrille, Normandin, QC G8M 4K5, Canada
Solo, as a family, couple or group, you will be charmed.
Glass pane lounge, exterior swimming pool. Close to the Véloroute des Bleuets (shuttle restaurant 3km.) Garage for bicycles and motorcycles. Located four kilometers from the Grands Jardins de Normandin.
2001 National GOLD Tourism Awards winner
- Bedrooms: 5
- Private bathrooms: 4
- Shared restrooms: 2
- Check-in After: 16h00
- Opening schedule: Open year-round
- Classification: ☀☀☀☀
- CITQ #: 120503
- Price for 2 persons: 90$ Daily
- Additional guests: 20,00$
- Air conditionning
- Credit Card

domain: travel


text:
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Throughout alcoholic fermentation, nitrogen depletion is one of the most important environmental stresses that can negatively affect the yeast metabolic activity and ultimately leads to fermentation arrest. Thus, the identification of the underlying effects and biomarkers of nitrogen limitation is valuable for controlling, and therefore optimizing, alcoholic fermentation. In this study, reactive oxygen species (ROS), plasma membrane integrity, and cell cycle were evaluated in a wine strain of Saccharomyces cerevisiae during alcoholic fermentation in nitrogen-limiting medium under anaerobic conditions. The results indicated that nitrogen limitation leads to an increase in ROS and that the superoxide anion is a minor component of the ROS, but there is increased activity of both Sod2p and Cta1p. Associated with these effects was a decrease in plasma membrane integrity and a persistent cell cycle arrest at G0/G1 phases. Moreover, under these conditions it appears that autophagy, evaluated by ATG8 expression, is induced, suggesting that this mechanism is essential for cell survival but does not prevent the cell cycle arrest observed in slow fermentation. Conversely, nitrogen refeeding allowed cells to reenter cell cycle by decreasing ROS generation and autophagy. Altogether, the results provide new insights on the understanding of wine fermentations under nitrogen-limiting conditions and further indicate that ROS accumulation, evaluated by the MitoTracker Red dye CM-H2XRos, and plasma membrane integrity could be useful as predictive markers of fermentation problems.
During wine fermentation, yeast cells are exposed to numerous environmental stresses, which could have adverse effects on cell growth and ultimately may lead to fermentation arrest. In recent years, studies of the yeast transcriptome (5, 20, 22, 30, 36) and/or proteome (34, 38) contributed to a greater understanding of how wine yeasts cope with the various stressful conditions that arise during winemaking. In general, these studies have shown that entrance into stationary phase is the landmark event during wine fermentation, resulting in major transcriptional changes characterized by a general stress response (22). Reactive oxygen species (ROS), including the superoxide anion (O2−), hydrogen peroxide (H2O2), and the hydroxyl radical (OH•), are highly toxic oxidants frequently generated by cells in a wide variety of metabolic pathways during normal metabolism. Although ROS have not been considered key determinants of alcoholic fermentations, there is evidence that ROS production is associated with fermentation (12, 17, 31) and that the production of ROS may also be part of the yeast cell response to a variety of stressors that occurs during must fermentations (26). In this context, ROS accumulation may cause oxidative damage to proteins, nucleic acids and lipids, and other cellular components (35), eventually leading to yeast cell death.
Recently, a genome-wide expression study showed that yeast cells respond to the challenge of nitrogen limitation by inducing a great number of genes of respiratory metabolism, for example, those associated with the tricarboxylic acid cycle, oxidative phosphorylation, and oxidative stress response, despite the large amounts of glucose present in the medium (22). In addition, the upregulation of genes involved in cell budding and DNA synthesis is indicative of yeast cell proliferation and suggests that the yeast population is in an unsynchronized state. As found for the majority of eukaryotes, in budding yeast cells the decision to commit to another division cycle, enter a quiescent state, or enter into meiosis takes place during the G1 phase of the cell cycle (16). Yeast cells resume proliferation when nutritional conditions are favorable but prepare themselves to enter into a resting phase (G0) under conditions where essential nutrients start to become limiting (37). On the other hand, oxygen availability is known to affect cellular and fermentation processes (28, 29).
The aim of the current study was to obtain new insights on the responsiveness of yeast cells under nitrogen-limiting conditions and specifically to evaluate potential biomarkers that could be used to predict fermentation problems and to optimize fermentation during alcoholic beverage production. To achieve these aims, the following parameters were evaluated under anaerobic conditions in a wine strain of Saccharomyces cerevisiae growing in a nitrogen-limited medium mimicking enological conditions: ROS production, plasma membrane integrity, cell cycle profile, and autophagy induction. The effects of nitrogen addition to a nitrogen-limiting fermentation were also evaluated.
The wine yeast strain S. cerevisiae PYCC4072 was supplied by the Portuguese Yeast Culture Collection (PYCC) and maintained at 4°C on slants of yeast peptone dextrose agar (YPD) medium containing glucose (20 g liter−1), peptone (10 g liter−1), yeast extract (5 g liter−1), and agar (20 g liter−1). Fresh cells grown on YPD slants for 24 h were used in all experiments.
The experimental design was based upon the conditions previously described to ensure continuity in the studies (22) with some modifications. Yeast strain S. cerevisiae PYCC4072 was grown in chemically defined grape juice medium (GJM) formulated by Henschke and Jiranek (14). Glucose (200 g liter−1) was used as the sole carbon and energy source, and nitrogen was supplied as diammonium phosphate. Briefly, three fermentation conditions with 200 g liter−1 of glucose and different initial nitrogen concentrations were carried out: (i) with 267 mg of N liter−1 (GJM267), the nitrogen concentration required by the yeast strain to complete fermentation (control fermentation); (ii) with 67 mg N liter−1 (GJM67), the nitrogen concentration that leads to slow fermentation (nitrogen-limiting fermentation); and (iii) with the addition of 200 mg of N liter−1 onto the nitrogen-limiting fermentation 72 h after inoculation (GJM67+200), coinciding with 48 h of nitrogen depletion (refed fermentation).
For all experiments starter cultures were prepared by growing the yeast cells overnight in 250-ml flasks containing 100 ml of GJM267. The flasks were incubated at 25°C in an orbital shaker set at 150 rpm. The experimental cultures were inoculated with 5 × 105 CFU ml−1 of starter culture. Fermentation was carried out in 500-ml flasks filled to two-thirds of their volume, fitted with a side-arm port sealed with a rubber septum for anaerobic sampling, and maintained at 20°C in an orbital shaker at 120 rpm, according to the methodology in use in our laboratory and described elsewhere (21). Aseptic sampling was accomplished using a syringe-type system. To avoid medium accumulation in the system, a stylet was inserted in the needle holder. Each flask was closed with a rubber stopper, allowing fermentation gasses to escape through a glass tube connected to a two-way valve by Teflon tubing; the other end of the tubing was connected to a tube with 10 ml of distilled water. Every 24 h, each flask was removed from the shaker and weighed. Samples were collected daily for assessing fermentation and growth parameters. Prior to sampling, the flasks were stirred to ensure homogeneity. The end of alcoholic fermentation was confirmed using Clinitest tablets (Roche).
Optical density at 640 nm (OD640) of appropriately diluted culture samples was used as an estimate of yeast cell growth. The number of viable cells was periodically determined using serial dilutions of the samples which were pour-plated in YPD medium. The number of CFU was counted after 48 h at 30°C. Loss of carbon dioxide was measured by weight loss. Glucose was quantified by the 2,4-dinitrosalicylic acid (DNS) method (25).
Plasma membrane integrity was assessed by flow cytometry using propidium iodide (PI) (Molecular Probes, Eugene, OR) vital staining as described elsewhere (8) with minor adaptations (3). Briefly, PI was added to yeast cell suspensions (106 cells ml−1) from a working solution (0.5 mg of PI in 10 ml of Tris-MgCl2 buffer) to a final concentration of 6.7 mg ml−1 and incubated at 37°C for 15 min. Cells with high red fluorescence were considered to have plasma membrane disruption.
Intracellular reactive oxygen species accumulation was monitored with the MitoTracker Red dye CM-H2XRos (Molecular Probes, Eugene, OR) essentially as described elsewhere (18) and with dihydroethidium (DHE) as a probe to detect superoxide anion. The reduced form of this dye does not fluoresce until it enters an actively respiring cell, where it is oxidized by ROS to a red fluorescent compound, which is sequestered in the mitochondria. Briefly, DHE was added to yeast cell suspensions (106 cells ml−1) from a working solution (2.5 mg of DHE in 1 ml of absolute ethanol) to a final concentration of 10 μg ml−1 and incubated at 30°C for 10 min in dark. The fluorescence was detected by flow cytometry.
Mitochondrial membrane potential was monitored by staining cells with the fluorescent dye rhodamine 123 (Rh123) essentially as described elsewhere (19). Rh123 is a cationic dye that distributes electrophoretically into the mitochondrial matrix in response to mitochondrial membrane potential.
Cell cycle analysis was performed in nitrogen-starved and nonstarved cells according to a previously described procedure (2). Briefly, at the desired time points, cells were harvested, washed, and fixed with ethanol (70%, vol/vol) for 30 min at 4°C, and this was followed by sonication, treatment with RNase for 1 h at 50°C in sodium citrate buffer (50 mM sodium citrate, pH 7.5), and subsequent incubation with proteinase K (1 mg per 106 cells). Cell DNA was then stained overnight with SYBR green (10,000×; Molecular Probes), diluted 10-fold in Tris-EDTA (pH 8.0), and incubated overnight at 4°C. Before cytometric analysis, samples were diluted 1:4 in sodium citrate buffer. Determination of cells in each phase of the cell cycle was performed offline with Cylchred and WINMDI software.
Fluorescence-activated cell sorter (FACS) analysis was carried out with an Epics XL-MCL flow cytometer (Beckman Coulter, Fullerton, CA) equipped with an argon ion laser emitting a 488-nm beam at 15 mW. The green and red fluorescence were collected through a 488-nm blocking filter. A 550-nm/long-pass dichroic mirror with a 525-nm/band-pass filter and a 590-nm/long-pass with a 620-nm/band-pass filter were used to detect green fluorescence and red fluorescence, respectively. An acquisition protocol was defined to measure forward scatter (FS log), side scatter (SS log), green fluorescence (FL1 log), and red fluorescence (FL3 log) on a 4-decade logarithmic scale. Data (20,000 cells per sample) were analyzed with the Multigraph software included in the System II acquisition software for the Epics XL/XL-MCL, version 1.0.
For determination of catalase and superoxide dismutase activities, yeast extracts were prepared in 25 mM Tris buffer (pH 7.4) containing a cocktail of protease inhibitors. Protein content of cellular extracts was estimated by the method of Lowry using bovine serum albumin as a standard.
Catalase activity was determined as previously described (1). Briefly, 30 μg of protein was separated by native PAGE, and catalase activity was analyzed in situ in the presence of 3,3′-diaminobenzidine tetrahydrochloride (Sigma), using the H2O2-peroxidase system. The gel was incubated in horseradish peroxidase (50 μg ml−1; Sigma) in 50 mM potassium phosphate buffer (pH 6.7) for 45 min. H2O2 was then added to a final concentration of 5 mM, and incubation was continued for 10 min. The gel was then rapidly rinsed twice with distilled water and incubated in 0.5 mg ml−1 diaminobenzidine in 50 mM potassium phosphate buffer until staining was complete.
Superoxide dismutase activities were measured based on their ability to inhibit reduction of nitroblue tetrazolium to formazan in nondenaturing polyacrylamide gels (9). Sod2p activity was distinguished from Sod1p activity based on the ability of 2 mM cyanide to inhibit Sod1p but not Sod2p. Quantification of band intensities was performed by densitometry using Quantity One Basic Software from Bio-Rad.
RNA was obtained from samples periodically collected in the slow (GJM67) and control (GJM267) fermentations as well as in refed fermentation (GJM67+200). RNA was extracted using Trizol (Invitrogen, Carlsbad, CA) standard procedures and heat shock treatment (20 min at 65°C followed by 60 min at −80°C) for cellular disruption. Total RNA (250 ng) was reverse transcribed using a SuperScript III Platinum Two-Step qRT-PCR kit with SYBR green from Invitrogen. One microliter of the reverse-transcribed RNA was used as a template to amplify the genes, using primers to the ATG8 gene (sense, 5′-GAAGGCGGAGTCGGAGAGG-3′; antisense, 5′-GGCAGACATCAACGCCGC-3′) and to the ACT1 gene (sense, 5′-CACCAACTGGGACGATATGG-3′; antisense, 5′-GAAGGCTGGAACGTTGAAAG-3′). The expression of the ATG8 gene was assessed by real-time reverse transcription-PCR (RT-PCR) in a DNA Engine Opticon CFX98 C1000 Thermocycler (Bio-Rad). Results obtained were normalized to the reference gene ACT1. The data obtained were analyzed by applying the Livak method or the 2−ΔΔCT method (32) in order to verify the effect of nitrogen on expression levels, as follows: ΔΔCT = (CT(target gene) − CT(reference gene))test − (CT(target gene) − CT(reference gene))calibrator, where CT is threshold cycle.
Data are reported as mean values of at least three independent assays and presented as means ± standard deviations (SD). The arithmetic means are given with standard deviations with 95% confidence value. Statistical analyses were carried out using a Student's t test or two-way analysis of variance (ANOVA). P values of less than 0.05 were considered statistically significant and are marked at the tops of the columns or the curve points in the figures.
During wine fermentation, yeast cells are exposed to environmental stresses such as nitrogen depletion that can negatively affect cell growth and cell viability and ultimately lead to fermentation arrest. In the present work, the effects of nitrogen limitation during fermentation were mimicked using the wine yeast strain S. cerevisiae PYCC4072 in a grape must synthetic medium with high and low nitrogen contents under anaerobiosis. Nitrogen depletion effects on cell density, viability, and fermentation activity were assessed (Fig. (Fig.1).1). Nitrogen limitation (GJM67) resulted in lower fermentative activity, as reflected by the high glucose concentrations and low CO2 levels present in the medium at 168 h compared to the control fermentation (GJM267), where glucose was completely fermented (Fig. 1C and D). Consistently, CFU analysis showed that under nitrogen limitation conditions (GJM67), the number of CFU from 48 h on is significantly lower than that of the control fermentation, reflecting the early arrest of yeast cell division (Fig. 1A and B). After nitrogen refeeding at 72 h, yeast growth was reinitiated, and fermentation (measured as glucose consumption and CO2 production) was completed with a delay of 24 to 48 h compared to the control fermentation (Fig. (Fig.1).1). These results are in agreement with the previous characterization of fermentation profiles applying similar nitrogen conditions although under a microaerophilic environment (22, 23).
Different physiological parameters were then assessed with the aim of gaining new insights on the mechanisms underlying the low fermentative activity observed under the above described nitrogen conditions.
Intracellular ROS accumulation has been described as the result of stress promoted by different nutritional and environmental factors that affect grape must fermentation (4, 12, 17, 26). To further characterize the mechanisms underlying the slow fermentation observed under the above described nitrogen-limiting conditions (Fig. (Fig.1),1), the levels of intracellular ROS were periodically evaluated by flow cytometry using dihydroethidium (DHE) and MitoTracker Red CM-H2XRos (MitoTracker) as probes, respectively, sensitive to superoxide anion and overall cellular ROS (Fig. (Fig.2).2). According to data previously reported for hypoxic fermentation in high glucose-containing medium (17), it was observed that ROS are accumulated in 10 to 20% of cells undergoing the control fermentation, as revealed by both probes (MitoTracker and DHE) (Fig. (Fig.2).2). Nitrogen depletion resulted in an increased percentage of cells with ROS accumulation (40 to 50%) when ROS were evaluated by MitoTracker Red CM-H2XRos (Fig. (Fig.2A)2A) but not by DHE (Fig. (Fig.2B),2B), excluding the time point 24 h, when there are no major differences between the control and N-starved fermentations in what concerns the fermentation profiles, and at 72 h, when nitrogen depletion seriously compromised growth (Fig. (Fig.1).1). These results, although indicating that nitrogen depletion led to ROS accumulation, suggest that superoxide anion was a minor contributor to the overall ROS as determined by MitoTracker Red CM-H2XRos. Nitrogen refeeding at 72 h resulted in a significant decrease in the percentage of cells with high ROS content compared to nitrogen-limiting conditions; the final ROS levels in refed cells were in fact much lower than those found in the control fermentation at the later time points (Fig. (Fig.2).2). Similar to results under nitrogen-limiting conditions, superoxide anion was not a major contributor to the overall ROS in nitrogen-refed fermentation (Fig. (Fig.2).2). To evaluate the relevance of mitochondrial membrane potential alterations on the generation of superoxide anions, cells were stained with rhodamine 123 (Rh123). The current results showed that nitrogen-limiting conditions lead to a decrease of mitochondrial membrane potential throughout fermentation, as represented for 96 h (Fig. (Fig.2C).2C). Thus, under nitrogen-limiting conditions, it can be hypothesized that lower mitochondrial membrane potential could account for the lower levels of superoxide anions detected. However, it is also necessary to consider that superoxide dismutase (SOD) activities are increased as well (Fig. (Fig.33).
The cellular responses to ROS include the induction of enzymatic antioxidant defense systems such as catalase (CAT) and superoxide dismutase to maintain physiological homeostasis. To evaluate the role of antioxidant systems on ROS accumulation promoted by nitrogen-limiting conditions, activity measurements were made for superoxide dismutases (Sod1p, which is Cu/Zn dependent, and Sod2p, which is mitochondrial and Mn-dependent) and catalases (Cta1p, which is peroxisomal, and Ctt1p, which is cytosolic), both classes of enzymes playing essential roles in protecting yeast cells against oxidative stress (15). Globally, cells from nitrogen-limiting conditions displayed Sod1p, Sod2p, and Cta1p activities higher than those observed in the control fermentation (Fig. (Fig.3).3). Nevertheless, Sod2p and Cta1p displayed an increased activity over time compared to Sod1p activity (Fig. (Fig.3).3). These results are in accordance with previous reports showing that ROS, particularly sublethal H2O2 concentrations, induce the transcription of SOD1 and SOD2 genes but with a more robust induction of Sod2p activity (11, 13, 24). Moreover, the increased Sod2p activity is most probably responsible for the lower levels of superoxide anion and their irrelevant contribution to overall ROS observed under N-starved fermentation. Nitrogen refeeding resulted in a global kinetic enzymatic activity pattern similar to that found for nitrogen-limiting fermentation (Fig. (Fig.3)3) with a slight decrease over time, probably reflecting the observed cell growth (Fig. (Fig.1).1). Consistent with anaerobic conditions and the regulation of Ctt1p by oxygen (6), no activity of this enzyme was detected at any of the time points analyzed.
Overall, nitrogen-limiting fermentations under either anaerobic (Fig. (Fig.3)3) or microaerophilic conditions (from previous in silico data of the genome-wide expression analysis in S. cerevisiae PYCC4072 ; accession number GSE5842 [www.ncbi.nlm.nih.gov/geo/]) suggest that the antioxidant enzymatic defense systems, superoxide dismutase and catalase, are triggered to counteract the increased ROS generation (Fig. (Fig.2).2). The current findings further indicate a major role of Sod2p and Cta1p in nitrogen limitation under anaerobic conditions.
The results presented above demonstrate that in spite of the increased Sod2p and Cta1p activities, nitrogen limitation affects fermentation performance by causing increased ROS accumulation. To evaluate whether ROS accumulation was accompanied by damage to cell structures, the ability of plasma membrane to effectively exclude propidium iodide (PI) was assessed by flow cytometry. Plasma membrane integrity of cells from the control fermentation was mainly unaffected while under nitrogen-limiting conditions the percentage of PI-positive cells significantly increased from early time points (Fig. (Fig.4).4). After nitrogen refeeding, the number of cells with loss of plasma membrane integrity was significantly decreased (Fig. (Fig.4).4). Altogether, the results showed that nitrogen-limiting conditions induce loss of plasma membrane integrity, which is directly correlated with increased ROS accumulation and with lower survival than in the control and refed fermentations (Fig. (Fig.11 and and4),4), promoting the link between the two phenomena, ROS accumulation and plasma membrane injury.
To further understand the impact of ROS accumulation induced by nitrogen-limiting conditions, cell cycle progression was analyzed. In comparison to the control fermentation, nitrogen-limiting conditions promoted a persistent cell cycle arrest in phases G0/G1 at 48 h when nitrogen was completely exhausted from the medium (22, 23) (Fig. (Fig.5B).5B). Nitrogen depletion-mediated G0/G1 cell cycle arrest is a well-known phenomenon that seems to be modulated by downregulation of the G1 cyclin CLN3 (10, 22). Nitrogen refeeding at 72 h allowed cells to reenter cell cycle (Fig. (Fig.5C),5C), as reflected by cell density and viability (Fig. (Fig.1)1) and reduction of ROS accumulation (Fig. (Fig.22).
Overall the results suggest a close relationship between increased ROS accumulation and negative effects on cell membrane integrity and cell cycle. Both results obtained under nitrogen-limiting conditions and also after nitrogen refeeding support this hypothesis.
Autophagy is known as a multifunctional mechanism associated with different physiological and cellular processes in eukaryotes. A key regulatory component of autophagy is Atg8p, a ubiquitin-like protein that acts in the vesicle enlargement step, playing an important role in the cross talk between autophagy and other cellular and physiological networks (7, 27). To assess whether the yeast cells could respond to nitrogen limitation by inducing autophagy, mRNA levels of the widely known marker gene ATG8 were monitored by RT-PCR in yeast cells collected under the different fermentation conditions (Fig. (Fig.66).
ATG8 gene expression was observed at relatively low levels and increased during the time course under all the fermentation conditions, reflecting either carbon or nitrogen limitation (Fig. (Fig.6).6). Nonetheless, the highest expression levels of the ATG8 gene were observed under nitrogen-limiting conditions at 168 h (Fig. (Fig.6),6), corroborating previous studies where autophagy is essential to allow yeast survival in the absence of nitrogen (33). Furthermore, these data also support the theory that autophagy triggered by nitrogen limitation is not sufficient to evade slow fermentation (Fig. (Fig.1).1). Conversely, nitrogen refeeding induced a decrease in the mRNA levels of the ATG8 gene throughout fermentation, indicating that the nitrogen supply overcomes cellular needs of the autophagic process. Overall, and of utmost relevance, triggering autophagy under nitrogen-limiting fermentation is not sufficient to prevent low fermentation activity.
Our previous study revealed that nitrogen limitation induces several different transcriptional alterations compatible with a yeast general stress response (22). The data presented herein obtained with a complementary physiological approach is the natural next step that gives further insights on the cellular effects of nitrogen limitation under conditions mimicking wine fermentation. Of relevance, nitrogen limitation triggers ROS accumulation, without involvement of the superoxide anion, and this is associated with increased activity of some antioxidant enzymes, particularly, Sod2p. These phenomena are associated with increased loss of plasma membrane integrity, cell cycle arrest at G0/G1 phases, and induction of autophagy. Importantly, the induction of autophagy under nitrogen-limiting conditions is not sufficient to provide an identical control fermentation profile. In fact, fermentation reestablishment was observed only when nitrogen was added into the nitrogen-limiting fermentation, and the overall negative cellular effects were completely reverted. From an applied point of view, the present work further emphasizes the relevance of nitrogen availability during grape must fermentation and reveals that both ROS accumulation, measured by MitoTracker, and loss of plasma membrane integrity, but not ATG8 expression, could be useful as markers for early detection of nitrogen limitation in wine fermentations.
This work was supported by projects PTDC/AGR-ALI/71460/2006 and PTDC/AGR-ALI/68284/2006 of the Fundação para a Ciência e Tecnologia. C.B. was a recipient of a Ph.D. grant from FCT (SFRH/BD/61881/2009).
We thank R. N. Bennett for English revision of the manuscript.
Published ahead of print on 15 October 2010.

domain: biology


text:
27th January 2014
People coming up to retirement before April 2016 may be offered the chance to buy additional units that could upgrade their state pension by as much as £25 a week. The pensions minister Steve Webb has been promoting the planned introduction of a scheme to allow people to buy additional amounts of state pension, as reported in today’s Daily Mail.
The plan envisages pensioners being able to pay in between £900 to £22,500 or perhaps more to top up their state pension. Their weekly state pension will then increase by a £1 up to a cap of £25 for every £900 paid. The minister says the scheme is likely to benefit the self employed and women the most.
People will, of course, have to find the cash from somewhere but it is likely that buying a top up to the state pension should prove more generous that buying an annuity on the open market though of course those due to retire will have to examine the full details of the scheme to test the comparison.
Preliminary calculations by Hargreaves Lansdown head of pension research Tom McPhail suggest that it will represent a better deal than the average annuity.
In a note about the proposals, he says: “Reports today suggest that an extra £1 a week of class 3A state pension would cost £900 to buy. On the open market, an inflation linked single life annuity for a 65 year old currently costs £1,468 for each £1 a week of income. This suggests that the government may also be offering this new scheme on very generous terms”.
The scheme is different to the existing arrangement where pensioners are able to fill gaps in their state pension entitlement. Hargreaves Lansdown has also compared the two systems below and made some initial calculations around what the current scheme, the new scheme and indeed annuity rates too.
Hargreaves Lansdown’s calculations
Current scheme – buying Class 3 NI
Voluntary national Insurance contributions are paid by those who do not have sufficient qualifying years to qualify for the full basic state pension. They also count towards Widowed Parent’s Allowance, Bereavement Payment and Bereavement Allowance.
Voluntary National Insurance contributions normally have to be paid before the end of the sixth tax year following the tax year to which the contribution relates. The rates for Voluntary National Insurance contributions may be higher if the contribution is paid more than two tax years after the tax year to which it relates, potentially at the highest rate applying since that tax year.
New scheme – buying Class 3A NI (not yet introduced but flagged in the media today)
Those who reach state pension age before 6 April 2016 with an entitlement to a UK state pension will be able to make voluntary class 3A national insurance contributions from October 2015. This facility will be available for a limited time, potentially less than two years.
Each class 3A voluntary contributions will increase entitlement to additional state pension, not basic state pension, by £1 a week. It is expected that the increase will be capped at £25 a week. The extra amount of additional state pension will be paid in the week following the day on which the additional contribution is paid.
Previously the government has claimed that this scheme will be set at an ‘actuarially’ fair rate based on the latest gender neutral life expectancy figures available to the ONS. The cost will vary according to age; the older the age the lower the price. Today’s reports suggest that this may not in fact be the case.
As class 3A voluntary contributions are both designed for those over state pension age and with a value which is dependent on life expectancy, the government will consider whether there should be a cooling off period.
McPhail adds: “The government is looking at some very interesting ideas to help pensioners to boost their state pension entitlement. Previously the government had been looking to limit any additional state liability to the risk of pensioners living too long (and therefore costing the state more money). These ideas suggest that there is a bit of a rethink going on, with the government willing to offer more generous state pension foundations, on top of which private pension savings can then be built. In principle the terms offered by the government for these additional state pension deals look very attractive.”
Watch more Mindful Money TV: The state retirement age rise and how to plan for it

domain: finance


text:
3.5 years old patient with DCD and Developmental Delay.
1. The PT asks the patient to step over an obstacle and climb the stairs without using the handrails. The patient tries, but when she steps over the obstacle she leans on her left hand.
2. The patient tries not to use the handrails when climbing the stairs, although the task is difficult. The challenge leads her to fixate both hands. She leads with her right leg in a 'step to' technique, but loses balance on the second step and leans right.
3. Because it is harder to go down the stairs, the patient uses both hands. She uses a 'step to' technique, leading with her left leg. The same technique is used also when crossing over the obstacle.
A Special thanks to the "Ha'gan Ha'shikumi" and their amazing staff, for sharing their knowledge, thoughts and experience.
Video Guide 1


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In the vast landscape of design and aesthetics, one element stands out quietly yet powerfully—white boxes. These unassuming entities, often overlooked in the whirlwind of colours and patterns, possess white boxes a unique charm that transcends their simplicity. In this article, we embark on a journey to unravel the mystery of white boxes, exploring their multifaceted roles and the impact they have on our visual experiences.
Painting with Minimalism
In a world saturated with visual noise, white boxes emerge as champions of minimalism. Like a blank canvas waiting for an artist’s brush stroke, these unadorned spaces provide a clean, serene backdrop, allowing other elements to shine. It’s the art of saying more with less, a visual poetry that captures attention by not clamouring for it.
Calming the Chaos
Delving into the psychology of colour, white exudes tranquillity and simplicity. White boxes serve as visual sanctuaries, offering a moment of respite in a chaotic digital realm. Research suggests that exposure to white spaces can lower stress levels, providing users with a sense of calm and clarity.
The Blank Slate Effect
White boxes also trigger the “blank slate” effect, inviting users to project their thoughts and emotions onto the pristine canvas. In a digital age dominated by clutter, this open invitation to interpretation fosters a deeper connection between content and audience.
The Functionality of White Boxes
White boxes are not mere visual accessories; they are functional powerhouses. In web design, they guide users, signalling importance or highlighting crucial information. A well-placed white box can be the difference between a seamless user experience and a confusing labyrinth.
Responsive Design Elegance
The versatility of white boxes shines in responsive design. These adaptable elements effortlessly adjust to various screen sizes, maintaining clarity and readability. They are the unsung heroes ensuring that your content looks impeccable, whether viewed on a desktop or a smartphone.
Optimising the Canvas
Utilising white boxes to optimise content involves a delicate balance. Overloading them with keywords risks compromising user experience, while too few may result in missed SEO opportunities. The key is a harmonious integration that aligns with both user needs and search engine algorithms.
Benefits of Incorporating White Boxes in Design:
Enhanced Readability and Focus
White boxes create a clear visual hierarchy, guiding the reader’s eye and ensuring a seamless reading experience.
Use them to encapsulate key information, drawing attention to critical details and improving overall content comprehension.
Clean and Professional Aesthetics
White boxes contribute to a polished and professional look, especially crucial in corporate websites or portfolios.
Employ them to frame images, text, or call-to-action buttons, adding a touch of sophistication to your design.
Improved User Engagement
The simplicity of white boxes minimises distractions, allowing users to focus on the content without being overwhelmed.
Use them strategically in layouts to encourage user interaction and increase overall engagement metrics.
Versatility in Branding
White boxes offer a neutral canvas that can adapt to various brand colours and styles.
Incorporate them as part of your brand identity, ensuring consistency across different platforms and marketing materials.
Responsive Design Adaptability
White boxes seamlessly adapt to different screen sizes and resolutions, ensuring a consistent user experience across devices.
Utilise them in responsive designs to maintain clarity and readability on both desktop and mobile platforms.
Identify your main strategically place it within white boxes where it enhances content relevance.
Avoid Stuffing; maintain a natural flow that benefits both users and search engines.
Use white boxes to highlight essential information, incorporating relevant keywords naturally.This not only improves user experience but also signals search engines about the importance of specific content.
Maintain a balanced keyword density within white boxes to optimise for search engines.
Regularly review and update content to align with evolving SEO algorithms and user search patterns.
Leverage white boxes for internal linking, directing users to other relevant pages within your website.
Include anchor text with appropriate keywords to strengthen the internal link structure.
Ensure that white boxes are designed to be mobile-friendly, adapting seamlessly to different screen sizes.
Optimise font sizes and spacing within these boxes for optimal readability on smaller screens.
As we navigate the ever-changing landscape of digital design and SEO, it’s crucial to stay informed and adapt. Consider the following forward-looking strategies:
Explore the potential of AI in optimising white box placement and content for enhanced user engagement.
AI algorithms can analyse user behaviour and suggest improvements for a more personalised experience.
Voice Search Optimization
With the rise of voice search, adapt your content within white boxes to align with conversational queries.
Incorporate long-tail keywords and natural language to enhance voice search compatibility.
Visual Search Integration
As visual search gains prominence, optimise images and visual content within white boxes for improved discoverability.Utilize descriptive alt text and relevant keywords to enhance visual search results.In conclusion, white boxes are not just design elements; they are dynamic tools that can significantly impact user experience and SEO. By leveraging their benefits strategically and staying attuned to emerging trends, you can ensure your content remains both visually appealing and discoverable in the evolving digital landscape.
In the ever-evolving landscape of design and digital experiences, white boxes emerge as timeless companions. They white boxes navigate the delicate balance between aesthetics and functionality, leaving an indelible mark on user experiences and SEO strategies alike. As we continue to explore the vast possibilities of visual storytelling, let’s not underestimate the quiet elegance of the white box—more than just a canvas, but a silent storyteller in the grand narrative of design.

domain: design


Regardless of your soil, using hard water longterm can cause problems. One of the most visible is the buildup of limestone deposits. You get them around the top of the pot, on the plant if you aren't careful, and out of sight they can clog up the soil. The deposits can be dissolved away using an acid but this is a slow process, perhaps best done by leaving the pot out in the rain for a few weeks (you'll goodness Britain sends all that acid rain!).
Hard water on an acid compost is a particularly bad combination. The acid soil, mainly the organic components, and the alkaline water will react and the result tends to be a nasty mess.
Hard water on an alkaline soil shouldn't cause any immediate problems, but may produce nutrient deficiencies. Certain nutrients are not available in alkaline conditions and one of the ways that plants get access to them is because slightly acid rainwater makes them available. There are other mechanisms involving acidic secretions from the roots and symbiosis with fungi and bacteria, but just remember nutrient deficiency of the plants start to look a big jaded. One nutrient that is always in short supply on alkaline soils is nitrogen, and limestone specialists are usually slow growing plants.


Strategically located near Września in the province of Wielkopolska investment area with an area of 18.36 ha, including the following plots:
- 42/13, 102/11, 103, 105/20, 106/7, 106/9 – industrial areas,
- 45, 46, 51, 104, 106 – railway areas,
- railway tracks and other communication areas.
- for the entire area there is a local spatial development plan adopted by RESOLUTION NO. XVIII/265/2016 OF THE SEPTEMBER CITY COUNCIL (click here to download the plan) and a building permit for a hall with an area of 65,000 sq m and a height of up to 40 m / 50 m (10 floors),
- area for production facilities, depots and warehouses,
- building area – 80%,
- the possibility of building a railway siding,
- area equipped with all utilities,
- proximity to the A2 motorway.
Price for 1 sqm: PLN 270.00 + VAT


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Hike by Jessica Taekman, originally published in the October 2010 issue of Bay Nature magazine
Even though the Bay Area has a tremendous variety of open spaces, many people find themselves drawn again and again to one particular park. For lots of folks in Marin, that's Terra Linda/Sleepy Hollow Ridge, northwest of San Rafael.
And with good reason. Terra Linda/Sleepy Hollow Ridge covers more than 1,100 acres with easy terrain for hikers, mountain bikers, dog walkers, and equestrians. You can get to the preserve's northern reaches from the gate at the end of Freitas Parkway. Here, a paved pathway marks where developers came close to building a thoroughfare across the ridge from Sleepy Hollow to Terra Linda. Luckily, community members stepped in to block the road's completion and the Marin County Open Space District eventually took ownership of the ridge. Now the only vehicles passing over the ridge are pedaled two-wheelers.
The paved bike path makes a brief uphill climb, then quickly gives way to a broad, rocky trail. Serpentine outcroppings dot the hillside. Mount Tam rises to the south, and from spots along the trail you'll glimpse Big Rock Ridge to the north and, to the east, the turquoise roof of the Marin Civic Center and the broad expanse of San Pablo Bay. The preserve's trails wind through open grassland and stands of coast live and valley oak, bay, and buckeye.
Getting there: From Highway 101 in Marin, exit at Freitas Parkway (#455). Drive west on Freitas about 1.2 miles to the open space gate.


Companies are facing a constant battle against criminals who are using ever more sophisticated techniques and technologies to commit their crimes. As the criminals get smarter, companies need to innovate. Increasingly, forward-thinking organizations are turning to technology to help detect fraud attempts, minimize losses, prevent further exposure, identify culprits, and gather evidence to help law enforcement catch and prosecute criminals.
IBM i2 Analyst’s Notebook delivers data storage, analysis, visualization and dissemination capabilities that enable government agencies and private sector businesses to increase the value of the massive amounts of information that they collect. These tools give organizations the ability to discover hidden connections and patterns in data. This insight helps identify, predict, prevent and disrupt criminal, terrorist and fraudulent activities.
Rapidly piece together disparate data into a single cohesive intelligence picture.
Identify key people events, connections and patterns with innovative features like social network analysis, “list most connected” and “find connected networks”.
Increase understanding of the structure, hierarchy and method of operation of criminal, terrorist and fraudulent networks.
Simplify the communication of complex data to enable timely and accurate operational decision making.
Capitalize on rapid deployment that delivers productivity gains quickly using a well-established visual analysis solution.
See connections and patterns
IBM i2 Analyst’s Notebook Connector for Esri delivers association, temporal and geospatial analysis by integrating with Esri’s ArcGIS Server geospatial functions.
Connect directly to data
i2 iBridge connects to select enterprise databases with search/query capabilities to return analysis-ready data. i2 Information Exchange for Analysis Search for Analyst’s Notebook connects to/searches multiple sources at once to retrieve analysis-ready data.
i2 Text Chart: Intuitive, user-controlled text extraction and visualization to help with unstructured data assimilation. i2 Text Chart Auto Mark: Desktop app that automatically discovers and highlights entities of interest within a wide variety of documents.
Analyze call records
i2 Pattern Tracer rapidly analyzes large volumes of call detail records to identify call clusters and uncover key participants. It can quickly identify potential call targets and help prevent future call incidents.
IBM i2 Chart Reader
Successful investigations often depend on the ability to disseminate. i2 Chart Reader allows analysts to share intelligence with those who don’t have access to i2 Analyst’s Notebook. Download it for free in the Resources section.Contact i3solutions today to learn more about IBM i2 Analyst’s Notebook and how to protect your organization from criminal, terrorist and fraudulent activities.


text:
ALBANY State Sen. Patricia A. Ritchie, R-Heuvelton, said she tentatively supports keeping the personal data of pistol permit carriers out of the public eye.
Right now if you were to call up you can find out if someone has a pistol permit, Mrs. Ritchie said.
Mrs. Ritchie said having the information available helps to provide a certain measure of public safety, but she doesnt think it should be broadcast by media outlets.
Im waiting to see what the leadership does, Mrs. Ritchie said.
Assemblywoman Addie J. Russell, D-Theresa, is introducing legislation later this week that will prevent the public from having access to the personal information of pistol permit carriers.
This comes after the Journal News, White Plains, published the names and addresses of all pistol permit carriers in Westchester and Rockland counties in December, including law enforcement agents.
Mrs. Russell is including the protection of pistol permit carriers in a larger bill that will increase the penalties for murdering a first responder.
Marks Law, named for a Cape Vincent emergency medical technician who was murdered while responding to an emergency call in 2009, was originally proposed by Mrs. Ritchie, but failed to pass the Assembly in 2012.
Mrs. Russell is including her new proposals in an updated version of Marks Law she hopes will gain the support of her chamber.


text:
BY Advocate.com Editors
January 05 2010 1:35 PM ET
Johnson & Johnson heiress Casey Johnson had been dead for several days when her body was discovered Monday morning.
Johnson, who was reportedly engaged to reality TV star Tila Tequila and was arrested in November for allegedly robbing her former girlfriend, had a history of drug use and erratic behavior.
Tequila told authorities she and Johnson had a fight on Monday, December 28, and when she tried contacting the heiress the following morning, her phone had been shut off.
Johnson and Tequila announced their engagement in a rambling video interview that made the rounds online.
“We're what you call U-Haul lesbians, where you meet and fall in love and move in the next day and have kids," Tequila shared on the video.
Days later, Johnson’s ex, Courtenay Semel — the daughter of former Yahoo! CEO Terry Semel — told E! Online’s Mark Malkin the engagement was a sham and just a ploy for attention.
The Los Angeles Police Department has opened an investigation into Johnson’s death. The heiress, who was known for tweeting multiple times per day, last posted to her Twitter account on December 29.
- WATCH: 'Christian' Family's Terrifying Response to Son Coming Out
- WATCH: Teen From Disturbing Video Explains How It Started
- MICHAEL SAM CUT FROM RAMS ROSTER
- WATCH: Dr. Drew Interviews Gay Teen Attacked by Christian Family
- Hot Sheet: Young, Cute, Dead
- Op-ed: This Is What Happens When a Generation Is Raised on LGBT Visibility

domain: celebrity


Two former party leaders and another two former charity ministers have written an open letter calling for emergency funding to help the charity sector survive the coronavirus crisis.
In a letter published in The Daily Telegraph newspaper, Ed Miliband, a former leader of the Labour Party who was also Minister for the Third Sector between 2006 and 2007, and Sir Iain Duncan Smith, former leader of the Conservative Party, said some charities were closing and thousands more were “teetering on the brink”.
The letter is also signed by two former ministers for civil society, Nick Hurd and Rob Wilson, who between them were in the post for seven years, plus the Rt Rev Stephen Cottrell, the Bishop of Chelmsford and General Lord Dannatt, chair of the National Emergencies Trust.
The letter highlights the work charities are doing to coordinate the response to Covid-19 and help vulnerable people and the NHS during the pandemic, but says income has collapsed.
The comments come after the launch of the #EveryDayCounts campaign by sector umbrella bodies to pressure the government into releasing funding to support charities during the pandemic amid concerns about lost fundraising income.
The National Council for Voluntary Organisations has estimated that at least £4.3bn could be lost in income to the charity sector if action is not taken to support charities during the pandemic.
The government has indicated it is working on a rescue package for the charity sector.
“Much of the crisis support does not help charities,” the letter says. “Many can’t access business interruption loans or the new business rate relief.
“Supporting wages for workers who can’t work won’t help charity trustees having to run big deficits to keep going. Many are already having to take decisions about redundancies and closures as the situation is financially so grave.
“The Chancellor has said the government is working on support for charities and, of course, it needs to be set up in the right way. It can’t come soon enough.
“Charities can’t help tackle coronavirus, or help us rebuild, if they no longer exist.”


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The Meyers-Briggs system is used to categorize people by personality types. The rarest personality is INFJ—it blends intuitiveness with judgmentalism and introversion. There are 15 traits that all INFJs possess. An INFJ:
1. Likes to Write
These people write a lot whether they maintain journals or author fiction. There’s often a preference for the spoken word over the written word, and likely because it lets them express their thoughts in a way that they cannot with others.
2. Tends to Overthink
This personality tends to epitomize paralysis by analysis and particularly in regard to social situations. Rather than let them play out, they run them over and over again in their minds.
3. Never Settles
INFJ-types think go big or home. They have a very all-or-nothing mentality, which means that they will often avoid roles if they feel that they cannot be the absolute best at them.
4. Struggles to Put Ideas to Words
For people with this personality, it’s not always easy to put the concepts in their heads into words. This is why they often struggle with face-to-face situations, which are often time-sensitive, and love writing, which usually isn’t.
5. Isolates Himself or Herself
Loners is what you might call these people, but they don’t necessarily see it as a negative. Whether they’re home, walking in park or enjoying coffee at a cafe alone, this is an opportunity to get their thoughts straight and find their inner selves.
6. Can Fit in Anywhere
INFJs are be-ers, which means they can fit in almost anywhere. No matter the crowd or the environment, they can adapt due to how flexible they are. While this may be construed as a negative, these people have no qualms with using it to their advantage.
7. Empathizes a Lot
Not everyone can empathize with others, but INFJs can do it so intensely that it really affects who they are. It generally means that they’re tremendously generous and compassionate even with strangers. They will rarely intentionally hurt someone.
8. Is Selective with Friends
It can be said that people with an INFJ personality are very selective and perhaps no more so than with those they select as friends. Since they are so comfortable alone, they would rather be isolated than be around people who made them feel not themselves.
9. Seeks Truth
Some people seek truth in all that they encounter. An INFJ is like that whether it be with the mysteries of the universe or their belief in God. They favor deep conversations and can often find depth in subjects most others may ignore.
10. Trusts Intuition
An INFJ has a strong sense of intuition, and perhaps more importantly, he or she will listen to it. Even if it steers them wrong, they believe it will be right more often than not.
11. Works Hard
There’s no such thing as a lazy INFJ. They give of themselves fully to the tasks at hand, and if they do not have a purpose currently, they’ll find one.
12. Is Forward-Thinking
A person with an INFJ mindset tends to see the big picture. She doesn’t get bogged down by trivialities, and he will always look ahead to what’s important in life.
12. Is a Problem Solver
They tend to be quite good at problem-solving particularly in real-world applications. If you face a logistical challenge, your INFJ friend may be just the person to sort it out.
13. Is a Walking Contradiction
An INFJ personality is made up of contrasting elements. The personality isn’t always in sync, and so they are prone to emotional changes and may even demonstrate bipolar-like behaviors.
14. Reads People Well
It can be said that an INFJ has an emotional intelligence with great depth. They’re not prone to manipulative people, and if words or actions are false, they’re quite good at spotting ulterior motives.

domain: psychology


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Dennis Lambert tried, he really tried to put his show business career as an award-winning songwriter aside.
The man who wrote the likes of "We Built this City," "Ain't No Woman," said "Baby Come Back," decided to give it a rest. He and his wife Bettina moved to Boca Raton in 2003 from New York to be closer to her mother, and he started selling high-end real estate. Now "the broker from Boca," as the national press corps dubbed him when Steve Carell was in talks to portray him in a movie, is not only writing but performing.
The kickoff of his national concert tour, "He wrote THAT?" is set for April 22 at Florida Atlantic University here, and will include guest artists that made some of his songs famous. They include Mickey Thomas, the lead singer of Starship ["We Built this City"], Peter Beckett, the lead singer of Player ["Baby Come Back"] and a member of the Little River Band.
"The concert is warm and fuzzy," he said. "I get to play some of my big hits and tell stories about what it was like to work with those artists. The audiences really respond."
Lambert's even a nominee for this year's Songwriters Hall of Fame. "I don't know if I'll get it," he said in an interview in his home, where some of his platinum and gold records decorate the wall.
It couldn't be a better comeback story for a man who produced at least 80 Top 100 Billboard Singles and was up for a Grammy 12 times. Then hung it up.
"My son Jody's documentary was the initial springboard for my resurgence," he said about the Spanish River High grad who filmed his father's performances in the Philippines in 2007, where Lambert amassed an Elvis-like following, according to press accounts.
That documentary, "Of All Things," got traction in 25 film festivals and led to a deal with Warner Bros. for a feature film with Carell, star of "The Office," in the lead.
"The showbiz bug was laying dormant in me," but he had never performed his own songs, Lambert said.
"Other people made the hits," his wife added.
But he accepted an invitation to perform in the Philippines, where he already had a cult following for his "Bags & Things" album in the '70s. And buoyed by that success, he has appeared since then at The Viper Room, Knitting Factory and Joe's Pub, and debuted his current show format at a benefit in Palm Springs, Calif. with other 10 performers including Natalie Cole and The Commodores.
Lambert is on a roll. He and his songwriting partner Brian Potter are shopping a Broadway show about the man who started the Harlem Globetrotters. "Typically it's $15 million or $20 million so it's hard to get the funding. You have to get lucky to get all the right people to say yes," he said.
Songwriting is hard work, Lambert said, and he was ready to retire. "I'd write them for weeks on end until we made a record, and then it's on to the next artist for 30 years," he said. "That was the story of my life." He estimates he has written 600 songs and has hundreds of commercial releases of his music.
He wants to set the record straight that stopping was a career choice. "I said, 'it's time to do something else.'"
He chose real estate because he knew the business through his investments. He works here for Lang Realty and has no intentions of stopping, despite his foray back into show business. "I like it," he said. "It's like the creative process, which is more analytical than people think."
"It's much more likely you can sell a house, than do a hit record," his wife added.