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Tattoo designs are some thing pretty much all of consider finding at a person place or a different. A standard tattoo that a lot of of us contemplate would be the tattoo. Just before you choose on obtaining a tattoo design, you can find various crucial things you will need to take into consideration and that might sway your opinion on whether it's ideal for you.
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All of these subjects may help you identify regardless of whether acquiring a tattoo design is the greatest decision for you.
Choosing a tattoo to the initially time can be quite a really hard issue to accomplish, particularly in the event the tattoo goes to become long-lasting with your entire body for that rest of your daily life.
For many, selecting to acquire a tattoo performed is actually a large amount a lot easier than picking out what tattoo design to own. Buying a style usually takes many endurance and time specifically for to start with timers who've tiny to no information about what types will search ideal on them. Arnold Santos Tattoo Artist Electric Tiger Tattoo San Diego Arnold Santos Tattoo Artist Electric Tiger Tattoo San Diego San Diego Tattoo Artists #6691
For the reason that a tattoo is often a lifelong financial investment, you absolutely need to make certain that you'll normally love the look that should be done on you.
Decide on your Tattoo design and desired location to start with.
This really is crucial that you decide. Most custom made tattoo designs are made to accommodate selected portions of the body and may be a bit distinct in men and ladies since of human body style versions. You'll be able to pay a visit to various tattoo studios and master from artists what styles may be ideal for your desired location. Most men have theirs around the chest and arm locations, which represent their masculinity, even though most ladies opt to have their tattoos within the lower back, nape and navel areas. In the event you even now can't decide on a design from a tattoo studio selection, you could look through tattoo sites, that have absolutely free personalized models. Nonetheless, should you by now have a very design but can't decide exactly where to really have it tattooed, you are able to talk to tattoo experts that can help you discover out where it can seem terrific on you. It's important that you simply choose time for you to identify where you believe it is best to put your tattoo since it usually takes a toll with your assurance particularly when deciding on a part in the overall body that exposes your tattoo. Look at factors like job restrictions, religious beliefs and cultural and social affinities. Tattoo Design Around Name
Your tattoo design mirrors your character.
Tattoos absolutely are a mirror of oneself. You are able to pick out a design that speaks about something that plenty of people will not find out about you, possibly something which is particular and that is hidden in you. As an example, numerous select a design that tells of an important event that has transpired of their lifetime. Some pick to acquire their kid's or partner's names tattooed on them too as information regarding other substantial activities which have created a terrific influence on their lives. Your tattoo design may well even become a image of the life-style, feelings and ideas you want to express in a innovative and inventive method.
Color Variable: A very important Factor to maintain in Mind
Picking a colour or possibly a mix of colours for your personal tattoo is vital. You may need to help keep it firmly in your mind that combining distinct colours can provide an extreme attract the overall completed overall look of your tattoo. But you also have to recall that tattoos do fade around time and that is why quite a few suggest choosing models with significantly less shade, but nonetheless with good attractiveness. In case you still want a exclusive and colourful sample, adjust to the re-coloring schedule so you can keep your tattoo's vivid shades.
Get in contact with fact: The Artist
So, with all your plans in mind and the sought after tattoo design available, it's the perfect time to get a tattoo artist that can make it easier to make your thoughts arrive into becoming, and 1 who can assist make your dream customized tattoo design tangible and precisely the a single you might have been searching for. Take time in finding the most effective tattoo artist there exists and be prepared to dish out some money much too as the most effective tattoo illustrators also are the best compensated ones.
You may additionally wish to consider viewing on-line tattoo designing contests where several excellent tattoo designers from all areas with the globe compete by showcasing their unique tattoo designs in return to get a prize. This way, you are doing not merely restrict your search for your great custom tattoo design for your speedy vicinity but you are also equipped to find fantastic talents throughout the world.
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A jolt to the sugar battle: AFL-CIO calls on American Crystal to return to "meaningful" negotiations with locked-out workers
The head of the AFL-CIO is pledging the full weight of more than 12 million members to stand in solidarity with sugar workers who've been locked out of their jobs at American Crystal Sugar plants for nearly a year. President Richard Trumka says keeping these 1300 workers from their jobs will do long-term damage not only to the workers and their families but the entire Red River Valley.

domain: news


Storing your winter clothes over Summer
Here's what I do.
First, lay your things out and have a close look at them, checking for any wear, pilling, holes or visible dirt/grime. You may or may not choose to repair – by simply replacing buttons or closures, darning or prettily embroidering around any holes, worn patches or unravelling seams and address the pilling in areas of high friction like under the arms, cuffs or back - by gently removing the pills.
I’d recommend either a wool comb or better yet, use a mains power electric (not battery) Clothes Shaver for a more gentle and consistent attention rather than just cutting off with scissors or blades and risking snipping into your hand knit or hand crochet.
The Classic 50 Clothes Shaver can be used on your family’s clothes, suits, woven and even upholstery fabrics too. It’s the tool of choice for many dry cleaners and fabric care specialists and unlike, the small wool comb, it won't get lost in the knitting bag. In fact, why not keep it with your iron?
Next, I’d get set up to hand wash on a fine day – not too sunny or windy - and just lightly launder using a gentle Eucalan delicate wash which is biodegradable and safe – not a harsh stripping detergent. Available in 4 subtle fragrances and unscented too. It’s a non rinse solution which is a time and water saver but my recommendation is to measure it out with a teaspoon (1 tsp to 4 litres of lukewarm water or less if you’re just washing a pair of socks or small item) into a basin.
To wash, just press the item below the water until it absorbs all the water. Gently squeeze the item to release any dirt and you can leave in that water for no longer than 10-15 minutes - about a cup of tea's duration - then remove from the water, drain the bulk of the water - I sit in a clean colander for a few minutes). Remove excess water by rolling in a clean towel and laying flat in the shade – ensure your garment lays in the shape it’s meant to and just allow to dry naturally.
Store in your preferred way. I'd avoid folding in half along the centre fronts and back to prevent permanent creases. If you want an expert tutorial on folding, why not see Marie Kondo and her special way of wrangling clothes. Link to her YouTube video here.
There are special purpose zipped or vacuum sealed bags that prevent insects getting into the fabrics. I don't use chemicals near the actual garments but have found that those moth traps kept in the wardrobe area help me keep a check on insect activity in the hot months. There will be a lot more advice as to keeping those woollies bug-free on the internet. Martha Stewart has some advice on protecting against clothes moths here.
However, I do have some very special knitwear that is stored in sealed bags in my deep freezer so that "No way! Never!" will any moths venture near them. It’s kinda cool hearing the kids say “Mum, where’s the ice-cream? and why's your cardigan in the freezer!"


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Applications. Ketones are produced on massive scales in industry as
solvents, polymer precursors, and pharmaceuticals.
Ketone, any of a class of organic compounds characterized by the presence of a
carbonyl group in which the carbon atom is covalently bonded to an oxygen ...
Ketones are often used in perfumes and paints to stabilize the ... Other uses are
as solvents and intermediates in chemical industry.
Ketones and aldehydes can be ingredients in some familiar products. This lesson
will explore some of the uses of these organic molecules. It will...
www.ask.com/youtube?q=Uses of Ketones&v=IpNqSJrplQI
May 17, 2014 ... Uses of Aldehydes and Ketones. ... Aldehydes and ketones preparation -10-
Special Methods of Preparation - Duration: 9:32. Bangalore ...
www.ask.com/youtube?q=Uses of Ketones&v=SxRwBHEzzzU
Jun 24, 2015 ... Class 12: Chemistry: Aldehydes, Ketones and Carboxylic acids-II: Uses of
Aldehydes and Ketones.
Sep 24, 2015 ... Ketones are the major products from Chemical Industry. Looking into the uses
and need of ketones there listed out many chemical, medicinal, ...
Formal names for ketones include the prefix from the alkyl group and the suffix -
one. Two of the simplest are propanone, marketed under the name acetone, and
Mar 27, 2016 ... WebMD explains the uses and potential side effects of raspberry ketone
supplements, which some claim can be helpful for weight loss.
Find patient medical information for RASPBERRY KETONE on WebMD including
its uses, effectiveness, side effects and safety, interactions, user ratings and ...

domain: chemistry


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Rather than fighting the crowds at stores or spending hours online looking for the best prices this season, why don't you try a more time efficient and economical process that adds more personalization? This can be made possible by the one-stop shopping of a photo scanning service and the gift of memories.
Now there are many great DIY photo gifts, but calendars are perfect presents for almost anyone. By using a photo scanning service, you’ll gain access to everything needed to create calendars better than any mass-produced ones.
In addition to the great software and websites out there, many unique DIY photograph calendars are possible thanks to benefits, options, and resources gained using a photo scanning service, such as:
IDF – Intelligent Digital Finishing: Uses technology that enhances color, contrast, sharpness, and reduces noise for the best images possible. This is great for extremely old prints and slides and is included with FotoBridge photo scanning service packages.
Text note archiving: Scans backs of photos for dates, notes, or marks, available as an add-on to any scanning package. Extremely useful for aged photographs.
5 Unique DIY Photo Calendars You Can Create With The Help of a Photo Scanning Service
Using a photo scanning service allows you to get super creative with DIY photo calendars. Here are some of the most unique designs we found.
A timeless DIY photo calendar made easy thanks to DIY drag-and-drop style templates and layouts, or even word processor program calendar pages. It’s as easy as picking, printing, and cutting photos to size, attaching them to a month, and binding everything together!
This fun, reusable photo calendar also doubles as wall art! There are a few variations for this one, but for personalization, choose 31 personal photos for days of the month, seven for days of the week, and six for gaps.
With photo editing software, attach one number (1-31) to each photo and letters or words for days of the week on the seven “week” photos, print on 4x6 cardstock and/or laminate, clip together with FotoClips, hang, and rearrange each month!
This photo calendar is super unique and reusable, requiring 31-38 photos, magnetic tape, small envelopes (like coin envelopes,) and a label-maker to create dates with.
Print and cut photos into squares, cut envelopes in half to create pockets, glue them to the photos’ backs, apply magnetic strips, label each with a number (and 7 with days of the week,) and apply to any magnetic surface. Place notes or whatever you’d like into the pockets and re-arrange as needed.
Put all those antique CD cases to good use to make stand-up photo desk calendars. Find an online one-month calendar template, and after creating and printing each month’s page to fit the size of a CD case, slide it into the inside of the CD cover, prop it up, and continue switching out the month’s page. Take it a step further and insert a CD or DVD of photo albums or photo collections.
Perfect for the workaholic in your life, all you’ll need is a 3-ring binder easel, binder sheet protectors, photos, printed monthly calendar inserts, and a dry erase marker. After inserting the photos and calendar inserts into the sheet protectors, simply fill in the calendar and the user can make notes, re-do months, and use forever. Get a little creative and print photos and calendar pages on the same pages, adding borders, month names in fun fonts, and more!
The Gift that Keeps on Giving
Digitized photos aren't just perfect for the holiday season but the whole year round! We've compiled 25 great ways to share your photos once they go digital. Discover them all and share your memories with all of your friends and family.

domain: design


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Spring is asparagus season. The tender green spears appear almost magically from bare ground in many places in the Inland Northwest, and not all of them are in backyard gardens. To those in the know, there are wild patches of asparagus around old farms and homesteads, in empty fields and moist ditches. There is reportedly a patch somewhere on the old Fort Wright grounds where the military cooks grew vegetables for the soldiers in the early 1900s.
Growing asparagus is not difficult, but it takes a lot of garden space, some work to plant it and patience for it to grow to harvest age.
Asparagus grows from a root called a crown. The crown has multiple long fleshy rootlets topped with a growing tip. The crowns are available in the early spring in garden centers and online. It needs full sun and a well-drained sandy loam soil. A planting of asparagus can produce for 15 years or more, so it should be planted at the edge of a garden area or in its own separate bed where it can grow undisturbed.
Select large, plump roots with a good growing tip. Each root will produce about a half a pound of spears when it’s mature, so buy enough roots to satisfy the cravings in your household. Dig a trench 8 to 10 inches deep and fill the bottom with a couple of inches of good-quality compost or manure. Set the crown out 18 inches apart and cover with 2 inches of soil. Wait until the crowns send up a few shoots and then fill in the rest of the trench. Mulch around the new shoots with an inch of shredded pine needles, leaves or bark to keep the weeds at bay. Asparagus foliage is fernlike and can grow to about 3 feet tall.
An asparagus planting takes about four years to fully mature. A 1-year-old planting can be lightly harvested once. In the second year, harvest for about two weeks. In the third year, about three weeks. Starting in the fourth year, a bed can be harvested until the spears start to get smaller. These smaller spears will grow into the year’s fronds and replenish the roots. To harvest asparagus, slip a sharp knife into the soil at the base of a spear and gently cut it off.
Asparagus doesn’t like competing with weeds, so keep your planting well weeded and mulched. Fertilize them in the very early spring with a 10-10-10 fertilizer. Asparagus is moderately drought-tolerant but will benefit from deep soakings during July and August. Remember, its roots are at least 6 inches deep, so the water must go deep. In the fall, the fronds can be cut down or left until spring for winter interest.
There are dozens of varieties available to grow, but some of the best known include Mary Washington, Jersey Giant and Jersey Night. Purple varieties such as Purple Pacific and Purple Passion are also becoming popular. They have smaller yields but larger spears.
Pat Munts has gardened in Spokane Valley for over 35 years. She is co-author of “Northwest Gardener’s Handbook” with Susan Mulvihill. She can be reached at pat@ inlandnwgardening.com.
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domain: agronomy


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The Relationship Between Local Procurement Strategies of Mining Companies and Their Regulatory Environments: A Comparison of South Africa and Namibia
Published: February 2017
This project examines the local procurement regulatory frameworks of South Africa and Namibia and the extent to which the local procurement strategies of the mining companies that operate within those countries align with and are affected by these frameworks. Research also identified common factors that influence mining companies as they create local procurement strategies.
Read the report here.
Published: January 2017
This study explores the nature and root causes of mining related conflict. A more systematic understanding of conflict can help all stakeholders to apply preventive rather than reactive measures, and to improve approaches to conflict prevention, management, mitigation and transformation. The study was authored by a team of specialists in this field: Tony Andrews, Bernarda Elizalde, Philippe LeBillon, Chang Hoon Oh, David Reyes and Ian Thomson.
Read the report here.
CIRDI Strategic Plan
Published: September 2016
In the spring of 2016 the CIRDI team began a strategic planning process to lay the foundation for the future growth of the Institute. We sought input to our early developmental draft and are grateful to everyone who responded. Their suggestions and advice helped to strengthen our final plan, which is an important guiding document for our daily work and for building the Institute’s long-term sustainability.
Read the CIRDI Strategic Plan 2016-2021 September 2016
Better Lives Through Sustainable Natural Resource Governance: CIRDI’s response to Canada’s International Assistance Review
Published: August 2016
In May 2016, Global Affairs Canada launched a review and public consultations process focused on renewing Canada’s international assistance policy, programming and funding framework. CIRDI submitted a response, Better Lives Through Sustainable Natural Resource Governance.
For our three recommendations and to see the full report.
1. Recognize the role of sustainable natural resource management and governance as a vital opportunity for developing countries to lift their citizens out of poverty, and share Canadian expertise and experience to help make this happen.
2.Capitalize on the deep and broad expertise in Canadian universities and post-secondary institutions to strengthen Canada’s resource governance support to developing countries.
3.Promote innovative approaches to development based on real partnerships, the generation and sharing of foundational knowledge and leading-edge research.
Responding to the commodity bust: Downturns, policies and poverty in extractive sector dependent countries
Published: December 2015
The decline in prices experienced by most extractive commodities since 2011 has strained the economy and public finances of most resource-dependent countries, with implications for poverty reduction. This paper reviews recent studies on commodity cycles and their impacts on the poor in resource exporting countries. It assesses the relative vulnerability of extractive sector dependent countries to the current downturn and discusses policy options to reduce this vulnerability, including through countercyclical and extractive sector specific policies.
Extractive industries and poverty: A review of recent findings and linkage mechanisms
Published: December 2014
This paper surveys 52 empirical studies on relationships between extractive industries and poverty, addressing both poverty impacts and possible linkage mechanisms. Distinguishing these studies by type of resource extraction, the authors find industrial mining to be more frequently associated with poverty exacerbation and artisanal mining with poverty reduction. A review of 13 specific linkages between extractive industries and poverty highlights the importance of governance institutions and the limited effects of corporate social responsibility activities.
Reporting to Global Affairs Canada
Year End Report 2015-2016
In compliance with our Contribution Agreement with Global Affairs Canada (formerly DFATD) CIRDI submits an annual report that provides performance information for the last twelve months. Here is our Year End Report with appendices A-D and E-H.
Annual Work Plan
This Annual Work Plan, submitted to Global Affairs Canada, outlines CIRDI’s project work and activities for fiscal year 2016-2017.

domain: literature


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Vimeo partnered with ArtPrize and put out the call for people to shoot interviews, time-lapses, events, and the wonderful people of Grand Rapids.
Vimeo compiled the submitted footage and edited together a short crowd sourced documentary about The Art of Inspiration.
We were proud to have 98 individuals join our project and 56 videos submitted in total. Check out all of the videos submitted here: ArtPrize 2011
Thanks to all who submitted footage and provided interviews!
Footage submitted by:
Nicholas Kuiper, Anna Gustafson, SEI Studios, Aron Amond, Scopitone VidBox, Michelle Pittman, Karen Dunnam, Rapid Media, Hilary Cummins, Jonathan Brilliant, Carole Feuerman, Brent A. Saulic, Steven Soles, DVS Creative, Ryan Wyrick, Reed Multimedia, Kate Luttman, Jessica Matousek, Emily Gerlach, Jericho Castillo, Katie Fisher, Ryan Aeschliman, Elizabeth Shepard, Nick McVey, Casey DiDonato, Amber Andrews, Brian Hedrick, Kyle Hoogewarf, Thomas White, Christina Miller, James Soles, Elisabeth Haviland James, Lynda Ostosh, SUCH Video, Canvas Voice, Filippo Tagliati, Gretchen Vinnedge, River City Improv, Jason James, Kendall College of Art & Design, Grand Rapids Griffins / Occam Photography, John Vanderhaggen.
Kevin Buist, Filippo Tagliati, Mia Tavonatti, Ryan Spencer Reed, Mary Geeting, Deanna Morse, Paddy Johnson, Boshra al Saadi, Vyana Slattery, Soba One, Michael McCafrey.
The Little Village

domain: artistic


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Drag racing on a dragway just south of Bangor.
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Right now, residents of New Hampshire & Vermont can travel to Maine without providing a negative COVID-19 test result or quarantining.
Learn how our safe travel protocols are helping ensure everyone's visit is a safe one.

domain: geography


JOplin-area Catholic schools have added a new extracurricular activity that allows students to play video games as a competitive sport and challenge their rivals at other schools in the state.
St. Peter’s Middle School and McAuley Catholic High School launched a student esports program with the help of a $500 local community grant from Walmart Supercenter No. 79 in Joplin.
Esports, or electronic sports, is a term used to describe competitive multiplayer video games. In a school setting, esports competitions are organized and supervised by teachers.
Catholic school esports teams in the Joplin area meet on Tuesday and Thursday. Students play between two and five games per week against other teams. The season started in September.
Esports teams from Catholic schools in the Joplin area participate in the MOSEF conference. The Missouri Scholastic Esports Federation is a 501(c)(3) grassroots organization that supports middle and high school esports programs across the state.
Esports teams have been expanded to other Missouri schools in Mount Vernon, Branson, Capital City, Hollister and Reeds Spring. The Joplin School District also offers esports for junior and college varsity players.
Instead of traditional sports gear, the esports team members are outfitted with video game controllers where they play Super Smash Bros. Ultimate, a Nintendo fighting game where different characters compete for victory. They can select any Nintendo character, and many of them are from games the students grew up in.
Molly Bond, esports coach and English teacher at McAuley, said the program helps students who may not be on traditional sports teams to be part of a community.
“We saw a need in our student body, and some of our students didn’t have extracurricular activity,” she said. “It’s something that interested a lot of students. These are students who are not normally on sports teams, and this allows them to be part of a team where they can develop different skills. It gives them the discipline to come to practice after school and in order to have it, they have to keep their grades up.
Bond said regionals are right around the corner and it’s getting quite competitive between schools.
“We have everyone from beginners to experts, which is cool,” she said. “Our high school esports team is made up of all girls, and none of them had played Super Smash Bros. before that.”
Colleges and universities across the country offer financial aid and scholarships for students involved in esports. Competitive gaming is very much a spectator sport with an esports audience reaching 495 million in 2020, according to market research firm Newzoo.
Daniel Horinek, a 12-year-old sixth-grader at St. Peter’s Middle School, said he was introduced to new friends in the group and would like to see the esports program grow in Catholic schools. of the Joplin area. Six students are currently part of the college team.
“It’s fun to play against other schools because you never know how good they’re going to be,” he said. “But you can improve throughout the game.”
Nicholas Fajardo, a 12-year-old seventh-grader at St. Pete’s Middle School, said he excels at Super Smash Bros. and knew he would be a valuable addition to the esports team.
“I had some really good experiences through it,” he said. “It made school a lot more fun.”


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14.09.2012 in10:22 in Art -->
Jean-François Rauzier was born in 1952. Fascinated by photography from an early age, he graduated from the School Louis Lumière in 1976. He has since been working as a professional photographer, while developing a personal creative work.
In 2002 he invents the concept of the HYPERPHOTO and his artistic work takes an innovative and radical turn.


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Think you can win the 1st place at the snowman building contest?3.86 / 5.00 8,545 Views
Silly and cute VN. Part of The Somewhere Series.4.06 / 5.00 9,726 Views
How much you know about Famous Sculptures?3.95 / 5.00 10,150 Views
*For those complaining about the controls, please realize you're a tank :P We've reviewed the controls many times and this is as good as it gets. It takes some getting used to but this game is fun, enjoy :)
All about destructing tanks. Enemy panzers have broken loose, stop em in this overhead action-packed game. Features 2 fun modes!

domain: game


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Post-transcriptional modifications of rRNA and tRNA will be topics of Chapter 27 as their structure and function in protein synthesis will be a focal point. Thus, this section will focus on post-transcriptional modifications of mRNA.
Prokaryotic RNA Processing
Bacterial cells do not have extensive post-transcriptional modification of mRNA primarily because transcription and translation are coupled processes. Bacterial cells lack the physical barrier of a nucleus, which allows transcription and translation machineries to function at the same time, enabling the concurrent translation of an mRNA while it is being transcribed (Fig 26.2.1). Within this system the NusG protein plays a critical role. NusG has three separate domains and the functions of two of them are known. The NusG N-terminal domain (NusG-NTD) has the capacity to bind to RNAP, whereas the C-terminal domain (NusG-CTD) can combine with the NusE (RpsJ) component of ribosomes. These two functions of NusG enable transcription to be coupled with translation. NusG CTD can also bind to Rho to terminate transcription (Figure 26.2.1).
Fig. 26.2.1. The roles of NusG in transcription/translation coupling. (a) Composition of an active RNAP complex. RNAP is shown in dark grey, DNA in blue and nascent RNA in red. The ribosome is shown in green with the nascent polypeptide chain in light grey; the bulge in the small subunit denotes the location of NusE (RpsJ). NusG is shown in orange: its shape denotes two functional sections. The larger section denotes the N-terminal domain, which binds to RNAP. The smaller section denotes the C-terminal domain, which interacts with NusE in situ. Rho is shown in purple. (b) After translation is completed NusG remains bound to RNAP and may also bind to Rho through the C-terminal domain leading to termination of transcription.
Eukaryotic RNA Processing
In multicellular organisms almost every cell contains the same genome, yet complex spatial and temporal diversity is observed in gene transcripts. This is achieved through multiple levels of processing leading from gene to protein, of which RNA processing is an essential stage. Following transcription of a gene by RNA polymerases to produce a primary mRNA transcript, further processing is required to produce a stable and functional mature RNA product. This involves various processing steps including RNA cleavage at specific sites, intron removal, called splicing, which substantially increase the transcript repertoire, and the addition of a 5'CAP. Another crucial feature of the RNA processing of most genes is the generation of 3′ ends through an initial endonucleolytic cleavage, followed in most cases by the addition of a poly(A) tail, a process termed 3′ end cleavage and polyadenylation (CPA).
As seen in Section 10.4, polyadenylation is a required step for the correct termination of nearly all mRNA transcripts. With the exception of replication dependent histone genes, metazoan protein encoding mRNAs contain a uniform 3' end consisting of a stretch of adenosines. In addition to deterimining the correct transcript length at transcription termination, the poly(A) tail helps to ensure the translocation of the nascent RNA molecule from the nucleus to the cytoplasm, enhances translation efficiency, acts as a signal feature for RNA degradation, and thereby contributes to the production efficiency of a protein.
CPA is carried out by a multi-subunit 3′ end processing complex, which involves over 80 trans-acting proteins, comprised of four core protein subcomplexes (Figure 26.2.2 A). These consist of (1) cleavage and polyadenylation specificity factor (CPSF), comprised of proteins CPSF1-4, factor interacting with PAPOLA and CPSF1 (FIP1L1), and WD repeat domain 33 (WDR33) (shown in green on Figure 10.20 A); (2) cleavage stimulation factor (CstF), a trimer of CSTF1-3 (shown in red on Figure 26.2.2 A; (3) cleavage factor I (CFI), a tetramer of two small nudix hydrolase 21 (NUDT21) subunits, and two large subunits of CPSF7 and/or CPSF6 (shown in orange in Figure 26.2.2 A); and (4) cleavage factor II (CFII), composed of cleavage factor polyribonucleotide kinase subunit 1 (CLP1) and PCF11 cleavage and polyadenylation factor subunit (PCF11) (shown in yellow on Figure 26.2.2 A). Additional factors include symplekin, the poly(A) polymerase (PAP), and the nuclear poly(A) binding proteins such as poly(A) binding protein nuclear 1 (PABPN1).
CPA is initiated by this complex recognising specific cis-element sequences within the nascent pre-mRNA transcripts termed polyadenylation signals (PAS). The PAS sequence normally consists of either a canonical AATAAA hexamer, or a close variant usually differing by a single nucleotide (e.g., ATTAAA, TATAAA). It is located 10 to 35 nucleotides upstream of the cleavage site (CS) usually consisting a CA dinucleotide. The PAS is also determined by surrounding auxiliary elements, such as upstream U-rich elements (USE), or downstream U-rich and GU-rich elements and G-rich sequences (DSE).
As soon as the nascent RNA molecule emerges from RNA polymerase II (RNA Pol II), the CPSF complex is recruited to the PAS hexamer through numerous interactions. Upon successful assembly of this macromolecular machinery, CPSF3 performs the endonucleolytic cleavage followed by a non-templated addition of approximately 50-100 A residues.
Figure from: Nourse, J., et. al. (2020) Biomolecules 10(6):915
Alternative polyadenylation (APA) occurs when more than one PAS is present within a pre-mRNA and provides an additional level of complexity in CPA-mediated RNA processing (Figure 26.2.2 B). Early studies revealed a significant portion of genes undergo APA, and with the advent of next-generation RNA sequencing technologies the large scale regulation of genes has become apparent, with approximately 70% of the transcriptome exhibiting APA regulation. As APA determines 3′UTR content and thus the regulatory features available to the mRNA, changes in the APA profile of a gene can have enormous impacts on expression.
In eukaryotes, the 5′ cap, found on the 5′ end of an mRNA molecule, consists of a guanine nucleotide connected to mRNA via an unusual 5′ to 5′ triphosphate linkage (Fig. 26.2.3). This guanosine is methylated on the 7 position directly after capping in vivo by a methyltransferase. It is referred to as a 7-methylguanylate cap, abbreviated m7G.
In multicellular eukaryotes and some viruses, further modifications exist, including the methylation of the 2′ hydroxy-groups of the first 2 ribose sugars of the 5′ end of the mRNA. Cap-1 has a methylated 2′-hydroxy group on the first ribose sugar, while cap-2 has methylated 2′-hydroxy groups on the first two ribose sugars. The 5′ cap is chemically similar to the 3′ end of an RNA molecule (the 5′ carbon of the cap ribose is bonded, and the 3′-OH unbonded). This provides significant resistance to 5′ exonucleases.
snRNAs contain unique 5′-caps. Sm-class snRNAs are found with 5′-trimethylguanosine caps, while Lsm-class snRNAs are found with 5′-monomethylphosphate caps. In bacteria, and potentially also in higher organisms, some RNAs are capped with NAD+, NADH, or 3′-dephospho-coenzyme A. In all organisms, mRNA molecules can be decapped in a process known as messenger RNA decapping.
For capping with 7-methylguanylate, the capping enzyme complex (CEC) binds to RNA polymerase II before transcription starts. As soon as the 5′ end of the new transcript emerges from RNA polymerase II, the CEC carries out the capping process (this kind of mechanism ensures capping, as with polyadenylation). The enzymes for capping can only bind to RNA polymerase II that is engaging in mRNA transcription, ensuring specificity of the m7G cap almost entirely to mRNA.
Figure from: Brisbane
The 5′ cap has four main functions:
- Regulation of nuclear export
- Prevention of degradation by exonucleases
- Promotion of translation (see ribosome and translation)
- Promotion of 5′ proximal intron excision
In addition to the polyA tail, nuclear export of RNA is regulated by the cap binding complex (CBC), which binds to 7-methylguanylate-capped RNA (Fig 26.2.4). The CBC is then recognized by the nuclear pore complex and the mRNA exported. Once in the cytoplasm after the pioneer round of translation, the CBC is replaced by the translation factors eIF4E and eIF4G of the eIF4F complex. This complex is then recognized by other translation initiation machinery including the ribosome, aiding in translation efficiency.
Capping with 7-methylguanylate prevents 5′ degradation in two ways. First, degradation of the mRNA by 5′ exonucleases is prevented by functionally looking like a 3′ end. Second, the CBC and eIF4E/eIF4G block the access of decapping enzymes to the cap. This increases the half-life of the mRNA, essential in eukaryotes as the export and translation processes take significant time.
The mechanism that promotes the 5′ proximal intron excision during splicing is not well understood, but the 7-methylguanylate cap appears to loop around and interact with the spliceosome, potentially playing a role in the splicing process.
Decapping of a 7-methylguanylate-capped mRNA is catalyzed by the decapping complex made up of at least Dcp1 and Dcp2, which must compete with eIF4E to bind the cap. Thus the 7-methylguanylate cap is a marker of an actively translating mRNA and is used by cells to regulate mRNA half-lives in response to new stimuli. During the decay process, mRNAs may be sent to P-bodies. P-bodies are granular foci within the cytoplasm that contain high levels of exonuclease activity.
Eukaryotic genes that encode polypeptides are composed of coding sequences called exons (ex-on signifies that they are expressed) and intervening sequences called introns (int-ron denotes their intervening role). Transcribed RNA sequences corresponding to introns do not encode regions of the functional polypeptide and are removed from the pre-mRNA during processing. It is essential that all of the intron-encoded RNA sequences are completely and precisely removed from a pre-mRNA before protein synthesis so that the exon-encoded RNA sequences are properly joined together to code for a functional polypeptide. If the process errs by even a single nucleotide, the sequences of the rejoined exons would be shifted, and the resulting polypeptide would be nonfunctional. The process of removing intron-encoded RNA sequences and reconnecting those encoded by exons is called RNA splicing. Intron-encoded RNA sequences are removed from the pre-RNA while it is still in the nucleus. Although they are not translated, introns appear to have various functions, including gene regulation and mRNA transport. On completion of these modifications, the mature transcript, the mRNA that encodes a polypeptide, is transported out of the nucleus, destined for the cytoplasm for translation. Introns can be spliced out differently, resulting in various exons being included or excluded from the final mRNA product. This process is known as alternative splicing. The advantage of alternative splicing is that different types of mRNA transcripts can be generated, all derived from the same DNA sequence. In recent years, it has been shown that some archaea also have the ability to splice their pre-mRNA.
The splicing reaction is catalyzed by the spliceosome, a macromolecular complex formed by five small nuclear ribonucleoproteins (snRNPs), termed U1, U2, U4, U5, and U6, and approximately 200 proteins (Fig. 26.2.5). The assembly of the spliceosome on pre-mRNA includes the binding of U1 snRNP, U2 snRNP, the pre-formed U4/U6-U5 triple snRNP, and the Prp19 complex. This assembly occurs through the recognition of several sequence elements on the pre-mRNA that define the exon/intron boundaries, which include the 5′ and 3′ splice sites (SS), the associated 3′ sequences for intron excision, the polypyrimidine (Py) tract, and the branch point sequence (BPS). The assembly of the spliceosome during the process is depicted in Figure 10.23.
Figure from: Suñé-Pou, M., et. al. (2017) Genes 8(3):87
In mammals, the first catalytic step of the splicing reaction begins when the U1 snRNP binds the 5′ SS of the intron (defined by the consensus sequence AGGURAGU), and the splicing factors SF1 and U2AF cooperatively recognize the BPS, Py, and 3′ SS to assembled complex E or the commitment complex (Figure 26.2.5). Subsequently, U2 snRNP and additional proteins are recruited to the pre-mRNA BPS to form the pre-spliceosome or complex A. The binding of the U4/U6-U5 tri-snRNP forms the pre-catalytic spliceosome or complex B. After RNA-RNA and RNA-protein rearrangements at the heart of the spliceosome, U1 and U4 are released to form the activated complex B or complex B* This complex is responsible for executing the first catalytic step, through which the phosphodiester bond at the 5′ SS of the intron is modified by the 2′-hydroxyl of an adenosine of the BPS to form a free 5′ exon and a branched intron (Fig. 26.2.6). The reaction of the 2'-hydroxyl from the branchpoint adenosine nucleotide is known as a transesterification reaction. During this process, additional rearrangements occur to generate the catalytic spliceosome or complex C (Fig. 26.2.5), which is responsible for catalyzing the second transesterification reaction leading to intron excision and exon–exon ligation (Fig. 26.2.6). The resulting intron structure is referred to as a lariat structure. After the second catalytic step, the U2, U5, and U6 snRNPs are released from the post-spliceosomal complex and recycled for additional rounds of splicing.
Alternative Splicing (AS) offers an additional mechanism for regulating protein production and function. AS options are determined by the expression of or exposure to in trans elements present within unique cellular locations and environments. Additional sequence elements within the mRNA, known as exonic and intronic splicing silencers or enhancers (ESS, ISS, ESE, and ISE, respectively), participate in the regulation of AS. Specific RNA-binding proteins, including heterogeneous nuclear ribonucleoproteins (hnRNPs) and serine/arginine-rich (SR) proteins, recognize these sequences to positively or negatively regulate AS (Figure 26.2.7). These regulators, together with an ever-increasing number of additional auxiliary factors, provide the basis for the specificity of this pre-mRNA processing event in different cellular locations within the body.
Figure from: Suñé-Pou, M., et. al. (2017) Genes 8(3):87
There are several different types of AS events, which can be classified into four main subgroups. The first type is exon skipping, which is the major AS event in higher eukaryotes. In this type of event, a cassette exon is removed from the pre-mRNA (Fig. 26.2.8 a). The second and third types are alternative 3′ and 5′ SS selection (Fig. 26.2.8 b & c). These types of AS events occur when the spliceosome recognizes two or more splice sites at one end of an exon. The fourth type is intron retention (Fig. 26.2.8 d), in which an intron remains in the mature mRNA transcript. This AS event is much more common in plants, fungi and protozoa than in vertebrates. Other events that affect the transcript isoform outcome include mutually exclusive exons (Fig. 26.2.8 e), alternative promoter usage (Fig. 26.2.8 f), and alternative polyadenylation (Fig. 26.2.8 g).
Figure from: Suñé-Pou, M., et. al. (2017) Genes 8(3):87<h210.6refs">26.2.2 References
- Parker, N., Schneegurt, M., Thi Tu, A-H., Lister, P., Forster, B.M. (2019) Microbiology. Openstax. Available at: https://opentextbc.ca/microbiologyopenstax/
- Palazzo, A., and Lee, E.S. (2015) Non-coding RNA: what is function and what is junk? Frontiers in Genetics 6:2 Available at: file:///C:/Users/flatt/AppData/Local/Temp/fgene-06-00002.pdf
- Wikipedia contributors. (2020, July 9). RNA. In Wikipedia, The Free Encyclopedia. Retrieved 15:30, August 6, 2020, from https://en.Wikipedia.org/w/index.php?title=RNA&oldid=966784317
- Burenina, O.Y., Oretskaya, T.S., and Kubareva, E.A. (2017) Non-Coding RNAs As Transcriptional Regulators in Eukaryotes. Acta Naturae 9(4):13-25. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5762824/
- Khatter, H., Vorlander, M.K., and Muller C.W. (2017) RNA polymerase I and III: similar yet unique. Current Opinion in Structural Biology 47:88-94. Available at: https://www.sciencedirect.com/science/article/pii/S0959440X17300313
- Wikipedia contributors. (2020, May 8). Sigma factor. In Wikipedia, The Free Encyclopedia. Retrieved 17:50, August 7, 2020, from https://en.Wikipedia.org/w/index.php?title=Sigma_factor&oldid=955570499
- Bae, B., Felkistov, A., Lass-Napiokowska, A., Landick, R., and Darst, S.A. (2015) Structure of a bacterial RNA polymerase holoenzyme open protomer complex. eLife 4:e08504. Available at: https://elifesciences.org/articles/08504
- Petrenko, N., Jin, Y., Dong, L., Wong, K.H., and Struhl, K. (2019) Requirements for RNA polymerase II preinitiation complex formation in vivo. eLife 8:e43654. Available at: https://elifesciences.org/articles/43654
- Gupta, K., Sari-Ak, D., Haffke, M., Trowitzsch, S., and Berger, I. (2016) Zooming in on transcription preinitiation. J Mol Biol. 428(12):2581-2591. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906157/
- Wikipedia contributors. (2020, April 17). TATA-binding protein. In Wikipedia, The Free Encyclopedia. Retrieved 14:54, August 8, 2020, from https://en.Wikipedia.org/w/index.php?title=TATA-binding_protein&oldid=951583592
- Patel, A.B., Greber, B.J., and Nogales, E. (2020) Recent insights into the structure of TFIID, its assembly, and its binding to core promoter. Curr Op Struct Bio 61:17-24. Available at: https://www.sciencedirect.com/science/article/pii/S0959440X19301113#fig0010
- Ruff, E.F., Record, Jr., M.T., Artsimovitch, I., (2015) Initial events in bacterial transcription initiation. Biomolecules 5(2):1035-1062. Available at: https://www.mdpi.com/2218-273X/5/2/1035/htm
- Kireeva, M., Opron, K., Seibold, S., Domecq, C., Cukier, R.I., Coulombe, B., Kashlev, M., and Burton, Z. (2102) Molecular dynamics and mutational analysis of the catalytic and translocation cycle of RNA polymerase. BMC Biophysics 5(1):11. Available at: https://www.researchgate.net/publication/225281979_Molecular_dynamics_and_mutational_analysis_of_the_catalytic_and_translocation_cycle_of_RNA_polymerase/figures?lo=1
- Washburn, R.S., and Gottesman, M.E. (2015) Regulation of transcription elongation and termination. Biomolecules 5(2):1063-1078. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496710/pdf/biomolecules-05-01063.pdf
- Zenkin, N., and Yuzenkova, Y. (2015) New insights into the functions of transcription factors that bind the RNA polymerase secondary channel. Biomolecules 5(3):1195-1209. Available at: https://www.mdpi.com/2218-273X/5/3/1195/htm
- Gocheva, V., LeGall, A., Boudvillain, M., Margeat, E., and Nollmann, M. (2015) Direct observation of the translocation mechanism of transcription termination factor Rho. Nuc Acids Res 43(1):10.1093. Available at: https://www.researchgate.net/publication/272162172_Direct_observation_of_the_translocation_mechanism_of_transcription_termination_factor_Rho
- Miki, T.S., Carl, S.H., and Groβhans, H. (2017) Two disctinct transcription termination modes dictated by promoters. Genes & Dev 31:1-10. Available at: https://www.researchgate.net/publication/320350041_Two_distinct_transcription_termination_modes_dictated_by_promoters
- Gurumurthy, A., Shen, Y., Gunn, E.M., Bungert, J. (2018) Phase separation and transcription regulation: Are Super-Enhancers and Locus Control Regions primary sites of transcription complex assembly? BioEssays 1800164. Available at: https://www.researchgate.net/publication/329331157_Phase_Separation_and_Transcription_Regulation_Are_Super-Enhancers_and_Locus_Control_Regions_Primary_Sites_of_Transcription_Complex_Assembly
- Suñé-Pou, M., Prieto-Sánchez, Boyero-Corral, S., Moreno-Castro, C., El Yousfi, Y., Suñé-Negre, J.M., Hernández-Munain, C., and Suñé, C. (2017) Targeting splicing in the treatment of human disease. Genes 8(3):87. Available at: https://www.mdpi.com/2073-4425/8/3/87/htm
- Schaughency, P., Merran, J., and Corden J.L. (2014) Genome-wide mapping of yeast RNA polymerase II termination. PLOS Genetics 10(10):e1004632 Available at: https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1004632
- Nourse, J., Spada, S., and Danckwardt, S. (2020) Emerging roles of RNA 3'-end cleavage and polyadenylation in pathogenesis, diagnosis, and therapy of human disorders. Biomolecules 10(6):915. Available at: https://www.mdpi.com/2218-273X/10/6/915/htm
- Wikipedia contributors. (2020, July 30). Five-prime cap. In Wikipedia, The Free Encyclopedia. Retrieved 05:53, August 11, 2020, from https://en.Wikipedia.org/w/index.php?title=Five-prime_cap&oldid=970240533
- Cortes, T. and Cox, R.A. (2015) Transcription and translation of the rpsJ, rplN and rRNA operons of the tubercle bacillus. Microbiology (2015) 161:719-728. Available at: https://www.microbiologyresearch.org/docserver/fulltext/micro/161/4/719_mic000037.pdf?expires=1597159574&id=id&accname=guest&checksum=6FFC9C066EF41C7799FAE843CE94C49F
- Hein, P.P. and Landick, R. (2010) The bridge helix coordinates movements of modules in RNA polymerase. BMC Biology 8:141. Available at: https://bmcbiol.biomedcentral.com/articles/10.1186/1741-7007-8-141
- Gonatopoulos-Pournatzis, T., and Cowling, V.H. (2014) Cap-binding complex (CBC). Biochem. J. 457:231-242. Available at: https://www.researchgate.net/publication/259392894_Cap-binding_complex_CBC

domain: biology


text:
How To Essentially Lose Weight During Christmas
We must now ask the question, what is really a normal program? Is it one full of junk food and simple carbohydrates that are unhealthy on the whole? The issue must be debated more as to your efficacy of binging on foods which we know are not going that can us reach our longterm goals of health and fitness. The cycle where then the diet works guarantees that the carbohydrate ratio will be met. Naturally why adopting to eat this way may be optimum for most people.
Yes, with a bit uneasy start. But shortly the will adjust, and within 4 days your system will begin changing for your better.Typical foods on a keto guidelines include nuts, whey protein, eggs, bacon, sausage, olive oil, butter, salmon, etc; may contains great protein and fats absolutely no carbs. A vitamin pill is often taken in a Advanced Keto Weight Loss guidelines since it is eat much vegetables. (however you can eat you ought to bowl of salad). It requires strong willpower to remain on keto since if you cheat once or eat something bad muscles will be out of ketosis. A procedure that took 3-7 days now really need to be re-done.
No really need to worry on the foods often be at work party if you bring a dish to share. By bringing your individual food talked about how much there tend to be at least one healthy dish in which you to select from. Fruits and veggies are for you to transport, need no refrigeration and don't spoil promptly. That makes bringing a fresh fruit and veggie plate to share and excellent choice. Or how of the big green salad along with fresh organic fruits, veggies and insane? If you are trying to find a recipe for a yummy healthy lite salad dressing make this happen one: cup extra virgin cold pressed olive oil, cup organic apple cider vinegar, cup fresh squeezed lemon, 1 teaspoon of lemon zest, salt and https://www.dalmatiabooking.com/author/ibfjannette/ pepper to taste. Pour the [answers.com/topic/salad%20dressing salad dressing] in the salad just before serving. Put together.
I are usually following a cyclical ketogenic diet for a married couple of weeks now, along with the results also been amazing already. Not only has myself composition changed (fat loss and no muscle loss), but my performance on my exercise program has improved considerably. I feel more energy throughout the day, more mentally alert - and no hunger pangs associated with most nutrition itineraries. I believe I am very sensitive to insulin changes, and thus the ketogenic diet works well for everybody.
In this regard, it's not logical to stop the diet with a mindset that they is not very effective. The reason because a few obvious methods many individuals who have read and studied the diet and gotten the best weight loss results. Therefore, it is protected to say that the hcg diet program plan works effectively. In fact, hcg diet plan plan may be the fastest connected with losing the pounds. From the statistics of the diet plan, it is that it comprises of low calorie ketosis diet plan menu for women along with many daily injections of the hormone (hcg). You can hcg will be found in primary nutritional supplement stores. [imageshack.us/photos/Strategy%20plan Strategy plan] can be had in many forms. There is liquid hcg diet which works the same way delivering liquids results.
Any time cold left spots, however, it important to label the containers very carefully, using freezer tape using a permanent sign. Try to prevent the older meals near suggestions to avoid having to throw away terminated gifts.
Remember, a person don't are exercising or are active, search for have to account in this in this. You require to provide yourself that isn't proper nutrition to support your activities.
I would recommend keeping your carb intake to under 100 grams on a daily. And Cycle the consumption of the carbs around the busy times of your day as i.e. your workout! And combine your carbs with protein to slow the discharge of the sugars into the blood. At other times, i.e. dinner, or http://advancedketoweightloss.org/ not around training session - eat higher protein and fat meals. Think meats, olive oils, nuts, seeds, eggs, and fibrous green veggies. If you eat this way, you will miss out on 90% of the local supermarkets stock when you've got go searching.

domain: health


text:
Viking 55 Convertible
The Viking Yacht Company‘s 55 Convertible, first introduced in 1996, is about to break new ground. The company recently announced the launch of a new generation of the 55 Convertible.
“Viking first introduced a 55 Convertible to rave reviews for its styling, performance and tournament pedigree,” notes the company’s press release. “Viking would build over 153 of these ground breaking convertibles, which became the most popular boat in its class of that decade. As this new generation 55 Convertible featuring an exclusive high-speed running surface is about to launch, it appears this introduction is in fine company.”
The press release notes that the flying bridge will have a center console command station featuring power-assisted hydraulic steering, single-lever electronic controls, and electronic trolling valves. A freezer, stowage compartments, tackle space, a step box cooler, and engine room access will be built into the mezzanine deck.
The William Bales and Company interior will be a “most inviting interior ideal for relaxing, entertaining or campaigning on the tournament trail for weeks at a time,” notes the release.
Hull number one will feature a pair of MAN V12 1550 CRM engines rated at 1,550 mhp each, and several engine packages will be available from MAN and Caterpillar.


Since it’s finally getting colder, like myself, I’m sure that you all are probably trying to keep warm. Heating your whole house is expensive, so wouldn’t it be nice if there was a way to keep warm and keep your bills more reasonable? Energy conscious engineers in Madrid may be able to help you out!
Many people have been using solar panels to heat the water in their bathroom for several years now, but the engineers at the Madrid University Carlos III and Politécnica say that they can also be used to heat in the winter and cool in the summer large inhabited spaces. They’ve incorporated solar panels into a gas-based system within a larger absorption machine. This would reduce both energy expenditure and CO2 emissions!
The gas engine within this system generates electricity and can in use the residual heat that is produced in the conversion process during winter months. Then, in summer when we are sweltering, the residual heat powers an absorption machine which cools the water to provide air conditioning.
Pedro A. Rodriguez, the primary author of the study, explained that although it is only compulsory for establishments in order to meet the demand for hot water, a very limited number of them escape this by not having either showers or kitchens. In order to make establishments utilize this new energy system, the energy needs of buildings, one must consider the temporal trends in a specific area.
Although at the moment only businesses, train stations such as the Atocha Station in Madrid, and shopping malls have been utilizing this new green technology, maybe someday soon we all will have the ability to use solar panels to reduce both our energy bills and our carbon footprint!
OnAir says: How can you stay green as it gets colder? Let us know your ideas in a comment below!


It was a beautiful day for Positivity in the Park on Tuesday, August 6th. The Mora Fire Department had a course set up for kiddos to navigate with different gear and equipment. Then they pullout out the show stopper…the water hoses! It was a perfect day to be a Junior Firefighters, play in the park with friends and get wet.
Special thanks to coalition members, Christine Sand, for coordinating this event and the Mora Fire Department for their time and dedication to our community.


Subject: Grouper Users - Open Discussion List
- From: "Klug, Lawrence" <>
- To: Chris Hyzer <>, Tom Barton <>, "" <>
- Subject: [grouper-users] Plone integration design
- Date: Thu, 23 Aug 2012 18:11:40 +0000
- Accept-language: en-US
I’ve gotten a bit more clarification about the Plone integration requirements. We want to manage all Plone Groups and Roles in Grouper and “Push” the data to Plone in near real time. So data will move from
Grouper to Plone only. We figure on some custom development on both sides. So we might develop a Change Log consumer to listen for changes on a Plone stem/group and then call Plone API to populate the changes in Plone. Another possibility is a dedicated
LDAP server – we could provision Plone data using PSP and Plone could read data from the LDAP server. What are your thoughts about these ideas?
I just quickly read this:
It seems like the “context” in plone, will be resources in Grouper permissions. When someone creates objects in plone, can there be a trigger to create the resource in Grouper in the right place and assign the permission implications (parents
imply children). Note, this is now possible to do in Grouper WS 2.1.
The “permissions” in plone (View, Modify) could be “actions” in Grouper on the permission definition for the resources above. These seem like they would be configured one time and not change much.
The roles and groups in plone are roles and groups in Grouper.
Finally, you need to decide when you will retrieve the user’s permissions. I would suggest either on login or provision all the permissions to plone so plone doesn’t think they are externalized. Similar to this:
If you do it on login, it could be from Grouper WS, LDAP (concatenate the action and resource into an attribute), or SAML assertion (might still need to concatenate the action/resource).
My gut feel is that to avoid performance problems and maximize uptime to just provision everything to plone and keep it in sync real time (XMPP? Stomp? Other messaging?).
Let us know what you decide to do or if you have more questions
Yes, we are trying to determine how UCLA Service Providers could best leverage Grouper. Will eduMember attributes be sufficiently fine-grained for our applications? If not, how do we approach implementing Grouper
Roles and Permissions? Our pilot project will most likely be the Content Management System. We are using a Zope-based product called “Plone.” What are the steps for moving Plone Roles and Permissions management into Grouper? LDAP delivery via Shibboleth
is an elegant solution, but Roles and Permissions may require another strategy. Thinking this through now could prevent future pain.
We are defining our long-term access management strategy with Grouper. We have tested “eduMember” for transmitting membership info through Shibboleth. Roles and Permissions are internal Grouper attributes
that would not live in the Enterprise Directory(?) Trying to focus on exactly how Roles and Permissions attributes can be consumed by a University Web application now and in the future.
We have two examples where we sync all the permissions to the application since it does DB joins on the assignments, or we dont want grouper as a performance bottleneck or
a runtime dependency. The change log consumer and grouper client handle real time updates (tells it to do a full resync)
 on behalf of Klug, Lawrence
We created a simple demo app to consume isMemberOf attribute via Shibboleth and make a few simple Web Service calls. It works fine. What if we want to use Role and Permission attributes? How would they be transmitted to the Client application?
Could they be released as Shibboleth attributes or direct Web Service call? What are other universities doing?
- [grouper-users] Plone integration design, Klug, Lawrence, 08/23/2012
- [grouper-users] RE: Plone integration design, Chris Hyzer, 08/24/2012
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Those design parameters are my own, from many hours of experimentation. I wasn't trying to create a scale heli. The blade overlap is only by an inch or two and since the rear rotor is elevated by 2" - 3" and canted rearward, blade strikes are not an issue. It may sound strange but this configuration has much better movement authority and manages yaw better. I forgot to mention the rotors turn in opposite directions.
H design quads are not that uncommon. There are a number of them designed after airborne carriers copied from movies, as well as steam punk inspired designs. I was seriously thinking of designing my own steam punk quad. I have many years of model ship building experience. Here's one that's currently being worked on that also shows the carrier and other design possibilities: http://www.rcgroups.com/forums/showthread.php?t=1682868
. I think they are stressing the ship aspect too much, but otherwise it looks pretty cool. Why would you have lifeboats for an airship?


text:
The problem is that instead of the desired icon, there is a combination of icon&Text (img1), and I need only an icon to appear... If there was an option to delete the text I would do that, But I haven't seen such an option - The menu-link text seems to be mandatory (img2).
I though of the css command
text-indent: 200px to remove the text but the font itself is also text... Any ideas how to safely remove the text without removing the icon? Thx,


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Nationality: South African
Hobbies: Scuba Dive, Surf & making music
Fastest Marathon time and when: Shees can’t remember when last I have run a marathon
Years running: Since 2011 (my first KAEM)
Most interesting races: KAEM – I have done a few runs/races, but nothing comes close to the KAEM. It is interesting on all levels. Interesting environment. Interesting conditions. Interesting people…and most Interesting and amazing crew 🙂
Charity: LifeXchange – Mentoring Vulnerable and High Risk Youth
Why KAEM: I stumbled upon her many years ago and totally fell head over heals in love with her. She is tough and not gentile at all, yet she is consistent. Even though she is not very sexy, she is super hot! She is not the friendliest, yet radiates an indescribable beautify. But most of all, she allows me to be and become, the best version of myself.
KAEMs completed: 3

domain: hobby


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- 55.00 - 90.00 INR
Our valuable clients can avail from us a delectable range of organic Basmati Rice that is processed under strict hygienic conditions. This rice is hugely consumed by our clients across the nation, owing to its delicious taste, high nutritional value, regular size and natural flavor. The offered rice is grown in a suitable environment and is passed through different milling processes. We ensure our prestigious clients that our indian Basmati Rice is hygienically processed and packed, which ensures its safe to consume features & high quality.
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domain: food


text:
The COVID-19 pandemic broke out in China in December 2019, and by March 2020 has affected almost all countries around the globe causing national governments to introduce a range of unprecedented measures. As for now, nearly half of the world’s population is on lockdown endorsed to control the spread of the virus (World Health Organization, “COVID-19 Situation Report – 72” 2). The purpose of this paper is to discuss the isolation measures, their efficiency in controlling the pandemic, and their psychological effect.
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Pandemic Control Measures
The first country to introduce quarantine measures was China, where the virus first broke out in December 2019. The province of Hubei, where the virus originated, was put on a two-month lockdown isolating it from the rest of China, and a range of strict measures was imposed all over the country. They included the cancellation of all sports and cultural events, a ban on private transport, closure of non-essential businesses, remote working measures, and control of public movement. The drastic measures proved to be successful, as the number of COVID-19 cases has been gradually reducing since the introduction of the quarantine, and the country is slowly returning back to normal (World Health Organization, “China’s COVID-19 Response”). China’s success in managing the pandemic encouraged other countries to adopt the same approach and introduce a similar range of measures.
The quarantine measures are mainly aimed at the prevention of the person-to-person transition of the virus. As a first response, many governments and air carriers canceled and reduced the number of flights to and from the affected regions and imposed travel restrictions of varying intensity, up to a total border closure. However, as the virus had already been spreading among the local communities, more severe measures needed to be introduced to control the pandemic. The list of these measures put together by the World Health Organization includes “flexible work arrangements, distant learning, reducing and avoiding crowding, closure of non-essential facilities and services, staying-at-home measures and movement restrictions” (World Health Organization, “COVID-19 Situation Report – 72” 2). Many businesses had to switch to remote work, increase the usage of online communication platforms, revise work schedules, introduce social distancing measures on worksites and reduce the number of employees. Many retail outlets were forced to close down or turn to home delivery. The quarantine has dramatically changed the daily life of millions of people all over the world.
The Effects of Pandemic Control Measures
With the pandemic control measures constantly changing all aspects of everyday life, their effects are yet to be fully researched and examined. While the measures are generally believed to be reasonable and efficient, their economic and social impact is hard to predict. With the communities’ primary focus being on the COVID-19 control and prevention, other aspects of people’s lives have moved to the background having to adapt to the changing environment and face a number of inconveniences.
Social isolation provides many challenges that people all over the world have to face. The first is employment problems, as many people have lost their jobs, are unable to work due to the quarantine, or have been forced to work in an unfamiliar environment. As Zaharieva states, “keeping a job and earning a living has become uncertain, especially for those who are already in a precarious situation, leading to greater levels of stress and anxiety” (Zaharieva). The stress of not being able to resolve business issues, the uncertainty of the market situation, communication difficulties, and the challenges of self-organization all add up to the pressure and create an increased level of stress for those who work at home.
The second problem is the feeling of loneliness and isolation that people under the quarantine have to face. It is especially dangerous for those who live alone, have no close friends or relatives, and have troubles communicating over the internet. The reduction of social contacts is mostly felt by the elderly, and the more isolated a person is, the more stress and anxiety they experience (Adam). Combined with the difficulties of contacting a doctor or psychotherapist, the increased sense of loneliness can lead to a number of mental health problems, depression, and the aggravation of chronic illnesses. Even for young and healthy people, being deprived of external social contacts for weeks can be a challenge.
These conclusions can be illustrated with my personal experience. I am a security guard working at a plant, and I am one of the people who are required to work during the quarantine. Most of my immediate family also continues to work. I think that it is very good in terms of my mental health, as I do not have to experience the effects of isolation. Having to communicate with my colleagues, I do not feel alone and stressed out by the quarantine.
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I think that people who stay at home have to live through a great deal of stress and anxiety being deprived of their habitual way of life and daily routine. Being forced to stay at home, follow the news, and communicate with their friends and colleagues only by means of online tools, they have little else to discuss as the spread of the virus and the rumors connected with it. Although neither I nor my relatives have been abroad or made contact with people who might be affected by the virus and we feel no immediate danger, the pandemic of social media panic is still affecting us to some point. Nevertheless, I understand that all these measures are necessary and the most important thing now is to stay healthy and prevent any exposure to the virus.
With the COVID-19 control and prevention being the primary international task, it is also necessary for national governments to focus on the problems that people living under the quarantine have to face. As Zaharieva notes, “the impact of isolation and loneliness should not be underestimated or fall to the bottom of politicians’ lists of priorities as inaction now will lead to high human and financial costs later on” (Zaharieva). Several measures can be introduced, such as the reform of the healthcare system, the organization of psychological support lines and volunteer campaigns, and the development of online social tools. The possibility to consult a health professional or get psychological help can be of great benefit to people experiencing increased stress and anxiety. It can also help to reduce the long-term effects of the pandemic and contribute to the recovery of national communities after the quarantine.
In the reality transformed by the pandemic and the quarantine measures aimed to prevent the spread of the virus, people all over the world have to face a number of challenges. Isolated working conditions combined with the lack of social contacts cause people to experience an increased feeling of stress and anxiety, and even those who continue to work face some difficulties in organizing their daily routine. It is considered one of the government’s priorities to address the problems faced by the people under quarantine and introduce a number of measures to help them live through it with the smallest possible consequences.
Adam, David. “The Effects of Physical Isolation on the Pandemic Quantified”. The Scientist, 2020. Web.
Zaharieva Radost. “The Dangers of Social Isolation During a Pandemic”. ESPA, 2020. Web.
World Health Organization. “Coronavirus Disease 2019 (COVID-19). Situation Report – 72”. World Health Organization, 2020. Web.
World Health Organization. “China Shows COVID-19 Responses Must Be Tailored to the Local Context”. World Health Organization, 2020. Web.

domain: medical


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The Guji Region is one of the most remote coffee regions, but also produces some of the most legendary coffees. The Alaka mill processed this coffee grown by the farmers in the surrounding village. It is a natural processed coffee to accentuate the fruity and sweet tastes of this Ethiopian bean.
The heirloom varietals grown here are wildly fruity, and you want to roast this bean light in order to enjoy them. You want to roast this like you would any other natural processed Ethiopia coffee. Just out of first cracks, you get sweet cherry and raspberry notes, some floral aroma (jasmine), and some acidity. It's a grade 1 process, and the beans are very clean and well sorted. I prefer brewing this as a pourover versus a french press. The french press does have a slight murkiness, whereas a pourover is clean and crisp.
August 2018 arrival in USA.


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Learn 2-0 in USBC Masters
GREEN BAY, Wis. -- Erie's Bob Learn Jr. won twice in match play Friday to advance to today's play in the USBC Senior Masters Bowling Championship at the Ashwaubenon Bowling Alley.
Learn, 53, who qualified for the top 64 in 59th place after 15 qualifying games this week, defeated Sam Ventura 611-591 and Tommy Baker 699-694 to move to the round of 16 in the winners' bracket in the double-elimination tournament.
Learn will face Duane Mellinger today at 8:30 a.m.
First prize in the $100,000 major tournament is $16,000.


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domain: entertainment


New Orleans Saints NFL Burst Premium Twill Jacket.
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Immigrants who arrived after 1999 were 45% less likely to receive state benefits or tax credits than UK natives in the period 2000-2011
Well quite. But that's immigration over the last 14 years. What about all the immigrants that arrived in the wake of the Second World War? You know, that little period of 54 years prior to 1999?
Yes, a large amount of them are hard workers and undoubtedly do contribute; but where I live, there's an awful lot who don't work and are quite happy to sit on their arses claiming all the benefits that they can, whilst at the same time running criminal rackets such as drug dealing, extortion, fraud, prostitution and child exploitation.
And another thing (and I know that they aren't your words), what exactly constitutes a "UK Native?" please?
but the only immigrants I know work their arses off.
I suspect then that the only immigrants you know are from Eastern Europe - that being the case, I would concur.
I also suspect that these reports encouraging immigration and multi-culturalism are largely written by immigrants themselves and also persons who have little or no exposure to towns and cities where the immigrant population (largely from the Indian subcontinent) has now exceeded the existing North-Western European, caucasian population. These towns have rapidly declining standards of living, soaring crime rates, "no-go" areas for white people and in some cases areas where the Police fear to tread unless they go in mob-handed.
I'm not totally anti-immigration, but I am for selective immigration. The population of the UK currently stands at about 63 million - it's bursting at the seams, there are millions out of work, the NHS and other services are on their uppers yet still we are happy to let in criminals and asylum seekers who have no right to be here and then spend an aeon sending them back or putting them in OUR prisons.
At the current rate, estimates are that the population of the UK will be 80 million by 2020 - how is that going to help the country to function better?

domain: sociology


Apple Car electric car characteristics
|Among the many promising developments of Apple, a special place, and, consequently, increased interest, is occupied by the project to create an electric car Apple Car. Ming-Chi Kuo, an insider analyst specializing in Apple products, famous for his sales forecasts, provided some information about the future car of the high-tech giant. |
First of all, the analyst confirmed the report that the production of the car from Apple will be carried out in partnership with the largest Korean automaker Hyundai. At the same time, Apple Car production will be deployed in the United States, based on the factories of the second largest Korean automaker Kia. The Hyundai Mobis division will take part in the development of individual components of the car, and the electric car itself will be created on the basis of the E-GMP platform, presented by Hyundai in 2020.
The Hyundai platform will be able to guarantee impressive technical characteristics for the Apple Car. So the speed of acceleration to 100 km / h will be only 3.5 seconds, and the autonomous radius of the trip will be more than 500 km at a maximum speed of 260 km / h. The battery pack of the E-GMP platform supports fast charging technology, with which 80% of the energy can be replenished in just 18 minutes.
According to Ming-Chi Kuo, the development of the Apple Car will take a long time. The analyst notes that even to create the next iPhone, " it takes about 18-24 months from the initial specification to mass production." Given that Apple has no experience in the automotive industry, the greater complexity of the car compared to a smartphone (the car has 40-50 times more components than a smartphone) and the complexity of the supply chain, the Apple Car should not be expected to enter the market until 2025.
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Channel Orange was released in July 2012, which feels like eons ago in the digital age. In a time where we know about a celeb’s every move thanks to Instagram, Twitter and paparazzi, Frank Ocean is barely a blip on the radar. He dropped his debut on us and virtually left us in the dark. Fans and critics are itching for a follow-up.
Nabil Elderkin, Ocean’s trusty visual accomplice, gave us hope that album numero dos is in the foreseeable future. He shared an Instagram photo of our elusive singer hunched over in a studio, with the caption: “Frank in the lab.. Making sophomore album game.” Before his 2013 You’re Not Dead… tour got cut short due to a torn vocal chord, he released three new songs, but there’s no telling if they were album cuts or just loosies to remind us that, well, he’s still breathing.
Creatively, many are anxious to see where Frank’s head is after such a lengthy sabbatical. He’s got a lot of ground to cover. Aside from filling us in on the massive time gap (how were your travels to Shanghai, Frank?), these are 5 things we’d like to see on the new album that will assure it was well worth the wait. –Stacy-Ann Ellis
All Photo Credits: Getty Images


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Denver is probably one of the best hidden secrets in the U.S. This is not just true because winters there feel incredibly mild, there is constant sunshine and people are just way too friendly—but also because there is more vegan food than your heart desires.
Start your exploration by visiting The Corner Beet—a vibrant breakfast, brunch and lunch place with a variety of vegan açaí bowls, colorful smoothies and delicious toasts. All toasts come on freshly baked bread. The Ban Mi is our favorite. Perfectly crispy tofu, avocado and jalapeños on top of a thin layer of vegan mayo. Perfection.
If you are looking for a casual dinner and real comfort food, City O’ City is your spot. Most dishes are vegetarian or can be made vegetarian. The warm Brussels sprouts salad is a must and the waffles and chicken (crusted cauliflower deliciousness!) is an absolutely winner—no matter if you come for brunch or dinner. I also loved the kimchi pizza—the dough was fluffy and melted in your mouth.
If you are looking for a quick, yet utterly satisfying bite, check out Quiero Arepas for, well yes, arepas. The vegan arepa comes with grilled plantains, avocado and black beans. It’s hands down one of the best meals I have ever had. I recommend an extra side of plantains.
For a tiny bit fancier feel, Watercourse Foods is an absolute must. The cauliflower wings are delicious—my favorite was the siracha apricot flavor. I also loved the sweet potato gnocci that came with a pumpkin puree. Another must is the 3 taco platter. All dishes are super indulgent and highly sharable.
If you are in the mood for a drink—check out Stem Ciders. They offer a variety of sweet and dry local ciders, as well as locally sourced kombucha. I highly recommend the lavender flavor by Happy Leaf.
Have we missed any must-taste vegan food in Denver? Let us know!
Also by Isabelle: How To Share The Burden Of Emotional Labor & Improve Your Relationship
Get more like this—Subscribe to our daily inspirational newsletter for exclusive content!
Photo: Watercourse Food; Isabelle Steichen


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Bright City is a family of artists who serve and lead the worship life of St Peter’s Brighton.
With authentic and brave creativity, we want to see our church, our city and our world come alive through worshipping Jesus.
We hold regular Bright City Team Nights open to anyone who resonates with our vision.
The Team Night dates this term are:
Monday 8 May: Gloucester Place (One Church)
Monday 5 June: Venue TBC
Friday 7 July: Album Launch
We run creative streams for all artists who share our vision – such as musicians, songwriters, producers, photographers and dancers.
Contact us to find out more.
Bright City record
The self-titled debut album from Bright City presents 13 tracks of faith and hope.
From the anthemic title track ‘Bright City’ via the explosive first single ‘Colour,’ through to more tender moments including 'I Will Rest' and 'Broken' - the album aims to point people to their Creator.
Visit www.brightcityuk.com for more information.


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Sigma-Aldrich Corporation and Sangamo BioSciences, Inc. announced a significant alliance focused on the development of high value laboratory research reagents based upon Sangamo's ZFP technology. The alliance combines Sigma's global marketing capabilities and reputation as a provider of high quality products and kits for scientific research with Sangamo's expertise in the field of ZFPs.
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NRS is not 'makin' progress' on the Raccoon River
on Thursday, April 22, 2021
Water and Land News
April 22, 2021 - Des Moines, IA - Last week, Iowa’s Raccoon River landed at #9 on America’s Most Endangered Rivers list for 2021. Iowa Secretary of Agriculture Mike Naig dismissed the alarm of many Iowans, calling the list “propaganda” and saying the report was ‘not rooted in fact.’ Secretary Naig said the “so-called” report was just a fundraising tool for environmental non-profits.
IEC challenges Secretary Naig’s assertion, asking the question: propaganda for whom?
Who really benefits from such "propaganda?" Aquatic life in the river and downstream? Those who fish in the river to supplement their diet? Gardeners who use the water to sustain their vegetable patch? Those who paddle or swim in its waters? The hundreds of thousands of Iowans who rely on the river’s water to cook their meals, mix their infant’s bottle, or fill their glasses at the dinner table?
The Raccoon River’s three tributary branches snake through 10 counties before combining and joining with the Des Moines River, itself a tributary to the Mississippi. Land in those counties houses hundreds of concentrated animal feeding operations (CAFOs) and millions of acres of crop production, the primary sources of nitrate, bacteria, and other pollution. The river is also a main source of drinking water for many communities in the watershed. Of the 500,000 consumers in central Iowa, dozens are rural communities that rely on the river for drinking water, including Xenia Rural Water and Warren Rural Water.
“I grew up in Carroll, along the banks of the Middle Raccoon. This is a river, and a population of Iowans, under siege. The Nutrient Reduction Strategy, which Secretary Naig and other ag leaders claim will ‘solve’ the problems of this river and the many others in Iowa, has no actual nitrogen pollution reductions to show to date. It is the “progress” of the NRS that is not based in fact,” says Ingrid Gronstal, Water Program Director with the Iowa Environmental Council.
IDALS and other leaders continuously point to a relatively small number of conservation practices implemented over eight years as progress, while the actual amount of nitrate in the water – one of the main goals of this project – has doubled since 2000.
Iowans and IEC understand that the initiatives in the state’s Nutrient Reduction Strategy, adopted in 2013, take time to effect change, but after eight years this voluntary approach has yielded no measurable improvement in the water quality of the Raccoon River. Financial incentives don’t appear to be motivators for significant change, as $2 million for conservation project funding in the North Raccoon River Watershed remained unclaimed 2020, despite a 90% cost-share offer. That cost-share means landowners would only need to pay 10% of the costs to implement conservation practices.
Here are facts, based on measurable data, to support the inclusion of the Raccoon River listing:
“We were not at all surprised to see the Raccoon River included on this list,” says Alicia Vasto with IEC. “Data make it clear that those living and making their livelihoods in that watershed are right to be concerned about the health of this important waterway and call for more action from Secretary Naig and other leaders to protect public health and recreation for Iowans.”
IEC is advocating for improved state policy to drive land use change and curb nutrients through mandatory practices for non-point sources of pollution and implementing basic standards of care. If state policymakers will not do it, it is time for federal agencies to step in.
“We need benchmarks, timelines, and a proper balance between private and public interests,” says Gronstal. “One-sixth of all Iowans get their drinking water from the Raccoon River. Iowans in communities and farms spanning hundreds of miles live, work, and play along this river. If the state is serious about addressing water quality, our leaders need to stop saying we’re heading in the right direction and start to deliver real pollution reduction results.”
- clean water
- drinking water
- nitrate pollution
- nutrient reduction strategy
- public health
- sustainable agriculture
- water quality