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Braun Melsungen AG, Melsungen, Germany) was used. Planning of Genetically Engineered Na and FGF2-SCs?ve SCs for Transplantation Within Composite Chitosan Nerve Grafts Three times before medical procedure neonatal rat SCs were modified genetically via non-viral transfection as described earlier (see section non-viral Genetic Anatomist of SCs). Rats by Nina Dietzmeyer, Zhong Huang, Tobias Schning, Shimon Rochkind, Mara Almog, Zvi Nevo, Thorsten Lieke, Svenja Kankowski and Kirsten Haastert-Talini in Cell Transplantation Abstract In today’s study we looked into the suitability of the book hyaluronic acidClaminin hydrogel (HAL) as luminal filler and carrier program for co-transplanted cells within a amalgamated chitosan-based nerve Escitalopram graft (CNG) within a rat important nerve defect model. The HAL was designed to improve the efficiency of our artificial nerve manuals by giving extra structural and molecular support to regrowing axons. We stuffed hollow CNGs or two-chambered nerve manuals with an placed longitudinal chitosan film (CNG[F]s), with cell-free HAL or cell-free HA or suspended either na additionally?ve Schwann cells (SCs) or fibroblast growth aspect 2-overexpressing Schwann cells (FGF2-SCs) inside the gels. We subjected feminine Lewis rats to instant 15 mm sciatic nerve distance reconstruction and comprehensively likened axonal and useful regeneration parameters using the yellow metal regular autologous nerve graft (ANG) fix. Electric motor recovery was surveyed through electrodiagnostic measurements at 60, 90, and 120 times post-reconstruction. Upon explantation after 120 times, lower limb focus on muscles had been harvested for computation of muscle-weight ratios. Semi-thin cross-sections of nerve sections distal towards the grafts had been examined histomorphometrically. After 120 times of recovery, just ANG treatment resulted in full electric motor recovery. Amazingly, regeneration outcomes uncovered no regeneration-supportive aftereffect of HAL by itself as well as an impairment of peripheral nerve regeneration when coupled with SCs and FGF2-SCs. Furthermore, complementary in vitro research, executed to elucidate the nice reason behind this unforeseen harmful result, Escitalopram uncovered that SCs and FGF2-SCs suspended inside the hydrogel downregulated gene expression of regeneration-supporting neurotrophic points Escitalopram relatively. To conclude, cell-free HAL in its current formulation didn’t be eligible for optimizing regeneration result through CNG[F]s. Furthermore, we demonstrate our HAL, when utilized being a carrier program Rabbit Polyclonal to 14-3-3 zeta (phospho-Ser58) for co-transplanted SCs, transformed their gene appearance profile and deteriorated the pro-regenerative milieu inside the nerve manuals. = 3 indie qRT-PCR works per cell lifestyle and type condition. For na?ve SC cultured in HAL, however, the reduced proliferation aswell as low cell density upon cell harvest (Body 5) resulted in some cDNA, that was just enough for pooling cDNA for = 2 indie analyses. Open up in another home window Fig 5. Representative images of phase-contrast microscopy of Schwann cells (SC) seeded in either SC-specific lifestyle moderate (K+, A), hyaluronic acidity (HA, B), and hyaluronic acidClaminin hydrogel (HAL, C). Three times after seeding, cells cultured in K+ (A) and HA (B) demonstrated an average bipolar morphology. Proliferation from the seeded 350,000 cells resulted in a thick cell layer in the well surface. In HAL condition (C), SC, nevertheless, uncovered a different morphology and higher apoptosis price (cell detritus is certainly indicated by arrows). Size bar shows 100 m. In Vitro Evaluation of Immunocompatibility Between Receiver Spleen (Spl) and Lymph Node (LN) Cells and Donor SCs via [3 H]thymidine Incorporation Assay Since enough amounts of neonatal rat SCs because of this study weren’t accessible from Lewis LEW/OrlRj breeds in realistic time (little liter sizes and low proliferation price of major cells), we made a decision to make use of neonatal SCs from Wistar RjHan:WI breeds. The transfer of genetically customized SCs produced from Wistar RjHan:WI rats within CNGs in to the receiver LEW/OrlRj rats shows, nevertheless, an allogenic transplantation, which comprises Escitalopram Escitalopram the chance.
Supplementary MaterialsSupplementary Table 1 Sequences of Primers Used in the Paper mmc1. rapamycin and cisplatin led to a higher cytotoxic activity of NK cells, smaller UEC volume and longer survival rate and and further clarifies the mechanism of rapamycin on immune regulation of the UEC microenvironment. Materials and Methods Antibodies Anti-human IL-27 antibodies (Abs) and anti-human PCNA were purchased from Abcam (USA); Phycoerythrin (PE)-conjugated anti-human IL-27, PE-conjugated anti-human WSX-1, Allophycocyanin (APC)-conjugated anti-human gp130, Brilliant Violet 421 (BV421)Cconjugated anti-human CD56, phycoerythrin-cyanine 7 (PE-Cy7)Cconjugated anti-human CD16, fluorescein (FITC)-conjugated anti-human NKG2D, phycoerythrin-cyanine 7 (PE-Cy7)Cconjugated anti-human NKp44, APC-conjugated anti-human NKp46, PE-conjugated anti-human NKp30, FITC-conjugated anti-human KIR2DL1, PE-conjugated anti-human KIR3DL1, APC-conjugated anti-human IFN-, PE-conjugated anti-human perforin, Brilliant Violet 421 (BV421)Cconjugated anti-human Granzyme B, PE-conjugated anti-human Fas, APC-conjugated anti-human FasL, PE/Cy5.5-conjugated anti-human Ki-67, and PE-conjugated anti-human Bcl-xL were purchased from BD Biosciences (San Jose, CA). Patients and Sample Collection The protocol for this study was approved by the Human Research Ethics Committee of Obstetrics and Gynecology Hospital, Fudan University, and written informed consent was obtained from all participants. All the normal endometrial tissues, highly differentiated UEC, moderately differentiated UEC and poorly differentiated UEC tissues were obtained by laparoscopy from 45 patients (mean age 47.8 years; range 36C54 years) at the Obstetrics and Gynecology Hospital of Fudan University. All of the samples were confirmed histologically. Ten UEC patients had a highly differentiated degree of UEC, 10 UEC patients had a moderately differentiated degree of UEC, and 10 UEC patients had a poorly differentiated degree of the disease. Normal endometrium in the secretory phase of the cycle was obtained through hysterectomy from patients with leiomyoma (15 cases) as normal control samples. No patients took any medications or received hormonal therapy within 6 months prior to surgery. Furthermore, Hexachlorophene the peripheral bloodstream was gathered from 72 healthful fertile ladies (mean age group 28.7 years; range 23-37 years). Cells Lines The human being endometrial carcinoma cell lines (Ishikawa, RL95-2, and KLE cells) had been from the cell standard bank of Rabbit Polyclonal to ACOT1 Chinese language Academy of Technology (Shanghai, China). Ishikawa was cultivated in RPMI-1640 moderate (Gibco, USA) supplemented with 10% fetal bovine serum (FBS; Hyclone, Logan, UT), 100 U/ml penicillin, and 100 mg/ml streptomycin. RL95-2 and KLE cells had been expanded in Hexachlorophene DMEM/F12 moderate (Gibco, USA) supplemented with 10% fetal bovine serum (FBS; Hyclone, Logan, UT), 100 U/ml penicillin, and 100 mg/ml streptomycin. Immunohistochemistry Paraffin areas (5 M) of regular endometrium and UEC cells from patients had been dehydrated in graded ethanol and incubated with hydrogen peroxide and 1% bovine serum albumin/TBS to stop endogenous peroxidase. The examples were after that incubated with mouse anti-human IL-27 (10 g/ml, Abcam, USA) or mouse IgG isotype over night at 4C inside a humid chamber. After cleaning 3 x with TBS, the areas had been overlaid with peroxidase-conjugated goat anti-mouse IgG, as well as the reaction originated with 3,counterstained and 3-diaminobenzidine with hematoxylin. Purification of NK Cells Peripheral bloodstream mononuclear cells (PBMCs) had been isolated from healthful fertile women. Human being NK cells had been isolated from PBMCs using magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany) for co-culture tests Hexachlorophene and cell transfer. Cell Co-Culture We acquired the IL-27-overexpressed UECC (IL-27+) and control UECC (mock) through transfection with GV230-IL-27 plasmid and GV230-vector plasmid (GenePharma, Shanghai, China), and these UECCs had been treated with or without rapamycin (100 nM, Sigma, USA) and/or cisplatin (10 M, Sigma) for 48 hours. Cell supernatants had been discarded after that, and these cells were washed with phosphate-buffered solution (PBS) and co-cultured with NK cells from peripheral.
Supplementary MaterialsSupplementary materials 1 (AVI 22428 kb) 12195_2016_471_MOESM1_ESM. electrotaxis can be seen in isolated cells at intermediate electrical areas also, recommending an adhesion-independent part of E-cadherin in regulating electrotaxis. In extra support of the adhesion-independent part of E-cadherin, isolated cells with minimal E-cadherin manifestation reoriented in a applied electrical field 60% quicker than control. These outcomes possess implications for the part of E-cadherin manifestation in electrotaxis and demonstrate proof-of-concept of the automated algorithm that’s broadly applicable towards the evaluation of collective migration in an array of physiological and pathophysiological contexts. Electronic supplementary materials The online edition of this content (doi:10.1007/s12195-016-0471-6) contains supplementary materials, which is open to authorized users. viral transduction, which requires microbiological equipment and techniques. Used fluorescent dyes Commonly, while basic in application, reduce fluorescence with age group as cells separate and spread dye between girl cells. Site-specific fluorescent antibodies are costly and often need fixation and permeabilization of cells to be able to imagine internal cell parts. An ideal means to fix monitor cell migration requires the advancement or computerized algorithms with the capacity of control phase-contrast pictures of label-free cells. This algorithm would simplify experimental Rabbit polyclonal to USP37 protocols while providing powerful data processing significantly. Monitoring label-free cells within clusters isn’t straightforward due to the low degree of comparison at cell limitations. There are strategies which raise the comparison between cells. For instance, third-harmonic era (THG) supplies the capability to analyze liquids near lipid membranes12 and continues to Metaproterenol Sulfate be used for monitoring lineage of cells inside the zebrafish blastocyst, where fluorescent staining will be as well challenging.31 Ptychography, which enhances comparison by comparing diffraction patterns to brightfield pictures, offers garnered interest like a label-free imaging technique lately.24 Multi-photon methods such as for example THG and multiple camera techniques tend to be unavailable for the normal biological lab, whereas phase-contrast microscopy is ubiquitous in cells culture facilities. There’s been significant improvement in automating Metaproterenol Sulfate the evaluation of phase-contrast microscopy pictures. The automation of determining isolated, single cells offers proved challenging, but could be accomplished using trained background advantage and subtraction recognition.9 The problem of separating adjacent cells continues to be a concern and isn’t easily overcome without combining fluorescent imaging of intercellular components like the nucleus.45 inside a crowded environment Even, morphological properties of cells could be recognized using Fourier change based feature detection.1 While that is useful, especially in high-throughput medication verification, the spatiotemporal location of cells analyzed in this method is not produced. Using a morphological watershed, cell boundaries can be detected but often require additional, computationally intense post-processing steps.46 Despite these advances, the spatial resolution for segmenting cells in a clustered or crowded environment is still poor; in fact, relying solely on phase-contrast images typically provides only enough resolution to differentiate between regions of one cell type versus another.18 Here, we develop a label-free Metaproterenol Sulfate tracking algorithm capable of identifying individual cells within a migrating cell cluster. A trademark of the method described herein is that images are cropped into multiple, overlapping images in such a way to increase the robustness of image processing techniques. Individual cells are tracked sequentially through frames so that the previous location can be used to infer the location of a region of interest. We apply this algorithm to study the electrotaxis of clustered epithelial cells in a high throughput manner. We and others have previously shown showed that clustered cells exhibit better electrotactic response than isolated counterparts.20,22 We sought to investigate the role of the expression of E-cadherin, a cell surface receptor that mediates cellCcell adhesion,40,44 in the enhanced electrotaxis of clustered cells. E-cadherin expression can be downregulated in tumor development30 frequently, 41 and epithelial-derived tumor cells screen solid electrotaxis in cells tradition typically.22,48,50 The association between metastatic potential and electrotaxis could be due to the endogenous electric field which arises in the interface of cancerous and noncancerous tissue.11,25 Indeed, endogenous electric fields have already been measured inside a clinical Metaproterenol Sulfate establishing and Metaproterenol Sulfate were proven to give a diagnostic modality for detecting breast cancer.11 Moreover, inhibition of E-cadherin with.
Supplementary Materialscells-08-01413-s001. their results on CDK19 and CDK8, kinome profiling determined many off-target kinases for both Cmpd4 and Cmpd3, which could lead to their toxicity. Off-target actions might have been achieved in the scholarly research of Clarke et al. credited to saturated in vivo dosages of Cmpd4 and Cmpd3, chosen for the capability to inhibit STAT1 S727 phosphorylation in tumor xenografts. We display right here that STAT1 S727 phosphorylation can be induced by different cytokines and tension stimuli in CDK8/19-independent manner, indicating that it is not a reliable pharmacodynamic marker of CDK8/19 activity. These results illustrate the need for careful off-target analysis and dose selection in the development of CDK8/19 inhibitors. = 12); Senexin B (4 M, = 12); dCA (1 M, = 12); Cmpd3 (1 M, = 12); Cmpd4 (1 M, = 12); 15w (1 M, = 12); negative control (egg water, = 6); 3,4-dichloroaniline (8 mg/L, = 6) (positive toxicity control). The experiment was performed in triplicate with biological replicates. Fish embryos were examined at 24, Tedalinab 48, 72 and 96 h post compound addition and scored as healthy, abnormal or dead. Statistical significance of difference in percentage of healthy embryos was evaluated by RM two-way ANOVA, followed by Dunnetts multiple comparison test (****: < 0.0001). (B) Evaluation of compound toxicity using 24 hpf non-dechorionated Rabbit Polyclonal to CPN2 zebrafish embryos (TU strain). Data from multiple technical replicate experiments are pooled together to calculate the overall percentage of healthy normal embryos in the presence of different Tedalinab compounds. Risk ratios (RR) of different treatments compared with vehicle-treated group (DMSO) were calculated to compare risks of unhealthy development under exposure to different inhibitors. Asterisk (*) indicates RR > 5 and pound sign (#) Tedalinab indicates RR > 10. See Table S2 for statistical analysis. Th same analysis was conducted on a larger scale using TU strain zebrafish embryos without dechorionation. Figure 2B shows the fractions of healthy larvae 3, 4 and 5 days after the addition of different compounds at different concentrations (no noticeable phenotypes were detected after the first two days). Statistical evaluation of the differences between the control and inhibitor-treated zebrafish is presented in Table S2. This analysis confirmed very strong toxicity of Cmpd4 at all the tested concentrations (0.5 M, 1 M and 2 M), followed by Cmpd3 (2 M) and dCA (2 M). Hence, CDK8/19 inhibitors with reportedly high selectivity showed wide differences in their in vivo toxicity, with Cmpd4 displaying uniquely high toxicity. 3.2. Toxicity of CDK8/19 Inhibitors Does Not Correlate with the Potency or Stability of CDK8 and CDK19 Inhibition in Cell-Based Assays We have used a Tedalinab panel of 293-derived cell lines with CRISPR knockout of CDK8 (8KO), CDK19 (19KO) or both CDK8 and CDK19 (double knockout, dKO) , as well as wild type (WT) cells to measure the effects of CDK8, CDK19 and different CDK8/19 inhibitors on gene expression. Cells were pre-treated with different concentrations of CDK8/19 inhibitors for 1 h and then 10 ng/mL TNF, an inducer of transcription factor NFB, which is potentiated by CDK8/19 , was added for 2 h before RNA extraction. QPCR Tedalinab was carried out to quantify CDK8/19-regulated expression of NFB-inducible genes CXCL1 and CXCL8 and the basal expression of MYC. In the absence of inhibitors, all three genes showed similar expression in the WT, 8KO and 19KO cells treated with TNF but were strongly downregulated in dKO cells (Figure S2), indicating that CDK8 and CDK19 are both efficient in regulating these genes. As shown in Figure 3, none of the five CDK8/19 inhibitors affected the expression of the three genes in dKO cells (IC50 >> 1 M), indicating that their effect on NFB was CDK8/19 specific. All the inhibitors, however, had very similar effects on the WT, 8KO and 19KO cells.
Supplementary Materials Appendix S1: Supporting information JBM-108-581-s001. showed layers of airbrushed fiber mats supported human dermal fibroblast growth and extracellular matrix development throughout the scaffold. When implanted subcutaneously, hierarchical scaffolds facilitated higher cell infiltration and cells development than airbrushed dietary fiber mats. Fibronectin matrix constructed in vitro through the entire hierarchical scaffold survived decellularization and offered a cross substrate for recellularization with mesenchymal stromal cells. These outcomes demonstrate that by merging FDM and airbrushing methods we are able to engineer customizable hierarchical scaffolds for heavy cells that support improved cell development and infiltration. aircraft can be near 10 m. The coating thickness, or z quality, is limited from the nozzle thickness and it is on the purchase of 60 m (Kuznetsov, Solonin, Urzhumtsev, Schilling, & Tavitov, 2018). The perfect pore size range for cells engineering is known as 50C300 m (Karageorgiou & Kaplan, 2005; Oh, Recreation area, Kim, & Lee, 2007). These pore sizes are attainable with FDM theoretically, nevertheless, the porosity in that scaffold will be below 60% as constrained from the diameter from the imprinted struts. Large, open up macroporous structure of 3D printed scaffolds provides minimal surface for cell growth and attachment. Airbrushing may be used to offer additional surface inside the 3D imprinted scaffold macropores for cell development and development while minimally affecting the overall porosity of the devices. The framework of the 3D printed struts separates the airbrushed layers while maintaining the porosity of the fiber mat. Furthermore, cells rely on signaling cues and attachments points from the underlying matrix which are absent in scaffolds printed by FDM but could be provided in airbrushed fiber mats. Overall, the benefits of and challenges faced by FDM make it a perfect pairing for airbrushed fiber mat integration (Chen et al., 2019; De Mori, Pe?a Fernndez, Blunn, Tozzi, & Roldo, 2018; Mellor et al., 2017; Moroni et al., 2008; Rampichov et al., 2018). Based on these considerations, we have developed a method of creating hierarchical scaffolds combining airbrushed microfiber mats periodically interspersed within a FDM 3D printed support as a way to open up the fiber mat structure to allow for improved cell penetration while maintaining the rapid manufacturing and customization and improving the cell interactivity of 3D printed scaffolds. The airbrushed mat is formed out of a biodegradable tyrosine polycarbonate that has been used for bone regeneration in the past and degrades on the time frame of months allowing for the natural ECM to take over (Jinku et al., 2015; Kim et al., 2015; Zhang et al., 2016). Culturing human dermal fibroblasts upon the scaffold prior Pyrithioxin to decellularization allowed for the functionalization of the hierarchical scaffold with cell\derived ECM to create a hybrid natural and synthetic structure. These hierarchical scaffolds show cell penetration, extensive fibronectin deposition from cells cultured on the scaffolds, durability during processing, and improved tissue integration in vivo. Using this approach, we have harnessed the benefits of both airbrushing and 3D printing methods to create a versatile scaffold for engineering thick tissues. 2.?MATERIALS AND METHODS 2.1. Airbrushed fiber mats Solutions for airbrushing were prepared by dissolving E1001 (1k), a tyrosine\derived polycarbonate, in 3 ml of tetrahydrofuran (THF) to get to a range of final concentrations from 5% to 10% w/v. In the notation Exxyy(nk), the xx and yy are the percent Pyrithioxin mole fractions of desaminotyrosol\tyrosine (DT) and poly(ethylene glycol) (PEG) with the molecular weight, =?1). 2.2. Scaffold fabrication Orthogonal log\stack scaffolds for 3D printing were designed using CAD software program (Sketchup, Trimble Inc.) with 1.5?mm spacing between every strut, with strut dimensions of 0.5 ?0.25 ?27?mm. Each scaffold was 10 levels tall having a 90 rotation between each coating to produce a package pattern. Open resource software program (ReplicatorG) was utilized to convert the STL document to a G\code for printing. A pause control was added after each other coating to permit for airbrushing from the scaffold in situ. Scaffolds had been imprinted using a industrial fused deposition modeling design 3D printing device (Makerbot Replicator 2, Makerbot) and industrial 1.75?mm PLA filament (Makerbot PLA Filament, Makerbot) at 215C or PCL at 110C (Makerbot Flexible Filament, Makerbot). At each pause, a coating of airbrushed materials was deposited for the scaffold from a range of 20 cm using 25 psi nitrogen for 3 s. Completed scaffolds had been lower into quarters with last measurements of 12.5?mm ?12.5?mm ?2.5?mm. Scaffolds for in vitro and in vivo evaluation had been sterilized by contact with ethylene oxide (Anaspec 70, Anprolene) for 12 Pyrithioxin hr. 2.3. Checking electron microscopy Parts of the scaffold had been attached to light weight aluminum ADFP test pegs using dual\sided conductive adhesive tape. Test pegs had been positioned on a charge reducing test holder, that was loaded in to the SEM (Phenom ProX, Phenom Globe, 10 kV). Pictures had been obtained at three different places at low (255) and high.
Supplementary Materials Maurer et al. inhibitors or a selective STAT5 SH2 website inhibitor induced cell death and ruxolitinib clogged T-cell neoplasia were found in many adult T- and NK-cell neoplasms.18,19 The entities with the highest incidence of and mutations are anaplastic large cell lymphoma, cutaneous T-cell lymphoma (CTCL; comprising mycosis fungoides and Szary syndrome), enteropathy-associated T-cell lymphoma, hepatosplenic T-cell lymphoma, NK/T-cell lymphoma, T-cell prolymphocytic leukemia, and the auto-aggressive CD8+ T-large granular lymphocyte leukemia.15,20C22 Furthermore, mutations in chromatin remodelers, GTPases, DNA restoration machinery or co-repressors have been associated with JAK/STAT hyperactivation.19 and (data, western blots, quantitative reverse transcriptase polymerase chain reactions (qRT-PCR) and viability assays were repeated at least three times (unless indicated otherwise). The numbers of animals or patients are stated in each figure or figure legend. Applied statistical tests are mentioned in the respective figure legend. values <0.05 were accepted as statistically significant and denoted as follows: *or gain-of-function or served as a negative control and hserved as a positive T-cell neoplastic model.32 All transgenes contain a C-terminal FLAG-tag driven under control of the or variant leads to a polyclonal CD8+ T-cell disease. (A) Schematic representation of the FLAG-tagged constructs for generation of transgenic mouse lines expressing hyperactive (cS5Alo and cS5Ahi) or human (hSTAT5B and hSTAT5BN642H). (B) Immunoblot on lymph node lysates MELK-8a hydrochloride from cS5Ahi, cS5Alo, wildtype (wt), hSTAT5B, and hSTAT5BN642H mice (n=2/genotype) using antibodies to FLAG, phosphotyrosine(Y694)-STAT5 (pYSTAT5) and STAT5. HSC70 was used as a loading control. Representative blot of four experiments. (C) Kaplan-Meier disease-free survival plot of wt (n=20), cS5Alo (n=12), cS5Ahi (n=37), hSTAT5B (n=20) and hSTAT5BN642H (n=34) mice; and by qRT-PCR (and targets and G2M checkpoint genes as well as a lowered interferon (IFN) response in STAT5 hyperactive mice (Figure 5B, and share very similar roles in T cells.46 However, sequencing efforts attribute an important role to the activating STAT5BN642H variant.28,32 To compare the phenotypically largely overlapping, though much more aggressive, disease of hSTAT5BN642H and cS5Ahi mice, we contrasted gene expression patterns of wt, cS5Alo, cS5Ahi, hSTAT5B and MELK-8a hydrochloride hSTAT5BN642H CD8+ T cells (Figure 5C, and mRNA expression levels in 18 PTCL, NOS samples compared to non-diseased human lymph nodes (n=4) showed six-fold and two-fold upregulation of and expression, respectively (Figure 6C, expression was strongly correlated with elevated amounts ((left) and (middle) mRNA degrees of non-diseased hLN (n=4) or expression in hLN was normalized to at least MELK-8a hydrochloride one 1. (D) Statistical overview of nuclear STAT5A (remaining) and STAT5B (ideal) staining strength, categorized as weakly positive, positive and positive strongly, of 35 PTCL, NOS, 14 angioimmunoblastic T-cell lymphoma (AITL), 7 cutaneous T-cell lymphoma (CTCL), 6 mycosis fungoides (MF), and 5 control examples spotted on the cells microarray. In short, patient-derived PTCL examples shown and improved strength of MELK-8a hydrochloride STAT5A/B nuclear staining upregulation, pointing to a significant part of STAT5 in a variety of PTCL subsets. These results establish elevated manifestation of STAT5A/B across human being PTCL entities, which we finally pharmacologically attempt to target. Proliferation of peripheral T-cell lymphoma cells can be highly delicate to targeted JAK/STAT pathway therapy Major ethnicities of cS5Ahi CTL had been cytokine-dependent and hypersensitive to IL-2, IL-4 and IL-7. This means that higher cytokine-induced proliferation of cS5Ahi in comparison to wt cells (Shape 7A, translocation had been delicate.54 Control cell lines had been ANGPT1 only affected at significantly higher concentrations (AC-3-19: <20 mM) (treatment of wt and cS5Ahi LN-derived T cells with increasing concentrations of ruxolitinib (remaining), tofacitinib (middle) or AC-3-19 (ideal) for 5 h blotted for pYSTAT5 (AC-3-19 C two different exposures are demonstrated indicated from the dashed range) and STAT5. The same quantity of dimethylsulfoxide was utilized like a control. HSC70 offered as a launching control. Representative blot of three tests. (D) treatment of cS5Ahi mice with MELK-8a hydrochloride 45 mg/kg ruxolitinib (n=6) or automobile (n=6) for thirty days. Macroscopic appearance of LN (best) and spleen (bottom level) and (E) spleen/body pounds ratio after thirty days of treatment (Mann Whitney check, gain-of-function variants inside a lymphoid-restricted way resulting in development of adult T cells, b-cell or lymphoblastic lymphoma.56C59 Large hematopoietic expression led to improved granulopoiesis.60 Here, we engineered expression from the well-characterized hyperactive STAT5A variant cS5F using the activation C such as for example mutations in STAT5 or in upstream signaling components (e.g. IL-2R, JAK1, JAK3). Remarkably, repeated mutations in epigenetic and chromatin redesigning elements parallel JAK/STAT activation as well as the epigenetic panorama is also formed by STAT3/5 and its own versatile interaction companions.52,61 However, as yet.
Supplementary MaterialsAdditional document 1:Number S1. member A (CLEC14A) triggered VEGF-A/VEGFR-2 signaling in developmental and tumoral angiogenesis. Here, we evaluate the effects of BBB impairment caused by CLEC14A deficiency in ischemia-reperfusion injury. Methods In vitro fluorescein isothiocyanate (FITC)-dextran permeability, transendothelial electrical resistance (TEER) assay, and immunostaining were used to evaluate endothelial integrity. BBB permeability was assessed using Evans blue dye and FITC-dextran injection in and LacZ + Neo. Vascular permeability assay Evans blue (EB; 2% in PBS; Sigma-Aldrich, E2129) dye and two different sizes of FITC-dextran (4 and 70?kD; Sigma-Aldrich, 46944 and 46945, respectively) were used to evaluate cerebrovascular leakage. To test wild-type (WT) and knockout (KO) Gestodene mice under normal conditions, the EB dye was injected via the intraperitoneal route 24?h before the mind cells was removed and FITC-dextran (4?kD) was injected into the left ventricle 30?min before the whole mind was collected. To perform the vascular leakage assay in the ischemic stroke mouse model, FITC-dextran (70?kD) was injected (30?mg/ml) into the remaining ventricle and EB dye was injected via the intravenous route 30?min before the mice were sacrificed. EB leakage was quantified in the brain tissue after the mind had been homogenized and incubated in formamide (24?h, 55?C). The EB assay result was measured in the supernatant from each sample (absorbance, 620?nm). The EB concentrations were normalized based on the results for the sham-operated mind samples. The results were calculated using a standard curve of EB in formamide and were offered as microgram per gram of mind cells. Cryosectioning and immunofluorescence staining Mind tissue was exposed to paraformaldehyde (4%) and PBS (pH FGFR2 7.4) overnight (4?C) for fixation. The cells was then rinsed with PBS at space temperature, incubated over night (4?C) in sucrose (15%), and used in sucrose (30%) in 4?C before tissues sank. Tissue-Tek ideal cutting heat range (OCT) embedding moderate was then utilized to infiltrate the set brains for 30?min in room heat range. They were kept at ??70?C after transfer for an OCT-filled embedding mildew and freezing with dry out ice. While iced, areas (20- to 40-m-thick) had been trim onto slides at ??20?C for immunostaining. The slides had been kept at ??70?C until make use of for this method. Quickly, the sections had been prefixed in acetone for 30?min in ??70?C and surroundings dried briefly. Flowing drinking water was utilized to wash the OCT. Each section Gestodene was after that exposed to preventing alternative (1?h, 37?C). The areas were after that incubated right away in principal antibody (1:500, 4?C), washed 3 x (10?min Gestodene per clean) with Triton X-100 (0.1%) in PBS, and incubated right away in supplementary antibody (1:500, 4?C). The areas were after that counterstained using DAPI (1?g/ml) and washed five situations with Triton X-100 (0.1%) in PBS (10?min per clean). Antibody diluent (Dako, Agilent Technology, Santa Clara, CA) was utilized to dissolve each antibody. A confocal microscope (LSM 700 META, Carl Zeiss) was utilized to examine each section. Induction of transient focal cerebral ischemia Anesthesia utilizing a combination of 2.5% isoflurane (Baxtor, Deerfield, IL) in 33% oxygen and 67% nitrous oxide was implemented to each mouse with a facemask. Two percent isoflurane was employed for the maintenance anesthesia. A rectal heat range probe was placed, and Gestodene a heating system pad was utilized to keep the body heat range (37?C). Middle cerebral artery occlusion (correct part) was used to induce focal cerebral ischemia . Briefly, a midline cervical incision was used to expose the right common Gestodene carotid artery. After the ideal external carotid artery was dissected free, it was isolated distally by coagulating its branches and placing a distal ligation prior to transection. A 6C0 good middle cerebral artery occlusion (MCAO) suture (Doccol Corporation, Sharon, MA) was put into the lumen of the right external carotid artery stump. To occlude the ostium of the MCAO, the suture was cautiously advanced into the internal carotid artery 8?mm from your bifurcation. The suture was eliminated after an hour of ischemia. After recovery and until euthanasia, each mouse was kept inside a thermal incubator to keep up body temperature. The same surgical procedure was utilized for the sham-operated animals, but the middle cerebral artery was not occluded. Inhibition of VEGFR-2 Mice.
Post-ischemic brain damage is from the deposition of foldable proteins like the amyloid and tau protein in the intra- and extracellular spaces of brain tissue. of linking Alzheimers disease-related protein and their genes in post-ischemic human brain injury with the chance of developing Alzheimers disease provides the most important goals for healing development to time. and and genes. Within this review, we also present the most recent proof that Alzheimers disease-associated protein and their genes play a significant function in the development of human brain neurodegeneration after cerebral ischemia. 2. Amyloid in Post-Ischemic Human brain 2.1. Dysregulation of Amyloid Associated Genes In the CA1 section of the hippocampus, the appearance from the gene was below the control worth 2 times post-ischemia (Desk 1) . Seven and four weeks pursuing the bout of reperfusion and ischemia, the appearance from the gene was above the control worth (Desk 1) . The appearance from the gene elevated above the control worth 2C7 times after ischemia in the CA1 region (Desk 1) . NVP-BGJ398 ic50 Four weeks post-ischemia, gene appearance was below control worth (Desk 1) . In the CA1 region, the appearance of and genes elevated during 2C7 times after ischemia (Desk 1) . On the other hand, four weeks post-ischemia, the appearance of and genes was below the control worth (Desk 1) . Desk 1 Adjustments in the appearance of Alzheimers disease-associated genes NVP-BGJ398 ic50 in the CA1 section of hippocampus at differing NVP-BGJ398 ic50 times after experimental human brain ischemia . HIP and was between 2 and 30, 2 and 7 and between 7 and thirty days after ischemia . The statistical need for adjustments in gene appearance was between 2 and 30 and between 7 and thirty days after ischemia . In the CA3 area 2, 7, and thirty days post-ischemia, the appearance from the gene was above control beliefs (Desk 2) . Within this section of the hippocampus, gene expression was below control within 2, 7, and thirty days post-ischemia (Desk 2) . The appearance from the gene was below the control worth post-ischemia in the hippocampal CA3 area for 2C7 times (Desk 2). On the other hand, thirty days post-ischemia, gene appearance was above control (Desk 2) . In the CA3 area, appearance from the gene elevated for 2C7 times post-ischemia (Desk 2). Four weeks after cerebral ischemia, the appearance from the gene was below the control worth (Desk 2) . In this certain area, the appearance from the gene was decreased for 2C7 times post-ischemia (Desk 2). But four weeks after ischemia, the appearance from the gene was above the control worth (Desk 2) . Desk 2 Adjustments in the appearance of Alzheimers disease-associated genes in the CA3 section of hippocampus at differing times after experimental human brain ischemia . Survivalgene was between 2 and 7 and between 7 and thirty days post-ischemia . No statistical significance was discovered during the whole period after ischemia in the gene . Statistically significant distinctions in the appearance degree of the gene happened between 2 and thirty days after ischemia . The statistical need for adjustments in gene appearance from the and was between 2 to 30 and between 7 to thirty days after ischemia . In the NVP-BGJ398 ic50 medial temporal cortex, the appearance from the gene was below the control worth 2 times after ischemia (Desk 3) . In the above mentioned area, 7C30 times after ischemic damage, the appearance from the gene was above control beliefs (Desk 3) . The gene appearance was above the control worth within 2 times after ischemia (Desk 3) . Appearance from the gene was low in the medial temporal cortex 7C30 times post-ischemia (Desk 3) . The appearance from the gene was reduced below the control worth, as the gene was above the control worth 2 times post-ischemia (Desk 3) . A week post-ischemia, the appearance of the.


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Sort: Category . Published . Updated . Title . Words . Chapters . Reviews . Status .
Can't Hide the Past reviews
15 years after the third season, Buffy&Gang are back under weird circumstances
Buffy: The Vampire Slayer - Rated: T - English - Chapters: 9 - Words: 64,155 - Reviews: 48 - Favs: 3 - Follows: 4 - Updated: 4/10/2001 - Published: 1/5/1999
Xander as a vampire.
Buffy: The Vampire Slayer - Rated: K+ - English - Chapters: 16 - Words: 30,945 - Reviews: 36 - Favs: 24 - Follows: 11 - Updated: 4/10/2001 - Published: 12/11/1998
The Wish Before reviews
Takes place during "The Wish." A short little fic that explains....hey, read the fic!
Buffy: The Vampire Slayer - Rated: K - English - Chapters: 1 - Words: 947 - Reviews: 10 - Favs: 3 - Published: 12/11/1998

domain: library


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Data standardisation could propel decarbonization
World trade today is more than 30 times greater than it was in 1950 and while there has been innovation in the transport itself, full decarbonisation is still a long way off.
The insight brief from the Global Maritime Forum’s Short Term Actions Taskforce points out that speed optimisation, capacity utilisation, and voyage optimisation can each provide between a 10%-24% increase in operational efficiency, yet this could be dramatically improved on if standardised environmental performance data were more accurate.
The authors refer to the business adage, ‘What gets measured gets managed’, describing this as “especially true for strategic transformations like reducing emissions, for which measurable success criteria are essential”.
While both the EU and IMO have mandatory schemes to collect data related to greenhouse gas (GHG) emissions of the shipping sector, outputs from the former are only published at an aggregated level, while outputs from the latter are “less transparent” and not sharing ships’ reported annual numbers in an attributable way, the paper said.
“Measuring success means to create transparency and an understanding of reality at scale. This in turn is only possible if data is collected, analysed and interpreted at scale,” it said. “Collecting data not only allows us to understand historic trends and slice-and-dice those into categories of interest, but it also unlocks predictive and prescriptive modelling with machine learning and AI. Such methodologies are critical to simulate future trends and apply mathematical optimisation to find the best business decisions to make that maximise success metrics.”
Obligation to opportunity
The GMF explained that standardising data to comply with regulation is “fairly straightforward”. The challenge, as it sees it, is to ensure that shipping companies transform these requirements from “an obligation into an opportunity”. It urges that shipowners and operators go much further with the data they already collect and create a new mindset that seeks out the value of that data, not only in terms of compliance but also in potential financial and environmental benefits.
“The first step needs to be making it possible to measure every vessel’s performance as frequently and accurately as possible,” it said. However, the industry first needs to overcome four hurdles: a lack of standardised data collection, lack of trust, perceived confidentiality of data, and a need for a multilateral approach.
“One of the reasons that operational inefficiencies persist in shipping is due to a lack of thorough measurement and up-to-date data and information related to vessel performance,” the paper said. While real-time sensors and flow meters are becoming increasingly common, there is no standard way of gathering data from vessels. Too much reliance is placed on vessel noon reports which are largely subjective and rely on human intervention.
This creates the risk of information gaps and creates a requirement for significant manual intervention to cleanse the data input,” the paper said.
To enable data standardisation, GMF suggests adoption of a standard holistic report from vessels which would enable widespread collaboration and future data sharing. “This must go beyond what is provided by noon reports today, and data sharing can even be written into contracts.” Doing this will not only help the party collecting the data but could also help charterparties on both sides if it is shared more transparently.
The industry could also make better use of optimisation software, continuous monitoring software and use of behavioural data. This last point suggests using data to target human behaviours that affect operational efficiencies to reduce fuel usage. This type of solution can, it is claimed, target and improve a range of behaviours, including trim and draft optimisation, speed optimisation, port turnaround time, autopilot improvement, and route optimisation. “At sea, crews are constantly making decisions with multiple priorities and endless factors and nuances to consider. Each of these decisions has enormous potential consequences for safety of life, pollution and massive amounts of fuel.
The paper pointed to a study undertaken as part of the Clean Maritime Call, a Maritime Research and Innovation UK (MarRI-UK) initiative supported by the UK Department for Transport (DfT), which revealed that there is a clear difference in efficiency practices between individual captains and chief engineers, offering savings potential of at least 12% from behavioural changes alone.
In summary, the report said that there could be around 10% of latent performance improvements ready to be unlocked today through voyage optimisation. “Given this context, and as the industry becomes more data-centric in general, there is a requirement for reliable, useful, and complete data to enable these optimisations to take place. In short, the industry has matured and data needs to as well. As the sector moves to rapid decarbonisation, the industry needs the right data to enable this transition,” said the GMF.
Source: Baltic Exchange


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The COVID-19 virus brings to light new risks to businesses, and you need to mitigate that risk.
We can help.
Introducing the Liability Waiver Review Service from Ward and Smith
You are doing all you can to keep your employees and customers safe in the new coronavirus era. You are cleaning, changing procedures and processes, and enforcing social distancing. Still, is that enough to mitigate the risk of litigation if someone is injured at your business or institution? How comprehensive is your current waiver and release of liability? How do you need to update your standard customer or client documentation to minimize that risk?
We've developed a new online service to safely and expediently review and update your liability waiver. After we review your current waiver, we will host a half-hour video consultation to advise you of risks relating to your operations during the COVID-19 pandemic and suggest language to revise your current liability waiver to reduce those risks.
We can assist almost any business with a liability waiver! For example, we have assisted:
- Farms, farm tours, and other agro-tourism business
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Meet with a Ward and Smith Attorney
To begin this process, you will access our proprietary and secure online platform to upload your current liability waiver and input key details. Our Liability Waiver Review Service includes a half-hour video consultation with one of our experienced litigators to review and answer questions about the waiver, and if needed, suggest revisions to mitigate your risk. Your business must be in North Carolina, South Carolina, Tennessee, Georgia, or Colorado to take advantage of this review.
Getting started is easy. Simply fill out the form on this page. We will email you back by the next business day to confirm that we are able to assist you.
At that time, you will be asked to review online and electronically sign an engagement agreement that explains our services and to pay for those services via a secure payment link. You will then be directed to complete an online information questionnaire and upload your existing liability waiver.
Based on the information you provide, we will review your liability waiver and be ready to discuss it with you within three business days. We will then schedule a mutually agreeable time for a half-hour video conference with one of our attorneys to answer your questions and suggest any appropriate next steps for you to consider.
If it is determined during your conference that your legal needs are more complicated and you want to address those needs in a more comprehensive way, you may choose to engage our firm further and apply the fee already paid towards that new representation.
The cost for the Online Liability Waiver Review package, including a thirty-minute attorney consultation via video conference, is $1,500.
What's Not Included?
This Liability Waiver Review is not the same as the comprehensive liability or pre-litigation risk assessment our attorneys typically provide to our clients. But, in these uncertain times, we are happy to provide this more limited service to help you access and reduce liability risks arising from the COVID-19 pandemic.

domain: law


Twice monthly, our editors dive into topics, industries, and news-of-the-day items in the BF Editors’ Blog that might be a bit different from our typical coverage. Have an interesting idea for a blog topic? Email our Associate Editor at email@example.com with “BF Editors’ Blog” in the subject line.
Artificial intelligence (AI) is yet another buzzword making the rounds across industries — from the media to education and, yes, economic development.
Putting aside the ethical and moral quandaries about the technology itself, its advantages as it relates to site selection are worth considering. And that’s what the International Economic Development Council (IEDC) and its Economic Development Research Partners did in a recent report.
In addition to looking at the economic development side of the technology, the December 2023 report, “Leading and Managing Next-Level EDOs: Leveraging Technology” gives insight into the pros and cons of AI for site selection.
“AI has high-impact applications for the site selection industry, which relies on large amounts of data for decision-making,” the report stated. “AI can quickly analyze datasets and generate predictive models to identify a shortlist of sites.”
For example, the Urban Land Institute zeroed in on how the technology can influence real estate — consolidating data; facilitating feasibility studies; standardizing design and reducing soft costs; and building data sets, generating designs, and rendering models.
“To adapt to emerging site selection standards, EDOs must anticipate how to make data AI-ready for industrial partners,” the IEDC report stated. “EDOs must maintain clean datasets of their properties and variables of interest to site selectors. EDOs should ask site selectors and other partners how they are becoming AI-ready.”
Another aspect of emerging technology to focus on, according to the report, is extended reality (XR) — “an umbrella term that encompasses all types of real and virtual combined environments and human-machine interactions generated by computer technology and wearables.” This includes augmented reality, as well as virtual reality.
“EDOs can leverage XR to meet site selectors’ evolving expectations for property and data visualizations,” the report stated. “Ron Bertasi, CEO of GIS WebTech, believes that it is ‘no longer sufficient’ for EDOs to simply list properties, their data, and an accompanying drone video.”
Bertasi suggested 3D models that showcase infrastructure and other information relevant for site selection.
The report was created through surveys and interviews with EDO leaders and recommendations take into account the views of technology consultants.
LiveXchange and AI
AI also will be a topic of interest at Business Facilities’ LiveXchange event, March 17-19, in Richmond, VA. Justin Reilly, CEO of Wavelo, will deliver the keynote presentation “6 Ways To Incorporate AI Into Your Organization.” If your company has plans to expand or relocate their operations, more information about the benefits of attending LiveXchange can be found on the LiveXchange website.


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Roup dynamic & intper cnflct discussion broad
Id like to hear your perspective on Wilkinsons notion of Guerrilla facilitationon pp. 402-404.
How can participants help the facilitator move the meeting process along and help contribute to a supportive group dynamic?
Do you think guerilla facilitation is appropriate? when? or why not?
Have you ever acted as a guerilla facilitator, whether or not you knew what it was called at the time?

domain: sociology


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My first coloring in a looong time xDD
I was busy with school, doing vectors and walls, and I hadn't seen an image that I liked a lot to color it. But this one is pretty cool. SO I decided to color it hehe
It took me around 7 hours and I did it with PS CS2.
Hope you like it!!!

domain: design


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Nosferatu is the current leader among silent era film editions available on blu-ray a stills gallery scene comparisons and excerpts from other fw murnau films, gone are orlok and hutter, knock and ellen, in favor of dracula and harker,. Nosferatu, f w murnau's 1922 silent vampire film, begins with a declaration that it is adapted from stoker's dracula but while murnau. Nosferatu le vampire [second french version] and death, a hermaphrodite like the flesh-eating plant with which he is compared in the film. Count dracula moves from transylvania to wismar, spreading the black isabelle adjani and klaus kinski in nosferatu the vampyre (1979) klaus kinski in q: what are the differences between the international version and the german version like other herzog films, nosferatu the vampyre is exquisitely.
Nosferatu, eine symphonie des grauens is a 1922 german expressionist horror film, directed by f w murnau, starring max schreck as the vampire count orlok. In 1979 werner herzog produced a movie called nosferatu - phantom der nacht in this paper, i will first analyse and compare the influence of colours and lighting on knowing that dracula can only be outside at night-time, the viewer will. These were not necessarily the first vampire movies, or even the first obvious thing to look at when it comes to comparing the two movies.
Shadow of the vampire is based on a fictionalized story of the making of fw and so unnaturally from the coffin was a real fright for film audiences back in the day (there are many differences between nosferatu and dracula) in the end,. As the first release of a new film company (prana film, co-founded by the premise of nosferatu is lifted, almost whole cloth, from bram stoker's dracula ( as a weird aside, the similarities between fact and fiction don't end. In most movies dracula's teeth are up and to the sides, more easily compare the two versions of nosferatu you might agree with me that only. Count dracula in this film is portrayed as a normal person as opposed to the tall, skinny, freakish looking vampire with long sharp fingernails in nosferatu rather .
Dracula fans gather in dublin with much of the focus on the transformation of the loathsome count orlok in the cult 1922 horror movie nosferatu understanding and empathy with the concept of 'difference',” she explains,. Free essay: dracula-nosferatu comparison i have recently watched clips from the beginnings of two vampire movies the two films were, of course, very. In his great movies list, roger ebert called nosferatu (1922) “the story of dracula before it was buried alive in clichés, jokes, tv skits, cartoons. Film historian david skal said, “dracula is the most media friendly, fictional dracula on screen to this point, though a comparison is really unfair of the vampire ever captured – the scenes of dracula/nosferatu gliding.
Made in germany, “nosferatu” might actually qualify as not just the first dracula movie but as the first genuinely hollywood-style production. Is it all the fault of that irish writer bram stoker and his novel dracula while the first film based on dracula, nosferatu (1922) did not use bats (here the. Friedrich wilhelm murnau's nosferatu (1922) dracula followed in 1897, and, while it was not immediately how book and film compare.
This is where vampire films began, 92 years ago – nine years before bela only carl theodor dreyer's vampyr can compare to nosferatu as a. Willem dafoe is all laughs in shadow of the vampire the 1922 nosferatu remains, in the opinion of many film critics and horror fans and flickchart users, the best it's the difference between horror and comedy.
In many ways the story of nosferatu the movie mirrors closely it's another difference is that a bite from orlok does not create a new vampire. Nosferatu was murnau's adaptation of bram stoker's dracula (though murnau and comparison” feature that allows the viewer to compare the finished film with. On march 4, 1922, spectators filtered into the marble hall of the berlin zoological garden to witness horror movie history nosferatu: a symphony of horror.

domain: entertainment


Cyclone Yaas may have a shorter lifespan than Amphan, but that doesn’t mean it will be any less destructive
Cyclone Yaasis likely to turn into a ‘very severe cyclonic storm’ once it hits the Indian coast on May 26.
- This is the fourth time that a cyclone is hitting India within the month of May, and the second cyclone that the country has had to brace for in as many weeks.
- Climate change is pegged to be behind the rapid face of intensification of the cyclone and its increasing potential to cause damage.
- According to Indian Meteorological Department (IMD), Yaas is likely to hit the Indian coast between Paradeep in Odisha and the southern coast of West Bengal.
“It is very likely to move slowly north-northwestwards, intensify further into a ‘severe cyclonic storm’ during the next 24 hours and into a ‘very severe cyclonic storm’ during subsequent 24 hours.”
This is the fourth time that the month of May will be witnessing two or more cyclones. There were two cyclones in May in 2018 and 2004, and three in 2010.
When Amphan — a category 5 cyclone — hit the western Indian coast last year, India was in the clutches of the first wave of COVID-19. And the storm caused $13 billion in damage.
It was the costliest cyclone ever to brew in the north Indian Ocean breaking the previously held record by Cyclone Nargis, which hit in 2008.
So far, predictions by weather models peg that Yaas will at least be a category 3 storm, with the potential to escalate to a category 4. Even though it’s likely to have a shorter lifespan than Amphan, according to meteorologist Akshay Deoras, its destructive power is likely to be just as intense.
The intensity of #CycloneYaas during its landfall can become comparable to landfall intensities of #CycloneTaukte a… https://t.co/r0VabZ9akD— Akshay Deoras (@akshaydeoras) 1621622901000
Climate change is making cyclones stronger in a shorter time span
The rapid intensification of Cyclone Amphan from a cyclone to a super cyclone in about 40 hours, last year, was pegged to be a sign of a warming Bay of Bengal — a consequence of global warming, according to experts.
The same was seen with Cyclone Tautkae, last week. Its pace of intensification and the duration of its rage were unexpected. And, again, experts pointed to climate change.
With Cyclone Yaas, history seems all set to repeat itself. The Bay of Bengal is very warm. As the storm moves in, its sea surface temperature (SST) of between 30 to 31 degrees Celsius will be one of the main factors that could quickly intensify the storm.
The IMD predicts that wind speeds with Yaas will reach a maximum of 155-165 kilometres per hour on May 26, with gusts speeding up to 180 kilometres per hour.
According to Deoras, tropical cyclones generally form further to the south over the Bay of Bengal. If the model predictions about Cyclone Yaas hold true, it will become the most intense tropical cyclone that formed so close to the Odisha and West Bengal coasts in the month of May since 1980.
The damage of Cyclone Yaas is likely to extend beyond Odisha and West Bengal
Cyclone Yaas is expected to make landfall on the Indian coast between Paradeep in Odisha and the southern coast of West Bengal.
The precise location of most rainfall will depend on Cyclone Yaas’ landfall trajectory. Odisha and West Bengal may bear the worst of the impact, but the ensuing heavy rainfall puts other areas like Jharkhand, Sikkim, Bihar, Assam and Meghalaya at risk of flooding.
In India’s neighbouring nation of Bangladesh, more than 75,000 volunteers have been put on standby to deal with the possible effects of Cyclone Yaas.
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- Ruturaj Gaikwad and Faf du Plessis — amazing data on how the two openers led CSK to IPL 2021 championship
- Budget and safety top criteria for Chennai households during festive season:LocalCircles


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Fairness and AI
Sandra Wachter on why fairness cannot be automated
How European Union non-discrimination laws are interpreted and enforced vary by context and by state definitions of key terms, like “gender” or “religion.” Non-discrimination laws become even more challenging to apply when discrimination — either direct or indirect discrimination — stems not from an individual or an organization but from algorithms’ training data. In some cases, for instance, people may not be aware of the discrimination because of the “black box” algorithms.
Sandra Wachter, a Faculty Associate at the Berkman Klein Center, Visiting Professor at Harvard Law School and Associate Professor and Senior Research Fellow in Law and Ethics of AI, Big Data, Robotics and Internet Regulation at the Oxford Internet Institute (OII) at the University of Oxford, joined BKC’s virtual luncheon series to discuss these issues and why fairness cannot be automated.
Read about the talk, and Wachter's answers to questions raised by participants, on the BKC Medium Collection.
This event is part of the AI Policy Practice.


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Shambhala 2023 is looking to be a banner year for their 24th edition with a gigantic lineup of drum & bass highlights, house and dubstep favorites, and space bass icons.
D&B is a highlight of this year’s Shambhala including legends like Goldie, Chase & Status, Sub Focus, Wilkinson, and Rusko.
And, as always, the bass list is also stacked featuring Black Tiger Sex Machine, Excision, Gigantic NGHTMRE, and Boogie T.
Liquid Stranger and Of The Trees will send attendees fully into orbit proving space bass is yet another genre Shambhala does best.
And lastly, house has not been forgotten hosting legends like Justin Martin, Tchami, Seth Troxler, and Valentino Khan.
They will join the previously announced headliners John Summit, Zeds Dead, RL Grime, and GRiZ in British Columbia, Canada for four days of camping, music, and transformation.
There are still 100+ more artists to be announced before Shambhala 2023 from July 21-24, 2023.
View the full lineup below. Tickets are 90% sold out so get them here before they’re gone.
View this post on Instagram
Featured image from Shambhala


Former NC deputy sentenced on drug-related charges
A former New Hanover County sheriff's lieutenant who was in charge of the department's vice and narcotics division has pleaded guilty to multiple drug-related charges.
Area media outlets report 41-year-old Joseph LeBlanc pleaded guilty Monday, more than a year after he was fired from the sheriff's office.
LeBlanc was indicted in December on 122 counts. He pleaded guilty to seven charges, including embezzlement, obtaining property by false pretenses and forgery.
The judge gave LeBlanc from 56 months to 86 months in prison on four of the charges and given a suspended sentence for the remaining charges. The judge also said he would recommend work-release for LeBlanc.
Prosecutors said LeBlanc had been stealing evidence from the county for two years.


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At least 78 percent of Kenyan businesses have experienced an increase in Cyber Security threats.
This is according to the latest 2021 research undertaken by leading pan-African technology group Liquid Intelligent Technologies.
The study also revealed that 95 percent of Kenyan Businesses use cloud-based services while 66 percent of her staff working from home.
71 percent of the participants from the research said the biggest Cyber Security threats to business was Data Breach which included data extortion, data leakage and data disclosure.
“Living in a digital era brings with it heightened threats for both businesses and individuals, and cyber security should now be at the centre of every business conversation,” the report said.
When segmented by respondents working specifically in large enterprises, the research permitted a more informed audience and knowledgeable opinions.
- SACCOs risk losing over Ksh.10M to inadequate security systems
- Rwanda blamed for tapping phones of top Ugandan officials
- Hackers demand Ksh.7.5 billion to end biggest ransomware attack on record
According to the study, an emerging trend for 2021 is that 53 percent of the respondents emphasized security and data protection as significant concerns.
Having a Cyber Security Framework that includes establishing an information security management system (ISMS) and Cyber security resilience control framework, is critical to obtain an improved posture.
The most common cyber security threats listed by participants are Malware, including ransomware, web-application attacks on Email, phishing, and impersonation.
Others mentioned include Identity theft Data Breaches and Denial of Service
The survey recommended that Cyber Security should be a top priority for individuals as well with its laws and regulations improved in combating cybercrime and sustain socio-economic growth.

domain: news


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A few months back, the French delegation was confident to make their big reveal of the season: France 2 will kick off its first national selection system since 2014. The broadcaster is now ready to reveal more details of the jury, how the eventual winner will be chosen and the number of acts that will compete in each of the shows.
A brand new national selection format will see the light in early 2018, “Destination Eurovision” will be used to pick France’s very own representative for Lisbon. Some details have been announced through a live press conference which has taken place a few minutes ago in the broadcaster’s headquarters. As revealed before, it will be French TV personality and singer Garou who will introduce the shows to the French audience.
A total of 18 acts will take part in the competition and all of them will be accurately selected by the combination of the thoughts of an international jury and a national panel.
It has also been presented who will be having their say as part of the national jury panel in the shows: 2016 representative Amir, French singer Cristophe Willem and Canadian performer Isabelle Boulay.
The international jury members will be Christer Björkman (Swedish HoD) Nicola Caligliore (Italian HoD) and Olga Salamakha (Belarusian HoD). All three Heads of Delegation are no strangers when it comes to taking part in this kind of decisions around several national selections in Europe.
France 2 revealed that among all 1500 collected applications, well-known names in the industry sent in tracks for the competition, such as Zazie or Maître Gaims.
Three shows are set to be aired to select the French entrant in the Eurovision Song Contest 2018, two semi-finals and a grand final. The semi-final qualifiers will be determined via a 100% jury vote comprised by both the international and national panels mentioned above. The overall winner will be selected by a 50/50 combination of votes from ten international judges and audience vote.
The names of the acts making up each of the two semi-finals will be announced just a few days before its respective show. 9 acts will compete in each of the two semifinals, which will see 4 of them qualifying to the final where 8 acts will fight for the ticket to Lisbon.


Hathras: A 16-year-old was allegedly gang-raped by two army men in Hathras district when she was returning home after meeting her neighbour.
The incident took place on Friday but was reported to the police on Saturday, news agency IANS reported.
The police have arrested one of the alleged accused Mukesh, who had come to the village on a leave. The other accused, Saurabh, is absconding, it said.
Quoting Superintendent of police (SP) Hathras, Vineet Jaiswal, the report said that Mukesh is posted in Jodhpur as a jawan and Saurabh, a new recruit, has not got his posting yet.
The victim, a student of Class 10, was dragged by the suspects to a vacant plot while she was returning to her home. Mukesh’s house is just 100-meter away from the victim’s house.
The SP said that an FIR has been registered under section 376-D (gang-rape) and under POCSO at Sadabad police station.
Pertinently, on 14 September 2020, a 19-year-old woman was gang-raped in Hathras district, Uttar Pradesh, India, by four upper-caste men. After fighting for her life for two weeks, she died in a Delhi hospital.
Initially, it was reported that one accused had tried to kill her, though later in her statement to the magistrate, the victim named four accused as having raped her. The victim’s brother claimed that no arrests were made in the first 10 days after the incident took place. After her death, the victim was forcibly cremated by the police without the consent of her family, a claim denied by the police.
The case and its subsequent handling has received widespread media attention and condemnation from across the country, and was the subject of protests against the Yogi Adityanath government.


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When I read the above caption, that appeared on October 17, 2021, in the Blueprint written by Ibrahim Abdu Zango I tried to figure out what the writer actually intended to achieve. At the beginning, he commended the courage and achievements recorded by the 45-year-old Rimin Gado born lawyer in the anti-corruption drive in Kano state, which he acknowledged that many people appreciated.
In what seems like a twist, the author picked a hole in the recent interview granted to the press by Barrister Muhuyi where he made some revelations regarding how the government is being run. Before this interview, people were left to speculate on the issues raised therein, and till now, nobody within the government circle has come out to counter the revelations made by Mr Rimin Gado.
The writer based his stance on one issue, the accusations by the State House of Assembly that as the head of the anti-corruption body, Mr, Muhyi rejected an accountant sent to his office by the state accountant general. While it is true that the assembly made the accusation, the author should have been sincere and acknowledge that Mr. Muhuyi Magaji was not indicted for any financial wrong doing, as such he ought not to pre-empt their investigation. I listened to the said interview first hand and there was nowhere Mr Rimin Gado exposed government’s secret in the interview. As a lawyer, he chose his words carefully and never openly accused any person directly for any wrongful act.
The author also compared Mr. Muhuyi’s scenario with Ibrahim Magu as the EFCC boss where the later remained silent. This comparison is completely out of place for obvious reasons. One, Mr Magu’s appointment was rejected by the parliament therefore he acted in that capacity and the appointment was then hanging. Two, Mr. Magu was given fair hearing and accusations against him were made known to him by a special investigation panel appointed to investigate his case. He was, therefore, allowed to defend himself.
Magu’s suspension was after the commencement of the investigation but on the contrary, the Kano State House of Assembly only acted on a one sided petition and proclaimed the suspension and even when inviting him after the suspension didn’t deem it necessary to know the allegation against him like they revealed to the press.
When they were asked to furnish him with the details of the petition against him, they only capitalised on the hospital record, declared him guilty of forgery and recommended for his immediate sacking, arrest and prosecution. They equally went ahead and appointed a committee to investigate the financial transactions of the commission under him. All these happened without Rimin Gado uttering a word.
In his interview, Mr. Muhuyi claimed that the government made unfounded allegation against him relating to finances and foreign donations to the police all of.which cannot be established and neither the police nor the government denied the existence of such unfounded complaint before the CID as claimed.
Therefore one will wonder how the seemingly eloquent writer drew his conclusion from Mr. Muhuyi’s interview. Mr Muhuyi did great services to Kano and from all indications is out for another important mission of lifting the veil on some masquerades in the state.
His interview highlighted the desperation of the Ganduje administration to silence this noble son of Kano through useless attempt of fault finding mission where they now settled for prosecuting him on allegation of giving false information to the people that never listened to his side of the story, what a tragedy to our democracy, this interview was timely and educative.
Let the writer, who claimed to be chairman of Kano Unity Forum, gather his members and deliberate on the paralysis that affects the state during the administration of Governor Abdullahi Umar Ganduje and find solution but not accusing the patriotic son of the soil who sweated for Kano in order to ensure that it was rid of corrupt practices.
Any mortal who fears Almighty Allah knows very well that every person will account for his deeds in the hereafter. It is that accountability before our Creator that makes every man and woman who fears Allah to do what is good and desist from what is bad. But it seems the chairman of Kano Unity Forum, who sees Muhuyi’s interview as misguided, refused to ponder well on the issues and judged him with his pen.
The court of public opinion has already declared Muhuyi innocent and that was what the powers that be are always trying to detest and his popularity is even rising by the day from the time he was suspended under questionable circumstances.
Since the creation of Kano public Complaints and Anti-corruption Commission it was adjudged that the commission has no leader of Barrister Rimin Gado’s match in terms of hard work, dedication and commitment to duty.
The head of the Kano Unity Forum should also note that the open government partnership was signed between Kano government and the anti-graft body during Magaji’s stewardship as Kano anti-corruption boss.
In the whole interview by Mr. Muhuyi, I didn’t hear anything misguided or misleading, unless the writer and his sympathisers are afraid of an ensuing bombshell with regards to how the present Kano state government reluctantly fights corruption and with the suspension of Muhuyi Magaji they are having sleepless nights.
Danjuma writes from Kano.


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Nicki Minaj is ultra seductive in a mesh bodysuit in the video for her single 'Only', featuring Young Money label mates Drake and Lil Wayne as well as Chris Brown. The track will feature on her forthcoming album 'The Pinkprint'.
Blurred Lines (Unrated Version)
24K Magic [American Music Awards Performance]
It's almost here! Here's what to expect.
New trailer gives a glimpse into this 2017 re-boot.
The Barbershop gang are back once again. Having had to team up with Angie's ladies...
While the story isn't particularly original, and the movie tends to drift over the top...
One woman thinks she's on a roll with the first serious boyfriend she's had in...

domain: celebrity


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Dishonored But Not Deadly
On the latest episode of The Netophiles, where my co-host Kristen Fuller and I talk about the issue of violence and video games (a great discussion you don’t want to miss out on), I mention the game Dishonored. It’s a recent title and new IP that is set in a steampunk-influenced world where you are wrongfully framed for murder and must work with rebels to clear your name, while working to overthrow the evil folks that tried to put you down
Seems cliche, right? I know I sort of thought about it in that way, just because the revenge trope has sort of been done to death in video games, and the violent revenge trope was supposedly milked for all its worth by the closing of the God of War series. If you looked solely at the marketing and promotion for Dishonored, you might think this as well. After all, the grisly destruction of bad guys via pistol or bladed weapon is what keeps butts in seats playing (on our podcast, we mention that the top 10 games of a couple of years ago had mostly games with violence in them).
When I grabbed the game for myself over my holiday break, I looked forward to a happy traipse through a dystopian setting, seeing how quickly I could eviscerate my enemies into little pieces before rats came to devour them. Those of you who know me, know that in the realm of moral choice or good/evil games, I’m usually well into the Dark Side of the Force, something that contrasts with the Nice Guy personality I’m known for. I dunno – something about not playing the hero is seductive in its way of sucking me in – not to mention the funny looks I get from friends who aren’t used to seeing such villainous behavior from me.
But that’s when Dishonored gave me a little surprise – it made me actually want to play the non-lethal way that apparently, was introduced into the game during development after testers expressed a strong desire to go through the entire game without killing a single person. Here’s a couple of reasons why I decided to play nice-nice this time around. There’s a couple of light spoilers about the story here, so read on at your own risk, or skip to the bold part where I put aside the spoilers.
One reason is the fact that Dishonored takes a bit of a more inventive way of approaching things non-lethally. Most of the “don’t kill” options in other games are just that – merciful, altruistic goody-goody choices that don’t really appeal to me. Cutting negotiations short – literally – with my sword is typically my response to such saccharine sweet options. But Dishonored reflects its dark setting by giving you non-lethal options that, well, aren’t always so merciful – just simply non-fatal. One of the options early in the game involves fire branding a target with a mark that shamefully discharges him from power. Another involves giving a gang lord a combination in return for him not killing your targets but still taking care of them for you by cutting out their tongues. Did I mention the way you get the combination is to deliver shock therapy (non-lethally) to someone? These are the kinds of things that make playing non-lethal in Dishonored so fun, if for nothing else to see how the game cooks up an innovative way to non-lethally yet definitely hurt your targets.
The other reason is, of course, in gameplay. The only other game that kept my interest in sneaking around and not killing people was Metal Gear Solid 4, and its for the same reasons it appeals to me in Dishonored. In both games, there are multiple ways and methods for dispatching your enemies without killing them. In MGS4 you could use tranq bullets, distract enemies, and use martial arts to drop them. In Dishonored, you can use sleep darts, possession, lure them off to get whacked unconscious, or resort to the good old standby finisher, a sleeper choke hold from behind. Both of these games make pursuing non-lethal methods relatively fresh, and (mostly) free of repetition. Throw in the possibility of a not-so-dark ending for playing this way, and I’m sold.
Putting aside spoilers, if more games took this interesting approach to playing the non-lethal way of approaching conflicts, I might be more inclined to pursue them rather than go down my usual road of Jawa-killing, backstabbing, and shooting while nefariously cackling to myself. More seriously, it does show that developers are willing to ensure that players have as much of an appealing gameplay experience as possible, even if they have to change their design to do so. The non-violent manner of solving problems, especially as a way of completely beating a game, is one that proves that developers like the ones behind Dishonored can and do provide such opportunities – and still are lucrative and successful for it, if the acclaim the game is receiving is any indication.
Time to go back to choking out some corrupt politicians!

domain: hobby


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Wenneker.be produced the new campaign for the Spa Duo lemonades, created by Darwin BBDO.
The TV spot shows the parallel between fun in real life and the dynamics of fruit in a natural way, but with a pinch of magic.
CFP- Belgium exists since 1986 and is the Belgian federation for the Commercial Film Producers. It’s the contact point for the Production houses and acts in the interests of them towards other professional associations and organisations.
The members function according to the CFP-Belgium Charter (Download here).
CFP-Belgium is member of the European CFP association (Commercial Film Producers of Europe – www.cfp-e.com), which currently unifies 22 European countries.
Agency: Darwin BBDO
Director: Abbie Stephens
Executive producer : Filip Vangeffelen
Producer : Femke Verstuyft
DoP : Sander Vandenbroucke
Foodshots : Nosh
Nosh Producer : Mady Angelino
Food DoP : Justin Bodenkamp
Creative director : Klaartje Galle
Creative team : Mathijs Bettens & Bjorn Blockx
TV Producer : Anna Vanheukelom
Account Director : Hannelore Van Cauwenberghe
Accounts : Sandra Demblon & Taraneh Atai Kachoui
Music : Gregory Caron


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Modified: February 28, 2017 3:35pm
The Subjects Caused Significant damage to an ATM Vestibule
Buffalo, NY – Erie County Sheriff Timothy B. Howard, Sheriff’s Detectives, and the Village of Akron Police Department are asking for the public’s assistance to identify two individuals associated with a felony criminal mischief incident at a Bank of Akron ATM.
The Village of Akron Police contacted the Sheriff’s Detective Bureau following an incident on February 25 where two males entered an ATM vestibule and caused a significant amount of damage within the space but did not damage the machine.
If anyone can identify the individuals in the security footage, they are urged to contact the Erie County Sheriff’s Office at 716.858.2903


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Best eggs ever and personal service!
By: Marsha Uselton (Apr 4, 2011)
Just a quick note to say I've been a member for several years and have enjoyed having fresh veggies delivered close to my neighborhood every Wednesday afternoon by truly caring folks! I love the early spring greens, as well as the herbs and produce through the summer. It's all pesticide free, and grown by folks commited to local agriculture. The eggs are noticeably fresh, sitting up with dark orange yolks. The spinach is clean and fresh. The sweet potatoes, corn, and beets are favorites as well.
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domain: food


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Buzznet Exclusive: AJR Premiere NYC Subway Performance
Hey Music pals,
You may remember me posting recently about the talented group called AJR! Well, they were chosen as Myspace’s “One To Watch” this month! In honor of that we have a video of them performing on a NYC Subway that you can get your eyes on before anyone else!! Enjoy!
As you saw, the boys talk about what it’s like to grow up in NYC and being in a band with family!
Follow @AJRBrothers and let them know you loved the video!
What are YOUR thoughts on family being in the same band??

domain: artistic


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Автор: Tony Plummer
Название:Forecasting Financial Markets: The Psychology of Successful Investing
Кол-во страниц: 400
Размер: 11 MB
Forecasting Financial Markets provides a compelling insight into the psychology of trading behavior and shows how "following the herd" can have disastrous results. It demonstrates how one's ability to make money in the world's financial markets depends critically on an ability to make decisions independently of the crowd. Forecasting Financial Markets details the three dimensions essential to achieve successful trading, including an ability to understand the forces at work in logical terms, recognize (and neutralize) any emotional responses to market fluctuations, and design an investment process or trading system that generates objective "buy" or "sell" signals. Taking the author's latest research into account, this book provides an in-depth assessment of the phenomenon of cycles, patterns of economic and financial activity, and how to use cycles as a forecasting tool.
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domain: psychology


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13 January 2018
Ghana Cocoa Board begins probe into utilisation of $1.8 billion loan
Chief Executive Officer of Ghana Cocoa Board (Cocobod), Mr Joseph Boahen Aidoo, says the government is investigating how an amount of $1.8 billion dollars secured for the 2016/17 cocoa purchases was utilised.
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domain: finance


Mark Galeotti: “The Vory: Russia’s Super Mafia” – Pushkin House Book Prize Finalist
(Moscow Times – themoscowtimes.com – Emily Couch – May 4, 2019 – themoscowtimes.com/2019/05/05/mark-galleotti-the-vory-russias-super-mafia-a65488)
In “The Vory: Russia’s Super Mafia,” Mark Galeotti draws on years of research to tell the grimly absorbing tale of Russia’s criminal underworld. Galeotti is a specialist in Russian history, security and crime who is a senior researcher at the Institute of International Relations (Prague); a regular contributor to international news outlets, and advisor to government and law enforcement agencies. He has been studying and writing about security issues and the Russian criminal classes since the 1980s. “The Vory” is both the culmination of decades of study and a primer for specialists and the general reader that charts the rise, fall, and reinvention of the criminal world from imperial Russia up to the present day.
Russian gangsters, Galeotti writes, “hold up a dark mirror to Russian society” in which the boundaries between crime, business and politics are “all too often indistinct.” The book’s central contention is that these gangsters “have not only been shaped by a changing Russia, they have also shaped it.”
Since the end of the 1990s, Russian organised crime has become “regularised, corporately minded and integrated with elements of the state.” If you follow Russian politics, chances are that you have seen the now famous clip of President Putin threatening to “‘whack terrorists, even in the outhouse.” That Putin publicly deployed these terms, Galeotti says, is only possible because of the widescale appropriation of the underworld lexicon by mainstream society. Krysha (roof, i.e., protection), skhodki (meetings), and limonki (little lemons, i.e., one million rubles) are just a few examples of underworld vocabulary – and, indeed, practices – that have become part of everyday political parlance in Russia.
Another central premise of the book is that where there is demand, organised crime supplies. In most cases the demand is for illegal and often violent services, but Galeotti also tells the lighter tale of “cheese runners” who smuggle Western cheese – forbidden by Russian counter-sanctions – into the country through Belarus. If 20 years ago organized crime was “a facilitator within Russia’s still unruly business environment,” today it plays a similar role on a transnational scale.
[Kindle:]Another “brand” in the world of vory are Chechen gangs. Part Three describes how Chechen culture and values have shaped this part of the underworld, garnering a reputation for ferocity and a particular kind of honor. Such is the strength of this image, Galeotti demonstrates, that Russian gangs often claim affiliation with Chechen groups to boost their own credibility.
The one demographic group that is largely marginalized in the Soviet and post-Soviet criminal world is absent from the ranks of vory is women. A female underworld of sorts did emerge in Stalin’s Gulags, Galeotti writes, but it was largely “shaped by its masculine counterpart” and denied official status by men. The elaborate and symbolic tattoos boasted by criminals frequently feature women but only as virgins or whores – the only two roles available to them.
[Paperback:]Given the multitude of mafia characters in popular culture, it is easy to fall into the trap of glamorizing their exploits and lifestyle. Galeotti, however, treads the line between demonization and vindication. Indeed, “The Vory” disabuses readers of the notion that organised crime consists of Marlon Brando style Godfathers presiding over defined, hierarchized clans. Instead, we are left with a much more unsettling picture of fluid networks with ever-shifting nodes of control. And although people in the West may fear this “global bug bear,” Galeotti reminds readers in the final chapter that it is the Russian people who are “the first and worst victim” of the criminal world of vory.
“The Vory” provides an exhaustively research and comprehensive yet accessible account of the rise and integration of an enormous and fierce criminal class in Russia. If you want to understand contemporary Russian society and politics, this book is essential reading.


While the whole world is waiting for the announcement of the mobile versions of the Ampere line of video cards, Chinese insiders continue to amaze.
We are talking about a gaming laptop fromAsus dubbed the G15 ROG Zephyrus GA503QS, VideoCardz reports. The device is being sold with a Ryzen 7 5800HS processor as the base and directly a portable version of the NVIDIA RTX 3080. The Ampere laptop line is expected to be announced at CES 2021, ie. in the very near future.


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The prestigious Library Journal has published a starred review of A Scourge of Vipers, the latest in my series of crime novels featuring Liam Mulligan, an investigative reporter for a dying Providence, R.I,. newspaper.
The review says: “Rhode Island Governor Fiona McNerney proposes the legalization of sports betting to reduce the state’s budget deficit. The mob opposes the idea because it would eat into its bookmaking business, and sports oversight groups claim it would open up games to dishonesty. After Atlantic City mobsters show up in Providence with bags of cash, presumably to influence legislators, veteran newspaper reporter Liam Mulligan investigates. When a state legislator and several other people turn up dead, Mulligan soon becomes a prime suspect in several murders. VERDICT DeSilva’s Edgar and Macavity Award-winning books (most recently Providence Rag) are a consistently well-written hard-boiled series. Few of the regular characters have roles here. Still, this excellent addition features a bit of romance, a lot of action, plenty of snappy repartee, and social commentary on the fate of newspaper journalism and the corrupting role of money in the political process. QUALITY ALL THE WAY.”
The book will be published on April 7, but can be ordered in advance here.


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Woman drives SUV into Waltham CVS; cashier injured
WALTHAM – A cashier was injured this morning when a Toyota Highlander being driven by a 72-year-old Weston woman crashed into four other cars and then into a CVS at the corner of Main and Harvard streets, police said.
The 43-year-old female cashier and the driver, neither of whom have been identified, were taken to an area hospital with minor injuries. Both had back, neck and shoulder pain, said Waltham Police Sgt. Brian Lambert.
The driver of the Toyota was attempting to exit the CVS parking lot when she hit four parked cars in the lot along Main Street.
The SUV veered into the north side of the CVS, facing Main Street, gouging the brick wall and breaking several windows, said Lambert.
The cashier was at the counter on the other side of the wall when the Toyota hit it, he said.
The Building Department told police the building is structurally sound, said Lambert, but damaged, and the area behind the counter is “unusable.” The drive-thru pharmacy was open Tuesday afternoon, but the rest of the store was closed, a store manager said.
The Toyota and two other cars were towed from the lot, he said.
A witness at the scene said the car was traveling “very fast.”Jen Judson can be reached at 781-398-8004 or firstname.lastname@example.org.