Bush gives timber industry CHRISTMAS-IN-JULY present
Worn out from its steady attack on the Chattahoochee National Forest, the Bush administration has asked Gov. Sonny Perdue to take over for a while. And, once again, the targets are some of the few remaining untouched parts of the 749,000-acre North Georgia forest.
In Georgia, about 64,800 acres, including Kelly Ridge and Mountaintown, have been deemed so ecologically sensitive that roads are forbidden within them. But that could change, thanks to a proposed rule announced July 12 by U.S. Secretary of Agriculture Ann Veneman.
The proposal would modify what's called the Roadless Rule. Under the change, the road protections would no longer be automatic. Instead, governors would have to request the stronger protection. That means Perdue would be allowed to decide whether loggers and four-wheel drive enthusiasts could have access to these sections of the forest.
Nationwide, 58.5 million acres will be affected.
So far, the governor's office is quiet. Perdue's spokesman says the governor is taking his time and consulting with the Department of Natural Resources. His decision is anxiously awaited by environmentalists, timber company executives, and ATV drivers, all of whom are crossing their fingers that the governor comes down on their side.
"Hunters, hikers, boaters, anglers and families all seek the recreation and solitude that places like Kelly Ridge, Mountaintown and other public lands provide," says the Sierra Club's Kate Smolski. "All Americans, not solely the timber industry, should be able to enjoy these special places."
This is the latest blow to the North Georgia forest. In January, the U.S. Forest Service refused to extend its highest level of protections to two areas of the Chattahoochee — Kelly Ridge and Mountaintown — despite a massive outpouring of public comment in favor of the protections. At the same time, the Forest Service declared 461,000 acres "suitable" for logging and off-road vehicles.
For what it's worth, the Forest Service is accepting public comment on the change to the Roadless Rule until late September. E-mail comments to email@example.com.
-- Michael Wall


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© Foto: Heinz Heinkel
A fresh face
02. Nov 2021
Sarah Weiss is the new professor of ethnomusicology at the University of Music and Performing Arts Graz. In our podcast, she talks about what is important to her in teaching and what interests her most in this particular field.
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domain: artistic


I agree totally that the bacteria mass will self-regulate. That wasn't what I meant. My point is the bio-wheels, which work by providing a more aerobic/oxygen-rich environment for the bacteria (allowing greater bacterial activity), are very
Here is the issue with a planted tank as I understand it: if you have an excess of very effective biological media substrate of any type (providing lots of oxygen to the bacteria, allowing to work at their most optimum) the bacteria can metabolize fish waste faster than the plants can take it up. Fish's "poop" and urine (nitrogenous waste products/ammonia) are plant food
. This can lead to less than optimal plant growth. In addition, the more water agitation you have, the more CO2 is driven off from the system. That's another reason to not use the bio-wheels.
Please feel free to check other threads in this forum or on the planted tank forum for corroboration. Planted tanks are handled a little differently. You want minimal surface disruption and modest, not maximum, bio-filtration. Plants do help the water quality issue by uptake and conversion of waste products, but the bacteria have the potential to out-compete the plants for certain nutrients.


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With the hectic weekend behind, it was time to put the hiking boots back on to test the comfort level not just the damaged foot, but myself in general. Today’s urban hike is the Mission Trails Regional Park in Santee, the largest open space preserve in California. The East Fortuna entrance is immediately south of 52 at the Mast exit.
For a decent hike East Fortuna offers access to some good trails with a bit of variety, Mission Dam, Oak Canyon, but not easily to the Visitor's Center. For a mountain bike workout try any of the entrances in Tierrasanta such as the end of Clairmont Mesa Blvd, which has lots of parking and restrooms. The mountain bikers should call “On Your Left” as they approach hikers from behind – that’s the hikers queue to step to the right and let them pass. If you’re on the bike please shout it out, us hikers expect it and appreciate it!
The Visitor Center is located in more of a rock climbing area. While walking toward Mission Dam in about 3/4 mile there is an old metal foot bridge over the San Diego River providing access to the trails.
East Fortuna has a staging area for equestrians, it has ample parking, utilitarian restrooms and a doggie water fountain. Behaved, leashed, dogs are welcome on all the trails. At all the trailheads and locations throughout the park are trash and recycle containers. Despite that I did see a couple beer cans, that’s just mean! They’ve now been recycled.
From the trailhead it’s a short half mile to where the various loops and crossings begin. All trails are well marked, it would be very hard to get lost. If in doubt listen for the traffic on 52 and follow the traffic back to the parking area. Really, Mast south only goes to the parking area.
Grasslands Crossing is a ‘T’ intersection with the trail left and right. Note the sign only shows it going left, GO LEFT!
GO LEFT, the shade under the 52 overpass is tempting, but go left - here’s why. Go right and you’ll reach the Park Boundary in a few yards. No other signs and there’s a wide service road onto the Marine property of Camp Elliott. Best known as ordnance training area during WWII, it was also home to original 29 Navajo Code Talkers. The Marine ATV patrols will turn you back, detain you, or may even confiscate your mountain bike. Go LEFT! (OK I went right, but just to take the picture)
From the Grasslands Crossing Trail are loops to take as options. This need not be an out and back hike.
There are informative signs along the trails, and evidence of the early Kumeyaay inhabitants. It could not have been easy to create moteros in the conglomerate rock.
It does not take long to reach Mission Dam. A bit of a marvel in its day to harness the San Diego River to supply Mission de Acala with reliable water.
Yes there is a campground in Mission Trails! Friday/Saturday only, but today I want to retrace a hike I did with my daughter some time ago, following Oak Creek back up thru the canyon.
The Oak Creek Trail! I did this with my daughter so long ago, back when California had water! It was bliss then, hiking along a babbling creek, water falls, pools, wildlife. It was green! Now it’s brown (aka California’s Gold) and the only wildlife is a bee hive in the tree.
There are a series of ‘Time Line’ signs along the trail, providing the history of the area. But I’d rather had water in Oak Creek!
I’ve reached the return point on this adventure. About 5 miles, but the foot feels good. The East Fortuna staging area is less than a mile over the hill.
I did see a few late blooming wildflowers. Enjoy!

domain: geography


Need a robot with a soft touch? A team of Michigan State University engineers has designed and developed a novel humanoid hand that may be able to help. In industrial settings, robots often are used for tasks that require repetitive grasping and manipulation of objects. The end of a robot where a human hand would be found is known as an end effector or gripper.
The novel humanoid hand design is a soft-hard hybrid flexible gripper. It can generate larger grasping force than a traditional pure soft hand, and simultaneously be more stable for accurate manipulation than other counterparts used for heavier objects.
The team explained that the design novelty resulted in a prototype demonstrating the merits of a responsive, fast, lightweight gripper capable of handling a multitude of tasks that traditionally required different types of gripping systems.


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New home cleaning services in Dover for Low Price.
If you want to clean your home and you’ve been wondering which house cleaning Services. Company will be best for you, then you should look no further. Ocean Blue Services is a professional cleaning outfit that offers some of the best cleaning services you will ever find in Dover and environs. We are very reliable as our home cleaning services are top notch and we ensure that your house is properly cleaned up and as soon as possible. Our services are among the best in Dover due to the following reasons:
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Teamwork: We work as a team and you will have a team of at least 3 people coming around to clean your home. With this, your home should be cleaned within a shorter time, compared to if it was only one person cleaning it. Furthermore, we try to send the same teamas much as possible on cleaning your home every time. This is considering that they will already be familiar with your house and your preferences.
House Cleaning service Dover in best price.
The house cleaning professionals of Ocean Blue Services love to serve the folks in Dover, Massachusetts. Dover is a great place to visit and a great place to stay. This small New England town in Norfolk County is right outside of Boston, only 40 minutes from the big city’s downtown area. Close enough to Boston for easy access, but far enough for visitors’ and residents’ enjoyment of a small town’s quiet and rural atmosphere, Dover is an idyllic spot in beautiful eastern Massachusetts.
Dover has more than 350 years of recorded history, and has gone through a name change in its three and a half centuries. Originally called “Springfield” during the era when it was unincorporated, in 1836 it was finally made into an incorporated town and named “Dover,” after the original home of the chairman of the town’s parish committee, an emigrant from England.
Steeped in history as it is, the town has many residents who keep the past alive in the country’s memory. Taking great care to preserve Dover’s history, for almost a century the town’s historical society has maintained and preserved a Revolutionary War-era home, the Benjamin Caryl House, which it operates today as a museum.
Dover has a rather unusual style of government that dates back to its incorporation, the Town Meeting form of government, which was granted to it by a charter. The town is governed by an elected board of selectmen, and does not have a mayor like many other U.S. towns and cities. The selectmen and voters of Dover officially meet in the town once a year for discussion, elections, and decision-making. Sometimes additional town meetings are called for special reasons.
The climate is similar to Boston’s, Dover being near the coast. The Dover, MA area is warm in the summer, temperatures reaching upwards of 80 degrees Fahrenheit at times, and generally staying above 32 degrees Fahrenheit in the winter. Condo and residential cleaning is a service Ocean Blue Services team provides in Dover year-round, making it easy for residents to enjoy the flow and life of all the seasons there.
A gorgeous area with deep agricultural roots, Dover has a rural feel to this day. Visitors and residents can still see the farmhouses, barns, and stone walls of yesteryear. The Charles River runs through Norfolk County and provides much beauty for Dover’s citizens.
Recreational opportunities in the Dover area abound. Aficionados of many forms of outdoor recreation will find satisfaction here: there are places to go boating, horseback riding, and fishing. For those enjoying walking, jogging, and running, the area features about 2000 acres with hundreds of miles of walking trails. For those with active outdoor lifestyles, residential cleaning will be especially necessary—live life outside, and leave the cleaning to the professionals of Ocean Blue Services!
Since the residents of Dover have such abundant opportunities to enjoy the outdoors, it is no surprise that many Olympians and athletes hail from the town, including two medal-winning equestrians. Dover has also been home to government officials serving the country in various capacities, as well as a couple authors and two actresses.
Dover, MA has seen more than 350 years of settlement and dozens of generations growing up in its picturesque setting. If you live in the Dover area and are looking for the very best in residential cleaning, please contact the professionals of Ocean Blue Services. You will love our staff as much as we love Dover!
If you want a professional and smooth house cleaning Dover, then you can rely on Ocean Blue Services.Our highly trained and motivated staffs will make sure you get a high-quality apartment cleaning service every time.
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Fair trade fan ‘a superhero’
Primary school teacher Keren Rego is the winner of the inaugural Fairtrade Supporter Award.
The awards were set up by Fairtrade Australia New Zealand to acknowledge individuals who champion the concept within their communities.
Rego has been educating her students and other staff at Pt Chevalier School about the benefits of buying fair trade products.
She has set up a fair trade class at the school and organised numerous events including a street march to a nearby supermarket.
‘‘When it comes to social justice, she is a superhero,’’ awards judge Rachael Le Mesurier says.
‘‘With never-ending enthusiasm, she has taught and inspired generations of kids about fair trade, sustainability and how to make the world a better place.’’
Rego was presented with her surprise award at a school assembly on August 15.
‘‘I am very passionate about fair trade and not a week goes by without a child in my own year 1 class or another class rushing up to me in the playground with a fair trade sticker or picture for me, which is a lovely reminder that my enthusiasm is paying off,’’ she says.
Rego will also have the opportunity to travel to a fair trade cooperative in Costa Rica next year.


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MIPIM 2016: This government will deliver 1m homes by 2020 – including 200,000 starter homes. To do that, we need developers and major housebuilders to continue to build out; indeed, we want them to expand and build more. We will help, with direct commissions on public sector land.
What any developer requires – whether private or public sector – is confidence and continued support from their financial investors. And those investors need certainty over wider market conditions.
Brexit would inevitably cause investor uncertainty, which will at least slow, if not stall, the investment we need to keep building the houses we need. Without overseas investment, remember, many of our key developments – especially in London – just won’t happen. And that includes those plans for affordable homes.
For that reason, notwithstanding my feelings about the institutions of the European Union (a view I share with the prime minister), I support David Cameron’s reforms and his campaign for Britain to remain in a reformed EU. Whatever changes we think are required to benefit any industry, including housebuilding, it is important for the British government to be at the negotiating table.
The worry about possible Brexit loomed large at MIPIM in Cannes last week. The property industry, looking to invest, and British towns and cities, promoting their land to international investors – all were concerned about the impact of the UK leaving the EU.
Our property industry is international. Without access to that wide range of finance it would be crippled.
But the concerns are not only financial. Of course, as a government we are working hard to fill the skills gap in the construction industry, with a massive expansion of the apprenticeship programme. Meanwhile, we still need access to the skilled labour market that EU membership makes available to us.
Leaving would also have an impact on our supply-line export companies: 3m people are involved in such activities. And it could disrupt the imports of the supplies needed for building, from bricks to modular build models, delaying the development of the advanced construction techniques which will increase the speed of build out.
Outside the EU, we will still have our current planning system, with all the protections in place from Natural England and other agencies and councils.
We are already building a system where every council works proactively with communities and developers to identify the best land for housing – and that is how we increase housing, not by creating years of uncertainty.
To continue towards our target of 1m new homes – and meet that commitment on affordable housing – I am convinced that the UK cannot afford to leave the European Union. After MIPIM I know now that the vast majority of our housebuilding industry – and the investors who underpin it – agree with me.

domain: economics


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Evelyn Sharma known for films like Yeh Jawaani Hai Deewani and Saaho, got married to Australia-based dental surgeon and entrepreneur, Dr Tushaan Bhindi in Brisbane on May 15 this year. The actress kept the news of her wedding under wraps until she recently shared a picture from her nuptials on social media.
Dressed in a white bridal gown, Evelyn is seen sharing an eyelock with her groom Tushaan who looks dapper in a navy blue suit. The Yaariyaan actress captioned the picture as, "Forever."
Have a look at some more pictures from Evelyn and Tushaan's wedding.
Intially, the couple had planned to have a grand wedding in India, but had to postpone their plans owing to the COVID-19 pandemic.
As per a report in Hindustan Times, while speaking about her country wedding, Evelyn told a leading daily, "There's no better feeling than being married to your best friend. We're so excited about spending our life together. We started our wedding celebrations with a legal ceremony. Of course, we would have loved for our family and friends from around the world to be present, but we know their blessings are always with us."
Reportedly, the newlyweds now plan to follow up their intimate wedding with a grand reception which will be hosted on a later date.
Evelyn and Tushaan met in 2018 on a blind date set up by one of their friends. In 2019, Tushaan went down on his knees on the famous Sydney Harbour Bridge and popped the question to Evelyn. He got a guitarist to play their favourite songs and read out a special note penned by him.
Later, the actress had opened up on Tushaan's proposal while speaking with ETimes and said, "It was a dream come true! Tushaan knows me so well... his proposal was perfect!" In the same interview, Evelyn had also said that she would love to shift to Australia post marriage as it's one of her favourite cities in the world.
Workwise, Evelyn Sharma was last seen in Prabhas-Shraddha Kapoor's Saaho.

domain: entertainment


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Peacehaven – Ascension
Development along the cliffs between Brighton and Newhaven started in the early C20 and intensified after World War I. The area east of Telscombe Cliffs, which was actually in the parish of Piddinghoe from which it was separated by the Downs, was initially called New Anzac on Sea in honour of the Australian and New Zealand Expeditionary Force in World War I, but it soon became known as Peacehaven. Initially, most houses built on the numerous small plots were bungalows, but more substantial ones appeared later.
A temporary church was opened in 1929 (VCH 7 p66). Efforts to build a permanent one started before World War II, but by 1950 had met with no success. A design combining church and hall by a local man who was not an architect, W A Crossley, aroused the disapproval of Bishop Bell of Chichester. In his unpublished memoirs, J L Denman (BAL/MSS DeJ/7/4) describes being summoned by the Bishop after he had advised against this design. He also advised against one by G Haynes of East Blatchington, on the grounds that he was a local government employee. Denman himself prepared a design (ibid), but in the event, the church was entrusted to the safe though unadventurous hands of L K Hett and built in 1954-55 (TCA 3 p115) at a cost of £15,000. On completion, Peacehaven became a full parish.
Hett’s church is built of brick, with a west tower which is combined with the main entrance. This is beneath a round-headed west window, the only one on the church of this form. The long aisle windows are square-headed and the short sanctuary that projects to the east has no east window. Inside, the proportions of the nave are broad and low and its roof is the dominant feature with its prominent canted trusses. Both these are characteristic devices used by Hett and the work here recalls in particular his rather earlier St Richard, Haywards Heath, West Sussex. A north chapel was added at the east end of the nave in 1985 (church website).
Font: Octagonal with a single quatrefoil on each side and a slender stem. It is not older than the C19, but does clearly predate the church. Its previous history is not known.
My thanks to Nick Wiseman for the photographs


I’ve reported on the opening of the Lakeview Care Home in Lightwater, firstly with a time lapse slide show of the construction, and secondly on the interior and the opening event. 28 more words
A slide show of the construction of the Lakeview Care Home in Lightwater was the subject of an earlier article. In this photo blog post there are pictures of the interior of the care home, along with some of the grand opening on the Thursday, 6th November.
Everyone loves a bit of Queen right? In tribute to their musical greatness I thought I’d highlight a few moments they’ve contributed to cinema.
Whether – like the first two in the list below – they were responsible for almost the film’s entire soundtrack, or one of their songs were used in a particular scene, a bit of Queen goes a long way. 220 more words
As a big fan of Les Mis, I always love seeing a new interpretation of the storyline, so needless to say, when I heard there was a radio theater production, I had to check it out. 2,138 more words
Some of you might remember a BBC gritty television police drama series of the 1960’s and 70’s called Z Cars.
I was reminded of the series while listening to a wonderfully entertaining and uplifting talk by Brian Blessed yesterday, as he gave the address at the grand opening of Lakeview Care Home in Lightwater. 147 more words
Whether you call it No Shave November or Movember, it’s the time of year that has been dedicated to bringing male health concerns to the fore. 828 more words


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H.R.6278 - AVERT Future Gun Violence Act115th Congress (2017-2018)
|Sponsor:||Rep. Clark, Katherine M. [D-MA-5] (Introduced 06/28/2018)|
|Committees:||House - Judiciary|
|Latest Action:||House - 08/06/2018 Referred to the Subcommittee on Crime, Terrorism, Homeland Security, and Investigations. (All Actions)|
This bill has the status Introduced
Here are the steps for Status of Legislation:
Summary: H.R.6278 — 115th Congress (2017-2018)All Information (Except Text)
Introduced in House (06/28/2018)
Animal Violence Exposes Real Threat of Future Gun Violence Act of 2018 or the AVERT Future Gun Violence Act of 2018
The bill expands the categories of persons who are prohibited from receiving or possessing a firearm.
Specifically, it prohibits firearm sale or transfer to and receipt or possession by an individual who has been convicted in any court of a misdemeanor crime of animal cruelty.

domain: politics


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Select a stripe or border print fabric. Use the Creative Grids 60 degree Triangle Ruler, add borders and a simple faux binding with the Dual Angle Quick Easy Mitered Binding Tool by Sew Biz to create this unique table topper.
On “A Wing and a Prayer” and a “shoestring”, I opened the doors of SEW BIZ on June 20, 1983.
After 35 years, I have CLOSED the Retail Store. We are now continuing to add items to our Online Store and we will be keeping you informed of NEWS regarding sewing and quilting through our FaceBook Page and our Email Blasts.


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A month may not seem like a long time in the grand scheme of things, but it is an important milestone in a relationship. If you and your partner have been together for four weeks and the following things are happening, it could be a good sign for the future of your relationship:
1. You've spent time with him in the daylight hours
While it may seem humorous, many modern relationships begin as casual hookups. While there is nothing inherently wrong with this, it's important to recognize that some relationships may be purely sexual. However, if a man is willing to spend time with you during the day and even initiates these hangouts, it could be a sign that he is interested in more than just a casual physical relationship. This may indicate a desire for a deeper connection and the possibility of a long-term relationship.
2. He responds to texts within a decent time frame
It's important to pay attention to how someone responds to your texts, as it can give you insight into their level of interest. If they are consistently quick to respond and put effort into their replies, it could be a good sign that they are interested. On the other hand, if they take a long time to respond or their replies are careless or sporadic, it may be a sign that they are not as invested in the conversation or relationship. It's important to consider this when deciding how much time and effort to put into communication.
3. He's eager to make plans and he actually keeps them
If you're not interested in a guy, you may feel comfortable flaking out on him because you don't feel as invested in the relationship. On the other hand, if you really like someone, the thought of canceling plans or not putting effort into the relationship likely doesn't occur to you. When a guy is consistently showing interest and making an effort to see you, it's a good sign that he values the relationship and is actively participating in it. You should look for a partner who is excited to spend time with you and doesn't expect you to always initiate plans or take the lead.
4. You're no longer afraid of opening up and being yourself
To avoid sabotaging your relationship, it's important to let go of your past experiences and not let them influence your present actions. If you feel comfortable being yourself with your partner without worrying about past mistakes, it's a good sign that your relationship is strong.
5. You've met the friends and even some family and vice versa
If you are genuinely interested in someone, you likely want to introduce them to your social circle and family. Doing so can help your relationship grow and give you a better understanding of how your partner interacts with the people you care about. It's important to get this out of the way early on so that you can see how your friends and family perceive your partner and vice versa.
6. You've had a fight and it was actually handled well
It's normal for couples to have disagreements from time to time. These disagreements can be an opportunity to see how well you and your partner handle conflicts and communicate with each other. If you are able to get through a disagreement without losing your temper or becoming overly emotional, it can be a sign that your relationship is worth pursuing.
7. He's shared one or more of his favorite things with you
If someone is truly interested in a long-term relationship, they will want to share their interests and passions with their partner. If your partner is not interested in introducing you to their favorite shows, music, or hobbies, it may be a sign that they are not looking for a long-term relationship. It is important to be open and willing to share your interests with your partner in order to build a strong foundation for a lasting relationship.
8. He genuinely respects you
Respect is an important part of any healthy relationship. If your partner does not show you respect, it may be a sign that the relationship is not worth pursuing. Look for signs of respect in small actions, such as the way your partner speaks to you in public or how they prioritize your needs. If you do not feel respected in your relationship, it may be best to move on to avoid being hurt.
9. Your intimacy levels match
Deciding whether or not to have sex in a new relationship is a personal choice and should be based on what feels right for you. If your partner is pressuring you to have sex before you are ready, it may be a red flag. It's important for both partners to be on the same page when it comes to sex and to be able to communicate openly about it. Good communication is key to a healthy and fulfilling relationship.

domain: sociology


THE funeral of a father and son who died in a suspected murder suicide has been told "there are no answers".
Tadg and Diarmuid O'Sullivan were laid to rest today, with grieving wife and mum Anne in attendance.
Tadg, 59, and Diarmuid, 23, are believed to have shot son and brother Mark a number of times in his room early on Monday — before turning the guns on themselves in a nearby Co Cork field.
Tragic 26-year-old Mark — who suffered up to seven wounds after his dad and brother shot him with their legally held .22 rifles — will be buried at an unnamed cemetery but not in the same plot as his dad and brother.
Gardai believe Monday's horror unfolded because of a dispute over a will being considered by Anne, the owner of the valuable 115-acre farm which has been in her family for many years.
She was planning to leave the property to eldest son Mark.
But Diarmuid was said to be deeply upset over this decision believing he should be left a share of the land — and his dad sided with him.
Legal documents found by cops after the shooting showed that the matter had developed into a contentious legal dispute between the divided family.
Mourners at St Mary's Church in Castlemagner for the funeral of Diarmuid and Tadg were told the triple tragedy had cast a dark cloud over the area - and the news of the loss of three lives was "incomprehensible".
In accordance with Covid-19 public health restrictions, the funeral for the two was held privately for family only.
Parish Priest Father Toby Bluitt told mourners: "Today’s Gospel from Luke which depicts the Passion of Christ speaks of a darkness coming over the whole world, indeed it seemed like that same darkness, the darkest hour of creation - enveloped the O’Sullivan family and the local community here in Castlemagner over the past number of days.
"The normally tranquil local area blanketed at this time of year with a myriad of colourful autumn leaves became a hive of activity and the Autumn light was, for a time, a very distant memory.
"The shock, the numbness, the devastation, was impossible to imagine and the unfolding news of the loss of three lives was incomprehensible.
"Just like all of you, I too am struggling to make sense of this life changing tragedy."
TRIBUTES TO PAIR
Fr Bluitt said Tadg was "accommodating, friendly and happy in his chosen field", having worked in the motor trade for over 40 years.
He said "one would imagine life was full of possibilities" for Diarmuid, who had been due to graduate in accounting from Cork Institute of Technology next week.
Fr Bluitt added: "One could also say that both Tadg and Diarmuid touched many people’s lives along the way as they journeyed through life.
"Their lives and deaths have changed you all and you will never be the same again.
Most read in News
"So today, gathered together in our grief, we do not minimise the loss of their lives by trying to provide easy answers.
"Because there are no answers."
Mark’s funeral will be held tomorrow at the Church of the Immaculate Conception in Kanturk. It will be streamed online at 3.30pm.


Agenda item - BH2020/01756 - The White House, Roedean Road, Brighton - Full Planning
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BH2020/01756 - The White House, Roedean Road, Brighton - Full Planning
RECOMMENDATION – GRANT
Ward Affected : Rottingdean Coastal
Demolition of existing dwelling house (C3) and erection of 3no three storey detached dwelling houses (C3) with associated landscaping, car and cycle parking, revised access and vehicle crossover.
(1) It was noted that an in-depth presentation had been provided by officers in advance of the meeting and was included on the council website detailing the scheme by reference to site plans, elevational drawings and photographs which also showed the proposed scheme in the context of neighbouring development.
(2) The application site comprised a two storey detached property in use as a single dwelling house on the northern side of Roedean Road. It was well set back within the site with a large front lawn which sloped down towards the long curved driveway. Access onto the road was shared with the Ocean Heights development to the east. The proposal would provide three new dwelling houses of a good standard of accommodation which were considered to be well designed and to contribute towards creating a sustainable neighbourhood with improved biodiversity and ecology without having an adverse impact on the national park, neighbouring amenity, highways or archaeological assets. Approval was therefore recommended.
(3) Councillor Mears spoke in her capacity as a Local Ward Councillor setting out her objections and those of local objectors. In her view the proposed development would be gross overdevelopment of the site, would be bulky, covering the width of the plot would cause overshadowing and would be too close to the boundary with Roedean Road. The level of on-site parking provision was considered to be too low and it was also considered that there would be a serious safety issue as a result of traffic using access to the site on a blind bend.
(4) Ms Sheath spoke on behalf of the applicants in support of their application explaining that this application had been put forward following lengthy pre and post application discussions with officers. The development would have no negative impact on neighbouring development or the National Park. Access arrangements had been assessed but were considered appropriate for the level of development proposed given that access issues referred to already existed.
Questions of Officers
(5) The Senior Planning Officer, Russell Brown, clarified the position in respect of hedges/screening on site.
(6) Councillor Janio referred to the location of trees including any which were protected on site and this was clarified.
(7) Councillor Theobald sought clarification of the size of the garden area for each dwelling unit and whether this site was under the control of the same developer as the neighbouring one. Confirmation of the amenity space provided was given and it was also confirmed that the two sites were in separate ownership.
(8) The Chair, Councillor Littman, referred to the access arrangements to the site, whether they were in the ownership of the applicants and whether it would be appropriate for further improvements to be required including provision of a footway. It was explained that the existing substantial verge formed part of the adopted highway and that to require further improvements in view of the size of the development was considered to be disproportionate.
Debate and Decision Making Process
(8) Councillor Theobald stated that in her view the proposed scheme represented overdevelopment of the site. The resulting dwellings would be bulky, covered the whole site and would be built right up to the boundary, she was unable to support it.
(9) Councillor Miller concurred agreeing that too much was proposed for the size of the site and would result in loss of amenity and overlooking.
(10) Councillor Childs stated that he was of the view that the three dwellings proposed were acceptable and would make a contribution to the city’s housing supply.
(11) Councillor Yates stated that on balance he considered the proposed scheme was acceptable and that he would be voting in support of it.
(12) A vote was taken and on a vote of 7 to 2 planning permission was granted.
59.3 RESOLVED – That the Committee has taken into consideration and agrees with the reasons for the recommendation set out in the report and resolves to GRANT planning permission subject to the Conditions and Informatives also set out in the report.
Note: Having declared a prejudicial interest in the above application Councillor Fishleigh withdrew from the meeting and took no part in the debate or decision making process.
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EDMONTON, Alberta (AP) - Lydia Ko is back on top of the Canadian Women's Open leaderboard.
The 16-year-old Ko, the winner last year at Vancouver Golf Club at an LPGA Tour-record age of 15 years, 4 months, shot a 5-under 65 on Thursday in perfect conditions at Royal Mayfair for a share of the first-round lead with Angela Stanford and the Netherland's Christel Boeljon.
Ko, the South Korean-born New Zealander, had six birdies and a bogey.
Stanford rebounded after going 0-4 last week in the United States' Solheim Cup loss in Colorado. The Texan birdied five of the first 12 holes in her bogey-free round.
Boeljon had a hole-in-one on the 16th hole. She also had a bogey-free round, playing Nos. 15-17 in 4 under.
Copyright 2013 Associated Press. All rights reserved. This material may not be published, broadcast, rewritten, or redistributed.


In an extremely rare sight, a wild gorilla has been captured on camera cuddling with her newborn baby. Moments like this are rarely noticed, let alone captured on tape. Yet, a group of conservationists managed to witnessed the lovely scenes in the Nouabalé-Ndoki National Park, in the Republic of the Congo.
While observing the western lowland gorilla population in the Nouabalé-Ndoki park, a research team from the Wildlife Conservation Society came upon a gorilla mom and her one-week-old baby. The two offered an emotional display of mother-baby bonding. Gorillas are usually overprotective when comes to their newborn babies and won’t let anyone getting close to them in the first weeks after getting birth.
In their weeks of like, the baby gorillas rely on their mom 100% for food, body temperatures and of course protection. Even so, with all the efforts, baby gorillas still have to face some extremely tough challenges as only one in three newborns get to the adulthood. Unfortunately, the odds are cutting lower due to human hand, too.
You can watch the beautiful moment, here:
“Many of the factors that count against baby gorillas are natural hazards linked to their environment, such as predators – mostly leopards,” Wildlife Conservation Society Congo director, Mark Gately told National Geographic. “Unfortunately there is also a significant risk posed by human activities.”
Western lowland gorillas are part of the critically endangered species list, after their numbers dramatically decreased over the last decades. Right now, there are around 150,000 to 250,000 western lowland gorillas in the wild and nearly 60% of them live in the Republic of the Congo.


I am a lifelong resident, homeowner and constituent in Burbank. The conduct of Mayor Konstantine Anthony has been a terrible reflection on the city of Burbank and its residents.
Specifically, the political event in Santa Clarita where he was slapped on his backside, which was broadcast several media outlets and social media.
Additionally, his book on abolishing police is openly [displayed] on his bookshelf in his office. As a law enforcement professional, I can’t even imagine what your police think of that glaring at them when they have to talk to him. He is telling them that their profession doesn’t matter and they are not wanted. Isn’t that some sort of violation of city policy regarding bias?
As a constituent, I am requesting you immediately remove him as mayor and conduct an investigation into his inappropriate behavior. Ultimately, he should be removed from Burbank City Council. He definitely does not represent our fine city.
First published in the September 16 print issue of the Burbank Leader.


Welcome to SB Nation Reacts, a survey of fans across the NFL. Throughout the year we ask questions of the most plugged-in Los Angeles Rams fans and fans across the country. Sign up here to participate in the weekly emailed surveys.
How confident in the direction of the team is the Los Angeles Rams fan base after a Week 3 loss to the Cincinnati Bengals, where the Rams defense played relatively well but the offense could not get much going? Are fans still confident in the offensive play calling? With Alaric Jackson potentially out, are fans trusting that the coaching staff can make adjustments after giving up six sacks? Do they trust that Raheem Morris can get more edge contain with the current defensive players on the roster?
These are all questions to consider when thinking about the direction of the future in Los Angeles. The Rams are only 1-point underdogs currently per DraftKings Sportsbook, heading to Indianapolis for a Week 4 matchup with the Colts.
Make your choice in this week’s SB Nation Rams Reacts survey!


text:
If you didn’t have the birth you’d hoped for…
If you worry about how a “negative” birth experience has imprinted your child,
If you didn’t know about Ecstatic Birth when you were having kids, but now you do and feel any twinge of regret, you will want to watch this!
Fall… back to school.. re-entry into our new masked world…
This time of year always feels like a new beginning, but now more so than ever.
Fresh starts are a period of conscious creation.
A time to feel into and honor our DESIRE.
What is true for you now?
Where do you want to go?
Our brains are whirring with thoughts of the future.. what is going to happen?
With the pandemic? With the election? With schools? With racism? With hospitals?
With birth… with_________???
Couldn’t we all use some nourishment and healing at this moment in time?
When so much is at stake it can feel counterproductive to take pause and drop in, but that is precisely the medicine of this time.
Rituals can offer us a potent portal as we navigate the waves of our life.
Rituals help us access our somatic and primal brains and with it our deeper truths and knowings.
There is something that deeply troubles me about our legacy….
That moment when a baby is born and a mother emerges is magical, sacred.. POWERFUL.. The highest of highs. Right?
On a personal level, for both parents, it is one of the major milestones of life. For the baby, it is literally LIFE itself.
On a global level, this moment ushers in the next generation of humanity.
It is DIVINE- the moment creation is actualized.
The FUTURE is literally being BORN.
A pivotal EXTRAordinary moment on the planet…
and yet our legacy declares that THIS is the moment that we will PUNISH WOMEN FOR THEIR SINS AGAINST MAN.
As birthworkers we know deep in our souls that birth is a sacred and profound rite of passage in a woman’s life complete with the potential for joy, empowerment, transformation and ECSTASY.
However, the reality of what we witness every day from inside and outside the birthing rooms is quite different.
It can be exhausting to hold the vision for the highest version of birth as you know it and support women in a climate that often feels like a direct contrast.
If you are a birthworker passionate to elevate the experience of birth for your clients and the world at large, I’d like to invite you to a unique and complementary online training to support you in your mission:
Pain to PLEASURE: Supporting Women in Ecstatic Birth

domain: literature


text:
Brassica juncea, a worldwide cultivated crop plant, produces seeds of different colors. Seed pigmentation is due to the deposition in endothelial cells of proanthocyanidins (PAs), end products from a branch of flavonoid biosynthetic pathway. To elucidate the gene regulatory network of seed pigmentation in B. juncea, transcriptomes in seed coat of a yellow-seeded inbred line and its brown-seeded near- isogenic line were sequenced using the next-generation sequencing platform Illumina/Solexa and de novo assembled. Over 116 million high-quality reads were assembled into 69,605 unigenes, of which about 71.5% (49,758 unigenes) were aligned to Nr protein database with a cut-off E-value of 10−5. RPKM analysis showed that the brown-seeded testa up-regulated 802 unigenes and down-regulated 502 unigenes as compared to the yellow-seeded one. Biological pathway analysis revealed the involvement of forty six unigenes in flavonoid biosynthesis. The unigenes encoding dihydroflavonol reductase (DFR), leucoantho-cyanidin dioxygenase (LDOX) and anthocyanidin reductase (ANR) for late flavonoid biosynthesis were not expressed at all or at a very low level in the yellow-seeded testa, which implied that these genes for PAs biosynthesis be associated with seed color of B. juncea, as confirmed by qRT-PCR analysis of these genes. To our knowledge, it is the first time to sequence the transcriptome of seed coat in Brassica juncea. The unigene sequences obtained in this study will not only lay the foundations for insight into the molecular mechanisms underlying seed pigmentation in B.juncea, but also provide the basis for further genomics research on this species or its allies.
Citation: Liu X, Lu Y, Yuan Y, Liu S, Guan C, Chen S, et al. (2013) De Novo Transcriptome of Brassica juncea Seed Coat and Identification of Genes for the Biosynthesis of Flavonoids. PLoS ONE 8(8): e71110. https://doi.org/10.1371/journal.pone.0071110
Editor: Tianzhen Zhang, Nanjing Agricultural University, China
Received: April 8, 2013; Accepted: June 25, 2013; Published: August 19, 2013
Copyright: © 2013 Liu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by the National Natural Science Foundation of China (No. 31101176, No. 30971799 and No. 31271762). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Brassica juncea (L.) Czern & Coss is grown in China, India, Canada, Australia and Russia as an oilseed, condiment or vegetable crop , , . This species produces black, brown or yellow seed. The varieties distinct in seed color have many differences in agronomic traits such as oil and protein content , , seed dormancy , seed germination . The seed pigments of the Brassica species are predominantly secondary flavonoid metabolites, i.e., flavonols and proanthocyanidins (PAs) , . Flavonols accumulate in cotyledons and give seeds a yellow cue while PAs, also known as condensed tannins, deposit only in seed coat and bring about browning through oxidation . PAs, oligomers of (−)-epicatechin, are synthesized in the late stage of flavonoid biosynthesis from dihydroflavonols by catalysis of dihydroflavonol 4-reductase (DFR), leucoanthocyanidin reductase (LODX) and anthocyanidin reductase (ANR) . The genes encoding these enzymes and many other genes participating in flavonoid biosynthesis have been cloned from a dozen of plant species including Arabidopsis , grape , soybean . In Arabidopsis, mutants of the genes for flavonoid biosynthesis produce transparent testa (tt) and yellow or pale yellow seed. However, only few genes regulating flavonoid biosynthesis have been found in Brassica species, close allies of Arabidopsis. For example, in B. napus, DFR is found to be associated with seed pigmentation by comparison of DFR activity and PA accumulation in seed coat between a yellow-seeded line and its related black-seeded counterpart . In B. juncea, DFR and LODX are confirmed to participate in seed pigmentation by RT-PCR analysis for gene expression in seed coat between a yellow-seeded line and its brown-seeded near-isogenic lines (NILs) , . In B. rapa, the TRANSPARENT TESTA GLABRA 1 (TTG1)  and the TRANSPARENT TESTA 8 (TT8)  encoding a WD-40 regulatory protein and a basic Helix-Loop-Helix transcription factor respectively, are suspected to be involved in seed pigmentation. These results suggest that Brassica species synthesize PAs and control the final seed color in a manner analogous to Arabidopsis. However, the molecular mechanism underlying seed color is not fully understood in Brassica. It is also worth noting that the allotetraploid B. juncea and B. napus and even the mesopolyploid B. rapa have much larger and more complex genome than Arabidopsis. As such, for a single-copy gene in Arabidopsis, there may be multiple copies in Brassica species. For instance, ANR  and TT16  each has four copies, and DFR two copies  in B. napus. To date, only a few flavonoid biosynthetic genes have been completely cloned from Brassica species .
Recently, next-generation sequencing (NGS) technologies have emerged as a revolutionary and high throughput approach that accelerates the genome sequencing and gene function research , . NGS-based RNA-seq can provide massive sequences with enormous depth and coverage to easily discover novel gene, splice junctions, fusion transcripts and to make a comparison of gene expression between samples or tissues , . It has been applied to analysis of transcriptomes of dozens of plant species including B. napus . However, RNA-seq has not been used to study B. juncea seed coat up to now.
In this study, two cDNA libraries generated from RNA of seed coat of a Brassica juncea yellow-seeded inbred line and its brown-seeded near-isogenic line were sequenced using Illumina/Solexa platform and de novo assembled into unigenes. The expression level of unigenes was compared between two libraries on the basis of read per exon kilobase per million (RPKM) analysis. 1,304 unigenes were found to be differentially expressed by at least 2-fold. Forty six unigenes were identified for flavonoid biosynthesis by KEGG analysis. The PA biosynthetic genes such as DFRs, LDOXs and ANRs were found to be significantly down-regulated in the yellow-seeded testa as compared with its black-seeded counterpart, as confirmed by qRT-PCR analysis. These results imply the involvement of PA biosynthetic genes in seed pigmentation in B. juncea.
Results and Discussion
Seed coat transcriptome sequencing and de novo assembly
Total RNA was extracted from seed coat of the Brassica juncea yellow-seeded inbred line Sichuan Yellow Seed (SY) and its brown-seeded near-isogenic line A (NILA, BC8F5) 15 days after pollination (DAP) . The phenotypes of the mature seeds of these lines are shown in Figure 1. A cDNA library was constructed for SY and NILA seed coat, respectively. These cDNA libraries were sequenced by using Illumina HiSeq™ 2000, generating 60,371,476 and 57,465,530 reads of 100-bp in length, respectively. After removing adaptor sequences, low-quality and ambiguous reads, 59,735,444 and 56,423,676 high-quality reads were obtained. An overview of the sequencing and assembly is listed in Table 1. All the raw transcriptome data have been deposited at the sequence read archive (SRA) of the National Center for Biotechnology Information (NCBI). By using the software CLC Genomic Workbench 4.9 (CLC Bio, Denmark), a total of 116,159,120 high-quality reads were assembled into 99,096 contigs, with a minimum contig size of 200 bp, a maximum size of 10,232 bp, an N50 of 1,060 bp and an average contig length of 664 bp. By performing the pair-end joining and gap filling, a total of 79,520 scaffolds were produced, with a minimum scaffold size of 200 bp, a maximum size of 12,040 bp, an N50 of 1,284 bp and an average length of 859 bp. The size distribution of the scaffolds is shown in Figure 2A. By using the software CD-HIT (V.4.5.4) , the scaffolds were further assembled into 69,605 unigenes, with a minimum scaffold size of 200 bp, a maximum size of 12,040 bp, an N50 of 1,307 bp and an average length of 868 bp. The size distribution of the unigenes is shown in Figure 2B.
(A) Sichuan Yellow Seed (SY); (B) The brown-seeded near-isogenic line(NILA) of SY.
(A) The size distribution of the scaffolds; (B) The size distribution of unigenes.
For functional annotation of B.juncea seed coat transcriptome, the unigene sequences were blasted against NCBI non redundant (Nr) protein database using a cut-off E-value of 10−5. A total of 49,758 unigenes (71.5% of all the assembled unigenes) were aligned to Nr protein database. Among these aligned unigenes, 62.01% had an E-value of less than 1.0E−50 and showed very strong homology while the remaining 37.99% had an E-value of between 1.0E−5 to 1.0E−50 (Figure 3A). The similarity distribution showed that 50.06% of these aligned unigenes had a similarity higher than 90%, 46.21% between 60% and 90% and only 3.72% lower than 60% (Figure 3B). For species distribution, approximately 96.49% of these aligned unigenes were matched with sequences from 6 top-hit species, i.e., Arabidopsis lyrata (46.59%), A. thaliana (40.59%), Thellungiella halophila (3.37%), B. rapa (2.38%), B. napus (1.87%), and B. oleracea (1.69%), which all fall into Brassicaceae. The 20 top-hit species based on Nr annotation are shown in Figure 3C.
(A) E-value distribution of the top Blastx hits against the non-redundant (Nr) protein database for each unigene; (B) Similarity distribution of the best Blastx hits for each unigene; (C) Number of unigenes matching the 20 top species using Blastx in the Nr database.
Gene Ontology (GO) classification
GO classification based on sequence homology revealed that 19,618 out of all the assembled unigenes were categorized into 37 functional groups (Figure 4). The three major categories (biological process, cellular component, and molecular function) were assigned 31,026, 22,918 and 26,267 GO terms, respectively (Figure 4). In the ‘biological process’ category, the unigenes related to ‘metabolic processes’ (58.85%) and ‘cellular processes’ (55.05%) were predominant while in the ‘cellular component’ category, ‘cell parts’ (42.05%) and ‘cell’ (42.05%) were found to be the most abundant class. Under the ‘molecular function’ category, the majority of unigenes were involved in ‘binding’ (63.28%) and ‘catalytic activities’ (55.09%).
COG classification of 49,758 Nr hits indicated that 25,140 unigenes were clustered into 25 functional categories (Figure 5). ‘Signal transduction mechanisms’ (10,471 unigenes, accounting for 41.6%) was found to be the major COG category, followed by ‘Posttranslational modification, protein turnover, chaperones’ (8,356, 33.2%), ‘General function prediction only’ (7,519, 29.9%), ‘Intracellular trafficking, secretion, and vesicular transport’ (4,081, 16.2%), ‘RNA processing and modification’ (3,921, 15.6%). ‘Cell motility’ (17, 0.01%) was the smallest group.
Analysis of 69,605 unigenes through Kyoto Encyclopedia of Genes and Genomes (KEGG)  showed that 14,998 unigenes were assigned to 258 pathways(Table S1). The major pathways containing hundreds of unigenes were metabolic pathways (3,506 unigenes, accounting for 23.37%), biosynthesis of secondary metabolites (1,785, 11.9%), microbial metabolism in diverse environments (802, 5.35%), RNA degradation (538, 3.59%), and ribosome (535, 3.57%). We concentrated on the ‘biosynthesis of secondary metabolites’ pathway in relation to seed pigmentation and found 154 unigenes for ‘Phenylpropanoid biosynthesis’, 114 for ‘Phenylalanine, tyrosine and tryptophan biosynthesis’, 46 for ‘Flavonoid biosynthesis’ and 9 for ‘Flavone and flavonol biosynthesis’ in B.juncea seed coat.
Identification of transcription factors in the seed coat transcriptome
All the assembled unigenes were aligned against the AGRIS (Arabidopsis Gene Regulatory Information Server) database by Blastx with E value of below 10−5 and identity of over 70%. A total of 2,347 unigenes were indentified to belong to forty eight putative transcription factors (TF) families (Table S2). For MYB and bHLH families, two major TF families whose members regulate flavonoid biosynthesis in plants , , , 100 and 190 unigenes were identified, respectively.
Transcripts differentially expressed between the yellow- and brown-seeded testa
To investigate the expression level of unigenes in the yellow- and brown-seeded testa, the number of clean reads was compared between the libraries for each of 69,605 assembled unigenes through RPKM analysis . 1,304 unigenes were found to be differentially expressed between the yellow- and brown-seeded testa, among which 802 unigenes were up-regulated while 502 down-regulated in the brown-seeded testa as compared to the yellow-seeded counterpart (Figure 5; Table S3). Among these unigenes, 170 (12.8%) showed over 15-fold change in expression level as well as 471 (36.4%) showed 2∼3-fold change. The fold change distribution of unigenes differentially expressed between the testae was shown in Figure 6. Annotation of differentially expressed unigenes revealed that 455 unigenes belonged to 28 GO groups while the remaining 849 unigenes could not to be classified (Figure 7).
Genes from the seed coat transcriptome involved in the flavonoid biosynthetic pathway
In Arabidopsis, PA synthesis initiates in the endothelial cells of seed coat at 3 DAP although expression of the genes for PA biosynthesis gradually decreases from 3 DAP onwards . In B. juncea, PA accumulation does not occur in seed coat until 10 DAP . Array analysis showed that six flavonoid genes (chalcone synthase (CHS), flavonoid 3-hydroxylase (F3H), flavanol (quercetin) 3-O-methyltransferase (FOMT), DFR, glutathione S-transferase (GST), and TTG1) had higher and two (flavonoid 3′-hydroxylase (F3′H) and flavonol synthase (FLS)) had lower expression in the developing siliques (22 DAP) of the B. carinata brown-seeded accession than in those of the yellow-seeded one . Comparison between secondary-wall-enriched seed coat and developing hypocotyls showed that the transcripts of flavonoid biosynthetic genes ANR, FLS and CHS were more abundant in the middle fraction of seed coat than in the developing hypocotyls of B. napus . This mean that flavonoid biosynthetic genes are expressed in seed coat at a higher level, which is consistent with sole deposition of PAs in seed coat . In this study, we used the seed coat for analysis, which eliminates interference from the silique wall or even the embryo. As mentioned above, forty six unigenes were found to be involved in flavonoid biosynthesis of B. juncea seed coat. Among them, six unigenes for CHS, and both two unigenes for chalcone isomerase (CHI) were up-regulated and one unigene for FLS down-regulated in the brown-seeded testa as compared to the yellow-seeded one(Figure 8; Table S4). Previous RT-PCR analysis and activity assay of three enzymes DFR, LDOX and ANR for PA biosynthesis have shown that DFR and LDOX are not expressed in seed coat of Brassica yellow seeds , , . Here, we found no DFR unigenes in the yellow-seed testa, but three DFR unigenes expressing at a high level in the brown-seeded testa with 10,510, 9,644 and 5,203 reads, respectively (Table S4). Similarly, three ANR unigenes were almost not expressed in the yellow-seed testa, although expressed highly in the brown-seeded testa. In addition, two LDOX unigenes were expressed at a much higher level in the brown-seeded testa than in the yellow-seeded one. Taken together, these results suggest that the genes for PA biosynthesis be not expressed or at a very low level and therefore no PAs accumulated in the yellow-seeded testa of B.juncea.
Abbreviation: ANR, anthocyanidin reductase; CHS, chalcone synthase; CHI, chalcone isomerase; DFR, dihydroflavonol 4-reductase; F3H, flavanone 3-hydroxylase; F3′H, flavonoid 3′-hydroxylase; FLS, flavonol synthase; LDOX, leucoanthocyanidin dioxygenase.
Real-time PCR analysis of the genes involved in the flavonoid biosynthesis pathway
To confirm the difference in expression level between the accessions found in RPKM analysis, eight unigenes for flavonoid biosynthesis were chosen for qRT-PCR analysis (Figure 8). These unigenes were Unigene_920 (CHS), Unigene_29246 (CHI), Unigene_7597 (DFR), Unigene_7701 (LDOX), and Unigene_16036 (ANR) which were up-regulated in the brown-seeded testa, Unigene_28310 (FLS) down-regulated, Unigene_682 (F3H) and Unigene_396 (F3′H) unchanged. qRT-PCR data confirmed expression pattern of these unigenes by RPKM analysis (Figure 9, Table S4).
To the best of our knowledge, this study is the first to apply the Illumina/Solexa platform to investigating the sequences and transcript abundances of genes expressed in seed coats of Brassica juncea. This transcriptome analysis has provided a total of 69,605 unigenes among which 71.5% are aligned to Nr database although there is no Brassica juncea reference genome sequence of available. Comparison in expression level between the yellow- and the brown-seeded testa totally identified 1,304 differentially expressed unigenes including the key PA biosynthetic genes such as DFRs, LDOXs and ANRs. In sharp contrast to high expression in the brown-seeded testa, expression of the PA biosynthetic genes and accumulation of PAs are inhibited in yellow-seeded testa, which suggests that PAs play a major role in seed pigmentation of B. juncea. The unigene sequences obtained in this study will not only lay the foundations for insight into the molecular mechanism underlying seed pigmentation in B.juncea, but also provide the basis for further genomics research on this species or its allies.
Materials and Methods
A yellow-seed inbred line Sichuan Yellow Seed (SY) and its brown-seeded near-isogenic line A (NILA) (Figure 1) of Brassica juncea (L.) Czern & Coss were used in this study. The plants were grown in a greenhouse under a photoperiod of 16 h day and 8 h night at 22°C. Fresh seed coats were excised by hand from the seeds 15 days after pollination, immediately frozen in liquid nitrogen, and stored at −80°C for RNA extraction.
Construction of cDNA library for sequencing
Total RNA was extracted from seed coats using TRIzol Reagent (Life technologies, US) following the manufacturer's instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, US). Qualified total RNA was purified by RNeasy micro kit (QIAGEN, Germany) and RNase-Free DNase Set (QIAGEN, Germany). Poly(A) mRNA was isolated from qualified total RNA using Oligotex mRNA mini kit(QIAGEN, Germany) and then broken into short fragments which were used as templates for synthesis of the first- and the second-strand cDNA. Two paired-end libraries were synthesized by using the Genomic Sample Prep kit (Illumina, US) per the manufacturer's instructions. Short fragments were purified with the Qubit™ dsDNA HS Assay kit (Invitrogen, US) and connected with different sequencing adapters. Each of the two libraries had an average insert size of 400 bp and was sequenced by Shanghai Biotechnology Corporation (Shanghai, China) using Illumina HiSeq™ 2000. The transcriptome datasets are available at the NCBI Sequence Read Archive (SRA), under the accession number SRA072246.
De novo assembly
Pre-processing and assembling of the raw sequence data were carried out by using CLC Genomics Workbench (version 4.9). This pre-processing included the removal of the adapter sequences, low quality (Q20<20) sequences, shorter-than-15-nucleotide sequences and ambiguous inner regions. For de novo assembly, contig scaffolding algorithm was adopted. The parameters were set as follow: length fraction (i.e. minimum length fraction of a read that must match the reference sequence) of 0.5, similarity ratio (i.e. minimum similarity of the match length of a read) of 0.8 and the minimum contig length (minimum length of an assembled contig to be reported) of 200 bp or longer. Then, the scaffolds were clustered by using CD-HIT (V.4.5.4) with 90% similarity cut-off to produce distinct unigenes.
Function of unigenes was annotated by Blastx against the NCBI non-redundant (Nr) database or the AGRIS database (http://arabidopsis.med.ohio-state.edu/downloads html)  with E-value threshold of 10−5. Base on Nr annotation, top-hit species were identified. Gene ontology (GO) classification was obtained by WEGO  (http://wego.genomics.org.cn/cgi-bin/wego/index.pl) via GO id annotated by Perl and R program. The unigenes sequences were also aligned to the COG database to predict and classify functions. KEGG pathways were assigned to the unigene sequences using the single-directional best hit (SBH) method on the KEGG Automatic Annotation Server (KAAS) online .
The RPKM method was used to calculate the unigene expression in this study. The clean reads of each library were mapped to the sequences of each unigene. The significance of difference in gene expression between the yellow- and brown-seed testa was determined by using DEGseq, an R package . False discovery rate (FDR) was applied to identify the threshold of the P value in multiple tests . When FDR is less than 0.05 and log2 ratio greater than 1 (two-fold change) between the accessions the unigenes were considered as differentially expressed.
Real-time quantitative RT-PCR(qRT-PCR) analysis
Eight unigenes involved in flavonoid synthesis were chosen for validation by qRT-PCR. The primers designed with the software Primer Premier 5.0 were listed in Table S5. Total RNA was extracted from seed coat tissues 15 DAP using TRIzol RNA extraction method (Tiangen, China) and reverse transcribed into cDNA using PrimeScritH RT reagent kit with gDNA Eraser (Perfect Real Time) (Takara, China). The qRT-PCR was performed with a Bio-Rad CFX-96 RealTime PCR System (Bio-Rad, US) in a final volume of 20 µl containing 2 µl cDNA, 10 µl 2×SYBR premix Ex taqTM (Takara, Japan), 0.4 µl 10 µM the forward and reverse primers each, and 7.2 µl RNase-free water. The thermal cycling conditions were as follows: 95°C 5 min, 45 cycles at 95°C for 5 s for denaturation and 56°C for 25 s for annealing and extension. TIPS-41  was used as an internal control for normalization to make a comparison of gene expression level between the accessions.
Summary of the KEGG pathway and their corresponding gene number in the transcriptome of Brassica juncea seed coat.
Transcription factors identified in the transcriptome of Brassica juncea seed coat.
The unigenes differentially expressed between between the yellow- and brown-seeded testa of Brassica juncea.
The unigenes involved in flavonoid biosynthetic pathway and their coverage in the transcriptome of Brassica juncea seed coat.
We acknowledge the Shanghai Biotechnology Corporation for technical assistance in data processing and bioinformatics analysis.
Conceived and designed the experiments: XL CG ZL. Performed the experiments: XL YL YY SL. Analyzed the data: XL YL YY. Contributed reagents/materials/analysis tools: SC CG XL. Wrote the paper: XL ZL.
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Lakeville City Councilman Luke Hellier Announces His Campaign for Lakeville Mayor – Moving Lakeville Forward by focusing on public safety, rebuilding our economy, and promoting responsible growth
(LAKEVILLE, MN) – Today, Lakeville City Councilman Luke Hellier announced his campaign for Lakeville Mayor. Hellier was elected to City Council in 2016 and is currently in the middle of his second term.
Hellier plans to continue efforts to responsibly manage Lakeville’s growth and assist in continued recovery following the COVID-19 pandemic.
“I am proud of how Lakeville – including its staff and leadership – weathered the storms of the last years which have fundamentally changed the ways Lakeville families rely on their community. They spend more time in their neighborhoods, eat and shop here more consistently, and depend on our parks and services in new ways. Our businesses face new challenges in response to COVID. We have deepened our conviction that police and fire are essential to the care of our entire community. Residents today expect more from their public servants and I plan to continue to lead on these issues to move Lakeville forward.”
As Lakeville has grown to the largest city in Dakota County and ninth largest in the state, Hellier has voted to expand Lakeville’s police force to ensure our community is the best place to live and raise a family. While on the council, Hellier has also championed consistent increase in support for the Fire Department through investing in equipment and advocating for boosting the retirement fund for the volunteer fire fighters.
Hellier added, “It’s been an honor and a privilege to serve the Lakeville community for the last five years on City Council. As Mayor I will build on our past successes and continue Lakeville’s legacy of leadership,” said Hellier.
Luke Hellier attended Lake Marion Elementary School, Kenwood Trail Junior High School, and is a graduate of Lakeville High School. Hellier previously served on the Dakota County Planning Commission and ISD194 Long Term Facilities Planning Committee. He’s also an active volunteer with the Lakeville Public Safety Foundation. Luke works for a public relations firm in Minneapolis and lives near Crystal Lake with his wife, Josi, and children Betty, Annie, and Walter. For more information visit www.votelukehellier.com.


text:
Ljunggren (Younggren), Christian Andersson (3)
B: 12 Feb 1795, at Horrod, Bronnestad as the son of the juryman Anders Waldermarsson and his wife, Boel Andersdotter at Horrod.
D: 1859 It has been said that he was buried under a big tree, a gravesite undoubtedly lost.
M: 22 Mar 1829, Pernilla Persdotter b. 22 Feb 1799 at Spangmollan, Grydt to Par Jonsson and Ingar Jonsdotter. d. 1854. M: In the Parish of Stoby. On the voyage across the ocean to America, Pernilla contracted Cholera, died and was buried at sea.
Christian was a farm owner while he lived in Sweden
FN: Anders Wollermarsson
MN: Boel Andersdotter
Lineage: Wollermarsson, Anders (2); Hansson, Wollemar (1)
Children of Christian Andersson Ljunggren and Pernilla Persdotter:
Bengta, Sven, Axel, Nils (All born in Parish of Stoby, Province of Skane)
1. Bengta Younggren
B: 28 Feb 1830
C: 23 Jun 1844 The inscription in Bengta's confirmation Bible reads "Bengta Christiansdotter at number 3 Ignaberga got this book June the 23rd, 1844 at the Church of Wankifva in her alliance of christening for her confirmation in public." The 5th book of Mose 29: 10,11,12 and 13. Lukas 14: 17.
D: 16 Dec 1870
M: Carl A. "Charlie" Byerstedt (1829 - 1906)
Children: Inga, Louis, Paula, Carl William (5)
2. Sven Younggren (4)
B: 09 Sep 1833 Parish of Stoby, Province of Skane, Sweden
D: 27 Sep 1899
M: 05 Nov 1868 Hanna Nelson b. 12 Jul 1846 d. 05 Feb 1908
Children: Nelson Amos, Alfred Henning, Bengta Delia, Sylma, Axel Oliver (5)
3. Axel Younggren (4)
B: 09 Jan 1836 Parish of Stoby, Province of Skane, Sweden
D: 23 Feb 1917
M: 16 Apr 1858 Malinda Mattson b. 30 Aug 1840 d. 12 May 1927
4. Nils Younggren (4) As recorded
in Swedish Archives in Lund, Skane, Sweden
B: 08 Jul 1838 Allgutstorp, Stoby
D: 02 Nov 1841
It is believed that Christian adopted the name of Ljunggren sometime in his early days when serving in the Swedish army. It is said that when these young men entered their army service they took another name, one of their own choice, that were called fun names and continued to use them after their tour of duty. Christian took the name of Lunggren which means heather branch. Ljung (heather) is a small evergreen bush that grows profusely in southern Sweden and "gren" means branch, hence the name Ljunggren. The name became Younggren soon after the family moved to Minnesota Territory in 1854 because in Minnesota the name was often spelled more like it sounds. One source says family emigrated to North America 15 Mar 1854.
Christian and his children arrived in America on or about August 1, 1854 according to the Naturalization Certificate of Swen Younggren that was dated September 15, 1854.

domain: history


There are approximately 55 million Tweets sent per day on Twitter, that equates to 640 tweets per second!
It would take a lifetime to manually search Twitter for the tweets that may be of any interest or relevance to you. TweetDeck is a handy tool that can help you search for what you want and filter out what you don’t.
Whether you want to keep track of a trending news story, follow mentions of your brand, monitor conversation around your key words and services or even keep tabs on the competition, Tweetdeck is a great tool to do it.
To get started using TweetDeck head to www.tweetdeck.com and download TweetDeck for your desktop
Once Tweetdeck is installed, open it up and hit the ‘get started’ button. Please note, if you don’t currently have an active Twitter account you will have to create one at www.twitter.com (this shouldn’t take more than a few minutes)
At the welcome page, add your Twitter account by clicking ‘add twitter’. Enter your login details and hit ‘submit’. The next window will ask you if you want to register your Tweetdeck account – you can skip this step for now.
You should now have Tweetdeck open infront of you complete with a few default columns, these will include ‘all friends’ ‘mentions’ and direct ‘messages’ – keep these open as they are useful for monitoring your account.
There will also be two more columns, ‘trending worldwide’ and ‘TweetDeck recommends’ – These aren’t so important so can be deleted. To do this, hover over the ‘t’ Twitter logo located at the top right of the column and hit the ‘x’:
Now we can set up some search columns. To do this click the add column button at the top of the page:
Lets say for example, we want to follow conversations about the new search engine, Blekko. Simply key in ‘Blekko’ and hit ‘Search’:
We will now have a column that displays a feed of all tweets containing the word ‘Blekko’:
If you wish to search for more than one keyword, use the following parameters:
- AND will search for both search terms (or more if used)
- OR will for either one or the other search term (or terms)
- Use ‘quotes’ to search for a specific search phrase
Please note: When using AND or OR they must be used in CAPS.
If you find that a certain search term is churning up a lot of spam or irrelivant tweets, you can delete the column in the same way we did before. Alternatively, you can edit the search from within the column. To do this, hover your cursor over the top of the column and click ‘edit’, enter your required search and hit save:
If you don’t have the time to monitor all your search results, TweetDeck has a notification system that will alert you everytime a relevant search term appears. To set up notifications, click the ‘settings’ button, located in the top-right corner of TweetDeck and click ‘notifications’ from the menu
Click ‘Advanced options for columns’ and using the tick boxes, select what columns you want alerts for. On this page you can also select where you want the notification to appear on your desktop, how much information you want to see in the ‘pop up’ and whether you want it to make a sound or not. We suggest setting up notifications for only the most important of your search columns as eventually, beeps and pop-up messages can become a little distracting.
TweetDeck can look a little confusing at first, but once you start to explore and make sense of it, it can be a very useful search tool.
For any questions about using TweetDeck please contact us.


text:
Mikel Oyarzabal scored the winning penalty in the shootout to break Swiss hearts.
Spain will play either Italy or Belgium in the first semi-final at Wembley next Tuesday.
The Spanish toiled for long periods against Switzerland and wasted several good chances to win the tie in extra time before they limped over the line 3-1 on penalties.
Spain had earlier gone ahead after just eight minutes when a shot from Jordi Alba from distance took a huge deflection off Denis Zakaria and past the helpless Yann Sommer.
Zakaria was only in the team in place of the suspended Switzerland captain Granit Xhaka.
Spain shot themselves in the foot in the second half when a defensive mix-up between Aymeric Laporte and Pau Torres gifted an equaliser to Xherdan Shaqiri on 68 minutes.
Switzerland were reduced to 10 men on 78 minutes when Remo Freuler was shown a straight red card for a late challenge on Gerard Moreno.
Moreno wasted a glorious chance in the first half of extra time and Spain missed several good opportunities to win the game
But a mixture of wasteful finishing, inspired goalkeeping from Zommer and heroic Switzerland defending meant the game went to penalties.
Sergio Busquets missed the first penalty in the shootout but Fabian Schar, Manuel Akanji and Ruben Vargas missed for Switzerland before Oyarzabal won it for Spain.

domain: sports


text:
Enjoy a comfortable stay at the modern Holiday Inn Cagliari hotel, a few minutes' drive from Cagliari-Elmas Airport and Cagliari's historic centre.Vibrant contemporary paintings brighten Holiday Inn Cagliari's stylish Lobby, where you can check emails with wireless Internet. Refresh with a swim in our outdoor pool and settle into your homely Guest room with satellite TV. We're a good base for early and late flights, just 4km from Cagliari-Elmas Airport, and close to Sardina's key SS131 and SS554 motorways.Do business with nearby companies such as Coca Cola and invite up to 350 people to an event in Holiday Inn Cagliari's 8 air-conditioned meeting rooms. Our friendly staff can help set up high-tech conference equipment and arrange refreshments in our sunny break-out spaces.In downtown Cagliari, 4km away, eye up the catch of the day in bustling San Benedetto fish market and hear tales of gladiator duels on a tour of the limestone Roman Amphitheatre. White sandy beaches invite relaxation at Poetto, 8km from Holiday Inn Cagliari.The hotel's American-style Le Saline cocktail bar serves drinks until late in sleek surroundings, and you can dine on flavoursome Mediterranean cuisine on tables set with crisp white linen in Vecchia Darsena Restaurant. Keep fit in our MiniGym and unwind with an indulgent sauna and steam bath.
Details / Other Expenses
|Year of Last Renovation:||2005|
|Corridors:||All Rooms Have Interior Hallways|
|Non Smoking Rooms:||0|
|Rooms with 1 Bed:||88|
|Room Windows:||Windows Do Open|
|Meeting Rooms:||0 |
|Meeting Space:||1607 sq. feet| Policies / Expenses
|Typical Group Deposit:||30%|
|Total Misc Fees:||$0.00 USD |
(mandatory resort fees/taxes)
|VAT: Value Added Tax:||10.00 VAT % Inclusive|
Amenities / Features Pet Friendly? Sorry, pets are not allowed.
Airport & Shuttle Information
Elmas Cagliari Airport
Airport Shuttle? Sorry
airport shuttle available.
About Holiday Inn
- Full-service hotel offering three different experiences: Holiday Inn Express ideal for the business traveler, Holiday Inn Resorts for family fun and relaxation, and the Holiday Inn Club Vacations, suitable for family and friends with spacious accommodations
- Offering comfort and family-oriented service, children stay and eat free at Holiday Inn properties
- Free high-speed internet access
- Restaurants and bars on-site with good food and drinks for all ages
- Indoor or Outdoor swimming pools based on location
- Great exercise facilities
- Based on select properties, Holiday Inn's The Hub is the perfect entertainment spot and gaming area with a media lounge, fire pits, etc.
The Holiday Inn highlights above are subject to change without notice.

domain: travel


Term Rewriting and Rewriting Logic
Our interest in term rewriting originates in the fact that we believe term rewriting is a powerful programming paradigm in general, especially useful in giving semantics to languages. That is because a runing step in the execution of a program can be seen as the evolution of a state. If one thinks of the state as being a term, rewrite rules provide means of specifying how the state should evolve.
Two are our main goals regarding term rewriting:
- Researching algorithms and methods which would take advantage of the parallel nature of rewriting. We have developed a method to transform conditional rewrite systems into unconditional ones, since the former are harder to handle in a parallel execution environment. For more information, see Computationally Equivalent Elimination of Conditions
- Research ways for efficiently generating interpretors and compilers from (rewriting-based) language definitions. We hope that due to the specifity of the domain, optimizations that cannot be applied when interpreting/compiling general rewrite systems, could be used to get very fast interpretors for languages from their formal specification. Preliminary results in this direction can be found in A Rewrite Framework for Language Definitions and for Generation of Efficient Interpreters
A rewrite logic theory consists of a set of uninterpreted operations constrained equationally, together with a set of rewrite rules meant to define the concurrent evolution of the defined system. The distinction between equations and rewrite rules is only semantic. They are both executed as rewrite rules by rewrite engines, following the simple, uniform and parallelizable match-and-apply principle of term rewriting: find a subterm matching , say with a substitution , then replace it by . Therefore, if one is interested in just a dynamic semantics of a language, then, with few exceptions, one needs to make no distinction between equations and rewrite rules.
In our context of programming languages, a language definition is a rewrite logic theory in which equations define the non-concurrent features of the language, while rewrite rules define the concurrent features. A program together with its initial state are given as an uninterpreted term, whose denotation in the initial model is its corresponding transition system. Depending on the desired type of analysis, one can, using existing tool support, generate anywhere from one path in that transition system (e.g., when “executing” the program) to all paths (e.g., for model checking).
In spite of its simplicity and generality, rewriting logic does not give us any immediate recipe for how to define languages as rewrite logic theories. Appropriate definitional techniques and methodologies are necessary in order to make rewriting logic an effective computational framework for programming language definition and formal analysis.
Starting from the broader subject of Rewriting Logic Semantics we are currently developing K, a definitional technique and methodology on top of Rewriting Logic, specifically aiming at Programming languages definitions, which are both operational and denotational.