text:
Welcome to Episode 148 of Building My Legacy.
In this podcast, we talk with Martin Saenz, a thought leader in the mortgage note investment industry. If you’re not familiar with how mortgage notes can provide reliable cash flow — according to Martin, an 8 percent yield — while helping homeowners who are in default stay in their homes, you’ll find our discussion extremely informative. Martin talks about how he discovered mortgage notes when he no longer wanted to work up to 100 hours a week at his own business. He explains how this niche industry can deliver dependable cash flow to someone who’s accredited and has as little as $50,000 to invest. The information Martin has to share is valuable for business owners, entrepreneurs and those of you looking toward that next chapter of your life, which may involve starting a not-for-profit or giving back to your community.
So if you want to know:
- About a company that makes money while fulfilling its mission: to avoid foreclosure and keep families in their homes
- How the mortgage note investment industry works
- How the secondary mortgage market is affected by an economic downturn
- Why you should have a “living financial statement”
- Why you need assets that provide cash flow that you have control over
About Martin Saenz
Martin Saenz, a managing partner of Bequest Funds, co-founded this company with the dual purpose of helping investors grow their wealth while helping mortgage borrowers stay in their homes. A successful entrepreneur and real estate investor for more than 15 years, Martin brings social good into smart investing. He is the author of several books on note investing: Note Investing Made Easier, Real Estate Note Investing Mentorship, Note Investing Fundamentals and Cashflow Dojo. Martin has directly helped more than 1,000 families stay in their homes and countless more through the influence of his mentorship. More information is available at his company website, www.bqfunds.com
About Lois Sonstegard, PhD
Working with business leaders for more than 30 years, Lois has learned that successful leaders have a passion to leave a meaningful legacy. Leaders often ask: When does one begin to think about legacy? Is there a “best” approach? Is there a process or steps one should follow?
Lois is dedicated not only to developing leaders but to helping them build a meaningful legacy. Learn more about how Lois can help your organization with Leadership Consulting and Executive Coaching:
Thanks for Tuning In!
Thanks so much for being with us this week. Have some feedback you’d like to share? Please leave a note in the comments section below!
If you enjoyed this episode, please share it with your friends by using the social media buttons you see at the bottom of the post.
Don’t forget to subscribe to the show on iTunes to get automatic episode updates.
And, finally, please take a minute to leave us an honest review and rating on iTunes. They really help us out when it comes to the ranking of the show, and I make it a point to read every single one of the reviews we get.
Please leave a review right now. Thanks for listening!
Recent episodes from Mike Saunders, MBA (see all)
- Interview with Tim Tacl Co-Founder / Sr. Loan Officer at DNVR Lending - November 30, 2023
- Episode #53 – Charitable Giving – The 15 Minute Financial Feast Podcast-With Mark Triplett & Troy Westendorf - November 29, 2023
- Kim Magdalein w/Magdalein Financial-How Social Security Planners of America Helps Advisors Reimagine Social Security for their Clients - November 28, 2023

domain: finance


text:
EL RENO — A judge Thursday allowed attorneys for a man accused of fatally strangling his former girlfriend and her four children to withdraw from the case.
Canadian County District Judge Gary Miller granted a request from lawyers with the Oklahoma Indigent Defense System to no longer represent Joshua Steven Durcho, 27, after the lawyers declared a conflict of interest.
While no indication of the conflict was given in court or by attorneys, the action came almost two months after Durcho and another murder defendant allegedly had a failed escape attempt from the Canadian County jail. Durcho and Jeremy Alan Pendleton, who also was represented by Oklahoma Indigent Defense attorneys at the time, were really never a threat to escape, Sheriff Randall Edwards said.
While ruling Thursday a conflict of interest had developed between defense attorneys and Durcho, Miller declined to postpone the Sept. 13 trial.
Miller said he would consider changing the trial date after new attorneys are appointed.
Durcho has pleaded not guilty in the Jan. 12, 2009, killings of Summer Rust; her daughters, Kirsten Rust, 7, Autumn Rust, 7, and Evynn Garas, 3; and her son, Teagin Rust, 4.
Prosecutors are seeking the death penalty for Durcho, who appeared at the hearing in shackles, an
Defense attorneys and the prosecution declined to comment after the hearing. Court filings on the matter have been placed under seal and are not publicly available.
The entire Oklahoma Indigent Defense Capital Division in Norman has declared the conflict.
No indication of who would be Durcho's next attorney was given, although the public defender system does have a capital division in the Tulsa area and also contracts with private attorneys qualified to handle death penalty cases.
Judge Miller said the conflict arose after the state filed an amended bill of particulars.
The original bill of particulars stated several reasons why prosecutors think the death penalty is warranted.
The amended bill of particulars was filed under seal on July 9.
The court document was filed after Durcho and Pendleton allegedly tried to escape on June 24. A day earlier, Miller denied defense attorneys' request to move the trial to another location because of pretrial publicity.
Pendleton was trying to remove mortar from a wall so he could get into Durcho's adjacent cell, Edwards said.
Durcho was doing the same in another wall that adjoins an exterior exercise area.
The wall is steel reinforced, and the exercise area was surrounded by a tall security fence.
"It wasn't anything of any threat to security or anything where they were even close to getting out," Edwards said.
"They weren't going anywhere if they had removed the mortar."
At the time of the escape attempt, Oklahoma Indigent Defense System attorneys from the capital division also represented Pendleton, according to court records.
Pendleton is charged in connection with the stabbing death of an 18-year-old in El Reno on March 28.

domain: news


text:
Global Aviation Holdings (Global), a leading provider of charter air transportation, commercial global passenger and cargo air transportation services, and Lufthansa Technik AG have extended their existing cooperation for Global's wholly owned subsidiaries World Airways (World) and North American Airlines (North American).
The two companies have signed a new Total Component Maintenance TCM contract for up to nine MD11 from World and a Total Component Support TCS® for the five Boeing 767-300ER of North American. Both contracts cover a period of five years.
"Lufthansa Technik's expertise to globally support our two airlines, in conjunction with the ability to adapt the program to the specific needs of both World Airways and North American Airlines, made LHT our provider of choice," said Charlie Addison, Senior Vice President, Global Shared Service (subsidiary of Global).
Robert Gaag, Vice President Marketing and Sales North America from Lufthansa Technik, added: "The contracts with Global Aviation Holdings are the latest proof for our abilities to offer appropriate support in the very special air transport and air cargo market. We are extremely proud that one of the worldwide leading air transportation companies has re-confirmed its trust in the quality of our technical services. This is a strong motivation for us to further
increase our activities in the very competitive U.S. market."
The new TCM is an extension to an existing TCS® with World to service three Boeing 747-400. The new contract comprises about 800 part numbers. Lufthansa Technik will mainly support World at its bases at Tampa International Airport in Florida/USA and at Leipzig/Halle Airport in Germany. Additionally, the two companies agreed on a logistics concept for a worldwide delivery of components.
The new TCS® for North American is an extension to the existing home base lease contract between the two companies for the Boeing 767 fleet. The TCS® includes about 1,100 part numbers. Lufthansa Technik will also focus its support for North American on the bases of the
airline at Tampa International Airport in Florida /USA and at Leipzig/Halle Airport in Germany, but will guarantee a global component delivery service, too.
Global Aviation Holdings
Global Aviation Holdings (Global), through its wholly owned subsidiaries World Airways (World) and North American Airlines (North American), is the largest commercial provider of charter air
transportation for the U.S. Military, and a major provider of worldwide commercial global passenger and cargo air transportation services. Global offers a combined fleet of 16 cargo and passenger aircraft and takes pride in being the leader in customized air transportation with its fleet of Boeing 747, MD11 and Boeing 767 aircraft.
North American Airlines
North American Airlines (North American) is a wholly owned subsidiary of Global Aviation Holdings. As a Part 121 fully certified United States Flag Air Carrier since 1989, North American provides exceptional air transportation services throughout the world. With a reputation for first-class technical and operational performance, North American operates a fleet of 5 Boeing 767-300ER (180 ETOPS) aircraft. North American provides worldwide charter operations for the U.S. Military, Tour Operators, Incentive Groups, Government Agencies, Sports Teams and other airlines.
World Airways (World) is a wholly owned subsidiary of Global Aviation Holdings. As a fully certified United States Flag Air Carrier since 1948, World has a long history of serving passenger, cargo and military customers across six continents. With a fleet of 11 wide-body Boeing 747, and MD11 aircraft, World has the equipment, experience and flexibility to meet the demands of major international airlines, freight forwarders, tour and cruise operators and government entities. World Airways is proud of its long relationship with the Air Mobility Command and serves as the largest commercial carrier of U.S. Military personnel.
The Lufthansa Technik Group, with more than 30 subsidiaries and associates and over 26,000 employees worldwide, is one of the leading manufacturer-independent providers of technical services for the aviation industry. Its portfolio encompasses the entire spectrum of services for commercial aircraft: maintenance, repair, overhaul, modification and refit, engines and components.
The two companies have extended and significantly expanded their existing Total Component Support TCS® agreement for the airline's fleet of Airbus A320 and Boeing 737 aircraft for 10 years.
Canada's largest airline has signed a 12-year Total Component Support TCS contract with Lufthansa Technik, starting with the delivery of the first aircraft from Boeing to Air Canada in the first...
Lufthansa Technik will maintain components removed from WestJet Encore aircraft and provide replacements from its extensive pool of Q400 stock.
Total Component Support TCS® for 34 Boeing 737NGs.

domain: geography


Thank you very much Chairman Ravitch and members of the Commission. I am pleased to appear before you this morning and very much appreciate the invitation.
I work for the Brookings Institution’s Metropolitan Policy Program, a nonprofit public policy organization based in Washington, DC. Our mission is to redefine the challenges facing metropolitan America and promote innovative solutions to help communities grow in more inclusive, competitive, and sustainable ways. I work on a range of policy areas with specific emphasis on transportation.
Mr. Chairman, the work of this commission comes at a critical time in our nation’s history. As you know, the issue of transportation funding, and transit funding especially, is very much on the minds of metropolitan area leaders from coast-to-coast. And for good reason.
There are massive changes underway with respect to how Americans travel. After years and years of steady increases, the total amount of driving in the U.S. has slowed down dramatically. In fact, monthly dispatches from the federal highway administration illustrate clearly that as a nation we are driving much less. Cumulative travel for 2008 has decreased by 42.1 billion vehicle miles, the largest drop in driving that this nation has ever seen.1
Without a doubt some of this decrease is attributable to skyrocketing gas prices which, although they have fallen in the last two months, are still one dollar per gallon higher than this time last year.
Partly as a result of the increase in gas prices and the decrease in driving, transit ridership is booming. The American Public Transportation Association recently announced that Americans took more than 2.8 billion trips on public transportation in the second quarter of 2008. This is almost 140 million more trips than last year.2 The New York MTA is responsible for 55 million of these trips.3
Unfortunately, we also know that transit agencies are not immune from the increases in fuel costs and at the same time are struggling to cope with this increased demand. Perversely, 35 percent of transit agencies are considering cuts in service as a result of the increased costs of energy.4 So at the time when the nation needs functioning, reliable, mass transit we are finding many agencies in severe distress.
Mr. Chairman, I mention all this to illustrate the need for innovation and national leadership when it comes to addressing the issue of transit funding.
Sufficient and sustained revenues for metropolitan transit systems, whether generated on a regional or statewide level, are justified given a series of oft-cited benefits that extend far beyond the inherent value of a soundly financed transit system.5
An extensive transit network like the New York MTA, for example, provides important transportation alternatives to those who have options and provides basic mobility for those who do not. It can help mitigate regional air-quality problems by lowering overall automobile emissions and slowing the growth in traffic congestion.6 And it can also provide economic benefits by creating development opportunities around transit stations and help enhance regional economic competitiveness as an important and attractive metropolitan amenity.7
In view of these broader impacts, then, there are a number of potential revenue sources for the MTA:
Gasoline taxes: Transit advocates in many metropolitan areas have promoted regional gasoline taxes as a way to finance transit. For many, gas taxes represent an attractive option because motorists already pay federal, state, and local taxes on motor fuel so the levy would not impose a new type of tax. To be sure, few transit agencies derive their dedicated funding from regional or metropolitan gas taxes (although the taxes are widespread on other jurisdictional levels). But even so such proposals continue to have merit because of their ability to reduce the externalities associated with automobile travel (e.g., congestion, pollution) and induce drivers to use vehicles that are more fuel-efficient.8 However, the long-term feasibility of gasoline taxes as a sustained source of revenues for transportation financing in general is increasingly being called into question.9
Sales taxes: By contrast, regional sales taxes are a very common form of dedicated funding for transit agencies. In the Dallas metropolitan area, for example, a 1-cent sales tax generates about $350 million each year for the transit agency there. In and around Chicago, a complex sales tax in the regional transit authority district generates more than $500 million for the agency. As others have pointed out, the key appeal of a dedicated sales tax for transit is that it has a broad base and significant resources can be raised with a barely noticeable change in the tax rate.10 The main disadvantages of sales taxes are that they are regressive unless modified, and they are not directly related to the use of transportation so they can be unpopular over a wide area. However, the significant amount of revenue this option raises, the relative ease in its administration, and its widespread and successful use to support transit throughout the nation make it a viable alternative.
Parking taxes. A related idea is that of a parking tax to fund transit. A parking tax was first proposed before construction even began on the rail system in Washington, for example.11 Such a source could take the form of a sales tax on commercial parking or an excise tax that all parkers pay. Parking taxes have the added advantage of discouraging vehicle trips and perhaps increasing transit’s share of total trips.12 Parking taxes are currently used in several U.S. cities and in almost every case the primary goal is revenue generation. Parking taxes for transit purposes are enabled by statute in states such as California and Illinois.
Land value capture: Value capture refers to a type of financing where non-transportation users, primarily adjacent property owners who benefit directly from transportation projects, contribute to the costs of such investments. It is often proposed as a way to use public taxing authority to help finance rail transit through taxes on nearby private development, in some ways mimicking the link between private rail transit and private land development a century ago. The idea is that rail systems, by improving accessibility, will increase land values. The increment over any pre-existing property value can then be taxed, thereby “capturing” the benefit that accrues to private landowners by virtue of the public rail investment, and helping to defray rail construction or operating costs. Though the idea of value capture has been discussed in policy circles for years it is employed in relatively few places and most prominently in the form of benefit assessment districts in metropolitan areas including Miami, Los Angeles, and Denver.13
Payroll taxes. Still another potential option is a payroll tax on workers’ earnings in the service area dedicated to covering local operating subsidies. Such a tax could be assessed based on place of employment rather than place of residence in order to expand the tax base to individuals working in the MTA transit district but residing outside of it. The transit district in Portland, OR is supported by such a tax, as are the transit agencies in Cincinnati and Louisville. A payroll tax closely tracks inflation, so revenues would remain quite stable. It can also be made progressive by exempting low-income workers.14
Mr. Chairman, in addition to these options, the one I believe holds the most promise for New York City and the transit agency is a congestion charge. In the words of Sir Rod Eddington—the author of a ground-breaking report on the future of Britain’s transportation infrastructure—congestion pricing is an “economic no-brainer.”15 The real and perceived benefits are the reason there is such a tremendous amount of attention focused on such strategies today.
Congestion and cordon charges. Charging commuters who travel on roads during peak hours is becoming a popular idea throughout the world. Although its applications in the U.S. have been rather limited, receptivity is growing as congestion continues to increase at the same time revenues are become scarcer. Moreover, new toll collection technologies enable charges to be collected without slowing down vehicles, thereby removing a significant barrier to implementing such a plan.
Congestion pricing comes in many forms and involves tolling one of more lanes of a particular roadway to reduce demand to the level of maximum efficient use of road capacity. Under some schemes pricing can vary based on time of day or amount of congestion. Congestion pricing can offer a number of benefits including increased access and revenue for public transit. According to the U.S. DOT, congestion pricing, if adopted in all areas of major congestion, could reduce the need for new roadway capacity by $20 billion.16
Under cordon (or area-wide) congestion pricing a fee is charged for any vehicle that enters the cordoned area, usually a city center. The justification is that vehicles entering the cordoned area impose costs (congestion, air pollution, carbon emissions, road damage, etc.) on the residents of the area that is unfair and cannot be supported by the local tax base. An additional argument is that dense city centers work most efficiently when public road space is allocated in a manner that best sustains overall quality of life. These lessons are evident internationally in cities like Singapore, Stockholm, Oslo, and London which have adopted cordon pricing schemes.17
Most relevant for the discussion of this Commission is that congestion pricing can, and usually does, yield sufficient net revenues to provide for additional bus and rail capacity, bicycle and pedestrian improvements, or projects to provide alternative transportation through other modes within the cordon zone. Yet one important point associated with these international strategies is that revenue generation is not the only objective of cordon-style pricing. The primary objectives and benefits begin almost uniformly with the desire to reduce congestion in the urban core by reducing automobile traffic. Other objectives include increased accessibility, and environmental benefits of reduced vehicle emissions.18
Despite these clear and oft-cited benefits, a critical factor in public acceptance for cordon pricing strategies is how the congestion charge revenues are spent. Complaints about the regressive nature of toll revenues remains a common concern, with low-income and high-income users paying the same price for access to the cordoned area. However, several models show that these and other similar charges could be considered through an expenditure lens, not an income lens. In other words, the equitable distribution of the toll revenue is more important than the equitable distribution of congestion-relief benefits. For example, in Sweden where drivers are charged a fee for entering the city of Stockholm, it is estimated that motorists pay $3 in tolls for every $1 of benefit they receive from congestion relief.19 However there are benefits for those who take advantage of increased transit service, and general benefits to the city such as reduced air pollution.
These tradeoffs between equity, environment and financing needs can be addressed by reinvesting net revenues in creating more travel choices in order to yield the highest net public benefits. In this way, cordon pricing not only reflects the true economic cost of drivers’ use of the roadway network, reduces demand on the system, and promotes equitable treatment of disadvantaged groups; it can generate significant revenue to pay for needed projects.
Mr. Chairman, I applaud you and this commission for your willingness to explore these innovative strategies to fund the MTA’s capital and operating needs. Certainly pitfalls exist and implementation will not be easy. And yet the New York metropolitan area cannot afford to have a transit system that is hampered from operating at its fullest and most efficient potential.
Thank you very much for providing me the opportunity to appear before you today.
The views expressed in this testimony are those of the author alone and do not necessarily represent those of the staff, officers, or trustees of The Brookings Institution.
1U.S. Department of Transportation, “Traffic Volume Trends,” Federal Highway Administration, Office of Highway Policy Information, June 2008.
2American Public Transportation Association, “Public Transportation Ridership Statistics,” various years.
3American Public Transportation Association, “Public Transportation Ridership Statistics,” September 2008.
4American Public Transportation Association, “Impact of Rising Fuel Costs on Transit Services: Survey Results,” May 2008.
5See e.g., Janet Rothenberg Pack, “You Ride, I’ll Pay: Societal Benefits and Transit Subsidies,” Brookings Review 10 (3) (1992) pp. 48–52.
6According to the Texas Transportation Institute, the annual cost of traffic congestion in the New York metropolitan area would increase by $4.2 billion if public transportation service were discontinued. David Schrank and Tim Lomax, “The 2007 Annual Urban Mobility Report,” Texas Transportation Institute, Texas A&M University System: 2007.
7The economic benefits of transit are well documented. A 1997 report found that every dollar invested in transit yields six in economic returns. See e.g., Donald H. Camph, “Dollars and Sense: Economic Case for Public Transportation in America,” Los Angeles: The Campaign for Efficient Passenger Transportation, 1997.
8Robert Puentes and Ryan Prince, “Fueling Transportation Finance: A Primer on the Gas Tax,” in Taking the High Road: A Metropolitan Agenda for Transportation Reform, B. Katz and R. Puentes, eds., Brookings, 2005.
9See e.g., U.S. Department of Transportation, “Highway Trust Fund: Fact Sheet,” September 2008.
10Todd Goldman and Martin Wachs, “A Quiet Revolution in Transportation Finance,” Transportation Quarterly 57 (1) (2003) pp. 19–32.
11This proposal faced strong opposition and was eventually scrapped. Officials also shelved a $1 per day region-wide commuter parking tax in 2002. Such a levy was estimated to have generated $1 billion over three years and would have been applied to commercial garages, as well as parking spaces that employees provide for their workers.
12Donald Shoup, The High Cost of Free Parking, American Planning Association: 2005.
13Jeffrey J. Smith and Thomas A. Gihring, “Financing Transit Systems through Value Capture: An Annotated Bibliography,” American Journal of Economics and Sociology, 65 (3) (2006) pp. 751-786.
14Todd Goldman and others, “Local Option Transportation Taxes in the United States (Part One: Issues and Trends)” Institute of Transportation Studies, University of California at Berkeley, UCB-ITS-RR-2001-3, 2001.
15Rod Eddington, Speech to the Commonwealth Club, London, December 1, 2006.
16Peter R. Orszag, “Current and Future Investment in Infrastructure,” Testimony before the Committee on the Budget and the Committee on Transportation and Infrastructure, U.S. House of Representatives, May 8, 2008.
17See: Anthony Downs, Still Stuck in Traffic: Coping with Peak Hour Congestion, Brookings Press, 2004.
18See e.g, Gunnar Söderholm, “The Stockholm Trial: Congestion Charging and Improved Public Transport Aimed at Reducing Traffic Jams and Creating a Better Environment,” presentation at State Summit on Innovative Transportation Funding and Financing, Washington, D.C., June 24-25, 2008; Milan Agency for Mobility and Environment, “Study for the Application of Road Pricing in Milan,” undated.
19David King and others, “The Political Calculus of Congestion Pricing,” Transport Policy 14 (2007) 111–123.


This product was added to our catalog on Wednesday 25 July, 2007.
New Wave T5 Two-Foot High-Output Fluorescent Fixtures
The New Wave T5-24. 2ft fluorescent grow light is a complete light fixture with reflector, ballast, housing and hangers. This high high-output fluorescent lamp is perfect for indoor growing.
95% reflective German aluminum reflector.
Major brand solid state electronic ballasts.
Smart Volt ballasts can run on 120, 208, 240, & 277 volt input power for New Wave 42, 44 & 48 only.
New Wave 22, 24 & 28 are 120 volt only
Light weight white steel housing.
Louvered and slotted for efficient cooling.
Comes complete with 2 chrome mounting hangers.
8 power cord.
Purchase your choice of lamps (tubes) separately.
UL and CULUS Listed
The New Wave T5 High-Output Two-Foot Fluorescent Fixture allows you to select your choice of lamps; high output fluorescent tubes are not included in the fixture price and should be purchased separately.
Purchase high output lamps for this fixture:
T5 Lamp - 24" High Output 24W 3000°K
T5 Lamp - 24" High Output 24W 6500°K


Hello everyone, needing to know from the experienced boaters. I'm looking at getting an 24ft osprey, wondering which is better for the boat in gallons per hour. Gasoline or deisel? Which has less maintence? I was told having gasoline can increase the risk of an explosion on he boat? Kind of confused which to go with now? I'm assuming deisel gets more for the money, right?


You've graduated...now what?
Let’s imagine a scenario where you’ve completed your bootcamp right here at Rocket Academy and now have a unique project portfolio that you’re proud of, how should you navigate through this industry effectively with your new skillset?
As a Software Engineer, a common pain point that you might experience now or in the future is the lack of transparency when it comes to compensation. Can you imagine going through several rounds of interviews and tests just to be lowballed when it comes to the job offer? If only you had known of the company’s average salary before wasting your time going for those pointless interviews.
This is a common problem within the tech industry and here are some steps to take to make sure that you’re being well-compensated!
1. First, ensure that you’re a competent coder
In other words, make sure that you prove your worth. Always find ways to improve on your coding skills and take some time to learn problem-solving skills and how to work well with others. You won’t be surprised to find out that most of the good coders are great communicators as well.
Having a strong foundation in coding concepts and grasp of programming languages give you the ability to pursue projects of your interest. Having a repository of Github projects showcases your passion for software engineering and allows you to level up your coding skills at the same time.
Once you’re ready to job hunt and embark on your journey as a professional software engineer, the next step is to...
2. Do a thorough research on the industry before making your decision
Do your research online and try your best to find out more about the industry before applying for jobs! Having knowledge about the industry will be beneficial to you especially during job interviews.
From front-end to back-end engineering, what does each role entail? Resources and mentorship vary greatly from startups to large tech companies, read up on the pros and cons of each position to find out which culture best suits your needs and skill sets.
Learn more from the experienced engineers by listening to podcasts and joining relevant online tech communities on Reddit or dev.to to deep dive into the world of tech and gain an insider’s view of the industry.
3. Level-up by utilizing platforms to give you an edge over others
For instance, NodeFlair is a platform which aims to help people in the tech industry make smarter career decisions. NodeFlair Salaries was created to give more transparency to tech salaries within the industry. Verified user data is used to help give you reliable information on how much companies are paying its employees.
For starters, check out the image below to find out if you’re currently being compensated fairly.
With verified salary data at your fingertips, you now know what to expect even before going for an interview with a prospective company. However, make sure that you use this knowledge as an added advantage when negotiating, and not as a weapon to threaten your current company for higher pay.
By the way, you can be part of the solution by contributing your salary today. Click here to make a difference. Rest assured, all submitted data will be kept strictly anonymous.
With this guide, becoming a software engineer is as easy as 1, 2, 3! Whether you are a mid-career switcher or just looking for greener pastures, having a clear roadmap helps you to reach your goal more effectively.


text:
With Uganda’s growing middle-class picking interest in pets, a place for their treats has been missing on the shopping menu of Kampala.
Businessman Rajiv Ruparelia’s wife, Naiya, has seen an opportunity and will this weekend open doors to her new business in the dog and other pets’ products.
Ms. Naiya Ruparelia is opening Kaya’s Kitchen in Kampala’s upscale suburb, Kololo, one of the hubs for pet owners. Kaya’s Kitchen will see pet owners find a one-stop shopping centre for their pets’ care.
The Ruparelia family are serial entrepreneurs, and Naiya seeing a gap in the dog care industry is not surprising. There are not statistics pointing to the number of pets in Uganda, however recent years have seen more people buying especially dogs as pets.
Kaya’s Kitchen is a retail outlet for organic, specialty pet food.
The launch will take place along the grand opening of partners Ark Organics shop at the Explorer’s Hub at Plot 4 Lower Kololo Terrace Road.
“Pet parents can stop at the store and shop for pet treats, food, grooming products, pet accessories and toys.”
Do you have a story in your community or an opinion to share with us: Email us at firstname.lastname@example.org

domain: pet


Hayley Welsh’s playful murals pair imaginative creatures with universal messages of uplift and encouragement. Often featuring furry, plump critters that seem to be hybrids of dogs, rabbits, and teddy bears, Welsh’s subjects peer through periscopes atop mechanical fish or aboard paper boats, tug clouds from penny farthing bicycles, and sprout trees as antlers. Occasionally, Basquiat-esque crown motifs appear as well.
Stop breadboarding and soldering – start making immediately! Adafruit’s Circuit Playground is jam-packed with LEDs, sensors, buttons, alligator clip pads and more. Build projects with Circuit Playground in a few minutes with the drag-and-drop MakeCode programming site, learn computer science using the CS Discoveries class on code.org, jump into CircuitPython to learn Python and hardware together, or even use Arduino IDE. Circuit Playground Express is the newest and best Circuit Playground board, with support for MakeCode, CircuitPython, and Arduino. It has a powerful processor, 10 NeoPixels, mini speaker, InfraRed receive and transmit, two buttons, a switch, 14 alligator clip pads, and lots of sensors: capacitive touch, IR proximity, temperature, light, motion and sound. A whole wide world of electronics and coding is waiting for you, and it fits in the palm of your hand.


text:
Dai Ichi hotel is located in Sump town Ryoguku. It is well connected with Toie Odeo Line (Right beside the hotel) and JR Sobu line (4 minutes walk). It is situated at a very convenient location. Akihabara (The electric Town of Tokyo) is just 2 stops on JR line. The best part of this hotel is the impeccable view of Sky tree from the hotel rooms. Staffs are courteous and room size is moderate. Smaller than the size of similar hotels in India but bigger enough (Considering it is in Tokyo). Did not have food at the restaurants in the hotel as it is just one stop from Kinsicho Station where you can get all cousins.
All in all I love this hotel for the view, service, room size and convenient location. Internet is provided, but only through LAN cables and no wifi in rooms. So travelers with ipad, and macbook air, very sorry :(. May be you can try the hotel lobby.
There is no airport limousine bus service from this hotel, but reaching airport is not so tough from this place due to its connectivity with JR and Metro.
- Official Description (provided by the hotel):
- Daiichi Hotel Ryogku is located in the heart of Tokyo, taking only 10 minutes from Asakusa and Tokyo Sky Tree.Accessible to popular sightseeing spots in Tokyo within an hour.(Odaiba,Tokyo Disney Resort and more) We provide free Shutle bus service direct to Tokyo Disney Resort. The hotel is connected to the Ryogoku subway station, and most rooms have a view of the Tokyo Sky Tree tower. Rooms at Hotel Dai-ichi Ryogoku come with free wired internet and a flat-screen TV with satellite channels. ... more less
- Reservation Options:
- TripAdvisor is proud to partner with Booking.com, Agoda, 5viajes2012 S.L., JAPANiCAN, Ctrip TA, Hotels.com, Expedia, Rakuten, Priceline, TripOnline SA, Evoline ltd, HotelQuickly and Cancelon so you can book your Dai-ichi Hotel Ryogoku reservations with confidence. We help millions of travellers each month to find the perfect hotel for both holiday and business trips, always with the best discounts and special offers.
- Also Known As:
- Dai-Ichi Hotel Ryogoku Tokyo, Japan
- Dai Ichi Hotel Sumida

domain: travel


BBC Hoddinott Hall, which opened with a veritable fanfare of concerts on the weekend, promises great things. This extra wing of the Wales Millennium Centre is the much-needed home for the BBC National Orchestra and Chorus of Wales. Its audience capacity of 350 complements ideally St David's Hall, where their large-scale performances will continue. First impressions are of a brilliant clarity of sound and an immediacy that draws the listener right into the music.
If the opening programme was chosen to showcase the quality and versatility of both orchestra and hall, the range was certainly there, yet the combination of Alun Hoddinott (in whose memory the hall is named) Varèse, Holt, Beethoven, Sibelius and Ravel was so disparate as to seem random. Nevertheless, moments of intimacy from pianist Lly^r Williams in Beethoven's Choral Fantasy and Baiba Skride in Sibelius's Violin Concerto were wonderfully memorable. Conductor Thierry Fischer's approach was uniformly thorough while, in the premiere of Simon Holt's St Vitus in the Kettle, he realised the instrumental textures and timbres to scintillating effect.
On the following night, this time under the baton of Jac van Steen, it was further good augury for the partnership of BBC NOW and Holt, their new composer-in-association, that his Troubled Light, the suite premiered at last year's Proms, emerged so lucidly and beguilingly. Preceding the Holt, Henze's Eighth Symphony was delivered with suitably celebratory elan, while the striking opening of Hoddinott's symphonic poem Lizard made it particularly poignant to reflect how, had he lived, he would have relished the challenge of writing for this hall.


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Here we describe a novel diabetic murine model utilizing hairless mice for real-time, non-invasive, monitoring of biofilm wound infections of bioluminescent Pseudomonas aeruginosa. This method can be adapted to evaluate infection of other bacterial species and genetically modified microorganisms, including multi-species biofilms, and test the efficacy of antibiofilm strategies.
The presence of bacteria as structured biofilms in chronic wounds, especially in diabetic patients, is thought to prevent wound healing and resolution. Chronic mouse wounds models have been used to understand the underlying interactions between the microorganisms and the host. The models developed to date rely on the use of haired animals and terminal collection of wound tissue for determination of viable bacteria. While significant insight has been gained with these models, this experimental procedure requires a large number of animals and sampling is time consuming. We have developed a novel murine model that incorporates several optimal innovations to evaluate biofilm progression in chronic wounds: a) it utilizes hairless mice, eliminating the need for hair removal; b) applies pre-formed biofilms to the wounds allowing for the immediate evaluation of persistence and effect of these communities on host; c) monitors biofilm progression by quantifying light production by a genetically engineered bioluminescent strain of Pseudomonas aeruginosa, allowing real-time monitoring of the infection thus reducing the number of animals required per study. In this model, a single full-depth wound is produced on the back of STZ-induced diabetic hairless mice and inoculated with biofilms of the P. aeruginosa bioluminescent strain Xen 41. Light output from the wounds is recorded daily in an in vivo imaging system, allowing for in vivo and in situ rapid biofilm visualization and localization of biofilm bacteria within the wounds. This novel method is flexible as it can be used to study other microorganisms, including genetically engineered species and multi-species biofilms, and may be of special value in testing anti-biofilm strategies including antimicrobial occlusive dressings.
Biofilms are complex communities of microorganisms embedded in a matrix of polymeric substances that have been highlighted as a contributing factor for the poor resolution of chronic wounds1. The study of these highly organized, persistent microbial populations is particularly important for diabetic patients where poor circulation on the limbs and altered peripheral sensory mechanisms lead to undetected lesions2. In the United States, it is estimated that 15% of diabetic patients will develop at least one ulcer during the course of their lives. This translates to an economic expenditure of around 28 billion dollars in treatment3,4, not to mention the immensurable emotional and social burden. Understanding the factors that allow microbial communities to persist in the wound bed and the impact these biofilms have in the healing events is imperative to drive better care for affected patients and propel the development of new treatment approaches. Therefore, the establishment of reproducible and translatable in vivo models for exploring bacterial-host interactions is paramount.
Murine models have been successfully developed to study the impact of biofilms in chronic wounds. These models, however, often utilize haired species and evaluate biofilm clearance by plate counts for viable bacterial cells of excised tissue from sacrificed animals, making them time consuming and costly.
A biophotonic alternative to the end point sampling of animals in evaluating infection was first proposed by Contag et al. (1995)5, who developed a method to capture luminescence from constitutively bioluminescent Salmonella typhimurium to measure antibiotic treatment efficacy. Other studies taking advantage of bioluminescence-emitting bacteria followed. For example, Rochetta et al. (2001)6 validated an infection model to study Escherichia coli thigh infections in mice by measuring luminescence using an intensified charge-coupled device and later, Kadurugamuwa et al. (2003)7 took advantage of the photon emitting properties of an engineered strain of Staphylococcus aureus to investigate the efficacy of several antibiotics in a catheter wound model in mice.
The method characterized here presents a straightforward protocol to induce diabetes in hairless mice, produce and inoculate wounds with pre-formed bioluminescent biofilms of P. aeruginosa, and conduct biophotonic monitoring of the infection using an in vivo imaging system. It offers a direct, rapid, in situ, non-invasive and quantitative process to evaluate biofilms in chronic wounds and in addition, allows for additional analysis such as microscopic imaging of the healing wounds, intermittent blood collection for cytokine measurements, and terminal tissue collection for histology.
Animal experiments were approved by the Institutional Animal Care and Use Committee of Michigan State University.
1. Preparation of Occlusive Dressings and Silicone Spacers
- Cut the transparent occlusive dressing to make squares approximately 1 cm x 1 cm with scissors.
- Cut 10 mm circles on a 0.5 mm thick silicone sheet using a 10 mm biopsy punch. Center a 5 mm biopsy punch in the middle of the 10 mm circle and press firmly to create a hole to form a "donut"-like disc that will be used as a splint.
2. Experimental Animals
- Use 8 week old (22-26 g) male SKH-1 mice from a commercial breeder. Keep mice in standard conditions of 21 °C and a 12 h light-dark cycle with free access to food and water.
- To induce diabetes, inject mice intraperitonially with a 13 mg/ml streptozotocin (STZ) solutionand 25% Glucose (250 µl per/ mouse) on 5 consecutive days.
- Make the STZ solution by diluting 65 µg of STZ in 5 µL of 100 mM Citric Acid, pH 4.5.
- Adjust the injected volume for each mouse to a final mass of 65 mg STZ per 1 kg of mouse body weight. Inject control mice with 100 mM Citric Acid solution pH 4.5 on the same days.
- Confirm hyperglycemia by blood glucose monitoring with a glucometer 14 days after the last STZ injection. Diabetic mice may have polyuria and so their bedding may need to be changed more frequently to eliminate wetness and their weights should be monitored 3 times a week.
- Biofilm preparation
- Grow colony biofilms8 to inoculate the wounds. Two days prior to surgery start an overnight culture of bioluminescent P. aeruginosa Xen 41 in tryptic soy broth (TSB) incubated at 37 °C and shaking at 200 rpm and sterilize polycarbonate membrane filters with 0.2 µm pore size by exposure to UV light in a biological safety hood for 15 min per side.
- One day prior to surgery centrifuge overnight culture at 20,000 x g for 2 min and wash 3 times with 1 mL of Dulbelcco's phosphate buffered saline (DPBS) by pipetting up and down.
- Dilute suspension in DPBS to an absorbance of 0.05 at 600 nm.
- Pipette 10 µL of the diluted culture on each membrane resting on a tryptic soy agar (TSA) plate. After allowing to dry, incubate the membranes at 35 °C for 72 h to grow biofilms transferring to fresh TSA plates every 24 h.
- Standard curve
- Prior to the start of the experiment make a standard curve to correlate bioluminescence and bacterial counts.
- Prepare biofilms as described in 3.1.
- Make serial dilutions of biofilms ranging from ½ to 1/24 by mixing biofilm with DPBS and vortexing until a visually homogeneous solution is produced. Pipette 200µL of the diluted solutions in a black 96-well plate and image with the in vivo imaging system.
- Spread plate dilutions onto TSA plates and incubate at 35 °C for 24 h.
- Count colony forming units (CFU) on plates and create a standard curve to correlate bioluminescence and bacterial counts.
4. Wound Surgery
- Induce general anesthesia using isoflurane in 95% oxygen/5% CO2 (to prevent death from ketoacidosis) at a flow rate of 1 L/min and maintain anesthesia using 1-3% isoflurane. Maintain animals on heat mats during surgery.
- Ensure the deep pedal reflexes of the mouse are suppressed by pinching the foot with tweezers and place the mouse in the prone position.
- Administer meloxicam (0.2 mg/kg) via sub-cutaneous injection (30 μl) for pain management.
- Wipe the skin of the back with 10% povidone-iodine three times and an isopropanol pad.
- Use a sterile 4 mm biopsy punch to outline one circular pattern for the wound on one side of the mouse's midline at the level of the shoulders. Outline the pattern with a permanent marker.
- Use serrated forceps to lift the skin in the middle of the outline and iris scissors to create a full-thickness wound that extends through the subcutaneous tissue including the panniculus carnosus and excise the circular piece of tissue.
- Apply a medical waterproof skin adhesive glue to the skin of the mice and position the silicone splint applying mild pressure. Cover the wound with a transparent occlusive dressing. After surgery, individually cage the animals.
5. Postoperative Management
- Administer meloxicam (0.2 mg/kg) once daily via sub-cutaneous injection for post-operative pain relief for the next 2 days.
- Monitor animals daily for manifestations of pain and weight loss. Diabetic animals need insulin injections when they have lost 15% or more of the body weight.
6. Biofilm Inoculum Preparation and Infection
- Inoculate mice 48 h after surgery as described in the following steps.
- Scrape 72 h biofilms from the membranes using a sterile spatula, place it in a microcentrifuge tube and dilute 1:2 in DPBS. Mix by briefly pipetting up and down.
- Spread plate inoculum onto TSA plates to calculate total CFU. To ensure that counts are accurate, break down the biofilm inoculum further by a series of two 1 min vortexing steps intercalated by a 2 min sonication step at 40 kHz in an ultrasonic cleaner.
- Remove dressing covering the wound and silicone splint and take a micrograph of the wound with a microscope with attached camera using a ruler for reference.
- Cut the tips of 200 µL pipette tips and pipette 10 µL of the biofilm inoculum onto each wound.
- Image mouse using the in vivo imaging system using auto settings: exposure time 5-300 s, with medium binning, 1 f/stop and open filter, and field of view C (12.9 cm x 12.9 cm).
- Cover wound with fresh dressing.
7. Wound Measurement and Imaging
- Evaluate the clinical signs of animals daily9.
- Provide food, water and change cages as needed.
- Check integrity of dressings daily. When the dressing is present, only bioluminescence can be measured due to occlusion of the wound. At day 8, dressings are removed and not replaced allowing measurement of wound closure.
- Weigh animals every other day.
- For all days, place mice individually in an isolation chamber equipped with a HEPA filter and image using the in vivo imaging system daily or every other day until luminescence values fall below background level.
- After day 8, induce general anesthesia and take micrographs of the wound with a microscope with attached camera using a ruler for reference every other day until wounds are healed.
8. Histological Analysis
- Euthanize the mice with a flow of 2 l/min of CO2 in an euthanasia chamber after luminescence falls below background levels and the wounds are completely healed. Confirm death by cervical dislocation as a second method of euthanasia.
- Use iris scissors to create a wide, full excision around and under the wound area (around 1 cm in diameter) and preserve the tissue in 4% paraformaldehyde for histological analysis.
- Other analysis: Cytokine detection
- Collect blood retro-orbitally from animals under anesthesia using a capillary glass tube and transfer it to EDTA-treated tubes.
- Centrifuge blood at 2,000 rpm for 20 min at 4 °C and freeze plasma for cytokine detection.
In developing this new model, we observed many advantages in utilizing hairless SKH-1 over C57BL/6J mice, which we have used in the past. Animals subjected to STZ injections normally experience gradual weight loss with the onset of diabetes; however, in wound healing experiments previously conducted by our laboratories reproducing the model presented by Dunn et al. (2012)9 using C57BL/6J, drastic weight loss was observed (Figure 1). In contrast, when using this wound model with SKH-1 mice a statistically significant lower weight loss was observed (P<0.0001, Mann-Whitney U test). Furthermore, no deaths occurred in the diabetic SKH-1 mice cohort infected with P. aeruginosa Xen 41 biofilms while a 40% mortality rate was observed for C57BL/6J infected mice in previous experiments (Figure 2).
Another advantage to the model presented here is that the experimental procedure for the hair removal step mandatory for C57BL/6J mice is unnecessary for SKH-1 mice. Although in our previous experiments with haired mice special attention was given to minimize irritation to the skin, some damage inevitably occurred (Figure 3). Notably, however, the greatest advantage in utilizing hairless mice in this model is the elimination of the problem of hair re-growth observed in long term wound healing studies. In our experience with C57BL/6J mice, hair re-growth varied from animal to animal but given the long-term nature of the studies, it always occurred and interfered with wound area measurements or dislocated wound splints and/or dressings used to cover infected wounds, potentially resulting in drying of the wound (Figure 4).
In the SKH-1 wound healing model, after diabetes is confirmed, surgery can easily be executed to create a circular full-thickness wound on the back of the animal. The silicone splint is kept in place by a medical waterproof skin adhesive and avoids direct contact from the occlusive dressing with the newly created wound bed (Figure 5).
P. aeruginosa Xen 41 bioluminescent biofilms grown on polycarbonate membranes (Figure 6) are easily and aseptically transferred to a syringe to be prepared for delivery to the wounds and the inoculated mice are monitored daily for clinical signs of infection (Figure 7). For this model, we implemented two distinct phases. In the first phase, after inoculation of the biofilm the wound was surrounded by a splint covered with a transparent occlusive dressing. This results in pus accumulation that occluded the wound. Biofilm-containing wounds were imaged daily with the in vivo imaging system to monitor infection development and assess biofilm evolution (Figure 8 and Figure 9). Bioluminescence, recorded as total flux (p/s), can be correlated with bacterial density using a standard curve (Figure 10).
At day 8, the splint and dressing were removed to allow visualization of wound healing. Bioluminescence subsequently drops due to the loss of the pus surrounding the wound; however, bacteria remained associated with the wound as determined by histology. This approach of removing the dressing to measure wound healing has been utilized in other chronic wounds healing studies (REFs). Wound healing progression can be determined by taking micrographs with a camera attached to a microscope (Figure 11).
Figure 1: Comparative percentage weight loss of SKH-1 and C57BL/6J diabetic mice. Day zero corresponds to weight at the day of last (5th) STZ injection. n = 10 mice for SKH-1 and n = 12 mice for C57BL/6J. Please click here to view a larger version of this figure.
Figure 2: Percent survival rates of SKH-1 and C57BL/6J diabetic mice after P. aeruginosa Xen 41 biofilm application (day 1). n = 5 mice for SKH-1 and n = 10 mice for C57BL/6J. Please click here to view a larger version of this figure.
Figure 3: Skin lacerations in the future wounded area after shaving and using depilatory cream on C57BL/6J mice. (A): day of procedure; (B): 4 days after procedure. Please click here to view a larger version of this figure.
Figure 4: (A) C57BL/6J mouse with a partially removed wound splint. (B) Lifting of the splint revealed a healed wound surrounded by fully re-grown hair. Please click here to view a larger version of this figure.
Figure 5: Surgical procedure for wounding SKH-1 mice. (A) demarcation with biopsy punch; (B) outline of the demarcation; (C) wounding completed; (D) application of medical waterproof skin adhesive; (E) glueing splint; (F) wound covered with occlusive dressing. Please click here to view a larger version of this figure.
Figure 6: Preparation of the biofilm inoculum. (A) 72 h colony biofilms of Pseudomonas aeruginosa Xen 41 grown on polycarbonate membranes; (B) measurement of biofilm using a syringe. Please click here to view a larger version of this figure.
Figure 7: SKH-1 diabetic mouse 6 days after wound was inoculated with the P. aeruginosa Xen 41 biofilm. Please click here to view a larger version of this figure.
Figure 8: Monitoring of biofilm infection by tracking bioluminescence evolution over time in diabetic SKH-1 mice. (A) day of biofilm application; (B) 5 days post-biofilm; (C) 8 days post-biofilm; (D) 12 days post-biofilm; (E) 16 days post-biofilm; (F) 20 days post-biofilm. Please click here to view a larger version of this figure.
Figure 9: Total flux from wounds in SKH-1 diabetic mice infected with P. aeruginosa Xen 41 biofilms during the course of the experiment. Please click here to view a larger version of this figure.
Figure 10: Estimated CFU per wound using a standard curve of bioluminescence per CFU produced with P. aeruginosa Xen 41 biofilms. Please click here to view a larger version of this figure.
Figure 11: Micrograph timeline of wounds infected with P. aeruginosa Xen 41 biofilms in diabetic SKH-1 mouse showing progression of healing. The days after biofilm infection are indicated in the bottom left corner of each picture. Please click here to view a larger version of this figure.
Here we describe a new mouse model for the study of biofilms in diabetic chronic wounds that has many advantages to create a reproducible, translatable, and flexible model.
The first innovation is the use of hairless mice. Other mouse models have been developed to study diabetic chronic wound healing10,11, but all have relied on the use of haired mice requiring the removal of fur by processes that involve either waxing or hair clipping combined with depilatory creams. This step is not only time consuming and messy but potentially injures the skin of the animals in the area where the wound will be placed. While hairless mice have been used in a series of carcinogenesis studies12,13, these mice have not been used to evaluate biofilm persistence in chronic wounds. Another common problem solved by the use of hairless animals, especially in long term studies, is hair re-growth in the wound area, which may jeopardize evaluation of wound healing and disrupt bandaging.
SKH-1 animals also proved amenable to STZ-induced Type I diabetes and, in comparison with C57BL/6J mice, had statistically significant lower weight loss during the course of the experiment, making dosing with insulin unnecessary. This is a particularly interesting trait as treatment with insulin can potentially impact the infection outcome as evidenced by Watters et al. (2014)14 who described an increase in bacterial counts in insulin-treated diabetic animals infected with P. aeruginosa biofilms in comparison to no insulin counterparts. In addition, in our model, there was a drastic reduction in mortality rates in the hairless cohort indicating that the animals are potentially more resilient in dealing with infection.
A second feature of this model is the application of measured pre-formed biofilm inoculum slurry to infect the wounds in contrast to planktonic grown cells. By delivering an already metabolically complex and structured bacterial community to the wound, the bacterial cells are able to evade the immune system and the immediate effects of the biofilms on the lesions can be determined.
The third advantage of this new wound model is the use of a microbial strain capable of producing bioluminescence that can be measured with an in vivo imaging system to spatially localize and quantify the bacteria. This allows real-time tracking of biofilm evolution over time. The P. aeruginosa Xen 41 strain possesses a single stable copy of the P. luminescences luxCDABE operon on the bacterial chromosome that results in the constitutive emission of luminescence, which can be captured by the ultra-sensitive camera in the imaging system. This real-time, non-invasive, in situ feature allows measuring of biofilm by bioluminescence even while the splint and cover are in place. This feature drastically decreases the number of animals needed per study, as there is no need to sacrifice animals at certain time points for biofilm monitoring. However, the presence of biofilm and pus occluded measuring wound healing. In this study, we removed the dressing at day 8 to allow visualization of wound healing, but this parameter could be modified depending on the questions being addressed.
Lastly, as the imaging system is capable of detecting bioluminescence up to 2.5 cm in depth, the newly proposed model is amenable to testing of antimicrobial therapies whether in form of solution or gels or incorporated to occlusive dressings. A real-time infection monitoring model allows much greater flexibility to measure the impact of different dosing concentrations and durations as opposed to a static end-point assay. This model can contribute to the validation of potential novel treatments to eradicate biofilms in chronic wounds.
The authors declare that they have no competing financial interests.
The authors would like to thank the American Diabetes Association for supporting this work (Grant # #7-13-BS-180), the Michigan State University Research Technology Support Facility for providing training and access to the in vivo imaging system and the Michigan State University Investigative Histopathology Lab for processing the mouse biopsies for histopathological examination.
|Opsite||Smith & Nephew||Model 66000041||Smith & Nephew Flexfix Opsite Transparent Adhesive Film Roll 4" x 11yards|
|SKH-1 mice Crl:SKH1-Hrhr||Charles River Breeding Laboratories||SKH1||Hairless mice, 8 weeks old|
|Streptozotocin (STZ)||Sigma Aldrich||S0130-1G||Streptozocin powder, 1g|
|AccuChek glucometer||Accu-Chek Roche||Art No. 05046025001||ACCU-CHEK CompactPlus Diabetes Monitoring Care Kit|
|Pseudomonas aeruginosa Xen 41||Perkin Elmer||119229||Bioluminescent Pseudomonas aeruginosa|
|Polycarbonate membrane filters||Sigma Aldrich||P9199||Millipore polycarbonate membrane filters with 0.2 μm pore size|
|Dulbelcco phosphate buffer saline (DPBS)||Sigma Aldrich||D8537||PBS|
|Tryptic soy agar||Sigma Aldrich||22091||Culture agar|
|Meloxicam||Henry Schein Animal Health||49755||Eloxiject (Meloxicam) 5mg/mL, solution for injection|
|10% povidone-iodine (Betadine)||Purdue Products LP||301879-OA||Swabstick, Betadine Solution. Antiseptic. Individ. Wrapped, 200/case|
|4% paraformaldehyde||Fisher Scientific||AAJ61899AK||Alfa Aesar Paraformaldehyde, 4% in PBS|
|Capillary glass tube||Fisher Scientific||22-362-566||Heparinized Micro-Hematocrit Capillary Tubes|
|Silicone to make splints||Invitrogen Life Technologies Corp||P-18178||Press-to-Seal Silicone Sheet, 13cm x 18cm, 0.5mm thick, set of 5 sheets|
|Tryptic soy broth||Sigma Aldrich||22092||Culture broth|
|IVIS Spectrum||Perkin Elmer||124262||In vivo imaging system|
|IVIS Spectrum Isolation chamber||Perkin Elmer||123997||XIC-3 animal isolation chamber|
|HEPA filter||Teleflex||28022||Gibeck ISO-Gard HEPA Light number 28022|
|Biopsy punches||VWR International Inc||21909-142||Disposable Biopsy Punch, 5mm, Sterile, pack of 50.|
|Biopsy punches||VWR International Inc||21909-140||Disposable Biopsy Punch, 4mm, Sterile, pack of 50.|
|Glucose||J.T.Baker||1916-01||Dextrose, Anhydrous, Powder|
|Citric acid||Sigma Aldrich||C2404-100G||Citric Acid|
|Mastisol||Eloquest Healthcare||HRI 0496-0523-48||Mastisol Medical Liquid Adhesive 2/3 mL vial, box of 48|
|Corning 96-well black plates||Fisher Scientific||07-200-567||96-well clear bottom black polysterene microplates|
|25 gauge 5/8 inch needle||BD||305122||Regular bevel needle|
|Bransonic M Ultrasonic Cleaning Bath||Branson Ultrasonics||N/A||Ultrasonic Cleaner|
- James, G. A., et al. Biofilms in chronic wounds. Wound Repair Regen. 16, (1), 37-44 (2008).
- Gordois, A., Scuffham, P., Shearer, A., Oglesby, A., Tobian, J. A. The health care costs of diabetic peripheral neuropathy in the US. Diabetes Care. 26, (6), 1790-1795 (2003).
- Reiber, G. E., McDonell, M. B., Schleyer, A. M., Fihn, S. D., Reda, D. J. A comprehensive system for quality improvement in ambulatory care: assessing the quality of diabetes care. Patient Educ Couns. 26, (1-3), 337-341 (1995).
- Driver, V. R., Fabbi, M., Lavery, L. A., Gibbons, G. The costs of diabetic foot: The economic case for the limb salvage team. J Vasc Surg. 52, (Suppl 3), 17S-22S (2010).
- Contag, C. H., et al. Photonic detection of bacterial pathogens in living hosts. Mol Microbiol. 18, (4), 593-603 (1995).
- Rocchetta, H. L., et al. Validation of a noninvasive, real-time imaging technology using bioluminescent Escherichia coli in the neutropenic mouse thigh model of infection. Antimicrob Agents Chemother. 45, (1), 129-137 (2001).
- Kadurugamuwa, J. L., et al. Rapid direct method for monitoring antibiotics in a mouse model of bacterial biofilm infection. Antimicrob Agents Chemother. 47, (0066-4804), 3130-3137 (2003).
- Anderl, J. N., Franklin, M. J., Stewart, P. S. Role of antibiotic penetration limitation in Klebsiella pneumoniae biofilm resistance to ampicillin and ciprofloxacin. Antimicrob Agents Chemother. 44, (7), 1818-1824 (2000).
- Morton, D. B. A systematic approach for establishing humane endpoints. ILAR J. 41, (2), 80-86 (2000).
- Dunn, L., et al. Murine model of wound healing. J Vis Exp. (75), e50265 (2013).
- Zhao, G., et al. Delayed wound healing in diabetic (db/db) mice with Pseudomonas aeruginosa biofilm challenge - a model for the study of chronic wounds. Wound Repair Regen. 18, (5), 467-477 (2010).
- Holley, A. K., Xu, Y., Noel, T., Bakthavatchalu, V., Batinic-Haberle, I., St. Clair, D. K. Manganese superoxide dismutase-mediated inside-out signaling in HaCaT human keratinocytes and SKH-1 mouse skin. Antioxid Redox Signal. 20, (15), 2347-2360 (2014).
- Abbas, S., Alam, S., Pal, A., Kumar, M., Singh, D., Ansari, K. M. UVB exposure enhanced benzanthrone-induced inflammatory responses in SKH-1 mouse skin by activating the expression of COX-2 and iNOS through MAP kinases/NF-ĸB/AP-1 signalling pathways. Food Chem Toxicol. 96, 183-190 (2016).
- Watters, C., Everett, J. A., Haley, C., Clinton, A., Rumbaugh, K. P. Insulin treatment modulates the host immune system to enhance Pseudomonas aeruginosa wound biofilms. Infect Immun. 82, (1), 92-100 (2014).

domain: medical


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School: Central Michigan University
Course: United States and Canada
Professor: David meyer
Term: Spring 2019
Tags: geography, natural, resources, infrastructure, super, corridor, Labor, Force, exports, imports, demographics, population, economy, NAFTA, trade, Megalopolis, Urban, Models, central, plains, agriculture, heartland, manufacture, great, lakes, basic, and employment
Name: GEO350 Exam 2 Study Guide
Description: Here is a completed study guide for Professor Meyer's GEO350 Exam 2!


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Department of Parks
Lake Barkley State Resort Park Presents Comedy Dinner Theater Feb. 27 - Marty Pollio
CADIZ, Ky. – Lake Barkley State Resort Park is hosting a dinner theater Feb. 27 that should bring joy and a lot of laughs to its guests.
Marty Pollio is a star of TV and film. He has performed on the “Tonight Show” with Johnny Carson and Jay Leno and has appeared on Jerry Lewis Telethons. He has also performed part of his act in the movie “Punchline” with Tom Hanks and Sally Field and has had recurring parts on many television shows, including “Night Court,” “Empty Nest” and “Blossom.”
The dinner and show is $25 a person for advance reservation until Feb 24. The ticket price will be $30 a person after that date. There will be limited seating for this event so call early. Call 1-800-325-1708 for reservations.
Families and individuals can enjoy this event and the other entertainment and lodging opportunities at the park. Lake Barkley State Resort Park is the largest state resort park in Kentucky and features a lodge, cottages, the Windows on the Water Restaurant, a seasonal campground, 18-hole golf course, fitness center with indoor pool, marina, fishing, tennis courts, hiking trails, gift shop, and recreational and nature programming. The park is located at 3500 State Park Road in Cadiz. From I-24, take the Highway 68 exit west toward Cadiz.
For more information contact Nick Edmonds at firstname.lastname@example.org or call the lodge at 1-800-325-1708
The Kentucky State Park System is composed of 52 state parks plus an interstate park shared with Virginia. The Department of Parks, an agency of the Tourism, Arts and Heritage Cabinet, operates 17 resort parks with lodges -- more than any other state. For more information on Kentucky parks, visit our Web site at http://www.parks.ky.gov

domain: entertainment


CORALVILLE, Iowa (KWWL)– Coralville Police Department executed a search warrant on the Baymont Inns & Suites by Wyndham at 200 6th Street in Coralville.
The search warrant contained information provided by the Stevens Point, Wisconsin, Police Department as a result of their investigation into an Identity Theft, totaling more than $100,000 in losses by several victims. The Stevens Point, Wisconsin, investigation determined that some or all of the property, obtained illegally through identity theft, was at the Baymont Inns & Suites in Coralville.
Dharmesh G Patel, age 30, of Iowa City, Iowa was arrested. He has been charged by the Coralville Police Department with Theft 1st Degree / Possession of Stolen Property (Class C Felony).
A warrant has also been issued for his arrest by the Stevens Point, Wisconsin, Police Department for crimes committed in their jurisdiction. Dharmesh Patel was transported to the Johnson County Jail and is awaiting an initial appearance.


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Rabotai Jewish A Capella
We are one of Israel’s freshest and most exciting A Cappella groups! Having been formed in the summer of 2017, and having started to actively perform and delight audiences from April 2018, we have already performed nationally and internationally. We not only combine beautiful harmonies in our repertoire, but feature high level professional beatbox/vocal percussion to give each song that added kick! We also offer performances with musicians and live bands, as well as purely A Cappella events.
Our services include:
Bar and Bat Mitzvahs entertainment (purely A Cappella if on Shabbat/Festivals, but can offer pre-Shabbat performances with microphones, and musical Havdalah. Packages can include singing at meals, special A Cappella Kabbalat Shabbat and Tefillah options, Oneg Shabbat, as well as beatbox workshops)
Concerts (A Capella and/or with Live Instrumental Band)
Stand alone professional beatbox workshops or part of other events (Can include up to 4 beatboxers from my Premier Group BEATBOX JLM)
Chol Hamoed events
Youth group and school events
Community Shabbat or weekday events (including corporate events and tourist groups)
Intimate family events in your home.
If you have an event that is not listed here, we probably do it, and try to work with your budget, Just ask!
Location: Israel and Internationally
Daniel Kogan, Wedding/Chuppah, Hod Hasharon, Israel
they were really amazing and made our simcha so much better...I give them a 20 out of 10
Sarah Bergman, Bar Mitzvah, Nir Etzion, Israel
We had a spectacular Shabbat Bar Mitzvah with Rabotai. From our initial communication until after the event, Daniel worked with us brilliantly and went out of his way to personalize the Shabbat musical selections. Each member of the group sang with heart and meaning it touched the soul. Their warmth was infectious and the songs were beautiful. There are so many favorite songs that it would be hard to pick one. The yaamod and Hamalach Hagoel was exquisite. Tefilah for the Medina and chayalim was beyond words. The songs during the meals were catchy, touching and definitely made the simcha the beautiful event that it was.
Michele Gluck, Bar Mitzvah, Modiin, Israel - u guys truly are amazing!!!! all the davening,zemirot Shabbat and songs were filled with ruach lively tunes & melodies and it really gave Aitan’s Bar Mitzva that extra special touch....THANK YOU!!
And of course the BEAT BOX workshop was awesome!!
Michele Jonathan & Aitan Gluck


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Abandonment is one of those secret hurts that manifests itself in so many ways such as loneliness, depression, anger, and fear. I know this firsthand because I experienced all of these feelings growing up but at the core of all of it was abandonment. As I matured in my faith I have found three things, why we feel abandonment, how it manifests itself as destructive behaviors, and the solution for it.
The very moment that my mom gave birth to me, she gave me away. I didn’t know at the time, but the moment that I needed my mom the most, she abandoned me. The same recurring message was sent to me again when my mom divorced, and again when she divorced a second time.
I felt as though all of these people, the people most important in my life were leaving me, and why? It was like a message that was being sent to me (my heart) that said, Dear Joseph, I’m leaving you because I don’t love you.
When the people most important in your life are leaving you, it’s hard not to wonder if something is wrong with you. You add on top of that, growing up in a community that is largely very different from you (racially) along with a lot of racism and you could say I was a pretty broken child inside.
How did I cope with all of this? All of the feelings that I had, anger, loneliness, depression, and fear had become a part of me. I started to believe that people really didn’t love me, that I had nothing to offer this world, that I was a failure, and maybe that was the very reason why everyone had left me. I ended up partying a lot trying to get girls to like me, to see something inside of me that I thought my biological mother had missed.
I drank alcohol and I ended up flunking out of my freshman year of college. I had no idea where I was going, who I was, and if anyone really cared about me.
As I look back, I found that I had used those things as an escape, a place to getaway from the reality that I didn’t want to face. For some of you, the place to escape might be drugs, alcohol, prostitution, abuse, pornography, violence, romance novels, or even behaviors such as putting other’s down in order to make yourself feel better.
All of these behaviors often stem from a core issue of abandonment. When the people we love hurt us and then leave us, we begin to feel as though something is wrong with us, that we have nothing to offer. This can’t be farther from the truth, which brings me to my next point, what is the solution?
I tried a lot of things in this world to try and escape the hurts that wounded me for many years. I also knew a lot of people who tried things such as drugs and violence to escape the pain they felt from experiences they were hurt by. But what I can tell you is that all of those things, all of the things that the world has to offer will only provide temporary relief which is why it’s so easy to become addicted to those places of escape.
Since their so temporary and fleeting, there is no way they will ever satisfy and give you the peace that each one of us so desperately desires.
When I finally found God which took me several years to finally give my life to him because I was so afraid, so hurt, so fearful that God might do the same to me. I also had a hard time believing that God’s promises were true when my experiences told me that promises get broken all the time.
I also thought how could a loving God put me through so much pain, how does that show me that he cares about me? Well, I got an answer one night when my girlfriend’s mother called me. Her mother knew I was adopted but didn’t know I was hurt by it. That night she called my girlfriend to tell me to look up a scripture that God wanted me to know.
Never! Can a mother forget her nursing child? Can she feel no love for the child she has borne? But even if that were possible, I would not forget you!
I couldn’t explain it but I was in tears that night. I knew that God was speaking directly to me and It really put me in tears knowing that he really did care about me.
Have you struggled with feelings of abandonment? Share your experiences below.
Am a victim too am all alone throught out my life
What happened? Feel free to message me if you need prayer and support.
This testimony speaks so much volume to my situation man I feel so awesome because I am dealing with accepting the love of others also God loves. I could be doing good then i fall back into Sin which i hate what i fall back into. I grew up with My father so I never had a Mom to teach me Morals and how to be a True women Of God. the Lord called me at a broken place i felt abondon like no one cares always searching for validation within Human race ( Men) I pray i find my self worth and stop being so angry and upset with my self. Your Blogs have really help me in this tough time of falling Short of his Glory
I’m so glad my testimony spoke to you. Its really hard when we lose a parent in our lives whether thats a result of their own choice or not. Based on talking with many people who have lost parents in both categories, its definitely harder for the child when a parent decides to walk out for whatever reason. It makes sense too. The validation you struggle with I hear a lot from people who came from homes where their parent(s) abandoned them in some sense, emotionally or physically walking out.
I would really work on asking God to help you with not only understanding your worth in Him but also experiencing your worth through God which are two very different things. Ask God to give you some scriptures to meditate on when you are ‘feeling unloved’ to help you.
I’m so glad that my blog has helped you! From your experiences, what sin has been hardest for you? I’ll be writing another article soon on how to deal with a ‘broken home’ and a parent or parents who have abandoned their children. Thanks for stopping by!
” Never! Can a mother forget her nursing child? Can she feel no love for the child she has borne? But even if that were possible, I would not forget you! ”
But to me this scripture doesn’t help. Because STILL, WHERE THE *bleep* WAS HE WHEN I NEEDED HIM?!
He is just like my own old man in that sense: He isn’t there to love me or comfort me when I feel sad, but he is there to punish me and be angry with me. So no, he had not forgotten me, but he sure as hell was not there! And if he was, he was cold and distant!
I also feel abandoned by God. When I was 5 years old an older cousin of mine started to sexually abuse me and no one was there to help me to stop the abuse. It went on for many years and I began to blame myself for what was happening to me. You see I have a sister who is a little older than me and this was not happening to her so it must be me right? I am much older now but still suffer from the abuse not physically but mentally. Why would God let this happen to me and where was he when I needed him the most? I still need him today but have never truly felt his presence.
Really sorry to hear that. I think that is the big fundamental question with suffering isn’t it. Why did God let this happen to me if he loved me so much? I don’t think I have the answer to that question but my guess is that, without suffering we never would really need God or know what love is. It’s kind of like fear, without fear we would never have the opportunity to be courageous.
In my walk with God, I’ve only experienced God in what you would call a transcendent way when I was 23. The rest of the time, I’ve found God’s presence more in the stillness and quietness of just resting my mind and heart as best I could during the early morning hours or late at night. Have you tried anything similar? Let me know or I can recommend some other resources to help.
I do think that God was there with you when you were suffering like that and his heart did break. I do hope perhaps that you can feel God’s presence in your life because that will help in your healing. I’ll be praying for you and do feel free to message me at any time, I’m more easily accessible on the healing from God facebook page.
Sending love and prayers,
I was born into a hard life with even harder beliefs , i was born with aspergers and my dad beat the living shit outta me every day for 7 and a half years , My dad was a satanists and a pig of a man . I’ve been knocked unconscious so many times i have terrible memory . as i grew up i’ve been picked on and called retarded and nobody liked me . i tried to convince my mother he was beating me but we ended up staying with him .When i was a kid i hated god , and i still hold anger in my heart even though i shouldn’t . but i can’t help but feel abandoned , i was angry bc we went to church i heard about god and all the wonderful things he does for people and i had none of that , i was left to die and fend for myself. Many times i had the chance to run away but i didn’t , i would look back and see my brothers and i couldn’t leave them to him , if i left he would have killed them . 2 or 3 years later my mother was lost to a cancer unheard of , everyone in my family dies of cancer .i never got the chance to say goodbye , and she died thinking i hated her ,. I love my mother thou i do resent her for a lot of her choices but i loved her. before she died she meet this nice man and ive lived with him ever since but since i’ve been with him i’ve lost my family entirely , i love my brothers and my step dad more than anything in the world .On my side of the family there’s nothing but assholes and entitled pieces of shit . My piece of shit aunt and grandmother have made my life a living hell by always threatening to get dcf and trying to take us from our happy home . so this year we finally just moved away from them and there dead to me , my family isn’t family , there just piece of shit people . Which is even harder because i’m a family man and i’ve given them every chance and they still choose to screw us over. I’ve lost all hope and faith in family i’ve lost hope in a better tomorrow . I’ve been poor my whole damn life and it gets old quick, My step dad actually used to bring in good money for a couple years until obama came into office and introduced obama care and it got so damn expensive that companies everywhere had to lay off tons of people just so they could pay it and keep up with it .And since then we’ve been living check to check poor off our ass .god led us to colorado so we could have a good life and live freely but with the money god gave us to move and rent a house we’ve used almost all of it and are staying with a friend .We have 7 people living in the back of his house in one large sized room . Some mornings i wake up angry at the world , that i have been forsaken ,Even though i know god has kept me alive this far and has protected my house i still feel abandoned , we’ve been here for a month and a half and still nothing , our money’s just wasting away , my dads tried for jobs but everything is so damn expensive up here , unless you work in the military and pull in 6 figures your not getting a house up here . they have a policy that you have to make three times the rent to even start looking at places and god knows we won’t have that money . the only places that are left are either in terrible places we don’t wanna be or not on the map .I don’t know what to do , i prayed to him last night and woke up angry again that with all this money and fortune the lord’s told us well have we dont and again we’re once again poor . i don’t even think i have a chance for collage right now . It’s hard to try and be a better man , it’s even harder to not become your father . I don’t want to be like him . i want to be a dad and raise my own family and raise my kids right .We may be blessed but no matter what it still feels like my family gets the shit end of the stick .I am of the line of Judah and will teach the world to love again but until the day we wait .Im thankful my mother helped me as i grew up so that i act and think and do as normal people , i still have my quirks but it’s nowhere near as bad when i was a kid .this new generation of snowflakes don’t know , every day they complain about spilled milk while im sitting here with the scars of hell in my face , trying constantly for something more , a better purpose .People nowadays are worried about weather they’ll be accepted into yale or some other rich prep schools while i’m worrying whether or not i can finish school .Life ain’t fair and theres jack shit you can do about it , you just have to suck it up and continue for better or for worse i guess .the world meets no man half way .i’ve been told im a fuck up and failure my whole life but one day the lord will give me my chance ,And all i ask is that i have the chance to show the world what i have , To shock the world and to make it better than it is . Man is wolf to man and if there ever was time of such truth then there be any time but now that we may think and act as men .that we may love and accept one another for who we are .
Abandonment has been a huge part of my life experience. My father abandoned me and my brother when he left our mother. I was 7 years old. My father and his side of the family just stopped having any contact with us. All my aunts, uncles, and cousins just disowned us when our father left. Later, when I was in grade 10, my mother kicked me out of home. Another form of abandonment. About a decade after this came what I call the cascade. Every couple of years, a good friend would just up and move away to not be heard from again. This continued until I had no friends left. Might not be abandonment, but it sure feels like it. Now I have only 2 living relatives. Those family members on my fathers side no longer count as family. Now, 1 of my last 2 family members wants nothing to do with me until I give up my beliefs. I can’t lie to save feelings, even if it is family. Feels like abandonment. Top this off with God completely ignoring me, to the point where it becomes neglect. I gave myself to God through Jesus over 43 years ago. In all that time, God has been totally uninvolved in my life. All I have ever gotten from God is silence, absence, broken promises (Bible), and unanswered prayers. I emphasize the Bible because God has never spoken to me, to promise me anything specific. I have never seen, heard, or felt anything from/of God in my life. Total neglect from God. Under these conditions, I can’t help but feel abandoned by God. Thanks
God bless you, by the Holy Spirit, in Jesus’ holy name, Amen.

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New report tracks telematics impact, provides industry-specific benchmarks
20 hours ago
By Bruce W. Smith
Company owners and fleet mangers cringe when one of their heavy-duty diesel pickups comes into the shop on the bed of a rollback with the front bumper rolled up and fenders caved in.
Wrecks are nothing but lost revenue through vehicle downtime, paying the insurance deductible, and the potential bump in annual insurance rates.
Not good for small business or large.
But there’s a bright side to front-end collisions: It’s a great opportunity to add diesel performance upgrades because the insurance company pays for the installation labor and a healthy portion of the parts.
Usually when a company truck is in a big accident the insurance company is Johnny on the spot, working quickly to get the estimate of repairs handled with the auto body shop.
The list of replacement parts to bring the truck back to “pre-accident condition” is usually long and includes aftermarket or “refurbished” parts equivalent to OEM.
“Wrecks like this are typical of what I see every week, “says Warren Spears, the owner of Spears Auto Repair, in Long Beach, Mississippi.
“The insurance adjuster and I go over every inch of the vehicle to see what’s damaged. Then the adjuster will write it up, print out the parts list (with prices) and labor involved to fix it up. The owner pays the deductible and the insurance company covers the rest.”


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A few weeks ago we discussed the 5 reasons why you need a dash cam. In light of the controversy over the Phoenix driver recording and releasing a video of some players of the Ottawa Senators trash talking their coach, here are some tips you should follow to be a responsible dash cam user.
Check your state’s laws regarding how many parties need to consent to being recorded. Would really be a shame to get some stupid fine or lawsuit over an illegal recording.
Regardless of your states laws, make it obvious you are recording the ride, whether it is an obvious camera, a sticker or you mention it. Its the right thing to do, but you don’t need to make a big deal about it.
Remember you are being recorded as well. The dash cam’s purpose is to help you, not hurt you. If you ever need the recording to prove something, make sure you aren’t submitting something that will hurt your case.
Never share any videos of your customers to the internet without their consent. But if you are doing something fun and positive in the car, and want to share, go for it. But think of it more as building your personal brand. My opinion is to have the consent on video so there are no questions about its authenticity. The last thing you want is Uber suspending you for no reason, or someone coming after you after the fact.

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Enterprise Support Grant26 Jun 2020
The Minister for Employment Affairs and Social Protection, Regina Doherty announced that her Department’s Enterprise Support Grant will be made available to assist self-employed recipients who are exiting the Pandemic Unemployment Payment (PUP) scheme with a once-off grant of up to €1,000 to re-start their business.Read This Post
Covid-19 Business Supports
These range of supports from a variety of sources have been put in place to help businesses of different sizes cope with cashflow issues posed by the Covid-19 restrictions, and to assist them in getting opened up again during their appropriate phase.
Covid-19 Restart Grant
The Government has announced a new €250m Restart Grant, which will give direct grant aid to micro and small businesses to help them with the costs associated with reopening and reemploying workers following COVID-19 closures.
Temporary COVID-19 Wage Subsidy Scheme
Today the Government announced new measures to provide financial support to Irish workers affected by the Covid-19 crisis. As part of these measures, Revenue will operate a Temporary Wage Subsidy Scheme. The scheme, enables employees, whose employers are affected by the pandemic, to receive significant supports directly from their employer. The scheme will run for 12 weeks from 26 March 2020. Draft legislation governing the scheme will be published shortly.
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Keith Richards helped Aretha Franklin with her cover of The Rolling Stones’ “Jumpin’ Jack Flash” on 1 condition
- Aretha Franklin covered “Jumpin’ Jack Flash” by The Rolling Stones.
- Keith Richards appeared in its cover music video.
- Both versions of the song appeared in a movie starring Whoopi Goldberg.
Aretha Franklin covered “Jumpin’ Jack Flash” by The Rolling Stones. Afterwards, Keith Richards said he would appear in his music video for the song under one condition. He also revealed what he thought of Franklin as a pianist.
Keith Richard of the Rolling Stones compared Aretha Franklin to Mick Jagger in a way
The book Keith Richards on Keith Richards: Interviews and Encounters includes an interview from 1989. In it, Richards discussed his collaborations with Mick Jagger. “Having worked separately for a few years, we both learned and developed slightly different styles, so we’re like, ‘OK, you do that one,'” he said.
“Mick plays a lot of guitar on this album,” he said. “He’s a good rhythm player. I sing two or three songs. He sings more rhythmically when he plays, much like Aretha Franklin.
RELATED: Mick Jagger once responded to listeners who felt a Rolling Stones song was anti-feminist
Keith Richards appeared in the video for Aretha Franklin’s cover of The Rolling Stones’ ‘Jumpin’ Jack Flash’ since she was playing the piano
Afterwards, Richards discussed Franklin’s talent. “If you can get her to play the piano and sing at the same time, you get that extra kick,” he said. “When I worked with her on the ‘Jumpin’ Jack Flash’ video, I told her I would appear but only if she played the piano and sang.
“She said, ‘No one has asked me to do this in ages’ and then she said she couldn’t fly anywhere because her fear was too great, and I said, ‘ It’s okay, you play the piano and I’ll take the band to Detroit and I’ll do it there, so it was a 50-50 deal,” he said.
RELATED: Marianne Faithfull wanted all the royalties from a song she wrote with The Rolling Stones’ Mick Jagger and Keith Richards
How ‘Jumpin’ Jack Flash’ and the Aretha Franklin cover performed on the US pop charts
The Rolling Stones’ “Jumpin’ Jack Flash” peaked at number three on the Billboard Hot 100, remaining on the chart for 12 weeks. The Rolling Stones put the song on the compilation album Hot Rocks 1964–1971which reached #4 on the Billboard 200. The album lasted 396 weeks on the charts, making it by far the band’s most successful compilation album in the United States.
Franklin’s cover peaked at number 21 on the Billboard Hot 100 and remained on the chart for 11 weeks. The tune appeared on the album Aretha. Notably, Aretha was Franklin’s third album with the same title. The album peaked at number 32 on the Billboard 200 and remained on the charts for 39 weeks. Notably, both versions of the song appeared in the film Jump Jack Flash with Whoopi Goldberg.
The Rolling Stones’ “Jumpin’ Jack Flash” was a hit, and Franklin’s cover kept the song relevant.
RELATED: The Rolling Stones’ Keith Richards Reveals Who Inspired ‘Jumpin’ Jack Flash’


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A friend recently started making memory bears. I had never heard of them and hers are so cute I just had to tell you about them.
She has made these using discarded clothing as well as yardage. She has also successfully used upholstery fabric for some different textures.
If you want to give it a try, here is the pattern she uses. It’s available from Studio Stitch. (If you don’t see it on their website, just phone them.)
If you want to know more, here is her contact information. I refer friends who want a memory bear to her, since making stuffed animals is not in my skill set!
Kristine Rimmey, email@example.com
Next week I’ll tell you about a particularly spectacular memory bear she made from a wedding dress. Please stay tuned!
That’s a lovely idea, and the ones Kristine made are certainly adorable. I’ve seen these bears before, though often they’ve been made from old fur coats with sentimental value. Can’t say I, personally, would want a bear as a family keepsake, but it’s a nice thought for a child.
I didn’t know about making them from fur coats. I’ll have to tell Kristine!
In our family, there’s an unspoken rule that: ‘everybody needs a teddy bear’. This brings to mind when Ma was dying and baby bro brought her a special bear for her stay in ICU…I have some specially selected clothes of hers that I always thought I could make into a memory quilt (ugh to that as a project).
But a teddy bear would be right in line with a family tradition…plus someone else could make it since stuffed animals are also NOT in my skill (or patience) set!!!!
Thanks for the idea – and yes, I remember seeing a few of these on display when I visited Studio Stitch with you!
She’s teaching a class on making them, though I still don’t think I will do it.
I’m with you!
These are indeed very cute!
I thought of your memory quilt post this week when I saw a YouTube video, where someone made small stuffed cat Christmas tree ornaments (from a person’s clothing) as a memory item. I thought that was a great idea, much less complicated than a quilt, and something that can be shared with lots of friends and family members.
Nice idea! I hadn’t heard of that one. Thanks 🙂
They are very cute. A friend of mine also makes them and fabric bears are somehow more special than their traditional cousins
Memory bears are an awesome idea (catching up on your posts in reverse order lol)!
I’m behind on blog reading and everything else—‘‘tis the season 😁

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