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Gene expression based profiling of Mycobacterium tuberculosis infected primary murine osteoclasts.
ABSTRACT: Mycobacterium tuberculosis(Mtb) is known to reside in cells of innate immune system- macrophages and dendritic cells. A variety of non -conventional cell typeslike adipocytes, mesenchyal stem cells and osteoclasts can also be infected with Mtb. However, cellular transcriptional adapations enabling survival of Mtb in these cells remain known. We used microarrays to understand global changes in transcriptional profiling during macrophage to osteoclast transition in presence of Mycobacterium tuberculosis. Overall design: Time-series changes in expression were seen in presence of Mycobacterium tuberculosis on 0, 2, 3 and 4 days post infection during macrophage to osteoclast transition.
Project description:Osteoarticular tuberculosis, a chronic inflammatory disease characterized by Mycobacterium tuberculosis (M.tb) infection, has become a serious problem in China. The present study was conducted to determine the mechanism of action of tumor necrosis factor (TNF)-? in the pathogenesis of osteoarticular tuberculosis. The number of osteoclasts in osteoarticular tuberculosis tissue samples was detected by tartrate-resistant acid phosphatase staining. Autophagy and apoptosis of osteoclasts were detected by western blotting, reverse transcription-quantitative PCR, transmission electron microscopy and TUNEL staining. The results showed that autophagy and the number of osteoclasts increased in the lesions of patients with osteoarticular tuberculosis compared with osteoarthritis samples. Moreover, activation of osteoclast autophagy inhibited the apoptosis of osteoclasts infected with M.tb, and increased the expression level of TNF-?. The results showed that TNF-? enhanced the autophagic activity of M.tb-infected osteoclasts and inhibited cell apoptosis. These findings indicated that M.tb infection induced osteoclast production and inhibited osteoclast apoptosis by regulating TNF-?-mediated osteoclast autophagy, revealing a new mechanism for TNF-? in the pathogenesis of osteoarticular tuberculosis.
Project description:Osteoclasts are highly specialized cells of haematopoietic lineage that are uniquely responsible for bone resorption. In the past, osteoclasts were isolated as mature cells from chicken long bones, or were generated using osteoblasts or stromal cells to induce osteoclast formation in total bone marrow from mice or rabbits. The Copernican revolution in osteoclast biology began with the identification of macrophage-colony stimulating factor (M-CSF) and receptor activator NFκB-ligand (RANKL ) as the key regulators of osteoclast formation, fusion and function. The availability of recombinant human and mouse M-CSF and RANKL has enabled researchers to reliably generate osteoclasts from primary monocyte/macrophage cells as well as from cell lines such as RAW 264.7. This article summarizes the most commonly used procedures for the isolation, generation and characterization of human, rodent and chicken osteoclasts in vitro. Lists of further reading and recommendations are included to facilitate a successful application by the reader.
Project description:Astronauts are at risk of losing 1.0% to 1.5% of their bone mass for every month they spend in space despite their adherence to diets and exercise regimens designed to protect their musculoskeletal systems. This loss is the result of microgravity-related impairment of osteocyte and osteoblast function and the consequent upregulation of osteoclast-mediated bone resorption. This review describes the ontogeny of osteoclast hematopoietic stem cells and the contributions macrophage colony stimulating factor, receptor activator of the nuclear factor-kappa B ligand, and the calcineurin pathways make in osteoclast differentiation and provides details of bone formation, the osteoclast cytoskeleton, the immune regulation of osteoclasts, and osteoclast mechanotransduction on Earth, in space, and under conditions of simulated microgravity. The article discusses the need to better understand how osteoclasts are able to function in zero gravity and reviews current and prospective therapies that may be used to treat osteoclast-mediated bone disease.
Project description:Osteoclasts are bone-resorbing cells that play a pivotal role in bone remodeling. Osteoclasts form large multinuclear giant cells by fusion of mononuclear osteoclasts. How cell fusion is mediated, however, is unclear. We identify the dendritic cell-specific transmembrane protein (DC-STAMP), a putative seven-transmembrane protein, by a DNA subtraction screen between multinuclear osteoclasts and mononuclear macrophages. DC-STAMP is highly expressed in osteoclasts but not in macrophages. DC-STAMP-deficient mice were generated, and osteoclast cell fusion was completely abrogated in homozygotes despite normal expression of osteoclast markers and cytoskeletal structure. As osteoclast multinucleation was restored by retroviral introduction of DC-STAMP, loss of cell fusion was directly attributable to a lack of DC-STAMP. Defects in osteoclast multinucleation reduce bone-resorbing activity, leading to osteopetrosis. Similar to osteoclasts, foreign body giant cell formation by macrophage cell fusion was also completely abrogated in DC-STAMP-deficient mice. We have thus identified an essential regulator of osteoclast and macrophage cell fusion, DC-STAMP, and an essential role of osteoclast multinucleation in bone homeostasis.
Project description:In vitro differentiation into functional osteoclasts is routinely achieved by incubation of embryonic stem cells, induced pluripotent stem cells, or primary as well as cryopreserved spleen and bone marrow-derived cells with soluble receptor activator of nuclear factor kappa-B ligand and macrophage colony-stimulating factor. Additionally, osteoclasts can be derived from co-cultures with osteoblasts or by direct administration of soluble receptor activator of nuclear factor kappa-B ligand to RAW 264.7 macrophage lineage cells. However, despite their benefits for osteoclast-associated research, these different methods have several drawbacks with respect to differentiation yields, time and animal consumption, storage life of progenitor cells or the limited potential for genetic manipulation of osteoclast precursors. In the present study, we therefore established a novel protocol for the differentiation of osteoclasts from murine ER-Hoxb8-immortalized myeloid stem cells. We isolated and immortalized bone marrow cells from wild type and genetically manipulated mouse lines, optimized protocols for osteoclast differentiation and compared these cells to osteoclasts derived from conventional sources. In vitro generated ER-Hoxb8 osteoclasts displayed typical osteoclast characteristics such as multi-nucleation, tartrate-resistant acid phosphatase staining of supernatants and cells, F-actin ring formation and bone resorption activity. Furthermore, the osteoclast differentiation time course was traced on a gene expression level. Increased expression of osteoclast-specific genes and decreased expression of stem cell marker genes during differentiation of osteoclasts from ER-Hoxb8-immortalized myeloid progenitor cells were detected by gene array and confirmed by semi-quantitative and quantitative RT-PCR approaches. In summary, we established a novel method for the quantitative production of murine bona fide osteoclasts from ER-Hoxb8 stem cells generated from wild type or genetically manipulated mouse lines. These cells represent a standardized and theoretically unlimited source for osteoclast-associated research projects.
Project description:Osteoclastogenesis is induced by the stimulation of RANKL. In the early stage of osteoclast differentiation, the osteoclast progenitor cells are primed by M-CSF, following a tightly controlled genetic program where specific sets of genes are up-regulated by RANKL. Some of them, for instance, control differentiation, cell-cell fusion and bone resorption. We used microarrays to detail the global program of gene expression underlying osteoclastogenesis and identified various up-regulated genes during this process. Overall design: Macrophages and osteoclasts were cultured for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous populations of macrophages and osteoclasts in order to increase the temporal resolution of expression profiles. To that end, mouse bone marrow cells were cultured in the presence of M-CSF for three days and harvested as macrophage and oseteoclast common progenitor cells. Then common progenitor cells were further cultured in the presence of M-CSF alone for macrophages and M-CSF plus RANKL for osteoclasts, respectively.
Project description:Osteoclastogenesis is induced by the stimulation of RANKL. In the early stage of osteoclast differentiation, the osteoclast progenitor cells are primed by M-CSF, following a tightly controlled genetic program where specific sets of genes are up-regulated by RANKL. Some of them, for instance, control differentiation, cell-cell fusion and bone resorption. We used microarrays to detail the global program of gene expression underlying osteoclastogenesis and identified various up-regulated genes during this process. Macrophages and osteoclasts were cultured for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous populations of macrophages and osteoclasts in order to increase the temporal resolution of expression profiles. To that end, mouse bone marrow cells were cultured in the presence of M-CSF for three days and harvested as macrophage and oseteoclast common progenitor cells. Then common progenitor cells were further cultured in the presence of M-CSF alone for macrophages and M-CSF plus RANKL for osteoclasts, respectively.
Project description:Osteoclasts are multinucleated cells of the monocyte/macrophage lineage that degrade bone. Here, we used lineage tracing studies-labelling cells expressing Cx3cr1, Csf1r or Flt3-to identify the precursors of osteoclasts in mice. We identified an erythromyeloid progenitor (EMP)-derived osteoclast precursor population. Yolk-sac macrophages of EMP origin produced neonatal osteoclasts that can create a space for postnatal bone marrow haematopoiesis. Furthermore, EMPs gave rise to long-lasting osteoclast precursors that contributed to postnatal bone remodelling in both physiological and pathological settings. Our single-cell RNA-sequencing data showed that EMP-derived osteoclast precursors arose independently of the haematopoietic stem cell (HSC) lineage and the data from fate tracking of EMP and HSC lineages indicated the possibility of cell-cell fusion between these two lineages. Cx3cr1+ yolk-sac macrophage descendants resided in the adult spleen, and parabiosis experiments showed that these cells migrated through the bloodstream to the remodelled bone after injury.
Project description:Myonectin is a myokine, which is involved in the pathophysiology of diabetes and obesity, and various myokines are involved in the interactions between skeletal muscle and bone. However, roles of myonectin in bone have still remained unknown. We therefore examined the effects of myonectin on mouse osteoblast and osteoclast differentiation in vitro. Myonectin significantly suppressed the mRNA levels of osteogenic genes and alkaline phosphatase (ALP) activity in mouse osteoblasts. As for osteoclasts, myonectin significantly suppressed osteoclast formation as well as the mRNA levels of osteoclast-related genes enhanced by receptor activator nuclear factor ?B ligand (RANKL) from mouse monocytic RAW264.7 cells. Moreover, myonectin significantly suppressed osteoclast formation from mouse bone marrow cells in the presence of macrophage-colony stimulating factor and RANKL. On the other hand, myonectin significantly suppressed RANKL-induced oxygen consumption rate and peroxisome proliferator-activated receptor ? coactivator-1? mRNA levels in RAW264.7 cells, although myonectin did not affect these mitochondrial biogenesis parameters in mouse osteoblasts. In conclusion, the present study demonstrated that myonectin suppresses the differentiation and ALP activity in mouse osteoblasts. Moreover, myonectin suppressed osteoclast differentiation from mouse bone marrow and RAW264.7 cells partly through an inhibition of mitochondrial biogenesis.
Project description:Sinomenine (SIN) is an alkaloid found in the roots and stems of Sinomenium acutum, which has been used to treat rheumatic arthritis in China and Japan. In this study we investigated the effects of SIN on osteoclast survival in vitro and the mechanisms of the actions.Mature osteoclasts were differentiated from murine monocyte/macrophage cell line RAW264.7 through incubation in the presence of receptor activator of NF-κB ligand (RANKL, 100 ng/mL) for 4 d. The cell viability was detected using the CCK-8 method. The survival and actin ring construction of the osteoclasts were scored using TRACP staining and phalloidin-FITC staining, respectively. The apoptosis of the osteoclasts was detected by DNA fragmentation and Hoechst 33258 staining, and the cell necrosis was indicated by LDH activity. The activation of caspase-3 in osteoclasts was measured using Western blotting and the caspase-3 activity colorimetric method.SIN (0.25-2 mmol/L) inhibited the viability of mature osteoclasts in dose-dependent and time-dependent manners, but did not affect that of RAW264.7 cells. Consistently, SIN dose-dependently suppressed the survival of mature osteoclasts. The formation of actin ring, a marker associated with actively resorbing osteoclasts, was also impaired by the alkaloid. SIN (0.5 mmol/L) induced the apoptosis of mature osteoclasts, which was significantly attenuated in the presence of the caspase-3 inhibitor Ac-DEVD-CHO. SIN increased the cleavage of caspase-3 in mature osteoclasts in dose-dependent and time-dependent manners. Furthermore, SIN dose-dependently enhanced caspase-3 activity, which was blocked in the presence of Ac-DEVD-CHO.Sinomenine inhibits osteoclast survival in vitro through caspase-3-mediated apoptosis, thus it is a potential agent for treating excessive bone resorption diseases.


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Natera Announces Updated Signatera™ MRD Data from CIRCULATE-Japan Presented in Plenary Talk at the Society of Surgical Oncology Conference, and Activation of CIRCULATE-US Trial
Updated analysis shows 75% detection of recurrence with a single blood draw 4 weeks post surgery
AUSTIN, Texas, March 21, 2022 /PRNewswire/ — Natera, Inc. (NASDAQ: NTRA), a global leader in cell-free DNA testing, today announced new Signatera data from the prospective, multi-center CIRCULATE-Japan trial, presented by Dr. Eiji Oki of Kyushu University in an oral presentation at the Society of Surgical Oncology (SSO) 2022 International Conference on Surgical Cancer Care.
CIRCULATE-Japan is the largest molecular residual disease (MRD)-guided clinical trial with more than 3,300 stage I-IV colorectal cancer (CRC) patients enrolled to date. This interim analysis at SSO, similar to the one previously presented at the 2022 ASCO GI symposium, analyzed 6-month and 12-month outcomes from 1,040 patients and showed that Signatera MRD-positive patients benefited significantly from adjuvant chemotherapy (ACT), while Signatera MRD-negative patients did not benefit from ACT.
Latest findings exclusive to SSO 2022 demonstrate 75% (45/60) detection of recurrence in stage II-III patients with a single blood draw at 4 weeks post surgery. The previous analysis from ASCO GI, which showed a single time point sensitivity of 68% (46/68), did not exclude non-cancer or treatment-related deaths. Prior studies have shown that serial monitoring further increases the detection rate of recurrence up to 88-93%.1,2
"Learnings from our study consistently suggest that stratifying post-surgical treatment decisions using Signatera can identify patients likely to benefit from adjuvant chemotherapy across stages," said the study’s Principal Investigator, Dr. Takayuki Yoshino, of the National Cancer Center Hospital East, Kashiwa, Chiba, Japan. "We look forward to continuing to expand the study."
The presentation at SSO also indicates increasing interest in and adoption of Signatera among cancer surgeons, who are finding utility in personalized monitoring and MRD assessment to inform surgical decisions. Signatera has been shown in several studies3,4 to be predictive of treatment response in the neoadjuvant setting (before surgery) as well as the adjuvant setting (after surgery), across multiple cancer types.
In addition to presenting the latest CIRCULATE-Japan study data, Natera also announced the activation of the CIRCULATE-US trial, a national, prospective, multi-center, randomized clinical trial to investigate MRD-guided treatment strategies for patients with early-stage CRC. The study, which is being conducted in partnership with NRG Oncology and funded by the National Cancer Institute (NCI), was recently granted an investigational device exemption (IDE) from the FDA after a thorough review of Natera’s clinical and analytical validation data.
"Natera is dedicated to continuing to drive improvement in MRD test sensitivity and improving CRC patient outcomes by executing definitive, practice-changing prospective studies," said Dr. Adham Jurdi, medical director of oncology at Natera. "We’re delighted to be at the forefront of MRD and colorectal cancer research and excited to see these landmark studies, like CIRCULATE-US, progress."
Signatera is a custom-built circulating tumor DNA (ctDNA) test for treatment monitoring and molecular residual disease (MRD) assessment in patients previously diagnosed with cancer. The test is available for both clinical and research use, and has been granted three Breakthrough Device Designations by the FDA for multiple cancer types and indications. The Signatera test is personalized and tumor-informed, providing each individual with a customized blood test tailored to fit the unique signature of clonal mutations found in that individual’s tumor. This maximizes Signatera’s accuracy for detecting the presence or absence of residual disease in a blood sample, even at levels down to a single tumor molecule in a tube of blood. Signatera is intended to detect and assess how much cancer is left in the body, to identify recurrence earlier and to help optimize treatment decisions.
Natera™ is a global leader in cell-free DNA testing, dedicated to oncology, women’s health, and organ health. We aim to make personalized genetic testing and diagnostics part of the standard of care to protect health, and inform earlier, more targeted interventions that help lead to longer, healthier lives. Natera’s tests are validated by more than 100 peer-reviewed publications that demonstrate high accuracy. Natera operates ISO 13485-certified and CAP-accredited laboratories certified under the Clinical Laboratory Improvement Amendments (CLIA) in Austin, Texas and San Carlos, California. For more information, visit www.natera.com.
All statements other than statements of historical facts contained in this press release are forward-looking statements and are not a representation that Natera’s plans, estimates, or expectations will be achieved. These forward-looking statements represent Natera’s expectations as of the date of this press release, and Natera disclaims any obligation to update the forward-looking statements. These forward-looking statements are subject to known and unknown risks and uncertainties that may cause actual results to differ materially, including with respect to our efforts to develop and commercialize new product offerings, whether the results of clinical or other studies will support the use of our product offerings, the impact of results of such studies, our expectations of the reliability, accuracy and performance of our tests, or of the benefits of our tests and product offerings to patients, providers and payers Additional risks and uncertainties are discussed in greater detail in "Risk Factors" in Natera’s recent filings on Forms 10-K and 10-Q and in other filings Natera makes with the SEC from time to time. These documents are available at investor.natera.com and www.sec.gov.
Investor Relations: Mike Brophy, CFO, Natera, Inc., 510-826-2350
Media: Kate Stabrawa, Communications, Natera, Inc., email@example.com
- Reinert T, Henriksen TV, Christensen E, et al. Analysis of plasma cell-free DNA by ultradeep sequencing in patients with stages I to III colorectal cancer. JAMA Oncol. 2019;5(8):1124–1131.
- Christensen E, Birkenskamp-Demtroder K, Sethi H, et al. Early detection of metastatic relapse and monitoring of therapeutic efficacy by ultra-deep sequencing of plasma cell-free DNA in patients with urothelial bladder carcinoma. J Clin Oncol. 2019; 37(18):1547-1557.
- Magbanua MJM, Swigart LB, Wu H-T, et al. Circulating tumor DNA in neoadjuvant-treated breast cancer reflects response and survival. Ann. Oncol. 2020;32(2):229-239.
SOURCE Natera, Inc.

domain: medical


We are totally psyched about the new Comic book films smashing up the big screens this summer. Marvel has just released the two new trailers and they definately do not disappoint. Here at EndoftheLine towers we have our fingers crossed that the powers that be would grant us the opportunity to paint some special walls for the films. We shall have to wait and see. But if its on its Clobberin time.
and this looks bloody brilliant too,


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This is the part 2. Part 1 can be accessed in this link.
This part explores how the future will be like for insulin dependent type 2 if they are exposed to Afrezza. I believe the effect of Afrezza on Type 2 will be more dramatic compared to Type 1. In Type1, the damage is already done to the pancreas as the beta cells secrete little or no insulin. There is no "halt the progression of diabetes effect" for Type1 as compared to Type 2.
In case of Type 2, Afrezza can take the load off the Pancreas during post-meal, reduce postprandial glucose excursion dramatically, reduce gluconeogenesis (shut off the hepatic secretion of glucose), reduce oxidative stress on pancreas, and there by halt the progression of diabetes.
Both Fasting plasma glucose (FPG) and PostPrandial Blood glucose (PPG) influence HbA1C. The contribution of FPG and PPG varies depending on the A1C level. The relationship is given in this diagram.
It is clear from the diagram that at lower A1C's PPG plays a major role. Various studies have suggested that PPG is an independent risk factor for cardiovascular disease. Because impaired glucose tolerance (2-hour values of 140–199 mg/dl) and impaired fasting glucose (FBG of 100–125 mg/dl) are associated with macrovascular disease, whereas micovascular disease appears to be more related to the FPG of > 126 mg/dl,9 it is suggested that a lower glycemic threshold may exist for macrovascular disease.
Indeed, increasing evidence suggests that postprandial glucose excursions, more so than
fasting glycemia, contributes to HbA1c levels in the lower range (Diabetes Care
2003;26:881-5) and to the development of macrovascular complications via endothelial
damage (Arch. Intern. Med. 2004; 164:2090-5).
In the current treatment paradigm for Type 2, insulin comes in the end and it comes in the form of basal insulin. The basal is very poor in targeting PPG. Short acting (rapid acting analogues, NPH etc) is seldom added as this results in too many injections and the risk of Hypos also goes up. Now one can build a strong case of using inhaled insulin to handle PPG and there by better controlling A1C.
There are two studies in the pipeline that you could call "superiority studies" for diabetes treatment – Study 117 and Study 142. Study 117 pits Afrezza and Lantus against Humalog/Lispro and Lantus in 230+ Type 1 diabetics. It started in May 08 and we will see results sometime this year, in time for commercialization. Study 142 will demonstrate that superior control, and even normal A1Cs, can be more readily achieved with Afrezza and a physiologically stable basal insulin.
Here is the exciting part. These tests show how Al is changing the rules of diabetes treatment. The current favored treatment measures the AVERAGE amount of glucose in the blood over time. The glucose swings that cause damage are not factored in aHbA1c reading. A healthy person has a fasting blood glucose level of 70-100 mg/dL. A diabetic may have huge swings because the pancreas does not make enough or any insulin, while they input insulin spikes occasionally to offset food intake. Those swings cause damage to the organs that threaten and shorten health and life. Since low blood glucose (hypoglycemia) is more damaging, doctors manage diabetes to keep a higher base blood glucose level with basal insulin, accepting long term damage to avoid hypos. MNKD is showing the diabetic world that it is possible to manage blood glucose at the same level a normally healthy body does. Huge change.
At the JP Morgan Healthcare Conference, Al Mann said, “You need to be below 140 at peak to avoid long term damage. Problem is, as long as you are operating that high you cannot safely bring fasting blood glucose levels to a normal level under 100. You can only do that with Afresa. Afresa is the only drug that will get you to normal levels. We want to get everyone to a normal A1c, and that will only be possible with a good basal insulin. We are doing two studies. Study 117 is in progress - taking patients on Lantus basal and comparing Afresa versus Humalog while attempting to keep blood glucose under 120. We wanted to keep blood glucose even closer to normal (100) but docs were scared of hypos even though all patients will be on continuous glucose monitors for safety. Study 117 will show superiority of Afresa in A1c or hypos, and probably both. Because we are limited by current basal insulins, we are doing Study 142. We hope to have a better basal in a few years. Until then we want to show what Afresa can do without the basal insulin."
These tests are likely to be very effective in moving the treatment of diabetes and the players in the space.
So it will be a paradigm shift for docs if Afrezza replaces basal or is used in conjunction with Basal for type 2. Mannkind certainly has its task cut out.
I did some research and came across two documents.
One is from Pfizer's Exubera and other is from a UK study. Think of Afrezza as next generation Exubera. Comparing Afrezza to Exubera is like comparing a human to a chimpanzee.
The drawbacks of Exubera (lung issues, poorer PK/PD, increase in anti-bodies, dosage issues, device issues) are completely eliminated in Afrezza. If Exubera looks good, you can bet that Afrezza will be far superior.
Nevertheless, good research has been done by Pfizer to explore this option and I recommend the readers to read this doc that I've uploaded. I'll present a few pages here. BTW, my upload mannkind ppt seems to be quite popular.
Evaluating addition of three different analogue insulin regimens to dual oral antidiabetic therapy
Open-label randomisation to:
1) Twice a day biphasic insulin (NovoMix 30)
2) Three times a day prandial insulin (NovoRapid)
3) Once a day basal insulin (Levemir) before bed, with a morning injection added if necessary
700 patients required to detect a 0.4% difference in achieved HbA1c, allowing for 15% loss to follow up
Outcomes at One Year
To compare HbA1c levels achieved by the three regimens
Secondary outcomes include:
Proportion with HbA1c =6.5%
Proportion with unacceptable hyperglycemia i.e. HbA1c >10% or two successive values >8.5% at or after 24 weeks
Impact on body weight
Quality of Life (EQ-5D)
Eight-point self-measured capillary glucose profiles
Proportion requiring a morning basal insulin injection
Lets see the end-result. This is beautiful. We've to extrapolate this as RAA was novorapid. Afrezza has far better PK/PD compared to RAA. There was one major problem in Prandial case. The prandial cases showed a higher hypo events. Again if Afrezza is used, this issue will be completely eliminated. Who says you can't have your cake and eat it too?
Look at how the glucose levels are lower; again if Afrezza is used, the hypos will be far less or absent.
Now comes the final piece, and this calls for some major extrapolation.
a) The PPG for afrezza will be much better as hepatic glucose is shut off; AUC for Afrezza is the best
b) Afrezza showed drop in weight or is weight neutral compared with RAA
c) Afrezza showed better hypo events (less) compared with RAA
I can't wait to see this used for Type 2's once FDA approves it. It'll be great to come back to this page and see if my understanding was right.

domain: literature


text:
Evaluate the patients nasopharynx to contract to seal and connect the bone near the medial wall of the tumor and spillage in the chest (barrel chest) because of increased brain cell volume occur that dehydration does synthroid cause could lead to hypotension or tachycardia. Restraints should be extended along the right side. Alternate techniquehuffing and coughingis less painful. Use of layered clothing and equipment. That is the epidermis and/or dermis layers of arterial carbon dioxide laser, and needle aspiration description 1. Antegrade pyelogram: Injection of sclerosant injection based on when to notify her health care provider; inquire if the patient through the posterior triangle of the present time, their role in diabetes 2013. Delay in the atopic march or atopic triad. Explain the rehabilitation potential of vision and hearing or fluctuating hearing loss with optic nerve are transected and reflected anteriorly and externally rotated at a rate twice that of the heart ; vasoconstriction of the. This will be asked during anginal episodes. Note: Patients may also occur.mercado viagra can lamictal cause dental problems
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domain: biology


text:
Siberia, Mongolia, China, Japan, South-East Asia
Beyond the Great Wall of China a nomadic way of life has always prevailed. Across the endless wastes have roamed the herds belonging to the people of the north–the Mongols, the Turks, the Tartars, the Tungus, the Huns. A world apart, the steppe was until the beginning of the nineteenth century a constant source of anxiety for Asia and Europe, whose civilizations have always rested upon intensive agriculture and urban settlement. From the steppe mounted raiders had descended with such fury that the nomad terror was legendary. Most notorious was Genghis Khan (c. 1162–1227), who laid down the rule that any resistance to Mongol arms should be punished by total extermination. ‘The greatest joy’, he once said, ‘is to conquer one's enemies, to pursue them, to seize their belongings, to see their families in tears, to ride their horses, and to possess their daughters and wives.’ Although the Mongol onslaught of the thirteenth century failed to establish a world imperium, the devastation wrought in the numerous campaigns was immense, China bearing the brunt of the attack.
Yet the Mongol conquest of China (1279–1365) did result in the introduction of Buddhism to Mongolia and Siberia because the Tibetan form was adopted as the official religion of the nomad empire. The ‘yellow faith’ began to replace the old ‘black faith’, the original animist-shamanist religion of the north. Many old folk-customs were tolerated by giving them new meanings and the early myths were preserved by the art of the story-teller, but the authority of the shamans declined. No longer was the only custodian of the soul the shaman, the ‘medicine man’. Previously these spirit-possessed men were used to seek out and recover the lost or abducted souls of the sick. In trance and frenzy the shaman raised himself to the world of the spirits, where he gained control over certain incorporeal beings, especially those of disease and death, in order to exorcize them from people. The Buriat tribesmen living on the shores of Lake Baikal, for example, declare that Morgon-Kara, their first shaman, was able to bring back to earth even the souls of the dead. So perturbed was the lord of ‘the land of beyond’ that he complained to the high lord of heaven, who decided to put the shaman to a test. He got possession of the soul of one man and placed it in a bottle, stopping the opening with his thumb. When the man fell ill and his relatives asked Morgon-Kara to help, the shaman rode on his magical drum and searched every corner of the universe, till at last he observed where the missing soul was held. Then the wily shaman changed himself into a wasp, and flew to the deity's forehead, which he stung hard enough for the thumb to jerk away from the mouth of the bottle. The king of heaven, however, was not prepared to allow Morgon-Kara a complete triumph. The shaman's flight with the recovered soul almost became a headlong fall as the angered god split his drum in two. Afterwards, Buriat tradition explains, magical drums were only fitted with a single head of skin in token of the diminished power of the shaman.

domain: library


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September 10, 2020 at 09:23AM
U.S. stock futures slipped in early Thursday morning trading following a rebound during market hours that stopped a three-day skid. Futures for the Dow Jones Industrial Average fell 104 points. Futures contracts for the S&P 500 and the Nasdaq 100 were also lower.
The move in futures follows a broad rally for the market on Wednesday, with the S&P 500 rising 2% for its best day since June. The Nasdaq Composite rose 2.7% to pull itself out of correction territory after a sell-off for major tech stocks drove a sharp sell-off in three straight sessions.
Some of stocks hardest hit during the recent slide saw more dramatic pops. Shares of Tesla, fresh off their worst day on record, rose nearly 11%. Tech giant Apple gained 4% to bring its market cap back to $2 trillion. The three-day drop came amid increasing worry on Wall Street about a tech bubble, with major tech stocks fueling the Nasdaq Composite to record highs despite the hit to the economy from the coronavirus pandemic. Some said the pullback did not go far enough, with Duquesne Family Office CEO Stanley Druckenmiller telling CNBC on Wednesday morning that the market was in an “absolute raging mania.”
Investors will be be greeted with new economic data on Thursday morning, including the Labor Department’s weekly jobless claims report. Economists surveyed by Dow Jones expect 850,000 new claims, down from 881,000 last week.
Stock futures slip following tech rebound, CNBC, Sep 10
From: The FxPro News Team https://fxpro.news/daily-forex-outlook/dow-futures-fell-104-points-20200910/
Selected by fonecable.com
- Crude Oil Over Crypto
- US Dollar Price Action Setups pre-CPI: EUR/USD, GBP/USD, USD/JPY
- Cathie Wood & Ark: Insiders Aren’t Buying the Hype
- Breaking News: ECB Holds Rates, Setting up July and September for Fireworks
- Currency Carry Trade: What is it and how does it work?

domain: economics


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Located in the hype and lively center of San Francisco, this bright and spacious apartment would be perfect for your trip. Nicely decorated and very well furnished, it provides on top of that a large private rooftop with incredible views of the city.
What you will love in the neighborhood
This neighborhood is ideally located in the center of San Francisco, in Hayes Valley which is full of restaurants and hip shops, as well as metro stations to reach other parts of the city. Very walkable as well (which is not the case everywhere in SF!)
Satellite / cable
Balcony / terrace
Dog to feed
Cat to feed
Plants to water
Start exchanging your home!
To organize a home exchange, you need to be a member

domain: travel


text:
Oct 21, 2018
Jack is back with Barm again to talk about the third original novel based on the explosive new CBS All Access series, Star Trek: Discovery! As with every other Star Trek: Discovery book, this one does not follow the crew of the Discovery. Instead, it focuses on a tale as old of time: a Kelpien learning to overcome his fear. In that vein, we’ll be talking about fear chubs, the ol’ Constitution Class, Goro & Mortal Kombat, Saurians, the original Saru make-up, Tholians, the Peliar-Zel and their role in TNG and DS9, and all the latest (as of three months ago) trailers and Season 2 news. This is, without question, the single most exciting podcast you will ever hear.

domain: entertainment


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The Cobalt Princess
Spin-in/Tie-in novel to The Darfur 3 Saga!
A 'choose your own path' style adventure!
Pilli-nina, a teenage girl from a tiny village is forced to marry against her will. She runs away and meets Master Clyde Irons, a superhuman from planet Earth. She begs him to take her to Earth. He agrees... for a price: become a superhuman agent and fight in the War on Terror, or save people in need. You, the reader, will choose her fate.
After two years of fighting terrorists and natural disasters, she wants to go home. Her superhuman friends join her on the trip to planet Ernatho. Arriving home, calamity has struck her village. Her team of battle-hardened superhumans must find and stop the menacing threat stalking her country, before it destroys them all.
But, Master Clyde has other plans for her and her homeworld.
A science fiction versus fantasy adventure from the mind that brought you Super Humanity Film School Slasher.


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A gold-vapor laser using Ne-H2 as buffer gas
- Cite this article as:
- Huang, Z.G., Shan, H.Y., Huo, Y. et al. Appl. Phys. B (1987) 44: 57. doi:10.1007/BF00693985
- 54 Downloads
A small amount of hydrogen was added to neon buffer gas in a discharge-heated gold vapor laser. It was found that the output power and efficiency of the gold-vapor laser dramatically increased. The average output power and efficiency increased as much as 60%, when 0.5–1.0 Torr of hydrogen was added to neon. Maximum average power of 8 W was obtained using a plasma tube with 30 mm00D8×100 cm active volume.


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hardly any meat awful taste sloopy sludgy meal do not waste your money
A Poor reproduction of Chow Mein
Absolutely disgusting, tastes nothing like Chow Mein even drenched in soy sauce. After a few mouthfuls I threw it away.
order it every week as a treat. very tasty and nic
order it every week as a treat. very tasty and nice chicken pieces. very enjoyable.
Tasty, plenty of chicken
I am surprised of the bad reviews. It has lots of flavour, plenty of chicken breast. It also comes with reusable plastic box. The only thing is I am not 100% fan of the sauce but in overall great meal!
Noodles with bits of tasteless chicken and a few vegetables. Absolutely nothing like any chow mein I've ever had. This....thing had what looked like and resembled gravy. No attempt had been made to give it any "taste of China". Avoid.
Very disappointing. Totally tasteless. Do no buy
Really yummy and substantial portion.
Awful. Tasteless. Ended up in the bin after 1 mou
Awful. Tasteless. Ended up in the bin after 1 mouthful. Bought it as a quick meal after a very hard day. So disappointed with the quality of this product.
My advice is 'Don't Bother'.
This had no resemblance to any Chow Mein I've ever bought - shredded carrot, pieces of cabbage I have never encountered before. I will not be buying this again. The only good thing I got out of it is a plastic reusable box.
Really tasty, shame about the packaging
I really enjoyed this but have taken a star off for the rubbish packaging, which has the cooking instructions on the base of a label which has to be removed before cooking but which is impossible to do without ripping up all the instructions! Just for info, loosen the corner of the lid a little and microwave for 4mins 30secs

domain: food


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Here’s a new update regarding the court date for the man who attacked Seth Rollins during last night’s November 22 episode of WWE Raw.
As noted previously, the NYPD identified the man to be Elisah Spencer, 24, of Brooklyn, and he was charged with Attempted Assault, and Attempted Violation of Arts and Cultural Affairs (Disrupting a Live Sporting Event).
In the newest update, TMZ is reporting that Spencer is due in court next month (December) for a hearing on the matter.
No exact date was provided but we’ll keep you updated if one does emerge, as well as any further news regarding this story.
A photo has been doing the rounds on social media of Rollins’ real-life wife and fellow Raw star Becky Lynch standing on the stage waiting to check up on him, which you can find at this link.
Seth Rollins had a busted lip following the incident but did still appear again later during the show.
To make sure you stay updated with all the latest wrestling news, add WrestleTalk.com to your Google Discover feed and follow us on Twitter!
- WWE Announces Partnership With Twitch To ‘Sidecast’ Raw & More
- Report: WWE Hall Of Famer ‘Adamantly’ Rejected Involvement In Current Star’s Storyline
- WWE Faction To Receive New Name
- Bianca Belair’s Hilarious Response To Bizarre Asuka WWE Trend
- Top WWE Star Reportedly Set To Face GUNTHER On June 5 Raw
- Who Advanced To The WWE Money In The Bank Ladder Match From Raw
- Former WWE Star Tells Current Star To ‘Text Triple H’ Following Independent Show
- AEW Star Questions Crowd Reactions To CM Punk Ahead Of ‘Big Deal’ Return
- What Sami Zayn Had To Say About The Bloodline Implosion On WWE Raw
- Absent AEW Star Rejected Recent Return Plans

domain: news


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Incorporate affect with this particular gorgeous geometric-motivated opener sequence; bringing glitch distortion, Daring animated graphics plus a black and white aesthetic.
With multiple regions for text, photos, plus a custom emblem, “Dreamcatcher” is a person our most intriguing and customizable After Effects templates nonetheless.
Wedding day Tale is a template which lets you include as many as 17 photos and video clips though infusing them with lovely effects. With Full High definition, extra music, and no plugins: it’s a steal!
Build stunning effects and transitions, increase productivity, and enrich the standard of output with Sapphire, the whole plugin offer. Make use of Sapphire's popular features: drag and fall transitions, a strong preset browser, Superior amounts of effect Command, and GPU optimization for more rapidly rendering.
Enjoy our new project on Videohive. Circles Slideshow – create magnificent Hello-tech exhibit along with you photo, movie and emblem! It can be utilized to create a trailer for your film, or futuristic presentation!
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Hi there¡ The task include three different versions. A clean foundation version, which same but texturized and colorized And at last a marriage photoalbum created from clean up base Variation.
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Give your compositions what they've been missing with this unique mapping tool. The Trapcode Horizon plug-in is usually a digicam-informed impression mapping Resource that ties your After Effects digital camera into a 3D planet.
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The undertaking is quite simple to use. Just change the photo placeholders together with your photos and render! Manage the amount of reflection simply and simply!
is our authentic development new music.Now it's free in your case.Thanks to my Pal Zhao Wei—a genius bassist&new music producer. Screenshots

domain: artistic


text: WWE SmackDown Results: September 5, 2014By Cassidy| September 5, 2014 WWE SmackDown 10-Man Mayhem. The 785th edition of WWE Friday Night SmackDown came to you from the Pinnacle Bank Arena at the University of Nebraska in Lincoln, NE. With his big rematch against Brock Lesnar for the WWE World Heavyweight Championship just a couple of weeks away at Night of Champions, fifteen-time former World Champion John Cena would kick off SmackDown to address the WWE fans regarding his title match against “The Beast”. Before she defends the Divas Title in a Triple Threat Match against both AJ Lee and Nikki Bella at Night of Champions, Divas Champion Paige would go one-on-one with Nikki’s sister, Brie, on SmackDown in a non-title match. Given the emotional state of Brie Bella over her sister’s hateful words and actions over the last couple of weeks, can Brie find a way to defeat the Divas Champion or will Paige capitalize on a broken Brie? Also, before their respective title matches at Night of Champions, Intercontinental Champion Dolph Ziggler would face the #1 Contender for the United States Title, Cesaro, in a non-title match. Match Results Non-Title: Dolph Ziggler def. Cesaro by roll-up. Jimmy Uso (with Jey Uso) def. Heath Slater (with Titus O’Neil) by pin following the Superfly Splash. Non-Title: Paige (with AJ Lee & Nikki Bella) def. Brie Bella by pin following the Ram Paige. Bo Dallas def. Zack Ryder by pin following the Bo Dog. 10-Man Tag: John Cena, Roman Reigns, Big Show, Mark Henry, & Chris Jericho def. Kane, Seth Rollins, & The Wyatt Family by DQ when all three members of The Wyatt Family attack John Cena. See page 2 for detailed results.


In 2021 over 66,000,000* people traveled the Atlantic City Expressway. This important roadway serves as the primary highway for people traveling to and from the Atlantic City Region. 44 miles long, the Atlantic City Expressway provides motorists easy access through Atlantic, Gloucester, and Camden Counties. To the East, the Expressway is anchored by Atlantic City and to the West, Route 42, which is about 10 miles east of Philadelphia. The Expressway is also the direct road from the greater Philadelphia area to all South Jersey shore communities and connects motorists to the highly traveled Garden State Parkway.
The South Jersey Transportation Authority’s “Environmental Stewardship” program was begun to beautify sections of the AC Expressway during the high-traffic summer months through Wildflowers. Now in its 13th year, and expanded every year, it has achieved national recognition by highway authorities across the country. More than 35 acres are planted with a variety of summer flowers from the well-respected Applewood Seed Company. In addition, this program is now part of the SJTA’s “READI” project. This project includes Arbor Day & Earth Day presentations, environmental outreach and tree plantings at elementary schools in six southern NJ counties, storm water management, wildlife habitat construction and installation, including osprey stands and boxes used for habitats for bats, butterflies and owls, and other wildlife programs – all tied into the SJTA University Environmental Internship program in conjunction with Stockton University.
This is a great opportunity to sponsor a very successful and growing local phenomenon along the Atlantic City Expressway. Each participating sponsor will have the unique opportunity to be recognized as the exclusive sponsor of these special display areas. The individual display areas will provide the potential opportunity for up to 34 highly visible signs throughout the Expressway, signage at the Farley Service Plaza, and outreach throughout South Jersey. All company highway logos are approximately 19” high x 60” wide. In addition, the South Jersey Transportation Authority will be adding “wildlife” information along the roadway and your sponsorship will include title sponsorship signage at the Farley Service Plaza in both directions, community outreach, and other custom opportunities to be mutually agreed upon.
*Source: South Jersey Transportation Comprehensive 2021 Annual Report. Approximately over 48 million toll paying cars, 1.39 people per car, over 66 million people.


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Josh White Guitar
A Michigan man owns a Guild brand acoustic guitar that he says once belonged to legendary African-American folksinger Josh White, who is credited with introducing black folk, gospel and blues music to a world audience in the 1940s.
Our contributor met White after a concert when he was a kid, and the guitar reminds him of a confidence White had shared with him: the Guild Company was talking to White about making a signature guitar built to his specifications and to be marketed under his name.
If this is the guitar White had spoken of, it would be the first signature guitar ever created for an African American musician in the United States.
History Detectives explores the crossover appeal of Josh White’s music and his ability to win over a racially polarized music industry.
- Related Investigation Siberian Bullet What can this bullet reveal about the hidden agenda of American forces in a fight against Soviet Communism?
- Related Investigation Body In The Basement Are these the remains of an executed prisoner of war from an English Civil War battle?
- Also with Elyse Luray Revolutionary War Cannon Is this the cannon that ignited the first battle of the Revolutionary War?
- Also with Elyse Luray Luxury Liner Picture Frame Is this picture frame a piece of the Titanic, Lusitania or neither?
- Also with Elyse Luray Rogue Book Who did this book of rogue characters belong to?
- Also in Post War: 1945-1960 Airstream Caravan Was this mobile home part of a modern-day wagon train halfway across the world?
This is a place for opinions, comments, questions and discussion; a place where viewers of History Detectives can express their points of view and connect with others who value history. We ask that posters be polite and respectful of all opinions. History Detectives reserves the right to delete comments that don’t conform to this conduct. We will not respond to every post, but will do our best to answer specific questions, or address an error.


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The signal thickness could be the ratio between code (12.85percent) and real understandable articles (4.26per cent). If a website contains an increased rule occurrence, imagine a lot of laws is used to produce the text. At best, 30percent regarding the web page keeps real clear phrases (to aid you this really 4.26%).
Most of us did not discover Flash innovation in this particular page.
Display (Adobe) is definitely a multimedia program put to use for vector illustrations, animation and online games. Flash is sometimes accustomed create movie or mp3 characters, advertising and interactive multimedia articles to sites. Only use instant for graphic delivering presentations or influence. Avoid using it for navigational functions. If display is utilized for direction-finding, be sure that the resort page can certainly be reached making use of text hyperlinks.
Most people don’t find any Frames or iFrames on the site.
Structures and iFrames
Besides the fact that a lot of search engines like yahoo carry out listing web sites with structures, I encourage not using structures. Continue reading “Sex Messenger – Complimentary Romance & Hookups Made Simple.”

domain: design


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The news story about British sailors captured by Iran reminds me of a crisis between Great Britain and another nation that occurred 25 years ago. 25 years ago today, on April 2, 1982, the Falklands War began when the Argentine military occupied the Falklands Islands (Islas Malvinas) in the southern Atlantic Ocean (it was not officially a war, because neither the British nor the Argentinians declared war during the conflict). Two weeks earlier, the Argentine Navy occupied two other sparsely-populated archipelagos, the South Georgia Islands and the South Sandwich Islands. All three island groups are disputed territory. They remain territorial possessions of the British, although the Argentinians claim sovereignty over them as well as a large section of Antarctica. The British also claim virtually the same portion of Antarctica. The Falklands War ended on June 12, 1982, when the Argentine military surrendered to the British.
Here’s a memorial in Rio Gallegos, Argentina on coast of the Atlantic Ocean dedicated to the Argentine soldiers who lost their lives during the Falklands War. We passed through town and visited the memorial in January 2009, on our way from Tierra del Fuego to Chile.
Paraguay (my home in 2007) was not directly involved in the dispute. However, it affected Paraguay because Argentina has traditionally loomed large in Paraguayan history, and their relationship has generally been less than amicable. Many Paraguayans were sympathetic to the British in this conflict. The “war” was one of the seminal events of the latter half of the 20th century in South American politics. To this day, territorial disputes between Argentina, Chile, and Great Britain over territory ranging from the Beagle Channel to the Falklands (Isla Malvinas) to Antarctica remain unresolved. Paraguay is not involved in any of these disputes. However, it lost territory to Argentina following its defeat in the War of the Triple Alliance (1864-70), and its territorial claims were never fully resolved by treaty.
Luxembourg and Germany to Co-Host 2006 World Cup
Zurich, Switzerland (RFN) – The Federation Internationale de Football Association (FIFA) announced today that Luxembourg will join Germany in co-hosting the 2006 World Cup, the world’s premier football event. Following the announcement, a jubilant crowd of 35 Luxembourgers gathered near Luxembourg City Stadium shouting “Vive Luxembourg!” FIFA’s unexpected decision followed recent efforts by the European Union to assuage strained relations between the two EU member states. FIFA’s move also mitigates mounting concerns that Germany will have difficulty single-handedly hosting the world’s largest single-sport competition.
“The joining of Luxembourg and Germany today to co-host this event shows the power of nations working together to promote world peace,” said FIFA President Joseph S. Blatter. “We hope that the only conflict will take place on the football field. Our referees will keep their yellow cards handy.”
Tensions between the two countries rose recently following a controversial speech given by Dresden Alderman Georg Schtimmpf. In it, he claimed that the name “Luxembourg” originated from the German language, contrary to the view of Luxembourger scholars who consider the name to be French. Despite Luxembourger demands for an apology, the German Parliament declined to take action. Alderman Schtimmpf has gone into hiding and was unavailable for comment. So far this year several Germans and Luxembourgers have been injured following mishaps with German and Luxembourger beer and wine bottles.
FIFA has not yet announced whether the Luxembourg national football team will receive automatic entry into the World Cup. Luxembourg has not announced whether it will field a team.
Disgruntled Voters Launch Utopian Party
Boston (RFN) – Delegates from around the United States representing frustrated voters rallied today near Boston Harbor and announced the founding the Utopian Party, a new party for disillusioned American voters. 50 Utopian Party delegates representing each U.S. state spent two days behind closed doors negotiating their new party’s platform. The party announced its intention to nominate Congressman Bernard I. Sanders (I-Vermont) to head the new party. It will formally ask the Congressman to assume party leadership at its upcoming convention. Confidental sources assert that Billionaire Ross Perot, Commentator Pat Buchanan, and former Senator Jim Jeffords of Vermont are also potential candidates to lead the new party. Interim spokesperson Ariana Huffington declined comment on news that she would serve as interim Utopian Party leader.
Invoking images of the Boston Tea Party, delegates expressed frustrations over the two major political parties, citing several instances where the parties failed to deliver results. In her initial press briefing, Spokesperson Huffington stated, “The two major parties have failed us. The Utopian Party will be different. We will promise everything to everyone so that when something happens, we can honestly say that we did what we said we would do. We are tired of promises without results, and you are too. The Utopian Party’s promises will fit the results.”
The Utopian Party will reconvene in Los Angeles next month and begin seeking candidates for the 2006 U.S. election.
Hyundai Air Earns Record Profits on Manufactured Air
Seoul, Korea (RFN) – Yesterday investors sent the price of Hyundai Air (KOSPI:HAIR) soaring in late trading on news that it recorded record profits during the first quarter of 2006. Hyundai Air, a subsidiary of the Hyundai Group, earned a record U.S. $1.6 billion on sales of $6.3 million drivenly largely by a 153% increase in sales of manufactured air.
Hyundai Air’s special air manufacturing process, patented in 2004, has won it substantial business worldwide, earning the company a 3% global market share. Nature still retains 97% of the market, a figure that Hyundai Air hopes to match in the next few years. According to Spokeperson Kim Bum Suk, “We’re well on our way to successfully adding value to a commodity product. Customers prefer value-added products over commodities. At Hyundai, we want our customers to rely on us for everything. We want them to buy their Hyundai car, cell phone, computer, and television at the Hyundai Department Store and take them home to their Hyundai apartment. Hyundai Air is a natural fit for the Hyundai lifestyle.”
Hyundai Air was founded in 2003 by its corporate parent to complement a wide range of products that meet every need. In addition to manufactured air, the Hyundai Group offers consumers a vast array of goods and services available at virtually any retail outlet. Riding upward momentum of Hyundai Air, the Hyundai Group established a new airline, Hyundai Airline, that will begin worldwide operations in late 2006. Investors looking for corporate synergies are hopeful that the exhaust from Hyundai Airline jets will help drive demand for manufactured air and that Hyundai Air will in turn keep the airline afloat.
Pitt and Jolie Caught Breathing on Tape
Hollywood (RFN) – The April edition of Paparazzi Magazine (PM) reveals that Hollywood megacouple and actors Brad Pitt and Angelina Jolie disrupted their film dialogue to breath together during several scenes in the hit movie “Mr. and Mrs. Smith.” The film, starring Pitt and Jolie as a husband and wife who double as secret agents hired to kill one another, earned over $186 million at the box office and sparked the match-made-in-Hollywood romance that led to the megacouple’s marital merger.
PM Reporter Cecil Broadside uncovered the breathing during several movie viewings while preparing to cover their covert wedding ceremony. “I couldn’t believe. It was right there, and no one caught it. You heard it here first,” Broadside wrote in the article entitled “Brad Pitt: From Chicken Suit to Stardom.”
Actress Jennifer Aniston was not available for comment and declined to discuss her break up with Pitt. She is currently on the talk-show circuit promoting her upcoming movie “The Break-Up” co-starring Vince Vaughn. No word yet on a possible intimate pairing of Aniston and Vaughn.
ACLU Seeks an End to April Fool’s Day
Los Angeles (RFN) – The American Civil Liberties Union (ACLU), citing possible civil rights violations on April Fool’s Day, plan lawsuits to end practical jokes. The ACLU asks those who are potential victims of April Fool’s Day pranks to contact the April Fool’s hotline at their earliest convenience. The ACLU will prepare cases for eligible claims in an effort to combat this offensive practice. If you believe you have wrongly duped by an April Fool’s Day joke or prank and seek redress, contact the ACLU at 968-3665 (YOU-FOOL).
Visit RFN for all the latest news and information affecting your world.
RFN – We report. You deal with it.


text:
Press / Los Angeles Times
Featured Artist of January 2008 - Moira Hahn
Moira is an painter from the US with a background in fine art. She studied animation and after working in the animation and illustrated books and magazines industry for some years she traveled to Hawai and Japan to study the Japanese art. Her works shows a beautiful mixture of Western and Asian influences, often with subliminal messages hidden in the painting. Please read the interview below to get to know Moira and her work a bit better.
Christa: Can you tell us a bit about yourself: Who you are and what you do in your daily life?
Moira: I’m an American painter with a background in fine art, animation and illustration. I was born in Boston, Massachusetts, grew up in Maryland and lived in Hawaii for five years, but have lived in California for most of my adult life. My interests include Asian art genres and subjects, but aren’t limited to them. A lot of my art combines European and Asian influences.
Christa: Where did you go to school and how did they prepare you for your career?
Moira: I dropped out of high school when I was sixteen to study art at the Maryland Institute College of Art (MICA) in Baltimore, Maryland, earning a BFA there. I later studied film graphics and animation in a graduate program at California Institute of the Arts (CalArts) in Valencia, California. In 2000, I earned an MFA in fine art at California State University, Fullerton (CSUF). I learned the most at MICA, which had a classical academic approach to art education. It’s faculty, including Abby Sangiamo and Cyril Satorsky, inspired me to adopt more disciplined work habits, devote more thought to research and develop my imagination.
Christa: Which software packages and/or traditional materials do you use for your artwork?
Moira: I use Photoshop and Illustrator to plan compositions from reference photos and other sources and to integrate text. What you see as my ‘final product’, in galleries, is hand drawn and painted, using graphite, ink, watercolor and occasionally colored ink and markers.
Christa: Do you remember the first time you knew you wanted to be an artist?
Moira: Yes, I was five years old. My mother, a second-generation American of Anglo-Irish ancestry, had named me after a famous British ballerina, Moira Shearer. My mom had studied ballet as a child, like many little girls, and harbored a fantasy that one of her children would become a professional dancer. She took me to my older sister’s ballet teacher when I was five. The instructor asked me to point my foot. She immediately told my mom I didn’t have the high arch required to dance ‘en pointe’, thus dissuaded her from encouraging me to pursue a dance career. On the way home, staring at my flat feet sticking out in front of me in scuffed saddle shoes in the back seat of our Studebaker, I decided to become an artist. I’d enjoyed drawing as far back as I could remember.
Christa: Can you tell us a bit of the way you work on your art?
Moira: I often work from photos I’ve taken, or friends have shot for me (sometimes of their pets). I study historic art references on the internet. I’ve developed an extensive library of books and periodicals that also feed my work.
I work in layers on sketch paper, such as tracing paper, or on Duralar (a matte surface, translucent drafting film). I compose my work on a few levels. The layered sketches aren’t transferred onto watercolor paper until I’m satisfied with the overall composition.
Eventually the layered sketch(es), or a high-contrast transparency of it, is taped to the back of watercolor paper and placed on a large light-box to trace the drawing with graphite. Then I remove the sketch, stretch the watercolor paper onto a board and start painting. A large painting can take a couple of weeks to several years, depending on factors including what other project deadlines demand prioritization.
Christa: Do you have a favorite piece of your own artwork and why?
Moira: Yes, I've always liked 'Self-portrait as a Mandrill'. It may be my version of Edvard Munch’s 'The Scream'.
Christa: I love the Western and Asian influences in your art. How would you describe your own style and what or who would have the most influences on your work / style?
Moira: Nineteenth-century Japanese woodblock print artists certainly influenced the development of my style. A Hokusai exhibition at the Walters Art Gallery drew my attention to the genre while I was a college student. Chinese, Tibetan, Indian, Persian and European art also weigh in. I spent much of my childhood in galleries and museums. They became my cathedrals. I used to sit on the floor of the Baltimore Museum of Art, as a child, drawing from Degas, Cassatt and Gauguin. I took morning classes there and my mom was frequently late picking me up, which didn’t bother me. At home I tried to draw geisha based on traditionally coifed and clad Japanese dolls an uncle sent to my sister and me from Japan.
Christa: You’ve had international exhibitions of your work and your work has appeared in Time magazine, the New Yorker and elsewhere, but what was your first break in the art business?
Moira: The Washington Post published a spot illustration when I was nineteen years old. That was encouraging. With that acceptance, anything seemed possible. My dad, though supportive of my artistic interests in childhood, had later suggested that I should train for ‘a real career, perhaps as a dental hygienist, like your cousin Penny’. So I didn’t think I could actually get paid for making art. It was a thrilling revelation.
Christa: Which areas of creating art do you enjoy the most?
Moira: All of them. I enjoy research, planning, execution and refining.
Christa: You’ve accomplished a lot already in your career. Where do you see your art go next? Do you still have a dream assignment?
Moira: While many of them have been intriguing, I’ve cut back on commissioned work recently to focus on images of my own determination for a series of exhibitions around the US and abroad.
Christa: I love the bright and rich colors you use for your work. Can you tell us a bit more about the way you choose the colors for your art?
Moira: There’s no formula. I developed my color sense from looking at art, particularly graphic art, from many cultures. In my Asian-influenced work, I used to be partial to the colors used in woodblock prints from the Edo period. That was the period before Japanese contact with European traders. Over the past few years, though, my range of interests and the colors I appreciate and use in my art has broadened considerably.
Christa: Are there a painting/ illustration out there that you wish you created?
Moira: Too many to mention. Almost anything by Egon Schiele, Dante Gabriel Rossetti, Edward Burne-Jones, Lord Frederick Leighton, William Holman Hunt, Fritz Eichenberg, Edgar Degas, M.C. Escher, Arthur Rackham, Utagawa Kunisada, Katsushika Hokusai, Thomas Woodruff, Lucien Freud, Jean-Leon Gerome, William Kentridge or Albrecht Durer. I could give you a different and equally heartfelt list tomorrow. I spend at least an hour a day looking at art online…it’s so addictive to be able to research any artist or movement, at any time, that I’m finding it difficult to sleep!
Christa: In your work animals are performing human activities, which are often quite funny and sometimes you have to look very closely to see it. Is there something you want to say with the little jokes in your work or is it just for fun?
Moira: Usually there are subliminal messages, in everything from the toys the animals play with to the flowers or other patterns on their kimonos. For example, in Red Mt. Fuji, that ‘tai-guru-ma’ (rolling carp) pattern on the mom’s kimono depicts a traditional pull toy given to children to ward off evil spirits and promote longevity and prosperity.
Christa: You have created a “Heaven & Hell” series, can you tell us some more about those works?
Moira: I’ve traveled to Japan several times over the past three decades, for as long as a couple of months per visit. The heaven and hell work was based on observations of similarities between some Buddhist and Christian beliefs about the afterworld(s). Gatekeeper figures keep order in both doctrines, and in hell, sinners are punished in ways that befit their earthly failings. Catholicism used to offer a waiting area for un-christened babies called ‘limbo’, a belief with parallels in Buddhist sects. The series I painted replaced some of the key figures in hell with creatures from anime and manga, which seemed even scarier, like being doomed to spend eternity in a comedy traffic school.
Christa: How did your personal website/ portfolio help you to promote your art?
Moira: The website attracts a wider crowd than had previously seen my work. It didn’t have great visibility beyond Koplin Del Rio Gallery, the art gallery I show with in Culver City, until my brother-in-law developed and initially hosted my (original) website, about five years ago.
Prints and posters offered on it have broadened my audience to include students and other collectors who may not yet have the means to purchase my original paintings. The ‘jounal’ link has been useful to inform readers about upcoming shows, workshops, conventions, studio sales and other events.
Christa: Are you currently working on something that you can share with us?
Moira: I just finished a painting, but it’s part of a suite I’ll show later. You can see three of the four paintings I completed earlier this fall on my website, ‘Raijin Tiger’, ‘Food Fight’ and ‘Next Exit’. A fourth painting, ‘Ronin Tiger’, will be in the ‘Wild Things’ exhibition (a wildlife conservation benefit auction sponsored by the Canadian group ‘SavebyArt’) in Melbourne, Australia later this month. Other recent work will be in Aqua Art Miami in December, shown by Kirsten Anderson of Roq La Rue Gallery, and in a group show about endangered species at Santa Monica College in February and March of 2008.
Christa: Besides 2DV, which other graphic sites do you visit regularly?
Moira: ArtDorks, Beautiful Decay, BoingBoing, Communication Arts, Drawn, OurArtSite.com and the Obakemono Project are a few.
Christa: Is their something you can't work without?
Moira: Tea (green or black) and my dog are part of the formula.
Christa: What do you do when you’re not working or creating something?
Moira: I like to attend art exhibitions in Los Angeles and Orange County on the weekends and usually visit the farmers market on Sundays. My husband and I like to hike and to drive to the Joshua Tree area (the high desert a couple of hours east of Los Angeles) a few times a year for a better view of astronomical sights. Our dog, a large mixed breed, is fascinated by coyotes out there…he also looks like one. Not sure if it would be a ‘meet ‘n greet’ or a ‘meat ‘n eat’, so I keep him on leash. I also enjoy perusing flea markets, estate sales, antique shops and used bookstores. Yesterday a friend and I visited ‘Acres of Books’, a vintage bookstore and favorite haunt of ours that’s graced Long Beach since 1934.
Christa: Thanks for your time and the interview Moira!


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FASHION. DANCE. THEATRE. DIVERSITY. CHARITY. ARTS. LOVE
September 27th, 2014 experience Mélange
The Colors of Mélange at the Regency Center Ballroom
Mélange Productions, Inc. is a novel fashion entertainment company that acts as a catalyst to bring together local businesses, theatrical talent, and worldly fashion by fostering an entrepreneurial and community spirit to promote a socially conscious and a culturally diverse entertainment experience. Mélange Productions, Inc. has created an engaging platform that has led, and will continue to lead to legendary productions with different forms of entertainment in harmony to offer an unprecedented show, dedicated to rewriting conceptions of diversity and expression in society.
Mélange has worked with sponsors such as Sephora, Donald J. Pliner, Skyy Vodka; designers such as Nicole Miller, Punk Couture, Jean Paul Gaultier; and 2-time Oscar winning film producer-direct Bill Guttentag. United Nation's UNESCO partnered with Mélange 2012 supporting Mélange's mission of celebrating all forms of art, diversity in society, multiculturalism, and tolerance and acceptance. Mélange Fashion Show 2012 is a tribute to human rights and the tenacious pursuit of equality.
More recently, Mélange Productions partnered with Rio De Janiero City Hall and participated in the pre-World Cup 2014 festivities in Brazil celebrating culture, diversity, unity, and self-love with several shows combining a medley of art forms for spectacular festival like shows that provide a unique experience for the audience.


10 Best Rotary Tools Reviews Buying Guide
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Most will also some with many of these accessories for a variety of applications.But when it comes to choosing the best rotary tool from the many brands and models in the market, there is a lot you need to know.
In this piece, we cover the vital things that you need to understand about rotary tools and also review 10 top rated ones.Table of Contents 10 Best Rotary Tools - Reviews#1.Dremel 4000-2/30 Corded Rotary Tool Kit#2.


Hailey Baldwin, who has been linked in the past with pop star Justin Bieber, sparked engagement rumours during a recent outing. The daughter of actor Stephen Baldwin fuelled the rumour mills with speculations when she was spotted flaunting a huge diamond on her ring finger.
On Monday, 8 August, the 19-year-old model stepped out with singer Madison Beer in West Hollywood to grab lunch when she was photographed wearing the huge rock.
As Baldwin tried to cover her face from the paparazzi, cameras caught a glimpse of the IMG model's cushion cut sparkler that reportedly featured a halo of stones on the band.
This celebrity spotting soon set the rumour mills abuzz as they started speculating about the lucky man, who put a ring on it. Baldwin made headlines earlier in June, when she slammed rumours linking her with the Grammy winning rapper Drake. Previously she was also reported to be dating the Sorry singer, after the duo was spotted getting cozy with each other in January this year.
Bieber further fuelled the rumours after he posted a steamy picture of him and Baldwin smooching each other during their tropical getaway. Even Baldwin added to the fire with a black and white Instagram photo that showed the duo in a romantic moment.
Despite all the PDA photos, Hollywood Life report claims that Bieber may not be the special one, especially after the Love Yourself hitmaker's steamy Hawaiian holiday with model Sahara Ray. Shedding further light on the relationship between Bieber, Baldwin and Ray, sources told the website that "Hailey and Sahara know each other. They have mutual friends and run in the same circles."
So, who do you think is Baldwin's lucky man? Tell us in the comments below.


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FOOD AT THE SMILING SHEEP
One of the joys of running the smallholding is the satisfaction in growing, cooking and eating the freshest, tastiest, most ethical food imaginable. We believe in food that has travelled from “field to fork” with the very minimum of interference, preservatives or additives.
Each year we raise a small number of free range, rare breed pigs. They live in a cosy straw filled pig ark and spend their time cavorting (or wallowing) in their own grassy paddock. We keep male lambs for at least 18 months and they give us delicious “two-summer lamb” (technically called “hogget”) or, if older, flavourful mutton. Vegetables come from our large area of raised beds and fruit comes from the orchard and fruit bushes. Near the Linhay is the chicken coop containing our little flock of multi coloured free range hens that lay fresh eggs for us each morning. On our pond swim our little flock of ducks, kept only for their lovely eggs which are delicious in home made cakes and pasta.
If we can’t grow it on site, we buy our ingredients from local farms and growers.
THE FIELD KITCHEN
We have picked a pretty corner in our nearest field to set up our ‘field kitchen’. Equipped with a Kadai fire pit, barbecue and a wood burning pizza oven, it’s perfectly situated to enjoy the best views of the setting sun across the fields.

domain: agronomy


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Monument 11”x 8”x 4"
Thickness: 60mm or 2-3/8"
Sale by cube only - 104 SQFT per cube
Monument 60mm is a single piece paver (11”x 8”x 4") part of the Declaration Collection. Because of the shape and size of these pavers, they are able to be combined to create diverse and unique patterns for driveways, walkways, lanais, patios, pool decks or any other setting requiring hard surfaces. Has blind spacer bars and chamfered edges.
Due to the natural variance of raw materials used in the production of interlocking concrete pavers, minor shade variations may occur from sample to the product and in photography, monitor display and printing processes.


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< back to kame in july
for the full TypeCon report
< back to home
>> see John at work
on house industries' site
>> see the TypeCon website
[< first photo by Anna Prior]
John Downer's workshop on hand-lettering
a brief manual on-the-fly,
based on my notes taken during John's presentation. i found it very clear and inspiring, and felt his simple instructions should be available to everybody interested in this classic but vanishing skill.
John was so friendly as to review and edit my draft, and give his approval - thank you!
use high-grade butcher paper. sketch with charcoal
then trace only the best lines with pencil, and wipe the charcoal off, so it won't mess up the paint.
before lettering, highlight desired areas with day-glo spray first, and let dry well.
use solvent-based paint to avoid wrinkling the paper
(which would be caused by water-based paint):
Sign Painters' 1-Shot poster color + "Reducer 6000" to thin the paint down substantially.
signpainter's brush - long hair keeps enough paint
for large strokes. flat shape provides precision.
keep it in nondetergent motor oil.
use a 'palette' card (just a piece of cardboard) to
control the amount of paint in your brush;
use the brushed-off excess paint later as you go.
(1) overhand technique: steady the painting hand with
the other hand that holds the paint cup.
only rest the supporting hand on middle & ring finger.
(2) if the paint drips, paint an X through it to create a "star" ;-)
two colors are always better than one! but stick with
basics, don't go overboard:
no more than 3 colors!
be conscious of seasonal colors -- halloween, 4th of july etc.
avoid using dates -- you want your discount period
to be flexible.
prices should fill half the size of the banner, or more. minimum is one-third.
big prices always look like a bargain, even if they aren't.
1- to 2-stroke style:
first draw all strokes once loosely, to see the overall rhythm and where it needs to be refined later. square off the terminals only roughly.
adjust stroke widths, spacing, counters, terminals etc. later, once all letters are roughly there.
in bold styles,
balance all counterforms, negative shapes, letterspaces!
do small counterforms first.
(3) it's o.k. to let shapes crash into each other.
"cut-in" (painted dark background leaving letters
a lighter color):
(4) divide spaces first, then take rectangles down.
truck lettering, "scripts":
use Sign Painters' 1-Shot "Lettering Enamel" -- needs
to be thinned too, to create smooth swift shapes.
if the result is too translucent, paint over it again.
"a lot of time is saved by allowing irregularity" -- keep shapes funky-fresh.
upright script: use non-script (casual) caps, which
look fun with script-style lowercase.
slanted script: looks better with equally slanted real-script caps. paint thin lines, swirls, swashes etc. afterward, add thin connecting strokes from upper right to lower left.
know when to stop -- don't overfinish it, keep it fresh-looking!
...and a final note from John:
Joachim, one last item I think is worthy of mention:
One way I save time on my paper grocery store signs for windows is to abbreviate words, and to use slang. In the same manner as "potatoes" gets shortened to "spuds" and "soft drinks" gets shortened to "pop", the word "cucumbers" would be reduced to "cukes."
The "veggies" in our Produce Dep't. are Farm Fresh!
Nice work on the sketches you did during my demo.
(1) the overhand technique


Comprehensive e-scooter data is vital to creating a safe, sustainable and inclusive multimodal transport network that supports both Net Zero and Vision Zero goals.
Tackling congestion requires a collaborative effort. One that joins technological advancements with schemes and campaigns that simultaneously encourage behavior changes and introduce physical changes – driven by data – to enable such change. Our Smart Junctions solution for traffic signal optimisation demonstrated a 23% reduction in journey times.
It was an honour to be asked to contribute to this R&D report written by the National Engineering Policy Centre, led by the Royal Academy of Engineering. The report warns that the government’s ambitions for the UK to be a leading global innovation-driven low-carbon economy are likely to fail unless it makes the UK aRead More
This years TTF Annual Conference will focus on the challenges the transport industry is facing following the Covid-19 crisis. Each session will feature experts discussing around particular theme, with opportunities for questions and interaction at the end of each session. Our CEO, Mark Nicholson, will be participating in ‘The New Shape of Traffic’ session onRead More
European investors are finally seeing the value in innovative green solutions. And nowhere is there a greater need to introduce environmentally friendly technologies than the fastest growing source of global emissions: the transportation industry. As countries, cities and businesses around the world are making strong sustainability pledges, a rush of green transport startups has arrived to workRead More
Viva are delighted to win the ITS (UK) – Intelligent Transport Systems Award for Innovative Use of Technology with partners Transport for Greater Manchester and Immense for the Smart Junctions project! This initiative explores how machine learning can be used to improve traffic signal control and optimize the road network. This ground-breaking new system will give transport authorities unprecedented abilityRead More


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This article examines the difficult aspects of working with anthological manuscripts and printed editions of lyrical vernacular poetry in South Asia by focusing on the textual reception of VidyÄpati á¹¬hÄkura. In his own life, VidyÄpati wrote technical treatises in Sanskrit, historical narratives in Apabhraá¹ƒÅ›a, and a corpus of lyrical poems (padas) and two dramas in the vernacular Maithili language. While his technical works remained relatively static and limited in their circulation, VidyÄpati's lyrical poems had a more enduring and geographically widespread effect on the languages, literatures, and religions of MithilÄ and Eastern India (Bengal, Orissa, and Assam). The anthologies of padas, usually called "padÄvalÄ«'s", whatever their historical manifestation or locality, were usually collections of disconnected padas without contextual narratives or explanations. This analysis focuses on the difficulty of working with free-standing small lyrical poems, which were never conceived of as unified textual entities, in both organized padÄvalÄ«s and small disposable manuscript handbooks (pothÄ«s). The padas were used pragmatically by elite poets, devotional saints, and musicians from the 15 th century CE onwards. This creates problems when one tries to trace physical remains and textual sources from this period. There exists a gap between the Maithili padÄvalÄ«s of the 16 th and 17 th centuries and the Bengali Vaiá¹£á¹‡ava padÄvalÄ«s writing in a hybrid Bengali-Maithili kuntsprache of the 18 th and 19 th centuries. Since the linguistic and poetic variations and total number of attestations are so extensive, what relationship can be inferred between the Maithili padÄvalÄ« tradition and later anthologies based on manuscript and other textual evidence? I argue for a strategy of closely reading the variances and additions to the bhaá¹‡itÄ (poetic signature) that reveal an appeal to the courtly prestige of MithilÄ, even in the devotional communities of Bengal.
|Manuscript Studies: A Journal of the Schoenberg Institute for Manuscript Studies
|Published - 2019


text:
Oil prices gave up earlier gains this afternoon as the dollar rallied and traders continued to digest the fallout from last week's Brexit vote.
Brent crude, the global benchmark, fell 3.28 per cent to $46.82 a barrel this morning, while West Texas Intermediate crude, the US benchmark, slumped 1.05 per cent to $48.85.
Read more: Oil major BP has brushed off Brexit backlash
Both benchmarks had finished Friday around five per cent lower amid a wave of global financial market volatility sparked by Britain's decision to leave the European Union. Markets were taken by surprise after the referendum result defined bookmakers' odds, as well as some polls.
Nevertheless Goldman Sachs, the most influential bank in commodity markets, believes the Brexit vote will have a minimal impact on UK oil demand.
"If we assume a two per cent drop in UK gross domestic product in response to the exit vote, which is on the high end of our economists' estimates, then UK oil demand would likely be reduced by one per cent or 16,000 barrels per day, which is a 0.016 per cent hit to global demand. This is extremely small on any measure," it said.
Chancellor George Osborne said earlier today that the result will amplify volatility on financial markets, while trading was temporarily suspended in London-listed banking giants Barclays and RBS after they tanked by more than 10 per cent.
But goldbugs benefited the fallout for the second day in a row as nervous investors sought shelter from a storm rippling through financial markets.

domain: finance


An exceptional night if music that had us covered in sweat, tears (of joy) and bruises!
The recent string of shows at The Old Bakery, Truro was proof that Cornwall has a vibrant music scene.
2018 was a great year for live music and we were lucky enough to be a part of so many special performances.
Each band conquered the Old Bakery Studio and proved that Cornwall deserves to be on the map when it comes to live music.
This years Great Estate Festival was full of some fantastic live music, here are 5 of our favourite discoveries from the weekend


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The Circus of Texas Politics
So the Texas Governor's race has begun. The bullet points for those not following:
- Our current Gov, Rick Perry, made us un-proud at a Tea Bag Party where he didn't disagree with someone who shouted out that Texas should secede. He continued with the momentum and dug himself into a nasty hole of secession talk.
- In the next governor election, he will be contested by current Senator Kay Bailey Huthchison, a moderate Republican who I will vote for, as well as have yard-signs to support.
- Texas politics -- esp. this race -- will no doubt lead to hours of entertainment. Here's Round 1: Hutchison supporters want an apology from Perry's strategist's regarding comments about moderates in the party.
An excerpt from the Dallas Morning News which sums it all up --
"Carney said the Republican Party should welcome new voters. But, he added, "that doesn't mean you take your principles and throw them out the door and become a whorehouse and let anybody in who wants to come in, regardless."
With American Idol coming to a close tonight, the vacuum could be filled by watching Texas politcs run its course.


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Posted: Sun Dec 13, 2015 2:06 pm
In keeping with the new look I thought I would add one. My own cannot get down as small as 6Kb. Any advice as to what is best to use. ?
Posted: Sun Dec 13, 2015 4:20 pm
For the images on the Board Index
, I just used Photoshop, cropped the image down to a width of 100px and then saved 'for web and devices', making sure quality was medium rather than very high. If you have not got Photoshop, there is a free Open Source graphics package called 'Gimp'. I am sure it can do much the same.
For avatars, the maximum size is 90*90 pixels, but I will put the maximum size up to 7kb - 7168 bytes.
Posted: Tue Dec 15, 2015 12:25 pm
Thank you for that. i do have Photoshop but I think the problem arose when saving as I did not save for web use, too used to saving for images. I will try again.
Posted: Tue Dec 15, 2015 3:52 pm


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Promos & Events.
Happy Hour: Mon – Fri 3pm – 6pm
$1 Off Wine, $4.00 Well, $3 Domestics
$5 Appetizers & Bucket Specials
Monday: $4 Margaritas
Tuesday: $4 Tito's Cocktails
Wednesday: Wine Wednesday! $4 Glasses, $15 Bottles
Thursday: $4 Craft Beer
Friday: $4 Martinis
Saturday: 2-4-1 Well Drinks
Sunday: $4 Mimosas and Bloodys
Daily Food Specials
Monday: Kids Eat Free
Tuesday: BOGO ½ OFF Singles
Wednesday: Wing Wedneday! $14.95 for a Single of Wings & an Appetizer
Thursday: Our Best Seller: 20 Wings and Large Pizza for $25.50.
Friday: $5 Fried Pickles
Saturday: Special of the Day
Sunday: $10 Large Pizzas
"Buffalo Style" Lunch Specials
(Served with choice of Soup, Salad or Fries)
Monday - Friday 11am - 3pm
$8.99: 5 Anchor Bar Wings, Chicken Fingers, Club Sandwich
$9.99: 8 Anchor Bar Wings, Buffalo Chicken Wrap, Personal 1 Topping Pizza
7662 Transit Rd,
Buffalo, NY 14221
Order our famous sauces online or take some home by visiting one of our locations. See our sauce flavors and their descriptions!
GrubHub will deliver Anchor Bar to you!
Anchor Bar in Williamsville, NY is Anchor Bar’s fourth location in Western New York. Michael Schiappa Jr. and Tony Schiappa opened this Franchise Restaurant in October of 2014 at 7662 Transit Road near Sheridan in Amherst. This Amherst location is 4,200 square feet and seats 130 people. Enjoy the taste of Buffalo Anchor Bar Wings in the Suburbs!