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SHE’S 92, as fit as a fiddle and as smart as a whip snake. She’s funny, sassy, and on the ball. Except for one thing. She’s blind from macular degeneration and she sports a broken heart. Not much she can do about either. Except carry on.
I met Mrs D this week during the course of my job. Sometimes as journalist, when you wade through the quagmire of crap that’s delivered to your lap, and peer beyond the press releases and pitches, you find a gem. Mrs D was pure gold.
We weren’t meant to meet. Or maybe we were. It was a simple phone interview to talk about her school reunion, draping herself in the old school tie, or ties as they may be, after 75 years. But once it became apparent she could not see, was not internet savvy, and had no way of sending me photos of herself, I was given the privilege of visiting her at her home.
She greeted me with a python-like hug. “I’m so glad to meet you,” she said, not quite looking at me, as she couldn’t see me, just perhaps maybe my form. She looked 62, not 92. And she sported a wicked wit. She spoke with great pride of her grandchildren, one in Paris, another in Antarctica. The one in Antarctica has a girlfriend, Jessica, working as surveyor on a mine in Queensland’s Cloncurry. Mrs D wasn’t quite sure what to make of Jessica at first. “I wondered what kind of girl she would be, but then she turned up and she was tinier than me. I fell in love with her.” So much so, that Mrs D declined to comment on the fact her grandson and Jessica were “living in sin” in case “they didn’t talk to me anymore”. I couldn’t imagine that ever happening with this pocket rocket. Mrs D so loves Jessica, she ensures that each time she’s in Brisbane, even without the grandson who is still in Antarctica, she plans a family dinner. “She’s such a lovely girl, we want to keep her in our family,” Mrs D said.
I told Mrs D that she looked superb for 92. “That’s the good thing about not being able to see,” she said, “you can’t see all your wrinkles,” she smiled, nimble fingers stroking her face.
But among the bunches of lavender on her table and the crocheted doilies, this wasn’t what struck me the most. It was the love she still held for her husband, Nick, who died in 1994. “I never imagined I could live this long without him,” she had told me earlier in the day. It was the first time during the interview I detected a change in her voice. On the other end of the line, salty, pesky tears stung my eyes.
You see, Mrs D once worked in a bank. That’s where she met Nick. They became engaged and married during World War II and went on to live in Townsville where she worked with Qantas, as a clerk, until “peace was declared and the boys came home from the war.”
She still lunches a few times a year, at Brisbane’s Sofitel Hotel, with three of the surviving staff members from her bank days. “There were eight of us, but most of them are gone,” she said.
Her older sister and younger brother have also died. “It’s a terrible thing. You miss that link with your family,” she said.
But most of all, she misses Nick.
“Every night I have a glass of champagne, and I raise that glass to the empty seat beside me to keep his memory alive,” she said.
Love. It’s a many splendoured thing. Mrs D knows it. And I think we all do, too.


text:
welcome back to madridman.
I do the metro/bus at minimum as most of the time with a car or walk. However, having lived in the city and visiting now at least once a year, the public transport is very good. The site for trips in English is herehttps://www.metromadrid.es/en/viaja_en_metro/trayecto_recomendado/index.html
For the tickets if staying in Madrid proper the metrobus is ok; for the other trips you mentioned you need separate ticket as such. However, take a look at the Tourist Card; this allows trips in Cercanias suburbian trains either in zone A within Madrid proper or Zone T on the whole cercanias network, again for Cercedilla good to take the C2 from Chamartin, for Avila, Toledo, Segovia you need a separate ticket and train, and for me going to Segovia on the Sepulvedana bus line is better than the train as it leaves you closer to the city center.
For the train to Avila, Toledo, Segovia use the RENFE travel trip journeyhttp://www.renfe.com/EN/viajeros/
La Sepulvedana bushttps://www.lasepulvedana.es/indexcentro.php?lang=en
hope it helps

domain: travel


text:
Al-Doms has released his newest EP ‘New Day’. Being one of Virginia’s most respectable artists, the highly anticipated project has long been long sought after. Compiled of 6 songs Al-Doms describes the project with the following words; “The main message is to get up off ya ass, and don’t waste your potential. The chosen ones choose themselves so it’s all about choosing yourself.” ‘New Day’ also features the hit single ‘Pray’ which has arguably one of the best visuals from Virginia of the year. Check out Al-Doms newest project here.
Follow The Perfect Play on TwitterMy Tweets
As expected Frank Ocean's PrEP+ delivered a turnout that had many raving and excited for the next event. As stated in our previous post the event is designed for a no judgement zone for 𝐄𝐕𝐄𝐑𝐘𝐎𝐍𝐄 to come have fun and dance and although no phones allowed was listed on the house rules obviously with it being 2019 someone was able to get a phone in and capture footage of Frank Ocean debuting some new music Here is a brief snippet, head over to our site for more now.
Reese Laflare is back with a new song & visual ‘Hol Up’ Head over to our site now to check out the ‘Kill Bill’ inspired visual 🎥: @at.lasproductions
The “Vibe God” is back again with another polished album. On his debut album “Still Up All Night” we saw the Brooklyn native provide a small token of his creativity, however this time around Eli Sostre puts his smooth melodic sound and unique production choice on full display. Head to our site now for more info.

domain: entertainment


Wayfindr interacts with beacons to provide audio navigation in crowded areas
London Underground is providing a test site at Euston Station for a digital navigation system to help blind and partially sighted people find their way around urban environments.
Named Wayfindr, it uses a prototype smartphone app to interact with beacon technology and provide audio directions.
The technology has been developed by the Royal London Society for Blind People’s (RLSB) Youth Forum and digital product studio ustwo, and London Underground commissioned the trial to discover whether the system could work reliably across its network. This follows a pilot at the smaller, less busy Pimlico station early this year.
David Waboso, London Underground’s capital programmes director, said the project is aimed at helping vision-impaired people travel more independently.
Dr Tom Pey, chief executive of RLSB and chair of Wayfindr, said: “Smartphones have revolutionised the lives of blind people, giving us a level of independence that 20 years ago we couldn’t have imagined. What makes Wayfindr so strong is the focus on smartphones, meaning blind people don’t have to spend hundreds of pounds on different gadgets - they have everything they need in their pockets.
“I am excited for our young people to be at the forefront of making London the most accessible city in the world, through the Wayfindr Standard.”
Wayfindr is currently supported by a $1 million grant from Google as part of its Google Impact Challenge: Disabilities programme, which invites applications from projects that seek to solve problems for people living with disabilities, through technology.
It plans to run trials in other urban settings, and to launch a set of guidelines for audio navigation of visually impaired people early next year. Named the Wayfindr Standard, it will provide support for the developers of digital navigation services and owners of the relevant locations.
Image from Wayfindr through Flickr


I like playing War Blunder, but that's kind of a /vx/ topic. Here's a picture of the time that I tried to apply dazzle camouflage to my Churchill mk III. The idea was to pretty much just rape the viewer's eyes to the point where it's harder to discern where the weakpoints actually are.
Also, how do you feel about armored cars?
When I played Planetside 2 people liked to come up with fun sci-fi ideas for tanks. Sadly the developers were either lazy or understaffed and didn't get why WoT was beloved at the time.>>257194
Armored cars are sorely underrated, like IFVs (which are the backbone of any modern army but don't get a lot of hype). They're better suited than tanks for conducting patrols and supporting infantry. Beware that the Jews use them too: https://www.military.com/defensetech/2018/06/04/chunky-new-armored-vehicles-mantis-look-space-buggies.html
Historically though, armored cars had fun designs.https://www.youtube.com/watch?v=Ur3kRYAOFMc
THANK YOU MARVIN>>257143
How do you feel about heavily armored assualt trains?
THANK YOU MARVIN>all
How viable would a 150mm main cannon on a main battle tank be? What downsides would this have, what changes to accomodate the big gun would be required?
Russia and France are both considering it:https://nationalinterest.org/blog/buzz/could-russias-152-millimeter-cannon-be-added-armata-tank-25182https://www.thedrive.com/the-war-zone/26170/france-tests-huge-140mm-tank-gun-as-it-pushes-ahead-with-germany-on-a-new-tank-design
The bigger the shell, the more versatile it is; it can more easily destroy enemy armor as well as bunkers. 150mm might even be enough to mount a tactical nuclear warhead. The biggest downside is always ammo capacity. A gun is useful only so long as it has rounds to fire and bigger rounds mean you have a lot fewer. Consider that the .45 Colt 1911 was the standard-issue sidearm but had magazine capacities of only 7 rounds, and extended magazines are too big; nowadays the 9mm Sig-Sauer M17 is used as it has a capacity of 17(?) rounds which is a big difference. https://www.youtube.com/watch?v=jbqNzjzJcC4
I hear the US once tested nuclear tank projectiles and found that they work excellently. How true is this?
Also who cares about ammo storage when you can just get more ammo from ammo trucks?
Steel isn't strong. Flesh is stronger>>257280
I know that we made nuclear artillery and a batshit crazy nuclear bazooka.
It's certainly possible but not really practical. In terms of "micro" nuclear devices, the "Davy Crockett" recoilless rifle, CS-style place-and-set bombs and even larger-bore howitzer nuclear shells (the U.S. produced a lot of 8-inch shells) are more efficient in yield and cost due to how nuclear physics operates. As far as I know this was the smallest nuclear shell designed: https://en.wikipedia.org/wiki/W48
As for the Russians, the smallest shell they produced was the ZBV3 shell. So technically, an up-armed Armata could fire one but it's unlikely. Not only are SPGs better suited for the task than tanks but tactical ballistic missiles are generally a better option nowadays.https://www.rbth.com/defence/2017/04/20/why-russia-can-but-will-not-fire-nuclear-shells-from-an-armata-tank_747106
Your second question is obviously from ignorance as you've never even seen a tank operation in a game like Arma 3. Logistics vehicles are vulnerable targets and you don't want to be sitting in a field for ten minutes while crew carry shells from the truck to your tank. It's like asking "on a deep patrol why doesn't one guy carry all the food and water so dedicated soldiers are lighter?" If he gets taken out then everyone else is out of supplies. An army runs on its stomach.
I will admit I'm new to all of this.
By the way, why do tanks have Compensators on the end of their barrels instead of silencers for night operations? Don't they want to be stealthy? I am joking, I know nothing about tanks but I know enough to know silencers wont help a gun that big and loud.
Muzzle brakes mitigate felt recoil. That may be useful for a number of reasons. When the gun fires, it isn't static. The recoil move it backwards into the turret. With a muzzle brake, it may not need to move back as far which can allow you to utilize a smaller turret or give the crew more room to operate. It may also allow you to use a more powerful gun that you may normally have been able to use.>>257309
The Pershing's gun has a greater diameter than Panther F's , although the panther shoots higher velocity rounds that have superior penetration.
dumping some tanks
I got 2k hours in war thunder. It is pretty bad, but I like the game too much. We should run a /mlpol/ squad someday.
As much as people say it won't happen. I bet if the west goes to auto loaders we will start to see more potential for bigger tank rounds.
Big tank rounds are cool but I think that smaller-calibre "machine cannons" aren't given the attention they deserve. In terms of raw destructive potential they are incredible. MORE DAKKAhttps://www.youtube.com/watch?v=4UolMYY7QaA
57mm with auto loader rules.
And this is the original. (Art isn't mine)
I'll make a thread in /vx/ the next time that they do some big event. What nations do you play? I've gotten as high as the King Tigers in Germany, the churchill Mk VII in Britain (although I have the Black Prince and Strv 81), and the Hellcats in USA.
I play USA, Germany, and USSR. I got up to the T-62 in USSR, all US tanks except M1a2, and up to the Leopard 2A4 for Germany.
>>257332>Muzzle brakes mitigate felt recoil. That may be useful for a number of reasons. When the gun fires, it isn't static. The recoil move it backwards into the turret. With a muzzle brake, it may not need to move back as far which can allow you to utilize a smaller turret or give the crew more room to operate. It may also allow you to use a more powerful gun that you may normally have been able to use.
Modern tanks rarely have muzzle brakes as those complicate use of sabot rounds.
Swedish Meatball goes bljööp!


text:
Hawaiian Sweet Bread. I submitted this to Gooseberry Patch, Cozy Home cookbook, pg 10. I usually embellish with grape cluster and leaves made from the dough on top of round loaf.
Cucinando..( cooking )
Signature Design by Ashley Darcy - Salsa Contemporary Upholstered Chair and Ottoman with Tapered Wood Legs - Del Sol Furniture - Chair & Ottoman Phoenix, Glendale, Tempe, Scottsdale, Arizona
For the Home
http://cucinadriano.blogspot.com: Zuppa d'Aragosta _ women with the bottle of Calvad...
Luoghi da visitare
Maryland Style Hot and Spicy Crab Dip
Appetizers, Horderves & Snacks
dips spreads butters
Maryland Hot Crab Dip - a creamy delicious dip thick with lumps of sweet crab. Makes any day a party! At littlemisscelebration.com
Best crab dip recipe. Period. Old Bay's Creamy Crab dip recipe...don't edit the recipe one iota.

domain: food


text:
Ask 10 questions to the text
1.8 Look at the picture and say what is what? Where can they be used?
Chemical dishes: funnel, flask, plastic glass, gradated cylinder, test tube, automatic pipet, spouts.
Make your investigation. Design a plan for preparing caustic potash solution using all pictures from Ex.1.8
II Text for additional reading
In scientific research, data is gathered. In biology laboratories, mathematical data is' also used. To be understood easily and fully everywhere in the world, there must be one system used by scientists. That's why, in such research, the IS (International System of Measurement) standards are used. The metric system is commonly used, in which the meter is the unit of length, the gram is the unit of mass, and the second is the unit of time. Among these, some that are used especially in the biology laboratory are as follows:
The unit of length is the meter. Other units are arranged in relation to the meter. For instance, 1 m=100 cm; centi - means 1/100, so a centimeter is 1/100 meter. That means there are 100 centimeters in 1 meter.
Mass is the amount of matter in an object. The units of mass are the gram and kilogram. 1kg=1,000 g = 1,000,000 mg.
Volume is the amount of space an object occupies. Its unit is the liter.
The unit of time is the second. In a minute there are 60 seconds, and one hour is 60 minutes.
To measure temperature, there are three familiar scales: Celsius (°Q, Fahrenheit (°F) and Kelvin (K). The unit of temperature commonly used all over the world is the degree Celsius (°C).
Besides the microscope, dissection kits, certain chemicals and dyes, and models and posters, the glassware above is commonly used for practical work in the biology laboratory.
The laboratory has the potential to be either a safe place or a dangerous place. The difference depends on your knowledge of and adherence to safe laboratory practices. As a result, it is very important that you read the information provided here. A few basic rules for working safely in
the laboratory include the following:
1 - Always keep your work area clean.
2 - Wear proper safety clothing.
3 - Wear proper eye protection.
4 - Read all directions for an experiment several times.
5 - Follow the direction exactly as they are written.
6 - Be careful not to spill any material in the lab.
7- Always wash your hands before and after each experiment.
8 - Do not eat or drink in the lab.
Date: 2016-03-03; view: 1490

domain: chemistry


text:
- Research article
- Open Access
Hypothalamic FTO is associated with the regulation of energy intake not feeding reward
BMC Neuroscience volume 10, Article number: 129 (2009)
Polymorphism in the FTO gene is strongly associated with obesity, but little is known about the molecular bases of this relationship. We investigated whether hypothalamic FTO is involved in energy-dependent overconsumption of food. We determined FTO mRNA levels in rodent models of short- and long-term intake of palatable fat or sugar, deprivation, diet-induced increase in body weight, baseline preference for fat versus sugar as well as in same-weight animals differing in the inherent propensity to eat calories especially upon availability of diverse diets, using quantitative PCR. FTO gene expression was also studied in organotypic hypothalamic cultures treated with anorexigenic amino acid, leucine. In situ hybridization (ISH) was utilized to study FTO signal in reward- and hunger-related sites, colocalization with anorexigenic oxytocin, and c-Fos immunoreactivity in FTO cells at initiation and termination of a meal.
Deprivation upregulated FTO mRNA, while leucine downregulated it. Consumption of palatable diets or macronutrient preference did not affect FTO expression. However, the propensity to ingest more energy without an effect on body weight was associated with lower FTO mRNA levels. We found that 4-fold higher number of FTO cells displayed c-Fos at meal termination as compared to initiation in the paraventricular and arcuate nuclei of re-fed mice. Moreover, ISH showed that FTO is present mainly in hunger-related sites and it shows a high degree of colocalization with anorexigenic oxytocin.
We conclude that FTO mRNA is present mainly in sites related to hunger/satiation control; changes in hypothalamic FTO expression are associated with cues related to energy intake rather than feeding reward. In line with that, neurons involved in feeding termination express FTO. Interestingly, baseline FTO expression appears linked not only with energy intake but also energy metabolism.
Human studies show a strong association of a single nucleotide polymorphism in the FTO gene with obesity and diabetes in diverse ethnic groups [1–7]. Common intronic variants in this gene have been linked with increased body weight and adiposity ; e.g., individuals homozygous for the rs9939609 allele, weigh ~3 kg more than those with low-risk alleles . High-risk variants carry a population-attributable likelihood of overweight and obesity exceeding 20% [3, 6]. Aside from the association with BMI, variations in the FTO gene have been linked with glucose metabolism [1, 9], type 2 diabetes [10, 11], distribution of subcutaneous fat and amount of liver fat, as well as non-visceral and visceral adipose tissue . FTO codes for 2-oxoglutarate-dependent nucleic acid demethylase that likely plays a role in oxidative demethylation of 3-methylthymine and 3-methyluracil in single-stranded DNA and RNA .
Little is known about the nature of the association between FTO and BMI; only a few basic research studies have been performed on laboratory animals. Fontanesi et al. found that FTO is associated with intermuscular fat deposition in pigs . FTO mRNA is present in many tissue types, but its most abundant expression was detected in the brain, particularly, in hypothalamic nuclei which govern feeding [15, 16]. In rodents, starvation modifies FTO mRNA levels detected with real-time PCR (rtPCR) in the entire hypothalamus and in the arcuate nucleus (ARC), however both up- and downregulation have been shown, thus, FTO expression alterations may be dependent on the severity of starvation or other factors [16, 17, 15]. These changes are independent from leptin . Generation of the knock-out (KO) model was a crucial step in understanding FTO's role in body weight control. KO mice displayed decreased body weight despite consuming more standard chow than wild type (WT) controls; this effect persisted from post-natal day 2 onwards. In addition, FTO mRNA was found to colocalize with ARC proopiomelanocortin (POMC) , which gives rise to, among others, a satiety factor alpha-melanocyte stimulating hormone and reward mediating beta-endorphin.
Animal experiments performed to date have not addressed the key issue of whether FTO's involvement in weight gain is based solely on energy-related mechanisms or there is also a reward component. The current project was designed to characterize the relationship between FTO and obesity through feeding models, molecular analyses and functional neuroanatomy in mice. Special attention was given to the hypothalamus as - aside from primarily regulating energy intake - it mediates feeding reward [19, 20].
We investigated whether FTO mRNA levels respond to a brief fast. Since overeating, especially of palatable tastants, serves as an obesogenic factor, we studied the effect of short-term (48 h) overconsumption of sucrose or fat on hypothalamic FTO expression. We examined whether body weight increase induced by long-term exposure to sugar has any effect. Furthermore, individual preferences for fat versus sucrose were correlated to the baseline FTO expression. Since same-weight mice exhibited inherent differences in their propensity to consume energy especially when diverse diets were offered, we compared the baseline expression profiles in individuals that ate more to the ones that ingested fewer calories and we employed a similar rat model to confirm our findings. Organotypic cultures of the hypothalamus were used to study FTO mRNA levels following the addition of a satiating amino acid, leucine .
We performed in situ hybridization (ISH) to compare FTO expression in strictly reward-related sites to that in the hypothalamus, a region involved in both feeding for energy and reward [19, 20]. As FTO was found to coexpress with POMC, double ISH-immunohistochemistry (IHC) was performed to determine whether FTO colocalizes with another hypothalamic feeding regulator, the anorexigenic peptide, oxytocin [22, 23]. Finally, using IHC detection of an immediate-early gene product, c-Fos, we studied activity of FTO neurons at initiation vs. termination of feeding.
Male C57BL/6J mice (Scanbur, Sweden) were housed individually or in groups of two (PCR Exp. 1, 2 and 3) in macrolon cages with LD 12:12 (lights on at 0700). The animals were 12 weeks old and they weighed ca. 28 g at the beginning of the experiment. Water and chow (Lactamin, Sweden) were available ad libitum unless noted otherwise. Procedures described herein received a prior approval from the Uppsala Animal Welfare Committee.
Experiment 1. Hypothalamic FTO expression following 16-h deprivation
Food was removed just before the onset of darkness and mice (n = 8) were decapitated between 1000 and 1100 on the next day. The controls (n = 8) had food ad libitum.
Experiment 2. Hypothalamic FTO expression following 48-h consumption of palatable sucrose or Intralipid
Mice were given a bottle of 10% sucrose or 4.1% Intralipid in addition to chow for 48 h; controls had chow only (n = 8/group). Intralipid (Fresenius, Sweden), a palatable lipid emulsion of soybean oil, glycerol and egg yolk phospholipids, has been used in experiments utilizing liquid diets [24, 25]. Sucrose and Intralipid were isocaloric (0.4 kcal/g), whereas the energy content of chow was 3.6 kcal/g. The solutions were similar in palatability: each mouse ingested on average 8.1 kcal of Intralipid and 7.4 kcal of sucrose daily. Total caloric intake per mouse was 10.3 kcal in the chow group, 14.1 kcal in the sucrose group and 12.9 kcal in the Intralipid group. Mice were decapitated after 48 h (between 1100 and 1200).
Experiment 3. Hypothalamic FTO expression upon increased body weight
Mice were given 10% sucrose in addition to chow for 3 weeks; controls had chow only (n = 8/group). The initial body weights did not differ (controls: 27.6 ± 0.3 g; sucrose: 27.8 ± 0.5 g). After 3 weeks of chow and sucrose intake, mice weighed 32.1 ± 0.4 g, whereas the chow controls weighed 29.5 ± 0.6 g (P < 0.05; t-test). Energy intake was similar to that described for the corresponding groups in Exp. 1. Mice were decapitated after 3 weeks (1100-1200).
Experiment 4. Hypothalamic FTO expression in mice differing in preference for sugar vs. fat
Mice were given 10% sucrose and 4.1% Intralipid in addition to chow for 7 days. During the first two days, animals got accustomed to tastants. Subsequently, according to the standards applied in previous reports [26, 27], the mice were divided based on the 5-day preference profile: 1) sucrose-preferring (n = 7), 57.0 ± 2.2% of sucrose + Intralipid calories came from sucrose; (2) fat-preferring (n = 7), 39.2 ± 3.0% calories came from sucrose; and (3) neutral (n = 7), sucrose was the source of 46.9 ± 1.9% calories. Sucrose and Intralipid were later removed and only chow was given for 21 days ("washout") so that gene expression levels at endpoint were not affected by consumption of palatable tastants. The animals were decapitated (1100-1200). Body weights at the end of the palatable tastant availability and at the end of the "washout" phase did not differ between the preference groups.
Experiment 5. Hypothalamic FTO expression in mice differing in calorie intake upon availability of a diverse diet
The set-up in Exp. 4 provided a diverse diet that included standard chow, palatable sucrose and Intralipid. Although animals displayed different propensities to ingest sucrose and Intralipid, some of them - regardless of preference - ingested overall (sucrose+Intralipid+chow) more energy than others. The "small eaters" (n = 10) ate daily 11.3 ± 0.4 kcal of all tastants while the "big eaters" (n = 11), 14.4 ± 1.2 kcal (P < 0.05; t-test). The difference in the amount of consumed food was significant in the environment rich in diverse foods. The initial body weight did not differ between the groups ("small eaters": 27.2 ± 0.4 g; "big eaters": 27.5 ± 0.3 g). The 7-day exposure to palatable diets did not induce changes in body weight (29.0 ± 0.4 g and 28.3 ± 0.3 g, respectively; P = 0.287). Once palatable liquids were removed and the animals had only access to chow for 21 days, energy intake did not differ between groups during this "washout phase" although a slight trend remained (cumulative intake for big eaters: 241.8 ± 14.1 kcal vs small eaters: 221 ± 12.6 kcal; P = 0.160) and neither did body weights (P = 0.605) at decapitation.
Experiment 6. Arcuate hypothalamic FTO expression in rats that differ in calorie intake upon availability of a diverse diet
This experiment was included to examine whether the FTO expression pattern in the "big/small eaters" paradigm can be detected in rats. Male Wistar rats, housed individually with free access to chow and water, received for 10 days a 30% sucrose solution (n = 10), a bowl of lard (n = 10), or both tastants (n = 15). Animals increased their caloric intake when presented with the palatable diets (229 ± 3.4 kcal/kg on chow and 276 ± 5.7 kcal/kg on palatable diets; P < 0.0001). The animals were divided into "big eaters" (n = 17) and "small eaters" (n = 17): 249 ± 3.0 kcal/kg for the "small eaters" and 302 ± 5.9 kcal/kg for the "big eaters". Body weights did not differ between the groups following the exposure to palatable diets or at endpoint (P = 0.411 and 0.640, respectively). Arcuate hypothalamic FTO expression was studied with rtPCR.
Experiment 7: FTO expression in organotypic cultures of the hypothalamus treated with anorexigenic leucine
Organotypic hypothalamic slice cultures were prepared as previously described . Briefly, 21-day-old male C57BL6 mice were decapitated, the brains were removed under sterile conditions and transferred to the Gey's Balanced Salt Solution (GBSS) (Biological Industries, Israel) containing 25 mM glucose. Hypothalami were dissected and sliced into 300-μm thick sections using the McIlwian tissue chopper. Slices from each brain were placed separately on semiporous membranes (Millipore) inserted into six-well culture plates (Sarstedt) containing 1.2 ml of the serum-Opti-MEM medium in each well. Culture medium was a mixture of Opti-MEM (50%; Gibco), horse serum (25%) and Hanks' Balanced Salt Solution (HBSS) (25%; Gibco) supplemented with 50% D(+) glucose monohydrate (25 mM, Merc). Growing medium of the experimental group (n = 7) was supplemented with 0.3 μg leucine; controls (n = 7) had no leucine added. The amount of supplemented leucine was based on the previous report utilizing central administration of this amino acid to reduce food intake . Cultures were maintained at 37°C in a humidified atmosphere of 5% CO2. Medium was changed daily. After 48 h FTO expression was studied with rtPCR.
Brains were excised, hypothalami dissected, immersed in RNAlater (Ambion, USA), kept at room temperature for 2 h and, thereafter, stored at -80°C until further processed. Samples were homogenized and RNA prepared as previously described [15, 29] [see Additional file 1].
Relative expression levels of 6 housekeeping genes (HKGs) and genes of interest were determined with quantitative rtPCR. Each reaction, with a total volume of 20 μl, contained 20 mM Tris/HCl, 50 mM KCl, 4 mM MgCl2, 0.2 mM dNTP, DMSO (1:20) and SYBR Green (1:50000). Template concentration was 5 ng/μl and the concentration of each primer was 2 pmol/μl. Primers were designed with Beacon Designer (Premier Biosoft) using the SYBR Green settings. Sequences are presented in Additional file 1. All rtPCR experiments were performed in duplicates; a negative control for each primer pair and a positive control with 5 ng/μl of genomic DNA were included on each plate. Amplifications were performed with 0.02 μ/ml Taq DNA polymerase (Biotools, Sweden) under the following conditions: initial denaturation at 95°C for 3 min, 50 cycles of denaturing at 95°C for 15 sec, annealing at 52.8-60.1°C for 15 sec and extension at 72°C for 30 sec.
Data analysis and relative expression calculations
Analysis of rtPCR data was performed as previously reported . MyIQ 1.0 software (Bio-Rad) was used. Primer efficiencies were calculated using LinRegPCR  and samples were corrected for differences in primer efficiencies. The GeNorm protocol described by Vandesompele et al.  was used to calculate normalization factors from the expression levels of the HKGs. Grubbs' test was used to remove outliers (6 outliers were removed) [32, 33]. Differences in gene expression between groups were analyzed with ANOVA followed by Fisher's PLSD test where appropriate. P < 0.05 was used as the criterion of statistical significance.
ISH detection of FTO in sites involved in hunger versus reward
Four mice were anaesthetized with pentobarbital and perfused with 5 ml 0.9% NaCl followed by 50 ml 4% paraformaldehyde in 0.1 M phosphate buffer. Perfusions took place between 1100 and 1200. The brains were immersed in the same fixative overnight at 4°C. Coronal sections were cut at 50 μm with a Vibratome and processed as free-floating sections.
The FTO cDNA clone was obtained from Invitrogen. Plasmid DNA preparation was done with the JETstar 2.0 Plasmid Purification Midi Kit/50 (Genomed, Germany). The clones were sequenced (MWG, Germany) and confirmed to contain 773 bp of the 3' UTR of the mouse FTO transcript. 20 μg of plasmid DNA was lineraized by digestion with 30 U SpeI (Fermentas, Latvia) for 3 h. Ribo probes were synthesized using 1 μg template, RiboLock Ribonuclease Inhibitor (Fermentas, Sweden) and 40 U RNA polymerases in the presence of digoxigenin-11-UTP (Roche). Probe quantification was performed with a NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies). The synthesis of the FTO RNA probe was performed according to the previously described protocol . FTO mRNA was detected in the tissue as described before  and the full protocol can be found in Additional file 1. Evaluation of FTO expression was based on the visual inspection; the following scale was applied: 0, not detectable; +, weak; ++, moderate; +++, high. Every other section was used for the ISH.
Detection of oxytocin in FTO neurons
Following the completion of the ISH procedure, every other PVN section was used in the IHC staining to detect oxytocin. Sections were rinsed in TBS, incubated in 3% H2O2/10% methanol for 10 min. The tissue was incubated 24 h in the rabbit-anti-oxytocin antibody (1:15000; Chemicon), followed by incubations in the goat-anti-rabbit (1:400; Vector Laboratories) and avidin-biotin complex (1:800; Vector Laboratories) for 1 h. Peroxidase in the tissue was visualized with 0.05% diaminobenzidine and 0.01% H2O2 was used to catalyze the reaction. Rinsing steps were done with TBS. The vehicle for antibody incubations was 0.25% gelatin and 0.5% Triton X-100 in TBS. Sections were analyzed visually and the percentage of oxytocin neurons colocalizing with FTO was determined.
Detection of c-Fos in FTO neurons at feeding initiation and termination
Mice (n = 10) were food-deprived for 16 h. Chow was returned at 1000. Deprivation-induced meal was completed within ~30 min (each mouse ingested 2.0-2.4 g chow). Five mice were perfused at the time corresponding to the initiation of feeding, whereas the other five at the time corresponding to the termination of consumption. Since the maximum c-Fos IR occurs 1 h after the onset of neuronal activity , animals were perfused 1 h after food presentation (initiation)or 1 h after meal completion (termination).
The ISH protocol was as described above. In IHC, the modifications included the use of the goat-anti-Fos (1:1500; Santa Cruz) and rabbit-anti-goat (1:400; Vector Laboratories) antibody. The percentage of Fos-positive FTO in the PVN and ARC was determined and values were compared with Student's t-test (significant when P < 0.05).
Exposure to palatable, isocaloric sucrose or Intralipid for 48 h did not affect FTO expression (Fig. 1a); the animals had the same body weight. When mice were given 3-week access to sugar, which caused a greater body weight increase than intake of chow alone (32.1 ± 0.4 g and 29.5 ± 0.6 g, respectively; P < 0.05), FTO mRNA levels were still the same in both groups (Fig. 1b). Also animals that had distinct preference profiles for sugar vs. fat did not differ in the baseline FTO expression (Fig. 1d). In the three aforementioned models, changes in expression of other reference genes known for their involvement in food intake control could be detected. Those included kappa (KOR) and mu (MOR) opioid receptors, dynorphin (DYN), proopiomelanocortin (POMC), melanocortin receptor-4 (MC4R) and melanin concentrating hormone (MCH).
In contrast, FTO expression was increased in deprived animals (Fig. 1f). Treating organotypic cultures of the hypothalamus with anorexigenic leucine caused downregulation of FTO mRNA as well as of two orexigenic genes, NPY and ORX (Fig. 1c). Finally, the propensity to eat less during the exposure to a self-composed, diverse and palatable diet was associated with higher FTO mRNA levels. During the exposure, "small eaters" ate fewer calories than "big eaters" did (11.3 ± 0.4 kcal and 14.4 ± 1.2 kcal per day, respectively; P < 0.05) and, after the 3-week "washout" phase of chow intake (trend of elevated calorie intake in big eaters; P = 0.160), they had ~50% higher FTO mRNA content (P = 0.004; Fig. 1e). There was no change in expression of several reference genes, including DYN, POMC, neuropeptide Y (NPY), Agouti-related protein (AGRP) and MC4R (Fig. 1e). Applying a similar paradigm in rats resulted in the same outcome for the FTO mRNA in the hypothalamic ARC, i.e. "small eaters" had an elevated level of FTO (P = 0.030; Fig. 1e).
ISH detection of FTO mRNA revealed expression primarily in the hypothalamus. Some sites linked with reward, such as the ventral tegmental area (VTA) and nucleus accumbens (Acb), had very little or lacked the FTO signal, whereas others, e.g., the bed nucleus of the stria terminalis and parabrachial nucleus, expressed moderate levels of FTO (Table 1; Fig. 2). Double ISH-IHC analysis showed that FTO mRNA is present in many neurons synthesizing the anorexigenic peptide, oxytocin: 55 ± 6.1% oxytocin neurons in the magnocellular subdivision of the PVN and 32 ± 5.8% in the parvocellular portion colocalized with FTO (Fig. 3). Interestingly, we found that approximately four times more FTO expressing cells in the PVN and ARC display c-Fos (they are "active") at the time corresponding to termination than to initiation of consummatory activity (Fig. 4). There was no difference between the two groups in the number of FTO cells (data not shown).
Consuming excessive amounts of foods, which serves as the main factor in the development of obesity (and may result in diabetes), is driven by either a perceived need to acquire calories (eating for energy in response to the hungry state) or hedonic aspects of eating (eating for pleasure) or the combination of both. Our data strongly suggest that FTO, the first gene contributing to common forms of human obesity [8, 35], plays a significant role in mechanisms responsible for the regulation of energy-related eating behavior.
This presumed involvement in hunger control is reflected by the neuroanatomical distribution of FTO visualized through ISH. This gene is scarcely expressed within the reward network: particularly low levels are found in the Acb and VTA which host, e.g., opioid and dopaminergic circuitry [36–39] mediating hedonics of food intake. On the other hand, very high expression was detected in hypothalamic regions, including the PVN, ARC and dorsomedial nucleus (DMH) that encompass key genes influencing hunger and satiety, such as NPY, AGRP, POMC and MC4R.
Distribution primarily outside the "reward network" does not exclude a possibility of this gene's involvement in reward processes, especially since the molecular components of the reward system (e.g. genes encoding opioid peptides and receptors) are dispersed throughout the CNS and present also in the hypothalamus . In fact, intake of palatable diets, especially those containing fat or sugar, modifies expression of reward-related genes in the hypothalamus; and reward mediators, e.g., opioid ligands, injected in this structure are particularly effective in changing intake of such foods [40–42]. However, in our experiments we have not found evidence linking hypothalamic FTO with feeding reward. Ingestion of palatable sugar or fat did not affect FTO expression, which suggests that FTO mRNA levels do not change upon exposure to palatable tastants in general and there is no special relationship between FTO and a particular palatable macronutrient (fat or sugar). This was seen following short- (48 h) and long-term (3 weeks) intake of rewarding tastants. The outcome is in line with our previous findings showing the lack of correlation in expression of FTO and reward-related genes, including DYN and enkephalin, in rat deprivation models . In addition, differences in animals' liking of sugar versus fat did not reflect any disparities in FTO gene activity profile. It suggests that the inherent preference for a specific macronutrient is not dependent on the baseline FTO expression in the hypothalamus. These data are of particular importance as they suggest that hypothalamic FTO's role is unrelated to eating for pleasure or inclination to choose one palatable macronutrient over another. As the mice maintained on sucrose for 3 weeks gained more weight than chow-fed controls, it also indicates that the development of overeating-driven obesity is not a causative factor in changes in FTO expression. This lack of change in FTO expression in the aforementioned models shows a striking difference from the response of other feeding-related "reference" genes studied herein, including opioids, melanocortins and MCH. Obviously, one cannot rule out the possibility that FTO outside the hypothalamus may affect or be affected by the feeding variables considered herein, including reward and preference, but hypothalamic FTO shows a strong association only with energy intake control.
Three independent studies have shown that depriving rodents of food influences FTO mRNA content in the brain [15–17]. In mice, fasting was reported to decrease expression of this gene, however, starvation applied in those studies was severe as it lasted 40-48 h [16, 17]. Upregulation has been detected in rats. In the current study, a short-term, overnight fast, upregulated FTO mRNA. The data confirm that FTO is sensitive to a current energy status of the organism, probably in terms of both energy acquired and stored; but the results also point to the necessity of defining parameters accompanying deprivation of various magnitudes and timeframes that shape the FTO gene activity.
The Fos experiment showed that >4 times more FTO neurons in the PVN and ARC were "activated" at feeding termination than initiation. Activity of FTO expressing cells at the end of a meal suggests FTO's involvement in feeding inhibitory mechanisms. In fact, c-Fos IR is increased in the PVN and ARC of rodents following 1-h intake of caloric but not non-caloric tastants . Furthermore, an upward trend in neuronal activity in hypothalamic cells synthesizing anorexigenic peptides has been reported: a greater percentage of Fos-positive neurons synthesizing oxytocin and alpha-melanocyte stimulating hormone can be detected at feeding termination [44, 45]. Noteworthy, FTO is expressed in PVN neurons that produce oxytocin, which is released within the CNS in response to such satiation cues as increased stomach distention and high plasma osmolality. This colocalization occurs in magno- and parvocellular cells, which implies that FTO may influence the activity of oxytocin neurons that project to the posterior pituitary (magnocellular) as well as to sites linked with feeding control, including the nucleus of the solitary tract and dorsal motor nucleus of the vagus (parvocellular) . Our oxytocin colocalization results along with the previous report regarding POMC  indicate that FTO may be involved in mechanisms that end consummatory activity, thus, those that are closely tied with meal size (therefore, amount of ingested energy) control. A previously reported correlation in the expression of FTO and energy-related gene encoding galanin-like peptide supports this concept .
The question remained as to whether the role of FTO in satiety-related hypothalamic neurons, such as those positive for POMC or oxytocin, is to increase or decrease their activity. Addition of leucine, which in vivo reduces feeding and activates anorexigenic neurons synthesizing POMC , to hypothalamic organotypic cultures produced downregulation of FTO expression. This suggests that nutrient cues mediating satiation decrease FTO mRNA levels while increasing activity of the satiety network (e.g., POMC cells), hence, there is the inverse relationship between FTO and satiety.
The first studies with the use of the FTO KO murine model showed that the role of FTO in energy intake and metabolism regulation goes beyond short-term control of feeding. Surprisingly, FTO KO mice weigh less even though they exhibit relative hyperphagia . Our "big/small eaters" experiment further supports the findings obtained in the KO mice. This experiment was inspired by the observations that diversity of foods propels energy intake beyond levels required to satisfy energy needs. Interestingly, under such conditions, some individuals display a significantly higher degree of hyperphagia than others [19, 45]. The "big eaters" - characterized by lower FTO expression (P = 0.004) - weighed the same as the "small eaters", even though during the several-day diverse food exposure period they ingested ca. 22% more energy and a slight trend of higher energy intake (P = 0.160) remained throughout the subsequent exposure to regular chow. Our data indicate that the higher energy intake that does not translate to increased body weight may be accompanied by downregulation of the FTO gene in the hypothalamus. This is in concert with the conclusion reached in the FTO KO mouse studies that not only feeding, but also energy metabolism and expenditure are affected by FTO.
Changes in hypothalamic FTO mRNA levels are associated with a deprived state as well as with the presence of the amino acid leucine that promotes satiety and feeding termination. In addition, c-Fos IR of FTO neurons in the ARC and PVN increases at the end of consummatory activity; and FTO co-localizes with anorexigenic oxytocin in the PVN. It allows us to speculate that hypothalamic FTO may be involved in the regulation of energy intake, possibly by affecting feeding termination mechanisms. Distribution of FTO mRNA mainly in hunger/satiation-related sites and lack of changes in FTO expression upon exposure to palatable tastants call into question a possible involvement of FTO in the reward component of feeding. Finally, the inherent propensity to eat more without a change in body weight is associated with low hypothalamic FTO mRNA levels. In contrast, long-term palatability-driven overconsumption (irrespective of the aforementioned individual propensity to eat different amounts of food) that leads to increase in body weight does not affect FTO expression in the hypothalamus.
Overall, our molecular and histological data strongly link hypothalamic FTO with the regulation of feeding for calories not for reward; the molecular analyses also point to a possible role of this gene in energy metabolism.
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The studies were supported by the Swedish Research Council (VR, medicine), AFA insurance, Swedish Brain Research Foundation, Svenska Läkaresällskapet, Åhlens Foundation, Novo Nordisk Foundation, Göran Gustafssons Foundation, National Institute of Drug Abuse (R01DA021280), Systembolagets råd för alkoholforskning (SRA), and National Institute of Diabetes and Ingestive and Kidney Diseases (P30DK50456).
PKO - carried out the ISH experiments and tissue dissections, drafted the manuscript, RF - participated in the design of the molecular studies and design of the animal models, AMO - carried out double ISH-IHC staining, OS - carried out mouse PCR studies, JA - designed and carried out the rat FTO PCR studies, KJR - designed and carried out the organotypic tissue culture studies, ASL - participated in the statistical analyses and drafting the manuscript, HBS - participated in the design and coordination of the study and in drafting the manuscript. All authors read and approved the final manuscript.
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Additional file 1: Detailed PCR and ISH methodology. This file contains additional information pertaining to the PCR and ISH methodology applied herein. (DOC 64 KB)
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Olszewski, P.K., Fredriksson, R., Olszewska, A.M. et al. Hypothalamic FTO is associated with the regulation of energy intake not feeding reward. BMC Neurosci 10, 129 (2009). https://doi.org/10.1186/1471-2202-10-129
- Organotypic Culture
- Melanin Concentrate Hormone
- Diverse Diet

domain: medical


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According to Phys.Org, the robotos also function as “wireless two-way communications devices.” They allow inmates and guards to communicate with each other immediately and directly.
Arguably, this would also speed up response times during emergencies.
The one-month trial will cost better than $750,000. If it proves viable, the Asian Forum of Corrrections hopes to employ them permanently. It also hopes to fine tune them so they can carry out random weapon and contraband searches.
It does not require an excess of imagination to picture how wrong that might go.


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We asked six travellers about their experiences hunkering down Taiwan’s quarant hotels. Here’s what they had to say.Read More
Where to stay overnight and find your hostels or budget hotels in Taipei.Read More
5 amazing hotel choices for relaxing and recharging.Read More
Valentine’s Day is coming and here are Taiwan Scene’s hotel selections for you to spend the romantic day with your loved ones in Taipei.Read More
As the year’s end approaches, the streets of Taipei are filled with a festive atmosphere for the new year. Here are three trendy hotels that feature its own unique charm when it comes to celebration of new year!Read More
For the environmentally-conscious traveler, slow travel has become the new way to see the world. With a belief that travel should be sustainable and leave a positive impact on the environment and surrounding communities, globe trekkers are now choosing to visit places that practice environmental conservation, sustainable development, and the preservation of local culture and traditions.
For the founders of the Adagio hotel in Jinguashi, Taiwan, (“Adagio” meaning “in a slow tempo”) slow travel is also an art meant to be experienced leisurely with all five senses. Throughout the month of March, Adagio holds a series of events that allow guests to enjoy a sensory experience for the ears (musical performances), the eyes (art classes), the nose (fragrance and incense workshops), the mouth (wine tasting, for example), and the hands (plant designing classes).
The hotel is located high up in the mountainous roads, not far from the Golden Waterfall. A cozy vicinity with no cable, Adagio is a hotel that creates a sense of community, with a limited number of rooms and group activities that allow you to meet other guests.


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3 edition of The best science fiction of the year 12 found in the catalog.
The best science fiction of the year 12
|Statement||edited by Terry Carr.|
|The Physical Object|
|Number of Pages||350|
The life and strange surprising adventures of Robinson Crusoe
Computerised analyses of estimating inaccuracy and tender variability
Chiropractic approach to head pain
military in contemporary Soviet politics
Bt-Framework Users Hdbk
Guidance for walking judges
Bird studies for primary science in the National Curriculum
Manual of jurisprudence and co-operation of the Patrons of Husbandry
study of some physiological effects of vitamin G deficiency ...
Clinton and the Town of Kirkland
Workshop on Diquarks, Villa Gualino, Torino October 24-26, 1988
Made in Korea.
Apr 18, · The Best Science Fiction and Fantasy of the Year: Volume Twelve (12) (Best SF & Fantasy of the Year) [Jonathan Strahan] on inkpapery.icu *FREE* shipping on qualifying offers. The latest in a series that has been called “a must-read for fans of science fiction, fantasy, and short stories in general” Science fiction is a portal that opens doors onto futures too rich and strange to imagine.4/4(16).
Apr 18, · The Best Science Fiction and Fantasy of the Year, Volume Twelve - Kindle edition by Jonathan Strahan, Charlie Jane Anders, Scott Lynch, Yoon Ha Lee. Download it once and read it on your Kindle device, PC, phones or tablets.4/5(15).
Announcing the best books of the year. The Goodreads Choice Awards are the only major book awards decided by readers. Best Science Fiction. New to Goodreads. Get great book recommendations. Start Now.
Want to Read. and the essential nature of memory. The book’s decidedly weird science is actually grounded in recent laboratory research. Dec 13, · Ted Chiang is responsible for some of the best written science fiction in recent years, and his latest collection — the first since ’s The Story of Your Life and Others — brings together.
I have to say that this Volume One of The Best Science Fiction of the Year, edited by Neil Clarke just might be the best science fiction anthology that I have ever read. It is loaded with stories of deep space travel, other worlds, and alien intelligence - which is, of course, my favorite type of science fiction/5.
Aug 14, · The world’s best-selling science fiction novel of all time, and often described as the Lord of the Rings of science fiction. If you’ve never read a science fiction book before, don’t start here, but make it your fifth.
The Martian Chronicles. by Ray Bradbury. May 22, · Here are some of the best science fiction books inkpapery.icu's writers and editors have read and loved — an incomplete (but growing) list. Nov 29, · The editors of The Times Book Review choose the best fiction and nonfiction titles this year.
Book Review choose the best fiction and nonfiction titles this year. history and science of. May 10, · The Best Science Fiction and Fantasy of the Year: Volume Eleven (11) (Best SF & Fantasy of the Year) [Jonathan Strahan] on inkpapery.icu *FREE* shipping on qualifying offers.
The 11th volume in this much lauded series with incisive and genre-defining stories chosen my multi-award winning editor Jonathan Strahan. The internationally-acclaimed ‘Best Science Fiction and Fantasy of the Year /5(33). Nov 20, · These are the best science fiction and fantasy books of the year.
[A note from the future: Once you’ve finished working through this list, check out our picks for the best books of so far and the year-end list of the best sci-fi and fantasy of ]Author: Joel Cunningham. May 22, · Best Science Fiction of the Year: Volume One, The [Neil Clarke (Editor), Jeremy Arthur Amy Tallmadge] on inkpapery.icu *FREE* shipping on qualifying offers.
To keep up to date with the most buzzworthy and cutting-edge science fiction requires sifting through countless magazines/5(48). After winning Best Science Fiction in the Goodreads Choice Awards for his thrilling debut, The Martian, he receives the same honor for his second book.
Part space adventure, part exhilarating heist tale, Artemis follows young smuggler Jazz Bashara as she lands in the middle of conspiracy on the first and only city on the moon. Science fiction is a portal that opens doors onto futures too rich and strange to imagine.
Fantasy takes us through doorways of magic and wonder. For more than a decade award-winning editor Jonathan Strahan has sifted through tens of thousands of stories to select the best, the most interesting, the most engaging science fiction and fantasy to Released on: April 17, The Best Science Fiction of the Year #12 () The Best Science Fiction of the Year #13 () Terry Carr's Best Science Fiction of the Year () = Best SF of the Year #14 ().
Strong and varied offerings make this a must read for any type of science fiction fan. Head on over to Signal Horizon to see my full review and I am a huge fan of the "Years Best" collections of short fiction and recently editor Neil Clarke has entered the yearly "best of" collection ring with his series The Best Science Fiction of the Year/5.
Dec 21, · Here are 15 of our favorite science fiction and fantasy books that that hit bookshelves this year, stories about interstellar colonization, magical civilizations, and time-traveling murder mysteries.
Welcome to our round-up of the best books of the year. Discover the most talked about and praised books this year according to Barnes & Noble and media like The New York Times, Publishers Weekly, and The Washington inkpapery.icu explore the best books of the past decade to see what was popular throughout the years.
The Year's Best Science Fiction was a series of science fiction anthologies edited by Gardner Dozois until his death in The series, which is unrelated to the similarly titled Year's Best SF series, is published by St.
Martin's Press (Griffin). In the UK the series was entitled The Mammoth Book Of Best New Science Fiction and is published by Robinson. Jun 30, · Best Science Fiction of the Year and millions of other books are available for Amazon Kindle.
Enter your mobile number or email address below and we'll send you a link to download the free Kindle App. Then you can start reading Kindle books on your smartphone, tablet, or computer - no Kindle device required/5(19).
Oct 31, · Buy The Best Science Fiction and Fantasy of the Year: Volume Twelve: 12 (Best SF & Fantasy of the Year) by Jonathan Strahan (ISBN: ) from Amazon's Book Store. Everyday low prices and free delivery on eligible orders.1/5(1). The Nebula Awards are handed out by the Science Fiction & Fantasy Writers of America and their annual event celebrates the books of the previous year.
The Hugos are chosen by members of Worldcon and the event is named after Hugo Gernsback, the founder of the pioneering science fiction magazine Amazing Stories.
Note: Citations are based on reference standards. However, formatting rules can vary widely between applications and fields of interest or study. The specific requirements or preferences of your reviewing publisher, classroom teacher, institution or organization should be applied.
Oct 14, · The Washington Post will be publishing an ever-rotating list of some of the best science fiction we’ve read, populated by staff and reader suggestions and moderated by us here at Book World.
Nov 30, · Best books of the year The best science fiction and fantasy of Adam Roberts finds floods in Manhattan, magic in Paris and a shortage of electricity across the world.
Jun 07, · With The Best Science Fiction of the Year Volume One, award-winning editor Neil Clarke provides a year-in-review and thirty-one of the best stories published by 3/5.
The Best Science Fiction of the Year #13 is an anthology of science fiction short stories edited by Terry Carr, the thirteenth volume in a series of sixteen. It was first published in paperback by Baen Books in Julyand in hardcover and trade paperback by Gollancz in December of the same inkpapery.icu: Science fiction.
Dec 29, · It was a great year for books. Here are our favorites. Skip to main content The Verge homepage The best science fiction, fantasy, and horror novels of New, 11 comments. Dec 03, · Best books of the year Best science fiction and fantasy books of A year in which Kim Stanley Robinson took us beyond the stars in Aurora and.
Nov 16, · The end of marks the completion of this blog’s first year of existence, and what a year to start with: we can’t remember the last month period that gave us such a strong run of new science fiction and fantasy books, spanning every subgenre on the inkpapery.icu: Joel Cunningham. Bestselling authors and celebrities share their favourite books of the year, including fiction, poetry, cookbooks and more Best books of The best science fiction and fantasy of Dec 22, · If the quality of science fiction books released in is anything to go by, then the genre is alive and kicking.
We’ve seen plenty of great science fiction books releasing this year, some. The book options included a novella about an out-of-this-world college student, a tale about life in the sunken New York City of the future, and astronaut Scott Kelly's memoir about his year in inkpapery.icu: Hilary Brueck.
From women codebreakers during WWII, to Oliver Sacks on consciousness, to a graphic novel about theoretical physics, there’s something for everyone on this year’s list of best science books. Maria Popova, founder of Brain Pickings, and Deborah Blum, director of the Knight Science Journalism program, join Ira to wrap up the best science.
Apr 04, · The Best Science Fiction of the Year – Volume 2. Night Shade Books – April 4, ISBN ISBN The second volume in a new year’s best series.
NPR's Book Concierge is your guide to 's best reads. Use our tags to filter books and find the perfect read for yourself or someone you love. Visit the #bookconcierge, @NPRBooks' guide to. Dec 11, · Murder mysteries, family secrets and the end of the world -- the best fiction of features dark tales and moments of beauty.
The Best Science Fiction and Fantasy of the The latest in a series that has been called “a must-read for fans of science fiction, fantasy, and short stories in general”Science fiction is a portal that opens doors onto futures too rich and strange to imagine.3/5.
Latest Reviews Science io9 Field Guide Earther Design Paleofuture. Books. The Very Best Science Fiction And Fantasy Books Of You may also This has been a really great year for science. The Best Science Fiction of the Year 7 The Best Science Fiction of the Year #6 is an anthology of science fiction short stories edited by Terry Carr, the sixth volume in a series of sixteen.
It was first published in paperback by Del Rey Books and in hardcover by Holt, Rinehart and Winston in July Author: edited by Terry Carr. The Best Science Fiction of the Year #8 is an anthology of science fiction short stories edited by Terry Carr, the eighth volume in a series of sixteen.
It was first published in paperback by Del Rey Books in Julyand in hardcover by the same publisher in conjunction with the Science Fiction Book Club in Genre: Science fiction. The big read The Economist’s books of the year.
$ HQ; £ An indispensable book for anyone trying to understand the birth and meaning of modern science fiction in America from.


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The Perini Navi Group has announced that Italian investors the Tabacchi family are to become one of its major shareholders. Formally announced at a press conference in Milan earlier today (May 10), the Tabacchis will hold a 49.9% share in the company, acquired through a €27 million capital injection via holding company Fenix.
The remaining 50.1% will remain under the control of Perini Navi founder Fabio Perini through holding company Faper Group, of which Perini is also the founder. Fabio Perini will now assume the role of honourary chairman of Perini Navi Group while Lamberto Tacoli, president of Nautica Italiana and former CEO of Ferretti-owned builder CRN, will become the Group’s new chairman and CEO.
Speaking at the press conference, Tacoli added that Edoardo Tabacchi would be assisting him as deputy chairman, alongside current Perini Navi chairman Fabio Boschi, and explained of his move, “I was not looking for a job, but was looking for a home.”
“It is fantastic to see two large families in command of a top Made in Italy brand,” said Edoardo Tabacchi, who formerly owned eyewear giant Salmoiraghi & Vigano. A passionate yacht enthusiast, Tabacchi is also the former owner of 60 metre CRN Blue Eyes and spoke about his love of yachting with fellow owners Brian O’Sullivan, Alexander Rosa-Alscher and Paola Trifiro at 2016’s Superyacht Design Symposium.

domain: economics


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Tucker Carlson – he used to wear a bow tie and is Ivy league educated….BUT he is a self-righteously angry Hero of the working class and CARES about people just like you!
2. Rush Limbaugh – the man who started it all – who led OUR revolution against the real ENEMY – our own democratically-elected government. You need to get angrier and pay MORE attention to Rush – because the more attention you give him – the richer the gets. Thank you LOYAL listeners and attention-givers.
3. Ted Cruz – while his name sounds suspicious/Hispanic – he is one of US. You can tell by his smile that he is always telling THE TRUTH and not just what you want to hear so that Ted gains your trust, your vote, and your attention. TRUST TED!
4. Adolf Hitler – He gets such a bad name these days among the “mainstream media” and the multi-racial liberal animals but he recovered from a career as a failed actor before he successfully enflamed the humiliation of the German people in the 1930s and worked to restore tradition and greatness – to Make Germany Great Again. Unfortunately, it didn’t last long and the pure Nazis were defeated by the LIBERAL armies of the United Kingdom and the United States.
Now, we REAL Americans, call upon the realized Ubermench (the Superman) of pure white greatness to lead us in our campaign against the GLOBALISTS (i.e., George Soros) and the Liberal Media (i.e., the lying press)!!

domain: politics


Sometimes finding the right choice seems agonizingly difficult. Most people would probably agree without too much controversy that, all other things being equal, the extinction of species is not to anybody’s advantage, and that we humans ought to do our best to presevere orang-u-tans and tigers and whales and polar bears, and the hundreds of other mammals, fish, and most other living things that are currently under threat as a result of the inexorable onslaught of human activity.
But sometimes the right choice does not seem that obvious. Should, for instance, Montana ranchers and their domestic cattle take precedence over the last wild herd of American bison which have fought back from the brink of extinction a century ago? The farmers say their way of life will be destroyed if the bison roaming in Yellowstone National park are not culled drastically. Or should the Innuit people who have been living in Alaska for thousands of years be forbidden to kill the threatened polar bears and seals which are an essential food source for them if they are to continue to live on their native lands?
Should the way of life of Montana farmers be weighed in equal balance with the ancient way of life of the Innuit? Is it equally important to save the habitat of bison and polar bears? Along with the ethical dimensions of questions like these are the practical possibilities. The northern ice cap may be melting too fast to save either the polar bear or the Innuit way of life. The political fall out of culling thousands of bison may or may not be more important than the fall out of costing Montana ranchers millions of dollars in lost profits from encroaching bison and diseases ranchers say the bison are spreading.
There are hundreds more choices throughout the world like these. Almost certainly many times the outcomes of these choices have been and will continue to be tragic. But human ingenuity has found workable compromises as well. Africans who have found that there is greater benefit in the tourism drawn to see their wild animals than there is in slaughtering them fight against poaching. Companies who have found medical breakthroughs among threatened plants and animals are working to preserve them.
It isn’t always necessary to choose between who shall live and who shall die. Sometimes everybody living is a better option for us all.


Hello 5G Experts,
For NSA n/w, I have an LTE anchor site MCG in 2.3 GHz band and 5G NR mmwave SCG in the 38Ghz. band.
In the rrc reconfiguration, I can see the PDCP entity allocated in 5G but there is no 5G mmwave coverage at the Cell edge as expected and no B1 event for SCG addition in Phy layer,.
So the Flow goes like 5G PDCP>4G RLC-MAC> 4G PDSCH
And in the cell center I have good 5G mmwave coverage and so the flow does like
5G PDCP>5G RLC-MAC> 5G PDSCH.
My question is, what is the criteria for allocating 5G NR PDCP entity ?
What I observe is that PDCP entity allocation does not have a dependency on 5G coverage presence. Is it a default 5G PDCP allocation for all LTE anchor sites with X2 to gNB ?


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The wonders of Bukhour and its impact on the Arab World
March 26, 2017
This presentation explores the science behind scents in the Middle East in general, and “Bukhur” in specific. It focuses on its economy, culture, and chemistry.Coming Soon!
Sources & Future references
- Mecca Trade, Spices of Araby, Classical Spice Trade by Patricia Crone.
- Perfumes and Cosmetics in the Ancient World Dayagi-Mendels, Michal, 1993.
- Inside Arabia Felix
- The Ancient Perfume Route
- The Story of Frankincense
If you would like to contact the presenter directly, please email us at afikra and we will connect you!

domain: library


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Just a few months ago I wrote a short essay and entered it in 20/20’s contest for a guest editor-in-chief. Much like Jack and his infamous magic beans, others scoffed at my wishes of winning and thought my hopes were as thin as the clouds in the sky. But I had a small hunch that my little essay might grow into something big and sure enough… I won! And I soon found myself staring straight out of my window at a large and wondrous beanstalk (which in my case was more like the Holland Tunnel). It led me straight to the Soho offices of 20/20, a realm I had never dreamed I’d see.
I found myself climbing to new heights, relishing challenges and breathing the sweet air of experiences I had never known. I edited copy and reviewed layout pages while enjoying my own Manhattan office with a view. I interviewed the talented Lisa Loeb and watched her perform live on stage that very same night at EyeRock. I live tweeted from a fashion photo shoot showcasing glamorous models in eyewear. And did I mention I did it all while being housed in fabulous accommodations in the heart of Times Square?
Yes, I landed a posh job with the ultimate perks but the real job of James Spina, 20/20’s actual Editor-in-Chief is certainly nothing to bat an eye at, for his shoes could never be filled in the blink of an eye. He and the staff at 20/20 are hardworking, resourceful and determined while still being artistic, imaginative and fun. They may have their heads in the eyewear clouds but their feet are still planted firmly on the ground. They have been a huge inspiration to me, and one day I hope I can return to 20/20. I have a feeling the journey has just begun.
Cheryl G. Murphy

domain: artistic


The industry I know finest is automotive, and it’s an trade plagued by externalities. Yates Automotive Inc. in Alexandria, VA gives a full vary of automotive service and repairs together with oil changes, brake service, tune-ups, scheduled upkeep, and alignments. Along with the free content material made accessible on the sites an intelligence store will provide you with premium market evaluation reports from the main international suppliers of market research and business evaluation.
Our magazine provides excellent platform for companies, advertisers and media companions who deserve the attention of Africa’s leading enterprise executives, to showcase your companies and place you within the arms of the continent’s most influential individuals.
Modern software developments could make tomorrow’s automobiles exceptionally costly: OEMs and suppliers must earmark resources for buying new know-how and recruiting experienced technical talent. Many of the new options going into cars require the expertise of software program engineers, who by and large desire the ostensibly more dynamic work environments of Silicon Valley startups to these of the automotive trade. In consequence, a number of the latest mergers and acquisitions within the car sector have been undertaken to augment in-house technical information and capabilities. For instance, German supplier ZF Group, which paid US$12.4 billion in 2015 to amass TRW with a purpose to expand into the digital safety and connectivity market, took a 40 percent stake in vehicle radar provider Ibeo Automotive Methods in 2016.
Interestingly, most employment in this trade is upstream of the original equipment manufacturers (OEMs) since Klier and Rubenstein emphasize that carmakers increasingly deal with ultimate meeting having largely handed on the accountability of producing the bulk of their auto parts to impartial suppliers. 3 Figure 2 summarizes the proportion of each state’s automotive employment that works for automotive parts manufacturers (NAICS 3363) and we see they’re … Read More...


Much of the commentary concerning the presence of classified documents at the Penn Biden Center office and the President’s Delaware home has focused on the delay between the discovery and the public revelation of that discovery. And it is difficult to believe that the timing was apolitical. There is another question about the timing, however, that no one seems to have asked: Why were classified documents that had been in Biden’s possession since 2017 suddenly found less than 90 days after the FBI raid on former President Trump’s Mar-a-Lago compound?
That raid was obviously a well-orchestrated piece of theater calculated to draw maximum coverage by the corporate news media and to be seen by the American public. Most objective observers assumed this was just another phase of the perpetual “get Trump” project that has obsessed the Democrats since he defeated Hillary Clinton in 2016. There is another potential explanation for the Mar-a-Lago raid that no one has posited, probably because it conjures up images of paranoics in tinfoil hats. However, if the Russia collusion hoax taught us nothing else, it is that the Democrats are by no means above concocting real conspiracies.
Biden’s people all but certainly learned long before Nov. 2 or Aug. 8 of last year that he was illegally in possession of classified material — and had been stunningly sloppy about security. These people are smart enough to know Biden’s classified document problem would eventually become public knowledge, and that damage control would be necessary. What better way to preemptively indemnify the Big Guy than to raid the home of former President Trump on the pretext that he was committing a crime of which Biden himself is guilty? But this may very well backfire, as former Assistant Secretary of State Robert Charles told Fox News:
The bigger thing is the nature of the documents. We know in this case, unlike in Trump’s, that these are potentially very incriminating documents. They relate to Ukraine. The president’s son was taking millions of dollars from Ukraine and from China. They’re housed in a place that China helped to fund. There’s some really questionable things here and I guess the last big question that has to be answered is did Hunter Biden, who was on the pay of Ukraine and China, we know this, did he have access to these documents, and if he did, why, and what does that mean?
As to whether Hunter Biden had access to some of the classified documents, the answer is clearly, “Yes.” It has been widely reported that the classified material has been found in at least three locations — the Penn Biden Center, Biden’s Delaware residence, and the garage where his 1967 Corvette Stingray is housed. The Washington Free Beacon reports, “The home where President Joe Biden improperly stored a number of classified documents was also Hunter Biden’s residency for a number of years.” The New York Post’s Miranda Devine tweeted a document on which Hunter lists his address as the Biden house in Delaware.
All of which suggests that the hopelessly slapdash manner in which Biden stored classified documents involves serious national security implications. President Biden, however, seems to be having trouble grasping that reality. Peter Doocy of Fox News questioned the President about the propriety of storing classified documents next to his Corvette. Doocy literally asked Biden, “What were you thinking?” The latter snapped, “My Corvette is in a locked garage, OK? So, it’s not like they’re sitting out on the street.” Meanwhile, Breitbart News reports that that National Archives and Records Administration (NARA) is stonewalling Congress:
On Wednesday, Rep. James Comer, the chair of the House Oversight Committee, requested the National Archives disclose “all documents and communications between or among the White House and the Department of Justice or NARA [National Archives and Records Administration] regarding the documents retrieved from President Biden’s personal office at the Penn Biden Center.” The National Archives has apparently not complied. Comer told Fox News on Saturday the National Archives is stonewalling the Oversight Committee’s investigation.
When the FBI raided Trump’s Mar-a-Lago compound, on the other hand, his lawyers were negotiating with NARA about which documents Trump could keep and which ones should go back to D.C. The Trump material was housed in a vault (not a closet or a garage) inside a walled compound crawling with Secret Service agents. Yet, at dawn on Aug. 8, 2022, the FBI suddenly descended on Mar-a-Lago as if they were raiding the headquarters of Scarface. They threw his staff out of the building and ransacked the place while agents with automatic weapons stood guard at every entrance as if they expected a counter-offensive.
In the end, it’s probable that the Mar-a-Lago raid was just a preemptive exercise in damage control designed to make “Car-a-Lago” appear more innocuous than it really is. Its purpose was to indemnify Biden against serious consequences from his own misuse of classified material. This suggestion will be easy to write off by the usual suspects as a conspiracy theory, but Biden and the Democrats have been caught concocting more than one genuine conspiracy. That is the lesson of the Russia collusion hoax. As to the special counsels assigned to each case, it couldn’t be more obvious that Jack Smith and Robert Hur are insurance agents.


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Wed, 14 September 2016
-----SUPPORT THE SHOW-----
Click here to make a contribution to the podcast --> http://www.globaldispatchespodcast.com/support-the-show/
World leaders gather at the annual United Nations General Assembly in New York next week. There will be much political drama and diplomatic storylines that I'll discuss in a later episode. But behind all the politics and drama are issues of substance -- and arguably the most important substantive issues on the table relate to the global refugee crisis.
There will be two high profile summits at the UN related to refugees. The first is organized by the United Nations itself, called the "Summit for Refugees and Migrants." The second is being organized by President Obama and is the "Leaders Summit on Refugees."
Taken together, these two high level meetings at the UN have the potential to provide an important inflection point in the international community's attempt to address the largest global displacement crisis since World War Two.
On the line to help me to help put these two summits in a broader context of how countries confront a growing refugee crisis and an ever increasing number of migrants around the world is Shannon Scribner, a humanitarian policy adviser for Oxfam. Shannon describes what these two distinct summits hope to accomplish, some of their benefits and weak points, and explains the exceedingly complex challenge of crafting a global strategy to confront this global problem.
Direct download: Refugees_and_Migrants_-_Global_Dispatches.mp3
Category:general -- posted at: 6:28pm EST

domain: news


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central park printable map girls trip and city show me of new york.
map of us states and cities maps united major with cool capitals to important random 2 full usa.
to high speed rail alternatives texas road map county lines.
download free printable map of north carolina counties.
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the blog hong kong in world map political.
new and map of vermont hampshire towns.
national parks map of california showing yosemite park.
capitals of the world printable map with continents and countries.

domain: geography