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ABC may want to get with John Travolta about a “Boy in the Plastic Bubble” SEQUEL. Because it could have a very happy ending now . . .
Researchers at St. Jude Children’s Hospital in Memphis just announced a new treatment that could totally CURE his character.
The disease he had in the movie is called severe combined immunodeficiency, or SCID for short. And it’s when you’re born without an immune system. Without treatment, the survival rate is only about a year or two.
The new treatment involves harvesting stem cells from the patient’s bone marrow, then modifying their genes. And researchers use a deactivated version of HIV to do it.
A similar treatment made headlines five years ago. But apparently this one’s even better, because it fixes all three types of cells needed for a healthy immune system.
The president of St. Jude’s says it works so well, they’re comfortable using the word “cure.” The only question is whether more treatments will be necessary later on.
So far, once is enough. And kids who get the treatment end up with full immune systems, just like the rest of us have. Now they just want to see if the kids still have all that immunity 10 and 20 years down the line.


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Have you been tasked with organising the works Christmas party? You can book either our great value ‘Festive Set Menu’ starting from just £21.95 per person, or our ‘Festive Buffet Menu’ at £14.95 per person, just to guarantee your party goes off without a hitch! Our menus have been designed with everyone in mind, and we have a full selection of gluten free and vegetarian options available. Simply click the links above to view our menus, and then send us an enquiry via the ‘Book Now’ button.
Also, why not take the hassle out of Christmas Day and join us here at The King’s Head for five fabulously indulgent courses. Our freshly prepared Christmas Day lunch menu features mouth-watering starters such as, sesame halloumi bites and beef fillet carpaccio with horseradish rosti, followed by one of our delicious mains, including traditional roast rolled turkey breast with cranberry, sage & roast garlic stuffing, braised goose leg, goose fat roast potatoes & pigs in blankets, or beef or halibut. As well as all of this you can then finish your meal with one of our unforgettable desserts.
This year we are also offering, with only a £15 supplement, an arrival glass of fizz and dessert cocktail to make your meal even more special!
So, whether it’s an intimate celebration for two, a corporate party for 200, a sit down family meal, buffet, or Christmas Dinner, we can cater to your needs, and make sure you get to enjoy all of the festivities too!
Get in touch with our Reservations Team on 0121 277 4130 to find out more.

domain: food


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Emerging Therapies in Relapsing-Remitting Multiple Sclerosis
James J. Marriott* and Paul W. Q'Connor Reviews on Recent
Clinical Trials, 2010, 5, 179-188.
Disease modifying therapy (DMT) first became available for relapsing-remitting multiple sclerosis (RRMS) fifteen years ago with the development of the moderately effective injectable agents interferon (IFN)-~ and glatiramer acetate (GA). The subsequent licensure of mitoxantrone (MX) and natalizumab (NZ) has allowed for better control of refractory disease at the expense of potentially life-threatening side effects in a minority of patients. This dichotomy between DMT potency and safety also characterizes the next generation ofDMTs.
Five oral medications (fingolimod, cladribine, teriflunomide, laquinimod and fumarate) are at various stages of phase lll clinical trials and it is anticipated that at least some of these will be on the market within the next year. The development of oral agents would be a tremendous advance with respect to convenience and it is anticipated that this would dramatically increase the number of patients on therapy. In parallel with oral therapies, powerful immunosuppressive monoclonal antibodies alemtuzumab, rituximab / ocrelizumab, daclizumab) are also being evaluated.
Enthusiasm for the next generation of therapies is tempered by safety concerns. Serious and occasionally fatal complications have occurred with the emerging monoclonal therapies and rigorous patient selection will be required for these agents. Moreover, some of the oral DMTs that are most eagerly awaited by patients have also been associated with serious side-effects in the trials to date. It is unclear how oral agents will be incorporated into future treatment algorithms given the need to weigh the ease of oral administration against the relative inconvenience but long-term safety of current first line injectable therapies.
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domain: medical


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Jailer Box office collection Day 17: Movie crosses ₹300 crore mark in India, eyes ₹600 crore worldwide. Details here
Regaining its momentum, superstar Rajinikanth's movie earned ₹5.50 crore in India on third Saturday
Jailer Box office collection day 17: Superstar Rajinikanth's movie is back on track with a slight surge in its collection on third Saturday. Jailer movie earned ₹5.50 crore in India for all languages, according to Sacnilk.com.
The movie is expected to cross ₹600 crore earning worldwide in the next two-three days.
About Jailer movie
The movie is directed by Nelson Dilipkumar and made under the production banner of Sun Pictures. The movie stars Rajinikanth, Tamanna Bhatia, Ramya Krishnan, Mohanlal, Jackie Shroff, Shivarajkumar, Sunil Varma, Yogi Babu, and Vasanth Ravi in key roles.
The movie had initially released with a collection of ₹48.35 crore nett in all languages on August 10. The film experienced a substantial surge in collections in second week. During that week, its accumulation surged to ₹16.25 crore nett on Saturday. During the opening week, ‘Jailer’ collected ₹235.85 crore nett in India across all languages. This Tamil film has been dubbed in Hindi, Telugu, Malayalam, and Kannada.
The movie also features Shiva Rajkumar, Mohanlal, and Jackie Shroff. Its music is composed by Anirudh Ravichander.
Milestone Alert!Livemint tops charts as the fastest growing news website in the world 🌏 Click here to know more.

domain: news


There’s an email going around Bonney Lake and a flurry of Facebook activity regarding a white SUV that’s been spotted trying to snatch kids.
Before I could get all the words out of my mouth, a record specialist at the Bonney Lake Police Department told me the report is a rumor, completely false.
“It is inaccurate,” she said.
A coworker sent me this Facebook post this morning from a friend of hers:
A White SUV with nice rims and a skull trailer hitch cover trying to snatch kids in BonneyLake. At the park in Brookside this car had an adult jump out and grab one of the kids, luckily a parent was close enough to scare them away. Police were called and police said its the 4th incident with this car! Keep your eyes out friends in BL call police if you see this vechile!
In addition to the Facebook activity, there’s an email with the same information. Bonney Lake police have been receiving several phone calls a day about the activity.
There are no reports of such activity in Bonney Lake or with the Pierce County Sheriff’s Department.


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Hyderabad :Martina Hingis, Flavia Pennetta, Jelena Jankovic, Thomas Johansson and other top tennis players would cross swords when the Champions Tennis League (CTL) 2015 begins on November 23.
After a successful inaugural edition last year, the Vijay Amritraj-promoted tournament would begin in Mumbai and it will see 13 matches being played over a two-week period.
The event would conclude with a grand finale in Hyderabad on December six, Amritraj told reporters today.
CTL features six city-based teams across the country, put into two zones, he said.
“Each zone has three teams, where all teams play each other in a home and away format. The team winning the highest number of games in their respective zone will play each other in the grand finale to win the prize money of Rs one crore. The runner-up would win Rs 50 lakhs,” he said.
The six teams include Punjab Marshalls, Mumbai Tennis Masters, Raipur Rangers, Nagpur Orangers, Hyderabad Aces and an yet to be announced sixth team.
“Over the years, I always said that we only had the Chennai Open as the international tennis event in India. Champions Tennis League brings international tennis, world class tennis to different cities across India. We have Indian Davis Cup players who are part of each team to participate alongside great champions.
“And, we will also have two juniors in each team, one boy and one girl, to learn and experience what it is to be alongside these great champions,” Amritraj said.
The Hyderabad Aces comprises Hingis, Ivo Karlovic, Thomas Johansson, Jeevan Neduncheziyan and the two juniors Sathwika Sama and Adil Kalyanpur.
Amritraj said the CTL is delighted to have Hingis, who has been having a great run for some time now in the company of India’s star player Sania Mirza in the doubles category in international tennis.
Replying to a query, he said Indian players are doing well in doubles and that things need to improve in others.
“Most of our performance has come in doubles. It is critical for us in men’s tennis to make the world group in Davis cup, top 16 teams, that you have to have players in the top 100, hopefully in the top 50, to make the world group. If you do not have players in top 50, top 35, you are going to struggle to make the world group.
“Because everyone there is in the top 20, top 25. So it is difficult unless you do that. Similarly, in the Fed Cup as well amongst women,” he said.
Asked about India’s medal prospects in the Rio Olympics, he said tennis is a “non-seasonal sport” that is played almost throughout the year and that the players mainly look forward to the next Grand Slam event.
The tennis players would not look forward to the Olympics like their counterparts in athletics would do, he said.


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The National Basketball Association (NBA) yesterday announced that three Nigerian superstars, Grammy Award-winning singer, songwriter and record producer Burna Boy, 2023 Grammy Award-winning singer and Oscar-nominated singer and producer Tems and rapper and singer Rema, will headline the NBA All-Star Game 2023 halftime show with an Afrobeats-themed performance.
A statement issued by the NBA further revealed that after the performance, the NBA family will honour LeBron James for becoming the league’s all-time scoring leader.
The 72nd NBA All-Star Game will tip off at 8:30 p.m. ET (3:30 a.m. CAT) at Vivint Arena in Salt Lake City and air on beIN Sports, Canal+ and ESPN across the continent.
Prior to tip-off, global superstar Post Malone will perform a medley of hits following the first-ever in-arena NBA All-Star Draft and Vin Diesel, star of the upcoming “Fast X” movie, will welcome fans to the evening.
Payson, Utah, native and Grammy Award-nominated artist Jewel will sing the U.S. national anthem and Juno Award-winning and platinum-selling artist, Jully Black, will sing the Canadian national anthem on Sunday for the NBA All-Star Game.
Utah singing group The Bonner Family will perform the U.S. national anthem before the 2023 Jordan Rising Stars, which will be played on Friday, Feb. 17.
As previously announced, the group will also perform the U.S. national anthem and the Black national anthem, “Lift Every Voice,” at the NBA HBCU Classic presented by AT&T, which will tip off on Saturday, Feb. 18 at 4 p.m. ET on TNT, ESPN2 and NBA TV.

domain: sports


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Danish money transfers
Send money to Denmark with Currency Index - it's fast, easy and secure, and you can save up to 4% on the exchange rate alone, compared to your bank or other providers. Contact us using the form below if you would like more information on our service, or you can register with us to set up a trading facility today.
Currencies usually accepted: Danish Krone (DKK) or Euro (EUR)
Typical money transfer time to Norway: next day (Krone) or same day (Euro)
Transfer charge: FREE if sending over £30,000, otherwise £12 per TT payment (Krone or same-day Euro) or £7 per SEPA payment (next-day Euro)
Currency Index is able to transfer Krone or Euros depending on your requirements, or can help you work out which is the cheaper payment option when you need to send money to Denmark.

domain: economics


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MSM (METHYLSULFONYLMETHANE), A NATURAL SULPHUR COMPOUND APPEARS TO BE THE IDEAL COMPANION FOR GLUCOSAMINE & CHONDROITIN FOR EFFECTIVE RELIEF OF OSTEOARTHRITIS.
Tomorrow is World Arthritis Day. Afflicting millions worldwide, arthritis is a chronic degenerative disease that can vary in severity. With its widespread prevalence and effect on health and well being, the disease accounts for a significant percentage of doctor visits.
Arthritis is defined as the inflammation of one or more joints and symptoms of the condition include redness, swelling, tenderness, stiffness, pain, and warmth in the affected area. There are over 100 types of arthritis, including osteoarthritis, rheumatoid arthritis, ankylosing spondylitis, and gout.
Osteoarthritis (OA) is the most common type of arthritis. It is a chronic condition characterised by the breakdown of the joint’s cartilage.
Cartilage is the part of the joint that cushions the ends of the bones and allows easy movement of joints. Healthy cartilage is extremely strong and flexible and allows the joints to move smoothly and painlessly, but in osteoarthritis, the cartilage is damaged, and subsequently wears down. This process continues until little or no cartilage is left in the affected joint, causing bone to grind against bone.
Osteoarthritis typically affects certain joints, such as the hips, hands, knees, lower back, and neck. It occurs more frequently as we age. Before age 45, osteoarthritis occurs more frequently in males. After age 55 years, it occurs more frequently in females.
One needs to supply essential nutrients for the body to build strong, flexible cartilage. Having a strong flexible cartilage is the first step for healthy joints. Hence, with the right nutritional support, osteoarthritis sufferers can rebuilt and regrow their worn out cartilage.
Glucosamine & chondroitin are two important nutritional compounds used in the treatment of osteoarthritis.
Both Glucosamine & chondroitin are natural components of healthy joint tissue. They work by supplying the natural raw ingredients cartilage needs to repair and rebuild itself. They also suppress the natural enzymes that break down cartilage in the first place.
- Glucosamine is a natural compound that is found in healthy cartilage. It is a normal constituent of glycosaminoglycans in the cartilage matrix and synovial fluid. It is used in the formation and repair of cartilage.
- Chondroitin is the most abundant glycosaminoglycan in cartilage and is responsible for the resiliency of cartilage. It acts as a “water magnet”. The ability of chondroitin to hold water gives rigidity to the cartilage and also enables it to act as a “shock absorber”.
Supplementation with Glucosamine & chondroitin helps to relieve joint pains, rebuild cartilage, improve flexibility, lubricate joints, promote healthy connective tissues, and reduce stiffness.
In recent years, studies have shown that two-in-one Glucosamine & chondroitin supplements are far more effective in treating moderate-to-severe osteoarthritis than potent anti-inflammatory drugs (celecoxib) or even glucosamine or chondroitin taken separately by itself.
MSM (Methylsulfonylmethane), a natural sulphur compound with many health benefits, is widely used together in combination with Glucosamine & chondroitin for effective relief of joint pains. Many developed nations across the globe such as the US, UK, Europe, Australia, etc regularly observe very positive results in the treatment of osteoarthritis when oral MSM supplements are added to Glucosamine & chondroitin nutritional therapy.
MSM is found in the normal diets of humans and almost all other animals. MSM is made up of 34% sulphur, the fourth most abundant mineral in the human body. Sulphur is necessary for the structure of every cell in the body. It is needed for the structural integrity of joint cartilage and connective tissue such as skin, hair, and nails.
Many amino acids, the building blocks of protein, have sulphur as a component. Hormones, enzymes, antibodies, and antioxidants all depend on it. And because the body utilises and expends it on a daily basis, sulphur must be continually replenished for optimal nutrition and health.
The Arthritis Foundation of America recommends starting with a low dosage of 500mg MSM twice a day and increasing gradually to 1,000mg MSM twice a day.
Lifestyle tips for osteoarthritis sufferers
- Physical activity keeps joints flexible and maintains or improves muscle strength.
- Weight control is important for the prevention and slowing down of the progression of OA affecting weight-bearing joints (knees and hips) and the lower back.
- Weight loss of only 15 pounds can reduce knee pain for overweight individuals with arthritis.

domain: health


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Level up your home decor with Hallowed Solar Table Lamp!
The mild mood lighting provides a lovely and relaxing environment. From a gazebo, beneath an umbrella, the perfect garden décor
Built from high-quality metal with a rustproof finish and corrosion resistance, they’re also heatproof, waterproof, and ready for whatever Mother Nature throws at them.
Solar garden lights give out a mellow yellow glow at night and create a beautiful pattern on a table or the ground. casting a lovely, whimsical pattern of cheerful clouds
Item Weight 13.1 ounces
Package Dimensions 6.57 x 4.8 x 4.69 inches
Batteries 1 AA batteries required. (included)
Style Art Deco
Color Warm White
Shade Material Metal
Power Source Solar Powered
Switch Style Touch
Batteries Included? Yes
Type of Bulb LED
%100 HIGH QUALITY
Uniquely glamorous and authentic Hallowed Solar Table Lamp handcrafted with love and passion in our ateliers in Morocco by housewive artisans with extraordinary quality and checked within the UK, suitable for any decor and style.
Our lamps are handmade and are sent in original packaging to provide safe and secure delivery.
ROMANTIC & SOOTHING
Create a soothing and romantic atmosphere with the authentic effect of the Hallowed Solar Table Lamp.
For more Moroccan Lamps click HERE!

domain: artistic


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SpaceX is about to send another group of astronauts—three from NASA and one from the European Space Agency(ESA)— to the International Space Station (ISS) in the world’s only reusable orbital rocket.
A Falcon 9 will launch a brand new and unused Crew Dragon capsule called “Freedom” containing four “Expedition 68” astronauts this week when SpaceX and NASA finally launches the Crew-4 mission on an early morning mission.
It’s been delayed because the Axiom Mission 1 (Ax-1)—the first all private astronaut mission to the ISS—had to delay its undocking and splashdown due to bad weather. However, they’re now home safely.
It will be SpaceX’s fifth flight with astronauts as part of NASA’s Commercial Crew Program. The other private company in that program is Boeing, whose Starliner spacecraft requires another test flight in May prior to a planned crewed mission to the ISS in September.
When is the NASA SpaceX Crew-4 launch?
NASA and SpaceX are targeting Wednesday, April 27. The launch will take place at Launch Complex 39A at the Kennedy Space Center, Florida.
The launch is targeted for no earlier than 3:52 a.m. EDT (7:52 a.m. UTC).
Crew-4 has another launch opportunity available on Thursday, April 28, said NASA.
Where and when to watch the NASA SpaceX Crew-4 launch
NASA Television mission commentary coverage begins at midnight EDT.
At around 05:30 a.m. NASA Television will broadcast a post-launch news conference.
“Freedom” is scheduled to dock to the space station at 8:15 p.m. EDT on Wednesday, April 27.
Who will be on the NASA SpaceX Crew-4 mission?
Inside the SpaceX Crew Dragon spacecraft will be NASA astronauts Jessica Watkin (mission specialist), Bob Hines (pilot) and Kjell Lindgren (commander) as well as European Space Agency astronaut Samantha Cristoforetti (mission specialist) of Italy.
Cristoforetti visited the ISS in late 2014 for six months and was succeeded by Lindgren in 2015.
Expedition 68 will spend 180 days in space.
When will the NASA SpaceX Crew-4 launch reach the ISS?
Crew-4 will have a 24-hour journey to the ISS.
Docking is scheduled for 6 a.m. EDT on Sunday, April 24, with the hatch being opened at 7:50 a.m. EDT.
Wishing you clear skies and wide eyes.


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Western’s non-tenure track faculty may have fewer jobs available to them, and thus lesser income in 2020-21 than previously planned, according to a faculty email from the dean of Western’s College of Humanities and Social Sciences, Dr. Maria Paredes Mendez on Thursday, July
Western Communications Director Paul Cocke said he was unaware if other emails similar to Paredes Mendez’s were issued to other department faculties.
Non-tenure track faculty at Western include senior instructors, adjunct faculty – professors who work on a contractual basis and usually are part-time – and teaching assistants. Unlike tenured and tenure-track professors, non-tenure track faculty are not protected by long-term employment agreements and their labor is cheaper than tenure-track faculty, with fewer benefits in many cases.
Non-tenure track staff made up about 42% of Western’s faculty in 2019-20, according to data available from Western’s Office of Institutional Effectiveness..
“I have heard that non-tenure track faculty have been put in some sticky situations in terms of cutbacks, but I don’t have a lot of details on that,” said a Western teaching assistant who requested to remain unnamed to keep their position from being jeopardized. “So far I have still been granted the same hours that I would have in the past as a TA, and I believe they have been working hard to maintain this agreement.”
They said the availability of their advisers has been heavily affected and that interaction with them is critical during their two-year program as a teaching assistant. The lack of availability combined with professors’ lack of time to adjust to remote learning has had a negative effect on students’ learning, they said.
The reductions and contract uncertainties are due to Western’s incoming first-year class being 12% smaller at commitment date than last year’s, according to Paredes Mendez’s email.
Paredes Mendez said that because the campus experience will look different due to COVID-19, first-year students who have committed may decide not to attend as fall approaches, pushing that figure up further.
“What is expected is that more students than usual will decide to maybe take a gap year or go to school closer to home if they have to do it online anyway,” Paredes Mendez said. “What I heard was that the 12% reduction in the freshman class at the commitment date may be more like a 15% reduction by the time fall quarter starts.”
Western is already facing a possible 15% reduction in state funding, which makes up half of the university’s budget, according to Western’s Continuation Operating Budget. Housing revenue is also expected to continue to decrease, with only one student assigned to each room in the fall to mitigate the spread of COVID-19.
The degree to which tuition revenue will be affected is still unclear even on a preliminary basis because of the many fluctuating factors, Cocke said.
“[Revenue lost] depends on the student mix — resident vs. non-resident, undergraduate vs. graduate — and since enrollment numbers are changing daily, it is difficult to give a hard number at this point,” Cocke said.
Paredes Mendez said in the email that any non-tenure track contract reductions would come in the form of faculty not being assigned to all of the courses they agreed upon in January and February. This would result in the schedule and number of available courses being slightly reduced.
“We are revising schedules at this point based on current and projected enrollment, which is changing daily,” Cocke said.
Cocke said that large reductions are not anticipated, with most courses still planned to be available, and that Western’s main concern is retaining the courses needed for students to complete degrees and graduate on time.
Cocke said the departments with the largest body of non-tenure track faculty are English, math, and modern and classical languages. A full database of faculty type per university department is also available via Western’s Office of Institutional Effectiveness.
This, however, does not mean these will be the departments with the largest reductions, Paredes Mendez said.
“It really was a decision based not on the number of NTT [non-tenure track] faculty that a given department has, but rather what the scheduling in that department looked like, what the enrollment at this point looked like,” Paredes said. “Whether it was very similar to the past or not, whether there was room that there could be a few reductions without impacting students being able to take GUR’s and graduating on time.”
Paredes Mendez said that the irregularity of the pace and timeline of events from COVID-19 has made the certainty of the information available much more difficult.
“This year everything became sort of unraveled once COVID-19 happened and we began realizing what the potential implications of that were,” Paredes Mendez said. “It was very unfortunate and it was very difficult, but particularly for the [non-tenure track faculty] waiting to hear ‘Is my job going to look like what I thought it was going to look like?’”
Kameron Decker Harris — a Washington Research Foundation postdoctoral fellow from the University of Washington dually-appointed in both computer science and engineering, as well as biology — is beginning at Western in the fall.
“My tenure track job is still going ahead as planned,” Harris said. “The only difference from usual is that my classes will be entirely online this fall.”
Non-tenure track faculty will have more certainty about their job safety when the contract deadline hits on Wednesday, July 15, Cocke said.
“Because of the fluidity of the situation, not all classes for winter and spring may be contracted at this time,” Cocke said. “But we are endeavoring to contract as many sections as possible under the circumstances.”


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Dr. PHILIP GETSINGER
Medford, Oregon Map
|Practice:||Dr. Philip Getsinger, DMD|
|Online Appt. Scheduling:||Unknown|
|Accepting New Patients:||Y|
Above profile data last updated: 10/10/13
Dr. PHILIP GETSINGER has an excellent overall rating on RateMDs.com.
New! - Questions and answers about this doctor
Dentist-related: cavities, gum disease, teeth cleaning, dental care, gingivitis
The following ratings and comments have not been substantiated by RateMDs.com.
Best dentist I've ever been to. I could tell Dr. Getsinger truly cared about me and my health
Paid (or co-pay): $200


New York’s Museum of Modern Art (MoMA) is planning to buy NFTs with the proceeds of the sale of William S Paley Foundation’s artworks price $70 Million.
As per stories, the museum intends to promote $70 million price of the gathering which had been beneath the care of the founding father of the CBS broadcasting community. Sotheby’s will unload 29 of Paley’s 81 items at MoMA later this 12 months.
The spectacular assortment contains Pablo Picasso’s 1919 “Guitar on a Table” and artworks by Renoir and Rodin.
It is price mentioning that Pablo Picasso’s 1919 Cubist “Guitar on a Table”, which as soon as adorned the area above the fireside in Mr. Paley’s Manhattan bed room, can be up on the market for at the least $20 million on 14 November in New York.
Yet one other historic piece, Francis Bacon’s 1963 triptych, “Three Studies for Portrait of Henrietta Moraes”, can be accessible for at the least $35 million on 14 October in London, revealed Sotheby’s.
MoMA’s director, Glenn Lowry, stated the museum needs to broaden the digital attain of the museum after a pandemic-stricken stoop within the variety of attendees.
The museum’s staff is keeping track of the digital artwork market and can be contemplating buying digital artwork related to NFTs. The staff may even presumably launch its personal streaming channel to doubtlessly buy extra artwork, digital or bodily.
Compiled by Coinbold


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The city of Durango and Axis Health System (AHS) are putting the finishing touches on a program that allows a Durango police officer and behavior health specialist to jointly respond to calls.
The model offers a more comprehensive approach to complex situations involving a behavioral health component. Co-responder teams respond to mental health calls that meet specific criteria with the goal of assessing individuals’ social needs and linking them to appropriate behavioral health resources.
“Our officers are spending more time helping people with social and emotional needs,” Chief of Police Bob Brammer said. “To respond differently, we need different capacity. This program provides that.”
A behavioral health specialist and law enforcement officer team are currently training as a team. AHS is hiring additional behavioral health professionals for the program, which is funded by the city and AHS. Co-responder teams will respond to calls within Durango city limits during certain days and times.
“We expect to go live with the program by the beginning of March,” Brammer said. “Until then, we are training and learning, making an effort and impacting the community as we grow.”
The Durango Police Department has worked closely with AHS and the 911 Communications Center in connecting with other agencies to learn about best practices for launching co-responder programs.
Co-responder programs in other parts of the U.S. and Colorado have demonstrated improved interactions between law enforcement and people they serve while helping reduce stigma associated with asking for help. Benefits include:
- More individuals connected to appropriate services instead of being arrested, put in mental health holds or emergency transports.
- Community members having more trust in law enforcement. Individuals who interact with co-responder teams are more likely to accept help and follow through on key referrals from the team.
- Improved referral capacity from 911 dispatch. Since co-responder teams cannot respond to every call, robust referral protocols based on field experience assist law enforcement in better assisting community members in need of behavioral health or other services.
- Connection to resources and follow-up. Once legal consequences are ruled out, co-responders can assist individuals in a variety of ways, including connections to resources, transport to a hospital or clinic or support for family members and others on-scene.
“We are incredibly excited to be partnering with the city and Durango police in offering this new pilot program in Durango,” Shelly Burke, CEO of Axis Health System said. “Nationally, there is a recognition that a portion of the calls for law enforcement response involve causes or symptoms that are outside of the expertise or resources of most police departments.”
“Community Mental Health Center staff increase the range of response options that can be offered when additional social or behavioral health services may be appropriate. We feel the co-responder program can build a better bridge between social and health needs that may be part of certain emergency 911 calls.”
For more information about AHS, visit AXIShealthsystem.org


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This vacation condo is situated in the Grand Bay area of Key Biscayne, an island six miles off the coast of Miami. It overlooks 1,200 feet of golden sand beach on the eastern shore. The hotel is just 20 minutes from Miami International Airport, and 30 minutes from Fort Lauderdale Airport. (Taxi, luxury coach, or limousine service is available. Arrangements must be made in advance.)
The guest room features an AM/FM clock radio with alarm (some come with a CD player), sumptuous marble baths, goosedown and non-allergenic foam pillows, plush terry bathrobes, luxurious 100% cotton linens, 300+ thread count, generously sized terry bath towels, multi-line telephones with a hold button, a generously sized desk, a remote control color television, barrier-free accessibility, a lighted makeup mirror, a hair dryer, scale, sewing kit, and suit, skirt, and padded hangers. The room features spectacular views of Biscayne Bay and tropical gardens, as well as city views of downtown Miami.
Additional amenities include an in-room safe, computer and fax hook-ups, high-speed Internet access with dataport, and a large private balcony.
Guest services include 24-hour room service, overnight laundry service, valet parking, a complimentary morning newspaper, an extensive on-site fitness center, babysitting services (available upon request), a business center, executive business services, and a kids' program.
There are two outdoor heated swimming pools, a whirlpool, and lush natural landscaping.
Specialty shops include a full-service beauty salon, customized catering, and gift and sundry shops.
The business services include a conference concierge, express check-in/departure, 24-hour secretarial services (fax, photocopies, telex, courier), a technology butler, and translation services.
Airport shuttle service is provided by the hotel or a local transportation company (fee may be applicable).
Dining and entertainment can be enjoyed at The Cafe and Terrace -- offering traditional cuisine, and The Pool Bar and Grill, The Lobby Lounge, and The Library Bar -- all offering cocktails and light fare cuisine.
The conference facilities include more than 20,000 square feet (6,096 square meters) of meeting and banquet facilities, including the 10,302 square-foot (3,140-square-meter) Ballroom, the 2,497 square-foot (761-square-meter) Junior Ballroom, a 2,882 square-foot (878-square-meter) multi-function room, six individual meeting rooms, and an extensive outdoor function space.
The fitness and recreation center includes an expansive 20,000 square-foot (6,096-square-meter) spa featuring a complete fitness and wellness facility, personal training, a sauna, steam, massage, facials, specialty spa services, and a full-service beauty salon.
Other activities include boating, windsurfing, parasailing, scuba diving, snorkeling, sailing, deep-sea fishing, and wave running.
The kids' program is an exclusive, supervised children's program designed specifically for our younger guests, ages 5 to 12. The program allows parents to play while children stay with our trained and certified counselors. Each counselor is certified in infant, child, and adult CPR, basic first aid, and water safety.
We are Tammy and Jeff Caress from Florida currently living in New York City. We are both Florida Gators and Miami Dophin and Heat, Giants, Knicks and Yankee Fans.
You can get away from it all in Key Biscayne and still be 20 minutes from South Beach, Lincoln Road or shopping at the Grove or Coral Gables. We also like the beach and tennis. The tennis is top. CLiff Drysdale. We also love to bike on the bicycle trails which run all along he Key.
This property is uniquely situated to feel like you are totally away from it all. It is both tranquil and serenene with an understated feeling of pampered elegance. There are plenty of activities.for the whole family to enjoy as well.
Golf: Area attractions include Crandon Park and Beach (five minutes away), Bill Baggs State Park -- offering nature trails and the historic Cape Florida Lighthouse (five minutes away), Crandon Marina -- for trips to the nearby reefs, sailing, and sport-fishing charters (five minutes away), Vizcaya Museum and Gardens -- a Renaissance-style villa and formal gardens built in 1916 (ten minutes away), South Beach -- a fabulous Art Deco District (20 minutes away), Fairchild Tropical Gardens -- offering guided tours and educational programs (30 minutes away), Village of Key Biscayne -- featuring a number of fine shops and boutiques in a quaint tropical setting (five minutes away), the Streets of Mayfair -- offering fine shops and boutiques, restaurants, and cinemas (20 minutes away), Coco Walk in Coconut Grove -- offering a wide selection of shops, restaurants, and movie theaters (20 minutes away), and the Shops at Bal Harbour -- offering over 100 upscale shops and department stores (30 min. away)
|Rate Period||Nightly||Weekend Night||Weekly||Monthly||Event||Minimum Stay|
|$350 per night high season late Dec - March
$300 per night April
$250 per night May
$170 per night June- Sept
$200 per night Oct-mid-Dec
Payment is usually accepted in the quoted currency (USD) unless the currency and the amount is specifically agreed in advance with the owner / advertiser.

domain: travel


text:
The imbalance between oxidants and antioxidants in cells often results in pathological processes and/or diseases. This delicate balance is achieved in part by antioxidant enzymes such as glutathione peroxidase, catalase, and superoxide dismutase (SOD), as well as by low-molecular-weight reductants such as glutathione. We evaluated the effect of thiol reagents on the proliferation of the Jurkat human T-cell leukemia-derived cell line. The cells show a multiphasic behavior when grown in the presence of thiols. Low concentrations of N-2-mercaptopropionyl glycine (0.03 mm) cause growth arrest, and intermediate concentrations (0.3–1.0 mm) induce apoptosis. Similarly, 1 mm N-acetylcysteine or glutathione induce apoptosis in more than 40% of the cells. Surprisingly, the cells grow well in higher concentrations (3–10 mm) of these reagents. Because the I58T variant of human SOD2 is thiol-sensitive, we measured SOD in Jurkat cells grown in the presence of thiol agents, observing markedly less SOD activity. In cell-free extracts, thiols quickly eliminated the SOD2 activity. Jurkat cells contain little SOD2 activity, with a different electrophoretic mobility from that of normal lymphocytes. Single-strand conformational polymorphism analysis of the Jurkat sod2 gene revealed a pattern different from the wild-type gene, suggesting a mutation in the sod2 gene. This was confirmed by cloning and sequencing the gene. Jurkat cells are heterozygous for a new mutation, L60F, in exon 3 of the mature protein. Our findings suggest a possible association between decreased SOD2 activity and malignant phenotype.
Redox status of cells can determine when cells rest, proliferate, or die. Healthy cells have a well-regulated redox balance. Superoxide anion can elicit proliferation of resting cells; higher concentrations, however, may induce apoptosis and necrosis (1). The roles of reducing agents with regard to quiescence, proliferation, and apoptosis are not well understood.
Thiol compounds acting as redox modulators may affect the proliferation rates of actively growing cells, such as cancer and transformed cells. The Jurkat T-cell leukemia cell line grows in culture without stimulation, as cancerous and transformed cells do. The redox status of transformed cells has been correlated with their content of antioxidants. Oberley and Oberley (2) and Sun (3) have found low levels of the mitochondrial manganese SOD23 in nearly all of the cancer cell lines studied, as well as in the primary malignant tissues. Furthermore, in childhood acute lymphoblastic leukemia, SOD levels have been reported to be about one-half those of normal cells (4, 5). These deficiencies are presumably associated with an increase in the oxidative status of the cell, but the mechanisms that account for the regulation of these important antioxidants enzymes in mammalian cells are still unknown.
Mutations in SODs have been associated with human diseases. Seventy-one different mutations in the cytoplasmic SOD1 have been correlated with amyotrophic lateral sclerosis (6). A specific mutation (R213G) in extracellular SOD3 that decreases binding affinity for extracellular surfaces is found in ∼7% of the population at large, but in ∼16% of patients with renal failure (7).
The roles of thiols such as NAC have been controversial with regard to cellular responses such as proliferation and apoptosis. NAC and GSH, presumably acting as antioxidants, have been used to rescue T-lymphocytes from apoptosis (8). However, later studies demonstrated that some thiol compounds could paradoxically elicit apoptosis by acting as pro-oxidants (9). To understand how changes in the redox status of cells affect their proliferative rates, we conducted experiments using the human T-ell leukemia cell line Jurkat. The cells were subjected to a shift in their redox status by treatment with thiol agents such as MPG, GSH, and NAC. We hypothesized that the redox status of lymphocytes or Jurkat cells would determine whether they proliferate or die by apoptosis or necrosis. Thus, a thiol-induced shift toward a more reducing environment in proliferating lymphocytes might be expected to stop growth and induce quiescence. In fact, the response of Jurkat cells to increasing concentrations of thiols proved to be far more complex.
MATERIALS AND METHODS
Medium and Reagents.
RPMI culture medium, MPG, GSH, NAC, and cytochrome c were purchased from Sigma, Co. FBS, and calcium-magnesium-free Hank’s buffer balanced salt solution (CMF-HBSS) were from Life Technologies, Inc. Xanthine oxidase was purified from milk as described by Waud et al. (10)
Cell Lines and Cell Cultures.
The human T-cell leukemia cell line Jurkat (clone E6–1) was purchased from the American Type Culture Collection. Jurkat cells were grown and maintained in RPMI 1640 supplemented with 10% heat-inactivated FBS. The cells were cultured at 37°C under 5% CO2 atmosphere and 100% humidity and subcultured every 4 days. Jurkat cells (7.5 × 104) were placed into 24-ell plates to a final volume of 1 ml/well under various experimental conditions. Growth was measured by direct counting with a hemocytometer. Viability was assessed by trypan blue exclusion.
HPBLs were purified using Ficoll gradients (Accu-Prep, Accurate Chemical, and Scientific Corp.). The blood was centrifuged for 20 min at 2200 rpm and the leukocyte “buffy coat” layer resuspended in 30 ml of Hank’s buffer. The cell suspension was underlaid carefully with 20 ml of Ficoll-Hypaque and centrifuged for 30 min at 1750 rpm. The layer containing the HPBLs at the interphase of the gradient was recovered and resuspended in Hank’s buffer to 40 ml. The cell suspension was centrifuged for 10 min at 800 rpm and the pellet washed twice with Hank’s buffer, then resuspended in 20 ml of Hank’s buffer and underlaid with 10 ml of FBS (non-heat-inactivated) containing 3 mm EDTA. After 20 min of centrifugation, the supernatant was decanted and the platelet-free HPBL pellet resuspended in 50 ml of OPTI-MEM (Life Technologies, Inc.) containing 5% autologous serum (reserved from the whole-blood sample) and 1% l-glutamine/penicillin/streptomycin solution (Sigma Co.) The cells were adjusted to 5 × 105/ml and transferred to T-150 flasks. The cell suspensions were incubated at 37°C in 5% CO2, 95% air and 100% humidity for 2 h and transferred to new T-150 flasks and incubated under the same conditions for an additional 4 h. If red cell contamination was evident in the HPBL pellet, 9 ml of sterile distilled H2O were added and resuspended, passing through a sterile transfer for 20 s. One ml of 10× Hank’s buffer was added and the final volume adjusted to 20 ml with Hank’s buffer. The cell suspension was centrifuged for 10 min at 800 rpm, and the final red cell-free pellet was resuspended in 50 ml of OPTI-MEM with 4% FBS (heat-inactivated) and 1% l-glutamine/penicillin/streptomycin solution. The final lymphocyte population was more than 95% pure as judged by light microscopy.
Stock solutions of GSH, MPG, and NAC (100 μm) were freshly prepared in RPMI before the start of the experiments, and 7.5 × 104 cells were grown in the presence of increasing thiol concentrations in a volume of 1 ml/well. Growth and viability were measured as described above.
Apoptosis and Necrosis.
Apoptosis and necrosis were determined after 24 and 48 h of incubation by morphological assessment with acridine orange-ethidium bromide staining and fluorescence microscopy (11). Cells were centrifuged in a volume of 100 μl for 15 s at 12,000 rpm. The supernatant was aspirated and the pellet resuspended in 20 μl of PBS and 2 μl of a mixture containing 100 μg/ml each of acridine orange and ethidium bromide. Aliquots of 10 μl of stained cells were observed with fluorescence microscopy, and the number of apoptotic and necrotic cells were determined by counting 100 cells in each of five different, randomly selected fields.
SOD and Catalase Activity Assays.
After a 24-h incubation in the presence of various MPG concentrations, 107 cells were washed once, resuspended in chilled 10 mm phosphate buffer (pH 7.0) containing 0.15 m NaCl, and sonicated for 30 s on ice. Aliquots were used for measuring enzyme activities. SOD activity was measured using the cytochrome c reduction assay as described by McCord and Fridovich (12). Catalase activity was evaluated according to Bergmeyer (13), using a freshly prepared stock solution of hydrogen peroxide. The protein content of crude extracts was measured using the method described by Lowry et al. (14)
SOD Activity Gels.
Aliquots of the cell lysates of 3 μl or less containing 0.1 unit of total SOD activity were loaded on agarose gels (Universal Gel/8; Helena Laboratories, Beaumont, TX) and electrophoresed in 20 mm Tris-glycine buffer (pH 8.2) at 200 mA for 15 min. The gel was stained for SOD activity with nitroblue tetrazolium-riboflavin-EDTA and then developed under fluorescent light as described by McCord (15).
Genomic DNA was extracted from Jurkat cells by phenol/chloroform and ethanol precipitation. SSCP analysis was performed on genomic DNA samples as described in the FMC BioProducts (Rockland, ME) protocol by using primers for a portion of exon 3 of the sod2 gene and the Mutation Detection Enhanced gel system. The primer sequences were: WV5 5′ (5′CAGTGGTTGAAAAAGTAGGAG3′) and WV31 3′ (5′TTAGGGCTGAGGTTTGTCCA3′). This amplification yielded a 148-bp PCR product.
RT-PCR and Sequencing of Jurkat sod2 cDNA.
Total RNA was purified from cell extracts using the total RNA purification system (Promega, Madison, WI). Samples of total RNA were reverse transcribed and amplified by PCR using the SF3 5′ (5′-CCCCTGCAGATGTTGAGCCGGGCAGTGTGCGGCACCAGC-3′, and the SF4 3′ (5′-CCCTCTAGATTACTTTTTGCAAGCCATGTATCTTTCAGT-3′) primers with the Access RT-PCR system (Promega) as described by the manufacturer. The PCR products were purified and cloned into the TA cloning kit from Invitrogen Life Technologies (Carlsbad, CA). Colonies were screened by PCR, followed by restriction analysis with HaeII and MboII. Clones with inserts of the right size, orientation, and restriction sites were sequenced with Ampli Taq DNA polymerase FS with the dRhodamine terminator cycle sequencing-ready reaction kit at the DNA sequencing and analysis core facility of the University of Colorado Cancer Center. Results were analyzed with PCGENE software.
Statistical significance was determined by one-way ANOVA using the Neuman-Keuls test by StatMost software for PC.
Jurkat cells were grown in the presence of several different reducing agents such as MPG, NAC, and GSH with the intent of modifying the oxidative state the cells. The effects of MPG on growth are shown in Fig. 1,a. After 24 h, low MPG concentrations ranging from 0.03 mm to 1.0 mm inhibited proliferation. Higher MPG concentrations of 3.0 mm and 10.0 mm, however, produced no inhibitory effects. We also quantified the percentage of apoptotic and necrotic cells under each condition, as well as cell viability (Fig. 1,b). Surprisingly, we found that only the intermediate concentration of MPG (0.1 mm) induced the highest percentages of apoptosis (40%). In cultures incubated with 0.3 mm and 1.0 mm MPG, the cells died mainly by necrosis. Cell viability assessed by trypan blue exclusion was minimal at concentrations between 0.1 and 1.0 mm, essentially mirroring the necrosis. The highest concentrations of 3.0 mm and 10.0 mm MPG produced no observable adverse effects; by all criteria, these cells resembled control cells (Fig. 1 b). Thus, the growth rate of Jurkat cells is affected by increasing concentrations of MPG in a complex and multiphasic manner.
NAC and GSH, used at the same concentrations as MPG, produced effects similar to those seen with MPG. Fig. 1,c shows the proliferation of Jurkat cells incubated with increasing concentrations of NAC. Cells incubated with 0.03 mm NAC showed no effects, but concentrations ranging from 0.1 mm to 10 mm inhibited the proliferation at 24 h. Cells incubated with concentrations from 0.3 mm to 3 mm of NAC showed the highest percentages of apoptosis (Fig. 1d). The percentage of necrotic cells was high only in the cells incubated with 1.0 mm NAC, again reflected as low viability assessed by trypan blue exclusion.
When GSH concentration was very low (0.03 mm or 0.1 mm) or very high (3.0 mm or 10.0 mm), there were no differences in growth compared with control (Fig. 1,e). Cells incubated with intermediate concentrations of 0.3 mm and 1.0 mm, however, showed diminished viability and growth. The percentage of apoptotic cells was highest at a GSH concentration of 1.0 mm (Fig. 1,f). The percentage of necrosis was highest in cells incubated with 0.3 mm and 1.0 mm GSH, correlating inversely with the percentage of viable cells (Fig. 1 f).
We then evaluated the effects of MPG on the specific activity of SOD, in intact cells and in cell-free extracts. Fig. 2,a shows that SOD specific activity decreased about 75% compared with control, as MPG in the culture medium was increased to 0.1, 1, and 3 mm. Also, extracts from control cultures grown in the absence of MPG were treated in vitro with various concentrations of MPG. A dose-dependent loss of SOD2 activity was observed (Fig. 2 b).
These SOD specific activity assays measured the sum of the two intracellular forms of SOD, the cytosolic SOD1, and the mitochondrial SOD2. Therefore, we determined the relative contributions of SOD1 and SOD2 by native agarose gel electrophoresis and an activity stain. Fig. 3 a shows that the major band of SOD1 is not different from the control (HPBLs), although an additional weaker, faster moving band was visible in the Jurkat extract. The SOD2 of Jurkat cells, however, differs from that of normal lymphocytes quantitatively as well as qualitatively. The SOD2 activity is lower in Jurkat cells, as described previously for transformed and cancerous cells. In addition, the electrophoretic mobility is slightly faster than the SOD2 of normal HPBLs, suggesting the possibility of a mutated or differently processed SOD2 in Jurkat cells.
To pursue the possibility of a mutation in the Jurkat sod2 gene, we performed SSCP of exon 3 (in which the previously identified point mutation I58T is localized) to assess the possibility of a similar mutation in the Jurkat sod2 gene. We found an anomalous SSCP pattern in this region compared with the wild type (Fig. 3 b), strongly suggesting a mutation in the gene.
The Jurkat sod2 cDNA was cloned and sequenced. Five of six independent clones (designated Jurkat Allele 1, Fig. 4) contained the C1183T substitution (which encodes the A-9V variant) in exon 2, previously reported by Heckl (16). These five clones contained wild-type sequence in the entire region coding the mature SOD2. In one of six independent clones, however, we found a cDNA sequence presumably reflecting the other Jurkat allele (designated Jurkat Allele 2, Fig. 4). This sequence was wild type at the C1183 locus, but contained a new point mutation, C5782T, in exon 3, that will produce a L60F mutation in the enzyme. Thus, Jurkat T lymphocytes appear to be heterozygous in the sod2 gene at two loci: the previously described −9 locus and at a previously undescribed locus, 60.
When the SODs were first discovered, it was assumed that their roles were primarily to protect and defend against a cytotoxic metabolite. More recently, however, it has become apparent that the radical serves rather subtle metabolic regulatory roles, even in perfectly healthy cells. Superoxide has been proposed as a possible second messenger in the transduction of metabolic signals (17). Low concentrations of superoxide regulate the proliferation of resting human lymphocytes (1) probably because of the activation of proteins related to growth control, such as kinases and some transcription factors (AP-1, NF-κβ, c-jun, c-fos; Refs. 18, 19). Some of these proteins have been shown to be redox controlled, and their modification by oxidants may activate the cells to proliferate. However, the original premise holds true as well; high concentrations of superoxide can cause cell death (1) by inducing lipid peroxidation, protein inactivation, and DNA damage at the molecular level, as well as by inducing the phenomena of apoptosis and necrosis.
Jurkat T-cells proliferate in culture without activation, a characteristic common among immortalized cells. The effects of several thiol-reducing agents on growth and proliferation were evaluated, with results that appear to be complex, multiphasic, and paradoxical. The growth of Jurkat cells progressively slows as thiol concentrations increase toward 1 mm (Figs. 1, a, c, and e) but is dramatically restored to normal at 3 mm or higher. Fig. 1 b sheds more light on what is happening in the growth-inhibitory range of thiol concentrations by revealing that the cells are dying almost entirely by apoptosis at 0.1 mm MPG, but that the mode of death shifts to predominantly necrosis at 1 mm MPG. This progressive behavior of “proliferation to apoptosis to necrosis” is reminiscent of how primary human peripheral lymphocytes behave in the face of increasing rates of superoxide production (1). The apparent paradox is that our previous study observed this progressive behavior as cells were exposed to increasing oxidative stress brought about by the redox-cycling agent paraquat, whereas the present study sees the same behavior as cells are exposed to increasing concentrations of a reducing agent, MPG.
We begin to understand the paradox with the data seen in Fig. 2,a. Very low concentrations of MPG in the growth medium markedly decrease the SOD activity in Jurkat cells, and similar concentrations inhibit the enzyme in cell-free extracts (Fig. 2 b). This inhibition of SOD2 by thiols was unexpected because Matsuda et al. (20) have reported that the activity of wild-type SOD2 is unaffected by thiols. We have found, however, that a mutant form of the enzyme, I58T, is quite sensitive to inhibition by thiol reagents (21), probably through the formation of mixed disulfides. This mutant form of SOD2 has been characterized by Borgstahl et al. (22) and found to exist largely as a dimeric structure rather than the normal tetramer. In the dimer, each subunit exposes cysteine-140, a residue normally buried in the tetrameric structure. Thus, we wondered whether Jurkat cells may contain the I58T mutant form of SOD2 (or a similar mutation), which might explain the abnormal sensitivity to thiol reagents. If this were true, low concentrations of thiols could paradoxically create a condition of oxidative stress by virtue of SOD2 inhibition, resulting in increased numbers of apoptotic and necrotic cells. At high concentrations of the thiol reagents (3–10 mm), the antioxidant protection provided by the thiols might make up for the loss of SOD2 activity or, at the very least, drive the protein mixed-disulfides back to their reduced states to reactivate the SOD2, decreasing oxidative stress and allowing restoration of normal growth. The formation of protein-mixed-disulfides (or S-thiolation) under conditions of mild to moderate oxidative stress is well documented, and a number of metabolic enzymes are known to be inactivated by such modification (23). If a mutant SOD2 were susceptible to inactivation by S-thiolation, the resulting elevation in oxidative stress would only serve to promote additional S-thiolation reactions, leading to a possible vicious cycle within the cell.
An examination of the electrophoretic behavior of Jurkat SOD2 (Fig. 3,a) was consistent with our hypothesis that the SOD2 might be a mutant form. The electrophoretic pattern differed from that seen in normal HPBLs, both quantitatively and qualitatively. There was less SOD2 activity, and its mobility was clearly faster than that of the wild type. This difference in mobility could reflect a coding mutation, or it could be evidence of S-thiolation of the enzyme, or both. Confirmation of a mutation was provided by SSCP analysis (Fig. 3 b). The pattern presented by an amplicon containing exon 3 (where the I58T mutant is located) is clearly different from wild type. The pattern is consistent with a heterozygous genotype because the wild-type pattern is present, together with a similar but faster moving pattern, presumably contributed by the mutant allele.
Cloning and sequencing of the Jurkat sod2 cDNA (Fig. 4) clearly revealed that the cells are indeed heterozygous. One allele contained the common alanine-to-valine mutation at residue −9 (in the leader sequence) in exon 2, but was wild type in exon 3. The other allele was wild type at −9, but contained a new mutation, leucine-to-phenylalanine, at position 60 in exon 3. This L60F mutation is only two residues downstream from the I58T mutation previously studied by Borgstahl et al. (22) The location of the I58T mutation is in a two-helix bundle that extends finger-like from each subunit (Ref. 24; the protein data bank coordinates were visualized with the software Rasmol). The monomer-monomer interface of human SOD2 is typical of globular protein subunit interactions, involving a substantial area of contact. The dimer-dimer interface, however, is quite atypical, involving a small area of contact between two pairs of these finger-like two helix bundles. These areas of contact involve only about eight residues contributed by each subunit. The eight contact residues include both the isoleucine residue at position 58 and the leucine residue at position 60. Thus, it seems highly likely that the L60F mutation that we report here from Jurkat cells will be functionally similar to the previously studied, tetramer-destabilizing I58T mutation, (22), which renders the enzyme thermally unstable and vulnerable to inactivation by S-thiolation reactions (21).
The finding of a destabilizing SOD2 mutation in a leukemia-derived cell line may not be coincidental. Nearly all cancerous tissues, including leukemia lymphocytes (4), have decreased SOD2 (and increased oxidative stress) leading some to regard sod2 as a tumor suppressor gene (25). Exposure of mitotically competent cells such as fibroblasts (26) or lymphocytes (1) to increased production of superoxide induces the cells to proliferate. Thus, a nearly universal feature of malignantly transformed cells may be either an increase in production of superoxide or a decrease in endogenous SOD levels (27), with the latter seemingly more common. The decrease in SOD activity could occur via misregulation of the gene (resulting, e.g., from mutations in the promoter region of the gene) or via coding mutations such as L60F that may result in a thermally unstable enzyme or in an enzyme that is susceptible to inhibition by the formation of mixed-disulfides involving sensitive thiol groups exposed by the weakened tetrameric interface. It is interesting to note that SOD1 of chickens and of humans exists as S-thiolated derivatives, reflecting mixed disulfides with GSH (28). It is also noteworthy that the bizarre behavior toward thiol reagents that we describe here for Jurkat cells is not unique, but has been reported by Morse et al. (9) for CEM cells, another human leukemia-derived cell line. Thiols represent an important class of antioxidants that contribute to cellular homeostasis in many ways. We report here an unusual mutation that renders SOD2, a vital antioxidant enzyme, sensitive to inactivation by these normally protective compounds.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Supported in part by a grant from the Gustavus and Louise Pfeiffer Foundation, and the Centro de Investigaciones Biologicas del Noroeste, La Paz, BCS, Mexico.
The abbreviations used are: SOD, superoxide dismutase; MPG, N-2-mercaptopropionyl glycine; GSH, glutathione; NAC, N-acetylcysteine; SSCP, single-stranded conformational polymorphism; HPBL, human peripheral blood lymphocyte; FBS, fetal bovine serum.
We thank Drs. Sonia Flores, Carl White, and Bifeng Gao for their technical assistance and helpful discussions.

domain: literature


Port Arthur police calling for several changes in the city's game room ordinance on Tuesday. This comes after investigators busted as many as 7 illegal game rooms within the last six months.
Now, they are asking city officials for help to put a stop to the illegal gaming business.
"Gaming sites that we have shut down in the last three months, their actually some that never attempted to get their proper paper work they just opened up." said Officer Michael Hebert.
Through a presentation, PAPD explained some of the requirements listed in the new ordinance such as:
- Permit process documentation
- Car parking requirements
- Restricted operating hours
- Increased application fee
- Reduced regulatory license fee
- Fingerprint background checks for owners and operators
- Business and building ownership verification
"This is a very important issue that's affecting the entire community and it crosses several departments so we want to make sure we get it right." said Chief Patrick Melvin.
Investigators say there are approximately 60 gaming locations in Port Arthur and have issued 175 citations within the last three months.


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As unemployment continues to cripple the country's economy, one sector surely experiences growth every winter: personal trainers. Well, it certainly seems that way every February, when dozens of major leaguers report for spring training and pronounce themselves in "the best shape of their life," thanks to diet and, of course, "hard work."
A few years ago, large gains or losses in weight would send the performance-enhancing drug police into overdrive, speculating that Player A's chiseled pectoral muscles surely were not natural, or that Player B's huge weight loss surely had less to do with cutting out fast food and more to do with cutting out Deca-Durabolin.
While it's certainly plausible - likely? - that nefarious acts still occur in weight rooms over the winter, stronger drug testing has made the winter wake-up calls a bit less tense. Still, every spring training brings tales of specialized diets and weeks spent toiling at elite performance centers. Here's our first batch of such anecdotes, featuring, among others, a bulked-up Mets star, two slimmed-down Cubs and a leaner, but apparently no less powerful former NL MVP:
David Wright "worked out with more passion and changed his diet."
The Yankees' Nick Swisher lost 12 pounds.
Ryan Howard attended the Athletes Compound in Tampa, lost "a lot of weight" and is down to a solid 245 pounds.
Carlos Zambrano says he lost "12 to 15 pounds" thanks to a new diet.
But Zambrano has nothing on his Chicago Cubs batterymate. Geovany Soto lost 40 pounds after he hired a personal trainer and stuck to a strict diet this winter. "I wasn't strong," Soto told the Chicago Tribune when asked if there was any possible link between his weight loss and performance-enhancing drug use. "I was just fat."
Rockies starter Aaron Cook "wanted to lose a little weight" after a shoulder strain curtailed his 2009 season. He lost 18 pounds.
The Phillies' Kyle Kendrick worked out hard in a bid for his team's fifth starter job.
Ken Griffey Jr. lost just seven pounds. But he's 40, so that's kind of a big deal.
Matt Hobgood, who probably won't see the Baltimore Orioles' rotation until at least 2012, nonetheless says he lost 17 pounds of fat and added nine pounds of muscle, reducing his body fat from 22% to 17%.
Oh, and he won't throw a pitch or swing a bat, but Phillies manager Charlie Manuel had the biggest loss of all - 58 pounds, while picking up a weight-loss endorsement.
By Gabe Lacques
Daily Pitch is a baseball fan's destination for breaking news, emerging trends and enlightening banter. With a veteran group of reporters positioned in three time zones, we provide a balanced, comprehensive glimpse at our national pastime, with a nod to both the passion and complexities that make the sport irresistible to many. More about our reporters


Here are deals for the VGOD Pro Mech Mod or Pro Mech Kit. Made from durable billet copper with a beautiful matte coating and gold engraving, this mech mod was explicitly made for veteran vapers. Unfortunately, this mech mod is no longer available. Try the mech mod deals below instead.
Designed by vape veterans, the VGOD Pro Mech Mod or Lot features an exquisite design that will turn heads. This setup is used almost solely by trick benders and flavor cravers, so beginners should stay away. It is also considered a high-end device because of the billet copper material used in building it. A carrying case is also included. The main features of this mech mod are the large 24mm diameter, the firing button that features aerospace-grade carbon fiber, and the self-adjusting contact that ensures the best possible conductivity.
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#AceNewsReport – Sept.04: A 17-year-old boy and two men, aged 21 and 22, were detained and taken to hospital, where they remain: Their injuries are not believed to be life threatening: A 30-year-old man was arrested on suspicion of possession of an offensive weapon. He remains in police custody for questioning:
GM Police Officers are investigating incident in Stockport of a number of stabbings on Tues.01: Sept:2020: and are calling for witnesses
Chief Inspector Helen Smith, of GMP’s Stockport district, said: “This incident has left three people in hospital – thankfully, their injuries are not believed to be life threatening and they are all expected to make full recoveries: One person is in police custody: Meanwhile, enquiries are ongoing to establish the full circumstances surrounding the incident: “We know and understand that this incident has caused concern but I would like to use this opportunity to reassure members of the public that we believe: that this was an isolated incident; that we have identified all of those involved and that there is no risk to the community: I would also like to stress that this is not in any way linked to the nearby school: “Whilst enquiries are ongoing, officers are developing a clear understanding of what happened last night but, as always, I would appeal to anyone with information which may assist us to contact police: You can do so via phone, online or via the extra officers who are patrolling the area: If you would prefer to share information anonymously, please contact Crimestoppers.”
Anyone with information should contact police on 0161 856 9629 quoting 1969 of 01/09/20 or the independent charity – Crimestoppers, anonymously, on 0800 555 111.
#AceNewsDesk report …………….Published: Sept.04: 2020:
Editor says #AceNewsDesk reports by https://t.me/acenewsdaily and all our posts, also links can be found at here for Twitter and Live Feeds https://acenewsroom.wordpress.com/ and thanks for following as always appreciate every like, reblog or retweet and free help and guidance tips on your PC software or need help & guidance from our experts AcePCHelp.WordPress.Com

domain: law


The GL06 pistol light features a 600 lumen high-intensity beam to identify targets from afar, strobe to disorient adversaries, versatile rail compatibility, and a quick-release design. Furthermore, this patented replaceable rail key design ensures secure attachment to Glock and Picatinny rails. Accompanied by a patent-pending lever latch system, the GL06's ambidextrous switching can be activated with one finger - momentary or constant-on. This light is powered by an ARB-L16-700UP battery that can be charged via USB cable or compatible battery chargers. The GL06-365 variant is designed to fit SIG Sauer P365 / P365XL and other handguns with similar rail dimensions.


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RSU is reported on your W-2. It's figured by taking gross number of shares vesting * per share FMV at vesting date.
Your per share fair market value (FMV) for the stock is the same per share figure your employer used to calculate the compensation and that's the per share figure you'll use when the sale is reported to you.
If you are worrying about somehow not being able to enter the taxes paid with the cash raised from the stock sold "for taxes", then I know you are making the mistake of using the wrong basis to report the sale and seeing your tax liability go up when you do. The answer isn't to somehow report the withheld taxes again - they are already included on your W-2 and you can't deduct them again, the answer is to use the correct basis when reporting the sale.
As TurboTaxCherylW, EA, MsTax pointed out your correct per share basis is the same as the per share "fair market value" used by your employer to determine the compensation created by the vesting.
Enter the 1099-B exactly as it reads and then click the blue "I'll enter additional info on my own" button. On the next page enter the correct basis in the "Corrected cost basis" box. The correct basis is: (# of shares sold) x (per share basis for that lot.)
(SINCE THE DEVELOPERS CHANGE THE SECURITY SALE INTERVIEW EVERY SINGLE YEAR I'LL NOTE THAT THIS ANSWER'S DIRECTIONS ON HOW TO CORRECT THE BASIS FOR THE SALE PERTAINS TO THE 2016 INCOME TAX YEAR. I'M SURE THAT THE INTERVIEW WILL CHANGE IN THE YEARS AFTER THAT.)

domain: finance


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Spacious 8-Bed Shared Room in beautiful Portuguese farmhouse, great value for money. 4 bunk beds, for 8 adults. Wide range of accommodation with a welcoming and friendly atmosphere. Hidden in the countryside but just five minutes from Lagos.
There's a laid back atmosphere and plenty of places to sunbathe, read or just hang out with friends. We have a huge garden, sun loungers, hammocks, bean bags, and a yoga area to make your stay as relaxing as possible. Outdoor staffed bar with cold drinks and snacks. Surf Lessons or other activities, like mountain biking, climbing, wakeboarding, stand up paddleboarding etc. can be booked on site at our activities desk.
Free Wifi. Breakfast, fresh linen and towel included.
Servicios y zonas comunes
Free parking space available for guests.
Atención a los huéspedes
Our dedicated staff is on-hand to answer all your questions and assist with booking activities, throughout your stay.
Reception Desk onsite for smooth check in & check out.


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- iRhythm Technologies presented an analysis of Medicare claims data showing its Zio XT outperforms other ambulatory cardiac monitors, boosting the company’s bid to win market share.
- The U.S. market is less than 30% penetrated, according to analysts at William Blair. Supported by evidence such as the Medicare data, the analysts expect Zio XT and other long-term cardiac monitors (LTCM) to ultimately capture most of the ambulatory cardiac monitor market.
- iRhythm is looking to its next-generation Zio monitor to drive the transition. In a second study presented at the American College of Cardiology (ACC) Conference, the company showed its new device achieved a higher arrhythmia yield than its predecessor.
Physicians have multiple options for monitoring cardiac rhythm. iRhythm claims its single use wearable Zio monitors are more accurate and lower the cost of care, but many physicians continue to prescribe Holter, mobile cardiac telemetry and ambulatory event monitor devices, as well as rival LTCMs.
To compare the effectiveness of the different options, researchers analyzed data on almost 288,000 Medicare fee-for-service patients who received an ambulatory cardiac monitors for the first time in 2017 and 2018. Most, 54%, of the patients received Holter devices. LCTMs accounted for 13% of the dataset. Users of Zio XT had a higher diagnostic yield than and lower 180-day retest rate than other devices, including rival LTCMs.
“The data supports our belief that LTCM will make up the majority of the ACM market over time,” analysts at William Blair wrote in a note to investors. “With the market less than 30% penetrated domestically, we believe this data should help drive further awareness and adoption not just in the United States, but globally over time.”
Other LTCMs were 20% less likely to detect a specified arrhythmia and 3.51 times more likely to require a retest within 180 days. Zio lists Philips’ BioTelemetry, Boston Scientific’s Preventice Solutions and Baxter’s Bardy Diagnostics as the competitors to its Zio services.
The authors of the analysis included iRhythm consultants and employees.
iRhythm also used the ACC event to promote data on its next-generation device. The device, which is smaller and lighter than its predecessor, received clearance from the U.S. Food and Drug Administration in 2021 but is still in a limited launch. Ahead of the full launch later this year, iRhythm linked the device to a higher arrhythmia yield than Zio XT. The device beat its predecessor by 80.9% to 77.1%.


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Paul Revere's Ride Paperback – March 1, 1996
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Ted Rand--well-loved illustrator of The Hullabaloo ABC, Mailing May, Knots on a Counting Rope, and many other critically acclaimed titles--masters the mood and movement of the famous midnight ride, and children will love the power and drama of this historic American event. (Great read-aloud, ages 4 to 8) --This text refers to an out of print or unavailable edition of this title.
From Publishers Weekly
Copyright 1990 Reed Business Information, Inc. --This text refers to an out of print or unavailable edition of this title.
Top Customer Reviews
The courageous patriotism exemplified by Revere makes him an admirable hero, especially for young boys. Ted Rand's illustrated version of Longfellow's work visually represents the suspense and danger of the night, allowing young readers to experience the story before they have fully grown into the poem. The dramatic scenes work well with the rhythmic action of the poem, and while turning the pages one can almost hear the horse's hooves pounding the road as Revere rides on and on.
The book contains a brief historical afterword which helps contextualize Revere's ride in the events of the Revolution, and inquisitive readers can also explore the online Paul Revere Heritage Project or the website of the Paul Revere House.
"Listen, my children, and you shall hear
Of the midnight ride of Paul Revere,..."
and this book is a wonderful introduction to the full poem for the next generation. There are vibrant illustrations which are not only attractive but really draw you into the mood of the story. The beginning and end of the book have a map charting out his ride, so that you can follow along visually. Finally, at the end, there is background information about the events that prompted this ride and the end result of it.
I read this book with my 7 yo and 5 yo. First, I gave a very brief 15-second background explanation, and it helped that they had a very basic knowledge of the Revolutionary War and are used to me reading to them more advanced books. Then I read the poem, with them looking at the pictures, only rarely stopping to explain a particular word, and then went through the explanation at the end. They enjoyed it, and were drawn into the poem. Even though the words are complex, if read well, and with the accompaniment of the pictures and basic background understanding, it can be engaging to the younger set. I expect they will get more from it as they get older, and I plan to be reading this book with my children at least once a year as part of our homeschooling. Definitely would recommend this book for the elementary-aged set, and I will probably give it as a gift to my nieces and nephews in the future.
Most Recent Customer Reviews
Although the language was tricky for my younger kids (4-6 yes old), we loved this book when their dad brought it back from a trip to Boston, and loved it even more when we took it... Read morePublished 29 days ago by BMmussman
This was a great book for my 4th graders. We were doing a unit on narrative poetry, and this was a great mentor text. The kids loved it!Published 4 months ago by NogV
I enjoyed reading this story of an historical event during the U.S.revolutionary war. There were no cell phones then for communicating. The artwork in this book is extraordinary.Published 9 months ago by Catherine Hagerup
Beautifully illustrated. Gave me chills as I read it to my children, and the loved it.Published 11 months ago by MJB
I purchased 2 copies of this book for my friends children. I was impressed with the illustrations in the book, and that it's the original story from Henry Wadsworth Longfellow. Read morePublished 12 months ago by Stephanie
This Kindle edition DOES NOT contain illustrations as advertised. It is nowhere near the 46 pages indicated. Read morePublished 13 months ago by BertandTam

domain: library


Researchers at NYU-Poly created a unique game-based computer program to educate, train, and disseminate critical information to both career and volunteer firefighters. Advanced Learning Through Integrated Visual Environments (ALIVE) features short interactive training modules that incorporate game techniques and simulate the real-time critical decision-making process. Applications for mobile devices are also available for download.
Since its inception, ALIVE has been adopted as a training tool by more than 100 fire departments that encompass more than 25,000 firefighters, including those from New York City and Chicago—two of the largest departments in the nation.
ALIVE was funded by the U.S. Department of Homeland Security and led by NYU-Poly in partnership with the Fire Department of New York, the Chicago Fire Department, and the fire departments of Bloomington, Eden Prairie, and Eagan, Minnesota. Tests of 600 firefighters determined that individuals who trained using ALIVE scored consistently higher than those who received classroom training.
“We were stunned that the firefighters did so much better with ALIVE than in the classroom—it is rare that results are this strong and consistent,” says NYU-Poly professor of environmental psychology Richard Wener, one of the leaders of the research project. ALIVE research leader Sunil Kumar is professor of mechanical engineering at NYU-Poly and dean of engineering at NYU Abu Dhabi; Prabodh Panindre, a senior research scientist at NYU-Poly, is also a member of the project.
Working through the interactive ALIVE segments, firefighters learn more than just how to fight a fire: They learn the science behind the fires. Achieving an understanding of fire dynamics is of paramount importance to today’s firefighters since the characteristics of modern residential construction—including large, open layouts, increased fuel loads from synthetic materials and furnishings, and minimal fire resistance of structural components—make modern residential fires far different from those of past generations.
Two modules of ALIVE, “Wind-Driven High-Rise Fires” and “Fires in Residential Lightweight Construction,” have been released to the nationwide firefighter community free of charge, along with iOS and Android versions.


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To see a breakdown of your invoice and determine how much was charged for both your subscription and SMS usage go to Setup > Subscription > Invocies and Receipts. Under the Summary column your total invoice amount will be broken down into subscription and SMS fees.


The Tu-126, with the complex "liana", could satisfy the requirements of the fight with aerial targets in the second-half of the 1960's, with the the aircraft of the NATO alliance countries strike aviation to operations at the small and supersmall heights. One of the essential deficiencies in the complex "liana" was its incapacity to isolate the low altitude targets against the background of the earth. The special trainings of the crews of the Tu-126 made it possible to reduce the flight altitudes of the aircraft, from which the complex could "illuminate" by its radar from below. However, this was only a partial solution. No training of crews could raise Tu-126 to the level of the requirements on the fight with the aerial targets, advanced in the 1970's.
The Soviet air defense system required a new complex, capable of tracking low altitude targets against the background of the earth. Thus, already up to the moment of developing the Tu-126 complex, in light of the new requirements, it began gradually to grow antiquated. Taking into account the situation in the second-half of the 1960's, the USSR began scientific design work on the creation of a new aviation complex of the long-range radio-location [radilokatsionnogo] patrol, capable of effectively working on low altitude purposes.
The design was started on a new "bumblebee" radio-location complex with a radar of distant observation, with the antenna system "mushroom", analogous to that adapted on the Tu-126. The complex had to ensure detection and data processing about the high-speed unobtrusive purposes, which fly at a distance to 200 km in the lower hemisphere and to 500-600 km in the upper. In 1969 work started on the government solution beginning of the development of a new carrier aircraft under the "bumblebee" complex. The demand of servicemen on the use of one of the series-production heavy machines as base aircraft became one of the conditions, which determined the direction of the forthcoming work. [MMZ] "experience", as from the middle of the 60's began to be called the experimental plant of #156, and OKB A.N. Tupolev on the basis the government solution was connected to the works on the creation of a promising new DRLO aircraft.
In 1970 is prepared preliminary design on this theme, which received the OKB designation aircraft "156". In the preliminary design for the DRLO aircraft examined the use of several machines under development at OKB-156. Designers studied the possibility of using as the base machines for the complex "bumblebee" of series Tu-142[M], Tu-154 and Tu-126. The first they rejected because of the complexity of the normal arrangement of equipment of the "bumblebee" complex on the aircraft because of the small diameter and the volume of fuselage. But the VVS insisted precisely on Tu-142[M], which repeated the history with the initial selection Tu-95 for Tu-126. The Tu-154 required essential alterations, furthermore, it had comparatively small duration of flight. Aanalysis showed, that of Tupolev's machines for this purpose, in spite of a number of negative special features, the Tu-126 was most appropriate. However, in 1968 the production of the aircraft was ended, and entire production line was dismantled. Therefore all reasonings around it bore purely "academic" nature.
The analysis of carrier aircraft of complex "bumblebee" in this case gave source material for designing the new aircraft. In the consequence under the complex "bumblebee", Tupolev prepared the preliminary design of the new carrier "156" with four D-30[Kp] engines , close in overall design to American E-3A. As the base in this project adapted entirely the Tu-126. Differences on the fuselage and the equipment were reduced to the following. Because of the need of guaranteeing the normal aperture in the lower hemisphere the fairing about "mushroom" was established doubly on the higher pylon. An increase in the volume of the installed equipment required an increase in altitude of forward fuselage section in the region of the first section - on one of the alternative designs came out specific hump according to the type of the Boeing 747. For the survey of forward half sphere in the nose section of the aircraft the "barrier" RLS was placed with the phased antenna array, and in the system of defensive armament were included the controlled air-to-air missiles, developed for the interceptor E-155[Mp] (MiG-31).
Under the effect of "Vietnam syndrome" it was decided to return to the stern remote gun turret (30-millimeter gun [TKB]-635 and fire-control radar of the type "helium". Navigation and pilotage equipment renewed, the new, more effective communication system with the control centers, the fighters PVO (Air Defense) and the ships was established, were introduced the new systems of passive [RTR], new systems RAP and so forth.
However, so radical a proposal was not accepted - the customer insisted on the use of a production aircraft, in particular, Il-76. In this situation maximum, what fell to the lot Tu-126, to become a testbed for finalizing of the elements of complex "bumblebee". One of the machines was transmitted in OKB G.M. Berieva in Taganrog, where under the management A.K. Konstantinov on the base of Il'yushin machine occurred the creation of the aircraft [DRLO] of the new generation - A-50.
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Picturesque Luxembourg, with approximately 130 castles, has long been a tourist attraction. Among the points of greatest attraction are Vianden; Clervaux, with its castle of the De Lannoi family, forebears of Franklin Delano Roosevelt; the famous abbey of Clervaux; Echternach, an ancient religious center; the Moselle region; and the fortifications of the capital. Popular sports for both residents and visitors include swimming, hiking, rock climbing, cycling, and golf. More than 5,000 American soldiers are buried at the American Military Cemetery near the capital, including Gen. George S. Patton.
Most visitors from Western Europe and the US require a passport, but no visas are necessary for stays of less than three months. There are no restrictions on foreign currency exchanges by visitors.
In 2000, there were about 807,000 visitor arrivals. The country had about 7,708 hotel rooms. The average cost of staying in Luxembourg, according to 2002 US government estimates, was $186 per day.

domain: geography


Four Fronts named to Team Ontario
Rookie forward Spencer Watson is one of four Kingston Frontenacs named to the Ontario team that will play at the World Hockey Under-17 Challenge in Quebec, Dec. 29 to Jan. 4.
Kingston Frontenacs’ Doug Gilmour was one proud and pleased general manager Thursday.
The source of the pride was Team Ontario’s announcement of its 22-player roster for the World Hockey Under-17 Challenge in Victoriaville and Drummondville over the Christmas holidays.
In what is unprecedented, the Frontenacs have four players selected — forwards Sam Bennett and Spencer Watson and defencemen Roland McKeown and Dylan DiPerna.
“I am very proud of all the kids. To have four 16-year-olds all go, that is special,” Gilmour said.
Troy Smith, head coach of Team Ontario, contacted Gilmour Tuesday. In turn, Gilmour called the players Wednesday following practice.
“You could hear the excitement in all of their voices. It is very special. I am excited for all of them,” Gilmour said.
Watson is tied for Kingston’s scoring lead with eight goals and 17 points. He is fifth in rookie scoring.
Bennett has six goals and 12 points and three game-winning goals. McKeown has 12 points and is the top rookie defencemen for scoring. DiPerna has two points and has a plus rating on defence for Kingston.
The four Kingston players were all selected in the Ontario Hockey League 2012 Priority Draft. McKeown and Bennett went in the first round and DiPerna and Watson were the 23 and 24th players selected overall.
“I think people are seeing Bennett now and wondering how he wasn’t taken earlier,” Gilmour said.
“Watson and DiPerna were first-round players, too. Everybody knew Watson was a good player. It is nice for us to have them.”
No other OHL team placed more than two players on the Team Ontario roster. Belleville (Daniel De Sousa and Niki Petti) and Owen Sound (Jacob Middleton and Jaden Lindo) each had two players named. Five teams didn’t have a player selected.
The World Hockey Challenge will be held from Dec. 29 to Jan. 4 in Victoriaville and Drummondville. Gilmour said Kingston will be without its young stars for six games.
“We are going to miss them for six games but at the same time it is a great experience for them. They are going to come back here with even more confidence,” Gilmour said.
Connor McDavid, the Erie Otters exciting rookie star, is on the team too. McDavid is a former teammate of Bennett and McKeown. Bennett played left wing for McDavid with the Toronto Marlboros.
“It would be cool if they put Sam on the wing with McDavid again,” Gilmour said.


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Your wedding and bridal shower guests will be delighted when you serve them delicious treats in these exclusively designed, personalized
wedding themed pattern goodie bags. Made of high quality paper, each bag comes with a self sticking label that ...
Square Favor Boxes with Personalized Baby Shower Labels
Don't judge a book by its cover, but do judge a favor by its square favor box with personalized baby
shower labels! These charming little gift boxes come in your choice of design and cardstock color: white, black, or ...
Add some rustic romance to your wedding reception with these beautiful little favor boxes. Each box is made of sturdy
woodprint cardstock and features a die cut heart window that shows the word "Love" at its center. Simply fill ...
For an easy way to cheer on both wedding guests and newlyweds alike, these boxes are a sure bet. Each
pedestal shaped box comes with a large, colorful tissue paper pom pom. Simply fill it with your favorite sweets ...
We believe in celebrating life - every occasion, every milestone, every laugh, smile and tear. We know that sharing these moments makes them extra special, and we want to be there to help you celebrate life, one event at a time.

domain: design


text:
Meharry-Vanderbilt Alliance discussion addresses race and health equitySep. 23, 2016, 9:00 AM
Dr. Camara Jones, president of the American Public Health Association and a senior fellow at both the Satcher Health Leadership Institute and the Cardiovascular Research Institute, shared allegories on race and racism at a roundtable discussion at a recent Meharry-Vanderbilt Alliance event.
“It’s not a scary thing to identify racism,” Jones told community members gathered at the Meharry-Vanderbilt Alliance on Monday, Sept. 12. “It’s an empowering thing.”

domain: sociology


text:
26, the6, pink hair, tattoos,Vegan, In love "Sometimes I hate you, but always I miss you."
11784 Total post views
Nikki Lee (@nikkileeisme)
I am a digital artist who draws badass women and I like experimenting!
www.danwal.18sexy.be <-- ꜰⅰnⅾ ⅿе аnⅾ тhоuꜱаnⅾꜱ оꜰ отhеr уоung bеаuтⅰеꜱ уоu саn hеrе.
Mattia Cecchi (@akacek)
Romantisme Pornographique (@romporn)
Kind of Agent provocateur ;) Art Director. RP is a conceptual project about Art, Nude & Altporn photography, Graphic Design, Typography, my own works.
Lucian Föhr (@lucian)
The Föhr of Berger & Föhr
Current header by @ brtleonardo
A collection of visual artists on ello. Mention @elloart with suggested artists to feature! Posts curated by artist @carlosbob (aka Kim Leutwyler). This account is not run by Ello.
Todd Berger (@todd)
Berger of Berger & Föhr
Header art by @dia
Independent Creative Developer, Musician, Multimedia-ist, Cyclist, Vegetarian.
One of the original Ello developers & co-founders.
Lead: Hatun Noguera (@ellocannabis)
Ello's Cannabis Community- Art, Legal, Political, Weed updates. Curating All of Ello for Cannabis. Please add "@ellocannabis" or #ellocannabis" in your post for re-posting. See my Curation @hatun for non cannabis postings. In the Openspace alph test I'm ATcannabis.
Hello, my name is dave i do illustrations and other artistic things. Contact me for projects and stuff
Recyclism; noun; the resituating/recycling of art,changing the focus and context in order to achieve a separate artistic agenda
A photo tribute to the world's best ritual.


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Office B of R.F.& A. Lands Division of Patrick and Henry C. Va Martinsville Henry Co. June 8th 1867 Bvt. Brig Gen'l O. Brown Asst Commissioner B of R.F. & A.L. Richmond Va General, I have the honor, respectfully to call your attention to the large number of Colored children, residing in the Division who are without schools of any description, and who never have [[strikethrough]] never [[/strikethrough]] had instruction in anything. There are about One thousand children in Henry Co. and about five hundred in Patrick between the ages of six and twenty one years, all of whom would be glad of the opportunity of attending either a day school, or a sabbath school The Freedmen in this Division, have been very industrious, since their freedom, as an illustration, the fact may be stated, that there has never been a single Grant Ration, issued to them, nor a single
Please note that the language and terminology used in this collection reflects the context and culture of the time of its creation, and may include culturally sensitive information. As an historical document, its contents may be at odds with contemporary views and terminology. The information within this collection does not reflect the views of the Smithsonian Institution, but is available in its original form to facilitate research. For questions or comments regarding sensitive content, access, and use related to this collection, please contact firstname.lastname@example.org.


Boeing has launched the larger capacity 737 MAX 10 at the 2017 Paris Air Show and has been busy taking orders.
The new aircraft has improved range and fuel efficiency through improvements including a modified wing for low speed drag reduction, while the fuselage is 66 inches longer than the 737 MAX 9.
Boeing Commercial Airplanes president and chief executive officer, Kevin McAllister says: “Airlines wanted a larger, better option in the large single-aisle segment with the operating advantages of the 737 MAX family.”
“Adding the 737 MAX 10 gives our customers the most flexibility in the market, providing their fleets the range capability, fuel efficiency and unsurpassed reliability that the 737 MAX family is widely known for.”
Boeing has taken more than 240 orders and commitments from 10 customers, and has been signing deals with customers.
Boeing and BOC Aviation signed a memorandum of understanding (MoU) for 10 MAX 10s at the Paris Air Show, and GE Capital Aviation Services converted 20 current MAX orders to the larger MAX 10.
CDB Aviation Lease Finance signed an MoU for 10 737 MAX 10s as well as 42 737 MAX 8s and eight 787-9 Dreamliners in a deal worth $7.4 billion.
TUI Group, which has 70 unfilled orders for 737 MAXs, has converted 18 into MAX 10s, and the group will take delivery of its first 737 MAX in January 2018.
Tibet Financial Leasing has committed to 20 737 MAXs, including MAX 8s and MAX 10s, in a MoU valued at $2.5 billion.
Boeing Commercial Airplanes senior vice president – global sales and marketing, Ihssane Mounir says: “We are delighted to welcome Tibet Financial Leasing as a new Boeing customer and a launch customer of the newest 737 MAX family member. As leasing companies are playing an increasingly important role in the world’s aviation sector, we are proud to support Tibet Financial Leasing’s takeoff and future expansion.”
SpiceJet has signed a MoU for 40 737 MAXs, split between 20 new 737 MAX 10 orders and converting 20 737 MAX 8s from its existing order to MAX 10s.


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22 May 2013
THE Federal Opposition’s tourism spokesman has urged operators to "hunt as a pack" to secure maximum return for the local tourism industry.
Bob Baldwin was invited by the LNP candidate for Fisher Mal Brough, to take a helicopter flight to get a clear impression of what the region has to offer.
"You have an amazing vista. I mean travelling through the hinterland, over the dam, down over the coast, on the sand, the top end of Bribie Island, I’ve got to tell you it’s very very picturesque," Mr Baldwin said after touching down at Australia Zoo.
"You just need to sell it a little bit better. You need to work together as a team or, as I term it, hunt as a pack, and make sure that you people know, beyond your own community, what you actually have here as tourism assets.
"Your competition is not the beaches as against the hinterland, one enterprise against the other; your competition is other areas, other regions, and other international destinations like Fiji and Bali."
Mr Baldwin said the region provided a wide range of experiences, from the beach through to natural attractions and adventure tourism, as well as staples like shopping.
"There needs to be a symbiotic relationship with all operators that people might come here as a day destination from the Brisbane accommodation market and what we need to do is up-sell them from one experience to come back and try another experience, so you get people coming back."
Terri Irwin, of Australia Zoo, said tourism was an ideal industry for generating income without destroying anything.
"It’s a great way to show what you’ve got and still keep things intact," Ms Irwin said.


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Alright, so you all know the story of Harry James Potter, right?
You should also know the tragic love of Severus Sunshine Snape as well, correct?
Then, I'm completely sure that you know the story of Tom Marvolo Riddle and his less than acceptable childhood.
These three promising men, all ruined by an ingenius, yet loony, old wizard.
The Emerald, the Onyx, and the Sapphire.
They were shattered.
But the Diamond, will piece them back together, even at the risk of her/his life...
(The Emerald is Harry, Onyx is Snape, and Sapphire is ol' Voldie...)
The cover ain't working, so................
sorry for no cover
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