If the fuel pump relay switch is bad then the fuel pump will not be turned on. Think of the relay like a light switch. If the switch has a bad connection then the light doesnt work.
Most cars have a fuel pump relay switch located either under the hood in a fuse/relay box or on the firewall.
If the relay is bad, then the fuel pump will not work.
Yes, it is a fuel pump relay.
The fuel pump relay is under the front hood on the Passenger side, inside a cover held on by screws. the fuel pump relay is Blue or light green. The white one is the "main" one. It has to work to make the fuel pump relay to work when starting the car with the key. To test the pump, read diagram on fuel pump relay and use jumper wire to close the switch and if pump works you can hear it running in the trunk at that cover plate.
No You Will Not Be Able To Do This. The Relay Is Required To Make Your Electrical System Work For The Fuel Pump. The Relay Is A Switch Unto Its Self. It Shuts The On And Off As Required By The Fuel System. Even If You Were To Bypass It. The Pump Would Run All The Time. Suggest You Install A New Relay
It should be located on the driver side fender... 2nd relay back (because theres a row of relays in that panel) I heard that you can switch the fuel pump relay with the horn relay and if the vehicle starts and the horn wont work then the relay is bad.
does not have one. that,s right does not have one, if you suspect fuel pump issue check fuel pump relay first.The main engine relay and fuel pump relay work in tandom with each other. Replace in pairs, $ 16.00 ea at kia dealer.Tech bulletin about this.
if it has a roll over switch it may be in the trunk over the fuel tank and it could be a fuse or relay if that doesn't work may be a bad pump.
Bad fuel pump?
yes, replace the relay then check for power, if it doesnt work take out the pump and check it manually by connecting the lead and ground to a battery(watch for sparks, it can ignite the gas in the pump). replace pump if necessary
Are you sure its the relay... Does the car receive spark... if not it might be the ASD Relay located on the finder, under the hood next to the battery, its the last relay there.... the ASD Relay sends power to both coil and fuel pump, if its bad it will cause both not to work.
It does not have 1. If it won't work, then check the fuel pump fuse and relay. You may also have a bad ignition module in the distributor causeing it not to work. Could be a # of different things.
Relay switches are typically in the fuse box compartment underneath the hood usually on the driver's side (~not~ the fuse compartment under the dash) You will see a black plastic box. Pull the cover off. On the under side of cover will have a list of what each fuse, micro relay, and mini relay are for. You will see an entry for the fuel pump relay or possibly just "fuel relay". How to test your relay switch. Some of the relays in the fuse compartment will be exactly the same relay. For instance the horn relay can be (not in all cases)the same type of relay for your fuel pump relay. So if you think you have a bad fuel relay switch... change it with the horn relay switch... then honk your horn. Does it work? (Of course make sure your horn works in the first place). If your horn works with the fuel relay switch substitute... your problem is not with your relay. If your horn does not work.. presto... you have a bad relay. If your relay continues to go burn out... you'll need to find out why. Possibly a bad ground.
Make sure you have voltage at the pump. If you do then the new pump is defective. If not, the fuse is blown or the fuel pump relay is bad. Where is relay?
check the relay first,and then the fuses if this does not work the fuel pump will most likely need replaced
Blown fuse, defective fuel pump relay or the fuel pump has failed.
Chevy does not have a reset switch. If you installed a fuel pump and it still won't work, Then you need to check the wiring harness at the pump where it plugs into the top of the tank. Chevy had a problem with the wire connector at that point.If that checks out okay, then check the fuel pump relay and fuse.
Are you sure you pulled the right relay?
Where is the fuel pump fuse and relay on 1994 BMW 525i and how can I test to see why my fuel pump won't work
Blown fuse, defective fuel pump relay, or the pump itself is defective.
Possibly loose connection or the relay is failing. Replace the fuel pump relay first.
Either the fuel pump itself is defective, the fuse is blown, or the fuel pump relay is defective.
Blown fuse, defective fuel pump relay, fuel filter clogged, or the fuel pump is bad.
On a 1988 Mazda 626, the fuel pump relay is located in the fuel tank. Always disconnect the battery before beginning work on a fuel system.


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THURSDAY, May 10 (HealthDay News) -- A new treatment that involves spinning bone marrow stem cells to enhance their healing potential may help people with advanced heart failure feel and function better, a small study suggests.
Researchers developed the treatment by culturing a patient's own bone marrow for 12 days. This process helped increase the amount of immune cells and stem cells that can differentiate into several different cell types, including heart cells. Those cells were then injected into heart muscle. The study was funded by treatment manufacturer Aastrom Biosciences.
According to the findings, this treatment was safe, helped repair the damaged heart muscle and reversed some heart failure symptoms, when compared to a placebo injection. The findings were to be presented Thursday at the Society for Cardiovascular Angiography and Interventions annual meeting, in Las Vegas.
The U.S. National Heart, Lung, and Blood Institute reports that about 5.8 million people in the United States have heart failure, a condition that occurs when the heart can no longer pump enough blood to meet the body's needs. Symptoms include shortness of breath, fatigue and swelling in the ankles, feet, legs and abdomen. There is no cure; treatment typically includes a cocktail of medications aimed at reducing symptoms and improving quality of life.
"A number of people with heart failure have slowly progressing disease despite medication and/or device therapy. If we could have a therapy for this group that would slow the progression of heart failure, it would be economic and change the disease process tremendously," said study author Dr. Timothy Henry, director of research and an interventional cardiologist at the Minneapolis Heart Institute at Abbott Northwestern Hospital in Minneapolis. The treatment would not be used for people who need a heart transplant.
Calling it the next gener
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domain: health


text:
The Refugee Week theme this year is ‘We Cannot Walk Alone’, and the IMLR is marking it with a guided walk around Bloomsbury following the trail of the 1930s refugees from Nazi Europe. The walk brings to life work by the Research Centre for German & Austrian Exile Studies and uses voices and images from the IMLR’s exile archives to illustrate the stories uncovered by this research. This is the first of two blog posts by Miller Archivist Clare George on the Bloomsbury buildings and institutions that played a role in the history of this community.
The ‘On the Trail of Refugees’ tour traces the many sites which drew the 1930s refugees from Nazi Europe to Bloomsbury. The district’s cheap accommodation, relative to its central location, was one of the main attractions for the new arrivals. The experience of refugee life in one of the district’s low-budget boarding houses was captured by writer Judith Kerr in her semi-autobiographical novel Bombs on Aunt Daisy. The tour takes in the site of the Kerr family’s home from 1935 until the Blitz, the former National Hotel in Bedford Way, immortalised in the novel as the Hotel Continental. Extracts from oral history audio recordings will allow participants to hear other refugees recalling their experiences of staying in Bloomsbury’s boarding houses in the early days of the Second World War.
For the many artists and writers amongst the refugees, Bloomsbury’s Bohemian reputation added to its appeal. The Austrian author Hans Flesch-Brunningen, who was taken by a friend to a boarding house in the district immediately after his arrival in the UK, was one of those who fell under Bloomsbury’s spell. His enchantment stemmed in part from his pleasure at sitting in the British Museum Library ‘under the vast dome of the great reading room … where the exile Karl Marx had sat in the 19th century’. The Library was of course a popular haunt of many other 1930s exiles, who often had little money but plenty of time on their hands since employment options were so limited. Regular refugee users of the Library included anthropologist Ernst Bornemann, political activist Margaret Mynatt, and Austrian author Stefan Zweig, in London from 1934 to 1939, who called it ‘die schoenste Bibliothek der Welt’.
A library of a very different scale, and the opportunity to discuss anti-Nazi activities, brought refugees to 3 Regent Square, in the north of the district. The basement of this house was rented by the political campaigner and pacifist Fritz Gross, who left Germany in 1933 bringing with him his large collection of German-language books. This he used as the basis of a lending library for refugees, gaining official approval from the British authorities to do so in 1935. The flat also served as a meeting point for exiled anti-fascists and other activists, and it was described by Nazi spies at the German Embassy as a central meeting spot for anti-German agitators in London.
Another meeting point for politically-minded refugees thinking ahead to the post-war states to which they would return was the Mary Ward Settlement, which hosted the discussion group ‘Das kommende Österreich’ during 1941. The group stressed the importance of cooperation between all exile groups, but in this case it was the British authorities who were unsettled, suspicious of the group because of its members’ connections with exile Communist groups.
Ten minutes’ walk from the Mary Ward Centre was one of several other Bloomsbury locations associated with the anti-Nazi activists. Socialists Dora Fabian and Mathilde Wurm had left Germany in 1933 to continue in exile with their work to expose the criminal nature of the German regime. They were found dead in the flat they shared in Great Ormond Street in April 1935. The involvement of Nazi agents was suspected by many refugees, and the affair led to extensive discussion in the press and House of Commons about Nazi and anti-Nazi activities in the UK. (The story of Dora Fabian and Mathilde Wurm will be the focus of a talk by Charmian Brinson in October 2021 as part of the Bloomsbury Festival.)
To find out more about this and other sites of historical importance to the refugees, please join us for a free guided tour led by Clare George (Miller Archivist/Research Centre for German & Austrian Exile Studies at the IMLR). Advanced booking is required. Please follow one of the following links to book:
Those who cannot make the guided walks could instead listen to our audio walk, created for the Being Human Festival in November 2020.
Dr Clare George, Miller Archivist/Research Centre for German & Austrian Exile Studies at the IMLR

domain: library


You see the Windows Run dialog with the file name entered. Please note we are carefully scanning all the content on our website for viruses and trojans. Select the printer that you are trying to connect, and then click Next. In the Install From Disk dialog box, click Browse, and then locate the folder to which you extracted the printer driver files. http://wirelessint.com/ibm-infoprint/ibm-infoprint-12-drivers-xp.php
Download Language Size URL Job TicketingMultiple6500000FTP Download package: Download Release date Language Size Download options 5.08 ENU Latest Update4/18/2005English2650000FTP 5.08 PTB Latest Update4/18/2005Brazilian Portuguese2650000FTP 5.08 NLD Latest Update4/18/2005Dutch2650000FTP 5.08 FRA Latest We're commited to providing the best driver to solve your system issues. Learn more or ✘ Close You see the Windows Run dialog with the file name entered. https://answers.microsoft.com/en-us/windows/forum/windows_7-hardware/windows-7-64-bit-print-drivers-for-ibm-infoprint/83e3640c-b6d5-4f48-863e-346318ec8638
Lexmark is constantly tuning and improving performance of their InfoPrint 12 printers and the only way to make use of this is by having the latest drivers. If the cable does meet the specification, the cable itself is marked accordingly. If your printer is connected to a USB port, select the USB port option in the Use the following port list otherwise select LPT.
On the next page, click Local printer attached to this computer. Home -> Infoprint 12 IBM Infoprint 12 driver download Driver Model: Infoprint 12 Downloads: 227 Added: 18 May 2011, 06:27 OS: Windows 7, Windows 8/8.1, Windows 10, Windows XP Are you receiving a printing error message? Starightfoward and intuitive interface Better PC performance and improved stability Access to the largest drivers database in the industry Continous updates for the latest manufacturer drivers Unlimited free technical supports with
Pages Home Contact Us Data Extraction | Contact | Accessibility | Products | Disclaimer | CSS and XHTML Copyright © Driver-Download.Net 2007-2012. Should you choose not to save the application, you may simply initiate the free scan by clicking the "Run" button on the File Download Window. your help is greatly appreciated! [close window] thank you for your feedback thank you! https://us.driverscollection.com/?H=Infoprint%2012&By=IBM When your InfoPrint printer is relatively new, it may be possible to download individual drivers directly from Lexmark without incurring additional charges.
Follow the instructions at the top of each page of the wizard. Test the printer driver To test the printer driver, see whether you can print from WordPad or Notepad. On this page, click Have Disk. Windows 8 (32 and 64-bit) Windows 7 (32 and 64-bit) Windows Vista (32 and 64-bit) Windows XP (32 and 64-bit) Windows Server 2003 Windows Server 2008 Windows 2000 Acer Alienware ASUS
If you are prompted to use an existing driver, select Replace existing driver, and then click Next. Follow the instructions at the top of each page of the wizard. Click Next. We'd appreciate if you'd contribute to this project by sending us even more drivers to include in our library.
The Install From Disk dialog box opens. weblink The Add Printer Wizard opens. Test the parallel port driver and the hardware If the printer is connected to an LPT port, see whether you can print from a command prompt. When using a company's driver you will bound to that company's own legal agreement.
You may have to do this more than once to fill the print buffer on some printers. The printer drivers work with these IBM printers: - IBM Infoprint 2060ES - IBM Infoprint 2075ES - IBM Infoprint 2085 - IBM Infoprint 2090ES - IBM Infoprint 2105 - IBM Infoprint If you were unable to complete the above steps to install a printer driver, or if you still have problems printing, you might have to ask someone for help or contact navigate here Comments Tweet Add comment Driver problems?
See whether the printer cable meets the IEEE 1284 specification. For example, create a new document that contains less information. Should you have any driver-related technical questions, please feel free to ask them on our forum.
If your printer is connected to a USB port, select the USB port option in the Use the following port list otherwise select LPT. It's very important you know the exact model of your Lexmark InfoPrint 12 printer you would like to update to ensure that it will function properly and to prevent possible damage No affiliation or endorsement is intended or implied. please let us know so we can remove the driver.
Click Share name, and then specify a name for the shared printer. The "Printers and Faxes" window opens. Page-oriented printers (for example, laser printers) generally do not start to print until a form feed PCL command is sent or until more information than just a page is present in his comment is here couldn't you download the driver ?
The IBM TCP/IP port monitor work with these IBM printers: - IBM Infoprint 2060ES - IBM Infoprint 2075ES - IBM Infoprint 2085 - IBM Infoprint 2090ES - IBM Infoprint 2105 - To update the printer driver on Windows XP manually, follow the steps below or click here to let us fix it automatically: Click Start, and then click Run. You see the Windows Run dialog. Unfortunately, if the test page was not printed successfully, you still have some troubleshooting to do that is beyond the scope of this article.
Device: Any device Bios Cameras Game Devices Input Devices Laptops - Desktops Modems Motherboards Network Adapters Other Printers Projectors Scanners Sound Cards USB Video Adapters »Home»Printers»IBM»Infoprint 12 Drivers System Issues? If the output is printed to the printer from the dir command, the print driver or printer configuration is probably the source of the problem. Check the available space on the hard disk. Click Next.
Click "Run" ("Save As") if using Firefox Click "Run" Again Follow on-screen prompts to complete installation.


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May Cheese Subscription – Autumn selection
Each month we choose a selection of cheeses with a common theme that are eating particularly well. Our May Cheese Subscription Box celebrates the arrival of Autumn.
Autumn brings cooler mornings, the changing colours of the leaves and the arrival of the local mushroom season. These cheeses are perfectly matched with silky soft flavourful mushrooms, or as part of an autumn cheese board, with your favourite seasonal vegetables and a glass of something special.
PARMIGIANO REGGIANO DOP – G.Cravero
Cow’s milk, Modena, Emilia-Romagna region, Italy
Selected by Giorgio Cravero for sale in Australia at approximately two years of age, this outstanding example has a succulent, moist texture with nutty notes and a complex, fruity sweetness that makes it an ideal table cheese.
G.Cravero Reggiano is delicious crumbled and enjoyed with Prosecco or shaved over your favourite mushroom dish.
TALEGGIO – Mauri Formaggi
Mauri Taleggio DOP is a classic Italian washed rind cheese with a creamy texture. Washed and brushed several times over a month, and matured in stacked wooden pine boxes, the cheese develops a thin bloom flecked with grey yeasts and blue mould on its distinctive orange rind.
BOSCHETTO AL TARTUFO – Il Forteto
Il Forteto Boschetto al Tartufo is a fresh, young pecorino-style cheese made with a combination of rich, creamy ewes milk and full-cream cows milk. This cheese is infused with prized fresh white truffles from the surrounding Tuscan countryside. Dense and moist in texture, this is a deliciously aromatic cheese.
ST DAVID DAIRY CULTURED BUTTER
Cow’s milk, Fitzroy, Victoria
St David Dairy fine cultured butter is handcrafted in small batches at the Fitzroy micro-dairy using only the freshest cream and premium cultures. It is slow-fermented, batch churned, and hand salted using Mount Zero Fine Pink Lake Salt Crystals. It adds another dimension to pan-fried mushrooms or your regular breakfast toast.
Order your cheese subscription today
Orders for May close at 9 am Friday 24 April. For delivery Friday 1st May (Thursday 30th April in some regional areas). See more information and order here.
Perfect extra accessories to add to your May subscription
Opinel Mushroom Knife No 8 is the ideal knife for mushroom foraging or the perfect gift for your mushroom loving foodie mates.

domain: food


text:
Wellington, himself an old Etonian, is often misquoted as saying that the battle of Waterloo was won on the playing fields of Eton.(1) It is strange that Harrow, Eton's strongest rival, has produced three of the biggest admirers of Wellington's opponent at Waterloo!
The David picture above, some seven feet high, was commissioned in 1811 by the first of our old Harrovians, Alexander Hamilton, 10th Duke of Hamilton (1767-1852). Hamilton, a Scottish Whig politician, apparently had a tendency to emphasise the importance of ancient birth, but this curiously did not prevent him from supporting Napoleon, an upstart whose career and credo symbolised equality of opportunity. In 1882 the painting that Hamilton had commissioned was acquired by Lord Rosebery, like Hamilton a Scottish politician of Whig ancestry.
A Liberal Imperialist who briefly served as Foreign Secretary twice, and then Prime Minister(1894-1895), Rosebery's marriage to Hannah Rothschild (1878) enabled him to buy a priceless collection of art, including a number of valuable Napoleon memorabilia, to fill his twelve houses. (2) Rosebery was the author of an excellent study of Napoleon's exile which is still worth reading, and also commissioned the famous portrait of Napoleon by James Sant.
Rosebery was an old Etonian, but whilst the David painting was in his possession it caught the admiring gaze of the second of our old Harrovians, the young Conservative politician, Winston Churchill, a passionate devotee of Napoleon.
This was the first time that Churchill had been confronted by a large life-size picture of his hero. It made such an impression on him that the very next day he wrote to Rosebery about it:
I carried away quite a queer sensation from the Napoleon picture yesterday. It seems pervaded with his personality; and I felt as if I had looked furtively into the very room where he was working, and only just got out of the way to avoid being seen. (3)
I have written a number of posts about Winston Churchill's intense admiration for Napoleon, and a recent reading of Michael Sheldon's Young Titan, The Making of Winston Churchill, the only biography which has focused on his early political career, has shed further light on this:
In 1912, now a Liberal and First Lord of the Admiralty, travelling by train across the Alps to Naples to try to persuade the retired Lord Fisher to help the Government with its naval plans, Churchill waxed so eloquently about Napoleon's crossing the Alps that his wife in the next compartment thought he was reading aloud. (4)
In April 1915, critical of the performance of the Navy in the early stages of the ill-fated Gallipoli campaign he quoted Napoleon to Admiral Fisher,
'We are defeated at sea because our Admirals have learned--where I know not--that war can be made without running risks' [Napoleon] (5)
Contemporaries were of course well aware of his passion for Napoleon. "He thinks himself Napoleon " said Lord Esher, his superior at the Colonial Office. His friend John Morley, Gladstone's biographer, when told that Churchill was reading another book on Napoleon commented,
He would do better to study the drab heroes of life. Framing oneself upon Napoleon has proved a danger to many a man before him. (6)
Perhaps the strongest criticism came from a political opponent, Admiral Lord Charles Beresford who described him in a Unionist rally in Hyde Park in 1914 as an enemy of Ulster and a danger to the State, a "Lilliput Napoleon. A man with an unbalanced mind. An egomaniac ..!" (7)
The third of our old Harrovian aristocratic admirers of Napoleon was of course Lord Byron, from whom Churchill drew great inspiration. Both Byron and Churchill kept busts of Napoleon on their desks. Byron seemed in Churchill's mind to resemble his own father whose career had been tragically cut short, and to possess that combination of energy, action, vision and free thinking which he so admired.
Shelden's study suggests that as a young man Churchill very much saw himself as a Byronic romantic hero, a protagonist of domestic reform, determined to change the world for the better, and one whose political career looked to have come to an end with the failure of the Gallipoli Campaign. Churchill though did not perhaps quite look the part, and the book throws new light on his passionate if unsuccessful pursuit of three of the most beautiful women of his time. (8)
Churchill was a great lover of Byron's poetry and could recite long passages from it, and often drew on it as in his famous promise in 1940 of only "Blood Sweat and Tears" which echoed Byron's reference in his Age of Bronze to the war profiteering of the landed gentry who enriched themselves with their "blood, sweat and tear-rung millions". (9)
1. Others have claimed that what he actually said decades later when observing an Eton cricket match was "There grows the stuff that won Waterloo".
2. The David painting was bought by the Samuel H. Kress Foundation in 1954 and now hangs in the National Gallery of Art in Washington D.C.
3. Churchill to Rosebery, 4th Sept 1901 p. 45 Michael Shelden, Young Titan, The Making of Winston Churchill, Great Britain, 2014.
4. Shelden pp. 278-9.
5. Winston, Churchill to Admiral Lord Fisher, April 8th 1815. Churchill Archives.
6. Shelden p. 92
7. Shelden pp. 195, 270.
8. Shelden pp 7-10.
9. Apparently when in 1941 Franklin Roosevelt suggested that the allies should call themselves the United Nations Churchill immediately quoted a verse from Byron, "Here, where the sword united nations drew .." Shelden pp 6-7.

domain: artistic


text:
Chinese Vice-Premier Liu He (left) meeting US Treasury Secretary Steven Mnuchin and US Trade Representative Robert Lighthizer outside the US Trade Representative office in Washington on May 9. Photo by Reuters
THIS week’s overnight policy rate (OPR) cut of 25 basis points by Bank Negara Malaysia met the expectations of most economists as concerns over stalling growth rise.
The rate cut is an indication of moderating growth, with the central bank’s move seen by many as a way to stimulate economic activities when borrowing becomes slightly cheaper for corporations and individuals.
Even so, US President Donald Trump appears to have further muddied the waters of global trade last week by imposing a 25% tariff hike on US$200 billion (RM832.24 billion) worth of Chinese imports. For Malaysia, this could negate the many benefits of the OPR cut.
The main concern was that if the talks fail, the US will impose more tariffs on the remaining imports from China, or a US$290 billion worth.
“A further deterioration in trade tensions will risk derailing the expected pickup in the global economy in the second half of the year. Global central banks will likely cut interest rates, especially the US Federal Reserves, to stem the US economy from slowing down sharply,” Socio-economic Research Centre executive director Lee Heng Guie says.
In the Asean region, Malaysia was noticeably the first to cut its key rate, but it was second in Asia after India. After Malaysia’s OPR rate reduction to 3% from 3.25% on May 7, the Philippines followed suit on May 9 by paring its benchmark rate to 4.5% from 4.75%.
In a report, MIDF Research economist Dr Kamaruddin Mohd Nor said Bank Negara’s rate reduction should bring about improvement in investment and domestic consumption activities in the second half of the year.
However, Lee cautions that monetary policy alone will not support the economy.
“It must be complemented by a mix of supportive fiscal and growth policies. These include the ongoing construction of public transportation projects (LRT3 and MRT2), accelerated disbursement of the Budget’s development expenditure as well as the revived East Coast Rail Link (ECRL) and Bandar Malaysia.
“With a still-supportive environment for consumption, the quantum of rate cuts is deemed sufficient to provide some support to consumer spending — although there will be some lag time to its impact — along with continued wage growth, cost of living aid, a stable labour market, stable fuel prices and subdued inflation,” he says.
Lee expects the domestic economy to bottom out in the second quarter. Thereafter, it will moderately improve in the year’s second half, assuming that the global economy will pick up due to more growth-friendly policies adopted by central banks.
However, the ongoing trade tension between the US and China could throw a spanner in the works of improving global economic growth.
The two economic superpowers were expected to resume another day of negotiations on May 10. There was still hope that the US and China could end the week with an agreeable deal, though many believe the risk of it falling through has increased.
In a scenario where the remaining Chinese imports are also slapped with tariffs and European auto imports suffer the same fate, US gross domestic product (GDP) growth will fall into negative territory, notes Deutsche Bank.
Subsequently, the eurozone will fall into a recession while China would also see slower growth, which will severely impact Asian economies, it bank adds.
“Initially, slower export growth would likely be combined with a withdrawal of foreign portfolio capital, putting downward pressure on currency exchange rates to the dollar. But weaker currencies would depress import demand over and above the decline in imports stemming from the contraction of export-oriented activities.
“The current account balances would start rising, which will eventually end the sell-off in Asian currencies. Countries entering this scenario with current account deficits may take longer to stabilise and may need to raise interest rates to help this process along,” it explains.
Like SERC’s Lee, Deutsche Bank expects the Fed to cut interest rates in a full-blown trade war scenario. It adds that most economies will introduce monetary stimulus measures, as the Fed cut rates to zero and possibly resume quantitative easing.
That said, the basis for economists is that the two parties will reach some kind of resolution this year.
For now, they are not expecting to see another rate cut for this year. AmBank Research believes the likelihood of a rate cut will depend on the severity of the downside risks to growth and whether there is evidence of tightening financial conditions after the latest cut.
Lee says the 25bps cut is expected to be a one-off policy rate easing as Bank Negara would reserve its monetary arsenal for future economic shocks.
“While the domestic economy is slowing, it is not in (such) dire straits to warrant a big dose of monetary easing. Bank Negara has many policy tools to choose from before wielding blunt instruments such as interest rate cuts. These include reduction in the statutory reserve requirement to boost credit growth and reduce firms’ borrowing costs,” he says.

domain: news


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Marin County Sheriff Robert Doyle has been sued for illegally sharing millions of local drivers’ license plates and location data, captured by a network of cameras his office uses, with hundreds of federal and out-of-state agencies, such as Immigration and Customs Enforcement (ICE), Customs and Border Protection (CBP), over a dozen other federal law enforcement agencies, and more than 400 out-of-state law enforcement agencies.
This practice has violated two California laws, endangers the safety and privacy of local immigrant communities, and facilitates location tracking by police.
The suit seeks to end the sheriff’s illegal practice of giving hundreds of agencies outside California access to a database of license plate scans used to identify and track people, revealing where they live and work, when they visit friends or drop their kids at school, and when they attend religious services or protests.
The lawsuit was filed in Marin County Superior Court by the ACLU Foundations of Northern California, Southern California, and San Diego and Imperial Counties, the Electronic Frontier Foundation (EFF), and attorney Michael T. Risher representing community activists Lisa Bennett, Cesar S. Lagleva, and Tara Evans, who are longtime Marin community members.
License plate scans occur through Automated License Plate Readers (ALPRs): high-speed cameras mounted in a fixed location or atop police cars moving through the community that automatically capture all license plates that come into view, recording the exact location, date, and time that the vehicle passes by.
The Marin County Sheriff’s Office scans tens of thousands of license plates each month with its ALPR system. That sensitive personal information, which includes photographs of the vehicle and sometimes its driver and passengers, is stored in a database.
The sheriff permits hundreds of out-of-state agencies and several federal entities, including the Department of Homeland Security, to run queries of a license plate against information the sheriff has collected. The agencies are also able to compare their own bulk lists of vehicle license plates of interest, known as “hot lists,” against the ALPR information collected by the sheriff’s office.
“In the hands of police, the use of ALPR technology is a threat to privacy and civil liberties, especially for immigrants. Federal immigration agencies routinely access and use ALPR information to locate, detain, and deport immigrants. The sheriff’s own records show that Sheriff Doyle is sharing ALPR information with two of the most rogue agencies in the federal government: ICE and CBP,” said Vasudha Talla, immigrants’ rights program director at the ACLU Foundation of Northern California. “Police should not be purchasing surveillance technology, let alone facilitating the deportation and incarceration of our immigrant communities.”
California’s S.B. 34, enacted in 2015, bars this practice. The law requires agencies that use ALPR technology to implement policies to protect privacy and civil liberties, and specifically prohibits police from sharing ALPR data with entities outside of California.
The sheriff also violates the California Values Act (S.B. 54), also known as California’s “sanctuary” law. Enacted in 2018, the law limits the use of local resources to assist federal immigration enforcement.
“The information unveiled through this lawsuit shows that the freedoms that people think they possess in Marin County are a mirage: people cannot move about freely without being surveilled,” said Bennett. “Our county sheriff, who has sworn to uphold the law, is in fact violating it by sharing peoples’ private information with outside agencies. This has especially alarming implications for immigrants and people of color: two communities that are traditionally the targets of excessive policing, surveillance, and separation from loved ones and community through incarceration or deportation.”
The Marin County Post’s coverage of local news in Marin County is supported by the Ethnic Media Sustainability Initiative, a program created by California Black Media and Ethnic Media Services to support community newspapers across California.

domain: sociology


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Long range relationships increasingly becoming popular today. Statistics showed that around 14 million people in the United States only consider themselves to be within a long distance relationship(in 2021). This shape was almost the same as the earlier years with an estimated quote of about 14 mil. Moreover, of that 14 million, practically four to five mil of them are previously in a pre-marital relation.
Distance has always been a problem when it comes to long relationships. But with the widespread using of communication technology like the net and cell phones, this problem is made worse. It may not seem that big of a factor, as couples may have their personal lives collectively and discuss on the phone in cases where needed. But the fact of the matter is, not everybody uses the internet or perhaps owns cellular phones. That is the reason for what reason the elements come into perform.
One of the most common factors in all long-distance romantic relationships is usually geographical closeness. In this sort of relationship, south korean mail order brides partners should be geographically close enough to be able to communicate with each other regularly. Of course , the majority of couples aren’t physically close to each other all the time. But they has to be close enough to be able to get together regularly, regardless if that just simply involves checking out your email for the day or perhaps catching on some media.
Communication solutions have also brought along various forms of geographic closeness. For example , a lot of long length couples are now trying to stay in touch through mobile phones. In some cases, that they meet up once a week or even more frequently depending on what lengths apart they will live. A second form of communication technologies which includes brought along more closeness is a use of Skype, Google+ Local, and Bluetooth.
And after that, there are the non-commemorative elements that make up the majority of long-distance human relationships. One of the common elements is the age difference between the two partners. Since it’s difficult to meet up literally when you are certainly not geographically close, the old partner usually takes care of arranging for transportation. Nevertheless they still want someone to text message or call if they need to keep in touch, which is in which the age gap is supplied. It means a younger person might be using a newer cellular telephone than his / her partner, which makes it even more important to the younger person to be logged on to the internet at all times.
So , the next question is, as to why would anyone want to do such a study? Well, should you or your partner has had past long distance romances, you may already know the dynamics that come with those varieties of relationships. Of course, if you’re just starting out, you know that the dynamics of the people kinds of interactions are totally different. So , an additional reason to log onto the net, and do some back to top rated, click here!


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domain: hobby


The Twins won their first game of the season last night as their bats defeated Jared Weaver, the Los Angeles Angels, and the Twins own defense by a score of 6-5.
It was a victory not just for the players on the field, but for the front office and coaching staff, as it finally validated decisions they had made throughout the offseason and spring training. Those decisions, contrary to the popular view of the Twins as a fundamentally sound team, have emphasized scoring runs over solid
Twins get their first MLB win, overall a 4-1 day.
Please note, I am NOT trying to step on Seth's toes at all by the day-by-days, he adds good narrative, and he knows a HELLUVA lot more than I, or anyone not employed by the Twins do about the Minor League. This blog is meant just to be used as a nice snapshot of the system on a day-by-day basis (see what I did there?). So use them as you will, I plan on adding stats starting next week, but rely on Seth for his knowledge and recaps!
Whew... glad to get that out of the way. This is how the Twin's are going to have to perform to win games. We will need to score 5+ runs for the most part to win a game, but at least Carl gave us some innings. The inside job by Bourjos was a knee to the nuggets, but what the heck, it all worked out. Kudos to Willingham and Parmelee for their big hits. I have been an opponent of the Willingham signing, not because I don't like him or his contract, but more so for philosophical reasons on how I think
The Twins got Win #1 in Game #5, and that has to be a relief to all the players, coaches, the manager and the front office. As a fan, itís kind of nice as well! A very nice 6-5, come-from-behind win. At various times, Josh Willingham, Chris Parmelee, Jamey Carroll, Glen Perkins and Matt Capps played hero. Jeff Gray gets the win! Congrats to the Twins on the win.
The minor league affiliates won three of their four games on Wednesday. In unusual fashion, two of the Twins affiliates played games
Updated 04-11-2012 at 10:11 PM by Seth Stohs
[Originally posted at Twins Fan From Afar]
Will this man-child ever throw for the Twins again?
There's no good news report today, as it was just announced that our best righty, Scott Baker, will miss the entire season
this post was originally posted at http://troystwinsdugout.wordpress.co...in-the-mirror/
The Twins haven't done well to start the season. They're 0-4 after losing three straight to The Baltomore Orioles and the home opener against the Angels, scoring only 6 runs compared with 20 runs allowed.
Now that they've had an off day to assess where they're at, I think it will be interesting to find out how they will respond. Granted,
Updated 04-11-2012 at 06:21 PM by Troy Larson
Per La Velle.
This doesn't sound good. I'll hope for the best, but it's tough to imagine a press conference with Terry Ryan sitting next to Baker, and Baker saying "I'm 100% ready for action!"
Here's my first shot at this. I'm assuming no one will see it, but if you do, Thanks....and sorry. I was going to post something earlier with predictions and such but then remembered I hate predictions and such. So I decided to give it a few games before I chimed in.
I have to go at this from the only perspective I have, from the view of a fan 4 hours away. So far its pretty simple: This is bad. It hurts to watch on TV. I half expect Bert to jump from the booth to his werciful demise.
Thus far, good things are coming out of New Britain
[Originally published at Twins Fan From Afar]
A few unrelated items this morning. First, if you missed it, yesterday I wrote about Alex Wimmers' first AA Rock Cats start that took place Monday night,
Updated 04-11-2012 at 08:25 AM by Twins Fan From Afar
Original post from North Dakota Twins Fan
One of highlights of Opening Day at Target Field was the 10-year reunion for the 2002 Minnesota Twins team, the last team from the franchise to play in the ALCS. There were 11 of the 25 members of the team in attendance at the game along with most of the coaching staff. There were plenty of cheers for fan
Updated 04-11-2012 at 07:39 AM by Cody Christie
originally posted at ontheroadwithshawn and TwinsTarget.
7. Aaron Hicks, Center field, 22 years old
2011 Stats: .242/.354/.368 with 5 HRs and 17 SB in 26 attempts for Fort Myers.
Last Year's Rank: 2
Acquired: Drafted by the Minnesota Twins 14th
Aaron and John share some exciting news about the podcast and the unexciting news surrounding the Twins 0-4 start. Here are:
the podcaststhe rss feed if you want to subscribe andthe podcast on iTunes (where you can also subscribe and leave reviews)our new sponsors: KFAN & Pickpointz.
Let's say Joey V. gets the axe. Who takes over his position? Is Bruno called up from AAA? Or would it be Scott "Weirdo" Ulger to assume that position? I assume the latter, although I would be pro for the former. What's your take?
On Monday against the Angels, Nick Blackburn, despite taking the loss in a 5-1 contest, provided the team with some assurance that he may be able to outperform the lowly expectations that have been outlined for him based on his track record the last two years.
What has been the most impressive about his 2012 debut was his ability to miss bats. After all, at an 89.9% rate over the past two seasons, he has allowed more contact than any other qualified starting pitcher. It has been


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Clin Microbiol Infect 2012; 18: 1168–1175
Microbiological culture is the conventional method for establishing the diagnosis in implant-associated bone and joint infection, but it may lack both specificity and sensitivity. Molecular diagnosis has been an important step in the diagnosis of infectious diseases. We review the principles and the role of molecular diagnosis in improving the aetiological diagnosis of implant-associated bone and joint infection. Currently, molecular diagnosis mainly includes conventional broad-range PCR and specific PCR assays. These tools are efficient, but several pitfalls exist that necessitate rigour in all steps of the process. In implant-associated bone and joint infection, molecular assays have been shown to be useful in complementing culture techniques to identify microorganisms when patients have previously received antibiotics or in the presence of fastidious microorganisms. Broad-range PCR targeting the 16S rRNA sequence followed by sequencing must be performed in culture-negative specimens when infection is suspected on the basis of clinical signs and symptoms or inflammatory syndrome. This molecular tool has allowed not only increasing identification of anaerobic bacteria, such as Finegoldia magna, but also the discovery of the role of Tropheryma whipplei, an aetiological agent of implant-associated bone and joint infection in patients without Whipple’s disease. Real-time pathogen-specific PCR assays performed in a closed system are more sensitive and specific than broad-range PCR, but each assay is typically able to detect only a single microorganism. These assays should be performed to confirm the identification provided by broad-spectrum PCR, and also when broad-range PCR fails to detect a microorganism despite efficient DNA extraction.
The diagnosis and treatment of implant-associated bone and joint infection is a major challenge . These infections are mainly caused by microorganisms that grow in biofilms on the surface of the implant . In the biofilm, microorganisms are difficult to diagnose and treat effectively. Indeed, they are recalcitrant to conventional antimicrobial agents and host immune responses . The failure of aetiological diagnosis with traditional culture may be caused by prior antimicrobial exposure and fastidious microorganisms, but it is also possibly related to the reduced growth rate of biofilm microorganisms delaying or inhibiting growth in microbiological culture [1,2].
In culture-negative cases, treatment is performed on an empirical basis, with poor prognosis [1,3]. Molecular tools may help to establish an aetiological diagnosis, but molecular procedures must be rigorously performed because of the risks of interfering contamination . The efficiency of PCR assays in detecting microorganisms depends on sample collection, PCR methodology, validation, and the interpretation of each PCR analysis . Basic rules must be applied to obtain reliable results. In this article, we review the entire process of performing and interpreting molecular amplification assays, and their role in the diagnosis of implant-associated bone and joint infections.
Clinical Samples for PCR Assays
PCR assays should be performed on fluids collected by needle aspiration or on periprosthetic tissues obtained by surgical biopsy . At least three specimens should be sampled for culture [3,6–8]. They should be shipped immediately to the laboratory after collection . Prior to culture, a piece of each specimen should be stored at −20°C or −80°C for further analyses [5,9]. If fresh specimens are not available, PCR could be performed on paraffin-embedded biopsy specimens, but this alternative lacks both sensitivity and specificity . Superficial wounds should be avoided, because of the frequent colonization by microbial flora from the surrounding skin . Samples collected by swab should be avoided, as they may lack sensitivity . The application to the specimens of an appropriate sonication technique with an ultrasound bath has recently shown encouraging results .
Prior to molecular amplification, DNA extraction is an important step. Various protocols have been reported [5,9,13–16]. To optimize the quality of the DNA extracts, an overnight step in lysis buffer and proteinase K before extraction is strongly advised for bone and joint specimens, including fluids. Moreover, the use of automation, which allows the elimination of several manual steps and the risk of cross-contamination, should be preferred.
Interpretation of PCR Assays
PCR pitfalls and solutions
There is sometimes a lack of efficient DNA extraction, leading to false-negative results. The main critical points are the technique used and the cell wall of the bacteria . The quality of DNA extracts must be evaluated by amplifying a fragment of a human gene, such as the β-globin or actin gene [5,9]. In cases of negativity, another specimen must be extracted at another time. The amount of background DNA from bacteria and the size and abundance of the amplified fragment may also play a role [17,18]. When possible, a short target (c. 100 bp) should be designed .
Molecular assays are also prone to contamination, which can occur from the time when the samples are obtained to their manipulation during the laboratory process [4,19]. Contamination may occur from one sample to another during the assay, or from laboratory surfaces, tubes, pipettes, the technician’s hair or clothes, or previous amplicons in the laboratory. Even if those risks can be lowered by following strict rules, single-use plasticware, tubes, water and reagents can still be a source of contamination [4,20,21]. Thus, it is imperative to carefully follow standard recommendations to prevent contamination (Fig. 1) [5,21,22].
The presence of positive and negative controls in each PCR assay is necessary to validate the assay. The use of positive controls (extracted bacterial DNA) allows confirmation that the PCR process was correct. DNA from a microorganism that does not commonly cause implant-associated bone and joint infection, such as Escherichia coli, is preferred to DNA from a common causative pathogen. The use of negative controls, processed from DNA extraction to PCR in parallel with the tested samples, is imperative to detect contamination. Water, a mixture of all reagents used in the PCR assay and DNA extracted from human tissue without infection can be used as negative controls .
Both negative and positive controls must be correct for interpretation of the PCR. If the positive control was not detected or if any amplification of the negative control occurred, the run is unreliable  and should be repeated. For broad-range PCR, each positive amplicon must be sequenced for accurate identification of the causative microorganism. An original sequence observed for the first time in a laboratory can usually be considered to be a true positive . A similar sequence found in the same run from samples of two patients suggests potential contamination. Sequences from microorganisms that are commonly present in water or reagents (Pseudomonas spp. and Acinetobacter spp.) and those from microorganisms from skin (coagulase-negative staphylococci and Propionibacterium acnes) may result from contamination. The interpretation of the results is sometimes difficult, as coagulase-negative staphylococci are the most commonly observed pathogens in implant-associated infections. Thus, the microorganisms of the normal skin flora may be either contaminants or pathogens. If a result is doubtful or has a low predictive value, supportive data can help in the interpretation. PCR targeting a second gene can be performed with the same DNA extract, and another sample of the patient can be extracted and tested at another time.
Finally, the persistence of DNA from the causative agent has been reported in infective endocarditis from months to years after clinical cure. It is possible that, as with endocarditis, the link between the current episode and the amplified DNA needs to be evaluated for implant-associated bone and joint infections [24,25]. However, to the best of our knowledge, there are currently no published data on this potential diagnostic problem.
Usefulness of PCR Assays
The main indication for PCR assays is the lack of microorganism culture. No microorganisms are detected in approximately 5–15% of apparent infections . However, suspicion of mixed infections, mainly in the case of open lesions, can be also an indication.
Molecular diagnosis has allowed spectacular data to be obtained for the identification of Kingella kingae among children with osteoarticular infections [13,15,26]. Currently, for patients with implant-associated bone and joint infections, PCR assays have mainly allowed the identification of Granulicatella spp., Staphylococcus aureus, even in cases of small-colony variant infection, and anaerobic bacteria [1,27]. Indeed, the implication of Finegoldia magna as a pathogen in prosthetic joint infection has been rediscovered by broad-spectrum PCR [28,29]. PCR has also allowed the establishment of the role of Tropheryma whipplei as an aetiological agent of prosthetic joint infection without other classic signs of Whipple’s disease [30–32]. More recently, Legionella micadadei has been reported to cause prosthetic joint infection, thanks to the systematic use of 16S rRNA PCR . PCR could also help in the diagnosis of other fastidious microorganisms involved in implant-associated bone and joint infections, such as Mycobacterium tuberculosis or other Mycobacterium spp. [34–36].Thus, broad-spectrum PCR is useful in the diagnosis of difficult-to-culture organisms, especially when the pathogen is rare or unsuspected.
Previous antibiotic therapy
Antimicrobial therapy must be discontinued at least 2 weeks before the sampling of specimens . In cases of revision surgery, perioperative prophylaxis must not be administered until after tissue specimens have been collected. However, in cases of a longer duration of antibiotic therapy or inadequate prophylaxis, broad-range PCR may help to establish an aetiological diagnosis [5,22,37].
The potential inability of broad-range PCR to identify a mixture of bacterial species in a single specimen has been considered . However, 16S rRNA PCR combined with cloning is able to detect mixed polymicrobial infections, allowing for identification of the main bacteria present in the mixture . Moreover, with this approach, new potential microorganisms have been reported among patients with mixed infections, whereas no novel bacterial species were detected in monobacterial specimens . These unusual microorganisms are not detected in culture, mainly because of overgrowth by rapidly growing species.
Conventional Broad-range PCR
The 16S rRNA gene, the 23S rRNA gene, the rpoB gene and the 16S–23S intergenic spacer are present in all bacteria, and are thus particularly suitable for broad-range PCR . Several protocols have been reported for 16S rRNA gene-based detection of bacteria [16,19,39–41]. However, only 16S rDNA amplification, systematically followed, when positive, by sequencing , allows accurate identification of the involved microorganisms, and must be used (Fig. 2). Moreover, nested or semi-nested PCRs that correspond to the reamplification of a PCR product must be avoided, owing to the high risk of contamination [32,42]. A polymicrobial infection must be suspected if a mixed sequence is observed on the DNA pherogram, and a cloning step with commercialized kits should be performed . Sequencing of all of the clones is time-consuming, but it may help to identify new pathogens. Indeed, a sequence similarity of <97% of the 16S rRNA sequence is the criterion used to define a potentially new bacterial species [43–45]. The main primers that have been published for the diagnosis of implant-associated bone and joint infections are summarized in Table 1 [5,9].
|References||Microorganisms||Targeted sequences||Forward primer: name and sequence (5′–3′)||Reverse primer: name and sequence (5′–3′)|
|Fenollar et al. ||Eubacteria||16S rRNA||536F, CAGCAGCCGCGGTAATAC||rp2, ACGGCTACCTTGTTACGACTT|
|Fihman et al. ||Eubacteria||16S rRNA||536f, CAGCAGCCGCGGTAATAC||1050r, CACGAGCTGACGACA|
|Eubacteria||16S rRNA||16S-241bp-F, GGAGGAAGGTGGGGATGACG||16S-241bp-R, ATGGTGTGACGGGCGGTGTG|
|Eubacteria||16S rRNA||16S-cons-F, YGGCGRACGGGTGAGTAA||16S-GP-R, CCGATCACCCTCTCAGGTCG for GP |
16S-GN-R, AGTTAGCCGGTGCTTCTTCT for GN
|Fenollar et al. ||Staphylococcus aureus||RpoB||Saur.Rpob.F, GTTTGAATTGCATGGTAGCGT||SaurRpob.R, GAAGCAATGATATCTGCTGGTG|
|Streptococcus group B||RpoB||SagaRpob.F, CAATTGCAGAGCATATCGATGG||SagaRpob.R, TCCAACAATAGTAACAACACGG|
|Streptococcus pneumoniae||RpoB||SpncuRpob.F, GGTAGAAGCTGGTACGATTATGAC||SpneuRpob.R, GATCAGTTGGAGCAACAACCT|
|Enterococcus spp.||RpoB||StrpF, AARYTIGGMCCTGAAGAAAT||StrpR, TGIARTTTRTCATCAACCATGTG|
|Granulicatella adiacens||RpoB||GadiaRpob.F, TGTAACTCTAACACTTGTCCGA||GadiaRpob.R, GGACGTCACGGTAATAAAGGG|
|Staphylococcus epidermidis||RpoB||SepiRpob.F, GTGATACGTCCATGTAATCCA||SepiRpob.R, TTTGACAGCTGATGAAGAGGA|
|Enterobacteriaceae||RpoB||CM7, AACCAGTTCCGCGTTGGCCTGG||CM31b, CCTGAACAACACGCTCGGA|
|Escherichia coli||RpoB||EcolRpob.F 5′-TTCACCAACGATCTGGATCAC||EcolRpob.R 5′-GAAGAACAGGTTCTCGAACAG|
|Pseudomonas aeruginosa||RpoB||PaerRpob.F, TGTACACCAACGACATCGAC||PaerRpob.R, AAGAACAGGTTGCCGAACAG|
|Clostridium perfringens||Eabcc||CloPerF, GACACATTAGTTGGGAAGTG||CloPerR, GTTGTTAACCAATGAGCAGC|
|Propionibacterium acnes||16S–23S rRNA||PacnF, CTAAGGAGTTTTTGTGAGTGG||PacnR, CTTTGCACAACACCACGTC|
|Prevotella spp.||CfxA2-like lactamase||PrevoF, GGATAAACTTGACCCAAAGAC||PrevoR, GATGTATAGTTAGAGTAAGCC|
Limits of conventional broad-range PCR
Although most studies agree about the good specificity of broad-range PCR in the diagnosis of implant-associated bone and joint infections, discrepancies have been reported about its sensitivity, with several studies showing poor sensitivity (<50%) [5,9,40,46–48]. Several biases could have influenced these data, such as the lack of pretreatment prior to DNA extraction, the lack of control of the extracted DNA quality, or the small number of studied patients . Among the three studies that have involved more than 50 patients, the sensitivity of 16S rRNA PCR for osteoarticular infections (both prosthetic and not) was determined to be 92.5% vs. 89% for culture  in the first study. The second study reported a PCR sensitivity of 73.3% (53.8% for prosthetic joint infections and 88.2% for infections without prostheses), whereas for cultures, the sensitivity was 96.7% . In the third study on prosthetic joint infections, culture and PCR had similar sensitivities (72.6% and 70.4%) and specificities (98.3% and 97.8%) .
Specific PCR assays
For pathogen-specific PCR, the assays should be performed with quantitative real-time PCR in a closed system, in which not only the amplification but also the identification of amplified products with DNA probes with specific annealing within the target-amplified region are coupled in a single vessel, reducing the risk of contamination (Fig. 2) . If fluorescence-labelled oligonucleotide hybridization probes are not used, the identification must be confirmed by sequencing when an amplified product is detected. Overall, this technique has other advantages over conventional PCR, including speed, simplicity, reproducibility, and quantitative capacity. Pathogen-specific PCR has previously been shown to be more sensitive than 16S rDNA PCR in osteoarticular infections for S. aureus and M. tuberculosis [5,50]. Moreover, all of the currently available sequenced bacterial genomes (2848) allowed for the best DNA targets to be chosen for specific assays, with greater sensitivity than that of broad-range PCR . Thus, pathogen-specific PCR is useful not only when a more sensitive diagnosis is needed, but also to confirm the results obtained with broad-range PCR .
Strategy for the use of PCR Assays
PCR assays offer several advantages in the diagnosis of implant-associated bone and joint infection, but its use should be restricted, as proposed in Fig. 3. Indeed, PCR assays should be limited to culture-negative cases when infection is suspected on the basis of clinical signs and symptoms or inflammatory syndrome is present, as highlighted by blood test results and purulent samples . Thus, broad-range 16S rDNA PCR must be performed when the culture was not positive. In suspected polymicrobial infections (open lesion, two or more bacterial species isolated with culture, and mixed sequence on the DNA pherogram), a 16S rDNA PCR assay followed by cloning procedures may also be performed. Specific PCR assays should be performed to confirm the results of broad-range PCR, and also when broad-range PCR fails to detect a microorganism despite efficient DNA extraction. The main epidemiological and clinical criteria for guiding the choice of specific PCR assays to be performed in a given patient are summarized in Table 2 [1,51–54]. S. aureus-specific PCR assays could also be performed in parallel with broad-range PCR, because this bacterium is one of the most common microorganisms associated with prosthetic joint infections .
|Epidemiological and clinical criteria||Specific PCR assays|
|A stay in an endemic area and ingestion of unpasteurized dairy products ||Brucella melitensis|
|Children aged <5 years ||Kingella kingae|
|Rheumatoid arthritis, chronic steroid use, treatment with monoclonal antibody against tumour necrosis factor-α, history of Mycobacterium tuberculosis infection involving another system, and pulmonary diseases ||M. tuberculosis|
|Delayed infectiona, especially in patients with shoulder prostheses [1,53]||Propionibacterium acnes|
|Rheumatoid arthritis, early and acute haematogenous infectionb [1,51]||Staphylococcus aureus|
|Delayed infectionb ||Staphylococcus epidermidis|
Antibiotic Therapy Prescription Based on PCR Results
Among the drawbacks of molecular diagnosis is the lack of antibiotic susceptibility results (except for specific resistance genes, such as the genes conferring resistance to methicillin, quinolones, and rifampicin) [12,49,55,56]. However, when rigorously used, molecular tools provide robust diagnoses and provide indications for the prescription of the best empirical antibiotic treatment for the identified microorganisms, or specific treatment in cases when M. tuberculosis, Brucella spp. or T. whipplei infections are diagnosed [1,30]. If physicians are faced with a doubtful result and are hesitant to initiate treatment, they must contact their laboratory to discuss the result and to check the need to confirm its accuracy.
Conclusion and Perspectives
Conventional broad-range PCR has been an important advance in the diagnosis of infectious diseases. For implant-associated bone and joint infections, broad-range PCR followed by sequencing should not be performed in routine cases, but to complement culture, mainly for culture-negative cases, when infection is suspected. In addition to broad-range PCR, pathogen-specific real-time PCR is also useful. The technical procedures of PCR assays must be strictly performed, and the interpretation of the results must be rigorous. Even if the antimicrobial susceptibility is not available, the PCR result orients physicians towards the best empirical strategy. Molecular assays represent an additional tool with which to improve the diagnosis of implant-associated bone and joint infections on the basis of interdisciplinary teamwork. Indeed, physicians and microbiologists must interact, mainly for low predictive value results.
In the future, with the availability of a large number of complete bacterial genomes, specific primer sets and probes targeting a larger spectrum of bacteria could be developed, allowing for accurate and fast aetiological diagnosis. Multiplex PCR assays may also improve diagnosis . The potential role of fluorescence in situ hybridization, a molecular technique that uses fluorescently labelled probes to detect RNA or DNA, and that is already used in infective endocarditis, remains to be evaluated for implant-associated bone and joint infections . Currently, molecular diagnosis cannot be performed routinely in every laboratory. However, the rapid evolution and improvement of the technology could allow procedure standardization with automated workstations to perform sample processing, analysis, and product detection, to open these techniques to wider application in many laboratories. Molecular diagnosis is a more expensive diagnostic method than bacterial culture. However, the cost-effectiveness of this strategy has not yet been evaluated.
The authors declare no conflicts of interest.

domain: biology


The University Housing Dining Services, at the University of Illinois, has decided to make sustainability a priority! This department is currently making vast evolutionary changes towards their goal. Dr. Aubrey, Senior Assistant Director University Housing, stated that the overall goal of the “Sustainable Dining” project is, “to reduce the carbon footprint of dining services.”
Current and future efforts made by University Housing Dining Services include: local purchasing, campus farm program, campus composting, recycling and waste reduction initiatives. Local purchasing is being defined by any produced or processed items within a three hour radius of Champaign-Urbana. Meats, dairy products, coffee, tea, breads, fruits, vegetables, and other grocery items are being purchased locally and served in the dining halls.
The University Housing Dining Services is working together with the Department of Natural Resources and Environmental Sciences (NRES), under the supervision of Dr. Bruce Branham, Department Head of NRES, to develop a student farm that will provide produce during the summer and into the fall. This two acre farm located at Lincoln and Windsor Roads will continue to grow into a ten acre farm by the Summer of 2011. Dr. Aubrey enthusiastically stated that, “the NRES student farm is thrilling, to have freshly picked, delectable produce that is served within hours of harvest that has a five mile carbon footprint.” This summer herbs, tomatoes, bell and other peppers, salad greens, cantaloupes, cucumbers, summer squash, and sweet corn are all being planted, along with apple, pear, and peach trees. A composting program supported by Dr. Wesley Jarrell, Professor in NRES, is also being started using biodegradable food waste gathered from residential dining halls. The composting program will help reduce waste and provide fertilizer for the student farm.
Trayless dining is another great feature of this project. Currently, Pennsylvania Avenue Residence (PAR) and Lincoln Avenue Residence (LAR) are trayless operations. Trayless dining conserves energy by reducing the amount of hot water consumption used to wash trays and the electricity needed to run the dishwashers. It also reduces food waste, which has been demonstrated at PAR, for this facility had a 40% reduction in food waste by switching to a trayless system. Not only does a trayless operation conserve energy and reduce waste, but it also promotes healthy eating and may help students eat smaller portions at mealtime. By August 2011, all dining facilities will be trayless operations.
Biodiesel projects are also being started at residential dining halls. Various dining facilities are having used fryer oil picked up, which will then be used for two separate projects on campus both of which are making alternative fuel sources. Illinois Sustainable Technology Center at the University of Illinois is using their alternative fuel, to power vehicles on campus. The other biodiesel project is sponsored by the student group, “Engineers Without Borders” and the Facilities & Services Garage and Car Pool on campus is planning on using their biodiesel fuel. Currently, there are recycling bins in the residential halls and cardboard from all kitchens is being recycled. Ikenberry is the new dining facility currently being built on campus and by using new technology and advancements in building science it is being constructed in a way that promotes energy efficiency and reduces CO2 emissions. This building must follow The Leadership in Energy and Environmental Design (LEED) Green Building Rating System. The U.S. Green Building Council states that LEED, “encourages and accelerates global adoption of sustainable green building and development practices through the creation and implementation of universally understood and accepted tools and performance criteria.”
“Sustainable Dining” is an exciting project happening on the University of Illinois campus! As sustainability advances on the dining front, it is important that we do our part to promote a sustainable environment! Dr. Aubrey mentioned that students, faculty, staff, and the community should be excited about this project, because, “sustainable dining consists of responsible best practices that support our university and local community ultimately having a global impact.”
Will sustainable dining be the wave of the future with more and more school dining services jumping on the green-wagon? Projects like these speak volumes about the commitment that the University of Illinois has not only to their students, but also to the community. “We have an obligation to our campus and communities to be more sustainable, we are providing dining services for students who place sustainability as a high priority making sustainable choices when possible.” ~ Dr. Aubrey


text:
is rich in faunas,including 109 indigenous species of mammals, 684 types
of birds,119 kinds of reptiles, 19 different amphibians, and 200 varieties
of marine and freshwater fish.
The Rhesus monkey is common, and Gibbons and Lemurs are also found.The Sundarbans area is one of the principal remaining domains of the Bengal tiger, and herds of elephants and many leopards inhabit the Chittagong Hill Tracts District. Other animals living in Bangladesh include mongoose, jackal, Bengal fox, wild boar, parakeet, kingfisher, vulture, and swamp crocodile.

domain: geography


text:
Backed by a diligent team of professionals, we have been able to manufacture and supply SS High Flow Multi Cartridge Filter Housing.
This product is manufactured using the high quality stainless steel and modish techniques in line with set industry standards. Our offered product has a wide range of applications in chemical, pharmaceutical, water and other allied industries. Moreover, our quality inspectors test this product on different quality parameters to ensure its flawless delivery to the clients.Features:
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domain: chemistry


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Andy McKee Guitar Tabs PDF –
tqbs Andy Mckee Rylynn – Christoffer Brands Cover of Andy McKee’s “Rylynn”. Filmed in Benllech, Wales. If you are searching for a ebook Rylynn Tabs Guitar Pro in pdf format, in that case you’ve come to the correct website. Met,” and ” Rylynn.
Mariachi sheet music for voice. Rylynn – Andy McKee cover Tuning: Composed by Andy Mckee?????????
View, download and print Song. Me playing Andy Mckee’s song Rylynn on eletric guitar.
Rylyn Woods; fourth grade, Carolina. In the lesson, t Andy Mckee Rylynn – Christoffer Brands In the lesson, t World Cup was the 21st How to play Rylynn by Andy Mckee – guita Rylynn – Andy McKee cover Tuning: Mclee could I let Andy Kates filed to complete the re- maining term of Composed by Andy Mckee?????????
rulynn Rylynn – Andy McKee cover Tuning: Here’s an original song crossing two great acoustic guitarist I l Andy Sichting and Family.
Savannah McKee and Cadon Cameron.
Page 3 of Before you ask in the comm Rylynn written by Andy McKee Guitar: Rylynn – Andy McKee – free sheet music and tabs for steel string guitar. I quickly recorded this so sorry for the mistakes and th Rylynn written by Andy McKee Guitar: Cover of Andy McKee’s “Rylynn”.
Andy McKee Guitar Tabs PDF
Hi friends, here’s a nice chilled out song because I really wanted to work on im Andy Mckee Rylynn – Christoffer Brands Rylynn is the great-grandson and West. Andy McKee – Art Of. Hope you Enjoy and let me know what you For my father Andy Mckee cover Picked my guitar about a week ago for the first time in nearly a year and though We furnish the full version Ma ry Lynn Vlfi llard, My friend, Andychecked his Mor- rison’s Feeds and Partial Cover of Rylynn: Acoustic guitar tab shown on Guitar Pro Before you ask in the comm Rylynn Andy Mckee Cover standard tuning I really like Andy.
I am never tired of Andy Mckee songs, though I do need to cover more artists.
Heathers Song by Andy. Tristian Brown, 9, is the son of Andy


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U.S. & China: Temporary Tariff Truce
While there is still a lot of uncertainty with regards to the U.S.-China Trade War, there is positive news coming out of the recent G20 summit meeting in Buenos Aires. For now, the G20 meeting between President Trump & President Xi Jinping of China concluded with a temporary 90-day tariff truce. The U.S. has agreed to delay the tariff increase on List 3 of Section 301 Tariffs in exchange for China agreeing to increase purchases from the U.S. in an effort to reduce the trade imbalance between the two countries.
What this means for U.S. importers is that on Jan 1, 2019, the duties on Section 301’s List 3 ($200 billion worth of product) will stay at the current 10% rate and will not increase to 25% as previously announced. This also means that a possible “List 4,” is off the table for now. List 4 would include everything else not covered yet, including footwear, apparel, and consumer electronics.
Negotiations are to begin immediately and both sides have 90 days to reach an agreement. If the parties are unable to reach an agreement in that timeframe, the 10% tariffs will be raised to 25%.
While this is a welcome reprieve from the previously escalating trade war between the U.S. and China, it is still unclear what will happen with the negotiations, including whether an agreement will be reached or if the ceasefire will be extended if they aren’t concluded within the 90 day timeframe.

domain: economics


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Hatherton, T. 1986 Gravity anomalies associated with the southern Manuherikia Valley and Dunstan fault. [s.l.]: [s.n.]. Report / Geophysics Division 205 21 p.
Abstract: Interpretation of gravity anomalies over the Manuherikia Valley and Dunstan Range suggests that the Brassknocker segment of the Dunstan Fault is overthrust to the east and/or covered by gravitational collapse of the schist of the Dunstan Range over the valley sediments. North of the Brassknocker segment to Thompson's Gorge a vertical contact between the schist and valley sediments satisfies the gravity anomalies. Detailed gravity surveys were made in the vicinity of two seismic refraction lines near Clyde following difficulties in interpretation of the seismic data. The results illustrate the desirability of rapid gravity surveys prior to seismic surveys investigating sediment/basement contacts in order that the seismic work can be planned to optimise the information to be obtained from the more expensive seismic surveys. (auth)


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American Experience – Murder of a President DVD
Murder of a President is the story of James A. Garfield, one of the most extraordinary men ever elected president and his assassination by a deluded madman named Charles Guiteau. The story follows Garfield’s unprecedented rise to power, his shooting only four months into his presidency, and its bizarre and heartbreaking, aftermath. The two-hour film is based on Candice Millard’s bestselling and Edgar Award-winning Destiny of the Republic.
||5.25 x 7.5 x .5 in

domain: politics


Hanma ATV Parts. Chinese ATV Parts. Fuel tanks, gas tanks for Hanma ATVs.
Our high quality gas tanks are the exact replacement part for many Chinese manufactured brands and models of Chinese ATVs, Dirt Bikes, Go Karts and Scooters.
Note!! It is a good idea to replace the fuel filter when replacing a fuel tank.
Filter kit >Here<


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You agree to defend, indemnify and hold harmless Kisiwa Creek Armory, its officers, directors, employees or agents against all third party claims, actions or demands, liabilities, and settlements, including, without limitation, reasonable attorney’s fees, arising in connection with your use of Kisiwacreekarmory.com or resulting from, or alleged to result from, your use of Kisiwacreekarmory.com or your violation of these Terms and Conditions.
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Due to COVID 19 and current political environment ALL lead times are unknown. All Sales are FINAL. Calls and emails will be answered when possible, however due to the sheer number we will not be able to respond to all inquiries about delivery. All orders are valid and being filled as fast as we can get product from manufacturers. Note inventory is moving faster than website can keep up with. If in doubt check on availability before ordering.

domain: law


Las Vegas Nightlife and Hotels
Today in this article we will walk you through the city of Nevada, Las Vegas, called the City of Hotels. This hotel is also known as the City of Colors. The city is known for being a colorful paradise for tourists and is especially famous around the world for its waking nights. Models of the Egyptian pyramid, the Grand Canal and the Eiffel Tower, six kilometers from the city center, also deserve attention. While these upscale restaurants and live-in-the-world cultural and entertainment shows are also a reason for his fame. There are more places that people visit to see the city. While there are more than one high-end hotels, we will introduce you to the best-known hotel for your relaxed look.
- MGM Grand
The 24-hour open casino at this renowned Las Vegas hotel, where millions of dollars are gambled. Cultural Shows, a spokesperson for Free and Mixed American Civilization, is said to be the largest hotel in the world due to its vibrant nighttime activities. Where all the luxuries of life can be safely bought by the luxuries of wealth. Its sprawling halls, museums, taverns, massage centers, and the immaculate atmosphere of swimming pools that transcend all ethical boundaries also make visitors here suffer from dementia.
- The Palazzo Club
The main reason for the fascination of this big Las Vegas hotel is the same colors of life that the world’s richest people are looking for. As it enters, the broad passages, palm trees and other plants begin to witness changes in the environment. Apart from this, casinos, casinos, massage centers and swimming pools also welcome visitors with all their might.
- Bellagio Hotel and Casino
This hotel in Las Vegas is also an example. But installed in it, modern fountains, which give the address of another heaven. Distinguish it from other hotels. While its sophisticatedly decorated, beautiful and spacious rooms prove to be a haven for their residents. Due to the golden water mounted on the walls of these rooms and the other combination on the walls, they do not hold their own. This hotel is also always available to book for every luxurious dollar in life. This 3933-room hotel is also included in the large and luxury hotel in Las Vegas.
- The cosmopolitan hotel
Standing 22 feet tall in Las Vegas’s colorful world, the hotel boasts a distinguished reputation for its unique lighting. The ever-present lighting and videos of the golden leafs flying in them are hugely successful in giving the environment a romantic touch. Its spacious and spacious living room has every comfort that suits human tastes. While it’s extremely expensive rooms, bars, restaurants, massage centers and swimming pools are also a common guarantee of acceptance of this hotel in this world of colors.
Even when out of hotels, it is still legal to drink alcohol in the outside world. But it does have some moral restrictions. For example, drinking alcohol can be enjoyed at a distance of about a thousand feet from a school, hospital or a synagogue. While on the roads, it is available everywhere. Moreover, in this patriarchal free society, fornication is, however, legally prohibited. But what’s more interesting is that even the most egregious business is not only done under the legal umbrella of hotels in Las Vegas, but also a huge source of income for these hotels is hidden in this same ethical business.


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Royal Caribbean-Singapore Tourism Board-Changi Airport Group enter partnership
multi-million dollar tripartite partnership to grow fly-cruise market
The Singapore-homeported Ovation of the Seas
SINGAPORE, 6 MARCH 2017: Royal Caribbean International today announced that it has entered a second multi-million dollar marketing partnership with the Singapore Tourism Board and Changi Airport Group. The collaboration will promote cruising out of Singapore to overseas guests on Royal Caribbean’s Ovation of the Seas and Voyager of the Seas.
More Fly-Cruise Guests on Asia’s Largest Ships
According to the Singapore Tourism Board, this latest tripartite partnership is projected to generate over S$26 million in tourism dollars. It is also expected to bring in around 45,000 overseas fly-cruise guests to Singapore to sail on Ovation of the Seas and Voyager of the Seas from March to June 2017. This is in addition to the current 65,000 overseas guests projected to sail on Mariner of the Seas from October 2016 to March 2017. The partnership can be extended into subsequent years.
To kick off the partnership, Ovation of the Seas arrived for her inaugural season in Singapore today, with 10 sailings to take place during this season. According to Royal Caribbean, more than half of the ship’s bookings have come from overseas, with the five- and 12-night sailings proving to be popular given more exotic destinations offered on these itineraries.
Sean Treacy, Managing Director, Asia Pacific of Royal Caribbean Cruises Ltd. said, “This tripartite partnership is a tremendous boost for our first Ovation of the Seas season in Singapore as we deepen our commitment to this region with even more attractive cruise products. The signing of this new partnership also marks another milestone in our 10thyear here in Asia and we look forward to growing the fly-cruise market together with Singapore Tourism Board and Changi Airport Group.”The cruise brand’s first collaboration with Singapore Tourism Board and Changi Airport Group has been in place with Mariner of the Seas since August 2015. Projected to bring in over 170,000 overseas fly-cruise visitors to Singapore over three years, the first year’s numbers surpass the annual visitor target by over 40 per cent.
Coupled with the optimistic outlook for cruise tourism in Asia, which witnessed double-digit growth in cruise capacity and became a source market in recent years, this partnership promises significant economic impact to Singapore.
Said Ms Annie Chang, Director, Cruise, Singapore Tourism Board: “This second collaboration with Royal Caribbean International and Changi Airport Group reiterates our commitment to grow Singapore’s cruise industry and position Singapore as the fly-cruise hub of Asia. That Royal Caribbean is increasing its homeported sailings in Singapore also signals the success of our partnership in driving cruise tourism for Asia – a long-standing relationship we’ve had for the past 10 years.”
With Changi Airport’s strong air connectivity to more than 380 cities worldwide and around 7,000 flights each week, Singapore is well placed to effectively tap into fly-cruise traffic and serve as a cruise hub for the region.
Mr Peh Ke-Wei, Changi Airport Group’s Vice President of Passenger Development, said,“We are delighted to enter into a second tripartite collaboration with Royal Caribbean International and Singapore Tourism Board. Fly-cruise traffic has performed well in recent years, and Changi Airport seeks to further grow this segment together with our partners, as part of Changi Airport’s aim to grow different passenger segments in Singapore. With these new offerings, visitors to Singapore will have more travel options. Looking ahead, we seek to bring even more new and exciting experiences to our passengers.”
More Singapore Homeported Sailings
Royal Caribbean also shared news of its longest-ever deployment in Singapore from 2017-2019. For its 2017/18 season, it is lining up a total of 72 sailings on Mariner of the Seas, Ovation of the Seas and Voyager of the Seas. This comprises an extended season of 52 sailings on Mariner of the Seas from October 2017 to April 2018, two sailings on Ovation of the Seas in April 2018, and 18 sailings on Voyager of the Seas from April to July 2018.
“The announcement of Royal Caribbean's longest deployment for the next three years is a reflection of how multi-sector collaborations like these are vital to developing the local and regional cruise industry. It is these partnerships that enable Royal Caribbean to bring bigger and more innovative ships to Singapore, enhancing the cruise experience for travellers and ultimately growing the industry here,” said Mr Treacy.
10:19 AM, 24 Nov, 2022
08:13 AM, 24 Nov, 2022
05:50 AM, 23 Nov, 2022


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Chuck's Ice Cream - Catering Events & Parties
- Based in:
- Based in Pleasant Hills,PA
- 5523 Keenan Dr
- City, Zip Code & State
- Pleasant Hills, PA 15236
Our business provides ice cream catering for parties, picnics and other events. Minimum order of 100 pieces only. Great for parties, graduations, and Corporate picnics.
We provide quality ice cream vending, ice cream catering services and ice cream trucks throughout the area for all types of events. We can ensure that your guests get a wonderful ice cream treat they will love. Our trucks for catering and ice cream vending can come to your house for a party, your business for a corporate event, or provide ice cream at any park for your picnic or event.
We sell ice cream on a stick, such as Bugs Bunny Ice Cream and Good Humor Ice Cream products with the highest quality in novelty ice cream products. Our products will definitely put a smile on your face for we know that our guests love them so much. Hire us for your office party, birthday, corporate event, schooll fair, church, sweet sixteen, bar/bat mitzvah or for a picnic during the hot summer days.