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Industrial design and loft style are the hot trends in interior design’s current state of affairs.
Industrial design, in its essence, is to treat your home as an industrial creation, putting emphasis on functionality and practicality. The aim of this design is to give the idea of home a new definition, that it is a space for work as much as relaxation.
Industrial design main features
The basic materials of Industrial design are metal, concrete and bricks. Decorative materials include wood, leather and fabric.
To heighten the industrial atmosphere, the furniture of choice is full of presence, substantial and sturdy. The palette is a mixture of silvers, dark browns and blacks.
Pipes and beams can be exposed, left in their most original form. This is arguably the most Industrial-like element of the design.
In America, abandoned warehouses, factories and commercial buildings exist in the form of wide, open areas. Therefore, designers often fill the space with furniture with a lot of presence, so as to transform the space into a living environment.
This way, harmony and a homely atmosphere can linger amidst the rough textures and lines.
But since houses and flats in Hong Kong involve a lot of wall partitioning, if you’re channeling an industrial feel in your abode, tearing down walls might be necessary.
Shades of black
Black shades are the characteristic trait of Industrial design. In fact, a lot of cafés and bookstores are decorated in an Industrial style.
To paint all the walls and floors black or grey might not be so suitable for the small apartments in Hong Kong; it might give the interior a claustrophobic feeling.
Nonetheless, black decoration and furniture can be great alternatives.
Industrial design cannot do without metallic materials. Although the metal of choice is usually steel, options are in fact unlimited.
Metal chairs and tables with metal legs are indispensable. Preferably, the furniture should be in a degree of uniformity. Tables with machinery components as decoration look tastefully industrial.
Most furniture items are made with at least a small amount of wood, which contrasts beautifully with metallic elements.
Besides, you can add extra metallic components with storage boxes, food containers and racks.
Arguably the centrepiece of the living room, the leather sofa is a comforting addition to the interior, which neutralises the coldness of metals.
The soft lustre of authentic leather gives the space warmth.
Besides, fabric products can also be used as neutralising ornaments. They include table cloth, cushions and carpets.
One unspoken trait of Industrial design is that it seems to be a thing of the past. Objects with colourful backstories are very much an essence of Industrial design. Second-hand furniture like scratched sofas can perfectly satisfy this stylistic criterion.
Some Industrial-style enthusiasts purchase flawed and weathered furniture from second-hand stores, which saves a large amount of renovation cost.
If you’re keen on hanging prints to decorate the walls, black-and-white photos are the better option over colourful pictures.
Old bricks and rough concrete walls leave an unaffected impression in the beholder. If you plan to bring them into your home, be prepared to spend extra money on renovation cost.
Alternatively, you can find realistically printed wallpaper. Since the technology for wallpaper making has improved a lot in recent years, you can easily find red brick wallpaper with very realistic textures.
Lighting in Industrial design tend to be simplistic. In a space full of presence, strong lighting is a must.
Lampshades can be stripped off to expose the light bulbs. The classic beauty of the incandescent light bulb, or the trending Pasadena lamp above, are both perfect lighting for the Industrial design.
Extra tips: metal lockers
Ever thought of fashioning the wardrobes and shoe organisers into metal lockers you can only find in schools and offices?
They can look very industrial, but are likely involve a lot of DIY efforts. Whether that’s worth the effort, well, it’s for you to decide!
All in all, Industrial designs aims at transforming a home into the environment of a warehouse or a factory. Vice versa, a warehouse into a living environment. A lot of emphasis is put on functionality and simplicity, so that the inherent beauty of each object can compose a contrasting, interesting work of art.


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On a winter night last year, a Harford County deputy sheriff found Boyce Atkinson asleep -- and apparently drunk -- inside his Jeep on the side of U.S. 40 in Edgewood. Mr. Atkinson was charged with driving under the influence and taken into custody. Last week, the Maryland Court of Appeals tossed out the conviction in that case. It ruled that, in Maryland at least, there is no law against being "parked while intoxicated."
The initial reaction from law enforcement circles suggested a victory for the drunken driver.
The ruling is nothing of the sort, however. In its verdict, which was interestingly by a 7-0 vote, the court opined: "We do not believe the legislature meant to forbid those intoxicated individuals who emerge from a tavern at closing time on a cold winter night from merely entering their vehicles to seek shelter while they sleep off the effects of alcohol," Judge Howard S. Chasanow wrote.
What helped convince the judges to discard the conviction was that Mr. Atkinson was parked not far from several drinking establishments. The judges felt it could be argued that Mr. Atkinson hadn't driven his vehicle at all and had merely walked to it and fallen asleep in it. Better that a drunk uses his car for shelter than for transportation, the judges seemed to be saying. Amen to that.
If someone makes the bad judgment of getting in a car and attempting to drive while drunk, he or she should be punished. But shouldn't he or she receive some leniency for having the good judgment to pull off the road before causing damage?
Actually, the court's ruling was pretty narrow: If you're driving drunk, see a police officer, pull over to the roadside and go to sleep, expect to be arrested. The court said that six conditions -- whether the engine is warm, the headlights are on, the keys are in the ignition, the position of the driver and vehicle, and whether the vehicle is legally parked -- will help determine if the driver is "in control of the vehicle."
The morning after the ruling, even before Mr. Atkinson had been informed of it, State Police were already toning down their initial, official disgruntlement. The ruling shouldn't affect DWI patrols too much: Out of every 1,000 drunken driving arrests, maybe three fall in this gray area, police said.
Plus, they acknowledge -- and we agree -- that law enforcement's goal shouldn't be to bar motorists from sleeping off their stupors safely on the shoulder of the road, but to discourage and prosecute drunken drivers.

domain: law


Buzzing about his next role! Ryan Gosling 'to play 1930s musical choreographer Busby Berkeley in new biopic'
Ryan Gosling is reportedly in line to play musical choreographer Busby Berkeley in a new biopic.
The 33-year-old actor is set to portray the Hollywood director in upcoming film Buzz: The Life and Art of Busby Berkeley, based on Jeffrey Spivak's book of the same name, and the star will produce the project alongside Marc Platt.
According to The Hollywood Reporter, Gosling may also direct the motion picture, which currently doesn't have a writer.
New role: Ryan Gosling is reportedly set to portray musical choreographer Busby Berkeley in upcoming biopic Buzz: The Life and Art of Busby Berkeley
Director: Berkeley - who died in 1976 aged 80 - was well known for choreographing extravagant dance routines in several sparkling musicals
Gosling – has been dating Eva Mendes since 2011 – will team up with Platt once again after the pair collaborated on 2011 crime film Drive.
Berkeley – who died in 1976 aged 80 - was well known for choreographing extravagant dance routines in several sparkling musicals.
One of the most memorable images of Berkeley's productions is taken from 1933 film Footlight Parade.
Spectacular: One of the most memorable overhead images of Berkeley's productions is taken from 1933 film Footlight Parade and features girls with their legs out resembling a kaleidoscope
Sensational: Some of Busby's most famous productions include 1933 musical Gold Diggers of 1933 (pictured) and 1935's Gold Diggers of 1935
Successful career: The star also choreographed and directed 1930 musical comedy movie Whoopee! (pictured) and 1934's Dames
The overhead shot features girls with their legs out and resembling a kaleidoscope while dancing to the song By A Waterfall on a stage specially constructed by Warner Bros. for the director's project.
Berkeley's choreography was hugely successful and even earned him three Academy Award nominations for Best Dance Direction, a category which has since been axed.
The filmmaker once said one of his main intentions was to just ensure people feel 'happy' when they watch his productions.
Recognition: Berkeley's choreography was hugely successful and even earned him three Academy Award nominations for Best Dance Direction, a category which has since been axed
He explained: 'In an era of breadlines, depression and wars.
'I tried to help people get away from all the misery ... to turn their minds to something else. I wanted to make people happy, if only for an hour.'
Some of the choreographer's most famous productions include 1933 musical Gold Diggers of 1933 and 1935's Gold Diggers of 1935, while he also choreographed and directed 1934 musical comedy movie Dames.
Gosling is currently working on an untitled musical drama which is being directed by Terrence Malick and stars Christian Bale, Michael Fassbender and Cate Blanchett.
He will hit the big screen in forthcoming film How to Catch a Monster, which will be released later this year.
In happier times: Busby married US actress Merna Kennedy in 1934 but they split just a year later
Happy couple: Ryan has been dating Eva Mendes since 2011, and the brunette beauty recently declared her admiration for him
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Personalised Gaming Controller LED Light
ONLY AT MENKIND It’s a night light inspired by a retro games controller! Personalise it with up to 12 characters of text. Light can change between 6 amazing colours. Powered by USB or 3 x AA batteries (not included). Measures approx. 17 cm x 9 cm x 9 cm.


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Russian Shoppers Swarm New IKEA Store in MoscowAired April 18, 2000 - 2:54 p.m. ET
THIS IS A RUSH TRANSCRIPT. THIS COPY MAY NOT BE IN ITS FINAL FORM AND MAY BE UPDATED.
NATALIE ALLEN, CNN ANCHOR: There was a near stampede at a Russian market today. Not another stock panic, it was furniture. The Swedish firm IKEA has set up shop in the land of Lenin.
Here's CNN's Steve Harrigan.
STEVE HARRIGAN, CNN CORRESPONDENT (voice-over): They lined up in the cold 40,000 strong, then set off at a run when the doors opened, all to buy furniture.
LENNART DAHLGREN, IKEA RUSSIA: We had about 50 percent more sales, so they bought everything.
HARRIGAN: They are the Russian middle class, a group many thought had disappeared after a financial crisis two years ago, young families like policeman Maxim Borisov and his wife Natasha, an accountant.
NATASHA BORISOV, ACCOUNTANT (through translator): It's my dream to have a modern Italian kitchen.
HARRIGAN: For the Borisovs, as for many Russians, space is tight. At night, the living room doubles as a bedroom.
MAXIM BORISOV, POLICE OFFICER (through translator): We're not going to die from hunger. We've got clothes on our backs, and sometimes enough to go to the theater. I've got no yacht, no dacha, but we live like human beings.
HARRIGAN: The size of the Russian middle class is not easy to judge, making furniture giant IKEA's $40 million store a risky investment.
DAHLGREN: If you read the statistics, it seems like you haven't anything to spend. But if you look at the way you spend, you have a lot to spend. So it doesn't fit together.
HARRIGAN: With a steady ruble and a new government in place, other retailers may follow. But the road to Moscow is a slow one. It took IKEA 12 years to open its first store. And the fight is not over yet. (on camera): Moscow region says the store has to build a bridge to reduce traffic. But Moscow city says the bridge would block the view of a World War II monument.
(voice-over): The region controls the land but the city controls the road. That leaves the bridge half built and many of the drivers around the store stuck.
Steve Harrigan, CNN, Khimki, Russia.
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Citizenships Are Now Up For Sale As NFTs On Marketplace
With the rise in non-fungible tokens (NFTs) popularity, the space has seen the sale of some interesting things and pieces on the blockchain. In Feb. 2022, an artist blew up a Lamborghini Huracan and sold the charred remains as NFTs. British artist Damien Hirst burned hundreds of physical artworks after selling them as NFTs on the blockchain. Now, citizenships are being sold and traded as NFTs on marketplaces.
Buy Your Way To Satoshi Island
An ambitious development project is planning to create a Satoshi Island that would turn a South Pacific island into a haven for crypto investors. This island is Lataro Island which is currently mostly a virgin rainforest that will be turned into a sustainable smart city. At the head of this project are property developers Anthony and Theresa Welsh, who currently inhabit the 300-hectare island.
They plan to turn the island into a community of crypto investors. The biggest draw of the island is the lack of any income taxes there. Anthony Welch explained that the plans are for 21,000 investors for the island and for anyone to buy or develop land, they would need to hold a citizenship NFT currently being traded on marketplaces.
Each NFT minted usually represents some type of property being sold. It also provides exclusive access to the island which will only have 21,000 citizens. However, for those who do not wish to become a citizen, the property developer says there are plans for a resort so crypto investors can enjoy the island without citizenship.
Citizenship Passes were minted on Oct. 30 and have almost 1,000 ETH volume already on OpenSea.
NFTs Are Not Dead
NFTs are a long way away from their glory days but that does not mean that they have been completely written off. Even during the crypto winter, they are still making waves as creators find innovative ways to apply NFT ownership to their projects.
NFT marketplaces see rise in volume | Source: DappRadar
These non-fungible tokens have been able to move away from in-game access to having some real-world utility such as the Citizenship NFTs. Trading volume has also been on the recovery side during this time as more traders return to the space.
OpenSea trading volume for the last 24 hours was up by more than 30% to $22.27 million. Trader numbers on the leading NFT marketplace also grew by over 42% during this time to sit at 34,267, according to data from DappRadar.
Featured image from MARCA, chart from TradingView.com
Follow Best Owie on Twitter for market insights, updates, and the occasional funny tweet…
Comments are closed.

domain: geography


text:
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This irresistible Gold and red Sherwani doesn't need a reason to steal the limelight. The advanced and extraordinary craftsmanship done on this suit is simply just sublime.
Milan is cut with precision and ornamented with fantastic floral artistry around the whole suit. The unique red shaped curves on the front end make the whole suit look and feel authentic. Golden piping around the collar and front slit accentuate the glamorous look of this Sherwani. The shoulders, sleeves, chest area and bottom of the Sherwani are super embellished with beads, crystals, and sequins inserted with dedication. There is also some elaborately detailed work done on the back so that the Sherwani looks great from all possible angles.
This Sherwani Set comes with a contrasting pair of Churidar Trousers.

domain: artistic


text:
04-13-2014, 07:02 PM
It's A Revolution
Join Date: Dec 2010
Location: Right Behind You
Spring Game Feature
Yo guys here's a link to some photo edits I'm posting from the Spring Game. Let me know what you think!
Attendance record vs. Clemson as a student: 5-0
Attendance record at home games as a student: 32-3

domain: sports


text:
Welcome to La Finca Royale
A large 3 bedroomed house with fantastic views of the bay of Cura beach, enjoy the sands of Amadores and the nearby golf course. La Finca Royale is a detached villa within its own complex of swimming pools, courts for tennis and squash and other sports, ideal for all the family.
Inside the Villa
2 double bedrooms, 1 bedroom has access to a separate terrace, the other bedroom is a twin bedroom.
2 Bathrooms one on the upper level and the second on the ground floor complete with wash basin, sink and shower unit.
The lounge sits comfortably with a dining area that accommodates up to 6 people. The kitchen is well equipped with microwave, dishwasher, washing machine, hob, oven, freezer, fridge, top oven and grill, toaster, coffee maker. The lounge has a sofa and 2 armchairs comfortably seats a large number of people with satellite, hi-fi, TV, DVD player.
There are is a main terrace and salarycalculator it is complete with its own small private pool overlooking the beach and the sea. Outdoor dining table and chairs are spread out around the villa, open space allows for a BBQ area or just sunbathing.
Altogether the villa sleeps 6 people in 3 bedrooms, there is an extra space for another person with the sofa being a pull out bed, all linen for the beds and towels for the bathrooms are provided.

domain: travel


Measurement of Amplifier Power
There are many factors that must be all taken together and the resulting answer after assessing the factors tells the VSAT owner what size of amplifier will be needed to run the satellite link efficiently.
Satellite amplifiers are not measured in physical size, they are measured in output power, most often referred to in watts. Even the manufacturers standard of measurement can be ambiguous, some of them measure the amplifiers output is PSAT, which means the power required to saturate the signal. A saturated signal is not usable, an amplifier measured in PSAT does not represent the practical maximum power output, a more accurate measurement is called P1dB and in this case if an amplifier is rated as say 100 watts, then all 100 watts can be used without distortion to the RF signal.
Although the measurement of power is important, it is just as important to know the frequency range of the amplifier. KU band frequency in the U.S. lies in the range of 14.00 GHz to 14.5 GHz, but many satellites in other regions have extended range of 13.75 to 14.5 GHz and therefor the amplifier used in those situations must be capable of transmitting at the extended frequency.
In broad terms the factors that must be considered when calculating the RF power of an amplifier are as follows:
- Size of the Earth Station Antenna
- Size of the VSAT Antenna
- The satellite carrier bandwidth
- Signal strength of the satellite at the VSAT location
- Rainfall at both ends of the satellite link
The actual calculation is performed by a formula called a Link Budget, in most cases the engineers in the Satellite Operation Center will perform the link budget calculation and advise how much power is needed in excellent weather, and how much extra may be needed to compensate for signal loss in case of bad weather.
In looking for additional reading, we found a PDF from iDirect that offers a technical and comprehensive discussion of the field of satellite communication.


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Enjoy hours of family fun! Familiar card games you know and love with a Latter,day Saint twist!
Ward FishSpend some time fishing for ward members! This fun card game promises hours of entertainment as you try to collect all the members of the ward, from the Ward Organist to the Nursery Leader to the Bishop. Fun for 2 to 6 players ages 5 and up.

domain: game


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Interview with: Una Petrauska, Managing Partner
Managing Partner of KPMG Law in Latvia Una Petrauska explains how KPMG Law in Latvia has grown as well as provides insight into how the firm is adapting to the changing business environment.
What do you see as the main points that differentiate KPMG Law in Latvia from your competitors?
In addition to KPMG Law being a classic full-service law firm, we are also a part of BIG4 law firms. We are considered alternative legal services provider, often associated with NewLaw concept. We differentiate with combined services (e.g. Tax, Deal advisory, IPO, Digitalization) and geographical coverage (more than 80 jurisdictions). Our competent and experienced team provides legal solutions integrated with other expert services of KPMG. In cooperation with KPMG global network, we are used to work side by side with lawyers from various jurisdictions providing legal services not only in M&A, classic regulatory and compliance areas but also offering legal assistance in cybersecurity, digitalization, ESG, data privacy, financial and fintech sector, dispute resolution, and other matters involving transformation and changes. KPMG Law is experienced in dealing with complex, cross-jurisdictional business and dispute issues, so we know the value of acting quickly and decisively.
Which practices do you see growing in the next 12 months? What are the drivers behind that?
The financial sector (covering IPO and Fintech) and compliance area will experience significant growth in the coming year due to the expending regulatory requirements and developments towards digitalization as businesses are changing rapidly. Assessments and complex support will be needed for businesses to develop. KPMG strives to be amongst most modern law firms and the drivers for that are process efficiency, digitalization, new approaches and requirements. Despite mentioned, we are aware and prepared that turbulent times may shake up businesses therefore we are keeping the pulse and polishing our knowledge in investment protection, dispute resolution, legal restructuring matters.
What’s the main change you’ve made in the firm that will benefit clients?
We are on our digitalization journey, looking for and implementing solutions to make our services competitive and more easily accessible for our clients in this changing and digitally driven environment. We are growing rapidly by involving industry experts in dispute resolution, IPO, FinTech, Intellectual Property Rights offering even deeper expertise, greater volume and speed. In the last year we have significantly increased our team by 40%. The DNS of our team members is to look at any topic from various angles and to seek solutions based on multi-service knowledge.
Is technology changing the way you interact with your clients, and the services you can provide them?
As previously stated we are currently on our digitalization journey. Our goal is to be a paperless office and we are very proud that we have already decreased our paper consumption by more than 90%. The implementation of technology is essential, it makes our services more accurate and effective. Of course, digital solutions also raise data issues, so we approach this matter with caution. The processing of the Latvian language is also a challenge in the implementation of digital solutions. However, we have successfully launched Whistle Blower tool as well as digital training on data privacy that clients can use at their convenience. It is also worth mentioning that our digitalization efforts make sense given that e-case was introduced in Latvian courts (all litigation documents are submitted and reviewed online).
Can you give us a practical example of how you have helped a client to add value to their business?
This is best described by our clients. As our clients have said – “KPMG team consists of experienced legal professionals with business-oriented mindset and ability to understand client needs. The team is ready to innovate and adjust the service offering to new types of business models which for us as client was a valuable experience.”
We strive at our day-to-day tasks to justify our client’s expectations therefore we devote a great deal of time in building team that consists of experienced legal professionals with business-oriented mindset and ability to understand client needs. The proof of this is our long-standing relationship with many of our clients and the acquisition of new clients thanks to word-of-mouth feedback.
As one of examples is management buy-out of APE Motors. KPMG Law together with KPMG Advisory team supported APE Motors in the acquisition of shares from the majority shareholder (Management Buy-Out – MBO), and the acquisition of a subsidiary in Estonia. Not only did KPMG Latvia structure of the deal and coordinate the deal value and the offer between the parties but also both attracted funds and coordinated the process of attracting funds. KPMG Law contributed to the deal by providing a full range of legal services and establishing a holding company in the form of a special purpose vehicle (SPV).
We can be part of the most complex multitasking projects as we work in synergy with KPMG Advisory, financial and tax experts covering all areas.
Are clients looking for stability and strategic direction from their law firms – where do you see the firm in three years’ time?
We look forward to provide complex and solution based advice which adds value to the clients’ businesses and support their growth. Services involving technical knowledge beside the legal expertise will grow due to the transformations and digitalization services. Automated tools will be involved to ensure routine legal assistance, and expert team will assist the clients with sophisticated solution advice.
We apply our values throughout our work. For example, integrity – we do what is right – and excellence – we never stop learning and improving. Our goal is to establish long-term relationships with our clients. We are successful at that and one example is Klasmann-Deilmann global leading growing media group – a company that came to Latvia in 2012, and since then we have been their legal advisor. We are developing as a trusted advisor.
We are constantly improving our knowledge as required by the market. Our goal is to stabilize our level of expertise on the one hand, and to provide input for legal growth on the other. The legal field is dynamic and combined knowledge is essential. We focus our knowledge on solutions; therefore, we see ourselves as a firm that offers added value. We have niche knowledge to find the most accurate solution for every need such as telemedicine, PPP and other areas.


Calculate Age as Whole Number using Power Automate
Dynamics 365 CE has been around for over a decade and in that time, Microsoft have not included a way to calculate age by default. I’ve never quite understood this as many companies like to wish people happy birthday and collect date of birth as information. There are several ways to calculate age but none of them ever seemed like a great solution to me.
You could use Business Rules because they include a datediff and UTCnow functions but, this rounds up to the month, so even if it’s not the person's birthday, it rounds it up.
I’ve looked into using workflows, I’ve never tried to have a waiting workflow for 365 days, but I doubt it would work, plus if you have tens of thousands of records or more, it would just not be sustainable.
Power Automate is a great tool, with the ability to do lots more than Classic CDS/D365 Workflows. One of it’s advantages is a proper scheduling engine, something we can take advantage of and run this Flow daily. This solves one problem.
Next, we want to be able to list records where it’s the person's birthday today. The CDS connector has a List Records option and you can query the ones you want to return; so this will solve another problem, which is that we don’t want to return tens of thousands of records each time we run this, instead we just get the 2 or 3 we need to update. However, this does actually lead us onto a secondary problem, how do we return records that have a matching day and month, but not year using odata? The answer, we don’t.
The mini solution
We need a way to return records where just the day and the month match in the Date of Birth field, to today's date, but when you work with date fields, it’s not something you can really specify. You can specify a date range, a specific date but not where just day and month match. Instead, what we will do is convert the date of birth into a string field and then take away the year.
But why? Well, with string fields, you can query records which “Equal” that string, so when we query the records, we can return just the ones where the birthday is today. If we tried to compare the birth date to today's date, it would not match because the year would be incorrect. Create a field which is of type string in your CDS instance and then we are going to create a Power Automate Flow that runs on Create of the record or update of the Date of Birth Field.
It’s important to note the create or update action is only available in the CDS Current Environment connector for Power Automate which can only be accessed inside a solution. It should looks something like this.
Next, create a compose action and in this step, we are going to add an expression which will convert the date of birth into a string and remove the year part.
The expression we want to use for this is:
The above formula converts the Date and Time of the date of birth field from UTC to just be day and month, then the “String” function converts that output to a string. Then we just update that record with another step.
And that’s part 1 of the solution, now onto the fun bit.
The Main Solution
We need to create a new Power Automate Flow which is a recurrence Flow which runs once a day.
Then we have a step which gets today's date and formats it into just the day and month. We can put this into a compose action and create an expression.
The above formula just formats the UTC now time and date. If you work in a drastically different timezone, you may need to do some adjusting at this point.
Next we want to query our database to return all records where the Date of Birth String field we populated in the first Flow, meet today's day and month.
The query is simple, where date of birth string = today's formatted date. That will return just the records where it’s the persons birthday today. Perfect.
Next we want to update these records, because it could be multiple records, we need an Apply to Each control around the next few steps. By adding an update record or using any dynamics content in a compose action, Power Automate will automatically put an Apply to Each control, but you can also add this in manually.
The first part in the Apply to Each control is a Parse JSON action because we need access to the data from the records listed, but when you used the Apply to Each Control, you lose access to this. By using the Parse JSON Action, we can interpret and use the data from the list records action and then use it. I’d recommend running the first bit of the Flow first, getting the JSON from the List records action and then using the sample payload button to generate the schema. I have a video on how to use the Parse JSON action here.
Now comes the magic, we have quite a long expression to get the required output. We create a Compose Action and then use the following expression.
There’s a lot of things happening in this expression so let's break this down so that you understand how we arrived at this.
This part of the expression subtracts the number of ticks of the time now, from the date of birth. A tick is defined as 100 nanoseconds from Jan 1st 1601 at UT 00:00:00, so by subtracting the two, we can get the difference between the current date and the birthdate in ticks.
The div (Divide) is dividing the number of ticks by 864000000000 (the number of ticks in a day) and then that answer by 365.25 (which is the number of days in a year roughly) and this gives you the number of years old the person is as a decimal.
Next part converts the decimal to a string, splits the string using the ‘.’ part of the decimal and then returns just the first answer.
The last bit converts the number to an integer, and add 1 to the answer. The reason for this is that when the answer comes back, we’ll be running this on the birthday, but until the day is over, it will still be a decimal of the previous age and not matching or over.
It may look all big and scary but it’s much simpler when you break it down. Then the last thing is to update the record. We choose the Contact dynamic content from the List Records action (it will actually be the apply to each but Power Automate is smart enough to figure that out), which is the item ID and put the output of this action into the age field.
And there we have it, a solution which returns a whole number, runs on a reliable scheduling engine, returns as an integer and...no code required. I’m really happy about this solution and I hope it helps others.
Ciao for now.


WASHINGTON — Stephen Bannon left his post as chief White House strategist Friday to return to the right-wing online Breitbart News, where he said he’ll be “going to war” from the outside for President Donald Trump.“If there’s any confusion out there, let me clear it up: I’m leaving the White House and going to war for Trump against his opponents — on Capitol Hill, in the media, and in corporate America,” Bannon said Friday in an interview with Bloomberg News hours after his departure was announced.
Bannon, an economic nationalist and populist who helped Trump win the election but skirmished with other White House advisers he criticized as “moderates,” exited with a vow to fight for his ideology.
“The Trump presidency that we fought for, and won, is over,” Bannon told the conservative Weekly Standard. “We still have a huge movement, and we will make something of this Trump presidency.”
He said Trump will find it harder to accomplish his larger goals because “there’s about to be a jailbreak of these moderate guys on the Hill” and aides will try to “moderate him.”
Bannon is the fifth senior official to depart from the White House in the past five weeks, exiting as Trump’s new chief of staff John Kelly revamps the system in an effort to end the turmoil at the upper levels of the administration.
Bannon has been a polarizing figure within the White House, pushing Trump to a more extreme position on trade and foreign policy, allegedly leaking dirt about his White House rivals, and warring with what he called the “Republican establishment.”
Bannon also clashed with other advisers, including Jared Kushner, Trump’s son-in-law, and Cabinet members from Wall Street.
His departure was cheered on the floor of the New York Stock Exchange and came after many top corporate CEOs quit Trump advisory panels because Trump’s remarks equivocated on denouncing white supremacists and blamed “both sides” for the racial violence in Virginia last Saturday.
Bannon said he had tendered his resignation on Aug. 7 effective a week later because “I’d always planned on spending one year.” But the tumult in Virginia delayed it, he said.
Bannon rejoined Breitbart News as executive chairman Friday afternoon and chaired the company’s evening editorial meeting, the online news outlet said.
“The populist-nationalist movement got a lot stronger today,” said Breitbart News editor-in-chief Alex Marlow.
White House press secretary Sarah Huckabee Sanders said on Friday, “White House Chief of Staff John Kelly and Steve Bannon have mutually agreed today would be Steve’s last day.”
Kelly has been reviewing the White House staff and may target other advisers for dismissal, according to news reports.
Following the street fights in Charlottesville between participants in a “pro-white rally” bearing Nazi and fascist symbols and counterprotesters, critics called for the ouster of Bannon and other “nationalists” in the White House.
At Trump Tower on Tuesday, Trump fended off questions raised by Sen. John McCain (R-Ariz.) about Bannon’s “alt-right” allies attacking National Security Director H.R. McMaster.
Asked about Bannon and his future, Trump said “he’s not a racist” and he’s a “friend of mine.” But Trump added, “We’ll see what happens with Mr. Bannon.”
The next day, Bannon contradicted administration policy on North Korea and its nuclear weapons — saying “there’s no military solution” despite Trump’s warning of “fire and fury” — in a phone call to a writer at The American Prospect, a liberal magazine. Bannon said he thought he was off the record.
Bannon called white nationalists “a collection of clowns” but also urged Democrats to talk about “racism every day” so Republicans could “crush them” in elections.
Rep. Peter King (R-Seaford) said the interview led him to urge Trump Thursday to fire Bannon for “undermining the president on foreign policy and encouraging in effect a political race war.”
Bannon had led Breitbart News since 2012 until he was brought into the Trump general election campaign — and the White House — by Robert Mercer, the billionaire co-president of the Renaissance Technologies hedge fund in East Setauket.
Now Bannon is back at Breitbart News.
“I feel jacked up,” he told the Weekly Standard. “And know I’m about to go back, knowing what I know, and we’re about to rev that machine up.”
- Jan. 30. Trump fires Sally Yates, the acting attorney general.
- Feb. 13. Michael Flynn resigns after 23 days on the job.
- March 10. Attorney General Jeff Sessions abruptly asks 46 U.S. attorneys to tender their resignations, not in itself an unusual move. Preet Bharara, U.S. Attorney for New York’s Southern District, refuses to comply and is fired.
- March 30. Katie Walsh, who came to the White House with chief of staff Reince Priebus, was sent out of the administration to ostensibly work for a pro-Trump political committee.
- May 9. FBI director James Comey is fired by Trump.
- May 30. White House communications director Mike Dubke resigns.
- July 6. The director of the Office of Government Ethics, Walter Shaub, resigns.
- July 20. Mark Corallo, spokesman for the legal team defending Trump on the Russia investigation, resigns. Lead attorney Marc Kasowitz steps back from that position.
- July 21. Press secretary Sean Spicer resigns after Anthony Scaramucci is hired to replace Dubke.
- July 25. Assistant press secretary Michael Short resigns after Scaramucci suggests he’s about to be fired.
- July 28. Chief of staff Reince Priebus is replaced with John Kelly, who runs the Department of Homeland Security.
- July 31. Communications director Anthony Scaramucci is removed from his position.
- August 18. Chief strategist Steve Bannon leaves his position.
— The Washington Post


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One of major objectives of crop breeding is conferring resistance to diseases and pests. However, large-scale phenotypic evaluation for many diseases and pests is difficult because strict controls are required to prevent their spread. Detection of disease resistance genes by using DNA markers may be an alternative approach to select potentially resistant accessions. Potato (Solanum tuberosum L.) breeders in Japan extensively use resistance gene H1, which confers nearly absolute resistance to potato cyst nematode (Globodera rostochiensis) pathotype Ro1, the only pathotype found in Japan. However, considering the possibility of accidental introduction of the other pathotypes, breeding of resistant varieties is an important strategy to prevent infestation by non-invading pathotypes in Japan. In this study, to evaluate the prevalence of resistance genes in Japanese genetic resources, we developed a multiplex PCR method that simultaneously detects 3 resistance genes, H1, Gpa2 and Gro1-4. We revealed that many Japanese varieties possess not only H1 but Gpa2, which are potentially resistant to other pathotypes of potato cyst nematode. On the other hand, no genotype was found to have the Gro1-4, indicating importance of introduction of varieties having Gro1-4. Our results demonstrate the applicability of DNA-marker assisted evaluation of resistant potato genotypes without phenotypic evaluation.
Conferring resistance to diseases and pests is a major goal of crop breeding. Traditionally, resistance is assessed by inoculating test plants with a disease or pest or by planting them in an infested field. However, the handling of many diseases and pests, which are not found or exist only in restricted areas in a country, is strictly restricted by quarantine. Therefore, large-scale phenotypic evaluation is impractical for breeding novel resistant varieties.
The use of DNA markers for detecting disease resistance genes may be an alternative approach to phenotypic evaluation (Collard et al. 2005, Jena and Mackill 2008). DNA markers, which are closely linked to disease resistance genes, enable the evaluation of resistance genes without inoculation. Such evaluation provides information about the prevalence of disease resistance genes in genetic resources and facilitates breeding of novel resistant varieties.
Potato cyst nematode (PCN) comprises 2 species (Globodera rostochiensis and G. pallida) and 8 pathotypes (Ro1 to Ro5 of G. rostochiensis and Pa1 to Pa3 of G. pallida). Infested potato plants show retarded growth and decreased yield compared with healthy plants. PCN causes yield losses ranging from 20% to 70% and up to 30% worldwide (Oerke et al. 1994). Thus these species are one of the most important pests of potato (Solanum tuberosum L.) and are therefore included in the list of quarantine pathogens in many countries. It can be controlled by crop rotation, chemical soil disinfestation and cultivation of resistant varieties. However, because of the formation of cysts, PCN can survive in soil for many years in the absence of host plants, making crop rotation unattractive for potato farmers. Chemical control of PCN involves unspecific and harmful pesticides. Because of increasing concern about environmental issues and governmental regulations, this method has been almost abandoned in many countries. Therefore, breeding and cultivation of resistant varieties are becoming increasingly important, and hence, scientific studies of the resistance genes and their underlying mechanisms are of great interest.
Several genes and quantitative trait loci (QTLs) of PCN resistance derived from other cultivated species and wild species have already been identified in potato. Thirteen resistance loci confer partial resistance: Gro1.2, Gro1.3, Gro1.4, Gpa, Grp1, Gpa5, Gpa6, GpaM1, GpaM2, GpaM3, RGp5-vrn HC, GpaXIltar and GpaIVSadg (Caromel et al. 2003, Kreike et al. 1993, 1994, 1996, Moloney et al. 2010, Sattarzadeh et al. 2006, Tan et al. 2009, van der Voort et al. 1998, 2000). Further, 4 loci (Gro1, H1, GroVI and Gpa2) (Barone et al. 1990, Gebhardt et al. 1993, Jacobs et al. 1996, van der Voort et al. 1997) and the combination of GpaVsspl and GpaXIsspl (Caromel et al. 2005) confer nearly absolute resistance to one or more pathotypes. Gro1.2, Gro1.3, Gro1.4, Gpa, GpaM1, GpaM2, GpaM3 and Gro1 were derived from S. spegazzinii Bitt.; RGp5-vrn HC and GroVI were derived from S. vernei Bitt. et. Wittm.; GpaXIltar was derived from S. tarijense Hawkes; GpaIVSadg, H1 and Gpa2 were derived from S. tuberosum ssp. andigena Hawkes; GpaVsspl and GpaXIsspl were derived from S. sparsipilum (Bitt.) Juz. et. Buk.; Grp1, Gpa5 and Gpa6 were derived from interspecific hybrid clones of S. tuberosum and several wild species including S. oplocense Hawkes, S. spegazzinii, S. tuberosum ssp. andigena, S. vernei and S. vernei ssp. ballsii.
Breeders have succeeded in producing potato varieties resistant to G. rostochiensis by introducing H1. H1 confers hypersensitive resistance to G. rostochiensis pathotypes Ro1 and Ro4 (Bakker et al. 2004, Gebhardt et al. 1993). However, widespread and extensive use of varieties with H1 has inadvertently caused rapid worldwide increase in G. pallida (Bradshaw et al. 1998, Bryan et al. 2002). Therefore, great emphasis is now placed on breeding of varieties resistant to a broad spectrum of PCN populations.
Recently, a set of DNA markers closely linked to H1, N146 and N195, was developed; these markers sandwich H1 with recombination frequencies of 0.109% and 0.207%, respectively and have been used for selecting PCN-resistant lines (Mori et al. 2011, Takeuchi et al. 2008). DNA marker Gro1-4 can detect Gro1-4 (Biryukova et al. 2008, Gebhardt et al. 2006). Gro1-4 is a member of the Gro1 locus, which confers nearly absolute resistance to all pathotypes of G. rostochiensis, and is therefore considered a useful resistance gene (Barone et al. 1990, Paal et al. 2004). Gpa2 provides dominant resistance to distinct G. pallida populations (Bakker et al. 2003, van der Voort et al. 1997, 1999); because this resistance gene has already been isolated and well characterized, markers directly amplifying the gene can be developed on the basis of its sequence information. Broad-spectrum resistance to G. rostochiensis and G. pallida is conferred by the Grp1 locus and detected by cleaved amplified polymorphic sequence (CAPS) marker TG432 (Finkers-Tomczak et al. 2009). CAPS marker C237 is linked to GpaIVSadg, which provides partial resistance to G. pallida Pa2/3 (Moloney et al. 2010). Single-nucleotide polymorphism (SNP) marker HC can detect RGp5-vrnHC, which confers partial resistance to G. pallida Pa2/3 (Sattarzadeh et al. 2006).
In Japan, breeding of PCN-resistant varieties was started just after pathotype Ro1 was discovered in 1972, and since then, achieving Ro1 resistance in new potato varieties has become the priority. Among the 8 pathotypes of PCN, only Ro1 has been found so far (Inagaki 1984, Kushida and Momota 2005). Because H1 provides perfect Ro1 resistance and has not been overcome by G. rostochiensis populations (Kushida and Momota 2005), breeders exclusively use this gene for breeding of Ro1-resistant varieties (Mori et al. 2007). However, despite the strict quarantine procedures in Japan, the possibility of accidental introduction of the other PCN pathotypes cannot be completely excluded. Therefore, selection of potato genotypes potentially resistant to several pathotypes and breeding of resistant varieties are an important strategy to prevent infestation by other pathotypes.
In this study, we tested published DNA markers and newly developed markers. Then, to identify candidate PCN-resistant accessions, we evaluated our genetic resources by a novel multiplex PCR method developed using selected DNA markers. Our results revealed the current status of resistance genes in our resources. Further, we discuss the possibility of evaluation of genetic resources for potential resistance to diseases and pests before actual invasion by using DNA markers.
Our genetic resources contain 812 varieties and lines including wild relatives, other cultivated species, landraces, modern varieties, and breeding lines derived from crosses among our resources and cover almost all varieties used in Japanese potato breeding. A list of the varieties and lines used in this study is shown in Supplementary Table 1. Total DNA was isolated by the hexadecyltrimethylammonium bromide method (Doyle and Doyle 1987).
To develop DNA markers and to determine the PCR conditions, samples were chosen on the basis of previous researches, evaluation data published in The European Cultivated Potato Database (http://www.europotato.org/menu.php), pedigree records and breeder’s evaluation.
Kita-akari, Touya and Haruka, which are known to have H1, were used to determine the PCR condition for N146 and N195. For developing Gpa2 markers, Marijke which has Gpa2 (van der Voort et al. 1999), and Aiyutaka and Touya, which are progenies of Gpa2 donor S. tuberosum ssp. andigena CPC 1673, were used. For Gro1-4, Alwara which possesses Gro1-4 (Biryukova et al. 2008) and W872204, W872204-1 and W872204-2, which are progenies of S. spegazzinii, were used. The PCR condition for GpaIVSadg was determined with Eden, which is known to have GpaIVSadg (Moloney et al. 2010) and Irida, P10111-1, W804421-3 and W862208-1, which are progenies of S. tuberosum ssp. andigena. Because we do not have varieties and lines known to have Grp1 and RGp5-vrnHC, we chose, Astarte, Elles, Magic Red, Mara and Starter, as they are progenies of several wild species, and show broad-spectrum resistance. Irish Cobbler and May Queen, which are considered to have no PCN resistance genes, were always used as PCN-susceptible genotypes. Because Marijke, Alwara and Eden do not exist in Japan, DNA of these varieties were provided by Dr. John Bamberg in USDA, ARS, University of Wisconsin.
DNA markers used in this study are shown in Table 1. Although Gpa2 has already been isolated and well characterized, DNA markers only linked to Gpa2 have been reported (van der Voort et al. 1997, 2000). Therefore, we developed DNA markers directly amplifying Gpa2. Gpa2 and its related genes (RGC1, RGC3, PSH-RGH6, PHS-RGH7 and Gpa2-like NBS-LRR protein) (van der Vossen et al. 2000) were aligned using CLUSTALW, followed by manual alignment. We developed 2 markers (Gpa2-1 and Gpa2-2) amplifying specific regions of Gpa2. In addition to previously published marker, Gro1-4, we developed a more specific marker, Gro1-4-1, by aligning nucleotide sequences of Gro1-4 and genes of the Gro1 family (Gro1-1, Gro1-2, Gro1-3, Gro1-5, Gro1-6, Gro1-8, Gro1-10, Gro1-11, Gro1-12 and Gro1-14) (Paal et al. 2004) using CLUSTALW.
The total volume in the individual PCR assays was 10 μL, including 2 μL template DNA, 5 μL gene amplification reagent (Ampdirect® Plus, Shimadzu Corp., Kyoto, Japan), 0.25 units Taq DNA polymerase (BIOTAQTM HS; Bioline, London, UK) and the related primer pair shown in Table 1. Thermal cycling for Gro1-4-1, Gpa2-1, Gpa2-2 and GBSS1-3 was performed by using a 96-well thermal cycler (Veriti®; Applied Biosystems, Life Technologies, Carlsbad, CA). The PCR conditions consisted of 1 cycle of 10 min at 94°C, followed by 35 cycles of 30 s at 94°C, 30 s at 60°C and 1 min at 72°C and finally, 1 cycle of 5 min at 72°C. The PCR conditions for Gro1-4, HC, N146, N195, TG432 and C237 were as previously described (Finkers-Tomczak et al. 2009, Moloney et al. 2010, Paal et al. 2004, Sattarzadeh et al. 2006, Takeuchi et al. 2008). PCR products of TG432 and C237 were digested with restriction endonucleases RsaI and TaqI, respectively. All PCR products were separated by electrophoresis on 1.2–2% agarose gels in 1 × TAE buffer and visualized with SYBR SafeTM DNA gel stain (Invitrogen, Life Technologies, Carlsbad, CA) and UV.
N146 and N195 amplified expected-size bands (506 and 337 bp) in resistant varieties, Kita-akari, Touya and Haruka but not in Irish Cobbler and May Queen (Fig. 1A). Further, Gpa2-1 and Gpa2-2 amplified the expected-size bands (1120 and 452 bp, respectively) in Marijke, Touya and Aiyutaka but not in Irish Cobbler and May Queen (Fig. 1B). The expected-size band (602 bp) was amplified using published marker Gro1-4 only in Alwara; however, a slightly larger band was amplified in W872204 and W872204-2 (Fig. 1C). As the size difference is very slight, there is possibility that varieties or lines that have the larger band might be falsely judged as positive for Gro1-4. To avoid such erroneous decision, we developed a new PCR marker that specifically amplify Gro1-4 as described in Materials and Methods. The newly developed marker Gro1-4-1 amplified the expected 602-bp band only in Alwara (Fig. 1C). TG432, CAPS marker for Grp1, was tested with five resistant varieties (Astarte, Mara, Elles, Magic Red and Starter). However, we could not determine which band correlated with resistance (Fig. 2A). For the CAPS marker C237, Irida, P10111-1 and W804421-3 showed the same band pattern as positive control Eden; however, the negative control May Queen also showed the same pattern (Fig. 2B). HC did not yield a stable result; positive bands were also amplified in Irish Cobbler and May Queen irrespective of the PCR condition (Fig. 2C). Therefore, we finally used N146, N195, Gpa2-2 and Gro1-4-1 for evaluating the genetic resources.
To avoid erroneous judgment by PCR errors, a marker amplifying granule-bound starch synthase 1 (GBSS1), GBSS1-3, was combined with N146, N195, Gpa2-2 and Gro1-4-1. GBSS1 is shared in all potato species (Spooner et al. 2008), thus, it functions as a positive control to check whether the PCR was performed correctly (Mori et al. 2011). DNAs isolated from Touya (H1 and Gpa2) and Alwara (Gro1-4 and Gpa2) were equally mixed (DNA mixture), and was used as samples. The multiplex PCR conditions were optimized by varying the primer and Taq DNA polymerase concentrations and annealing temperatures. Consequently, simultaneous amplification was achieved with the primer concentrations shown in Table 1, 0.5 units of Taq DNA polymerase and annealing temperature at 60°C (Fig. 3).
To determine detection sensitivity of the multiple PCR method, the DNA mixture and DNA isolated from Irish Cobbler were assayed by the multiplex PCR method for a series of template DNA concentrations (60, 40, 20, 10, 5, 2.5, 1.3 and 0.6 ng per 10 μL reaction). In the DNA mixture, all marker bands were detected at 1.3 ng or higher concentrations, and Gro1-4-1 was not detectable at 0.6 ng. In Irish Cobbler, GBSS1-3 band was detected at concentrations as low as 0.6 ng (Fig. 4).
The DNA concentration of 30 randomly selected samples ranged from 3.1 ng/μL to 41.7 ng/μL, with an average of 14.1 ng/μL (SD = 9.1). Because the DNA concentration of the smallest sample was much higher than the detectable range, 2 μL of these DNAs were used as template DNA in a 10 μL PCR without adjusting the concentration.
The genetic resources were evaluated by the multiplex PCR method. As expected, N146 and N195 co-segregated perfectly. Of 812 varieties and lines, 264 varieties and lines were positive for the H1 markers (Table 2). Especially, these markers were detected in almost all varieties for which breeding program started after PCN invasion of Japan in 1972 (Table 3). We found 142 varieties and lines positive for the Gpa2 marker (Table 2). Of these, 117 varieties and lines were positive for both H1 and Gpa2 markers (Table 2). Among the varieties and a line mainly used as Japanese H1 resources (Tunika, Atlantic and R392-50), the Gpa2 marker was also detected in Atlantic and R392-50 but not in Tunika (Table 3, Fig. 5 and Supplemental Table 1). No accession was positive for the Gro1-4 marker (Table 2). None of the 3 markers were amplified in varieties such as Astarte, Mara and Magic Red, which are categorized as PCN resistant in The European Cultivated Potato Database or breeder’s evaluation (Supplemental Table 1).
As expected, one-third of the varieties and lines in our genetic resources possess H1. Especially, most varieties recently bred in Japan possess H1 (Tables 2, ,33 and Supplemental Table 1). Because Ro1 resistance is the priority for potato varieties in Japan, many varieties and lines inevitably have H1. Interestingly, many varieties and lines also possess Gpa2, although no selection for G. pallida resistance has been performed. Both H1 and Gpa2 were originally derived from the same S. tuberosum ssp. andigena CPC 1673 accession (van der Voort et al. 1997). Atlantic (Wauseon × Lenape) and R392-50 (Hudson × Wauseon) both having Gpa2, probably derived from Wauseon, and their progenies are frequently used for breeding of recent resistant varieties. Their frequent use might have resulted in the presence of Gpa2 in many recent varieties and lines. Alternatively, Gpa2 is closely located (less than 200 kb apart) to the Potato virus X (PVX) resistance gene Rx1, and therefore, these genes are genetically linked (van der Vossen et al. 2000). Along with PVX resistance, Gpa2 might also have been unconsciously introduced into many varieties. In fact, all varieties and a line with Rx1 (Aiyutaka, Atlantic, Kintoki-imo, Saco, Sayaka, Touya and Chokei 108) (Ohbayashi et al. 2010), also have Gpa2 (Supplemental Table 1).
In contrast to Gpa2, we could not find varieties and lines having Gro1-4, although our samples included PCN-resistant S. spegazzinii (PI 275143), a different accession of the S. spegazzinii and its progeny (W792224-1) (Supplemental Table 1). This fact emphasizes the importance of introduction of varieties or lines having Gro1-4 with a priority for our future safety. Our results revealed that only a very narrow genetic background in regard to the potato cyst nematode resistance resource has been used in Japanese potato breeding. Such a narrow genetic background of PCN resistance resources is disadvantageous from the viewpoint of breeding of novel PCN resistance varieties, because this may cause a bottleneck effect in the genetic background, and may result in the eventual genetic vulnerability of crops to the pest. Besides the 3 resistance genes analyzed in this study, we found other resistance genes in foreign varieties which are categorized as PCN resistant in the database and breeder’s evaluation. Their utilization and breeding of other novel resistant varieties should be promoted to broaden the genetic background in Japanese potato breeding. However, reliable DNA markers to detect these resistance genes have not developed yet. In fact, DNA markers TG432, C237 and HC were not applicable to the evaluation of resistant accessions in our study (Fig. 2). This is probably because of the complex genetic background of potato as pointed out by Milczarek et al. (2011). They tested the suitability of published molecular markers and suggested that most markers are inapplicable because of complex genetic backgrounds (Milczarek et al. 2011). DNA markers that accurately identify these resistance should be developed for efficient breeding.
Application of DNA markers for breeding has numerous advantages such as time saving, consistency, biosafety, efficiency and more accurate selection of complex traits (Collard et al. 2005, Jena and Mackill 2008). In this study we focused on the biosafety to prepare for PCN pathotypes not yet invading Japan. Usually, breeding programs target resistance to diseases and pests that are already epidemic in the region. In such cases, DNA markers are often used to reduce cost and time, to precisely and effectively evaluate complex traits and to simultaneously evaluate several resistance genes and pyramiding them. In fact, several studies that use marker-assisted selection target diseases already found in the area, and use DNA markers for such purpose (Biryukova et al. 2008, Gebhardt et al. 2006, Mori et al. 2011, Tan et al. 2010). To the best of our knowledge, this is first attempt in potato breeding to use DNA markers for preparations to prevent diseases and pests from invading the country. If functions of other gene/genes such as complementary gene are essential for expression of the resistance, some varieties selected based on only DNA markers evaluation may not show resistance. Therefore, it is necessary to confirm whether some of the candidate varieties and lines show resistance by inoculating other pathotypes, but no other pathotypes exist in Japan at this time. However, if we breed varieties possessing resistance genes, we will be able to choose resistance varieties among them when other pathotypes invade. These varieties enable us to quickly respond to invasion by other pathotypes. Our study demonstrates that DNA markers can be used to select potentially resistant accessions in potato breeding before infestation by new pathogens. Indeed, in addition to G. rostochiensis Ro1 resistance, we found many varieties and lines possibly resistant to distinct populations of G. pallida. This approach may be applicable to other quarantine diseases such as Columbia root-knot nematode and potato wart, DNA markers of which resistance have already developed (Gebhardt et al. 2006, Zhang et al. 2007). Moreover, because we evaluated many varieties used in potato breeding in Japan and several major varieties in the world, evaluation data in this study are very informative and useful for breeders. Breeders can devise schemes to breed novel resistance varieties based on these data. Furthermore, the multiplex PCR method developed in this study will contribute to efficient selection of ideal genotypes and facilitate breeding of novel resistant varieties.
We thank Dr. J. Bamberg in USDA, ARS, University of Wisconsin for providing DNA samples. We are also grateful to N. Taira, T. Yamada and A. Morizumi for field maintenance, S. Soma, H. Suzuki, H. Onuma, K. Okamoto, M. Okada and H. Ozaki for technical assistance and Dr. K. Hosaka and Dr. M. Mori for helpful suggestions. Almost genetic resources used in this study were distributed by the NIAS Genebank Project of the National Institute of Agro-biological Sciences.

domain: agronomy


text:
Our latest exclusive Q+A is with Jimmy Cliff on the rocksteady legend’s recent collaboration with Rancid’s Tim Armstrong, history with The Clash (more on that below), and iconic role in 1972’s The Harder They Come. Tomorrow, get ready for PJ Harvey.
GQ: How did you hook with Strummer in the first place?
Jimmy Cliff: I and the Clash used to cross each other’s path when we did concerts in Europe. We never had time to like, stop and talk, but we’d just say, “Hey Joe!” “Hey, Jimmy. What’s up, what’s going on?” “All right. See you.” It was always more or less like that. Like a respectful artistic nod to each other, you know. So when he came into the studio while I was doing the Black Magic album—it had two titles, Fantastic Plastic and Black Magic—I thought he just came in to greet me. We sat down talking a little about music, and he came out with these lyrics and said, I hear you singing these. He showed them to the producer who said, Well how does the melody go? And he said, I don’t have any melody! I just have these lyrics and I hear Jimmy singing them. So we three of us sat down and put the melody together. It was really a great track.


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Key features and details
- Rabbit polyclonal to CalDAG-GEFI
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CalDAG-GEFI antibody
See all CalDAG-GEFI primary antibodies
DescriptionRabbit polyclonal to CalDAG-GEFI
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig
Recombinant fragment corresponding to Human CalDAG-GEFI aa 299-609.
- MOLT4, Raji and Mouse liver whole cell lysates.
Protein previously labeled as RASGRP2.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 69 kDa.|
FunctionFunctions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.
Tissue specificityDetected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney.
Sequence similaritiesBelongs to the RASGRP family.
Contains 2 EF-hand domains.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-GEF domain.
Developmental stageExpressed in fetal brain, lung, liver and kidney.
DomainThe N-terminal Ras-GEF domain mediates association with F-actin.
modificationsIsoform 2 is palmitoylated and myristoylated while isoform 1.
Cellular localizationCytoplasm > cytosol. Cell membrane. Cell junction > synapse > synaptosome. Cell projection > ruffle membrane. Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons.
- Information by UniProt
- Calcium and DAG-regulated antibody
- Calcium and DAG-regulated guanine nucleotide exchange factor I antibody
- Calcium-and diacylglycerol-regulated guanine nucleotide exchange factor I antibody
ab137608 has been referenced in 3 publications.
- Vanshylla K et al. Grb2 and GRAP connect the B cell antigen receptor to Erk MAP kinase activation in human B cells. Sci Rep 8:4244 (2018). PubMed: 29523808
- Edelmann B et al. JAK2-V617F promotes venous thrombosis through ß1/ß2 integrin activation. J Clin Invest 128:4359-4371 (2018). PubMed: 30024857
- Tang S et al. RasGRP3 limits Toll-like receptor-triggered inflammatory response in macrophages by activating Rap1 small GTPase. Nat Commun 5:4657 (2014). WB ; Mouse . PubMed: 25118589

domain: health


AUTO-ENTREPRENEURS have had a last-minute reprieve from a new tax that could have seen some people with tiny incomes, or none, pay up to €2,000.
A body representing people registered under the simple business regime has negotiated a change in the law with Frédéric Lefebvre, the small busness minister.
The president of the Fédération des Auto-Entrepreneurs (FEDAE), Grégoire Leclercq, said: “Parliament has rectified the law, with retroactive effect for 2010, which allows for all auto-entrepreneurs to be exonerated. We are very pleased. We were alone in this fight.”
Auto-entrepreneurs who started their businesses in 2009 (the first year of the regime) were being asked to pay cotisation foncière des entreprises (CFE, a new replacement for the professional tax, TP) by mid-December 2010, unless, when they set up, they had chosen an optional income tax system, the micro-fiscal. Even those who had registered a business but had no turnover were originally expected to pay.
While all auto-entrepreneurs pay no CFE in the year of setting up, now all of them will also benefit from a three-year exoneration available to those on the micro-fiscal.
What is more, before 2013, when the first payments will now fall due, the FEDAE hopes to negotiate a change in the way the CFE is calculated, making it proportional to turnover. Currently it is based on the theoretical rental value of the building where you work (usually the home for auto-entrepreneurs) to which a rate set by mairies is applied.
While it is possible to state that you only use a set portion of your home for work, councils set a minimum on the tax levy, which FEDAE say can vary from €200 to €2,000.
FEDAE believes making the tax proportional to turnover will be more in the spirit of the auto-entrepreneur, of which one main element is social charges paid at intervals throughout the year based strictly on turnover (and an option to pay income tax in the same way, the micro fiscal).
Many autoentrepreneurs are retired or have a salaried job, and set up small businesses for extra money on the side.
Mr Leclercq said: “The new tax is similar to the TP, but when they created the auto-entrepreneur regime, the government said, ‘Forget what was done before; we’ve created something new for you’. The whole philosophy was: you have no turnover, you have nothing to pay; you have a bit of turnover, you pay in proportion to it.”
FEDAE argued that the new tax, as originally planned, would kill off the auto-entrepreneur scheme, which has seen about 600,000 sign up, encouraged by simpler-than-usual administrative procedures, such as being able to register the business online. Many people did not take up the micro-fiscal, especially if they fall below the income tax threshold and expected to stay under it with a small income as autoentrepreneurs.
People doing an “artisan” type job of a mainly manual kind are permanently exonerated from CFE (these only make up 12 per cent of auto-entrepreneurs).
To qualify, your business must also not use very “sophisticated” equipment requiring a large capital outlay and it should not be based on “speculating on raw materials”, thus, ruling out bakers or butchers, according to APCE, the official entrepreneurship agency.


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Brazil's Lula, a high-school dropout, closes an exceptional era
Brazilian President Luiz Inácio Lula da Silva will step down tomorrow with an 87 percent approval rating, though some say he failed to make necessary long-term economic reforms.
| São Paulo, Brazil
One of the best-selling books in Brazil this year was “Never In This Country’s History,” an acerbic but humorous look at the memorable phrases uttered by Brazilian president Luiz Inácio Lula da Silva during his eight years in power that will come to a close tomorrow.
The book’s title is in reference to what has become one of Lula’s catchphrases. With characteristic hyperbole, Lula frequently boasts about his successes. No one has ever done as much to help Brazilians or transform the country as the high school drop out turned president and statesman.
Or as he said in 2003, according to one quotation from the book: “I arrived at the presidency to do the things that needed to be done and that many presidents before me were cowards and didn’t have the courage to do.”
Olympic and World Cup host
No one would argue that Lula has a high opinion of himself. But then again, he has reason to be proud of his achievements. When he took over in January 2003, Brazil’s economy was floundering, with the currency falling, interest rates rising, and the IMF standing by with a record $30 billion in bail out funds.
In spite of having little economic education, Lula used common sense to turn the country around. He stuck to his predecessor’s policies where appropriate and innovated where necessary.
Eight years on, the currency is strong (perhaps too strong), interest rates are half what they were, and direct foreign investment has tripled. In addition, Brazil has assumed a once unimaginable importance on the world stage. It won the bid for the 2016 Summer Olympics over Chicago, among other cities, and will host the 2014 World Cup as well – a nice catch for Lula, who is a big fan of football.
Rise of the middle class
Most important, Lula has done what few Brazilian presidents have managed – or even attempted – and narrowed the shocking gulf between rich and poor.
Thanks mostly to his expansion of the Bolsa Familia (or Family Aid) program, 24 million Brazilians have left absolute poverty and 31 million have progressed into the middle class.
The definition of middle class is low by US standards – a family earning between 1,115 and 4,807 reais a month ($660 and $2,844) is considered middle class – but it is enough to make them into the consumers who are driving the country’s booming economy.
Going out with approval rating of 87 percent
That said, there is still a huge amount of work to be done and one of the most salient criticisms of Lula is that he has consistently overlooked or downplayed the country’s problems.
Educational standards remain chronically low and corruption is endemic. The government machine has expanded exponentially and although Lula has a personal approval rating of 87 percent, he made no attempt to use clout to make the structural reforms that many economists say are necessary for the country’s long-term development.
Lula, like most politicians, refuses to acknowledge he has made mistakes. But one thing is for certain. Never in this country’s history has Brazil seen a president like Lula and it may never again. Which would be unfortunate for many reasons, the least of which is his ability to give a good quote.
He will be missed.

domain: news


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The Michigan Department of Transportation in the past six months has resumed its feasibility study of an east-west thoroughfare linking I-75 to I-275 in southern Wayne County.
The link, which would be called M-2, is expected to stretch seven miles, with the option of a possible extension to U.S. 23.
M-DOT is determining the viability of extending an existing east-west road to create the link. In April 1989, the Transportation Department selected West Road, but department officials said they are considering other options, including Pennsylvania and Sibley roads.
M-DOT has considered creating an east-west corridor in the Downriver area since 1982, but the concept was shelved in 1992 because of budget constraints.
``We've begun looking at what was previously done in terms of environmental clearance and agreements with Wayne County and the (Huron-Clinton Metropolitan Authority) that have been reached,'' said David Wresinski, manager of project planning for the Transportation Department.
``We need to see what we need to do to make these things current in terms of the federal process.''
The department is investigating the advantages and disadvantages of the proposed linking roads.
``We need to know if there are any wetlands or if there would be any environmental impact or if the road is the right width,'' said James DeSana, Transportation Department director.
M-DOT also will determine whether or not the state would have to buy any property and at what cost. The project is in its preliminary phase, and there is no cost estimate or target date for construction.
John Roach, a spokesman for the Wayne County Department of Public Services, said the chosen road could be turned into a state trunk line, like Telegraph Road or Michigan Avenue. Also as a part of this study, M-DOT is considering building a bridge over the Huron River, cutting through Willow Metropark.
``We have agreement with the Huron-Clinton Metropark to allow us to build a bridge over the park on an extension of West Road,'' Wresinski said. ``But the development of that bridge is not the determining factor of what alignment we pick.''
He said he expects the project's preliminary stage to be completed in late winter or early next spring.
After that, the Transportation Department would move on to the actual design of the project.


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Restaurant quality edamame at home? Yes please! Say hello salty-sweet perfection this spicy garlic Edamame. The sticky sauce sticks nicely to each capsule for a delicious taste in each bite.
What is Edam?
In Japan, Edam refers to a popular appetizer or side dish made by bleaching young soybeans. The soybeans are removed from the pod by the teeth, and the rest of the shells are thrown away. A similar dish is served in China և Taiwan, where it is called maodu (source:):
The reason for the green color of the edema (instead of beige like dry soybeans) is that the edema pods are collected until they are fully ripe. Choosing them early means they are sweeter, more definite nutrients: like folic acid և vitamin K. Edam is also rich in protein and contains all the essential amino acids.
How to cook spicy garlic Edamam
This: easy The recipe starts with frying red pepper flakes of garlic և ginger in coconut oil (or another oil). Infuse the oil with spicy, garlic goodness.
Then add tamar և maple syrup: a sweet և delicious match made in heaven. Then the mixture of archery starch and water enters, and when heated, it turns into a mixture. thick և sticky sauce.
Next, we add: boiled or steamed edamam Mix the capsules so that they are covered with an aromatic sauce. And if desired, mix the fried sesame oil for extra flavor.
The result is: tender, spicy capsules ready to swallow. When you are ready to eat the edam, grab the pod with your fingers and remove the beans with your teeth. Discard the beans after eating the beans.
We hope you LOVE this Edam. That.
A savory appetizer
& Amazingly delicious!
We like to serve it as an appetizer or side dish, especially in combination with similarly flavored dishes, such as Cauliflower by General Joe or: Crispy Miso Chickpea Bowls with Garlic Sesame Sauce. It’s delicious too! mix-fry, fried riceor: noodles!
More plant-based snacks
If you try this recipe, let us know. Leave a comment, rate it և do not forget to mention the photo @minimalistbaker: On Instagram. Congratulations, friends!
Services 4: (~ 3/4-cup portions)
- 16: ounces with frozen edamame pods
- 1/4: cup water
- 1/2: ճ.գ: arrowroot starch:
- 1: ճ.գ: coconut oil (or sub-avocado oil)
- 2: cloves garlic, finely ground or grated
- 1: ճ.գ: fresh ginger, finely ground or grated (~ 1 inch piece)
- 1/2 – 1: ճ.գ: red pepper flakes
- 1 ½: ճ.գ: Tamar (Provide gluten free if needed)
- 1: ճ.գ: maple juice
- 1/2: ճ.գ: fried sesame oil (optional)
Cook the adamam according to the package instructions in the microwave (covered), stew or boil, all working equally well. We boiled our edamam in 3 liters of salted water for րոպե 8 minutes, squeezed it and set it aside.
While the edama is cooking, add the water: starch to a small mixer or measuring cup. Stir until the starch dissolves. Put aside.
Melt the coconut oil in a stainless steel or non-stick pan over medium-low heat. After melting, add garlic, ginger և red pepper flakes. Fry for 1-2 minutes, until fragrant and slightly turbid, but be careful not to burn the garlic, otherwise it will become bitter.
Add tamarind և maple syrup և mix until evenly combined, then add the archer / water. Bring this mixture to a boil, then reduce the heat and cook for 2-3 minutes, until the sauce thickens and sticks.
It is best in fresh condition, but it is stored in the refrigerator for 2-3 days. Heat in the microwave or covered և in a frying pan. Not suitable for refrigeration.
* Nutrition information is an approximate estimate calculated without selective components.
Serving: 1: (3/4 cup serving) Calories: 207: Carbohydrates. 17.3: is: Proteins: 14.9: is: Fat: 10.5: is: Saturated fats. 3.5: is: Polyunsaturated fats. 0: is: Mono unsaturated fats. 0.2: is: Trans fats. 0: is: Cholesterol: 0: mg: Sodium. 442: mg: Potassium. 641 mg: Fiber: 4.4: is: Sugar: 6: is: Vitamin A: 175: IU: Vitamin C: 17: mg: Calcium. 151: mg: Iron: 2.7: mg:
see secret product in Box below
‘The accuracy or reliability of any information/material/calculation contained in this article is not guaranteed. This information has been brought to you by collecting from various mediums / astrologers / almanacs / discourses / beliefs / scriptures. Our purpose is only to deliver information, its users should take it as mere information. In addition, any use thereof shall be the responsibility of the user himself.’

domain: food


text:
I'm a mountain girl. I'm not a water girl. Give me a hike in the mountains, through the forest, and around the lake over a swim in the ocean or a day lying around at the beach any day.
There are exceptions to my general mountain over ocean preferences: an ultimate game on the beach is preferable to a death march hike through the Grand Canyon, for example. For the most part, though, mountain over ocean.
For some reason I'm not quite clear on, I keep ending up at the ocean at these ultimate tournaments. Nationals was in Sarasota, Florida (a gulf more than an ocean, but a large body of water nonetheless), Worlds is in Perth, Western Australia.
So, in Perth, we're staying (ick! mixing verb tenses!) at a beach apartment complex, across the street from the beach, so all of maybe 50 yards from the waterfront. We wandered across the street to the beach,
"It's cold. I don't want do it, but I want to have done it." I smiled and said, "Well, the only way to have done it is to do it. Let's go."
The water was cold, close but not quite unbearably so, so we rushed out into the water, letting the waves crash higher and higher up our bodies. Eventually Kris went under, and a few waves later, I followed. I didn't go completely under, so chose to ride the next wave back towards shore.
I pushed off poorly on the next wave, but started swimming as fast as I could toward shore. Unfortunately, I also started after the wave started crashing. Instead of getting an easy ride to shore, I was dumped under the water, with the wave crashing over my head.
As I went under and couldn't find my feet under me, I kept thinking of my scuba diving qualification dive at Zuma Beach, and felt the brief tickle of panic that accompanies that memory when I'm in the water. Before it could become panic, my feet struck sand, and I surged forward, running to the shore.
I can now say, I have swum in the Indian Ocean. Kris can say the same.
I suspect I'll be heading back in the next few days, though. Once doesn't seem quite enough.


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New wholegrain labelling system helps consumers choose healthy breakfast options
University of Hertfordshire researchers have been trialing a new approach to help the public easily identify which wholegrain foods are healthiest.
Currently there is no national or international definition of what a wholegrain food is, leading to some foods containing high levels of fat or sugar being labelled as wholegrain, particularly breakfast cereals, and appearing to be healthier than they are.
Their findings have been published in the online journal Public Health Nutrition and could be used by food manufacturers to develop a new food labelling system in addition to the current traffic light system.
The UK Institute of Grocery Distribution recommend that a food should have at least 8g of wholegrain ingredients per serving to be called whole grain. However, this recommendation is non-binding and the content of other nutrients are not specified in relation to this resulting in some foods, currently described as wholegrain, also having high levels of sugar or fat.
Researchers Bahar Ghodsian and Angela Madden have found that wholegrain foods with a carbohydrate: fibre ratio of less than 10:1 tend to be the lowest in fat, sugar and salt. A total of 266 breakfast cereals and 162 breads sold at the four major UK supermarkets were identified as meeting this criterion and their nutritional quality was compared with the Food Standards Agency traffic light system. Breads which met the criterion typically contained medium fat, low saturated fat, low sugar and medium salt whilst breakfast cereals typically contained medium fat, low saturated fat, high sugar and low salt.
The benefits associated with eating wholegrain ingredients are well-established and include reducing the risk of cardiovascular disease (CVD), type 2 diabetes and many cancers. However, wholegrain intake in the UK remains low with 45% of the population eating less than one serving per day and 18% eating none over a four-day period.
New wholegrain labelling system
Dr. Angela Madden, Lead for Nutrition and Dietetics at the University of Hertfordshire who supervised the project, said "In the UK, the average intake of sugar is higher than recommended and average intake of fibre is less than recommended. There is lots of information in the media about sugar but people often forget about fibre and it is important to consider both of these, especially in foods like breads and cereals.
"A public health initiative is clearly needed to substitute foods containing whole grains for those containing significant amounts of refined grains in order to improve health, save resources and reduce mortality."
"Our work shows that using the carbohydrate:fibre ratio helps to identify foods that have good nutritional value. It supplements the existing front-of-pack traffic light labelling currently in use. We suggest that food manufacturers should consider adopting this approach to help consumers."


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Each and every serving of Instantized BCAA 5000 Powder provides a 5,000 mg (5 g) blend of the three Branched Chain Amino Acids (L-Valine, L-Leucine, and L-Isoleucine) in each serving. The Branched Chain Amino Acids (BCAAs), so named as a result of their branched chemical structure, are very important in that the body can’t lead them to from other compounds. The option to produce Instantized BCAA 5000 Powder yields a powder that mixes readily in water or juice and does not readily clump or settle to the bottom. In consequence, the trouble in mixing you might have experienced with other BCAA powders is greatly reduced.
Easy mixing formula
Blend of the three branched chain amino acids

domain: chemistry


MELBOURNE, Australia, March 17, 2006 - The Melbourne 2006 Commonwealth Games is distributing real-time information to more than 35 venues across a data communications network provided by Cisco Systems®. The network, based on the Cisco® integrated services router, is providing critical data to more than 2,000 desktops for the largest temporary network in Australia since the 2000 Sydney Olympic Games.
Cisco's Australia and New Zealand managing director, Ross Fowler, said; "Cisco is delighted to be helping to provide real-time information for this internationally celebrated sporting event. We are also pleased to be extending our relationship with the Victorian State Government, the official host of the Commonwealth Games, as a trusted provider of intelligent networking solutions across a growing list of government initiatives and departments."
As the official provider of information technology (I.T) network hardware for routing and switching to the Melbourne 2006 Commonwealth Games, Cisco is working closely with Games organisers, and systems integrator Australian Project and Consulting Services (APCS), to help ensure the smooth running of the Games for thousands of users by carrying scoring, timing and results data generated by competition venues. The Melbourne 2006 Games Data Network (GDN) connects more than 2,000 desktop computers, 900 printers and almost 100 servers across all 35 Games venues. The GDN uses 215 Cisco Catalyst switches and more than 50 Cisco integrated services routers to support management systems, information services and printing functions across non-competition venues, including the athletes' village, the media centre and administrative offices.
The GDN has been fully operational for a number of months in the lead-up to the Games. During this time, Cisco's SMARTnet maintenance service has ensured smooth day-to-day network operation by guaranteeing priority access to equipment support and parts replacement. Throughout the network construction period, Cisco Advanced Services engineers provided a detailed network design audit review to ensure a highly available and stable network.
Brian Nourse, Group Manager, Technology, at the Melbourne 2006 Commonwealth Games Corporation, said; "Athletes, officials and the media rely on the delivery of critical real-time information throughout the Games. This requires an extraordinarily robust and stable network with guaranteed 100 per cent uptime. We needed to feel confident we were using tried and proven technology with excellent local support and we are getting exactly that from Cisco and APCS."
The Melbourne 2006 Commonwealth Games commence on March 15. For further information, see www.melbourne2006.com.au.
APCS Managing Director Scott Coleman said, "Cisco's best-of-breed technology and great technical support have helped us realize the core design goals of the Games Data Network: superior performance and extraordinary resilience."


text:
Skip to code content (skip section selection)
(A) Operation of vehicles; pedestrians; bicycles. Users shall travel on or occupy only those area designated for that particular class of travel. The Airport Director may establish, with the advice of the Airport Board, policies, guidelines and procedures to ensure safe traffic flow on airport property.
(B) Speed limits. Vehicles at the airport shall be operated in strict compliance with posted speed limits. The prevailing posted speed limit for ramp areas is 15 mph.
(C) Loading or unloading passengers. Owners and operators of vehicles shall load and unload passengers in a safe and reasonable manner. FAA Part 121 operators may load and unload passengers only in designated areas.
(D) Vehicles in sound mechanical order. All vehicles on airport property must be operational and licensed except as follows: vehicles operated by air carriers and fixed base operators during the course of commercial activities on the airport need not be licensed. Aircraft tugs, golf carts and similar personal transportation vehicles need not be licensed.
(E) Repair of motor vehicles. Users shall clean, repair and/or overhaul vehicles only in authorized areas designated by the Airport Director.
(1976 Code, § 14-6) (Ord. 552-09, passed 10-22-2009)


text:
Michelle Austin’s Trans Men Adventures brings trans men into the world of Michelle Austin. She goes on an adventure to have sex with some of the hottest trans men around. Each scene has its unique theme, with tons of passion and hot action. She is in a 1950s scene called Mr & Mrs Beaver with porn icon James Darling, has a hot workout with Chance Armstrong in A Chance Workout, she gets in trouble in her college dorm with Fritz Von Fuckup in Dorm Room Fuckup and she introduces the world to Parker Reed in Grindr Hookup. Some of the hottest sex between a trans woman and trans man!
Citation: Michelle Austin's Trans Men Adventures, USA, 104mins, 2014
Performer(s): Chance Armstrong, Fritz Von Fuckup, James Darling, Michelle Austin, Parker Reed.
Director(s): James Darling, Michelle Austin.
Producer(s): FTM Fucker, Kennston Productions.
Studio(s): Michelle Austin Films.
Tags: anal, ftm, hardcore, kissing, mtf, oral, tboy, tgirl, trans, vaginal

domain: library


text:
found a new home!
Plenty of my friends are looking
for one too. Find a pet to adopt.
I am already neutered, housetrained, and up to date with shots.
He lost most of his teeth from dental disease, but still has some in the back and still likes to chew on nylabones :-) He also loves playing with soft toys. He is ok with other dogs, but would be happy getting all the love and attention! He is about 8 years old.
For more information, please contact firstname.lastname@example.org.
If you are interested in adopting, please complete our application on our website at: norcalcockerrescue.org

domain: pet


text:
Winter Garden – Nothing You Can Do In This
Disclosure: This post may contain affiliate links from which I earn a commission.
The weather outside is frightful… at least it is in my front garden! Batton down the garden hatches, it is -7 degrees with a windchill of -30 in the Chicagoland area.
My best suggestion: hot cocoa and the love of your family. Enjoy!
Shawna Coronado says Get Healthy! Get Green! Get Community!
Peace on Earth Right In My Back Yard
you are fantastic and rocking

domain: hobby


text:
› Benefits of taking SB 5 test
Benefits of taking SB 5 test
posted by Karen Quinn, The Testing Mom - September 22nd, 2011
If you want your child to benefit greatly from the Stanford-Binet 5 test, you can make use of free practice IQ tests for children. Whether your child is in kindergarten or elementary, having them engage in practice tests for the SB 5 will come in handy. The questions asked on this site are somewhat similar to the actual SB 5 exam but focus on the concepts. This test been designed to evaluate an individual’s mental processes which include how he recalls information, how they reason, as well as understand the world around them. It is also especially helpful for those who have autism, are deaf, or may have difficulties with learning and communication.
We have free SB 5 sample questions on TestingMom.com to see how your child preforms. Through the questionnaire, you can go over the items with your child to help them prepare ahead of time. It will also allow you to identify any problem areas that your child may have early on, and since the actual SB 5 test is individually administered by a psychologist, you can communicate the results of the practice exam to them. All you need to do is go to “how it works” at the top of this website, register, and sign up for the package at a very affordable price.

domain: psychology


text:
Single Diamond Stud 14ct Gold - 2 Sizes
Say hello to the sweetest diamond stud you'll ever see. This petite earring feature a 1.5mm or 2mm conflict free diamond in a classic 4 claw 14ct yellow gold stud setting. These studs come with a matching 14ct butterfly back.
Please note these earrings are sold as singles, if you would like a pair, simply add two to your basket. Second image shows the Tiny Stud next to the Small Stud.
Size: 1.5mm or 2mm diamond
Materials: Conflict-free FG/S1 diamond, 14ct Yellow Gold
Care: To keep your jewellery shining bright, gently rub with a polishing cloth.
For more information about materials, jewellery care, shipping and returns, please visit our FAQs page.
As with all of our handmade jewellery, small differences in finish are expected and add to the charm of the piece. The images shown may differ slightly, especially with hammered and organic textured pieces.
About Handmade Jewellery
Materials & Care

domain: design


By Bongani Ndlovu
Selmor Mtukudzi, who had been left out of her late father’s birthday bash line-up, has now been included, likely due to public demand.
Ever since her father, Oliver, died, music lovers have developed an inclination for Selmor who has been trying hard to keep her father’s legacy alive. As such, fans have been demanding that she be included in all concerts held in honour of Tuku judging by the uproar each time she is left out.
Chipaz Promotions, organisers of the Tuku Memorial Concert which will also serve as a birthday bash for the artiste who would have turned 67 on September 22, said they had initially left Selmor out because she had another booking on the same day — September 27. But now that booking has been cancelled and she has been drafted in.
Patson Chimbodza of Chipaz Promotions said: “Selmor is now part of the show to celebrate the life of her father, the late Dr Oliver ‘Tuku’ Mtukudzi.
“Previously when we approached her, she said she was booked on the day. However, as fate would have it, she’s now part of the line-up as the show she had been set to perform in South Africa has been cancelled”.
Selmor was supposed to perform in Cape Town next Friday and Johannesburg next Saturday as part of her legacy concerts. According to her, the shows in South Africa have been cancelled because of the xenophobic violence in that country.
Chipaz said the addition of Selmor was a welcome development as it is a boost for the show.
“Selmor being on the line-up adds more weight to the show. Firstly, we’d like to commend her for agreeing to perform for free as this is a family (Tuku family) show. Secondly, her being on the line-up means that her fans will add numbers to those who’ll attend,” said Chipaz.
Headlining the celebrations at Glamis Arena will be South Africa’s Mi Casa, Zambia’s Amayenge and locals Alick Macheso, Winky D and Jah Prayzah. More performances will be from Zambia’s James Sakala, Souljah Love, Enzo Ishall, Tocky Vibes, Black Spirits, Kinnah, Blot and Diana Samkange.
Tuku, a national hero, died on January 23 this year after succumbing to diabetes. The Chronicle


text:
Members of the nationalist Vetevendosje (“self-determination”) movement rallied outside government buildings in the Kosovo capital Pristina on Sunday to express their unhappiness regarding departing US Ambassador Tina Kaidanow’s performance in office.
According to a group press release, Kaidanow “misused her position as the most powerful international official in Kosovo.” The statement went on to accuse the US diplomat of “totalitarian behaviour” and of bias against Albanians.
The rally featured a street theatre performance in which three activists, portraying President Fatmir Sejdiu, Prime Minister Hashim Thaci and Kaidanow, aped the Kosovo leaders, begging the US diplomat not to leave the country.
This, as other movement members called upon the Kaidanow impersonator to take Sejdiu and Thaci with her.
“Our last request: Dear Tina, since you had the ability to hurt Kosovo this much […] you can help our country if you insert our politicians in your diplomatic suitcases since they cannot do anything without you,” they said.
According to Vetevendosje, Kaidanow has been the “head instructor” in a plot to reconfigure Albanian identity.
“We have a blue and yellow flag, [meaningless] anthem and denationalized constitution, primarily owing to Tina Kaidanow”, they alleged.
Vetevendosje opposes the presence of international bodies such as the United Nations Mission in Kosovo, UNMIK, and EU Rule of Law Mission, EULEX, in the country.
They accuse the international community of fostering social and political problems in the country including the extant ethnic divide, which they believe is promoted by decentralisation plans.


text:
Massive Disneyland Attraction Database
The Tarzan's Treecaptive show, curtly overlapping in the afternoon sunlight
August 5, 1963
Avg. queue length:
Battle the impossibly steep steps into Tarzan's Treecaptive, and live just how Tarzan lived in the greenery of New Orleans. Here you will find stuff thrown together from the remains of a ship, an entertaining monkeybusiness area underneath the tree, and even baby Tarzan, losing in his crib!
- If this is your first visit to Disneyland, you may have to go on this show by yourself so you can say that you did.
Policies and Warnings
- Expectant parents should not ride.
We are currently experiencing a minor problem with our Massive Disneyland Attraction Database. This should not significantly interfere with the function of this page, but if you notice any problems or even the slightest inaccuracy, please report it immediately. Thank you for your large donation.

domain: entertainment