The Carpet Chemist Rug Cleaning Lab is a 3,000 square foot facility in a small town located between Louisville and Lexington, Kentucky, that was built and designed for maximum rug cleaning and rug dyeing performance. Proper lighting, airflow, temperature, humidity levels, soft water and draining are all essential to giving your rug the most thorough cleaning imaginable. Rugs from all over the globe are shipped to our rug cleaning lab for cleaning, repair, and dyeing. Experts in cleaning silk, wool, Navajo, Turkish, Persian, and Chinese rugs, we have the best solutions for any rug quandary.
Tufted, hand-tufted, hand-knotted, and machine made rugs are not created equal. For instance, a Flokita rug is much different than a synthetic machine-made rug from Costco, and so it should be treated entirely different when it comes to cleaning. You don’t have to worry about asking your carpet cleaning technician if he can clean your $200 rug with his steam cleaning equipment, but a wool or silk rug could potentially be ruined by the typical steam cleaning process. Harsh carpet cleaning chemicals can remove color from your woven treasures, or create wrinkles, browning or even structural damage or dye bleeding. Expensive Oriental and Persian rugs should always be cleaned by rug washing professionals at a facility that is set up to scrupulously wash fine rugs.
The following is a step by step account of our rug cleaning process. Each and every rug has a unique set of circumstances that are managed on an individual basis, but this will provide you with a general idea of how your rugs will be cared for.
As highly trained rug cleaning professionals, we start by inspecting your rug’s fringes, fiber type, pet odors, sttains, moth damage, burns, color-fading, and a host of other variables involved in determining the proper way to clean each rug. For example: natural dyes used in the making of your hand-made rug will not appear to have an even and consistent color, like synthetic dyes typically do. Natural dyes can be made from bugs, berries and other plants. Another very important element to look for is moth damage or larvae. Moths can consume and destroy natural fiber rugs if not eradicated immediately.
“Dusting” or dry soil removal as we rug geeks prefer to call it, is absolutely without a doubt the most important part of the rug cleaning operation. An uneducated carpet cleaner might blast copious amounts of scalding hot water down into your rug and extract maybe 40% of it. Evaporation and time would do the rest. But here’s the problem with that. Dry soil is caked way down into the rug fibers, and there’s no possible way your Dyson is ever going to remove it all. I guarantee it. The Carpet Chemist utilizes special equipment that beats every morsel of food and dirt out of your rug before it ever enters our rug washing area. Without this crucial step in the rug cleaning process, your rug would be destined to have hardened dirt in it that destroys the structural integrity of the rug itself.
In many parts of the world, rug cleaning is done in a river. The cold water flushes out every single bit of material that doesn’t belong. At the Carpet Chemist Rug Cleaning Lab, we embrace the same concept that’s been used by rug washers for thousands of years, except we use our fancy modern equipment and flush our rugs out in a submersion pool, or “artificial river.” This is the only way to make certain that contaminants such as urine are completely and totally eliminated from the rug. The time a rug spends in the submersion pool depends on the amount of urine saturation and other contaminants that are in the rug.
Depending on the fiber type of the rug, we will add the exact amount of special formula needed (wool fiber cleaner, silk fiber cleaner, etc.). Once the solution has been properly and evenly applied, we will agitate the rug with brushes (different brush types for different textile types). This will ensure that each and every fiber is being adequately scrubbed from side to side and top to bottom. This is where those stubborn stains like candy, syrup, and other hard sticky substances become dislodged.
The final step in cleaning the largest portion of the rug is flushing out every bit of rug cleaning solution and dropping the pH level back down to a neutral state by rinsing with a safe low pH rinse. This is what makes the colors in your rug pop and also what makes it feel toe-squishingly soft again! This is a very intricate step in the rug cleaning procedure, and should never ever be rushed.
Fringe cleaning might just be the most important part of all. The fringes are actually the foundation of your rug. There are many types of fringes and many ways to clean and whiten them. The Carpet Chemist has a solution for each and every rug fringe need.
After your rug has been fully cleaned and manicured, we always encourage you to opt for the spill guard fiber protector. It’s true that we are able to remove extremely difficult stains, but by having the spill guard fiber protector applied, spills aren’t able to become stains. Spills will actually bead up on top of the rug fiber instead of being absorbed and staining.
Yes, there is a science to drying. Psychrometry is the science of drying. When drying extremely valuable heirloom rugs, we place them in a special drying room that is temperature controlled with powerful heated air movers and dehumidifiers. We generally allow 1-3 days for rug drying depending on the density of the rug.
Rugs are all very different in fiber type and length, so the Carpet Chemist has a cornucopia of grooming rakes and brushes used to give your rug the very best look possible. Fringe grooming is another form of grooming that give your fringes a neat and clean uniformed appearance.
In Louisville, Kentucky the Carpet Chemist is the professional oriental rug cleaning team to call at (800) 515-1342.
We are rug specialists with the resources and training to care for any oriental rug—from the most delicate heirloom to the boldest designer graphic.
The Carpet Chemist developed the most environmentally safe textile protector that has ever hit the market. We use it specifically on rugs and carpet, but our customers love it so much that per their request, we have applied it to their curtains, dining room chairs, living room upholstery, mattresses, and even boots and clothes. In the event of a spill, you will actually notice the liquid pool up on the tips of the rug fibers without being absorbed by the rug one single bit. It’s something you just have to see in person.
We are so confident in our Rug Fiber Protection program that if you have a stain on your rug within 1 year of having the rug fiber protection applied, we will remove the stain for free. It’s our guarantee to you. So trust your heirloom rugs to The Carpet Chemist and enjoy the peace of mind that comes with world class workmanship and top-quality products.
When your rugs are treated with our Rug Fiber Protection formula, it forms an invisible shield around the rug fibers as it dries. That means microscopic sand will not scratch the rug fibers as you walk over them. Spills will not become sticky messes that attract dirt. It means that your rugs will look as good a year later as they did when you had them cleaned and protected. Every single carpet and rug cleaner and protector that The Carpet Chemist formulates is safe for the environment, people and pets.
The Carpet Chemist says: Protect your heirloom rugs with our Rug Fiber Protection formula. It’s like having insurance for your rug, with a worry-free guarantee!
Some carpet cleaning companies use a blanket approach to every job without considering important factors like the solubility of grime or the required pH of cleaning solutions. The Carpet Chemist is different. We apply science to built-in dirt, stubborn carpet stains and color loss. When you have a problem with your carpet or rug, call the Carpet Chemist at (800) 515-1342. One of our knowledgeable specialists will respond with a free telephone quote and a timely appointment. That’s our commitment to you: amazing workmanship and unbeatable service.
Upload a pic of your rug for an instant rug washing quote. Our IICRC-certified team provides no-obligation estimates. Connect with our team at (800) 515-1342 or by using the secure form below.


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Oxygen transfer, the process by which oxygen is transferred from a gaseous to the liquid phase, is vital in a variety of biological and hydrometallurgical processes, in particular wastewater treatment. The aim for this experiment is to evaluate how this oxygen mass transfer varies under a range of different operational conditions (E.g. Stirrer speed and oxygen flow rate), and formulate the optimal parameters for modelling a sewerage plant.
In wastewater treatment plants, diffused air system bubbles are distributed from diffusers at the base of the reactor, where the rising bubbles come in contact with the aqueous layer (BLA). When equilibrium is reached, the liquid is ‘saturated’ into the gas. This can be modelled using equation 1, as proposed by Merchuk et al(1990), where Cl is the gas concentration in the bulk liquid phase (ppm), t is time (sec), Cs is the saturation level of the gas in the liquid phase (ppm) and K is the overall mass transfer rate coefficient (sec-1). This can be further simplified to yield equation (2)
Both stirrer speed and oxygen flow rate significantly effect the mass transfer rate ox oxygen in a liquid. Altering these two parameters affects the size and flow of the bubbles which impact the surface area in contact with the liquid and hence the mass transfer. Higher stirrer speeds more efficiently break the air bubbles and consequently increase the superficial area of the bubbles, enhancing the oxygen transfer rate(Karimi et al 2013).
In order to measure the dissolved oxygen within each batch condition, Clark-type electrode probes will be used. These sensors are constructed with a silver anode and a gold cathode, which when submerged in an electrolyte (water) complete the electrolytic cell and serve as a reference electrode. The oxygen molecules present in the aqueous solution diffuse through the membrane resulting in a difference in the oxygen pressures. This produces a perceivable current that is recorded by the instrument.
Reference: http://www.ijehse.com/content/10/1/6 Results qualitative effect of stirrer speed
It was observed that with an increase in the stirrer speed(rpm), the K of the system also increased. This can be attributed to…

domain: chemistry


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페이지 정보작성일2003-05-07 Hit7,777
12th 8-Day Husband’s Workshop Postponed
Due to the postponement of the World Culture and Sports Festival, the 12th 8-Day Workshop, which was scheduled for May 26 to June 2nd has been postponed to July.
The exact date will be announced later. Sorry for the inconvenience.
Cheongpyeong Training Center

domain: news


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Benton Harbor teen crowned Mr. Blossomtime
BENTON HARBOR, Ind. (WNDU) - This week, we told you about how Miss Berrien Springs, Faith Kittleson, was crowned 2020 Miss Blossomtime.
Well, we want to introduce you to Mr. Blossomtime, Jeremiah Sterling Jr. of Benton Harbor.
Because of the pandemic, the pageant was delayed six months and was held outdoors under a tent at the Golf Club at Harbor Shores in Benton Harbor.
Congrats to both Kittleson and Sterling on the accomplishment!
Copyright 2020 WNDU. All rights reserved.


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As a responsible cat owner, you want to make sure your furry friend is getting the best nutrition possible. That’s why raw chicken necks are a great addition to their diet. But where can you buy them? Here are some places to check out.
Local Grocery Stores
Many local grocery stores sell raw chicken necks. Just check the meat section for them. However, some stores may keep them in the freezer, so be sure to ask a staff member if you can’t find them.
Some good places to look for raw chicken necks include:
- Whole Foods
- Trader Joe’s
If you have a farmers’ market in your area, that’s another great place to buy raw chicken necks. The necks will be fresh and you’ll likely get them at a lower price than what you’d pay at the grocery store.
Just be sure to ask the farmer how they raised the chickens and if they were given antibiotics or hormones. You want to make sure the necks are coming from healthy chickens.
If you have a local butcher shop, that’s another excellent place to buy raw chicken necks. The quality will be very high since the necks are coming straight from the butcher.
You may pay a bit more than you would at the grocery store, but it’s worth it for the peace of mind knowing you’re getting top-quality chicken necks.
If you can’t find raw chicken necks locally, you can always buy them online. There are a few different online retailers that sell them, such as:
- The Honest Kitchen
- Primal Pets
- Vital Essentials
When buying online, just be sure to read the descriptions carefully. Some retailers sell necks that are meant for dogs, not cats. You want to make sure you’re getting chicken necks that are specifically meant for felines.
Finally, you can also buy raw chicken necks at some pet stores. This is usually a good option if you’re already buying other cat supplies there.
Just be sure to read the labels carefully. Some pet stores sell chicken necks that are meant for dogs or that contain additives like propylene glycol. You want to avoid those and stick with necks that are specifically meant for cats and that are 100% natural.
Raw chicken necks are a great addition to your cat’s diet. They’re packed with nutrients and help keep your cat healthy. Just be sure to buy them from a reputable source so you know they’re of the highest quality.

domain: food


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The impact of the 24-hour ‘Jal Deeksha’ on the Congress High Command was quite evident on Tuesday when Union Law Minister and party In-Charge of AP Affairs Veerappa Moily rushed to Hyderabad and confabulated with Chief Minister N Kiran Kumar Reddy at the Camp office.
The unannounced visit by the High Command’s delegate and his subsequent meeting with Mr Kiran Kumar Reddy has let loose a flurry of activity among journalistic circles. According to sources, Mr Moily discussed the unfolding political scenario in the wake of 23 Congress MLAs and four Legislators from other parties participating in Mr Jaganmohan Reddy’s Jal Deeksha at the Jantar Mantar.
The sources say that the visit also discussed the possible disciplinary action to be taken against the Congress Legislators for violating the High Command’s wishes. The sources further add that the meeting also took into account the possibility of the party Legislators resigning from their seats to press for the bifurcation of the state. The High-Level meeting is believed to have adopted a policy of ‘soft-handed approach’, considering the precarious positing of the ruling party in the state Assembly. The Congress has presently 155 Members among the 294 MLAs in the state Assembly. Added to this, are the 18 PRP MLAs and seven from the MIM. If the 23 Congress MLAs and the two PRP MLAs decide to favour Mr Jaganmohan Reddy, the Congress will be in a precarious position, since the MLAs from Telangana have already threatened to resign.
Mr Jaganmohan Reddy had issued a veiled threat stating that if, he wants, he can pull down the government but would not do so. The crucial meeting of Congress leaders from Telangana currently under way at Kalinga Bhavan in Banjara Hills also came up for discussions during the meeting, according to sources.

domain: politics


Fujikura Ltd and NTT Docomo Inc jointly started a test to apply a fuel cell system (rated power: 1kW) using a direct methanol fuel cell (DMFC) method being developed by Fujikura to NTT Docomo's "green base station."
Green base station is a facility equipped with solar panels. It can be independently operated at the time of disaster without power from a power grid. By operating the DMFC in conjunction with the solar power generation system, the base station can independently operate for more than a week.
The DMFC directly converts the chemical reaction between methanol and oxygen (in the air) into electricity, realizing high-efficiency power generation. It does not generate toxic substances and is very quiet. Its rated output and voltage are 1.0kW and DC48V, respectively. The size of the system is 137L (460 x 460 x 650mm), which is 1/20 the size of conventional large-size stationary fuel cell systems.
The DMFC features a high portability. It can be installed for small base stations located in narrow spaces, on the roofs of buildings, etc and carried to a disaster-hit area in an emergency. When applied to a base station, it can be operated for four days (if there are five units and 200L of fuel).
In the joint test, the DMFC is operated for long hours in consideration of a disaster at a green base station that NTT Docomo operates in Nankoku City, Kochi Prefecture. The solar power generation system and the fuel cell are operated in conjunction with each other in the aim of testing the effectiveness and reliability of the fuel cell as a measure against disasters. The test will end Sept 30, 2018.
As part of their efforts for safety and security, Fujikura and NTT Docomo aim to commercialize an environmentally-friendly system in the 2020s through (1) the development of compact, eco-friendly fuel cell systems that can be used for powering base stations and charging terminals in case of emergency and (2) a research for producing fuel (e.g. methanol) for the system from renewable energy.


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Leah Windt and Jenny Lawton brought home first places for the Whitnall girls track and field team to key the Falcons' efforts in the Cedarburg Invitational on March 19.
Windt won the 800-meter run in 2 minutes, 34.22 seconds and Lawton took the 55 hurdles with 10.58 seconds for Whitnall, which tallied 58 points for fifth place in the meet.
Coming in third for the Falcons were Jenny Bahling in the 800 run (2:40.96) and Jasmine Van Tine in the 1,600 run (6:07.47).
Taking fourth were Brooke Berglund in the 3,200 run (13:35) and Hannah Fischer in the 55 hurdles (10.72). Claiming fifth were Alyssa Woo in the 3,200 (13:38) and the 640 relay (1:39.81).
Lani Cieszynski finished sixth in the pole vault (6 feet, 6 inches) and Kim Hurst seventh in the 1,600 run (6:21.9).
Coming back with eighths were Amanda Rosenwald in the 400 dash (1:12.79), Sarah Czerwinski in the 55 dash (8.8) and Kina Cross in the 1,600 run (6:31.33).
- John Rech
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- Reed, Bickerstaff claim seconds for Whitnall at Woodland Conference Outdoor meet
- Greendale boys golf team brings home Woodland Conference crown
- Whitnall boys tennis team takes home pair of titles from Woodland tournament
- Greenfield's Nguyen, Kohl win events at Woodland Conference Outdoor meet
- Whitnall's Reed earns three top-four finishes at Woodland Conference Outdoor meet
- Germantown girls track team smashes records, set for NSC
- Greenfield's Larry Sayles' decision to become athletic trainer opened up his world
- Greenfield softball team clinches Woodland Conference East Division crown
- Whitnall softball team shows resiliency while battling injuries
- Nguyen leads Greenfield girls track and field team at Homestead Invitational

domain: sports


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About 100 NY-based tech startups creating consumer health and wellness products have launched recently. But these entrepreneurs face bigger challenges than those specializing in social media.
According to Crain’s New York Business, in this field it’s hard to make money just by targeting consumers. They have to include hospitals and health insurers, which often means providing documented proof that a product or service meets approved standards. An example given is the founders of WellApps, who initially offered their product, which tracks the symptoms of Crohn’s disease, for free. When they wanted to sell the app through insurers, they had to provide scientific proof the app improves patient health. Now WellApps is sponsoring a clinical trial.
Founders also need to understand complex regulatory requirements as well as a familiarity with the bureaucratic nature of hospitals, insurance companies and other healthcare organizations, as Crain’s mentions two new venture accelerators have stepped in to help. Startup Health plans on providing mentorship and funding to about 1,000 healthcare entrepreneurs during the next 10 years, while BluePrint Health just announced its first incoming class of nine startups.


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I saw this on Miss Moss yesterday (apologies if you did, too!) and had to give it its own post rather than saving it for an Elsewhere link. The Third Hand is a collection of pieces by a young Chinese designer (a Central Saint Martins grad) named Momo Wang, entirely upcycled from second-hand clothing. The Chinese textiles are amazing, of course, but mostly there are lots of idiosyncratic pullovers, headpieces and kimonos fashioned from old sweaters that have been patchworked, embellished or unraveled entirely. Don’t miss the turban wound of still-kinky wool, with a pompom at the forehead. The photos by Shuwei Liu are so beautiful; same with the video.
My oft-mentioned pal Leigh is a master of this sort of thing — sewing leather bags out of old jackets and so on — albeit at the opposite end of the aesthetic spectrum from young Momo. You should see the elegant black ribbed cowl she knit from the yarn of a cashmere sweater whose sleeves were worn out.
Between them, I’m inspired to do some upcycling.
Love upcycling. It’s such a challenge and so much fun. These are amazing!
They’re not my look, obviously, but I’m inspired by the inventiveness. I could stand to be more inventive.
Oh, I don’t know Karen, you are pretty darn inventive!
Wow. Love the photos. Especially Girl With Rooster.
I was suggesting the other day that a friend make leg warmers from the sleeves of thrift-store sweaters.
Portrait with rooster is my favorite photo of the bunch.
Could get lost for days in those images. I find myself attracted to knit pieces that combine some sort of leather embellishment.

domain: artistic


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Volunteer With 'Deep Knowledge' Of Chatham History Remembered
CHATHAM – The two things you could always count on from Ben Nickerson were a smile and a kind word.
“Ben always left you with a smile on your face,” said Jean Williams, who runs the FISH medical transportation service, for which Mr. Nickerson volunteered. “He was a people person, with all the best qualities, upbeat and had fun stories to tell and never a negative word about anybody else.”
An 11thgeneration descendant of Chatham founder William Nickerson, Mr. Nickerson passed away last Thursday, Jan. 24. He was 84 years old.
Benjamin T. Nickerson was remembered for his deep knowledge of Chatham history and people, his service as a volunteer firefighter, town official and Mason and his devotion to the Nickerson Family Association. Many in town knew him through the business he and his wife Marilyn started in 1970, Benjamin T. Nickerson Inc., a rubbish removal service now operated by his son Benjamin and daughter Pamela. He could often be seen driving the company's big green truck smiling and waving to people along his route.
Ralph Crowell met Mr. Nickerson in 1949 when he moved to town after getting married to a local woman. Both served on the town's fire department and were past masters at St. Martin's Masonic Lodge.
“He always looked on the positive side,” Crowell said of Mr. Nickerson. “He made the best of every situation that came along. He was very positive, that's what made everybody like him.”
Mr. Nickerson's father, Benjamin L. Nickerson, was on the town's police department, and Mr. Nickerson grew up in Chathamport steeped in the local traditions and stories of his ancestors. “He was a Cape Cod Nickerson, without a doubt,” said Crowell, also a Nickerson descendant.
Mr. Nickerson served in the Coast Guard, meeting his wife, Marilyn Strutzel, while stationed in Alaska. After living for three years in Aurora, Minn, his wife's hometown, the couple returned to Chatham where Mr. Nickerson followed in his father's footsteps, serving as a police officer and firefighter. He also continued to serve as a Coast Guard reservist, and played a role in what was perhaps Chatham's most famous incident in the 1950s, the rescue of the 32 crew members from stern section of the tanker the SS Pendleton.
On Feb. 18, 1952, he was attending Chatham's annual town meeting at the Chatham Theater – now the Chatham Orpheum – when Moderator Igo Toabe made an announcement about the rescue and asked that emergency personnel and anyone with a station wagon head to the fish pier to help transport the frigid and shaken crew. Mr. Nickerson left the meeting and borrowed his neighbor's 1947 Ford Woody, drove to the pier and took Pendleton survivors to the Coast Guard Station.
“Everybody was cold and all bundled up and there wasn't much conversation going on,” he recalled in a 2016 interview. He said he always looked up to Coxswain Bernie Webber, who piloted the CG36500 against overwhelming odds to rescue the Pendleton crew.
“He was like a star to me to even attempt that,” Mr. Nickerson said.
For 28 years Mr. Nickerson was a volunteer for the Nickerson Family Association, in recent years volunteering on genealogical projects every Wednesday morning. He was a mainstay of the annual Nickerson Family Reunion, and was relied upon for his long memory of Chatham's people and history, said Nickerson Family Association Executive Director Debra Lawless.
“What impressed me the most was how if I asked him a casual question about someone in Chatham, he'd give me the person's life story. And it was all accurate,” said Lawless.
He had incredible institutional knowledge about the family and the town, said Ron Nickerson, a past president of the NFA. “It's a real loss to the organization,” he said. “We don't have anybody to ask anymore.”
Mr. Nickerson was instrumental in the Nickerson Family Association's effort to save the 1829 Caleb Nickerson house in 1997, serving as head of the project's finance committee. He helped oversee the relocation of the antique Cape via barge from Nickerson Neck to Ryder's Cove. It was moved just down Route 28 to the Association's property where it now serves as a museum. At the time the location was thought to be near William Nickerson's original homestead; recent archaeological digs confirmed the existence of a house from that era, probably William Nickerson's, on land just behind the Nickerson Family Association property.
“He was a fantastic family member and volunteered doing anything they required, with a big smile on his face,” said Phyllis Nickerson Power, who worked with Mr. Nickerson on NFA projects. For the Association's float in the Fourth of July parade in 2012, Chatham's 300thanniversary, Mr. Nickerson donned a period costume and took on the role of his famous ancestor, William Nickerson, with Power playing the role of William's wife Anne Busby. It was a hot day and she'd brought along a water pistol, soaking people in the crowd even though it was against parade rules.
“He laughed!” Power recalled. “He just had the best grin and the best laugh. He will be so sorely missed, just because he was a ray of sunshine all the time.”
Mr. Nickerson was a popular driver for the FISH transportation service, which provides free rides to medical appointments. Many clients requested him as driver, and he had to be put on limited duty because he was wearing himself out by driving so much, Williams said.
“He felt it gave him the opportunity to meet people he otherwise wouldn't meet,” she said of his work with the organization. “He was absolutely one of the best [drivers] we ever had. He cared about other people.”
Mr. Nickerson grew up in Chathamport and attended school – all 12 years – in the former Main Street School, now the town's community center. He built a home in West Chatham in 1960 where the couple raised their four children, Linda, Pamela, Kim and Benjamin. He worked as a mechanic, bus driver and for his brother-in-law's trash hauling business before starting the rubbish removal company, according to the Nickerson Disposal website. He retired in 2009.
Along with serving as a police officer and volunteer firefighter, Mr. Nickerson was an elected constable and served on the recreation commission for nine years. He was a long-time member of the First Congregational Church of Chatham, and spent many hours volunteering with the Masons.
“He was a great, great guy,” said Crowell. “He was an asset to the town of Chatham, without any question about it.”
At Mr. Nickerson's request, there will be no service. The family plans a celebration of his life in the spring.


Zaha Hadid, a pioneering architect who became the first woman to win the prestigious Pritzker Architecture Prize in 2004, has died at the age of 65 following a heart attack in Miami.
Born in Baghdad, Iraq, Hadid was educated in London and later became a naturalized resident of the United Kingdom. In recent years, she had become a part-time Miami resident. According to reports, she had developed bronchitis earlier this week and was admitted to a local hospital. She suffered a surprise fatal heart attack overnight.
“It is with great sadness that Zaha Hadid Architects have confirmed that Dame Zaha Hadid, DBE, died suddenly in Miami in the early hours of this morning," read a statement from her architecture firm.
Hadid had only recently brought her talents to Miami. She designed the One Thousand Museum building, a towering 60-story condo skyscraper with a distinct exoskeleton structure, which is under construction downtown. She had also drawn up plans for a sprawling, futuristic public parking garage in Miami Beach's Collins Park neighborhood, but the project has stalled due to budget concerns.
Her installation, "Elastika," is also prominently displayed in the atrium of the Moore Building in the Design District, which is currently the temporary home of the Institute of Contemporary Art Miami.
She frequently reminded many that cut her commission rate at One Thousand Museum just for the chance to add to Miami's skyline.
"We're charging not very much," she once said of the project. "It's a present from me."
She spent many holidays in the city and owned an apartment at the W in South Beach.
"I go there and I just chill out," she once told the Wall Street Journal.
And she wasn't afraid to criticize the city's existing architecture. Notably, she disliked the design of the under-construction Frost Science Museum, which sits just across from the One Thousand Museum site.
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"I really love Miami, but I don't think the architecture matches the city," she told the paper. "It's a bit too commercial."
One Thousand Museum is scheduled for completion in 2017. It will be one of the Hadid projects in North
Star architects taking their talents to South Florida has become common over the latest development boon. Fellow Pritzker Prize winners Rem Koolhaas, Frank Gehry, Norman Foster, Herzog & de Meuron, and Jean Nouvel all have projects planned, under construction, or recently completed in Miami. Perhaps none has brought as much buzz as Hadid.
She was at the top of the game, always innovative, and left the architecture world eagerly anticipating her next move. A blunt talker with a flair for fashion (word is she was one of the most frequent customers at the Design District's Maison Margiela boutique), she cut an imposing figure and was as famous a figure as an architect could be.


Our Workshop: We Repair, Service & Rebuild Any Wood Chipper!
Our workshop has been a hive of activity in the last few weeks! We are busy with quite a few rebuilds, services and seasonal maintenance on units to get them ready for the pruning and chipping season. In the last 6 weeks we have serviced and rebuilt more than 10 wood chippers of various makes. No matter what the brand and the size of the unit is, we can service them all.
We can do the following on any type/size wood chipper:
– restore your wood chipper to its original condition
– repair/fit/replace any worn parts on the structure of the unit
– service diesel & petrol engines and PTO-driven units
– hydraulic repairs & service of hydraulic systems
– drum & disc repairs on the blade pockets
– in-house drum & disc balancing
– respray units in any colour as requested by the customer,
– fit the unit with safety stickers and logos too
Contact us today for a quotation. We collect the unit at your premises/farm and will deliver it again. We provide a full warranty on all our repairs.


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Published: June 26, 2020
The Hand Hygiene for All initiative aims to implement WHO’s global recommendations on hand hygiene to prevent and control the COVID-19 pandemic and work to ensure lasting infrastructure and behaviors. This document outlines why universal hand hygiene is important and how multiple actors can work […]
Published: May 4, 2020
COVID-19 is a reminder of the critical role of hand hygiene in preventing diseases and improving health outcomes. As the first hands to touch a newborn baby, midwives protect the lives of mothers, their children, and their communities. In Lagos, Nigeria, a team of midwives […]
Published: April 20, 2020
These messages developed by Sanitation and Water for all can be used when advocating for WASH in response to COVID-19. These messages are available in English, Spanish, and French here.
Published: April 16, 2020
This poster was developed with the support of the Kenyan National Business Compact on Coronavirus. The poster highlights preventative measures, sign and symptoms, and ways to protect yourself and others from coronavirus. This poster is available in English and Swahili.
Published: April 16, 2020
This Frequently Asked Questions document is based on a December 2019 webinar on the local production of alcohol-based hand rub in Uganda for healthcare workers by Infectious Disease Institute at Makerere University and the US CDC.
Published: April 1, 2020
This document developed by WHO, UNICEF, and IFRC provides key messages and actions for COVID-19 prevention and control in schools.
Published: March 23, 2020
Coronavirus disease 2019 (COVID-19) is a respiratory illness that is caused by a novel coronavirus first identified in an outbreak in Wuhan, China. The disease can spread from person to person contact, both through direct contact with an infected person or through contact with surfaces […]
Published: March 16, 2020
The 2020-2024 Global Handwashing Partnership strategic plan emphasizes the GHP’s focus on advocacy and knowledge management, and highlights the greater need for integration and country-level action. In addition, the plan showcases the GHP’s new governance and financing structure.
Published: March 5, 2020
This Technical Brief supplements existing IPC documents by referring to and summarizing WHO guidance on water, sanitation and health care waste which is relevant for viruses, including coronaviruses. WHO has also published a number of technical guidance documents on specific topics, including infection prevention and […]
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© 2017 The Global Handwashing Partnership (GHP).

domain: medical


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1) Season cut up chicken with salt and pepper. When coals are white, spread them out for even heat distribution. Place chicken pieces on the griddle with larger, fattier pieces (such as the thigh) near the centre and smaller pieces to the outside.
2) Brush the griddling sauce on chicken through the cooking process and turn chicken as needed. Move chicken toward the outside as the leaner pieces (such as the breast) get done. Brush with griddling sauce just prior to removing from griddle.


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Michigan Law Enforcement Leaders: Invest in Early Education or Foot Bigger Prison Bill Later
LANSING, MI (September 9, 2009) — At a news conference today, law enforcement leaders with the crime-prevention nonprofit organization, Fight Crime: Invest in Kids Michigan, released a research brief entitled, “Invest in Early Education Now, Spend Less on Prison Later.” The research indicates that high-quality early learning programs for at-risk children ages birth to five can significantly reduce crime and ultimately cut corrections costs by a quarter or more.
Currently in Michigan, there are more than 79,000 incarcerated adults in jails and state prisons, with corrections costs exceeding $2 billion every year. The crime fighters said that Michigan would save about $500 million in taxpayer dollars if it cut prison costs by a quarter by investing in early learning.
With kids heading back to school, law enforcement leaders are calling upon the Governor and State Legislature to keep funding for early childhood care and education programs that have been slated for cuts or elimination, as some of the best investments to save taxpayer dollars and to curb future crime. They are also urging Senators Levin and Stabenow, as well as the rest of the Michigan Congressional Delegation, to support new federal legislation to implement the proposed Early Learning Challenge Fund, which will provide $1 billion per year for states to expand and improve their early childhood development initiatives.
Law enforcement leaders said it would be short-sighted for state lawmakers to eliminate more than $200 million in state funding for 30,000 preschool slots, child abuse and neglect prevention services to more than 14,000 children ages zero to three, and reduced child day care services for children of the working poor, when these are the proven investments that can reduce prison spending.
“We’re here to talk about dollars and common sense,” said Mt. Morris Township Chief (retired) Eric King, state co-chairman of Fight Crime: Invest in Kids Michigan. We know how expensive it is when someone becomes a career criminal. If getting these kids access to early learning can help us avoid those enormous costs, then we need to make sure they have that opportunity today.”
A long-term study of the high-quality Perry Preschool in Michigan found that by age 40, the kids left out of the program were 85 percent more likely to be sentenced to jail or prison. Another study detailed in the report showed that at-risk kids who did not attend Chicago’s Child-Parent Centers were 24 percent more likely to be incarcerated than similar kids who did attend.
Battle Creek Police Chief David Headings emphasized the cost-saving benefits of investing in high-quality early childhood education and care, especially for at-risk young children. Researchers at the Federal Reserve found that the Perry Preschool program had an annual rate of return on investment of 16 percent. The majority of the cost savings in the Perry study came from reductions in crime and incarceration.
“If there is one thing I’ve learned from my 32 years as a cop, it’s this: you pay now or you pay more later. You can pay now by investing in early care and education for our youngest kids, or you pay much more later through unsafe communities and prison costs,” Chief Headings said. Headings added, “The consequences of the June 2009 State Senate vote that now threatens to eliminate 30,000 preschool slots will continue to result in increased prison costs.”
A new federal initiative, the Early Learning Challenge Fund, will support early education programs, such as Head Start, Early Head Start, pre-kindergarten or quality child care, which offer constructive environments for the healthy growth and development of young children in the first five years of life. The education committee in the U.S. House of Representatives passed a bill in July that includes support for the Early Learning Challenge Fund.
Lapeer County Prosecutor Byron Konschuh said that more support is needed at the state and federal level to ensure that quality early childhood programs are available and affordable to more families. Costs for enrolling young children in early learning programs can run as high as $7,300 a year, which many families are unable to afford.
“Our escalating prison costs are the price we pay for not making these early learning investments years ago,” Prosecutor Konshuh said. “We can arrest, prosecute, and lock up criminals, but there is no substitute for getting in front of the problem by investing in the care and education of our youngest children.”
The need to increase access to high-quality early learning opportunities is great. For example, the federally-funded Head Start program for children in poverty serves only half of eligible children nationwide due to inadequate funding. And the youngest children, from birth to age 3, are even more dramatically underserved. In fact, Early Head Start serves only about three percent of eligible infants and toddlers nationally.
The research shows that quality is essential to getting the crime and incarceration reduction benefits of early learning. The Early Learning Challenge Fund legislation will enable states to adopt best practices, including higher qualifications for teachers and caregivers. In addition, it encourages smaller class sizes, and early screening and treatment of mental, emotional and behavioral problems, as well as parent coaching, which teaches at-risk families ways to promote their children’s development.
“Law enforcement needs all the help we can get. Today, with dwindling state dollars, we need to take advantage of all the federal partnership opportunities available to save kids and reduce crime,” Milton Scales, Chief of Criminal Investigations for the Michigan Department of Environmental Quality, said. Scales added, “The Early Learning Challenge Fund can help Michigan children by closing the gap in services needed and programs provided. To protect the public safety we need to be as willing to provide children a slot in a preschool class as we are to guarantee them a prison cell.”
An electronic version of the research brief may be obtained at: fightcrime.org/reports/miprek0909.pdf
Eric King, Byron Konshuh, David Headings, and Milton Scales are Michigan state executive committee members of FIGHT CRIME: INVEST IN KIDS Michigan, an anti-crime organization led by 443 police chiefs, sheriffs, prosecutors and violence survivors, including 5,000 nationwide.

domain: sociology


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Living in the concrete jungle of London, we think the vibe of the vivacious South Americas is a welcome addition to everyone’s lives. Where better to start than their delicious cuisine? South American cuisine is all about Churrasco (grilled meats), succulent steaks, black bean stews and sensational empanadas. Not forgetting their love for finely mixed cocktails, because finding a fantastic Caipirinha in London is something everyone needs to do. Whilst South American cuisine hasn’t gained the widespread attention like other cuisines just yet, we think it is coming up in a big way! So, with this list of our 10 favourite spots, when going out for a Brazilian becomes the new Italian, you’ll know all the best places to go.
Brazilian mealtimes are characterised by the - seemingly mutually exclusive - relaxing and vibrant atmosphere, which is something Cabana in Brixton are fully invested in. You can also count on barbecue grilled meats, succulent vegetables and exciting flavours to be dished up onto your plate. Not forgetting the Caipirinhas! Or the 20% off you can get with your South London Club Card.
Baron, West Norwood
Okay, so this might seem like a bit of a curveball but stick with us. Baron is a Polish and Brazilian fusion delicatessen. If you need authentic meats and cheeses to replicate some South American (or Polish) flavours in your own home, definitely head down there and pick up some fine cuts and produce. It’s not all meats and cheeses either as they offer fruits, vegetables, rice and beans. With 10% off purchases over £10 with your South London Club card, you’ll want to stock up for the week.
With branches in both Croydon and Brixton, Carioca really are bringing authentic Brazilian street food to London in a big way. Not just a BBQ house, Carioca like to infuse South American flavours with other world cuisines to keep their customers coming back for more. Carioca are also about brilliant service, offering a friendly Latino smile to all customers and even catering for vegetarians and Vegans, almost a rarity for Brazilian restaurants! Head down there from Tuesday – Thursday with your South London Club card and you can get 15% off everything.
Senzala Creperie, Brixton
Now for some sweet treats, because even though the savoury meat dishes are fantastic, Brazilian desserts definitely give them a run for their money. Although you may associate creperies with French cuisine, Senzala Creperie are all about bringing a Brazilian flair to this tradition. Offering a huge variety of sweet and savoury crepes, the one thing that doesn’t change is the freshness and quality. With 10% off, South London Club cardholders can feel even better about indulging.
Rodizio Rico, Greenwich
Rodizio Rico do things a little differently to your typical London restaurant, whereupon you pay a set price at the door which gives you access to their salad cart of authentic street food and cuts of their delicious meats. Now this is where it gets interesting. Waiters roam the restaurant with skewers of meat to cut right onto your plate throughout your time at Rodizio Rico. You are given a card with a green and a red side, the green welcoming the waiters to load your plate up with freshly-cooked meats, the red meaning you’re taking a break or you’re full. Maybe you peaked with the buffet. Either way, you’ve got to try out this exciting and social way of dining, right in the heart of Greenwich!
Santa Maria del Sur, Battersea
Argentinian steaks just go one above the traditional British fare you have come to expect and there is no better place to test them out than at Santa Maria del Sur, where they are all about fresh ingredients and colourful plates. Think steaks cooked to perfection, enjoyed in a relaxed atmosphere. We’d recommend going all in here, ordering all three courses and settling in for a feast! Something else Argentine cuisine is big on is delicious red wine, which Santa Maria del Sur don’t do by halves.
El Rancho De Lalo, Brixton
You may not have tried Colombian cuisine before, but we don’t think you need to hesitate any longer. El Rancho De Lalo have established themselves in Brixton as an authority on delicious Colombian flavours. Turn up hungry, because El Rancho De Lalo are all about generous portions, cooked fresh to order. If you’re a meat lover who wants to jump right into Colombian cuisine, we’d recommend the traditional Baneja Paisa.
Located in Bermondsey, Constancia is an Argentine grill and steak house that boast the best empanadas in London. Their beef is cooked in an authentic charcoal parrilla, which they claim is their secret to tender steak that is the ‘best you’ll try’. They source many of their ingredients locally, from Borough Market, and serve a menu that is refined to only the best Argentine flavours. Constancia also pride themselves on their extensive Argentinian wine list, for those who want to wash down their decadent meal with a beverage to match.
Fat Ox, West Norwood
Fat Ox is a butcher specialising in traditional Brazilian cuts of beef, pork and chicken. Attempting to bring a taste of Brazil to West Norwood, they don’t just stop at meats. You can grab some other Brazilian specialties such as churros, acai and rosquinhas. You don’t really need to know what that means, you just need to taste them, trust us. What’s more, South London Club cardholders can get 5% off.
Cabrera Steakhouse, Lee
If you want delicious Argentine steaks and mouth-watering churros served up in an elegant and modern interior, Cabrera Steakhouse is your new go-to. It’s not only their meats that are cooked to perfection, the atmosphere here is wonderful and oft praised, as well as the friendly staff. We’d also recommend their triple cooked chunky chips on the side. A strange choice in an Argentine restaurant, but just trust us on this one.
WHAT IS THE SOUTH LONDON CLUB CARD?
The South London Club is a local discount card to help support small independent businesses across South London whilst saving all who live, work & play in South London money! With over 500+ local discounts to choose from, you will discover & explore all the best hidden gems in South London. Join over 5,000 of us & celebrate all that's independent & South London!


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Yellow Corn Apply More Nitrogen?
It was a couple weeks ago almost every corn field I saw looked about as perfect as it could. Then a little over a week ago parts of most corn fields had areas with corn turning yellow. That is a classic symptom of nitrogen deficiency. Does that mean the nitrogen we applied last fall or early this spring was lost? Jeff Vetch Scientist at the Southern Research and Outreach Center at Waseca said the nitrogen was likely just unavailable until we saw the warmer weather.
Temperatures during April and May were much cooler than normal. Microbes in warm soil convert the anhydrous ammonia which is not water soluble into nitrate nitrogen which is. That is the form that is available to the corn plant. In addition nitrogen that was available in the soluble form may have been in a band deeper in the soil than the corn roots.
Compounding the nitrogen situation was the growth stage of the corn. When corn has two to 4 leaf collars it transitions from nutrients coming from the seed to the developing nodal roots. That too limited the uptake of available nitrogen in the soil as that is the stage that much of the yellow corn was at last weekend when I took this picture. Fortunately with all the heat late last week and over the weekend the yellow corn is looking much better.
Talking with Jeff Vetch Scientist at the Southern Research and Outreach Center at Waseca he said corn with yellow stripes on the leaf is a symptom of sulfur deficiency which he has seen this spring too. Click on the link and listen to Jeff's comments on yellow corn.

domain: biology


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Officer shoots robbery suspect in Southeast D.C.
A Metropolitan Police Department officer shot a 31-year-old man after the man pointed a gun at him after attempting to rob three people.
MPD Spokesman Officer Araz Alali says three men were sitting in a car at Martin Luther King, Jr. Avenue and Sumner Road when they were asked for a ride by a man they knew from their neighborhood. They took the suspect, identified by police as Paul Smith, to the unit block of Chesapeake Street, SE after he offered to give them money for gas. Once they arrived, the suspect pulled out a gun and demanded money from the victims.
Investigators say while he was robbing the men, he noticed one of them had an ATM card and ordered they take him to a bank in the 4100 block of South Capitol Street, SE to get more money.
Alali says an officer patrolling the area saw the suspect pointing a gun at one of the victims at the ATM and ordered the man to drop his gun. The suspect did not comply and instead fled the scene.
The officer followed, and in the unit block of Chesapeake Street, SE, the suspect pointed his weapon at the officer, Alali explained. The officer fired his weapon, striking the suspect multiple times.
Alali says Smith was transported to an area hospital, where he remains in unknown condition.
Smith of Southeast is charged with kidnapping while armed, robbery while armed and assault on a police officer while armed.

domain: law


Mapex Saturn - Sonny G.
(Uvalde, TX., USA)
I just purchased my very first Mapex Saturn Series, 6-piece drum kit. After 30 years of playing Ludwig drums, and more recently, Tama and Sonor kits. I can now say, "This Mapex kit kicks butt and is second to none of these aforementioned kits. Pretty good company, I'd say!"
The sales people where I purchased my drumset were honest enough to tell me they've all played higher-end kits, and they really were impressed with the punch the Mapex Saturn Series delivers.
They could have made more money off me by pushing a more expensive kit, which I initially intended to purchase. Instead, they let me know I wasn't losing sound quality by buying the Saturn Series kit.
The money I saved by choosing the Mapex Saturn Series kit allowed me to purchase quality hardware, cymbals and cases to compliment and protect my new, beautiful Mapex drum kit.
A very happy and proud Mapex drum kit owner.


An evolving workplace, greater reliance on IoT and the cloud, and already we have seen the new face of supply chain attacks. This is the backdrop for 2021, and Imperva's Brian Anderson offers insights into the cyber-attack outlook.
The Zero Trust security model created by Forrester has become a mainstay of security approaches. But traditional Zero Trust controls largely overlook unmanaged and IoT devices, leaving the enterprise at risk.
Read this white paper and learn:
How existing Zero Trust security tools are not designed for unmanaged...
2020 was the year of mass migration to multi-cloud environments, which paves the way for 2021 and a further explosion on microservices and severless cloud computing. Peter Klimek of Imperva discusses how cybercriminals are likely to respond - and how to anticipate them.
A hacking group behind an Android spyware variant has recently added fresh capabilities that include the ability to snoop on private chats on Skype, Instagram and WhatsApp, according to ReversingLabs. This APT group, believed to be tied to Iran, has recently been sanctioned by the U.S. Treasury Department.
You’re securing more endpoints than ever across the largest attack surface ever. Enter: IoT devices and the risks they bring. But they also bring new business benefits. Mitchell Bezzina of Palo Alto Networks explores risks, benefits and new steps to ensure IoT security.
In this one-pager Bezzina...
President Donald Trump on Friday signed into law the Internet of Things Cybersecurity Improvement Act of 2020, the first U.S. federal law addressing IoT security. The act requires federal agencies to only procure devices that meet minimum cybersecurity standards.
A critical component within millions of consumer and enterprise IoT devices has dangerous software flaws. New research from Forescout Technologies into open-source TCP-IP stacks shows millions of devices from 150 vendors are likely vulnerable.
In this eBook learn how organizations can achieve cyber resilience in an increasingly digitized world. Gain quick tips on how to get the board’s attention and approval on CyberSecurity investments. And finally, deep dive into how to combat cyberattacks effectively with a CyberSecurity Incident Response Program.
Twenty-five countries are likely using spyware sold by a company called Circles that can snoop on mobile phone calls and text messages, according to The Citizen Lab, a research organization based at the University of Toronto.
Two vulnerabilities in Tesla's keyless entry system allowed researchers to clone a key fob and drive away with a Model X. The electric vehicle manufacturer is issuing over-the-air updates to fix the flaws, which allegedly center on a failure to validate firmware updates and a faulty Bluetooth pairing protocol.
IoT devices and applications often use a range of components, including third-party libraries and open source code. Steve Springett, who created Dependency-Track, explains how to reduce risk and keep third-party code up to date.
The Telecommunications Security Bill introduced by the British government aims to set enforceable, minimum security standards for the nation's telecommunications providers, backed by penalties, including for any company that opted to use equipment from high-risk providers such as China's Huawei.
This report unlocks visibility in your converged OT and IoT networks for accelerated security and digital transformation. Guardian delivers network visualization, asset inventory, vulnerability assessment, and threat detection in a single application. Download report to know more.
The first six months of 2020 saw an increase in threats to OT and IoT networks, especially IoT botnet, ransomware and COVID-19-themed attacks. These attack types align with global computing and socio-economic trends. The rapid rise in IoT devices and connections, the worldwide COVID-19 pandemic, and the increasing...


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We all have favourite books that we keep and wouldn't think of giving away. I've heard them referred to as 'keepers' when speaking about fiction, but not relating to non-fiction.
I certainly have a few books that I wouldn't part with, mainly because of the subject matter, but they can sometimes cost quite a bit if there isn't a modern paperback reprint available, and not always available from local libraries.
Fashions in Hair: The First Five Thousand Years by Richard Corson.
My hardback copy- slightly tatty- is a 1984 reprint and was bought from a book sale at our County library about eight years ago for 80p. The most interesting 80 pence I have ever spent.
(It is a rather heavy book and 6cm thick.)
It starts with 1400BC and ends with 1978, over 3,000 black and white illustrations- and yes there is a hairstyle shown that I did actually have myself, around 1978. :)
There's explanatory text discussing how the styles developed during specific time periods, with additional images such as 14thC Venetian hairpins to caricatures of hairstyles.
There was a later reprint, but second hand prices are similar to the previous version.
I've not yet found another book that is as comprehensive as this one.
So do you have a reference book that you wouldn't part with ?
Please use the comment form to tell us about it ...

domain: library


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The capital city
Glitz, glamour, golf and more
Center of the Blues Universe
From primitive camping to full-service RV accommodations to cozy cabins, Mississippi's State Parks are fantastic places to stay. For complete information, click here.
Providence Hill Farm and Providence Plantation are available for sporting clay shoots, wedding receptions, corporate retreats, graduation celebrations and other special events.
8-mile round trip partially looped trail nationally designated recreation trail begins and ends at Plum Point Recreation Area in George Payne Cossar State Park; winds through recreation area, sandy edge of reservoir, past deep gullies; primitive campground, swimming beach at Plum Point, large parking area, restroom at trailhead; no hitching racks.
23-mile marked trails in national forest, variety of scenic terrain, native plant life, crosses several local roads, offers option of several trails of varying lengths, 1.5 to 23 miles, open year-round, also primitive camping, nature trails, picnic areas
Trails: 35 miles of back country trails offers hiking, ATV riding, mountain biking and horseback riding.
Recreation: Along with our trails we offer 565-acre lake, 1/2 for fishing & skiing, 1/2 for fishing only; boating, wildlife viewing.
Camping: This campground is located minutes away from Tupelo National Battlefield and Brice's Crossroads. 10 cabins, 10 tent sites, 52 RV sites (paved), 3 pull-thus (60'), 49 back-ins (40'), 52 full hook-ups, 30/50 amps, dump station, slide-out accommodations, pet restrictions.
Facilities: Restrooms, showers, firewood, satellite TV.
15- Mile loop over beautiful terrain in Tombigbee National Forest, shorter loops available; topography formed by Ripley Formation and Prairie Bluff Chalk from Upper Cretaceous Period, approximately 70 million years ago; marine fossils, including shark teeth, fish vertebrae, large extinct clam Exogyra popular. Includes a 23-mile multi-loop scenic horseback riding trail.
THE OFFICIAL TOURISM RESOURCE FOR THE STATE OF MISSISSIPPI

domain: travel


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Nowadays, protein engineering relies on direct or indirect screening methods to sort potentially improved enzymes from all the mutants of a bank. Among these methods, the most accurate performs direct sensing and quantification of the compound of interest, but they remain expensive and require large devices such as High Performance Liquid Chromatography (HPLC), immunoassays or Mass Spectrometry. Devices that not every lab can afford since you need not only the resources to buy the devices, but also highly-skilled collaborators.
To improve the screening process of mutant banks, we chose to use biosensors for their cost-effectiveness, and efficiency in assessing a chemical’s concentration, therefore relative enzyme activity.
But first, what are biosensors?
Biosensors can be thought of as any biological device capable of sensing: molecules, ions or physical parameters such as pH, temperature, magnetic field or pressure. Even though sniffer dogs from a k9 unit or carrier pigeons with their magnetite beak could be considered as biosensors, this term generally refers to particular genetic circuits in the field of synthetic biology.
Biosensors are used for a wide range of applications, from medical diagnosis  to metabolic engineering and have recently begun being used in cell free systems. But they usually operate in a similar manner: enabling expression of colorimetric, fluorescent or selection molecules in response to the presence of the desired compound. This is the behavior that allows their use in quantification or screening methods .
We can find many types of biosensor architectures, but the ones most used in biotechnology are the transcription factor based biosensors (TFB). TFB natively exist in all organisms, bacterial two-component signaling systems being a perfect example. These types of biosensors are particularly useful for screening processes since they provide appropriate dynamic range and sensitivity . Transcription factor based biosensors are usually built with well characterized transcription factors (like OxyR for hydrogen peroxide) but you can dig for ‘your favorite’ transcription factor in databases such as DBD or RegPrecise where there is a huge amount of predicted transcription factors that can be used to develop biosensors for ‘your favorite’ compound.
Design & Construction
Transcription Factors and Promoters
In order to build a functional transcription factor based biosensor, able to assess ‘our favorite’ compound D-Psicose, we needed a transcription factor and an inducible promoter. Searching databases like RegPrecise, we found the PsiR of Rhizobiales. PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose. This implies that PsiR is potentially capable of binding a consensus sequence in the promoter region and preventing transcription of the regulated promoters in the absence of D-Psicose, in a similar manner to the way LacI does in the absence of allolactose (or the synthetic IPTG).
PsiR occurs naturally in four different Rhizobiales species (Agrobacterium tumefaciens, Rhizobium leguminosarum, Sinorhizobium fredii, Sinorhizobium meliloti) where it regulates an operon while also self-regulating its expression. In all these species, the genetic context is very similar: the PsiR gene precedes the operon (that starts with the PsiA gene) and is in the opposite direction (as an example, see this region in the Agrobacterium tumefaciens genome). Thus, the promoter regions of PsiA and PsiR genes are overlapping and face the opposite direction.
Using the BPROM webserver, we identified the -10 and -35 boxes that are in very close proximity to the predicted PsiR binding sites (the full annotations can be found in Parts Registry pages of these promoters (BBa_K2448010, BBa_K2448011, BBa_K2448012, BBa_K2448013, BBa_K2448014, BBa_K2448015). Knowing this, we extracted the 400 bp sequence upstream of PsiA and PsiR genes in each species in order to get 8 different biosensors theoretically psicose sensitive.
Since PsiR is a LacI family transcription factor, we chose to engineer a well-known LacI regulated promoter, pTacI, in order to make a minimal but strong psicose inducible promoter . With the help of databases and literature, we identified a 20 bp consensus sequence on which PsiR binds and replaced the LacO site of pTacI by the consensus sequence, hence creating a new promoter: pPsiTacI (BBa_K2448016).
We also designed pPsiTac2 (BBa_K2448050) which differs from pPsiTacI by having also a second binding site for PsiR upstream the -35 box. This year, Team Newcastle developed an innovative biosensor screening platform, Sensynova. Their system is aimed at characterizing biosensor-based screening systems and generating variants of reporter genes, promoters and sensitivity tuning. To test their platform, they needed to be provided with a functional biosensor based screening system. Therefore, we sent them this promoter along with all the data on our biosensor (See details on our Collaboration page).
Universal Biosensing Chassis
Since these Helix-Turn-Helix transcription factors and promoters were only predicted, we had to build the construct to test their behavior. We talked about our plans with Dr. Fournier, from Sanofi-Aventis, during an interview. He advised us to standardize our building process and screening system with Golden Gate assembly . This particular technique allows fast, efficient and reliable gene assembly, perfect for a high throughput process, to handle multiple cloning of multiple parts in a few steps.
Using Dr Fournier’s suggestions, and in order to speed up the assembly of all our biosensors, we designed the Universal Biosensing Chassis (UBC)  (BBa_K2448023 or BBa_K2448024). This construct aims to provide an answer to the lack of rapid and reliable building methods for transcription-factor based biosensors. Using this chassis, one will only need a suitable transcription factor, able to bind to the molecule of interest, and its related promoter. By its design (Figure 1), the UBC allows fast cloning: depending on what is to be inserted into the UBC and one’s mastery of the Golden Gate Assembly, a functional biosensor can be obtained in less than a week (for more information, see Our Best Composite Part page).
Built on the UBC by replacing mEmerald with codon optimized PsiR and LacZ with pPsi. The biosensors we created in a pSB1C3 plasmid work in the following way: when pTacI is induced by IPTG, it drives the transcription of the PsiR gene coding for the PsiR protein which is predicted to be a transcription factor able to bind D-Psicose. If D-Psicose is present in the cell, the transcription factor will bind preferentially to it and thus it becomes inactivated. The repression of the related promoter pPsi will be released which will enable the transcription of a fluorescent protein, mCherry. If D-Psicose isn’t present in the cell, PsiR will bind to pPsi, preventing any transcription of mCherry.
The characterizations of all the biosensors have been performed following the protocol described in the ‘Protocol’ session of our wiki and presented in the Parts Registry pages of each biosensor (BBa_K2448025, BBa_K2448026, BBa_K2448027, BBa_K2448028, BBa_K2448029, BBa_K2448030, BBa_K2448031 and BBa_K2448032). All tests were performed in technical duplicates and biological triplicates (Figure 2). Fluorescence measurements (mCherry) have been normalized on cell density (OD600nm). Raw data are for all biosensors are available here (Download)
The main goal of the characterization is to determine which biosensor is the most suitable for our screening process. In order to be able to efficiently use our biosensors for real applications, it’s mandatory to evaluate certain parameters:
Optimal Measurement Time
The characterization of the biosensors allowed us to determine many important parameters. For instance, running the experiment for a long period (almost 18 hours) gave us an estimation of the optimal measurement time.
To estimate this duration, we looked at the raw data of all the biosensors and observed that it takes on average around 9 hours to get an observable signal for the lowest concentration of inducer. It means that sensitivity threshold and consequently maximum accuracy is reached 9 hours after induction.
Since we want to detect and measure D-Psicose concentration between 1 mM and 300 mM, we need a biosensor able to get maximum accuracy in this range of concentration in a minimal time. Taking into account the raw data, we can estimate for the majority of our biosensors that if D-Psicose concentration is above 10 mM, a 6 hours incubation after induction would give relevant results.
All the biosensors show a basal expression between 200 and 1800 arbitrary units of fluorescence at 9 hours post-induction. This basal activity even without psicose in the media is due to the imbalance between the amount of PsiR transcription factor available and the pPsi promoter strength. Even when PsiR is produced, the transcription factor can’t totally prevent the transcription from happening. Biosensors with low basal activity are also the ones with the worst sensing abilities. Since we care more about fold change than absolute value of fluorescence, basal activity won’t be a criterion of choice in selecting the best biosensor.
Determining the dynamic range of our biosensors will give us an estimation of their sensitivity, their maximum and their potential use. What we can observe in figure 3 is a rising curve from 1 mM to 300 mM. This gives us two important pieces of information:
- First, the results show that all PsiR seems to interact with psicose behaving as predicted for all species. Similarly for the pPsi promoters, even for pPsiTacI, that seems tightly regulated by PsiR under psicose induction, all behaving as predicted.
- Second, the dynamic range of the biosensors is very similar for every one of them, and appear to go from 1 mM to 300 mM psicose. Given that, we could use any of them in real applications for our bioscreening protocol to assess the production of psicose ranging from 1 mM to maximum 300 mM.
Each biosensor show a particular fold change and linearity profile :
- pPsiA-PsiR from Agrobacterium tumefaciens (BBa_K2448025): Perfect foldchange and perfect linearity in range of concentrations corresponding to bioproduction range (1 mM to 300 mM psicose) (Figure 4)
- pPsiR-PsiR from Agrobacterium tumefaciens (BBa_K2448026): Saturation at high concentration therefore cannot show enzyme improvements, the foldchange is very low (Figure 5).
- pPsiTacI-PsiR from Agrobacterium tumefaciens (BBa_K2448027): Perfect foldchange and satisfactory linearity in range of concentrations which correspond to bioproduction range (1 mM to 300 mM psicose) (Figure 6).
- pPsiA-PsiR from Sinorhizobium fredii (BBa_K2448028): Early saturation upon increasing the concentration. The foldchange is very low. Although it shows more sensitivity (responses in lower concentrations), the extremely weak foldchange of the signal makes this biosensor a bad candidate for screening (Figure 7)
- pPsiR-PsiR from Sinorhizobium fredii (BBa_K2448029): Perfect foldchange in the signal but it tends to saturate at high concentrations. This biosensor is still suitable for screening (Figure 8).
- pPsiA-PsiR from Sinorhizobium meliloti (BBa_K2448030): Early saturation with increasing concentration combined with a very low foldchange (Figure 9).
- pPsiR-PsiR from Sinorhizobium meliloti (BBa_K2448031): Early saturation upon increasing the concentration with very low foldchange. This biosensor shows the best sensitivity but the very weak foldchange of the signal makes it a bad candidate (Figure 10).
Three biosensors would be adequate for screening with regard to their sensitivity, fold change and linearity: First pPsiA-PsiR A.t (BBa_K2448025), then pPsiTacI-PsiR A.t (BBa_K2448027) and finally pPsiR-PsiR S.f (BBa_K2448029). Among those three pPsiA-PsiR A.t (BBa_K2448025) demonstrates the best linearity and fold change simultaneously making it the best candidate for production/enzyme screening.
Influence of Fructose
Testing our psicose biosensor under real application conditions is vital. We aim to measure the concentration of psicose in high fructose level media to observe the conversion of fructose into its epimer, D-Psicose by the D-Psicose 3-epimerase from C. cellulolyticum. But to achieve this, our biosensor pPsiA-PsiR A.t (BBa_K2448025), and especially our transcription factor, has to be highly specific to psicose, in order to produce mCherry in proportion only to the concentration of D-Psicose.
We can see on the graph presented in Figure 11 that fluorescence intensity and fructose concentration are not correlated since increasing concentration of fructose doesn’t influence the fluorescence. According to figure 11, fructose doesn’t influence the biosensor behavior since mCherry production isn’t a function of fructose concentration in the media. This finding implies that our transcription factor doesn’t bind to fructose and that our biosensor can be used in high fructose level media to measure psicose concentration. Therefore, the pPsiA-PsiR A.t biosensor is suitable for assessing the activity of D-Psicose-3-Epimerase converting fructose into psicose.
In order to finely characterize the pPsiA-PsiR A.t biosensor (BBa_K2448025), we assessed the effect of IPTG on the fluorescence for a fixed concentration in psicose (Figure 12). The outcome is that IPTG, regardless of its concentration, doesn’t significantly influence the production of mCherry. It means that the PsiR amount in the cell is constant. Our hypothesis is that this phenomenon is due to the pTacI promoter leakiness, leading to the steady production of PsiR. Since our biosensor is carried by a high copy plasmid (pSB1C3), the number of lacO sites of pTacI is much bigger than the number of LacI molecules available in the cell. Even if this finding isn’t an issue for our experiments, this has to be considered.
This issue can be addressed by performing the characterization of this biosensor in our improved backbone, the pSB1C3_LacIq plasmid (BBa_K2448038), that we designed for exactly this type of situation. pSB1C3_LacIq has a built-in LacI coding sequence under the control of a mutated version of its own natural promoter known as LacIq which leads to a 10-fold increase in LacI expression compared to the natural promoter.
To summarize, at the end of this step, we have 7 different psicose biosensors well characterized. We know that the best of them is pPsiA Agrobacterium tumefaciens – PsiR Agrobacterium tumefaciens (BBa_K2448025) for its dynamic range, perfect fold change and linearity. Fructose is proven to not influence the biosensor behavior. With 9h incubation post induction, this biosensor is ready to be used in a screening process to efficiently detect and quantify psicose in order to look for the best enzyme activity.
-  Courbet A, Endy D, Renard E, Molina F, Bonnet J. Detection of pathological biomarkers in human clinical samples via amplifying genetic switches and logic gates. Sci Transl Med (2015) 7, 289ra83.
-  Rogers JK, Taylor ND, Church GM. Biosensor-based engineering of biosynthetic pathways. Curr Opin Biotechnol (2016) 42, 84-91.
-  Mahr R, Frunzke J. Transcription factor-based biosensors in biotechnology: current state and future prospects. Appl Microbiol Biotechnol (2016) 100, 79-90.
-  de Boer HA, Comstock LJ, Vasser M. The tac promoter: a functional hybrid derived from the trp and lac promoters. Proc Natl Acad Sci U S A (1983) 80, 21-25.
-  Engler C, Marillonnet S. Combinatorial DNA assembly using Golden Gate cloning. Methods Mol Biol (2013) 1073, 141-56.
-  Brophy JA, Voigt CA. Principles of genetic circuit design. Nat Methods (2014) 11, 508-520.


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I know they're a rival, so I think that's why I'm going to be geared up for it. It's going to be a fun time playing against these guys. It's going to be a lot of hard work, but I think it's going to be worth it.
- Paul Gaustad
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22 November 2018
The Taiwanese OEM HTC financial situation isn't good and with each passing quarter, the company's financial results are getting worse. Despite all this loss, the company is still getting ready to announce new phones. However, instead of releasing a new flagship phone, the company is going to announce another entry-level phone.
Back at IFA this year the company launched U12 Life as the new mid-range offering. Now the company is getting ready for another budget-friendly phone. The device bearing model number 2Q72XXX appeared at the Bluetooth SIG. The listing confirms the device is an entry-level phone running on the Qualcomm's Octa-core Snapdragon 435 SoC.
The Snapdragon 435 chipset was announced a couple of years ago alongside the 425 and 625 lineups. The chipset is built on the 28nm process, its an Octa-core SoC with max clocking at 1.4Ghz. The Adreno 505 is on board as GPU. It has the same GPU like the latest Snapdragon 439 chipset. Another important aspect is Snapdragon 435 chipset only supports 16:9 display which means the HTC phone will have thick bezels display. The device is expected to go official by the end of this year. We can expect more details regarding the phone will appear online in the coming days. Stay tuned.
28nm process, the 1.4Ghz as the max clocking speed, and the Snapdragon 435 all point towards an entry-level phone in the offing.
HTC is brave enough to try their luck with an entry-level phone in such a bad financial conditions.


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domain: entertainment


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Very well-kept hotel only 20 minute drive from Monarch Mountain. Hotel staff was very friendly and the continental breakfast had a great spread including omelets, waffles, fruit, and pastries. Will definitely plan to stay here again in the future.
Hampton Inn - Suites Salida is located in the Heart of the Rocky Mountains adjacent to the longest continuos string of 14 000 …More
My family (two adults, two kids) loved staying at this great vintage "mo-tel". During the week's vacation traveling around Colorado, it was our favorite. The owners went out of their way to make us comfortable. The hot tub in the middle of the parking lot is just plain fun -- really!! If you want a change from the ordinary chain hotel, try this place, and support keeping these independent places in business!
This charming/ independently owned motel is centrally situated at Highway 50 and D Street in Salida/ Colorado. Walk or drive …More
*For these trips, trip savings is at least equal to the cost of the flight or hotel if booked separately. Note: For trips including flights, you will be charged by the airline for the flight, but your savings on the trip will equal the cost of the flight.

domain: geography


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The parent company of SsangYong Motor UK, Bassadone Automotive Group, has appointed John Chisholm as director, financial services.
In this newly-created position, Chisholm will be responsible for managing the group’s provision of financial services across Europe, and particularly for SsangYong in the UK.
He was previously CEO of financial services at Mitsubishi UK, where he established retail finance and contract hire operations, before subsequently going on to run them for more than 20 years.
Nick Laird, managing director of SsangYong Motor UK said: “As we develop both our fleet and retail business, it is essential we provide customers and dealers with first-class financial services, especially contract hire. This will play a big part in achieving sales success of the new Rexton and the Musso pick-up, which arrives mid-year.”
Established in 1954, SsangYong is Korea's oldest vehicle manufacturer and only 4x4 and SUV specialist producer. It has 65 dealers covering England, Northern Ireland, Scotland and Wales.


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domain: finance


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March 4, 2018
Dylan's Music: Finding a Voice at NWS
Miami’s own Dylan Zediker may be classical music’s biggest fan.
At three years old, Dylan spoke only 10 words and was diagnosed as being on the Autism spectrum. He began speech and behavior therapy, but his family noticed an immediate positive response to both music and dance. His parents, Garrett Zediker and Maria Molina, began playing YouTube videos of classical music, where Dylan quickly became enamored with Johann Strauss’ The Beautiful Blue Danube waltz and André Rieu.
Dylan has never looked back.
“It’s fascinating to observe the role that music has played in both his life and progress,” said Garrett. “It seems music is an inspiration that makes him work hard in his school work and training.”
From there Dylan fueled his new-found passion with the Berlin Philharmonic’s Digital Concert Hall, where he would stream live concerts at home, and learned about one of the Philharmonic’s horn players, Sarah Willis. Dylan became an immediate fan of Sarah’s Music—her Deutsche Welle TV show—as well as her Horn Hangouts.
After a year and a half of watching everything he could, Dylan was determined to meet his musical hero. Sarah, a frequent Visiting Faculty member at NWS, coordinated a meeting with Dylan at the New World Center.
On February 12, Dylan, now 9, donned his suit with flowers and Valentine’s candy from Dylan’s Candy Bar to meet Sarah and NWS Brass Fellows for an unforgettable afternoon.
This was Dylan’s first time to closely interact with Fellows by sitting on stage while they performed, learning about their instruments and even conducting their ensemble.
Afterward, Sarah took Dylan outside to SoundScape Park with their horns.
“I felt very honored and was touched to see how much meeting me and the NWS Brass Fellows meant to Dylan,” said Sarah. “It was important for me to take time to go out in SoundScape Park and play horn, just the two of us together. He didn’t say much, but his eyes shone and he smiled and smiled. These musical experiences live on inside a child and I think his afternoon with us was something he will never forget. I am so happy and grateful to be able to give back the gift of music in this way, and now Dylan and I are friends for life!”
Dylan—no stranger to NWS—first started attending our Concerts for Kids in 2015, but was first overwhelmed by the stimuli inside the concert hall. “We would sit outside to listen. The NWS ushers were so caring; they allowed Dylan to explore the hall between performances,” said Maria.
“Dylan wants to be a conductor. He calls MTT his hero and NWS Conducting Fellow Dean Whiteside his buddy. Standing on the conductor’s podium was a dream come true for him.”
“Dylan has embraced everything about NWS,” said Garrett. “He gravitates to every event and idea. If he is not actually attending, then he is reading about the events and listening to the sound clips online.” Dylan also loves WALLCAST® concerts, and now enjoys them from the comfort of NWS’s Sensory-Friendly Environment, which Maria calls a “game changer” for Dylan. “He wants to be in there and it’s a wonderfully supportive and understanding environment.”
“Over the past several years we attend all sorts of concerts and WALLCAST® concerts at NWS,” said Garrett. Literally every chance we get. In fact, sometimes we sit out front in SoundScape Park during the day and play music, share a picnic and talk about life. Interestingly, Sarah Willis had no way to know that NWS, including the front lawn, is one of Dylan's "happy places" where he becomes relaxed and focused. Our recent adventure at NWS was an inspiration of the sort that changes lives.”
“I’m so proud of him,” said Maria. “He still struggles, but music erases all of that. It makes him feel great and confident. He’s worked so hard to get here; we’ll do everything we can to foster this passion.”
“I am certain that the New World Symphony was the threshold that opened the door to a vast world of inspiration to him,” said Garrett. “NWS has inspired his mind and been a solid and consistent reason he has progressed so far. His continued focus and inspiration—fueled by NWS and the many musicians that grace our community with their talent—is a blessing to us.”
Dylan’s Favorites Playlist:
Tchaikovsky: Swan Lake
Bernstein: Mambo from West Side Story
Beethoven: Symphony No. 9
Prokofiev: Montagues and Capulets from Romeo and Juliet
Michael Jackson: Smooth Criminal performed by David Garrett
Debussy: Clair de Lune
Dylan and mom Maria enjoy the Sensory-Friendly Environment in October 2017.
Select photos courtesy of Garrett Zediker and Maria Molina.


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Can be found HERE
Not really my thing, but someone here might have been looking forward to it.IMAGE COMICS HC
Written, art and cover by Erik Larsen, Todd McFarlane, Marc Silvestri and Jim Valentino.
At last! After years of waiting, the much-anticipated Image Comics HC is here! The four remaining Image founders return to the characters that made them sensations for a celebration of the creation of Image. The never-before-seen origin of Savage Dragon! A turning point in the life of Spawn -- featuring Todd McFarlane's first full-length comics work in nearly 10 years! The final fate of CyberForce! The future of ShadowHawk! Plus: an introduction by former Image Executive Director and legendary Beanworld creator Larry Marder and a quartet of special features by each of the founders covering Image -- past, present and future.
128 pages, Nov. 2, $24.99 (Limited slipcase, $75).
BATTLE POPE, VOL 1: GENESIS TP
Written by Robert Kirkman, art and cover by Tony Moore and Val Staples.
If you missed out on Battle Pope the first time around, now is your chance to find out what everyone was talking about! It's the end of the world and few are worthy of passage to heaven, not even the Pope. God leaves behind Saint Michael, the leader of his army, to act as a guardian for the humans. He fails, and is held captive by Lucifer. God then enlists the aide of the Pope to rescue Michael, and leaves his son Jesus H. Christ to assist him in his quest. Now presented in full color. Collects Battle Pope #1-4.
120 pages, $12.99, in stores on Nov. 30. [/QUOTE
Fun stuff ^^
It's so good... I need my fix...THE WALKING DEAD, VOL. 4: THE HEART'S DESIRE TP
Written by Robert Kirkman, art by Charlie Adlard and Cliff Rathburn, cover by Tony Moore.
Life in the prison starts to get interesting for Rick Grimes and the rest of our survivors. Relationships heat up, fizzle out, and change entirely almost overnight. By the end of this volume, relationships between key characters are radically changed, setting the stage for future events in The Walking Dead. Collects The Walking Dead #19-24.
136 pages, black and white, $12.99, in stores on Nov. 16.
Judging by THIS cover everything just might be...THE WALKING DEAD #25
Written by Robert Kirkman, art and cover by Charlie Adlard and Cliff Rathburn.
Everything is different now.
32 pages, black and white, $2.99, in stores on Nov. 16.


TWO MCM METAL AND BRASS FLOOR LAMPS
Condition:The electrical components are not guaranteed; will need to be rewired for use. The smaller lamp with minor oxidation to the metal and a small area of adhesive residue, the shade in overall good condition with minor wear. The larger lamp with minor wear to finish otherwise in overall good condition.
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