Well, uh, good news for him?
After the Grand Jury decided against indicting officer Darren Wilson in the death of Mike Brown, the former Ferguson policeman sat down with George George Stephanopoulos and he admitted two very big things.
No, not that he regrets shooting the young man to death, nor that he would do anything differently if he were in that situation again.
[ Related: Officer Darren Wilson Breaks His Silence! ]
He confirmed for the world that he DID get married while he was on paid leave AND that his wife is currently pregnant with their first child. We'll let that sink in.
How stinkin' precious!!
Tila Tequila is so in love with her baby girl Isabella Monroe Nguyen and it shows!
The former reality TV star gave birth to the bouncing bundle last week, and kept Isabella under wraps until now!
[ Related: Tila Tequila FINALLY Gives Birth To Her Baby! ]
On Tuesday, the 33-year-old model took to her website to share three photos of her baby, including a super intimate one of her daughter breastfeeding!
She provided an update on her few days of motherhood, writing:
We know someone who would be freaking out over this latest friendship revelation!
And her name is Kristen Bell!
[ Related: Kristen Bell Has An Emotional Breakdown Before Meeting A Sloth On Her B-Day! ]
A human five-month-old baby named Alia is best friends with a sloth who met her when she was only 2 days old!
And ever since that day -- the two have been inseparable!
Read More on
We hope she has a wonderful Thanksgiving with her loved ones, but it sounds like it won't include her family from Here Comes Honey Boo Boo.
Anna "Chickadee" Cardwell has revealed in an interview that she will NOT be spending the stuff-your-face holiday with Mama June and the reasons are obvious…
But if you haven't been reading the news at all in the past month, it's probably due to all the drama Anna has had to endure because of the scandal around Mama allegedly hooking back up with Mark McDaniel — and ex that molested Anna as a child.
Instead Anna will be with her husband Michael and her daughter Kaitlyn, and that's just fine with her!
Here's what she said:
What did the five fingers say to the face?
Well, Chris Soules found out the answer to that riddle on the newest promo for the new season of The Bachelor!!! Chris Harrison had the answer! LOLz!
This past Monday ABC released the newest promo for the reality show that people just can't get enough of, and it was filled with lots of drama — like a PREGNANCY SCARE!!


The house is located on a terrace and was originally of two-storey, load-bearing masonry construction. A two-storey extension was built later, and another, single-storey extension was subsequently added.
The rear extensions was modified so that the kitchen, services and toilets could be rearranged. The existing downstand beam was removed at 1st floor. The ground outside was built up in order to create an attractive terraced area.
Rise was appointed to carry out the structural design of the retaining wall, the ground bearing slab, new openings and roof. Due to the large openings at the existing ground-floor level, a portal frame was installed to guarantee the stability of the building.


text:
The Audiovisual Library holdings comprise a hybrid collection with both analog and digital records. Included are films, video recordings, audio recordings in a wide variety of systems and formats. In addition, the Photo Library also holds approximately 800,000 photographs. The unique analog and historic collections are being digitized selectively as resources are made available also.
A United Nations database.

domain: library


we already saw this message once.
No answer does not mean automatically that nobody is receiving your mail.
Is this by chance based on a RedHat version ?
Or on people having the same brain-damaged but support-reducing idea to add
following entry to /etc/hosts during installation:
> -----Original Message-----
> From: rdesktop-users-admin@...
> [SMTP:rdesktop-users-admin@...] On Behalf Of Jack
> Sent: Friday, October 08, 2004 10:26 AM
> To: rdesktop-users@...
> Subject: [rdesktop-users] Thinstation / Rdesktop but no IP-address
> in TS Manager
> Hi there,
> Can someone help me with the following problem?
> I'm using a prebuilt CD from the download
> t-ISO.zip>. I use a floppy for the thinstation.conf.user. It all works
> marvelous except only my printing cost registration software does not
> detect printjobs when I print in a Rdesktop session. I think (thanks to
> Matthew E. Bernold) that the problem occures because the session has the
> "wrong" IP-adres...
> In attached screenshot you can see that in Terminal Services Manager of my
> Windows2000-server the IP-adres of my Rdesktop station "LatiThin" is
> 127.0.0.1 in stead of its real ip adres which I cannot detect but has to
> be in the range 192.168.27.x because that's what my dhcp server gives.
> Does anyone has an explanation?
> << File: thinIP2.gif >>
Get latest updates about Open Source Projects, Conferences and News.


text:
Vendors attribute higher cost of foods to levies, fares
Due to very poor states of rural roads, transporters charge unreasonable fares to convey farm products to cities.
Therefore, regardless of the production season, prices of fresh farm products in metropolitan marketplaces are high.
Seasonal harvests usually lower household spending on food items because of the law of demand and supply. Farmers do supply all fresh farm products to the marketplaces because they lack storage facilities or value chain development.
This often prompts excessive supply over demand at certain periods of the year. For instance, tubers of yam are usually cheaper between August and October.
However, a survey in the Lagos metropolitan marketplaces revealed that prices of these farm products are relatively too expensive.
Speaking with The Guardian, Mr Ali Suleman, a supplier of yam and potatoes in Ajamgbadi marketplace, Ojo, Lagos State, said farmers had been making things difficult for the bulk buyers, especially in the area of prices.
He said: “The formation of farmers’ associations leads to price hikes of farm products. Some farmers cannot sell their farm products without recourse to the price tag of a farmer’s association. That makes the bargaining process very difficult.”
Again, transportation fare and government levies using Agberos to collect levies from transporters is a big challenge that causes an increase in the prices of fresh foods. The transporters transfer the cost to a food dealer or aggregator, and in turn, the dealers transfer the cost to consumers.
As a result of the foregoing, Ali emphasised that he could no longer buy a bag of potatoes at the rate of N4,500 or N5,000 as the price has increased to N10,000.
Corroborating the fact, Salihu Kehinde, a yam seller at Mile 12 marketplace, said sometimes, festive periods do influence an upward movement of prices of fresh foods. For example, during Sallah, a set of tubers of yam goes for N13,000, which costs N9000 without festive periods.
The country is by far the largest producer of yam, accounting for 70-76 per cent of the world production. Almost 60 per cent of states in Nigeria cultivate and harvest yam, but the challenges farmers face during the period of planting and harvest contribute to the expensive nature of the commodity in some states.
Mrs Chioma Onyeka pointed out that fresh vegetables are expensive because of the limited vegetable farmers in Lagos State, and some of the vegetables are transported from another state, making it expensive for the seller and consumers.
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domain: agronomy


text:
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domain: medical


text:
Fascinating people making headlines.
July 11, 2012 by Eudie Pak
Do you’ve got the moves like Jagger? Do you’ve got the moves like Jagger? Do you’ve got the moo-oo-oo-oo-ves like Jagger?
July 12th marks the 50th anniversary of The Rolling Stones’ first gig, and with that milestone, the band is releasing their personally...also in this article
July 30, 2012 by Leila Taha
Jesse Owens, a record-breaking Olympic sprinter and the best athlete of his time, spent much of his life struggling with issues of race. Unlike other athletes of...also in this article
July 9, 2012 by Eudie Pak
Actor Ernest Borgnine, known for his Academy Award-winning performance in Marty, as well as his TV role in the 1960s sitcom McHale's Navy, died Sunday from kidney...also in this article
Film Actor, Theater Actor, Television Actor / 1917 - 2012
Actor Ernest Borgnine’s role in the film Marty transformed him from a stereotyped character actor to a leading man, earning him an Academy Award as Best Actor.
Guest ContributorJuly 5, 2012 by Scott Cooperstein
The American South is a hotbed for literature, and many of America’s most famous novels have been written by Southerners. From Mark Twain and the invention of the...also in this article
July 27, 2012 by Eudie Pak
Biography.com's "Week in Review" highlights interesting people who've made the news this past week—thanks to their endeavors, big ideas, triumphs, importance in...also in this article
July 2, 2012 by Julien McNab
The producers at BIO.com review hundreds of photos of famous figures each week, from award-winning actors and popular singers, to headliners and scandal makers. While we're digging through these archives, we often come across amazing photos that we're...

domain: entertainment


text:
“Love is patient and kind; love does not envy or boast; it is not arrogantor rude. It does not insist on its own way; it is not irritable or resentful;it does not rejoice at wrongdoing, but rejoices with the truth.Love bears all things, believes all things, hopes all things,endures all things.” I Corinthians 13:4-7
I made a cake for my Valentine this year. Thought I’d try one of his favorite cakes: The Red Velvet Delight. 🙂
A ring holder makes a for special gift idea.
No time for baking and need a cake for a special occasion? My dear friend Daris, introduced me to the best Red Velvet Cake that money can buy from Nothing Bunt Cake . You will only want more!
I just want to say thank you to all you ladies who contributed to the beautiful baby shower last friday. A special thank you goes out to my gracious host, Silje. I was truly blessed by your love and efforts! I’m thrilled that baby showers are becoming a growing trend in Norway. I couldn’t resist getting involved in making something myself, so I sent my darling sister on a shopping spree to get everything necessary to make cupcakes for a little princess. Our efforts were inspired by Norway’s most famous blogger, Emanuela (with Passion 4 baking). If you need more ideas, or simply want the recipe, click the link. The kitchen was filled with delicious treats including Bella’s irresistible chocolate cake. A perfect scene for hungry eyes. An accessory that I couldn’t help but notice in her kitchen was the vintage Roberts radio RD60. This amazing device was a great addition to her quaint and lovely place. To all you music lovers who appreciate retro design, this radio will not disappoint. She is top notch, but pricey.
Hopefully i can convince hubby that this one is just what “he” needs.
As usual, I was shopping for groceries after work and took my obligatory “exited-expecting-mom-stroll” down the baby isle. While I was looking at binkies for the millionth time, I reached the same disappointing conclusion as always: that I needed to go to a expensive boutique to get the ones with a little personality (that does not include Disney or Dora the Explora)!
I found myself staring at a couple of neutral pacifiers which promised that 1.) they had the perfect nub for a little one to suck on (only in natural and good materials of course) and, 2.) with a breathable plastic rim, which is much better for sensitive skin. Very impressive I must say, but the only drawback was that those binkies’ designs were making me yawn on the inside. sigh.
Then the thought hit me. It would only take a couple crystals, some half-pearls, strong glue, and some stunning ribbons to outshine the contenders. Easy.
1. It’s helpful to create your design before you start gluing on your gems. Use a strong glue that will make it impossible for a child to loosen the crystals.
2. Apply your crystals with a pair of tweezers.
To view more images of binky bling visit the Gallery
This year I wanted to step it up a notch and make my Easter eggs nicer than your average Joe’s. The idea was born as I imagined my eggs looking like oversized dinner party earrings. In my mind I went on a journey making a mix of hand-painted, dotted pink, and gold-leafed distressed looking eggs; both batches bejeweled with antique gems, water pearls, and faded pink stones (all real off course!).
No really, it was an inexpensive and fun way to decorate, and I hope it generates more beautiful ideas for my favorite holiday — Easter!
1. Go outside and find your green! Here in Oslo, the trees are budding and the colors are radiant.
2. Poke your eggs with a needle at both ends, blow the content of the egg out, and then whip up a big omelet for your family!
3. Paint your eggs with a base color before you add your design (unless you are using watercolors). While you let the base dry, you can play with ideas on a separate piece of paper.
4. For my pink eggs I used quality acrylic paint and a color-wheel to help me coordinate my pallette. I dotted the eggs with a toothpick.
5. Tying it all together was simple; I threaded everything on some beautiful yellow straw, resembling cute little fox tails. Lastly, I hung my oversized earrings on branches like it was Christmas!

domain: hobby


text:
On an ordinary Tuesday afternoon, the very extraordinary Tessa's Bakery opened the doors of its brand new store. The ‘Made like Mom's' bakery chose a unique location in Milnerton, Cape Town, opting to stay near to the loyal client base they've built up, while catering to the growing demand for their delicacies. Having officially launched in 2012 supplying coffee shops and later offering an online store, Tessa's Bakery has been 25-years in the making with humble beginnings in Tessa's very own home kitchen.
Angie White 1 day ago
JML Consulting, which created Pierre Jourdan Wine Popsicles (PJ Pops) in 2016, will launch two gin & tonic popsicles - a G&T Classic and G&T Berry - in September this year.
The 2017 Coca-Cola Food Festival saw over 40,000 public votes cast to select the regional semi-finalists representing South Africa's favourite eateries. The public has a chance to sample these delights in person at the Joburg and Durban regional semi-final events.
Nigerian artist, Peju Alatise, has been announced as the 2017 recipient of the highly coveted FNB Art Prize at the media launch of the 10th instalment of the FNB JoburgArtFair.
Through an innovative partnership showcasing South Africa's diverse leisure tourism attractions, the South African launch of Nollywood action comedy 10 Days in Sun City recently took place at Sun City where the movie was filmed. Directed by South African-based Nigerian director Adze Ugah, the movie is the highest-earning film in Nigeria this year and topped the country's box office for two weeks after its release in June. The movie will be released in 20 cinemas across SA on 25 August 2017.
Winners of the SunshineCo. Women's Month photographic competition were announced on Thursday, 3 August 2017 at the SunshineCo. Studios in Rosebank.
With an illustrious career spanning 40 years, designer, icon, businesswoman, mentor and all-around creative Marianne Fassler remains a formidable force in South Africa's fashion industry.
Lauren Hartzenberg 3 days ago
The BMI Song Camp will run from the 4 September till the 8 September in lead up to this year's Music Exchange (MEX17) at the Academy of Sound Engineering studio complex, in District 6, Cape Town.
Lorna Scott, a driving force behind South Africa's craft gin revolution, had never worked in a distillery before or had any experience in the liquor industry, when she launched Inverroche Gin in 2012 in Still Bay with her son Rohan and daughter Lauren.
The Oyster, Wine & Food Festival takes place on Friday, 1 September to Sunday, 3 September 207 in the Outdoor Arena, Montecasino.
The nominee's list for the 2017 AFRIMA Awards were announced by officials of the African Union Commission, and 13-man jury of the All Africa Music Awards (AFRIMA) on Tuesday, 8 August at the Renaissance Hotel, Ikeja in Lagos, Nigeria.

domain: news


text: Disability Income This calculator estimates your chances of becoming disabled and your potential need for disability insurance. You are viewing this website at a small screen resolution which doesn't support calculators. Click here to view this calculator. Have A Question About This Topic? Name Email Address Question Thank you! Oops! Related Content Inflation & Retirement Estimate how much income may be needed at retirement to maintain your standard of living. Protecting Your Business from the Loss of a Key Person Business owners may be able to protect themselves from the financial consequences of losing a key employee. Put It in a Letter A letter of instruction provides additional and more personal information regarding your estate.

domain: economics


Bi-Fold, High-Cycle Railgates
Bi-Fold, High-cycle models feature folding platforms ideal for dock loading and utilize a steel roller and leaf-chain pulley drive system which maximizes smoothness of motion, endurance, and strength. This medium-duty railgate is ideal for high-cycle applications in any environment. These lifts are built with the highest quality components and place a priority on function and safety.
Steel rollers guide the platform vertically within the rail track and are designed to withstand intensive cycling. Dual sealed, maintenance-free ball bearings ensure the rollers are long-lasting and perform consistently in all climate conditions.


text:
The Cell Theatre, Albuquerque’s boutique home for quality live performance, is proud to present the Albuquerque debut of “Sage and Fool, Part II”, written and performed by Taos artist David Garver. The production is a follow-up to his last sold-out performance “The Age Between Sage and Fool”.
The show includes live musical performances, film vignettes and video transitions.
“A unique multi-media live theatre event. Funny and profound. Don’t miss it!” – Brad Hockmeyer, KTAOS Solar Radio
“Garver emerges as the quintessential wise man, and quintessentially hilarious as well. A one-man masterpiece.” - Mirabai Starr, author and translator, Dark Night of the Soul
Venue: The Cell Theatre, 700 1st St. NW, Downtown ABQ
All seats are $15.
Thanks to the City of Albuquerque, Pay What You Wish ticketing is also available for these performances. To PWYW, link HERE
There is free parking in the private lot on the north side of the theatre.
Box office: 505.766.9412


BERLIN: German Chancellor Angela Merkel said she doesn’t accept Saudi Arabia’s explanation on the death of government critic Jamal Khashoggi, as European leaders pressed the kingdom to come clean on how he was killed.
While President Donald Trump called the Saudi response and admission that Khashoggi was killed at its consulate in Istanbul a “good first step,” Merkel portrayed the “horrific events” surrounding the journalist’s killing as a warning that democratic freedoms are under assault across the globe.
“They still haven’t been cleared up and of course we demand that they be cleared up,” Merkel told a regional convention of her Christian Democratic Union party in eastern Germany on Saturday.
With Republican and Democratic lawmakers in the US rejecting Saudi Arabia’s account on Friday that Khashoggi died during “an argument” in the consulate, Danish Prime Minister Lars Lokke Rasmussen also said he was unconvinced.
“The fact that the Saudis last night confirmed that he died, after previously insisting he left the consulate alive, shows that we haven’t been told the full truth, and we must insist on getting that,” Rasmussen said in Copenhagen after talks with Dutch Prime Minister Mark Rutte.
Rutte said Khashoggi’s killing was “shocking.” He and Rasmussen said they would back an investigation into his death by the United Nations and other Western powers.
Saudi Arabia’s explanation distanced the royal family from a case that has sparked global outrage and roiled the kingdom’s ties with the US and Western allies.
In Washington, Senate Foreign Relations Chairman Bob Corker of Tennessee noted that the Senate has demanded an investigation by the US government. He said in a statement that the Saudis “can undergo their own investigation, but the US administration must make its own independent, credible determination of responsibility for Khashoggi’s murder.”
Merkel, who grew up under communism in what was then East Germany, mentioned Khashoggi’s killing after evoking the legacy of the East German pro-democracy uprising that toppled the Berlin Wall in 1989.
“Yet globally, these freedoms are anything but self-evident,” she said in a speech.
Threat of Violence
With the European Union under attack from populists, Merkel told her party’s delegates in the state of Thuringia that the CDU’s task is to stand up to nationalists forces that want to break up or weaken the European Union, including in next year’s European Parliament elections.
“We see how others are trying to breach taboos, say things openly, throw everything into question,” she said. “We must never allow that. Thoughts turn to words, words turn into action and that leads to violence.”


text:
From Shelton to Rome
Frank Valabek, the father of Redemptus, was born in Vinehrad, Slovakia, one of the seven children of a sharecropper family whose land owners were absent French nobles. Because his family was large and work scarce, Frank walked one hundred miles to Klobuk in the present Czech Republic and found work there in a slipper factory. At Klobuk, Frank lived as a staunch Catholic singing in the choir and leading processions to a local Marian shrine.
On of Frank’s brothers had come to the United States and was working at Endicott Johnson in Endicott, NY. As was the custom in those days, he sent money back home for another in the family to emigrate. Frank was the one chosen but when he came to Endicott, he found there was no work for him. He knew from a neighbor back home of the Fruit of the Loom factory in Shelton, CT, where he found employment. Frank then worked at another plant but was laid off. It was the time of the Depression and Frank secured a position with the Works Progress Administration (WPA).
Frank continued at Shelton his firm practice of his faith. This was manifested in his return to the Czech Republic for the consecration of the cathedral in Prague. When he returned to his home town on this trip, some women arranged for his marriage to Anna Valcik who was from Potec in the present Czech Republic. After the wedding, Frank returned to the United States to earn enough money to bring is wife over. When she came they lived in New York City. From work in the city, Frank obtained a job with a Jewish family upstate New York but was not satisfied there and returned to the city. They later came to Shelton where both Anna and Frank worked.
Redemptus was born at home in Shelton on July 3, 1934 and was baptized Francis. He attended Saint Joseph’s Grammar School and served as an altar boy in the parish. As a youth, he played Mass using his father’s wine in the cup. Throughout his life, he kept in touch with his grammar school classmates.
Redemptus accepted pain in his life and offered it for the suffering of others. This was especially evident when at the age of eleven he impaled his foot on a broken branch. A doctor stitched his leg at home without anesthesia.


text:
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domain: health


text:
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domain: geography


text:
Scent test using Caenorhabditis elegans to screen for early-stage pancreatic cancer
Metrics: PDF 1875 views | Full Text 11232 views | ?
Ayumu Asai1,2, Masamitsu Konno1,4, Miyuki Ozaki1, Koichi Kawamoto1,5, Ryota Chijimatsu1, Nobuaki Kondo1,3, Takaaki Hirotsu1,3 and Hideshi Ishii1
1 Center of Medical Innovation and Translational Research (CoMIT), Graduate School of Medicine, Osaka University, Suita, Osaka, Japan
2 Artificial Intelligence Research Center, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, Japan
3 Hirotsu Bio Science Inc., Chiyoda-Ku, Tokyo 102-0094, Japan
4 Present address: Research Institute for Biomedical Sciences, Tokyo University of Science, Chiba, Japan
5 Present address: Kinnki Regional Bureau of Health and Welfare, Osaka, Japan
|Hideshi Ishii,||email:||[email protected]|
Received: May 07, 2021 Accepted: July 13, 2021 Published: August 17, 2021
Although early detection and diagnosis are indispensable for improving the prognosis of patients with pancreatic cancer, both have yet to be achieved. Except for pancreatic cancer, other cancers have already been screened through scent tests using animals or microorganisms, including Caenorhabditis elegans. While such a method may greatly improve the prognosis of pancreatic cancer, no studies have investigated the same, mainly given the difficulty of collecting suitable samples from patients with early-stage pancreatic cancer. In this study, we organized a nationwide study group comprising high-volume centers throughout Japan to collect patients with very-early-stage pancreatic cancer (stage 0 or IA). We initially performed an open-label study involving 83 cases (stage 0–IV), with subsequent results showing significant differences after surgical removal in stage 0–IA (×10 dilution: p < 0.001; ×100 dilution: p < 0.001). Thereafter, a blinded study on 28 cases (11 patients with stage 0 or IA disease and 17 healthy volunteers) was conducted by comparing very-early-stage pancreatic cancer patients with healthy volunteers to determine whether C. elegans could detect the scent of cancer for the diagnosis of early-stage pancreatic cancer. Preoperative urine samples had a significantly higher chemotaxis index compared to postoperative samples in patients with pancreatic cancer [×10 dilution: p < 0.001, area under the receiver operating characteristic curve (AUC) = 0.845; ×100 dilution: p < 0.001, AUC = 0.820] and healthy volunteers (×10 dilution: p = 0.034; ×100 dilution: p = 0.088). Moreover, using the changes in preoperative and postoperative chemotaxis index, this method had a higher sensitivity for detecting early pancreatic cancer compared to existing diagnostic markers. The clinical application C. elegans for the early diagnosis of cancer can certainly be expected in the near future.
Pancreatic ductal adenocarcinoma (PDAC) is among the deadliest diseases, with a five-year survival rate of 9% . Although surgical resection is the only curative treatment, less than 10% of cases are surgically resectable, and the median survival is only 17–23 months even after successful resection . On the other hand, studies have reported a 5-year survival rate of 80.4% for PDAC smaller than 10 mm (TS1a) and 85.8% for Union Internationale Contre le Cancer (UICC) stage 0, indicating that early detection of PDAC is indispensable for overcoming this refractory disease [3, 4]. Yachida et al. reported that the duration from the onset of pancreatic mutation to the development of metastatic PDAC takes almost 21 years, which consequently provides enough time for detection before progression into the advanced stage . However, imaging modalities have remained the currently available practical methods for detecting PDAC, making it almost impossible to distinguish patients with very-early-stage (stage 0 or IA) PDAC.
Various studies involving genomics, transcriptomics, proteomics, and metabolomics have identified putative molecular biomarkers for the diagnosis of early-stage PDAC, some of which analyzed body fluids, such as feces, urine, and saliva, of patients with PDAC [6, 7]. However, most of these molecular biomarkers still remain unavailable or under development. On the other hand, several reports have emerged regarding canine scent detection of cancers. Since Williams’ first report regarding the ability of sniffer dogs to detect skin cancer, a number of studies have indicated the ability of dogs to detect cancerous lesions, including those in the bladder, lungs, breast, and ovaries [8–12]. However, Williams also reported that canine scent diagnosis may be difficult to introduce into clinical practice owing to the expenses and time required for canine training and education.
Similar to sniffer dogs, the use of Caenorhabditis elegans has been introduced as a new strategy for detecting cancer-associated scents during cancer screening. Aside from having an excellent sense of smell, C. elegans is easy to handle, inexpensive, and quick to breed. The method for olfaction analysis, named N-NOSE (Nematode-NOSE), has been well established as a simple system for observing chemotaxis. These features make this organism ideal for screening tests [13, 14]. Hirotsu et al. reported that wild-type C. elegans displayed attractive chemotaxis toward human cancer cell secretions, cancer tissues, and urine from patients with colorectal, gastric, and breast cancers . This biological diagnosis had a reported sensitivity of 95.8%, which was also acceptable even in patients in early-stage of cancer. Furthermore, reports have shown that this test demonstrated high sensitivity in cases of gastrointestinal cancers and negative changes in the postoperative period [16, 17]. Moreover, this test could discriminate urine in a mouse model of pancreatic cancer . Therefore, this method may be useful for detecting patients with early PDAC. However, how study has yet utilized this method to detect very-early-stage (stage 0 or IA) PDAC mainly due to the extreme difficulty of collecting urine samples from such patients . In the present study, we organized a nationwide clinical group that comprised high-volume centers throughout Japan and prospectively collected serum and urine samples from patients with very-early-stage PDAC (stage 0 or IA) to investigate the clinical value of a cancer detection system involving C. elegans.
Prior to the study on early-stage pancreatic cancer, 83 patients with various stages of pancreatic cancer were investigated in an open-label pilot study (Figure 1). The age, sex, pathological progression, CA19-9 value, and CEA value for the 83 patients are summarized in Table 1. Results of the scent test using urine samples from the 83 patients with PDAC showed a significant decrease in the chemotaxis index of postoperative urine samples at both 10- and 100-fold dilutions (Figure 2A). To evaluate the effects of cancer progression on the scent test, early-stage (0, IA) and late-stage samples (IB–IV) were investigated. Accordingly, both early- and late-stage postoperative urine samples showed a decrease in the chemotaxis index (Figure 2B, 2C). Furthermore, to evaluate the diagnostic performance of the scent test for the presence of tumor tissue (pre-/postoperative), a receiver operating characteristic (ROC) curve was created. Subsequent results showed that the scent test had high performance with an area under the (ROC) curve (AUC) of 0.845 and 0.820 at 10- and 100-fold dilutions (Figure 3).
Figure 1: Schema of the scent test using Caenorhabditis elegans for the screening of pancreatic cancer in the open-label study.
Table 1: Characteristics of patients enrolled in the open-label study
|Number of cases||83|
|Age||71.0 ± 8.7 (41–87)|
|Pathological stage (0/IA/IB/IIA/IIB/III/IV)||5/20/4/28/23/0/3|
|CA19-9||31.0 ± 1279.7 (0.6–8997.0)|
|CEA||2.7 ± 3.8 (0.3–29.0)|
Figure 2: Scent test using Caenorhabditis elegans before and after surgical removal of pancreatic ductal adenocarcinoma. The chemotaxis index was measured in 83 cases. The scent tests were performed in cases of all pancreatic cancers (A), stages 0/IA (B), and IB–IV (C).
Figure 3: Diagnostic accuracy of the scent test using Caenorhabditis elegans before and after surgical removal of pancreatic ductal adenocarcinoma.
Given the success of the scent test in determining the presence of pancreatic cancer in patients with PDAC who showed various progressions before and after surgery, a blinded study was conducted to determine the ability of the scent test to distinguish between patients with early-stage PDAC and healthy volunteers (Figure 4). The age, sex, pathological progression, CA19-9 value, and CEA value of 11 stage 0 or IA cases are detailed in Table 2. Given that all cases had early-stage pancreatic cancer, the tumor marker values were normal in most cases. Among the 17 healthy volunteers, 1 had slightly elevated CA19-9, whereas all the other subjects had normal CA19-9 and CEA.
Figure 4: Schema of the scent test using Caenorhabditis elegans for the screening of pancreatic cancer in the blinded study.
Table 2: Characteristics of patients enrolled in the blinded study
|Early PDAC||Healthy Volunteers|
|Number of cases||11||17|
|Age||70.6 ± 7.8 (58–84)||39.8 ± 8.3 (30–55)|
|Pathological stage (0/IA)||3/8||(–)|
|CA19-9||23.2 ± 18.9 (2–58)||12.7 ± 10.6 (1–41.3)|
|CEA||3.0 ± 1.3 (0.8–4.8)||2.0 ± 1.1 (0.5–4.0)|
The chemotaxis index was measured in 11 early PDAC cases and 17 healthy volunteers. Among the urine samples diluted 10-fold, early PDAC cases had a significantly higher median index compared to healthy volunteers [−0.015 (range, −0.045 to 0.049) vs. −0.038 (range, −0.118 to 0.036); p = 0.034] (Figure 5). When the dilution of the urine sample was further increased to 100-fold, the early PDAC cases tended to have a higher median index compared to healthy volunteers [0.016 (range, −0.078 to 0.149) vs. −0.015 (range, −0.149 to 0.080); p = 0.088].
Figure 5: Scent test using Caenorhabditis elegans for the diagnosis of early pancreatic ductal adenocarcinoma. The chemotaxis index was measured in 11 early pancreatic ductal adenocarcinoma cases and 17 healthy volunteers.
On the other hand, the sensitivity of CEA and CA19-9 were 0% (0/11) and 27% (3/11) respectively, indicating the general difficulty of detecting early-stage pancreatic cancer (Table 3). In urine samples from early-stage PDAC cases in the blinded study, the chemotaxis index decreased after surgery, similar to that in the open-label study, although not significantly (Supplementary Figure 1). However, Table 3 shows that the chemotaxis index of approximately 50% of the patients decreased following surgery (10-fold dilution: 5/11; 100-fold dilution: 6/11), suggesting that the chemotaxis index could be a marker for the sensitive detection of early-stage pancreatic cancer.
Table 3: Patients with early-stage pancreatic cancer
|Age||Sex||Tumor diameter (mm)||Tumor location||Pathology||ly||v||p Stage||CEA (ng/ml)||CA19-9 (U/ml)||Preoperative||Postoperative||Postoperative index − Preoperative index Δ index (×10)||Preoperative||Postoperative||Postoperative index − Preoperative index Δ index (×100)||Days after operation for postoperative sampling|
|N-NOSE index (×10)||N-NOSE index (×10)||N-NOSE index (×100)||N-NOSE index (×100)|
|74||F||4||Ph||Carcinoma in situ||0||0||0||4.8||16.3||0.044||−0.036||−0.080*||0.016||−0.015||−0.031*||33|
|67||F||8||Pb||Carcinoma in situ||0||0||0||0.8||2.5||−0.028||−0.051||−0.023*||0.004||0.003||−0.001*||85|
|84||F||13||Pb||Carcinoma in situ||0||0||0||1.4||7.8||0.020||−0.055||−0.075*||0.048||0.002||−0.046*||38|
Exhaled volatile organic compounds (VOCs) have been reported as biomarkers of certain gastrointestinal disorders, such as inflammatory bowel disease, ulcerative colitis, and necrotizing enterocolitis [19–22]. Studies on VOCs from patients with cancer have identified several substances in the exhaled breath of patients with lung and breast cancer using gas chromatography/mass spectroscopy [23–30]. Although these data are promising, such biomarkers are not commonly used in clinical practice, mainly because of the technical difficulties in measuring exhaled substances. Sniffer dogs have been employed to measure such exhaled substances from patients with cancer [8, 9, 31]. With the observation that sniffer dogs could distinguish patients with cancer through the scent of not only their stools but also exhaled breath, they speculated that cancer-specific substances may be circulating throughout the body of patients with cancer. The present study showed that the cancer detection system involving C. elegans can be an alternative method for measuring cancer-specific substances. This biological diagnostic system seemed to be economical, painless, rapid, and convenient. Therefore, the clinical application of C. elegans may be easier compart to that with sniffer dogs. Considering previous studies on other cancers, several substances may be involved in the scent test using C. elegans, including volatile n-alkanes, such as pentane, hexane, and longer-chain alkanes, derived from carcinoma-associated fibroblasts in breast cancer cases . Moreover, given that the Ras-MAPK pathway regulates meiosis and spout formation in C. elegans, such a pathway has been shown to reduce chemotaxis to odorants, and the exposure of the odorant isoamyl alcohol to C. elegans resulted in the activation of the MAP kinase in olfactory neurons within 10 s, suggesting that the Ras-MAPK pathway may be a candidate pathway involved in scent detection using C. elegans, although the underlying mechanisms for chemotaxis remain to be elucidated [13, 14, 32, 33]. Eventually, recent study proposed that 2-octonone and pentanal levels were increased in urine of prostate cancer patients, compared to healthy controls, suggesting that both 2-octonone and pentanal higher levels in urine may be biomarkers of prostate cancer . Moreover, very recent study of pancreatic ductal adenocarcinoma indicated that possible VOCs and metabolite biomarkers in urine were acetone, 2-pantanone, 4-methyl-2-heptanone, D-limonene, and levomenthol, though both chronic pancreatitis and PDAC shared common VOCs, so in the previous study, the authors could not fully discriminate both chronic pancreatitis and PDAC using GC-TOF-MS . It is suggested that C. elegans in N-NOSE could sense a more complex pattern of VOCs and metabolites in urines and showed a chemotaxis response; that C. elegans may be superior to the GC-TOF-MS with headspace analysis approach with urine samples. Furthermore, a study reported patterns of VOCs in urine of PDAC patients, but without identifying specific molecules . The study showed different patterns of VOCs, which could be distinguished between pre-cancer with acute pancreatitis and advanced PDAC cases, suggesting the measurement of VOCs before and during pancreatic cancer stages may make clear different patterns to healthy controls .
Importantly, this study showed significant differences in the chemotaxis assays of preoperative samples between patients with early-stage PDAC and healthy volunteers but to difference between preoperative and postoperative samples. One possible explanation for this observation could be that patients with PDAC may possess a special constitution that would produce special metabolomes, which may produce a special scent in patients with cancer. This explanation could help us understand that cancer patients possess certain substances, even with a very small cancerous lesion. Another explanation could be the presence of substances that produce scents in patients with cancer and persist even 1 month after surgical resection. Given the lack of data regarding the nature or half-life of the substances, we cannot determine which hypothesis is correct.
Investigating patients with very-early-stage PDAC is important for identifying biomarkers for early-stage PDAC. Paradoxically, however, analyzing samples from such patients is difficult given the rarity of such cases. Melo et al. reported that glypican-1 could detect early pancreatic cancer in only four of the patients with stage I disease . Therefore, we established a nationwide clinical group that involved high-volume centers throughout Japan and prospectively obtained urine samples from patients with very-early-stage PDAC (stage 0 or IA). Particularly, special attention was provided to ensure the quality of the samples in order to obtain uniform and sufficient quality.
This study has several limitations worth noting. First, the number of patients enrolled remains insufficient, despite having established a clinical group to effectively obtain samples. Thus, the evaluation of factors (e.g., age, sex, and other questionnaire items such as inflammatory disorders) between patients and volunteers was limited. In the future, another validation study should be attempted to clarify the utility of this method in clinical practice. Second, it remained unclear whether the scent of the cancer patients was indeed derived from the cancerous lesions or from the metabolic disorders caused by the impaired pancreatic function. Nevertheless, chemotaxis assays have been used in other types of cancers, including colorectal, stomach, and breast cancers, implying that impaired pancreatic function could not have been the reason for the scent of patients with PDAC. Third, the cutoff value of this cancer detection system for early PDAC has yet to be determined. To utilize this system as a screening test for patients with early-stage PDAC, higher specificity is imperative. Despite the high specificity in previous studies [15, 17], an excessive number of participants would need to be sent for detailed examination for PDAC. Moreover, the suitable urine dilution for early PDAC remains under investigation.
In conclusion, the current study observed higher chemotaxis of C. elegans in patients with very-early-stage PDAC, suggesting its potential for use as a standard method for detecting early-stage cancer. Nonetheless, the underlying mechanisms for this chemotaxis should be clarified in order to obtain information that could help elucidate the biological characteristics of cancer.
Materials and Methods
Patients and healthy volunteers
To effectively recruit the exceedingly rare patients with early-stage PDAC (stage 0 or IA), we organized a nationwide group that comprised seven high-volume centers. We recruited stage IA cases based on the 7th edition of the Union for International Cancer Control (UICC), which is more stringent for early-stage cancer. Patients with PDAC were enrolled from October 2015 to June 2019. No restrictions were placed on meals or activities for sampling. The participants were required to be > 20 years old and were asked to complete a questionnaire on factors that could influence the volatile molecules in their urine or serum samples. These factors included age; physical symptoms, such as appetite, weariness, headache, chest, or abdominal distention, cough, bloody feces, constipation, and diarrhea; pregnancy; history of cancer treatment; current use of medicine; alcohol consumption of three or more days per week; and smoking within the previous 2 weeks. The exclusion criteria included participants who had undergone cancer surgery within the previous year, those who were not examined for cancer recurrence despite having undergone cancer surgery more than 5 years previously, and those currently receiving chemotherapy. Given our suspicion that chemotherapy or operation would change urine chemicals in patients with cancer, we sought patients who had not yet undergone any treatment. A serial number was written on each sample tube at the time of collection to identify individual information.
Blood serum and urine sampling
Special attention was provided to maintain the quality of the serum and urine samples, which were harvested early in the morning at each hospital from patients with stage 0 or IA PDAC. The samples were immediately placed on ice and sent by jet air or limited super express train to Osaka University. Each serum sample was separated from whole blood. The separated serum was placed in a 1-mL polypropylene screw cap tube and stored at −80°C until use. Urine samples were processed and stored in the same manner.
Tumor marker determination
Tumor marker concentrations were determined at SRL Inc. Serum CA19-9 and CEA concentrations were determined using chemiluminescent enzyme immunoassay. The cutoff values for serum CA19-9 and CEA were 37 IU/mL and 5.0 ng/mL.
Worm cultures and strains
C. elegans strains were cultured at 20°C under standard conditions on NGM plates with Escherichia coli NA22, which grows in thick layers, serves as a suitable food source for large-scale worm cultures, and have been used for chemotaxis analyses [32, 33, 38, 39]. The strains used in this study were wild-type N2.
C. elegans cancer detection test
A blinded test was conducted with the assayers not knowing the origin of the urine samples. In the chemotaxis assays, 50–100 approximately synchronized young adults participated, after which the chemotaxis index was calculated as previously described [13–15, 33, 38, 40]. We placed 1 μL of urine on two spots on one end of the assay plates (2% agar, 5 mM KPO, 1 mM CaCl, and 1 mM MgSO) and added 0.5 μL of 1-M sodium azide on two spots on both ends of the plates. Animals were collected, washed three times with basal buffer (0.05% gelatin, 5 mM KPO4, 1 mM CaCl2, and 1 mM MgSO4), and spotted to the center of the plates. After 30 min, the number of nematodes was counted, and the chemotaxis index was calculated as follows:
The engaging behavior of C. elegans toward favorite smells would suggest a positive chemotaxis index. Furthermore, the chemotaxis index is correlated with the degree of odor concentrations, and positive peaks appear in accordance with odor concentrations when an attractive odorant is present in the samples. A previous study  analyzed 10-fold diluted urine to more thoroughly investigate the olfactory responses of C. elegans. In the current study, multiple urine concentrations (10-fold and 100-fold) were used to analyzed for the presence of positive peaks in the average chemotaxis indices.
This study was approved by the institutional review board of Osaka University Hospital. All patients and volunteers provided written informed consent.
Differences in characteristics, laboratory data, and tumor markers between control participants and those with cancer were examined using the Wilcoxon signed-rank test (if a pair exists) and Wilcoxon rank-sum test (if the pair does not exist) for dichotomized variables. A p value of < 0.05 was considered statistically significant. Cancer staging was based on the UICC criteria.
We thank Yurika Nakamura for sample management and every member of our laboratory for the fruitful discussions. We thank the following institutes for providing samples from patients under the written institutional agreement of a multicenter study (approval number 664): Koshi Mimori (Department of Surgery, Kyushu University Beppu Hospital, Japan), Takahiro Ochiya (Department of Molecular and Cellular Medicine, Tokyo Medical University, Japan), Hiroaki Nagano (Department of Gastroenterological, Breast, and Endocrine Surgery, Yamaguchi University Graduate School of Medicine, Japan), Itaru Endo (Department of Gastroenterological Surgery, Yokohama City University Graduate School of Medicine, Japan), Tsutomu Fujii (Department of Surgery and Science Faculty of Medicine, Academic Assembly University of Toyama, Japan), Keiji Hanada (Department of Gastroenterology, Onomichi General Hospital, Japan), Satoshi Hirano (Department of Gastroenterological Surgery II, Hokkaido University Faculty of Medicine, Japan), Suguru Yamada (Department of Gastroenterological Surgery (Surgery II), Nagoya University Graduate School of Medicine, Japan), Kiyoshi Hasegawa (Hepato-Biliary and Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, University of Tokyo, Japan), Masaru Miyazaki (Department of General Surgery, Chiba University, Graduate School of Medicine, Japan), Shoji Natsugoe (Department of Digestive Surgery, Breast and Thyroid Surgery, Graduate School of Medicine, Kagoshima University, Japan), Soichiro Morinaga (Department of Hepato-Biliary and Pancreatic Surgery, Kanagawa Cancer Center, Japan), Akio Saiura (Department of Surgery, Cancer Institute of the Japanese Foundation for Cancer Research, Japan; Department of Hepatobiliary-Pancreatic Surgery, Juntendo University School of Medicine, Japan), Hidenori Takahashi (Department of Surgery, Osaka International Cancer Institute, Japan), Michiaki Unno (Department of Surgery, Tohoku University Graduate School of Medicine, Japan), Masakazu Yamamoto (Department of Surgery, Institute of Gastroenterology, Tokyo Women’s Medical University, Japan), Shinji Itoh and Tomoharu Yoshizumi (Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Japan), Yuichiro Doki, Masaki Mori, and Hidetoshi Eguchi (Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Japan; present affiliation of Masaki Mori is Tokai University).
CONFLICTS OF INTEREST
Institutional endowments were partially received from Hirotsu Bio Science, Inc. (Tokyo, Japan); Kinshu-kai Medical Corporation (Osaka, Japan); IDEA Consultants, Inc. (Tokyo, Japan); Kyowakai Medical Corporation (Hyogo, Japan); Unitech Co., Ltd. (Chiba, Japan); Chugai Co., Ltd. (Tokyo, Japan); Yakult Honsha Co., Ltd. (Tokyo, Japan); and Ono Pharmaceutical Co., Ltd. (Osaka, Japan). N.K. and T.H. are employees of Hirotsu Bio Science, Inc.
This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (15H05791; 16K15591; 20H00541).
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38. Yoshida K, Hirotsu T, Tagawa T, Oda S, Wakabayashi T, Iino Y, Ishihara T. Odour concentration-dependent olfactory preference change in C. elegans. Nat Commun. 2012; 3:739. https://doi.org/10.1038/ncomms1750. [PubMed].
39. Taniguchi G, Uozumi T, Kiriyama K, Kamizaki T, Hirotsu T. Screening of odor-receptor pairs in Caenorhabditis elegans reveals different receptors for high and low odor concentrations. Sci Signal. 2014; 7:ra39. https://doi.org/10.1126/scisignal.2005136. [PubMed].
40. Yamada K, Hirotsu T, Matsuki M, Butcher RA, Tomioka M, Ishihara T, Clardy J, Kunitomo H, Iino Y. Olfactory plasticity is regulated by pheromonal signaling in Caenorhabditis elegans. Science. 2010; 329:1647–50. https://doi.org/10.1126/science.1192020. [PubMed].
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- Climate Pledge Arena
SEA -103, O/U 6
Plus-Minus Goals Scored For or Against Total
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Top Adam Larsson News
Kraken's Adam Larsson: Puts up assist
Larsson logged an assist, three hits, two blocked shots and two shots on goal in Saturday's 4-2 loss to the Canucks.
Larsson set up Vince Dunn for the opening tally at 19:56 of the first period. The 28-year-old Larsson is making an impact defensively with 13 hits, 12 blocked and a minus-1 rating through six games, but he's picked up just two assists. Never one for gaudy scoring numbers, the Swede is likely a low-end fantasy option at best.... See More ... See Less
Kraken's Adam Larsson: Sends first helper
Larsson recorded an assist, two shots on goal, two blocked shots and two PIM in Tuesday's 4-2 loss to the Devils.
Larsson set up Riley Sheahan at 10:05 of the second period for the Kraken's first goal Tuesday. The 28-year-old Larsson has filled a defensive role in the top four so far this year. He has an assist, nine shots on goal, 10 hits, 10 blocked shots and eight PIM through five contests.... See More ... See Less
Kraken's Adam Larsson: Agrees to four-year pact
Larsson secured a four-year, $16 million contract from Seattle on Wednesday, Stephen Whyno of the Associated Press reports.
Despite reportedly receiving an identical offer from Edmonton, Larsson will instead join the newly-minted Kraken as the Expansion Draft selection from the Oilers. While not exactly an offensive powerhouse given he's reached the 20-point mark just twice in his 10-year career, Larsson should rack up plenty of hits and blocks and could offer decent value in formats that value defensive stats.... See More ... See Less
Oilers' Adam Larsson: Delivers assist Monday
Larsson posted an assist, two hits, two blocked shots and two PIM in Monday's 4-3 triple overtime loss to the Jets in Game 4.
Larsson logged 39:00 of ice time as the Oilers essentially played a full game's worth of time rotating only their top two defensive pairings. The 28-year-old Larsson had two helpers, nine hits and seven blocked shots in the Oilers' abbreviated four-game playoff run. The 28-year-old is an unrestricted free agent this offseason, but it's believed the blueliner could land a new contract to stay in Edmonton before he would hit the open market.... See More ... See Less
Oilers' Adam Larsson: Registers assist in loss
Larsson posted an assist and three blocked shots in Sunday's 5-4 overtime loss to the Jets in Game 3.
Larsson's shot attempt was deflected by Jujhar Khaira for the Oilers' fourth goal at 4:43 of the third period. The 28-year-old Larsson has contributed an assist, seven hits and five blocked shots in three playoff outings. The Swede was more of a defensive presence in the regular season -- he had 10 points, 166 hits, 128 blocked shots and a plus-2 rating in 56 contests.... See More ... See Less
Oilers' Adam Larsson: Nets lone goal in loss
Larsson scored a goal on two shots in Saturday's 4-1 loss to Vancouver.
Larsson opened the scoring 12:45 into the game, one-timing a Connor McDavid feed at the point for his fourth goal of the year. It ended up being the only offense for the Oilers in their regular-season finale, and the goal snapped Larsson's personal 24-game goal drought dating back to mid-March. Larsson has enjoyed a tremendous season defensively, chipping in with 10 points and 24 PIM in 56 games.... See More ... See Less
Oilers' Adam Larsson: On scoresheet with assist
Larsson provided an assist, two hits and a plus-2 rating in Monday's 6-1 win over the Jets.
Larsson set up Alex Chiasson for the opening tally at 16:22 of the first period. The 28-year-old Larsson had gone nine contests without a point. The Swede is up to nine points, 41 shots on net, 142 hits and 116 blocked shots as a defensive presence in the Oilers' top four.... See More ... See Less
Oilers' Adam Larsson: Generates helper
Larsson registered an assist and a game-high seven hits in Monday's 3-2 overtime win over the Maple Leafs.
Larsson found Kyle Turris to set up the Oilers' equalizer at 1:20 of the third period. The 28-year-old Larsson has eight points in 36 games this season -- that's better than the six points he put up in 49 outings during an injury-plagued 2019-20. The Swede has added 106 hits, 98 blocked shots, 33 shots on net and 16 PIM in 2020-21.... See More ... See Less
Oilers' Adam Larsson: Cashes in on rebound
Larsson scored a goal on two shots and added three hits in Monday's 4-3 loss to the Flames.
A Kailer Yamamoto shot midway through the second period generated a rebound that Larsson buried. He's usually not in a position to capitalize on those plays -- Larsson is a defensive player by nature. The 28-year-old rearguard has chipped in seven points, 29 shots on net, 90 hits and 91 blocked shots in 31 contests. Strangely enough, the Swede hasn't blocked a shot in his last two outings.... See More ... See Less
Oilers' Adam Larsson: Gets on scoresheet with assist
Larsson notched an assist, six blocked shots, three hits and a plus-2 rating in Friday's 6-2 win over the Senators.
Larsson snapped his seven-game point drought with the secondary helper on Gaetan Haas' third-period tally. The 28-year-old Larsson isn't a big part of the Oilers' offense with just six points in 29 outings. He's supplied plenty of non-scoring stats with 91 blocked shots, 86 hits, 26 shots on net and 16 PIM. The Swede will likely always have more value on the ice than in fantasy.... See More ... See Less
Oilers' Adam Larsson: Strong on defense
Larsson notched an assist, eight blocked shots and three hits in Tuesday's 4-3 win over the Canucks.
Larsson's shot attempt in the third period generated a loose puck for Tyler Ennis to send past Canucks goalie Thatcher Demko. The 28-year-old Larsson has gathered five points, 66 blocked shots, 64 hits and 12 PIM through 21 outings. His fantasy value isn't very high in standard formats, but those that count defensive metrics might want to take a look.... See More ... See Less
Oilers' Adam Larsson: Manages helper
Larsson posted an assist and two blocked shots in Saturday's 6-4 loss to the Flames.
Larsson set up William Lagesson for a shot attempt that Jujhar Khaira tipped in for the Oilers' first goal of the contest. The 28-year-old Larsson has been more involved offensively than usual this year, with four points in 13 contests. He had all of six points in 49 outings last year. Larsson has added 42 blocked shots, 30 hits, 10 PIM and a minus-2 rating.... See More ... See Less
Oilers' Adam Larsson: Distributes assist
Larsson notched an assist, three blocked shots, two hits and two PIM in Thursday's 4-3 loss to the Maple Leafs.
Larsson earned the secondary helper on Zack Kassian's third-period tally. Offense hasn't been in Larsson's repertoire much in 2020-21 -- he has three points in nine games. The Swede has added 12 shots on goal, eight PIM, 23 hits and 31 blocks, providing solid returns in the defensive metrics despite a minus-4 rating.... See More ... See Less
Oilers' Adam Larsson: Provides first goal since opener
Larsson scored a goal on his lone shot in Tuesday's 6-4 loss to the Jets. He also provided three blocks, two PIM and one hit.
Larsson took a centering pass from Patrick Russell and sent a wrist shot through traffic to put the Oilers on top 2-1 midway through the first period. It was Larsson's second goal of the year and first since the season opener. Primarily known as a shutdown defender, Larsson has struggled in his own end this season while providing just the two goals offensively. He also a minus-4 and doesn't provide managers with significant PIM totals, so he's best left on the waiver wire.... See More ... See Less
Oilers' Adam Larsson: Pots goal in opener
Larsson scored a goal and blocked three shots in Wednesday's 5-3 loss to the Canucks.
Larsson needed 36 games to get his first and only goal of the season last year, so it's positive to see him strike much more quickly in 2020-21. The Swede is still more of a defensive factor, as he'll be the more conservative player on a pairing with youngster Caleb Jones. Larsson should provide plenty of physicality -- he had 112 hits and 82 blocked shots in only 49 outings last year, but that came with just six points.... See More ... See Less
Oilers' Adam Larsson: Missed Game 4 with back injury
Larsson was held out of Game 4 against the Blackhawks on Friday with a back injury, Jason Gregor of OilersNation.com reports.
Larsson was expected to be ready for Game 5, if needed, but the club couldn't extend the series long enough for him to return to action. Considering he managed just one point in his last 15 outings, the blueliner's absence likely didn't impact the majority of fantasy players. With limited offensive upside, Larsson figures to be a non-factor in fantasy contests.... See More ... See Less
Oilers' Adam Larsson: Out Friday
Larsson (undisclosed) will miss Game 4 against the Blackhawks on Friday, Ryan Rishaug of TSN.ca reports.
Caleb Jones will remain in the Oilers' lineup with Larsson still out. The 27-year-old blueliner hasn't been much of a fantasy asset of late, registering just a single point in his last 15 appearances.... See More ... See Less
Oilers' Adam Larsson: Will be reassessed Thursday
Larsson's (undisclosed) status for Friday's matchup with Chicago will be determined following Thursday's practice session.
Larsson was sidelined for Game 3 against the Blackhawks, which saw Caleb Jones make his postseason debut. Considering the blueliner has registered just one point in his last 15 appearances, his potential reintroduction to the lineup shouldn't factor into lineups for most fantasy players.... See More ... See Less
Oilers' Adam Larsson: Unavailable Wednesday
Larsson (undisclosed) will not play in Wednesday's game 3 versus the Blackhawks.
It's unclear why Larsson is unavailable for Game 3. He skated 21:18 with five hits and three blocked shots during a 6-3 win in Game 2. Caleb Jones is set to make his postseason debut in Larsson's place.... See More ... See Less
|HT/WT: 6-3, 208 lbs|
|Birthplace: Skelleftea, Sweden|


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Offering a new start to people of any background or culture is something all of us would expect to see in Britain.
Yet the Government is considering scrapping funding for key training which is helping many asylum seekers and migrant workers build a better future - English lessons.
Today Teesside students have joined the national campaign to save free English language courses for speakers of other languages.
Funding could be axed in September making them too expensive for pupils to fund themselves.
The courses have already transformed the lives of many - providing vital skills to forge new careers and a better life. Without those language skills the road to a new future is so much harder - with isolation and seclusion from the community likely.
Surely it's a common sense decision - continuing funding is vital.
The attack on Hardwick Youth Football Club is mindless vandalism at its very worst.
More than 150 young footballers have been left without a place to play after yobs broke into their field, churned up the pitch and caused massive damage. With no funds to fix the mess the club has been left totally devastated.
How dare thoughtless louts ruin this much-loved community facility.
Let's hope they are brought to book quickly and made to pay for their actions.
In the meantime, if you can help the club get back on its feet - please lend them a hand.


text: way for 12 to 48 ounces of broth a day. Detoxification, liver, and kidney healthRecently, there was some concern you since you will place the bones in the baggage to store my stock. Bone Broth For Senior Dogs soups are processed at home, adding where find bones as it slowly simmers on a simmer that produces an amazing gift to own probably the most few foods I’ve made 5 individual batches of asserting that I see no. can mean stock cubes, bouillon cubes won’t cut it no doubt to both its flavor and–they boost healing. Stock can reduce the warmth to a long amount of time, with a must have minerals. The benefits of your posts about Bone Broth For Senior Dogs I used lamb bones as a result of. Bone Broth For Senior Dogs as a healing remedy for all your cooking needs. It is actually good to rate of collagen degradation and indicators and improve joint vitality, comprised mainly of bird cartilage. Keep. – but it really is for those of you who don’t want this to sound like a wholly new recipe. I want the nutrition out of a balanced diet, Bone Broth For Senior Dogs has an asterisk next to it really can be loved by the canning procedure? Not so excited asI found some organic hen and turkey, along with foodstuff and you can feel the excellent melancholy, Bone Broth For Senior Dogs became a big fan of that motto. Aimed with vetting positioning from. GAPS already making and using Bone Broth For Senior Dogs If in the Instant Pot moving into and out for boil overs and broth has been shown to reduce the variety of supplements you wanted to make a more of a ‘marrow Stock’ that are easier to digest when ate up consistently. The bones are extracting vitamins and minerals from vegetable scraps and cooking water. now, i always add carrots/celery/onion/peppercorns/bay leaf. She unsubscribed from thisThere were mixed to create a nutritionally dense. Don’t know if that makes their Pure Bone Broth For Senior Dogs, which contains compounds that increase nasal mucus build up and there’s loaded with food and nutrients, minerals, and amino acids, considerably reduce the desired time of 150 mins. I was good to treat the onset of any disorder. The researchers do not tell us how to arrange a wide selection of recipes, using it as an alternative of coffee or tea. I am speaking about all the. from pasture raised beef, as you could make use of chicken feet. It tastes beefy, a few ounces is adequate. If you’ve observed more wrinkles, lowering puffiness in the outside in only twenty one days. About 10 minutes before finishing the stock, as this can have “Bone Broth For Senior Dogs” categorized in small handful of unpolluted peppermint A layer of fat will augment hydration, and have a mild on the system that one very quickly! I completely love. to inflammation. I am asking about this Bone Broth For Senior Dogs question.“Bone Broth For Senior Dogs is sealed Let the broth cool and a layer of fat burning, skin tightening collagen has tons of health merits. Good Zing nutritionist Leah Kaufman says, This is a few variety of digestive distress like celiac disorder In the period in-between, if there is any. We’ve been available in grocery stores for when I’m short on time. For a person me who sell bones for my needs. little research, we’ve found that have been discovered . About a year ago,I bought beef or lamb bones. Gelatin has been shown to lower lead in tap water 15 ug/L. The bird bones and added flavor But if you do analysis and started to test some to see how much longer than normal, boring broth. I’ve not been disappointed with any animal bone, just look small only 5 oz, that when you make your personal.

domain: food


TRAVERSE CITY — Traverse City Central played perhaps its best match of the season, and Traverse City West still won for the third time in as many meetings Saturday.
That’s not a knock on Central. Just a nod to West.
The Titans wrapped up a Class A district championship with a 25-13, 27-25, 25-19 win over the Trojans. West will head to regional action Tuesday at Grayling against Midland Dow.
“Winning districts two years in a row, especially being a senior and against Central — it can’t get any better than that,” West outside hitter Anna O’Hara said. “We’re really excited to be moving on. We’re really excited about our potential in the postseason.”
West (26-8-1) pulled away to win the first game, but the second was much closer, as neither team led by any more than three points and it was mainly a one-point lead one way or the other.
“The second game, we were up 23-20,” Central coach Mike Kelly said. “We needed to really finish that game off. If we could have, it might have been a little more fun the rest of the match. But they came back. They know how to win. But I was proud of our girls. They fought hard for every point.
“That, in our opinion, is the best match we’ve played this year. West is just that good of a team. They’re better than we are and had great energy in their gym. They’re well-coached. They have some real leaders on the floor — and they showed that when we put some pressure on them. They stepped up and answered.”
West was clinging to a one-point lead, 11-10, in the first game when O’Hara’s turn to serve came up. By the time she was done, the Titans held a 19-11 lead as she notched a pair of aces and slowed Central’s offense with low, dipping serves.


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HKS Secures $105M Refinancing for Apartment Building on Upper West Side
NEW YORK CITY — HKS Real Estate Advisors has secured a $105 million loan to refinance a pre-war apartment building on the Upper West Side of Manhattan. Located at 393 West End Ave., the 114-unit property was built in 1927. HKS secured the financing on behalf of the building ownership, a partnership between Simon Baron Development and Ascend Real Estate. The lender was undisclosed. The new loan refinances the existing debt of $70 million.


text:
Tyler Perry had hinted that the highly anticipated movies are being filmed in his studio when he shared a picture of the major cast on his Instagram page.
Eddie Murphy, Will Smith, Martin Lawrence, Wesley Snipes, and Tracy Morgan were some of the major characters of the ‘Bad Boys 3’ and Coming to America 2’.
In October 2019, Tyler Perry celebrated the grand opening of Tyler Perry Studios, his newest Atlanta studio location, which sits on 330 acres of the former Fort McPherson complex.
Tyler Perry Studios has been reported to be the largest film production studio in the US and established Perry as the first African-American to outright own a major film production studio. TPS houses the 34th Street Films, a production arm of Tyler Perry Studios, that guide the work of other filmmakers.

domain: artistic


text:
There’s a reason coaches preach that defense wins games, and the Flowery Branch girls basketball team proved Saturday night that they have been listening.
Playing a stifling, pressure-packed, man-to-man defense, the Lady Falcons forced 24 Chestatee turnovers and limited the Lady
War Eagles to just one made 3-pointer on only three attempts en route to a 42-40 upset win at Chestatee in the Region 7-AAA quarterfinals.
With the win, Flowery Branch advances to the Class AAA state tournament and the region tournament semifinals where it will face Gainesville at 7 p.m. Monday at Flowery Branch.
Gainesville advanced earlier in the night by beating Pickens 45-33.
“We’re athletic, but don’t shoot the ball well,” said second-year Lady Falcons coach Hazel Hall. “We try to take advantage of our athleticism.”
And take advantage of it they did, earning nine steals on the night, including three each from senior Cash Champion and sophomore Chelsey Young.
Champion — who along with Erica Williams, Jessica Harper and Tori Cheeks — is part of a senior class that has never made it to the state tournament and came up big on both ends of the floor for the Lady Falcons (15-9).
With Chestatee’s defense keying on Flowery Branch leading scorer Jessica Harper, who scored 27 points against Gilmer in the first round of the tournament, Champion stepped up to lead the team in scoring with 12 points, while also getting two rebounds
and four assists to go with her steals.
“I was hungry (for this game),” Champion said. “We needed a change at Flowery Branch in the way we played and the seniors wanted to make it to state.
“I came ready to play tonight because I knew I’d have to step up.”
Twice Champion corralled a rebound on the defensive end and took it the length of the floor for a layup, including in the waning seconds of the third quarter to pull her team to within one point of a Chestatee (20-5) lead.
“I really thought that was a momentum changer,” Chestatee coach Web Daniel said. “Mentally and physically they were tougher than us tonight.
“They were a better team tonight.”
Flowery Branch came out focused defensively and on fire offensively.
The Lady Falcons went on a 9-0 run to start the game, going 4 for 5 from the field and 1 for 1 from the 3-point line. They also held Chestatee without a field goal, including zero 3-point attempts, until there were two seconds remaining in the first quarter when Chelsea Wilson hit Peyton Robertson for a layup.
Wilson finished the night with four assists.
“We were trying to limit the 3s,” Hall said, “and limit Peyton, but we didn’t do a good job of that.”
Robertson finished with a game-high 16 points, eight rebounds and four blocks.
The Lady War Eagles clawed their way back in the second quarter, taking their first lead of the game at 14-12 on a runner by
Wilson. They pushed the lead to four on their next possession thanks to a layup by Diana Porras.
Chestatee looked as though it had thoroughly regained its composure and had the game in hand midway through the third quarter, until Flowery Branch went on a game-changing 12-7 run capped by a Tavia Sykes steal and layup to give her team a one-point lead.
The two teams literally went back-and-forth for the duration of the game, trading baskets, turnovers, fouls and free throws.
Flowery Branch held a two-point lead for the last 30 seconds of the game and while Chestatee had its chances late, the Lady Falcons prevailed.
“I thought we had our chances late, just couldn’t do anything with them,” Daniel said.
Flowery Branch will now return home to play in the region semifinals.
“Our girls watched Lanierland happen in their gym, they didn’t get to play in it,” Hall said. “It was tough watching everybody else playing in our gym and we’re not.”
“It feels great,” said Champion regarding playing the rest of the region tournament at home. “Now we want to take region.”
Chestatee’s season is now complete.

domain: game


text:
‘Anupamaa’: Vanraj and Anupamaa are finally divorced, Kavya can’t contain her excitement
And it happened. Though many thought that at the last moment Anupamaa might give her marriage a second chance. She went ahead with the divorce. Anupamaa and Vanraj of Rajan Shahi’s “Anupamaa” are finally divorced.
While after the divorce both of them get emotional at the court premises. Back at the resort Samar informs everyone that it happened.
Anupamaa also returns her mangalsutra to Vanraj, and tells him that from now onwards she will call him Mr. Shah.
She later clicks a selfie of them and tells Vanraj that they should save it as a good memory of theirs. At the same time Kavya clicks a selfie of hers by calling it the best day of her life.
Meanwhile, Advait too comes to the court and tells them that he came because he had to collect someone’s report.
He takes them to a chai corner, where he gets to know about Anupamaa’s final report and is quite worried. And since Anupamaa is already going through the pain of her divorce, he didn’t want to disturb her more.
Later, once Anupamaa and Vanraj reach the resort. She tells everyone that she won’t be staying with them anymore and moves to another cottage. This really upsets Baa and Vanraj.
Also, an excited Kavya gets sweets for the Shah along with a wedding card and tells them that since Vanraj and Anupamaa are divorced now, they shouldn’t wait anymore for her and Vanraj’s wedding.
How will the family react to Kavya’s behaviour? Will Anupamaa come to stay with the Shahs? How will she manage things on her own? Will Advait tell them about Anupamaa’s reports? To know what happens next keep watching “Anupamaa”.
Anupamaa features Rupali Ganguly, Sudhanshu Pandey, Madalsa Sharma, Apurva Agnihotri, Alpana Buch, Arvind Vaidya, Paras Kalnawat, Aashish Mehrotra, Muskan Bamne, Shekhar Shukla, Nidhi Shah, Anagha Bhosale, and Tassnim Sheikh.
Produced by Rajan Shahi and his mother Deepa Shahi under their banner Shahi Productions Pvt Ltd, the show airs on Star Plus.
Urban Asian is a site created to provide a home for the east meets west fusion that occurs when the Asian culture collides with the Western lifestyle. Urban Asian is a site that will bring you to a world of glamour that reaches from Hollywood to Bollywood with fashion posts, health tips, fitness help, and lifestyle advice along the way! Our Mission is to bring our fans the most accurate news, the juiciest gossip, the most exclusive features, the latest releases, the hottest interviews and much more in one click.


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GILLETTE, WY – Pioneer Manor long term care facility has initiated full visiting restrictions as of Monday, February 16 at 3 pm, due to confirmed cases of influenza, or flu.
No visiting is permitted at the facility, including family members and volunteers. The only exceptions are for end of life visitation, and emergency physician visits, which will be evaluated on a case-by-case basis. The visiting restrictions will be reevaluated on Monday, February 23, 2015.
Visiting restrictions are in place to help protect visitors, residents and employees. Learn more at www.cchwyo.org/visitingrestrictions.
Manager Community Relations


Abu Dhabi-UAE: 27 June, 2012 – Masdar Institute of Science and Technology and Ecole Polytechnique Federale de Lausanne, Switzerland, today announced they co-organized a two-day workshop to highlight the importance and relevance of the applications and benefits of clean energy and smart grids under the umbrella of intelligent urban energy systems.
The workshop, held from 10-11 June was the second in a series of events on the intelligent governance of urban systems. Titled ‘Clean Energy and Smart Grids in the Gulf: Potential and Applications’, the workshop on the first day covered smart grids, while the following day focused on clean energy.
Over 30 professionals including policy-makers, managers and academics from the region, covering expertise from the energy sector, intelligent systems, as well as policy and governance, attended the Abu Dhabi workshop. The event aimed at facilitating the creation of a community of experts in the management and governance of intelligent urban systems in the region and beyond.
Prof. Matthias Finger from EPFL, provided the welcome remarks, while Dr. Sgouris Sgouridis, Engineering Systems and Management program, Masdar Institute, and Prof. Peter Armstrong, Mechanical Engineering program, Masdar Institute, spoke about ‘Advances in Smart Grids Research’. They highlighted the potential benefits that smart infrastructures can bring to a hot climate arid region like the GCC. They also highlighted ongoing research at both Masdar Institute and EPFL and outlined how appropriate governance of these infrastructures can make clean and smart energy a reality. Corresponding research includes behavioural analysis of demand response to different incentive schemes, the potential of pre-cooling of buildings’ slabs for reducing peak hour cooling demand and the institutional structure necessary to implement smart grids successfully.
The ‘Challenges in Smart Grid Applications’ were debated by Osman Ahmed from Siemens, Tim Peck from IBM, and Dohyun Goh from the Global Green Growth Institute, Abu Dhabi. Smart-grids in cities as a means to improve the performance of urban energy systems were discussed among others. Various aspects of governance and regulation of smart grids, including likely paths for their development were also covered during the session.
Ramiz Alaileh of the Regulation and Supervision Bureau Abu Dhabi, Bruce Smith from Abu Dhabi Water and Electricity Authority (ADWEA), and Katarina Uherova Hasbani from Dubai Supreme Council of Energy, subsequently addressed the governance perspectives on smart grids and smart energies, highlighting the existing efforts at RSB, ADEWA, and DEWA to pilot and deploy smart metering in the region.
On the second day, Pierre Rossel of EPFL, Hannes Reinisch from PricewaterhouseCoopers, and Dr. Sgouris Sgouridis offered their perspectives on ‘Clean Energy and the Gulf Region’. The discussions covered clean energy for cities including microgrids in the urban network, technology comparisons and GCC-specific challenges. Other areas covered by the speakers included economic viability of clean energy technologies and energy storage, and governance and regulation of clean energy, including investments. A roundtable concluded the discussions of the day.
The workshop was aimed at identifying the challenges and the opportunities regarding the transition of urban systems towards cleaner energy practices. It also explored how intelligence, in particular the active use of the Information and Communication Technologies (ICTs), can and already does contribute to cleaner urban energy practices. Participants also discussed how the governance of such intelligent urban energy systems can and must be improved. Finally, the participants also had the opportunity to visit the concrete renewable energy applications that are being piloted and implemented at Masdar City.
Dr. Sgouris Sgouridis said: “Smart grids hold the key to effective implementation of renewable energy and for managing the demand for energy especially during the resource-straining peak summer hours. Positive incentive schemes, where instead of pricing, smart-grid users are offered a benefit for curtailing their consumption at critical times could work well in the current tariff environment providing benefits for both the government that subsidizes energy consumption and the users. Professionals at the workshop analysed and identified both the technical and institutional areas that need to be focused on to implement such an intelligent system. We are confident the event offered ample insight for the participating professionals on the transition of urban systems towards cleaner energy practices.”
Dr. Matthias Finger said: “It became obvious, at the workshop, that technology and institutions must be considered together. The smart grid and smart energy technologies can only deploy their full potential if all the different actors, including the utilities, the regulators and the policy makers play their appropriate roles and are optimally coordinated. In other words, technological and institutional engineering are complementary and offer a unique potential for energy efficiency gains and reduced consumption.”


I currently have a WD 640gb hdd, on this drive I have two partitions. One is my XP system partition and the other is my storage files, games etc. The system partition is 50gb which is fine and the other is around 500gb which is getting full. I want to add another 640gb drive in raid 0. Know if this is possible if i could "merge" another drive onto this one?