text:
Beach front land on Koh Sirey. This land is located on the island of Koh Sirey,which is linked to Phuket by a bridge. 13 rai with 80m of beach frontage. Very nice a quite place, yoг can built up there treatment hotel, condominium or villas.
|Sea distance||0.00 km|
|Plot area||20800.00 m2|


text:
Advance Medical Directives
The Virginia Health Care Decisions Act authorizes any competent adult (principal) to make an advance medical directive, which incorporates both a living will (a written declaration which allows you to state your wishes regarding the use of life-prolonging medical care if you are terminally ill) and a medical power of attorney (which allows you to appoint another person to make any or all health care decisions for you if you are incapacitated).
A person is considered incapacitated if he or she suffers from a mental and/or physical disorder which “preclude[s] communication or impair[s] judgment as a result of the inability of the patient to make an informed decision” about a particular medical treatment because he or she is “unable to understand the nature, extent or probable consequences of the proposed medical decision, or to make a rational evaluation of the risks and benefits of alternatives to that decision.” The incapacity determination must be made by the principal’s attending physician and a second licensed capacity reviewer, who is not involved in the principal’s care, after a personal examination of the principal.
The agent must abide by the directions stated in the advance medical directive. When the principal has not stated whether or not he or she would want a specific treatment, the agent is authorized to make a decision based upon what the agent knows about the principal’s beliefs and what the physicians consider medically appropriate. An advance directive in Virginia may be revoked in writing, orally or by destruction of the document by the declarant.
There is one important caveat to the authority of the agent regarding mental health care. The only time an agent can authorize mental health treatment, or even admit the principal to a mental health facility over the principal’s protests, is if the attending physician thinks it is medically appropriate and the principal included an express statement in the advance medical directive that such treatment be administered even if he or she protests. In that instance, the advance medical directive must also contain a physician’s signature attesting that the principal understood the provision and was capable of making an informed decision at the time the advance medical directive was created. It may be appropriate for a principal with a history of mental health issues to include such a provision.
It is important to note that A Do Not Resuscitate Order (“DNR”) is completely separate from an advance medical directive, and with a much different purpose. A DNR is an order written by a physician which directs that cardiopulmonary resuscitation be withheld in the event of cardiac or respiratory arrest. Legislation passed in 2010 added licensed health care practitioners at registered continuing care communities to the list of those authorized to follow DNRs.

domain: medical


text:
A new biography chronicles the life and charitable activities of Tracy W. McGregor (1869-1936), who established the McGregor Fund, one of Michigan's first major foundations. The volume chronicles McGregor's civic leadership in Detroit, describes his efforts to support the homeless, and explores his interest in books and libraries. McGregor's activities spanned periods of industrial growth in Detroit, as well as economic decline, as workers began to experience the effects of the Great Depression.
Professor of history Philip P. Mason examined the archives at Wayne State University and other institutions for this detailed biography. Click here for a brief profile of McGregor and a listing of the materials that can be found in the archives.
Humanitarian, Philanthropist, and Detroit Civic Leader and other biographical works in our collection can be found by searching the Catalog of Nonprofit Literature.

domain: library


Meeting for new Perche Creek Trail set for tonight
COLUMBIA — Columbia Parks & Recreation will host a public input meeting tonight to discuss plans for a new trail at Perche Creek.
"The proposed Perche Creek Trail will be about eight or nine miles long along the Western side of Columbia," park development superintendent Mike Snyder said. "This is the first phase of that. It'll be about a mile in length."
The first phase will connect an abandoned road off of Gillespie Road, run south along Perche Creek and connect with the MKT trail. The trail will be made of concrete and include a bridge that runs over Perche Creek. The budget for the project is $1.2 million.
"The earliest we could get started on work would be late fall or spring of 2021," Snyder said. "That'll probably take around, oh, six months for construction."
The proposal has been on the trail master plan for over 20 years.
"It's been conceptually planned for a long time," Snyder said. "As far as actually the construction drawings and the detail planning that's going into the exact route we have right now, that's been ongoing for, oh, a little over a year now."
58-year-old Scott Mullins lives off of Gillespie Road and enjoys riding his bike with his dogs in his free time. The new trail proposal will make for an easier commute to a bike trail.
"We take that spur that goes along Perche Creek and crosses Scott Boulevard, you know. That's our access points now," Mullins said. "This will make it a lot more handy for us really to get right on the trail."
The trail is part of a future plan for a 30-mile loop that will go around the city of Columbia.
"This 30-mile loop that's proposed around Columbia is one of the things that really could put Columbia on the map as a trail destination community," Snyder said.
The public input meeting will take place at Jay Dix Station from 5 p.m. to 7 p.m. People like Mullins are encouraged to give suggestions for things to be included in the first phase of the new trail.
Mullins is concerned there won't be a place to park where the proposed trail ends on Gillespie Road.
"My other concern is that the trail may not be as accessible if there's not a parking area for people to stop and, you know, get their bikes ready and stuff," Mullins said. "I may go just to give a little input."
For people who were unable to attend the meeting, they can still give suggestion through an online survey by July 15.


text:
By Andrew Bishop Mkandawire
The silence that clouded parents from opening with their youth on matters of sexual reproductive health in Lilongwe and Kasungu district supporting implementation Family Planning Association of Malawi (FPAM) WISH2ACTION project continues to break with the coming of video and audio cards that are parked with family planning interactive and educative messages. Chiefs, health workers and parents have all agreed that the devices are friendly to both parents and youth as they carry health information necessary for all of them.
Senior Chief Kapelura in Kasungu said “Before WISH2ACTION project, we lost many girls and women from unsafe abortion complications due to unplanned pregnancies and those cases have completely dropped because parents are no longer resistant to permitting their wards access health services like family planning at mobile clinics. These video cards will even push us to reach more youth with contraception information and eventually managing successful referrals.” SGHV M’biya from Kasungu learnt that her area is already geared to serving youth, men and women including people with disabilities from accessing family planning services because she knows that responsible community members start with good health. “The digital players will just cement what we already do,” sha added. Block leader for Nsungwi Area 25A in Lilongwe concurred with SGVH Kauma of Area 12 and Kawale in Lilongwe that sometimes parents require intellectual approach to reach out to youth and such devices can just boost that confidence.
Kasungu and Lilongwe district hospital offices have showed that the cards will help dispel myths and misconceptions and back up the community health workers with accurate, referential and trusted information for communities. “Kasungu district is one of the districts that are infested with myths and misconceptions regarding family planning services. Let’s use the messages contained in the digital players to dispel those lies by allowing community members watch and listen to the well-crafted family planning messages,” Alufeyo Chirwa, Family Planning Coordinator for Kasungu district hospital. And Lilongwe Deputy Family Planning Coordinator Jean Kayamba encouraged parents and guardians to engage youth with family planning conversations because the rate of youth felling for unplanned pregnancies in Lilongwe is high.
Community Health Workers have also cerebrated the coming of digital cards because they are attractive and interactive. “These cards have upgraded our health education job description and we will be accepted in communities with a fresh touch,” said Crissy Kumvula, Senior Disease Control and Surveillance Assistant (SDCSA) at Area 25 Health Center in Lilongwe. In Kasungu, FPAM community health worker Innocent Folopezi asked chiefs reach more youth because most youth look up to chiefs for guidance and information.
Parents who volunteers to engage youth with disabilities to access basic education, care and support attended Kawale family planning dioalogue meeting where they also received the digital players to support their job of education youth with disabilities to eguip them with health information. Ethel Kapakasa is a MACOHA volunteer and said “my job requires a lot of support because with my frisnds we teach and care for people with disabilties and today we have received video and audio cards that will support health education session for youth with disabilties.” Mpakasa added that “As the youth grow up, their sexual desires become evident and they need to learn how to protect themselves from unplanned pregancies and sexually transmitted infections.”
FPAM Director of Programmes, Tazirwa Chipeta commented that FPAM is geared to come up with more innovative ways that can accelerate dissemination of correct and accurate sexual reproductive health education and information to people of reproductive age. “Girls and women are encouraged to use our Digital Health platforms including USSD, Mobile Media – Video and Audio Cards, chatbots Facebook and WhatsApp chatbots,” she added.
The video and audio cards are addressing benefits of delaying first pregnancy, birth spacing, male engagement, minimizing stigma and discrimination, and dispelling myths and misconceptions regarding community access to family planning, targeted WISH2ACTIOn project implementation areas of Kasungu and Lilongwe. The project aims at promoting leave no one behind when it comes to community access to sexual reproductive health services to communities living in hard-to-reach areas.

domain: news


text:
Where: Mingo Falls, near Cherokee
Activity: Short hike
Distance: 0.2 miles (0.4 round trip)
Mingo Falls is a 120 foot waterfall, just 15 minutes north of Cherokee off of Big Cove Rd.
Although the trail says the falls are 0.4 miles from the trailhead, the falls were actually only about 0.2 miles from the small parking lot, making the round trip just under 0.5 miles if you don’t explore around the falls.
As you can see in the picture above, the first several hundred feet are stairs. This is why the trail is considered “moderate difficulty” according to some sources, but take your time if you need it, the view is worth it. There are a few park benches along the route.
Once you see the bridge, the falls are just around the corner.
The wide wooden bridge offers an excellent viewing platform of Mingo Falls.
There are a few worn down unofficial trails from people who have been exploring around the falls, but they are steep and can be very slick. However, the official trail ends here at the bridge.

domain: geography


text:
Cicilline weighs in on Mueller report, saying impeachment is on the table
PAWTUCKET, R.I. (WLNE) — Rep. David Cicilline said Friday morning that impeachment is on the table when it comes to possible action by Congress against President Donald Trump. Cicilline spoke to reporters at his Pawtucket office about the Mueller report, and the next steps Democrats may take.
Cicilline called the report “damning” for the President, despite Mueller’s finding there was insufficient evidence to prove Mr. Trump committed a crime. Cicilline is a member of the House Judiciary Committee, which issued a subpoena Friday asking for the unredacted report.
Rep. Cicilline said he is especially concerned by what he calls the President’s encouragement of Russian efforts to interfere in the 2016 election, and attempts to obstruct the investigation into that.
Cicilline hopes to see swift action by the House Judiciary Committee to further investigate the President. Attorney General William Barr will testify before the committee on May 2, and Cicilline hopes to have Mueller himself testify shortly after that, to walk lawmakers through the report.
As for impeachment, Cicilline says if that’s where the investigation leads, so be it.
“I think it has to weigh very heavily on us, if at the conclusion of our investigation we conclude that the president engaged in high crimes and misdemeanors. I think the constitution requires us to move forward. I don’t think we have the luxury of saying I don’t want to do this because it’s bad for us politically,” said Cicilline. “I think the Speaker has said we should never do impeachment for a political reason. We should never refuse to do impeachment for a political reason.”
Cicilline also called Barr’s conduct “outrageous,” accusing him of providing a misleading summary of the Mueller report, and using a press conference to further spin what’s actually in it.
© WLNE-TV / ABC6 2019

domain: law


plastic crusher equipment price in pakistanFeb 13, 2016 . From large primary jaw crusher and impact crusher to cone crusher and Pakistan Plastic Crusher Machine, TENIC Machinery Pakistan Plastic Crusher Machine plastic crushing machine pakistan; Brick Crushing Machine, plastic crusher equipment price in pakistan exports. boil crusher machine price in.boil crusher machine prices in pakistan,stone crush machine pakistan price - tfgstone crussing machines in pakistan - stcorporation. stone crushing machine price in pakistan simpulindonesia. Stone Crushing Machines Prices In Pakistan. What is the price of stone crusher? Quora. Get More Info. image.
Comments About boil crusher machine prices in pakistan
Stone & Jaw Crushing Machine - Stationery Crusher Manufacturer .
We introduce ourselves as one of the leading manufacturers of Crushing Machines, which are used in various sectors. These products are available in various specifications and at market leading prices. Range of our crushing machines includes Crushing and Screening Stationary, Crushing and Screening Portable, Roll.
Sugarcane Juice Machine - Manufacturers, Suppliers & Traders
Find here details of companies selling Sugarcane Juice Machine, for your purchase requirements. Get latest info on Sugarcane Juice Machine, suppliers, manufacturers, wholesalers, traders with Sugarcane Juice Machine prices for buying.
screw mill machine manufacturers in pakistan - small rock crushers .
conveyor belt manufacturer in pakistan - crusherasia. Boil Crusher Pakistan Manufacturer; . Screw Conveyor and Bucket Elevator offered by Transpower, . Grinding Mill News. Machine News. Gold News. Feed News. Get Price.
Kitchen Appliances Prices in Pakistan | Buy Kitchen Appliances .
iShopping.pk has considerable collections of branded Kitchen Appliances at lowest prices in Pakistan with having free delivery extensively at any place to your door step.
Crushing Machine - Cone Crusher Manufacturer from Coimbatore
Manufacturer of Crushing Machine - Cone Crusher, Horizontal Shaft Impact Crusher, Primary Jaw Crusher and Stone Crushing Machine offered by Geco Grinding Centre, . Approx. Price: Rs 9.5 Lakh / Piece .. We are the leading manufacturer of Hydraulic Stone Crushing Machine and our product is made of good quality.
plastic crusher equipment price in pakistan
Feb 13, 2016 . From large primary jaw crusher and impact crusher to cone crusher and Pakistan Plastic Crusher Machine, TENIC Machinery Pakistan Plastic Crusher Machine plastic crushing machine pakistan; Brick Crushing Machine, plastic crusher equipment price in pakistan exports. boil crusher machine price in.
stone crush machine pakistan price - tfg
stone crussing machines in pakistan - stcorporation. stone crushing machine price in pakistan simpulindonesia. Stone Crushing Machines Prices In Pakistan. What is the price of stone crusher? Quora. Get More Info. image.
boil crusher machine prices in pakistan,
Briquette Crushing Machine - Manufacturer from Gurgaon
Manufacturer of Briquette Crushing Machine offered by Pals & Company, Gurgaon, Haryana.
Bricks Crusher Machine - Manufacturer from Ahmedabad
Manufacturer of Bricks Crusher Machine - Brick Crusher, Brick Crushing Machine offered by Mix Well Hardic Engineering, Ahmedabad, Gujarat.
Walnut Crushing Machine - Alibaba
Alibaba offers 1256 walnut crushing machine products. About 48% of these are other food processing machinery, 4% are crusher, and 1% are plastic crushing machines. A wide variety of walnut crushing machine options are available to you, such as grain processing equipment, cooking equipment.
DEP Agro Machineries Private Limited- Leading manufacturer and exporter of sugarcane crusher, heavy duty sugarcane crusher, agriculture sugarcane crusher, bullock driven sugarcane juice machine, agro sugarcane crusher, agriculture heavy duty sugarcane crusher, industrial agriculture sugarcane crusher, agriculture.
Crushing Plant - Jaw Crusher Manufacturer from Guwahati
Portable crusher plant is versatile to all portable smashing needs, portable crusher plant sets up another scope of business open doors for contractual workers, quarry administrators, reusing and mining applications. The versatile crusher plant for stone pulverizing highlights low transportation cost, adaptable design, simple.
Pellet Making Machine - Rajkumar Agro Engineers Pvt Ltd
Manufacturer of Pellet Making Machine - Animal Feed Pellets Making Machine, Poultry Feed Pellets Making Machine, Rabbit Feed Pellets Making Machine and Floating Fish Feed Pellet Making Machine . Rajkumar Corn Grits Machine performs one time cleaning, peeling, crushing and produces corn grits of different sizes.
Sugar Production from Cane Sugar (Practical Action Technical Brief .
Feb 1, 2018 . Syrup and lump sugars can be produced using relatively basic low-cost equipment consisting of a crusher and a furnace with boiling pan. Granular sugar production also uses crushers and boiling equipment but requires additional equipment such as a clarification plant, crystallisers, driers and packaging.
Jaggery Manufacturing Unit|Jaggery Unit Investment/Machines
Dec 21, 2012 . Jaggery Manufacturing Unit | Jaggery Preparation using Machines | Jaggery Unit Investment . the production and crushing of Suger Cane and stood third position in Production of Jaggery. . Jaggery is prepared by boiling the sugarcane juice at high temperature till the water level is evaporated and the.
Cane Crusher Machine - The Adarsh Engineering Works
we are the leading manufacturer and supplier of cane crusher machine - 7.5 hp, sugar cane crusher machine - 10 hp, Mild steel crusher and steel gears crusher . Ask for Price. Item Code: RASRAJ-3. Sugarcane Crusher Machine – 10 HP is designed on sound engineering principles. Our crusher machine is powered by a.
boil crusher machine prices in pakistan,
Sugar Plant Machinery - Boiling House Equipment Manufacturer .
Manufacturer of Sugar Plant Machinery - Boiling House Equipment, Cane Crushing Mill, Sugar Plant and Rotary Vacuum Filter offered by Kay Iron Works (Yamuna Nagar) Private Limited, Yamunanagar, Haryana.
Anex Home & Kitchen Appliances Online Shop in Pakistan .
. market in Pakistan. Anex deals in grinder, juicer, blender, chopper, fryer, food factory, ice cream maker, toaster ovens, steamers, sandwich maker, roti maker, cooking appliances, pizza maker, coffee makers, espresso makers, popcorn maker, cotton candy machine, food factory and washing machines etc. You can buy all.
Food Processing Machines - Khoya Making Machine Manufacturer .
Manufacturer of Food Processing Machines - Khoya Making Machine, Heavy Duty Mixer, Lassi Making Machine and Sugar Candy Machine offered by Sujata Electricals, Pune, Maharashtra. . Besides these, customers can avail product from us in accurate dimensions and at affordable rates. .. Fruit Mill - Fruit Crushers Mill.
To ensure complete satisfaction to our customers, we have developed an innovative design for this crushing equipment so that it can function in a smooth way. . with S.S. & Mild steel materials which ensure its features like highly efficient, resistance to corrosion, robust construction, easy installation and low investment cost.
Crushing Machines - Pharma Scrap Crusher Exporter from Mumbai


LIMA, Peru (AP) – A tanker truck carrying natural gas exploded in a major street in Lima’s Villa El Salvador district on Thursday, killing at least two people.
The explosion, which also set fire to several buildings and cars, also saw 48 people injured.
Fire department chief said the explosion had sparked from a leak in the truck.
14 homes were burned in the fire, officials said.
Official information indicated an adult died next to the truck and has not been identified, while a child died after being taken to hospital.
According to Peru’s health minister eight children were among the victims.


Wolverhampton Wanderers sealed a Premier League double over West Midlands rivals Aston Villa with a 2-1 win this afternoon.
The win cements bragging rights for the Molineux faithful in his particular rivalry and moves the Old Gold up to seventh place overnight.
Bruno Lage will be impressed with his side after they bounced back from the disappointing 3-2 defeat against Leeds United just before the international break, with Jonny Otto opening the scoring and an Ashley Young own goal ultimately giving Wolves the three points.
Although the victory means that they have now won three of their last four games, questions over why 19-year-old Fabio Silva has not featured more this season may become pertinent.
At such a young age, the Portuguese starlet has “enormous potential” according to Daily Mail senior sports reporter Ollie Lewis, and although his underlying stats do not flatter him, he put in a spectacular performance for Wolves this afternoon.
Whilst his hold-up play demonstrated maturity and his physicality caused issues among the Villa defence, Silva’s ability to create space for team-mates was reminiscent of an old-fashioned centre-forward from yesteryear, as he grafted throughout a display which more than repaid Bruno Lage’s faith in him in what was just his third Premier League start this season.
The former Porto prospect has struggled to get to grips with the English game, and those who solely rely on statistics would have believed that the 19-year-old capitulated today. Although the young striker had fewer touches than both goalkeepers and completed just 12 passes, the fewest of any Wolves starter, it was a performance which could only be valued through the ‘eye test’.
Writing in his post-match player ratings for Birmingham Live, Alex Dicken awarded the teenager an eight, with the summary of: “Other than a first goal of the season, there wasn’t a great deal missing from Silva’s display. In just his third Premier League start of the season, Silva showed intelligence and awareness to get the better of Villa’s physically imposing defensive duo. The 19-year-old’s link up play with Trincao and Podence was very good.”
Nonetheless, Silva will have been questioning how his name evaded the scoresheet, with his two big chances providing an xG total of 0.50. Those kind of chances from that specific area of the pitch would certainly go in for him on another day.
At a time when Raul Jimenez is serving his initial two-match ban, the Portuguese youngster has just put in a performance which will surely put him in contention to achieve his first back-to-back Premier League starts under Lage this season when Wolves make the trip to Newcastle next Friday.


text:
First snowfall causes no travel headaches at ELM Airport
BIG FLATS, N.Y. (WENY) - Thursday marked the first snowfall of the season.
Officials at the Elmira Corning Regional airport say they have been preparing for Thursday's conditions. The airport say every year they make extensive plans for snow and ice removal, and have all the right equipment to keep the runways and ramps cleared. Officials say because of this, they can keep up with a pretty significant snowfall, making sure the airport is up and running. WENY spoke to the Director of Aviation, Tom Freeman about Thursday's weather conditions. Freeman says he was not too concerned about the weather affecting any flights.
"Really its what happens in Detroit, or what happens in Washington DC. But for the weather we're expecting here today we shouldn't have any delays," says Freeman.
Temperatures will continue to drop Thursday night into the low 20s.


text:
Viktor Tikhonov scores 2 goals in KHL debut (Photo © khl.ru)
Viktor Tikhonov, who was loaned to Severstal of the KHL by the Phoenix Coyotes, scored two goals in his debut game.
Tikhonov spent on ice 17:56, had 7 shots on goal and a +2 rating. His Severstal faced HC MVD and won 5:3.
“I missed Russia and our hockey very much,” said Tikhonov after the game. “Two goals in a debut is a great start, as for me. I’ll try to keep up the pace. Of course I wanted to score but I didn’t have to try hard because it’s easy to score with such partners as mine. It didn’t take me long to adjust to the game as the team’s roster is almost the same as it was when I left for the North America. My grandfather (famous coach Viktor Vasilievich Tikhonov) was at the game today so I dedicate these goals to him! I will find out if they took the puck for me and then I’ll give it to my grandfather.”

domain: sports


New York, 10 December 2022 (TDI): The United Nations General Assembly (UNGA) adopted Turkmenistan’s proposed resolution during the 45th plenary meeting of the 77th session held on December 6, 2022.
During the meeting, the General Assembly first evaluated Turkmenistan’s draft resolution “International Year of Dialogue as a Guarantee of Peace, 2023” under agenda item 14 “Culture of Peace.”
Presenting the resolution, the Turkmen delegation emphasized that the President of Turkmenistan will present the global initiative; “Dialogue is a Guarantee of Peace” for the first time in December 2021.
It will be presented during the International Conference “Policy of Peace and Trust – the Foundation of International Security, Stability, and Development.”
Moreover, the Turkmen delegation noted that the draft resolution “International Year of Dialogue as a Guarantee of Peace, 2023” was developed following extensive consultations among UN member countries.
It has been drafted after taking into account all constructive opinions and proposals. The document reflects the UN Charter’s fundamental purposes and principles.
Most notably, it reflects the obligation to settle disputes peacefully and the determination to save coming generations from the menace of war.
Likewise, the draft resolution affirms that integrated dialogue, in its various forms, is important in reinforcing collaboration among member states.
Along these lines, the Turkmen delegation, while presenting the draft, highlighted the fact that Turkmenistan will host the International Conference “Dialogue is a Guarantee of Peace” on December 11, this year.
It will do so to provide a forum for a broad exchange of viewpoints on the topics covered in the resolution and lay the groundwork for the launch of the 2023 Global Campaign for Dialogue as a Guarantee of Peace.
Turkmenistan’s delegation invited all United Nations member countries and agencies to participate actively in the upcoming Forum.
It is pertinent to note that this resolution was approved by unanimous agreement following discussions. It was co-sponsored by 68 of the Organization’s member countries.


XK07 - STEM FlatSat Kit
The STEM FlatSat Kit includes xCHIPS to build both a FlatSat (which can be moved in the environment, including being flown), and a Ground Station, to receive and display data.
The FlatSat consist of the following:
- 1 x CW01 - WiFi Core
- 1 x CS11 - Core with SD Card Interface
- 1 x PB04 - Dual AA Intelligent Battery Power Supply
- 1 x RL03 - LoRa Radio (915 MHz)
- 1 x SG33 - VOC & ECO2 Sensor
- 1 x SI01 - IMU 9DoF
- 1 x SL01 - UVA, UVB, Light Sensor
- 1 x SN01 - GNSS (GPS) Sensor
- 1 x SW01 - Advanced Weather Sensor
- 1 x XC10 - 10-Pack xBUS Connectors
The ground station consist of the following:
- 1 x CW01 - WiFi Core
- 1 x IP01 - USB Programming Interface
- 1 x OD01 - OLED Display 128x64
- 1 x RL03 - LoRa Radio (915 MHz)
- 1 x XC10 - 10-Pack ☒BUS Connectors
CW01 - WiFi Core
This xCHIP forms part of the core modules and is equipped with Wi-Fi. The Wi-Fi module offers internet connectivity which allows the user to store data on the cloud which enables remote data access and opens up for the world of IoT.
CS11 - Core with SD Card Interface
This xCHIP is a low-power microcontroller using the 32-bit ARM Cortex -M0+ processor and ranging from 32- to 64-pins with up to 256KB Flash and 32KB of SRAM.
IP01 - USB Programming Interface
This xCHIP is an interface module which is equipped to power and program other modules via a USB A connector. The USB A connection is required for programming the range of CPU Core ☒CHIPS over the USB-serial bridge provided by the FT232R.
OD01 - OLED Display 128x64
This xCHIP forms part of the output modules and is equipped with an OLED display unit that is capable of displaying any graphics or text. Very useful as a sensor data display.
PB04 - Dual AA Intelligent Battery Power Supply
This xCHIP module houses 2 AA batteries to supply power to other connected xCHIPS. The PB04 makes use of the INA199A2 current sensor and MAX11647 Analog to Digital Converter. These two low-cost precision sensors allows for accurately measuring power usage, battery voltage and current draw.
The PB04 features an AAT1217 which is a high efficiency, synchronous, fixed frequency, step-up converter designed for battery-powered applications. The high 1.2 MHz switching frequency and completely integrated control circuitry maintains excellent regulation, ripple, and transient response throughout the full load range.
Light load mode operation and low quiescent current allow the AAT1217 to maintain high efficiency performance for light load conditions. With a 1.2A peak inductor current limit, the AAT1217 is capable of delivering 400mA from dual AA cells batteries.
RL03 - LoRa Radio (915 MHz)
This xCHIP features an SC18IS602B SPI/I2C converter IC for handling radio operations, the on-board RFM95W LoRaTM module provides ultra-long range spread spectrum communication and high interference immunity whilst minimizing current consumption.
SG33 - VOC & ECO2 Sensor
This xCHIP is equipped to sense a variety of Volatile Organic Compounds (VOCs). This xCHIP is based on the CCS811 which is an ultra-low power digital gas sensor which integrates a metal oxide (MOX) gas sensor to detect a wide range of Volatile Organic Compounds (VOCs), for indoor air quality monitoring, a micro-controller unit (MCU), and an I2C interface.
CCS811 is based on ams unique micro-hotplate technology which enables a highly reliable solution for gas sensors, very fast cycle times and a significant reduction in average power consumption. The integrated MCU manages the sensor drive modes and raw sensor data measured while detecting VOCs. The I2C digital interface significantly simplifies the hardware and software design, enabling a faster time to market. CCS811 supports intelligent algorithms to process raw sensor measurements to output a TVOC value or equivalent CO2 (eCO2) levels, where the main cause of VOCs is from humans. CCS811 supports multiple measurement modes that have been optimized for low-power consumption during an active sensor measurement and idle mode extending battery life in portable applications.
SI01 - IMU 9DoF
This xCHIP module comprises 3 sensors, an accelerometer that measures movement of the X,Y and Z axes, a magnometer that computes orientation to magnetic north, on the X,Y and Z axes, therefore we can calculate which direction we are facing as well as the angle at which we are leaning. The last sensor is the gyroscope that measures our orientation to the center of the earth.
SL01 - UVA, UVB, Light Sensor
This xCHIP forms part of the sensor modules and is equipped with a light sensor that is capable of measuring the luminosity (Wide Dynamic Range — 3 lux to 220k lux) (visual brightness), UVA and UVB radiation. The light sensor converts solar UV light intensity to digital data and has displayed reliable performance of UV radiation measurement inner long time solar UV exposure.
SN01 - GNSS (GPS) Sensor
This xCHIP is equipped with a NEO-6M GPS receiver. The 50-channel u-blox 6 positioning engine boasts a Time-To-First-Fix (TTFF) of under 1 second. The dedicated acquisition engine, with 2 million correlators, is capable of massive parallel time/frequency space searches, enabling it to find satellites instantly. Innovative design and technology suppresses jamming sources and mitigates multi path effects, giving the NEO-6 GPS receivers excellent navigation performance even in the most challenging environments.
SW01 - Advanced Weather Sensor
The humidity sensor provides an extremely fast responce time for fast context awareness application and high overall accuracy over a wide temperature range.
The pressure sensor is an absolute barometric pressure sensor with extremely high accuracy and resolution.
The integrated temperature sensor has been optimized for lowest noise and highest resolution. Its output is used for temperature compensation of the pressure and humidity sensors and can also be used for estimation of the ambient temperature.
XC10 - 10-Pack xBUS Connectors


text:
This website is owned by TRAFFIC NETWORK SOLUTIONS, S.L. (hereinafter, The Company), Calle Comandant Benitez 25, local 1 – 08028 Barcelona, Spain – N.I.F. /V.A.T. ESB64902430. Registered in the Company Registry of Barcelona, T 40.643, F. 96, H. B-370.439, I. 1. Use of this website is subject to the following conditions of use. Please read them carefully. Your access to this site and its content means that you have read and agree to these conditions of use. The use of certain spaces or services on this website may be subject to special conditions of use. Please read them carefully. Your use of these services means that you accept these special conditions.
1. CONDITIONS OF USE
The provider, responsible of the website, places this document at the users’ disposal, in order to carry out the obligations set by the EU directive 2000/31/CE and by the Law 34/2002 on Services of the Society of Information and of Electronic Commerce (LSSI-CE), as well as to inform all users about the terms and conditions of the website.
Access to the website and use of its content and services implies full and unreserved adherence to the terms and conditions set forth hereunder, as well as to any other legal regulations that might apply.
The provider reserves the right to modify any information on the website, at any time and without prior notice to users.
This website contains materials that the Company has prepared for information purposes only. These materials may not reflect the most recent updates adopted by the continuously developed products of the company, and we may change, expand upon or update this material without notification.
The links on this website may direct you to other websites or web pages managed by third parties, over which the Company has no control. The Company is not responsible for the contents or the state of these websites and web pages, and access to them via this website does not mean that the Company recommends or approves their content.
2. LIMITATION OF LIABILITY
The provider does not assume any responsibility for the information published or modified by third parties on the website. When browsing the website it is possible to be redirected to contents of websites belonging or managed by third parties. As the provider does not control all the information published on outside websites, it cannot assume any responsibility regarding its contents. However, the provider intends to delete any content that could contravene the national or international legislation, the morality and public order, by removing the link to the outside website, and warning the corresponding authority on such content.
The provider does not assume any responsibility for the stored information and contents on -but not limited to- chats, discussion boards, blog generators, comments, social networks, or any other means that allow third parties to freely publish contents on the provider’s website. Nevertheless, and to abide by the art.11 and 16 of the LSSI-CE, the provider remains accessible to all users, authorities and security forces, to actively collaborate on the removal or blocking of any content that could contravene the national and international legislation, the rights of third parties or the morality and public order. Whenever the user considers that a content on the website is infringing such conditions, he is prompted to warn the website administrator immediately.
This website has been revised and examined so that it works properly. It should be possible to guarantee the functioning of the website 365 days per year and 24 hours per day. However, the provider does not fully guarantee the absence of programming errors, nor the absence of other force majeure causes, natural catastrophes, strikes, or similar circumstances that would impede the access the website.
You use this website at your own risk. The Company, its partners, collaborators, employees or representatives do not assume liability for any errors or omissions in the content of this website or any other content that may be accessed through it. The Company, its partners, collaborators, employees or representatives also do not assume liability for any damage arising from use of this website, or for any other action performed on the basis of information available on it.
The Company does not guarantee absence of virus or other elements that could damage or alter your computer system, electronic documents or files. Therefore, the Company does not accept liability for any damage that these elements could cause you or third parties.
3. INTELLECTUAL AND INDUSTRIAL PROPERTY
The website, including but not limited to: its programming, edition, compilation, and other elements needed for its functioning, design, logotypes, text and images, are property of the provider or else the provider has got licence or specific authorisation of the authors. All the contents of the website are properly protected by the intellectual and industrial property regulations and are as well registered in the corresponding public files.
Users are not authorised, regardless of their purpose, to copy or display total or partially, distribute, use, exploit, commercialize the website, without the express written consent of the legitimate owner.
Improper or unauthorised use of the materials and information contained on the website constitutes an infringement of the intellectual or industrial property rights of the owner.
Designs, logotypes, text and/or images that may appear on the website, which are not property of the provider, are property of their owners, hence the owners are responsible of any possible conflict derived from them. In any case the provider has got the owners’ specific and prior authorisation of use.
(optional) The provider expressly authorises/does not authorise third parties to redirect to particular contents of the website. The link should redirect to the main website of the provider.
The provider accepts in favour of its owners the corresponding rights of industrial and intellectual property. The sole appearance or reference on the website does not imply the existence of rights or responsibility of the provider, or any sponsorship, support or recommendation by the provider.
Any observations on possible transgression of the rights of intellectual and industrial property, as well as on any of the contents of the website, can be sent to this email address.
4. LAW AND JURISDICTION
All and any matters which may derive from access to and/or utilisation of the website will be governed and construed in accordance with Spanish legislation, to be obeyed by all the parties involved.
Any conflict related or derived from the use of the website will be submitted to the Courts and Tribunals of Barcelona.
5.1. Company identification
We inform you that the provided data, will be processed by TRAFFIC NETWORK SOLUTIONS, S.L. (The Company) with registered office in Barcelona (08009), Calle Bruc pral 1b – Spain and VAT number B-64902430 with the purposes described in this chapter.
For data processing the Company operates in accordance with, and are fully committed to, the regulation in EU law on data protection and privacy for all individuals referred to as the General Data Protection Regulation (GDPR) (EU) 2016/679. The GDPR aims primarily to give control to citizens and residents over their personal data and to simplify the regulatory environment for international business by unifying the regulation within the EU.
You can contact with the Company through the following email address: hello [at] trafficnow.eu and phone number +34 93 205 85 62
5.2. Processing purposes
To attend user’s information requests and general enquiries through the Contact Us, Become a Partner and Investor Request form.
To send commercial information about our services or products.
To attend user’s job appliances through our “Apply now” form.
To help you with any trouble through our support platform.
5.3. Storage period of data
The provided personal data will be stored, unless the subject requests its erasure, according to the following terms:
Data related to a general request will be stored until the satisfaction of a user’s enquiry is reached.
Contact information and user preferences for sending commercial information via newsletters will be stored for two (2) years or until the user requests to have it erased.
Personal data related to job appliances will be stored for one (1) year.
Data concerning to support enquiries will remain stored for five (5) years from its resolution.
5.4. Lawfulness of processing
The legal basis for the data processing is user’s consent to it. This consent is requested through the company’s forms on the website.
Users do not have any obligation to provide us with their personal data. Notwithstanding, in certain cases, the absence of certain data could prevent us to attend user’s request properly or even at all.
5.5 Data receipts
The company may recruit third parties in order to offer its services.
No data will be provided to third parties. If any third party need data, you will be previously informed of the communication purposes and the identity of the third party and you will be asked to consent.
All of the above without prejudice of any legal requirement that demand us to communicate data to any authority or third party.
The individuals who provide us their data enjoy the following rights:
a. Right of access: The data subjects shall have the right to obtain from the company confirmation that their data are processed properly and they shall have the right of access their personal data.
b. Right to rectification: The data subject shall have the right to obtain from the company without undue delay the rectification of inaccurate personal data concerning his or her.
c. Right to erasure: The data subject shall have the right to obtain from the company the erasure of personal data concerning him or her.
d. Right to restriction of processing: The data subject shall have the right to obtain from the company restriction of processing where one of the following applies:
1. the accuracy of the personal data is contested by the data subject, for a period enabling the company to verify the accuracy of the personal data;
2. the processing is unlawful and the data subject opposes the elimination of the personal data and requests the restriction of their use instead;
3. the company no longer needs the personal data for the purposes of the processing, but they are required by the data subject for the establishment, exercise or defence of legal claims;
e. Right to data portability: The data subject shall have the right to receive the personal data concerning him or her, which he or she has provided to an organization, in a structured, commonly used and machine-readable format and have the right to transmit those data to another organization without hindrance from the company to which the personal data have been provided. Please have in mind that this right will not be enforceable to:
– The third parties’ data that you have provided to the Company.
– The data that concern you, but have been provided to the Company by third parties.
f. Right to object: The data subject shall have the right to object at any time to processing of personal data concerning him or her. You shall be able to object to the commercial communications submitting.
You can exercise this right by sending us an e-mail to the following address: hello [at] trafficnow.eu or by sending us a letter to Company’s address hereto. To exercise any right, you shall provide us with a copy of your ID card (DNI) and clearly express the right you want to exercise.
See the Spanish Data Protection Agency website for more details about your rights.
Additionally, we inform you about the possibility make a claim before the competent control authority, in this case, the Agencia Española de Protección de Datos, especially in case the exercise of your rights was not satisfactory. You can contact the control authority via their telephone numbers 901 100 099 y 912 663 517 or visiting their office at C/ Jorge Juan, 6. 28001 – Madrid.


text:
- Predict the long-term consequences of exponential human population growth
Human Population Growth
Global human population growth is around 75 million annually, or 1.1% per year. The global population has grown from 1 billion in 1800 to 7 billion in 2012. It is expected to keep growing, though predictions differ as to when and if this growth will plateau.
The “population growth rate” is the rate at which the number of individuals in a population increases in a given time period as a fraction of the initial population. Specifically, population growth rate refers to the change in population over a time period, often expressed as a percentage of the number of individuals in the population at the beginning of that period. This can be written as the formula:
population growth rate=P(t2)−P(t1)P(t1)population growth rate=P(t2)−P(t1)P(t1)
Globally, the growth rate of the human population has been declining since 1962 and 1963, when it was 2.20% per annum. In 2009, the estimated annual growth rate was 1.1%. The CIA World Factbook gives the world annual birthrate, mortality rate, and growth rate as 1.89%, 0.79%, and 1.096% respectively. The last 100 years have seen a rapid increase in population due to medical advances and massive increase in agricultural productivity.
Each region of the globe has seen reductions in growth rate in recent decades, though growth rates remain above 2% in some countries of the Middle East and Sub-Saharan Africa, and also in South Asia, Southeast Asia, and Latin America. This does not mean that the population is declining;
rather, it means the population is growing more slowly. However, some countries do experience negative population growth, mainly due to low fertility rates, high death rates and emigration.
According to the UN’s 2010 revision to its population projections, world population will peak at 10.1 billion in 2100 compared to 7 billion in 2011. However, some experts dispute the UN’s forecast and have argued that birthrates will fall below replacement rates (the number of births needed to maintain a stable population) in the 2020s. According to these forecasters, population growth will be only sustained until the 2040s by rising longevity, but will peak below 9 billion by 2050, followed by a long decline.
Long-term Consequences of Population Growth
The “population explosion” seen in the last century has led to dire predictions. In 1968, biologist Paul Ehrlich wrote, “The battle to feed all of humanity is over. In the 1970s, hundreds of millions of people will starve to death in spite of any crash programs embarked upon now. At this late date, nothing can prevent a substantial increase in the world death rate. ” Although many critics view Ehrlich’s view as an exaggeration, the human population continues to grow exponentially. The laws of nature dictate that exponential growth cannot continue indefinitely.
Despite efforts to curb population growth, such as the “one-child policy” in China (introduced in 1979 but relaxed in the early 2000s), the human population continues to grow. A primary concern regarding this growth is that the demand for ever-more food will lead to widespread shortages, as forecast by Ehrlich.
In addition to the threat of food shortages, human population growth is damaging to the environment in potentially permanent ways. Most scientists agree that climate change caused by the emission of the greenhouse gas carbon dioxide (CO2) is a significant consequence of human activities. In a series of treaties in the late 20th century, many countries committed to reducing their CO2emissions to prevent continuous global warming; however these treaties have not been ratified by every country, largely due to economic and political concerns. The role of human activity in climate change is hotly debated in some circles. The future holds considerable uncertainty for curbing human population growth and protecting the environment.
- Global human population growth is around 75 million annually, or 1.1% per year. The global population has grown from 1 billion in 1800 to 7 billion in 2012.
- Although the direst consequences of human population growth have not yet been realized, exponential growth cannot continue indefinitely.
- In the late 1970s, China’s “one-child” policy tried to control population growth, but restrictions were relaxed in the early 2000s.
- One of the major consequences of population growth is the potential for widespread food shortages.
- Most scientists agree that humans and human population growth are causing climate change by emission of the greenhouse gas carbon dioxide (CO2).
- International treaties to limit greenhouse gas emissions have not been ratified by every country due to economic and political concerns.
- greenhouse gas: Any gas, such as carbon dioxide, that contributes to the greenhouse effect (continued warming) when released into the atmosphere.
- climate change: Changes in the earth’s climate, especially those said to be produced by global warming.

domain: economics


text:
At Sunfox we screen our pet buyers to make sure there is a good match of kitten to new owner. When you contact us, be prepared to answer questions about your housing, family members, and past and current pet ownership, and please fill out our Inquiry Form. We require a veterinarian's reference. If you are new to pet ownership, now is a good time to start your search for a veterinarian you feel comfortable with. Also feel free to ask any questions you may have, we will do our best to answer.
If price is the most important issue for you, we suggest you look elsewhere - you can surely find a pet for less money if that is your main goal - our goal is to raise healthy, well-adjusted Somali kittens, and it's an expensive undertaking. Alternatively, just because you are willing to pay our asking price doesn't necessarily mean you will get a kitten from us - it's important that both the new owner and the kitten be happy, and that the kitten get proper health care and love and affection during its lifetime. Our pet buyers make a lifetime commitment to the health and happiness of the kitten.
All pet kittens are spayed or neutered before delivery to the new owner. This is an advantage to you as the future owner, as I take the risk of any problems, and is good for the kitten, as he or she recovers at home in familiar surroundings. Kittens are sold at 16 weeks of age at the earliest. At this age they have had all their kitten vaccinations and have been fully socialized. Sometimes we may keep kittens longer - Somalis are notoriously slow to mature, and sometimes it takes a few extra months to determine whether a kitten will be suitable for showing.
Sometimes buyers want to get their kitten at a younger age, feeling that a younger kitten will "bond" better with the new owner. This theory may apply to puppies, but does not apply to kittens. Kittens need to be properly trained and socialized by the adult cats and humans before they are ready to go to new homes. And since we give their final kitten vaccinations at 16 weeks, the new owner doesn't have to worry about vaccinations again until the cat is a year old. For more information, see the article entitled "How Young is Too Young."
Pet buyers sign a contract agreeing not to allow the kitten to roam outside, and not to declaw the kitten. The contract spells out the duties of both buyer and seller so that there are no questions about what was agreed to.
Sunfox kittens are guaranteed negative for FELV and FIV and free of fleas and fungus. They are fully vaccinated, and require no further vaccination until they are 16 months old.
We do not ship our cats. We live in Ventura County, California. If you live in a surrounding area, such as Northern California or Arizona, it is possible we can make arrangements meet you half way to deliver a kitten. Things may work out for someone to carry the kitten on board a flight for you, at a cost roughly close to the shipping cost. Keep in mind, however, that you would not be able to see the kitten before you get it, and this may not be the best idea - the best thing is of course, to come out here and meet the cat or kitten yourself. If none of these options are workable, we suggest you look for a breeder who is closer to you.
Thinking about an adult cat? Check out this video of Amelia, red Somali spay. Amelia was born in 2002, and this video was taken at Christmas 2008 when she was 6 years old. She was placed in the home at 4 with her daughter, Kismet, who was 3. Take a peek at this YouTube video of Amelia playing with her new bird toy.
To understand why breeders and exhibitors sometimes place adult cats, see this article.
We currently have several adult cats available, all female.
For rescued Abys and Somalis in Southern California, go to SCAR. They do have a couple of Somali rescues available, and several Abys.
We do have a black DSH spay called Ella Bella. I found her under my car at Target in October 2009, just before Halloween. She's best buds with My older boy Frankie, is probably 6 years old by now. Email us for more informaton.
Abyssinian and Somali - click here to find out the differences and why we work with both.
We feed and recommend Life's Abundance pet foods. Please see our website, www.HealthyCatndog.com. We do require new owners to purchase the food initially, to provide continuity to the cat's diet so as not to cause any tummy upsets during the very stressful rehoming process. In addition, we feel it is the best cat food on the market, and is more economical than almost all other premium brands, so we don't have any reservations in recommending it for the life of your new kitten!
Abyssinian kittens available! A breeder we work with in Denmark has 2 kittens available. Because there are many breeders in Denmark, which is such a small country, sometimes it is hard to find homes for them close to home. Here in the U.S., we have the opposite problem - not enough breeders for the people who want kittens. So I've agreed to help find forever homes for these kittens. Pricing will be the same price other breeders have here in So. California, plus shipping. Because shipping is expensive, the breeder is willing to work out a discount on the shipping, provided both kittens can travel together. That means either one family who wants both kittens, or 2 families from the same area (i.e., So. California) each want one kitten.
The kittens are ruddy boys. The older was born November 10, the younger born on December 1. They are sweet, happy, playful kittens. Traveling together will take a lot of the stress of traveling away.
Photos will be posted soon. Because the kittens will need to be neutered and have all their shots at least 21 days before traveling, they will need to be reserved no later than March 13.
If you might be interested, please email me asap at firstname.lastname@example.org. It would be useful if you would fill out the form at www.sunfoxsomalis.com/inquiry.html and mark that you are interested in the "Danish Abys" and I can put you in contact with the breeder.
I had my first breeding cat from this breeder, I have visited her home recently and highly recommend her cattery.
This is Baby, born December 2011. Baby had a wonderful home in Nevada, but sadly she was only there 2 years, and her human passed away. So Baby came home until we can find her the perfect home.
Misty is her mother, they are both blue.
Baby had a first home as a kitten, and was ambushed in the litter box by the other cats. So she needs some special litter boxes. She would not do well with other cats.
Finally, our 3d blue girl is Lil Bit, who is 11. Lil Bit lived in a home for 9 years and got along fine with the other cats. But then 2 big Maine Coon kittens came to live in the family, and they picked on Lil Bit, and even attacked her. So she's pretty timid now and probably needs to be the only cat in her home. She is very sweet and loving, she loves to cuddle in bed at night. She's really playful too, she'll chase feather toys if you will play with her. Soon we'll get a nice photo of Lil Bit up here too.

domain: pet


text:
Workers in the tourism industry have been told by Minister of Labour and Social Partnership Relations, Colin Jordan, that there is no room for discrimination in that sector.
The minister, a former tourism executive, emphasized this on Tuesday, while addressing a seminar on Inclusive Tourism in the Caribbean, hosted jointly by Virgin Holidays, B-GLAD, the Royal Commonwealth Society and the Canadian High Commission, at the Savannah Hotel.
Pointing out that the mindset of all, including those in the tourism industry, had to be that “we are human beings who are interacting with other human beings”, he stressed the need to treat others as we would want them to treat us.
Mr. Jordan said: “In tourism, there is no room for stereotyping…. Human beings fear the unfamiliar, and we sometimes have to train ourselves to be kind, understanding, and respectful. In tourism, there is no room for discrimination. We, as workers in this industry, do not discriminate because it is not the decent, human thing to do. We do not discriminate because our guests are human beings. When we check in a guest, we are concerned only with the ability of the guest to pay. We do not ask if the guest has a temper, and is prone to destroy hotel furnishings. We accept people as they present, and we work to make their stay an enjoyable one.
“Discrimination is an issue that must be addressed because it speaks of treating to some…as inferior and as not deserving of the same level of treatment and respect as those of the main group(s). There are those who are traditionally discriminated against because of their ethnicity; racial identity; gender; sexual orientation; a disability; gender identity; religion; or in some cases their denomination.”
The Labour Minister further reminded those gathered that his ministry was currently preparing legislation to address the issue of discrimination in the workplace, and put a framework in place to prevent its occurrence.
Elaborating on the importance of this, he said: “Secondary to being the right thing to do, there are genuine economic benefits to rejecting stereotypes and discrimination, and rather being inclusive in our approach to the tourism industry.”
Tourism, he added, was the main driver of the Barbados economy, earning approximately half of the country’s foreign exchange, and employing more people than any other single industry.
“Significantly, for a country like Barbados, tourism employs people across a wide range of skill sets, abilities, and levels of certification. Tourism employs many people with degrees and who have no certification. Tourism is, therefore, absolutely essential in maintaining our social and economic stability,” he said.
The minister also stated that along with its related sectors, tourism was responsible for much of the country’s Gross Domestic Product (GDP) and much of the GDP of the Caribbean as a whole, which is the world’s most tourism-dependent region.

domain: sociology


text:
Ne-Yo has done it again. The multi-platinum artist and songwriter is back with his latest single, Start A Fire Music, and it’s sure to ignite the hearts of music lovers everywhere. With an infectious beat that will have you tapping your feet, this new track from Ne-Yo offers a fresh perspective on life through its powerful lyrics. But what makes Start A Fire Music so special? Let’s take a closer look at how Ne-Yo has used his talents to create yet another masterpiece.
The first thing one notices about Start A Fire Music is its captivating melody. From the moment the song begins, listeners are pulled in by the catchy chorus and soothing vocals that come together to make for an incredibly memorable listening experience. Not only does this track feature some amazing production work but also smartly crafted lyricism as well. Ne-Yo delves deep within himself to touch upon themes such as love, self-discovery and personal growth; all of which can be felt as soon as one starts singing along with him.
Finally, there is something inspiring about Start A Fire Music that really sets it apart from other songs out there today: hope. Throughout the entire track, Ne-Yo encourages us to never give up even when times get tough and reminds us that our dreams can still become reality if we just believe in ourselves enough to keep pushing forward. This uplifting message is truly what brings this already incredible piece of artistry full circle and gives fans around the world something they can relate to on a much deeper level than ever before!
Biography Of Ne-Yo
Ne-Yo is a multi-platinum American singer, songwriter, record producer and actor. He has earned numerous awards throughout his career, including three Grammy Awards and two BET Awards. Ne-Yo first rose to fame in 2004 with the release of his debut single “Stay” from his album In My Own Words. Since then, he’s released seven studio albums, selling over 25 million records worldwide.
Ne-Yo’s music style incorporates elements of R&B, soul and hip hop. His lyrics range from inspirational messages about self-empowerment to more romantic topics such as love and relationships. He often collaborates with other artists on his tracks, lending his vocal talents to create memorable hooks and choruses that captivate listeners around the world.
In 2020, Ne-Yo released Start A Fire Music – an EP featuring five new songs written by himself along with various producers. The project showcases Ne-Yo’s lyrical prowess as well as creative use of samples ranging from classic funk to modern day rap beats. The title track serves as an ode to hope and resilience during challenging times while also providing inspiration for listeners looking to start their own fires in life.
Analysis Of ‘Start A Fire’
As one of Ne-Yo’s most recent releases, Start A Fire Music is an uplifting and inspiring track filled with thoughtful lyrics that speak to the heart. The song begins with a slow but powerful beat as Ne-Yo croons about how “It’s never too late to start anew / To rise up from our blues and make something beautiful out of life.” This thought-provoking lyric serves as an invitation for listeners to use their own stories and experiences to create something meaningful in their lives.
The chorus of this song then takes on an upbeat tone – both musically and lyrically – as Ne-Yo encourages people to “Start your fire, light it higher/ Believe in what you can do.” Here, we see his belief in each individual’s ability to overcome adversity and achieve their goals despite all odds.
Ne-Yo has managed to capture the essence of indomitable spirit through this single – providing hope, inspiration, and motivation for those who are ready to take control of their destiny by starting a fire within themselves. With its positive message, unique production style, and captivating vocals, Start A Fire Music stands out among other R&B songs released in 2020.
I. Summary of Ne-Yo’s Career
In conclusion, I have discussed the career and music of Ne-Yo, an artist who has released seven studio albums since his debut in 2006. His success is due to his soulful voice, catchy hooks and smooth melodies that appeal to a wide range of audiences. He has also collaborated with many big names in the industry, such as Rihanna, Pitbull and TLC.
II. Analysis of ‘Start A Fire’
His latest single ‘Start A Fire’ is a testament to his skill as an artist – it speaks directly to his target audience and encourages them to take action and break free from their current circumstances. The song features a strong beat along with inspirational lyrics that make for an uplifting tune overall.
III. Final Thoughts
All in all, Ne-Yo is one of the most successful artists today because he knows how to capture people’s attention through thought-provoking lyrics and unique musical styles. With ‘Start A Fire’ he continues to prove why he deserves a spot amongst music royalty – this track will certainly be remembered long after its release!
Since 2005, Singersroom has been the voice of R&B around the world. Connect with us via social media below.

domain: artistic


text:
What continually fascinates me about rural Hunan is the selection of vegetables – and the amount of chilli – one finds so easily.
Predominantly, coming from small patches between the rice fields.
Rice is the dominant agricultural product of southern China, and especially of Hunan. The many lower-lying grounds, which the surrounding hills and mountains supply with copious amounts of water, are excellent for growing rice. The subtropical climate also does its part.
Some other things like “vineyards” also get more room, because the grapes bring good money.
Much of the vegetable growing, though, is done on small patches. It can be found on soil between buildings and roads. Sometimes it happens on minuscule amounts of soil in interior courtyards of apartment buildings that are otherwise pure concrete.
Sometimes, the vegetable patches are between rice fields, like the one that I had a closer look at.
Dry Ground in Wet Rice
First thing to notice: This vegetable patch was in the midst of the wet rice cultivation, in fields which are flooded – but many of the plants here looked rather dry.
Water spinach was growing there, so it couldn’t have been too dry, at least for plants that root deeply enough. The chilli certainly showed, once again, that it is not the most deep-rooting of plants.
Off and on, in other places and especially for chilli, I have seen farmers separately water the chilli from the irrigation canals for the rice fields. It doesn’t happen too often, though.
The way that different species and varieties of plants are organized is quite interesting. Everything has its place, to some extent, but everything is also mixed together somewhat. And sometimes, even more.
Here, for example, there was a definite patch of sweet potatoes at one end. Then came bitter melon, which already seemed mixed with another vine-like plant, perhaps pumpkin.
Some amaranth was growing close to the ground, less recognizable as a vegetable of its own (and not exactly on its own). Then came a definite pumpkin and, probably, some kind of bean, on trellises to climb up.
Aubergine (eggplant) could be found; soy beans, more aubergine and then finally chilli.
More chilli was growing a bit farther away; that one was more obviously of the longer type that is most commonly used in Hunan. A step further, somewhat separate, grew chaotianjiao, the “facing heaven chilli” that is rather more pungent.
The Thing about Chilli Varieties
In the mixture of pod shapes to be found among that chilli, it is quite obvious that these were landraces. At least, the farmer used their own chilli seeds rather than uniform seeds true-to-type, bought from some breeder.
That brings us to the typical problem: There is some diversity, not an obvious variety of chilli. It is just what the farmers grow, what may have been grown for longer already. Or not that long.
Ask someone, what they are growing, and the answer is likely to be the usual: Green chilli or red chilli, long chilli or chaotianjiao. But between different places, these same names will likely mean a type of chilli that is at least somewhat different.
The differences only become relevant, when they become relevant. Either because someone like me goes there and collects some chilli, and has a need to separate those from one place versus another.
Or because the market develops in such a way that farmers no longer sell only on the local market, what they usually sell there (like everybody else), but because there is some interest in the local-authentic of a place. Then it becomes necessary to market things better, to make them differentiated from the others’ products!
Vegetable Diversity on Patch and Plate
It is also always interesting, what one finds grown versus known from dishes – or less known.
Kongxincai, the water spinach, for example, is a very popular vegetable.
Soy beans, when they are grown in such small amounts, are sure to be used as maodou. Fresh, as those “hairy beans”, they are often boiled in their pods. In Hunan, this preparation that is otherwise the same as the Japanese edamame ends up rather heavily aromatized, though.
Or they are shelled, and stir-fried with green chilli and garlic.
But then, there was also okra in this field. Sometime I seem to remember stumbling across its use in China. It was only this summer that I actually ate okra in China for the first time, though – and then promptly found it growing in this vegetable patch, as well.
I had never seen it in China before.
Talking of things (not) seen: The mysterious vegetable I mention at the end of the video is sure to have been malabar spinach.
What always misleads me about it is that the one I grow myself climbs strongly as a vine, while in China, I always see it grown close to the ground.
In food, I have regularly seen it, but only used in one kind of soup that I find to have a rather odd taste…

domain: biology


text:
- Kanye West is not headed to an American Idol reboot - TMZ
- Liam Payne had a sweet message for his fans after the birth of his son - Gossip Cop
- Paris Jackson is growing out her armpit hair - Just Jared Jr
- Nick Viall and Vanessa Grimaldi had another hometown date! - Wetpaint
- Benedict Cumberbatch just welcomed his second son! - Lainey Gossip
- Cash Cab is getting a reboot! - The Hollywood Reporter
Bar Refaeli Covers 'Grazia' Magazine's Latest Issue!
About Photo #2951381: Bar Refaeli grabs an iced coffee to go with some male pals on Thursday (September 12) in Malibu, Calif. The 28-year-old model recently took the cover of Italy's… Read More Here

domain: celebrity


text:
LEWISTON – The jury trial for Clyde Ewing, Demetri Ewing’s dad will begin Friday, May 13 with jury selections with Nez Perce County Judge Jay Gaskill presiding over the case.
Ewing is being tried for first-degree murder for his alleged role in the shooting, and ultimately death of Samuel Johns, on January 8, 2021. Demetri was tried as an adult for first-degree murder regarding the same incident and was found guilty on Thursday, April 21.
Clyde will be represented by defense attorneys Rick Cuddihy, and Sean Walsh. The state will be represented by Elected Prosecutor Justin Coleman and Chief Deputy Prosecutor April Smith.
“My office shifted our focus [to] Clyde Ewing’s trial immediately upon the conclusion of the co-defendant, Demetri Ewing case in April,” Prosecutor Justin Coleman said. “We are looking forward to bringing Mr. Ewing to justice for his role in this murder.”
If found guilty, Ewing could be sentenced to life in prison without the opportunity for parole.


text:
Architecture and design media practice SGMStudio has filmed a short documentary visualising four seasons of the Sancaklar Mosque from frame to finish
The film visualises the transformation of steel, concrete and stone into a place of worship by following the construction of the building from frame to finish every step of the way. It premiered at Architecture Week Prague.
The Emre Arolat Architects-designed mosque - located on the outskirts of Istanbul - is a distinguished example of modern architecture that stands out among Turkey's Islamic places of worship dominated by historicist building typologies.
In order to reflect and project the creation process of this unique piece of architecture, Istanbul-based SGMStudio documented Sancaklar Mosque from the ground up for more than two years.
The creative team narrates the building's architectural emphasis on "the tension between the natural and the artificial" via motion picture -a fairly unusual medium among the usual representations of architecture- and adopts the seasonal changes witnessed by the construction process as the back bone of the story.
‘Sancaklar Mosque' depicts the transformation of the building from a framework to a place of worship, conveys the integration of the fascinating natural landscape of the project with the fluid, man-made landscaping, and introduces the building's proud and graceful geometry through the agency of motion picture and documentation.
The film visualises the seasonal changes in activity and the organic transformation of the landscape, not as mere backdrops, but as aspects of the mosque's formation. SGMStudio aims to portray the building's extraordinary and striking spatial sensation by enhancing the same sensibility visible in the structure within its narrative.
For ‘Sancaklar Mosque', SGMStudio spent 35 shooting days spread over a period of 24 months. Due to the inaccessibility of the area during harsh winter times, the studio recreated the snowbound imagery of the construction with the help of photo manipulation, matte painting, 3D effects and compositing techniques.
By utilising both sharp timelapse shots and cinematic footage for its visual narrative, the film dwells on the tension between the technical and engineering-centric implementation and humble and tranquil art of building with its pacing.
The original score of the film is an integrated manifestation of both the modern silhouette and the Islamic references of the building. The musical piece combines classical music with the oriental end-blown ney flute, allowing the co-existence of the hectic beat of construction and the peaceful serenity of worship within one picture.
For the musical composition, which steers the pace and feel of the film with the help of audio samples of natural phenomena, all of the instruments, including the ney, were mixed in a fully digital environment.
Sarraf | Galeyan | Mekanik (a.k.a. SGMStudio) was founded by partners Emine Seda Kayim, Erhun Erdogan and Memduh Can Tanyeli in August 2012. Defining and creating an inter-disciplinary working field emerging from the individual specialty areas of its partners, SGMStudio has drawn its path by designing and producing multimedia works for creative industries,

domain: design


text:
Koch Media has announced that Superbike World Championship 07 will be released on the PS2, PSP, PC and now Xbox 360. This one is developed by Milestone, the same guys that did the first Superbike World Championship back in 1999. It did pretty good and got solid reviews, so this can turn into something good. While the Xbox 360 and PC versions will be released this autumn, the PSP and PS2 versions will be released in a couple of months. Inside you'll find some new images from the PS2 version of the game.
I explained to her that sitting on my front porch on a backstreet that no one uses is only going to get my attention. (19 minutes ago)
Speaking of mental illness: woke up this morning to some crazy woman "protesting" on my front porch. I'm still not sure what she was protesting. Something about land management. (20 minutes ago)

domain: entertainment


text:
Revolution isn't just a vodka bar. They serve up plates nachos to share, but if you and your mate aren't really in the mood for nachos, check out the "Revolution Platter". This is a combination of smoky chilli, tortilla chips, southern fried chicken goujons, homemade potato wedges, beer battered onion rings with BBQ sauce and their homemade vodka chilli salsa. That's sure to satisfy a hungry stomach.
Bars With Food Platters in Glasgow
Food platters are perfect for when you're grabbing a bite with a friend and can't decide what to have, or perhaps you want to feed the masses with some light bites at your birthday party. Thankfully, Glasgow's bars have got the whole sharing platter thing sorted. From casual pubs serving up good old pub favourites and nachos to swish bars specialising in buffets for larger groups, take a look at our picks of bars with food platters in Glasgow and be prepared next time you venture out with a hungry group of friends.
As you'd probably expect from this chic Glasgow bar, Hummingbird up the ante when it comes to sharing platters. Instead of your regular sharing combo, Hummingbird offer a pretty swish buffet with mini burgers, haggis balls, mini fish suppers and their so called "posh chips". If that's not enough, you can even upgrade to their £20 buffet which has a wider selection of food and half a bottle of wine per person. Hummingbird is the perfect choice for people looking for a fancy bar with food platters in Glasgow.
Stereo serves up a brilliant vegetarian sharing platter. Served every Monday, Stereo offer five plates from their tapas menu for £12. Take your pick from roasted vegetables, vegetable tempura, sweet potato chips, and falafel. Definitely one of the best sharing platters in Glasgow for vegetarians or people who like to eat fresh.

domain: food


text:
October 19, 2016 by Virginia
I’ve made Chia Pudding before, and loved it! So I figured, what could make my Chia pudding recipe better? Pumpkin was the answer and it made it so good!
Chia seeds are amazingly versatile- use them in smoothies, to top your salads, egg replacements in baking -even as a coating for baked tofu! The best thing about these little protein packs, is that they create this gel-like consistency when soaked. This mimics the consistency and texture of traditional pudding. The seeds become these soft gooey balls that basically taste like whatever you mix them in, and they do a great job of absorbing flavors.
So here’s my experimental chia pudding recipe that made it taste like Pumpkin Pie. Try it out and let me know what you think!
Pumpkin Pie Chia Pudding
- 1 1/2 Cup Almond Milk*
- 1 Cup Pumpkin Puree
- 1/4 Cup Chia Seeds*
- 2 Tablespoons Agave Nectar*
- 1 tsp. Vanilla Extract
- 1/2 tsp Pumpkin Pie Spice
- Combine all ingredients in a bowl. Mix for a full minute or so to make sure that everything gets mixed up and distributed evenly.
- Refrigerate for at least 40 minutes (the longer in the fridge the better!)
- Serve in glasses and top with pumpkin seeds, Pecans or coconut whipped cream.
Makes about 3 servings depending on how you like to divide it up!
That’s it! it’s literally as easy as 1-2-3!
- *Almond Milk: I’ve found that almond milk or coconut milk work best, but for this specific recipe, Almond milk compliments the pumpkin flavors best.
- *Chia Seeds: I used black chia seeds, you may use any color chia seeds so long as they are CHIA.
- *Agave Nectar: I used Agave Nectar Light, but you can use regular -or maple syrup as a substitute.


text:
Publish: 27 Jun 2020, 08:44 pm
The Bangladeshi embassy in Beijing approached the North Korean embassy in China many times but there has been no response
The Democratic People's Republic of Korea still owes Sonali Bank at least $11.62 million for goods imported under a barter agreement 26 years ago.
Under the Barter 5 agreement, North Korea bought goods worth $6.14 million from Bangladesh, but it did not pay for the purchase. The absolute arrears of Barter 5 were then transferred to Barter 6, which was signed on September 12, 1994, standing at $6.26 million.
On March 31, 1995, the Barter 6 agreement expired without any transactions.
Since then, the Bangladeshi embassy in Beijing has contacted the North Korean embassy in China many times to ask them to pay the payments, but no response has been received.
The state-owned Sonali Bank has now sought the help of the Financial Institutions Division (FID) to take effective steps to protect the bill through the Ministry of Commerce.
When asked, Sonali Bank Managing Director and CEO Ataur Rahman Prodhan told The Business Standard on Saturday, "It is a very old incident and I do not properly recall the information about this at the moment."
However, other reports from Sonali Bank and the Ministry of Commerce announced that the first Barter Agreement between Bangladesh and North Korea had been concluded on 12 August 1977.
Within the deal, Sonali Bank was appointed on behalf of Bangladesh and the Foreign Trade Bank was designated on behalf of North Korea for banking operations.
Both banks also signed an agreement to perform banking functions on behalf of their respective nations after the signing of Barter 6 in 1994. This interbank agreement included a provision of charging interest at the three months USD LIBOR rate.
In a letter to the FID, Ataur Rahman Prodhan wrote that, after numerous pleadings, the North Korean Foreign Trade Bank reported Sonali Bank 's debt of $10.04 million on 30 June 2012 via a SWIFT post.
Although the North Korean bank has been repeatedly been asked to pay the dues, they are not paying at all, he added in the letter.
By 2014, the debt had increased to $11.62 million. That year, the Ministry of Commerce sent a letter to the commercial counselor of the Bangladeshi embassy in China to take proper initiatives so that Sonali Bank could get its dues.
After that, the Bangladeshi embassy wrote a letter to the North Korean embassy in China demanding payment of the bill in vain.
Sonali Bank was not in a position to compensate for the full balance of the debt owed by the Democratic People's Republic of Korea.
Under the barter agreements, North Korea imported: rice, cement, jute and jute goods, urea fertilizer, tea, animal hide and skin, leather and leather goods, soap, detergent, toiletries, plus glycerin from Bangladesh.

domain: politics


text:
C10. Macromolecule Oligomer Formation and Symmetry
Many proteins are found in aggregated states and have quaternary structure. Hemoglobin consists of two alpha and two beta monomers (or protomers) which assemble to produce the biologically relevant heterotetrameric protein. As we discussed in Chapter 5A - Reversible Binding I, a given monomer can self aggregate to form homooligomers (dimers, trimers, tetramers, or Mn). The polymers display symmetry with respect to the geometric arrangement of the subunits. Symmetry, as we have just seen, is an important component of the MWC model. Most oligomeric proteins contain protomers that are symmetrically arranged. What mechanism determines whether a monomeric protein forms a homooligomer? Why do they stop at a certain n value? Can proteins be engineered to do so? If mutation can induce oligomer formation, then fewer mutations would be required to produce a symmetric oligomer from subunits since fewer mutations would be required as a single mutation in a single monomer would be represented n times in a single oligomer of n monomers. This fact probably underlies the reason that oligomers display exquisite symmetry. Hence a basic knowledge of symmetry of protein oligomers is necessary.
In the study of small molecules, chemists describe symmetry through the use of mathematical symmetry operations and elements, which find great use in analysis of structure and in molecular spectroscopy. These concepts are usually first encountered in physical and inorganic chemistry classes. A symmetry operation is a movement of an object like a molecule that leads to an identical, superimposable molecule.. Each operation has a symmetry element (point, line, or plane) about which the motion occurs. Some examples are shown below:
Table: Symmetry Elements and Operations
|Element (with Jmol link)||Operation|
|inversion center (i)||projection through center (point) of symmetry of point x,y,z to point -x,-y,-z|
|proper rotation axis (Cn)||rotation around a Cn axis by 360o/n where C denotes Cyclic|
|horizontal (σh) and vertical (σv) symmetry plane||reflection across a horizontal or vertical plane|
|improper rotation axis (Sn)||rotation around a Sn axis by 360o/n followed by reflection in plane perpendicular to the axis.|
Luckily for students trying to apply these rules to protein oligomers, biomolecules made up of chiral monomers (such as the L-amino acids of proteins) can not be converted to identical structures using inversion or reflection since the chirality of monomer would change - for proteins this would entail and L to D amino acid change. That excludes all but proper rotation axes (Cn) from the list above.
A point group is a collection of symmetry operations that define the symmetry about a point. The 4 types of symmetries around a point are those described above: rotational symmetry, inversion symmetry, mirror symmetry, and improper rotation. The types of point groups around a point include:
cyclic (Cn) - contain one single Cn rotation axis. A biological example is the tobacco mosaic virus double disk (34 monomers, C17). In this point group note that the n in Cn is equal to the number of monomers and the angle of rotation is 360o/n.
Figure: C2 Symmetry
dihedral (Dn) - These have mutually perpendicular rotation axes. Specifically they contain at least 1 C2 axis perpendicular to a Cn axis (Canter and Schimmel. Biophysical Chemistry - Part 1). The minimal number of subunits is n. Most protein oligomers fall into this category. The packing (or asymmetric) unit does not have to be a single monomer but could be a heteodimer.
A D2 point group has 1 C2 axis and 2 perpendicular C2 axes, and 4 monomers (like Hb). These proteins can dissociate into two dimers (such as two α/β dimers for Hb). Note that a different arrangment of 4 monomers could produce a oligomer with C4 symmetry instead of D2.
A D4 point group has 1 C4 axis and 4 C2 axes, along with 2n=8 subunits. An example of a D4 point group is ribulose bisphosphate carboxylase/oxygenase (RuBisCO) which has 8 subunits (where a subunit, or more technically the assymetric subunit, is a dimer of a small and large molecular weight protein). This point group could arise from quaternary structure of two C4 tetramers or four C2 dimers.
Figure: D2 Symmetry
cubic - contain four C3 axes connecting opposite corners of a cube (so the lines are effectively diagonals) arranged as the four body diagonals (lines connecting opposite corners) of a cube. The tetrahedron (4 sides), cube (6), octahedron (8), and icosohedron (20), perfect Platonic solids (in which all faces, edges and angles are congruent) all have related 3 C3 axes (diagonals connecting opposite corners for cubes, diagonals from a vertex to the opposite face for tetrahedrons, line connecting two opposite faces for octahedron, etc ) so they all can be considered to be part of the cubic point group.
Cubes have a total of 13 symmetry axes comprising 3 types (three C4 axes passing through the centers of opposite faces, four C3 axes passing through opposite vertices, and six C2 axes passing through the the centers of opposite edges). On octahedron can be aligned with a cube and be shown to have the same symmetry axes.
Flash movie: Cube (red) and Octrahedron (blue) with 13 symmetry axes.
Tetrahedrons have a total of 7 symmetry axes comprising two types (four C3 axes of the cube and three C2 axes which are the same as the C4 axes of the cube. First note the relationship between a cube and an inscribed tetrahedron.
Flash movie: Tetrahedron with 7 symmetry axes.
A dodecahedron with 12 regular pentagon faces (green) and an icosohedron with 20 equilateral triangle sides (red) can be aligned with each other (as can cubes and octahedrons) and have 31 symmetry axes, as shown below. Note also the relations between a cube inside a dodecahedron and a octahedron inside of a dodecahedron that makes sharing of symmetry axes between these pairs more obvious.
Flash movie: dodecahedron and icosohedron with 31 symmetry axes.
vrml files for movies from http://www.georgehart.com/virtual-po...etry_axes.html
Examples of protein complexes with these point groups are:
aspartate-ß-decarboxylase, tetrahedral, 12 asymmetric units
dihydrolipoyl transsuccinylase, octahedral, 24 asymmetric subunits
many spherical viruses, icosahedral, 60 asymmetric units
Jmol: Updated Symmetry in Protein Oligomers (beta version with lots of work left to do) Jmol14 (Java) | JSMol (HTML5)
Proteins, especially those involved in cytoskeletal filaments, can form fibers which contain helical symmetry which differs from those described above since the monomers at the ends of helical fibers, although they have the same tertiary structures as those in the middle of the helical fibers, do not contact the same number of monomers as monomers internal in the oligomer and hence have different microenvironments.
A recent article by Grueninger et al. addresses the question of whether the process of oligomerization can be programmed into the genome. Can simple amino acid substitutions lead to oligomerization? Remember that oligomerization can be beneficial (formation of cytoskeleton filaments) or detrimental (formation of fibers in sickle cell anemia and mad cow disease). Oligomers with long half-lives (for example cytoskeletal filament such as actin and tubulin) and short half-lives (for example proteins causes transient activities are regulated by oligomer formation) are both necessary.
It has long been noted that if a protein chain forms oligomers, then a single amino acid change in the chain would be found n times in an oligomer of n chains. Mutations could either promote chain contact and oligomer formation or dissociation into monomeric or other asymmetric subunit composition if the mutation were in a region involved in subunit association (a contact region). Experimental work in this field of study is hampered by the fact that mutants made by site-specific mutagenesis to prefer the monomeric state often fail to fold (due to hydrophobic exposure and aggregation. Studies have shown that most contact areas between monomers or other asymmetric units are hydrophobic in nature and the contact regions must be complementary in shape. Obviously mutations that replace hydrophobic side chains involved in subunit contact with polar, polar charged, or bulkier hydrophobic side chains would inhibit oligomer formation.
Grueninger et al were able to successfully engineer dimer formation and oligomer formation as well. First consider the simplest case of a mutation in a monomer that can produce a dimer with C2 symmetry. This is illustrated below which also shows how a mutation that produces a weak interaction in a monomer could also produce a long helical aggregate (which can't be crystallized) without symmetry (as described above). A mutation at 2 could promote either oligomer helix formation or dimerization.
Figure: Mutations causing Dimer with C2 symmetry or Infinite Helix
(adapted from Grueninger et al. Science, 319, 206-209 (2008)
It should be noted that mutation could lead to dimer or oligomer formation by producing a more global conformational change in the monomer (not indicated in the example above) which leads to aggregate formation, as we have seen previously in the formation of dimers and aggregates of proteins associated with neurodegenerative diseases (like mad cow disease).
Grueninger produces mutants of two different proteins that showed dimer formation as analyzed by gel filtration chromatography (but did not crystallize so no 3D structures were determined). In addition the group modified urocanase, a C2 dimer, at 3 side chains to form a tetramer with D2 symmetry. Also, they modified L-rhamnulose-1-phosphae, a C4 tetramer, at a single position to form an octamer with D4 symmetry. The latter two were analyzed through x-ray crystallography. Their work suggests ways that complex symmetric protein structures arose in nature from simple mutation and evolutionary selection.


Everybody makes mistakes — that’s a given — but we don’t always learn from them. Sometimes we make the same mistakes over and over again, fail to make any real progress, and can’t figure out why.
“Mistakes are always forgivable, if one has the courage to admit them.” — Bruce Lee
When we make mistakes, it can be hard to admit them because doing so feels like an attack on our self-worth. This tendency poses a huge problem because new research proves something that common sense has told us for a very long time: fully acknowledging and embracing errors is the only way to avoid repeating them.
Yet many of us still struggle with this.
To read the rest of the article, click here.


GoldLink has just shared a new song called ‘Justine’s Interlude’ that is all about his good friends, such as Justine Skye and the late Mac Miller. The song was debuted on A COLORS SHOW which increases its impact with the visuals. In the song GoldLink also touches on the alleged domestic abuse Justin had to endure in her past relationship. This new song is a great way for GoldLink to start off his new year which probably signals that he a new body of work on the way.
Join the Hype fam and sign-up today
Newsletters • competitions • events