text:
Review – hard punching Prize Fighter
The cast, from left: Thuse Lekwape, Pacharo Mzembe, Sophie Emberson-Bain, Kenneth Ransom, Margi Brown Ash and Gideon Mzembe.
After party photos by Deanne Scott follow the review.
By Future D. Fidel
Directed by Todd MacDonald
Dramaturg Chris Kohn
La Boit/Brisbane Festival production
Season: Sweptember 5-26. :Bookings: 07 3007 8600 or www.laboite.com
As we filed into La Boite’s Roundhouse Theatre the stage was set as the square ring – the boxing arena. Boxers sparred and trained, skipping rope, punching the heavy bag, doing push-ups while being urged on by a thumping sound track and the bellowing voice of trainer Luke, played beautifully by the diminutive Margi Brown Ash.
It was a clever recreation of a day in any amateur boxing gym helped along by great video design from optikal bloc, moody lighting from David Walters and music and sound by Felix Cross.
Among the boxers was Isa played by Pacharo Mzembe. He sparred for a good ten minutes before the story actually began and then he was exchanging realistic punches with his opponents as his tale unfolded. He had turned professional, was nicknamed Steven “the killer” Aliki and become a championship contender. We watched as he fought and his tale of oppression, violence, and hope unfolded.
For Mzembe the action is non-stop and his energy was unflagging. I knew he had been training up for the role – and his rippling shoulder and chest muscles paid tribute to this – but he was amazingly close to professional fitness.
I did a little boxing in my youth and I know how heavy the arms became after just a couple of minutes of defending and jabbing; and how the chest heaved and screamed for air. Mzembe did all that realistic and magnificently choreographed fighting (fight director was Nigel Poulton) for an hour – and managed to deliver his lines without a gasp.
What an amazing performance that was.
Future D. Fidel’s play is fictional but is inspired by his own life, the devastation of his family by the civil war in the Republic of Congo, his flight to Tanzania and his search for his lost sister before finally being accepted as a refugee in Australia.
It is a tale of his and many others fight for life recreated through the boxing ring where Isa’s tale is told between rounds as he fights for acceptance. We saw him in the Congo as a bewildered child when murder and rape surrounded him; as a refugee in neighbouring Tanzania, as a forced fighter being encouraged to rape and kill; being interrogated by the Australian immigration officials and learning to control his anger in the boxing ring.
Helping to tell Isa’s story is an ensemble of top talent with NIDA graduates, film and TV actors from very different backgrounds. From Zimbabwe there was Pacharo and his brother, Gideon who played several roles including, with his equally toned body, Isa’s final ring opponent in the championship fight, as well as a bullying army commander and sibling Moses.
Sophie Emberson-Bain, from the British Virgin Isles played three different roles each completely different and each perfectly played; South African-born Thuse Lekwape, a NIDA graduate making his theatrical debut shone too, particularly as the bloodthirsty soldier.
Kenneth Ransom began his career in the US in the TV series Fame and later played opposite Paul Hogan in Crocodile Dundee. After he moved to Australia he became busy with many state theatre companies and showed his versatility by playing four different roles convincingly.
The show ran for just over an hour and earned rapturous applause at the end.
It was an experimental show that worked for the most part. I, along with many others, did have some problems attuning to the authentic but strong African accents. I am sure the cast is capable of toning them down a little for clarity’s sake.
Meet the champs - Eric Scott with brothers Pacharo anmd Gideon Mzembe
Gideon Mzembe, Sophie Emberson-Bain and Margi Brown Ash
Director Todd MacDonald and writer Future D. Fidel.
Guests Helen Howard and Michael Futcher with Pacharo Mzembe
Listening intently to the speeches.

domain: entertainment


text:
Marc Staal sat out Saturday's game with an undisclosed injury but says that he will return to action for Game 1.
Rangers coach Alain Vigneault is claiming that Marc Staal isn't being rested on Thursday and instead has an undisclosed injury.
Marc Staal will not participate in Thursday's contest.
Marc Staal scored his fourth goal of the season on Saturday.
Marc Staal scored a goal in a 6-3 win against Florida.
The New York Rangers have finalized Marc Staal's contract extension on Sunday.
Larry Brooks of the New York Post is reporting that Marc Staal and the New York Rangers have agreed to a six-year contract extension.
Marc Staal and the New York Rangers are reportedly close to agreeing to a six-year contract extension.
Marc Staal played on Saturday after attending the funeral of his grandfather in Thunder Bay, Ontario Friday.
Marc Staal and Dan Boyle missed practice on Friday for personal reasons but both will be in the lineup Saturday.
Marc Staal scored for the first time this season on Saturday.
Marc Staal is reportedly seeking a six-year extension worth about $6 million annually.
Marc Staal and Rangers brass are still not close on inking an extension despite dialogue.
Marc Staal's agent, Paul Krepelka, told ESPN.com via email that it's "all quiet on that front right now," while referring to contract talks with the Rangers.

domain: news


text:
Acts Chapter 5
1 But a certain man named Ananiaswith Sapphira his wifesolda possession
2 And kept back part of the pricehis wife also being privy toitand brought a certain partand laid it at the apostlesfeet.
3 But Peter saidAnaniaswhy hath Satan filled thine heart tolie to the Holy Ghostand to keep back part of the price ofthe land
4 Whiles it remainedwas it not thine ownand after it wassoldwas it not in thine own powerwhy hast thou conceivedthis thing in thine heartthou hast not lied unto menbutunto God.
5 And Ananias hearing these words fell downand gave up theghostand great fear came on all them that heard thesethings.
6 And the young men arosewound him upand carried him outand buried him.
7 And it was about the space of three hours afterwhen hiswifenot knowing what was donecame in.
8 And Peter answered unto herTell me whether ye sold the landfor so muchAnd she saidYeafor so much.
9 Then Peter said unto herHow is it that ye have agreedtogether to tempt the Spirit of the Lordbeholdthe feet ofthem which have buried thy husband are at the doorand shallcarry thee out.
10 Then fell she down straightway at his feetand yielded up theghostand the young men came inand found her deadand carrying her forthburied her by her husband.
11 And great fear came upon all the churchand upon as many asheard these things.
12 And by the hands of the apostles were many signs and wonderswrought among the people and they were all with one accordin Solomon s porch.
13 And of the rest durst no man join himself to thembut thepeople magnified them.
14 And believers were the more added to the Lordmultitudes bothof men and women.
15 Insomuch that they brought forth the sick into the streetsand laid them on beds and couchesthat at the least theshadow of Peter passing by might overshadow some of them.
16 There came also a multitude out of the cities round about untoJerusalembringing sick folksand them which were vexed withunclean spiritsand they were healed every one.
17 Then the high priest rose upand all they that were with him which is the sect of the Sadducees and were filled withindignation
18 And laid their hands on the apostlesand put them in thecommon prison.
19 But the angel of the Lord by night opened the prison doorsand brought them forthand said
20 Gostand and speak in the temple to the people all the wordsof this life.
21 And when they heard thatthey entered into the temple earlyin the morningand taught. But the high priest cameand theythat were with himand called the council togetherand allthe senate of the children of Israeland sent to the prisonto have them brought.
22 But when the officers cameand found them not in the prison they returned and told
23 SayingThe prison truly found we shut with all safetyandthe keepers standing without before the doorsbut when we hadopenedwe found no man within.
24 Now when the high priest and the captain of the temple and thechief priests heard these thingsthey doubted of themwhereunto this would grow.
25 Then came one and told themsayingBeholdthe men whom yeput in prison are standing in the templeand teaching thepeople.
26 Then went the captain with the officersand brought themwithout violencefor they feared the peoplelest they shouldhave been stoned.
27 And when they had brought themthey set them before thecounciland the high priest asked them
28 SayingDid not we straitly command you that ye should notteach in this nameandbeholdye have filled Jerusalem withyour doctrineand intend to bring this man s blood upon us.
29 Then Peter and the other apostles answered and saidWe oughtto obey God rather than men.
30 The God of our fathers raised up Jesuswhom ye slew andhanged on a tree.
31 Him hath God exalted with his right hand to be a Prince and aSaviourfor to give repentance to Israeland forgiveness ofsins.
32 And we are his witnesses of these thingsand so is also theHoly Ghostwhom God hath given to them that obey him.
33 When they heard thatthey were cut to the heartand tookcounsel to slay them.
34 Then stood there up one in the councila PhariseenamedGamaliela doctor of the lawhad in reputation among all thepeopleand commanded to put the apostles forth a littlespace
35 And said unto themYe men of Israeltake heed to yourselveswhat ye intend to do as touching these men.
36 For before these days rose up Theudasboasting himself to besomebodyto whom a number of menabout four hundredjoinedthemselveswho was slainand allas many as obeyed him were scatteredand brought to nought.
37 After this man rose up Judas of Galilee in the days of thetaxingand drew away much people after himhe also perishedand alleven as many as obeyed himwere dispersed.
38 And now I say unto youRefrain from these menand let themalonefor if this counsel or this work be of menit willcome to nought
39 But if it be of Godye cannot overthrow itlest haply ye befound even to fight against God.
40 And to him they agreedand when they had called the apostles and beaten themthey commanded that they should not speak inthe name of Jesusand let them go.
41 And they departed from the presence of the councilrejoicingthat they were counted worthy to suffer shame for his name.
42 And daily in the templeand in every housethey ceased notto teach and preach Jesus Christ.

domain: literature


text:
Spatial variation of benthic macroinvertebrate biodiversity in boreal streams : biogeographic context and conservation implications
DisciplineLimnologia ja hydrobiologia
Biodiversity patterns of most lotic animal and plant groups remain poorly known, which hinders the implementation of effective conservation, monitoring, and restoration programs. The major aims of this thesis were (i) to examine spatial variation in macroinvertebrate assemblage structure and diversity across natural environmental gradients, and (ii) to assess the utility of such information as the basis of stream conservation programs.
PublisherUniversity of Jyväskylä
MetadataShow full item record
- Väitöskirjat
Showing items with similar title or keywords.
Kärnä, Olli-Matti; Heino, Jani; Laamanen, Tiina; Jyrkänkallio-Mikkola, Jenny; Pajunen, Virpi; Soininen, Janne; Tolonen, Kimmo T.; Tukiainen, Helena; Hjort, Jan (Springer Netherlands, 2019)Context One approach to maintain the resilience of biotic communities is to protect the variability of abiotic characteristics of Earth’s surface, i.e. geodiversity. In terrestrial environments, the relationship between ...
Purhonen, Jenna (Jyväskylän yliopisto, 2018)Dead wood and associated fungal communities are a crucial part of boreal forest ecosystems, and severely affected and threatened by human actions like commercial timber harvesting. Despite their importance for forest ...
Mazziotta, Adriano (University of Jyväskylä, 2014)
Do small protected habitat patches within boreal production forests provide value for biodiversity conservation? : A systematic review protocol Häkkilä, Matti; Savilaakso, Sini; Johansson, Anna; Sandgren, Terhi; Uusitalo, Anne; Mönkkönen, Mikko; Puttonen, Pasi (BioMed Central Ltd., 2019)Background Forest harvesting is the main driver of habitat degradation and biodiversity loss in forests of the boreal zone. To mitigate harmful effects, small-scale habitats with high biodiversity values have been protected ...
Environmental drivers of lake profundal macroinvertebrate community variation : implications for bioassessment Jyväsjärvi, Jussi (University of Jyväskylä, 2011)


text:
Purchase this article with an account.
Monique D Courtenay, William Cade, Gaofeng Wang, Stephen G Schwartz, Jaclyn L Kovach, Anita Agarwal, Milam A Brantley, William K Scott, Jonathan L Haines, Margaret A Pericak-Vance; Known Age-Related Macular Degeneration Risk Variants Are Not Associated with Rapid Disease Progression or Good Treatment Response. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):797.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
AMD is the leading cause of irreversible vision loss among older adults in developed countries. Genome-wide association meta-analysis has implicated common variations in 19 genes as AMD risk factors. Given the strong genetic influence on the risk of developing AMD, and the substantial variation in progression to advanced AMD and in treatment response, we investigated whether these clinical endpoints are also influenced by the known genetic variation.
In an existing dataset, 16 of the 19 GWAS loci were used to calculate a genetic risk score (summed number of risk alleles weighted by effect sizes) for each individual as outlined in Fritsche et al., 2013. To maximize our power for discovery, we created two “clinically extreme” case/control datasets with the following characteristics: 1) Rapid clinical progression of 36 individuals with early AMD or geographic atrophy to neovascular AMD within one year vs. 31 early AMD patients who did not progress over five or more years, and 2) 20 patients with bilateral neovascular AMD who were treated with intravitreal injections of anti-angiogenic agents for one year and demonstrated bilateral good treatment response (three lines of vision gain on the Snellen vision chart, prolonged or persistent (at least 6 months) absence of subretinal fluid (SRF) and absence of intraretinal fluid (IRF) on OCT, presence of unchanged small intraretinal cysts with no recurrence of SRF) vs. 22 neovascular AMD patients who demonstrated a poor response in one or both eyes (three lines of vision loss, persistent SRF and IRF, formation of disciform scar). The genetic risk score was tested for association with rapid progression or good treatment response with logistic regression adjusting for age and sex.
Risk score was not associated in either dataset in our study (progression P=0.43 and treatment P=0.63). Therefore, the genetic effects of the 19 known AMD risk loci are not associated with rapid progression or good treatment response in this small dataset, but perhaps a larger sample size would have sufficient power to demonstrate an effect.
Our results suggest that unknown genetic factors may still be regulating variability in progression and treatment response. Whole exome sequencing is currently underway in this dataset to test if novel rare or common variants are associated with these clinical endpoints.
This PDF is available to Subscribers Only


text:
Are you looking for a how to win at gambling, tutorial to casino guidebook? Several people that are new to modern casino video games haven’t any basic idea how exactly to pick the greatest gambling house games. Of course there are many choices with regards to casinos, but it is focused on knowing those provide greatest bonuses and odds. Here’s how to win at gambling, guide to casino.
If you intend to win on the casino, you then must look for a casino where you are more prone to come apart with a positive cash pay off. Before you can see how to win at playing, guide to casino, ensure that you are familiar with your card game preferences.
If you have any sort of inquiries regarding where and ways to use Online Casino Malaysia https://i8win.net, you can contact us at our page.
Credit card game interests are usually the very first thing to take in considering the way to play a gambling house game. For example, if you are an avid participant of blackjack, after that blackjack may be the sport that you’ll want to play probably. There are also popular casino cards such as for example craps and roulette. If you’re thinking about playing a particular casino game, then you might want to concentrate on that game.
If you are not a blackjack or craps fan, you’ll be able to always focus on something else as your first casino game. For example, if you’re into sports, then the best way to learn how to win at gambling, guide to casino is to find a casino where the most popular game is baseball. Quite simply, if you like baseball, you need to look for the best football casino then.
In fact, if you’re after a how to win at gambling, guide to casino, then you have the decision to either focus on a casino game that’s like the one you are currently playing. For instance, if you want blackjack, then you might want to start playing roulette. For those who love sports, there are casino game with gambling on games such as football furthermore. However, if you don’t really know what to do, then you may desire to just choose a casino game that you will be familiar with.
The next thing to consider if you are buying how exactly to win at gambling, guide to casino may be the location of the casino. Again, blackjack casino, after that you need to choose one that offers a lot of blackjack participants in it. For example, if there are a lot of players with plenty of blackjack experience within the casino, the odds will be much better after that.
Another thing you need to look at may be the gambling environment within the casino. If the place is usually as well loud, then you may not be able to perform at your best. So you have to make sure that the internet casino offers privacy for you to exercise sufficiently.
Also, if the casino provides a good variety of casino games, then it means you could bet on many different types of games. You must pick the best among the choices provided by the casino. You can then try out the different games and see which one works best for you personally.
The solution to win at playing, guide to internet casino is also dependent on your character. If you’re the sort of person who will not like to bet too much, then you would have to make sure that you get your fair share of bets from your casino. This can not only help you in determining how to win at playing, guide to modern casino, but maintain you pleased furthermore.
Remember that whenever it comes to playing a casino game, if you arenot excited too, then you will lose. And you will not win. Therefore select a sport that’s not interesting as well.
After you have found a casino game that is not too exciting, you then should try to learn to win at gambling, guide to casino by practicing at it for some time. so that you shall not get dissatisfied in the event that you get rid of.
When you loved this informative article and you would like to receive more info concerning Trusted Online Casino Malaysia assure visit our own web site.
If you were enthusiastic about the material on this short article, here are several additional pages and posts with a similar material:

domain: game


text:
With college football kicking off tonight, many sports fans in the United States are unsure of the status of sports betting within their state.
A guy wants to put fifty bucks from his hard-earned paycheck on his favorite team and he doesn’t know if he is allowed or not. Freedom.
The good news is that many states are making the move to allow sports betting in some fashion within their state. The ultimate experience, of course, would be if each and every state had multiple mobile betting options.
Some in the industry believe that most states WILL have multiple mobile betting options within five years time. Of course, there will be some hold out states that traditionally limit the freedom of their residents.
This map shows every state within the United States and its current position on sports betting: click to enlarge

domain: geography


text:
Concussion is one of the most commonly reported injuries amongst children and youth involved in sport participation. Following a concussion, youth can experience a range of short and long term neurobehavioral symptoms (somatic, cognitive and emotional/behavioral) that can have a significant impact on one’s participation in daily activities and pursuits of interest (e.g., school, sports, work, family/social life, etc.). Despite this, there remains a paucity in clinically driven research aimed specifically at exploring concussion within the youth sport population, and more specifically, multi-modal approaches to measuring recovery. This article provides an overview of a novel and multi-modal approach to measuring recovery amongst youth athletes following concussion. The presented approach involves the use of both pre-injury/baseline testing and post-injury/follow-up testing to assess performance across a wide variety of domains (post-concussion symptoms, cognition, balance, strength, agility/motor skills and resting state heart rate variability). The goal of this research is to gain a more objective and accurate understanding of recovery following concussion in youth athletes (ages 10-18 years). Findings from this research can help to inform the development and use of improved approaches to concussion management and rehabilitation specific to the youth sport community.
24 Related JoVE Articles!
Bronchial Thermoplasty: A Novel Therapeutic Approach to Severe Asthma
Institutions: Virginia Hospital Center, Virginia Hospital Center.
Bronchial thermoplasty is a non-drug procedure for severe persistent asthma that delivers thermal energy to the airway wall in a precisely controlled manner to reduce excessive airway smooth muscle. Reducing airway smooth muscle decreases the ability of the airways to constrict, thereby reducing the frequency of asthma attacks. Bronchial thermoplasty is delivered by the Alair System and is performed in three outpatient procedure visits, each scheduled approximately three weeks apart. The first procedure treats the airways of the right lower lobe, the second treats the airways of the left lower lobe and the third and final procedure treats the airways in both upper lobes. After all three procedures are performed the bronchial thermoplasty treatment is complete.
Bronchial thermoplasty is performed during bronchoscopy with the patient under moderate sedation. All accessible airways distal to the mainstem bronchi between 3 and 10 mm in diameter, with the exception of the right middle lobe, are treated under bronchoscopic visualization. Contiguous and non-overlapping activations of the device are used, moving from distal to proximal along the length of the airway, and systematically from airway to airway as described previously. Although conceptually straightforward, the actual execution of bronchial thermoplasty is quite intricate and procedural duration for the treatment of a single lobe is often substantially longer than encountered during routine bronchoscopy. As such, bronchial thermoplasty should be considered a complex interventional bronchoscopy and is intended for the experienced bronchoscopist. Optimal patient management is critical in any such complex and longer duration bronchoscopic procedure. This article discusses the importance of careful patient selection, patient preparation, patient management, procedure duration, postoperative care and follow-up to ensure that bronchial thermoplasty is performed safely.
Bronchial thermoplasty is expected to complement asthma maintenance medications by providing long-lasting asthma control and improving asthma-related quality of life of patients with severe asthma. In addition, bronchial thermoplasty has been demonstrated to reduce severe exacerbations (asthma attacks) emergency rooms visits for respiratory symptoms, and time lost from work, school and other daily activities due to asthma.
Medicine, Issue 45, bronchial thermoplasty, severe asthma, airway smooth muscle, bronchoscopy, radiofrequency energy, patient management, moderate sedation
Thermal Ablation for the Treatment of Abdominal Tumors
Institutions: University of Wisconsin-Madison, University of Wisconsin-Madison.
Percutaneous thermal ablation is an emerging treatment option for many tumors of the abdomen not amenable to conventional treatments. During a thermal ablation procedure, a thin applicator is guided into the target tumor under imaging guidance. Energy is then applied to the tissue until temperatures rise to cytotoxic levels (50-60 °C). Various energy sources are available to heat biological tissues, including radiofrequency (RF) electrical current, microwaves, laser light and ultrasonic waves. Of these, RF and microwave ablation are most commonly used worldwide.
During RF ablation, alternating electrical current (~500 kHz) produces resistive heating around the interstitial electrode. Skin surface electrodes (ground pads) are used to complete the electrical circuit. RF ablation has been in use for nearly 20 years, with good results for local tumor control, extended survival and low complication rates1,2
. Recent studies suggest RF ablation may be a first-line treatment option for small hepatocellular carcinoma and renal-cell carcinoma3-5
. However, RF heating is hampered by local blood flow and high electrical impedance tissues (eg, lung, bone, desiccated or charred tissue)6,7
. Microwaves may alleviate some of these problems by producing faster, volumetric heating8-10
. To create larger or conformal ablations, multiple microwave antennas can be used simultaneously while RF electrodes require sequential operation, which limits their efficiency. Early experiences with microwave systems suggest efficacy and safety similar to, or better than RF devices11-13
Alternatively, cryoablation freezes the target tissues to lethal levels (-20 to -40 °C). Percutaneous cryoablation has been shown to be effective against RCC and many metastatic tumors, particularly colorectal cancer, in the liver14-16
. Cryoablation may also be associated with less post-procedure pain and faster recovery for some indications17
. Cryoablation is often contraindicated for primary liver cancer due to underlying coagulopathy and associated bleeding risks frequently seen in cirrhotic patients. In addition, sudden release of tumor cellular contents when the frozen tissue thaws can lead to a potentially serious condition known as cryoshock 16
Thermal tumor ablation can be performed at open surgery, laparoscopy or using a percutaneous approach. When performed percutaneously, the ablation procedure relies on imaging for diagnosis, planning, applicator guidance, treatment monitoring and follow-up. Ultrasound is the most popular modality for guidance and treatment monitoring worldwide, but computed tomography (CT) and magnetic resonance imaging (MRI) are commonly used as well. Contrast-enhanced CT or MRI are typically employed for diagnosis and follow-up imaging.
Medicine, Issue 49, Thermal ablation, interventional oncology, image-guided therapy, radiology, cancer
Surgical Technique for Spinal Cord Delivery of Therapies: Demonstration of Procedure in Gottingen Minipigs
Institutions: Emory University, Medical University of South Carolina, University of Alabama, Birmingham, Georgia Institute of Technology , Emory University.
This is a compact visual description of a combination of surgical technique and device for the delivery of (gene and cell) therapies into the spinal cord. While the technique is demonstrated in the animal, the procedure is FDA-approved and currently being used for stem cell transplantation into the spinal cords of patients with ALS. While the FDA has recognized proof-of-principle data on therapeutic efficacy in highly characterized rodent models, the use of large animals is considered critical for validating the combination of a surgical procedure, a device, and the safety of a final therapy for human use. The size, anatomy, and general vulnerability of the spine and spinal cord of the swine are recognized to better model the human. Moreover, the surgical process of exposing and manipulating the spinal cord as well as closing the wound in the pig is virtually indistinguishable from the human. We believe that the healthy pig model represents a critical first step in the study of procedural safety.
Medicine, Issue 70, Neuroscience, Neurobiology, Anatomy, Physiology, Surgery, accuracy, delivery, safety, spinal cord, CNS, target, therapy, transplantation, swine, animal model
Assessment of Cardiac Function and Myocardial Morphology Using Small Animal Look-locker Inversion Recovery (SALLI) MRI in Rats
Institutions: German Heart Institute Berlin, German Heart Institute Berlin, Hamburg, Germany.
Small animal magnetic resonance imaging is an important tool to study cardiac function and changes in myocardial tissue. The high heart rates of small animals (200 to 600 beats/min) have previously limited the role of CMR imaging. Small animal Look-Locker inversion recovery (SALLI) is a T1 mapping sequence for small animals to overcome this problem 1
. T1 maps provide quantitative information about tissue alterations and contrast agent kinetics. It is also possible to detect diffuse myocardial processes such as interstitial fibrosis or edema 1-6
. Furthermore, from a single set of image data, it is possible to examine heart function and myocardial scarring by generating cine and inversion recovery-prepared late gadolinium enhancement-type MR images 1
The presented video shows step-by-step the procedures to perform small animal CMR imaging. Here it is presented with a healthy Sprague-Dawley rat, however naturally it can be extended to different cardiac small animal models.
Medicine, Issue 77, Biomedical Engineering, Anatomy, Physiology, Cardiology, Heart Diseases, Cardiomyopathies, Heart Failure, Diagnostic Imaging, Cardiac Imaging Techniques, Magnetic Resonance Imaging, MRI, Cardiovascular Diseases, small animal imaging, T1 mapping, heart disease, cardiac function, myocardium, rat, animal model
Assessment and Evaluation of the High Risk Neonate: The NICU Network Neurobehavioral Scale
Institutions: Brown University, Women & Infants Hospital of Rhode Island, University of Massachusetts, Boston.
There has been a long-standing interest in the assessment of the neurobehavioral integrity of the newborn infant. The NICU Network Neurobehavioral Scale (NNNS) was developed as an assessment for the at-risk infant. These are infants who are at increased risk for poor developmental outcome because of insults during prenatal development, such as substance exposure or prematurity or factors such as poverty, poor nutrition or lack of prenatal care that can have adverse effects on the intrauterine environment and affect the developing fetus. The NNNS assesses the full range of infant neurobehavioral performance including neurological integrity, behavioral functioning, and signs of stress/abstinence. The NNNS is a noninvasive neonatal assessment tool with demonstrated validity as a predictor, not only of medical outcomes such as cerebral palsy diagnosis, neurological abnormalities, and diseases with risks to the brain, but also of developmental outcomes such as mental and motor functioning, behavior problems, school readiness, and IQ. The NNNS can identify infants at high risk for abnormal developmental outcome and is an important clinical tool that enables medical researchers and health practitioners to identify these infants and develop intervention programs to optimize the development of these infants as early as possible. The video shows the NNNS procedures, shows examples of normal and abnormal performance and the various clinical populations in which the exam can be used.
Behavior, Issue 90, NICU Network Neurobehavioral Scale, NNNS, High risk infant, Assessment, Evaluation, Prediction, Long term outcome
Development of a Virtual Reality Assessment of Everyday Living Skills
Institutions: NeuroCog Trials, Inc., Duke-NUS Graduate Medical Center, Duke University Medical Center, Fox Evaluation and Consulting, PLLC, University of Miami Miller School of Medicine.
Cognitive impairments affect the majority of patients with schizophrenia and these impairments predict poor long term psychosocial outcomes. Treatment studies aimed at cognitive impairment in patients with schizophrenia not only require demonstration of improvements on cognitive tests, but also evidence that any cognitive changes lead to clinically meaningful improvements. Measures of “functional capacity” index the extent to which individuals have the potential to perform skills required for real world functioning. Current data do not support the recommendation of any single instrument for measurement of functional capacity. The Virtual Reality Functional Capacity Assessment Tool (VRFCAT) is a novel, interactive gaming based measure of functional capacity that uses a realistic simulated environment to recreate routine activities of daily living. Studies are currently underway to evaluate and establish the VRFCAT’s sensitivity, reliability, validity, and practicality. This new measure of functional capacity is practical, relevant, easy to use, and has several features that improve validity and sensitivity of measurement of function in clinical trials of patients with CNS disorders.
Behavior, Issue 86, Virtual Reality, Cognitive Assessment, Functional Capacity, Computer Based Assessment, Schizophrenia, Neuropsychology, Aging, Dementia
Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
Institutions: LSU Health Sciences Center, University of Milan.
MicroRNAs (miRNAs) constitute a potent layer of gene regulation by guiding RISC to target sites located on mRNAs and, consequently, by modulating their translational repression. Changes in miRNA expression have been shown to be involved in the development of all major complex diseases. Furthermore, recent findings showed that miRNAs can be secreted to the extracellular environment and enter the bloodstream and other body fluids where they can circulate with high stability. The function of such circulating miRNAs remains largely elusive, but systematic high throughput approaches, such as miRNA profiling arrays, have lead to the identification of miRNA signatures in several pathological conditions, including neurodegenerative disorders and several types of cancers. In this context, the identification of miRNA expression profile in the cerebrospinal fluid, as reported in our recent study, makes miRNAs attractive candidates for biomarker analysis.
There are several tools available for profiling microRNAs, such as microarrays, quantitative real-time PCR (qPCR), and deep sequencing. Here, we describe a sensitive method to profile microRNAs in cerebrospinal fluids by quantitative real-time PCR. We used the Exiqon microRNA ready-to-use PCR human panels I and II V2.R, which allows detection of 742 unique human microRNAs. We performed the arrays in triplicate runs and we processed and analyzed data using the GenEx Professional 5 software.
Using this protocol, we have successfully profiled microRNAs in various types of cell lines and primary cells, CSF, plasma, and formalin-fixed paraffin-embedded tissues.
Medicine, Issue 83, microRNAs, biomarkers, miRNA profiling, qPCR, cerebrospinal fluid, RNA, DNA
Habituation and Prepulse Inhibition of Acoustic Startle in Rodents
Institutions: University of Western Ontario.
The acoustic startle response is a protective response, elicited by a sudden and intense acoustic stimulus. Facial and skeletal muscles are activated within a few milliseconds, leading to a whole body flinch in rodents1
. Although startle responses are reflexive responses that can be reliably elicited, they are not stereotypic. They can be modulated by emotions such as fear (fear potentiated startle) and joy (joy attenuated startle), by non-associative learning processes such as habituation and sensitization, and by other sensory stimuli through sensory gating processes (prepulse inhibition), turning startle responses into an excellent tool for assessing emotions, learning, and sensory gating, for review see 2, 3
. The primary pathway mediating startle responses is very short and well described, qualifying startle also as an excellent model for studying the underlying mechanisms for behavioural plasticity on a cellular/molecular level3
We here describe a method for assessing short-term habituation, long-term habituation and prepulse inhibition of acoustic startle responses in rodents. Habituation describes the decrease of the startle response magnitude upon repeated presentation of the same stimulus. Habituation within a testing session is called short-term habituation (STH) and is reversible upon a period of several minutes without stimulation. Habituation between testing sessions is called long-term habituation (LTH)4
. Habituation is stimulus specific5
. Prepulse inhibition is the attenuation of a startle response by a preceding non-startling sensory stimulus6
. The interval between prepulse and startle stimulus can vary from 6 to up to 2000 ms. The prepulse can be any modality, however, acoustic prepulses are the most commonly used.
Habituation is a form of non-associative learning. It can also be viewed as a form of sensory filtering, since it reduces the organisms' response to a non-threatening stimulus. Prepulse inhibition (PPI) was originally developed in human neuropsychiatric research as an operational measure for sensory gating7
. PPI deficits may represent the interface of "psychosis and cognition" as they seem to predict cognitive impairment8-10
. Both habituation and PPI are disrupted in patients suffering from schizophrenia11
, and PPI disruptions have shown to be, at least in some cases, amenable to treatment with mostly atypical antipsychotics12, 13
. However, other mental and neurodegenerative diseases are also accompanied by disruption in habituation and/or PPI, such as autism spectrum disorders (slower habituation), obsessive compulsive disorder, Tourette's syndrome, Huntington's disease, Parkinson's disease, and Alzheimer's Disease (PPI)11, 14, 15
Dopamine induced PPI deficits are a commonly used animal model for the screening of antipsychotic drugs16
, but PPI deficits can also be induced by many other psychomimetic drugs, environmental modifications and surgical procedures.
Neuroscience, Issue 55, Startle responses, rat, mouse, sensory gating, sensory filtering, short-term habituation, long-term habituation, prepulse inhibition
A Neuroscientific Approach to the Examination of Concussions in Student-Athletes
Institutions: Elon University, Elon University, Duquesne University, Elon University.
Concussions are occurring at alarming rates in the United States and have become a serious public health concern. The CDC estimates that 1.6 to 3.8 million concussions occur in sports and recreational activities annually. Concussion as defined by the 2013 Concussion Consensus Statement “may be caused either by a direct blow to the head, face, neck or elsewhere on the body with an ‘impulsive’ force transmitted to the head.” Concussions leave the individual with both short- and long-term effects. The short-term effects of sport related concussions may include changes in playing ability, confusion, memory disturbance, the loss of consciousness, slowing of reaction time, loss of coordination, headaches, dizziness, vomiting, changes in sleep patterns and mood changes. These symptoms typically resolve in a matter of days. However, while some individuals recover from a single concussion rather quickly, many experience lingering effects that can last for weeks or months. The factors related to concussion susceptibility and the subsequent recovery times are not well known or understood at this time. Several factors have been suggested and they include the individual’s concussion history, the severity of the initial injury, history of migraines, history of learning disabilities, history of psychiatric comorbidities, and possibly, genetic factors. Many studies have individually investigated certain factors both the short-term and long-term effects of concussions, recovery time course, susceptibility and recovery. What has not been clearly established is an effective multifaceted approach to concussion evaluation that would yield valuable information related to the etiology, functional changes, and recovery. The purpose of this manuscript is to show one such multifaceted approached which examines concussions using computerized neurocognitive testing, event related potentials, somatosensory perceptual responses, balance assessment, gait assessment and genetic testing.
Medicine, Issue 94, Concussions, Student-Athletes, Mild Traumatic Brain Injury, Genetics, Cognitive Function, Balance, Gait, Somatosensory
Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
Institutions: UT Health Science Center at San Antonio, UT Health Science Center at San Antonio, UT Health Science Center at San Antonio, UT Health Science Center at San Antonio, UT Health Science Center at San Antonio.
In recent years, it has become apparent that genomic instability is tightly related to many developmental disorders, cancers, and aging. Given that stem cells are responsible for ensuring tissue homeostasis and repair throughout life, it is reasonable to hypothesize that the stem cell population is critical for preserving genomic integrity of tissues. Therefore, significant interest has arisen in assessing the impact of endogenous and environmental factors on genomic integrity in stem cells and their progeny, aiming to understand the etiology of stem-cell based diseases.
transgenic mice carry a recoverable λ phage vector encoding the LacI
reporter system, in which the LacI
gene serves as the mutation reporter. The result of a mutated LacI
gene is the production of β-galactosidase that cleaves a chromogenic substrate, turning it blue. The LacI
reporter system is carried in all cells, including stem/progenitor cells and can easily be recovered and used to subsequently infect E. coli
. After incubating infected E. coli
on agarose that contains the correct substrate, plaques can be scored; blue plaques indicate a mutant LacI
gene, while clear plaques harbor wild-type. The frequency of blue (among clear) plaques indicates the mutant frequency in the original cell population the DNA was extracted from. Sequencing the mutant LacI
gene will show the location of the mutations in the gene and the type of mutation.
transgenic mouse model is well-established as an in vivo
mutagenesis assay. Moreover, the mice and the reagents for the assay are commercially available. Here we describe in detail how this model can be adapted to measure the frequency of spontaneously occurring DNA mutants in stem cell-enriched Lin-
(LSK) cells and other subpopulations of the hematopoietic system.
Infection, Issue 84, In vivo mutagenesis, hematopoietic stem/progenitor cells, LacI mouse model, DNA mutations, E. coli
Breathing-controlled Electrical Stimulation (BreEStim) for Management of Neuropathic Pain and Spasticity
Institutions: University of Texas Health Science Center at Houston , TIRR Memorial Hermann Hospital, TIRR Memorial Hermann Hospital.
Electrical stimulation (EStim) refers to the application of electrical current to muscles or nerves in order to achieve functional and therapeutic goals. It has been extensively used in various clinical settings. Based upon recent discoveries related to the systemic effects of voluntary breathing and intrinsic physiological interactions among systems during voluntary breathing, a new EStim protocol, Breathing-controlled Electrical Stimulation (BreEStim), has been developed to augment the effects of electrical stimulation. In BreEStim, a single-pulse electrical stimulus is triggered and delivered to the target area when the airflow rate of an isolated voluntary inspiration reaches the threshold. BreEStim integrates intrinsic physiological interactions that are activated during voluntary breathing and has demonstrated excellent clinical efficacy. Two representative applications of BreEStim are reported with detailed protocols: management of post-stroke finger flexor spasticity and neuropathic pain in spinal cord injury.
Medicine, Issue 71, Neuroscience, Neurobiology, Anatomy, Physiology, Behavior, electrical stimulation, BreEStim, electrode, voluntary breathing, respiration, inspiration, pain, neuropathic pain, pain management, spasticity, stroke, spinal cord injury, brain, central nervous system, CNS, clinical, electromyogram, neuromuscular electrical stimulation
Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney
Institutions: University of Notre Dame.
The zebrafish model has emerged as a relevant system to study kidney development, regeneration and disease. Both the embryonic and adult zebrafish kidneys are composed of functional units known as nephrons, which are highly conserved with other vertebrates, including mammals. Research in zebrafish has recently demonstrated that two distinctive phenomena transpire after adult nephrons incur damage: first, there is robust regeneration within existing nephrons that replaces the destroyed tubule epithelial cells; second, entirely new nephrons are produced from renal progenitors in a process known as neonephrogenesis. In contrast, humans and other mammals seem to have only a limited ability for nephron epithelial regeneration. To date, the mechanisms responsible for these kidney regeneration phenomena remain poorly understood. Since adult zebrafish kidneys undergo both nephron epithelial regeneration and neonephrogenesis, they provide an outstanding experimental paradigm to study these events. Further, there is a wide range of genetic and pharmacological tools available in the zebrafish model that can be used to delineate the cellular and molecular mechanisms that regulate renal regeneration. One essential aspect of such research is the evaluation of nephron structure and function. This protocol describes a set of labeling techniques that can be used to gauge renal composition and test nephron functionality in the adult zebrafish kidney. Thus, these methods are widely applicable to the future phenotypic characterization of adult zebrafish kidney injury paradigms, which include but are not limited to, nephrotoxicant exposure regimes or genetic methods of targeted cell death such as the nitroreductase mediated cell ablation technique. Further, these methods could be used to study genetic perturbations in adult kidney formation and could also be applied to assess renal status during chronic disease modeling.
Cellular Biology, Issue 90,
zebrafish; kidney; nephron; nephrology; renal; regeneration; proximal tubule; distal tubule; segment; mesonephros; physiology; acute kidney injury (AKI)
The Use of Magnetic Resonance Spectroscopy as a Tool for the Measurement of Bi-hemispheric Transcranial Electric Stimulation Effects on Primary Motor Cortex Metabolism
Institutions: University of Montréal, McGill University, University of Minnesota.
Transcranial direct current stimulation (tDCS) is a neuromodulation technique that has been increasingly used over the past decade in the treatment of neurological and psychiatric disorders such as stroke and depression. Yet, the mechanisms underlying its ability to modulate brain excitability to improve clinical symptoms remains poorly understood 33
. To help improve this understanding, proton magnetic resonance spectroscopy (1
H-MRS) can be used as it allows the in vivo
quantification of brain metabolites such as γ-aminobutyric acid (GABA) and glutamate in a region-specific manner 41
. In fact, a recent study demonstrated that 1
H-MRS is indeed a powerful means to better understand the effects of tDCS on neurotransmitter concentration 34
. This article aims to describe the complete protocol for combining tDCS (NeuroConn MR compatible stimulator) with 1
H-MRS at 3 T using a MEGA-PRESS sequence. We will describe the impact of a protocol that has shown great promise for the treatment of motor dysfunctions after stroke, which consists of bilateral stimulation of primary motor cortices 27,30,31
. Methodological factors to consider and possible modifications to the protocol are also discussed.
Neuroscience, Issue 93, proton magnetic resonance spectroscopy, transcranial direct current stimulation, primary motor cortex, GABA, glutamate, stroke
Tissue Triage and Freezing for Models of Skeletal Muscle Disease
Institutions: Medical College of Wisconsin, The Ohio State University, Virginia Tech, University of Kentucky, Boston Children's Hospital, Harvard Medical School, Cure Congenital Muscular Dystrophy, Joshua Frase Foundation, University of Washington, University of Arizona.
Skeletal muscle is a unique tissue because of its structure and function, which requires specific protocols for tissue collection to obtain optimal results from functional, cellular, molecular, and pathological evaluations. Due to the subtlety of some pathological abnormalities seen in congenital muscle disorders and the potential for fixation to interfere with the recognition of these features, pathological evaluation of frozen muscle is preferable to fixed muscle when evaluating skeletal muscle for congenital muscle disease. Additionally, the potential to produce severe freezing artifacts in muscle requires specific precautions when freezing skeletal muscle for histological examination that are not commonly used when freezing other tissues. This manuscript describes a protocol for rapid freezing of skeletal muscle using isopentane (2-methylbutane) cooled with liquid nitrogen to preserve optimal skeletal muscle morphology. This procedure is also effective for freezing tissue intended for genetic or protein expression studies. Furthermore, we have integrated our freezing protocol into a broader procedure that also describes preferred methods for the short term triage of tissue for (1) single fiber functional studies and (2) myoblast cell culture, with a focus on the minimum effort necessary to collect tissue and transport it to specialized research or reference labs to complete these studies. Overall, this manuscript provides an outline of how fresh tissue can be effectively distributed for a variety of phenotypic studies and thereby provides standard operating procedures (SOPs) for pathological studies related to congenital muscle disease.
Basic Protocol, Issue 89,
Tissue, Freezing, Muscle, Isopentane, Pathology, Functional Testing, Cell Culture
A Novel Method for Assessing Proximal and Distal Forelimb Function in the Rat: the Irvine, Beatties and Bresnahan (IBB) Forelimb Scale
Institutions: University of California, San Francisco.
Several experimental models of cervical spinal cord injury (SCI) have been developed recently to assess the consequences of damage to this level of the spinal cord (Pearse et al.
, 2005, Gensel et al.
, 2006, Anderson et al.
, 2009), as the majority of human SCI occur here (Young, 2010; www.sci-info-pages.com). Behavioral deficits include loss of forelimb function due to damage to the white matter affecting both descending motor and ascending sensory systems, and to the gray matter containing the segmental circuitry for processing sensory input and motor output for the forelimb. Additionally, a key priority for human patients with cervical SCI is restoration of hand/arm function (Anderson, 2004). Thus, outcome measures that assess both proximal and distal forelimb function are needed. Although there are several behavioral assays that are sensitive to different aspects of forelimb recovery in experimental models of cervical SCI (Girgis et al.
, 2007, Gensel et al.
, 2006, Ballerman et al.
, 2001, Metz and Whishaw, 2000, Bertelli and Mira, 1993, Montoya et al.
, 1991, Whishaw and Pellis, 1990), few techniques provide detailed information on the recovery of fine motor control and digit movement.
The current measurement technique, the Irvine, Beatties and Bresnahan forelimb scale (IBB), can detect recovery of both proximal and distal forelimb function including digit movements during a naturally occurring behavior that does not require extensive training or deprivation to enhance motivation. The IBB was generated by observing recovery after a unilateral C6 SCI, and involves video recording of animals eating two differently shaped cereals (spherical and doughnut) of a consistent size. These videos were then used to assess features of forelimb use, such as joint position, object support, digit movement and grasping technique.
The IBB, like other forelimb behavioral tasks, shows a consistent pattern of recovery that is sensitive to injury severity. Furthermore, the IBB scale could be used to assess recovery following other types of injury that impact normal forelimb function.
Neuroscience, Issue 46, spinal cord injury, recovery of function, forelimb function, neurological test, cervical injuries
Isolation and Quantification of Botulinum Neurotoxin From Complex Matrices Using the BoTest Matrix Assays
Institutions: BioSentinel Inc., Madison, WI.
Accurate detection and quantification of botulinum neurotoxin (BoNT) in complex matrices is required for pharmaceutical, environmental, and food sample testing. Rapid BoNT testing of foodstuffs is needed during outbreak forensics, patient diagnosis, and food safety testing while accurate potency testing is required for BoNT-based drug product manufacturing and patient safety. The widely used mouse bioassay for BoNT testing is highly sensitive but lacks the precision and throughput needed for rapid and routine BoNT testing. Furthermore, the bioassay's use of animals has resulted in calls by drug product regulatory authorities and animal-rights proponents in the US and abroad to replace the mouse bioassay for BoNT testing. Several in vitro
replacement assays have been developed that work well with purified BoNT in simple buffers, but most have not been shown to be applicable to testing in highly complex matrices. Here, a protocol for the detection of BoNT in complex matrices using the BoTest Matrix assays is presented. The assay consists of three parts: The first part involves preparation of the samples for testing, the second part is an immunoprecipitation step using anti-BoNT antibody-coated paramagnetic beads to purify BoNT from the matrix, and the third part quantifies the isolated BoNT's proteolytic activity using a fluorogenic reporter. The protocol is written for high throughput testing in 96-well plates using both liquid and solid matrices and requires about 2 hr of manual preparation with total assay times of 4-26 hr depending on the sample type, toxin load, and desired sensitivity. Data are presented for BoNT/A testing with phosphate-buffered saline, a drug product, culture supernatant, 2% milk, and fresh tomatoes and includes discussion of critical parameters for assay success.
Neuroscience, Issue 85, Botulinum, food testing, detection, quantification, complex matrices, BoTest Matrix, Clostridium, potency testing
A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos
Institutions: University of Notre Dame.
Zebrafish have become a widely used model organism to investigate the mechanisms that underlie developmental biology and to study human disease pathology due to their considerable degree of genetic conservation with humans. Chemical genetics entails testing the effect that small molecules have on a biological process and is becoming a popular translational research method to identify therapeutic compounds. Zebrafish are specifically appealing to use for chemical genetics because of their ability to produce large clutches of transparent embryos, which are externally fertilized. Furthermore, zebrafish embryos can be easily drug treated by the simple addition of a compound to the embryo media. Using whole-mount in situ
hybridization (WISH), mRNA expression can be clearly visualized within zebrafish embryos. Together, using chemical genetics and WISH, the zebrafish becomes a potent whole organism context in which to determine the cellular and physiological effects of small molecules. Innovative advances have been made in technologies that utilize machine-based screening procedures, however for many labs such options are not accessible or remain cost-prohibitive. The protocol described here explains how to execute a manual high-throughput chemical genetic screen that requires basic resources and can be accomplished by a single individual or small team in an efficient period of time. Thus, this protocol provides a feasible strategy that can be implemented by research groups to perform chemical genetics in zebrafish, which can be useful for gaining fundamental insights into developmental processes, disease mechanisms, and to identify novel compounds and signaling pathways that have medically relevant applications.
Developmental Biology, Issue 93, zebrafish, chemical genetics, chemical screen, in vivo small molecule screen, drug discovery, whole mount in situ hybridization (WISH), high-throughput screening (HTS), high-content screening (HCS)
Community-based Adapted Tango Dancing for Individuals with Parkinson's Disease and Older Adults
Institutions: Emory University School of Medicine, Brigham and Woman‘s Hospital and Massachusetts General Hospital.
Adapted tango dancing improves mobility and balance in older adults and additional populations with balance impairments. It is composed of very simple step elements. Adapted tango involves movement initiation and cessation, multi-directional perturbations, varied speeds and rhythms. Focus on foot placement, whole body coordination, and attention to partner, path of movement, and aesthetics likely underlie adapted tango’s demonstrated efficacy for improving mobility and balance. In this paper, we describe the methodology to disseminate the adapted tango teaching methods to dance instructor trainees and to implement the adapted tango by the trainees in the community for older adults and individuals with Parkinson’s Disease (PD). Efficacy in improving mobility (measured with the Timed Up and Go, Tandem stance, Berg Balance Scale, Gait Speed and 30 sec chair stand), safety and fidelity of the program is maximized through targeted instructor and volunteer training and a structured detailed syllabus outlining class practices and progression.
Behavior, Issue 94, Dance, tango, balance, pedagogy, dissemination, exercise, older adults, Parkinson's Disease, mobility impairments, falls
Psychophysiological Stress Assessment Using Biofeedback
Institutions: Cambridge Health Alliance, Harvard Medical School.
In the last half century, research in biofeedback has shown the extent to which the human mind can influence the functioning of the autonomic nervous system, previously thought to be outside of conscious control. By letting people observe signals from their own bodies, biofeedback enables them to develop greater awareness of their physiological and psychological reactions, such as stress, and to learn to modify these reactions. Biofeedback practitioners can facilitate this process by assessing people s reactions to mildly stressful events and formulating a biofeedback-based treatment plan. During stress assessment the practitioner first records a baseline for physiological readings, and then presents the client with several mild stressors, such as a cognitive, physical and emotional stressor. Variety of stressors is presented in order to determine a person's stimulus-response specificity, or differences in each person's reaction to qualitatively different stimuli. This video will demonstrate the process of psychophysiological stress assessment using biofeedback and present general guidelines for treatment planning.
Neuroscience, Issue 29, Stress, biofeedback, psychophysiological, assessment
Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in Saccharomyces cerevisiae
Institutions: Rensselaer Polytechnic Institute.
has been an excellent model system for examining mechanisms and consequences of genome instability. Information gained from this yeast model is relevant to many organisms, including humans, since DNA repair and DNA damage response factors are well conserved across diverse species. However, S. cerevisiae
has not yet been used to fully address whether the rate of accumulating mutations changes with increasing replicative (mitotic) age due to technical constraints. For instance, measurements of yeast replicative lifespan through micromanipulation involve very small populations of cells, which prohibit detection of rare mutations. Genetic methods to enrich for mother cells in populations by inducing death of daughter cells have been developed, but population sizes are still limited by the frequency with which random mutations that compromise the selection systems occur. The current protocol takes advantage of magnetic sorting of surface-labeled yeast mother cells to obtain large enough populations of aging mother cells to quantify rare mutations through phenotypic selections. Mutation rates, measured through fluctuation tests, and mutation frequencies are first established for young cells and used to predict the frequency of mutations in mother cells of various replicative ages. Mutation frequencies are then determined for sorted mother cells, and the age of the mother cells is determined using flow cytometry by staining with a fluorescent reagent that detects bud scars formed on their cell surfaces during cell division. Comparison of predicted mutation frequencies based on the number of cell divisions to the frequencies experimentally observed for mother cells of a given replicative age can then identify whether there are age-related changes in the rate of accumulating mutations. Variations of this basic protocol provide the means to investigate the influence of alterations in specific gene functions or specific environmental conditions on mutation accumulation to address mechanisms underlying genome instability during replicative aging.
Microbiology, Issue 92, Aging, mutations, genome instability, Saccharomyces cerevisiae, fluctuation test, magnetic sorting, mother cell, replicative aging
Using Visual and Narrative Methods to Achieve Fair Process in Clinical Care
Institutions: Brandeis University, Brandeis University.
The Institute of Medicine has targeted patient-centeredness as an important area of quality improvement. A major dimension of patient-centeredness is respect for patient's values, preferences, and expressed needs. Yet specific approaches to gaining this understanding and translating it to quality care in the clinical setting are lacking. From a patient perspective quality is not a simple concept but is best understood in terms of five dimensions: technical outcomes; decision-making efficiency; amenities and convenience; information and emotional support; and overall patient satisfaction. Failure to consider quality from this five-pronged perspective results in a focus on medical outcomes, without considering the processes central to quality from the patient's perspective and vital to achieving good outcomes. In this paper, we argue for applying the concept of fair process in clinical settings. Fair process involves using a collaborative approach to exploring diagnostic issues and treatments with patients, explaining the rationale for decisions, setting expectations about roles and responsibilities, and implementing a core plan and ongoing evaluation. Fair process opens the door to bringing patient expertise into the clinical setting and the work of developing health care goals and strategies. This paper provides a step by step illustration of an innovative visual approach, called photovoice or photo-elicitation, to achieve fair process in clinical work with acquired brain injury survivors and others living with chronic health conditions. Applying this visual tool and methodology in the clinical setting will enhance patient-provider communication; engage patients as partners in identifying challenges, strengths, goals, and strategies; and support evaluation of progress over time. Asking patients to bring visuals of their lives into the clinical interaction can help to illuminate gaps in clinical knowledge, forge better therapeutic relationships with patients living with chronic conditions such as brain injury, and identify patient-centered goals and possibilities for healing. The process illustrated here can be used by clinicians, (primary care physicians, rehabilitation therapists, neurologists, neuropsychologists, psychologists, and others) working with people living with chronic conditions such as acquired brain injury, mental illness, physical disabilities, HIV/AIDS, substance abuse, or post-traumatic stress, and by leaders of support groups for the types of patients described above and their family members or caregivers.
Medicine, Issue 48, person-centered care, participatory visual methods, photovoice, photo-elicitation, narrative medicine, acquired brain injury, disability, rehabilitation, palliative care
Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
Institutions: Pall Life Sciences .
We describe the complete process of AcroPrep Advance Filter Plates for 96 plasmid preparations, starting from prokaryotic culture and ending with high purity DNA. Based on multi-well filtration for bacterial lysate clearance and DNA purification, this method creates a streamlined process for plasmid preparation. Filter plates containing silica-based media can easily be processed by vacuum filtration or centrifuge to yield appreciable quantities of plasmid DNA. Quantitative analyses determine the purified plasmid DNA is consistently of high quality with average OD260/280
ratios of 1.97. Overall, plasmid yields offer more pure DNA for downstream applications, such as sequencing and cloning. This streamlined method of using AcroPrep Advance Filter Plates allows for manual, semi-automated or fully-automated processing.
Molecular Biology, Issue 47, Plasmid purification, High-throughput, miniprep, filter plates
Principles of Rodent Surgery for the New Surgeon
Institutions: Research Models and Services.
For both scientific and animal welfare reasons, training in basic surgical concepts and techniques should be undertaken before ever seeking to perform surgery on a rodent. Students, post-doctoral scholars, and others interested in performing surgery on rodents as part of a research protocol may not have had formal surgical training as part of their required coursework. Surgery itself is a technical skill, and one that will improve with practice. The principles of aseptic technique, however, often remain unexplained or untaught. For most new surgeons, this vital information is presented in piecemeal fashion or learned on the job, neither of which is ideal. It may also make learning how to perform a particular surgery difficult, as the new surgeon is learning both a surgical technique and the principles of asepsis at the same time. This article summarizes and makes recommendations for basic surgical skills and techniques necessary for successful rodent surgery. This article is designed to supplement hands-on training by the user's institution.
Basic Protocols, Issue 47, Surgery, aseptic technique, rodent, training, rat, mouse,
Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching
Institutions: University of Rochester, University of Rochester.
Multi-fluorescence recovery after photobleaching is a microscopy technique used to measure the diffusion coefficient (or analogous transport parameters) of macromolecules, and can be applied to both in vitro
and in vivo
biological systems. Multi-fluorescence recovery after photobleaching is performed by photobleaching a region of interest within a fluorescent sample using an intense laser flash, then attenuating the beam and monitoring the fluorescence as still-fluorescent molecules from outside the region of interest diffuse in to replace the photobleached molecules. We will begin our demonstration by aligning the laser beam through the Pockels Cell (laser modulator) and along the optical path through the laser scan box and objective lens to the sample. For simplicity, we will use a sample of aqueous fluorescent dye. We will then determine the proper experimental parameters for our sample including, monitor and bleaching powers, bleach duration, bin widths (for photon counting), and fluorescence recovery time. Next, we will describe the procedure for taking recovery curves, a process that can be largely automated via LabVIEW (National Instruments, Austin, TX) for enhanced throughput. Finally, the diffusion coefficient is determined by fitting the recovery data to the appropriate mathematical model using a least-squares fitting algorithm, readily programmable using software such as MATLAB (The Mathworks, Natick, MA).
Cellular Biology, Issue 36, Diffusion, fluorescence recovery after photobleaching, MP-FRAP, FPR, multi-photon

domain: medical


text:
Fatal Accident in Martin County Involving Mac Trailer
A run-in with a large commercial truck yesterday resulted in the death of the driver of a pickup truck in Martin County, Texas. According to a representative with the Texas Department of Public Safety, the 50-year-old pickup driver from San Antonio was pronounced dead at the scene. His passenger, a 22-year-old from Atascosa, was taken to Midland Memorial Hospital.
The commercial truck was a MAC trailer traveling west on Tom Craddick Highway. The driver of that truck was apparently not injured in the crash.
It's not clear, based on the Odessa American report, what led to the truck accident. Both vehicles appeared to have been traveling west on the same road, not far from State Highway 349, when the driver of the pickup hit the left side of the MAC trailer. No other details were provided.
It's far too early, based on the facts at hand, to determine whether or not there was negligence involved in this accident. But as it stands, this is yet one more fatal accident on Texas roadways involving large commercial trucks.


text:
by Dr. Ooi Hooi Leng
Consultant Paediatrician and Paediatric Endocrinologist
MBBS (Malaya), MRCPCH (UK), AM (Mal), Fellowship in Paediatric Endocrinology and Diabetes (Malaysia and Australia)
KPJ Klang Specialist Hospital
How do I know if my child is obese?
Calculating the body mass index (BMI) is one way to determine if a child is underweight, normal, overweight or obese. It compares a child’s body weight in relation to his or her height using the formula below:
BMI = Weight (kg)
BMI increases as a child grows. Therefore, the BMI calculation for children or adolescents must be compared against age and gender specific charts. The current BMI charts have been developed by the United States Centre for Disease Control. They are useful to assess if children aged over two are overweight or obese.
BMI-for-age ≥ 85th percentile suggests that a child is overweight whereas a BMI-for-age ≥ 95th percentile indicates obesity.
The BMI calculation should be used as a guide only. Your paediatrician can help to assess whether your child’s weight is a reason for concern.
How common is childhood obesity?
Changing lifestyle and dietary patterns have contributed to increasing obesity rates in children. Childhood obesity is a major public health problem in many developed and developing countries. In theUnited States, approximately 24 per cent of children and adolescents are overweight and another 18 per cent are obese. InMalaysia, data from various researches have estimated that as many as 15 per cent of toddlers and 30 per cent of primary school children are overweight or obese.
Will children outgrow obesity?
Not really. Obese children tend to become obese adults, especially if they have the following features:
a) Become obese after the age of three. 55 per cent of obese children between six to nine years old become obese adults and the risk increases to 75 per cent for those between 10 to14 years old.
b) The more severe the degree of obesity, the higher the likelihood of persistence.
c) Obesity in one or both parents.
What are the complications of obesity in children?
Obesity can result in serious health problems in childhood and later life. It predisposes them to:
a) Insulin resistance and type 2 diabetes especially if there are family members with diabetes.
b) High blood pressure and elevated bad cholesterol. This will cause narrowing or blockage of arteries by the cholesterol plaque, thus predisposing them to early heart attack or stroke.
c) Sleep apnea, which is breathing difficulty during sleep. It also causes snoring, frequent wakening and poor sleep, resulting in tiredness and contributing to poor concentration during the day.
d) Liver problems including fatty liver.
e) Increased body weight results in excess load to the bones and joints causing early wear and tear especially to the lower limbs and feet, resulting in bone pain and deformities.
f) Most obese children will experience psychological problems including low self- esteem, teasing by friends in school, social isolation, anxiety or depression.
What are the strategies for combating childhood obesity?
a) Preventing our child from becoming obese is far easier and less painful than asking them to lose the excess weight. In growing children, it is important to at least maintain current weight so that the BMI decreases as the child grows taller.
b) Promote breastfeeding. Exclusive breastfeeding from birth until six months and continued breastfeeding up to two years should be encouraged. Various studies have shown a weak but consistent protective effect of breastfeeding on preventing the development of obesity.
c) Adopt a healthy lifestyle for the whole family. Be a good role model by keeping your own BMI within the normal range. Implement a healthy diet and active lifestyle for the whole family and not just for the children.
d) Avoid crash diets, appetite suppressants or humiliating or teasing an overweight child as it does not help them in losing weight.
How can I ensure a healthy diet for my child?
e) Avoid fast food, deep fried food, junk food, sugary food and food cooked with coconut milk. Use less oil, and cook more grilled or steamed dishes. Eat more vegetables and fruits. Limit takeaway and fast food.
f) Make water the main drink. Avoid sugary soft drinks, processed fruit juices or cordial. Have low fat or skim milk.
g) Eat breakfast to prevent them from consuming too much snacks or large meals during lunch.
h) Provide healthy snacks that are low in calories. Fruits and vegetables make excellent choices.
i) Offer smaller portions. Serving 10 or 20 per cent less over time can make a significant difference.
j) Have meals as a family at least once a day. Make mealtime a happy time.
k) Avoid rushing to finish meals. Eating too quickly does not allow time for digestion or feeling a sense of fullness.
l) Avoid eating while watching TV.
m) Encourage babysitters/maids and grandparents not to give children unhealthy foods.
n) Educate your child to make healthier choices at the school canteen.
What is an active lifestyle?
a) Be active for at least one hour a day. Encourage lots of opportunities for active play for pre-school children and toddlers. For kids, exercise is about having fun. The activity can also be incorporated into their daily routines and carried out in small amounts throughout the day, e.g., using the staircase instead of the lift, helping with the household chores or playing with friends in the evening. Be active as a family and plan family outings together.
b) Limit total screen time to two hours a day. This includes the TV, computer, iPad and playing electronic games.


text:
You may remember hearing about the P2P puesdo-currency Bitcoin: a virtual monetary system that allows users to pay anonymously, bypass bank regulations, and avoid transfer and withdrawal fees. Last year, Bitcoin experienced quite a crash and security breach that made the currency nearly worthless — dropping from the exchange rate of $17 to merely a few pennies. Bitcoin has surprisingly got itself back together in the past months, and now a New York City-based startup wants to normalize its use again by introducing a Bitcoin debit card system.
Known as BitInstant, this Bitcoin debit card would act like your average prepaid card that you can use to make every day purchases, withdraw money, or transfer amounts to friends and merchants. The card hold a maximum of $1,000 in Bitcoin credit (BTC) limit, and would contain QR codes in the front that are scannable by a BitInstant app to link users directly to their accounts.
“You’re at dinner with a friend? Forget PayPal, just scan his or your card with your Bitcoin app,” BitInstant CEO Charlie Shrem explains to Ars Technica. This process bypasses transfer fees if friends do not bank with the same company though users are allowed to keep each money transfer as BTU or convert them into US dollars, in which case a conversion fee would be charged.
So why would anyone use a Bitcoin debit card in place of actual prepaid cards in the first place? For one, BitInstant would attract those who’ve actually had successes at mining Bitcoin. Since the e-currency is in its own entire realm, advanced Bitcoiners can run complex softwares to generate BTC and BitInstant would allow these users to spend their hard-earned Bitcoins in real life, anywhere they want. The transfer would also be as instant as handing someone cash, meaning transactions can take place between any two users in the world without waiting for third-party middlemen to process the transfer.
Still, the entire concept of BitInstant cards seem ironic, to say the least. The point of Bitcoin is to provide anonymity and leave banks out of the money transfer equation, yet introducing a bank-issued Bitcoin card would bring both issues back to the forefront. We can’t imagine that a bank would issue a BitInstant card without so much as providing a trackable feature on the card, ruining the whole anonymous transaction feature Bitcoin had going for it. Even if BitInstant and the banks that issued the cards cannot publicly release transfer history made on the card, we’re pretty sure one can get a hold of that information if a user can acquire a police warrant to make banks give them that data.
But hey, if the whole futuristic e-currency idea seems fancy to you, by all means, preorder your Bitcoin debit card with BitInstant and its public Google Doc signup sheet.
(Photo mockup of Bitcoin debit card by Ars Technica.)

domain: finance


Phasing of the ELYSSAR Development Program
The development program is
expected to be executed in four major phases,
4, over a fourteen year period, to include the
- 10,120 Residential
- 1,595 Shops.
- 873 Workshops.
- 7,040 of Major roads.
of Housing Infrastructure
of Infrastructure upgrade.
of New Infrastructure.
Table of Project Excecution for all Phases


text:
Do you need to have an vast elements onto your Trivia Live Quiz Game Show & Play Bingo Game to Win Cash gaming specify free? It is not recommended to wait right now! Experiment with our own unique Trivia Live Quiz Game Show & Play Bingo Game to Win Cash Cheat Trick. It will be the best with this Live Quiz Game Show & Play Bingo Game to Win Cash Trivia Hack, you will gettremendous advantage so simple and quick! Live Quiz Game Show & Play Bingo Game to Win Cash Trivia Cheat tool operates straight from the browser, without in the process of being known. Without using thedownload, setup anything on your appliance, you can forget aboutpotential risk of virus. Usually use our Live Quiz Game Show & Play Bingo Game to Win Cash Hack Tool and you’ll be very satisfied.
This powerful Live Quiz Game Show & Play Bingo Game to Win Cash Cheat that we both should always publish for your requirements will let youon other ways. Taking advantage ofthis cheat, you’ll be able to recieve a large amount of Live Quiz Game Show & Play Bingo Game to Win Cash online gaming assets gratis. You will never need to run out Live Quiz Game Show & Play Bingo Game to Win Cash game assets to be usedin internet gaming. This factor indicates that you possess an factor that is a benifit to several other online players because you have the ability tocan just actually purchase resources whenever youwant. Having this, you can aquiremore Live Quiz Game Show & Play Bingo Game to Win Cash game assets to obtain.
Live Quiz Game Show & Play Bingo Game to Win Cash Hack would be the most fascinating over the internet technique for cell phones announced in our dayby our community! Enter our Internet based Live Quiz Game Show & Play Bingo Game to Win Cash Resources generator Unrestrained, 100% free and initiate to generate A great number of assets in you your game account. Online generator, quintessentially, is on Android, microsoft windows and iOS Phone. We agreed to build up Live Quiz Game Show & Play Bingo Game to Win Cash Hack tool after trying the gameplay instantly. This Trivia game is without a doubt gorgeous! Our software engineers they started out their effort to your cell phones to assist understand and follow wishes considering the individual users. It most definetleyis an tremendous trend, we could definitelyself convinced where the entire Live Quiz Game Show & Play Bingo Game to Win Cash game cheat there is no doubt that you willland to your flavor. ExamineLive Quiz Game Show & Play Bingo Game to Win Cash cheat and revel in the gameplay at the best level now! Our Live Quiz Game Show & Play Bingo Game to Win Cash online hack tool operates besides Android and iOSdevices. You don”t really need tohave root or jailbreak to utiliseour Trivia Live Quiz Game Show & Play Bingo Game to Win Cash hack.
BaaziNow Live Gaming App – Play the live quiz and bingo game show, win and get instant cash in PayTM or Mobikwik wallets
BaaziNow is a Live Free Game Show by Times Internet Limited where you can win instant cash in your PayTM or Mobikwik wallets. BaaziNow App features BrainBaazi – a live quiz game show and BingoBaazi – a live bingo games show where you can play trivia quiz and online bingo game with millions of other users.
Download the BaaziNow app now, play BrainBaazi – live quiz and test your trivia quiz skills at 8:30 PM for Rs. 1,00,000 prize every day and afternoon show at 1.00 PM for Rs. 50,000 prize Monday to Friday or play live bingo game at 4.30 PM daily for Rs. 50,000 prize. Refer BaaziNow online trivia quiz app and bingo game app to your family, friends & colleagues and get an extra life.
BingoBaazi – Live Bingo Game Show
Participate in BingoBaazi –live bingo game daily at 4.30 PM. Play online bingo game with millions of users and win cash. The anchor will call out numbers live, for which players will get 6 seconds to confirm. Enjoy bingo live game app to win loads of prizes! Don’t miss out! Download now to enjoy the bingo game!
How to Play Bingo Live?
* Download the BaaziNow online game app from PlayStore
* Sign up using your mobile number & an OTP
* Go to BingoBaazi to play live bingo game
* Join the game every day at 4.30 PM to get a ticket, each having 3 rows & 15 numbers
* Players get 4 chances to win top, middle, bottom rows and the full house
* Anchor will announce a number, players will get only 6 seconds to confirm each number
* If all the numbers in a row match the announced numbers, you will win the row prize
* After the claim, our system will verify the eligibility and prize money will be credited in your E-wallet
* If all the numbers of your ticket match the announced numbers before our 36th call, you will win the grand prize for Full House
* Claimed prizes will be available at the top of the screen
* In case your claim is incorrect or you mistakenly touch a wrong number, your claim fails which is called a bogus call. We allow one bogus call per bingo game. So don’t worry, you can still play the game
BrainBaazi – Live Trivia & Quiz Game Show
BrainBaazi is one of the most innovative online trivia quiz games in India where you get a chance to compete in a live quiz with millions of other players as the host takes you through the show. The host will ask you 11 questions, which you have to answer correctly to win the cash prize.
How to Play Live Quiz?
* Go to BrainBaazi to play the quiz online
* The host will brief you about the rules of the game
* The host will ask 11 (eleven) questions, each having three options. Only one of these three options is correct
* Players will get only 10 seconds to answer each question
* If your answer is incorrect or you run out of time, then use your extra Life to be in the game
* While rules may change in the future, but at present, you can only use one Life in one game
* Answer the Q9 – Kal-Nayak Sawaal correctly without using your life and get an extra life at the end of the quiz game. Both eliminated & non-eliminated users can answer this question
* Answer the 10th question correctly without using your life & you will be credited with your share of Rs. 30,000
* Every day 10 minutes before the quiz starts, the host will share cheat code. Remember this hint and use it during the quiz to increase your chances of winning
The app needs following permissions to function properly:
Location: To optimise your streaming experience basis your geo
SMS: For mobile number verification
Mobile Number: To link your Paytm/Mobikwik wallet to transfer your winning amount. We also use this to send you periodic show reminders
Follow us on Twitter & Facebook
Introducing BingoBaazi, India’s first and only live bingo game. Play Bingo and win cash!
The mother app is being rechristened as BaaziNow and both BrainBaazi and BingoBaazi will be housed within this app. BrainBaazi is all about brains and trivia knowledge. Our new game BingoBaazi is all about numbers and your luck.
Tags: Live Quiz Game Show & Play Bingo Game to Win Cash 2.0.20 APK, Live Quiz Game Show & Play Bingo Game to Win Cash APK MOD, Live Quiz Game Show & Play Bingo Game to Win Cash Cheat, Live Quiz Game Show & Play Bingo Game to Win Cash Hack, Live Quiz Game Show & Play Bingo Game to Win Cash Hack Android, Live Quiz Game Show & Play Bingo Game to Win Cash Hack iosgods, Live Quiz Game Show & Play Bingo Game to Win Cash Hack No Survey, Live Quiz Game Show & Play Bingo Game to Win Cash resources generator
Developer Acid Ape Studios
Uploaded February 13, 2019 20:03
Filesize 27.9 MB
Uploaded January 11, 2018 14:48
Filesize 19.8 MB
Developer Parako Games
Uploaded May 23, 2018 02:14
Filesize 36.5 MB
Developer Team Bad Buoys
Uploaded July 25, 2018 13:05
Uploaded August 06, 2018 15:35
Filesize 52.4 MB
Developer Galaxy Action Games
Uploaded January 11, 2019 10:01
Filesize 55.5 MB
Uploaded February 25, 2018 14:14
Filesize 95.3 MB
Uploaded July 14, 2018 01:31
Filesize 3.1 MB
Developer Son of styx
Uploaded May 15, 2018 02:14
Filesize 32.6 MB
Copyright© 2017 Game Quotes


text:
photo credits: Wikimedia Commons
country of citizenship: United Kingdom of Great Britain and Ireland
native language: English
languages spoken, written or signed: English
educated at: Bedford School
occupation: writer, historian, novelist, playwright, journalist
Hector Hugh Munro (18 December 1870 – 14 November 1916), better known by the pen name Saki and also frequently as H. H. Munro, was a British writer whose witty, mischievous and sometimes macabre stories satirize Edwardian society and culture. He is considered by English teachers and scholars as a master of the short story, and often compared to O. Henry and Dorothy Parker. Influenced by Oscar Wilde, Lewis Carroll and Rudyard Kipling, he himself influenced A. A. Milne, Noël Coward and P. G. Wodehouse.Besides his short stories (which were first published in newspapers, as was customary at the time, and then collected into several volumes), he wrote a full-length play, The Watched Pot, in collaboration with Charles Maude; two one-act plays; a historical study, The Rise of the Russian Empire (the only book published under his own name); a short novel, The Unbearable Bassington; the episodic The Westminster Alice (a parliamentary parody of Alice in Wonderland); and When William Came, subtitled A Story of London Under the Hohenzollerns, a fantasy about a future German invasion and occupation of Britain.
Read more or edit on Wikipedia

domain: artistic


If you are interested in our company and our products, you can click to consult or leave a message, we will provide you with value-for-money equipment and thoughtful service.
Send Email:[email protected]
Chat Online Information
Cement Mill is one of the important cement making equipment and key equipment for grinding crushed materials, and plays a vital role in the cement manufacturing process.
Ball mill is the key machine to grind the crushed materials. Ball mill machine is widely used to process cement, silicate product, building material, refractory material, fertilizer, ceramics and glass, etc.
Charcoal briquette machine is also known as charcoal briquetting machine, charcoal ball press machine, or charcoal pressure ball machine.
Single cylinder rotary cooler is suitable for cooling clinker out from rotary kiln. Rotary drum cooler can rapidly cool clinker out from rotary kiln from 1000-1300 ℃ to 200 ℃).
VSI sand maker, also called VSI crusher, is the major machine for sand making plant. During the crushing process of sand making machine, raw materials hit each other and not hit any metal parts.
The high pressure briquetting machine or the high pressure ball press machine is used to press many kinds of materials such as gypsum, coal powder, mineral powder, scale cinder, slag, fine iron powder and aluminum ash etc.
Rotary kiln belongs to cement and building materials making equipment, is necessary equipment for cement plant to produce high grade cement.
Rotary dryer is one kind of drying equipment, generally used to remove water containing in materials. Most of rotary drum dryer consists of a rotating drum driven by belt, and the hot air around the drum is used to evaporate water.
2 days ago018332The companys feasibility study, released last November, predicted annual EBITDA of 16.4 million based on an Australian dollar iron ore price of 111.43 a tonne -Read More
Jul 14, 2020018332The iron ore boom powering the profits of the worlds biggest mining companies is showing no sign of fading thanks to Chinese steel demand. ... Indonesias Richest. Koreas Richest.Read More
Australia, through the CEO of Fortescue Metals Group FMG Andrew Forrest, is eyeing the development of Indonesias geothermal environmentally friendly energy of 25 GW. FMG is an Australian iron and nickel mining company that is among the top 10 in the world. To open the plan, Andrew Forrest met President Joko Widodo September 3, 2020.Read More
We are a new mining company in Indonesia. Our iron ore mining concession area is one of the largest in Indonesia at this time. We can supply you with iron ore lump and iron sand. We believe we can satisfy our buyers and our investors.Read More
Iron Ore Screen In Indonesia. Jun 01 2018018332The work analyzes the performance of the main type of screen used in classification of green iron ore pellets the roller screen through simulations using the discrete element method Recognition of the sticky nature of the material led to the choice of the HertzMindlin model with JKR cohesion to describe the contactsRead More
The Liberia Mining Company was the first of four iron ore companies which produced and shipped such large quantities of iron ore that in the 1960s and 1970s Liberia became Africas largest iron ore exporter and even the third on the world list of iron ore exporters.get priceRead More
Detailed investigation established the reserves to be about 200 million tonnes with an approximate iron Fe grade of 36. Decree No. 60 of 19th September, 1979 dissolved the NSDA and established six companies, one of which was Associated Ores Mining Company AOMC. AOMC was later renamed National Iron Ore Mining Company NIOMCO in February 1987.Read More
We buythe followings1. Mineral Ores 1. Copper ore 10-20 up, 2K-100K mt per month 2. Gold Ore 10gmt 5k-100k 3. Lead Ore 45 2k-5K 4.Read More
List of Iron Ore Companies in Indonesia. Gading Agrokom Industry is a company that have core business in Mining and Trading of Mineral, specificly iron ore coming from South of Aceh, Indonesia.Read More
iron ore production costs in indonesia and iron mining. l Mine Operating Costs new in this years report Iron ore producers have been able to reduce mining costs substantially over the past four years, and the mining industry as a whole now spends US22dmt less than it did in 2013 due to tightened capital controls, renegotiated contracts and the exit of high- .Read More
Oct 22, 2020018332The Lokayukta report had said that 3.31 metric tonne of illicit iron ore was exported from 2006-2010. It had noted that under the garb of issuing temporary transportation permits to lift and transport iron ore, large scale illegal mining activity was being allowed to be carried out for a certain period, even in the forest areas.Read More
Sizisa Mining Started as a trading company it then went on to acquire its own resources keeping market share intact. has now the following mines1. Chrome ore mine in Zeerust North West South AfricaReserves 40 million tonsMine monthly ...Read More
Jun 03, 2019018332Steel companies have urged the government not to extend the merchant iron ore mining leases that are expiring next March, as it could lead to aRead More
The iron ore from the project would be supplied to the Corus facilities in the UK and the Netherlands. Tata Steel also has a greenfield steel project in Vietnam, which includes a 30-per cent stake in Thach Khe Iron Ore joint stock company. The joint stock company would undertake mining in the Thach Khe iron ore mine. The move to step up raw ...Read More
Jul 18, 2019018332Indonesia stopped exports of raw ore such as nickel, bauxite and concentrates of other minerals from 2014 to 2016, and the revenues of mining companies such asRead More
Top International Mining Companies. Links to suppliers of coal, coke, ores General Mining amp Minerals. Anglo American Copper, nickel, iron ore, metallurgical and thermal coal Glencore Metals and minerals, energy products, agricultural Products Itochu Trading in energy, metals, minerals Japan Ivanhoe Mines Interests incl SouthGobi Resources amp Ivanhoe AustraliaRead More
Jan 05, 2018018332NDMC is Indian mining company owned by Indian Government founded in 1958. It is Indias biggest iron ore producer and one of the biggest iron ore producers in the world as well. Apart from iron ore business, they also extract copper, limestone, dolomite, bentonite, diamonds, etc., which are being extracted from four mines in India. 4.Read More
Twelve companies have been granted permits to begin mining iron ore in the Sheini reserve of the ZabzuguTatale District of the Northern Region. According to the Minerals Commission, the 12 companies were given the due diligence permits to allow each of them to search for the mineral within the area by geo-chemical and photo-geological surveys ...Read More
Nov 23, 2020018332Gold mining companies are active on all continents on earth. This wide geographical distribution in production means that interruptions in any single region due to local political or social matters are unlikely to have a large impact on the global gold supply.Read More
Asia-Pacific Vietnam, China, Malaysia, Japan, Philippines, Korea, Thailand, India, Indonesia, and Australia Global Iron Ore Industry Market Competitive Rivalry. The chapter on company profiles studies the various companies operating in the global Iron Ore Industry market.Read More
Oct 23, 2020018332Our Iron Valley site, located within the Pilbara, is currently our largest iron ore producing mine, outputting approximately 8 Million tonnes of iron ore per annum. Product from this mine is transported to Utah Point for export to our global markets. In 2018, we acquired the Kumina Iron Ore Project from BCI Minerals Limited.Read More
The Major Australian Iron Ore Mining Companies The two biggest companies that mine iron ore in Australia are Rio Tinto and BHP Billiton Rio Tinto operate the giant Hamersley Iron Ore project at Mount Tom Price in Western Australia, as well as the Western Turner Syncline mine, the HiYandi, Marandoo, Eastern Range, Channa, Paraburdoo and ...Read More
Home gt Client Markets gt Mining amp Metals gt Mining gt Iron Ore Iron Ore Fluor Mining Expertise For more than 40 years, Fluor has been executing iron ore projects globally, providing a full range of program management, study and engineering, procurement, fabrication, construction EPFC and maintenance services.Read More
The mining company had maintained its mining targets to get to 290 million tonnes per annum. Price volatility in the Australian iron ore mining industry is expected and there will continue to be highs and lows on a regular basis. However, the big mining companies in Australia seem confident of a steady iron ore mining industry in Australia.Read More
About 80 of Chinas iron ore imports come from the four largest mining companies -- Brazils Vale S.A. and Australia-based Rio Tinto, BHP Group Ltd. and Fortescue Metals Group.Read More
Jan 18, 2019018332quotGlobal Iron Ore Mining to 2022quot provides a comprehensive coverage on global iron ore industry. It provides historical and forecast data on iron ore production by country, grade, iron oreRead More