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As much as I want to like this game, I just can't.
I grew up with Megaman, so he's my most favorite video game character. Megaman, to me, is like his own genre of games, unique and different from other games, yet it can still be related to other games.
My first and foremost problem is that this game is not fun. Usually when a good game is made, you want to explore the levels and see what it takes to build something like them. This game, however, is not like that. I commend you for using your own graphics, but its not seamless and it lacks fluency and well thought out stage design. I constantly find myself trying to get through the stage as fast as I can, and not worry about anything until I get to the boss. Honestly, I can go into the ocean stage and hold down dash button and jump around until I reach the boss. When someone does this, then the map design has failed and is not worth taking a second look at the stage.
My second problem is the boss difficulty on NORMAL mode. You, AT NO POINT, never give the boss a one-hit-kill attack. At this point, it seems to me that the game discourages exploration, and punishes one for not being able to dodge an attack, even though you so generously gave us 3 health bars which means nothing if you can't dodge the boss' ultimate attack; it tears threw it like nothing.
The third flaw I found was also in the ocean stage. The part where you have to climb down the ladder to get to the next screen is most aggravating. If you jump off to the side, and I mean if one pixel is out of place, you are GOING TO DIE. I've never seen a game that requires that you gingerly make your way down a ladder as opposed to jumping off the side to get to the next screen when they both obviously take you to that same place.
Fourth, the spikes. I can understand when you make the spikes chew threw your life as if it were nothing, thats the point (however, in most cases, it means a one-kit-kill). But when you litter a stage with these things, its not uncommon that I want to get trough the stage as fast as possible without having to run into these things. They get annoying, and fast. Granted, I do like the fact that I get second chance if I get out fast enough. Still, it's a common flaw in stage design for people to over step where spikes should be placed.
What do I like about this game? Hard to say. Good attempt, but hopefully more games that wish to do something like this won't follow the same path.
(Since this game was made 4 years ago, I'll give it the benefit of the doubt and assume that the owner has made something far superior to this.)
- Stages are meant as filler and eye candy to me, I put them above menu's on my priority list usually. In the younger years of game making here I still hadn't quite got the balance down of obstacles, enemy placement and length (still working on it too). On the lower difficulty settings you probably can dash through a good few stages without dying, probably not so much on the higher ones. The other thing being is filesize. More stage sections = more file size. More fancy graphics to pretty up stage sections = more file size. More file size = more bandwidth used and in Newgrounds case you can hit the file size cap. So it becomes a juggling act on where priorities lie.
- The "One Hit Kill" thing is a false statement on two levels. Plenty of games do this, heck games like Contra give you only 1 hit period before you lose a life. The second reason this is false is because outisde of bottomless pits, no attack if the game kills you in one hit. Some attacks do "Damage Over Time" which can potentially kill you, but that's only because you sat in it to long. It's like they say in MMO's, "Don't Stand in the Fire". Don't blame me if you don't dodge properly.
- The Ocean Stage ladder thing is due to me not being a good enough programmer at that point to be honest. Though parts of your statement are also flatly wrong, mainly that they both obviously take you to the same place. The stage section you are coming from has a large bottom section, let's say 800 pixels. Now part of that obviously needs to be for the bottomless pits for the first part of the stage, but you'd technically want part of it to be available to proceed to the next stage section. The next stage section has a landing spot built into it of like 100 pixels lets say. Obviously directly translating a character's X position to the next stage when there's up to a 700 pixel difference in location is not going to work without drastically increasing the landing spot in the next stage section to accommodate the player's original X position. Now in later incarnations I've learned to do things like the invisible walls or just auto assigning the players X/Y position to force them to appear in the next stage section where they should regardless of where they were exiting the last stage section. Quick fix for me: make the player stick to the ladder for that part though it's not ideal (limits of skill are limits of skill after all).
- The spike comment is pointless and flat out false. I'm forgiving if you touch'm for a second as opposed to instant death, but if you want to rush through the stages and die while doing it that's your fault. It's not like the spikes get up and chase you down, they're just sitting there mind their own business. True there are some parts that have more spikes than others, but that's part of stage design and plenty of other games out there do it too. It's common flaw in players to blame the programmer for their own inability to adapt to the situation at hand because they don't want to stop doing something that's inappropriate at the time or not paying attention (ie: recklessly holding the dash key ignoring the row of spikes coming up).
Good overall review. Roll Next and Mega Man Next Remix are floating around out there with most of the technical parts touched up better, but if you still like to go charging gung-ho into spikes and standing in the fire they'll probably rank about the same for ya.
Great game but------------> last post is a hoax
Don't type that into your browser!! It's a virus!!
I looked at a couple of submissions, then I went to play the game. I really knew this was going to be a scary ass game, kinda like a horror flick. I went into the game, looked around and found my way inside various rooms. I played for a little while until I started to see faces emerge from the shadows... I was like fuck this, I'm leaving. This game freaked me out, and I'm scared to play again... But I guess thats what makes games good.
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domain: game


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Ames 2825500 2-Prong Weeder Hoe, 9 in W x 3-1/2 in L Tempered Steel Blade, 54 in North American Ash Handle
PRODUCT OVERVIEW: Designed for close use around existing plants. 10" cushion grip for comfort and control. Dual head design for weeding and cultivating. The flat "hoe" side is used for chopping, weeding and clearing light garden growth. The pointed "prong" side is used for cultivating the soil. North American hardwood handle for strength and durability. Dimensions: 54.25"H x 9"W x 3.5"D.

domain: design


text:
BBQ Pulled Pork Sandwiches - Sloooow Cooked in Your Crock Pot
photo by Pellerin
- Ready In:
- 10hrs 20mins
- 4 -6 lbs pork shoulder (aka "Boston Butt", NOT loin. Have your butcher tie it for you, you want the STRINGY SHOULDER!)
- 1⁄4 cup Emeril's Original Essence (or your other favorite BBQ rub mixture.)
- 2 tablespoons vegetable oil
- 1 (15 ounce) can diced tomatoes (I like fire-roasted)
- 1⁄2 cup ketchup (Heinz)
- 2 tablespoons tomato paste
- 1 cup dark brown sugar (packed)
- 1 cup red wine vinegar
- 1⁄2 cup prepared yellow mustard
- 1⁄2 cup Worcestershire sauce
- 1 1⁄2 tablespoons liquid smoke
- 2 teaspoons salt
- 1 teaspoon ground black pepper
- 1 teaspoon garlic granules
- 1⁄2 teaspoon onion powder
- 1⁄2 cup cornstarch
- 1 (14 ounce) can low sodium beef broth
- Spread seasoning run liberally over all meat. Place rubbed roast into a large Zip-Lock plastic bag. Chill overnight, or at least several hours. Recommend you make sauce at the same time, so it's ready to go the next day.
- Preheat regular oven to 225, or crock pot to medium/low.
- In a large, heavy Dutch oven, heat oil to medium. Brown the seasoned roast until all sides are caramelized. Remove excess fat. Combine all SAUCE ingreadients, and pour over meat. Cover and cook for 8 - 10 hours.
- Remove meat from pan. Place on large plat and cover with foil until cool enough to handle. Whilie meat is cooling, combine the cornstarch and beef broth with a fork, to remove lumps. Add this thickening agent to the sauce in the pan, to creat the BBQ sauce. Bring to a gentle boil, without the lid, and simmer until thickened. Taste for seasonings.
- When meat is cool, shred meat by pulling with a fork. Add meat to sauce, and discared any fat and butcher's twine. Simmer approximately 30 minutes to let flavors meld. Serve as a sandwich on warm potato hamburger buns with coleslaw &/or potato salad. Many people like the slaw ON the sandwich. Freezes well.
Questions & Replies
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I had a craving for pulled pork today and decided to try this recipe. I concocted my own rub using sweet paprika, chile flakes, garlic and onion powder, salt, pepper, and Goya Adobo with Cumin. I used a bone-in shoulder and trimmed off as much skin as I could. I forgot to brown it before putting it in the crock-pot, but I don't think it made too much of a difference. I only had about 1/2 T of liquid smoke, it definitely needed the full 1 1/2 T that the recipe calls for. The sauce is delicious, not too tangy, not too sweet. I actually omitted the part with the beef broth and cornstarch, I thought the sauce was thick enough without it. If anyone has any suggestions for removing the fat that's left in the sauce I would appreciate it, it always becomes a time-comsuming process for me. Great recipe, I am bringing some to my mom's tomorrow for her to try.
We used this recipe for a community function. We had 20 lb of cooked pork roast and decided to serve it as pulled pork. We made the sauce and adjusted the amount for the amount of meat we had and added 5 cups of sauted thinly sliced onions and 5 cups of chopped celery. We mixed it with the pork and refrigerated it overnight. The next day we put it in the oven at 325 and cooked it for 4 hours. It was wonderful served on large kaiser buns!!
an easy way to get rid of all of the accumulated fat. after the pork is finished cooking, drain the juices and put them in a container in the fridge (or freezer if you're in a hurry). the fat will float to the top of the container and you'll be able to just scrape it off with a spoon. i usually make my pork roasts a day ahead and leave the juices in the fridge over night.

domain: food


I can hardly believe the news but Milk star James Franco is headed to Daytime Soap, General Hospital! Not only is he is going to have a short-term role but TVGuide.com is reporting that is was HIS idea!
“He loves to explore the different types of acting that are available and had never done soaps and was really interested in the amount of work soap actors do—he wanted to try it out,’ says the network rep, noting that Franco contacted GH directly. “Basically, the attitude from the writers and producer is, ‘When you pick up the phone and it’s James Franco saying he wants to work with you, the only answer you have is, ‘Yes!’” Adds the rep with a laugh: “There’s an open invitation to any other A-List movie stars who want to come to General Hospital.”
The star will film his scenes on three different days, October 30, November 6 and November 13—and they will air over the course of two months. His first airdate is set for November 20th.
I wonder what role he will play?! This is gonna be fun to see!!


It seems hardly a year or two back that our former title Fleet Van was handing over the trophy for van of the year to the new Fiat Doblo Cargo. But it was, in fact, back in 2010 and already the Doblo is being treated to a mid-life facelift that looks set to ensure its ongoing success.
When it was first launched, the Doblo offered such high levels of build quality, general driveability and practicality that it immediately catapulted the Italian manufacturer into the premium league for the first time.
Indeed, the Doblo was taken by Vauxhall and rebadged as the Combo – although it appears, surprisingly, that this arrangement will soon cease as Vauxhall joins with Citroën and Peugeot to produce its next new small van. Surprisingly as we really can’t praise the Doblo Cargo highly enough.
The new model features a fresh front-end and various other under-the-metal improvements such as a quieter cab, and better comfort, handling and fuel consumption, which is improved by up to 12%.
There are now five engines to choose from: 1.3-litre, 1.6-litre, 1.6-litre ComfortMatic and 2.0-litre diesels, offering between 90hp and 135hp and a 1.4-litre petrol with 95hp.
Our test model is the Maxi 1.6-litre diesel offering 105bhp and priced £20,668 ex-VAT.
The reworked front-end sharpens up the Doblo’s looks no end, while in the cab there’s a quiet ambience that ensures the occupants can talk to each other without shouting, even at motorway speeds.
We particularly like the Doblo’s driving seat, which is firm and supportive and features an adjustable lumbar support to eliminate back twinges on long journeys. An overhead parcel shelf also comes as standard – some rivals offer this feature as a paid-for option – while there is also a good quality radio/CD player with an auxiliary socket in the middle console for plugging in remote devices.
Sadly, the van’s reversing sensors come as a £185 option. In our view, these should be standard equipment on all commercial vehicles. Manual air-conditioning is optional too, at a hefty £595, putting it out of reach for most fleet drivers. The rival Citroën Berlingo has air-conditioning as standard on Enterprise models. Air-conditioning used to be considered a luxury, but nowadays it is viewed as an essential comfort feature as a cool driver will be more alert and less frazzled in summer weather.
At the cargo end, the Doblo really excels. Maxi it says and maxi it is, offering 4.2 cubic metres of loadspace and more than a tonne of payload, which means this van will swallow an impressive amount of goods. Top marks too, for the standard fitment of a PVC floor covering, which stops bits of cargo sliding around all over the place, and the two side sliding doors which come as standard.
On the road, the Doblo is a delight to drive, with lots of feel through the steering wheel, a slick gearchange and nicely-weighted power steering. The 105bhp powerplant ensures there is plenty of oomph on offer where needed.


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The size and biogeochemical impact of the subseafloor biosphere in oceanic crust remain largely unknown due to sampling limitations. We used reactive transport modeling to estimate the size of the subseafloor methanogen population, volume of crust occupied, fluid residence time, and nature of the subsurface mixing zone for two low-temperature hydrothermal vents at Axial Seamount. Monod CH4 production kinetics based on chemostat H2 availability and batch-culture Arrhenius growth kinetics for the hyperthermophile Methanocaldococcus jannaschii and thermophile Methanothermococcus thermolithotrophicus were used to develop and parameterize a reactive transport model, which was constrained by field measurements of H2, CH4, and metagenome methanogen concentration estimates in 20–40 °C hydrothermal fluids. Model results showed that hyperthermophilic methanogens dominate in systems where a narrow flow path geometry is maintained, while thermophilic methanogens dominate in systems where the flow geometry expands. At Axial Seamount, the residence time of fluid below the surface was 29–33 h. Only 1011 methanogenic cells occupying 1.8–18 m3 of ocean crust per m2 of vent seafloor area were needed to produce the observed CH4 anomalies. We show that variations in local geology at diffuse vents can create fluid flow paths that are stable over space and time, harboring persistent and distinct microbial communities.
The igneous ocean crust contains 2% of the fluid volume of the overlying global ocean  and an estimated 1.5 Pg of microbial carbon . Diverse chemolithotrophic Epsilonbacteraeota living in the global ocean crust at deep-sea hydrothermal vents contain an estimated 1.4–2.7 Gg C and produce 0.045–1.4 Tg of organic C yr−1, suggesting that microbes in the shallow hydrothermal subseafloor are a relatively small standing stock which turns over rapidly . However, quantifying the biogeochemical impact of subseafloor microbes in the anoxic zones of oceanic crust remains a challenge. At Axial Seamount in the northeastern Pacific Ocean (Fig. 1), low-temperature (<50 °C) diffuse hydrothermal fluids steadily emanate from cracks in the basaltic seafloor and contain biogenic CH4 and culturable methanogenic microbes [4,5,6,7,8]. Both molecular and culture-based analyses of these fluids showed that the predominant methanogens present are thermophilic Methanothermococcus and hyperthermophilic Methanocaldococcus species [6, 9,10,11,12]. They are among the most common high-temperature methanogens found globally in hydrothermal vents [13,14,15] and hot subsurface petroleum reservoirs [16,17,18,19].
Communities of methanogens and other microbes at individual vents at Axial Seamount are distinct and persistent over time, demonstrating that the subseafloor habitat consists of stable localized microbial communities that reflect their local environment [12, 20, 21]. However, the inaccessibility of the igneous subsurface makes it difficult to measure the total number of active methanogens that inhabit the subseafloor at each vent site, the environmental factors shaping the composition of these microbial communities, and their biogeochemical impact on the diffuse fluids exiting the seafloor into the overlying ocean. One approach for quantitatively assessing these questions is reactive transport modeling. However, developing models of subsurface microbial growth relies on correctly parameterizing the growth kinetics of the resident microbes. The Methanothermococcus and Methanocaldococcus species dominant at Axial grow primarily by combining 4H2 and CO2 to produce CH4 and 2H2O. Microcosm incubations of diffuse vent fluids from Axial Seamount demonstrated that methanogenesis at 55 °C and 80 °C does not use formate or acetate and was limited by H2 availability rather than anabolic needs such as nitrogen, vitamins, or trace metals . Therefore, these two genera of methanogens are ideal for reactive transport modeling using H2 concentration and temperature as the primary variables.
To predict the minimum threshold of H2 needed for growth, three Methanocaldococcus spp. were grown previously in a gas-purged batch reactor at different H2 concentrations to determine their H2 Monod kinetics for growth . This threshold predicted the presence or absence of hyperthermophilic methanogens in various global hydrothermal systems based on H2 availability. However, a chemostat is necessary to estimate CH4 production rates by methanogens at different H2 concentrations. In this study, CH4 production rates and Monod kinetics at varying H2 concentrations were determined using a chemostat for Methanocaldococcus jannaschii and Methanothermococcus thermolithotrophicus. Arrhenius growth constants at varying temperatures were also determined using Balch tubes. These data were used to parameterize a reactive transport model describing the growth and transport of M. jannaschii and M. thermolithotrophicus beneath the seafloor, along with H2, heat, and Mg2+, from the high-temperature source fluid and diluted with seawater until the fluid reached the temperature observed on the seafloor. The model was applied to two physically distinct deep-sea hydrothermal vent sites at Axial Seamount. This work highlights the potential of coupling pure culture laboratory studies of environmental processes with reactive transport modeling and field observations to determine the size and nature of biogeochemical processes in environments that are otherwise difficult to access.
Methanocaldococcus jannaschii DSM 2661  and Methanothermococcus thermolithotrophicus DSM 2095  were purchased from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Braunschweig, Germany). For chemostat growth, M. jannaschii was grown at 82 and 65 °C and M. thermolithotrophicus was grown at 65 and 55 °C in a 2-L bioreactor with a working volume of 1.5 L. The growth medium was modified DSM 282 medium . The medium was reduced with 0.025% each of Na2S•9H2O and cysteine-HCl, stirred at 300 rpm, and sparged with 7.5 mL min−1 CO2 and varying flow rates of H2 and N2 to bring the total gas flow rate to 70 mL min−1, or to 100 mL min−1 for the highest H2 concentrations. The medium was maintained at pH 6.0 ± 0.1 by the automatic addition of 0.25 mM HCl.
The organisms were grown in batch phase prior to initiating the chemostat until they reached late logarithmic growth phase. The growth medium in the bioreactor was replaced with fresh sterile medium from an 18.5 L reservoir that was sparged with N2 and heated to the same temperature as the bioreactor. The dilution rate of the chemostat matched the batch-phase growth rate of the organism for the temperature and H2 concentration provided until steady-state conditions were reached (assumed to be after three full replacements of the medium based on longer test runs). The chemostat was operated such that all the H2 input into the reactor was consumed by the cells and the H2 output concentration was below the level of detection. Cell concentrations were determined using a Petroff-Hausser counting chamber and phase-contrast light microscopy. The headspace gas of the bioreactor was sampled directly using a Hamilton gas-tight syringe through a septum. The gas concentrations in the liquid medium were measured by anoxically transferring 20 mL of medium into a sealed 60 mL serum bottle that was pre-flushed with N2, allowing the gases to equilibrate into the headspace. The concentration of CH4 in the headspace was measured using a gas chromatograph (Shimadzu GC-17A) with a flame-ionization detector and a molecular sieve 5A column at 120 °C. The concentration of H2 was also measured using a gas chromatograph (Shimadzu GC-8A) with a thermal conductivity detector and an Alltech Haysep DB 100/120 column at 120 °C. The gas flow rate into the bioreactor was measured using a bubble meter. The cell-specific CH4 production rate was calculated from the sum of the CH4 concentration in the headspace times the gas flow rate and the CH4 concentration in the medium times the medium dilution rate, which was normalized by the total cell concentration in the reactor. Each measurement was taken in duplicate, and the dilution rate was changed midway through each chemostat run to determine the effect of changing the growth rate of the organisms on the rate of CH4 production.
To determine the effect of temperature on growth, growth rate was determined for cells incubated between 84 and 45 °C for M. jannaschii and between 68 and 30 °C for M. thermolithotrophicus. Cells were grown in modified DSM 282 medium as described above in Balch tubes sealed with butyl rubber stoppers and containing 2 atm (100 kPa added pressure at room temperature) of H2:CO2 (80%:20%) headspace. At various time points during growth, the cell concentration in the tubes was determined as described above. The growth rate was determined from a best-fit curve through the exponential portion of cell growth. The 95% confidence interval of the growth rate was determined using analysis of covariance (ANCOVA) .
A major challenge in developing the reactive transport model for methanogen growth is the lack of information on the size and configuration of the subsurface hydrothermal mixing zone. While numerical models using Darcy flow through a porous medium have captured the dominant features of hydrothermal circulation over the scale of an entire vent field or ridge system (i.e., 1–10 km) [25, 26], such models lack the resolution necessary to describe fluid flow at the scale of an individual vent, where flow is most likely controlled by the unique configuration of cracks and fissures feeding an outflow [27, 28]. Therefore, we took a simpler approach, whereby the flow was parameterized (rather than modeled using Darcy flow), but mass conservation was maintained.
The model describes the growth of methanogens in a high-temperature end-member fluid devoid of Mg2+ that is transported along a flow path and progressively diluted with seawater until it exits the seafloor. To represent fluid transport, the model considered a one-dimensional flow path consisting of a series of n boxes (Fig. 2). High-temperature hydrothermal fluid, lacking Mg2+, entered the first box with the fluid composition of the high-temperature end-member, then flowed from box-to-box, and at each box was progressively diluted with an equal amount of 2 °C seawater. Because the true length of the flow path is unknown, the model is non-dimensionalized with respect to space, such that the sum of all the box volumes is equal to one and represents the total volume of the subsurface mixing zone feeding the vent outflow. The total residence time of fluid in the system is set by the spatially non-dimensionalized fluid flux exiting the seafloor (Q′vt), which has units of time−1 and can be thought of as a dilution factor that defines the timescale of hydrothermal fluid circulation. The residence time fluid spends at different temperatures along the flow path is controlled by both Q′vt and the volume of each individual box along the flow path. To simplify the specification of box volumes, the volume of each box is given by the following formula:
where V is the box volume, n is the total number of boxes, x is a non-dimensional variable describing the position along the flow path and varies from 0 at the high-temperature end-member to 1 at the point where fluid is venting into the deep ocean, and xb is a shape parameter describing the geometry of the subsurface mixing zone. The shape parameter allowed the model to transition between two different mixing regimes. If xb >> 1, then the flow path resembled a linear crack or straight pipe (Fig. 2a). If xb << 1, then the flow path spread out as it rose, approximating an expanding plume percolating through the ocean crust (Fig. 2b). The fluid flux, Q′vt, and the shape parameter, xb, were treated as tuning parameters that were adjusted to understand how flow characteristics influence the chemical concentrations and the microbial populations present in venting fluids. While Q′vt sets the timescale of fluid flow, the shape parameter, xb, determined the relative amount of time spent at various temperatures along the flow path. All model parameters and boundary conditions are provided as Supplementary Material.
The model state variables were concentration of CH4, [CH4] (μmol kg−1), concentration of H2, [H2] (μmol kg−1), concentration of a thermophilic methanogen with M. thermolithotrophicus growth kinetics, [Mthe] (cells L−1), concentration of a hyperthermophilic methanogen with M. jannaschii growth kinetics, [Mjan] (cells L−1), concentration of Mg2+, [Mg2+] (mmol kg−1), and temperature, T (K). Temperature was calculated assuming pure mixing and was given by the following equation,
where fht is the fraction of high-temperature fluid in the box determine from the Mg2+ content of the venting fluid assuming 0 mmol Mg2+ kg−1 in the hydrothermal end-member, Tht is the temperature of the hydrothermal end-member fluid, Tsw is the temperature of seawater, and Cp,ht and Cp,sw are the heat capacities of hydrothermal end-member fluid and seawater, respectively. During hydrothermal circulation, Mg2+ is removed from solution within hours at high temperatures and the Mg2+ content of diffuse fluids indicates how much hot, zero-Mg2+ end-member is in the fluid .
The rest of the variables were described by the following system of differential equations for each box i, which were solved using the method-of-lines. The model was implemented in the R software environment using the package ReacTran .
where Q is fluid flux. Methane production (RCH4) is calculated by:
where vmax is the maximum rate of cell-specific CH4 production, KH2 is the half-saturation constant for cell-specific CH4 production, and Tmax is the optimum growth temperature of the methanogen. A conversion factor of 10−9 is used to convert νmax, which in Table S3 is expressed in terms of fmol cell−1 h−1 to μmol cell−1 h−1.
Growth rate for methanogens is given by,
where A is the Arrhenius constant and Ea is the activation energy.
Field constraints on the reactive transport model
Field measurements of Methanocaldococcus species, Methanothermococcus species, CH4, and H2 concentrations in exiting fluids at individual diffuse vents were used to constrain the modeled subseafloor methanogen abundance, CH4 production, and the shape function of fluid mixing for each vent. Dissolved inorganic carbon (DIC) was also measured in the fluids to ensure that it was not growth limiting. Thirty-seven diffuse hydrothermal fluid samples were collected from Marker 113 and Marker 33 (Fig. 1) in 2013, 2014, and 2015 using the deep-sea remotely operated vehicles Jason II and ROPOS (Table S1) as previously described [8, 12]. The concentration of Methanocaldococcus and Methanothermococcus cells in the diffuse hydrothermal fluids was estimated from the product of the proportion of these organisms in annotated metagenomic sequence read counts  and the total cell counts  (Table S1).
There was no high-temperature hydrothermal venting within 0.5 km of Marker 113 or Marker 33 that could provide hydrothermal end-member gas concentrations. Therefore, high-temperature end-member H2 concentrations for these sites were estimated based on a trend of end-member H2 and end-member Cl− for the closest high-temperature vents at Axial Seamount. The end-member Cl− concentration of Marker 113 is ~100 mmol kg−1 and that of Marker 33 is ~ 400 mmol kg−1 (from extrapolation of diffuse fluid Cl− to zero Mg2+). Temperature and chlorinity for the high-temperature end members were determined from the relationship of Mg2+ and temperature (or chlorinity) and extrapolated to zero Mg2+ concentration. The nearest high-temperature vents with similar Cl− end-members are Diva vent (end-member Cl− 200 mmol kg−1, end-member H2 400–970 µmol kg−1) and El Guapo vent (end-member Cl− 400 mmol kg−1 and end-member H2 120–470 µmol kg−1). End-member H2 concentrations at Marker 113 and Marker 33 were assigned values of 950 and 300 μmol kg−1, respectively. These end-member H2 values for the diffuse vent sites reflect the fact that Marker 113 has a vapor-dominated source with higher gas content than the source for Marker 33.
H2 Monod kinetics for methanogenesis
M. jannaschii was grown in 11 separate chemostat runs and M. thermolithotrophicus in 9 separate chemostat runs at varying H2 concentrations, dilution rates, and temperatures (Table S2). The cell-specific CH4 production rates were not distinguishable based on dilution rate or growth temperature at each H2 concentration examined, so the Monod kinetics data were pooled for each organism (Fig. 3a). For M. jannaschii, the maximum CH4 production rate (Vmax) was 43 ± 5 fmol CH4 cell−1 h−1 (± standard error) and the KS for H2 was 37 ± 15 µM. For M. thermolithotrophicus, the Vmax was 24 ± 3 fmol CH4 cell−1 h−1 and the KS for H2 was 27 ± 12 µM. The growth rates of both organisms in a closed system (Balch tubes) were measured over their growth temperature range to determine their Arrhenius constants (Table S3). For M. jannaschii, the activation energy (Ea) was 86.3 kJ mol−1 and the pre-exponential factor (A) was 9.16 × 1012 h−1. For M. thermolithotrophicus, the Ea was 73.8 kJ mol−1 and A was 3.36 × 1011 h−1 (Fig. 3b).
Reactive transport modeling
The parameter Q′vt set the timescale or average residence time of fluid circulation through the hydrothermal system. If this parameter was large, then the residence time was short and there was not enough time spent at optimal growth conditions for a population to develop. Any microbes that might be present were simply washed out and H2 and CH4 followed conservative mixing between high-temperature source fluid and seawater. If Q′vt was small, then the residence time was long and there was enough time for methanogen populations to establish in the fluid during its transport through the system. Any H2 present in the fluid was converted to CH4, producing significant H2 and CH4 anomalies from conservative mixing.
The pipe-like and expanding-plume models shown in Fig. 4 had identical final H2 and CH4 concentrations (Fig. S1), but the ratio of hyperthermophilic-to-thermophilic methanogens varied significantly. For fluid flow through a straight-pipe-like model, the hyperthermophile Methanocaldococcus dominated the system by consuming all the H2 prior to any significant growth of the thermophile Methanothermococcus (Fig. 4a). Fluid flow was constrained by the walls of the pipe, so as seawater was entrained into the pipe, mass conservation accelerated the flow, leaving the thermophiles more sensitive to washout than the hyperthermophiles. Alternatively, if the cross-sectional area increases along the vertical flow path, as in the expanding plume model, the thermophile Methanothermococcus dominated the system (Fig. 4b). The exact transition between the dominance of the two types of methanogens depended on the residence time, composition, and temperature of the end-member fluids.
The reactive transport model was applied to two sites of diffuse venting at Axial Seamount. The aqueous H2 and CH4 concentrations in exiting diffuse fluids were 0.1–2.6 μmol kg−1 of fluid and 13–40 μmol kg−1 of fluid at Marker 33 and Marker 113, respectively, indicating H2 consumption and CH4 production relative to conserved end-member mixing . The DIC concentrations were 4.5–15.1 mmol kg−1 of fluid at these sites and were not considered limiting to methanogenesis. At Marker 113, 15–31% of annotated metagenomic sequences were assigned to known methanogenic genera, primarily thermophilic Methanothermococcus species, based on metagenomic and culture-dependent analyses (Table S1) [8, 12]. The estimated concentrations of Methanothermococcus and Methanocaldococcus cells in exiting vent fluids based on metagenomic analyses were 1.9 ± 0.7 × 108 cells L−1 (± standard error) and 2.9 ± 0.9 × 107 cells L−1, respectively. In contrast, at Marker 33, only 2–5% of annotated metagenomic sequences were assigned to known methanogenic genera, primarily hyperthermophilic Methanocaldococcus species (Table S1) [8, 12]. The estimated concentrations of Methanothermococcus and Methanocaldococcus cells in these exiting vent fluids, based metagenomic analyses, were 1.2 ± 0.3 × 106 and 1.2 ± 0.3 × 107 cells L−1, respectively.
To fit the model, both the fluid flux (Q′vt) and shape parameter (xb) were adjusted until the H2 and CH4 concentrations and ratio of thermophiles to hyperthermophiles fit those observed in the vent fluid outflow (Fig. 5). The model closely reproduced the H2 and CH4 concentrations observed and methanogens estimated in the vent fluid outflow. For Marker 113 and Marker 33, the residence times of fluid below the surface were 33 and 29 h, respectively. Parameter values and boundary conditions used for the simulations are provided in Table S4.
Methanocaldococcus and Methanothermococcus species almost exclusively use H2 and CO2 as their carbon and energy sources. Some Methanothermococcus spp. can use formate in lieu of H2 and CO2 [23, 31], but microcosm enrichments using diffuse fluids from Axial Seamount that were spiked with formate or acetate instead of H2 did not result in CH4 production at 55 or 80 °C . These attributes make these methanogens amenable to modeling based on H2 availability. Previous Monod kinetics determined for Methanocaldococcus spp. in a batch reactor generally predicted which hydrothermal vent fluid chemistries could support the reproduction of these methanogens . However, growth and CH4 production in a chemostat at constant H2 flux rates were needed to estimate biomass production and the biogeochemical impact of high-temperature methanogens in the subseafloor. This study expanded the temperature range of these measurements by including a Methanothermococcus species. The KS and minimum H2 threshold for CH4 production measured in this study in the chemostat for M. jannaschii and M. thermolithotrophicus were slightly lower than the growth kinetic values measured previously for three Methanocaldococcus species grown in a batch reactor .
Comparing the fitted model domains for Marker 113 and Marker 33, the shape function for Marker 113 was more expanding plume-like while that for Marker 33 was more pipe-like. Although the average residence time of hydrothermal circulation was similar for both vents, the local residence time in the optimal growth ranges for Methanothermococcus and Methanocaldococcus differed due to the hydrology of the flow paths. The plume-like hydrology of Marker 113 resulted in a shorter residence time for hotter fluid compared to cooler fluid, favoring Methanothermoccus, while the pipe-like geometry of Marker 33 had a longer residence time at the hotter temperatures favoring Methanocaldococcus. This trend is similar to that observed for these two vent sites using metagenomic analyses, where mesophilic Epsilonbacteraeota were predominant at Marker 113 while thermophilic Epsilonbacteraeota were predominant at Marker 33 .
The reactive transport model was non-dimensional, which permitted estimates of residence times but not the extent or volume of the subsurface biotope associated with each vent. If fluid flux estimates are known, then the model could be rescaled to estimate the subsurface volume and methanogen abundance (details of these calculations are described in the Supplementary Material). While diffuse fluid flux measurements at Marker 113 and Marker 33 are not available, Pruis and Johnson  used a hydrologically-sealed sampler to estimate the diffuse fluid flux in the ASHES vent field at Axial Seamount (Fig. 1), ~2 km from Marker 113 and Marker 33, where fluids emanate from a collection of similarly small (<5 cm width) fractures. The fluid flux estimate was 48 m3 m−2 y−1. Assuming this fluid flux rate for Marker 113 and Marker 33, our model predicted that each vent hosts 0.4–1.3 × 1011 methanogen cells m−2 of vent seafloor surface area (Table S5). Assuming an effective porosity of 10–30% , the methanogens would occupy only 1.8–18 m3 of ocean crust m−2 of vent seafloor surface area (Table S5). From this, the depth below the seafloor at which methanogen growth begins can be estimated (see Supplemental Materials). For Marker 33 where xb = 100, methanogen growth begins 1.8–18 mbsf depending upon the porosity. For Marker 113 where xb = 1, methanogen growth begins somewhere between 3 and 28 mbsf (Fig. S2). This result was consistent with previous field observations suggesting that the subsurface populations feeding these vents are predominately local features [12, 22, 33] drawing on nearby subsurface microbial populations, rather than large-scale subsurface hydrothermal fluid circulation patterns. The degree to which these local hot spots of microbial activity represent the overall subsurface biotope of Axial Seamount is unknown.
The 29–33 h subseafloor residence times of the diffuse fluids at Axial Seamount were comparable to the 17–41 h resident time estimate for microbes in a diffuse vent Crab Spa at 9°50ʹ East Pacific Rise hydrothermal vent site . We similarly found that a relatively small but highly productive microbial standing stock in the subseafloor at vents likely accounts for the biogeochemical alteration and biomass output from subseafloor vent chemoautotrophs. Unlike Crab Spa where there was net CH4 consumption in the diffuse fluids , there was net biogenic CH4 production in our two diffuse vent fluids at Axial Seamount, demonstrating that in certain circumstances methanogens can represent a significant proportion of the total primary productivity.
One possible caveat for our model was that it only considered microbial growth in the fluid phase and not growth of microbes attached to surfaces. Hyperthermophiles, including M. jannaschii, produce biofilms and form attachments to hydrothermal minerals [34, 35]. The microbial mat and centimeter-scale flocculent material that was flushed from the seafloor immediately following a volcanic eruption at Axial and elsewhere [10, 36, 37] suggests that subsurface microbes attach to one another and to solid surfaces, most likely to prevent washout from the system. Incorporating biofilms into the model introduces additional parameters, such as the attachment strength and surface area available for colonization. For the Marker 113 model, the CH4 anomaly observed in the diffuse fluid matched the concentration of methanogens present in the vent outflow. This suggests that the methanogen population at this vent could exist in the fluid phase given the estimated residence times of the fluid at thermophilic growth temperatures. In contrast, the number of hyperthermophilic methanogens needed to produce the modeled CH4 anomaly at Marker 33 was much higher than the numbers observed in the diffuse fluid outflow (Fig. 4b). The ‘missing’ hyperthermophilic methanogens could be living in a subsurface biofilm.
Another possible caveat for our model is that it assumes a constant growth yield (i.e., amount of cell mass produced per mol of CH4 produced) across varying H2 concentrations and temperatures. In this study, M. jannaschii and M. thermolithotrophicus were grown in the chemostat at maximum cell concentrations such that all the available H2 in the reactor was consumed, which closely resembles the complete depletion of H2 in diffuse vent fluids exiting the seafloor. However, when M. jannaschii was grown in a chemostat at 80–83 μM H2 and 10-fold lower cell concentrations such that excess H2 was observed in the headspace, the growth yield (Yp/CH4) dropped ~10-fold and the cell-specific CH4 production rate increased to ~ 500 fmol CH4 cell−1 h−1 . Growth yields also increased in laboratory studies in the hydrogenotrophic thermophile Methanothermobacter thermoautotrophicus and the mesophile Methanococcus maripaludis when H2 was limiting [39,40,41]. Therefore, the ‘missing’ hyperthermophilic methanogens at Marker 33 may also be due to excess H2 availability and low hyperthermophilic methanogen growth yields at hyperthermophilic temperatures that lead to much higher CH4 production rates per cell. In this study, the cell-specific CH4 production rates are conservative and the estimated number of total methanogens at each Axial vent in this study should be considered upper limits. This means that the population of methanogen cells necessary to support substantial methane anomalies could be even smaller than we have predicted.
This study used two representative methanogens as tracers of subsurface fluid circulation and microbial production at two diffuse vents at Axial Seamount. By combining laboratory-derived methanogen growth kinetics with reactive transport modeling and in situ observations, the population size of methanogens, the volume of crust occupied by these organisms, the fluid residence time, and the nature of subsurface mixing were estimated. Results suggest that the methanogen population at each vent was relatively small and local, occupying as little as 2 m3 of subsurface crust and consisting of 0.4–1.3 × 1011 total methanogen cells m−2 of vent area. The model showed that the differences in the methanogen populations at Marker 113 and Marker 33 can be explained by differences in the geometry of the subsurface hydrology. Therefore, small-scale variation in the geologic fabric of the upper crust can create varied fluid flow paths fixed in space that harbor persistent and distinct microbial communities, whose metabolic activity produces microbial and chemical signatures at seafloor vents.
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We thank Susan Merle, Andra Bobbitt, and Bill Chadwick for providing the site map. We thank the captains and crews of the R/V Falkor, R/V Thompson, and R/V Brown as well as the ROV ROPOS and JASON groups. Lisa Zeigler Allen, Giora Proskurowski, Kevin Roe, and Begüm Topçuoğlu provided critical support in sample collection and experimental design and execution. This is C-DEBI contribution number 464.
This work was funded by the Gordon and Betty Moore Foundation grant GBMF 3297 to JAH, JFH, JJV, and DAB; the NASA Earth and Space Science Fellowship Program grant NNX11AP78H to LCS and JFH; NSERC Discovery grant to CKA, a Fulbright New Zealand-Ministry of Research, Science and Technology Graduate Award to LCS; NSF grant OCE-1547004 to JFH, NSF Grant OCE-1546695 to DAB, NSF grants GEO-1451356 and GEO-1238212 to JJV, the NSF Center for Dark Energy Biosphere Investigations (C-DEBI) grant OCE-0939564 to JAH, and by University of Washington Joint Institute for Study of the Atmosphere and Oceans, NOAA Cooperative Agreement NA15OAR4320063. The data collected in this study includes work supported by the Schmidt Ocean Institute during cruise FK010-2013 aboard R/V Falkor.
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Stewart, L.C., Algar, C.K., Fortunato, C.S. et al. Fluid geochemistry, local hydrology, and metabolic activity define methanogen community size and composition in deep-sea hydrothermal vents. ISME J 13, 1711–1721 (2019). https://doi.org/10.1038/s41396-019-0382-3
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Taxes are among life’s couple of constants. Every year, the substantial majority of Americans are needed to file an income tax return with the Internal Revenue Service (Internal Revenue Service). Even though “tax season” has become an indispensable part of American life, the process included could still be complex or even overwhelming for lots of Americans. The intricate nature of federal tax obligation regulation is, partly, to blame for the confusion. To give you a better concept of exactly how tax legislation works, this section gives a variety of posts as well as resources on federal tax, tax forms, audits, as well as tax troubles. You’ll locate detailed details on filing your return, asserting exemptions, taking advantage of deductions, taking care of an audit, avoiding tax problems, as well as obtaining lawful help with your tax obligations.
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Technological advancements are reshaping the world and becoming more critical than ever. The transformation in design, robotics and sustainability has profoundly changed the way economies operate and the dynamics in which people, society and environments inter-relate. We have experts from academia as well as industry to share with you the latest technological advancements in these fields.
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Technology and Innovation
The future of the German economy lies in your technology and innovation potential. The transfer of knowledge into competitive products and services is therefore a major focus for the Nueremberg Chamber of Comerce for Central Franconia. The technology transfer will be successful if new technical ideas can be fleshed out further. Additional performance criterion the economic valorizing of research results.
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On Thursday, January 28, CEO Doug Parker and President Robert Isom hosted the fourth-quarter and full-year 2020 State of the Airline webcast. Watch the videos of the live webcast below.
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domain: news


text:
Rock and Roll, Over the Hills and Far Away, Black Dog, Misty Mountain Hop, Since I've Been Loving You, Dancing Days, Bron-Y-Aur Stomp, The Song Remains the Same, The Rain Song, Dazed and Confused, Stairway to Heaven, Whole Lotta Love, What is and What Should Never Be
Plant skips a line during the final verse of Rock and Roll and ends up ahead of the band for a few bars before getting himself back on track. Page shreds through the guitar solo during Black Dog. Since I've Been Loving You features an aggressive guitar solo from Page. The Song Remains the Same is played at a frenzied pace. Bonzo attacks his drums with incredible force during the initial verses of Dazed and Confused. The lead-in to the bow solo features an excellent call and response between Plant and the rest of the band, followed by one of the best renditions of San Francisco thus far. Page's fingers fly across the fretboard at lightning speed during the amazing guitar solo/workout section. A truly epic performance.
Plant's voice is incredibly powerful during Stairway to Heaven. Whole Lotta Love is preceded by a fantastic intro reminiscent of The Who. The Everybody Needs Somebody to Love section begins with a cacophonous explosion. The medley includes Boogie Chillen', Elvis Presley favorites (You're So Square) Baby I Don't Care and Let's Have a Party, I Can't Quit You Baby, and Goin' Down Slow. Unfortunately, the tape suffers from major disturbances for the remainder of the show beginning during the blues improvisation. Page blazes through the solos during Heartbreaker. Bonzo pummels his drums during the final verse. The band closes the show with what will prove to be the final recorded performance of What is and What Should Never Be. Plant ends the night in good form, regaining some of his former glory. He leaves the crowd with a simple "thank you very much... see ya."
The tape is a bit hissy, distant, and lacking bottom end. Unfortunately, the quality deteriorates drastically toward the end of the show.

domain: artistic


text:
I am currently in my 3rd year of teaching elementary art. I currently teach K-2 and I have about 440 students. Though my degree is in general ed, I have always been an artist outside of my studies. I have illustrated 2 children's books, and I do commission pieces outside of school.
One of my main goals is to build relationships with my all of my kiddos in some way. I think creating projects that interest them and get them excited is a great way to do that. I also have high expectations of my students and we can create some challenging artwork; however, I compensate the difficulty level with the pace at which we complete a project. I like to break each project down to its simplest parts. Since I only have my kids for 30 min. each class period, this is a necessity. I like to keep things short, sweet, and to the point so that my kiddos have more time to create! We may do fewer projects than other art classes do, but our stuff is really cool and complex most of the time - I am proud of that.
Yet to be added
I grew up in the Beech Grove city school system (just outside Indianapolis, IN) from K-12. It wasn't until about 3rd grade when I realized that I had a passion/skill for the arts. I was fortunate enough to have such wonderful art teachers that helped me reach my potential. I was originally going to major in studio art, but I was not sure what I was going to do after college. I eventually transferred from the University of Indianapolis (Crystal Dehaan School of Art) to Indiana University Southeast, and I switched my major to general education. I graduated in May 2016 with my degree. I had a hard time finding gen ed teaching jobs that summer, so I thought I would apply to be an art teacher. The rest is history! I love my career and I feel so blessed to teach kids about art.
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text:
I try to keep up with metal and heavy rock internationally, but it was nice to get review material from an Iraqi band. Let it not be said I'm not open to metal from all lands.
Acrassicauda: Only the Dead See the End of War
Metal bands love writing about death and war. However, very few bands will be able to call on experiences of actual war to color their lyrical output. This lot, the subject of the documentary Heavy Metal in Baghdad, have seen enough to power the minds of scores of metal bands. And if you want to talk persecution and angst, they have seen their share of that too. They had to flee to the U.S. to pursue their preferred craft.
While only an EP, it’s a pretty tight affair. This is not surprising as it's produced by Testament/Alex Skolnick Trio man Alex Skolnick. There are various influences here from Megadeth on “Message from Baghdad” to something a bit more modern on “The Unknown”. Not one of these tracks disappoint despite their eclectic nature. It’s a release where the band is exploring their influences and finding their feet.
A compelling biography and some damn find metal really combine to quite an interesting package. With their next release, presumably a full one, they will have to emerge from their saga to play in the big leagues. On the strength of this release, they are certain to have a really good crack at it. Certainly a band to watch.
At Vance: Ride the Sky
This band are that European brand of metal that is highly polished, musically proficient, and terribly catchy. For proof, just listen to the soaring track “Last in Line.” It hits all the right triggers over the course of its length. Some might quibble about the pretentiousness of including an instrumental track called “Vivaldi” but that would be petty.
Rick Altzi has a Jeff Scott Soto quality about his voice and successfully avoids the pronunciation pratfalls you sometimes find with this sort of band. His voice is perfectly matched to the soaring guitar widdling of Olaf Lenk. In fact, the only criticism I could muster for this release is the rather pointless, if passable, cover of Bad Company’s “Wishing Well”. That said it could so easily be butchered and the band pull it off with aplomb. This lot don’t need to do covers any more so it’s a bit of waste.
If you like sophistication and quite a bit of melody in your power metal, than this band will work nicely. The mixture of AOR, power metal, and classical sensibilities works well. If you like the early days of Rising Force before Malmsteen went a bit off the boil, look no further. Over look the rather cheesy name and enjoy the music.
Raise the Red Lantern: s/t
These guys don’t do subtle that is for sure. They named their last album Thunderfuck and this release carries on that tradition. There isn't an over the top title like the first one, but they do let their music to the talking. They are made up of three guys who build custom speaker cabinets used by bands like Pelican and their ilk and anther who is part of the metal progressive jazz band Yakuza that has been amazing and confusing its listeners for years.
Similar to bands like Baroness and other purveyors of metal stoner doom, this is heavy stuff played with lots of attitude and angst. It's an amalgam of all the possible incarnations of doom metal from the modern to Black Sabbath and back again. You never know what is round the corner, but you are sure keen to get there.
Ultimately this sort of stuff is love or hate music. And you will probably not be sure how you feel depending on the day you listen. Deep, heavy riffing sludge that rewards the listener if you give a chance. Although it might be a bit of hype, this band does smack of one that you will be telling people you knew them once they hit the big time.
Okay, it's hard to tell what the hell this release is or what genre it falls under. One track would work great at a roller disco, “Strut (Disco Ball Mix),” with its naturally 70s disco vibe tinged with nice guitar riffing. This is an in-house re-mix done by Andy Selway of the band. Next thing you know it's some uber-heaviness care of the nuts at Prong. Ivan de Prime of White Zombie shows up for a mix of “Never say Never”. This is also a track that is tackled by Komar Kommando whoever he is, needless to say they end up quite different. Oh yeah and “Rave” Oglivie also takes a crack at the track.


text:
Yesterday, I explained the basic instructions for decorating your journal. In this article, I’d like to focus on the extra touches you can do to make the album a personal reflection of you, and just to make it stand out.
I created two recently.
The first, shown at left, was a composition notebook that I covered with two different sheets of patterned paper. I chose a dark green patterned paper, to accompany the aged tan paper with the leaves all over it. I immediately loved the look, but then I am very drawn to autumn and fall colors. I decided to rip the paper so I could layer it and give it a semi-rustic look. Although I plan to do more to the cover, at this time I haven’t gone much further.
I did however decide to create a bookmark in the book (as shown on right). I used a piece of dark green fiber, though you could use ribbon, yarn, string or anything else. I actually chose the green fiber because I liked the prickly way it looked coming off the side of the book – almost like pine needles, giving the album still more of that nature feel to it.
I wanted something to be hanging off the end of the bookmark, and at first had decided on some beads. Then I came across the leaf you see pictured and I was sold. It was exactly what the album needed. And was a wonderful lesson on why if something doesn’t feel right, leave it and come back to it. If I had done the beads, and found this later, although I could have probably changed it, it would have been a hassle.
It’s very easy to place a bookmark into your album. Run the length of your fiber, ribbon, etc. along the inside of the front cover, all the way to the top of the outside of the spine. Leave enough to tie in place at the top corner of the book. Next you’re going to continue with the fiber, ribbon, etc, and run it down the center of the book. Just flip it open to whatever page it comes to and lay the string down through the center. You’re going to want some hanging out of the bottom of the book because then you know which page you’ve marked. Cut the piece with about an inch to an inch and a half hanging out. Now tie the fiber at the top leaving that piece hanging. If you want to secure beads, charms, or whatever else you can find to the bottom, do that now.
Now you have a built in bookmark for your journal.
At the same time I created the journal above, I also made another one. This one I’ve embellished a bit more.
I started with a gorgeous black and white patterned paper. It had texture in the paper already, with white roses, and torn paper and tinsel. It’s a beautiful paper. I decided to add some word ribbons, some hearts and some letters that spell out DREAM. I then glued a length of tiny pearls along the spine of the book securing in place at the bottom of the book. This is a different technique than the bookmark. After I secured it at the bottom, I attached a gold charm that reads “Life is measured by moments”. I then snipped the pearl strand so both sides were even.
I love the elegant way this journal looks. It’s not even finished yet, either. I’d like to paint or do something with the big white heart on the left side.
Of course now my four girls want to create journals of their own. Guess what we’re doing this weekend?

domain: hobby


text:
“But I know that I’ll be happier, and I know you will, too.”
It was a volunteer colleague by the name of Kevin who first planted the name “Tame Impala” into my brain, way back several years ago at one of our organization’s famous Han Dynasty fundraiser dinners. Not quite sure what album he was playing from then, but it didn’t fully stick until another friend of a great many years, Krystal, posted Feels Like We Only Go Backwards, from the Lonerism album, onto our facebook feed. That sort of propelled Tame Impala to one of my top bands of the year for 2013. I did even get to see their mind-blowing live show at the Electric Factory with my cousin, Angie. To say that 2015’s follow-up was long anticipated would be an understatement.
When I did finally get a hold of Currents, I wasn’t immediately enamored. I loved Lonerism because it reinvented classic rock guitar psychedelia for the 21st century. Currents was a deliberate departure from that groove to more of an electronic sound painting. Pitchforks notes more than once in its 2015 review the “near total absence” of guitars. That’s not a direction I would generally favor, although I do appreciate the adventurism.
When I buy a band’s new album, I do want it to sound substantially like the old album—but I also want to hear something new. It’s a tough balance for any band to strike. I’d have to say that I appreciate a band’s attempt to evolve over stagnating, even if I’m not fully on board with the direction they’ve chosen to travel. And there are elements of Currents that reward repeated listenings, especially in a small room with four speakers and two subwoofers. It’s a fine album, even though for me personally it did not deliver what Lonerism did.


text: A very interesting book that will appeal to anyone who has ever looked at an old ship and wondered what stories it had to tell. I agree that the focus and tone of this book are satisfying -- it is far more an emotional journey through the rusting relics of history than it is (or, in my opinion, should be) a review of the policy or technology of the mothball fleet. The author's obvious love and respect for these ships gives the book its considerable appeal. However, I opted for four stars instead of five because I thought the organization of the book was a little shaky, moving from the end of World War II; through the mothballing process; to later conflicts and reactivation; then back to World War II through the ship's histories. I also would have enjoyed more photo coverage, including some pictures of the interiors of mothballed ships (the author makes several, tantalizing remarks about the interiors of the mothballed being time capsules of the end of World War II) and pictures of the ultimate fates of mothballed ships (photos of the scrapping of the Enterprise and the listing, rusted hulk of the light carrier Cabot would enhance this book's wistful view of the subject). These thoughts aside, it was a very good book that made me fondly recall looking at one of the mothball fleets years ago with my father.

domain: library


|Grade||Genuine Rolex||AAAAA Clone||AAA Replica||A Replica|
|Movement||Rolex Calibre 3285||Genuine Swiss Clone 3285 movement||Swiss 2836 Movement||Asian 2813 Movement|
|Time Accuracy||-3/+5 sec per day COSC certified||-5/+6 sec per day COSC certified||-10/+10 sec per day COSC certified||-1/+2 min per day COSC certified|
|Stainless Steel Type||904L Stainless Steel||904L Stainless Steel||316L Stainless Steel||316L Stainless Steel|
|Bezels||Bi-directional Rotating||Bi-directional Rotating||Fixed||Fixed|
|Crystal||Sapphire crystal||Sapphire crystal correct date magnification||Sapphire crystal||Mineral Glass|
|Logo & Markings||laser etchings||Exact on entire watch||90% Exact on entire watch||70% Exact on entire watch|
|Dial||Genuine Rolex||Exact detail and font||90% exact detail and font||70% Exact detail and font|
|Luminous Markers||Rolex Lumen||SuperLuminova Swiss Lumen||Swiss Lumen||Normal Lumen|
|Waterproof||100m on standard models||Tested up to 300m||Tested up to 30m||Life waterproof|
|Life expectancy||Lifetime||Lifetime||3-5 years||1-2 years|
|Warranty||2 Years||Up to 5 Years||Up to 3 Years||Up to 1 Years|
|Engine||Rolex Calibre 2836/2813|
|Series||Gmt Master Ii|
Clasp: Fold Over Clasp. The clasp design of the watch allows the user to fold it into several positions or angles for unique and unique use.
Dial Markers: luminous dot. Many people can’t tell the time in the dark. This watch has a luminous spot, which is the eye of the watch, which can help you see the time even in the dark. It is an advanced color sensor that can detect light levels of 2.5 lux or less with a range of up to 100 meters.
Second Markers: Minute Markers around the outer rim. The second mark on the watch is called the hand of the clock. This design element is used in many famous designs such as clocks, clocks and other objects.
Band Material: 316L. The situation is 316L stainless steel, which has very good corrosion resistance and good texture.
Case Back: Solid. The sturdy back cover is the “second skin” of the watch. It covers metal and provides daily wear protection for the watch.
Watch Case: 316L. The case is made of 316L stainless steel, making it durable.
Function: Date, Hour, Minute, Second. The watch has many functions. The watch is equipped with a indicate date to measure time, the minute dial can estimate the current time, and the second dial can estimate the time after the interval.
Luminiscence: Hands and Markers. About the luminosity of the watch is hands and markers. With this watch, you will not lose your time in dark.
Water Resistance: 30 meters. The watch’s waterproof depth is 30 meters, which is similar as real watch. Tips: The A Replica is just daily waterproof, need to purchase additional waterproof service which is up to 30 meters.
Crystal: Sapphire Crystal. Sapphire crystal watches are a very common glass that can protect your watch from scratches. Tips: The A Replica is applied with mineral glass instead of sapphire.


text:
Monosodium Glutamate (MSG) causes Mental Disorders.
Glutamates are involved in epilepsy, schizophrenia and cognitive disorders (memory and learning).
Monosodium Glutamate (MSG) is a natural salt of glutamic acid. MSG is used as a natural food additive to enhance flavors of industrialized food.
Currently, MSG is added to candies, chewing gum, seasonings, catsup, English sauce, soy sauce, all other sauces, gelatins, yeast extracts, calcium caseinate, soups, fruit drinks, thirst-quenchers, beer, corn gluten, almost all snacks, tofu, soy milk, industrial milk, low fat milk, cheeses (in particular parmesan cheese), soaps, shampoos, hair conditioners, cosmetics, medications, chicken pox vaccine, etc. At present, MSG is added to almost all industrially produced foods, including food for children.
But, why this scandal has grown around Glutamates?
We would not say it, but, recently, serious research companies have realized that Glutamates cause severe human degenerative diseases, schizophrenia among them.
Many scientists have informed that the platelet sensitivity to glutamate is increased in people with a tendency to nervous depression. (Berk, M., H. Plein & D. Ferreira. 2001. Platelet glutamate receptor supersensitivity in major depressive disorder. Clin. Neuropharmacology. 24: 129-132).
Several clinical studies demonstrate that the glutamates can cause severe nervous depression and schizophrenia. (Ian A. Paul and Phil Skolnick. Glutamate and Depression. Clinical and Preclinical Studies. Ann. N.Y. Academy of Sciences. 1003: 250–272. 2003).
Glutamates can cause nervous depression and suicidal proclivity, mainly in young people. (Gerard Sanacora, Douglas L. Rothman, Graeme Mason and John H. Krystal. Clinical Studies Implementing Glutamate Neurotransmission in Mood Disorders. Ann. N.Y. Academy of Sciences. 1003: 292–308. 2003).
The Society of Neuroscience has established that "Unquestionably, Glutamates, at doses encountered in products, cause severe injury to hypothalamus, the essential part of the brain for both memory and learning".
Recently, Bita Moghaddam and Barbara W. Adams, of the Department of Psychiatry in Yale University School of Medicine, reported irrefutable evidence on the involvement of Glutamates in schizophrenia.
The FDA has classified glutamates in the Generally Recognized as Safe (GRAS) category of additives. FDA bases its statement on a non-scientific report, published in 1971, whose falsehood has been repeatedly verified and exposed publicly. In 1971, Dr. W.A. Reynolds and colleagues published an article where they claimed that big doses of MSG supplied to baby monkeys "had no toxic effect" on infant monkey Central Nervous System. The scientific community doubted of Reynolds' study because in Dr. Olney's practice, large doses of MSG fed to animals at all times caused the animals to vomit. Dr. Reynolds et all did not mention any vomiting in their testing. If there were actually vomiting, it would nullify the Reynolds' study because MSG would not have been metabolized. Something is going wrong.
IN THE MEANWHILE, I WILL AVOID TO INCLUDE THESE "GRAS" IN MY NUTRITIONAL REGIME, AND PARTICULARLY IN THAT OF MY CHILDREN. NO ONE CAN CONTROL MY DETERMINATION ON CHOOSING THE HEALTHIEST FOOD THAT I AND MY CHILDREN SHOULD CONSUME.
Berk, M., H. Plein & D. Ferreira. 2001. Platelet glutamate receptor supersensitivity in major depressive disorder. Clin. Neuropharmacology. 24: 129-132.
Ian A. Paul and Phil Skolnick. Glutamate and Depression. Clinical and Preclinical Studies. Ann. N.Y. Academy of Sciences. 1003: 250–272. 2003.
Gerard Sanacora, Douglas L. Rothman, Graeme Mason and John H. Krystal. Clinical Studies Implementing Glutamate Neurotransmission in Mood Disorders. Ann. N.Y. Academy of Sciences. 1003: 292–308. 2003.
Glutamate and Disorders of Cognition and Motivation; 13 to 15 April, 2003; sponsored by the New York Academy of Sciences.
Kohichi Tanaka, Kei Watase, Toshiya Manabe, Keiko Yamada, Masahiko Watanabe, Katsunobu Takahashi, Hisayuki Iwama, Toru Nishikawa, Nobutsune Ichihara, Tateki Kikuchi, Shigeru Okuyama, Naoya Kawashima, Seiji Hori, Misato Takimoto, and Keiji Wada; Epilepsy and Exacerbation of Brain Injury in Mice Lacking the Glutamate Transporter GLT-1; Science 1997 June 13; 276: pps.1699-1702.
Guochuan E. Tsai, William E. Falk, and Jeanette Gunther; A Preliminary Study of D-Cycloserine Treatment in Alzheimer's Disease; J. Neuropsychiatry. Clin.
Neurosci., May 1998; 10: 224 - 226.
Moghaddam, Bita and Adams, Barbara W.; Reversal of Phencyclidine Effects by a Group II Metabotropic Glutamate Receptor Agonist in Rats. Science 1998 August 28; 281: 1349-1352.

domain: medical


text:
Joël Robuchon’s two exceptional French restaurants reopen in July on the Las Vegas Strip
After closing in March 2020 when the state closed non-essential businesses, including restaurants and casinos to prevent the spread of COVID-19, two of the city’s most beloved French restaurants are set to reopen. JoÃ«l Robuchon returns on July 1 at MGM Grand and the more relaxed L’Atelier de JoÃ«l Robuchon workshop reopens on July 15.
L’Atelier de JoÃ«l Robuchon offers diners a front row seat when they sit down at the bar overlooking the kitchen to observe the artistic talent of the chefs. Robuchon’s more laid back approach to dining features tapas, a tasting menu, and wines from around the world. Customers can even order Robuchon’s vision of a burger and sample his famous mashed potatoes.
Jonathan Doukhan, executive chef of L’Atelier de JoÃ«l Robuchon, explains that his team has spent the last year and the change coming up with new dishes that transform âcommonly used ingredients into dishes never seen before in a Robuchon restaurant or elsewhere. The menu is both recognizable and accessible for newcomers, as well as a stimulating challenge for foodies. Saint Pierre features Saint Pierre Ã la plancha on a “sambol” of tomato, candied lime zest and coriander puree; The Grenouillle is served with caramelized frog legs on a creamy spelled risotto; and La Framboise brings a sphere of white chocolate with a fresh raspberry and yuzu ice cream.
Diners can sit next to Chuck Norris, one of the celebrity photos that line the richly decorated space of the great JoÃ«l Robuchon that looks like a cross between the inside of a FabergÃ© egg and Marie’s boudoir -Antoinette. Robuchon’s deliciously elaborate dishes are making a comeback, including the 15-course tasting menu. It is French grandeur that meets Asian refinement. Ravioli of langoustines truffled in a foie gras sauce, a semi-soft boiled egg on a spinach purÃ©e and a Maine lobster in a minced turnip join the carts of cheese, bread and mignardises. Sit in the lavish front lounge for a glass of champagne.
Christophe de Lellis, executive chef of JoÃ«l Robuchon, says his team has been working on a menu of beloved dishes for the comeback, like Le Caviar ImpÃ©rial with Osetra caviar served over king crab in a shellfish jelly sprinkled with mashed potatoes. cauliflower; Langoustine with truffled langoustine ravioli served with a simmered cabbage and foie gras sauce; and La Rosa, a Dassai â39â sake jelly topped with a white chocolate rose and orange coulis.
Both restaurants were part of Eater 38 when they were open.
Robuchon died in August 2018 at the age of 73 from cancer. Robuchon obtained the title of Chef of the Century from Gault Millau in 1987.
â¢ JoÃ«l Robuchon’s two stellar restaurants will return âwhen activity levels allowâ [ELV]
â¢ All reports by JoÃ«l Robuchon [ELV]
â¢ All the cover of L’Atelier de JoÃ«l Robuchon [ELV]
â¢ How the coronavirus is affecting food and restaurants in Las Vegas [ELV]


Wednesday, May 30, 2018 | 6:30 p.m.
The first marijuana dispensary to have its license suspended by the Nevada Department of Taxation had that ban lifted Wednesday afternoon and was prepared to reopen.
Pisos, 4110 S. Maryland Parkway, was disciplined May 24 by the Taxation Department, which oversees the legal marijuana industry, said department spokeswoman Stephanie Klapstein. The department declined comment on the violations, saying only that Pisos was “out of compliance” with state regulations.
Multiple sources with knowledge of the suspension said Pisos failed a state-mandated inspection after authorities found unlabeled merchandise in its inventory. Dispensary representatives declined comment.
A Pisos representative said Wednesday just after 5 p.m. that the 24-hour dispensary was still closed but was prepared to open later in the evening.
Pisos first opened in June 2016 and is licensed to sell both medical and recreational marijuana.
At least four marijuana testing labs and one cultivation facility in Nevada have also had their licenses temporarily suspended.


One of rugby league’s all-round good guys Jarrod Croker will play his 300th NRL game on Friday when the Raiders host the Warriors at GIO Stadium.
Croker was rested last week to ensure he could celebrate the milestone on home soil.
Watch every game of every round of the 2023 NRL Telstra Premiership Season LIVE on Kayo Sports. New to Kayo? Start your free trial now >
It will be an emotional game for the 32-year-old, who has had to overcome several serious injuries to get to 300 games.
The Raiders are in red-hot form having won seven of their past eight games. They welcome back fullback Seb Kris from a knee injury and co-captain Elliott Whitehead from a two-game suspension too.
The Warriors bounced back from a loss last week when star halfback Shaun Johnson steered them to a 30-8 win over the Dolphins.
Coach Andrew Webster has been forced into making a change to his backline though with…


text:
Recycling reality show contestants normal
MasterChef champ Julie Goodwin was one of four recycled reality show contestants to enter the "jungle" in I'm A Celebrity Get Me Out Of Here!
She's been denied the rare opportunity of winning a second reality series after being ousted on Tuesday night while the first season Bachelor Tim Robards departed a few weeks ago.
However, Anna Heinrich, who was the last woman standing on The Bachelor, could still take out the title.
Or Chrissie Swan, who was the runner-up to Reggie Bird on season three of Big Brother, could go one better the second time around.
Here's some reality show contestants who have been recycled.
(Original reality show, year and placing)
Rob Mills (Australian Idol, 2003, 5th) - He has appeared on reality shows on all three commercial networks. Was 10th on Dancing With The Stars in 2009 and the fourth fired on The Celebrity Apprentice in 2013.
Fiona Falkiner (Biggest Loser, 2006 4th) - Tried to boogie her way to success but was the first eliminated, to finish 10th, on Dancing With The Stars in 2006.
Johnny Ruffo (X Factor, 2011 3rd) - He might not have won the karaoke competition but he strutted his way to success on Dancing With The Stars in 2012.
Sophia Pou (MKR, 2013 6th) - The MKR villain was recruited by Dancing With The Stars in 2013. She was the fourth contestant eliminated.
Layla Subritzky (Big Brother, 2012 Runner-up) - The Nine Network re-hired Subritzky for The Celebrity Apprentice in 2013 in which she finished seventh.
Nathan Joliffe (The Amazing Race, 2011 Won) - Almost pulled off a second reality show win when he was a worthy runner-up to Ian "Dicko" Dickson in The Celebrity Apprentice in 2012.
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domain: celebrity


Authors go to school at Deltona High
, Staff Writer
The Deltona High School Literacy Leadership Team is sponsoring "Authors' Night" from 6 to 8 p.m. Monday in the school's media center.
Several independent authors from around the country, who are members of the Paranormal Plumes Society, will visit Deltona High School to encourage students to become not only readers, but writers. Eight authors will be available to discuss how to make the dream of becoming a published author a reality.
The event will include a book signing and is open to the public. Deltona High is at 100 Wolf Pack Run, Deltona.
For information, call Mary Thomas, Deltona High reading coach, 386-575-4153.