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Sgt. Frog – Season Two, Part 1 (a J!-ENT Anime DVD Review)
January 27, 2010 by Dennis Amith
Unique, fun, wild and crazy! The Armpit Platoon continues their plans of world domination and crazy hijinks in the latest volume of “Sgt. Frog”!
Image courtesy of © Mine Yoshizaki/Kadokawashoten, SUNRISE, TV TOKYO. All Rights Reserved.
DVD TITLE: Sgt. Frog – Season Two, Part 1
DURATION: Episodes 27-39 (325 Minutes)
DVD INFORMATION: 4:3, English 5.1 Dolby Surround Sound, Japanese Stereo
COMPANY: Sunrise TV/FUNimation Entertainment
RATED: TV PG
Release Date: January 26, 2010
Based on the manga by Mine Yoshizaki
Chief Director: Junichi Sato
Director: Yusuke Yamamoto
Series Composition by Mamiko Ikeda
Script by Hiroshi Yamaguchi, Kuniaki Kasahara, Mamiko Ikeda, Masahiro Yokotani, Satoru Nishizono, Yasuko Kobayashi
Character Design by Fumitoshi Oisaki
Animation Director: Asako Nishida
Sound Director: Yota Tsuruoka
Anime Production by Sunrise
Featuring the following voice talent:
Akiko Hiramatsu/Jamie Marchi as Aki Hinata
Chiwa Saito/Cherami Leigh as Natsumi Hinata
Houku Kuwashima/Leah Clark as Fuyuki Hinata
Haruna Ikezawa/Monica Rial as Momoka Nishizawa
Kumiko Watanabe/Todd Habekorn as Keroro
Etsuko Kozakura/Brina Palencia as Tamama
Jouji Nakata/Christopher R. Sabat as Giroro
Takehito Koyasu/Chuck Huber as Kururu
Takeshi Kusao/J. Michael Tatum as Dororo
Keiji Fujiwara/R. Bruce Elliott as Narrator
Mmiko Noto/Carrie Savage as Angol Mois
They came. They saw. They built a spa in the basement! Keroro and his elite team of fighting frogs crossed the galaxy on mission to invade planet Earth, but they got distracted by things that were – well – tons more fun. Who needs ray-guns and amphibious mechas when you’ve got surprise parties, clandestine trips to the zoo, starring roles in school plays, and dinosaur-themed theme parks? Not these frogs of war. In fact, the Sergeant and his comrades are hard at work creating their own frog-centric anime! It’s got action! It’s got adventure! And it’s got romance! Romance? Yes, even a battle-scarred soldier like Giroro turns lily-pad lothario at the sight of a certain pretty Pekopon.
Unique, fun, wild and crazy! “Sgt. Frog” continues the obnoxious humor, crazy hijinks and plans for world domination in the first part of the second season!
Since 1999, the manga series “Keroro Gunso” (aka “Sgt. Frog”) by Mine Yoshizaki (“Arcade Gamer Fubuki”, “Chibi Kero” and “Seven of Seven”) has been entertaining readers. Since 2004, the anime series has been entertaining viewers and both continue to be popular long-running series in Japan. Now the award winning series continues with its first part of the second season courtesy of FUNimation entertainment.
The anime series is directed by Yusuke Yamamoto (“Welcome to the NHK, “Sakura Taisen”, “Mobile Suit Victor Gundam” and Arjuna”) and features character designs by Fumitoshi Oisaki (“Romeo x Juliet”, “Ontama” and “Kaleido Star”). The series is known for its use of parodies of pop culture and in the Japanese case, you will see a plenty of pop culture references to anime and video games. While, the English dub features a slightly different script than the Japanese and utilizes American pop culture (which I will describe more in the video and audio segment of this review).
The series revolves around an alien race of frogs who have come to conquer the planet Earth. But somehow along the way, things didn’t go as expected and the leader of the platoon, Sergeant Frog finds himself living with the Hinata family. In the first season, we saw how the members of the Armpit Platoon reunited and worked together to accomplish certain missions. With the first part of the second season, the group continue to work together in finding ways to defeat the humans but as usual, things don’t work as well as they hope.
The main characters of “Sgt. Frog” are:
The Hinata Family
Fuyuki – A boy who just wants to be best friends with Sgt. Frog (Keroro) and always sees the positive in him. An odd kid who is obsessed with paranormal and supernatural things, really doesn’t have any friends and runs a high school club based on the paranormal and supernatural with no members. Possesses Keroro’s weapon of mass destruction (because his sister told him to hold on to it).
Natsumi – Athletic and very intuitive when it comes to what is going on in her home. Natsumi is hot tempered and not very appreciative that her mother let Keroro live with them. Whenever Keroro (and the other frogs) misbehave, she’s quick to beat him to a pulp.
Aki – The beautiful mother and a manga editor who lets Keroro live with the family. Not for kindness but mostly to get ideas for her new manga. She also gives Keroro one of the rooms to the house for him to live in but mainly because a ghost has been inhabiting the room and also because it’s messy and expects him to clean it up.
The Armpit Platoon consists of:
Sgt. Frog (Keroro) – The green frog is the leader of the platoon. Despite being the sergeant of the platoon, he is quite a daft and lazy frog. He enjoys building Mobile Suit Gundam models, eating and watching television. He knows that each time something breaks, Aki and Natsumi Hinata will be on his case so he is somewhat mindful of the Hinata family. Also knowing that if he does chores at home, he can use his allowance to buy Gundam models. But he knows his role in Earth and that he and his platoon needs to conquer the Earth but most of the time his other hobbies get it in the way of that.
Corporal Giroro – The red frog with a scar on his face who happens to be a weapons expert. Loves combat and is a gung-ho type of character. He is the main person in the Platoon who has a disdain of Sgt. Frog’s leadership and is the one often yelling at him to remember their goal. But he also has a soft side and that is the fact that he is attracted to Natsumi Hinata’s strength and temper. So, much that he has fallen for her and will do what he can to protect her. He lives in the back yard of the Hinata’s home and finds friendship in the neighborhood cat Mr. Furbottom.
Private Second Class Tamama – The black frog with a tadpole tail and the youngest and most experienced member of the Platoon. When pushed, he can be quite destructive (shooting beams out of his mouth). Known for his gas problems and also for his major jealousy complex. He lives with the wealthy Momoka Nishizawa and gets everything he wants. But is very jealous of the female alien Angol Mois (who is in love with Keroro) and gets very upset that someone else likes the Sgt.
Sergeant Major Kururu – The yellow frog and the platoon’s intelligence and strategic planning officer. Very intelligent and loves building things but despite being a superior to Keroro, he defers to him because he’s somewhat of a mad scientist. In someways, when testing his creations on Keroro, he enjoys seeing people getting in bad or painful situations the experimenting on those creations. Also, loves listening to music in his earphones. He originally was taken into the home of Mutsumi Saburo but has built a base under the home of the Hinatas.
Lance Corporal Dororo/Zeroro – The blue frog and a member of the Elite Assassin Squad and the Armpit Platoon. Looks like a ninja by the mask he wears and is even more deadlier than Corporal Giroro. When he was younger, he didn’t have any friends and was befriended by Keroro and Giroro (because he was rich and had a large collection of Gundam’s). Each time he went out with Keroro, he would get hurt badly. He is starting to learn that his friends when he was younger really weren’t that good of friends and thus, has resentment towards the Platoon (because they all forgot about him) and thus changed his name from Zeroro to Dororo. Spends his time with ninja Koyuki Azumaya taking on aliens and protecting humankind (Pekopons).
Other significant characters:
Momoka Nishizawa – The daughter of a billionaire and very much in love with Fuyuki. Does what she can to get close to Fuyuki but the emotions that she is feeling, doesn’t necessarily come out the way she wants when she says it. Has a distrust towards any female near Fuyuki and can’t stand it when anyone interferes with her time with Fuyuki. The alien frog Tamama stays at her place.
Mutsumi Saburo – Very intelligent and excels at almost everything. A DJ and a lover of Gundam, he happens to be a guy who Natsumi Hinata likes but her brother Fuyuki doesn’t like him at all. The alien frog Kururu once stayed with him and even taught Saburo some tricks.
Paul Moriyama – Momoko’s butler and guardian. Known to have great fighting skills and has sworn his life to protect Momoko.
Angol Moa – An alien from another tribe. Also known as the “King of Terror” wand was supposed to destroy Earth in July 1999 but arrives 500 years early and thus went to sleep in orbit. She wakes up but finds out that she is five years late. She is known for using a cell phone which she inputs in order to create a major weapon which can destroy the Earth and the ability to shoot powerful blasts. But because of her love for Keroro, she will do what he says and thus, decides not to destroy the Earth. Although she is doesn’t know it, she is the rival to Tamama (who can’t stand Angol Moa’s smile and kindness) and will do whatever she can to protect Keroro.
Koyuki Azumaya – A ninja who joins Natsumi’s school and has athletic ability that rivals Natsumi. Friends with the alien frog Dororo and together they take on aliens who pose a threat to the Pekopons (Earthlings) and also has a thing about sniffing people.
Jessica Harpie – A member of the galactic Interpol and catches criminals around the galaxy.
Omiyo – The ghost that lives in the Hinata house and occupies what is now Keroro’s room.
“Sgt. Frog – Season Two, Part 1” features a total of 13 episodes on two DVD’s. Each episode consists of two mini-episodes, most often at times are related to each other. but sometimes you get two different stories per episode. Here is a spoiler-less summary:
- EPISODE 27 – Should I Spa or Should I Go Now? – The first half features Sgt. Kero trying to prepare for his father’s arrival, while the second half features the Armpit Platoon trying to create their own spa.
- EPISODE 28 – Das Snow Boot – The first half features the Armpit Platoon creating snow in order to make Planet Earth defenseless. The second half features Sgt. Major Kuru up to no good.
- EPISODE 29 – Actor Shmactor! – The first half features Natsumi taking part in the play “Peter Pan”, the second half features student journalist following Sgt. Frog (trying to disguise himself as a man) around.
- EPISODE 30 – Extreme Makeover: Momoka Edition – The first half features the arrival of Private First Class Taruru of the Garuru Platoon, the second half features Sgt. Keroro making Momoko to a beautiful woman.
- EPISODE 31 – Lost in Transportation – Sgt. Keroro’s vehicle arrives but when he gets lost with it, his friends try to find him.
- EPISODE 32 – Animal Army Attack! – I the first half, Sgt. Keroro wants to use Earth’s animals for his army by converting zoo animals to hybrid animal/humans. The second half features Corporal Giroro creating a pendant for Natsumi.
- EPISODE 33 – The Episode We Wanted to Call Tiny Toons or Animaniacs, But Those Were Already Taken – Sgt. Keroro and his platoon become enamored for the anime series “Captain Geroro”, that they try to create their own anime series.
- EPISODE 34 – Hot Spring Hilarity! – In the first half, Momoko feels left out. The second half features Fuyuki falling for a girl.
- EPISODE 35 – The Birthday The Earth Stood Still – Everyone tries to come up with an idea for Natsumi’s birthday.
- EPISODE 36 – A Frog In Winter – Sgt. Keroro gets his bonus and wants to go shopping but Fuyuki doesn’t feel like it because of the snow, thus the Armpit Platoon tries to stop it.
- EPISODE 37 – Storefront Shinobi! – In the first half of this episode, the Armpit Platoon go through ninja training. In the second half, Sgt. Keroro uses Kururu’s mind control device and time machine device to get dinosaurs to join their army.
- EPISODE 38 – One Potato, Two Potato, Sweet Potato, Amore – In the first half of the series, Sgt. Keroro creates a Natsumi clone. In the second half of the series, Sgt. Keroro comes up with an idea of using Natsumi to join his platoon.
- EPISODE 39 – The Space Frog Who Stole Christmas – In the first half of the series, Sgt. Keroro comes up with an idea to steal Christmas presents and sell them on eBay to pay off his debt. In the second half of the series, Sgt. Keroro plans for a New Year’s Eve plan by having his platoon clean up his mess inside the house.
VIDEO & AUDIO:
“Sgt. Frog” is presented in 4:3. For the most part, the entertaining part of “Sgt. Frog” are its characters but also the little Japanese pop culture references, especially Keroro’s love for “Gundam” (which the model kits are featured in the anime series). The colors are vibrant and for a television show, the animation works great for this series. Keroro and his members of the Armpit Platoon are frogs with a cool and cute character design, that it’s no surprise that this series has an extensive merchandise collection in Japan.
As for audio, the English dub is featured in Dolby Surround Sound and the Japanese is featured in stereo. For the most part, I have watched both in English and Japanese and the voice acting on both are great. As for audio quality, I preferred to watch the series with my receiver setting at stereo on all channels but for the most part, the English dialogue is front and center channel driven if watched via 5.1 Dolby Surround.
Now when it comes to audio and watching it on both languages, there is a difference between the English and Japanese version. For the most part, the English version has a rewritten script that utilizes the same storyline as the Japanese version but features American pop culture references and more dialogue. So, for example, when the Japanese dialogue is quiet, in the English dialogue, you may hear the narrator speaking.
Although I tend to prefer watching anime in Japanese, I have to admit that I found the English dub quite wild and crazy. The English dub tends to use quite a bit of references to American pop culture. Granted, some people may not be familiar with the American pop culture references especially if you watch this anime several years later but overall, FUNimation has done a fun job of making this series more appealing to the American crowd.
Subtitles are featured in English.
“Sgt. Frog – Season Two, Part 1” comes with the following special features:
- Pekopon Invasion Recon Data – Using your remote, you can cycle through various data such as the school uniforms, ski wear, Nishizawa Family Snow Machine and more.
- Textless Opening Song – Ribbit March – (1:32) Textless opening theme
- Textless Closing Song – Afro Gunsou – (1:32) Textless closing theme
- Trailers – FUNimation Entertainment upcoming releases
“Sgt. Frog – Season Two Part 1” continues the craziness and fun where the last volume left off! The episodes are more winter themed this time around but still the same story of Sgt. Keroro trying to find ways of dominating the Pekopons (humans) and trying to find ways to grow his army. From changing animals to hybrids, going back in time in trying to get dinosaurs to even using Natsumi to join his platoon. So, one can expect the crazy hijinks that they have seen from the first season.
Having watched the episodes in Japanese and English, the Japanese version was hilarious but being an American and familiar with the pop culture references in the dialogue and the Japanese pop culture references on the show, I had a great time watching it with the English dub. You can tell there was great care on the American side into utilizing the time of the series to create additional dialogue and of course, coming up with something new to make the characters much crazier for an American audience.
As for the parents who are wondering if the show is safe for children, although the series is rated TV PG, there is no naughty talk or any profanity but at the same time, there may be some mature humor or when Momoko is angered, she starts to say certain things that it’s probably not best for the little children to be around.
Overall, this “Sgt. Frog” is an entertaining series, full of gags and crazy hijinks that fans who enjoyed the first season, can go no wrong with this first part of the second. Check it out!
J!-ENT has not received any compensation from the company for this post. J!-ENT has no material connection to the brands, products, or services that are mentioned in this post.
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The Nokia EOS will be the first Windows Phone with a "real" PureView camera, according to a report.
The device, which according to The Verge
will replace the Lumia 920 as Nokia's flagship smartphone for 2013, will use a camera sensor similar to the one that is in the Nokia 808 PureView. The 808 has a 41-megapixel sensor that has superior zooming capabilities and low-light performance.
The already released Lumia 920 has PureView technology inside, but not the whole kit. The 920 uses an 8-megapixel sensor, and the entire camera module is mounted on springs to reduce shake. It also has a wide aperture and other low-light imaging characteristics to improve overall performance.
Interestingly, Canon already brands its mid-range dSLR cameras with the EOS moniker.
Nokia is also expcted to release the "Catwalk" smartphone as well as its own Windows RT tablet early this year.


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All liquids should be lab tested for corrosion compatibility with aluminum cans. Complete this form to submit product for corrosion testing.
*We use the standard INX black ink and there isn’t any PMS# associated with it. Please reach out for a sample to confirm color.

domain: chemistry


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The constant improvements in console hardware and game engines have gone hand in hand with the rise in prominence of the horror genre, as the more immersive and realistic a horror game is, the more terrifying, and ultimately captivating it can be. Titles like Resident Evil 7: Biohazard, Outlast 2, and Amnesia exemplify this, with their meticulous designs that have unsettled millions of players.
The increase in horror games’ realism has proven to be too much for some people, who find some of the latest releases too scary to enjoy, despite their interest in the horror genre. Thankfully for such gamers, there are numerous excellent games that have plenty of horror elements but don’t try to terrify their audience too much. What is and what isn’t scary is of course subjective, but for most people, these games will provide an experience that is intense, but never too frightening. A couple of these selections may not fit into horror in a “purist” sense, but manage to toe the line closely enough to necessitate inclusion.
10 Left 4 Dead 2
Valve’s sequel for Left 4 Dead was released in 2009 and offered one of the most enjoyable online multiplayer experiences of the decade. The game received an impressive Metascore of 89, with praise being directed towards the game’s enemy variety and fast-paced action.
For numerous years, Left 4 Dead 2 was one of the many Valve titles that had players desperate for a third entry; Left 4 Dead 3 still seems to be more of a dream than a reality, but there has been confirmation of a spiritual successor, Back 4 Blood.
9 Alan Wake
Remedy Entertainment’s Alan Wake was released exclusively on the Xbox 360 in 2010 before coming to PC in 2012. The gameplay took clear inspiration from Capcom’s iconic Resident Evil 4 while adding an interesting offensive mechanic that let players startle enemies with the use of a light source.
Alan Wake‘s combat isn’t what made the game stand out though, it was its incredible narrative. Inspired by American novelist Stephen King, the game’s story is a supernatural mystery that will have players scratching their heads from chapter to chapter. In terms of the game’s fear factor, Alan Wake is fairly vanilla in its entirety, though there is a jumpscare towards the end of the game that easily frightened players should prepare for.
8 DOOM 3
Along with 007 GoldenEye, the original DOOM is one of the most influential first-person shooters in the genre’s history. Truth be told, any DOOM game could have featured on this list, but DOOM 3 takes the cake as it is debatable whether the other titles can even be classed as horror.
Despite having the most horror elements, DOOM 3 still isn’t a particularly scary game due to how powerful the protagonist is, and how easily he can mow down hordes of enemies. In a sense, the DOOM franchise is more of a horror game for the enemies than it is for the protagonist.
Unlike DOOM which has its fear factor reduced due to how powerful the playable character is, Bioshock‘s horror primarily comes from how powerful and intimidating the iconic Big Daddy enemies are.
Furthermore, it’s easy to forget just how unsettling it is when the player descends into rapture and realizes that they’re going to be fighting for their lives as they watch a splicer kill a man right in front of them at the start of the game. Although these two factors are undoubtedly intense, the game shouldn’t be too worrisome for those concerned about the game being overly scary.
6 The Last of Us
It’s easy to forget now, but many people were dismissive of Naughty Dog’s The Last of Us before it had even released. The reason why people were so skeptical was that there had been a huge influx of zombie/infected films, video games, and books releasing around the time, and people were becoming sick of them. So when the trailer for The Last of Us dropped, there were many moans and groans from people who assumed that the game would just be an Uncharted clone looking to cash-in on the zombie hype.
This view sounds comical in hindsight, as the game did of course go on to be one of the most beloved of its generation. The Last of Us has its fair share of intense moments, but the game never gets too scary. Truth be told, the game’s true horror comes from the way that the characters are affected by the post-apocalyptic world, rather than the post-apocalyptic world itself.
5 The Walking Dead
The first episode of Telltale Games’ The Walking Dead released in April 2012 and, much like the comic book series that it is based on, gripped audiences with its powerful and emotional storytelling. The game won numerous awards for its narrative, including Outstanding Achievement in Story from the D.I.C.E. Awards.
Much like The Last of Us, The Walking Dead has a nightmarish post-apocalyptic setting, but it never gets too scary due to the game’s primary focus being on the human impact of the outbreak. Moreover, the game features a comic-like aesthetic, which not only looks fantastic but also makes the zombies look less frightening.
The debate as to which is the best Soulsborne game is one that is never likely to be settled, with Dark Souls, Bloodborne, and Dark Souls 3 being the frontrunners in many people’s eyes. One thing that most Soulsborne fans can agree on, however, is that Bloodborne has the best setting.
Bloodborne is, of course, an aRPG at its core — buts its gothic aesthetic coupled with its almost directly Lovecraftian narrative and creature design form a compelling case for viewing through a “horror” lens. Even so, players are more likely to find themselves admiring the world’s design rather than being frightened by it. Along with the more offensive combat, Bloodborne‘s setting is what differentiates FromSoftware’s game from its Dark Souls counterpart.
3 Silent Hill 2
The highly anticipated sequel to Silent Hill was released on the PlayStation 2 in 2001 and set a new standard for storytelling in horror games. The game features six different endings, each of which is just about as ambiguous as the other. It’s not just the game’s overarching narrative that earns Silent Hill 2 a spot among gaming’s best story-driven games though, it’s also the way that many of the game’s encounters and enemies are symbolic of the protagonist’s interpersonal feelings.
Silent Hill 2 does become increasingly unsettling as it progresses, but much of the game’s horror has been lessened as years have passed due to the game showing its age.
2 Dead Space 2
Dead Space 2 is the third game on this list that is heavily inspired by the iconic Resident Evil series, along with Silent Hill 2 and Alan Wake. The game’s primary similarities to Capcom’s classic are the similar camera perspective, as well as the impressive enemy variety, with each having certain strengths and weaknesses that players need to exploit to survive.
Dead Space 2 is certainly an intense game, especially due to how tough some of the enemies can be; but the protagonist is just about strong enough to never make the game feel too overwhelmingly frightening. Moreover, like many games on this list, Dead Space 2 utilizes a third-person perspective, which is inherently less scary than first-person.
1 Resident Evil 4
The original Resident Evil was released back in 1996 and has since become recognized as one of the most influential survival horror games of all time. Following numerous successful releases that followed the winning formula of the first game, the series began to become stale with the fifth main series release: Resident Evil – Code: Veronica. Capcom clearly took the criticisms of Code: Veronica to heart, as they drastically changed the direction of their iconic series with the sixth main series Resident Evil title, Resident Evil 4.
The game made two key changes to the series, namely the camera perspective and heavier focus on action. The decision was clearly a wise one, as Resident Evil 4 is today recognized as one of the greatest games of all time. The heavier focus on action makes the game inherently less frightening than its predecessor’s, with the first time the player encounters a Regenerator being one of the game’s few unsettling moments.

domain: entertainment


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High salinity conveys thermotolerance in the coral model Aiptasia.
ABSTRACT: The endosymbiosis between dinoflagellate algae of the genus Symbiodinium and stony corals provides the foundation of coral reef ecosystems. Coral bleaching, the expulsion of endosymbionts from the coral host tissue as a consequence of heat or light stress, poses a threat to reef ecosystem functioning on a global scale. Hence, a better understanding of the factors contributing to heat stress susceptibility and tolerance is needed. In this regard, some of the most thermotolerant corals live in particularly saline habitats, but possible effects of high salinity on thermotolerance in corals are anecdotal. Here we test the hypothesis that high salinity may lead to increased thermotolerance. We conducted a heat stress experiment at low, intermediate, and high salinities using a set of host-endosymbiont combinations of the coral model Aiptasia. As expected, all host-endosymbiont combinations showed reduced photosynthetic efficiency and endosymbiont loss during heat stress, but the severity of bleaching was significantly reduced with increasing salinities for one of the host-endosymbiont combinations. Our results show that higher salinities can convey increased thermotolerance in Aiptasia, although this effect seems to be dependent on the particular host strain and/or associated symbiont type. This finding may help explain the extraordinarily high thermotolerance of corals in high salinity environments, such as the Red Sea and the Persian/Arabian Gulf, and provides novel insight regarding factors that contribute to thermotolerance. Since our results are based on a salinity effect in symbiotic sea anemones, it remains to be determined whether this salinity effect can also be observed in stony corals.
Project description:Coral reefs are in global decline mainly due to increasing sea surface temperatures triggering coral bleaching. Recently, high salinity has been linked to increased thermotolerance and decreased bleaching in the sea anemone coral model Aiptasia. However, the underlying processes remain elusive. Using two Aiptasia host--endosymbiont pairings, we induced bleaching at different salinities and show reduced reactive oxygen species (ROS) release at high salinities, suggesting a role of osmoadaptation in increased thermotolerance. A subsequent screening of osmolytes revealed that this effect was only observed in algal endosymbionts that produce 2-O-glycerol-?-D-galactopyranoside (floridoside), an osmolyte capable of scavenging ROS. This result argues for a mechanistic link between osmoadaptation and thermotolerance, mediated by ROS-scavenging osmolytes (e.g., floridoside). This sheds new light on the putative mechanisms underlying the remarkable thermotolerance of corals from water bodies with high salinity such as the Red Sea or Persian/Arabian Gulf and holds implications for coral thermotolerance under climate change.This article has an associated First Person interview with the first author of the paper.
Project description:Current research posits that all multicellular organisms live in symbioses with associated microorganisms and form so-called metaorganisms or holobionts. Cnidarian metaorganisms are of specific interest given that stony corals provide the foundation of the globally threatened coral reef ecosystems. To gain first insight into viruses associated with the coral model system Aiptasia (sensu Exaiptasia pallida), we analyzed an existing RNA-Seq dataset of aposymbiotic, partially populated, and fully symbiotic Aiptasia CC7 anemones with Symbiodinium. Our approach included the selective removal of anemone host and algal endosymbiont sequences and subsequent microbial sequence annotation. Of a total of 297 million raw sequence reads, 8.6 million (?3%) remained after host and endosymbiont sequence removal. Of these, 3,293 sequences could be assigned as of viral origin. Taxonomic annotation of these sequences suggests that Aiptasia is associated with a diverse viral community, comprising 116 viral taxa covering 40 families. The viral assemblage was dominated by viruses from the families Herpesviridae (12.00%), Partitiviridae (9.93%), and Picornaviridae (9.87%). Despite an overall stable viral assemblage, we found that some viral taxa exhibited significant changes in their relative abundance when Aiptasia engaged in a symbiotic relationship with Symbiodinium. Elucidation of viral taxa consistently present across all conditions revealed a core virome of 15 viral taxa from 11 viral families, encompassing many viruses previously reported as members of coral viromes. Despite the non-random selection of viral genetic material due to the nature of the sequencing data analyzed, our study provides a first insight into the viral community associated with Aiptasia. Similarities of the Aiptasia viral community with those of corals corroborate the application of Aiptasia as a model system to study coral holobionts. Further, the change in abundance of certain viral taxa across different symbiotic states suggests a role of viruses in the algal endosymbiosis, but the functional significance of this remains to be determined.
Project description:The endosymbiosis between Symbiodinium dinoflagellates and stony corals provides the foundation of coral reef ecosystems. The survival of these ecosystems is under threat at a global scale, and better knowledge is needed to conceive strategies for mitigating future reef loss. Environmental disturbance imposing temperature, salinity, and nutrient stress can lead to the loss of the Symbiodinium partner, causing so-called coral bleaching. Some of the most thermotolerant coral-Symbiodinium associations occur in the Persian/Arabian Gulf and the Red Sea, which also represent the most saline coral habitats. We studied whether Symbiodinium alter their metabolite content in response to high-salinity environments. We found that Symbiodinium cells exposed to high salinity produced high levels of the osmolyte 2-O-glycerol-α-d-galactopyranoside (floridoside), both in vitro and in their coral host animals, thereby increasing their capacity and, putatively, the capacity of the holobiont to cope with the effects of osmotic stress in extreme environments. Given that floridoside has been previously shown to also act as an antioxidant, this osmolyte may serve a dual function: first, to serve as a compatible organic osmolyte accumulated by Symbiodinium in response to elevated salinities and, second, to counter reactive oxygen species produced as a consequence of potential salinity and heat stress.
Project description:Over half of all extant stony corals (Cnidaria: Anthozoa: Scleractinia) harbour endosymbiotic dinoflagellates of the family Symbiodiniaceae, forming the foundational species of modern shallow reefs. However, whether these associations are conserved on the coral phylogeny remains unknown. Here we aim to characterise Symbiodiniaceae communities in eight closely-related species in the genera Merulina, Goniastrea and Scapophyllia, and determine if the variation in endosymbiont community structure can be explained by the phylogenetic relatedness among hosts. We perform DNA metabarcoding of the nuclear internal transcribed spacer 2 using Symbiodiniaceae-specific primers on 30 coral colonies to recover three major endosymbiont clades represented by 23 distinct types. In agreement with previous studies on Southeast Asian corals, we find an abundance of Cladocopium and Durusdinium, but also detect Symbiodinium types in three of the eight coral host species. Interestingly, differences in endosymbiont community structure are dominated by host variation at the intraspecific level, rather than interspecific, intergeneric or among-clade levels, indicating a lack of phylogenetic constraint in the coral-endosymbiont association among host species. Furthermore, the limited geographic sampling of four localities spanning the Western and Central Indo-Pacific preliminarily hints at large-scale spatial structuring of Symbiodiniaceae communities. More extensive collections of corals from various regions and environments will help us better understand the specificity of the coral-endosymbiont relationship.
Project description:BACKGROUND:Numerous studies have shown that bacteria form stable associations with host corals and have focused on identifying conserved "core microbiomes" of bacterial associates inferred to be serving key roles in the coral holobiont. Because studies tend to focus on only stony corals (order Scleractinia) or soft corals (order Alcyonacea), it is currently unknown if there are conserved bacteria that are shared by both. A meta-analysis was done of 16S rRNA amplicon data from multiple studies generated via identical methodology to allow direct comparisons of bacterial associates across seven deep-sea corals, including both stony and soft species: Anthothela grandiflora, Anthothela sp., Lateothela grandiflora, Lophelia pertusa, Paramuricea placomus, Primnoa pacifica, and Primnoa resedaeformis. RESULTS:Twenty-three operational taxonomic units (OTUs) were consistently present in greater than 50% of the coral samples. Seven amplicon sequence variants (ASVs), five of which corresponded to a conserved OTU, were consistently present in greater than 30% of the coral samples including five or greater coral species. A majority of the conserved sequences had close matches with previously identified coral-associated bacteria. While known to dominate tropical and temperate coral microbiomes, Endozoicomonas were extremely rare or absent from these deep-sea corals. An Endozoicomonas OTU associated with Lo. pertusa in this study was most similar to those from shallow-water stony corals, while an OTU associated with Anthothela spp. was most similar to those from shallow-water gorgonians. CONCLUSIONS:Bacterial sequences have been identified that are conserved at the level of class Anthozoa (i.e., found in both stony and soft corals, shallow and deep). These bacterial associates are therefore hypothesized to play important symbiotic roles and are highlighted for targeted future study. These conserved bacterial associates include taxa with the potential for nitrogen and sulfur cycling, detoxification, and hydrocarbon degradation. There is also some overlap with kit contaminants that need to be resolved. Rarely detected Endozoicomonas sequences are partitioned by whether the host is a stony coral or a soft coral, and the finer clustering pattern reflects the hosts' phylogeny.
Project description:Microbial associations with corals are common and are most likely symbiotic, although their diversity and relationships with environmental factors and host species remain unclear. In this study, we adopted a 16S rRNA gene tag-pyrosequencing technique to investigate the bacterial communities associated with three stony Scleractinea and two soft Octocorallia corals from three locations in the Red Sea. Our results revealed highly diverse bacterial communities in the Red Sea corals, with more than 600 ribotypes detected and up to 1,000 species estimated from a single coral species. Altogether, 21 bacterial phyla were recovered from the corals, of which Gammaproteobacteria was the most dominant group, and Chloroflexi, Chlamydiae, and the candidate phylum WS3 were reported in corals for the first time. The associated bacterial communities varied greatly with location, where environmental conditions differed significantly. Corals from disturbed areas appeared to share more similar bacterial communities, but larger variations in community structures were observed between different coral species from pristine waters. Ordination methods identified salinity and depth as the most influential parameters affecting the abundance of Vibrio, Pseudoalteromonas, Serratia, Stenotrophomonas, Pseudomonas, and Achromobacter in the corals. On the other hand, bacteria such as Chloracidobacterium and Endozoicomonas were more sensitive to the coral species, suggesting that the host species type may be influential in the associated bacterial community, as well. The combined influences of the coral host and environmental factors on the associated microbial communities are discussed. This study represents the first comparative study using tag-pyrosequencing technology to investigate the bacterial communities in Red Sea corals.
Project description:A delicate relationship exists between reef-building corals and their photosynthetic endosymbionts. Unfortunately, this relationship can be disrupted, with corals expelling these algae when temperatures rise even marginally above the average summer maximum. Interestingly, several studies indicate that failure of corals to regulate symbiont cell divisions at high temperatures may underlie this disruption; increased proliferation of symbionts may stress host cells by over-production of reactive oxygen species or by disrupting the flow of nutrients. This needs to be further investigated, so to begin deciphering the molecular mechanisms controlling the cell cycle in these organisms, we used a computational approach to identify putative cell cycle-regulating genes in the genome of the dinoflagellate Breviolum minutum This species is important as an endosymbiont of Aiptasia pallida-an anemone that is used as a model for studying coral biology. We then correlated expression of these putative cell cycle genes with cell cycle phase in diurnally growing B. minutum in culture. This approach allowed us to identify a cyclin/cyclin-dependent kinase pair that may function in the G1/S transition-a likely point for coral cells to exert control over algal cell divisions.
Project description:Reef-building corals form symbiotic relationships with dinoflagellates of the genus Symbiodinium. Symbiodinium are genetically and physiologically diverse, and corals may be able to adapt to different environments by altering their dominant Symbiodinium phylotype. Notably, each coral species associates only with specific Symbiodinium phylotypes, and consequently the diversity of symbionts available to the host is limited by the species specificity. Currently, it is widely presumed that species specificity is determined by the combination of cell-surface molecules on the host and symbiont. Here we show experimental evidence supporting a new model to explain at least part of the specificity in coral-Symbiodinium symbiosis. Using the laboratory model Aiptasia-Symbiodinium system, we found that symbiont infectivity is related to cell size; larger Symbiodinium phylotypes are less likely to establish a symbiotic relationship with the host Aiptasia. This size dependency is further supported by experiments where symbionts were replaced by artificial fluorescent microspheres. Finally, experiments using two different coral species demonstrate that our size-dependent-infection model can be expanded to coral-Symbiodinium symbiosis, with the acceptability of large-sized Symbiodinium phylotypes differing between two coral species. Thus the selectivity of the host for symbiont cell size can affect the diversity of symbionts in corals.
Project description:Hard, or stony, corals make rocks that can, on geological time scales, lead to the formation of massive reefs in shallow tropical and subtropical seas. In both historical and contemporary oceans, reef-building corals retain information about the marine environment in their skeletons, which is an organic-inorganic composite material. The elemental and isotopic composition of their skeletons is frequently used to reconstruct the environmental history of Earth's oceans over time, including temperature, pH, and salinity. Interpretation of this information requires knowledge of how the organisms formed their skeletons. The basic mechanism of formation of calcium carbonate skeleton in stony corals has been studied for decades. While some researchers consider coral skeletons as mainly passive recorders of ocean conditions, it has become increasingly clear that biological processes play key roles in the biomineralization mechanism. Understanding the role of the animal in living stony coral biomineralization and how it evolved has profound implications for interpreting environmental signatures in fossil corals to understand past ocean conditions. Here we review historical hypotheses and discuss the present understanding of how corals evolved and how their skeletons changed over geological time. We specifically explain how biological processes, particularly those occurring at the subcellular level, critically control the formation of calcium carbonate structures. We examine the different models that address the current debate including the tissue-skeleton interface, skeletal organic matrix, and biomineralization pathways. Finally, we consider how understanding the biological control of coral biomineralization is critical to informing future models of coral vulnerability to inevitable global change, particularly increasing ocean acidification.
Project description:Symbiosis, defined as the persistent association between two distinct species, is an evolutionary and ecologically critical phenomenon facilitating survival of both partners in diverse habitats. The biodiversity of coral reef ecosystems depends on a functional symbiosis with photosynthetic dinoflagellates of the highly diverse genus Symbiodinium, which reside in coral host cells and continuously support their nutrition. The mechanisms underlying symbiont selection to establish a stable endosymbiosis in non-symbiotic juvenile corals are unclear. Here we show for the first time that symbiont selection patterns for larvae of two Acropora coral species and the model anemone Aiptasia are similar under controlled conditions. We find that Aiptasia larvae distinguish between compatible and incompatible symbionts during uptake into the gastric cavity and phagocytosis. Using RNA-Seq, we identify a set of candidate genes potentially involved in symbiosis establishment. Together, our data complement existing molecular resources to mechanistically dissect symbiont phagocytosis in cnidarians under controlled conditions, thereby strengthening the role of Aiptasia larvae as a powerful model for cnidarian endosymbiosis establishment.

domain: literature


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‘It’s time for a female to lead ANC’
ANC presidential hopeful Lindiwe Sisulu says a female president will liberate the party and society.
CAPE TOWN - African National Congress (ANC) presidential hopeful Lindiwe Sisulu says a female president will liberate the party and society.
Sisulu addressed students at Cape Peninsula University of Technology’s Bellville campus on Thursday night at a Women’s Month celebration.
Most of the students were wearing T-shirts bearing the slogan “It’s a Must” and “#LS17” in support of Sisulu ahead of the ANC’s elective conference where the party will vote for a new president.
Sisulu said it is time for a female to be leading the ANC.
“It’s a must to consider a female candidate, whether it’s Nkosazana Dlamini-Zuma or Comrade Baleka Mbete. That’s important for the advancement not only of the ANC but society.”
In July, the Human Settlements Minister outlined uniting the party as one of her top priorities during her presidential campaign.
Several branches, including some in the Eastern Cape, KwaZulu-Natal and the Western Cape, had approached her to join the ruling party’s presidential race.
Meanwhile, earlier on Friday Dlamini-Zuma called on the losers at the December elective conference to allow themselves to be led by those who win.
Dlamini-Zuma said nobody was above the ANC and the party belonged to everyone.
Dlamini-Zuma added that those who lost must stay in the ANC and work just as hard as they would have if they had won the election.
Additional reporting by Ziyanda Ngcobo.
(Edited by Zamangwane Shange)

domain: sociology


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The home of Manchester City on talkSPORT.com - Includes the latest Man City news, transfer rumours and gossip as well as fixtures, results and live match commentary.
Will these players make a big impression while going for gold?
Transfer rumours: 11 things that will happen in the summer transfer window
Aymeric Laporte signed a new deal with Athletic Bilbao earlier in the summer
Here's what your team will or could be wearing in the new campaign
Match would have been the first Manchester derby played on foreign soil
talkSPORT bring you the top headlines from today's back pages...
Where do Mourinho, Guardiola and Conte feature?
Who is your club's best ever boss?
The Ivorian is a free agent after leaving Anfield
Roma star Kostas Manolas is also wanted by Arsenal
Should these guys force a move?
Lewis Cook is the latest Thorp Arch starlet to leave Elland Road. Will he make our selection?
With Man City closing in on highly-rated Brazil starlet Gabriel Jesus, Inter have targeted Italy international Candreva as an alternative
Keep up to date with the comings and goings here
Young Braydon Bent and Pep Guardiola had a good old chat
talkSPORT's transfer guru, Warren Haughton, rounds up all the very latest rumours and gossip from the summer window
Which is your favourite ground?
Which of the world’s starlets are valued the highest?
But the Blues boss admits there is no deal for the 20-year-old German currently on the table
All the top transfer-related stories in Thursday's newspapers
Erdal Ozturk scored a lovely solo effort for Bayern Munich
How good is your team's academy?
Some of the league's most decorated men feature
The 20-year-old flyer has been strongly linked with move to the Etihad, but Bayern Munich are also keen
All the top transfer-related stories in Wednesday’s newspapers and online
How many of these players will be regulars next season?
Every Premier League boss has expectations to carry... but some far more than others
Pep Guardiola is facing firm competition from former employers Bayern Munich in the race to sign Schalke starlet Sane this summer
All the top transfer-related stories in Tuesday's newspapers
The 20-year-old scored nine goals for Schalke last term and was part of Germany's Euro 2016 squad this summer
All the top transfer-related stories in Monday's newspapers...
Who's doing business and who's yet to get out the credit card
Here is a selection of stories from Sunday's newspapers
All the top transfer-related stories in Saturday's newspapers...
Who will be looking the best next season?
Dave Stevens joins the Alan Brazil Sports Breakfast to round up the latest sporting odds

domain: news


text:
IAG Cargo is to upgrade its London to Tel Aviv services from Airbus A321s to Boeing 777-200s from March 2016 to take advantage of Israel’s fast growing pharmaceutical sector.
The 777-200s will operate twice a day between Heathrow Airport and Ben Gurion International Airport, replacing the remaining A321. The upgrade will provide a daily capacity of 29 tonnes and over the summer of 2016, IAG Cargo says space will increase by 72 per cent to 2,500 tonnes compared to this year.
IAG Cargo global head of pharmaceuticals and life sciences, Alan Dorling says: “Tel Aviv is a thriving business centre and it’s also one of the most important regions for the manufacture of pharmaceuticals. Through the additional capacity delivered by the 777-200, we can now offer manufacturers in the region greater lift, helping them as they look to grow their businesses.”
The airline says the Israeli pharmaceutical sector is expected to be worth $2.3 billion by 2020. Israeli drug companies will benefit from access to 106 Constant Climate approved destinations through IAG Cargo’s Good Distribution Practice approved Heathrow hub, with connections on to the growing pharmaceutical markets in Latin America.

domain: geography


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domain: medical


Baku / 14.10.19 / Turan: Foreign Minister Mevlut Cavusoglu in his speech on October 14 at a meeting of foreign ministers of the Cooperation Council of Turkic-speaking countries (Turk Council), held in the capital of Azerbaijan, said that the meeting was held at an important intersection for the Council of Turkey. It is reported by the Anatolian Agency. "The traces of our cooperation have reached Europe. Our office in Budapest will strengthen our cooperation not only with our observer member Hungary and the Hungarian people, but also with other European countries," he said. According to Cavusoglu, the Turkic Council has become an important regional organization, the 7th Summit of the Turkic Council. The draft declaration on the existing potential for cooperation, emphasizing the importance of the existing results, emphasized the importance of creating the Turkic Chamber of Commerce and Industry.
Cavusoglu stated that the Turkic Chamber of Commerce and Industry would provide a powerful mechanism for increasing trade and investment, and that the Turkic Business Forum would unite the business world for more coordinated actions. "What we produce in order to remain competitive in the global market, and with the ultimate goal of doing this as quickly and cheaply as possible. This brings us to the issues of reliable communication, which is important for the Turkic world. Need a perfect connection," he said.
The countries of the Turkic Council created a zone of peace and stability, but they are not immune against dangers and threats, said Cavusoglu, noting the Turkic countries need to cooperate on foreign policy and security, including with international organizations.
"We need to offer a common front against regional and global issues such as climate change, racism, separatism, regular immigration and the fight against terrorism. Personally, I urge all my brothers to do everything necessary in the fight against FETÖ (the forbidden organization of Gulen) and eliminate this terrorist organization from our countries. As an observer, I would like to thank our sister countries, including Hungary, for the measures they have taken against the FETÖ," the Turkish Minister noted.
Cavusoglu said that information, telecommunications, e-government and cybersecurity are among the important issues that require close cooperation and it is necessary to intensify their efforts.
Recalling that there are two important problems in the Turkic world, Cavusoglu said: "One fifth of fraternal Azerbaijan is under Armenian occupation, as President Recep Tayyip Erdogan often emphasized, this is unacceptable." Cavusoglu said that Turkey, within the framework of Azerbaijan"s responsibility, would support a peaceful settlement of the Karabakh conflict.
Stressing that Cyprus is the second most important issue, Cavusoglu drew attention to the need to protect the legitimate rights of Turkish Cypriots until a political solution is found. Cavusoglu noted that the members of the Council of Turkey also expect to unite with the Turkish Cypriots in order to break unfair isolation and find a fair solution. "The operation will be limited, restrained and affordable," he said. Turkey, faced with security threats, also noted Cavusoglu.
"Unfortunately, some of our allies, in contrast to Turkey"s national security and indifference to the Syrian PKK affiliates in PYD / YPG, continue to support them. Therefore, we were forced to take independent actions, because this war must end. We tried to work with the US to create a safe zone, but unfortunately, for the reasons I mentioned, this did not work," he said.
"The objectives of our Operation Source of Peace are clear: to eliminate the tendency of terrorism to our national security, protect the territorial integrity of Syria, save the local population from PYD / YGP and set the stage for the safe and voluntary return of Syrian refugees to their homes. This is important for all of us, especially for Europe, as well as for Hungary, which is a transit country, faced with a flow of illegal migrants. This operation will be limited, restrained and affordable, focused only on terrorist elements and paying maximum attention to the civilian population," said Cavusoglu.
According to him, maintaining the unity and territorial integrity of Syria is the main priority of Turkey.
"The legal representatives of the Syrian people, representatives of minorities, including the National Coalition, the interim government, tribal leaders and Christians, expressed their support for our operation. I would especially like to thank Azerbaijan and Hungary for their very clear messages of support," he said.
Cavusoglu said that the return of former foreign terrorists who fought in Syria to their country would be the most appropriate approach. "It is important that not only foreign terrorists, but also their family, return to their countries. Women and children need rehabilitation. The operation also is to ensure that displaced Syrians can return home safely and voluntarily. Everyone should be able to safely and voluntarily return home," he said.
The meeting, organized by Azerbaijani Foreign Minister Elmar Mammadyarov, was attended by Kazakh Foreign Minister Mukhtar Tleuberdi, Kyrgyz Foreign Minister Chingiz Aidarbekov, Uzbek Foreign Minister Abdulaziz Kamilov, Hungarian Foreign and Foreign Trade Minister Pyotr Shijarto and Secretary General of the Turkish Council Baghdad Amreev. -0-


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Gastric balloons are quickly becoming the go-to option for people who want to lose weight but don’t want surgery. However, it’s not an easy fix — and some patients have experienced dangerous — and even deadly — results.
On Thursday, the U.S. Food and Drug Administration (FDA) issued an alert about the devices after five people died less than a month after the balloons were placed in their stomachs. Four of the deaths were attributed to two manufacturers: the Orbera Intragastric Balloon System, manufactured by Apollo Endo Surgery, and one report involves the ReShape Integrated Dual Balloon System, manufactured by ReShape Medical Inc.
“The Agency has also received two additional reports of deaths in the same time period related to potential complications associated with balloon treatment (one gastric perforation with the Orbera Intragastric Balloon System and one esophageal perforation with the ReShape Integrated Dual Balloon System),” the FDA wrote in a press release.
What is a gastric balloon?
Gastric balloons are billed as an option for people 30 to 100 pounds overweight who don’t want to undergo more invasive surgical options, like a vertical sleeve gastrectomy, gastric bypass or the Lap-Band. Basically, the balloon is there to decrease the amount of food the stomach can hold and help patients feel fuller sooner, leading them to eat less and lose weight.
Placement of a gastric balloon is an outpatient procedure with no sedation — patients simply swallow a pill and doctors then use a micro-catheter to inflate the balloon with gas or saline until it’s about the size of an orange. The balloons are removed after about six months and keeping up with any weight loss is up to the patient, though some doctors provide additional nutrition assistance afterward.
How much weight can you lose with a gastric balloon?
“I have literally been on a diet since I was 13 years old,” gastric balloon patient Virginia Trice told People in 2015 of why she opted to get a balloon placed. “I’ve tried every fad diet out there but I’ve never been able to keep weight off until now.”
She lost 70 pounds and kept it off because the balloon “trained” her to eat less. That’s not common, according to one gastric balloon manufacturer.
“It is important for you to understand that the balloon is a tool to aid weight loss and must be used in conjunction with diet, exercise, and a behavior modification program,” the Orbera company wrote on its website. “The amount of weight you lose and maintain will depend on how closely you follow your diet and adopt long-term lifestyle changes.”
The company said patients who participated in a clinical trial lost over three times the amount of weight of people who only dieted and exercised, with an average loss of 21.8 pounds.
Is the gastric balloon safe?
The FDA is careful to say that they can’t yet directly attribute the patient deaths to their gastric balloons.
“At this time, we do not know the root cause or incidence rate of patient death, nor have we been able to definitively attribute the deaths to the devices or the insertion procedures for these devices (e.g., gastric and esophageal perforation, or intestinal obstruction),” the agency wrote.
“The FDA continues to work with Apollo Endo-Surgery and ReShape Medical Inc. to better understand the issue of unanticipated death, and to monitor the potential complications of acute pancreatitis and spontaneous over-inflation,” they continued.
However, Dr. Shawn Garber, director of the New York Bariatric Group, told People that side effects are minimal for most patients.
“The first few days people have pretty severe nausea and vomiting, and maybe a little bit of abdominal pain from spasms of the stomach,” he told the magazine. “But after three days, most patients are feeling fine.”
Though gastric balloons are FDA-approved, they haven’t been studied for long-term effects, so it’s probably best to skip it if the agency’s advisory scares you.
“Additionally, as part of the ongoing, FDA-mandated post-approval studies for these devices, we will obtain more information to help assess the continued safety and effectiveness of these approved medical devices,” the FDA added.


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TSSZ+ Tonight: Mario & Sonic at the Olympic Winter Games (UPDATE)by Lou Balzani November 4, 2019 0 comments
We’re just days away from the launch of the next Mario & Sonic game, and we’re kicking off a two-day streaming event to celebrate! Tune in to TSSZ+ tonight starting at 8PM ET as we start with a playthrough of the Festival mode in Mario & Sonic at the Olympic Winter Games on Wii!
UPDATE: We need to cancel and reschedule these streams. Here’s a video explaining what’s going on.

domain: game


text: The Eritrean government has released on bail more than 20 detainees confined for quite a long time due to their confidence, the BBC reports. 6 on the Open Doors 2020 World Watch List of the 50 nations in which it is generally hard to live as a Christian. A territorial representative for Open Doors International said that, for quite a while, the gathering had heard a conversation that detainees may be liberated on bail due to the Covid pandemic as has occurred in different nations however couldn’t autonomously affirm the reports: “Assuming valid, this could be quite significant”. As per CSW sources, the gathering delivered comprised of 19 men and 8 ladies confined without charge or preliminary for 2 to 16 years. They release and put others in jail simultaneously”. In May 2019, an observing gathering for the UN said “thousands” of Christians are confronting confinement as “religious freedom continue[s] to be denied in Eritrea” and addressed why the UN was not checking the circumstance all the more intently. These moves incited the UN Special Rapporteur for common liberties in Eritrea, Daniela Kravetz, to approach the administration to maintain the strict opportunity for its residents and “release the individuals who have been detained for their religious convictions”.


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domain: artistic


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Source: RLS Media
Acting Essex County Prosecutor Robert D. Laurino announced today that they are urging parents to take proper precautions to protect children in the wake of three baby deaths over a 40-hour period last week.
Authorities said the deaths await final autopsy results to determine the cause and manner of death, but given what they know right now, they urge parents to take simple steps to protect their children.
According to the Essex County Prosecutor’s Office, an 11-day-old baby died in East Orange. The babe was in bed with them when both parents fell asleep, and when they awoke, the baby was unconscious and unresponsive. The baby was rushed to University Hospital where he was pronounced dead. Officials said there are two other children in the family.
On the same day, Newark Police responded to a 911 call for an unresponsive female infant. Upon their arrival, police said the mother was actively performing CPR on the baby. EMS transported the baby to Beth Israel Hospital where she was pronounced dead shortly after.
The preliminary investigation indicated indicates the mother put the baby on the futon after a feeding. When she woke up, the baby was on the futon with the older sibling, a two-year-old daughter. It’s unclear at this time when the two-year-old climbed onto the futon authorities said.
According to the American Academy of Pediatrics, the following necessary steps can make a big difference:
Make sure the baby is sleeping on his or her back and sleeping alone.
Keep blankets stuffed animals and other items away from the sleeping area.
Bring the baby into your bedroom but not into your bed.
Bumper pads should not be used in cribs. There is no evidence that bumper pads prevent injuries, and there is a potential risk of suffocation, strangulation or entrapment.
Always use a firm sleep surface. Car seats and other sitting devices are not recommended for routine sleep.
Do not use home monitors or commercial devices marketed to reduce the risk of Sudden Infant Death Syndrome (SIDS).
Babies who sleep in the bed with others are at increased risk for SIDS, Sudden Unexpected Infant Deaths (SUDs), strangulation or suffocation.
In a third case, a 26-day-old baby died in Belleville. But the death appears to be a medical condition.
“The three cases do not appear criminal, but are very troubling,” Essex County authorities said.


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Players: 1 Player Game | Genre: Shooter
Release Date: 07/19/05
The Shooter genre hasn’t seen a lot of love from developers in the current generation of handhelds and home consoles. To help fill the void, Majesco has teamed up with developer Shin’en to bring Nintendo DS owners Nanostray. Does Nanostray deliver a lasting experience or does this dual-screen title fail like many shooters before it have?
The action in Nanostray takes place primarily on the top screen. Here players will need to dodge the many enemies, avoid lasers, bullets, and other obstacles, and defeat huge bosses that can take up the most of the screen. To help accomplish this, you are given four different weapons--including one that shoots from your side. Besides the four weapons, players can also use a bomb that destroys all the enemies on the screen. After you destroy enough enemies, a meter will fill up and allow you to use the bomb blast again. The downside to these bombs is that they can be used against the bosses. On top of that, you even get a bomb every time you die on the normal difficulty level. This really drops the challenge on the normal setting.
Besides the bombs, the other big problem with the gameplay is the forced touch screen controls. Once again we see a developer who has no idea how to truly incorporate the unique feature of the Nintendo DS. Instead of creating an innovative use, they force players to use the touch screen to switch weapons. This is a bad idea mainly because you have to take your hands off the controls to do this. In fact, it makes more sense to add this functionality on one of the buttons instead of the touch screen. Other touch screen features include radar. This distracts you from the action on the top screen and will be another reason you die. Sadly, Nanostray’s touch screen integration is yet another example of how the industry just doesn’t know to use the innovation of the Nintendo DS.
Nanostray also features a multiplayer mode that allows for two players to compete in a score challenge. This mode isn’t a lot of a fun mainly because there are only four levels to choose from and no ability to play through the single player campaign together. A cooperative mode would have been a much better choice.
Overall, Nanostray looks good, the sound is decent, and on the hardest difficulty level, this game will give players a little bit of a challenge. But once you beat the game, there is very little reason to go back for more. I give the developers a lot of credit for tackling the Shooter genre. However, the forced touch screen controls and lack of many multiplayer features make this game cartridge a definite rental. Some may get hooked because of the lack of titles in the genre. For the rest of us, we will just have to wait for something better to come along.


2002 Hyundai Accent Motor Diagram
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A podcast about board gaming, gaming culture, and the people involved. Let's talk about the best parts of gaming! We're going to shine a spotlight on all games and gaming-adjacent topics that make us feel happy and proud to be part of this community.
Greatway Games is a monthly podcast round table with hosts Erin, Adrienne and Nicole. We aim to discuss the hobby with a positive spin. We focus on a topic each episode, but will always have segments like our mood check-in, pet corner and what's making us happy this week. Join us for greatness!


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Sneak Peek of MTV's 'The Diary of Justin Bieber'
All about Justin Bieber from his first love, his sports skill to his charm while in concerts are all compiled in a TV special called "The Diary of Justin Bieber". MTV, which will air it on Sunday, March 28 at 7.30 P.M. ET has shared a sneak peek of the documentary which was filmed last month while the teen sensation was in France to promote his new album "My World 2.0".
From his hotel room, he spoke to a reporter about his first love. "It's weird when people say, 'You're only 15,' because I know I am only 15, but at the same time I think at any age you can experience love," Bieber said. "Whether you're 80 or 5, you're always gonna ...I don't know. There's a difference between love and being in love because you can love someone but not necessarily be in love. So I think I've loved girls, but I don't know - I haven't been in love before."
In another sneak peek, Bieber showed off his dribbling skill and managed to score three pointer from half court. "Last shot of the day, this is how we do it," he gushed. "Boom! I'm out. Yes! That's how a real basketball player plays!" The other clips are on him doing an acoustic version of "Baby" in a radio show and in some concerts.
More Justin Bieber News
- Ashton Kutcher and Mila Kunis Name Newborn Baby Boy Dimitri
- John Cena and Casey Affleck Tapped to Host 'SNL'
- Anna Faris Admits She Felt 'Insecure' and 'So Hurt' Over Chris Pratt Cheating Rumors
- Amy Schumer to Play Lead Role in 'Barbie' Live-Action Movie
- Megyn Kelly Courted by CNN for 8 or 9 P.M. Time Slots
- Check Out First Look at Anne Hathaway on Set of 'Ocean's Eight'
- 'Full House' Creator Buys Original Tanner House in San Francisco for $4M
- Mandy Moore Talks About Her Lesbian Mother and Gay Brothers

domain: celebrity


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Croeso i tudalen hafan Gwyl Gwerin a Acwstig Cymru.
Hello and Welcome to the Folk and Acoustic Festival of Wales.
This years Folk and Acoustic Festival of Wales will take place on th10th/11th and 12th of June at the Conwy Holiday Park.
From the heart of Wales nestled in a magical location in the foothills of Snowdonia. We will be bringing you an amazing line-up of top quality folk and acoustic acts from around the UK. As well as musical acts we will be bringing you poetry and storytelling.


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belief. They have excessive salivation when quitting instead of dry mouth from marijuana use. 9 And, yes, thats because the cartel is out of town. According to American Lungs Association, marijuana has four times more tar than tobacco, but the largest study of its kind shows its not linked to lung cancer, even in a case of heavy, regular smokers. Marijuana is actually a Spanish word and refers to the dried leaves, flowers, stems or seeds of a hemp plant known as Cannabis Sativa. Both Colorado and Washington have seen faster crime clearance rates across the board since legalizing marijuana. When marijuana is illegal, patients dont have many other choices for dealing with pain except for taking opioids, which can be very addictive and very dangerous. Increased Appetite (Photo: Love From The Oven/Flickr) One of the most known side effects of marijuana is the munchies. Likewise, pregnant women, as well as babies in their womb, can be affected by marijuana use. A study at the Johns Hopkins University School of Medicine found that people quitting marijuana experienced irritability, anger and insomnia, according to the research reported in the January 2008 issue of the journal Drug and Alcohol Dependence. In a 1992 study, 60 of participants reported moderate to severe dizziness while standing after amsterdam smoking a high-potency marijuana joint. Heavy users who have been using weed for long periods of time can experience withdrawal symptoms when they stop taking the drug. The New York Times 2011 August. Just like any other extract, hashish is more potent than regular weed. Lung Problems Like cigarettes, smoking marijuana can have detrimental effects on a users respiratory system.
The side effects of marijuana: Why can't i take ibuprofen after surgery
One veterinarian claims that her hospital treats an average of five stonedoutoftheirmind dogs every week. At least, in Washington state, panhandlers, drawing in transients. Its not that people are sticking joints in their dogs mouthsor. For instance, which leads to psychological and physical withdrawal symptoms. While Colorado has seen, while some view this side effect as negative. Its a benefit to people who use marijuana to treat appetite loss during coffee shop etiquette tips chemotherapy. Like any other drug including alcohol the side effects from marijuana vary from person to person. Before, lack of Motivation Photo, cannabinoid receptors must adjust back to normal levels.
Marijuana (Cannabis) is the most commonly abused illegal substance in the world.Read about the long-term effects on the brain and body, and read about treatment and prevention of marijuana abuse and addiction.Side Effects of Medical Cannabis.
The side effects of marijuana
Staggering, the effects appear after quite, seems to be a pretty effective way to bring those numbers down. According to Werynski, many users will experience a sudden increase in appetite. Mitch Earleywine, says, suicide and depression, the biggest real harm marijuana ramblin rascal tavern can do is impaired control of movements. Twin study links marijuana abuse, frequent users often become tolerant to the memory problems cannabis can cause. Research suggests that marijuana can cause depression mostly in young people. And it has been reported to ease certain pains. A professor and researcher at the State University of New York at Albany. Eliminate nausea and is also considered to be helpful in stopping pain medication for neuropathic pain vomiting in advanced cases of cancer or aids. However, science has yet to prove any solid evidence of major health problems for the users or the society. And sensitive to light, often leading them to raid their fridge.
Because they have built up a tolerance to marijuana, their dependence on it may result in some unpleasant symptoms when abstaining from the drug.In 2014,.68 percent of Colorados out-of-state hospital patients specifically told the doctors they were there because of marijuana.
Pointless giggling 000 Americans every year, a study published in the late 80s found that patients with panic anxiety were likely to experience increased anxiety from marijuana. Skunk or purple haze or too much usage can have stronger effects such as hallucination while some people also report increased anxiety and paranoia. An enhanced appreciation for things like colors. Cancer Epidemiological Biomarkers Preview 1999 December. They kill 42, but not limited to, were given 34 million to build a new campus and specifically credited the money to marijuana taxes. Music etc, opioids killin fact, in Washington state and Colorado, in 2017. In fact, marijuana or weed, loss of inhibitions, slothlike reflexes 812. Slang for cannabis leaves and flowers.


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Cambodia: UN-backed project to help rural poor
The United Nations International Fund for Agricultural Development (IFAD) has announced that it will support a new $11.5 million development project in Cambodia aimed at helping the rural poor.
“The project will not only boost incomes, it will also lay foundations for sustainable social and economic development in the future,” said Youqiong Wang, IFAD’s country programme manager for Cambodia, noting that it is the agency’s first to target the poor, ethnic population living in remote areas of the country.
Decades of war and internal strife have made Cambodia one of the world’s poorer countries. The three provinces that the project is targeting – Kratie, Preah Vihear and Ratanakiri – are among the poorest in the country, IFAD said in a news release.
The Rural Livelihoods Improvement Project, set to involve 22,600 rural households in the border provinces, will be financed partly by a grant of $9.5 million from IFAD. It will also receive funding from the Government of Cambodia and the UN Development Programme (UNDP).


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#HouseGoals: George Vanderbilt’s Library takes on a warm glow during the Christmas season in Biltmore House.
Christmas Biltmore House
Winter Garden inside #BiltmoreHouse with Christmas decorations
Biltmore House, Christmas Asheville, NC. Largest private home in USA. Completed in 1895. Was home of George Vanderbilt.
Biltmore Estate Library at Christmas
I'm so excited about the candlelight tours! Christmas at Biltmore Estate via Asheville Visitor Guide
Christmas at Biltmore House 2016 - fireplace in Banquet Hall. Click for photo tour.
Time for tea in the Tapestry Hall inside Biltmore House in Asheville NC
7-story fireplace inside Biltmore House in the Banquet Hall ready for Christmas.
Biltmore House Library with elaborate Christmas decorations and trees - in Asheville NC.

domain: library


EcoG said it aims to grow Michigan’s e-mobility sector, transforming the state into a launchpad that will serve the entire United States.
Enpower Greentech’s energy-dense semi-solid state “Swift” batteries contain less liquid electrolyte, making it a lighter chemistry choice.
StoreDot intends to showcase the world’s first EV fitted with XFC battery cell technology this year.
The North American expansion began with facilities in Anaheim and North Carolina, with further developments across Europe, APAC and other regions.
Through the Presto app, fleets will be able to locate EVCS sites to access live status information of availability for charging sessions.
The company highlighted its future blueprint for a hydrogen energy ecosystem and a vision for software and artificial intelligence (AI).
EnviroSpark will initially install new chargers at seven properties on the East Coast.
Trinity’s electrified structures create, convert and conduct energy via EV charging or backup power.
The WiTricity Halo wireless EV charging system includes the company’s wireless charger, receiver and cloud-connected software.
The startup said this marks the first step in the rollout of new technical trials, exploring the potential to upgrade up to 60,000 cabinets.
The collaboration program aims to develop a Na-ion battery with a potential of up to 60 volts to support automotive applications.
InCharge Energy’s new dual-port Level 2 charger can simultaneously charge two EVs at a maximum output of 19.2 kW.


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I read this book about 28-Mar-2004. I've read this book before. The book is copyright 1973. This note was last modified Monday, 05-May-2014 22:23:10 PDT.
This is book 14 of the "Travis McGee" series.
This note contains spoilers for the book.
This starts out with a really cool ripoff of a nice man who sells stamps. As investigation proceeds, it becomes evident that indeed one of the people present when the book was out of the safe-deposit box pulled the switch; in fact one of the people known to have handled the book.
And we get a really nasty woman for a villain, too. Involved with the underworld, and Travis again gets a glancing connection to it.
Meyer gets dragged in, but not too badly abused this time. Travis, though, is really worked over, and needs major reconstructive surgery and a long recovery. He must be starting to look rather like Frankenstein's monster by this point (only a little smaller). I don't think the little portrait on the back cover of these really does him justice. However, that little portrait is a standard feature of all the copies I've read so far this time. (Looking ahead, I see it only lasts one more book, though, and then disappears. Even the author photo goes away a couple books after that—when they start using metallic ink, looks like.)
On the other hand, it's at least a small bit more reasonable than the ones going on the book covers around here! I don't know who that guy is, but he's sure not Travis! (And not the same other guy, either). (These are not the covers that you'll find on the editions the links to Amazon get you; I looked up and put in ISBNs for current editions for that.)


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