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Fall is arguably the best time of year to read a book. Mornings and nights are starting to get chilly, which is perfect for curling up with a book and a cup of tea. Meanwhile, days are still warm enough to sit outside and read while the leaves are falling around you.
So whether you’ve been eating pumpkin spice flavored foods since August, or you haven’t packed away your beach towel just yet, there’s one great reason to look forward to autumn. Fall is a fantastic time for new book releases!
Here are some of the most highly-anticipated fiction releases for fall 2017!
Let’s start with some heavy hitters. These are releases from well-known authors with loyal followings. Fans eagerly anticipate their next releases, so these fiction titles are guaranteed to be popular the moment they hit the shelves!
Sleeping Beauties by Stephen King and Owen King
Stephen King is immensely popular, but this new release by King has a fun twist: it was co-authored with his son Owen! You might already know Stephen’s other son, Joe Hill, who has enjoyed his share of success (and has a new book, Strange Weather, coming out in October).
In a near-future where women succumb to a sleeping disease and men revert to their primal natures, one mysteriously immune woman struggles to survive in an Appalachian town where she is treated as both a demon and a lab specimen.
Artemis by Andy Weir
Don’t recognize the author’s name? Weir wrote The Martian, which was a huge hit. Both the book and the movie were very popular here at the library. Weir’s new novel, set on the moon, promises to be equally intriguing.
Jazz Bashara is a criminal. Well, sort of. Life on Artemis, the first and only city on the moon, is tough if you're not a rich tourist or an eccentric billionaire. So smuggling in the occasional harmless bit of contraband barely counts, right? Everything changes when Jazz sees the chance to commit the perfect crime and learns that she's stepped square into a conspiracy for control of Artemis itself.
Origin by Dan Brown
When Dan Brown writes a new book, we get ready to order a lot of copies. Brown, who enjoyed success with The Da Vinci Code, continues his series of action-packed mysteries featuring Robert Langdon!
Robert Langdon arrives at the ultramodern Guggenheim Museum Bilbao to attend a major announcement--the unveiling of a discovery by one of Langdon's first students at Harvard that "will change the face of science forever."
These prize-winning authors have won praise from critics and readers alike. Accomplished authors, they aren’t afraid to take their fiction in surprising new directions.
Fresh Complaint: stories by Jeffrey Eugenides
Eugenides impressed readers and critics with his fascinating Pulitzer Prize-winning book, Middlesex. It’s been years since he last published a book, so Fresh Complaint, his first collection of short stories, promises to be popular.
A first collection of short stories by the Pulitzer Prize winner includes the tales of a failed poet-turned-embezzeler, a young traveler seeking enlightenment, and a high schooler whose drastic decision upends a British physicist's life.
Manhattan Beach by Jennifer Egan
Egan won the 2011 Pulitzer Prize for A Visit from the Goon Squad, a story about an aging punk-rocker and a young woman with a compulsion to steal. Egan’s new release is a lyrical, character-driven novel with a historical background.
Years after she is placed in the hands of a stranger vital to her family's survival, Anna takes a job at the Brooklyn Naval Yard during the war while meeting with the man who helped them and learning important truths about her father's disappearance.
The Golden House by Salman Rushdie
Multiple Booker award-winner Rushdie has written a book that covers big issues in current times: media, culture, and politics. The narrator of the book is a filmmaker who gains the trust of the aristocratic Golden family, documenting their triumphs and downfalls.
A thriller inspired by today's headlines follows the experiences of a real-estate tycoon and his mysterious, corrupt family, who become the subjects of an aspiring filmmaker's project before revelations of monstrous past activities give way to the rise of a mad presidential candidate.
Successful debut authors working on subsequent books often admit that their writing processes have changed. After success, there’s understandably a lot of pressure and deadlines! Because they’ve had a lifetime of storing up great ideas, sometimes debut authors really nail their first books!
George & Lizzie by Nancy Pearl
Nancy Pearl is also known as “America’s Librarian,” so librarians worldwide are rooting for her debut! After a lifetime of reading and reviewing books, she’s written a book of her own!
The daughter of two renowned narcissistic psychologists, Lizzie’s problem has always been overthinking everything. George, raised in a very adoring family, comes into Lizzie’s life with one goal – to love her completely and forever. Can she relinquish the past to move toward the happiness that could be hers in the future?
The Floating World by C. Morgan Babst
Another debut that promises to be excellent, The Floating World is set in New Orleans during and after Hurricane Katrina. It’s particularly relevant in the wake of hurricanes Harvey and Irma.
Refusing to evacuate when Hurricane Katrina approaches the Louisiana Coast, Cora, the fragile eldest daughter of the Boisdores family, inadvertently triggers a chain of events that strains her parents' marriage and subjects her to a mysterious act of violence.
The Blind by A. F. Brady
Psychological thrillers are enjoying their time in the spotlight, and this one promises to be pretty twisted!
Sam James has spent years carefully crafting her reputation as the best psychologist at Typhlos, Manhattan's most challenging psychiatric institution. She believes if she can't save herself, she'll save someone else. It's this savior complex that serves her well in helping patients battle their inner demons, though it leads Sam down some dark paths and opens her eyes to her own mental turmoil.
These are topping my TBR (To Be Read) list! If you’ve read and loved previous books by each of these authors, there’s a good chance you’ll enjoy these new releases too!
Little Fires Everywhere by Celeste Ng
Celeste Ng’s debut, Everything I Never Told You, won multiple awards and was named Best Book of the Year in 2015 by more than fifteen major publications. Ng is adept at exploring family dynamics and social drama, making her novels both fascinating and relevant.
In Shaker Heights, a placid, progressive suburb of Cleveland, everything is planned - from the layout of the winding roads, to the colors of the houses and the successful lives its residents will go on to lead. No one embodies this spirit more than Elena Richardson, whose guiding principle is playing by the rules. When Mia Warren arrives in this idyllic bubble with her teenaged daughter Pearl, her mysterious past and a disregard for the status quo threatens to upend this carefully ordered community.
Miss Kopp’s Midnight Confessions by Amy Stewart
In the third book in the Kopp Sisters novels, we return to the tough and clever Constance Kopp, based on a true story of a female deputy sheriff in 1916 Hackensack, N.J.
Deputy Sheriff Constance Kopp and her sister Fleurette defend the young women being brought into the 1916 Hackensack jail under dubious charges like waywardness and incorrigibility.
Sing, Unburied, Sing by Jesmyn Ward
Salvage the Bones, which won a National Book award, was such a beautifully written book, while covering such heavy topics as teen pregnancy, dog fighting, poverty, and Hurricane Katrina. Ward’s new book follows in a similar vein of heavy topics described in gorgeous prose.
Living with his grandparents and toddler sister on a Gulf Coast farm, Jojo navigates the challenges of his tormented mother's addictions and his grandmother's terminal cancer before the release of his father from prison prompts a road trip of danger and hope.
Love and Other Consolation Prizes by Jamie Ford
Ford couldn’t have picked a more accurate title for the popular Hotel on the Corner of Bitter and Sweet, which had everyone smiling and crying in equal measures. I imagine that Ford’s new novel will elicit similar reactions, as we explore another disturbing episode in history. But don’t worry—amidst tragedy, Ford will yet again offer a bit of hope.
For 12-year-old Ernest Young the chance to go to the World's Fair feels like a gift. But only once he's there, amid the exotic exhibits, fireworks, and Ferris wheels, does he discover that he is the one who is actually the prize. The half-Chinese orphan is astounded to learn he will be raffled off--a healthy boy "to a good home."

domain: library


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Poker is a game that requires high levels of thinking and reasoning skills. It is a game that requires you to evaluate the odds and make decisions under uncertainty. This is a skill that is useful in many other areas of life, including business and investing. This game also teaches you to deal with failure and loss. The most successful players learn to accept losses as part of the game and they never try to chase their losses. This allows them to continue to improve their game and build up a large bankroll.
The first step in learning to play poker is understanding the rules of the game. This involves studying the charts of what hands beat what and the different ways that you can win a hand. It also includes knowing the basic betting rules. For example, in poker you can “call” a bet or you can raise it. If you call, you must match the amount of money that was previously bet by your opponent. You can also say “raise” if you want to add more money to the pot than your opponent did.
Another important part of learning to play poker is evaluating the risk in each hand. This means that you must know what your chances are of winning and losing and how much you can afford to lose. You must also be able to read your opponents and pick up on their tells. This is done by analyzing their betting behavior and looking for patterns in how they move around the table.
There is no point in trying to beat the other players at the table if you can’t read them. It is important to mix up your style of play so that your opponents are not sure what you have in your hand. If they always know what you have, you will not get paid off when you have strong value hands and your bluffs will not succeed.
Lastly, you must learn how to play your strong value hands as straightforwardly as possible. This will help you to outplay your opponents and to capitalize on their mistakes. It is common for amateurs to slowplay their strong value hands in order to try to outwit their opponents, but this strategy often backfires and ends up costing them more money than they would have won if they had just played the hand straight.
Ultimately, the best way to learn to play poker is to practice it as much as you can. This will give you the most experience and allow you to make the best decisions under uncertainty. However, it is also important to keep in mind that poker can be a mentally demanding game and it is not something that you should play when you are tired or emotionally upset. If you are feeling frustrated or fatigued, quit the session right away. You’ll be happier and more likely to perform at your best tomorrow.

domain: hobby


MetaNet is a software project initiated by ErikWalthinsen as an attempt to make building network services easier. At a high level it is a message-passing infrastructure designed to allow programs to tap into each others' control flow. Services such as sockets and HTTP servers are objects with their own set of mailboxes (in the form /daemon/object/message), some of which are hooked by the object itself, some of which are intended for their users to hook.
A good example of the reason for this architecture is the case of DHCP and LDAP. Start with a DHCP server implemented on MetaNet. It will create a listening socket on the right port, and attach a function to catch incoming packets. Those are converted to a DHCP request message, which normally is hooked by a function that does the normal DHCP lookup in the free-pool. That function sends a message to the DHCP-reply mailbox, which triggers a function to send the right packet.
Say you had an LDAP database that held the mapping from hostname/mac to IP, and you wanted to drive DHCP with this. You'd write a function that would hook the DHCP-request message and instead create an LDAP request. A second function would take the LDAP response and reformat it to the DHCP reply, to be handled again by the DHCP code to convert it to a packet and send it to the machine.
Captive Portal Feature Requests
- A simple blog/guestbook so people can leave comments and the node owner can post messages. Perhaps loading in portions of the splash page into a text box (psuedo wiki style) would be a good way to allow node owners to do updates and customize their page with relevant information.
- Actually a better way to do this is to point it at the NodeXXX wiki page for comments. It should be able to retrieve it's node ID number from the mapping software or possibly just provide a text box which will just append to a wiki page for them to make it easier.
Another more decentralized way to do this would be to store comment data within MetaNet and then to write a wiki module for (eg. [[NodeData(351)]]) which pulls basic information from the MappingSoftware and then also pulls comment data from the MetaNet instance itself. If it's not up you probably don't care about the comments
- Some way for ICQ/Jabber address to be listed on the status page so people can find each other.
A UrlDaemon implementation. It could feed status page information and perhaps have a web front end to allow the owner to update the broadcasts.
Should be able to update the MappingSoftware with a date stampe everytime it's used. Feedback of this sort is the absolute best monitoring we can get.
Map maintenance front end. The owner of a node should be able to put in all their information into the admin interface of MetaNet, and MetaNet should be able to publish (or send updates) to the map server. An advantage of this is that the node then has information like latitude and longitude which an AdhocRouting protocol could potentially take advantage of.
It should keep track of locally (and ideally post to the MappingSoftware) statistics on node use. Number of times used per day/week/month/year, amount of bandwidth used, number of visitors etc. This allows people to have a sense of ownership and accomplishment from running their node. For example, I have no idea if anyone ever uses my node ... thus I tend to be very casual about fixing it when it breaks. If I know that it was getting regularly used then I would be much more willing to put in some effort to keeping it running.
So this is getting a little disorganized. I've been thinking a lot about how to make MetaNet interoperate with the maps server and how to make the whole thing as decentralized as possible. It would be cool if all node maintenance could be done via MetaNet (which acts as a map server client). When you click on the map software to few a nodes details it could be served straigh from the web server in MetaNet (proof that it's up).
When first created it should be able to create or download a config from the MappingSoftware.
I'd like to be able to offer my wireless machine services not available to the public wireless users of my node. For example, I'd like my wireless laptop to see my shared drives on my home's server, but I don't want public users of my node to be able to use these services. DavidSmith


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Buford, GA Restaurants
The Dawg House
4965 Lanier Islands Pkwy Buford, GA 30518 (770) 932-9940
THE DAWG HOUSE is the alternative quick serve eatery that provides a wide variety of fresh, hot, delicious quality food at a very resonable price. Starting with the build it your own way burger and dawg concept with over 25 toppings to the award winning buffalo wings, some of the best southern fried fish & shrimp on the east coast, to our potato fries with the skin on and our chef's unique blend of fusion seasoned fried chicken, philly cheese Steaks, po' boys and much much more.
THE DAWG HOUSE Is simply a great place to grab a delicious bite to eat.
Tannery Row Ale House
554 W Main St Buford, GA 30518 (678) 765-8979
We are the home of the best barbecue, pizza, sandwiches and libations on this side of the tracks! Located in the Historic Tannery Row building, our menu features modern comfort food inspired by the history & culture of our surroundings. Be sure to check out our Event Calendar as we offer live music, poker tournaments, specials and more. We can’t wait to see you at the Ale House!
Bare Bones Steakhouse
115 E Main St Buford, GA 30518 (470) 266-1890
Bare Bones is a classic Steakhouse. We are located in the Bona Allen building in downtown Buford, GA. You will experience the feel of the old saddlery and harness making shops that once occupied this space. We have a traditional restaurant and bar and also offer a comfortable lounge and additional space for parties and events.
5 Brothers Pizza & Pasta
39 E Main St NE Buford, GA 30518 (678) 765-8151
Growing up in an Italian home is full of excitement ! Who's yelling over who , who is trying to stuff food down your throat until you can't move or who is trying to love you more ! We never knew what to expect ! As I got older and spent more and more time in the kitchen learning how to cook , I always felt it in my heart I would bring my families recipes to life and let the world get to experience them !
The mouth watering taste of Italian food from the big city of New York, finally lands in the south !!! This is based on family recipes passed on from generation to generation ! Your dinning experience will feel like a typical Italian Sunday dinner , a lot of food mixed with the warm and loving atmosphere !!! What's better then going out to a restaurant with your loved ones and feeling like you are in the comfort of your own home !
So here I am now with my husband and five sons , waiting for you to re-live these experiences with us !!
As my grandma Lucy would say "
Venire sedersi e mangiare " -
Come sit down and eat !
West Main Trade
123 W Main St Buford, GA 30518 (678) 765-6826
Antique and Consignment with a Gourmet Coffee Shop located in a 5,000 square foot renovated historic theater in downtown Buford. West Main Trade is now open to serve our neighbors by offering consignment merchandise and gourmet coffee. Come visit our historic building and shop our 25 spaces and enjoy your favorite gourmet coffee or tea beverage.
Reserved for Sperata
Reserved for Adams Restaurant

domain: food


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Thank you for reading the news about Dr. Rimah Saleh Al-Yahya, deputy minister of private higher education and now with the details
Jeddah - Yasmine El Tohamy - RIYADH: The Public Prosecution has launched, in collaboration with the Absher platform, an electronic service that allows citizens and residents to submit complaints in case of any violation of the rights of detainees arrested for breaking the law.
Sheikh Abdullah bin Nasser Al-Muqbel, the department’s assistant undersecretary for prison supervision and enforcement of sentences, said the new service reflects keenness to supervise prisons, ensure justice and preserve citizens’ rights.
He added: “The Public Prosecutor will follow up the case, go to where the detainee is held, conduct the necessary investigations, order the detainee’s release if there are irregularities in his arrest, and take necessary measures against perpetrators of the illegal arrest.”
Al-Muqbel said the Public Prosecution is developing its monitoring role by increasing transparency.
In 2017, King Salman issued a decree to amend the name of the Bureau of Investigation and Prosecution to the Public Prosecution, and linking its office directly to the king. Minister of Justice Walid Al-Samaani said linking the Public Prosecution directly to the king is an important step which comes in line with measures practiced in countries with developed judiciaries, and in accordance with Islamic Shariah.
These were the details of the news Dr. Rimah Saleh Al-Yahya, deputy minister of private higher education for this day. We hope that we have succeeded by giving you the full details and information. To follow all our news, you can subscribe to the alerts system or to one of our different systems to provide you with all that is new.
It is also worth noting that the original news has been published and is available at Arab News and the editorial team at AlKhaleej Today has confirmed it and it has been modified, and it may have been completely transferred or quoted from it and you can read and follow this news from its main source.

domain: news


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1 TRI Reagent For processing tissues, cells cultured in monolayer or cell pellets Catalog Number T9424 Store at room temperature. TECHNICAL BULLETIN Product Description TRI Reagent is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA, and protein. Successful isolations from human, animal, plant, yeast, bacterial, and viral samples can be obtained. A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA, and protein. 1 This procedure is an improvement of the single-step method reported by Chomczynski and Sacchi 2 for total RNA isolation. TRI Reagent performs well with large or small amounts of tissue or cells and many samples can be simultaneously extracted. This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein on homogenization or lysis of tissue sample. After adding chloroform or 1-bromo- 3-chloropropane and centrifuging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. One ml of TRI Reagent is sufficient to isolate RNA, DNA, and protein from mg of tissue, cells, or 10 cm 2 of culture dish surface for cells grown in monolayer. This is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh tissue or cells. The procedure is very effective for isolating RNA molecules of all types from kb in length. The resulting RNA is intact with little or no contaminating DNA and protein. This RNA can be used for Northern blots, mrna isolation, in vitro translation, RNase protection assay, cloning and polymerase chain reaction (PCR). The DNA is in the interphase and phenol phase, which forms after the addition of chloroform or 1-bromo- 3-chloropropane to the TRI Reagent in Sample Preparation, step 2. After precipitation and multiple washes, the DNA is dissolved in 8 mm NaOH. The solution is neutralized and the DNA is ready for analysis. The resulting DNA is suitable for PCR, restriction enzyme digestion, and Southern blotting. After precipitating the DNA with ethanol (DNA Isolation, step 1), the proteins can be removed from the phenolethanol supernatant. The isolated material can be probed for specific proteins by Western blotting. 1 Reagents Required but Not Provided RNA Isolation: Chloroform, Catalog Number C2432, or 1-Bromo-3-chloropropane, Catalog Number B Propanol, Catalog Number I % Ethanol 1 mm sodium phosphate, Catalog Number S3264, ph 8.2, 0.5% SDS solution, Catalog Number L4522, diluted 20-fold, formamide, or diethylpyrocarbonate-treated water DNA Isolation: 8 mm NaOH 0.1 M trisodium citrate, 10% ethanol solution Absolute ethanol 75% ethanol EDTA Protein Isolation: 2-Propanol, Catalog Number I9516 Absolute ethanol, Catalog Number E % Ethanol, Catalog Number E7148 1% SDS, Catalog Number L4522, diluted 10-fold 0.3 M Guanidine hydrochloride, Catalog Number G3272, in 95% ethanol
2 2 Precautions and Disclaimer This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices. Storage/Stability Store the product at room temperature. Procedures Sample Preparation 1A. Tissue: Homogenize tissue samples in TRI Reagent (1 ml per mg of tissue) in a Polytron or other appropriate homogenizer. Note: If minimal shearing of the DNA is desired, use a loosely fitting homogenizer, not a Polytron (see DNA Isolation, step 3, note b). The volume of the tissue should not exceed 10% of the volume of the TRI Reagent. 1B. Monolayer cells: Lyse cells directly on the culture dish. Use 1 ml of the TRI Reagent per 10 cm 2 of glass culture plate surface area. After addition of the reagent, the cell lysate should be passed several times through a pipette to form a homogenous lysate. Note: TRI Reagent is not compatible with plastic culture plates. 1C. Suspension cells: Isolate cells by centrifugation and then lyse in TRI Reagent by repeated pipetting. One ml of the reagent is sufficient to lyse animal, plant, or yeast cells, or 10 7 bacterial cells. a. If samples have a high content of fat, protein, polysaccharides, or extracellular material such as muscle, fat tissue, and tuberous parts of plants an additional step may be needed. After homogenization, centrifuge the homogenate at 12,000 g for 10 minutes at 2-8 C to remove the insoluble material (extracellular membranes, polysaccharides, and high molecular mass DNA). The supernatant contains RNA and protein. If the sample had a high fat content, there will be a layer of fatty material on the surface of the aqueous phase that should be removed. Transfer the clear supernatant to a fresh tube and proceed with step 2. Recover the high molecular mass DNA from the pellet by following DNA Isolation, steps 2 and 3. b. Some yeast and bacterial cells may require a homogenizer. c. After the cells have been homogenized or lysed in TRI Reagent, samples can be stored at 70 C for up to 1 month. 2. Phase Separation: To ensure complete dissociation of nucleoprotein complexes, allow samples to stand for 5 minutes at room temperature. Add 0.1 ml of 1-bromo-3-chloropropane or 0.2 ml of chloroform (see Phase Separation, notes a and b) per ml of TRI Reagent used. Cover the sample tightly, shake vigorously for 15 seconds, and allow to stand for 2 15 minutes at room temperature. Centrifuge the resulting mixture at 12,000 g for 15 minutes at 2-8 C. Centrifugation separates the mixture into 3 phases: a red organic phase (containing protein), an interphase (containing DNA), and a colorless upper aqueous phase (containing RNA). a. 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA. 4 b. The chloroform used for phase separation should not contain isoamyl alcohol or other additives. c. For isolation of poly A + fraction from the aqueous phase see Appendix I. RNA Isolation 1. Transfer the aqueous phase to a fresh tube and add 0.5 ml of 2-propanol per ml of TRI Reagent used in Sample Preparation, step 1 and mix. Allow the sample to stand for 5 10 minutes at room temperature. Centrifuge at 12,000 g for 10 minutes at 2-8 C. The RNA precipitate will form a pellet on the side and bottom of the tube. Note: Store the interphase and organic phase at 2-8 C for subsequent isolation of the DNA and proteins. 2. Remove the supernatant and wash the RNA pellet by adding a minimun of 1 ml of 75% ethanol per 1 ml of TRI Reagent used in Sample Preparation, step 1. Vortex the sample and then centrifuge at 7,500 g for 5 minutes at 2-8 C. a. If the RNA pellets float, perform the wash in 75% ethanol at 12,000 g. b. Samples can be stored in ethanol at 2-8 C for at least 1 week and up to 1 year at 20 C.
3 3 3. Briefly dry the RNA pellet for 5 10 minutes by airdrying or under a vacuum. Do not let the RNA pellet dry completely, as this will greatly decrease its solubility. Do not dry the RNA pellet by centrifugation under vacuum (Speed-Vac ). Add an appropriate volume of formamide, water, or a 0.5% SDS solution to the RNA pellet. To facilitate dissolution, mix by repeated pipetting with a micropipette at C for minutes. a. Final preparation of RNA is free of DNA and proteins. It should have a A 260 /A 280 ratio of 1.7. b. Typical yields from tissues (µg RNA/mg tissue): liver, spleen, 6-10 µg; kidney, 3-4 µg; skeletal muscle, brain, µg; placenta, 1-4 µg. c. Typical yields from cultured cells (µg RNA/10 6 cells): epithelial cells, 8-15 µg; fibroblasts, 5-7 µg. d. Ethidium bromide staining of RNA in agarose gels visualizes two predominant bands of small (2 kb) and large (5 kb) ribosomal RNA, low molecular mass ( kb) RNA, and discrete bands of high molecular mass (7 15 kb) RNA. DNA Isolation 1. Carefully remove the remaining aqueous phase overlaying the interphase and discard. To precipitate the DNA from the interphase and organic phase, add 0.3 ml of 100% ethanol per 1 ml of TRI Reagent used in Sample Preparation, step 1. Mix by inversion and allow to stand for 2 3 minutes at room temperature. Centrifuge at 2,000 g for 5 minutes at 2-8 C. Note: Removal of the remaining aqueous phase before DNA precipitation is a critical step for the quality of the isolated DNA. 2. Remove the supernatant and save at 2-8 C for protein isolation. Wash the DNA pellet twice in 0.1 M trisodium citrate, 10% ethanol solution. Use 1 ml of wash solution for every 1 ml of TRI Reagent used in Sample Preparation, step 1. During each wash, allow the DNA pellet to stand (with occasional mixing) for at least 30 minutes. Centrifuge at 2,000 g for 5 minutes at 2-8 C. Resuspend the DNA pellet in 75% ethanol (1.5 2 ml for each ml TRI Reagent) and allow to stand for minutes at room temperature. a. Important: Do not to reduce the time samples remain in the washing solution. Thirty minutes is the absolute minimum time for efficient removal of phenol from the DNA. b. If pellet contains >200 µg of DNA or large amounts of non-dna material, an additional wash in 0.1 M trisodium citrate, 10% ethanol solution is required. c. Samples suspended in 75% ethanol can be stored at 2-8 C for several months. 3. Dry the DNA pellet for 5 10 minutes under a vacuum and dissolve in 8 mm NaOH with repeated slow pipetting with a micropipette. Add sufficient 8 mm NaOH for a final DNA concentration of µg/µl (typically ml to the DNA isolated from mg of tissue or 10 7 cells). This mild alkaline solution assures complete dissolution of the DNA pellet. Centrifuge at 12,000 g for 10 minutes to remove any insoluble material and transfer the supernatant to a new tube. a. A viscous supernatant indicates the presence of high molecular mass DNA. b. The size of the DNA will depend on the force exerted during homogenization. Avoid using a Polytron homogenizer. c. Samples dissolved in 8 mm NaOH can be stored at 2-8 C overnight. For long term storage, adjust the ph value to between 7 and 8 and supplement with EDTA (final concentration 1 mm). d. To determine DNA concentration, remove an aliquot, dilute with water, and measure the A 260. For double stranded DNA, 1 A 260 unit/ml = 50 µg/ml. e. To calculate cell number, assume the amount of DNA for 10 6 diploid cells of human, rat, and mouse equals 7.1 µg, 6.5 µg, and 5.8 µg, respectively. f. Typical yields from tissues (µg DNA/mg tissue): liver, kidney, 3 4 µg; skeletal muscle, brain, and placenta, 2 3 µg. g. Typical yields from cultured human, rat, and mouse cells: 5 7 µg DNA/10 6 cells. To Amplify DNA by PCR After dissolving in 8 mm NaOH, adjust to ph 8.4 using HEPES (add 86 µl of 0.1 M HEPES, free acid/ml of DNA solution). Add sample (generally µg) to PCR mix and follow PCR protocol.
4 4 To Digest DNA with Restriction Enzymes Adjust the ph of the DNA solution to that needed for the restriction enzyme digestion using HEPES, or dialyze samples against 1 mm EDTA, ph 7 8. Allow the restriction enzyme digestion to continue for 3 24 hours under optimal conditions. It is recommended that 3 5 units of enzyme be used per 1 µg of DNA. Typically, 80 90% of the DNA is digested. Protein Isolation 1. Precipitate proteins (see note) from the phenolethanol supernatant (DNA Isolation, step 2) with 1.5 ml of 2-propanol per 1 ml of TRI Reagent used in Sample Preparation, step 1. Allow samples to stand for at least 10 minutes at room temperature. Centrifuge at 12,000 g for 10 minutes at 2-8 C. Note: For some samples, the protein pellet may be difficult to dissolve in 1% SDS (step 3). Use this alternate procedure to correct the problem: a. Dialyze the phenol-ethanol supernatant against 3 changes of 0.1% SDS at 2-8 C. b. Centrifuge the dialysate at 10,000 g for 10 minutes at 2-8 C. c. The clear supernatant contains protein that is suitable for use in Western blotting procedures. 2. Discard supernatant and wash pellet 3 times in 0.3 M guanidine hydrochloride/95% ethanol solution, using 2 ml per 1 ml of TRI Reagent used in Sample Preparation, step 1. During each wash, store samples in wash solution for 20 minutes at room temperature. Centrifuge at 7,500 g for 5 minutes at 2-8 C. After the 3 washes, add 2 ml of 100% ethanol and vortex the protein pellet. Allow to stand for 20 minutes at room temperature. Centrifuge at 7,500 g for 5 minutes at 2-8 C. Note: Protein samples suspended in 0.3 M guanidine hydrochloride/95% ethanol solution or 100% ethanol can be stored for 1 month at 2-8 C or 1 year at 20 C. 3. Dry protein pellet under a vacuum for 5 10 minutes. Dissolve pellet in 1% SDS aided by working the plunger of micropipette with tip in the solution. Remove any insoluble material by centrifugation at 10,000 g for 10 minutes at 2-8 C. Transfer supernatant to a new tube. The protein solution should be used immediately for Western blotting or stored at 20 C. Troubleshooting Guide 1. RNA Isolation: the final RNA pellet may not have been B. If the A 260 /A 280 ratio is <1.65: the amount of sample used for homogenization may have been too small. samples may not have been allowed to stand at room temperature for 5 minutes after homogenization. there may have been contamination of the aqueous phase with the phenol phase. the final RNA pellet may not have been C. If there is degradation of the RNA: processed or frozen after removing from the animal. the samples used for isolation or the isolated RNA preparations may have been stored at 20 C instead of 70 C as specified in the procedure. cells may have been dispersed by trypsin digestion. aqueous solutions or tubes used for procedure may not have been RNAse-free. formaldehyde used for the agarose gel electrophoresis may have had a ph value <3.5. D. If there is DNA contamination: the volume of reagent used for the sample homogenization may have been too small. samples used for the isolation may have contained organic solvents (ethanol, DMSO), strong buffers or alkaline solution. 2. DNA Isolation: the final DNA pellet may not have been B. If the A 260 /A 280 ratio is <1.70: phenol may not have been sufficiently removed from the DNA preparation. Try one more wash of the DNA pellet with the 0.1 M trisodium citrate, 10% ethanol solution.
5 5 C. If there is degradation of the DNA: processed or frozen after removing from the animal. the samples used for isolation may have been stored at 20 C instead of 70 C as specified in the procedure. samples may have been homogenized with a Polytron or other high speed homogenizer. D. If there is RNA contamination: there may have been too much aqueous phase remaining with the organic phase and interphase. the DNA pellet may not have been washed sufficiently with 0.1 M trisodium citrate, 10% ethanol solution. 3. Protein Isolation: the final protein pellet may not have been B. If there is degradation of the protein: processed or frozen after removing from the animal. C. If PAGE shows band deformation: protein pellet may not have been washed sufficiently. Appendix I. Isolation of Poly A + RNA After the RNA has been precipitated with 2-propanol (RNA Isolation, step 1), dissolve the pellet in poly A + binding buffer and pass through an oligo-dt cellulose (Catalog Number O3131) column to selectively remove mrna according to the procedure of Aviv and Leder. 3 II. Isolated RNA is to be used in RT-PCR 1. Modifying the procedure by performing the additional centrifugation step in the initial Sample Preparation, step 1B, note c further minimizes the possibility of DNA contamination in the RNA extracted by TRI Reagent LS. 2. A more complete evaporation of ethanol is required when RNA samples are to be used in RT-PCR. This is especially critical for small volume samples (5 20 µl), which may contain a relatively high level of ethanol if not adequately dried. References 1. Chomczynski, P., BioTechniques, 15, (1993). 2. Chomczynski, P., and Sacchi, N., Anal. Biochem., 162, (1987). 3. Aviv, H., and Leder, P., Proc. Natl. Acad. Sci. USA, 69, (1972). 4. Chomczynski. P., and Mackey, K., Anal. Biochem., 225, (1995). TRI Reagent is a registed trademark of Molecular Research Center. POLYTRON is a registered trademark of Kinematica AG. SPEEDVAC is a registered trademark of Thermo Savant, Inc. RC,JC,PHC 07/11-1 Sigma brand products are sold through Sigma-Aldrich, Inc. Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip.

domain: biology


text:
Ukraine’s burgeoning weapons industry
Re: Romania on Alert
The Fear of War Next Door
Ukraine: 1,000 Kilometres from the Front
Ukrainian deserters flee to Romania
Lviv - A Hospital in Wartime
In March 2022, the war between Ukraine and Russia is raging. While Russian troops are at the gates of Kyiv, this documentary films events in a paediatric hospital in Lviv treating the victims of the conflict. A powerful portrait of a doctor’s daily life in wartime.
DOCDAYS Productions GmbH

domain: history


CES begins tomorrow, that’s on 5th January, but there’s no announcement yet about a Samsung event that was being expected for the unveiling of the Samsung Galaxy S7. Well, it seems that the company is still not ready to launch its next flagship and we might have to wait for some more days. It is being said that the Galaxy S7 series will be unveiled at the Samsung Unpacked event in February.
Thankfully though, Samsung fans won’t get bored as leaks and rumors are pouring in from every direction. Today, a new report from Sammobile brings us a leaked screenshot, which shows that the Samsung Galaxy S7 will be sporting a 12.2MP primary camera on the rear. The phone will feature a 5.7-inch QHD display, the same size as the Galaxy Note5 and the Galaxy S6 Edge Plus. This 5.7″ variant might be the Galaxy S7 Edge.
The leaked screenshot tells us a lot of other details too. We get to know that the S7 will come with 4GB of RAM, 3712MB to be specific. A 5MP front camera will serve video chats and selfies. The phone gets the Adreno 530 GPU, which comes with the Snapdragon 820 CPU. A fingerprint sensor will also be there and will come with support for Samsung Pay. The Galaxy S7 might also get an iris scanner.


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domain: chemistry


- 100% Cairo long staple cotton terry, 625 gsm.
- White terry towels with mini stripe tape
- Bath Towels, Hand Towels, and Bath Sheets have three lines of tape
- Washcloths and Guest Towels have two lines of tape
- Tub mats are made with three rows of tape in a frame pattern
- Custom colors are available with a 25% up-charge and 3-4 week lead time.
- Made in the USA of fabric from Portugal.
Care: Machine wash warm. Tumble dry medium heat. Do not use bleach or fabric softener.
- Monogram Styles with an asterisk (*) can be ready in 2 weeks on Stocked Towels Only.
- Towels must be confirmed in stock by the vendor to ship in 2 weeks. If not in stock, lead time will be 3-4 weeks.
- All other monogram styles will be ready to ship in 3-4 weeks.
- Height is measured by center initial.
- Please note: Orders made exclusively for the customer cannot be modified or canceled after being submitted.
Colors: Blue Stripe, Green Stripe, Navy Stripe, Red Stripe, Tan Stripe
Sizes: Bath Towel, Hand Towel, Wash cloth, Guest Towel, Bath Sheet, Tub Mat, Quilted Tub Mat


With the casting vote of Rosio Torres, the congressional working group dismissed the case for the deaths of Inti Sotelo and Bryan Pintado.
- Council for the Reform of the Justice System ends without agreements due to lack of quorum
- Juan Silva: the criminal implications of the facts that allowed his escape
Manuel Merino was only a week as president before resigning. (Photo: Presidency archive)
The Subcommittee on Constitutional Accusations of Congress archived this Friday with eight votes in favor, seven against and one abstention the complaint of the National Prosecutor's Office against former President Manuel Merino de Lama and two of his former ministers for the deaths of the young Inti Sotelo and Bryan Pintado, during the November 2020 protests.
Initially, the vote resulted in a tie of seven to seven votes, so that the representative of the Alliance for Progress (APP) and president of the working group, Rosio Torres, used her casting vote, with which the balance tipped in favor of filing the case.
Con voto dirimente de Rosío Torres, subcomisión aprobó informe que recomienda archivar denuncia constitucional contra Manuel Merino. 8 votos a favor, 7 en contra y 1 abstención. Congresista Reymundo Mercado solicitó reconsideración de la votación. @Politica_ECpe pic.twitter.com/3wIImCPp1z
— Thalía Cadenas (@ThaliaCadenas) June 17, 2022
The complaint was presented in October of last year by the then prosecutor of the Nation Zoraida Ávalos, so that the investigations of Merino, the former head of the Ministerial Cabinet Ántero Flores-Aráoz and the former head of the Interior Gastón Rodríguez could continue. All of them have the right of preliminary hearing, so the file must go through Parliament.
This is how the subcommittee session went:
Merino, Flores-Aráoz and Rodríguez were charged with the alleged crimes of homicide (by improper omission) , serious injuries and minor injuries, due to the demonstrations in which 78 people were also injured.
The report that recommended filing the case was supported in the session by parliamentarian Alejandro Cavero, from Avanza País, delegate for this case, who during his presentation mentioned that the “unfortunate deaths” of the young people “were not the responsibility of the accused.” and that “police responsibility is not proven” either.
Also present at the session were the Democratic Change congresswomen Ruth Luque and Sigrid Bazán, who had previously requested to participate, despite the fact that they are not part of the aforementioned subcommittee.
The report was prepared by Congressman Alejandro Cavero. Photo: Congress/
Cavero's presentation was followed by a brief debate in which only the legislators María Taipe (Free Peru), Segundo Quiroz (Bloc Magisterial), Edgard Reymundo (Democratic Change) and Martha Moyano (Popular Force) participated. The floor was also given to Luque and Bazán, who – like their bench colleague Reymundo – criticized the report.
Bazán pointed out that in the document “the prosecutor's office is cited in some things, when it is convenient” and “in others it contradicts” the Public Ministry.
In this regard, Cavero replied that all the evidence and witnesses were “provided by the prosecution itself in the complaint.”
President Pedro Castillo went to the Attorney General's Office to testify as part of investigations against him for alleged acts of corruption committed in tenders within the Ministry of Transport and Communications (MTC). (Video: Public Ministry)
Congresswoman Moyano had a similar position: “The National Prosecutor, who is the one who accuses, has not summoned or requested, in any way, anyone on the part of the victims.”
After knowing the result of the vote, Reymundo announced that he would file a request for reconsideration with the aim of reversing the decision.
In a dialogue with El Comercio, Percy Castillo, deputy for Human Rights of the Ombudsman's Office, expressed the institution's rejection of this decision, which “fails to comply with the duty to exhaustively investigate any violation of human rights.”< /p>
Con esta decisión el Estado incumple deber de investigar exhaustivamente toda violación a derechos humanos. Evaluaremos las vías jurídicas pertinentes para corregir afectación a los derechos de familiares de los fallecidos y víctimas. #DerechoALaProtesta (2/2)
— Defensoría Perú (@Defensoria_Peru) June 17, 2022
Castillo added that “the Public Ministry and the Judiciary” are prevented from determining whether Merino de Lama and his ministers were responsible. The representative of the ombudsman also indicated that “Merino continues to be susceptible to other constitutional complaints being filed against him.”
Meanwhile, the Congressman Luque, from Cambio Democrático, considered that, with its actions, the subcommittee perpetuates “the shielding and impunity” with a “regrettable vote” of its president Rosio Torres, “who in her role should take part in the investigation that allows her to to the prosecutor's office to deepen “. “Mr. Cavero has decided to be a prosecutor, judge and expert and has rejected evidence,” he added.
This newspaper sought to contact Congressman Alejandro Cavero and with the parliamentarians who voted in favor of the report; however, as of press time, no response was received.
This was the filed constitutional complaint:< /p>


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This waterfront house is great for corporations and executives looking for a nice place to stay while working in the Hampton Roads area. It is also a great place for families as well looking for a furnished house to stay for a long term visit or for tempory work assignments. We are just minutes from the Norfolk Navy Base, ODU, Langley AFB, HU, Downtown Norfolk and many places in the Hampton Roads area. We are close to attractions such as Busch Gardens in Williamsburg, Jamestown, Colonial Williamsburg, Museums, etc. A great solution for short term stay in a nice house with all of the amenities needed instead of a hotel or small apartment.
4 bed, 3 bath, sleeps 10
- Calendar last updated:
- Dec 13, 2018
Check-in: 4:00 PM
Check-out: 11:00 AM
100% refund if canceled at least 60 days before arrival date. 50% refund if canceled at least 30 days before arrival date.
What A Way To Relax.
My family and I enjoyed our stay. The family room and dining area was very nice and spacious. Walking right out of the back door onto the pier was a plus. But best of all was the Oceanview Pier that was a little over a mile away - my husband and brothers really appreciated that. We had fresh spots and trout for dinner a couple of nights (yum). The ladies enjoyed the quiet and relaxation. Nice place to stay,!
We had a great time!
We enjoyed our stay in the place. It was a mini reunion of 2 families, some long some short stay and had a great time just staying inside. The house was clean although the furnitures probably need some upgrades. The teens enjoyed the backyard, biking to the beach & pier and kayaking and did fun catching crabs at the dock. It is a convenient location between VA Beach and Williamsburg and a walking 2 blocks away distance to a small beach.
I would stay again in this property if given the chance
spacious, comfortable, very enjoyable
we had a great time! for our son’s wedding, we had multiple families share this house and the one next door, Wil-O-Bay Getaway. we loved all the beach chairs and sand toys, 4 kayaks (between both houses) and a great big floatie, the pier and the crab pots, fire pits and wood. everything was clean, comfy, relaxing. it’s a quiet, private location with plenty of parking. the owner, Bonnie, was also easy to get in touch with and great for working things out. thanks for the wonderful memories!
The house was everything they said it would be! Wish we could of stayed lots longer
The bedrooms were all big and spacious. Really liked all the windows for the view of the bay. The kayaks were in use a lot when we had free time. Really enjoyed our stay.
Pleasant experience, as advertised.
We enjoyed our stay here for a mini-family reunion weekend. The space was sufficient for our family of 10. The backyard is a pleasant space for sitting and enjoying time together. The kids were able to do some crabbing and kayaking, and our youngest even caught an eel!
The location is a good jumping off point for trips around the area. The restaurants close by are friendly, neighborhood spots. The 7/11 around the corner is super helpful in a pinch.
The only drawback I encountered was the lack of cable or satellite TV for relatively important sporting events like the NBA playoffs and/or major league baseball. Still, when you're on vacation you're spending most of your time not watching tv, so this was a minor inconvenience.
This is an ideal location. The spectacular Willoughby Bay is in the backyard. Just steps away is the Chesapeake Bay Beach. Ocean view fishing pier is less than 1 mile away where there is an opportunity for deep sea fishing. The public boat ramp is less than a mile away with free boat launching and parking. Downtown Norfolk offers attractive sight seeing at Waterside and Towne Point Park, shopping at MacArthur Mall. The beautiful city of Virginia Beach is only 20 minutes away with a 2 mile boardwalk, bike rentals and excellent restaurants and shopping. Just through the tunnel about 30 minutes away is Busch Gardens theme park, Water Country water park, Williamsburg and Jamestown. You will not run out of things to do!
Additional information about rental rates
Rates are subject ot change and are only confirmed when terms are met. Please also check out our other vacation rental property located in Norfolk, VA - #678339 BB JJacks Lakehouse

domain: travel


Anyone who hasn’t just put their NAS in a storage room – if you have the opportunity there – will know it: So a NAS may not be exactly quiet. The hard disks make noises and, depending on the work rate, a fan doesn’t exactly run as quiet as a whisper. (Note: A member of our team has had a NAS in a closed office closet for years – with no problems from heat or anything.)
For those who are bothered by this, QNAP is releasing the TS-410E on the market. It relies on fanless cooling and can therefore also be operated in noise-sensitive environments – for example in the living room at home.
Inside the NAS there is an Intel Celeron J6412. The quad-core processor achieves a clock rate of up to 2.6 GHz. There is 8 GB of dual-channel RAM on hand. Four 2.5-inch SATA slots are available for SSDs. SSD caching can be flexibly configured to trade off IOPS performance against performance requirements. This is how you want to do justice to more demanding applications (transfer of large files, streaming and virtual machines).
And anything else? A hardware-accelerated AES-NI encryption is at work in the TS-378E. Two RJ45 ports with 2.5 GbE are available – the possibility of port trunking is given (up to 5 Gbit/s). QTS 5.0 runs on the NAS with various options for virtualization & Co. as well as applications (Plex server, etc.). There is also an HDMI output for direct 4K material.
TS-410E-8G: Intel Celeron J2022 Quad-Core 4-Thread Processor (burst up to 2.6 GHz), 8 GB RAM (not expandable), 4x 2.5″ SATA 6 Gbps SSD drive bays, 2x 2.5 GbE ports, 4x USB 3.2 Gen 2 (10-Gbps) ports, 1x 4K HDMI out
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Innowave Tech understands complete scope of automation. We minimize cost and disruption and maximize ROI through the system development and deployment with the right piecemeal approaches. Carries out comprehensive supervision and overall analysis of the production process, and forms a new mode of smart factory management.


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Pregnant in spite of plan b, nonchalance, magic and my non-pregnancy-inclusive plans. I had no idea. I understood in a quiet inner way that I only had a few minutes to get myself downstairs, and that I needed immediate help if I was going to live. A friend rushed me to the hospital where I, with no insurance, learned that I was pregnant and it was ectopic and I was lucky to live in a time when I could survive it. And I would be losing my left fallopian tube.
I rarely date men frankly it never seems to go that well, in spite of my earnest pansexual leanings. So rarely that when my dad heard the news, I think he seriously considered the possibility that I was involved in a biblical birth. When I got to the hospital, I told them I was pretty sure my appendix had burst. They said it was more likely that I was pregnant. I was adamant, I made my case of how that was impossible, asked them through clenched teeth to focus on the real problem.
I had two that night, humans who stepped over into a beam of light. I will forever be grateful for the convergence of events that led to my strange and lovely support team that night, and getting to see the particular goodness that can emerge in crisis. It was cold, and scary, the pain was nonstop, and there was a torturous internal ultrasound.
I both survived and increased my pain by laughing, and it was worth it. There was some time between learning I was pregnant and learning for sure that it was ectopic and surgery would be immediate, my hour of conscious pregnancy. I was in the middle of a book tour. So, if you want something to exist, you must first be able to imagine it. Therefore, the Great Spirit may show them many things that older people miss. For knowledge is limited to all we now know and understand, while imagination embraces the entire world, and all there ever will be to know and understand.
Occasionally, I find an answer. There are seven million. Are you faking it? A stick can be a magic wand. A sock can be a puppet. A small child can be a superhero. As they find out more about their world and their place in it; they work hard not to let adults hurry them.
Rumi quotes on life and self love
We need to hear their voices. You grow old because you stop laughing. I think this article is ridiculously stupid. Be exactly who you are. Essentially this article is asking you to be everything that human being are not. Just be who you are. Find people who like you, for you! Someone asked about applying this to parenting. My daughter sent it to me, and I am sending it to my other daughter and sister. I have been working on most of these, and know they are all helpful when we are open to the comments.
Find the good and worthwhile and leave the rest for someone else. Nuf said. Thanks so much. Thank you. My husband has been out of the house for over a year now and has made my life hell. I often wondered if he had a mental illness, not because of my anger but because of some of the things I witnessed during the last two years of our living together and his complete erratic behavior now. I think I also suspect he does because some of the same behaviors I am noticing in my son who has been tested and shows signs of ADHD.
Great article! For me, the difficulty was learning HOW to give these things up. Just announcing I was giving them up, or trying to force it, fake it, or beat myself up for failing never worked. This is amazing knowledge posted here. Thank you again — Keith. The article should open with the premise that people suffer when they are self-absorbed, self-centered and self-focused.
What an overly simplistic representation of the world! Most of your points seem to imply that ignorance is bliss — not something we want to teach future generations. There is a way to be happy, and to find beauty in the world AND criticize it so we can make it better. Complacency is not the answer to happiness, betterment is. In order to create something better, you first need to let go of the not so good in your life.
If you want to new furniture in your apartment, you will not put the new one on top of the old one, right? I must say that everything is tied together such as stopping making excuses will help with ego. We protect our ego with our excuses. One more that we should give up, is: Give up being selfish. Being selfish is the first step to unhappiness. We believe we will be happy if we give to ourselves. The reality is that we reflect our emotions. So when somebody does something good and makes us smile, we get a deeper sense of happiness. If we all do it, we can all be happy.
Ultimately, being selfless is the ultimate act of selfishness, since through our actions of kindness we receive more love and in turn we become happier…. I have trouble giving up attachment to the relationship with my son. I agree. They each have their own rules, some of which overlap. CRITICISM is one of the most self destructing tools you may use, however high u hold your ideologies, be sure that they are not as high as you think of them!
Hi Purpose Fairy, and I must admit it seems strange writing that; I had a friend on Facebook add a link to this article and I found it useful and accurate. After reading a few of the comments, I thought it may be valuable to add that I have a website and blog, Eight Facets, which concerns what I think is the most effective and quickest way to change outdated habits into ones which lead to happiness.
I have written a book on the topic of just this, which was published in February. Thanks again. And bravo for writing it so simply and matter-of-factly, just like it should be practiced. I agree with all of these except That is the stupidest concept I have ever heard of. Attachment IS part of love. If you deattach from everyone and everything in your life — where will you be?
Obviously its very unhealthy, so why would you encourage people to do something like that? Laura, when you are attached to things, people places, you do it from a place of fear, fear that you might one day lose all of them, and that is now real love. It does not envy, it does not boast, it is not proud. It is not rude, it is not self-seeking, it is not easily angered, it keeps no record of wrongs.
Love does not delight in evil, but rejoices with the truth. It always protects, always trusts, always hopes, always perseveres. Love never fails. Being detached from them does not imply you love them not, on the contrary, it shows a great deal of wisdom and understanding about life. You can read more about this here:. I agree with you, Laura. Decades of psychological research has demonstrated how essential it is for people to form secure attachments to others.
We are all born hard-wired to attach, to form into groups, tribes, families… Connections and attachments build communities, promote compassion and empathy, and give a sense of identity and belonging that all people need. Yes, attachments may open you up to pain in this life… but that is not a reason to avoid them in the pursuit of happiness. Most of the things on the list are fairly easy to give up. Thanks for the reminder! Fabulous post. Not patting myself on the back here, just sharing my experience.
I clicked on this thinking it would be about giving up smoking and dairy and stuff but this was much better:. I, recently, had a breakthrough! I decided to give up on what people were telling me to do and do what I really wanted. I believe that God has more me and am going on a year-long mission trip. I would love if you followed my journey. Why does everything have to revolve around happiness? As if happiness itself was the ultimate goal of existence. Happiness is the name we give to our half witted understanding of that part of the complex cognitive machinery that in one way or another makes us get things done.
Happiness is not an insatiable god we have to worship. Give up being right? Why would I? Give up control? This sort of hippie buddhist laissez-faire sounds totally counterproductive to me. Things would never get done with that strategy. This is a first step to change things for the better. Pretty much agree with everything you just said, particularly re.
My favourite point by far is Interesting post and comments. However, the title itself made me recoil. It is very destructive indeed. There is wisdom in forgiveness and turn the other cheek. Living the examined life is paramount, and part of that examination is the acknowledgement of evil and sadism in the world.
Right now, organized groups of men are seeking to harm women through ugly legislation. Now, once we have taken the time to listen, and understand that these men are truly dedicated to harming women, we are left with very real and stark choices. We can look away. We can not be critical, and hope that things improve. Or we can look, reason, use every sense we possess, all our experience, our empathy and self-preservation, and stand against the evil.
I cannot stand by and watch the progress we have made harm my daughter. Trust, but verify, THEN look on the bright side.
- The Perennial Wisdom of A Course in Miracles?
- Game of Souls (The Quintessence Cycle Book 1).
- Water-Insoluble Drug Formulation, Second Edition.
Oh dear! My apologies. My comments on fear was in regards to 11, not 10, as I noted. So sorry. I would like to say that 10 is not a wholly healthy perspective. There are things you should generally be concerned about that are real threats e. Thanks for your input. Therefore all progress depends on the unreasonable man.
I gave up on all those things and guess what?! Oh if i could but do half of these things. My great big FAT ego allways stands out in front and acts like the coolaid guy bustin in the wall!!! Well done. I would add this… Give up trying to do everything at once. My experience of people who follow this way of life is that they are very self centred and uncaring about other people, not my idea of an ideal parent or partner. The only way of living with them is to become like them and that does not appeal to me.
I also believe the world would be a worse place. On the surface, I thought this was mostly pretty good advice. I know someone who could benefit. But this person has also been very self-centered, and following these tips would do nothing but paper over that problem. Wonderful to read this this morning. Trying to find yourself? We do not have to search for ourselves, because when we become aware that is our true self, the start anyway. I cannot explain it very well, but on reading his books, have found true peace with myself, my true self. I think no. Critical thinking allows one to elevate beyond the criticism of differences, and to start asking why things and people are different, what that means for who I am, and what that means for my place in the diversity of the world.
The trick is to strike a balance between that type of critical thinking and having a clear happy way of being. The type of criticism to give up is the the kind that is unproductive. Looks like you have some great insights and essays. Are you a life coach? Have a great one! Happiness is were I will be and if it causes stress Im learning to walk away.
To be happy, one just need to be content on what they have. It easier said that done. Technically this is more achievable. Love this post. Now i have a list tackle it all! I have some relatives with mental illness. Their illness has become part of my problems too. Please believe me that I know it is tough and they have been dealt some bad cards. I try to understand and help when I can. But tackling some, not all of things on this list, is helpful for everybody.
I so like this! I have been working on all of these. Can you plz elaborate point number 14 with a very thought provoking example. Will be really great. I love this post but you can do or not that the point we are human being but I will try ,when I read this to my love he give me a lovely look. You are so right. Oh, I know that I have overcome even these things, as a great many have everyday of their lives or every other day, should I say.
There is also, I believe the agreement each of us made before coming into this world, to be in a situation or with certain people that we can help to learn. I have a problem with what is referred to as the subject of attachment. People are devoid of attachment, when we are speaking of persons, places or things. What this means is some people not all but there are some which need validation, but do not require it in another form or other.
I am aware that infers a contradiction of the sort. What we can draw to a conclusion is that there exists a conflict, or a struggle to identify what acts as attachment then from not. If you read me properly, I feel attachment on many different levels but it DOES nothing to inform me from the benefits of it. I may see attachment in the manner which I seek it. Therefore, I see some superficial aspects as well.
I call that a double standard. To approach the various levels of attachment I care to submit myself to, I know I speak in a language unfamiliar to incur what those aspects actually may be. Maybe they are true, maybe they are not. I feel insecurity just like all people do, in order to identify if they person x y or z only rather benefit from it themselves without expending anything at all.
Attachment resembles a quality of something along the lines of inferiority, that people aim with, for something they want not that THEY need. What a great article. Take control of your mind. Your mind is to serve you. To give you answers to your questions and retrieve things in memory. Stop it from controlling you with its thoughts. Put your attention on your immediate present and dismiss interfering thoughts your mind produces to take you out of presence. Recognize the fears for what they are and dismiss them rather than entertain them and let your mind control you with fearful thoughts.
Focus on the pleasures available to you in each moment. Focus on the positive and make note of the negative. Focus on solutions to problems and take your attention off the problem itself. Recognize, acknowledge and dismiss the fears underlying emotions that arise when you think about the problem. Start smiling.
Force yourself if you have to, but keep it coming because it will eventually flow freely. Every time a negative thought or statement is delivered by you or someone else, create a thought or statement that is the exact opposite. The opposite must exclude the original negative word. Every word you speak is recorded in your cells. Negative, words, statements and thoughts lowers your vibration. When you think positive thoughts, take positive actions and speak positive words you raise your vibration. Separate yourself from negative people and situations.
Think of yourself first. Take a stand for yourself. Treat yourself with the utmost respect. Recognize when others are trying to engage you in their negative creations and patterns, and disengage quickly. Walk talk and be proud. Reward your loving self and stop punishing yourself for negative thoughts your mind creates.
Recognize the behaviors you have programmed into you as a result of fears created by your mind.
Let go of those behaviors and let your loving heart — the true you before all the bullshit, be the one that participates in and enjoys life and is the one everyone gets to know. I like how anyone who disagrees with you gets deleted. Do not believe in anything simply because it is spoken and rumored by many.
Do not believe in anything simply because it is found written in your religious books. Do not believe in anything merely on the authority of your teachers and elders. Do not believe in traditions because they have been handed down for many generations. But after observation and analysis, when you find that anything agrees with reason and is conducive to the good and benefit of one and all, then accept it and live up to it. From personal experience — I believe suicide is a call from the spirit world when are ego is not happy with the matierial world.
My mental illness grew from delusions of grandeur I my ego spent years cultivating. I do believe the spirits help us on this plane when we are open to the next demension. The Course of Miracles is a great way to see with Love rather than the illlusions of fear. Love is in the air — breathe!
5 Tools For More Miracles Now - Elyse Santilli
I can be a tad bit cynical. This is like all those people running around trying to get into heaven. What control queen wrote this nonsense? Thanks for this! I now have a huge piece I can make for my office wall! I think I need to copy this and carry it around with me so I can read it daily. Thanks for sharing. This is just what I needed today! Great article, thank you so much. I recently got out of a relationship and a LOT of the above listed were present in the relationship. It hurts right now very much but I know it was for the best. I see in the post s that there are others out there that are working on finding a happier place, much as i myself am.
I would like to suggest that those of us with problems with any of the above should sit and contemplate how these affect us and how each of us as individuals can make them work for ourselves rather than pick them apart and try and make a bad situation out of a well meant and helpful posting.
The author is not perfect and would probably not claim to be, merely trying to help there fellow travelers down this often confusing road that we call life. Thank you Lucius for putting into words exactly what I would tell others. NOT excuses! After self medicating for 15yrs. I knew I needed help. Been on meds never abused them , and in Therapy off and on for almost 30 yrs.
I could not count the times I wished, prayed, I was physically ill with something instead of my mental illnesses. I am still a Christian, and know someday I will get answers for this. I know God heals. I have seen miricles. Just not my time. I also know God did not cause the abuse to happen to me, it was a choice of my parents to do what was done.
I know others have been through worse. I left church 3yrs. I know I have to forgive, to be forgiven. God help me as You have all my life. You are not alone. But do seek help. I have seen it work for others. Never give up! Each afirmation makes it that bit closer. Thank you for post such a wonderful blog. Keep up the great writing! And thanks to this article I can add to my list. Thank You for this article, Purpose Fairy. I remember a few years back when I was just as how you described.
Somewhere during that time probably because of my stressful job , I lost my way and became a lot more bitter and upset than usual. A lot of these action steps will help me better myself and bring out the old side of me again and help move forward with my life. Great list, and awesome insight. Some I have been able to let go, but others are still a challenge. What a great list. I have been working on many of these, and your list brings a few others to mind. I will print this out and put it somewhere I will see it frequently.
The loss of a pet is a reason to grieve and to mourn. Those are okay. And, believe it or not, it is also okay to laugh when you hear that someone you loved passed on. Use one suggestion to live into another:. Give up your attachments that say Death is a Negative.
How to Manifest Life-Changing Miracles
Give up living up to expectation, it says. Give up on the fact that trauma defines who you are. It does not suggest these are easy tasks! They apply to the Big Stuff, too. Be challenged by what you read here; if you need a blog to tell you to stop complaining, and blaming other people, so be it, but that is not what it was written for. But the sooner one is willing to face the past giving it up would be to reject the experiences that formed you, which would leave you without a reference point — why would we want that?
Give up on your fears. Fear is not an illusion. If we create it, then it exists. Thank you Lucius for your clarity, it has been insightful-dealing with lost friendships… Purpose fairy is a wonderfully appropriate name, god bless. Lucius, I absolutely agree to you response. Also, Chrisfer, I, too was hurt when I read comment When someone has a mental illness, they still strive to better themselves.
Many mental illnesses can be treated with medication. Having said that, I must add that when a mental illness is made stable through medication, that person who suffers from it oftentimes wants to feel normal. An article such as this would then target them as an audience just as much as a person who is not mentally ill. Danaadmin, please do not exclude people that suffer from a mental illness. You cannot possibly understand them unless you have been in their situation. Otherwise, keep writing uplifting articles.
Just remember to carefully word your responses to the resonses. There is a few I have to work on. Because of our talk, I thankfully have given up quite a bit. I relied on others for information. NOW I have the only one in me heart to go to and who is the chief healer. I, as we all should be going to the Lord forever. In the world we look at so many different views and have not only our habits, but seem to take on others.
Again, thank you. Previous therapy sessions never touched me as deeply as this piece just did. Although everyone could benefit, this should be required reading for ACoA. Since I doubt anyone is going to disagree with you, while I agree with most of these some of these are not only counter-intuitive but are also anti-motivational and simply put, horrible advice. Also engaging in short term things that make you happy will have long term consequences. Give up the need for Control: this should be give up the need to control external things.
Yes you might be happy shoving your face with ice cream, but ultimately this lack of personal control will lead to obesity and sadness. Give up complaining: in certain contexts you definitely should complain especially when it has impact. If the government is doing something that is going to destroy your life, again complain.
There is a certain amount of complaining that is necessary otherwise you will become apathetic. There is nothing wrong with going the extra mile because you know it will impress people. Putting in more time in the office, or spending more time at gym. While these things might remove short-term happiness they can give you greater long term happiness. This should be available in a large poster format. It would be a perfect constant reminder plastered on my office wall. This post does an excellent job of speaking to all people who, at one time or another, have experienced and can relate to all fifteen items it addresses.
I think for people who have been diagnosed with having a mental illness, continuing to communicate with a professional is a helpful solution and should be continued.
- The Heretical Archive: Digital Memory at the End of Film;
- Mein Körper in neun Teilen (German Edition).
- Miracles Quotes ( quotes);
- What is the Law of Attraction?;
- Three Men in a Boat;
- Explore Topics.
However, if you think that this post does not relate to you then you are mistaken. To think that your illness will not allow you to act on these lessons as they are presented is a falsehood. I have been depressed and suicidal myself. I have experienced the transformation first hand of rising out of the darkness into light. For others in dark places or have had a doctor tell them that they have a chemical imbalance that causes them to act a certain way, it is never too late to change your brain. The human brain is elastic and it can be changed. It can be changed into something entirely different through training and practice.
Never give up on hope and never limit yourself or deny your ability to becoming a positive, happy person. With the exception of severe trauma or a physical impairment within the brain, the only limitations in life are self-inflicted.
- Tag: miracles.
- Petit Pierre, il était une foi (French Edition);
- Sagittarius: Personal Horoscopes 2012;
Our minds can just as easily think positively as it can think negatively. Considering this, the satisfaction of life depends solely on the choices that an individual chooses to make and how they react to those choices. Be well and choose to see life as it is and nothing more. Live simply, slowly and always be aware and observing of the environment.
I read this whole article today, and I am so glad that I did. I have lost who I am, and what it is I enjoy, besides being a Mom, I really have no other joy in my life. My life has almost always been about doing for others……..
Membership = LOVE
I have always thought that it was a selfish act to think only about what I want and enjoy. My life has taken many, many directions in my 51 years and it is hard to stay on the path that takes me where I need to be. Old habits are very hard to break. I literally make myself sick over what to do to change this mindset. I am ever so gratefull for the person that sent this to me right now am dealing with a lot of stuff that was mentioned here ,this has open up a new way of thinking for me ,am happy that i took the time to read it through ,i plan on reading this everyday until i come to the place that am suppose to be at ,thank you honey thank you…..
I will take a mental note of what happened and why, learn from it and move on. I cant just put myself first and be true to myself. I cant, there is too much pain in this world. To Lucius, I was happy to see your thoughtful and eloquent response. I have been dealing with depression all my life and have been diagnosed since the age of It took me many years to understand why I was often moved by something but unable to apply the principles that impressed me in my life.
I could have saved myself years of frustration. What I needed to be reading was works by wise people like you: my kind of normal. Is this what poor people do to pass the time? Happiness is life without worry, without concern over serious problems, and the only thing that fixes that is money. Literally all these issues are alleviated if you have enough money. I would add just one more to this list. And that is to remove the word hate from your mind. If there is no hate then only true love can exsist. Try not. Do… or do not. There is no try. These are interesting and philosophically curious.
There is a fine line between spinelessness or indifference and blissfulness. This line is not absense but presense, which often takes a great deal of control—self-control not control of others. But, what do I know. I am facing my second divorce and have spent much of my life soul searching. This is a fabulous post!

domain: literature


text:
Depp is suing ex-wife Heard alleging defamation and damage to his Hollywood career as a result of her op-ed in 2018 in The Washington Post, in which she alluded to being abused by the actor without naming him directly. The trial started on April 11 with jury selection and is expected to last a total of six weeks.
Heard countersued her ex-husband for double the amount, accusing him of allegedly orchestrating a “smear campaign” against her and describing his own lawsuit as a continuation of “abuse and harassment”.
The Oscar-nominated actor will reportedly testify today, Tuesday 19th April, and will return to the stand on Wednesday to be cross-examined by Heard’s attorneys.
And Pogues frontman Shane MacGowan, has shown his support for his “brother” Johnny as he prepares to take to the stand today.
Taking to Twitter, Shane shared a photo of the pair and wrote: “Stay strong brother we are with you.”
Last week, the singer’s wife Victoria Mary Clarke also posted a message on her Instagram page in support of Depp.
Clark shared a picture of Depp alongside Gina Deuters and the message: “Sending so much love to Gina @girldeuters today!!!” with the hashtag #johnnydeppisinnocent.
Depp and MacGowan have a close friendship that goes back more than 30 years, with Depp producing the 2020 documentary about the musician’s life and career: Crock of Gold – A Few Rounds with Shane MacGowan.
Speaking at a press conference ahead of the film’s release back in September, Depp recalled the moment he met MacGowan for the first time and said that he “fell in love with him.”
The Pirates of the Caribbean actor said: “When I met Shane, he was negotiating a pool table. There was a drink in this hand, a pint, and in this hand, there was a guitar.
“And he was teetering, balancing back and forth trying to negotiate which way to fall. I watched him do that for about 15 minutes.
“Then I was introduced to him, before he fell, and from that moment on you just knew... there are moments in life when you know this will happen one time and one time only, when you get the opportunity to spend time with greatness.
“Having known him a long, long time I can only say I fell in love with him the second I met him and I’m still in love with him to this day.
“His wife Victoria is a wonderful partner for Shane, they’re best friends, and I’ve seen that continue over these many years under all kinds of duress,” he added.

domain: artistic


The Greater New Orleans Housing Alliance is urging Gov. John Bel Edwards to veto legislation that would ban local governments from passing zoning laws that would require developers to incorporate affordable housing into new projects. The Louisiana Senate gave final approval the bill Wednesday (May 16).
The New Orleans City Council opposes the bill, and the Greater New Orleans Home Builders Association supports it.
Senate Bill 462 removes language in a law that references "inclusionary zoning" and changes it to "voluntary economic incentives." Cities, including New Orleans, have considered whether to provide tax incentives to get developers interested in building affordable housing, but inclusionary housing policies are more restrictive. They typically call for a certain percentage of units set at low-income rents or selling prices in new residential developments.
In New Orleans, a "Smart Housing Mix" was proposed by the City Planning Commission that would make developers eligible for density bonuses -- adding more units overall -- if they agree to include a percentage of affordable housing in their projects. The City Council never acted on the Planning Commission's proposal.
Andreanecia Morris, executive director of the Greater New Orleans Housing Alliance, warned in a statement about the damage the bill could do to efforts to make housing affordable in the state's largest cities.
"The wide ripple of effects that will undoubtedly occur from the implementation of this bill will be devastating for our state as New Orleans, and many other cities throughout Louisiana are in the midst of an affordable housing crisis," Morris said. "The Greater New Orleans Housing Alliance, alongside our many local and statewide affordable housing advocates and allies, are deeply disappointed in the decision of our state government to allow this bill to pass."