The TLS protocol secures the communication between a user’s web browser and a server running a web application. The user recognises a secured communication by the lock visualised in the web browser or the
https prefix in a link.
The security protocols TLSv1.0 and TLSv1.1 are outdated and no longer rated as secure. Therefore, web server administrators should plan to properly protect their services by updating their web server configuration to require at least TLSv1.2.
To apply this security improvement to SWITCHaai including SWITCH edu-ID, SWITCH announces the upgrade in two phases.
First Phase: IdP Login Pages
In the early morning of Wednesday 2. July 2020, SWITCH will update the web server configuration of the SWITCHaai Identity Provider (IdP) systems operated by SWITCH to require TLSv1.2.
Users who login with a web browser that uses one of the outdated security protocols TLSv1.0 and TLSv1.1 will be rejected. These users then need to switch to another web browser or update their operating system to get a newer version of their default web browser.
This change will be applied to the following Identity Providers:
- SWITCH edu-ID including the Identity Providers of all these organisations having adopted SWITCH edu-ID:
- FernUni Schweiz
- SLSP – Swiss Library Service Platform
- SWITCH Staff
- Université de Fribourg
- Universität Luzern
- Universität St. Gallen
- ZHAW – Zürcher Hochschule für Angewandte Wissenschaften
- ZHdK – Zürcher Hochschule der Künste
- Virtual Home Organisation (VHO)
- The SWITCHaai Identity Provider Hosting customers
Users are already warned
Since June 2020, users logging in with an outdated web browser not supporting at least TLSv1.2 have seen on the above listed Identity Providers such a prominent warning on their login page:
Very few users will have to act on it
The analysis of Identity Provider log files revealed that only very few users still connect via TLSv1.0 or TLSv1.1. They mostly use the default browser on no longer supported operating systems like Android 4.4 or Mac OS X 10.6 – 10.8, likely private devices. As immediate fix they could download and install e.g. a Firefox browser. For security reasons however, they should better upgrade or replace their no longer supported operating system with a current version.
Second Phase: The SWITCHwayf Discovery Service
In the second phase, the outdated versions TLSv1.0 and TLSv1.1 will be turned off also on the SWITCHwayf Discovery Service on Wednesday 5. August 2020.
Since mid June 2020 users who should act see the TLS warning also on the central SWITCHwayf Discovery Service:
Others already did it
SWITCH is not the first Swiss organisation enforcing better security for user login. The following Swiss e-banking login pages already deny access via TLS 1.0 and TLS 1.1: Credit Suisse, PostFinance, UBS and ZKB.
Google announced to remove TLSv1.0 and TLSv1.1 from Chrome 84 in July 2020. Also Microsoft will disable TLSv1.0 and TLSv1.1 for Edge. Firefox and Safari had it planned for March, now postponed due to COVID-19.
Update: Firefox 78, released on 30. June 2020, disabled TLSv1 and TLSv1.1.
TLSv1.2 has been enforced since a few weeks on the SWITCHaai Resource Registry web application, the AAI Attribute Viewer as well as on the WAYF-Test server. We haven’t heard yet of any related end user issues, neither about the TLS warning as presented on the Identity Provider login screens.
Therefore, we are even more confident that only a very limited number of end users will have a problem at all.
Plan to upgrade your own web servers!
Let me give you some recommendations based on the learnings while planning this upgrade.
Check the current status with the SSL Server Test provided by Qualys SSL Labs. If the rating reported is ‘B’ or lower, then it’s time to plan an update. The best rating possible is ‘A+’.
For many server platforms, the Mozilla SSL Configurator provides valuable hints. Our setup is primarily based on Mozilla’s ‘intermediate’ configuration. However, we activated the server-preferred cipher order and
ECDHE-RSA-AES256-SHA384 as additional cipher suite, in analogy to major e-banking sites.
Web servers with a very broad user base are more likely to serve users with outdated browsers. Therefore, check out your web server logs to find out how many users connect with outdated TLS versions. Perhaps you need first to activate the logging to also include the TLS version negotiated.
If you barely see any outdated TLS connections, you could consider a direct upgrade. Otherwise, try to inform in advance those users who will have to act. If a user’s web browser is outdated and TLSv1.2 is enforced, the user will not be able to establish a connection to your server, which prevents you from displaying a tailored error message.


text:
Institute® and The Freeman Institute®
have combined resources to launch the free man
publishing (fp) enterprise.
A curiosity-driven multi-media publishing company.
you tube videos
black history resources
E X E C U T I V E
S U M M A R Y
freeman publishing is fueled by some big ideas ]
curiosity-driven resource is found in two editions of the
same 192 page book, "God Is Not Fair:
Coming To Terms With Life's Raw Deals."
Coming To Terms With Life's Raw Deals) has been designed
for the general marketplace. The other edition (subtitled: Making Sense of Loss and Grief)
has been private-labeled by National HME for the
hospice community. This particular edition was
editions are designed for people who are in any of the
"before-during-after" stages of pain: loss of a
loved one, cancer or some other disease, wrestling
with the "why's" of tragedy, parents dealing with
rebellious children, paralyzed, struggling with
conversations/events/decisions that have negatively
impacted relationships, prison sentence, periodic
depressive state, fear of death, discouragement,
strained relationships within a family system, dealing
with life-controlling thoughts or temptations,
approval addiction issues, bitterness, shame, dealing
with narcissists or those with a heightened sense of
personal entitlement, and so much more...
book will also be of special interest to: hospital
chaplains, therapists, prison chaplains, clergy,
military chaplains, psychologists, pastoral counselors,
parents of teenagers, guidance counselors,
spiritual advisors, mentors, social workers, and anyone else in the people-helping
profession -- looking for resources to help people
walk through grief and loss.
has already been a bestselling book for 25 years, with over
400,000 copies worldwide in some 20 foreign
translation editions -- endorsed by Billy Graham, Joni
Eareckson Tada, Paul Meier, and many others. The two expanded and updated 25th anniversary editions of
"God Is Not Fair" were released
The audio book,
mp3 and ebook editions will soon follow. We already
offer an edition for youth and also a hard-cover
gift edition. Read the back cover copy below.
It is important to communicate that free man publishing
(fp) is committed to the highest quality of
product and customer service. fp is willing to work with any organization
desiring to develop their own "private-labeled" edition
of this or any future resources developed by fp. We
establish exclusive relationships with only one entity
per profession. For instance there will only be one
organization selected for the hospice industry, prison
outreach, or some other category, association or
organization. fp is also willing to having any edition
translated and published anywhere around the world.
|special hospice edition
So, What Did You
Sometimes life isn't fair, but we expect it to be. Why
else would we be so angry and hurt when it isn't? We
- If God is a God
of love, why is there so much suffering in the
- Can any meaning
be found in pain, loss and suffering?
- Why do evil
people seem to prosper?
- Why does life
have to hurt so much?
- Why do terrible things seem to happen to
- Why does life have to hurt so much?
- Isn't there an easier way to grow?
- Can any meaning be found in pain, loss and
Written in a highly readable and
thought-provoking style, Joel Freeman encourages
readers to seek a greater understanding of their
feelings of loss and grief through personal
reflection. He also prompts discussion about their
expectations of themselves, God and others.
This book is not filled with glib answers, which serve to drive
honest strugglers into deeper disillusionment. Drawing
on his years of experience in counseling people
through their grief and suffering, Dr. Freeman
provides a framework upon which can be found true,
lasting peace and security.
Endorsed by Joni Eareckson Tada, Billy Graham, Paul Meier and
others, "God Is Not Fair" presents helpful insights,
poignant stories and touches of humor in revealing a
biblical perspective on these questions. You are about
to embark upon a remarkable journey.
books (tentative titles) will be released soon by free man
Dealing With the People
Who Drain You of Your Time, Energy and Patience
The Mysterious Rosetta Stone
Dealing With the Wolves, Serpents
and Swine in Your Life
Man's Journey Into Black History
An Eye-Opening Experience That Transcends Race
Blades of Grass in a Concrete
The Surprising Benefits of
The future multi
media publications will prove to be even more
intriguing. The resources
published by free man publishing are
designed to address “felt-need” issues, reader/user
friendly, multiculturally astute, and timely, yet
Here are the
types of resources to be published in the future:
you tube videos
black history resources

domain: literature


Award-winning: all-new Nissan NP300 Navara wins International Pick-up Award
- NP300 Navara scoops top honour at the International Pick-up Award 2016
- Production commenced at Nissan’s Barcelona plant this month and arrives in UK showrooms in January 2016
- Judges praised striking exterior and interior, new-to-sector technologies, 2.3-litre dCi engine and impressive payload and towing capabilities
The all-new Nissan NP300 Navara has won the coveted International Pick-up award 2016 at a ceremony in Lyon, France.
Nissan’s recently-launched pick-up fought off stiff competition with judges praising its superior on-road and off-road capabilities, strong engine performance and payload capacity.
The new NP300 Navara underwent the rigorous International Pick-up testing process, each being judged for its off-road ability, on-road performance and its value for money. They were also tested on their ability as a workhorse, which included comparing towing capabilities.
The new NP300 Navara delivers new levels of crossover-inspired comfort and refinement while maintaining the Navara’s acclaimed off-road ability, durability and load capacity.
Its robust chassis introduces a host of new features, including an all-new five-link rear suspension for the Double Cab model and an impressive load bed, which is 67mm longer than the previous model.
The wealth of innovative and affordable technology such Around View Monitor (AVM) and Forward Emergency Braking (FEB), designed to further improve driveability and practicality, are also unique to the pick-up market.
Philippe Guérin-Boutaud, Corporate Vice President Nissan LCV BU, said: “This prestigious award celebrates Nissan’s 80 years of pick-up expertise. Rigorous testing by International Pick-up of the Year jurors illuminated the tough and innovative character of the all-new Navara.”
“Nissan is best known by its customers in Europe as the maker of capable 4x4s and breakthrough crossovers. This new Navara represents the fusion of those two realms of excellence to deliver a truly breakthrough pickup truck that combines rugged 4x4 toughness with the sophistication and efficiency of a Crossover”, added Ponz Pandikuthira, Director of Light Commercial Vehicles for Nissan Europe.


What is it?
We’re quite familiar with the Panamera. We know it has its strengths in its performance and handling, and weaknesses in its comfort and interior practicality. But if there is any model likely to hit a sweet spot amidst all the compromises, it will be this naturally-aspirated V8 4S model, complete with 395bhp being sent to all four corners via Porsche’s double-clutch PDK ‘box.
Our car also came with the £750 Sports Chrono plus pack, which (in conjunction with the various standard electronic safety systems) brings with it launch control and more focussed settings for the gearbox, steering, traction control and throttle response.
What’s it like?
Very good - the best in the range, in fact. It’s the powertrain that really makes the difference here. Yes, it’s less brutal than the Turbo but for a car that has its roots in the luxury car market the wonderfully linear, accessible delivery from this 4.8-litre V8 is spot on, and regardless of where your performance benchmark sits, 4.8-seconds to 62mph in a 1.9-tonne car is hardly tardy.
Stop-start is standard on this model, and was one of our biggest gripes in the Turbo because it lacked much cohesion in conjunction with the PDK ‘box at typical town speeds. In the V8 this problem seems to have been ironed out to the point where it is a rare occurrence to be left hanging at a junction for an awkward moment whilst you wait for the engine to kick in following a prod of the throttle. Generally, even in creeping traffic, the stop-start system is something you can forget about and it just does its business. And after all, you can turn it off should you want to.


Tag: Tesla recall
If you own a Tesla Model S or X, then your vehicle might be part of this voluntary recall related to an issue with the parking brake.
Tesla has issued a recall for certain charging adapters due to potential for overheating / possibility of fire.
On Friday, Maya Kazan asked Elon Musk on twitter if rumors were true whether there is a five week wait to get into the shop in some Tesla service locations...
The Tesla Model S seatbelt recall affects all 7,166 Model S sold in China.
Tesla has recalled every Model S ever made over the potential for a safety issue linked to seatbelts.
Toyota is recalling its electric RAV4 EV to fix a faulty Tesla-supplied component.
After a recent accident that saw a Tesla Model S ignite after running over a tow hitch (which impaled the bottom of the car and damaged the vehicle's 85...


Before beginning the lesson, I ask the student what he or she wants. What does he or she specifically want to learn or gain from dance lessons? My lessons are completely tailored around my student's wants and needs. Dance is supposed to be a fun, relaxing form of expression, so I like to help my students express themselves through movement in a way that is comfortable and fun for them.
I have been dancing in some form or fashion since the age of 3. I did ballet and tap recitals in preschool, took recreational gymnastics and cheerleading classes in elementary school, and did allstar dance and allstar cheerleading in middle school and high school. When I went away to college, I continued to take classes in many dance styles when I had time. I have never lost my love for dancing. My favorite styles are modern and ballet. I currently own a studio in the DFW metroplex and am excited to expand my teaching to online students!
I am a teacher of twelve years and I love what I do!
My hop hop lessons focus on conditioning, isolations, fluidity, musicality, and strength. This can include warm up, stretch, technical exercises, across the floor, choreography, and freestyle. My main goal is that my students have fun in addition to benefitting from our lesson. All my classes are personalized to suit my students' goals, progression, and timeline.
Hip Hope Dance lessons are offered at 4 locations: 1) Online via TakeLessons Classroom 2) Teacher Location 1 (Primary studio - 150sq ft) - For private and semi-private (2 people) lessons 3) Teacher Location 2 (Secondary studio/theater 350-750 sq ft) - For Group classes (3 or more people)* 4) Travel location *Teacher Location 2 will incur a rental fee to be paid by the student directly to the studio on the day of the lesson. Please inquire for more info! *Teacher Location 2 is also available for anyone wanting a larger dance space or theater. Please ...
Learn to move and improve, gain confidence, get in shape, and have fun doing so! Whether you are a beginner or just looking to refine your existing process and skills, I'm here to help you reach your goals!--It's what I do, and I love it (=
I am a teacher, choreographer, and professional dancer with 14 years of teaching and industry experience both nationally and abroad. I am a versatile artist and educator with a background in numerous styles of dance, modeling, acting, fitness, theater, circus, and character performance. I have been in music videos, commercials, films, and on TV, and have worked with companies like Reebok, Disney, American Idol, Fox, NBC, Heineken, Universal and more. I have traveled the world teaching and performing and I am committed to sharing my knowledge with my students to help them reach their fullest p
I have worked with preschoolers through adults of all ages ranging in all levels, including beginners! My lessons are engaging, creative, beneficial, and a super great time! I enjoy helping my students reach their goals and beyond while having fun along the way. My favorite part about teaching is seeing students improve in ability and more importantly, confidence, and knowing that I was able to be a part of their process.
Lessons and offerings: -Dance fitness -Barre/Cardio Barre -Strength and conditioning -Stretching and Flexibility training -Personal training -Nutrition and wellness -Jazz dance -Hip hop/urban dance -Jazz funk dance -Burlesque dance -Contemporary dance -Lyrical dance -Tap dance -Ballet dance -Musical theater dance -Character development -Acting -Improv -Choreography -Wedding dance -Modeling and much more! Have a question about lessons? Just ask! (:
My lessons focus on inspiring creativity, encouraging self-confidence, and cultivating personal achievement in everyone I work with. I am outgoing, friendly, fun, and I love to teach!
Lesson locations: 1) Online: via TakeLessons Classroom 2) In Studio: 2 locations *addt'l. rental fee req'd-please inquire for more info. 3) Your location
Semi-private and group lessons available *Please inquire for more info!
I look forward to helping you grow!
(Bio Continued) I have appeared on Parks and Recreation, The Disney Channel's So Random, Bring It On: Fight to the Finish, Fox Atomic’s The Comebacks, TruTV's Fake Off, and The Dance Network's Steady Mobbin’, and commercials for Tresemme, eHarmony, El Jimador, and HTV Network, amongst others. I have been in music videos for Cody Simpson, Bassnectar, Claude VonStroke and Bruno Furlan, Beats Antique, Lafa Taylor, Nash Overstreet, Japanese icon Namie Amuro, Ray Quinn from Britain’s Idol, and more.
I have danced in Michelle Obama's “Let’s Move Burbank” Campaign, The Giffoni Film Festival, The Probowl, The Orange County Film Festival, KROQ Radio, Coachella Music Festival, The Wallis Annenberg Center for the Performing Arts, The American Cancer Society, Bettis Richardson on his international tour, and popular rock band Lifehouse.
I am a former member of Unity LA, under the direction of Dancing with the Stars Emmy Winner, Tessandra Chavez, as well as the non-profit dance company, MusEffect- dedicated to raising awareness of social issues through performance. I have collaborated with distinguished circus troupes such as The Lucent Dossier Experience, Zen Arts, Cirque Berzerk, and others, with whom I continue to perform internationally.
Want to dance like the pros? Interested in learning the latest dance trends and develop your own unique dance style? I will help you build a solid foundation of dance theory and technique while tailoring to your personal tastes and goals. Part of my lesson plan involves me breaking down the basic movements of dance in a way that allows you an easy learning experience to build on further dance development to reach your full potential as a dancer. All ages are welcome. Shoot me a message to see if we're a good match!
I am a professional Dancer and Choreographer. I graduated from the University of Alabama with a Bachelors in Kinesiology.
Dance students can expect to improve week to week in their Dance technique. Every dance has a unique rhythm; I teach students to release built up tension and apply foundational techniques in order to unlock their own individual style. I can help any student to dance with freedom, confidence, and expression.
I teach students of all different ages and experience levels. I studied hip hop dance for 11 years. I can teach students to learn their bodies natural flow and to also improve their balance.
As a professional dancer I frequently take trips to New York for video shoots, recording artist, and meeting with other dancers for group dance projects. I have dance for Nike the last two years which as been an amazing exper
ience which also taught me so much and makes me appreciate what I do even more. I love to mentor students who want to become effective, dynamic dancers and reach their full potential to take their dancing to the next level.
My personal passion is dance but I firmly believe that great technique is essential to any and every style. I look forward to meeting you and helping you to become a better dancer!
Experienced teacher of 7 years specialized in beginner, intermediate and advanced dance training. Dance lessons with me would be impactful. With the gift of dancing that I exhibits not only with performing but also to inspire others to pursue their dreams is what I hopes my students gain from each lesson. I continue to inspire the youth to go after their dreams no matter what occupation it may be. A quote that I advocate wholeheartedly is “To never get discouraged but encourage others to find something that they are passionate about and go for it”.
Experienced teacher of 7 years specialized in beginner, intermediate and advanced dance training. After each lesson , I want my students to feel inspired and capable to accomplish their dreams. A quote that I advocate wholeheartedly is “To never get discouraged but encourage others to find something that they are passionate about and go for it”.
I like to make dancing fun and easy. Anyone can dance! Also if you plan on dancing socially I like to help prepare you for all social dance settings so no matter what you will confidant and prepared!
Dancing has always been a passion of mine and I love to share that passion! I love teaching dance any level and skill. Are you ready to take the next step? If you want to dance socially or just for yourself, be better on stage or for any reason, I can help you get to your goals! If you have any questions please let me know!
I have earned my degree in Dance Performance by studying Ballet, Modern, Tap, Jazz, Choreography, etc. As a person who has been insecure about my body, I was able to accomplish believing in myself and working on my creativity. I want to spread confidence to those who believe in themselves and want to grow through dance as a career or even if it is just for fun.
Hello! I’m Ronesha Butler and I just graduated from the University of Arkansas at Little Rock with my Bachelor’s of Fine Arts Degree in Dance Performance. (Spring 2019). I also just got back from a month trip in Tuscany, Italy dancing at the Accademia dell’Arte! I love dancing ballet, modern, contemporary, lyrical, jazz, majorette, praise dance, and some hip-hop. I would love to have more teaching experience and spread the knowledge that I have gained throughout the years for my love and passion of dance.
I have over 15 years experience of Hip Hop and African Dance. I was chosen to dance in Janet Jackson's Escapade video and I also have done commercials doing Hip Hop and African dance. I was chosen to be the principal dancer in the dance commercials I was recently in. Dance is a great form of expression that brings joy to the dancer and audience. I would love to teach you to learn Hip Hop and African dance as it is a fun & joyous expression of dance.
I have danced over 15 years doing Hip Hop and African dance. I auditioned and was chosen to be in Janet Jackson's Escapade video as background dancer. I have been chosen to be in commercials as the principal dancer doing Hip hop and African dance. Dance is a great expression of joy, I love Hip hop and African dance because it is a dance that you can do at a party or at home. I would like to teach you to dance to your favorite Hip Hop songs and African drumbeats in my dance class.
I have been dancing since I was 8. I specialize in modern, jazz, ballet, hip hop, reggae, latin. Dancing is a passion of mine and I would like to share my passion with you.
Good day! I am excited to meet you and share with you my passion for dancing and stretching. Dance is a great form of exercise, it is a great way to keep you in shape without going to the gym 🙂. It is another way to destress when we need a pick me up 🙂.


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“I’ve just been in acting class, which is going awesome and staying busy,” Mirchoff tells The Hollywood Reporter. “You know, I’ve been doing a lot of outdoors stuff. I’ve been doing a lot of scuba diving on the weekends, and hiking, and playing music, and just, you know, hanging out really.”
In fact, he was traveling when the news that MTV had given the series another season broke. “We’ve been done for a few months,” Mirchoff says.
“I was actually flying home from Miami,” he remembers. “And just about to take off, and I got a text from my manager saying, ‘We got a Season 2.’ So, yeah, it was an exciting plane ride.”
Viewers have seen Mirchoff play a heart-stealing teen before on ABC’s Desperate Housewives. He played Angie’s (Drea de Matteo) son whose romance with Gaby’s (Eva Longoria) niece leads to a cross country chase on Season 6.
The 22-year-old has also appeared in a recurring role on the Canadian drama, Heartland, and his film credits include I Am Number Four, The Grudge 3, and Scary Movie 4.
THR spoke to Mirchoff about what we can expect from Matty and Jenna’s romance this season, where he’d like to go with his character in Season 2, and what he has in common with the popular Matty on the MTV hit.
The Hollywood Reporter: Where would you like Matty’s story to go in Season 2?
Beau Mirchoff: I think just to dive more in depth into Matty’s life to see what makes Matty the way he is and what he loves, and his aspirations, and just more details about Matty. I think that’s where I have the most fun is when it’s emotional stuff or just high stakes and I find that’s when it’s the most fun, and more enjoyable – when you really get to learn about someone’s character. I think it’s captivating to watch and yeah, that’s what I’m looking forward to, hopefully.
THR: Have you been able to speak with creator Lauren Iungerich about Season 2?
Mirchoff: We haven’t quite yet. You know, we just found out about it. Last year, we got to go into the writer’s room and talk about our strengths and weaknesses. You know, how we would like to see our characters portrayed, and a bunch of stuff and so I’m sure we’ll be going to do that again.
THR: What can we expect from the Matt-Jenna relationship in the remaining episodes this season?
Mirchoff: Kind of like this cat and mouse game. I’ll kind of finally redeem myself, and then something will happen, and it all falls apart. Then, you’re kind of on my side, and then you’re kind of on her side. Then, you’re not on her side; you’re back on my side. I think we’re just so young… stupid kids to be honest. They’re just really kind of naïve, and they don’t know how to deal with relationships, and communicate, really. That’s the big part, so I think we’re just, throughout the season, we’re trying so desperately to be with one another, but it just doesn’t work out, but it might work out at the end.
THR: What would you say you have in common with Matty and what makes you different from him?
Mirchoff: I think a lot of me is Matty. You know, outgoing like Matty. I’m athletic, I like girls; those are some similarities. I don’t think I’m as self-conscious as Matty — like he is kind of figuring everything out internally. He’s still cautious. He’s afraid of what other people think of him. He wants to be perfect. He has that feeling of wanting him to be perfect, and his family to be perfect. But he doesn’t want anyone to know that, so he’s kind of secretive, in that sense. Later in the season, he keeps some secrets from Jake [Brett Davern], his best friend and you would think he would tell his best friend anything, but he doesn’t because he has this wall up. Me though, in real life, I’m not really like that. I’m pretty easy going, I think. I think it’s just that I’m older than Matty, but you know, we’re very similar, we really are.
Awkward airs Tuesdays at 11 p.m. on MTV.
Email: firstname.lastname@example.org; Twitter: @TheRealJethro
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text:
Hair transplant refers to the operative procedure that entails the removal of hair from the legs and palms. The plantation of the hairs are at the hairless region on the head. It is among the latest systems readily available for the management of hair thinning troubles. The haartransplantation münchen specialists can provide the solution as per the necessity and condition of women and men. You can find alternative methods offered, but you should prp munich choose the best types.
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domain: biology


text:
The proper place for psychiatry education and experience in the medical school curriculum has evolved over time. Some institutions have limited the time spent on the required third-year psychiatry clinical clerkship to 4 weeks. Most institutions offer a 6-week clerkship, but there have been questions as to whether maintaining this level of experience is viable, given additional curriculum demands (1).
Within the clerkship itself, change is “in the air.” A traditional experience at many schools has been a clerkship spent entirely at an inpatient facility. Increasingly, there is a call for greater integration of psychiatry and other medical specialties, in addition to clinic-based and longitudinal experiences during clinical clerkships. Diversification of clerkship sites has resulted in opportunities for other models to compete with the familiar inpatient rotation, with similar educational value (2).
Inpatient psychosomatic medicine (PSM) offers several advantages for the third-year clerkship. The patients are admitted to the medical center, so that the third-year clerks experience psychiatric illness in the context of acute and/or chronic medical illness, operationalizing the biopsychosocial model (3–7). Students on PSM services manage several streams of clinical data (including laboratory values and neuroimaging results) and communications from nursing staff and other health professional disciplines. They must interact with physicians from other medical and surgical specialties, receive consultations, and provide recommendations. PSM services regularly manage cases of delirium and dementia, illnesses that are critically important in the general-hospital population (8).
Perhaps most important for third-year students is the opportunity to experience the specialty of psychiatry being practiced in a medical model, co-located with other specialty colleagues, in a collaborative environment. One commonly sees students who possess the empathic and intellectual gifts to become psychiatry residents, yet hesitate to consider the specialty for fear of “not being a real physician anymore.” These students may experience PSM as a model where one can continue to be fully integrated into the medical and surgical environment while still being able to utilize psychiatric assessment and psychotherapy skills. Also, the PSM setting provides a clear example of clinical practice from a biopsychosocial model consistent with the framework taught to students in other specialties.
The University of California, Davis School of Medicine (UCDSOM) has included the PSM service at its major teaching hospital, University of California, Davis Medical Center (UCDMC) among its third-year clinical offerings for over 20 years. UCDSOM requires an 8-week clerkship in psychiatry. The majority of students spend 4 weeks at two different sites. During the last 5 academic years, approximately 25% of third-year students have completed 4 weeks on the PSM service. While on the PSM clerkship, students worked with two PSM faculty psychiatrists, one clinical nurse-specialist, one PG-4 psychiatry resident, and two PG-1 psychiatry residents. The PSM service also trains off-service rotating physicians from family medicine, internal medicine, and neurology. Students were responsible for initial chart review, initial patient interview, integration of laboratory and neuroimaging data, case formulation, treatment planning, and follow-up care, all under supervision of residents and/or faculty members. The PSM service was the only consultation service in the required clerkships in the third year. The two PSM faculty psychiatrists on the service were assigned year-round as their primary clinical service responsibility; these two PSM faculty members also had clinic responsibility for outpatient PSM services in organ transplantation and gastric-bypass surgery.
The authors undertook this study to characterize third-year students’ reflections on the PSM rotation, looking for comparisons and contrasts with other psychiatry clerkship sites at UCDSOM. Specifically they sought to ask whether the data would support the premise that the PSM service offered an integrated experience consistent with the biopsychosocial model. Also, by surveying psychiatry residents at the same institution who had also completed the third-year PSM clerkship, they qualitatively examined the influence that experience as a third-year PSM student might have had on these residents’ motivation to pursue a residency in psychiatry.
This study was approved by the UCDSOM IRB as an “Exempt” study. For the period 2004–09, the 9-item Performance Analysis routinely gathered at the end of MS-3 clerkships was examined. This is a Likert-type scale, where educational goal attainment is rated in response to declarative statements on a scale of 1: Strongly Disagree to 7: Strongly Agree with positive assertions about the educational experience. Also, students’ free-text responses (Site Comments) on their reflections on the PSM clerkship were examined. In addition, a small cohort of physicians who were currently in the UCDSOM psychiatry residency program who had also completed their M.D. degrees at UCDSOM during this period were asked to reflect on how their experience on PSM as a third-year clerk may have affected their motivation to pursue postgraduate training in psychiatry.
On quantitative analysis of clerkship experiences, the students’ ratings of the PSM site paralleled the high ratings for the clerkship overall. On the 9 items, the mean score was 6.0, with a range of 5.3 to 6.3 for the PSM service, with the mean score of 6.2 and a range of 5.8 to 6.4 for all sites. An analysis of the written comments from students about PSM as a clerkship site revealed numerous comments reflecting the students’ appreciation of the specific content of the PSM service. These themes and a number of related comments included 1: Diversity of patient population beyond “pure psychiatric patients” (12 comments); 2: Integration with medicine and application of the biopsychosocial model (7 comments); 3: Application to clinical experience outside of psychiatry (6 comments); 4: Interaction with medical colleagues in other specialties and exposure to an acute-care medical hospital setting (6 comments); and 5: Unique exposure to patients with delirium, dementia, traumatic brain injury, and making decisional-capacity determinations, etc. (6 comments). The reflections of four of the seven psychiatry residents surveyed were similar to those of the students, and typically included an appreciation of the benefit of the integration of psychiatry and medicine and experience in the application of the biopsychosocial model afforded them on the PSM service as students, which was felt to be an important experience in their eventual choice to pursue postgraduate training in psychiatry.
This study covered a 5-year period, during which 25% of UCSDOM students completed a PSM rotation as part of their MS3 core clerkship. The student ratings for the PSM site numerically paralleled the high ratings for the clerkship itself. The free-text comments show that students appreciated the unique aspects of a PSM rotation for their MS3 experience in clinical psychiatry, with themes consistent with our hypothesis.
Perhaps due in part to the success of this clerkship site, the institution where this study was completed had a high rate of students matching into psychiatry residency programs from 2005 to 2009 (range: 6.0%–14.6%; median: 12.0%). The psychiatry residents who had received their M.D. at the same institution had several comments regarding the effect of their experience in PSM on their motivation for psychiatry residency. The comments from students and residents also suggest a uniquely useful role for PSM in assisting those students struggling with the issue of “giving up medicine” in choosing psychiatry.
PSM, a model that, by definition, functions on the medical–psychiatric interface, offers a specifically illustrative experience for third-year students. When students are first exposed to psychiatry, in a purely psychiatric setting, there is always a risk that they will overgeneralize from these experiences and not fully appreciate the significant diversity in patient-care models in the specialty. PSM, by virtue of its medical/psychiatric orientation, presents psychiatry in an integrated model with the rest of medicine. Students on PSM services regularly encounter physicians from many other specialties, who are also working with these patients. Students on PSM services regularly experience important problems such as drug interactions and the systemic effects of psychotropic medication. Given this, one can argue that a medical student’s experience on PSM may be one of the most valuable psychiatry rotations, especially for the 95% of students at most medical schools who will pursue postgraduate training in other specialties.
For the development of a robust MS3 clerkship in PSM, several attributes may be desirable. A PSM service benefits from a viable clinical “load” of patients, so as to ensure adequate and clinically diverse experience. A stable clinical faculty cohort with a primary clinical and academic identity in PSM (rather than coverage by a series of “rotating” faculty) provides for essential supervisory continuity and clinical currency in clinical practice and mastery of the PSM literature. “Building in” of specific PSM content into the pre-clerkship psychiatry curriculum (as is done at UCDSOM) prepares students for clinical encounters in PSM. Integration of student PSM experience with an established and robust resident experience is most beneficial. Finally, training on a PSM service with the regular participation of residents from other clinical departments allows for additional clinical perspective on the cases seen.
Although there are many clear advantages to the PSM site for medical-student clerks, there are possible disadvantages to consider, including the loss of the potential de-stigmatizing effect of working in a traditional psychiatric hospital, with its exposure to actively psychotic patients who cannot be “talked out” of their illness. The PSM site also requires closer supervision of medical students than for the psychiatry residents on these services.
Strengths of this study included a 5-year sampling with good data capture, the benefit of reviewing de-identified data, which encourages frankness in commentary, and the fact that the PSM educational model was under stable leadership and structure throughout the period of the study. Weaknesses of the study involve a retrospective and primarily qualitative design, a lack of data from a period where there was no third-year PSM rotation, and an incomplete response rate on the resident survey.
Schools of medicine and departments of psychiatry should actively consider having a third-year clerkship in PSM routinely available. The clinical experience can be seen by students as widely applicable to a range of specialties. The opportunity to experience and study medical/psychiatric integration on a PSM service is unique to this model. A well-run PSM service, in the context of an educationally strong and student-centered department, can be a significant site for the favorable presentation of psychiatry, and may meaningfully contribute to the recruitment of appropriate students into the specialty.

domain: medical


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A Collection of Thoughts, Poetry & Praise
So true! But it's still a cute picture ;)
To barrow a quote from another holiday, "let's remember the reason for the season."Mervi
Very true, but the photo is adorable. My dog would never sit still long enough for me to take a picture of her wearing bunny ears. LOL
That is so cute!!! Great match for the statement!!!
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The neurologist, immediately after giving her a two-moment exam, instructed tests that had currently been done and wrote a prescription that he admitted was of Uncertain profit. Luz’s spouse and children appeared to come across this kind of disorganization, imprecision, and waste wherever his mom went for aid.
I viewed Mauricio Gaviria at the broiler station since the lunch group started coming in. Mauricio was 20-9 several years aged and experienced worked there 8 a long time. He’d acquired his get started executing basic prep—chopping veggies—and labored his way nearly fry Cook dinner, the pasta station, and now the sauté and broiler stations. He bounced in place awaiting the rate to pick up. An buy for your “hibachi” steak popped up.
A mindful examination is fabricated from how the bordering forces of legislation, regulation, and market activity affect the standard of treatment provided by overall health treatment companies after which seems to be at their handling of health care issues.
Armin Ernst has huge programs to the command Centre—a rollout of full-scale treatment method protocols for sufferers with intense sepsis, acute respiratory-distress syndrome, along with other ailments; tactics to reduce pointless expenses; maybe even computer forecasting of affected individual volume sometime.
Incorporating psychodynamic, cognitive behavioral therapy (CBT), mindfulness and psychodramatic tactics, he has helped his shoppers decrease TMS/PPD indicators, additional masterfully deal with challenging feelings, and even more skillfully cope with lifestyle’s adversity. At the moment, Andrew is effective in non-public apply and runs teams at therapy facilities throughout La.
A few days immediately after her operation, she was acquiring in and off the bed on her have. She was on practically no narcotic medication. She was starting to climb stairs. Her knee agony was actually under prior to her operation. She remaining the hospital to the rehabilitation Middle that afternoon.
I spoke to David Gordon, the organization’s Main operating officer. He explained to me the Cheesecake Manufacturing unit has labored out a personnel-to-customer ratio that keeps everyone active although not so hectic that there’s no slack during the system from the function of a sudden surge of shoppers. Harder is the situation of squandered meals. Although the corporation buys in bulk from regional suppliers, groceries are the most significant expenditure following labor, and the most unpredictable.
I puzzled how they pulled it off. I questioned one of many Cheesecake more tips here Manufacturing facility line cooks exactly how much in the foodstuff was premade. He instructed me that every thing’s essentially made from scratch—other than the cheesecake, which essentially is from the cheesecake manufacturing unit, in Calabasas, California.
Although fewer than 1 in 4 thousand Us residents are in intense care at any offered time, they account for four for every cent of national health-care expenses. Ernst thinks that his occupation is to make sure that everyone seems to be collaborating to supply the simplest and least wasteful treatment attainable.
Dr. Barta is actually a licensed psychologist. She claims "I have a person non-public follow And that i take pleasure in dealing with shoppers diagnosed with PPD / TMS. I frequently call for that any remarkable physical disorders are ruled out 1st by a medical doctor. Consulting a clinical medical doctor who treats or understands PPD / TMS may be warranted, but usually the shopper can consult with any medical professional in their selection.
I comply with my information and facts becoming processed by TechTarget and its Partners to contact me through mobile phone, e-mail, or other implies relating to details appropriate to my Specialist interests. I could unsubscribe Anytime.
In March, my mom underwent a total knee substitution, like no less than six hundred thousand People each and every year. She’d had a partial knee substitution a decade back, when arthritis had worn away Element of the cartilage, and for some time this served her beautifully.
Ernst wasn’t oblivious of the issue. He had taken pains to introduce the command center’s group, expending months going to the units and bringing Medical practitioners and nurses out to tour the tele-I.
“We now have forecasting designs determined by historic info—the development on the earlier six months and also the craze of the former yr,” Gordon explained to me.


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Four leaves with four miniatures from an illustrated manuscript of Hafiz-i Abru's Majma al-Tawarikh (The Assembly of History), depicting the devil Shakymuni in disguise; the reign of Bazdjurud bin Bahram; the caliphate of al-Qa'im bi-Amrallah; and an audience scene
Timurid Persia, Herat, circa 1425
Persian manuscript on paper, 33 lines to the page written in clear naskhi script in black ink, significant words and numerals picked out in red ink, inner margins ruled in red and blue, some repairs, slight staining mostly restricted to outer borders
430 x 330 mm.(4)
- The court scene forming part of lot 7 (illustrated top left in the catalogue) was in the 1936 New York auction of the Tabbagh Collection, lot 222 (illustrated), where it is identified as Al-Kaim ibn-Amrillah Enthroned. It has been suggested that the other paintings were in the same sale, but are not illustrated. Several of the descriptions in the 1936 catalogue resemble the paintings of a seated ruler with a sage in a wide-brimmed hat seated on a bench in front of him, as seen in lots 7 and 8.

domain: artistic


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Following Ravens' formula, Bengals becoming annual threat in AFC North
Marvin Lewis was the Ravens' defensive coordinator in 1996 when the franchise set its foundation with the drafting of offensive tackle Jonathan Ogden and middle linebacker Ray Lewis.
By Jeff Zrebiec, The Baltimore Sun
November 10, 2013


A city study on trees released Monday provided a glimpse of what can happen to Charlotte’s beauty and health when city officials wilt at the will of developers.
The report was done at the request of the Charlotte City Council, which last year adopted a goal to have a 50 percent tree canopy in the city by 2050. Right now, we’re at about 46 percent, but we’re continuing to lose trees to age, weather and, of course, development.
The latter isn’t a bad thing in itself, but when Charlotte grows without thought to its environment, the city suffers in several ways. One is the loss of trees, which provide buffering and filtering for storm waters. They also offer shade while removing carbon dioxide and reducing ozone levels. Plus, they make our city a prettier place.
So here’s the bad news: About 10,000 new trees are planted each year in Charlotte, which is 15,000 short of the annual pace needed to reach the 50 percent tree canopy goal. Monday’s report showed a big reason we’re behind in the first place – a developer-friendly tree ordinance that allowed builders to essentially clear-cut healthy trees instead of smartly letting them coexist with single-family homes. The city’s report included a dozen non-uptown neighborhoods larger than 25 acres that had a tree canopy of less than 10 percent. If that doesn’t seem like many, keep in mind that city staff recommends neighborhoods having a canopy of 55 to 60 percent. The neighborhoods in the report were merely the worst of the worst.
The council has taken baby steps toward improving our collective shade; in September 2010, the city toughened its 1978 tree ordinance, which now requires a 10 percent tree canopy in new single-family housing developments, and 15 percent saved canopy in commercial development. That’s still far from recommended levels, and the council has been weak-kneed about enforcing even that, sometimes allowing developers to buy their way out of saving trees. And last October, Mayor Anthony Foxx had to wave a veto in front of the council to get it to reconsider loosening tree rules for developers in some subdivisions.
Monday’s report, however, also showed how Charlotte might be turning a corner toward leafiness. The city is working out details on a matching grant program that would help neighborhoods pay for the cost of planting trees, and a public-private collaboration is making serious fundraising and strategic inroads to address the tree canopy issue.
That effort, TreesCharlotte, is led in part by a Who’s Who of city leaders, including chair Marcia Simon, Dave Cable, Betty Chafin Rash and former Observer publisher Rolfe Neill. TreesCharlotte has partnered with the city and Foundation for the Carolinas, and it has begun reaching out to businesses that want to donate money and get employees involved in planting trees. The goal: support the planting of the extra 15,000 trees needed annually to help reach the 50 percent canopy goal.
Charlotteans should embrace this worthy effort, and council members can do their part, too – not only by crafting a smart, efficient matching grant program, but by remaining committed to our canopy the next time a developer wants to take a bulldozer to the rules.


Music composition software allows you to create original compositions via your Mac or Windows PC or laptop. You no longer have to painstakingly write out a song sheet by hand – and then photocopy it to share it with other people.
A good music composer program should provide you with all the tools you need to compose a simple or highly sophisticated score. It should have a user-friendly interface. It should be flexible in terms of how notes can be inputted. You can use it to notate your lead sheet, guitar tab, band chart or orchestral scores.
There are many good music creation programs you can turn to. Here are three top-rated composer softwares for PCs. Each will help you realize your inner Mozart or Beethoven. They all have a user-friendly interface.
They come with affordable prices. Their functionality will suit the needs of novice, intermediate, and expert musicians. You will be able to easily input your notes and judge your progress accurately via a realistic playback mechanism:
Top-Rated Music Composer Programs
Finale Music Composer Software
Finale has been in business for nearly three decades. Its music notation software is often acknowledged as the industry standard.
It has a reputation for making the best value-for-money softwares. It offers a very flexible interface that you can use to adjust measures, notes, key signatures, and other score elements.
- Flexible note entry. You can input notes a MIDI keyboard, mouse or computer keyboard. (You will need a microphone to record vocal performances.) You can also scan existing sheet music and import various file types.
- Good sound library. It includes orchestral effects and instruments from four Garritan libraries. This includes the Garritan Personal Orchestra, World Instruments, Jazz & Big Band and Instant Orchestra.
- Additional features. The 2014 edition comes with several innovative features. This includes Linked Parts, Music Educator Resources, Smart Shapes, Band-in-a-Box Auto-Harmonizing, MicNotator, Video Support, and Tempo Tap.
- Functional updates. This edition will please longtime users with its various core improvements. It has a new audio engine, Macintosh Cocoa support, percussion enhancements, and minimalist new interface. The latter includes updated palettes, playback, controls, mixer, icons, and backgrounds.
- Ease of use. The 2014 edition will be user-friendly to existing Finale users. It will also be hospitable to first-timers.
- No 64-bit support. Many professional sample libraries require 4 GB RAM or more. (These libraries feature sound samples from large orchestras) This requirement can only be met by 64-bit applications.
- Affordability. The academic edition can be purchased at under $300 from Amazon. This makes it the most expensive high-quality composer app for PCs in this review.
This is a time-tested, user-friendly, and well-received recording software. It will be suitable for most music teachers and composers. It also offers a solid starting point for beginners.
Sibelius First 7
The Sibelius First 7 describes itself as the “fastest, easiest way to write and share music”. This app certainly stands as one of the best music composer software options in the market.
The Sibelius First series is a more streamlined version of the full-featured Sibelius 7 (which costs over $500). It should satisfy the needs of nearly every music maker, from aspiring to professional songwriters and composers. It is compatible with Mac and PC users.
- Flexible note entry. You can add in notes via a MIDI keyboard, a mouse, a computer keyboard, or the virtual piano. You can also import files via MusicXML interchange, transcribe audio (AudioScore), or scan existing printed sheet music (PhotoScore).
- Affordability. The Sibelius First 7 can be purchased at under $130 from Amazon.
- Beginner-friendly. It comes with Score Starter templates that will keep your ideas flowing in several genres: rock, classical, funk, jazz, pop, reggae, Latin, country, choir, and blues.
- Ease of use. The full-sized virtual piano keyboard makes music composition breezy. The copy and paste functions allow you to easily modify multiple notes at once. The Sibelius First 7 also allows you to communicate with your bandmates or co-songwriters via virtual sticky notes.
- 64-bit capacity. This is a 64-bit application that will allow you to access over 4 GB of RAM. You can work with the full repertoire of professional sample libraries.
- Additional features. The Sibelius First 7 is equipped with time-saving tools like Magnetic Layout.
- Ease of sharing. Once you are satisfied with your composition, you can easily share your score with the world by exporting it to your iPad. You can also share it directly to YouTube, SoundCloud, and Facebook.
- Not versatile. This song composer software cannot export scores as graphics files or MusicXML.
- Storage requirements. This music composer app for PCs will occupy approximately 500MB of your hard disk space.
This is a great music notation software as long as you intend to create your compositions within the framework of 16 staves. It is affordable and user-friendly. It accommodates experienced and novice musicians and composers alike.
Finale PrintMusic has all the basic features that you expect from a good music notation program. It comes with guitar chord diagram creation, guitar tabulation, chord names, manual step-time entry, multiple tool palettes, and a volume mixer.
- Versatility. You can enter notes through a keyboard, MIDI, or scanner. You have can your score appear as handwritten or engraved symbols.
- Affordability. This is a real money-saver if you do not need the advanced features found in the full version. You can buy a second-hand version at under $100 from Amazon. Or, you can opt for a new one at under $150. The full version of Finale costs $600. (Do note that the full version can only be operated by Windows computers). This makes the Finale PrintMusic a far more affordable and versatile option. As long as you can notate within a 24 stave framework, it will serve as a good fit. It is compatible with musicians and composers of all experience levels.
- Good sound library. It comes with a free software synthesizer with 128 plus instrument sounds. The Human Playback feature also gives the playback a more human touch.
- Additional features. Scroll View displays your score horizontally. This allows you to see a larger portion of your score at a time. You can work more efficiently without having the scroll down the page so often. Editing features include a volume mixer and multiple tool palettes.
- Ease of use. Users will find a formidable rate of note entry and editing palettes at their fingertips. However, these have been designed to be intuitive and user-friendly. A tooltip appears over each tool when your cursor hovers over it. This time-saving innovation eliminates the need for browsing for answers online. You also do not need to comb through the manual. You can print and export your sheet music as graphics and MIDI files.
- No advanced features. It omits the more advances capacities that you can find in the full version. (E.g. a virtual piano for more precise note placement).
This is a highly affordable and user-friendly music notation software. It fits the purposes of a wide range of composers, teachers, arrangers, students, and amateur to professional musicians.


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Microalgae are a rich source of proteins that have the potential to be utilized in various industries, ranging from food and pharmaceuticals to biotechnology and biofuels. Extracting and purifying protein from microalgae cells is a complex process that requires careful consideration of factors such as cell disruption methods, extraction solvents, and purification techniques. In this article, we will delve into the intricacies of how we can effectively extract and purify protein from microalgae cells to harness their full potential for a wide range of applications.
Optimal Extraction Methods for Protein Isolation from Microalgae Cells
The most effective extraction methods for isolating proteins from microalgae cells include mechanical disruption techniques such as bead beating, sonication, and high-pressure homogenization, which are able to break open the tough cell walls of microalgae to release the protein content. Additionally, enzymatic digestion using proteases can help to further break down the cellular components and release proteins. Finally, solubilization with detergents or organic solvents can aid in separating proteins from other cellular debris. Overall, a combination of these methods is often used to effectively isolate proteins from microalgae cells.
How can we ensure maximum protein yield during the extraction process?
To ensure maximum protein yield during the extraction process, several key factors must be considered. Firstly, selecting the most appropriate extraction method based on the nature of the protein and the sample matrix is crucial. This may include methods such as precipitation, chromatography, or membrane filtration. Additionally, optimizing the extraction conditions such as pH, temperature, and buffer composition can help to improve protein solubility and stability. Ensuring proper disruption of cell walls or membranes through techniques like sonication or grinding can also aid in releasing more proteins for extraction. Finally, careful handling and storage of samples throughout the extraction process can prevent protein degradation and maximize yield. Overall, attention to detail in each step of the extraction process is essential to achieve the highest possible protein yield.
What factors influence the purity of extracted proteins from microalgae cells?
The purity of extracted proteins from microalgae cells can be influenced by several factors, including the efficiency of the extraction process, the presence of contaminants such as lipids and pigments, the choice of extraction method and solvents used, the condition of the cells before extraction (such as cell disruption technique), and the experience and expertise of the researcher performing the extraction. Additionally, the type and species of microalgae being used can also impact the purity of the extracted proteins, as some species may have higher protein content or be more difficult to extract proteins from compared to others. Proper optimization and careful consideration of these factors are crucial in obtaining high-purity protein extracts from microalgae cells.
Is there a way to selectively extract certain types of proteins from microalgae cells?
Yes, there are various methods that can be used to selectively extract certain types of proteins from microalgae cells. One common approach is to use differential solubility techniques such as centrifugation or membrane filtration to separate the proteins based on their physical and chemical properties. Additionally, specific antibodies or ligands can also be used for affinity purification to isolate target proteins. Furthermore, advanced technologies such as chromatography and electrophoresis can be employed to further purify and separate the proteins of interest. Overall, a combination of these methods can enable the selective extraction of specific proteins from microalgae cells for various applications in biotechnology and research.
How can we prevent degradation of proteins during the extraction and purification process?
Proteins can be prone to degradation during the extraction and purification process due to factors such as enzymatic activity, pH changes, and oxidation. To prevent degradation, it is important to work quickly and efficiently, using buffers and additives that stabilize proteins and prevent denaturation. Minimizing exposure to oxygen and light, maintaining proper temperature control, and avoiding repeated freeze-thaw cycles can also help preserve protein integrity. Additionally, gentle handling techniques, such as using sonication or vortexing instead of vigorous mixing, can reduce the risk of damage to sensitive proteins. Regularly monitoring protein quality throughout the extraction and purification process through techniques like SDS-PAGE or Western blotting can also help identify and address any degradation issues early on.
Are there any potential contaminants that could affect the quality of extracted proteins from microalgae cells?
Yes, there are several potential contaminants that could affect the quality of extracted proteins from microalgae cells. These include heavy metals, pesticides, herbicides, and other chemical pollutants that may be present in the water where the microalgae are grown. Additionally, microbial contamination from bacteria, viruses, or fungi could also impact the purity and quality of the extracted proteins. It is important to carefully monitor and control the growth conditions of the microalgae to minimize the presence of these contaminants and ensure the quality of the extracted proteins.
What is the most efficient method for purifying extracted proteins from microalgae cells?
The most efficient method for purifying extracted proteins from microalgae cells is typically a combination of techniques such as cell disruption, centrifugation, and chromatography. Cell disruption methods, such as sonication or homogenization, are used to break open the cells and release the proteins. Centrifugation is then employed to separate the cellular debris from the protein-containing supernatant. Finally, chromatography techniques, such as ion exchange or size exclusion chromatography, can be utilized to further purify the proteins based on their charge, size, or other properties. By combining these various methods, a high level of purity and yield can be achieved for the extracted proteins from microalgae cells.
Are there any new technologies or techniques that could improve the efficiency of protein extraction and purification from microalgae cells?
Yes, there are several new technologies and techniques that could improve the efficiency of protein extraction and purification from microalgae cells. One such technique is the use of high-pressure homogenization, which can disrupt cell walls and release proteins more efficiently than traditional methods. Additionally, advancements in chromatography techniques, such as affinity chromatography and membrane filtration, have been shown to increase the purity and yield of extracted proteins. Furthermore, the development of automated systems and microfluidic devices for protein extraction and purification could further streamline the process and reduce the time and resources required. Overall, these innovations hold promise for enhancing the efficiency and scalability of protein extraction from microalgae cells.
Optimizing Protein Extraction and Purification from Microalgae Cells
In conclusion, extracting and purifying protein from microalgae cells requires a combination of efficient cell disruption methods, such as sonication or bead milling, followed by various separation techniques like centrifugation, filtration, and chromatography. By carefully optimizing these steps and selecting the most suitable purification method for specific proteins, we can effectively isolate high-quality protein from microalgae cells for various applications in food, pharmaceutical, and biotechnological industries. Additionally, advancements in technology and research will continue to improve the efficiency and sustainability of protein extraction and purification processes from microalgae, making them an increasingly valuable and versatile source of protein in the future.

domain: chemistry


When faced with a damaged vehicle, your first thought might be, “I want it looking brand new – replace all the damaged parts!” However, the road to restoring your vehicle to its original glory might not always be as straightforward as simply replacing all the damaged components. Here, we aim to shed some light on the complex decision-making process that goes behind each repair.
When to Repair and When to Replace?
Each vehicle and its damages are unique. Whether a part should be repaired or replaced doesn’t just hinge on the extent of visible damage, but also on some technical aspects that are paramount for your vehicle’s long-term performance and safety.
Protecting Against Future Corrosion: Repairing might involve processes that leave certain parts vulnerable to rust and corrosion in the future. In such cases, replacing becomes a better option.
OEM Procedural Requirements: Original Equipment Manufacturer (OEM) guidelines often specify how parts should be handled after an accident. Sometimes these procedures mandate replacement even if the part seems repairable.
Part Availability: If a specific component is rare or back-ordered, repairing the existing part might be the quicker route to get you back on the road.
Cost Implications: While your primary concern is likely the safety and aesthetics of your vehicle, it’s also worth noting that sometimes repairing can be more cost-effective than replacing, or vice versa.
Open Communication is Key
It’s your vehicle, and you have every right to know and understand the decisions being made about its repair. Always feel free to ask questions. Whether you’re curious about the repair process, warranty, timelines, or cost estimates, we’re here to provide clarity.
At Liberty Collision in Sparks, NV, we believe in a transparent repair process. We recommend you always discuss your preferences and concerns with our professionals. Our team is trained to provide guidance, ensuring the safety and longevity of your vehicle while also catering to your desires and comfort.
Contact Liberty Collision for Collision Repair in Sparks, NV
Remember, it’s not always about repair vs. replace, but rather what’s best for your vehicle’s health, safety, and longevity. Trust in the expertise of collision repair professionals like us at Liberty Collision, and always prioritize open communication.
Keep rolling safely, and know that we’re here for every bump along the way!


Rhino is a phenomenally professional design tool but when it comes to presenting designs to clients or manufacturers, I feel like I'm hacking a presentation together in high school. Are there any Rhino5 developers available to address a few layout and appearance improvements that would make my ability to present much easier?
Hi Gabe- please post your requests here, on the Rhino news group, or to me directly (email@example.com)
The biggest problem or fix request I have is that we can't hide seams on objects in 'Shaded' views. In the settings they are currently tied with Surface Edges.
Providing two settings that toggled the view of Surface Edges and Seams independently, would solve the problems I have when creating shop drawings with Rhino.
The reason for this request:
Showing hard edges and silhouette edges are very important for clearly communicating an idea to clients and manufacturers. Also the shading information offered in a 'Shaded' view efficiently eliminates any illusions that simple line drawings sometimes create, and especially for clients it communicates design intent effectively. However, the presence of seams between tangential surfaces in drawings always confuse manufacturers and clients -- that's a continual source of frustration.
Seams are fantastic in a design work flow -- they are meaningful and useful. They don't belong in presentation views where they are deceiving to anyone who isn't a CAD designer.
Unless I waste time photoshopping away tangential seams from shop drawings produced in Rhino, I find myself having to make excuses about my design software in an attempt to explain why those lines are there — which is unprofessional.
The 'Shaded' display setting is amazingly efficient, both in computational speed and for design communication. It just needs that one fix -- hide seams, not silhouettes or hard edges.
our VSR Realtime Renderer plug-in has been developed to adress this. E.g. the variant manager is a great tool for presentations. Check here to see some of its features:


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Much like Winter itself, Sunday afternoons during the baseball offseason can be barren, frigid, and devoid of joy and meaning. When baseball usually comes out to play, it is instead holed up inside, trapped in a blizzard of heavily debated projections and player awards, with only small seasonal joys like the GM meetings to break up the monetany. Much like Spring itself, Sunday afternoons during the baseball preseason can be fresh, welcoming, and full of newborn expectations and sights. It makes us both excited for the long sustained life of baseball to come as well as reminds us of what made us love the sport so much in the first place. This is the first Sunday afternoon baseball game of many to come with a long Summer ahead, and it should certainly be a cause for celebration until the Autumn of our days (and beyond).
It is a beautiful day in Peoria, the home of the Padres during Spring Training. It is expected to be partly-cloudy and in the high 70’s, low 80’s, absolutely perfect weather to play a (mostly) meaningless game of baseball, but baseball nevertheless. For what it's worth, the visiting A’s are currently 1-2-1 with both losses coming on the road, and the Padres are 1-3 and playing a split squad game today, meaning they will only be at half strength as the rest of their team will be taking on the White Sox. Chris Bassitt will be making his first start of the Spring for the A’s, and opposing him will be James Shields.
Oakland’s Tentative Starting Lineup is: 1- Burns (8), 2- Semien (6), 3- Coghlan (9), 4- Davis (7), 5- Alonso (3), 6- Lambo (DH), 7- Nunez (5), 8- Wendle (4), 9- Maxwell (2)
San Diego’s Tentative Starting Lineup is: 1- Jay (8), 2- Spangenberg (4), 3- Kemp (9), 4- Solarte (5), 5- Norris (2), 6- Wallace (3), 7- Margot (7), 8- Bethancourt (DH), 9- Rosales (6)
Similarly to the A’s, the San Diego Padres underwent a fairly massive roster reconstruction the offseason before this one. The expectation was that the boring, not-good not-awful Padre teams of the last decade would be a thing of the past and that the Padres could force open a new window of contention under new General Manager AJ Preller, with moves such as adding Matt Kemp, Justin Upton, and Craig Kimbrel. However, similarly to the A’s , the Padres were conspicuous underachievers as they toiled through another season of unexciting mediocrity in 2015. However, whereas the A’s have more or less placed themselves in a position where the team may be few pieces away from contention, but still has the potential for significant growth, the Padres find themselves in a more in flux and transitive state.
This is a Spring Training game, so ultimately the key to the game is keeping everyone healthy, however, there are a few other important factors to keep an eye on for both teams.
For the A’s, look for Chris Bassitt to throw a lot of strikes. Bassitt’s journey has been more than well documented here, particularly by Alex Hall and Tim Eckert-Fong. When the A’s acquired Bassitt, it appeared that he couldn’t succeed as a starting pitcher because of his inability to consistently retire left-handed hitters, but due to a few different adjustments in his approach to left-handers and a better ability to suppress walks, Bassitt surprised most everyone with stretches of dominance as a starting pitcher last season. This offseason, Bassitt has been working out with everyone’s favorite oxymoron Sonny Gray, and has changed his delivery to resemble something of a permanent simple stretch, as opposed to his semi-jerky motion from last season, in an attempt to be more consistent and throw more strikes during the next season. Bassitt somewhat lived and died by his ability to not walk hitters last year, and if he could limit his free passes next season, it wouldn’t be unreasonable to believe Bassitt could take another step forward in his development.
On a more minor note, the A’s are unsurprisingly continuing to take their time with injured players like Stephen Vogt, who DH’d yesterday, as well as Mark Canha, so this is the best time to see what most of the youngest prospects can do before they are shipped off to minor league camp to make space for starting players. The young guys had a bit of an off day yesterday, but overall have been showing very encouraging signs in the early days of Spring Training.
For the Padres, Gaslamp Ball does a nice job outlining the most important factors of the Padres’ spring training, most notably the lack of certainty surrounding 2nd base, Left and Center field, backup catcher, the backend of the rotation, and nearly the entire bullpen. It is at these positions that players have the most to prove, and will undoubtedly be under the most scrutiny from the Padres’ side of the bench.
Old Friends: Drew Pomeranz, Derek Norris, Tyson Ross, Brett Wallace, Jose Torres, and oh so very briefly, Jabari Blash, are all former A’s who are now on the Padres’ 40-Man roster. Adam Rosales is a non-roster invitee.
This is just Spring Training, so now moreso than ever, winning or losing is fairly secondary as opposed to how the team wins or loses. I’m mostly just elated the godforsaken Winter is finally over. Go A's!

domain: hobby


Since it’s inception in 2004, The Magenta Foundation has stood as a pioneer for the continued development of programs and events that promote and foster emerging artists. The arts non-profit will bring its traveling photography exhibition, Flash Forward Festival, of emerging artists from Canada, the UK and the US to Boston for the event’s fourth installment.
Starting Thursday, May 1st photographers, curators, collectors, and photography fans alike will come together for the four-day festival that includes discussion panels, lectures, and several exhibitions showcasing some of the most promising international photographers. Flash Forward Festival brings internationally respected industry professionals, like The New York Times Magazine’s photo editor, Stacey Baker, and local emerging photographers together to share their knowledge about the art form. Programming includes curated indoor and outdoor exhibitions, and galleries throughout Boston.
The festival runs through May 4th at the Fairmont Battery Wharf Hotel in Boston, but several events have already opened and are on display all over the city. For more information about exhibit locations and details, go to Flash Forward’s website.
[This post is sponsored by The Magenta Foundation]
[Photos via Flash Forward website]


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domain: design


text:
My final evening in Antigua is always such a melancholy experience - I hate it when the light starts to seep away and I have to come to terms with the fact that I have yet again used up my precious supply of luminous, birdy-trilled mornings.
On Friday the twilight found us in the market stepping between the candles the last few sellers use to illuminate their produce. On previous final-night occasions we have made for Lloyds' (now Cuzcatlan) corner in the parque − our corner − as the old bank's steps are ideally placed to absorb and retain the warmth of the sun from early in the morning until the time it drops behind Fuego.
I have reorganised the sets of images I took in Central America this Christmas to make them a bit easier to review.
New Year's Eve in Antigua was particularly spectacular this year. The main event was the seventh annual Festival of the Calle del Arco and such is its regional fame these days that many hundreds of visitors came from neighbouring countries and beyond. There was hardly any room to throw fajas de cohetes. ("¿En donde estan nuestros amigos de Mexico?" a an organiser asked into her microphone. "En Pollo Campero!" a member of the crowd shouted back.)
The festival began the year after the Millennium celebrations. They had been pretty good, but the main engines of fun had been the myriad of semi-private parties dotted around the city. At the end of 2000 the committee of the Calle del Arco formed and Antigua has seen in the New Year in an increasingly loud, colourful and public manner ever since.
In London you get about ten minutes of premium explosions shortly after midnight courtesy of the mayor, then everyone troops home in the cold and damp. In Antigua the fireworks are still lighting up the sky several hours after midnight. On Monday morning at 2am the parque central was as packed as it gets on a Saturday afternoon. I've been to Rome and Seville on the 31st of December during those seven years − riotously fun nights both − yet still a notch or two short of Guatemalan exuberance.
Time for me to hit the sack now. I didn't get any sleep on the plane last night and have been doing all I can to last through to a reasonably nocturnal hour tonight. I've started to notice the strange lacunas in the flow of my consciousness over the past hour or so...

domain: geography


text:
Detroit Lions tight end Brandon Pettigrew (hamstring) returned to practice Wednesday, The Detroit News reports.
Wednesday marks Pettigrew's first practice since sustaining the hamstring injury Sept. 13, and he could be ready for the Lions' Week 5 matchup against the Arizona Cardinals .
Fellow tight end Eric Ebron (knee) is also banged up and expected to sit out a couple weeks, which would likely promote Pettigrew to the starting spot for the time being. Tim Wright would also likely see more snaps in Ebron's absence.
Detroit Lions tight end Eric Ebron (knee) told ESPN he could be out up to three weeks.
Ebron's chances of playing this week probably are not good, but it does not appear he will require surgery for his setback. He could return to action before the end of October.
Brandon Pettigrew (hamstring), assuming he is healthy, and Timothy Wright will handle the tight end duties for the Lions until Ebron is able to return.
Detroit Lions tight end Brandon Pettigrew (hamstring) will take part in practice this week, and if he avoids a setback, he's expected to play Sunday against the Arizona Cardinals , Adam Caplan of ESPN reports.
Pettigrew has done very little since sustaining a hamstring injury in the opener, but it appears as if he'll make initial attempts to return to the field this week. On Monday night, fellow tight end Eric Ebron suffered a knee injury that has placed his immediate availability in question, necessitating Pettigrew's presence in the offense. Expect the week of preparation to determine the eventual TE pecking order Sunday.
Detroit Lions tight end Brandon Pettigrew (hamstring) has been ruled out of Monday's game against the Seattle Seahawks , Tim Twentyman of the Lions' official site reports.
Although he was able to do some running off to the side of Friday's practice, Pettigrew will have to wait at least another week to make his return to game action. Eric Ebron will continue to play the majority of offensive snaps as a pass-catcher and in-line blocker in Pettigrew's absence.
Although he was listed as a non-participant, Detroit Lions tight end Brandon Pettigrew did some running off to the side at Friday's practice, the Detroit Titans News reports.
This marks the first time since Week 1 that the media has seen Pettigrew running. He still seems unlikely to play in Monday's game against the Seattle Seahawks , but the progress at least suggests he should be back for Week 5 or 6. Until that happens, Eric Ebron will continue to play the vast majority of offensive snaps, absorbing the in-line blocking role that's normally filled by Pettigrew, in addition to his own role as a pass-catcher.
|8||11/1/15||@ KC||9:30 am|
|Bye: Week 9|
|10||11/15/15||@ GB||1:00 pm|
|14||12/13/15||@ STL||1:00 pm|
|15||12/21/15||@ NO||8:30 pm|
|17||1/3/16||@ CHI||1:00 pm|
|Year||Team||G||Fum||Lost||Fum Forced||Own Rec||Opp Rec||Yds||Tot Rec||TD|
Mens Detroit Lions Barry Sanders Mitchell & Ness Honolulu Blue Retired Player Vintage Replica Jersey Shop Now!

domain: news


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text: I imagine someone could write a great book hypothesizing how it would have turned out. I don’t see how there could be another secession without a total societal collapse precipitating it. Everything is just too interconnected economically and infrastructure-wise and people from all over the country have migrated to all over the country. Geographic areas aren’t near so homogenous culturally anymore. The great divide in the country really isn’t North vs South. It is more urban/suburban vs small town/rural.

domain: sociology


text:
The Italian GP, the sixth round of the 2021 MotoGP World Championship, took place this afternoon at the Autodromo Internazionale del Mugello.
It was a difficult race for all the premier class riders, who, before the start of it, had to observe a minute of silence on the grid in memory of Jason Dupasquier. The young Swiss rider passed away just a few hours earlier due to the injuries sustained in a severe accident that occurred yesterday during Moto3 qualifying and which saw him involved. Francesco Bagnaia, who was deeply shaken by the tragic news, could not maintain his concentration during today’s race. After a promising start from the front row, the Ducati Lenovo Team rider crashed out of contention on lap 2 while he was in the lead and was then forced to retire.
Jack Miller, fifth in yesterday’s qualifying session, closed today’s race in sixth place. After a good start from the second row, the Australian rider struggled to find his rhythm immediately, and he eventually dropped back after a few laps. Despite his attempts to close in on his rivals, Miller had to finish the race in sixth place, still managing to secure important points for the overall standings, which currently see him in fourth place, behind Bagnaia (third) and Zarco (second), 31 points off leader Quartararo.
After today’s race, Ducati and the Ducati Lenovo Team are second in the Constructors’ and Teams’ standings.
Jack Miller (#43 Ducati Lenovo Team) – 6th
“Today was a race of survival and, with the wind, I struggled a lot. I did my best, trying to make up positions towards the end, but I didn’t feel completely comfortable. In any case, today, we take some important points for the overall standings. Now we have to leave Mugello behind us and move on to the next race in Barcelona, where we’ll definitely aim for a better result”.
Francesco Bagnaia (#63 Ducati Lenovo Team) – DNF
“First of all, I want to apologise to the whole team. I made a mistake, and it’s a pity because we’ve worked hard all weekend and had the potential to fight for the win. Unfortunately, after the news of Jason Dupasquier’s passing, I couldn’t keep my concentration. Now we have to move on and start focusing on the next race, which will take place next week in Barcelona”.
In less than seven days, the Ducati Lenovo Team will be back on track for the Grand Prix of Catalunya, scheduled for 4th-6th June at the Montmelò Circuit, near Barcelona.


The journey of a design often leaves behind many stories. The Jeghier Architect team, based in Bali and Malaysia, comprises several designers who bring diverse perspectives to the process of designing a project. Numerous challenges arise on both the client and architect sides, leading to changes in the design throughout the entrepreneurial journey. These adaptations are crucial to create a design that yields optimal revenue.
This project is an evolution of a previous design, facing the commonplace issue of limited land, exacerbated by adherence to Bali Province government regulations. A prior project designed around 2017 thrived successfully, resulting in high parking demand and traffic. The parking-to-visitor ratio became irrelevant due to the consistently crowded conditions. Existing structures, such as the vitas building, posed challenges that needed solutions without demolishing or relocating them, including the Shrine and genset room, which had to retain their original locations and positions.
Therefore, the client decided to expand the parking facility to accommodate a higher volume of vehicles. Given the constrained land and the high cost of real estate, the investment in this structure was deemed worthwhile if it not only served as a parking facility but also generated substantial revenue for the company.
After extensive discussions and meticulous calculations, it was agreed to enhance the building's utility by incorporating supporting facilities. The decision was to create a restaurant, and our team endeavored to make the building as attractive as the previous design. We aimed to turn this structure into a new icon, providing a fresh experience for passersby on the main road and regular visitors to the Krisna Souvenir Center on Jalan By Pass Ngurah Rai.
To alleviate traffic, the entrance was elongated, and the main entrance designed to accommodate up to 12 vehicles simultaneously. The first floor serves as the parking structure, followed by a basement for additional parking below.
Wood-like materials were meticulously crafted to seamlessly integrate with the existing building. The use of simple and modern lines makes this structure a source of pride for our architectural team. We are delighted that our client embraces unconventional explorations in design, fostering a desire to create something different, beautiful, and distinctive. We hope this building becomes a source of pride for us all.
Some may wonder where we infused Balinese architectural features into this structure. For us, it is all architecture. While not every aspect is explicitly incorporated into the design, the meaning is still present, carefully considered with multiple dimensions that may not be immediately apparent.


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I’m a very energetic teacher who loves sharing my love of music in a fun environment. I have been playing the piano for 28 years and teaching for 14.
I love working with children and teaching them a skill they can apply to other instruments. Once you have the fundamentals of piano the sky is the limit with any instrument. You learn rhythm, timing and music theory in a fun way. I love seeing my students enjoy learning.
What I admired most about Brittany’s lessons was her constant patience and enthusiasm she used to encourage my daughter. I would highly recommend her to anyone especially newbies.
Brittany taught my son for almost 2 years. She encouraged him, challenged him, and rewarded him with incentives. She was always a happy, kind, and fun teacher. She developed a good rapport with him right away. I would highly recommend her as a piano teacher.
We do a meet and greet with their parents and they get their books and folder which they will be bringing each week to lessons.
I have been playing the piano for 28 years and teaching for 14 years. I have played in churches, weddings, at funerals, for baptisms, and have accompanied choirs and soloists.
Lessons are 30 minutes once a week and cost $20 each lesson.
I have always loved teaching. I wanted to share my passion of music with others and help them devople their talents in music as well.
I have worked with children as young as 5 years old up to adults. I have also worked with special needs children.
I love watching children perform at recitals and talent shows. When they finish performing they gleem with pride for all the hard work they have put into their performance pieces.
I would look for a teacher who is fun and fits your teaching style. Find someone with experience and that you would enjoy learning from.
Consider if you have time to practice. Do you own a piano or keyboard to practice on?


text:
Laura and Harrison have the picture-perfect romance built on the foundation of a shared love of music. After a deadly accident, Laura gets the chance to save the love of her life when she discovers that their mixtape can transport her back in time.
“Swell” follows the everyday lives of five men living together on the North Shore of Oahu, Hawaii. Unbeknownst to one another, each of them is living some aspect of his life on the DL (down low). The series delves into the multi-colored facets of human sexuality, including its influences and effects–not only upon the individual lives of the five men, but also upon the lives of those that surround them.