Married at First Sight Australia: Ines and Sam sleep together
Married At First Sight Australia is a popular reality series which sees singletons tie the knot before they have had a chance to meet. Season six originally aired in 2019 and it has finally made its way to the UK, so fans have been catching up. Express.co.uk has all you need to know about how many episodes are in MAFSA season six.
How many episodes are in MAFSA season 6?
Married At First Sight Australia season six first arrived in the UK at the start of 2021 and 12 couples featured as part of the season.
The sixth instalment originally aired in January 2019 and it came to an end in April of that year.
Fans in the UK have seen the couples meet for the first time, exchange vows and go on their honeymoons.
They must also move in together and meet each other’s families before making a final decision on whether they wish to remain together.
There was plenty of drama and scandal from the outset, including affairs and harsh exchanges.
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The episodes have been airing on E4 from Monday to Friday each week, and they are available on ALL 4.
Season six is made up of 41 episodes, so there is still plenty left to come for fans to enjoy.
As of the time of writing, a total of 17 episodes have aired with episode 18 due to air tonight (January 27).
Tonight’s episode will provide an exciting twist as two new couples will be added to the experiment.
Fans will be keen to find out who these couples are and whether they have the potential to stay together.
For viewers in the UK, the series is expected to continue airing on weekdays up until mid-February.
The season reunion finale is split into two parts, which will air on consecutive days.
In the finale episodes, the couples will return to speak to the panel of experts one last time.
They will get to look back at some never-before-seen footage and discuss their actions and decisions throughout the experiment.
This acts as the perfect opportunity for individuals to confront their partners, so expect plenty of drama.
Ines and Sam Married At First Sight Australia: What happened? [INSIGHT]
Married At First Sight Australia season 6: Which couples are together? [EXPLAINER]
Mike and Heidi Married At First Sight Australia: Are they together? [INSIGHT]
Fans have already seen a couple of fights during the dinner parties, which act as an opportunity for individuals to raise any issues.
For those who have already been looking for MAFSA spoilers, they would have seen only two couples are still in relationships with each other.
Jules Robinson and Cameron Merchant, who were the first couple to marry, decided to tie the knot in real life and they now have a son together.
Martha Kalifatidis is also still in a relationship with her ‘husband’ Michael Brunelli and they are very supportive of each other.
The premise of the series has become so popular that it has inspired spin-offs in the UK, American and elsewhere.
The idea came from a Danish version of the series, in which the couples are legally married before they have a chance to meet in person.
Viewers have taken to Twitter to discuss season six of the Australian version, with one saying: “I can’t deal with series that I’m up to date on.
“I can’t even wait a day to see the next ep of Married At First Sight Australia, it’s so addictive I am living for the drama.”
Another fan said: “My whole life has basically been taken over by Married At First Sight Australia now.”
A third viewer said: “Married At First Sight Australia is the most addictive show ever and I have watched every reality tv show there is.”
Married At First Sight Australia airs on E4
Source: Read Full Article


24-25 February 2015. Klaus Iohannis pays an official visit to Chisinau
15:26 | 08.02.2021
Category: REPUBLIC OF MOLDOVA: THREE DECADES OF INDEPENDENCE.
Retrospective of events
Romania’s President Klaus Iohannis paid a visit to Moldova on 24 February 2015, at an invitation by his Moldovan counterpart Nicolae Timofti.
This visit was practically announced back on the period of the elections, when the staff of Iohannis at a news conference informed that he would pay his first visit abroad to Moldova.
The presidential polls in Romania from November 2014 were dynamic and with spectacular changes of situations. After the first round of the ballot, candidate Victor Ponta had a comfortable advance of 10 per cent and it seemed that nothing could change the situation. Yet, in the runoff, an unprecedented mobilization of the pro-Iohannis electorate took place and he gained a clear-cut victory with the score of 56.56 per cent to 45.56 per cent.
Nevertheless, the visit to Chisinau could not be organized immediately after the swearing in office of the new Romanian head of state. Parliamentary elections were held in Moldova in late 2014 and the new Gaburici government was sworn in office as late as on 18 February 2015.
The visit took place on 24 and 25 February and had a comprehensive and quite well calculated agenda.
In Moldova’s capital, Klaus Iohannis had a long discussion with his counterpart Nicolae Timofti. The national media at that time said that the discussions had had a cordial character and were focused on Romania’s role in the process of Moldova’s European integration. Klaus Iohannis firmly promised that, in the discussions with western chancelleries, he would tackle issues on Moldova’s European integration and would promote the need of a substantial support for the reforms in the country. The sides also brought for discussion the bilateral economic relations in full growth, as well as other aspects of the Moldovan-Romanian relations.
During the visit, Klaus Iohannis also had meetings with Prime Minister Chiril Gaburici and Parliament Speaker Andrian Candu. Discussions with the leaders of the parliamentary parties took place afterwards.
The Chisinau mass media could learn more about the visions of the Romanian head of state from his speech given in front of students of the Moldovan State University. Next are just some of the fragments of this programmatic speech and full of pragmatism, which fully characterizes Iohannis:
"I want to refer to two fundamental values which govern the relation between us: solidarity and identity. And I sum up these two notions, interdependent in our case, through the European project assumed by Moldova in relation with Romania. The cooperation between us proves how important is to have an advocate on this way. Moldova has a great chance which we did not have at the moment of accession. This chance is called Romania, a full-fledged member of the European Union, presently the most redoubtable supporter of Moldova in the integration process,’’ the Romanian president said.
In continuation, Klaus Iohannis touched upon the things which unite the two countries and define them as part of the European civilization. At the same time, he positively appreciated the efforts of Moldovan governments, which had made concrete steps in the integration process.
"In the last years, you have made important efforts for rapprochement with the European Union. Moldova managed to fully fructify the status of member of the Eastern Partnership, getting the cancellation of visas for the Moldovan citizens and the signing, along with Ukraine and Georgia, of the Association Agreement with the European Union, including the component of changes’ liberalization. These are resolute steps for the integration, but they are not enough. Therefore, the efforts must continue,’’ Iohannis stressed in his speech.
The president of Romania also referred to the complicated regional context, taking into account the Russian-Ukrainian relations, from the viewpoint of Crimea’s annexation and the Donbas war and the need to strengthen Moldova’s statehood. The Romanian head of state said that ‘’we experience troubled times, even at our borders, a prospect which complicates even more Moldova’s endeavours…. In the present regional context, marked by instability, Romania’s duty is to contribute to the guaranteeing of Moldova’s European prospect. For us, the support provided to Moldova does not boil down only to the institutional one. It is about a deepening of the cooperation in all sectors, of the economic relations, cultural and political dialogue not only at the state level, but also between local communities. Also, we should back the projects which lead to the enhancement of Moldova’s energy independence, so that this state becomes independent up to the end,’’ Klaus Iohannis noted.
Iohannis reminded the difficult and contradictory process through which Romania had gone in its European integration process, noting that only the consolidation of all political forces and of the civil society had allowed a good carrying out of the events. ‘’One cannot edify anything sustainable without political stability, without consensus, especially without a major project assumed by the state’s decision-makers. The institutions work and produce concrete and constant results during time, only if there is peace, perseverance and patience, if there is a predictable way and enough will to succeed. I am sure that the history of this country will be written with much hard work in the years to come; yet, the fate of the state is given namely by the courage of the citizens to assume important goals,’’ the Romanian head of state said, in the applauses of those present at the meeting at the Moldovan State University.
In the end, Klaus Iohannis expressed confidence that Moldova’s efforts would not be in vain. The consistent continuation of the reforms, cooperation between the pro-European political forces and involvement of the civil society are compulsory conditions for the achievement of the courageous objective we have assumed.
Unfortunately, not at all simple times followed in Moldova. The fragmentation of the pro-European political spectrum, the robbing of the banking system, the crisis of justice and the complicated international context essentially diminished the pace of reforms and our reliability in EU. Against this background, the visit by Klaus Iohannis to Moldova in February 2015 and his speech at the Moldovan State University proves that the Romanian head of state tackled the subjects discussed in Chisinau with much far-sightedness and good-will. Unfortunately, the evolution of the events in Moldova was not marked by cohesion and coherence and the goals set at that time remain unachieved. Anyway, the visit to Moldova by Klaus Iohannis fit into a string of international contacts of great prestige for Moldova and represented a live evidence of the neighbour country to help us on our road towards a free and prosperous state.
Chisinau, 4 February /MOLDPRES/.


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4. Micro cheating
If you have found out or seen your partner flirting with another person as if it doesn’t mean anything, then you should start questioning things.
There are certain types of people who believe that flirting is not important because nothing serious happens from it, but for others, it can be disturbing because it gives the impression that the person is available.
If your partner doesn’t present themselves as being in a relationship and gives the impression that they are single, it means they are looking for someone else and do not want to openly display their relationship with you.
It is true that some people are very charismatic, and often their intentions can be misunderstood, but when approached, they make their intentions clear.
If you want to find out more about cheating, then you should read the book Cheating in a Nutshell: What Infidelity Does to The Victim

domain: sociology


The Maserati Levante S is the closest you’ll get to a Ferrari SUV… for now
Next year, Ferrari will launch the most controversial car in its 81-year history. The Purosangue (‘Thoroughbred’ in English) is a super-SUV to rival the Aston Martin DBX and Lamborghini Urus. Enzo wouldn’t have approved, but the shareholders surely will.
Can’t wait that long? The 430hp Maserati Levante S is the closest you’ll get to an SUV powered by prancing horses. Its ‘F160’ V6 engine is based on the F154 V8 from the Ferrari 488. Does it retain some Maranello magic?
This is the second rung on the Levante ladder, which clambers up from the 350hp base model to the 580hp V8-engined Trofeo. At £83,435, it squares up to the Range Rover Sport and Porsche Cayenne, plus more exalted versions of the BMW X5, Mercedes-Benz GLE and Jaguar F-Pace. However, with just 11 Maserati dealers across the UK, it will be a rarer sight than any of them.
Exclusivity is a big part of the Levante’s appeal, as is how it looks. That clenched-teeth grille makes a bold statement without being too gauche (take note, BMW), while its profile is coupe-like without seeming contrived (yep, BMW again). Only the rather generic rear-end styling lets it down.
Top tip: go for GranSport rather than GranLusso spec (seen here) to best accentuate the sculpted sides and muscular haunches.
Forgetting Ferrari for a moment, the trident badge has an allure all its own – recently bolstered by the new MC20 supercar.
Maseratis of the past have been unorthodox and flawed, but – with the notable exception of the Ghibli diesel – they have never been dull. Nor is the Levante S.
How could it be with this engine? The 3.0-litre twin-turbo V6 is glorious: zingy and athletic in the mid-range, then exuberant and visceral as the revs rise.
Driving all four wheels via an eight-speed automatic gearbox, it propels the portly 2,109kg Levante to 62mph in 5.2 seconds – on par with a Porsche Cayenne S. Its vocal repertoire ranges from subtle snarl to piercing howl, punctuated by crackles from the four tailpipes.
All Levantes ride on air suspension, which strikes a decent balance between comfort and cornering composure. You can also raise the car’s height for off-roading or hunker it down for high speeds.
Overall, the Maserati is softer and less tenacious than a Cayenne, but still fluid and enjoyable to drive. Its steering is well weighted and the brakes feel powerful.
Only its sheer size, and the prospect of fuel economy in the high teens (the official figure is 22.1mpg) may temper your enthusiasm.
Being an SUV, of course, you can bring the family along, although the Levante is best suited to four (the middle rear passenger must perch on a hump) and its tapered tailgate cuts sharply into boot space. Another top tip: leave the labrador at home.
Frankly, the Levante is too plush for dog duties anyway. Rich leather swathes the doors and dashboard, the three-spoke steering wheel looks great and the metal gearshift paddles are deliciously tactile.
Sadly, the abundance of hard plastic switchgear doesn’t befit a luxury car, while the touchscreen infotainment system is clunky. At least Apple CarPlay and Android Auto are standard, so you can simply plug in your phone.
Like many charismatic Italian cars, then, the Levante is far from perfect. It’s thirsty, not particularly practical and perceived quality could be better. Yet its glamorous badge and glorious engine offer an irrational, emotional sucker-punch that no German rival – nor indeed the Range Rover Sport – can match.
It takes a lot to make an SUV feel exotic, but a Ferrari engine does just that. Maybe we shouldn’t be so worried about the Purosangue after all.
TOP SPEED: 164mph
CO2 G/KM: 263
MPG COMBINED: 22.1
Tim Pitt writes for Motoring Research


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r/Citra: For all things related to the Nintendo 3DS emulator Citra. ... Super Mario 3D Land running slow on a good system. Question. ... Settings. Fullscreen. 29. 8 ...
Super Mario Land. Super Mario Land was released on April 21, 1989 in Japan, August 1, 1989 in North America and September 28, 1990 in Europe. The game was Mario's first appearance on the Game Boy. It was a launch title for the Game Boy for both Japan and North America.
Jogo rodando liso no citra Canary - 9d89868, irei estar postando novos testes em breve; Veja EMU NEWS 03 e confira ass novidades dessa versãohttps://www.yout...
Super Mario Odyssey Summary. Super Mario Odyssey requires at least 4 GB of RAM to boot. For playable performance, a high single thread performance CPU is required since the game mainly works off of a single core. This goes in favor of Intel CPUs for emulation. It has some graphical issues, but the game play isn’t affected for the most part.
Paper Mario 3D Land is a fabulous fan made Mario game that blends the action platforming gameplay of the Super Mario 3D Land games with the charming 2.5D visuals of the Paper Mario series and lots of great new features and power-ups! Paper Mario 3D Land is easily one of the most highly polished fan games we’ve come across. The visuals, audio ...
Super Mario 3D Land is a 3D Mario platformer that came out in November 2011 for the Nintendo 3DS (retail version; as of 2012 the game is available from the … Video Game / Super Mario 3D Land. Go To.
Super Mario Land 2: 6 Golden Coins October 21, 1992 GB; 3DSE; Mario's second platforming adventure on Game Boy introduces the evil Wario, who has taken over Mario's castle in his absence. Mario must gather the six golden coins scattered throughout Mario Land to reopen the castle gates and defeat his twisted counterpart.
Download Super Mario 3D Land Guide (Walkthrough) for iOS to this is a complete walkthrough guide of the video game Super Mario 3D Land Guide (Nintendo 3DS). Use it to find your way through the ...
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The Super Mario Galaxy (3D All Stars) is a Nintendo Switch exclusive. It will not be released in any other platform besides the Nintendo Switch. Super Mario Galaxy (3D All Stars) Gameplay. An Adventure Through The Universe. The stars and the vast outer space awaits in this out of this world...In Super Mario World Mario and Luigi must once again save their favorite princess as Bowser and his minions try to take their revenge on the plumber brothers in Dinosaur Land. The Super Mario series took the Super Nintendo by storm with this instant classic.
Super Mario 3D All-Stars is a 2020 compilation of 3D platform games for the Nintendo Switch. It commemorates the 35th anniversary of Nintendo's Super Mario franchise, with high-definition ports of Super Mario 64 (1996), Super Mario Sunshine (2002), and Super Mario Galaxy (2007). The compilation was released on September 18, 2020.
Super Mario 3D World is now playable through multi player with four characters in 3 different lanes. Each Character has its unique abilities and can help each other throughout the game. New maps, enemies and play style is being implemented and the new ability where you can transform in to a cat that gives you the ability to be in all 3 lanes ... Super Mario 3D Land Review (3DS) Robert Workman - May 4, 2012 Mario returns with a fresh, familiar new adventure – and one that vindicates the Nintendo 3DS to boot.
The Super Mario Galaxy (3D All Stars) is a Nintendo Switch exclusive. It will not be released in any other platform besides the Nintendo Switch. Super Mario Galaxy (3D All Stars) Gameplay. An Adventure Through The Universe. The stars and the vast outer space awaits in this out of this world...
Mario game by Nintendo. Tree loses Super Leef. Chick gets kidnapped by giant turtle. Plumber stomps on things to rescue said chick. "The green one" may or may not be relevant. Classic Mario gameplay (go through stages, stomp on baddies, hit the flagpole) on a 3D plane. Super Leaf gives you Tanuki suit (or Kitsune as Luigi). Nov 14, 2011 · Super Mario 3D Land wastes a bit of time up front with some levels that are a little too basic, but once it gets going, it reveals some terrific moments that make the 3DS make sense. Swing by Toad's place to grab some power-ups and listen to that "hey, you're at Toad's place" music that originally appeared in SMB3.
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Mods, discussions and more by the Super Mario 3D Land Modding Community
3DS0225 - Super Mario 3D Land (Kor) 3DS0225 - Super Mario 3D Land (Kor) Description: Image: Size: 289.72MB: Download: Register to Download this file: Rating: 2/5 : 5 ... Mar 19, 2014 · Super Smash Bros. 3DS director Masahiro Sakurai has revealed a brand new stage in the handheld brawler. It’s based on Super Mario 3D Land and continually scrolls to the right.
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8.8:. Gamespot slapped it with a meager 8.0, while other outlets such as IGN, Game Informer and GamePro gave it higher scores. While other reviews had their reasons for lowering the score in the game being unoriginal or being overly easy for much of the game, Gamespot's written justification was just "[Super Mario 3D Land has] limited StreetPass and gyrometer features."
Jul 09, 2017 · User owenkee uploaded this Super - Super Mario 3D Land Super Mario 3D World Super Mario 64 Super Mario Galaxy 2 PNG image on July 9, 2017, 1:21 am. The resolution of this file is 2876x3220px and its file size is: 604.16 KB. Pokemon Setting Sun. Pokemon Umbra Moon. Pokemon Omega Steven (Region Free) [Decrypted] 3DS Rom for Citra. Pokemon Super Mystery Dungeon. Pokemon Alpha Sapphire. Pokemon Omega Ruby.
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Podemos decir que en Super Mario 3D World encontramos la mezcla casi perfecta entre los juegos más recientes de Mario, New Super Mario Bros., Super La dificultad del juego es superior a la de Super Mario 3D Land pero inferior a la de Super Mario Galaxy (especialmente con las comodidades...
Nov 19, 2013 · Nintendo fans listen up; you can get a free copy of Super Mario 3D Land if you register a 3DS or 3DS XL on Club Nintendo . The promotion is running from 27 th November to 13 th January. During ... Sep 18, 2020 · Super Mario 3D All-Stars is out now, and, with that, we’re here to help you collect every Power Star in Super Mario 64‘s Shifting Sand Land. Shifting Sand Land Star 1: In the Talons of the Big Bird. Head over to and run up one of the chequered pillars and jump kick Klepto the condor to release the Power Star from his talons.
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domain: hobby


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‘First Pluck the Eyes Out’: mutilation in the poetry of Wopko Jensma
Although the act of mutilation is a widespread cultural and aesthetic practice, its pathological manifestations prompt shock, dismay and serious concern. Wopko Jensma has appropriated these qualities of aberrant mutilation to foreground social despair in bizarre cameos of racial oppression and underclass misery. Jensma’s use of mutilation as a poetic resource is typical of his experimental style and departure from convention. His poetry of fragmentation, bizarre pathological motifs, selfmutilation, sado-machoism, and conflicted speakers dispersed across multiple subjectivities undermine the reductive politics of racial essence and privilege the marginalized in society. Image, narrative and diction coalesce in dissonant aesthetic strategies to express the anguish and psychological annihilation emanating from human degradation and despair.
Keywords: Mutilation, social despair, oppression, experimental poetry, dissonant aesthetics

domain: artistic


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|Swap Coordinator:||museofthemorning (contact)|
|Swap categories:||Art Artist Trading Card (ATC) Mail Art|
|Number of people in swap:||20|
|Location:||Regional - United States|
|Type:||Type 3: Package or craft|
|Last day to signup/drop:||December 1, 2021|
|Date items must be sent by:||December 13, 2021|
|Number of swap partners:||1|
It looked like the ATC swaps were getting a little low- so I thought I would add a couple!
You will send ONE partner an ATC made in any art style or medium EXCEPT - NO STICKERS!
An ATC is 2.5x3.5 inches in size and should be on heavy cardstock or watercolor paper.
I am putting the rating at 4.5, but I will be going through each profile and you should have no recent 1s or 3s. I will angel if necessary, but let's not make that have to happen!!
The post office might be a little delayed right now, so give a little extra time for envelopes to arrive.
You should rate a 5 if you receive an ATC that is not sticker slapped. Hearts are up to you.
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Cesar Gracie: St. Pierre should be stripped of his UFC Welterweight titlePosted on December 7, 2011, 05:19 PM by Trent Reinsmith
Carlos Condit, who had been scheduled to face Josh Koscheck on the same card has been moved into St. Pierre’s spot and the fight will be for the interim Welterweight title, something that Diaz’s trainer/manager, Cesar Gracie is not fully in agreement with. In fact, if Gracie had his way, St. Pierre would be stripped of the title and Diaz and Condit would be fighting for that title.
“My idea on that, and I haven’t spoke to Dana about it yet, is that they’re making this an interim fight and I think it should be that championship fight because since the Jake Shields fight, by the time GSP fights again, it’s going to be about two years. A champion being out for a couple years? My idea is that you should actually strip him (of the title.) Being a fighter is being healthy, that’s just part of it. You can’t just take a couple years off and I know these things happen and I’m sure it’s not his fault. It’s kind of like Nick deserves a championship fight and with an interim title and there’s always the people who say, ‘you’re not the real champion.’” – for more see MiddleEasy.com
What do you think LowKick’ers, does Gracie have a point?
Photo: Georges St. Pierre at the UFC 100 press conference at the Mandalay Bay Hotel and Casino on July 9, 2009 in Las Vegas, Nevada. Francis Specker

domain: news


CHATHAM – When the Chatham Borough Council meets Monday, Dec. 10, it’s expected the governing body will entertain a resolution submitted by Councilman Robert Weber calling on the state Legislature to reject any bill that would legalize the sale and recreational use of marijuana.
But the council is holding back on introducing an ordinance, also strongly advocated by Weber, that would ban the cultivation of marijuana, retail shops for sales of marijuana for recreational use, and even retail sales of marijuana for medical use in Chatham.
Prompted by the prospect that the state Legislature was on the verge of a vote to legalize marijuana with certain restrictions, Weber made a detailed presentation urging the council to act on both a resolution and an ordinance at the governing body’s meeting on Nov. 26 — but was met with a cautious reception.
Weber began by alluding to an online survey the borough created in October to sample “how our community might feel” about state legalization of marijuana use for recreation, and whether Chatham should ban retail sales of marijuana for recreational use, facilities that grow marijuana, and retail sales of marijuana for medical use.
Only 218 residents took the survey. Those respondents decisively opposed state legalization of marijuana (119 to 96) and favored bans in Chatham on facilities to grow marijuana (146 to 69), cultivation and retail sales of marijuana for recreational use (142 to 74) — but just as decisively opposed a ban of sales of marijuana for medical use, 131 against a ban to 83 in favor.
Said Weber, “I would like to see the council send a resolution to the state Legislature opposing the legalization of marijuana. And I’d like to see this council adopt an ordinance banning a marijuana shop in Chatham.”
The councilman explained, “The state is fast-tracking bills to legalize the recreational use of marijuana, and expand the scope of medical marijuana.” Weber said there was nothing in such legislation that could stop “unscrupulous doctors” from writing marijuana prescriptions when alternatives were available.
As for determining whether a driver is impaired by marijuana, Weber pointed out that, unlike alcohol, there is no test for marijuana intoxication, and under state proposals, “The folks who are going to be responsible for regulating these laws are local law enforcement.”
If the state legalizes marijuana, Weber noted, “The law could allow towns to ‘opt out’ within six months” of the law taking effect if the towns “prohibit, by ordinance, cultivation and marijuana retail sales.”
He said that in Morris County, the municipalities of Chatham Township, Florham Park and Parsippany “have already opted out” by adopting such ordinances in advance of potential state legalization.
Weber argued that if the state legalizes marijuana, “Businesses can’t wait to open marijuana retail shops in New Jersey. It’s a cash cow.” Inevitably, Weber said, greater availability of marijuana meant the drug would find its way into the hands of young people, and there were no studies defining the long-term health effects.
He also said that while only 218 residents took the borough’s online survey, all are “registered voters.” Weber concluded the council should amend the borough’s land use code by ordinance to declare the cultivation, manufacturing and retail sale of marijuana a “prohibited use.”
Council members, however, had questions.
Mayor Bruce Harris observed, “We know alcohol has bad health effects. We know tobacco has bad health effects. How do you distinguish? There’s no evidence marijuana is addictive.”
Councilwoman Victoria Fife asked just what a proposed “expansion” of medical marijuana meant, and was told it would expand the number of medical marijuana dispensaries in the state; currently there are only six.
‘Helps Her Pain’
Councilman Thaddeus Kobylarz told Weber, “I have a good friend with M.S. (multiple sclerosis) and marijuana is the only thing that helps her pain — and she’s a Chatham Borough resident.”
Kobylarz said he and Councilman Len Resto had consulted the nonprofit Madison Chatham Coalition, which is dedicated to reducing substance use in the community, especially among young people, and the Coalition “is willing to sponsor a series of discussions, featuring people pro and con — experts.” The Coalition itself could not take a position on legislation, he said, because it receives government funding. Provided the Coalition could line up authoritative speakers and arrange balanced presentations, Kobylarz said, “The topics would be medical marijuana, effects on teenagers, and social policy.”
Kobylarz told the council, “I want ‘town halls’ to hear from experts. Give it a chance.”
Mayor Harris sided with the less urgent approach, given the borough would have six months to “opt out” if the state legalizes marijuana. “Bob (Weber) can write a resolution (to the Legislature) and we’ll vote on it on December 10,” the mayor said. “I see no consensus for an ordinance.”


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Preservation of original organic components in fossils across geological time is controversial, but the potential such molecules have for elucidating evolutionary processes and phylogenetic relationships is invaluable. Chitin is one such molecule. Ancient chitin has been recovered from both terrestrial and marine arthropods, but prior to this study had not been recovered from fossil marine mollusks.
Organics consistent with β-chitin are recovered in cuttlebones of Mississaepia mississippiensis from the Late Eocene (34.36 million years ago) marine clays of Hinds County, Mississippi, USA. These organics were determined and characterized through comparisons with extant taxa using Scanning Electron Microscopy/Energy Dispersive Spectrometry (SEM/EDS), Field Emission Scanning Electron Microscopy (Hyperprobe), Fourier Transmission Infrared Spectroscopy (FTIR) and Immunohistochemistry (IHC).
Our study presents the first evidence for organics consistent with chitin from an ancient marine mollusk and discusses how these organics have been degraded over time. As mechanisms for their preservation, we propose that the inorganic/organic lamination of the cuttlebone, combined with a suboxic depositional environment with available free Fe2+ ions, inhibited microbial or enzymatic degradation.
Citation: Weaver PG, Doguzhaeva LA, Lawver DR, Tacker RC, Ciampaglio CN, Crate JM, et al. (2011) Characterization of Organics Consistent with β-Chitin Preserved in the Late Eocene Cuttlefish Mississaepia mississippiensis. PLoS ONE 6(11): e28195. https://doi.org/10.1371/journal.pone.0028195
Editor: Anna Stepanova, Paleontological Institute of Russian Academy of Science, United States of America
Received: May 9, 2011; Accepted: November 2, 2011; Published: November 23, 2011
Copyright: © 2011 Weaver et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: FTIR analysis in this study was supported by NSF grant EAR-0929898 to RT. LD received travel and subsisitence support from the Swedish Academy of Sciences and PW received travel support from the NC Museum of Natural Sciences and the NC Fossil Club. FAI Materials Testing Lab Inc. supported the study through the employment of JC, who contributed to all aspects of the study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have the following competing interest: JC is an employee of FAI Materials Testing Laboratory. There are no patents, products in development or marketed products to declare. This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials.
Chitin is a complex amino-polysaccharide produced by many living organisms, including arthropods, mollusks, nematodes, algae and fungi. It is, after cellulose, the second most abundant biopolymer found in nature . Chitin is a linear polymer consisting mainly of β-(1→4)-linked 2-acetamido-2-deoxy-β-d-glucopyranose units and partially of β-(1→4)-linked 2-amino-2-deoxy-β-d-glucopyranose . In nature, chitin occurs in three polymorphs, either α or β-allomorphs or in γ-form , distinguished by differences in the types of chemical linkage employed or by the orientation of the constituent micro-fibrils relative to these linkages .
Based primarily on morphological comparisons with extant forms and not on chemical analyses of fossils, chitin is hypothesized to have been a major structural component of invertebrates since the Cambrian, but it may have originated sometime in the Proterozoic . Detection of chitin in fossils is not frequent. There are reports of fossil chitin in pogonophora, and in insect wings from amber . Chitin has also been reported from beetles preserved in an Oligocene lacustrine deposit of Enspel, Germany  and chitin-protein signatures have been found in cuticles of Pennsylvanian scorpions and Silurian eurypterids .
Initial examination of cuttlebones of Mississaepia mississippiensis  from the Late Eocene (34.36mya)  from the Yazoo Clay, Jackson Group, Hinds County, Mississippi (Figure 1) by light microscopy revealed yellowish-brown sheet-like structures that appeared to have been originally organic. EDS examination of these structures revealed phosphorus. These observations led to questions 1) could any original organics be preserved in these cuttlebones? 2) What mechanisms might have preserved original endogenous organics? The present paper reports evidence of organics consistent with β-chitin from the Late Eocene cephalopod M. mississippiensis and presents evidence of ultrastructural and environmental factors which might have contributed to their preservation.
Materials and Methods
Nine cuttlebones of M. mississippiensis from collections at The Mississippi Geological Survey were examined. For comparison, five cuttlebones of extant cuttlefish (Sepia sp. NCSM 11399–11403; collections at the North Carolina Museum of Natural Sciences) were examined. Eight fossil specimens (MGS 1943, 1944, 1946–1949, 1956, 1963) and one extant specimen were analyzed using SEM/EDS (Text S1). Specimen MGS 1951 (Figure 2A,B) and extant Sepia specimen NCSM 11399 were cut laterally and longitudinally for ground sections. The remaining portion of fossil specimen MGS 1951 and extant specimens NCSM 11402 and 11403 were de-mineralized for Hyperprobe, FTIR and IHC analyses (Text S1). For comparison, three specimens of extant Loligo sp. pens were de-mineralized for FTIR analysis. Complete methods are given in the supporting data (Text S1).
Scanning Electron Microscopy/Energy Dispersive Spectrometry (SEM/EDS)
Analysis of M. mississippiensis under SEM (Text S1) revealed ultrastructures (Figure 3Aa), between carbonate spherulites, morphologically similar to the chitin membranes found in extant Sepia (Figure 3Cb). Elemental analyses show these “sheet-like” structures in M. mississippiensis contain high amounts of phosphorous (Figure 3B). These sheet-like structures may have been phosphatized during fossilization as is typical of chitin structures in cephalopods, such as gladii and mandibles , . Alternatively these originally organic structures could have served as a template for phosphatization  and some of the original organic material may have been preserved.
To test for presence of original organics, cuttlebones of M. mississipiensis and Sepia sp. were demineralized in HCL (Text S1) then mapped for nitrogen. Nitrogen, a reliable indicator of organic material , was detected in de-mineralized powdered samples of Mississaepia mississippiensis and extant Sepia sp. using a JEOL X-ray Analyzer JXA-85-30F Hyperprobe (Text S1). Though not a specific indicator of chitin, nitrogen signals obtained from powders indicate original organics have been preserved in the fossil. Nitrogen was detected only on places where there was sample and thus is not a byproduct of nitrogen within the epoxy of the carbon tape (Figure 4A,B). Because these samples are three dimensional no quantitative analyses are possible. Overall morphology of de-mineralized material from M. mississippiensis and extant Sepia sp. was different (Figure 4A,B).
Fourier Transform Infrared (FTIR)
Cuttlebones of M. mississippiensis and Sepia sp. were analyzed using FTIR (Text S1) to determine the mineral phase of the carbonates. The cuttlebone of M. mississippiensis shows two peaks of the carbonate ν4 vibration at 700 and 713 cm−1 (Figure 5) which are diagnostic of aragonite . The peaks at 1083 and 854 cm−1 are also characteristic of aragonite . Similar peaks were detected in cuttlebone of extant Sepia (Figure 5). In contrast calcite would show a peak at 876 cm−1 and no peak at 1083 cm−1 . Preservation of aragonite, a meta-stable form of calcium carbonate, rather than the more stable calcite form, indicate the cuttlebones have undergone minimal diagenesis and thus had a greater potential to preserve original organics.
Spectra of Sepia sp. (top) and M. mississippiensis (bottom). Spectra indicate aragonite. By comparison, calcite has peaks at 848 and 876 cm−1and a singlet at 713 cm−1 .
FTIR (Text S1) was also used to examine in detail the organic components preserved in M. mississippiensis. After demineralization of extant Loligo sp. pens (squid pens are commonly used as the standard for β-chitin), Sepia sp. and fossil M. mississippiensis, spectra (Figure 6A) were obtained and band assignments (Table 1) were made according to published works , . Spectra of organics obtained for fossil and extant specimens were similar (Table 1, Figure 6A), though some peaks were not as well resolved in the fossil. The OH stretching absorbance (Figure 6B) was examined using spectral decomposition analysis with Peakfit®. The OH stretching mode in the fossil is greatly reduced as compared to the extant cuttlebone, and shifted to slightly lower wavenumbers (Table 2). The N-H asymmetric stretching vibration is also shifted to slightly lower wavenumbers.
FTIR spectra of fossil (a) M. mississippiensis and chitin from extant (b) Sepia sp. and (c) Loligo sp. A) All spectra are consistent with beta chitin. B) FTIR spectra of the hydrogen stretching region of a, b and c showing reduction in OH of M. mississippiensis. C) FTIR spectra of a, b and c emphasizing Amide I and Amide II absorbances which are distinctive for beta chitin. D) FTIR spectra of a and b; portions of the Amide III region are missing in the fossil. Overall broadening and loss of structure of the peaks is interpreted as breakdown of chitin chains into shorter fragments.
Peaks at 1649 cm−1 (Amide I, Figure 6C) and 1544 cm−1(Amide II, Figure 6C) in cuttlefish, squid and fossil spectra indicate organics consistent with β-chitin. Closer examination of the region ca. 600–1400 cm−1(Figure 6D) shows the principle change in M. mississippiensis is the loss of peaks in Amide III region. Amide I and II peaks may arise from chitin, but also from proteins, humics or other chemicals. It is parsimonious to assume the presence of chitin-like molecules rather than an admixture of substances displaying a similar spectrum.
IHC has been used successfully to detect and characterize ancient biomolecules such as collagen in birds  and dinosaurs . Because chitin antibodies and IHC have been used to successfully detect chitin in extant organisms , , IHC (Text S1) was used here to further elucidate the specific nature of biomolecules found in M. mississippiensis. Analysis of extant Sepia sp. and fossil M. mississippiensis showed specific antibody binding to chitin with comparable avidity and specificity (Figure 7A,B) indicating some endogenous chitin is retained in the fossil, though its morphology is slightly different from chitin in extant Sepia sp. (Figure 7A,B). Fluorescent signal from antibody-antigen interaction was greater than that seen in negative controls (secondary antibody only; Figure 7C,D). When sections were subjected to digestion by chitinase, immunological signal was either reduced where tissues were completely digested (Figure 6E,F), or enhanced where tissues were not completely digested (Figure 7G,H). This signal enhancement seems contradictory, but enzymes are often used to help unmask antigens – resulting in an enhanced immunological signal. Degradation by chitinase is indicated by the lack of order (as seen in Figure 7A,B). The pattern of enhanced signal is common to both fossil and extant specimens, indicating commonality in chemical make-up. An irrelevant antibody (human testosterone) was applied at identical concentrations and under identical conditions to some sections to verify specificity of the antibody-antigen interaction. Results showed no antigen/antibody binding (Figure 7I,J). IHC analysis showed fluorescence consistent with and specific to chitin in both fossil and extant samples which is consistent with the interpretation that original chitin was preserved in these specimens.
A,C,E,G,I, fossil M. mississipiensis (MGS 1951); B,D,F,H,J, extant Sepia sp. A, B) Fossil/extant incubated in chitin antibody and secondary antibody, show antibody/antigen binding. C,D) Fossil/extant incubated in secondary antibody only, no antibody/antigen binding. E,F) Fossil/extant incubated in chitinase, chitin anti-body and secondary antibody. Tissues have been digested by the chitinase giving a much reduced immunological signal. G,H) Fossil/extant incubated in chitinase, chitin antibody and secondary antibody. Tissues in these samples were not completely digested by the chitinase, eptiopes were unmasked, and show enhanced antibody/antigen retrieval. I,J) Fossil/extant incubated in human testosterone antibody and secondary antibody, shows no antibody/antigen binding.
The initial analysis of fossil M. mississippiensis by multiple methods yielded results that, taken alone, were insufficient to support the presence of a specific organic material. High percentage of phosphorus detected in EDS analysis shows possible preservation of organic material between aragonitic spherulites. Hyperprobe reveals nitrogen clearly unassociated with carbonate minerals, though the overall morphology of de-mineralized M. mississippiensis looks different from extant Sepia sp. FTIR analysis shows presence of amides, and spectra that resembled extant beta chitin. Finally, IHC reveals presence of molecules with specific reactivity to chitin anti-bodies indicating preserved organic material in M. mississippiensis is still recognizable as chitin.
Results of IHC support our interpretation of FTIR data as representative of chitin. Because spectra obtained in FTIR indicate chitin, the lack of a doublet in the Amide I region identifies the fossil material as beta chitin, which is also found in extant mollusks. Differences between spectra of fossil and extant chitin can be used to further illuminate changes in the β- chitin structure of the fossil.
β-chitin is characterized by parallel chains of chitin molecules held together with inter-chain hydrogen bonding . The OH stretching absorbance (Figure 5B), at about 3445 cm−1 in extant chitin, is diminished in the fossil and shifted to lower wavenumbers (Table 2), showing that the specimen is losing OH by an as yet undetermined mechanism. The N-H asymmetric stretching vibration is shifted to slightly lower wavenumbers, showing that it is no longer hydrogen bonding exactly as in extant specimens. Changes in the region 2800–3600 cm−1 indicate that biomolecules have been degraded via disruption of interchain hydrogen bonds.
Examination of the region 800–1500 cm−1 shows that the spectrum of fossil material roughly follows the spectrum of the extant material (Figure 5D). This region has been referred to as the “crystallinity region” for oligosaccharides . Loss of well-defined peaks in the fossil material is ascribed here as a loss of long-range ordering in strands of chitin due to shortening of the chitin strands. Similar changes in FTIR spectra have been observed in the breakdown of chitosan  and the organic components of nacre .
Shortening of strands and loss of hydrogen bonds could explain morphological differences between de-mineralized M. mississipiensis and extant Sepia sp. seen in Hyperprobe (Figure 3A,B) and IHC (Figure 6A,B). In addition, not all pieces of de-mineralized fossil showed amide or lipid peaks (Figure 8). Differences observed in fossil chitin, as compared to extant, could be a result of mechanical destruction, preservational biases or partial chemical degradation within microenvironments of the specimen.
a) spectrum lacks Amide I and Amide II peaks, b) for comparison, spectrum shows the Amide I peak at 1656 cm−1 and Amide II peak at 1545 cm−1.
There is a remote possibility that the organics detected in the fossil are a result of contamination. This is highly unlikely. Chitin is not a common lab contaminant. Fossil specimens used in this study were surface collected, washed in water and stored in climate controlled metal cabinets until they were sent to us in individual sealed plastic bags. None of the samples used for Hyperprobe, FTIR or IHC were preserved using epoxies or other glues. To reduce possible contaminants from extant samples chemical preparations were done in separate labs and FTIR was conducted in a mineralogy lab that has never housed invertebrate specimens. Still, there is a slight possibility that the chitin detected in our samples could a result of extant fungus. This also is unlikely, as we saw no evidence of fungi in SEM. FTIR results showed β-chitin in our samples not γ-chitin found in fungi . Results of IHC were specific for chitin (Figure 7), thus it is unlikely that the amide peaks seen in FTIR (Figure 6, Table 1) are result of contaminating proteins from human or other sources.
Mechanisms for preservation
In vivo inorganic-organic structure of the cuttlebone , in combination with physical and geochemical conditions within the depositional environment ,  and favorable taphonomic factors likely contributed to preservation of organics in M. mississippiensis. Available clays within the Yazoo Clay in conjunction with suboxic depositional environment – may have facilitated preservation of original organics by forming a physical and geochemical barrier to degradation –. One key to the preservation of organic tissues, in particular chitin and chitosan, is cessation of bacterial degradation within environments of deposition. Bacterial breakdown of polymeric molecules is accomplished through activities of both free extracellular enzymes (those in the water column) and ektoenzymes (those on the surface of the microbial cell) such as chitinases , . Chitinases function either by cleaving glycosidic bonds that bind repeating N-acetyl-D-glucosamine units within chitin molecules or by cleaving terminal N-acetyl-D-glucosamine groups –. These enzymes adsorb to the surface of clay particles, which inactivates them –. Strong ions in solution like iron may act in the same manner . Once bound to functional groups within these polymeric molecules, Fe2+ ions prevent specific bond configuration on the active-site cleft of specific bacterial chitinases and prevents hydrolysis , , thus contributing to preservation.
Organic layers within cuttlebones are protected by mineralized layers, similar to collagen in bones, and this mineral-organic interaction may also have played a role in their preservation –. Specimens of M. mississipiensis show preserved original aragonite as well as apparent original organics. These organics appear to be endogenous and not a function of exogenous fungal or microbial activity. Fungi contain the γ form of chitin not the β allomorph found in our samples. Also, SEM analyses shows there is no evidence of tunneling, microbes, or wide-spread recrystallization of the aragonite. Therefore the chitin-like molecules detected in fossil sample are most likely endogenous. Similar to collagen in bone, perhaps, organics could not be attacked by enzymes or other molecules until some inorganic matrix had been removed .
Analysis by Hyperprobe, FTIR and IHC of cuttlebones of the Eocene coleoid cephalopod M. mississipiensis show preservation of degraded original organics consistent with β-chitin. These are oldest known organics consistent with β-chitin (34.36 mya)  and are the first from a fossil marine mollusk. Preservation of organics consistent with β-chitin was possible due to the inorganic/organic construction of cuttlebone and available free Fe2+ ions in suboxic depositional environment, which inhibited microbial or enzymatic degradation. Future work will focus on comparisons with other Eocene cuttlefish and the phylogenetic implications of chitinous structures with regards to the origin cuttlefish.
The authors are indebted to David Dockery III (Mississippi Geological Survey) for the loan of M. mississippiensis, James Bain (NC Fossil Club) for donation of Sepia sp., Mary Schweitzer, Timothy Cleland and Elizabeth Johnson (NC State University) for guidance with chemical analyses, Rainer Martin (University of Ulm) for his donation of chitin antibodies, Stefan Bengtson (Swedish Museum of Natural History) for discussion on EDS data, Steven Singletary (Fayetteville State University) for assistance with Hyperprobe and Janet Edgerton (NCSM) for bibliographic assistance.
Conceived and designed the experiments: PW LD DL RT CC JC WZ. Performed the experiments: PW LD DL RT JC WZ. Analyzed the data: PW LD DL RT CC JC WZ. Contributed reagents/materials/analysis tools: PW LD DL RT CC JC WZ. Wrote the paper: PW LD DL RT CC JC WZ.
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On Tuesday, the rating agency Standard & Poor’s stepped up the pressure on the upcoming EU summit, threatening to downgrade the credit rating of all the euro countries, including Germany. If the economically strongest countries in Europe lose the top rating “AAA,” this would raise the cost of the euro rescue fund, placing a question mark over all previously agreed measures to deal with the debt crisis.
Leading EU representatives were visibly angered by S&P’s move, which they regard as the voice of US financial interests. Euro group chief Jean-Claude Juncker described the decision by Standard & Poor’s as a “knock-out punch for all states that are seeking to reduce their budget deficits.” It was “unfair” and “completely excessive,” Luxembourg’s prime minister said. He was “astonished” that this came just before a crucial EU summit.
Merkel and Sarkozy reacted to the announcement by the rating agency by pledging their complete loyalty to the financial markets. “France and Germany, in full solidarity, confirm their determination to take all the necessary measures, in liaison with their partners and the European institutions, to ensure the stability of the euro area,” they wrote in a joint statement.
But the financial markets will not rest until all social gains have been destroyed, which the European working class won after World War II. In their view, benefits and pensions in Europe are still far too plentiful; wages are too high and working conditions too inflexible; health and education too expensive, and the public sector too bloated. Anything that does not satisfy their insatiable hunger for profit must be cut and eliminated.
They are not satisfied by declarations of intent, they want action. The technocrat-led governments in Greece and Italy have now started this. Earlier this month, the Greek government sacked the first 16,000 public sector employees and drastically cut salaries in state-owned companies. In the coming days, a new austerity budget will be submitted to parliament. The Italian government has fast-tracked pension cuts, which their predecessors tried to implement unsuccessfully for 20 years.
Europe is becoming increasingly reminiscent of the 1930s, when similar drastic austerity measures resulted in mass poverty, dictatorship and war. Even some bourgeois politicians and commentators now make such parallels.
Under the title “The long shadows of the 1930s,” Financial Times columnist Gideon Rachman wrote on November 28: “The risk of a grave economic crisis in Europe is severe. The threats of sovereign-debt defaults and the break-up of the European single currency are rising—and with it, the attendant threats of collapsing banks, popular panic, deep recessions and mass unemployment.... The lesson of the 1930s is that a global depression weakens democracies, leads to the rise of radical new political forces—and, in the process, raises the risk of international conflict.”
The 92-year-old former German Chancellor Helmut Schmidt warned, in a much-publicized speech at the SPD party congress, against Europe relapsing into armed conflicts. He railed against the “globalized banking lobby” and “a few thousand financial traders in the US and Europe, plus some rating agencies that took politically accountable governments in Europe hostage.”
But the answers offered by the critics of Merkel and Sarkozy—regulation of the financial markets, Euro Bonds, unlimited ECB loans, etc.—either contribute to exacerbating the crisis or fail to take economic and political reality into account.
The German Social Democrats and Greens—as well as the British and US governments—call for relieving pressure on the euro by flooding the financial markets with new money from the ECB and the issuing of Euro-bonds.
But this is the very course that the governments had already taken during the 2008 financial collapse, bringing about the current crisis. The transfer of trillions from the state coffers into the banks has contributed significantly to the current debt crisis. And most of this money went into speculative transactions, now directed against the euro and the EU.
Without bringing the financial markets under control and seizing the tremendous assets that have accumulated at the top of society, there can be no solution to the crisis. But none of the establishment parties in Europe is willing or able to take this course. They are all too closely linked with capitalist property relations and their associated privileges.
Conservatives, Social Democrats, Greens and “left” parties agree that there is no alternative to austerity. Even public works and economic stimulus programmes, like those implemented in the 1930s under Franklin D. Roosevelt in the US, are no longer discussed.
International capitalism is in a desperate crisis, which can only be resolved through the mobilization of the European working class on the basis of a socialist programme. The major financial and industrial concerns must be expropriated and placed under democratic control, their enormous resources used to meet social needs rather than to enrich the top one percent. This requires the establishment of workers governments and the United Socialist States of Europe.


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Determination of the physiological and pathological roles of E2F3 in adult tissues02 Oct 2017
While genetically engineered mice have made an enormous contribution towards the elucidation of human disease, it has hitherto not been possible to tune up or down the level of expression of any endogenous gene. Here we describe compound genetically modified mice in which expression of the endogenous E2f3 gene may be either reversibly elevated or repressed in adult animals by oral administration of tetracycline. This technology is, in principle, applicable to any endogenous gene, allowing direct determination of both elevated and reduced gene expression in physiological and pathological processes. Applying this switchable technology to the key cell cycle transcription factor E2F3, we demonstrate that elevated levels of E2F3 drive ectopic proliferation in multiple tissues. By contrast, E2F3 repression has minimal impact on tissue proliferation or homeostasis in the majority of contexts due to redundancy of adult function with E2F1 and E2F2. In the absence of E2F1 and E2F2, however, repression of E2F3 elicits profound reduction of proliferation in the hematopoietic compartments that is rapidly lethal in adult animals.


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Recipe by cookiedog
This is from Fresh Ways with Desserts. A little gift left by a friend after garage "sailing". We thought this recipe was was worth the price of the set of books!EDIT:After the 1* review I eliminated some of the lemon juice. The original recipe called for 1 tsp. of lemon juice in the caramel and 3 tsp. in the flan. The banana gives this flan some texture.
- 1⁄2 cup sugar, plus
- 1⁄3 cup sugar
- 2 teaspoons fresh lemon juice
- 1 cup milk
- 2 eggs
- 1 tablespoon dark rum
- 1 teaspoon pure vanilla extract
- 1⁄4 teaspoon ground cardamom or 1⁄4 teaspoon cinnamon
- 2 -3 bananas, pureed to equal 1 cup
- 2 bananas, peeled and diagonally sliced (optional)
Directions See How It's Made
- Preheat the oven to 325°F.
- Begin by caramelizing a 1-quart souffle dish or a 6-inch-diameter cake pan; In a small heavy-bottomed saucepan, combine 1/2 cup of the sugar, and 3 tablespoons of water. Cook the mixture over medium-high heat until the sugar caramelizes-it will have a rich golden-brown hue. Immediately remove the saucepan from the heat. Working quickly, pour the caramel into the souffle dish or cake pan. Using potholders to protect your hands, tilt the dish in all directions to coat the bottom and about one inch of the adjacent sides. Continue tilting the dish until the caramel has hardened, then set the dish aside.
- To prepare the custard, put the milk into a heavy-bottomed saucepan over medium heat. As soon as the milk reaches a boil, remove the pan from the heat and set it aside. In a bowl, whisk together the eggs and the remaining 1/3 cup sugar, then stir in the rum, vanilla, cardamom or cinnamon, banana puree, and the remaining 2 teaspoons of lemon juice. Stirring constantly to avoid curdling the eggs, pour the hot milk into the banana mixture. Transfer the custard to the caramelized dish.
- Set the custard dish in a small roasting pan and pour enough hot tap water into the pan to come 1 inch up the sides of the custard dish. Bake the flan until a knife inserted in the center comes out clean-20 to 30 minutes. (Take care not to insert the knife so deep that it pierces the caramel coating.) Remove the flan from the hot-water bath and let it cool to room temperature. Put the flan into the refrigerator until it is chilled- about 3 hours.
- To unmold the chilled flan, invert a serving plate over the top of the dish, then turn both over together. The dish should lift away easily; if it does not, turn the dish right side up again and run a small sharp knife around the top of the custard to loosen it. If you like garnish the flan with a ring of banana slices. Cut the flan into wedges and spoon some of the caramel sauce over each one before serving.

domain: food


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I hosed myself when I received the press release for next year’s FLF (Freaks, Luvvies and Fools) wine writer competition last week.
“Writers entering articles for consideration for the short-form prize should submit 6-8 pieces of between 1000 and 1500 words each, written for a column or a blog. Entries for the long-form prize should be between 3000 and 4000 words, and can either be unpublished, or published between 1 March 2013 and 28 February 2014.” There was an embarrassing re-scoping two days later leading to a re-release
“After receiving feedback from a number of people regarding the word count requirements for each of the two categories, the organisers have amended these to the following: Short form: Up to 1000 words; Long form: 1000-4000 words.“
But of course the real problem for the organizers is that they don’t define what a wine writer is. Seems to be anyone who lives in SA and who can write in any one of the 11 official languages. Publication is not a requirement. No wonder Johann Rupert is not interested in print media in SA after being asked to start a new publication by a former editor of defunct Wine magazine:
So rather than a call to boycott the FLF fiasco after the re-appointment of shock-jock and serial hater John Maytham as judge after he royally stuffed up the competition this year, a tsunami of entries should be encouraged from all those lonely hearts and stalkers who fix on wine as a remedy for their social inadequacy issues.
I’m putting my money on whale cottage who produces a minimum of 4000 pieces of flotsam and jetsam a day in an attempt to divert the attention of Google away from customer complaints about his guest houses on the interwebs. Perhaps he will use the R12,500 first prize (way less than half the Du Totiskloof/Standard Bank prize and less even than the Veritas Young Winewriters Prize) to pay a plumber to remove the forests of pubic hair which clog his showers.
Is this really the amateurish freak show a brand like Porcupine Ridge wants to associate itself with?


The Dallas Mavericks and the Los Angeles Clippers will face off Wednesday night in the third game of the Mavericks’ five-game road trip.
This will be Kyrie Irving’s debut in a Mavs uniform after being traded from the Nets, they will look for another win after knocking off the Jazz in their last game. Meanwhile, the Clippers are coming off a road trip in which they went 4-2 and will be looking for their 32nd win of the year in their first game back at home.
We have a great slate of NBA games tonight, make sure you tune in and get access to all the action on fuboTV.
Dallas Mavericks vs. Los Angeles Clippers
- When: Wednesday, February 8
- Time: 10:00 p.m. ET
- TV Channel: ESPN, ESPN Deportes
- Live Stream: fuboTV (watch for free)
NBA Odds and Betting Lines
NBA odds courtesy of Tipico Sportsbook. Odds were last updated Wednesday at 7:00 p.m. ET.
Dallas Mavericks vs. Los Angeles Clippers (-8.5)
Want some action on the NBA? Place your legal sports bets on this game or others in CO and NJ at Tipico Sportsbook.
We recommend interesting sports viewing/streaming and betting opportunities. If you sign up for a service by clicking one of the links, we may earn a referral fee. Newsrooms are independent of this relationship and there is no influence on news coverage.


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Event: B-town Celebs attended Bikram Choudhary's Fitness center launch party held at JW Marriott, Juhu, Mumbai.
Guest List: Jackie Shroff, Manisha Koirala, Anil Kapoor, Sonu Sood, Meghna Naidu, Shivani Kashyap, Karishma Tanna, Chandrachur Singh, Sudhanshu Pandey, Bappi Lahiri, Leena Chandavarkar, Sumit Kumar, Shweta Bhardwaj, Maria Goretti, Moushumi Chatterjee, Bikram
Venue: JW Marriott, Juhu, Mumbai.
HOT and SEXY Bollywood actress Malaika Arora Khan was spotted at Bandra.
Bollywood celebs Karan Johar and Alia Bhatt were spotted at the hospital.
Bollywood actor Ranveer Singh was spotted at Shankar Mahadevan's studio.
Bollywood actor Shahid Kapoor was snapped at Hakkaim Ali Salon in Bandra.
Bollywood actor Suniel Shetty's wife Mana Shetty was spotted at Korner House.

domain: celebrity


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This PPA (Prosthetic Penis Attachment) has a straight shaft and a moderate head and ridges. An elastic strap is included. Available in 6" X 1.5". 5 inches of insertable length. Designed for use with or without an erection.
She loves the extra width and length. She came twice and loved it. Didn't fit my penis perfectly, the tip got a little floppy, but that's okay.Anonymous - June 26 2015


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UPDATED 7:45 PM
From the Boston Turkish website:
We regret to inform you that the 19th Annual Boston Turkish Film and Music Festival scheduled for Thursday, March 19th through April 25, 2020 has been cancelled in response to growing concerns related to the spread of the Coronavirus (COVID-19).
We fully understand that this is disappointing news and we’ll do our best to re-schedule the festival films to fall 2020.
The Boston Turkish Film Festival, which is held every year at the Museum of Fine Arts, is likely a casualty of the decision to close the Museum of Fine Arts for up to 30 days; we’ll try to get more on BTFF’s fate in the coming hours. For now, here’s the statement from the MFA:
Dear MFA Community,
Earlier today you received a message announcing the MFA’s intent to remain open while we closely monitor the COVID-19 outbreak in the US. Due to the rapidly changing situation, the Museum has now decided to close tonight, March 12, at 6 pm and remain closed for up to 30 days. During this time, all Museum events are cancelled or postponed. This decision was made in consultation with other Boston-area museums and sister institutions across the US as a response to ensure the health and safety of our staff and volunteers, visitors, and community.
Given the evolving nature of the outbreak, we will carefully reassess the situation in the coming weeks and share updates as they become available. Please continue to check our website for new information. We appreciate your support and look forward to reopening when it is safe for the public, staff, and volunteers.
Thank you for your continued patience and understanding.
Ann and Graham Gund Director


USB ports stopped working suddenly Asus ROG laptop running Windows 10 - Can't locate drivers. Help!
Problem: Out of the blue last week, my wired mouse stopped working. I unplugged it from the USB port and plugged it back in, and nothing. I then noticed that my external portable USB drive was not showing up. I then checked the mouse and drive in a different computer and both worked, so I realized it was the USB ports. Upon investigation, I opened device manager and found under "universal serial bus controllers" that all three drivers had a yellow yield warning symbol (see attached screen grab) and when I opened the properties on one of them, the "device status" states:
Windows cannot load the device driver for this hardware. The driver may be corrupted or missing. (Code 39)
%hs is either not designed to run on Windows or it contains an error. Try installing the program again using the original installation media or contact your system administrator or the software vendor for support. Error status 0x
Screen shot here: https://drive.google.com/open?id=1T7PA7X2Om9CJyPFGudiXtBwyHzoAG1w3
After googling and reading suggestions, I tried to uninstall the drivers and restart the computer, only to find they came right back with the same error warning. I also tried in vain to find an updated driver (as the driver date shows 6/21/2006--over 12 years old!!!) but I couldn't find one OR Im not looking in the right place.
I did try a fresh install of windows by going through the Recover > Reset this PC option, but I get a notice that states "Could not find the recovery environment". Upon investigation, apparently I need to load something onto a USB drive and proceed that way, but that wont work specifically because the original problem has to do with sudden non functioning USB ports.


MLGQ-B series double body automatic pneumatic rice huller is new generation rice hulling machine which developed by our company. It is a automatical air pressure rubber roller husker, mainly used for paddy husking and separation. Is with characteristics such as high automation, large capacity, fine effect, and convenient operation. It can satisfy the requirement of mechatronics of modern rice milling equipment, necessary and ideal upgrade product for large modern rice milling enterprise in centralization production.
1. Double body structure, double capacity but less area occupy.
2. Opening for feeding gate and pressure between the rubber rollers are automatically controlled by pneumatic
3. The feeding flow and air volume adjusted by adjustable handle.
4. Different speed of double rollers is interchanged by gear shift, easy to operate.
5. Continuous voltage regulation, uniform pressure. Continously regulate the pressure of roller engagement more
uniformly than by weight balanced, reduced the broken rate and enhance the exuviating effect.


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Akanksha Puri may not have been a part of any film but she keeps creating a ruckus on social media. At this time, one of her posts has surfaced which is enough to surprise you. You are going to be surprised to see her style in these pictures going viral.
Akanksha Puri has raised the temperature by standing on the steps of the pool in just a bra and panty. Obviously, you are going to be surprised to see her sexy figure. Whoever takes a look at her stops there.
Akanksha has posted many pictures one after the other and people are surprised to see them. It is noteworthy that Akanksha Puri has won the hearts of people with her sexy looks. Along with these pictures, Akanksha has written the caption, “Splash Splash.” After this, people are continuously commenting.
Reactions of Fans
One wrote, “Someone please call the fire brigade.” Another wrote, “Wow, what a scene, man.” One wrote, she is hottie. One wrote, “This also showed her status.” Another said, “Very good.” I can’t control my mind. My mind is spinning.”
One wrote, “Very sexy, reminds me of 2008.” People are continuously reacting in this way. However, Akanksha has not given any response on social media.
Whom did you bring into the discussion?
During the task in Bigg Boss OTT, Akanksha lip-locked with Jad Hadid for 30 seconds and this kissing video created a stir. Salman Khan later scolded both of them.
Talking about the journey of the Bigg Boss house, JD Hadid was very much in love with Akanksha Puri from the very first day. Ever since Akanksha entered the house with Palak Purswani, JD was paying all her attention to Akanksha.


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Still "Feelin' Groovy" – Reuter Center Singers to Present '60s Folk Songfest
Tue, 04/26/2011 - 11:18am
The Reuter Center Singers will present a '60s Folk Songfest at 3 p.m. Sunday May 15, at UNC Asheville's Reuter Center. A chorus of more than 50 voices, the Reuter Center Singers stage spring and fall concerts at the North Carolina Center for Creative Retirement, and perform in many other community venues, including Biltmore House, the Veterans' Administration Hospital, churches and retirement homes.
The '60s Folk Songfest will include Woody Guthrie's "This Land is Your Land," music recorded by the Kingston Trio and many others, and anthems that are reminders of the civil rights and peace movements, like "We Shall Overcome" and "Where Have All the Flowers Gone."
"I was surprised that the music seems just as important now as it did when I was a young girl," said chorus-member Lynda Feldman. "When I sing the music again, I'm re-inspired." Chorus Director Chuck Taft says the folk songs of that era "speak to all people no matter where they stand on the political spectrum. These are great songs about people working hard and their struggles through times of trouble and uncertainty."
The concert is free and open to the public; donations are appreciated. For more information, call UNC Asheville's North Carolina Center for Creative Retirement at 828.251.6140.


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Located in the heart of Napa, only minutes from the downtown nightlife or wine tasting in the valley. September is the best time of year to experience Napa Valley, just as the vines turn gold and burgundy and the Harvest Crush begins! This vacation cottage includes all indoor amenities you could want and the resort it resides in also gets you access to the pool, spa, gym, sauna, rec room, and more! Come experience Napa in your very own cozy cottage directly on the banks of the Napa River!
(Još) nema recenzija
Ovaj smještaj nema recenzija. Odsjednete li u njemu, ovdje će se prikazivati vaša recenzija.

domain: geography


text: Purification of recombinant M. tuberculosis BCAT. E. coli BL21(DE3) CodonPlus-RIL cells were induced with IPTG and prepared as described in the Materials and Methods section. The cell lysate was separated by centrifugation into pellet (P) and supernatant (S) fractions. The supernatant was loaded onto an Ni2+-charged metal ion affinity column and flow through (F), 80 mM imidazole (W), and 800 mM imidazole (E) fractions were collected. Aliquots of each fraction were analysed on a 10% polyacrylamide gel under reducing conditions. Lane (M) contains molecular mass markers (units in kDa).


Kentucky NORML sponsors exhibit at Capitol in support of medical cannabis legalization
Month: December 2022
As a patient and consumer focused advocacy organization, NORML prioritizes efforts in favor of state-level legislation that seeks to achieve the following goals. We are also keeping an eye on the following states for 2023 advancement.
“It is time for politicians and others to stop assessing cannabis through the lens of ‘what we don’t know’ and instead start engaging in evidence-based discussions about marijuana and marijuana reform policies that are indicative of all that we do know.”
District of Columbia: Council Members Advance Legislation to Expunge Tens of Thousands of Marijuana Convictions
Prior to 2015, the District had one of the nation’s highest per capita arrest rates for marijuana-related offenses — averaging one arrest every two hours.
What we do now will determine how successful we are next year.
“Democrats’ failure and the GOP’s continued resistance to any progress is out of step with voters’ opinion, is bad politics, and most importantly, it is bad public policy.”
Monitoring the Future has released their 2022 findings regarding cannabis use rates for 8th, 10th, and 12th grade students. The study found that rates for each cohort were largely unchanged from last year.
Provisions in the bill provide pathways so that those with misdemeanor marijuana convictions (and/or those convicted of most misdemeanors and fourth- or fifth-degree felonies) can petition the courts to have their records sealed.


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This page contains Pokemon Battle Trozei Unlockables for 3DS called "Zone 6 Stage 5" and has been posted or updated on Apr 8, 2014 by ilb322. Let us know what you think of this Unlockable by thumbs voting.
- Developer: Genius Sonority Inc.
- Publisher: Nintendo
- Genre: Puzzle Matching
- Release Date: Mar 20, 2014
- ESRB: Everyone
Zone 6 Stage 5
Defeat Darkai (Stage 4) with a single hit.
Stuck on this game? Set a specific, clearly defined question and let others answer your question or view answers to previously asked questions. If you think you are an Pokemon Battle Trozei expert then please try to help others with their questions.

domain: entertainment


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SPLASH Cuisine : Apparel Location : Asmak Mall Contact No : +974 44131927 Multi-brand store with international & in-house designed brands Headquartered in Dubai, Splash is Middle East's largest fashion retailer a..
According to this year’s Mercer cost of living survey, Doha has been ranked the cheapest city in the gulf region for expats. Among the listed 209 cities in the report, Doha city is ranked 130 this year coming down from 109 as compared to last y..
Top oil exporter Saudi Arabia has raised the August official selling prices (OSPs) of all crude grades it sells to Asia, the country's state oil producer Aramco (2222.SE) said on Tuesday. It set the August OSP for the flagship Arab light crude..
DUBAI - Dubai's largest listed developer Emaar Properties (EMAR.DU) expects to buy out minority shareholders of Emaar Malls (EMAA.DU) and delist the business by year-end, a spokesperson said on Tuesday. The all-share deal to make Emaar Malls a..
GE Aviation and Safran have made a future-defining announcement for the two aerospace companies: the CFM RISE (Revolutionary Innovation for Sustainable Engines) Technology Demonstration Program. The RISE program targets more than 20 percent ..
Usmanullah Mir Dill Khan has been doing social service for a long time and has helped several other nationalities. Humanity has no boundaries. And the UAE is the best place to witness this. Despite disagreements between India and Pakistan, citizen..
The suspension also includes Pakistan, Bangladesh, Sri Lanka, Oman, and Nepal The Philippines has extended the travel ban on travellers from UAE and six other countries until July 31 to prevent the spread of the highly-transmissible ‘Delta&r..
According to new information from travel analytics firm Forward Keys, Qatar's Hamad International Airport (HIA) has overtaken Dubai as the Middle East's busiest airport. HIA has earned this position by adhering to COVID-19 regulations and ..
The enhanced rain is created using drone technology that unleashes electrical charges into clouds in order for them to clump together and form precipitation The monsoon-like downpour drenches a busy highway, causing tricky driving conditions for t..
Afghan Women Speaks on Unyielding Hope of Restoring Their Homeland
QF to Host Education-Focused Virtual Events at United Nations General Assembly and Global Goals Week
This Manpower Firm in Doha Guides You in Hiring Nurse for Your Hospital
Qatar SC wins HH the Amir’s Table Tennis Cup 2020-2021 season
Pfizer: Know all about the Covid-19 vaccine and its usage in Qatar
FIFA’22 over Covid-19: Qatar limbers up for the World Cup amidst a worldwide pandemic
Top 5 Art Galleries You Must Explore in Qatar
The Expert Outsourcing Service in Qatar That Guides You in Employing a Qualified Graphic Designer


– A Nigerian painting has sold for a record breaking price
– The painting Tutu sold for a whooping N600m– It was made by well-regarded Nigerian artist Ben Enwonwu and it was previously reported missing
A Nigerian painting that was said to be missing for decades and was finally been found in a London home has been sold for a record breaking amount.
The painting of Adetutu ‘Tutu’ Ademiluyi, by Ben Enwonwu, recently went under the hammer at Bonhams auction house with bidders in Lagos allowed to participate for the first time.
Tutu is part of a set of three paintings created by Enwonwu around 1973, just a few years after Nigeria’s civil war ended. Enwonwu was reportedly walking in the Nigerian countryside when he met a beautiful young woman whom he just had to paint. The woman Tutu was a princess of Ife, in present day Osun state.
A worker at Bonhams auctioneers poses next to Tutu, by Nigerian artist Ben Enwonwu (Image courtesy Reuters/Peter Nicholls)
According to qz.com, the painting sold for a record $1.68million (N600m) to a bidder on the telephone during Bonhams’ Africa Now auction on February 28, 2018. Another Enwonwu painting, Negritude, sold for $140,000 (N55million) at the auction, their sales overshadowing the other works in the lot.
Award winning novelist Ben Okri described the painting as Africa’s Mona Lisa. “It amounts to the most significant discovery in contemporary African art in over fifty years. It is the only authentic Tutu, the equivalent of some rare archaeological find. It is a cause for celebration, a potentially transforming moment in the world of art,” he said.