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Patent application title: MULTIMERS OF S. SOLFATARICUS SINGLE-STRANDED DNA-BINDING PROTEIN AND METHODS OF USE THEREOF
Cynthia A. Haseltine (Davis, CA, US)
Stephen C. Kowalczykowski (Davis, CA, US)
REGENTS OF THE UNIVERSITY OF CALIFORNIA
IPC8 Class: AC12Q168FI
Class name: Measuring or testing process involving enzymes or micro-organisms; composition or test strip therefore; processes of forming such composition or test strip involving nucleic acid with significant amplification step (e.g., polymerase chain reaction (pcr), etc.)
Publication date: 2012-03-08
Patent application number: 20120058478
The invention provides multimers of S. solfataricus ssDNA binding protein
that bind single stranded DNA. The multimers are robust and stable
reagents for use in PCR and other techniques for engineering DNA. The
invention further provides methods for performing nucleic acid
amplification and engineering using the multimers.
1. An isolated multimer, wherein each unit of said multimer has at least
70% sequence identity to SEQ ID NO:1, and wherein said multimer binds
single stranded DNA.
2. An isolated multimer of claim 1, wherein each unit of said multimer has at least 80% sequence identity to SEQ ID NO:1.
3. An isolated multimer of claim 1, wherein each unit of said multimer has at least 90% sequence identity to SEQ ID NO:1.
4. An isolated multimer of claim 1, wherein each unit has the sequence of SEQ ID NO:1.
5. An isolated multimer of claim 1, wherein said multimer is a tetramer.
6. A method of performing nucleic acid amplification, said method comprising contacting a single stranded DNA with a multimeric protein, wherein each unit of said multimeric protein has at least 70% sequence identity to SEQ ID NO:1, and wherein said multimeric protein binds single stranded DNA.
7. A method of claim 6, wherein each unit of said multimeric protein has at least 80% sequence identity to SEQ ID NO:1.
8. A method of claim 6, wherein each unit of said multimeric protein has at least 90% sequence identity to SEQ ID NO:1.
9. A method of claim 6, wherein each unit of said multimeric protein has the sequence of SEQ ID NO:1.
10. A method of claim 6, wherein said nucleic acid amplification is selected from the group consisting of polymerase chain reaction, ligase chain reaction, transcription-based amplification system, and self-sustained sequence replication system.
11. A method of claim 10, wherein the method of nucleic acid amplification is polymerase chain reaction.
12. A method for performing nucleic acid engineering, comprising contacting single stranded DNA with a multimeric protein, wherein each unit of said multimeric protein has at least 70% sequence identity to SEQ ID NO:1, and wherein said multimeric protein binds single stranded DNA.
13. A method of claim 12, wherein each unit of said multimeric protein has at least 80% sequence identity to SEQ ID NO:1, and wherein said multimeric protein binds single stranded DNA.
14. A method of claim 12, wherein each unit of said multimeric protein has at least 90% sequence identity to SEQ ID NO:1, and wherein said multimeric protein binds single stranded DNA.
15. A method of claim 12, wherein each unit of said multimeric protein has the sequence of SEQ ID NO:1, and wherein said multimeric protein binds single stranded DNA.
16. A method of claim 12, wherein said nucleic acid engineering is selected from the group consisting of PCR-based DNA sequencing, recombination mediated cloning, PCR-mediated gene replacement, PCR-mediated recombination, RT-PCR cDNA synthesis, and in vitro sequence mutagenesis.
CROSS-REFERENCES TO RELATED APPLICATIONS
 This application is a continuation of U.S. patent application Ser. No. 10/386,575, filed Mar. 11, 2003, the entire contents of which is incorporated herein by reference.
REFERENCE TO A "SEQUENCE LISTING," A TABLE, OR A COMPUTER PROGRAM LISTING APPENDIX SUBMITTED ON A COMPACT DISK
 NOT APPLICABLE
FIELD OF THE INVENTION
 This invention relates to single stranded DNA binding proteins that are robust reagents for use in nucleic acid amplification reactions.
BACKGROUND OF THE INVENTION
 Single-stranded DNA (ssDNA) binding proteins (SSBs) are essential in most intracellular interactions that involve DNA, including replication, repair, and recombination (Kowalczykowski, S. C. et al., Microbiol Rev 58:401-465 (1994); Lohman, T. M. et al., Annu Rev Biochem 63:527-570 (1994) ("Lohman 1994")). Homologues of this class of proteins were identified in all three domains of life, as well as in viral genomes (Chedin, F. et al., Trends Biochem Sci 23:273-277 (1998) ("Chedin 1998"); Iftode, C. et al., Crit Rev Biochem Mol Biol 34:141-180 (1999); Kelly, T. J. et al., Proc Natl Acad Sci USA 95:14634-14639 (1998); Kowalczykowski, S. C. et al., Single-stranded DNA binding proteins. in The Enzymes. Boyer, P. D. (ed.) New York: Academic Press, pp. 373-442 (1981); Lohman 1994; Wold, M. S. Annu Rev Biochem 66:61-92 (1997)). Despite the lack of strong homology at the amino acid level, preservation of both structural and domain organization suggests that SSBs are derived from a common evolutionary ancestor (Chedin 1998; Pfuetzner, R. A. et al., J Biol Chem 272:430-434 (1997); Raghunathan, S. et al., Nat Struct Biol 7:648-652 (2000)). While functionally equivalent, eubacterial SSB and the eukaryotic version, RPA, have distinctly different quaternary structures. In eubacteria, SSB is encoded by a single gene and the active form of the protein is a homotetramer in which each monomer provides one ssDNA-binding domain (Lohman 1994). In eukaryotes, the RPA complex from both humans and yeast is composed of three distinct subunits which together provide a total of four ssDNA-binding domains (Brill, S. J. et al., Mol Cell Biol 18:7225-7234 (1998)).
 Archaea are a separate group of organisms distinguished from the eubacteria through 16S rDNA sequence analysis. These prokaryotes are further subdivided into three diverse groups named the crenarchaeota, the euryarchaeota, and the korarchaeota (Barns, S. M. et al., Proc Natl Acad Sci USA, 93:9188-9193 (1996); Woese, C. R. et al., Proc Natl Acad Sci USA 87:4576-4579 (1990); Woese, C. R. et al., Proc Natl Acad Sci USA 74:5088-5090 (1977)). Only members of the crenarchaeal and euryarchaeal groups, however, have been cultivated. Genomic studies suggest a significant evolutionary division between metabolic and informational processes in archaea. While most intermediary metabolic processes strongly resemble those observed in eubacteria, genomic informational processes are generally thought to be more closely related to those found in eukaryotes (reviewed in (Doolittle, W. F. et al., Curr Biol 8:R209-211 (1998))).
 Archaea utilize eukaryotic B-type DNA polymerases for replication, and their ribosomal proteins, as well as translation initiation factors, are remarkably eukaryotic. The recent identification of archaeal snoRNA genes reveals an unexpected eukaryotic connection (Omer, A. D. et al., Science 288:517-522 (2000)). Transcription also involves eukaryotic protein homologues, but the discovery of multiple TBP and TFB proteins in halophiles hints at a unique archaeal transcription mechanism (Baliga, N. S. et al., Mol Microbiol, 36:1184-1185 (2000)). Examination of archaeal recombination proteins suggests a definite similarity with eukaryotes. The archaeal DNA strand exchange protein RadA is more similar to its eukaryotic counterpart, Rad51 protein, than to the eubacterial RecA protein, both at the amino acid level (Sandler, S. J. et al., J Bacteriol 181:907-915 (1999)) and at the biochemical level (Seitz, E. M. et al., Genes Dev 12:1248-1253 (1998)). The associated RadB protein is proposed to serve as a simpler archaeal version of eukaryotic Rad55/57 protein (DiRuggiero, J. et al., J Mol Evol 49:474-484 (1999); Komori, K. et al., J Biol Chem 275:33782-33790 (2000); Rashid, N. et al., Mol Gen Genet 253:397-400 (1996)), and archaeal Holliday junction resolvase protein characterization suggests they may also be eukaryotic in nature (Komori, K. et al., Proc Natl Acad Sci USA 96:8873-8878 (1999); Kvaratskhelia, M. et al., J Mol Biol 297:923-932 (2000)).
 Recent descriptions of archaeal SSB homologues from the euryarchaeal branch of the archaeal domain demonstrate their amino acid sequences are more similar to eukaryotic RPA than to eubacterial SSB (Chedin 1998; Kelly, T. J. et al., Proc Natl Acad Sci USA 95:14634-14639 (1998)). However, these archaeal proteins maintain multiple ssDNA-binding domains within one or just a pair of polypeptides and therefore, are expected to function as monomers or heterodimers rather than as a homotetramer (as does E. coli SSB). It was proposed that these archaeal RPA homologues are evolutionarily related to eukaryotic RPA through gene duplication and recombination events (Chedin 1998).
 Genome sequencing of several archaeons simplified molecular analysis in these organisms. While a number of euryarchaeal genome sequences have been determined, to date the only publicly available crenarchaeal genome sequences are that of Aeropyrum pernix (Kawarabayasi, Y. et al., DNA Res 6:83-101:145-152 (1999)) and Sulfolobus solfataricus (She, Q. et al., Proc Natl Acad Sci USA 98:7835-7840 (2001)). In 2001, Wadsworth and White described the identification of an ssDNA binding protein from S. solfataricus. Wadsworth and White, Nuc Acids Res 29(4):914-920 (2001).
BRIEF SUMMARY OF THE INVENTION
 This invention provides isolated multimers, wherein each unit of said multimer has at least 70% sequence identity to SEQ ID NO:1, and wherein the multimer binds single stranded DNA. In some embodiments, each unit of the multimer has at least 80% sequence identity to SEQ ID NO:1. In other embodiments, each unit of the multimer has at least 90% sequence identity to SEQ ID NO:1. The invention further provides embodiments wherein each unit has the sequence of SEQ ID NO:1. In some preferred embodiments, the multimer is a tetramer.
 In another important group of embodiments, the invention provides methods of performing nucleic acid amplification, said method comprising contacting a single stranded DNA with a multimeric protein, wherein each unit of the multimeric protein has at least 70% sequence identity to SEQ ID NO:1, and wherein said multimer binds single stranded DNA. In some of these embodiments, each unit of the multimeric protein has at least 80% sequence identity to SEQ ID NO:1, while in others, each unit of the multimeric protein has at least 90% sequence identity to SEQ ID NO:1. In some embodiments, each unit of the multimeric protein has the sequence of SEQ ID NO:1. The nucleic acid amplification can be, for example, polymerase chain reaction, ligase chain reaction, transcription-based amplification system, and self-sustained sequence replication system. In some embodiments, the method of nucleic acid amplification is polymerase chain reaction.
 The invention further provides methods for performing nucleic acid engineering, comprising contacting single stranded DNA with a multimeric protein, wherein each unit of the multimeric protein has at least 70% sequence identity to SEQ ID NO:1, and wherein said multimer binds single stranded DNA. In some of these embodiments, each unit of the multimeric protein has at least 80% sequence identity to SEQ ID NO:1, while in others, each unit of the multimeric protein has at least 90% sequence identity to SEQ ID NO:1. In some embodiments, each unit of the multimeric protein has the sequence of SEQ ID NO:1. The nucleic acid engineering can be, for example, PCR-based DNA sequencing, recombination mediated cloning, PCR-mediated gene replacement, PCR-mediated recombination, RT-PCR cDNA synthesis, and in vitro sequence mutagenesis.
BRIEF DESCRIPTION OF THE DRAWINGS
 FIG. 1. Alignment of ssDNA-binding domain protein sequences. Crenarchaeal sequences are aligned with the first ssDNA-binding domains of M. jannachii RPA (SEQ ID NO:5), S. cerevisiae RPA70 (SEQ ID NO:6), and H. sapiens RPA70 (SEQ ID NO:7). Identical residues are shaded black and conserved residues are shaded gray. The * symbol indicates residues identified in the H. sapiens RPA70 that interact with DNA (Bochkarev, A. et al., Nature, 385:176-181 (1997)), while an x indicates residues that are identical between the two crenarchaeal sequences. The consensus is shown beneath the alignment. Sequence accession numbers are as follows: S. solfataricus SSB (portion shown in Figure is SEQ ID NO:3), SSO2364; A. pernix SSB (portion shown in Figure is SEQ ID NO:4), gi5105001; M. jannachii RPA, MJ1159; S. cerevisiae RPA70, gi6319321; H. sapiens RPA70, gi1350579.
 FIG. 2. Schematic representation of the domainal architecture of ssDNA-binding proteins in all three domains of life. Homologous DNA-binding domains are represented by shaded boxes and the location of the zinc-finger motif is indicated. A summary of the attributes of each type of ssDNA-binding protein is represented in the boxes. A range of percentage similarities for proteins from each domain to the first single-stranded DNA-binding domain of S. cerevisiae RPA70 were determined using the BestFit program and are indicated.
 FIG. 3. SDS-PAGE of the purified SsoSSB protein. Samples were subjected to SDS-PAGE and gels were stained with Coomassie brilliant blue 8250. The samples loaded were uninduced crude cell sonicate (lane 1, 100 μg protein); induced crude cell sonicate (lane 2, 100 μg protein); heat-treated clarified sonicate (lane 3, 80 μg protein); pooled ssDNA-cellulose fractions (lane 4, 15 μg protein); and concentrated Resource Q fractions (lane 5, 15 μg protein). The arrow indicates the position of the SsoSSB protein.
 FIG. 4 consists of FIGS. 4A and 4B, showing gel filtration chromatography of SsoSSB protein. FIG. 4A. Elution of purified SsoSSB protein relative to molecular weight standards: BSA (66 kDa), carbonic anhydrase (29 kDa), and cytochrome C (12.4 kDa), which are represented by closed squares. Ve/Vo, elution volume divided by void volume. FIG. 4B. A representative elution profile for purified SsoSSB protein. Closed squares represent optical density at 280 nm (OD280) for the elution volume indicated.
 FIG. 5. Gel mobility-shift analysis of SsoSSB protein binding to ssDNA. Increasing concentrations of protein (0.04 μM to 20 μM) were added to a constant concentration of 63-mer oligonucleotide (10 μM nucleotides).
 FIG. 6 consists of FIGS. 6A and 6B, and shows that overexpression of SsoSSB protein rescues the lethal phenotype of an E. coli ssb-1 mutation. Both Figures: KLC789 cells containing the pTara arabinose-inducible T7 expression plasmid, and either pET21a (circles) or the SsoSSB expression vector (squares) were grown at 30° C. in either arabinose (closed symbols) or glucose (open symbols). Cultures were shifted to 43° C. at the point indicated by the arrow. FIG. 6A. Optical densities were monitored spectrophotometrically at 600 nm. FIG. 6B. Colony forming units (cfu) were determined by plating in triplicate and the points shown are averages of the replicates.
 FIG. 7 consists of FIGS. 7A and 7B and shows that SsoSSB protein stimulates DNA strand exchange by E. coli RecA protein. FIG. 7A. A schematic representation of the formation of nicked circular dsDNA and joint molecules from starting substrates. FIG. 7B. Photo of gel. Lane 1, no protein; lane 2, RecA protein; lane 3, RecA protein and SsoSSB protein; lane 4, RecA protein and E. coli SSB protein. The abbreviations are: JM, joint molecules; NC, nicked circular dsDNA; DS, dsDNA; and SS, ssDNA.
 FIG. 8 consists of FIGS. 8A and 8B. FIG. 8A sets forth the amino acid sequence of SsoSSB protein. FIG. 8B sets forth the nucleotide sequence encoding SsoSSB protein.
 The crenarchaeon S. solfataricus is a hyperthermophic aerobe that grows in sulfur hot springs. Its optimal growth conditions are temperatures of 70-90° C. and pH levels from 2-4. The entire genome of the organism was sequenced and published in 2001. She et al., "The complete genome of the crenarchaeon Sulfolobus solfataricus P2," Proc. Natl. Acad Sci (USA) 98:7835-7840 (2001). In 2001, Wadsworth and White reported the identification of a single-stranded DNA (ssDNA) binding protein (SSB) from S. solfataricus. Wadsworth and White, Nuc Acids Res 29(4):914-920 (2001). The work from this laboratory indicates that the SSB is present as a monomer.
 The S. solfataricus ssDNA binding protein ("SsoSSB protein") consists of 148 amino acids with 47% identity and 69% similarity (that is, that the residues are either identical or conservative substitutions for one another) to the SSB of another crenarchaeon, A. pernix. The amino acid sequence of SsoSSB (SEQ ID NO:1) is shown in FIG. 8A, and is available in the National Center for Biotechnology Information Entrez database under accession numbers NP--343725 and AAK42515.
 Surprisingly, it has been discovered that the monomers of S. solfataricus ssDNA binding protein ("SsoSSB protein") associate with one another to form a complex, referred to herein as "multimeric protein" or a "multimer") in solution and it is the complex, or multimeric protein, that is functional in binding ssDNA. The multimeric proteins are, however, composed of monomers of the SsoSSB protein. The multimers are comprised of multiples of 2. It is believed that the multimers are not composed of more than 24 monomers of SsoSSB, and are more commonly composed of 12 or fewer. Data from the studies reported in the Examples indicates that SsoSSB is present in solution primarily as dimers and tetramers, with tetramers being the prevalent multimeric form present. The most active form of the protein is therefore a homotetramer in which each monomer provides one ssDNA-binding domain. While S. solfataricus SSB has no sequence homology to the E. coli SSB protein, it therefore physically acts more like the SSB found in eubacteria, which multimerize, than like those found in eukaryotes and the SSB of other archaeons identified to date.
 Accordingly, the present invention provides multimers comprising monomers of single stranded DNA binding protein of S. solfataricus or of defined variations of the SsoSSB protein that retain the ability to bind ssDNA. In preferred forms, the multimer is a dimer (that is, an assembly of two monomers) or a tetramer (that is, an assembly of four monomers), with the tetramer form being the most preferred. The multimers function to bind ssDNA.
II. Uses of the ssDNA-Binding Proteins of the Invention
 Members of the Archaea typically live in conditions of extreme heat, pH, or salt concentrations. Thus, they offer a source of enzymes and other proteins which can be useful reagents in assays and other commercial reactions. As noted above, the crenarchaeon S. solfataricus grows optimally at temperatures between 70-90° C. Thus, its proteins are particularly adapted for use at temperatures which are high for reagents originating from biological sources. More specifically, the DNA replication of S. solfataricus takes place at high temperature. But, the multimers are active over a wide range of temperatures, and can be used at temperatures as low as 37° C. to as high as 65° C. and even as high as 90° C.
 During polymerase chain reaction (PCR), this activity permits DNA polymerase to replicate more of the DNA template strand in each PCR cycle than would be replicated in the absence of the protein, thereby increasing yield. Moreover, temperature-resistant proteins such as SsoSSB protein multimers are not inactivated by the temperature cycling which is part of the PCR process, and thus do not have to be replaced before the next reaction can proceed. This enhances the ability to automate the procedures. Thus, use of heat-resistant ssDNA-binding proteins, like the multimers provided here, not only increases the yield of each PCR cycle, but also permits automation of the overall process and the speed with which cycles can be conducted.
 Additionally, archaeal proteins are much more stable than most eukaryotic and bacterial proteins. For example, SSB protein from E. coli must be stored at -80° C. If refrigerated, it loses some or all of its activity within a month and at room temperature, it loses some or all of its activity within 72 hours. By contrast, SsoSSB retains its activity at room temperature for at least three weeks, and can be refrigerated for over a year without loss of activity. The stability of the protein therefore makes it convenient for use even in protocols in which high temperatures are not required.
 SsoSSB protein multimers are therefore robust reagents useful for a variety of biotechnical applications involving amplification or engineering of nucleic acids, or both. The multimers are expected to be useful in a number of such techniques well known in the art, such as PCR, ligase chain reaction, transcription-based amplification system, self-sustained sequence replication system, PCR-based DNA sequencing, recombination mediated cloning, PCR-mediated gene replacement, PCR-mediated recombination, reverse transcriptase (RT)-PCR cDNA synthesis, and in vitro sequence mutagenesis. For convenience, techniques which employ the binding of ssDNA in the course of manipulating nucleic acids, such as PCR-based DNA sequencing, recombination mediated cloning, PCR-mediated gene replacement, PCR-mediated recombination, reverse transcriptase (RT)-PCR cDNA synthesis, and in vitro sequence mutagenesis, are sometimes referred to herein as "nucleic acid engineering."
III. Modifications of S. solfataricus ssDNA Binding Protein Multimers.
 It is understood that the S. solfataricus ssDNA binding protein can be modified and still retain the desired robustness and ssDNA binding function. In some embodiments, the amino acid sequence has at least 70% identity to that of SEQ ID NO:1. In other embodiments, the amino acid sequence has 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or even 99% identity to SEQ ID NO:1 (with each increasing percentage of identity being more preferred), and retains the ability to bind ssDNA.
 It should be noted that the monomers composing a particular multimer need not be exact duplicates of one another. Thus, while a dimer or a tetramer of the invention is for convenience considered a homodimer or a homotetramer, the monomers of the dimer or tetramer may not be precisely identical. In a given tetramer, for example, one monomer might have the native sequence of SEQ ID NO:1, the second monomer might have a conservative substitution compared to the native sequence (SEQ ID NO:1) and the third and fourth monomers might have only 80% and 90% sequence identity, respectively, to the native sequence. Or, all four monomers of the tetramer might be close to the sequence of SEQ ID NO:1, but each might have several conservative substitutions of residues of SEQ ID NO:1. Or, all four monomers might have the same two conservative substitutions and otherwise have the sequence of SEQ ID NO:1, or all four might have the sequence of native SEQ ID NO:1, in which case all the monomers are identical to one another. Whatever the exact composition of the individual monomers, however, it is important is that the multimer they form retains the desired ability to bind ssDNA.
 Persons of skill are also aware that ssDNA binding proteins typically contain a motif known as an oligonucleotide/oligosaccharide binding ("OB")-fold". OB-fold proteins are a superfamily of proteins having common structural features; both the superfamily and the common structural features are well known in the art. See, e.g., Callebaut and Mornon, Biochem J 321:125-32 (1997). Williamson et al., Biochemistry 33:11745-59 (1994) summarize some of these features: "[t]he common structural features include the number of beta-strands and their arrangement, the beta-barrel shear number, an interstrand hydrogen bond network, the packing of the hydrophobic core, and a conserved beta-bulge." One feature of ssDNA binding, OB-fold proteins is the presence of a channel in the protein so sized as to permit binding of ssDNA along the channel. As noted by Bochkarev et al., Nature 385:176-81 (1997): "[t]he ssDNA lies in a channel that extends from one subdomain to the other." For ease in visualization, the protein channel is sometimes described in the art as similar in conformation to a hand curled around a glass.
 SsoSSB protein is a monomer, which contains an OB-fold between residues 32-71. The monomers assemble into a multimer with structural features typical of OB-fold proteins which bind ssDNA. Multimers of SsoSSB proteins form a channel so sized as to permit binding of the ssDNA along the channel. The carboxyl terminus of the protein monomers also contains a number of acidic residues.
 FIG. 1 shows an alignment of SsoSSB protein with other OB-fold ssDNA binding proteins. As noted in the Description of FIG. 1, the SsoSSB residues shown on a black background are identical among these proteins. These residues can be assumed to be important for protein function and their substitution in an SsoSSB monomer is therefore generally less favored. As also noted in the Description of FIG. 1, residues shown on a gray background are conservative substitutions among the proteins. Thus, it is expected that other conservative substitutions of these residues in an SsoSSB monomer will likely result in a functional ssDNA binding proteins multimer. The residues shown in FIG. 1 on a normal, white background are not conserved among the various ssDNA binding proteins aligned in the Figure; these residues can generally undergo substitution. In preferred embodiments, the substitutions are conservative substitutions. Substitutions can also generally be made outside of the OB-fold region defined by residues 32-71. Any particular substitution can be readily tested, for example by the assays set forth in the Examples, to confirm that the substitution does not decrease the ability to bind ssDNA below any particular degree chosen by the practitioner.
 Preferably, a multimer containing the modified protein retains at least 50% of the ability of a multimer of native S. solfataricus SSB to bind DNA. More preferably, a multimer containing the modified protein has at least 60%, 65%, 70% 75%, 80%, 85%, 90%, 95% or even more of the ability of a multimer of native S. solfataricus SSB to bind ssDNA, as measured in such assays. Gel-shift assays provide especially convenient methods of determining the degree to which any particular modified SsoSSB protein multimer retains the ability of a native SsoSSB protein multimer to bind single stranded DNA. Many other such assays are, however, known in the art and can be used at the practitioner's choice. For example, the multimers can be permitted to bind ssDNA and the fluorescence of the proteins examined by spectrophotometry. Higher degrees of binding are detected by decreased fluorescence as more amino acids of the proteins are blocked by the DNA.
 Although the discussion above refers to substitutions of the native SsoSSB sequence, SEQ ID NO:1, persons of skill will be aware that it is not necessary to first synthesize or express the protein and then to modify it. Typically, the practitioner decides on the substitution or substitutions desired, and assembles a nucleic acid vector that when expressed in a suitable host cell, such as E. coli, results in a protein with the desired sequence. Kits for engineering plasmids containing desired nucleic acid inserts and expressing such vectors in host cells are commercially available from a number of venders, and are well known in the art.
 The terms "identical" or percent "identity," in the context of two or more polypeptide sequences, refer to two or more sequences or subsequences that are the same or have a specified percentage of amino acid residues that are the same, when compared and aligned for maximum correspondence over a comparison window, as measured using one of the following sequence comparison algorithms or by manual alignment and visual inspection. When percentage of sequence identity is used in reference to proteins or peptides, it is recognized that residue positions that are not identical often differ by conservative amino acid substitutions, where amino acids residues are substituted for other amino acid residues with similar chemical properties (e.g., charge or hydrophobicity) and therefore do not change the functional properties of the molecule. Where sequences differ in conservative substitutions, the percent sequence identity may be adjusted upwards to correct for the conservative nature of the substitution. Means for making this adjustment are well known to those of skill in the art. Typically this involves scoring a conservative substitution as a partial rather than a full mismatch, thereby increasing the percentage sequence identity. Thus, for example, where an identical amino acid is given a score of 1 and a non-conservative substitution is given a score of zero, a conservative substitution is given a score between zero and 1. The scoring of conservative substitutions is calculated according to, e.g., the algorithm of Meyers & Miller, Computer Applic. Biol. Sci. 4:11-17 (1988), e.g., as implemented in the program PC/GENE (Intelligenetics, Mountain View, Calif., USA).
 As noted, one type of substitution is termed a "conservative substitution." One of skill will recognize that individual substitutions, in a peptide, polypeptide, or protein sequence which alters a single amino acid or a small percentage of amino acids in the encoded sequence are "conservatively modified variants" where the alteration results in the substitution of an amino acid with a chemically similar amino acid. Conservative substitution tables providing functionally similar amino acids are well known in the art. The following six groups each contain amino acids that are generally considered to be conservative substitutions for one another:  1) Alanine (A), Serine (S), Threonine (T);  2) Aspartic acid (D), Glutamic acid (E);  3) Asparagine (N), Glutamine (Q);  4) Arginine (R), Lysine (K);  5) Isoleucine (I), Leucine (L), Methionine (M), Valine (V); and  6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W).  (see, e.g., Creighton, Proteins (1984)). The phrase "substantially identical," in the context of two polypeptides, refers to sequences or subsequences that have at least 60%, preferably 70%, more preferably 80%, most preferably 90-95% amino acid residue identity when aligned for maximum correspondence over a comparison window as measured using one of the following sequence comparison algorithms or by manual alignment and visual inspection. This definition also refers to the complement of a test sequence, which has substantial sequence or subsequence complementarity when the test sequence has substantial identity to a reference sequence.
 One of skill in the art will recognize that two polypeptides can also be "substantially identical" if the two polypeptides are immunologically similar. Thus, overall protein structure may be similar while the primary structure of the two polypeptides display significant variation. Therefore, a method to measure whether two polypeptides are substantially identical involves measuring the binding of monoclonal or polyclonal antibodies to each polypeptide. Two polypeptides are substantially identical if the antibodies specific for a first polypeptide bind to a second polypeptide with an affinity of at least one third of the affinity for the first polypeptide.
 For sequence comparison, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are input into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. The sequence comparison algorithm then calculates the percent sequence identity for the test sequence(s) relative to the reference sequence, based on the designated program parameters.
 Optimal alignment of sequences for comparison can be conducted, e.g., by the local homology algorithm of Smith & Waterman, Adv. Appl. Math. 2:482 (1981), by the homology alignment algorithm of Needleman & Wunsch, J. Mol. Biol. 48:443 (1970), by the search for similarity method of Pearson & Lipman, Proc. Nat'l. Acad. Sci. USA 85:2444 (1988), by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, Wis.), or by visual inspection (see generally, Current Protocols in Molecular Biology, F. M. Ausubel et al., eds., Current Protocols, a joint venture between Greene Publishing Associates, Inc. and John Wiley & Sons, Inc., (1995 Supplement) (Ausubel)).
 Examples of algorithms that are suitable for determining percent sequence identity and sequence similarity are the BLAST and BLAST 2.0 algorithms, which are described in Altschul et al. (1990) J. Mol. Biol. 215: 403-410 and Altschul et al. (1997) Nucleic Acids Res. 25: 3389-3402, respectively. Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov/). This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold (Altschul et al, supra). These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are then extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always >0) and N (penalty score for mismatching residues; always <0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLASTN program (for nucleotide sequences) uses as defaults a wordlength (W) of 11, an expectation (E) of 10, M=5, N=-4, and a comparison of both strands. For amino acid sequences, the BLASTP program uses as defaults a wordlength (W) of 3, an expectation (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff & Henikoff, Proc. Natl. Acad. Sci. USA 89:10915 (1989)).
 In addition to calculating percent sequence identity, the BLAST algorithm also performs a statistical analysis of the similarity between two sequences (see, e.g., Karlin & Altschul, Proc. Nat'l. Acad. Sci. USA 90:5873-5787 (1993)). One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two amino acid sequences would occur by chance.
 "Conservatively modified variants" applies to both amino acid and nucleic acid sequences. With respect to particular nucleic acid sequences, conservatively modified variants refers to those nucleic acids which encode identical or essentially identical amino acid sequences, or where the nucleic acid does not encode an amino acid sequence, to essentially identical sequences. Because of the degeneracy of the genetic code, a large number of functionally identical nucleic acids encode any given protein. For instance, the codons GCA, GCC, GCG and GCU all encode the amino acid alanine. Thus, at every position where an alanine is specified by a codon, the codon can be altered to any of the corresponding codons described without altering the encoded polypeptide. Such nucleic acid variations are "silent variations," which are one species of conservatively modified variations. One of skill will recognize that each codon in a nucleic acid (except AUG, which is ordinarily the only codon for methionine) can be modified to yield a functionally identical molecule. Accordingly, each silent variation of a nucleic acid which encodes a polypeptide is implicit in any particular described sequence.
 The following examples are offered to illustrate, but not to limit the claimed invention.
 This Example sets forth materials and methods used in the studies reported herein.
 Alignment of protein sequences. The S. solfataricus ssb sequence (SSO2364) encoding the SSB protein (SsoSSB) was identified using BLASTP at the S. solfataricus genome website: http://www-archbac.u-psud.fr/projects/sulfolobus. The A. pernix ssb sequence (gi5105001) encoding the SSB protein (ApeSSB) was identified using BLAST at the PEDANT website: http://pedant.gsf.de/. Both open reading frames were recognized through their homology to MJ1159, encoding the Methanococcus jannaschii RPA protein (Chedin, F. et al., Trends Biochem Sci 23:273-277 (1998)). Subsequent alignments were performed using the ALIGN program at http://www.toulouse.inra.fr/multalin.html and additional features were highlighted by manual adjustment. BestFit comparisons were performed using the Wisconsin Package Version 10.1, Genetics Computer Group (GCG), Madison, Wis. and were between S. cerevisiae RPA70 single-stranded DNA-binding region 1 (gi6319321, amino acids 301-399) and the following single-stranded DNA-binding protein sequences; Archaeoglobus fulgidus (gi11497994), A. pernix (gi5105001), Bacillus subtilis (gi2127217), Escherichia coli (gi134913) Homo sapiens (gi1350579), M. jannaschii (MJ1159), Methanobacterium thermoautotrophicum (gi2622495), Pyrococcus abyssii (gi5457718), Pyrococcus horikoshii (gi3258332), S. solfataricus (SSO2364), as well as between E. coli SSB protein (gi134913) and S. solfataricus (SSO2364). The A. fulgidus sequence used was one of two identified as homologous to MJ1159 and is the most homologous to the N-terminus of the M. jannaschii protein (Chedin 1998). The M. thermoautotrophicum sequence was adjusted to account for the frameshift identified by Chedin 1998.
 Strains and cultivation. S. solfataricus strain P2 (DSM 1616, (Zillig, W. et al., Arch Microbiol 125:259-269 (1980)) was the generous gift of Dennis Grogan (University of Cincinnati) and was grown at 80° C. as described (Rolfsmeier, M. et al., J Bacteriol 180:1287-1295 (1998)) at a pH of 3.0 in screw cap flasks as described (Rolfsmeier, M. et al., J Bacteriol 177:482-485 (1995)). Basal salts medium was Allen's medium (Allen M. B., Arch. Mikrobiol., 32:270-277 (1959)) as modified by Brock (Brock, T. D. et al., Arch Mikrobiol 84:54-68 (1972)) and was supplemented with tryptone to a final concentration of 0.2% (w/v). Growth was monitored spectrophotometrically at a wavelength of 540 nm. Escherichia coli strains were DH5α (φ80dlacZAΔ15, endA1, recA1, hsdR17 (rk-,mk.sup.+), supE44, thi-1, gyrA96, relA1, Δ(lacZYA-argF)U169); BL21(DE3) (ompT [lon] hsdSB (rB-mB-; an E. coli B strain) with DE3, a λ prophage carrying the T7 RNA polymerase gene); and KLC789 (F, metA7, rha8, thyA36, amp50, deoC2, ssb-1) (Chase, J. W. et al., J Mol Biol 164:193-211 (1983)) from laboratory collections or BL21(DE3) CodonPlus ultracompetent cells from Stratagene. E. coli was propagated in LB medium (Sambrook, J. et al., Molecular cloning: a laboratory manual. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press (1989)) at 30° C. in Ehrlenmeyer flasks shaken at 250 rpm.
 PCR amplification and cloning of the SsoSSB gene. Genomic DNA was prepared from S. solfataricus cells as previously described (Rolfsmeier, M. et al., J Bacteriol 180:1287-1295 (1998)). PCR was performed using 10 mM potassium chloride, 10 mM ammonium sulfate, 2 mM magnesium chloride, 20 mM Tris-Cl (pH 8.75), 0.1% Triton X-100, 100 μM dNTP's, 100 pmol primers, 2 ng template DNA, and 2.5 U of recombinant Pfu DNA polymerase (Stratagene). The primers for amplification of SsoSSB were: 5'-CGGGATCCCCTTTCA TTAACACATAGATTTATAAATGG-3' (SEQ ID NO:8) (SSB-F) and 5'-CGGGATCCGGAGCAA GCTCGTATACTTTGTCTCTAGCC-3' (SEQ ID NO:9) (SSB-R). All primer sequences were chosen based on sequence information presented at the S. solfataricus genome website: http://www-archbac.u-psud.fr/projects/sulfolobus. PCR was performed using a 55° C. annealing temperature and the resulting PCR products were digested with BamHI and ligated into the BamHI site of pUC19. Ligated molecules were transformed into DH5α as previously described (Rolfsmeier, M. et al., J Bacteriol 180:1287-1295 (1998)). Plasmids from transformants were isolated using the Qiagen midiprep system and DNA sequences were determined using BigDye dRhodamine Terminator chemistry (Perkin-Elmer Corp.) at the Division of Biological Sciences Automated DNA sequencing facility at UC Davis.
 Overexpression of SsoSSB protein. Sequence information obtained from the pUC19 clones was used to design a forward PCR primer with an NdeI site at the starting ATG codon for SsoSSB. The gene sequence was re-amplified from the cloned template using the new forward primer (5'-GTGAGTCGAGTCATATGGAAG-3') (SEQ ID NO:10) and the original reverse primer. The resulting product was digested using NdeI and BamHI prior to ligation into pET21a (Novagen) that had been digested with the same enzymes to place the gene under the control of the T7 promoter. Ligation products were transformed into the CodonPlus strain (Stratagene) and transformants were cultivated at 30° C. in LB containing 100 μg/ml ampicillin until mid-log phase.
 Purification of SsoSSB protein. BL21(DE3) CodonPlus cells (Stratagene) harboring the pET21a SsoSSB expression construct were grown at 30° C. in a 500 ml volume to an OD600 of 1.0. IPTG was added to a final concentration of 1 mM and expression was allowed to continue for 2 hours. Cells were harvested by centrifugation and stored at -20° C. until processing. The frozen cell pellet was resuspended in 4 ml of 10 mM Tris-Cl (pH 7.5), 1 mM EDTA (TE) with 50 mM NaCl and sonicated to disrupt the cells. The sonicate was heat treated at 80° C. for 1 hour, and insoluble material was removed by centrifugation. Clarified sonicate was applied to a ssDNA cellulose column equilibrated in 30 mM Tris-Cl (pH 7.5), 1 mM EDTA, 1 mM DTT, and 10% glycerol; the column was washed with the same buffer containing 0.5 M NaCl and 0.75 M NaCl at room temperature. Fractions eluting at 0.75 M NaCl were pooled and dialyzed into buffer containing 20 mM Tris-Cl (pH 7.5), 1 mM DTT, 1 mM EDTA, and 10% glycerol. This material was then applied to a Resource Q column (Pharmacia) equilibrated with the same buffer at room temperature. Protein was eluted using a gradient of 50 mM NaCl to 1 M NaCl in the same buffer; the SsoSSB protein eluted from the column at approximately 60 mM NaCl. The protein was pooled and concentrated by using dry polyethylene glycol and then dialyzed against 25 mM Tris HCl (pH 7.5), 20 mM NaCl, 1 mM EDTA, 1 mM DTT, 10% spectral grade glycerol, and stored at 4° C. Protein concentrations were obtained by spectrophotometric absorbance at a wavelength of 280 nm, using an extinction coefficient of 12660 M-1 cm-1 as determined with the ProtParam tool at the ExPASy website (http://expasy.cbr.nrc.ca/tools/protparam.html).
 Gel filtration of SsoSSB protein. Fast protein liquid chromatography (FPLC) was performed at 4° C. using a Superose 12 column (Pharmacia) and 25 mM Tris HCl pH 7.5, 1 mM DTT, 100 mM NaCl, 1 mM EDTA as the running buffer. Molecular size standards were BSA (66 kDa), carbonic anhydrase (29 kDa), and cytochrome C (12.4 kDa) and were prepared in running buffer. A total of 10 μg of SsoSSB protein was loaded on the column in a volume of 100 μl. Elution profiles were determined by monitoring OD280 readings and a standard curve was prepared by plotting Ve/Vo against the molecular mass of the size standards. The value of Vo was determined by elution of dextran blue from the Superose 12 column.
 Gel mobility-shift analysis. The 63-mer oligonucleotide 5'-ACAGCACCAAT GAAATCTATTAAGCTCCTCATCGTCCGCAAAAATATCGTCACCTCAAAAGGA-3' (SEQ ID NO:11) was end-labeled with 32P using T4 polynucleotide kinase (NEB). SsoSSB protein was incubated at the indicated concentrations with 10 μM (nucleotides) of the 32P-labeled oligonucleotide for 30 minutes at 75° C. in buffer containing 30 mM TrisOAc (pH 7.5), 10 mM MgOAc2, 5 mM NaCl, 0.1 mM DTT and 50 μg/ml BSA. Increasing concentrations of linearized pUC19 were used as the dsDNA competitor as indicated in the text. Loading dye was then added and the samples were applied to a vertical 10% acrylamide gel prepared with 1×TBE buffer (0.089 M Tris-borate, 0.089 M boric acid, 0.002 M EDTA).
 In vivo complementation. The SsoSSB expression vector or pET21a (empty vector) was transformed into E. coli strain KLC789 (Chase, J. W. et al., J Mol Biol 164:193-211 (1983)) containing pTara, a T7 polymerase expression vector that is inducible by arabinose addition. The pTara plasmid was the generous gift of Kathleen Mathews (Rice University) (Wycuff, D. R. et al., Anal Biochem 277:67-73 (2000)). Transformants were propagated in LB medium lacking yeast extract with 0.2% (w/v) arabinose, 100 μg/ml ampicillin, and 30 μg/ml chloramphenicol for 16 hours at 30° C. to allow phenotypic overexpression of SsoSSB protein. Control cultures were propagated identically, except 0.2% (w/v) glucose was substituted for arabinose. Cells were subcultured into fresh medium without chloramphenicol and grown at 30° C. until they were shifted to the non-permissive temperature of 43° C. Optical densities were monitored spectrophotometrically at a wavelength of 600 nm. Colony forming units (cfu) per milliliter were determined by plating serial dilutions of each timepoint in triplicate on LB medium. Plates were incubated overnight at 30° C. prior to scoring for viable counts.
 DNA strand exchange reactions. E. coli RecA protein (11 μM) was incubated with φX174 ssDNA (New England Biolabs) at a concentration of 33 μM (nucleotides) in 30 mM TrisOAc (pH 7.5), 10 mM DTT, 20 mM MgOAc, 2.5 mM ATP, and 5 μg/ml BSA at 37° C. for 10 minutes. After the addition of either 2.2 μM SsoSSB protein or E. coli SSB protein, reaction mixtures were incubated at 37° C. for another 5 min before the introduction of PstI-linearized φX174 dsDNA (New England Biolabs) at a concentration of 33 μM (nucleotides). The reaction mixtures were then incubated at 37° C. for 90 minutes and were stopped by the addition of SDS to a final concentration of 0.6% and proteinase K to a final concentration of 1 μg/ml. Deproteinization of the reaction mixtures was carried out at 37° C. for 10 minutes.
 Gel electrophoresis. Agarose gels for evaluation of cloning procedures were prepared at 0.8% and run at approximately 150 V in TBE buffer prior to staining with ethidium bromide. Tricine SDS/PAGE, used to monitor protein purification, was prepared with a 4% stacking gel and a 20% separating gel as described (Price, L. B. et al., J Bacteriol 182:4951-4958 (2000)) and run at 100 V. Acrylamide gels for gel mobility-shift analysis were prepared at 10% in TBE buffer and run at 100 V for 3 hours at 65° C. prior to exposure to phosphorimaging screens and analysis with a Storm 840 Phosphorlmager (Molecular Dynamics). Agarose gels for evaluation of DNA strand exchange products were prepared at 1% and run at approximately 30 V in TAE buffer (0.04 M TrisOAc, 0.002 M EDTA) for 15 hours prior to staining with ethidium bromide.
 This Example sets forth the results of studies conducted using the methods and materials set forth in the previous Example.
Crenarchaeal Homologues of ssDNA Binding Proteins.
 Previous searches of genome sequences identified open reading frames with homology to human RPA70 from the euryarchaeons M. jannachii, M. thermoautrophicum, and A. fulgidus (Chedin, F. et al., Trends Biochem Sci 23:273-277 (1998); Kelly, T. J. et al., Proc Natl Acad Sci USA 95:14634-14639 (1998)). Each of these sequences consists of multiple ssDNA-binding domains contained in one or a pair of open reading frames, which share a significant degree of sequence homology among themselves. In one case, the protein encoded by this homologous sequence was shown to be an RPA homologue both structurally and functionally (Kelly, T. J. et al., Proc Natl Acad Sci USA 95:14634-14639 (1998)). However, there has been no corresponding homologue of RPA identified in members of the other branch of the archaeal domain, the crenarchaea. The availability of sequence information from both A. pernix (Kawarabayasi, Y. et al., DNA Res 6:83-101:145-152 (1999)) and S. solfataricus (She, Q. et al., Proc Natl Acad Sci USA 98:7835-7840 (2001)) permitted a search for ssDNA-binding protein sequences in the crenarchaea.
 A survey of genome sequences from A. pernix and S. solfataricus with the program BLAST (Altschul, S. F. et al., J Mol Biol, 215:403-410, (1990)) revealed a single small open reading frame in each genome with sequence similarity to MJ1159, the RPA homologue from M. jannaschii. Both open reading frames code for proteins that are strikingly similar to the first ssDNA-binding domain of the M. jannaschii RPA (FIG. 1a), but are much shorter than the M. jannaschii protein. The S. solfataricus sequence (SSO2364) is 477 base pairs in length and encodes a protein of 148 amino acids while the A. pernix sequence (gi5105001) is 429 base pairs in length and encodes a protein of 143 amino acids; in contrast, the M. jannaschii protein is 645 amino acids in length. The S. solfataricus protein has a predicted pI of 9.0, and the A. pernix protein also has a predicted pI of 9.0. For comparison, the pI's of the T4 phage SSB homologue gp32, E. coli SSB, and M. jannaschii RPA are 4.8, 5.4, and 4.7 respectively. The S. solfataricus protein has 52% similarity and 26% identity with MJ1159 from amino acids 68 to 170 and the A. pernix protein has 52% similarity and 25% identity with MJ1159 from amino acid 70 to 173. The two residues conserved in all archaeal sequences correspond to amino acids involved in DNA binding in human RPA70 protein domain B (Thr 359 and Trp 361 in the hsRPA70 sequence) (Bochkarev, A. et al., Nature, 385:176-181 (1997)) (FIG. 1a). Additionally, the S. solfataricus sequence shares two other amino acids with human RPA70 that are implicated in DNA binding, Phe 386 and Ser 396 (Bochkarev, A. et al., Nature, 385:176-181 (1997)).
 The two crenarchaeal protein sequences show a remarkable level of homology between each other, exhibiting 69% similarity and 47% identity (FIG. 1). Both of the newly identified open reading frames are significantly shorter than their euryarchaeal and eukaryotic counterparts, each comprising only a single ssDNA-binding region as opposed to closely related tandem repeats (four, in the case of the M. jannaschii protein) of multiple binding regions in a larger polypeptide. Examination of nearby regions in each genome revealed no further sequences that might encode for portions of a ssDNA-binding protein, suggesting either that S. solfataricus SSO2364 and A. pernix gi5105001 are the only genes responsible for producing SSB for these crenarchaeons or that there were other genes elsewhere in the genome that encoded the remainder of the subunits for an RPA-like protein. No other sequence with significant similarity to MJ1159 is apparent elsewhere in the complete genomes of either A. pernix or S. solfataricus, suggesting that these proteins may serve as the sole SSBs in these organisms.
The Crenarchaeal Proteins Structurally Resemble E. coli SSB Protein.
 In contrast to other archaeal SSB homologues which resemble eukaryotic RPAs, it appears that the overall architecture of the crenarchaeal versions is distinctly different. Euryarchaeal RPA homologues are comprised of multiple DNA-binding domains within one or a pair of proteins and presumably function in monomeric or heterodimeric forms, respectively (FIG. 2). The open reading frames from both A. pernix and S. solfataricus, however, are distinguished by a single core ssDNA-binding domain of about 100 amino acids in length, identical to that observed in eubacterial ssDNA-binding proteins. This suggests that the quaternary structure of the crenarchaeal SSB protein might be analogous to the eubacterial version and could be tetrameric. Additionally, the crenarchaeal proteins lack the C-terminal zinc finger of the euryarchaeal RPAs and instead, contain a number of acidic residues in this region. This is analogous to the C-terminal structure of E. coli SSB, which is required for protein-protein interactions (Chase, J. W. et al., J Biol Chem 260:7214-7218 (1985); Kelman, Z. et al., EMBO J 17:2436-2449 (1998); Williams, K. R. et al., J Biol Chem 258:3346-3355 (1983)). Also absent in the crenarchaeal sequences is an approximately 100 residue N-terminal region found in euryarchaeal RPAs that is believed to be involved in protein binding (Wold, M. S. Annu Rev Biochem 66:61-92 (1997)).
 The SSB protein homologue of phage T4, gp32, has little sequence homology to bacterial SSBs but displays both structural similarity with these proteins and an acidic C-terminus (Shamoo, Y. et al., Nature 376:362-366 (1995)). Much like gp32, however, the crenarchaeal SSBs share minimal sequence homology with E. coli SSB. BestFit alignment of the crenarchaeal sequences with E. coli SSB shows 35% similarity and 31% identity over only 48 amino acids for SsoSSB and 43% similarity and 38% identity over only 60 amino acids for ApeSSB. However, the crenarchaeal proteins do appear to share structural similarity with the eubacterial protein. Despite reduced sequence homology with E. coli SSB, homology-dependent modeling of the expected structure for S. solfataricus SSB reveals striking similarity between the two proteins in the core DNA binding region; a similar analysis was used to confirm the previous identification of the euryarchaeal RPA homologues. The modeling shows strong evolutionary structural conservation of the OB-fold (residues 32-71) which is implicated in DNA binding, as well as the α-helix, which is involved in subunit interactions (Webster et al, FEBS Lett, 411:313-316 (1997)). Equivalent modeling using hsRPA70 also demonstrated strong structural conservation, especially with the RPA-B subdomain. These results confirm that the open reading frame from S. solfataricus encodes a ssDNA-binding protein.
Expression and Purification of the SSB Homologue from S. solfataricus.
 To determine if SSO2364 from S. solfataricus did indeed encode a functional SSB protein homologue, we purified and characterized the candidate protein. The SSO2364 open reading frame was cloned into the pET21a bacterial expression vector and subsequently heterologously expressed in E. coli. The bacterial strain carried a plasmid encoding three rare tRNA genes in an effort to reduce translational pausing as the predicted protein sequence contains 11 codons that are rare in E. coli, four of which are present in the final 50 bases of the open reading frame. The expressed protein is soluble and was purified to near homogeneity by first heat-treating the cell sonicate at 80° C. to denature all mesophilic proteins. This step was followed by affinity chromatography on ssDNA-cellulose, and by anion exchange chromatography on Resource Q. The recombinant protein eluted from ssDNA-cellulose at 0.75 M NaCl, which is identical to the salt concentration necessary for elution of E. coli SSB (Lohman, T. M. et al., Biochemistry 25:21-25 (1986)). In contrast, elution of either yeast or M. jannachii RPA from the same matrix requires 1.5 M NaCl with the addition of 40% ethylene glycol, 1.3 M potassium thiocyanate, or 1.5 M sodium thiocyanate (Henricksen, L. A. et al., J Biol Chem 269:24203-24208 (1994); Heyer, W. D. et al., Embo J 9:2321-2329 (1990); Kelly, T. J. et al., Proc Natl Acad Sci USA 95:14634-14639 (1998)), suggesting that the S. solfataricus SSB (SsoSSB) protein is chemically more similar to the bacterial protein than it is to RPA. The SsoSSB protein eluted at approximately 60 mM NaCl from Resource Q and pooled fractions yielded essentially pure protein (greater than 98% purity based on Coomassie stained gels). Examination of the purified protein by SDS/PAGE revealed a single band with a molecular weight of approximately 16 kDa, in close agreement with the predicted molecular weight of 16,138 D as determined from the amino acid sequence (FIG. 3).
The SsoSSB Protein Forms Tetramers in Solution.
 It was of interest to determine if the purified S. solfataricus protein would multimerise in solution. Analytical gel filtration was used to determine the native form of the recombinant protein. Examination of the SsoSSB protein in solution revealed that it was present in three distinct species: monomeric, dimeric, and tetrameric forms, at 18 kDa, 36 kDa, and 62 kDa, respectively (FIG. 4a). As demonstrated by a representative elution profile (FIG. 4b), the composition of the purified material was primarily tetrameric, while somewhat less dimeric protein was present. A significantly smaller quantity of the monomeric form of SsoSSB protein was observed. This result indicates that the SsoSSB protein indeed forms tetramers in solution. The M. jannachii RPA protein does not multimerize in solution (Kelly, T. J. et al., Proc Natl Acad Sci USA 95:14634-14639 (1998))).
SsoSSB Protein Binds ssDNA.
 To determine the occluded binding site size of the protein and to evaluate the activity of the SsoSSB protein, we performed acrylamide gel mobility-shift assays with a radioactively labeled single-stranded oligonucleotide that was 63 nucleotides in length (FIG. 5). When a fixed quantity of radiolabeled oligonucleotide was incubated with increasing quantities of protein, a band of reduced mobility was observed. The apparent mobility further decreased upon addition of more protein, finally achieving a constant mobility after the addition of 2 μM protein. The absence of discrete species implied rapid equilibration between bound and free forms of the complexes, and consistent with this possibility, more discrete species were visualized when electrophoresis was performed more rapidly (at high voltage). The addition of linear pUC19 DNA to a 100-fold molar (nucleotide) did not alter the mobility-shift pattern, demonstrating that the SsoSSB has an, at least, 100-fold greater affinity for ssDNA than for dsDNA. Saturation was achieved at a ratio of approximately one SSB protein molecule to 5 nucleotides. The M. jannaschii RPA protein has a site size of 15 to 20 nucleotides (Kelly, T. J. et al., Proc Natl Acad Sci USA 95:14634-14639 (1998)) whereas the site size of E. coli SSB protein, depending on solution conditions, varies from 8 to 16 nucleotides per monomer. The apparent site size of SsoSSB protein is similar to the 7 nucleotide site size observed for phage T4 SSB protein, gp32. Binding of SsoSSB protein to ssDNA does not appear to be a cooperative process under these conditions, as intermediately shifted species are evident instead of the approximately 2-state transitions that typify cooperative binding. Rather, a steady reduction in the mobility of protein-DNA complexes occurs as protein concentration is increased, suggesting that binding of SsoSSB protein to ssDNA is distributive in nature. The observed band-retardation pattern is likely the result of a combination of this non-cooperative binding and rapid equilibration of protein-DNA complexes during electrophoresis.
SsoSSB Protein is a Functional SSB Homologue.
 In E. coli, SSB is an essential protein. A number of mutations were used to elucidate the function of E. coli SSB protein; one mutation is the temperature sensitive mutation called ssb-1. The ssb-1 mutation is an alteration of amino acid 55 from a histidine to a tyrosine that is believed to destabilize the tetrameric SSB protein complex upon shifting temperature from 30° C. to the non-permissive temperature of 43° C., resulting in a lethal phenotype (Chase, J. W. et al., J Mol Biol 164:193-211 (1983)). Overexpression of wild-type E. coli SSB protein encoded on a plasmid can overcome the lethality of the mutation (Chase, J. W. et al., J Mol Biol 164:193-211 (1983)) as can the SSB protein of bacteriophage P1 (Lehnherr, H. et al., J Bacteriol 181:6463-6468 (1999)). To test the in vivo functionality of SsoSSB, the protein was overexpressed in the E. coli strain KLC789, which carries the ssb-1 allele. The SsoSSB plasmid was transformed into KLC789 along with a plasmid carrying an arabinose-inducible T7 polymerase gene. Cells were grown in the presence of arabinose for 16 hours at the permissive temperature to allow over-expression of the SsoSSB protein, while control cultures were cultivated in the presence of glucose. Cells were then shifted to the non-permissive temperature and monitored for further growth (FIG. 6a). The presence of the open reading frame encoding SsoSSB protein in pET21a permitted continued growth of the ssb-1 mutant strain while the pET21a vector alone was unable to rescue the lethal phenotype. Rescued growth required the presence of arabinose, as control experiments with glucose showed no increase in optical density that would be consistent with continued growth. To verify that the cells containing the SsoSSB plasmid were indeed still growing, viable counts for each timepoint were determined by plating (FIG. 6b). Following the temperature shift, the control cultures continued to grow for a brief period and then dramatically lost viability. In contrast, the cells containing the SsoSSB plasmid displayed a continuous increase in viable counts. These results indicate that SsoSSB protein is capable of replacing E. coli SSB protein in vivo and that it functions at 43° C.
 SsoSSB Protein Stimulated DNA Strand Exchange by E. coli RecA Protein.
 It was previously shown that heterologously expressed SSB proteins can stimulate DNA strand exchange mediated by E. coli RecA protein (Egner, C. et al., J Bacteriol 169:3422-3428 (1987)). To demonstrate the functionality of the SsoSSB protein in at least one nucleic acid metabolic function in vitro, DNA strand exchange reactions were performed (FIG. 7). Purified SsoSSB protein was capable of substituting for E. coli SSB protein in DNA strand exchange reactions mediated by E. coli RecA protein at 37° C. using homologous φX174 ss- and dsDNA. Clear enhancement of DNA strand exchange, as determined by production of nicked circular product, was observed with SsoSSB protein (FIG. 7, lane 3). Interestingly, the M. jannaschii RPA protein can also promote RecA-mediated DNA-strand exchange. The reduced amount of nicked circular product in reactions containing SsoSSB protein relative to levels with E. coli SSB protein may be the result of performing the experiment at temperatures where SsoSSB protein is not as effective. The optimal temperature for the SsoSSB protein is expected to be near the growth temperature of S. solfataricus, which is 80° C. As yet, no enhancement of strand exchange activity through addition of SsoSSB protein to S. solfataricus RadA protein-containing reactions at high temperature has been observed, though this may be a consequence of inappropriate assay conditions or may signify a post-synaptic role for SsoSSB protein at physiological temperatures.
 This Example discusses the results of the studies set forth above.
 Divergence between the archaeal and eukaryotic lineages is more recent than the divergence of the bacterial and eukaryotic/archaeal groups (Olsen, G. J. et al., Cell 89:991-994 (1997)). Accordingly, a number of features shared by eukaryotes and archaea, but not bacteria, including replication and transcription proteins were likely obtained after evolutionary divergence of these three groups. In contrast, features found in archaea and bacteria but not in eukaryotes including morphological attributes (a lack of organelles and nucleus), coupling of transcription and translation, conjugative mechanisms, and a single circular genome may be reminiscent of a more ancient, shared ancestor.
 However, despite the logic of this viewpoint, the evolutionary behavior of the archaea is not so simplistic. Our results indicate that, in contrast to the canonical expectation that all archaeal ssDNA binding proteins would be RPA-like, the crenarchaeal SSBs share sequence homology with eukaryal RPAs but structural homology with bacterial SSBs. Homology-dependent modeling of SsoSSB demonstrates conservation of the OB-fold, indicative of ssDNA binding proteins. Both E. coli SSB and SsoSSB proteins elute from ssDNA-cellulose at the same salt concentration, and both form tetramers in solution. The apparent binding site size is approximately 5 nucleotides, which is consistent with the site size observed for T4 g32 protein and the low site size binding mode of E. coli SSB protein, but is one-fourth that of M. jannachii RPA protein, which has four DNA-binding domains. Another feature that makes the crenarchaeal SSB protein more eubacterial is that SsoSSB protein and ApeSSB protein are missing the zinc finger motif near the C-terminus which is present in both euryarchaeal and eukaryal RPAs, suggesting that this motif was acquired after the separation of the two archaeal branches. Furthermore, the acidic residues in the SsoSSB protein C-terminal region are similar to those found in bacterial proteins and may have been maintained from the last shared bacterial and archaeal ancestor. SsoSSB protein resembles E. coli SSB chemically in that they both elute from ssDNA-cellulose at 0.75 M NaCl while the euryarchaeal and eukaryal RPAs require higher salt concentrations and the addition of ethylene glycol, potassium thiocyanate, or sodium thiocyanate for elution from this matrix. Finally, SsoSSB protein can functionally substitute for E. coli SSB protein both in vivo and in vitro. In vivo, overexpression of the SsoSSB protein can overcome the lethal ssb-1 temperature-sensitive mutation in E. coli; in vitro, the SsoSSB protein can replace the E. coli SSB protein in DNA strand exchange reactions mediated by RecA protein.
 Concurrent with our efforts, the same crenarchaeal protein was identified by another laboratory using biochemical criteria (Wadsworth, R. I. et al., Nucleic Acids Res 29:914-920 (2001)). No multimerisation of the protein was demonstrated, contrary to our observation. In our experience, however, heating of the SsoSSB protein is necessary for formation of the tetrameric structure as observed by gel filtration and for activity in DNA strand-exchange. The absence of this heating step by Wadsworth and White could account for their failure to observe tetramers.
 Overall, the archaeal ssDNA binding proteins show more sequence similarity to eukaryotic RPA proteins than to E. coli SSB protein. However, the common sequences and DNA-binding domain utilization hint at a conserved evolutionary relationship between RPA and SSB (FIG. 2). Strong homology among the archaeal proteins suggests a common evolutionary origin, and the crenarchaeal SSB proteins may represent a link to ancient ssDNA-binding proteins. The crenarchaeal SSB protein, being one of the simplest in structure, may represent the earliest form of single-stranded DNA-binding proteins involved in recombination and, it may serve as a model for understanding structure, function, and evolution of the conserved core ssDNA-binding domain.
 Crenarchaea and euryarchaea are distinguished from each other not only by single-stranded DNA-binding proteins as described here, but also by double-stranded DNA-binding proteins. While eukaryotic-like histone proteins have been identified and extensively studied in members of the euryarchaea (Sandman, K. et al., Arch Microbiol 173:165-169 (2000)), no such homologues are apparent in members of the crenarchaea. Instead, crenarchaea maintain sequences that code for small, basic dsDNA-binding proteins (Agback, P. et al., Nat Struct Biol, 5:579-584 (1998)); Faguy, D. M. et al., Curr Biol 9:R883-886 (1999)). These proteins are proposed to be comparable to histone-like proteins in eubacteria, especially Sso7d which was shown to be analogous to HU (Lopez-Garcia, P. et al., Nucleic Acids Res 26:2322-2328 (1998). It appears that the crenarchaea have maintained both single-stranded and double-stranded DNA-binding proteins that are more similar to those found in bacteria than they are to eukaryotic homologues. Crenarchaeal dsDNA-binding proteins and SSB may have evolved separately from mechanisms employed by eukaryotes or, alternatively, crenarchaea diverged earlier, prior to the co-evolution of eukaryotic-like histones and RPA. The use of a eubacterial-like SSB may necessitate a eubacterial DNA-binding protein for the compaction of DNA. The discovery of an SSB homologue in the crenarchaea that is significantly more similar to eubacterial SSB both structurally and biochemically than it is to either euryarchaeal or eukaryotic RPA further establishes the evolutionary distance between the two archaeal phyla.
 It is understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application and scope of the appended claims. All publications, patents, and patent applications cited herein are hereby incorporated by reference in their entirety for all purposes.
121148PRTSulfolobus solfataricussingle-stranded (ssDNA) binding protein (SSB) SSO2364 (SsoSSB) 1Met Glu Glu Lys Val Gly Asn Leu Lys Pro Asn Met Glu Ser Val Asn1 5 10 15Val Thr Val Arg Val Leu Glu Ala Ser Glu Ala Arg Gln Ile Gln Thr 20 25 30Lys Asn Gly Val Arg Thr Ile Ser Glu Ala Ile Val Gly Asp Glu Thr 35 40 45Gly Arg Val Lys Leu Thr Leu Trp Gly Lys His Ala Gly Ser Ile Lys 50 55 60Glu Gly Gln Val Val Lys Ile Glu Asn Ala Trp Thr Thr Ala Phe Lys65 70 75 80Gly Gln Val Gln Leu Asn Ala Gly Ser Lys Thr Lys Ile Ala Glu Ala 85 90 95Ser Glu Asp Gly Phe Pro Glu Ser Ser Gln Ile Pro Glu Asn Thr Pro 100 105 110Thr Ala Pro Gln Gln Met Arg Gly Gly Gly Arg Gly Phe Arg Gly Gly 115 120 125Gly Arg Arg Tyr Gly Arg Arg Gly Gly Arg Arg Gln Glu Asn Glu Glu 130 135 140Gly Glu Glu Glu1452447DNASulfolobus solfataricussingle-stranded (ssDNA) binding protein (SSB) SSO2364 (SsoSSB) 2atggaagaaa aagtaggtaa tctaaaacca aatatggaaa gcgtaaatgt aaccgtaaga 60gttttggaag caagcgaagc aagacaaata cagacaaaga acggtgttag aacaatcagt 120gaggctattg ttggagatga aacgggaaga gtaaagttaa cattatgggg aaaacatgca 180ggtagtataa aagaaggtca agtggtaaag atagaaaacg cgtggaccac cgcttttaag 240ggtcaagtac agttaaatgc tggaagcaaa actaagatag ctgaagcttc agaagatgga 300tttccagaat catctcaaat accagaaaat acaccaacag ctcctcagca aatgcgtgga 360ggaggaagag gattccgcgg tgggggaaga aggtatggaa gaagaggtgg tagaagacaa 420gaaaacgaag aaggtgaaga ggagtga 447393PRTSulfolobus solfataricussingle-stranded (ssDNA) binding protein (SSB) SSO2364 (SsoSSB) ssDNA-binding domain 3Met Glu Glu Lys Val Gly Asn Leu Lys Pro Asn Met Glu Ser Val Asn1 5 10 15Val Thr Val Arg Val Leu Glu Ala Ser Glu Ala Arg Gln Ile Gln Thr 20 25 30Lys Asn Gly Val Arg Thr Ile Ser Glu Ala Ile Val Gly Asp Glu Thr 35 40 45Gly Arg Val Lys Leu Thr Leu Trp Gly Lys His Ala Gly Ser Ile Lys 50 55 60Glu Gly Gln Val Val Lys Ile Glu Asn Ala Trp Thr Thr Ala Phe Lys65 70 75 80Gly Gln Val Gln Leu Asn Ala Gly Ser Lys Thr Lys Ile 85 90488PRTAeropyrum pernixsingle-stranded (ssDNA) binding protein (SSB) (ApeSSB) ssDNA-binding domain 4Met Asp Leu Arg Glu Gly Leu Arg Asn Val Ser Ile Ser Gly Arg Val1 5 10 15Leu Glu Thr Gly Glu Pro Lys Met Val Glu Thr Lys Arg Gly Pro Ala 20 25 30Thr Leu Ser Glu Ala Val Val Gly Asp Glu Ser Gly Arg Val Lys Val 35 40 45Thr Leu Trp Gly Ser His Ala Gly Thr Leu Lys Glu Gly Glu Ala Val 50 55 60Arg Ile Glu Gly Ala Trp Thr Thr Ser Tyr Arg Gly Lys Val Gln Val65 70 75 80Asn Val Gly Arg Glu Ser Thr Ile 85591PRTMethanococcus jannaschiiMJ1159, replication protein A (RPA) ssDNA- binding domain 5Ile Ser Asp Ile Glu Glu Gly Gln Ile Gly Val Glu Ile Thr Gly Val1 5 10 15Ile Thr Asp Ile Ser Glu Ile Lys Thr Phe Lys Arg Arg Asp Gly Ser 20 25 30Leu Gly Lys Tyr Lys Arg Ile Thr Ile Ala Asp Lys Ser Gly Thr Ile 35 40 45Arg Met Thr Leu Trp Asp Asp Leu Ala Glu Leu Asp Val Lys Val Gly 50 55 60Asp Val Ile Lys Ile Glu Arg Ala Arg Ala Arg Lys Trp Arg Asn Asn65 70 75 80Leu Glu Leu Ser Ser Thr Ser Glu Thr Lys Ile 85 90699PRTSaccharomyces cerevisiaereplication protein A (RPA70) ssDNA-binding domain 6Asn Phe Ile Lys Leu Asp Ala Ile Gln Asn Gln Glu Val Asn Ser Asn1 5 10 15Val Asp Val Leu Gly Ile Ile Gln Thr Ile Asn Pro His Phe Glu Leu 20 25 30Thr Ser Arg Ala Gly Lys Lys Phe Asp Arg Arg Asp Ile Thr Ile Val 35 40 45Asp Asp Ser Gly Phe Ser Ile Ser Val Gly Leu Trp Asn Gln Gln Ala 50 55 60Leu Asp Phe Asn Leu Pro Glu Gly Ser Val Ala Ala Ile Lys Gly Val65 70 75 80Arg Val Thr Asp Phe Gly Gly Lys Ser Leu Ser Met Gly Phe Ser Ser 85 90 95Thr Leu Ile7100PRTHomo sapiensreplication protein A 70 kDa DNA-binding subunit (RPA70) ssDNA-binding domain 7Asp Phe Thr Gly Ile Asp Asp Leu Glu Asn Lys Ser Lys Asp Ser Leu1 5 10 15Val Asp Ile Ile Gly Ile Cys Lys Ser Tyr Glu Asp Ala Thr Lys Ile 20 25 30Thr Val Arg Ser Asn Asn Arg Glu Val Ala Lys Arg Asn Ile Tyr Leu 35 40 45Met Asp Thr Ser Gly Lys Val Val Thr Ala Thr Leu Trp Gly Glu Asp 50 55 60Ala Asp Lys Phe Asp Gly Ser Arg Gln Pro Val Leu Ala Ile Lys Gly65 70 75 80Ala Arg Val Ser Asp Phe Gly Gly Arg Ser Leu Ser Val Leu Ser Ser 85 90 95Ser Thr Ile Ile 100838DNAArtificial SequenceDescription of Artificial SequencePCR amplification forward primer SSB-F 8cgggatcccc tttcattaac acatagattt ataaatgg 38938DNAArtificial SequenceDescription of Artificial SequencePCR amplification reverse primer SSB-R 9cgggatccgg agcaagctcg tatactttgt ctctagcc 381021DNAArtificial SequenceDescription of Artificial Sequencenew PCR forward primer for reamplification from cloned template 10gtgagtcgag tcatatggaa g 211163DNAArtificial SequenceDescription of Artificial Sequence63-mer oligonucleotide end-labeled with 32-P 11acagcaccaa tgaaatctat taagctcctc atcgtccgca aaaatatcgt cacctcaaaa 60gga 63124PRTArtificial SequenceDescription of Artificial Sequenceconsensus peptide 12Thr Leu Trp Gly1
Patent applications by Stephen C. Kowalczykowski, Davis, CA US
Patent applications by REGENTS OF THE UNIVERSITY OF CALIFORNIA
Patent applications in class With significant amplification step (e.g., polymerase chain reaction (PCR), etc.)
Patent applications in all subclasses With significant amplification step (e.g., polymerase chain reaction (PCR), etc.)


Hugger Toobs part number 10010FLT offers a universal installation solution for all small block Chevy V–8 applications. (Headers sold separately)
Universal Block Hugger Toob, Fits 265–400 Engine, Connects Block Huggers to Existing Exhaust System, Heavy Duty 16–Gauge Mandrel–bent Tubing, "One Pair" Comes Complete With Flanges and Premium Gaskets.
Primary Toob Collector Size is 2.5" . Breaking in an engine with ceramic coated Headers WILL result in damage to the coating and will VOID all warranties. Ceramic coated Headers require several heat cycles to fully cure before they will withstand extreme heat. FlowTech recommends using cast iron exhaust manifolds or old Headers to break in new engines to avoid coating damage. Please call FlowTech tech service at 270-781-9741 for additional information regarding ceramic coated exhaust products.
Offers a universal installation solution for all small block Chevy V-8 applications
Designed to connect Block Huggers to existing exhaust systems
Built from thick, heavy-duty 16-gauge mandrel-bent tubing


text:
The Farrier Registration Council is removing a Cornwall shoer from its roster after he was convicted of physically harming a horse while providing hoof-care services.
Michael McNamara, 41, who has been a farrier for 24 years, pleaded guilty to causing unnecessary suffering to a protected animal after punching, kicking and striking the tethered horse with his nippers 18 times. The incident was recorded on closed circuit video. A judge issued a 6-month community order, a night-time curfew and banned him from working with horses for 3 years.
The FRC’s Disciplinary Committee reviewed the case after McNamara’s conviction and determined that it will remove him from the register. He is eligible for consideration for reinstatement in 2 years and 6 months. Restoration is not automatic, the committee states. McNamara will need to persuade the committee that “it is safe and appropriate to restore him to the register.” The findings and sanction are subject to appeal.
“The conduct displayed by Mr McNamara was in every way appalling and has inflicted damage on the reputation of the profession of farriery,” says David Hall, chair of the FRC. “Together with our colleagues in the profession, the regulator will redouble its efforts to ensure high standards of equine welfare, such that the public may have confidence in Registered Farriers and the important work they do.”
The Worshipful Company of Farriers “absolutely condemns” McNamara’s actions, according to a statement.
“The Company finds any such conduct completely unacceptable, be it towards other persons or the equine,” it states. “The Company wishes to emphasize its faith in the vast majority of farriers who do not conduct themselves in such a way and recognizes that most farriers behave in a professional and compassionate manner. It is important that the damaging actions of a small few do not detract from the excellent work carried out by the majority of farriers in the U.K. or bring the profession into disrepute.”

domain: law


Orion for Consoles Gaming Headset
For all round gamers; whether PC, Mac, Xbox 360 or PS3 games consoles, you can now buy a single headset for all applications. ROG audio team has applied its skills and created the new Spitfire II audio console which pairs with the Orion headset. Together they deliver 90% effective noise cancellation, while the headphone amplifier boosts the audio range.
The system is entirely plug-n-play so no drivers are required, and while powered by USB (available on both consoles) the cabling totals a generous 4 meters so you’ll never go short. The included 2.5mm plug specifically enables Xbox gamers to use the microphone for voice comms as well.
Clarity, control and continuous comfort
ROG Orion for Consoles features speakers driven by powerful 50mm neodymium magnets, which provide both punchy bass and crystal-clear sound detail. With noise-isolating 100mm ear cups surrounded by luxurious ultra-soft leather cushioning and a low 268g weight, Orion for Consoles is comfortable to wear for even the longest gaming sessions. Extensive lab testing by ROG has resulted in a headband with finely balanced clamping forces, meaning the Orion headset stays firmly and comfortably in place.
With the flick of a wrist, the retractable microphone gives gamers the freedom to issue commands or chat, while an inline control makes quick work of adjusting the volume or muting the mic.
Environmental noise cancellation and amplification
Orion for Consoles comes with ROG Spitfire II, a dedicated audio console with ENC and a headphone amplifier that’s tailor-made for the Orion headset. Powered by USB, this small box of tricks includes a built-in microphone that actively detects and counters environmental sounds such as speech and keyboard tapping, so teammates aren’t distracted in chat by noises that might be occurring around gamer – the Spitfire II’s ENC technology eliminates more than 90% of environmental noise.
Spitfire II also includes a headphone amplifier to deeper immerse gamers and provide a clearer sense of what’s happening on the battlefield.
Wide compatibility and made for gaming
ROG Orion for Consoles includes everything gamers require to get going, so the fun starts right away. The supplied RCA-splitter and USB cables enable instant connection to the most games consoles and computers, while the included 2.5mm jack cable allows Xbox gamers to connect their controllers to the Spitfire II for chat via the headset mic. No set up or drivers are required — just plug and play.
High-quality materials and thoughtful design choices ensure that Orion for Consoles is fit for the most demanding gamers. The headset’s braided cable is ruggedized for great durability, while its generous 2.5m length is further extended by Spitfire II’s own 1.5m cable, offering gamers very wide freedom of movement.
- 50mm speakers with neodymium magnet drivers
- 100mm noise-isolating ear cups
- Retractable microphone
- 4m cable (2.5m headset, 1.5m Spitfire II audio console box)
- Inline volume control and mute switch
- More than 90% environmental noise cancellation (Spitfire II)
- Built-in headphone amplifier (Spitfire II)
- 268g (headset)


text:
- Created by: peggy bradford
- Added: 2 Oct 2008
- Find A Grave Memorial 30270691
- Sponsored by peggy bradford
Find A Grave, database and images (https://www.findagrave.com : accessed ), memorial page for Jefferson Lemuel Bradford (28 Nov 1911–26 Jul 1958), Find A Grave Memorial no. 30270691, citing Rosewood Funeral Home and Cemetery, Humble, Harris County, Texas, USA ; Maintained by peggy bradford (contributor 46868614) .

domain: history


text:
Meet the Cast of “Body of Proof”
ABC's new drama “Body of Proof” premieres next Tuesday right here on ABC6, and tonight we introduce you to the rest of the cast.
Jeri Ryan co-stars as Chief Medical Examiner Kate Murphy. Also in the office is Windell Middlebrooks, who you might recognize as the “Miller High Life delivery guy,” and Sonja Sohn, who played a police officer on “The Wire.”
ABC6 Anchor Doreen Scanlon spent time getting to know the cast of the show, which was shot entirely in Rhode Island, and introduces us to Ryan, Middlebrooks, and Sohn.
“Body of Proof” premieres next Tuesday night at 10pm on ABC6.

domain: artistic


text:
My first colour font. My zistersoul, but now with awesome 2 layers and it's original own colours!This is a clone of zistersoul eYe/FS
I made a font inspired by art-deco, which I really love. To more universal capital letters, more decorative lowercase have been made, which can be varied by additional alternative characters (o e i).
This font has been almost 3 years in the making. Mostly because how tedius it was to keep copying the same "bricks" over and over. I'm glad it is finished enough to share. I may or may not (most likely) add more glyphs later.
With this font I am late to the competition, but I still love Art Deco a lot.This is a clone
This is a really conservative design, reminiscent of the early days here at FS when all the features didn't exist. Yet they are well hidden here. In smaller font sizes, db Katalog looks really balanced… but scale it up and it soon doesn't look so round anymore. Maybe that's what makes it so. Perfectly imperfect.
In any case, I am open to any suggestions for improvement.
Currently under fontstruction!
'Peranbu' means immense love.
My only entry for the Love comp made hastily at the last day. Mish mash of styles. Comments/suggestions are welcome. :)
This font came as a fluke off of another font I've been working on since 19 July 2018.
H was the hardest to get right.
& is just a flipped g.
All cap bold serif
kerned : Russian, Latin Basic, More Latin
I can't even type cyrillic extended, there are too many letters in latin extended, I don't have greek keyboard either
I wish I could make everything, but I'm a human after all (or am I?), I decided that this is good enough
Paris, Berlin, Moscow in the 1920s... Art Deco, Bauhaus, Konstruktivizm... Effervescent people! This font is for Sandrine, the woman with white hat in the picture, and for all the people who together built the world. See also ztefan and zergei.
Beeinflusst von Kem Weber (1889-1963)
A-Z on capital letters, alternate Q, S, X, and Z on their lower case positions.
I no longer publish samplers to illustrate my fonts as my samplers aren't wanted. It's nice that Meek publishes them for me; for competitions it seems important that members are made aware of contributions and I appreciate visually contributing and being part of the working community effort.
But I can't justify having to bother him with requests to publish my samplers that I designed only because I made a new weird font for my own reasons, hoping to get some (helpful) comments to point me towards improvements, additions etc.

domain: design


Beyonce and Jay Z took a stroll down memory lane by posing in front of the same elevator where Solange early decked him 8 years ago
Serena Williams announced she is retiring from tennis after the 2022 US Open tennis championships
British pop star Rita Ora is a married woman. The 31-year-old singer married Taika Waititi, 47
Legendary Motown songwriter Lamont Dozier has died at age 81. The news was confirmed by his son Lamont Dozier Jr.
Judge Mathis says Ashanti should hit Irv Gotti with a cease & desist for spilling intimate details
Kanye West finally deleted his "Skete Davidson" Instagram post following reports that the former SNL comic is in "trauma therapy"
New Jersey rapper Fetty Wap is back behind bars after his bail was revoked for threatening to kill a man on FaceTime
A man is going viral for kicking a woman out of his hotel room when she refused to have sex with him after he paid $5,000 to fly her out
British singer, actress Dame Olivia Newton-John has died after a long battle with breast cancer. She was 73
Joe Biden's $789 billion health, climate and tax bill includes $80 billion to hire 87,000 IRS workers


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text:
8 And all the elders and all the people said to him, “Do not listen or consent.” (ESV)
Scripture taken from The Holy Bible, English Standard Version. Copyright ©2001 by Crossway Bibles, a publishing ministry of Good News Publishers. Used by permission. All rights reserved. Text provided by the Crossway Bibles Web Service.
by Karl Koehler

domain: literature


CoronaFest Ladies Boyfriend Tee
Next Level Apparel Boyfriend Tee. Her vision of his shirt. This fabric is comfortable and lightweight. Colors are solid unless otherwise noted.
Fabric: Fine Jersey. 100% Combed Ring-Spun Cotton and 90/10 Cotton/Polyester in color Heather Gray / 32 singles. 145 grams/4.3oz.
Binding: Set in collar 1x1 baby rib.
Label: Tear Away
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text:
Not on display
- Stephen Gilbert 1910–2007
- Lithograph on paper
- Image: 420 × 360 mm
- Purchased 1987
P77187 Untitled 1949
Lithograph 420 × 360 (16 1/2 × 14 1/8) on machine-made wove paper 561 × 439 (22 1/16 × 17 1/4); printed by Jean Pons, Paris; not editioned
Inscribed ‘Stephen Gilbert 1949’ below image b.r. and ‘Epreuve d'artiste’ below image b.l.
Purchased from Noël le Gall, Neuilly-sur-Seine (Grant-in-Aid) 1987
In conversation with the compiler in Paris on 8 December 1988, the artist stated that the Scottish painter William Gear had encouraged him to experiment with lithography. William Gear (see entries on Gear T04995 and P77163) had been living in Paris since 1947 and was in contact with several other British artists there, as well as a number of School of Paris artists including Atlan, Poliakoff, De Stael and Soulages. Like Gilbert, who came to Paris in 1945, Gear was associated with the Cobra group. Gear introduced Gilbert to the lithographic printer and painter Jean Pons, whose print studio was at 128 rue de Vaugirard in Montparnasse in a cellar below a millinery shop owned by Pon's wife. Gilbert recalled, ‘I think he [Gear] said, “well I'm making a lithograph there with Pons and it won't cost very much and would you like to come?” ... no-one made much money then and the lithograph gave me a stock... At that time I only had my Cobra paintings which I couldn't sell and I didn't have a dealer’.
P77187 is black-and-white and the image, although abstract, is insect-like. It is clearly related to an untitled painting of 1948 (repr. Stephen Gilbert: Peintures de l'epoch COBRA et sculptures/structures en métal 1958–1984, exh. cat., Galerie 1900–2000, Paris 1984, p.17, no.8 in col.). The painting also depicts an insect-like form with a dark blue body and brilliant red wings. The triangular head, triangular eyes and mouth are typical of the fantastic imagery that Gilbert explored until 1950. It was images like these that attracted the attention of the Cobra group. For a discussion of Gilbert's ‘insect’ imagery and his involvement with Cobra, see the entry on T04933.
This entry has been approved by the artist.
Tate Gallery: Illustrated Catalogue of Acquisitions 1986-88, London 1996


A motorist has been jailed for three years after he knocked down and killed a cyclist while using his laptop.
Photographer Raymond Isherwood, 53, smashed into the back of chef Jake Gilmore, 19, at 34 mph as he headed home from work in Bath, Somerset, then drove off without even slowing down.
He later said that he had not seen Mr Gilmore because he was fiddling with the CD drive of his laptop, which was on his passenger seat.
Bristol Crown Court heard Isherwood, of Bennett Street, Bath, did not attempt to brake or stop as Mr Gilmore’s body flipped onto his car bonnet and over his roof.
Mr Gilmore was discovered by passers-by lying fatally injured on the road, Midland Bridge Road, and died in hospital hours later.
Isherwood abandoned his car in a park but police found a piece of his Golf and were able to connect the two.
He admitted charges of causing death by dangerous driving, failing to stop after an accident, perverting the course of justice and failing to surrender to police bail.
Isherwood, who had a clean license and no previous convictions, also pleaded guilty to production of cannabis, relating to 46 plants found by officers in his top floor flat.
Judge Michael Roach sentenced Isherwood to a total of three years in prison.
He said: ‘You thought only of yourself and you sought to hide what you had done.
‘Jake Gilmore was 19-years-old, he was in the prime of his life. He was a much loved son and brother.
‘The impact of his loss on his family and friends is profound. Their suffering will have been made worse by the callous way you left the scene.’


Giant African Snail Watch in Maine?!
I copied this off the maine gov.site. ALthough, I had never seen or have had any slug problems, this article caught my attention! Just wanted to share this with my Maine Garden Friends.
For immediate release Contact: Terry Bourgoin, 287-3891
SPEAR WARNS MAINERS TO KEEP WATCH
FOR GIANT AFRICAN LAND SNAILS
AUGUSTA - Maine Department of Agriculture, Food and Rural Resources Commissioner Robert W. Spear is urging the public to assist the Department in the hunt for giant African land snails (GALS) after recent seizures of this pest in Wisconsin, Illinois, Iowa and Ohio pet stores, exotic animal dealers and schools. The snails are a threat to human health and a potentially serious plant pest.
"We want to determine whether these snails are already in Maine and if so, collect them as quickly as possible," Spear said. "The discoveries in other states are of concern because the snails were being used at schools as part of science projects. Many of the schools received the snails as donations and were unaware of the potential threat to human health and plants. We want to make sure Maine schools are aware of the dangers associated with these snails," Spear said.
According to Terry Bourgoin, Director of the Division of Plant Industry, another mode of entry of the snail into the state is exotic pet stores. State officials are planning to spot check pet stores for this and other prohibited snails. Bourgoin encouraged any schools, education programs, pet shops or members of the public that might have these snails to immediately contact the Departments Division of Plant Industry at 287-3891 or the United States Department of AgricultureÂs Animal and Plant Health Inspection Service (USDA-APHIS) at 945-0479. Officials from one of these agencies will make arrangements to collect the pests from across the state. Though there are currently no known GALS in Maine, the potential for damage from this pest is high. For example, in 1966, a boy smuggled three snails into Miami, Florida as pets and his grandmother subsequently released them into her garden. Seven years later, more than 18,000 snails were found. It took 10 years and more than $1 million to eradicate the pest from Florida.
Scientists consider the GALS to be one of the most damaging snails in the world because it is known to consume at least 500 different types of plants, including most varieties of beans, peas, cucumbers, and melons. GALS are illegal to import into the United States without a permit, and currently no permits have been issued.
There are also public health concerns surrounding these snails, because they are known to carry a parasite that can cause serious diseases in humans, including a rare type of meningitis. These diseases can be transferred to humans by eating raw, undercooked infected snail meat or vegetables contaminated by the snails. Humans can also be infected by handling live GALS if the snailsÂ secretions contact mucous membranes in the eyes, nose or mouth. For questions concerning the health risks associated with GALS, the public can call the Bureau of Health at 800-821-5821.
The GALS are among the largest land snails in the world, growing up to 8 inches in length and 4.5 inches in diameter. Each snail can live as long as nine years, and contains both female and male reproductive organs. After a single mating session, each snail can produce 100 to 400 eggs. In a typical year every mated adult lays about 1,200 eggs.