text:
Chuy s jalapeno ranch dressing recipe
Recipes / Chuy s jalapeno ranch dressing recipe (1000+)
Steakhouse's Texas Ranch Dressing, ingredients: 1 pack Hidden Valley ranch dressing 1pinch
s Mexi Ranch Dressing, ingredients: 1/, 1 tsp chopped canned jalapeno slices, 1 tsp
Jalapeno Ranch Dressing. Jalapeno Ranch Dressing clips so here's a little clip that
several excellent recipes for them,Jalapeno Ranch Dressing Cajun Boogie Here's a little
s Day Menu (click on title to view recipe Jalapeno Ranch Dressing. Jalapeno Ranch Dressing
any color) chopped 2 tsp.'s minced garlic 1 shells Jalapeno Ranch Dressing (recipe below
CHUY'S JALAPENO RANCH SAUCE 1 whole jalapeno
Ranch Dressing Can’t Be Beat Buffalo Chicken Salad with Light Ranch Dressing
Ranch Dressing 1 (1-oz) packet of Hidden Valley ranch dressingof it. And that's a promise
While shrimp is marinating, combine jalapeno with ranch dressing mix and sour cream in a
Groups / Chuy s jalapeno ranch dressing recipe (0)
First course, main course or dessert salad recipes plus salad dressing are all welcome
flours etc and lot's of poultry stock , this group is for recipes that are cheap, but
Debbie's recipe swap, A place to share and swap recipes and ask cooking related questions
Delicious Indian Halwa’s, All Authentic Recipe's of Indian Halwa (A Yummy dessert)., main
s Favorite recipies. You know, the ones and beg her to make. Ya, those recipes you
Themes / Chuy s jalapeno ranch dressing recipe (0)
St. Patrick's Day party this year. We scoured the best looking recipes from throughout
S'mores Day! So lets celebrate with some unique s'more recipes and food. Pass the s
food critics? Try these recipes for your afternoon soiree when it's just too hot to cook
an old fashioned taste. Mom, and Grandma's recipes that go back years and years of family
you may want to wait on. And here's one last recipe for you: Mimosa: 1 part orange juice
Trusted Results with Chuy s jalapeno ranch dressing recipe
jalapeno ranch dip Submitted by Alexistx on Dec 09, 2009 ... Recipe Flash: get weekly recipes & food features. Tasting Notes: receive monthly wine ...
The perfect dip for your favorite vegetables or chips made from a delicious blend of sour cream, jalapeno peppers, cilantro, and ranch dressing. For a spicier dip, ...
I did not have any sour cream or dry ranch but I did the following ... because ever since having creamy jalapeno dressing at Chuy's in Texas, my wife and I ...
advanced recipe search. incredible slow roasted shoulder of lamb Submitted by johnom on ... 2. chuy's jalapeno ranch dressing recipe. 3. caraway chicken. 4. ...
Recipe Flash: get weekly recipes & food features. Tasting Notes: receive monthly wine ... 4. chuy's jalapeno ranch dressing recipe. 5. banana. 6. ethiopian ...
Recipe Flash: get weekly recipes & food features. Tasting Notes: receive monthly wine ... 1. chuy's jalapeno ranch dressing recipe. 2. shrimp pasta. 3. lemon bars. 4. wild mushroom ...
advanced recipe search. Crispy Lamb Spare Ribs with Honey CrÃ¨me FraÃ®che and Chopped Pea ... 2. chuy's jalapeno ranch dressing recipe. 3. shrimp pasta. 4. lemon ...

domain: food


text:
Auber by Hakwood
Premier 1 – Bis
The French White Oak floors with a proprietary colour and textural treatment by Dutch artisans, Hakwood, result in an internal canvas that references the nearby beach sand in the sunlight.
The buildings’ clean lines perfectly frame spectacular views of the beaches below.
The architects selected Auber, from the Sierra Collection by Hakwood to floor the majority of this Clifton residence.

domain: artistic


Cool rooms Melbourne
Melbourne Cool rooms & Commercial Refrigeration Engineers Designs Modular/Custom Cool Rooms, Industrial Freezer Rooms for Restaurants, Cafes, Hotels, Fast Food Retails, Hospitals, Food Manufacturers, Bars, Pubs and Clubs in cost-effective Cool Room for your business
Cool Rooms - Melbourne
Melbourne Cool room and refrigeration designs a whole procedure of decreasing the temperature and keeping it in a given area for the motive of chilling ingredients, maintaining certain materials. The reason for the calculation of cooling load is important selecting system components consisting of a compressor, condenser and so forth. Effectively and economically designing a cold room it is vital to achieving specialist advice from Melbourne cool room engineers.
Our Engineers however our patron must recognize the basic ideas so that it will guide the layout. With the bottom fee and, at the identical time, being able to fulfill the requirements availability of spare components in the unlucky event wherein the gadget breaks down, it needs to be serviced inside the shortest time feasible so the disruption of the flow of operation will be minimized. Place of the cold room in a groovy location inside a secure building at a secure site, with minimum risk of flooding or different herbal screw ups. if possible, find the cold room in order that it's far accessed directly from a drying room. This can mean that only pre-dried air can enter, and will lessen the construct-up of ice within the bloodless room. The bloodless room needs to be produced from a hundred-200mm thick pre-fabricated Styrofoam panels. The greater the thickness of the panels, the extra the initial fee of the structure, however the decrease the lengthy-time period strolling prices.
Aspects that make these rooms perfect:
Designing a cold room it is important to obtain specialist advice from Melbourne Cool room engineers however, our customer should understand the basic principles in order to guide the design
- Cost: The lowest cost and concurrently being able to consummate the requirements. The availability of spare components in the hapless event where the machine breaks down, it has to be serviced in the shortest time possible, so the disruption of the flow of operation will be minimized.
- Location: The cold room in a cool place within a secure building at a safe site, with minimum risk of flooding or other natural disasters. If possible, locate the cold room so that it is accessed directly from a drying room. This will mean that only pre-dried air can enter, and will reduce the build-up of ice in the cold room.
- Insulation panel types: The cold room should be constructed from 100-200mm thick Pre-fabricated Styrofoam panels. The greater the thickness of the panels, the greater the initial cost of the structure, but the lower the long-term running costs.
- Cold-room and freezer door heating: All cold room freezer room, it is necessary to have the door frame heaters installed and turned on constantly as the costs of maintenance. Door frame heaters are necessary as it prevents ice from forming around the door mechanism, and potentially causing the door to jam and lock up during attempts at opening them. Because of this, are such that the door frame heaters are twenty-four hours operation.
- Defrosting of cold-room: The best system is found in designing cold rooms is “Defrost on Demand” and this will be enabled to cut the energy costs. The basis of this function is to reduce the number of defrosts scheduled and defrosting will only carry out when the need arises such as when the air blower is blocked by ice.
- Floor Design: Our engineers use the best floor heating method of prevention requires heating cables run at around 400 – 600 mm spacing. We can design the layout and either supply the cable with layout plan, or complete the installation. Also as backups, dual circuits with one cable not connected are usually employed. The cable loading is very low preventing any form of cable deterioration however, most clients request dual circuits for the added peace of mind.
- We provide Facility for Hiring Cool Room, Portable Cool Room.


text:
Century Dictionary and Cyclopedia
- In Greek antiquity, an Athenian festival in honor of Dionysus (Bacchus), celebrated in the ancient temple of that god, called the Lenaion, to the south of the Acropolis. It was the second of the series of Dionysiac festivals, and took place during the month of Gamelion (part of January and February); it was the occasion of a procession, and of dramatic contests in both tragedy and comedy. See
Sorry, no example sentences found.
‘lenaia’ hasn't been added to any lists yet.
Looking for tweets for lenaia.

domain: library


text:
Anime and Manga Otaku 😉
My 3 fantasy novels:
1. Adult High Fantasy: Part 1 - Mysteries of the Ultimate War God (on-going)
2. Low Fantasy, Vampires (at inception stage)
3. Low Fantasy, Time Travel, set in Japan (on hiatus)
These will be the only fantasy novels I'll ever write. All 3 will be unique in its own way. Love is a genre in all 3, but the storylines will focus on other genres.
The rest of the novels will all be set in the real world and will have a common theme - Love and Romance.
Facebook Page: https://www.facebook.com/Ryū-no-Hono-108952000933961/
Meet my mascot, Shizu-san!
She's my very first digital art. Glad how she turned out! 😍
- JoinedApril 29, 2015


text:
Ube Halaya is a Filipino dessert made from a boiled ube or purple yam. This dessert dish is also used in pastries, ice cream and in halo-halo (Philippines dessert with mixture of shaved ice). Optional toppings includes brown latik (fried coconut cream) or grated cheese.
- 1 kilogram ube (purple yam)
- ½ teaspoon vanilla extract
- 1 small can (12 oz) evaporated milk
- 2 small can (28 oz) condensed milk
- 1 cup coconut milk
- In a bowl, heat 4 cups of water and boil the ube for 30 minutes. Set aside for 10 minutes.
- Peel and grate ube. Set aside.
- Heat the pan in a medium and add the coconut milk, condensed milk and vanilla. Stir well.
- Lower the heat and add the grated ube. Stir regularly for 30 minutes or until mixture is thick.
- Pour the evaporated milk, stir and let it cook for 15 minutes. Remove from heat.
- Pour the mixture in a baking dish or llanera mold. Refrigerate and let it cool.
To make it sweeten, sprinkle sugar on top of ube halaya before refrigerating it.
Instead of grating the ube, chop it and use blender.
Try and enjoy this Ube Halaya Recipe (Purple Yam Cake). Let me know what you think.


text:
Senthil Ganesh is an film artist who work in Indian film industry as playback singer and music producer. Check out Senthil Ganesh most popular works, listen to some of the latest Senthil Ganesh movies songs, Senthil Ganesh old films songs or Senthil Ganesh top hit songs from the movies. Senthil Ganesh profile including date of birth, age, place, images etc.
Senthil Ganesh Mp3 Songs
\"Onnuku Renda\" from Vantha Rajavathaan Varuven by Senthil Ganesh, V.M. Mahalingam, Sathya Narayanan. Released: 2019. Track 3 of 6. Genre: Tamil.
\"Danga Danga\" from Viswasam by Senthil Ganesh, Rajalakshmi. Released: 2018. Track 5. Genre: Tamil.
\"Chinna Machan\" from Charlie Chaplin 2 by Senthil Ganesh, Rajalakshmi. Released: 2018. Track 1. Genre: Tamil.
\"Paraak Paraak\" from Seemaraja by D. Imman, Senthil Ganesh. Released: 2018. Track 4 of 12. Genre: Tamil.


Stainless Steel Process Vessels
Since 1975, Pope has provided stainless steel process vessels to industrial and academic research laboratories and industries of all kinds. Beginning with standardized pressure vessels for holding and dispersing solutions, Pope over the years, also offered special vessels, reactors, process vessels and complete turnkey integrated processing systems. For years, Pope has been known and respected worldwide as the Solution Driven supplier of quality, innovative processing systems, reactors and standard pressure vessels.
Process Vessel Options
Sizes range from less than 1-liter to 1000 liters or more, with pressure from high vacuum to 500 psi, and with temperature ranges up to extreme ranges. Accessories and options are practically limitless. Pope’s application specialists work with clients from suggestions for basic vessel options to design of complex turnkey systems, and from general industrial to the critical high purity and pharmaceutical grade requirements.


IRAN though they had netted the most famous goal in their history – only to see it chalked out for offside.
The Group B minnows were convinced they had levelled during their defeat to Spain when Saeid Ezatolahi lashed a 62nd-minute shot past David De Gea.
It was a strike which sparked a mass pitch invasion from then Iran bench.
But their joy was cut short by an eagle-eyed linesman who had spotted Ezatolahi straying offside in the build-up.
Uruguayan ref Andres Cunha, who awarded a VAR-assisted penalty to France at the weekend, was once again happy to let the men in the TV studios make the big call.
And despite Iran’s protests to the contrary, the video replays backed the linesman’s judgement and lucky Spain were off the hook.
WE'RE RON THE BALL All the latest action, news, goals and gossip from the World Cup
Iran must now beat reigning European champions Portugal if they are to make it out of Group B and into the last 16.
Spain, the 2010 World Cup winners, just need a draw with Morocco to reach the knockout stage.
Fernando Hierro's side were frustrated by Iran throughout the game and were lucky not to concede earlier in the 53rd minute.
MOST READ IN WORLD CUP
Karim Ansarifard lashed a low shot into the side-netting which many fans inside the Kazan Arena thought was in.
Just a minute later Spain sprung up to the other end of the pitch and Diego Costa netted the winner.
And the former Chelsea striker knew little about it when as the ball ricocheted in off his knee.


As you might have guessed by now, I am a huge supporter of DFA records. I think they are pretty flawless. They never cease to amaze me. With that said, I am totally diggin' Shit Robot's two singles. The first is “Galaxy.” If heard in a club, it would killer. It is a damn good dance tune, very DFA. The other single is what gets me going called “Triumph.” Now I hate uplifting dance music. Jesus, I hate it. There is nothing worse than a bunch of dancers with their hands up in the build up while the DJ is doing the same. There are few sites that make sicker. Yet, I can’t help but feel some kind of spirit come out of me when I hear this song. The end is a true triumph. Indistinct vocals come in sung by an unknown person, more than likely James Murphy since he sings on Juan Maclean’s songs too. This song is too contagious. I had to listen to it over and over. I urge everyone to find this song. It is one of DFA’s finest moments.
I have a friend who is the world’s biggest Animal Collective fan. He is the type to get all the singles imported songs and whatnot. A while ago he had posted a video on a web page of mine. It was a solo performance by Animal Collective’s Panda Bear. It was an enjoyable song. I remember enjoying, but only watching it once. Then I see that Panda Bear has a song that you can listen to. This song is called “Bros.” I honestly can’t think of a more likable tune. It’s drenched in reverb and Panda Bear’s vocals just dive right into your heart. As I write this, I am listening to it in my third consistent play. It is utterly beautiful and angelic. If you don’t fall in love with this song, more than likely you have no soul.
Here is the link - http://downloads.pitchforkmedia.com/Panda%20Bear%20-%20Bros%20%28Edit%29.mp3
I just read today that the amazing Avalanches are coming back. Funny, all the artists I am mentioning in this post make very sunny songs. It is in the dead of winter, and the fountain outside of my apartment complex froze over yesterday, but I am really feeling some summery music right now. Anyways, they are back! Now I have total faith in them. See, DJ Shadow created the ultimate cinematic moody sample album. To redo it, is a very hard thing. Quite impossible. The Avalanches, on the other hand, made the ultimate sunny party sample- based album. One can do that again but even better. They say it is more hip- hop than the first one. That makes me even more excited. The first one had a lot of hip- hop elements, but there were a lot of house elements on it. So this is exciting news. Those two along with 3 Feet High and Rising are probably the finest completely sample based albums ever. The Aussies say the album is “so fucking party you will die.” I’m already shaking with anticipation. The Avalanches are quite perfect for the realm of sample- based artists. They make damn good music, it can be utterly moving, and they are about as carefree as one can get. That is what should be happening. They just are having a good time while still remaining innovators. Exciting times are ahead of us. This year has a great deal of potential.


text:
High Court has asked State officials to clarify the whereabouts of murder victim Mariyam Sheereen’s children to obtain their statement as her next of kin.
Sheereen was reported missing in December of 2009 and her body was found hidden under a pile of garbage at a construction site in Maafannu district of Male’ City in January of 2010.
Her boyfriend, Mohamed Najah from Ocean Villa in L. Kalaidhoo, was charged with her murder. He was accused of killing Sheereen, stuffing her body into a suitcase, taking a taxi, and dumping her body at the M. Angaagiri construction site.
He was granted an acquittal by the Criminal Court due to lack of sufficient evidence in 2015 – which was appealed by the Prosecutor General’s Office with the High Court.
The High Court has now concluded appeal hearings and is ready to issue its ruling.
The appeal proceedings are being presided over by Judge Mohamed Niyaz, Judge Shuaib Hassan Zakariyya and Judge Hussain Shaheed.
The court, on April 25, ordered the Prosecutor General’s Office clarify the whereabouts of Sheereen’s children to obtain their statement as her next of kin before the court issued its ruling.
The court scheduled a hearing this Tuesday to clarify the information.
Sheereen married three times and gave birth to four children. Her two eldest children are now adults, while the third eldest is now 16 years of age. Her youngest child died in 2012.
State’s prosecutors said this Tuesday that they sent a letter to the police for assistance to clarify the whereabouts of Sheereen’s children on April 30.
According to the prosecutors, her eldest child currently resides in London, UK. The prosecutors said the eldest was autistic, and the judges responded that autism cannot be taken as general grounds to establish a person not to be of sound mind.
Her second eldest child currently resides in L. Gan.
Her third and youngest surviving child is currently in critical condition following a motorcycle accident in L. Gan in August. He broke his back in the accident and is paralyzed from the waist down.
Her youngest child had died in a fire in Lh. Naifaru in 2012.
None of her children had been adults during the original trial, and their statement as Sheereen’s next of kin wasn’t obtained when the Criminal Court issued its verdict.
Judge Niyaz questioned the decision during this Tuesday’s hearing. He said that a son is considered a person’s next of kin as a general rule.
The Criminal Court had obtained statements from Sheereen’s mother and father as her next of kin. Her mother said she wished the court to apply the death penalty, while her father voiced indecision.
The death penalty is applicable for murder convicts if all next of kin wish the court to apply the principles of Qisas. Murder convicts will be issued a 25-year life sentence if even one next of kin doesn’t wish the death penalty to be issued.

domain: news


text:
Lisle, ILUSA 60532
January 18, 2014
I recently read Gone Girl by Gillian Flynn and have been wanting to read Dark Places by her as well before the movie comes to theaters.
You can never go wrong with sangria.
New to the chicagoland area and recently rediscovered my love of reading.

domain: literature


SAN DIEGO – A federal investigation into the financial practices of Rep. Duncan D. Hunter, R-Alpine, ranges from alleged campaign finance irregularities to bank fraud, according to a search warrant that was made public earlier this week.
An Inland Empire lawmaker is seeking to ramp up rare-earth mining throughout the United States to secure elements necessary to meet defense needs, rather than allowing foreign entities, mainly China, to dominate
the field of production.
RIVERSIDE – New and familiar names are on Tuesday’s election ballot, competing in congressional races throughout Riverside County. In the 36th congressional district, which encompasses the entire Coachella Valley – stretching east to Blythe and west to Hemet – Sen. Jeff Stone, R-Temecula, and accountant Stephan Wolkowicz are vying to displace Rep. Raul Ruiz, D-Palm Desert. Ruiz unseated […]
TEMECULA – An inland congressman’ bill seeking to strip federal funding from so-called “sanctuary cities” – which maintain policies shielding undocumented immigrants with criminal backgrounds from deportation – was approved today by the U.S. House of Representatives. “This legislation is about one thing – accountability,” said Rep. Duncan Hunter, R-Temecula. “When the arrest of an illegal immigrant is made, the federal […]


text:
I really love working with fresh flowers! They radiate elegance and perfectly compliment a simple cake.
Autumn, though some call it Fall because all the leaves fall off the trees, is my favorite time of the year. When I was in New York we would always take long autumn rides along the Hudson river overlooking New York City just to view the colors of the trees. Oh my goodness, there were bright reds, dark maroons, shades of brown, glorious golds, bright yellows, oranges, olive greens all set against that clear blue sky with a crisp “where’s my sweater?” breeze. But, in tropical Townsville where I currently live, that dreamy picture doesn’t really exist. Though this is the autumn/winter season here, the days are about 75-80˚F. Let’s be honest, palm trees are green and occasionally brown 24/7/365!!
My neighbor was celebrating her 5th Anniversary and requested a cake. She wanted to keep it simple so I suggested a few fresh flowers. Where I live has a big lawn with about 1/2 of it dedicated to a meticulously kept garden. In it grows everything from papau fruit to tomatoes; from lettuce to roses. I really enjoy the sweet scents that greet me every morning. The flowers on this cake are from a quick walk and snip, snip around the garden. Fortunately, I was able to capture the rustic colors of tropical autumn which makes me love this cake. It’s a product of the tropics that brought back great memories of the Big City.
About the cake: Chocolate layered with chocolate buttercream; covered in Vanilla buttercream.

domain: geography


A telehandler working on a project for Network Rail, in the UK, overturned into a canal last week, when the bank gave way.
The telehandler, a five metre/3,500kg JCB 535 from the Ardent fleet was apparently lifting material to be used on the project to shore up the nearby railway embankment. Reaching the line at this point is quite difficult, but a bridge – Stanton’s Bridge – carries a track from the main A419 London road south of Stroud in Gloucestershire.
We understand that the telehandler was lifting a section of a temporary bridge/pontoon that has been installed to cross the canal, rather than using the small bridge. As the machine took the load the bank collapsed causing it to overturn into the water. The operator was able to jump clear and was uninjured. It was fortunate that the telehandler was not facing in the opposite direction.
There has been some diesel and oil spill into the water, but measures have been taken to contain and recover it. A week on and the telehandler is still in the water.
We have asked Network Rail if it wished to comment. There is also the main contractor and a sub-contractor involved. We have their names but have yet to categorically confirm their involvement – or not. Network Rail has now responded, a spokesperson said: “On Tuesday 25 April, a telehandler operated by one of our contractors became unstable in muddy conditions and subsequently slipped into the canal in Stroud. Fortunately, no one was hurt during this incident. Specialist teams worked to remove the telehandler from the water and safeguard the environment.” We have also had confirmation now that the principal contractor is Sisk, and the subtractor is Ballycommon.
This looks like a classic case of operator error or perhaps more likely poor planning. Ww will update if and when we learn more.


text:
Welcome to December Daily® 2017 | Day 01.
I am so excited to be embarking on these daily stories once again. Really and truly. Let's do this.
Last year I started my album with a story about "paying attention" along with photos of each of the kids in a 2x2 pocket page. I loved including current photos of the kids at that time so I decided I wanted to begin this album with a photo that includes all of us along with details of our ages this year.
Here's a look at my Day 01 spread:
Today I paired a 6x8 photo of all of us with + the back of one of the gold foil tags to hold a little story.
As a reminder, here is what I had in my album for my Day 01 Foundation Page. Sometimes I only use what I had slipped in the pockets and sometimes I change things up:
Check out my overview video below to see how the page evolved from my foundation page to the end result.
I added one of the half-circles from the Main Kit + a cork #1 to the top of my 6x8 photo printed here at home using a Canon Pixma MG7720. I then ran that through my sewing machine to add the bit of red stitching along the top. Next to each person in the photo I added white number stickers from the kit + small star embellishments.
To add text to the half circle I used the December Daily® December Moments Stamp Set to create the curved text at the bottom the half circle.
Still loving the see-through lids on my inks - makes it super easy to see where you are stamping.
The second piece of my story today is the large tag that holds the story of our ages right now. I decided to use the back of one of the gold foil tags to hold the content of my story - see more below.
In the process video you'll see me make some mistakes and then some work-arounds to cover those up. My main issue today was not letting my ink dry (I should have just started with StazOn) before running it through the typewriter. No worries. I just cut out another tag and added the typewritten names first and the number stamping second.
I totally recommend cutting a new tag from white cardstock and working off that vs. working directly on the back of the gold foil tags.
On top of the tag I used the numbers from the December Daily® Weekly December Stamp Set to correspond with the number stickers I added to the photo + a 3x4 pattern card + a simple chipboard circle from the Main Kit. I used a Tiny Attacher to staple the chipboard in place (I gently peeled the pattern + cardstock back in order to not have the staple go through to the other side).
Here's a look at the two pieces of today's story outside the album:
Remember, your stories don't have to be long and complicated. Think about the kinds of stories you'd like to look back on in the future and the kind of information you'd like to have easily available (like the ages of your family members). Go tell those!
DAY 01 | VIDEO OVERVIEW
- December Daily® Main Kit 2017
- Brother Sewing machine
- We R Memory Keepers Typecast Typewriter
- Stamps: December Daily® Weekly December Stamp Set, December Daily® December Moments Stamp Set
- AED Inks: Pendleton, Black Butte
- Printer: Canon Pixma MG7720
- Tiny Attacher
- 6-Hole Punch
- Power Punch
- AED Precision Pen
SHARING FOR 2017
This year I'd love to invite you to leave a link in the comment section if you'd like to share your daily pages. As usual, please include a direct link to your blog post vs. a general blog address link.


text:
The banking sector was the Kingdom’s top hiring industry during the second quarter of 2016, according to a report released yesterday by online career portal Everjobs Cambodia.
The report shows that more and more of the country’s 200,000 university students graduating annually are starting their careers in the financial sector, reflecting the rapid growth of the industry, which now attracts more new hires than hotel and lodging services.
The findings were based on site traffic on the Everjobs portal during the second quarter, including job vacancies posted to the site and web searches by job-seekers. Everjobs did not indicate the sample size, but claims its website receives an average of 6,500 visits a day.
The greatest number of vacancies posted to the site between April and June were in the accounting/finance domain, particularly the banking industry, the report said.
The results reflect the rapid expansion of Cambodia’s financial sector, which is driving economic development, with the domestic credit market growing 27 per cent in 2015 on rising demand for consumption and construction, it added.
Casey Barnett, president of CamEd Business School, said the report matches a trend he has seen with his students, who are receiving job offers from established financial sector firms long before finishing their degrees.
“This matches my experience at my institute . . . where 44 per cent of our 4th year students [all of whom major in accounting and finance] have already been given offers by leading international banks and audit firms even though they won’t finish their studies until December, and the graduation ceremony won’t be held until April 2017.”
The Everjobs career report also notes a surprising and sudden increase in searches for part-time jobs.
“Gaining experience with a part-time job is one of the best ways to ensure employment after graduation and it is definitely something that our partner companies are looking for when recruiting new candidates,” stated Aliaksandra Iuchyk, the company’s marketing, communications and PR manager.
Contact PhnomPenh Post for full articlePost Media Co Ltd
The Elements Condominium, Level 7
Hun Sen Boulevard
Phum Tuol Roka III
Sangkat Chak Angre Krom, Khan Meanchey
12353 Phnom Penh
Tel: +855(0) 23 888 161 / 162
Fax: +855(0) 23 214 318

domain: finance


text:
Industrial Relations: Theory and Practice follows the approach established successfully in preceding volumes edited by Paul Edwards. The focus is on Britain after a decade of public policy which has once again altered the terrain on which employment relations develop. Government has attempted to balance flexibility with fairness, preserving light–touch regulation whilst introducing rights to minimum wages and to employee representation in the workplace. Yet this is an open economy, conditioned significantly by developing patterns of international trade and by European Union policy initiatives. This interaction of domestic and cross–national influences in analysis of changes in employment relations runs throughout the volume.
The structure has been amended slightly. Britain is placed straight away in comparative perspective before attention focuses explicitly on employment relations actors, contexts, processes, and outcomes. Each of the chapters is written by authorities in the field and provides up to date analysis and commentary. A spine of chapters from the preceding volume have been revised and extensively updated and new chapters have been added to refine coverage of issues such as the private sector and developing legal institutions.
Overall, a picture emerges of an economy that is in incremental and contested transition. The imperatives of ′globalization′ now infuse governance mechanisms that were once responsive principally to domestic agenda and employment standards are set now by the state that once were established through collective bargaining. It is this fragile and emerging model that will be tested significantly through sustained political and economic change.
List of Common Abbreviations.
List of Contributors.
1 Work, the Employment Relationship and the Field of Industrial Relations (Trevor Colling and Michael Terry).
Section One British Industrial Relations in Comparative Context.
2 British Industrial Relations: Between Security and Flexibility (Colin Crouch).
3 British Industrial Relations: The European Dimension (Richard Hyman).
Section Two Actors.
4 Management: Caught Between Competing Views of the Organization (Keith Sisson and John Purcell).
5 State, Capital and Labour Relations in Crisis (Jason Heyes and Peter Nolan).
6 Trade Unions: Power and Infl uence in a Changed Context (Melanie Simms and Andy Charlwood).
Section Three Contexts.
7 Public Sector Industrial Relations: The Challenge of Modernization (Stephen Bach).
8 Industrial Relations in the Private Sector (James Arrowsmith).
9 Multinational Companies: Transforming National Industrial Relations? (Paul Marginson and Guglielmo Meardi).
10 Industrial Relations in Small Firms (Monder Ram and Paul Edwards).
Section Four Processes.
11 Negotiation and Collective Bargaining (William Brown).
12 Employee Representation (Michael Terry).
13 The Changing Legal Framework of Employment Relations (Linda Dickens and Mark Hall).
14 Legal Institutions and the Regulation of Workplaces (Trevor Colling).
Section Five Outcomes.
15 Pay and Working Time: Shifting Contours of the Employment Relationship (Damien Grimshaw and Jill Rubery).
16 Industrial Relations and Economic Performance (Paul Edwards and Sukanya Sengupta).
17 Skills Policy and the Displacement of Industrial Relations: The Elephant in the Corner? (Ewart Keep, Caroline Lloyd and Jonathan Payne).
18 Equality and Diversity: The Ultimate Industrial Relations Concern (Deborah Dean and Sonia Liff).


text:
- Baked Goods
- Frozen Cookies
- Frozen Michael Cookies Dough
- Michaels Cookies Carnival Cookie Dough 1.3 Ounce
- Last products viewed
Michaels Cookies Carnival Cookie Dough 1.3 Ounce Product ID: OTF501989 Michaels Cookies
- "Thank you so much for the prompt service. This Jim Beam hot sauce is hard to find in local grocery stores for whatever reason. Thanks for carrying the product and please do not stop. Very satisfied customer!!!! GREAT JOB!
- "I was so happy to find Fabulous Fajita Marinade. It was easy to order and the shipping was fast. Best stuff ever!
Michaels Cookies Carnival Cookie Dough 1.3 Ounce
Product Id: OTF501989
Sold as: Case of 196Weight: 16 lbs
List Price: $108.20Savings: $23.35
Cookie Snackwell Chocolate Sandwich product id: OTC376294 Baked Goods
Our commitment to you
FoodServiceDirect.com is your source for items including Michaels Cookies Carnival Cookie Dough 1.3 Ounce! Try emailing or calling us at 757-245-7675 - we'll do our best to answer your questions. Count on secure shopping and dependable delivery from FoodServiceDirect.com. Thanks for shopping for Frozen Michael Cookies Dough with us!


text:
When you click on links to various merchants on this site and make a purchase, this can result in this site earning a commission. Affiliate programs and affiliations include, but are not limited to, the eBay Partner Network.
New to this. Either of these 1982 D pennies a small date. Both are copper.

domain: economics


text:
Amelia Earhart talk Sunday
CLINTON — “Amelia Earhart: Lost Legend,” will be presented by Cheryl Pula at the Clinton Historical Society, 1 Fountain St., at 2 p.m. Sunday, Feb. 11.
The program is free.
Amelia Earhart was and remains the most famous aviatrix in world history.
She disappeared on an around the world flight attempt in 1937, and no trace of her has ever been found. Pula’s program traces that flight, and offers some theories as to what might have become of her.
Pula is a retired reference librarian and a founding member of the New York Mills Historical Society. She is currently the New York Mills village historian. Pula has written, co-authored and edited several books and also shares her passion for history, giving lectures about a variety of topics.
12th annual Chili Bowl Luncheon Saturday in Old Forge
Drawing classes with Imundo start Feb. 20
Winter break events at Oneida library include Dinosaur Dig, crafts
History center offers book sale
- 4:00pm 02/14/1812th annual Chili Bowl Luncheon Saturday in Old Forge
- 4:00pm 02/14/18Drawing classes with Imundo start Feb. 20
- 4:00pm 02/14/18Winter break events at Oneida library include Dinosaur Dig, crafts
- 4:00pm 02/14/18History center offers book sale
- 4:00pm 02/14/18Park marks Douglass’ 200th birthday
- 4:00pm 02/14/18Two exhibits open in Hamilton
- 4:00pm 02/14/18Library hosts stargazing event
- 4:00pm 02/14/18Register for felting class by Feb. 23
- 4:00pm 02/07/18Clinton hockey history event Feb. 9
- 4:00pm 02/07/18Oneida library offers golf lesson, Valentine’s Day event

domain: hobby


text:
The Ethiopian government’s announcement in January that it would release jailed political leaders, some already convicted and others with ongoing trials, was music to the ears of many, including the prisoners’ families and friends, as well as human rights activists, but few thought it would come to pass. In the same announcement, the government also promised to shut down the notorious Maekelawi Detention Centre where most of the prisoners, and many others before them, were held and in many cases tortured or ill-treated. The government will not admit that most of the detainees, who it labelled “criminals”, were in fact held on false charges and simply for their political views and activities.
Well, to the surprise of the doubters, the government made good its promise, and has so far released more than 7,000 prisoners, including renowned journalist Eskinder Nega, who spent more than seven years behind bars, and prominent opposition figure Bekele Gerba.
But in a twist of events just a few weeks later, the high hopes raised by the government’s perceived move towards reform were dashed when it declared a state of emergency. This was amid heightened political tension and instability following a 3-day strike in the Oromia Region which disrupted transportation and flow of goods and services into the capital, Addis Ababa, and the resignation of Prime Minister Haile Mariam Desalegn.
This is a clear abuse of power by the Ethiopian authorities and serves as yet more evidence that the state of emergency is being cynically exploited for political ends.Seif Magango, Amnesty International’s Deputy Regional Director for East Africa, the Horn and the Great Lakes.
And Ethiopia continues to disappoint. On the night of 25 March 2018, Eskinder Nega was re-arrested, along with 10 other people. This is a clear abuse of power by the Ethiopian authorities and serves as yet more evidence that the state of emergency is being cynically exploited for political ends.
Already the serious human rights violations that many had feared and warned could result from this state of emergency have started materializing. On 10 March, Ethiopian security forces shot dead 10 people and injured 15 others in the southern town of Moyale, on the Kenyan border. This use of deadly force by the security forces, which the Ethiopian government has sought to explain away as a “mistake” due to incorrect intelligence of rebel activity by the Oromo Liberation Front, has already displaced thousands of people – with at least 8,000 of them fleeing across the border into Kenya.
Here are 10 facts about this state of emergency
- The current state of emergency, which was declared on 16 February 2016, is the second one in less than two years. The first one lasted 10 months, from October 2016 to August 2017. During those 10 months, the security forces arrested more than 26,000 people and committed serious human rights violations, including torture. This current one is already showing alarming signs of the same.
- On 21 February, the government issued a directive in which it outlined acts prohibited under the state of emergency and the punishments those found violating the directive will face.
- The directive imposes blanket restrictions on the right to peaceful assembly. In effect, all unauthorized protests are prohibited, yet the procedure for authorization of protests is vague. There is no clear guidance on what information is required or the procedure to follow in order to get permission to organise a protest.
- “Dissemination of any information criticising the state of emergency or any other similar activity”, both on social and traditional media, is also banned. So even simply discussing the state of emergency is risky and could land a person in detention if deemed critical of the state of emergency.
- The Command Post, the body overseeing the implementation of the directive, now has sweeping powers to shut down any media outlet to “safeguard the constitution”.
- The directive is rife with broad and vague phrases such as “communications with terrorist organizations and anti-peace groups” and “disrupting tolerance and peace”. This gives the security forces unfettered powers to clamp down on all expression of dissent, including peaceful dissent.
- Under the directive, security agents now have powers, even without a warrant, to arrest anyone suspected of breaching its restrictions. They also have free rein to search houses and vehicles without a warrant.
- Even schools are not spared. Security forces can enter schools and universities to stop any act they believe violates the rules established under the state of emergency and to remain there for as long as necessary. Prolonged presence of security forces in institutions of learning is not only intimidating to students but may also expose them to sexual violence and harassment.
- The directive also authorizes the Command Post to provide “rehabilitation education” to those detained for violating its terms. If what happened in the last state of emergency is anything to go by, this will lead to human rights violations against detainees, including torture. Under the previous state of emergency, many detainees were beaten, verbally abused and subjected to strenuous physical exercises during “rehabilitation”.
- The state of emergency is further constricting the already narrow space for freedom of expression, assembly and association in the country.
The above raise serious concerns about the protection of human rights during the state of emergency period. The Ethiopian government must reconsider the overly broad powers granted to the Command Post with a view to reducing them, and ensuring that any measures taken under the state of emergency do not violate the rights of the people in Ethiopia.
Join us, let’s tell the Ethiopian government to ensure human rights are protected during this state of emergency and that no more human rights violations are committed during entire period and beyond.

domain: law


text:
Join us in the Innovation Workshop for a month of game design! From putting your own twist on classic table top games to designing your own unique game, you make the rules!
Explore, expand, and invent games that utilize basic mechanics like pulleys, wheels, and levers.
Use die, spinners, and other props to create your own game of chance.
Take inspiration from our hands-on exhibits and turn them into a game for all our guests to play!
Learn the basics of classic games like chess and checkers that have withstood the test of time. Then adapt the game pieces to your invented rules and goals.
Using blocks that stack, code that runs, or even your mind that races, work on imagining a new world with its own gamified goals.

domain: design


LAST week Tory Chancellor Rishi Sunak unveiled the Tory offensive against the rights, conditions and wages of every worker.
This is the strategy behind the ‘kickstart scheme’ which aims at creating an army of 350,000 young people in a cheap labour scheme by paying employers the full amount of their minimum wage for 25 hours of work a week.
To give the employers time to organise dumping their workers and making use of this cheap labour force the Tories are even offering to pay them a £1,000 bonus to keep previously furloughed workers in their jobs until January 2021 on their existing wages and conditions.
When this ends the wholesale slaughter of wages and conditions will be launched against the dwindling number of jobs remaining.
Already in Sunday’s Telegraph paper an article commented that business needed ‘to free up the employment market’ and that ‘the best way to encourage firms to hire people is to make it easier to fire them’ as the only recipe keeping the profits flowing.
With unemployment estimated to reach at least 4 million as the furlough scheme ends the drive will be to super exploit the working class by beating them into submission.
The world has indeed been turned upside down from the days not long ago when Johnson pledged to ‘level up’ society, and end austerity. The elderly who were promised the everlasting protection of the triple lock guaranteeing pensions kept pace with inflation will soon find this abolished along with their free TV licenses.
Having been thrown out of hospitals and pushed into the life threatening environment of care homes without any protection from Covid-19, pensioners are discovering that as far as capitalism is concerned they are entirely dispensable.
This rapid transformation into its opposite under the impact of capitalist crisis is also seen in the international conflict between imperialism and the degenerated and deformed workers states of Russia and China.
Boris Johnson is due on Tuesday to set a deadline for the Chinese company Huawei to be thrown out of the UK’s 5G network and Tory MPs are demanding that Huawei equipment be removed from all parts of the mobile network.
What a change from the days when China was Britain’s best friend ever, when Tory prime minister David Cameron could happily sit down in a pub for a pint and fish and chips with Chinese president Xi Jinping back in 2015 and proclaim it marked a ‘new golden era’ between the two countries.
Since 2010, under Tory governments, British capitalism has welcomed with open arms Chinese investment and last year the UK was the second largest recipient of direct Chinese foreign investment.
All this changed in the past few months. The massive international crisis of capitalism is driving this transformation as the capitalist class are turning in desperation to a war on two fronts – a war against the working class at home to smash trade unions and war against the degenerated and deformed workers states of Russia and China to restore capitalism.
Lenin always insisted that there is no hopeless situation for imperialism, that it will always turn to war for survival and rely on reformist Labour leaders to rescue it from revolution at home. Lenin’s warning must be taken seriously today – capitalism will not simply collapse and give way without a fight, it must be overthrown by the working class in a socialist revolution.
This requires the building of the revolutionary leadership of the WRP in the UK and building sections of the Fourth International throughout Europe and the Americas to overthrow capitalism and imperialism.
This will create the conditions for the Soviet and Chinese workers to carry out politcal revolutions to bring back rule through workers soviets and set the scene for the victory of the world socialist revolution.
This is the way forward for the working people of the world. Build the WRP and sections of the Fourth International worldwide to organise to smash capitalism and imperialism and secure the victory of the world socialist revolution.


text:
Knox County, Tennessee school bus contractor Bill Gentry says "he's ruining my credibility as an operator, as a company.
Bill Gentry is frustrated, watching video trasmitted from one of his buses stolen earlier this week.
Bill says "you've got some yahoo, jumps it and steals it for a joyride."
Bus 78 sat on the Gentry lot in Fountain City until, Gentry says, "one day we went out there to retrieve it to do some work on it this week and the bus wasn't there."
One item the driver probably hasn't noticed is a security camera located on the bus. It starts recording anytime there's an abrupt stop. That video is then sent to the bus office and apparently this driver has hit the brakes a whole lot.
Manager Greg Gentry has reviewed numerous clips. The video shows a man driving around Fountain City several times.
Greg says "it looks like he's pulling into a station on Broadway. He's taking a break."
Bill says "you've got a crazy driver out there."
Gentry hopes the ride ends before school starts.
Bill says "a child could be picked up by some derelict. It could be used a some kind of a weapon."
The bus was eventually found in Dooley, Georgia, south of Atlanta.


text:
Adam Harding will ply his trade for Elite League outfit Manchester Storm in 2016/17 (PIC: Nicky Pearce).
Adam Harding has brought his time as a Swindon Wildcats player to an end (PIC: Nicky Pearce)
Neil Liddiard signed a new one-year deal with the Cats over the weekend (PIC: Nicky Pearce).
Neil Liddiard has put pen to paper on a new contract with the Wildcats.
Neil Liddiard has agreed to return to the Cats for a second straight season.
Aaron Nell and Neil Liddiard celebrate a goal during the 2015/16 season (PIC: Nicky Pearce).


text:
Sian is known for her quirky movement style and creative approach to her work, constantly pushing boundaries in contemporary and urban movement.
Her dance training began in Geelong where she trained in a variety of styles. She then continued her studies full time in Melbourne with Jason Coleman’s Ministry of Dance completing a Diploma of Elite Dance.
Sian has choreographed music videos for artists ‘Bree Tranter’, ’The Gaze’ and ‘Teagan’ and worked as an assistant choreographer to Zoee Marsh (Spring Awakening) and Freya List (Rent). Aside from choreographing, Sian has performed for artists ‘Woodes’ and ‘Eden Swan’, and at various events including ‘Melbourne Fashion Week’, ‘The Australian Open’, ‘Southern Cross Austereo’ and the‘VRC Young Members Ball’.
Constantly pushing for more opportunities to create and experiment, Sian’s work has been seen at many Underground events, as a showcasing choreographer at Grounded: Battle of the Arts and in the Boss Dance Company shows ‘Alpha’, ‘Conquest’ and ‘Four’.


text:
"I really did try to get away from the typecast"
Actress Preeti Jhangiani has said that she was typecast as a ‘bechari bahu’ (helpless daughter-in-law) and that she found it hard to distance herself from that image.
The actress achieved fame in the 2000 film Mohabbatein.
Preeti also revealed that audiences did not accept her taking up edgy roles and that some of her fans to date still only want to see her in sarees.
She explained that she wanted to expand her acting horizon but her fans did not support her.
“Some people did try to change it. I did Victoria No 203 where I played a negative character; I absolutely loved it.
“I still use photos from that photoshoot, I am in so much love with them.
“I really did try to get away from the typecast but the audience didn’t accept it.
“Even if I wanted to do a kissing scene, they wouldn’t accept it, though I did have a kissing scene in a Rajshri video too.
“In films, I feel, people just refused to accept it.
“I still have diehard fans who tell me not to wear anything but just saree kind of things.”
Preeti went on to reveal that some people still refer to her as the ‘Chhuimui girl’ or the ‘Mohabbatein girl’.
She said that she is fine being called these two names.
However, during her career, Preeti asked filmmakers to offer her other roles as she did not want to play the victimised daughter-in-law.
While she was given different acting roles, Preeti Jhangiani admitted that the stereotypical role still sticks with her.
“People still call me the ‘Chhuimui girl’ or the ‘Mohabbatein girl’.
“I am stuck with these two tags and I am fine with it.”
“Midway into my career, I was like, ‘Can you please offer me something else?’ I didn’t want to play the bechari bahu (helpless daughter-in-law) anymore.
“I was not denied roles but I was completely typecast.”
Preeti is now married to Parvin Dabas and the couple have two boys.
She said that in real life, she is not like the characters she played in films.
Preeti added that her parents were “quite forward-thinking” and that she didn’t even own a “salwar-kurta”.


text:
US 3804827 A
Description (OCR text may contain errors)
Unite W35 Pat-3m 3804 s27 l-p n ARABINOFURANOSYLCYTOSINE 335'- CYCLIC PHOSPHATE AND DERIVATIVES THEREOF Roland K. Robins, Santa Ana,and Robert A. Long, Costa" Mesa, Califi, assignors to ICN Pharmaceuticala-Ino,
No Drawing. Filed Feb. 28, 1972,. Ser. No. 230,127
Int. Cl. C07d 51/52 "13 Claims US. Cl. 260-211.5 R
ABSTRACT OF THE DISCLOSURE BACKGROUND OF THE INVENTION l- 8-D-arabinofuranosylcytosine (ara-C) is described and claimed in US Pat. 3,116,282 to Hunter as active against microorganisms, including viruses. Its antiviral and anti-. tumor activities, as since reviewed by Cohen, Introduction to the Biochemistry of D-Arabinosyl Nucleosidesfif Progr. Nucleic Acid Res. 5, 1-88 (1966), have been well, established. For example, ara-C has-been shown tofbe eflective in the treatment of herpes kerati'tis in experi: mentally infected rabbits, and also in man. In clinical studies, Chow et al., Antimicrobial Agents and Chemotherapy (1970) 214, found ara-C highly eifectiv'e in the treatment of a number of other herpes virus infectious, including herpes simplex encephalities. With ,the expressed aim of influencing the mechanism of action, transport or cross-resistance phenomena of ara-C, Wechter prepared a number of dinucleoside phosphates containingara-C [1. Med. Chem. 10, 762 (1967)] and first produced the phosphateof ara-C as an intermediate -(See US. Pat. 3,300,478) to those compounds, as by phosphorylation of l 3 D (2',3'dibenzoylarabinofuranosyl) N benzoylcytosine followed by base hydrolysis and debenzylation. Cytotoxicity and antiviral activity of ara-C was compared to that of the 5'-phosphate and a large number of the dinucleoside phosphates by Renis, who concluded in Cancer Res. 30, 189 (January 1970) that the activity .of phos:
phorylated derivatives of ara-C was due toara-Qitself,
which became available after enzymatic hydrolysis. Renis and others, as reported in J. Med. Chem. 10, 777--(l96. 7 found ara-C more active against herpes virus. than any of its 2'-, 3,'- or 5'-phosphates, and suggestedcleavageofi those phosphates and the dinucleoside phosphates to free ara-C to be an a priori requisite to their biological activity. The 5'-phosphate of ara-C was shown byschrecker and Golden, Cancer Res. 28, 802 (1968). to b'cdcphosr phorylated prior to uptake by tumor cells. The i2'; 5 cy l i phosphate of ara-C has been prepared by' we chtjer, who in J. Org. Chem. 34, 244 (1969) reports it tobe'devoidof' the antiviral activity associated with the parent nucleotide.
The sense of the prior art, then, is that ph'os]; 11olryilfatijon,
of ara-C gains no activity advantage, acting instead to diminish activity to a degree depending upon the ratg,
at which a particular phosphate ishydrolyzed to the free nucleoside at the cell surface. I
. BRIEF SUMMARY THE INVENTION A According to this invention, there are provider! asanti viral" agents ara-C 3',5'-cyc1ic phosphates, i.e., compounds of structure lm m.
wherein R is hydrogen or C -C acyl and is hydrogen or halogen, ar1d physiologically acceptable salts thereof.
" The preferred compound of the invention, ara-C 3,5'
cyclic phosphate was found to be superior to ara-C itself against herpes simplex encephalitis in vivo studies, and is cytotoxic to tumor cells in vitro. The invention also provides bioactive derivatives arising from substitutive operations; on the aglycon of that compound, as well as novel precursors for those derivatives. .These and other objects andsadvantages of the invention will be apparent from the toregoing and from .the detailed description which follows.
DETAILED DESCRIPTION OF THE INVENTION 1 fi-D-arabinofuranosylcytosine-3',5-cyclic phosphate (ara-C 315" cyclic Phosphate) is P P from cytidylio acid 2(3') mixed isomers laccording to the following reaction scheme:
1 0 N 0(- -O I bit By the method of Michelson, J. Chem. Soc. 3655 (1959), a suspension of 1 in water is solu bilized with tributylamine and ethylchloroformate added. Dehydration permits cyclization, yielding the tributylamine salt of the 2',3'-cyclic phosphate (2). O ,2'-anhydrocytidine 3'-phosphate (3) is next formed according to the method of Nagavary, J. Am. Chem. Soc. 91, 5409 (1969), by taking to dryness the product of heating 2 with trimethylsilyl chloride in pyridine and tributylamine. Treatment of 3 with alkali or bicarbonate opens the anhydro bond, affording the 3'- phosphate 4, which may be cyclized in pyridine with N,N'- dicyclohexylcarbodiimide (DCC) to obtain the desired ara-C 3,5'-cyclic phosphate 5.
The invention also comprises ara-C-3', '-cyclic phosphate analogs bearing halogen in the 5-position, as well as intermediates therefor. 5-iodo, -chloro, and -bromo analogs may be approached by direct halogenation at the election-rich 5-position of the cyclic nucleotides of the invention with reagents appropriate to the desired end, e.g., iodomonochloride in diethylacetamide, N-chlorosuccinimide (NCS) or N-bromosuccinimide (NBS), and bromine. The S-fluorinated cyclic nucleotide should result from reaction of the N,2'-diacetyl-blocked cyclic nucleotide with trifiuoromethyl hypofluorite, followed by deacetylation. Preferably, however, the compound is ob tained according to the method of M. I. Robins et al., I. Am. Chem. Soc. 93, 5277 (1971) for S-fiuoro cytidine arabinoside, i.e., ara-C-3'-phosphate is reacted with CF OF in CCl F to yield 5-fluoro-ara-C-3'-phosphate, which is in turn cyclized with DOC to yield the desired product. Corresponding 5-bromo and 5-chloro cyclic nucleotides may be obtained by reaction of the 3'-phosphate under similar conditions with, e.g., bromine, NBS or NCS. Ara-C analogs bearing halogen in the 5-position have been shown to possess antiherpes activity, e.g., Renis, Cancer, supra, the 5-halogenated analogs of the 3,5'- cyclic phosphate of ara-C can be expected to display similar activity, especially in view of our finding that S-fluoro ara-C-3',5' cyclic phosphate and its S-fluoro ara-C-3- phosphate precursor exhibit antiviral activity.
The invention also encompasses a number of other derivatives of the principal compound, which may be prepared according to the following scheme, 0 being acyl and AP p-D-arabinofuranosyl 3',5'-cyclic phosphate:
NH, 0 0 I II N I HN HN O=L O=L 0-L N N N AP AP 0-CH 0 KAcO/ (5H (7) is 01 SCH; l l l 4- OrL 0=L O=L N N N lp 11 l? With reference to compounds 6 and 9, it will be understood that equilibrium obtains as between their isomers:
(H) 0H 3 SH li e II respectively, and characterization of their distinctive groups as, e.g., hydroxyl" and mercapto is not to be taken as particular to one or the other isomer of the respective compounds.
Deamination of 5 with NaNO yields l-B-D-arabinofuranosyl)-uracil-3',5'-cyclic phosphate 6. Acylation of 6 in pyridine, as with acetic anhydride, provides the acylblocked analog 7. Treatment of 7 with SOCI dimethylformamide in refluxing chloroform provides the corresponding acyl-blocked 4-chloro analog, which upon deacylation yields 1-fi-D-arabinofuranosyl-4-chloro-Z-oxopyrimidine 3,5' cyclic phosphate 8. Nucleophilic displacement of chloro in this compound with sodium hydrogen sulfide or sodium methylmercaptide affords, respectively, l-p-D-arabinofuranosyl 4 thiouracil-3',5'-cyclic phosphate 9 or 1- 3-D-arabinofuranosyl-4-methylthio- 2-oxo-pyrimidine-3',5'-cyclic phosphate 10. The bioactivity of the latter two compounds is discussed infra.
Preferably, however, compounds 9 and 10 are successively obtained by direct reaction of 5 and H S according to the procedure of Ueda, Tetrahedron Letters, No. 27, pp. 2507-10 (Pergamon Press, 1971), affording 9, from which compound 10 may be obtained by alkylation.
The cyclic nucleotides may be acylated at the 4-amino group and at the 2'-hydroxyl with C -C acyl groups, preferably C -C acyl groups, as by reaction with appropriate acid anhydrides or acid halides in base. Sutherland et al., Biochim. et Biophys. Acta 148, 106 (1967) have demonstrated that acylation of cyclic nucleotides enhances cellular transport. Moreover, acylation of the 4-amino group is efiicacious in reducing the possibility of enzymatic deamination. Ara-C is known to undergo enzymatic deamination in vivo with concomitant loss of biological activity. While the corresponding 3,5'-cyclic phosphate itself appears more resistant to deamination than the free nucleoside, as is discussed hereafter, that resistance may be further enhanced by converting the cyclic nucleotide and its analogs to corresponding N -oxides, as by treatment with meta-chloroperbenzoic acid. The N -oxide 0f ara-C itself has been prepared by others. R. P. Panzica et al., J. Med. Chem. 14, 259 (1971).
As will be apparent to those of skill in the art from this, disclosure, the compounds of the invention may be employed in the form of their physiologically acceptable salts, e.g., ammonium, sodium and triethylamine salts prepared by neutralizing the 3,5'-cyclic phosphate with the appropriate base.
D. Gish et al., in J. Med. Chem. 14, 882 (1971) report O ,2'-anhydrocytidine to be active in vitro against herpes virus, and that the compound was markedly superior to ara-C itself in increasing life span of L1210 leukemic mice. 0 2'-anhydrocytidine 3,5-cyclic phosphate prepared by dehydration of ara-C-3',5-cyclic phosphate with POCl in ethyl acetate at reflux can be expected similarly to mimic if not improve upon the activity of cyclic nucleotide per se. An alternative route to the anhydro compound involves activation of the 2'-hydroxyl of ara-C-3,5-cyclic phosphate with, e.g., a mesyl or tosyl leaving group, as by reacting the cyclic nucleotide with mesyl chloride or tosyl chloride, followed by treatment with strong base such as NaOH, securing 2-oxo displacement of the leaving group (and therefore the anhydro cyclic nucleotide).
'Parallal tests with ara-C and ara-C-3',5'-cyclic phosphate showed them to be equally cytotoxic to KB, H.EP. #2 and HeLa tumor cell monolayers, but significantly, the cyclic nucleotide was less toxic toward non-tumor cell lines tested as well.
EXAMPLE 1 1-;3-D-arabinofuranosylcytosine-3',5'-cyclic phosphate (5) 1-B-D-arabinofuranosylcytosine-3'-phosphate (5.77 g., 17.9 mmole) and 4-morpholine-N,N-dicyclohexylcarbosamidine (5.24 g., 17.9 mmole) were dissolved in anhydrous dimethylformamide (100 ml.) by heating to reflux for 5 minutes. The solvent was removed in vacuo. Anhydrous dimethylformamide (50 ml.) was added and the solvent again was removed in vacuo. The resulting foam was dissolved in boiling dimethylformamide (150 ml.) and the resulting solution was added dropwise over a period of 105 minutes to a vigorously refluxing solution of dicyclohexylcarbodiirnide (2.1 g., 101.0 mmole) in anhydrous pyridine (4.5 1.). The solution, following the addition, was refluxed '60 minutes and then a vertical concentrater was attached and the solution was concentrated with heating in vacuo to a volume of approximately 400 ml. Water (400 ml.) was added to the cool solution followed by ether (600 ml.). The insoluble dicyclohexylurea was removed by filtration, washed well with water (200 ml.) and discarded. The aqueous layer of the filtrate was reduced to dryness. Ethanol (100 ml.) was added and removed in vacuo. The resulting gum was dissolved in water (150 ml.), ether (200 ml.) was added and the resulting dicyclohexylurea was removed by filtration. The aqueous layer was acidified with 12 N HCl to pH 1 and stirred for 5 minutes. Concentrated base (NaOH, 3 N) was added rapidly to obtain pH 6. The resulting solution was applied to a column (50 cm. x 6 cm. dia.) of Schleicher and Schuell standard grade diethylaminoethyl cellulose in the bicarbonate form. Elution was carried out using a linear salt gradient with 0.025 M triethylammonium bicarbonate, pH 7.5 (2 1.), in the reservoir and water (2 l.) in the mixing chamber. This gradient was followed by a linear salt gradient of 0.075 M triethylammonium bicarbonate pH 7.5 (2 1.), in the reservoir and 0.025 M triethylammonium bicarbonate pH 7.5 (2 1.) in the mixing chamber. Fraction (20 ml.) were collected at 3 min. intervals, the elution of products being followed spectrophotometrically at 254 mp. l-fl-D- arabinofuranosylcytosine-3,5-cyclic phosphate appeared in fractions 160-323. The combined fractions were concentrated to dryness, the solid residue was taken up in water. (100 ml.) and the solution evaporated to dryness to remove residual triethylammonium bicarbonate. The resulting solid was dissolved in water (100 ml.) and applied to a column (30 cm. x 4 cm. dia.) of Dowex 50W x 8 (H+ form). The elution of nucleotides was followed spectrophotometrically, with fractions (20 ml.), being collected. l-fl-D-arabinofuranosylcytosine-3',5'-cyclic phosphate appeared in fractions 26-60. After removal of the solvent in vacuo, the resulting solid was recrystallized from water to yield 0.645 g. The filtrate from the crystallization was freeze dried to yield 1.257 g. Total yield 1.902 g., 34.8%. An analytical sample was prepared by recrystallization from water and dried in vacuo at 110, M.P. 294-298 decomp.
Analysis.-Calcd for C H N P (percent): C, 35.39; H, 3.96; N, 13.76. Found (percent): C, 35.43; H, 4.04; N, 13.62.
Ultra-violet absorption: pH 1, A 277 (e 14,200); pH 11, k 268 (42 10,400), 236 (e 8,550); pH 7, A 268 (6 10,400). PMR dataspectra obtained on a Hitachi 60 spectrometer; chemical shifts are reported downfield with an internal standard of SDSS, 2,2-dimethyl-2-silapentane-S-sulfonic acid taken as 0 p.p.m.; solvent was D 0 with NaOR added to dissolve. Coupling constants are in brackets. H-6 6 7.62 ; H-1' 6 6.39.,;H-S 6 6.06 . Electrophoresis pH 7.2 phosphate, 1500 'v., reference uridine -phosphate; for II, one spot, R =0.72for uridine-3',5'-cyclic phosphate, R=0.70;
6 EXAMPLE 2 1-p-D-arabinofuranosyl-uracil-3', -cyclic phosphate (6) A solution of 1-(B-D-arabinofuranosyl)-cytosine-3',5'- cyclic phosphate sodium salt (0.700 g., 2.14 mmol) in 60 ml. AcOH--H O (5:1) was heated in an oil bath at 60, and NaNO in portions of 60 mg. was added every hour until the UV of the solution exhibited absorption maximum at 262 m at pH 1. The solution was then concentrated to dryness in vacuo, followed by addition and evaporation of EtOH. The residue was dissolved in H 0 (20 ml.) and applied to a column (60 milliequivalents) of Dowex 50 w x 8 ion exchange resin H+ form. Water eluted the uracil nucleotides first, this was followed by a separate peak containing some cytosine nucleotide. Fractions containing the uracil nucleotides were combined, and applied to a column packed with Sleicher and Schuell standard grade DEAE cellulose bicarbonate form (200 ml. wet volume). Elution was carried out with a linear gradient of H 0 (1.5 l.) and 0.125 M triethylammonium bicarbonate solution (1.5 1.). 3 UV absorbing peaks were eluted from the column, the fractions (39-62, 20 ml. each) representing the 2nd peak were combined and concentrated to dryness in vacuo.
The residue was dissolved in H 0 (20 ml.) and this solution was passed through a column of Dowex 50 w x 8 H form (40 milliequivalents). The eluate was con centrated and lypholized to give an amorphous powder, 0.293 g., 45%. A portion was crystallized from EtOH for analysis.
Analysis-Calculated for C H N PO (percent): C, 35.30; H, 3.62; N, 9.15. Found (percent): C, 34.38; H, 3;30; N, 9.02.
Ultraviolet absorption: pH 1, A 262 (6 7,260); pH 11 A 260 (e 6,000). PMR spectrum in D 0 with SDSS internal standard: H-6 5 7.68 [7.5]; H-1' 6 6.36 [7.0]; H-5 8 5.9 [7.5].
Paper electrophoresis at pH 7.2 phosphate buffer, 1 spot only.
' EXAMPLE 3 l-(B-D-arabinofuranosyl)-4-thiouracil- 3', -cyclic phosphate (9) A solution of l-(fl-D-arabinofuranosyl)-cytosine-3,5'- cyclic phosphate (0.915 g., 3 mmol) in 9 ml. of H 0 was placed in a steel bomb and cooled in Dry Ice-acetone bath. A mixture of pyridine (12 ml.) and liquid H S (24 ml.) was added and the bomb was heated at 60 for 48 hours. After cooling to the bomb was opened, allowed to come to room temperature, and the contents were concentrated to dryness in vacuo, followed by ad dition and evaporation of EtOH. The residue was dissolved in H 0 (80 ml.), the solution filtered and applied to a column packed with Sleicher and Schuell standard grade DEAE cellulose (450 ml. wet volume) bicarbonate form. Elution was carried out with a linear gradient of H 0 (2.5 l.) and 0.2 M triethylammonium bicarbonate solution 2.5 liter), the UV of the eluate was monitored continuously at 254 mp Fractions -154 (20 ml. each) representing the 3rd and major UV absorbing peak were combined and concentrated to dryness in vacuo. The residual yellow powder was dissolved in H 0 (40 ml.) and this solution was passed through a column containing 40 milliequivalents of Dowex 50 wx8 ion exchange resin H+ form. The eluate was concentrated in vacuo, and EtOH. was added and evaporated several times in order to remove residual H O. The residual yellow pow-- der was dried in .vacuo to give pure l-(B-D-arabinofura- Analysis-Calculated for C H N 0 PS (percent): C, 33.54; H, 3.44; N, 8.69. Found (percent): C, 33.85; H, 3.40; N, 8.74.
7 Ultraviolet absorption: pH 1, A 330 (8 21,800); pH 11, A 317 (6 21,600); H O, A 330 (e 23,200). PMR spectrum in D with SDSS internal standard: H6
Paper electrophoresis in pH 7.2 phosphate buffer at 1500 v. reference N -(fl-D-arabinofuranosyl)-4-thiouracil-3'- phosphate, one spot, Rf=0.48.
EXAMPLE 4 1- fi-D-arabinofuranosyl) -4-methylthio-Z-oxo-pyrimidine- 3',5'-cyclic phosphate ammonium salt To a solution of l-(fi-D-arabinofuranosyl)-4-thiouracil- 3',5'-cyclic phosphate (0.322 g., 1 mmol) in 5 ml. of Me0H--H O (8:2) was added conc NH OH dropwise until pH was adjusted to 11. Methyliodide (1 ml.) was added, and the solution stirred at room temperature for 5 hours, then kept at 5 for 12 hours. Crystalline NH I was removed by filtration, the filtrate was concentrated to dryness in vacuo. The residue was dissolved in H 0 (25 ml.) and applied to a column of Sleicher and Schuell standard grade DEAE cellulose bicarbonate form (150 ml. wet volume). Elution was carried out with a linear gradient of H 0 (1 liter) and 0.18 M triethylammonium bicarbonate (1 liter). Fractions 2854 (20 ml. each) representing the 2nd and major UV absorbing component were combined and concentrated to dryness in vacuo. The white solid residue was dissolved in H 0 (20 ml.) and passed down a column of Dowex 50 wx8 ion exchange resin, NH form. The eluate was concentrated to dryness and crystallized from EtOH, crystals were dried at 110 in vacuo, 0.165 g., 47%, MP. dec. 245.
Analysis.Calculated for C H N O PS (percent): C, 33.99; H, 4.56; N, 11.89. Found (percent): C, 33.71; H, 4.61; N, 12.03.
Ultraviolet absorption pH 11, k 303 (e 14,900); H O A 303 (e 15,150). PMR spectrum in D 0 with SDSS internal standard: H6 6 7.80 ; H5 6 6.63 ; H1' 6.34 ; CH 6 2.52.
Paper electrophoresis in pH 7.2 phosphate butfer at 1500 v., one spot only.
EXAMPLE 5 5-fluoro-1-;8-D-arabinofuranosylcytosine-3 '-phosphate To a suspension of 1-fi-D-arabinofuranosylcytosine-3'- phosphate (1.615 g., 5 mmoles) in methanol (50 ml.) was added 1,5-diazobicyclo-[5.4.0]undec-5-ene (0.760 g., 5 mmoles) and the resulting mixture was stirred until all solid had dissolved. The solution was reduced to dryness in vacuo. The resulting foam was dissolved in methanol (200 ml.) and cooled to approximately -78 in a Dry- Ice-acetone bath. To this cold solution was added a solution of trifluoromethylhypofiuorite (1.56 g., mmoles) in trichlorofluoromethane 50 ml.) After stirring at --78 for 90 minutes, nitrogen gas was bubbled through the solution to remove excess reagent and the solvent was evaporated under reduced pressure. The resulting solid was treated with triethylamine in 50% aqueous methanol (100 ml.) for 16 hours. The solution was evaporated to dryness in vacuo, water (30 ml.) added and removed in vacuo. The solid was dissolved in water (50 ml.) and applied to a column of Dowex 50 W x 8 (200 ml., 100-200 mesh). The column was developed with water and the appropriate portion of the eluent was reduced to dryness in vacuo. The solid was recrystallized from ethanol-water to yield 0.602 g., 35.3%.
Analysis.-Calcd. for C H N O PF. /2 H O (percent): C, 30.85; H, 4.00; N, 12.00. Found (percent): C, 30.52; H, 4.30; N, 11.74.
Ultraviolet absorption: pH 1, A 289 (e 12,100); PH 11, 76 280 (6 8,850), 237 (e 8,160); H O. A 280 (6 8,850), 235 (6 8,500).
PMR spectrum in D 0 with SDSS internal standard: H6,
8 6 7.98 [6.5]; H1, multiplet 6 6.19, no H5 signal. Fluorine spectra: D 0 with NaOD added, CCl F external standard 6 166.16 p.p.m.
S-fiuoro-1-fl-D-arabinofuranosylcytosine-3,5'-cyclic phosphate 5 fluoro-1-/3-arabinofuranosylcytosine 3 phosphate (1.100 g., 3.23 mmoles) and 4-morpholino-N,N -dicyclohexylcarboxamidine (0.945 g., 3.23 mmoles) were dissolved in warm pyridine (50 ml.). The solution was reduced to a dry foam in vacuo. This foam was dissolved in dry pyridine ml.) and added dropwise over a period of 90 minutes to a refluxing solution of DCC (3.09 g., 15 mmoles) in pyridine (1,500 ml.). The solution was refluxed a total of 4 hours. The product was isolated from a DEAE cellulose column utilizing the same procedure as for isolation of N -fi-D-arabinofuranosylcytosine-3,5-cyclic phosphate to yield 0.193 g.
Analysis.-Calcd for C H N O PF. 1.5 H 0 (percent): C, 30.85; -H, 4.00; N, 12.00. Found (percent): C, 31.10; H, 4.05; N, 11.89.
Ultra-violet absorption: pH 1, 1 289 (6 8,330); pH 11, 280 (6 6,300), 240 (e 6,480); H O, x,,,,,, 280 (e 6,300), 237 (6 6,650).
PMR spectrum in D 0 with SDSS internal standard: H-
6, 6 7.78 [6.5], H-1', doublet of doublet, 6 6.35 [6.5 and 1.5], no H5 signal.
1 'fi-D arabinofuranosylcytosine-3,5'-cyclic phosphate in KB cell culture studies exhibited inhibition of herpes simplex virus ranging from 67% to (CPE) with concentration correspondingly ranging from 1.0 to 100 mg./ml. (320 CCID /ml.). With primary mouse embryo cells inhibition ranged from 25% to 100% with concentration correspondingly ranging from 1.0 to 32 mg./ml. (32 CCID /ml. In each case, the compound was but slightly toxic at 1000 mg./ml., while in the case of the ME cells the compound was non-toxic at 1.0-10 mg./ml. In each case virus rating was 1.4, as determined by the virus rating (VR) method of Sidewell et al., Appl. Microbiol. 22, 797 (1971). V.R. 1.0 is indicative of definite antiviral activity, V.R. of 0.5-0.9 is indicative of moderate antiviral activity, and VR 0.5 suggests slight or no apparent antiviral activity. Other in vitro tests with vaccinia, pseudorabies, myxoma and murine cytomegalo viruses demonstrated marked OPE inhibition, indicating ara-C-3',5-cyclic phosphate to have equal, if not higher, activity than ara-C itself against DNA viruses of the Herpesvirus and Poxvirus families.
In addition to the above, 1-/3-D-arabinofuranosylcytosine-3',5'-cyclic phosphate exhibited significant activity against herpes keratitis infections in experimentally inoculated rabbits.
Most interestingly, the 3',5' cyclic phosphate proved markedly superior to ara-C in the treatment of mice intracerebrally infected with 10 LB 50 herpes simplex virus. 40 mg./kg. of compound was administered intracerebrally 6 hours after infection. Ara-C did not increase the number of survivors relative to the saline-treated controls, whereas ara-C-3',5'-cyclic phosphate increased that number by 7. On the other hand, ara-C increased mean survival time substantially more than did the cyclic compound, permitting the conclusion that its ultimate failure to enhance survival stemmed from enzymatic degradation not suffered by the cyclic nucleotide.
The antiviral activity of the S-fluoro nucleotides of the invention appears from Table I:
TABLE I N oncancerous pseudo rabies 1 Compound 1 Cell line: RK-13.
Myxoma 1 Compound 9, the thiouracil analog, has exhibited properties associated with immunosuppressive activity, as determined by measuring its effect upon uptake of tritiated thymidine (i.e., DNA synthesis) into trichloroacetic acidprecipitatable biopolymers. Compound 10, the methylthio analog, has displayed activity in vitro against HeLa and KB tumor cells, proving equal in effect to ara-C-3,5'-cyclic phosphate and superior to ara-C itself as against the latter cell line.
1. A compound selected from the group consisting of (1), compounds of structure HNR l x O=L -01-1 0 N w 0- I compounds of structure N 1 CH3 O=L and physiologically acceptable salts thereof.
9. A compound selected from the group consisting of (1) compounds of structure oi l tip wherein R is hydrogen or C C acyl and Y is selected from the group consisting of amino-, hydroxyl-, chloro-, mercapto-, and methylthio-, and (2) physiologically acceptable salts thereof.
10. An N -oxide of a compound according to claim 9.
11. 1-p-D-arabinofuranosyl-uracil 3,5' cyclic phosphate.
12. l-fi-D-arabinofuranosyl 4 thiouracil-3',5'-cyclic phosphate.
13. l-fi-D-arabinofuranosyl 4 methylthio-Z-oxo-pyrimidine-3',5'-cyclic phosphate.
References Cited UNITED STATES PATENTS 8/1967 Wechter 260--21l.5 R 8/1969 Hanze et al. 260-2115 R OTHER REFERENCES Smith, et al., Jour. Amer. Chem. Soc., vol. 83, pp. 698-703, 1961.
JOHNNIE R. BROWN, Primary Examiner US. Cl. X.R.

domain: chemistry


Introducing the Sharp BP50C36 Colour Essentials Series
Sharp BP50C36 is the perfect solution for businesses looking to take their communication to the next level. At Datasharp i-Solutions Ltd, we’re committed to offering cutting-edge technology that helps our clients work smarter and stay ahead of the competition.
Designed to cater to the diverse workstyles of today’s modern workforce, the Colour Essentials Series allows workers to seamlessly collaborate and securely share information across their office environment. Microsoft Teams integration and enhanced cloud services streamline communication and boost productivity for hybrid workers.
Some of the exciting new features include a 100-sheet duplexing reversing document with double feed detection and auto-skew correction, flexible paper handling that supports media up to 300 gsm, and a smart controller design with auto-firmware checks. The machine is always ready in seconds thanks to its walk-up motion sensor feature.
With multi-layered security protocols like system integrity checks at startup, firmware attack prevention, and optional Bitdefender antivirus software, businesses can rest assured that their data is protected.
Users will enjoy a responsive experience with the enhanced 10.1″ touchscreen and simplified data entry thanks to a retractable keyboard. Collaborative connectors for Microsoft Teams, Google Drive, and Dropbox make sharing information a breeze.
Sharp OSA technology provides a secure, intuitive user experience for effortless, integrated workflows. With optional Wireless LAN support for 5GHz and energy-star certification, the Sharp BP50C36 Color Essentials Series leads the way in providing businesses with state-of-the-art document systems that enable them to unlock their growth potential. Trust us – at Datasharp i-Solutions Ltd, we are dedicated to delivering reliable managed print services, IT Services, and Audio Visual Solutions for businesses of all sizes with our team of national field service engineers and dedicated tech centre.
All of our devices are backed by our manufacturer trained engineers and technical support personal. Our Managed Print Services not only support your chosen device but also your document workflow and processes. We monitor all of our devices live in real time for immediate response and reporting for service conditions, supply usage and replenishment orders. We specialise in not just providing a print device for your business but a solution for your total document requirements.


text:
The cytoplasm of bacteria is the colloidal matter containing water, mineral compounds, proteins, lipids, carbohydrates, etc.
Multiple ribosomes are present in cytoplasm being the sites of protein synthesis. Bacterial ribosomes have 70S sedimentation constant in ultra-centrifugation (30S for small subunit and 50S for large one). A cluster of 50 to 55 closely related ribosomes is known as polysome. The ribosomes and polysomes are linked to the cellular membrane.
The cytoplasm also contains autonomous genetic structures – small circular DNA molecules of plasmids and episomes. They encode the synthesis of variety of substances (toxins, microbial enzymes and many others).
Various kinds of inclusions are stored in the cytoplasm. Among them are volutin and starch granules, lipoprotein bodies, pigments, accumulations of sulfur, microcristals of calcium, etc.
Volutin granules are of sizes to 0.1-0.5 μm. They are located in cytoplasm on the ends of Corynebacterium diphtheriae rods; also they can be found in the yeasts and other microorganisms.
Volutin granules contain metaphosphates. According to Neisser’s staining method volutin granules stain blue, vegetative part of bacterial cell stains brown-yellow. Lipoprotein droplets are accumulated in cytoplasm, when bacteria grow on rich nutrient media. They are actively used by microbial cells in starvation conditions. Microscopical methods for their detection include Sudan stain.
Similar manner starch granules can be stored and next consumed by microbial cell in case of unfavourable situations. They are detected by stain with Lugol’s iodine solution.
The cytoplasmic membrane encases the whole contents of bacterial cell. It is the innermost layer of bacterial envelope usually of 5-10 nm in thickness. The membrane is generally organized like phospholipid bilayer with embedded membrane proteins. Bacterial membranes lack sterols except mycoplasma cells.
Cytoplasmic membrane produces a selectively permeable barrier that provides controlled transportation of water, electrolytes, and various nutrients into bacterial cell for its vital activity. The transfer of water and ions creates osmotic pressure within bacterial cell.
The membrane carries the vast number of receptors with diverse functions. They recognize the variety of signals from the environment; signal transmission results in changes of bacterial metabolism.
Multiple membrane enzymes perform biological oxidation in bacteria. Also they support biosynthetic reactions for building of bacterial components (proteins, nucleic acids, etc.) and actively govern bacterial trans-membrane transportation.
Membrane invaginations or mesosomes are the nearest mitochondria analogs of bacterial cells. They harbor oxidative enzymes of electron transport chain thus providing energy for bacteria. Mesosomes actively participate in the growth of bacterial cells and their reproduction.
The best method for study of bacterial cytoplasmic membrane is electron microscopy.


Relatively than studying from what occurred, Trump is encouraging Russia and doubtlessly others to do extra of the identical. This opens up each candidate – and perceived Trump rival – to assaults from hostile overseas powers.
It’s unclear whether or not the FBI has an ongoing counterintelligence investigation into President Trump. But when they do not, they might wish to revisit why not.
It’s unclear how a overseas authorities would contact Trump with out different US authorities officers realizing – presidential communications are alleged to be on official units that protect communications, and sometimes members of the White Home workforce pay attention to presidential calls or at the very least get transcripts.


text:
I needed this today. Maybe someone else did, too. ❤
The mess and the glory...
The space is holy, I think. I'll let you know in time, if it becomes clear.
I think my greatest moments of anxiety, ambivalence, and insecurity have been a result of my failure to make a firm decision about a guiding choice.
Hope for the enduring winters of life...
From splashes to rip tides, PTSD can affect lives in many different ways.
Dear Christians friends... You're not doing it wrong.
Thank God for His grace. When my mind tires from trying to find the hidden picture in the cacophony of my intricately complicated and beautiful life, I just fall into the arms of my Abba - Creator of the Universe and Lover of my soul - and listen for his still and calming voice lighting the path directly in front of me.
"I feel like He is telling me that my identity isn't in my healing, just like my identity wasn't in my being sick..."

domain: psychology


text: A study of changing cropping pattern for a period is important to understand the shifting nature of the composition of crops in the crop sector and the economic and non-economic factors that cause the cropping pattern change. Over the last quarter of a century of post-Bangladesh years, cropping patterns tilted towards MV variety of crops both in Kharif and Rabi season. Crop diversification has been taking place in the Rabi season. Growths of HYV Aus and wheat areas have been diminishing since the decade of the eighties. Local varieties of rices have been declining and got negative cropping pattern changes including jute, barley, mustard and chilli throughout the whole period of 1971/72-1993/94. It was observed that real output price change and cropping pattern changes had little relationship. Improved technology of production (seed-fertilizer technology) had rather influenced cropping pattern changes. Researches on varietal improvement and production technology should get priority to enhance higher returns from favourable cropping pattern changes and crop mix.

domain: agronomy


text:
Stansted Maple Manor Park & Ride reviews
We strongly encourage our customers to give feedback and share their experience of parking at Stansted. If you have used Stansted Maple Manor Park & Ride and would like to tell others about it, please submit your review. Thank you!Write a Review
Here are some comments that our customers have made about Stansted Maple Manor Park & Ride
Maple Manor Park & Ride was reasonably fast and convenient for parking at Stansted. I would use it again. Make certain to allow adequate time for the shuttle to the airport. It is on demand, but if you arrive while the shuttle is currently out, you must wait for the next trip.
April 25, 2016
Easy to find, with prompt and cheerful service - and so much better than using a vast car park and waiting for the shuttle bus (for which I would have paid more).
April 15, 2016
Horrendous directions to the parking place. The post code provided took us to a village in the middle of nowhere. Then the directions from the motorway when we eventually made it back to the main road sent us off in the wrong direction. Nearly missed our flight.
March 30, 2016
Would definitely use Maple Manor Park & Ride again. Very helpful and efficient service!
March 29, 2016
When I returned to the car, I found the passenger wing-mirror, pushed in, it had some white paint on it, so must have been in contact with either another vehicle, or a wall, I have wiped the paint away, and fortunately the mirror isn't broken. I also had need to use the toilet, which was pretty smelly, and grubby, there was a dirty mop sitting in some putrid water, and the sink, floor was also very dirty. The cost of nearly £40 for 5 nights, at the cheapest rate, I thought also a bit steep, as I have stayed near Gatwick for £27 for 7 nights. Other than that, the chap was prompt in turning up to take us back to the parking area from the air-port.
March 15, 2016
Efficient service. Quick transfer to and from the airport. The car was waiting for us on our return from the airport, ready to go!
March 14, 2016
Service for drop off of vehicle okay - ie. quick and immediate shuttle mini-bus to Stansted airport terminal. However, pick up procedure not clear - for a moment the printed instructions appeared to say wait for your vehicle to arrive. However, minibus picks you up and transports you back to carpark. The wait was about 25 minutes. Also, I had difficulty in understanding the person on the phone for pick up instructions, due to background noise and accent. Overall though, I would use service again, provided remains cheaper than counterparts.
March 8, 2016
A very well run process, the parking is ideally set minutes away from the terminal. the return procedure was equally timely giving you ready access to your vehicle and the A120 or M11.
This was the first time we have used this car park and I will definately use it again.
My only comment is that the car park signage on the approach is non-existant and needs to be improved.
March 7, 2016
The service was excellent - quick and efficient. Good directions to get to the site. Within 5 minutes of arriving at the site, I was in a minibus on my way to the terminal building.
When I landed back at Stansted, I phoned up and was told that the minibus would be outside the terminal building in approx 5 minutes. The minibus was there waiting for me. My keys were handed over as soon as I arrived at the and my car was just a few yards away from the office. There was even brown paper on the floor on the driver's side so that there were no foot marks left on carpet.
Within 30 minutes of landing at Stansted, I was on the A120 starting my drive to Norwich.
March 2, 2016
Easy drop off and transfer and on return, although there were several people waiting to be picked up at the airport, minimum wait for transfer back to car park.
Easy collection on arrival there.
March 2, 2016

domain: travel


text:
Mumbai: Alia Bhatt has all the reasons to be elated and thrilled. The young and very talented actress has struck a mammoth movie deal with her mentor Karan Johar. Daughter of veteran filmmaker Mahesh Bhatt and actress Soni Razdan, Alia, who was launched by Johar in Student of the Year has proved her mettle as an actress since her debut.
And now, she is all set to star opposite heartthrob Ranbir Kapoor in Dharma Productions’ Brahmastra, a fantasy adventure to be presented in three parts.
The film is apparently Johar’s most ambitious ventures so far. Directed by Ayan Mukerji, known to be a very dear friend of Ranbir, Brahmastra will also feature Amitabh Bachchan.
Alia took to Twitter to share her excitement.
— Alia Bhatt (@aliaa08) 11 October 2017
This will be Alia’s first film with Ranbir. She has shared screen space with Varun Dhawan, Sidharth Malhotra, Randeep Hooda, Arjun Kapoor, Shahid Kapoor and Shah Rukh Khan so far. And it will indeed be interesting to see her chemistry with Ranbir on screen.
Alia was introduced as a typical Bollywood heroine by Karan Johar in her debut film. But the pretty girl stumped the audiences by her performance in Imtiaz Ali’s Highway. She swept people off their feet by putting up an awe-inspiring performance in Udta Punjab.
She has had a string of hit films to her credit while Ranbir hasn’t had a great time at the Box Office. Ae Dil Hai Mushkil was the only film among his last few releases - Besharam, Tamaasha, Roy, Jagga Jasoos - to do a decent business at the BO.
And hence this mega-budget trilogy may infuse a new lease of life into RK’s professional graph.
RK is an actor par excellence. All he needs right now is a blockbuster to his credit. We can’t wait for this one. What about you?

domain: celebrity