You might have heard that ceiling fans aren’t as effective as air conditioners. Or maybe you think air conditioners will cost you a fortune in power bills? The truth is that both ceiling fans and air conditioners have great benefits. The best choice for your home or office will depend entirely on your needs and may, in fact, be a combination of both.
Initial outlay – which is cheaper to buy?
Ceiling fans are certainly the cheaper option to install, with the initial outlay starting at just a few hundred dollars per room. Single split system air conditioners start from $1500, fully installed by a qualified refrigeration mechanic and electrician.
Keep in mind you won’t need a separate air conditioner for each room, whereas the cost of ceiling fans will be multiplied by the number of rooms you want to cool in your home. You can reduce the overall cost of installation by packaging your air conditioner and ceiling fan and having them all installed at the same time.
Running costs – are ceiling fans really cheaper than air conditioners?
There is no doubt that ceiling fans are extremely economical, operating for as little as 1c per hour!
Modern air conditioners are a little more expensive to run, but they’re certainly much more cost effective than those old boxy units, which chewed through power in the summer months. Pairing an air conditioner with a ceiling fan will keep the air circulating freely throughout your home and reduce the need to have your air conditioner on a higher setting, reducing your overall power bill.
Which one keeps you cooler?
If you want to bring the temperature in your home down a few degrees quickly, you’ll need an air conditioner. However, ceiling fans are ideal for those humid, stuffy days where you just want a breeze to move the air through the house but the temperature is fine.
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CONTEMPORARY CLASSIC OF B&D! The Hollister School for Girls is a private college for wayward daughters of the very wealthy and aristocratic. New to the school from England is Headmaster Michael, who brings with him stern yet tried and true methods for dealing with troublesome young ladies. His expertise and knowledge of the ways of corporal punishment is precisely why he was hired on by Chancellor Meredith. In this spellbinding novel you will follow the journey of some of the school's more rebellious young women as they navigate their way through this maze to emerge better citizens. Here is another unique excursion into the world of b&d from the award-winning author Powerone.
See more books from this Author
Published September 10, 2005
by Sizzler Editions.
Erotica, Literature & Fiction.

domain: literature


text:
Teachers who knew Boston Marathon bomber Dzhokhar Tsarnaev as a child described him as a hardworking youngster who seemed to have a bright future. Catheryn Charner-Laird, his third-grade teacher, said Tsarnaev was just learning English after moving to the U.S. from Russia in 2002 and cared very much about his school work. She said he 'always wanted to do the right thing.' Other teachers said he got nearly all As in his classes, made friends easily and was an excellent soccer player. Scroll down for video An undated photo shown at the Boston bomber's trial on Wednesday shows brothers Dzhokhar, left, and Tamerlan Tsarnaev. Dzhokhar was convicted of the marathon bombings that killed three and injured more than 260 spectators in April 2013. Tamerlan Tsarnaev died in a firefight with police days after the bombings Boston Marathon bomber Dzhokhar Tsarnaev extends his middle finger to a security camera taken in his jail cell three months after the terror attack on Boston. The … [Read more...] about Teachers say Boston Marathon bomber was ‘kind as a child and a straight-A student’ as judge suspends trial until Monday because juror is unwell
English teachers in japan
Parents are being urged to ignore the latest school league tables, after "chaotic" changes to tests in England.Final year primary pupils sat a raft of tough new tests in the summer. Only 53% of pupils passed, compared to the 80% who passed easier tests in 2015.Primary heads leader Russell Hobby said ministers had stressed the scale of the changes meant comparisons could not be drawn on previous years' results.The government said schools had responded well to the higher demands.Pupils at 14,930 state primary schools sat the new national Sats tests in reading, writing and maths - the "Three-Rs"; they also sat a spelling and grammar test.Schools are then ranked by these results to enable parents to narrow down the choice of schools for their children. English primary school tables Department for Education website Sorry, your browser does not support this tool. Please visit the Department for Education. Join the conversation - find us on Facebook In the run up to and during … [Read more...] about Ignore school league tables, head teachers urge parents
Drivers applying for a minicab licence in London will have to pass a written English exam, including a 120-word essay, after Uber lost a high court battle with the capital’s transport authority. Mr Justice Mitting, presiding over the case, admitted that the requirement could see 40,000 drivers over three years either fail the test or be deterred from applying for a private hire vehicle licence. But he said Transport for London had no reasonable alternative to the exam, which lasts two hours, costs would-be drivers £200 and does not apply to black cabs. Uber said it would appeal against a verdict that the company’s London head, Tom Elvidge, called a “deeply disappointing outcome for tens of thousands of drivers who will lose their livelihoods because they cannot pass an essay writing test”. “We’ve always supported spoken English skills, but writing an essay has nothing to do with communicating with passengers or getting them safely from A to … [Read more...] about Uber loses court case to block English-language written test in London
Intelligence and dedication are the most important things when it comes to a child's academic success, but scientists now claim being able to manage their emotions is a crucial third. Emotional intelligence — the ability to restrain one's feelings — has been linked to better school performance in children and teens, according to a new study. It found that no matter the students age, those who are better at managing their emotions perform better academically and achieve higher grades. Scroll down for video Emotional intelligence — the ability to restrain one's feelings — has been linked to better school performance in children, according to research from Australia. Those that can't cope with their own or others' emotions struggle more in school (stock) Dr Carolyn MacCann from the University of New South Wales said: 'Although we know that high intelligence and a conscientious personality are the most important psychological … [Read more...] about Being smart and hardworking is not enough – emotional intelligence is also vital for academic success, scientists claim
Professor Sir Richard Evans, historian, president of Wolfson College, Cambridge Gove presided over the disintegration of our school system; he opened up teaching to untrained people in state schools, because he had contempt for professional educationalists. The restoration of professional teaching in our schools must now be an urgent priority. I'll remember him as someone who tried to dumb down the teaching of history by eliminating the teaching of skills and converting it to a mindless rote learning of a slanted patriotic version of events. With the history curriculum, he set up an elaborate consultation process, then after it was complete he ditched it and came up with his own half-baked ideas. And of course everyone rejected it. Fortunately, his attempt to foist his own rather ignorant and partial version of history on to the national curriculum was one of his many failures. Tom Sherrington, headteacher, King Edward VI grammar school, Chelmsford When you met him he was always … [Read more...] about Michael Gove: ‘bogeyman’ or ‘the greatest education secretary ever’?

domain: news


Atlona Technologies’ AT-PR082
This unit is a unique 8-input 2-output Professional Video Scaler designed to work as a switcher and scaler for multiple Audio/Video sources and displays.
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- Audio Volume Control for the audio outputs
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- Worldwide Power Supply - 100-240V.
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- Atlona 8-input 2-output Professional Video Scaler (AT-PRO82)
- Instruction Manual
- IR Remote Control
- Universal AC/DC power adapter: 12VDC
Instruction Manual (PDF, 0.8MB)


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For the record
Catch and release: Hospital charts fish hook removals
Law Enforcement Blotter: Thursday, July 27
Eastbound I-94 on-ramp near Garfield to close today
Police chief, sheriff set up timetable for adding body cams
Talented field returns amid Resorters changes
Infanger, Berberich earn Diamond King Awards
Alexandria's Schultz competes on national level in Brazil
Parkers Prairie adds cross country, hockey this school year
Junior Legion state baseball: Alexandria takes lessons from 0-2 trip
Andria Theatre holds auditions
Prairie Fire Children's Theatre looking for 'Tom Sawyer' actors
A wish come true for Kennedi Hay
A sweet setup to fight cancer
Garden concert features New Orleans sound
Engaged - Sharp and Kasper
Engaged - McCulloch and Bakker
Engaged - Steffel and Spangrud
Engaged - Friedrichs and Werk
Big smallmouth caught on Miltona
Equipment switch does the trick
A sunny day on Cowdry
Should have got a bigger net: Eight-year old lands 50-incher on Miltona
Mike Frisch: Froggin' for big bass
Victoria J. Dorn
Elnora Christine Beireis
Alvin E. Sibell
Steven Brian Ignowski
It's Our Turn: Road hogs, horn blasters and hell hounds
Commentary: Bait shop inspires 8-year-old
Letter: More taxis, more safe rides home
Letter: Franson doesn't back down on tough issues
Echo Press Editorial: New buffer law help for farmers
Millerville gets hands-on with butter
Tractors to parade down 36 mile memory lane
Douglas County 4-H campers
Cattlemen invite public to July 11 get-together
4-H announces K-3 summer camps
Schwan's announces acquisitions as frozen food company beefs up its pizza business
Aagard expands to pull all workers into one building
A new place for ink opens in Alexandria
Author Faith Sullivan to sign books in Alexandria
Knute Nelson partners with Centrex Rehab, adds memory care unit
hires and promotions
on the street
Update: 'Person of interest' in Long Prairie homicide now in custody
May 23, 2012
Growing Green - Tree planting tips
This unseasonably warm spring has encouraged avid home gardeners to get a jump start on their veggies. Potatoes, onions, peas, lettuce, spinach and radish have been planted, and it's just about warm enough to get the tomatoes, beans and peppers in the ground. (You want the soil temperature...
May 23, 2012 - 12:00am
November 28, 2011
Don't let pests come into your home this holiday season
Planning on buying a Christmas tree or holiday greens online this year? Be sure destructive pests aren't hitching a ride. As the popularity of online and mail order trees grows, state and federal officials are sending out a warning to consumers that these plant items are regulated...
November 28, 2011 - 3:03pm
August 15, 2011
MDA takes steps to protect state's walnut trees from deadly disease
Walnut wood is no longer allowed into Minnesota if imported from 11 different states known to have thousand cankers disease (TCD). A temporary exterior quarantine announced in February was made permanent last week by Minnesota Department of Agriculture (MDA) Commissioner Dave Frederickson....
August 15, 2011 - 8:49am
July 24, 2011
Ag department confirms emerald ash borer infestation in Shoreview, Minn.
ST. PAUL, Minn. - The Minnesota Department of Agriculture (MDA) confirmed an emerald ash borer infestation in an ash tree in a Shoreview residential neighborhood Friday. The infestation was discovered after the property owner noticed signs of a potential infestation and contacted...
July 24, 2011 - 11:51am
May 30, 2011
Arbor Day Foundation booklet identifies trees
The Arbor Day Foundation has a booklet that helps people identify trees in a simple, step-by-step process. The booklet, "What Tree Is That?," is available for a $5 donation to the nonprofit tree-planting organization. What Tree Is That? is a fun, easy-to-use tree identification guide...
May 30, 2011 - 3:43pm
April 22, 2011
Douglas Soil and Water reminds public to order trees
The Douglas Soil and Water Conservation District (SWCD) staff reminds the public to send in their tree order. There are still a good variety of trees to choose from, including small and large shrubs, deciduous trees, evergreen trees and potted evergreens. The SWCD staff is also available...
April 22, 2011 - 12:00am
February 26, 2011
Spring is in the air: Join Arbor Day Foundation in March and receive 10 trees
Celebrate the arrival of spring by planting trees, and the Arbor Day Foundation is making it easy for everyone to participate. Join the Arbor Day Foundation in March 2011 and receive 10 white pine trees. "White pines are such a versatile tree that adds beauty to your home and neighborhood...
February 26, 2011 - 4:24pm
Ag leaders implement quarantine to protect state's walnut trees from deadly disease
ST. PAUL, Minn. - Minnesota Agriculture Commissioner Dave Frederickson this week moved to protect the state's 6 million eastern black walnut trees and the state's walnut timber producers by issuing a temporary exterior quarantine restricting the import of walnut trees and certain...
February 26, 2011 - 2:04pm
February 2, 2011
City gets update on Highway 29 tree issue
The city of Alexandria, a group of concerned business owners and the Minnesota Department of Transportation are trying to come up with a plan about what to do with the trees along the Highway 29 South corridor. Business owners on the west side of the highway say the trees, when in...
February 2, 2011 - 12:00am
January 12, 2011
Conservation district can help with tree planting projects
Tree plantings can provide a number of different benefits from energy conservation, reducing snow delivery to roadways, providing wildlife habitat, aesthetics, farmstead protection from wind and snow, forestry production, reducing soil erosion by wind, stream bank protection and buffering...
January 12, 2011 - 12:00am
December 27, 2010
Be sure to dispose of Christmas trees properly
The National Fire Protection Association (NFPA) is offering reminders to keep folks fire-safe as they wind down the holiday season. Roughly one-third of home fires and home fire deaths occur in December, January and February, according to NFPA. Although Christmas tree fires are...
December 27, 2010 - 10:35am
December 15, 2010
Caring for your fresh-cut Christmas tree
One of my favorite holiday traditions is the selection of our fresh-cut Christmas tree. We often traipse through snow filled fields in bitterly cold weather to find that perfect tree. The end result is worth the frosted nose and rosy cheeks. Cut and placed in water immediately, our...
December 15, 2010 - 12:00am
November 21, 2010
Christmas tree supplies in tip-top shape this season
Minnesota has a fresh, fragrant crop of Christmas trees this year thanks to a great growing season. Many tree farms will open for business the day after Thanksgiving and growers say the selection will be fabulous. "This year, the trees are exceptionally moist and fragrant because...
November 21, 2010 - 12:20pm
October 6, 2010
All spruced up: Site of proposed high school gets nearly 200 trees
The site of the proposed new high school was spruced up last week with the addition of nearly 200 trees. Last Wednesday, about 30 volunteers...
October 6, 2010 - 12:00am
October 1, 2010
Storm-toppled trees keep REA crews busy
Pouring rain, howling wind and crashing thunder are not the only products of thunder storms. Power outages are another common side effect of...
October 1, 2010 - 12:00am


New Zealand International Early Music Festival
By NZIEMF | Posted: Friday February 22, 2019
New Zealand International Early Music Festival (NZIEMF) 22 February to 10 March 2019.
We have held three successful Early Music Festivals in Dunedin during 2014, 2015 and 2016.
Calling all Early Music Enthusiasts Worldwide!! We are now seeking funding of $100,000 for a NZIEMF during 2019. Please contact firstname.lastname@example.org with any amount you can pledge.


text:
Get the complete project »
- The Complete Research Material is averagely 52 pages long and it is in Ms Word Format, it has 1-5 Chapters.
- Major Attributes are Abstract, All Chapters, Figures, Appendix, References.
- Study Level: MTech, MSc or PhD.
- Full Access Fee: ₦8,000
The Studyassessed the adoption of recommended management practices for quail production in two selected Local Government Areas (Kano municipal and Gwale) of Kano state. Multi-stage samplingtechnique wasused to select 120respondentsfrom two districts each from the two local government areas.. BothDescriptive and Inferential statistics were used to analyze the data.The result of the analysis revealed that the mean age of the respondents was39 years, minimum primary level of education was found to be 37%, awareness and quail farming experiencewere significant in the adoption of recommended practicesforquail production in the study areas(72% and 75% respectively). The average rate of adoption for the recommended management practices for quail production in the study areas was 65%. The mean income of the respondents before and after adoption of recommended practices was found to be 2.18(N30, 000) and 3.37(N45, 000) respectively. The study therefore shows that adoption of recommended management practices for quail production has a significant impact on the farmer‘s income, production output and standard of living in the study areas.
1.1 Background to the Study
Quail (Cortunixcortunix Japonica) is a native of Asia and Europe. It has been farmed
since ancient times, especially in the Far East. They reproduce rapidly and their rate
of egg production is remarkable. They are also robust, disease resistant, and easy to
keep, requiring only simple cages and equipment and little space. Yet they are not
well known around the world and deserve wider testing. Quail are so precocious that
they can lay eggs when hardly more than 5 weeks old. It is said that about 20 of them
are sufficient to keep an average family in eggs year-round. Quail eggs are very
popular in Japan, where they are packed in thin plastic cases and sold fresh in many
food stores. They are also boiled, shelled, and either canned or boxed like chicken
eggs (Vali, 2008).
Commercial quail farming is becoming more popular and is being increasingly
promoted in number of Asian
You either get what you want or your money back. T&C Apply
You can find more project topics easily, just search
SIMILAR AGRICULTURAL ECONOMICS FINAL YEAR PROJECT RESEARCH TOPICS
» CHAPTER I 1.0 INTRODUCTION Agriculture, no doubt plays a significant role in the economic development in Nigeria, agriculture, whether subsistence pri...Continue Reading »
2. AN EVALUATION OF NATIONAL FUNDING AND INVESTMENT IN THE AGRICULTURAL SECTOR OF NIGERIA (1970-2008)» ABSTRACT The research study evaluated National funding and investment in the agricultural sector of Nigeria (1970 2008). Information was gathered from...Continue Reading »
» CHAPTER ONE INTRODUCTION 1.1 BACKGROUND TO THE STUDY The country food situation during the last and present decades becomes as critical as to constitu...Continue Reading »
» ABSTRACTCheese, a popular food across many cultures has been subject of many studies. It’s of many variants, providing huge nutritional value to hum...Continue Reading »
» CHAPTER ONE INTRODUCTION BACKGROUND TO THE CASE STUDY The advent of computer technology has boost production of both domestic and industrial activitie...Continue Reading »
» CHAPETR ONE INTRODUCTION 1.1 BACKGROUND OF THE STUDY It is said that to mange on organization is to manage its future. How can the future be managemen...Continue Reading »
» ABSTRACT climate change. Agricultural insurance is seen as one of the best strategies to address farm risks and encourage the affected farmers to get ...Continue Reading »
» BSTRACTComparisons were made between 24 Ethiopian rams and 24 Galla goats for voluntary intake, feed selectivity and growth performance when offered t...Continue Reading »
9. LIVELIHOOD DIVERSIFICATION UTILIZED BY RURAL DWELLERS IN NSIT IBOM LOCAL GOVERNMENT AREA OF AKWA IBOM STATE» ABSTRACT Comparative Analysis compares two or more languages with common descent from a shared ancestor feature by feature, in order to extrapolate ba...Continue Reading »
» CHAPTER ONE 1.0 INTRODUCTION 1.1 BACKGROUND OF STUDY The human population growth in developed countries is stabilizing while that of developing countr...Continue Reading »


text:
OCEAN TOWNSHIP – Senator Vin Gopal and Assembly Members Eric Houghtaling and Joann Downey are calling for Governor Murphy to permit curbside pickup at New Jersey public libraries in order to promote public education and expand access to resources and educational materials during the COVID-19 pandemic.
“Contactless curbside pickup is a safe way for our residents to get access to books, programs, and titles that can educate, entertain, or engage,” said Gopal (D-Long Branch). ”At a time when so many activities are off-limits due to the risk they pose to public health, it’s important to find places where we can meet our community’s essential needs by safely reopening.”
“Some New Jersey libraries, such as the Neptune Township Public Library, have already done an outstanding job of showing how these programs might be implemented,” said Houghtaling (D-Neptune). “Instead of hindering access to education, let’s open the door for libraries to continue empowering their readers, both virtually and in paperback.”
“A book can create a path to new skills, develop a reader’s mind, or expand a child’s imagination,” said Downey (D-Freehold). “Libraries do so much to serve the needs of their communities, and I have full confidence that our passionate librarians will be able to adapt to the challenges of today’s pandemic.”

domain: politics


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Day Dream – the second 10×10 in The Forty Mini Collection has just been released!
Posted by katie berggren on
I’m on the ball today, and feeling much better, thank you
Day Dream is now released! The 2nd in The Forty Mini Collection – see Day Dream, and last week’s release: Wist, here .
I’ve also just listed five new glass art charms in the Online Etsy shop – after this weekend’s bazaar in Portland I’ll be listing some more.
Related Images:[See image gallery at www.paintingmotherhood.com]
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- Tags: motherhood art

domain: hobby


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Exploring the most Picturesque & Historic parts of England
Below is a comprehensive list of hotels in Roman Baths. Use the sort links to refine your search or to find featured accommodation in Roman Baths. Many of our hotels in Roman Baths feature no booking fees and best price guarantee when booked through the Pictures of England website.
To list your hotel in Roman Baths, click here.
Other popular nearby attractions for Hotels
Longleat House & Safari Park hotels Blacknore Lighthouse hotels Clevedon Court hotels Oldbury Court Estate, Bristol hotels Horton Court hotels Biss Meadows Country Park hotels Caldicot Castle hotels Beckford's Tower & Museum hotels Buildings of Bath Museum hotels Keynes Country Park hotels

domain: history


text:
Menin-MLL inhibitor blocks progression of middle ear cholesteatoma in vivo
Tomomi Yamamoto-Fukuda a,*, Naotaro Akiyama b, Norifumi Tatsumi c, Masataka Okabe c,
Hiromi Kojima a
a Department of Otorhinolaryngology, Jikei University School of Medicine, Tokyo, Japan b Department of Otorhinolaryngology, Toho University School of Medicine, Tokyo, Japan c Department of Anatomy, Jikei University School of Medicine, Tokyo, Japan
A R T I C L E I N F O
Keywords: Cholesteatoma Menin-MLL inhibitor KGF
Level of evidence: N/A
A B S T R A C T
Objective: Cholesteatoma is an epithelial lesion that expands into the middle ear, resulting in bone destruction. The acceleration of the proliferative activity of epithelial stem/progenitor cells is involved in the pathogenesis of cholesteatoma. Recently, the use of a menin-mixed lineage leukemia 1 (MLL1) inhibitor, MI503, in experiments has resulted in inhibition of the growth of tumors under histone modification. In this study, we investigated the effects of the menin-MLL inhibitor against cholesteatoma growth in an in vivo model.
Methods: We first correlated the expression level of histone H3 trimethylation at lysine 4 (H3K4me3) among cholesteatoma cases, chronic otitis media cases and normal skin tissues. Based on the role of keratinocyte growth factor (KGF) in the development of cholesteatoma, KGF-expression vector was transfected into the ear and we analyzed the expression level of H3K4me3. After cholesteatoma was induced, MI503 was administered daily into the ear for 14 days.
Results: We detected the highest labeling index of H3K4me3 in the cholesteatoma specimens. After KGF- expression vector transfection in the mouse ear, a high expression level of H3K4me3 was observed in the epithelial layers. The use of MI503 reduced cholesteatoma in the in vivo model and decreased the proliferation of epithelial stem/progenitor cells in a dose-dependent manner.
Conclusion: We demonstrated that inhibition of the menin-MLL interaction may be a potentially useful strategy in the conservative treatment of cholesteatoma.
Cholesteatoma is a gradually expanding, destructive epithelial lesion within the middle ear, which leads to extensive tissue destruction in the temporal bone [1,2]. Recently, we demonstrated that the disruption of homeostasis of stem/progenitor cells in the epithelium is an important pathogenesis of cholesteatoma .
Histone modification, especially histone H3 trimethylation at lysine 4 (H3K4me3), has been reported to regulate stem cell self-renewal and differentiation  and the frequent mutation of H3K4me3 promotes developmental disease and tumor initiation . Menin is a highly spe- cific binding partner of mixed-lineage leukemia 1 (MLL1), a histone methyltransferase that catalyzes H3K4me3  and is required for the recruitment of the MLL1 complex to the target genes . Recent studies demonstrate that the menin-MLL inhibitor MI-503 shows antitumor activity in in vitro and in vivo models [8,9].
The primary purpose of the present study was to analyze the expression level of H3K4me3 in human cholesteatoma specimens. The secondary objective was to investigate the effects of MI503 during cholesteatoma formation in vivo. The results suggest the promising po- tential of treatments designed to target histone modification.
⦁ Materials and methods
⦁ Human cholesteatoma
We examined the middle-ear tissues of 20 patients (three women, 17 men) with cholesteatoma (10 cases) and chronic otitis media (COM) (10 cases). In the ears of the study subjects with cholesteatoma, a small piece of normal skin tissue was also harvested as a control. This study protocol was approved by the Human Ethics Review Committee of the Jikei
* Corresponding author. 3-25-8, Nishishinbashi, Tokyo, Minato-ku, 105-8461, Japan.
E-mail address: [email protected] (T. Yamamoto-Fukuda).
Received 5 July 2020; Received in revised form 11 November 2020; Accepted 1 December 2020
Available online 3 December 2020
0165-5876/© 2020 Elsevier B.V. All rights reserved.
University School of Medicine (No. 27–344 8229).
⦁ Immunohistochemical analysis of H3K4me3
For the detection of H3K4me3, an enzyme immunohistochemistry was performed as described previously [3,14]. Briefly, after the antigen retrieval was performed by autoclave in Histo VT one (Dako), the slides were incubated overnight with anti-H3K4me3 antibody (rabbit mono- clonal, Cell Signaling, #9751; 1:200). Dako REAL EnVision Detection System kits were used as secondary antibodies. After reaction with the HRP-conjugated secondary antibodies, the sites of HRP were visualized with DAB and H2O2. For a negative control, normal mouse or rabbit IgG (1:100) was used instead of the first antibody.
⦁ In vivo experiments
The experiments were conducted in BALB/c mice (6 weeks, 30–35 g) with a normal tympanic membrane (TM). Animal care and experimental
procedures were performed in accordance with the Guidelines for Ani- mal Experimentation of Jikei University with approval guidelines (No. 2015-139C4).
⦁ Electroporatically transfection of hKGF-expression vector to the mice ears
Flag-h keratinocyte growth factor (KGF) DNA plasmid driven by a CMV14 promoter (0.5 μg/ml) (KGF-expression vector) were transfected
five times every fourth day into the epithelial lesion of the mouse ear canals using a Nepa21 Electroporator (Nepa Gene Co., Chiba, Japan) in a prepared experimental cholesteatoma model in vivo according to the protocol of a previous paper . As a control, a null-plasmid driven by
a CMV14 promoter (0.5 μg/ml) (empty vector) was transfected in the
other ears of the mice .
⦁ Administration of menin-MLL inhibitor in vivo
KGF-expression vector was transfected five times every fourth day into the epithelial lesion of the ear canal of a prepared of experimental cholesteatoma model in vivo . After confirming KGF
expression-vector-induced cholesteatomas in the mice via an otoendo- scopic examination, as had been done previously , the 30 μl menin-MLL inhibitor (500 μM, 50 μM or 5 μM MI503 in 2% dimethyl
sulfoxide (DMSO) in PBS or 2% DMSO in PBS, n 4 each) (Selleck Chemicals, Houston, TX) was administrated topically via the ear canal for 14 consecutive days  and an otoendoscopic examination was performed .
⦁ Tissue preparation
To analyze the effects of KGF-expression vector against the expres- sion level of H3K4me3 the mice were euthanized using an intraperito- neal injection of 200 mg/kg pentobarbital and their ear tissues were
removed at Days 1, 4 and 7 (n 3, each) after vector transfection, fixed with 4% PFA in a phosphate-buffered saline (PBS) at 4 ◦C overnight and embedded in paraffin in a standard manner. For the histopathological
analysis of MI503-effects against cholesteatoma formation, the mice were euthanized using an intraperitoneal injection of 200 mg/kg pentobarbital and their temporal bones were removed at 24 h after the
final administration of MI503. The temporal bones were fixed with 4% PFA in PBS at 4 ◦C overnight, decalcified by 10% ethyl- enediaminetetraacetic acid at 4 ◦C for seven days and paraffin sections
of the temporal bones were prepared as described previously [12,13].
⦁ Otoendoscopic examination
In the present study, cholesteatoma formation was evaluated by otoendoscopy. At the seventh day after the final KGF-expression vector transfection and at one day after the MI503 administration, the mice at each time-point underwent otoendoscopic examination with a rigid rod
0◦ otoendoscope (AVS Co., Tokyo, Japan).
⦁ Pathological and immunohistochemical analysis
Hematoxylin and eosin (H&E) staining was performed to analyze the pathological findings of the mouse ears. For the detection of KGF, H3K4me3 and proliferating cell nuclear antigen (PCNA), an enzyme immunohistochemistry was performed as described previously [3,14]. Antigen retrieval was performed by autoclave in Histo VT one for H3K4me3. The slides were incubated overnight with first antibodies (anti-KGF [rabbit polyclonal, Thermo Fisher Scientific, PA5-49715; 1:50]; anti-H3K4me3 [rabbit monoclonal, Cell Signaling, #9751; 1:200]; anti- PCNA [mouse monoclonal, Dako, AB2160651; 1:200]). Dako REAL EnVision Detection System kits were used as secondary antibodies. After reaction with the HRP-conjugated secondary anti- bodies, the sites of HRP were visualized with DAB and H2O2 or in the presence of nickel and cobalt ions. For a negative control, normal mouse or rabbit IgG (1:100) was used instead of the first antibodies.
⦁ Detection of stem/progenitor cells in the cholesteatoma specimens after MI503 administration in vivo
To analyze the effects of MI503 against stem/progenitor cells in the cholesteatoma specimens, we performed an enzyme immunohisto- chemistry by using anti-p63 antibody (a stem/progenitor cell marker). After the antigen retrieval was performed by autoclave in Histo VT one, the slides were incubated overnight with anti-p63 antibody (mouse monoclonal, Abcam, ab735; 1:50). Dako REAL EnVision Detection System kits were used as secondary antibodies. After reaction with the HRP-conjugated secondary antibodies, the sites of HRP were visualized with DAB and H2O2. For a negative control, normal mouse IgG (1:100) was used instead of the first antibodies.
⦁ Statistical analysis
The quantitative analysis was performed as described previously and the labeling index (LI) was expressed as mean SD . Differences
between the groups were examined for statistical significance using the unpaired Student’s t-test and the one-way analysis of variance test fol- lowed by the Tukey’s post hoc tests for normally distributed data (JMP
version 13; SAS Institute Japan). A p value of less than 0.05 denoted a statistically significant difference.
⦁ Analysis of H3K4me3 expression in human cholesteatoma tissues
In the normal skin tissues, a few H3K4me3-positive cells were detected in the basal and spinous layers of the epithelium (Fig. 1A). In the COM tissues, some H3K4me3-positive cells were detected in the basal and spinous layers of the epithelium (Fig. 1A). The localization of H3K4me3-positive cells of the normal skin tissues and COM tissues was the same (Fig. 1A). On the other hand, in the cholesteatoma tissues, intense staining for H3K4me3 was predominantly found in almost all of the cells of the epithelium, and a few H3K4me3-positive cells were found in the subepithelium (Fig. 1A). In addition, when the sections were reacted with preimmune normal rabbit serum instead of the first anti- body, no staining was found (data not shown).
In the human tissue specimens, the number of H3K4me3-positive epithelial cells significantly increased in the cholesteatoma group (88.8 ± 4.0%) as compared to the COM group (vs 50.5 ± 18.1%, p < 0.0001) and normal skin control (vs 16.5 ± 5.0%, p < 0.0001) (Fig. 1B). ⦁ Analysis of H3K4me3 expression in an vivo model KGF-expression vector was successfully transfected and KGF protein was expressed in the many stromal cells and epithelial cells at Days 1 and 4 after vector transfection, the same as the results described previ- ously (Fig. 1C) . H3K4me3 was detected in many basal cells and some upper basal cells in the epithelium at Days 1 and 7 after Fig. 1. Analysis of H3K4me3 expression in human tissues and the in vivo model. (A) Immunohistochemical analysis of H3K4me3 in cholesteatoma (Chole), chronic otitis media (COM) and normal skin (Skin). H3K4me3-positive cells were mainly detected in the epithelium. Scale bar: 50 μm; arrows: positive cells. (B) Box plot showing the H3K4me3 LI in each group of humans (n = 10 each). Data are mean ± SD, ****: p < 0.0001. (C) Immunohistochemical analysis of KGF in KGF- expression vector-transfected ear (KGF) (Day 1 and Day 4) and vector alone-transfected ear (Empty) (Day 1). Many KGF-positive cells were shown in the epithe- lium and subepithelial regions. (D) Immunohistochemical analysis of H3K4me3 in KGF-expression vector-transfected ear (KGF) (upper panels, Day 1, Day 4 and Day 7) and vector alone-transfected ear (Empty) (lower panel, Day 1). Single transfection of KGF-expression vector-induced H3K4me3 expression in the epithelial cells. E: epithelium; S: subepithelium; dashed line: basement membrane; scale bar: 20 μm; arrows: positive cells. (D) Box plot showing the H3K4me3 LI in each group of mice (n = 3 each). Data are mean ± SD, *: p < 0.05, **: p < 0.01, ***: p < 0.001. KGF-expression vector transfection (Fig. 1D). We also noted many cells in the upper layer were also positive for H3K4me3 in the KGF-expression vector-transfected ears at Day 4 (Fig. 1D). In contrast, a few H3K4me3-positive cells were detected in the basal and upper basal layers of the epithelium in the vector alone-transfected (empty vector) ears at any day (Fig. 1D). In addition, when the sections were reacted with preimmune normal rabbit serum instead of the first antibody, no staining was found (data not shown). ± ± ± In the vivo model specimens, the number of H3K4me3-positive epithelial cells significantly increased in the KGF-transfected group at Day 1 (37.0 2.7%), Day 4 (33.9 3.2%) and Day 7 (35.4 2.4%) as ± compared to the empty group at Day 1 (vs 20.7 4.0%, p < 0.01), Day 4 ± ± (vs 14.8 1.6%, p < 0.001), and Day 7 (vs 19.1 6.2%, p < 0.05) (Fig. 1E). ⦁ Effects of MI503 against cholesteatoma tissues in vivo According to the results of the otoendoscopic analysis, choles- teatoma formations were shown in the TM of all of the KGF-vector transfected ears in vivo (Table 1, Fig. 2A, upper column Day 0), the same results as in the previous report . To clarify the efficacy of MI503 in suppressing cholesteatoma formation in vivo, we analyzed the ears by otoendoscopy and histology, as had been done previously . At one day after the final treatment with 5 μM MI503 in the in vivo model, cholesteatoma was reduced in two of four ears (Table 1, Fig. 2A, lower column Day 14). A histological examination indicated a slightly thickened epithelial layer of the TM with debris in the tissue sections from the KGF expression-vector induced cholesteatoma treated with 5 μM MI503 (Fig. 2B). After the treatment of cholesteatoma with 50 μM Table 1 Analysis of the pathological state of middle ears under administration of MI503 in in vivo model. Cholesteatoma (+) Cholesteatoma (—) 5 μM MI503 (n = 4) 2 2 50 μM MI503 (n = 4) 0 4 PBS (n = 4) 4 0 Fig. 2. Effects of MI503 against cholesteatoma tissues in vivo. (A) Otoendoscopic view of the tympanic membrane (TM) with administration of a menin-MLL inhibitor (500 μM, 50 μM or 5 μM MI503) or control (PBS) at Days 0 and 14. Before administration, cholesteatoma (Chole) was detected in all of the ears. After administration of 5 μM MI503 in the ear, debris was detected on the TM. After administration of 50 μM and 500 μM MI503 in the ear, cholesteatoma was not detected. (B) H&E staining of the TM with administration of a menin-MLL inhibitor (500 μM, 50 μM or 5 μM MI503) or control (PBS) at Day 14. Scale bar: 100 μm. MI503, cholesteatoma was not detected in any of the ears (Table 1, Fig. 2A, lower column Day 14). In fact, a histological examination indicated almost a normal structure of the TM and middle ear in the tissue sections from the KGF expression-vector induced cholesteatoma treated with 50 μM MI503 (Fig. 2B). The dosage of 500 μM MI503 also reduced cholesteatoma, but did induce crust and a necrotic reaction in the TM (Fig. 2A, lower column Day 14, B). As a control of this exami- nation, PBS was administered topically instead of MI503, and four our of four cholesteatomas were detected (Table 1, Fig. 2A, lower column Day 14). The topical administration of MI503 resulted in a significant sup- pression of cholesteatoma formation in a dose-dependent manner (cholesteatoma+/total number: 2/4 [5 μM], 0/4 [50 μM], 0/2 [500 μM], 4/4 [PBS]) (Table 1). ⦁ The effects of MI503 against trimethylation of histone H3K4, proliferative activity of epithelial cells and the number of stem/progenitor cells in cholesteatoma in vivo To analyze the effects of MI503 against the expression of H3K4me3 in vivo, we indicated the results of our immunohistochemical analysis (Fig. 3A, left column). Many H3K4me3-positive cells were indicated in the epithelial layer of the TM treated with PBS (Fig. 3A, left column). On the other hand, few H3K4me3-positive cells were detected in the epithelial layer of the TM treated with MI503 (Fig. 3A, left column). To evaluate the effects of MI503 against the proliferative activity of epithelial cells and the number of epithelial stem/progenitor cells, we analyzed the expression of PCNA (proliferative cell marker) and p63 (stem/progenitor cell marker) by immunohistochemistry. As shown in Fig. 3A, a large number of PCNA-positive cells and p63-positive cells were detected in the epithelial layer of the TM treated with PBS (middle and right column). On the other hand, a small number of PCNA-positive cells were detected in the basal layers of the epithelium of the TM treated with MI503 (Fig. 3A, middle column). The localization of p63- positive cells was almost the same as that of the PCNA-positive cells, and a few p63-positive cells were detected in the basal layers of the epithelium of the TM treated with MI503 (Fig. 3A, right column). The number of H3K4me3-positive epithelial cells significantly decreased after MI503 administration (50 μM; 1.2 ± 0.5%, 5 μM; 4.5 ± 2.5%, PBS; ± ± ± 16.6 ± 2.9%, p < 0.0001, Fig. 3B). PCNA LI was significantly lower in the MI503 group than in the control group (50 μM; 1.5 0.6%, 5 μM; 7.5 3.1%, PBS; 19.3 5.1%, p < 0.0001, Fig. 3B). The number of stem/progenitor cells (p63 LI) also decreased after MI503 Fig. 3. The effects of MI503 against stem/progenitor cells in cholesteatoma in vivo. (A) Immunohistochemical analysis of H3K4me3, PCNA and p63 in the in vivo model with a menin-MLL inhibitor (50 μM or 5 μM MI503) or PBS. Arrows, positive cells. (B) Box plot showing the H3K4me3 LI in each group (n = 4 each). ****, p < 0.0001. ± ± ± administration (50 μM; 0.8 0.3%, 5 μM; 2.2 0.8%, PBS; 9.6 1.7%, p < 0.0001, Fig. 3B). ⦁ Discussion In this study, we showed the presence of a high level of H3K4me3 in human cholesteatoma for the first time (Fig. 1A and B). Our study demonstrated the possibility that the pathogenesis of cholesteatoma might be correlated with epigenetic dysregulation. Not only our results, but a recent study also demonstrated that the pathogenesis of cholesteatoma is correlated with epigenetic dysregulation under the role of circular RNAs, which supports our results . Recently, it has been shown that H3K4me3 is commonly associated with the activation of transcription of nearby genes, and H3K4me3 ChIP-seq is usually examined to identify active gene promoters . A previous study revealed the pathological significance of a high level of H3K4me3 correlation in inflammatory disease [17–19]. Also, increased cytokine levels of the genes, including interleukin (IL)-6 and tumor necrosis factor (TNF)-α, were detected in rheumatoid arthritis patients, which in turn were associated with increased H3K4me3 in the promoter region . Cholesteatoma is also a chronic inflammatory disease and a high expression level of IL-6 and TNF-α was observed in the previous studies [20,21]. Hence, these data are consistent with our results, although further studies are necessary to evaluate the genes that are transcripted related to H3K4me3. Fibroblast growth factor (FGF) signaling is known to control changes in the epigenetic signature and the level of histone modifications, including methylation of histone H3K4 in mouse embryonic stem cells during differentiation . In our previous study, we showed that stromal KGF/FGF 7 played an important role in human cholesteatoma formation and recurrence, as characterized by the hyper-proliferation of epithelial cells . In light of these facts, we postulated that H3K4me3 induction under KGF expression might play an important role in the pathogenesis of cholesteatoma. In fact, in this study we showed a significantly increased number of H3K4me3-positive cells in the KGF-expressed vector transfected ear tissues (Fig. 1C and D) and KGF-induced cholesteatoma tissues in vivo (Fig. 3). We next hypothesized that menin-MLL inhibitor, MI-503, could reduce cholesteatoma under suppression of H3K4me3. To investigate this hypothesis, we administrated MI-503 in the KGF-induced choles- teatoma tissues in vivo. Our results showed that the formed choles- teatoma decreased PCNA and p63-positive stem/progenitor cells in a dose-dependent manner with MI503, which reduced cholesteatoma (Fig. 3). Recently, Menin-MLL inhibitor has been investigated as a therapeutic target and several menin-MLL inhibitors could reduce the expression of the target genes [8,9]. Moreover, Ding and colleagues  indicated that the menin-MLL inhibitor specifically inhibited prolifera- tion of HCC cells, as same as with our results. These reports suggest that MI503 may decrease the proliferative activity of cells by suppressing
some genes’ expression as direct targets of H3K4me3. In our previous
study, we indicated that an increased number of p63-positive stem/- progenitor cells were collated in the pathogenesis of cholesteatoma and the over-expressed KGF-induced proliferative activity of p63-positive stem/progenitor cells in mouse ears in vivo . In this study, the num- ber of p63-positive stem/progenitor cells was shown to have decreased under MI503 administration (Fig. 3). These results suggest that MI503 may be a negative regulator of p63 expression under the inhibition of gene transcription associated with H3K4me3. In the recent study, transcription factor p63 was shown to bind to H3K4me3 and induce gene transcription associated with p63 , which supports our hy- pothesis. In other words, H3K4me3 may control the proliferative ac- tivity of p63-positive stem/progenitor cells during cholesteatoma formation.
In conclusion, to the best of our knowledge this is the first study to show the higher expression level of H3K4me3 in cholesteatoma speci- mens. KGF may accelerate the trimethylation of H3K4 and induce pro- liferative activity of epithelial cells in the TM, resulting in epithelial hyperplasia and stratification with debris, as in cholesteatoma forma- tion. Interestingly, the number of p63-positive cells (stem/progenitor cells) was shown to have significantly decreased under the administra- tion of menin-MLL inhibitor, an inhibitor of the trimethylation of H3K4. Finally, the cholesteatoma formations were shown to have disappeared in the experiment. Controlling the modification of histone methylation could be a potential therapeutic target for the conservative treatment of cholesteatoma in the future.
This study was supported by JSPS KAKENHI Grant Number JP 19K09857 (to T. Yamamoto-fukuda).
Declaration of competing interest
The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this paper.
This study was supported by JSPS KAKENHI Grant Number JP 19K09857 (to T. Yamamoto-fukuda). KGF-expression vector was kindly provided by Matsumoto K. We thank Takahashi M, Yamamoto K and Yamamoto Y for the harvesting of the human tissues.
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domain: medical


text:
There’s a steady stream of customers coming through the doors of Rahiba Rahimi’s fashion studio. The 25-year-old’s bold, intricate designs are fitted on mannequins and hung on rails around her showroom in Kabul.
Rahimi is the lead designer and co-proprietor of Laman, a clothing label she helped build in the Afghan capital five years ago.
Her customers are mostly Afghan women and men, together with a few foreign nationals who work in Kabul’s aid-driven economy.
“A lot of times we collaborate and co-design with customers because Afghans are very good at fashion. Even now, if you see on the street, you will see how Afghans express their creativity in colours and styles. We are here to help with the quality of that expression, with design, details, fabric,” she says, walking around the multistorey building that doubles as a workshop and studio.
“I haven’t studied fashion designing, this came about based on my love for colours and patterns and, in a broader sense, my love for the culture and heritage.”
Her designs are a world away from what might be considered, outside the wartorn country at least, the norms of Afghan fashion. Clothing styles in the country have traditionally been influenced by social norms formed over decades of conflict, violence and extremism. Yet that hasn’t deterred a younger generation of Afghans from pushing the boundaries, while also attempting to revive traditional handicraft and art.
“Fashion for me represents a strong statement in a conservative society. It is an expression of myself as a woman and as an Afghan youth. I should have the right to wear the colours I want to wear. It is a sign of freedom,” Rahimi explains.
Rahimi talks about the new Laman designs, many of which featured in a fashion show in Milan in June.
The clothes aren’t entirely traditional, but a fusion of modern styles using lighter fabric. “We are making Afghan fashion global while also keeping certain things and values the same,” she says, picking up shirts with vintage Baluchi-Nuristani hand embroidery and coats edged with embroidered leather. There are brightly coloured long dresses adorned with intricate beadwork and a very specific embroidery design with sharp edges, like a dagger.
Despite the immense diversity, colour and variety, Afghan fashion is not well known internationally. “The image you see from far is one with women in burqas and chader [a long black outer garment]. But go to an Afghan wedding and that image will be broken,” Rahimi says.
However, she has noticed a significant shift in how Afghan fashion is perceived since she first started the company. “I do get a lot of surprised reactions to my designs when I am abroad, but that is changing. Even in international fashion, there is so much diversity – and Afghanistan’s fashion is finding its place,” says Rahimi, who employees two dozen Afghans, including female designers, tailors and sales staff.
Rahimi, who is also completing her undergraduate degree in business management, admits that despite their growth, profit margins remain low. “Finances are not always [a] lot, but we have so far managed to break even. It is still a small to medium business, with investment from my family, particularly my father, who believes in me,” she says, adding that her female employees are central to her drive to keep going. “It is not just about the fashion, but also about creating jobs and empowering other women. They have grown so much with the brand; some of them are breadwinners for their families,” she says.
Hasina Aimaq, 26, another designer breaking taboos and stereotypes, also showcased her work at the Milan show, held in the historical Salone Dei Tessuti.
Aimaq’s bold designs attracted both acclaim and criticism. Her signature piece, a hand-embroidered blue dress inspired by the traditional blue burqa worn by women in Afghanistan, was much discussed in the country, and stirred the ire of many conservative Afghans.
Sitting in her small studio in the basement of a house in the west of Kabul, a much more basic operation than Rahimi’s showroom, she shares the motivation behind her most controversial, yet also most commissioned design from the Milan set.
“My aim was to share a positive image of Afghanistan and specifically showcase the talents of Afghan women through these designs,” she says, sitting next to a large working table where samples from the Milan fashion show are laid out. “Everyone recognises the blue burqa as Afghan clothing, so I thought: ‘Let’s make something else out of this and show them what Afghan women are capable of.’”
Dismissing the critics, Aimaq says she was encouraged the support she got, especially from women in the rural north: “I also received some positive comments, because many Afghan women acknowledge that the chader is not really part of our culture. It’s just a fabric and we can make anything out of that.”
All of Aimaq’s work is inspired by traditional Afghan handicrafts and designs, and uses locally sourced materials, even precious stones, for which the country is known. Her designs, like Rahimi’s, are a fusion of the traditional and contemporary. Another dress displayed in her studio has separate cuffs, reminiscent of Wonder Woman, with detailed embroidery. “Without saying anything, she [a woman wearing the dress] says she’s so powerful,” says Aimaq. “Each piece in this collection has a message and a story behind it.”
She started designing in 2011, inspired by her mother, a widow who secretly worked as a tailor during the years of the Taliban regime, defying its prohibition on female workers. “She taught herself to sew and stitch, got better with practise and set up her small, discreet business,” Aimaq says. “My mother would stitch clothes for the neighbours and slowly started to move her products to the local markets, despite risks of being punished by the Taliban. Since I was young, I have known that she wanted to turn this into a proper business and together we did it,” she adds proudly.
Aimaq’s business provides work for more than 100 women across the Kabul and Afghan provinces, who work from home and help produce wholesale quantities of regular clothing, as well as specially designed dresses and boutique garments.
“[At the] beginning of every month, we collect all the raw material and drop it off with our home producers. They do the embroidery and sewing as needed. At the end of the month or around deadlines, we collect their work and bring it here, where two or three tailors finish the production,” she says. The system offers many women who are unable to leave home due to social restrictions the opportunity of financial independence. Each can earn 20,000 and 35,000 Afghanis (£200-£348), a substantial contribution to an Afghan household.
Being women pioneers in their field comes at a cost for Aimaq and Rahimi. Both have received threats and intimidation on- and offline. Aimaq was forced to move her studio because of the threats. But they remain determined. “We survived worse times during the Taliban regime. We are more empowered now than we were back then,” Aimaq says.

domain: artistic


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Here's a gaming mouse that will make you feel like you're driving a light cycle in the movie Tron: Legacy.
Not only does this ambidextrous pointing device from Razer look like some sort of implement from the far-off future; it sounds like one, too.
Tron: Legacy was the number-one movie in the U.S. last weekend, so this mouse is certain to attract the attention of fans. But will it measure up for gamers? Let's get our hands on one.
Besides the fact that this is a wired mouse — a controversial attribute for a gaming mouse among aficionados — the one spec gamers will care most about is this mouse's fine 5600 DPI 3.5G sensor. Its sensitivity is excellent, and its two extra buttons on each side are programmable for any function. So yes, its specs are gameworthy, though not groundbreaking.
It's a shame that this $100 mouse has such a plastic feel to it. Its all-plastic construction feels just a little too light for my taste, and its left and right click sounds are slightly loud. Those quibbles aside, the mouse feels balanced in a medium-sized hand, and I got accustomed to it quickly.
Its claim to fame is its Disneyesque lighting and sound effects. I especially like the way the rubberized mouse wheel resembles the wheels on those Tron light cycles. And that lighting stays on all the time, except for a light blue taillight that glows only when the mouse's laser sensor is engaged.
When you first plug it in, it makes whooshing sound effects and its lights flash, an odd routine that startled me a couple of times. Check out the video below to see and hear with that's like. It's fun to show that to your friends when you first get it, but thankfully, the noises can be turned off in the software controls as soon as the novelty has worn off.
The Tron Gaming Mouse is available for $100, but if you want to get the full effect, you can spend $30 more to get a set that includes a bioluminescent mat. Its most unusual characteristic is the way it displays a blue glow for a few moments wherever the mouse's laser has shown onto it. Besides its snazzy landing-pad appearance, it has just the right amount of friction to perform as a suitable complement to the Tron mouse.
Sure, this is a Disney-licensed product that's mostly a gimmick, but it's a fun gimmick, and it looks way cool on the desktop. You might not want to whip this baby out at the office; but for your gaming center, it could be a suitable centerpiece for Tron fans.


Former prime minister Syed Yousaf Raza Gillani Monday filed a petition in the Islamabad High Court (IHC) challenging the rejection of seven votes in the election for Senate chairman’s slot. Senator Gilani moved the petition through his counsel Farooq H Naek requesting the high court to declare the result of the election of Senate chairman held on March 12 ‘illegal, unlawful and void’. In the petition, Gilani pleaded that seven votes cast in his favour were rejected by the RO during the election for the Senate chairman, adding that the RO’s order to declare the votes invalid should be overturned. Gilani alleged in his petition that the government had attempted to influence the result of the election to win Senate top slot ‘by hook or by crook’. “During the process of counting of votes, the presiding officer (Syed Muzaffar Hussain Shah) arbitrarily rejected seven of the votes Cast in the petitioner’s favour on the ground that stamp was affixed on the name of the petitioner despite protest of the petitioner’s polling agent Senator Farooq H Naek that the stamp had been fixed within the box containing the name of the petitioner,” he contended. “The votes rejected by the presiding officer clearly evince the voter’s intent to vote for the petitioner and no one else,” the petitioner argued. Moreover, he added, these are in compliance with the notice affixed near the polling booth as well as the understanding conveyed by the Senate secretary.


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I have a lovely Arabian mare who I would like to breed. She is very well
Booty as I call her is a amazing animal. I really think this girl was
About Arkansas City,KS Present-day Arkansas City sits on the site of an ancestral Wichita city, Etzanoa, which flourished from 1450 to 1700 and had an estimated population of 20,000. In 1601, New Mexico Governor Juan de Oñate led an expedition across the Great Plains and found a large settlement of Indians he called Rayados. They lived along the Walnut River in the eastern part of Arkansas City. Extensive remains of Indian settlements have been found along six miles (9.7 km) of the Walnut River. The Rayados were the ancestors of the Indians later called Wichita.
Akhal TekeAmerican CreamAmerican WarmbloodAndalusianAppaloosaAppendixAraAppaloosaArabianAztecaBashkir CurlyBelgian DraftBelgian WarmbloodCanadianChincoteague PonyCleveland BayClydesdaleConnemara PonyDonkeyDraftDrumDutch WarmbloodFell PonyFjordFlorida CrackerFriesianGypsy VannerHackneyHaflingerHalf ArabianHanoverianHolsteinerHungarianIcelandicIrish DraughtKentucky MountainLipizzanLusitanoMiniatureMissouri Fox TrotterMorabMorganMountain PleasureMuleMustangNational ShowNew Forest PonyNZ SporthorseOldenburgPaintPaint PonyPalominoPaso FinoPercheronPeruvian PasoPintoPonyPony of the AmericasQuarabQuarter HorseQuarter PonyRackingRocky MountainSaddlebredSelle FrancaisShagyaShetland PonyShireSpanish MustangSpotted SaddleStandardbredSwedish WarmbloodTennessee WalkingThoroughbredTrakehnerWarmbloodWelsh CobWelsh PonyWestphalian

domain: geography


text: b'WEISS FINANCIAL RATINGS: THE DATABASENO NEED FOR EXPENSIVE SUBSCRIPTIONS TO MULTIPLE FINANCIAL DATABASES!Get it all in one place with WEISS FINANCIAL RATINGS SERIES ONLINE from Weiss Ratings and Grey House Publishing.INSTANT ACCESS TO RATINGS With a subscription to Weiss Financial Ratings Series Online, you can convert every workstation in your library into an instant online source of wealth-preserving information for your patrons. HELP YOUR PATRONS NAVIGATE TOUGH FINANCIAL DECISIONSA subscription to Weiss Financial Ratings Series Online makes sure that your patrons have the information they need to be sure they are working with the best and most financially stable organizations. Using our quick and easy navigation screen, your patrons will get immediate access to the Weiss Independent and Unbiased Ratings for: Life & Annuity InsurersStocks Property & CasualtyExchange-Traded FundsInsurersStock Mutual Funds HMOs & Health InsurersBond & Money Market Banks Mutual Funds Credit UnionsMedicare SupplementInsurersQUICK & EASY LOGIN AT THE LIBRARY OR AT HOMEWith IP access, logging in is fast and easy. Theres no need for cumbersome passwords. Your patrons will be able to access ratings simply by logging in at the library or your librarys branches. Access to Weiss Financial Ratings Series Online is also availablevia Remote Access, so your patrons can log in with their library card from home.Unlimited number of users and remote access are included in all subscriptions.PATRONS CAN CUSTOMIZE THEIR ACCESS, TOOPatrons can create a personal account to get email alerts about companies on their Watchlist, create and save their own personal screeners, and pick and choose which screeners they want to see when they log in. CUSTOMIZE YOUR ONLINE ACCESS PROMOTIONAL MATERIAL TO PROMOTE You can customize your subscription to Weiss FinancialYOUR LIBRARYS SUBSCRIPTIONRatings Series Online to build a database thats just Bookmarksright for your patrons. You can choose to add access Posters & Flyersto Banks, Credit Unions, Insurers, Stocks, Mutual WidgetsFunds, Financial Literacy Guidebooks or the Medicare Customized Press Releases for your Local NewspaperSupplement Insurance Buyers Guideto build a Choose Your Librarys Landing Page,platform thats just right for your library. with Recommended Companies in your State3(800) 562-2139 https://email@example.com firstname.lastname@example.org https://greyhouse.weissratings.com (800) 562-2139 4'

domain: finance


Shear Bond Strength Tester
We've reconfigured our usual components, precision solenoid, load cell, roller bearing slide, programmable stamp, keypad, LCD readout, etc, to make an affordable instrument for testing shear bond strength in dental adhesive specimens.
Proto-tech's Shear Bond Strength Tester reliably loads specimens up to 400N. The command module has a four-button controller that allows for variable loading rate and adjustable loading limit, and displays the maximum load of each specimen. The specimen holder accommodates 3/4 inch cylindrical specimens. The loading chisel is designed for 2mm diameter stubs, though fixtures for other specimen geometries are available on request. An immersion chamber with recirculating heating water bath is an available option too.
An expansion package with data logging desktop computer is also available. It has a footprint of 30x60 cm. The power requirement is 500W (1200W with recirculating bath).


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Oxygen Media will launch its first ever real-time video chat in a Google+ Hangout during a reality broadcast. Fans can join "The Face" cast members Naomi Campbell, Karolina Kurkova and Coco Rocha, as well as the show’s host Nigel Barker. Segments of the chat will be broadcast live on the network and also presented in its entirety on Oxygen.com,Google+ and YouTube on Wed. Feb. 6 at 8:00 p.m. ET.
The chat will be moderated by SuChin Pak, Chief Correspondent for DailyCandy, the popular women’s fashion and lifestyle digital media company, Google+ Hangout will serve to engage a global audience in a live conversation and offer sneak peeks of the highly-anticipated series premiering on Tue., Feb. 12 at 9:00 p.m. ET.
Fans of “The Face” can access exclusive content and join the conversation on the show’s social accounts, including Google+, Facebook, Twitter, Tumblr, and Pinterest. Fans may tweet or read about the series using #TheFace hashtag on Twitter and Google+. The supermodel coaches and host can also be followed via Twitter using @NaomiCampbell, @KarolinaKurkova, @CocoRocha and@NigelBarker.
Related links for "The Face"
- Naomi Campbell, Karolina Kurkova and Coco Rocha bring you 'The Face'
- Oxygen launches 'The Face Challenge' on YouTube
- Join 'The Face' cast in a Google+ hangout on Feb. 6 at 8:00 p.m. ET
If you'd like to receive an email each time I add new content to the site, click on the "Subscribe" link near the top of the page to get added.
Looking to contact me? Feel free to send Shelly an email.


The GRMN Sports FR Concept Platinum seen at the Tokyo Auto Salon over the weekend wasn’t the only offering from GRMN, Gazoo Racing’s customisation and tuning arm. There was also the GRMN Yaris Turbo Concept, a race-fettled version based on a three-door Australian-spec’d model.
The car is powered by a turbocharged 1.5 litre mill offering 148 hp and 205Nm of torque. Exterior and interior changes include new aluminium wheels, sports seats, race pedals and a modified rear bumper, among other things. Also tucked in is a tuned suspension, uprated disc brakes and a bodyshell that has been strengthened for track use.


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Perth 1 Day Tour
Return Airport transfers in Darwin, Perth on seat in coach basis
Australia Visa Charges
Travel Insurance up to age of 60 as per tour duration.
Overseas Travel Insurance
Anything not mentioned in the Inclusion
Tips & gratuities for drivers & guides
Waiting or additional transfer fees if clients miss shuttle transfers or transfers booked for a specific time
Meals not mentioned in the Itinerary or Inclusions
Items of a personal nature, such as telephone bills, room service, laundry, etc
Airfare and airport taxes as per itinerary.
Day 1 – Hello, Darwin! Arrive Airport and exchange to lodging, rest of the day at relaxation
Perth 1 Day Tour Today is the start of your excursion to Darwin, situate in mentor exchange to accommodation.Overnight remain in Darwin. after breakfast, a mentor will lift you up from your lodging for your exchange to 69 Mitchell Street, the flight purpose of your visit. At 6.30am join your mentor to initiate a multi day Kakadu and Litchfield National Parks visit.
Leave Darwin and travel crosswise over Adelaide River and Marrakai Plains into World Heritage-recorded Kakadu National Park. Covering somewhere in the range of 20,000 square kilometers, Kakadu is Australia’s biggest and seemingly most popular national stop. Kakadu’s immense wetlands and old slope are home to one of Australia’s biggest and most various fledgling populaces, supporting up to 33% of all Australian winged creature species.
Visit shake craftsmanship destinations at Nourlangie Rock – the name Nourlangie’ is an anglicized variant of Nawurlandja. Appreciate lunch before boarding the Yellow Water billabong journey to watch saltwater crocodiles and the vivid scope of birdlife that this locale is eminent for.
Late toward the evening visit Warradjan Aboriginal Cultural Center and view the enlightening showcases and displays portraying the conventions of the Aboriginal individuals in Kakadu. A discretionary 50 minute grand trip over Kakadu is accessible today (possess cost).


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Black teddy quilted jacketSale!
DESIGNER ‘S NOTES
Our new winter jacket is the latest oversized outerwear must-have. It’s quilted throughout and detailed with splits at the side. Features a high collar at neckline with statement white buttons and white front zipper. Made from warm black capitone rombus fabric, has a roomy silhouette and large pockets.
- Pes- Ny
- Color- Black with white details
- One size
HANDMADE IN GREECE

domain: design


Currently, various kinds of technology are used to stabilize the resonator frequency of crystal oscillators: VCXO, TCXO, OCXO, etc. They have different performance to meet customers' various demands.We illustrate their characteristic as follow so that they can help customers choose correctly accroding to design.
1.VCXO: Voltage Controlled Crystal Oscillator. The output frequency of a VCXO can change with a change of control voltage. The amount of frequency shift with a given voltage change is highly dependent on the oscillator circuit. VCXOs are widely used in telecommunications, phase locked loops, instrumentation, and other electronic equipment when a stable but electrically tunable oscillator is required. The VCXO typically employs a varactor diode to vary the frequency of oscillation by applying control voltage.
2.TCXO: Temperature Compensated Crystal Oscillator. A TCXO adjusts its frequency for temperature variation. As a VCXO is an integral part of a TCXO, the general approach is to use a network of temperature sensors designed to appropriately bias the voltage control of the VCXO for a range of temperatures. The correction voltage is usually applied to a varactor diode in the crystal circuit so that the crystal frequency can be varied by a small amount.
3.OCXO: Oven Controlled Crystal Oscillator. An OCXO offers higher stability and better phase noise specification than other crystal oscillators do. The crystal and other temperature-sensitive components are in a stable oven, which is adjusted to the temperature at which the crystal’s frequency change is the smallest over the temperature range of the oven. Additional circuitry is required to control the oven temperature.


At @Fraser Gartshore’s last livestream, he played this beautiful piece by Maurice Duruflé that was originally composed as an exercise in harmony for his teacher, Jean Gallon. I do have a copy of this music titled “Chant Donne” but this copy is perhaps the most definitive!
As Fraser states in his rendition:
Many years ago, on a trip to Paris, I got my hands on this piece (I won’t tell you how!) and made a copy for myself – the version I used for this recording. After researching the composer’s intentions (talking to friends, family and pupils of the great Duruflé, including the late, great Pierre Pincemaille, organist of St Denis in Paris), I believe this is still how he intended this piece to be played. Other editions and arrangements of this piece do exist, but they are based on later, amended versions of the composition.
This morning I played it live during the offertory at Mass. Here’s the specification of the organ in case you are interested: https://www.ohta.org.au/confs/Albury/AlburyRC.html
Video shot on an iPhone 12 with external audio recorder.
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Comprehensive stress analyses indicate that plastic yield, fracture initiation near or between packers or from a perforated hole are all possible during hydraulic fracturing. Furthermore a fracture can be created from one of the perforated holes located in deviated wellbore subject to three in-situ stresses regime. The corresponding fracturing pressure can be determined based on the orientation of this perforated hole. While a plastic yielding process on a wellbore or perforated hole surfaces may prevent a fracture from initiating, a fracture or fractures may initiate from a near packer region or at an oriented perforation when different completion strategies utilized.
Staged fracturing strategy has been widely used in U.S. and Canada for low-permeability tight formations. Fractures or a fracture network may be initiated from an open hole or perforations. Depending on the layout of a horizontal well, the perforation orientations, clusters of perforations, and their distances to the packers in each stage, creations of fracture networks and fractures initiation are of great interests to engineers for productivity and surface control purposes. We normally calculate the tangential stresses near a wellbore or perforation for open and cased holes, respectively. Whether a transverse, radial, or arbitrarily angled fracture can be generated, if a fracture can be initiated at a specific wellbore location or from one of the perforations (in the middle, at the edge of the packers or from one of multiple perforated holes) remains unanswered. These issues must be addressed by considering the critical stresses at the wellbore and perforations once we shall have a clear pictures if plastic yield occurs prior fracturing, or which perforation is oriented at an optimum position referring to the in-situ stresses, and if the near packer regions are heavily influenced by the in-situ stresses. Both a 3D finite element model and an analytical poro-elastoplastic model are developed to analyze the corresponding effective stresses. These models focus on regions near the packer or the oriented perforations. They evaluate the plastic yielding and the stresses in these areas in order to determine the location and the pressure at which a fracture can be initiated or no tensile fracturing is possible at all due to plasticity.


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I Predacon't believe we have Emily on our show! We'll have to Maximize the time we have with her to (fill this spot in later with an equally witty pun). EDIT: Well, I couldn't think of one, so go ahead and fill that in yourself.
Opening theme is Master of Puppets performed by Viktoriya Yermolyeva.
Closing theme is Come Clean (Instrumental) by Hilary Duff


text:
HDMI Splitter allows set-top boxes, DVD players, D-VHS players and other HDTV
devices with a HDMI output to be connected to up to two HDTV displays. HDMI
splitters can be daisy chained to accomodate more displays.
to four HDTV displays at the same time to the same video source

domain: entertainment


It’s another episode of the Food Tech Show!
This week I got together with the Spoon’s Chris Albrecht and Catherine Lamb to talk about some of food tech’s most interesting stories of late, including:
- The cancellation fo the foreign sourcing expo by the Housewares Show (aka Inspired Home) because of coronavirus
- Cargill wades into the increasingly crowded plant-based burger market
- Starbucks and McDonalds take the NextGen Cup challenge
- Drinkmate’s effort to help us carbonate drinks on the go


Influence of stirring speed on SiC particles distribution in A356 liquid
A straight-blade mechanical stirrer was designed to stir A356-3.5vol%SiCp liquid in a cylindrical crucible with the capability of systematically investigating the influence of rotating speed of stirrer on the distribution of SiC particles in A356 liquid. The experimental results show that the vertic...
|Main Authors:||, ,|
Foundry Journal Agency


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[NAFEX] Apple Scab
gardendiva at charter.net
Fri May 30 21:42:18 EDT 2008
Dennis Norton wrote:
I know that Ashmeads's Kernel is moderately susceptible to scab, but I'm not sure about Pitsmaston Pineapple. If you had scab last season and did not remove the overwintering leaves or any mummies from the ground, you will definitely get scab this season. Have you used any sprays for scab this season?
I religiously remove all fallen leaves and debris under the trees. They sit on 9-foot-wide beds, heavily mulched with shredded cedar bark. I'm an organic grower and do as little as possible to avoid spraying the fruit. That's why I bag. I use dormant oil and lime sulfur at pruning, need seed oil spray at pink bud and again at petal fall. Other than a clean-up spray of neem seed oil in late autumn, that's all I do. Despite the scab, the trees look very healthy and produce blemish-free, tasty fruit. Is there something else I could spray that qualifies as organic?
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domain: agronomy


text:
Add to Basket
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Van Cleef & Arpels sapphire and diamond ring. Set with three graduating round brilliant cut diamonds in open back claw settings with a combined approximate weight of 0.45 carats, further set with three graduating round old cut natural unenhanced sapphires in open back claw settings with a combined weight of 0.60 carats, to a fancy openwork rope twist design, the conforming band tapering to a solid ridged D-shape shank. Marked 18 carat yellow gold, maker's mark 'VCA', numbered '89183', French, circa 1960.


Replace the original source of your enlarger (often a halogen lamp or incandescent lamp, very energy consuming, and increasingly difficult to obtain) by a modern LED panel, stable and very energy efficient.
The HEILAND head works with LEDs of three primary colors: red, green and blue. This allows to obtain a white light for focusing, to have an inactinic light (without filter) and to vary the contrast in a very precise way, by 0,1 grade.
In addition, the wavelengths are perfectly designed to be within the sensitivity spectrum of the multi-grade paper, making the light output as efficient as possible.
The light intensity is also easily adjustable over two stops, to cope with all paper sensitivities.
There are three ways to control the LED head:
- The classic, simple control module. If you are used to multigrade heads and like to keep control, this is the solution for you.
- The SPLITGRADE control module. All adjustments are calculated by the SPLITGRADE module.
- The COLOR control module. For color printing applications, or for black and white printing, independently managing the MAGENTA and YELLOW channels
Advantages of this technology:
- Full gradation control, from 00 to 5, in 0.1 grade increments;
- Light intensity control on two diaphragms
- Strong decrease in the number of replicas
- No ignition latency;
- No ventilation, so no vibration, no heat and no noise;
- No heating of the negative, which remains flat, even with glassless holders;
- Integrated inactinic lighting: easy paper positioning;
- Very efficient light quality, allowing short exposure times, despite low electrical power;
- Variable light intensity;
- No lamp replacement required;
- The white light makes it easier to focus;
- Perfect homogeneity of the light on the whole surface;
- Ideal for enlarging valuable archival negatives because there are no heat marks;
- Solid construction.
Optional: SPLITGRADE system:
The Splitgrade system consists of an analysis module and a densitometric probe. The system calculates for you the exposure time and contrast for an optimal result, obtained quickly.


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She will endorse a slew of the company's mobile handsets. As part of her association, Sunny recently completed her first television commercial for the brand, and shot for it in Bangkok.
"I am really impressed by their ideology of bringing out mobile technology for the masses and personally feel connected with their vision," said the 30-year-old.
The Punjabi-origin actress, whose real name is Karen Malhotra, became popular in India after her stint on the fifth season of reality show 'Bigg Boss'. During the show, filmmaker Mahesh Bhatt offered her 'Jism 2', and she accepted it.
The movie, a sequel to 2003 film 'Jism', is being directed by Pooja Bhatt. It also features Randeep Hooda and Arunoday Singh.
Bollywood celebs show you colours for Navratri 2017
Photos: Kareena Kapoor Khan, Kangana Ranaut at the Mumbai airport
Birthday special: Chris Gayle and his multiple records in cricket
In Pictures: 17 dreaded on-screen villains of Bollywood
'Ramayan' director's great granddaughter Sakshi's hottest photos


The berlin representation of the brand from Wolfsburg started their production in 2002. The model was born with aspirations of becoming the reference model of this segment compared to the options of BMW, Mercedes or Audi. Since then, the Volkswagen Phaeton has not ceased to occur. Until today. The German brand has announced that ends production of the Phaeton in its factory in Dresden, Germany. A news that went forward to the end of last year, and who have now made it official.
After 14 years, the last drive of the Volkswagen Phaeton has come out of this plant German with destination China. The same looks black in color and hides under the hood the V8-4.2-liter with 335 HP associated to an automatic gearbox and all-wheel drive 4Motion. In total have been manufactured 84.000 cars, a luxury saloon to say goodbye these days.
The plant of Dresden had 500 employees, of which 100 will remain in this factory, being the rest destined for other factories. Between 29 march and 8 April, these facilities remain closed, a few days that will be used by the company to work on the same floor. And it is that they want to turn it into a showcase to show their latest developments in electric mobility and digitization. According to Volkswagen, “visitors will be able to experience it from very close in a fun, interactive and informative the future of mobility”.
The next-generation Volkswagen Phaeton will be all-electric, a decision that was confirmed after the scandal of the dieselgate. it is not Yet known whether the plant in Dresden will produce vehicles, being able to get models from Audi, Bentley or Porsche.
Source – Porsche
From 85.600 euro


text: Since this is one of my first posts here at BHW, I wanted to give back and contribute to the forum. I have noticed a lot of questions regarding offshore banking and a lot of false information being passed around. In this industry if you aren't careful the consequences can be very serious. Since I am very familiar with this industry I wanted to pass along some knowledge. I'm not going to extol the virtues here of having a bank account in a country other than your own, that is for another post, but I wanted to provide a (very) brief overview of some of the different jurisdictions that are good for banking, depending on your needs. Safest Jurisdictions If you have six figures to park in an account and not really have a desire to touch it, there are a few chances that stand heads and shoulders above the rest, regardless of your country of citizenship or residence. Switzerland Switzerland has been notorious for the past several hundred as a tax haven, however its not quite what it used to be. Its no longer a haven for money launders and tax evaders like it was before, however when used properly (read those last three words again) it can be a remarkably powerful jurisdiction. Very modern, and some of the most (if not THE most) stable banks are located here. No bank in Switzerland has ever gone under. Ever. They simply get absorbed by a larger bank. There are many good choices in Switzerland, but not as much as there used to be. If you are a US citizen your options are more limited and getting a corporate account opened is very difficult regardless of your citizenship (unless your company is domiciled in Switzerland). Singapore Singapore is a real up and comer. It is fast becoming the ?Switzerland of the East? because of its reputation for discretion and the high quality of the bankers and banks located there. Singapore is frustrating the rest of the world for its general non-cooperation with exchange of information regarding tax matters. Like Switzerland no banks have ever failed in Singapore. A great choice if you are receiving funds from Asia, there really is no better choice. Hong Kong comes close but in general for Asia, Singapore is the way to go. Best Jurisdictions in the EU Cyprus Bank accounts are generally easy to open, lots of good banks located here. Has safer banks than a lot of the other choices in Europe. Latvia Seems to be a popular jurisdiction lately, and rightly so. Accounts (both personal and corporate) are easy to open and the fees are very low in general. The banks are not as well capitalized as the ones in Cyprus but the entire financial system in Latvia is extremely modern. Notes about the Caribbean Islands Much fuss has been made in the past several decades about tiny Caribbean islands being easy for money launderers and terrorists to move their money through. Not so anymore. Almost all of the islands are virtually useless for banking anymore, and not just because of the loss of privacy. Many jurisdictions have banks which are not well capitalized. The two best jurisdictions for ease of account opening, banking privacy, and low cost are St. Vincent and the Grenadines and St. Kitts and Nevis. Of these two if I absolutely had to choose I would go with St. Vincent and the Grenadines, especially if I was a US citizen, but that being said both are good choices. Caribbean jurisdictions you should absolutely avoid include Cayman Islands, Belize, Dominica, St. Lucia, and all of the other British dependencies. Also included in this list would be Panama, which was popular for a while but recently signed away its banking secrecy in a Tax Information Exchange Agreement (TIEA) late last year. Now that it has to compete head to head with other jurisdictions, it is no longer a recommended choice. Obviously there are plenty of other choices but this primer is not meant to include everything. Hope this was informative.

domain: economics


text:
If you’re under-going a long distance relationship break up, then get no doubt spent a lot of time planning on your marriage. How long achieved it last? Do you always ponder over it to be a thing distinctive or would you just leap with it with eye closed? Prolonged distance connections can be draining on a relationship, but they also offer plenty of great benefits and ideas. Here are some lengthy distance romance ideas which can be sure to ignite romance into your life.
Primaly to start when considering long distance romantic relationship ideas is always to look at your own practices. Spending time apart from each other can provide you with that new point of view on your additional greatest adores and help you realize more fully the time you do spend together on a daily basis. That’s every well and good, but it does not negate the fact that long distance relationships are hard work. When you take more time apart, you are likely to gain a lot more realistic concept of how much per day at work plus your own your life can actually take, giving you an idea of what your way of life is like without your partner.
Up coming, couples who had success in long length relationships should give their partner a duplicate of their date. If they are married, this appointments will show each partner’s timetable for the last 90 days as well as possible long term dates. This will help both companions understand precisely what is ahead of these and so why they can be feeling the way they do. This calendar also needs to include paperwork about virtually any problems or concerns, and even future incidents. After all, if you are planning a vacation or possibly a honeymoon, you may want to make sure that your partner is happy with the plan.
Romantic endeavors is one of the most common reasons persons seek out romantic, sweden girl for marriage extended distance human relationships. After all, there is something deeply nourishing about viewing a movie at the same time, whether that is certainly from a show theatre or over a drive home. Couples exactly who enjoy finding movies along tend to be happier overall in their romances than couples who under no circumstances take the time to be able to see each other. Of course , if the couple just ever watch movies together if the movie star reaches the best shape possible, they will rarely, when, achieve the degree of intimacy that is required for permanent relationships.
Perhaps the most affectionate of very long distance romance ideas is always to simply spend time together. A lot of persons choose to use weekends on the beach, ingesting the scenery while consuming popcorn and enjoying the sun’s rays. Others prefer to take extended walks along a river or consider long car trips. These people realize that they are more content, both bodily and emotionally, when they can spend time together and simply be each other’s best friends. After all, merely that what every superb relationship is all about?
Of course , the most romantic of long length relationship concepts is to have two people so, who love the other person deeply and devote the lives to one another. Some people make it a point out visit the different person regularly, whether they will be in the same city or thousands of kilometers away. Different couples like spending time for a ski resort or a hot air balloon. A lot of couples experience going to Europe for an adventure getaway or spending some time with a adorable local person. No matter what your idea of enchantment is, the ideas previously mentioned are sure to ignite romantic imaginations and deliver a fresh sparkle to your relationships.

domain: psychology


The Titan comes in an electric model called the E-Titan for $3529.00.
E-Titan is a modified 9 Speed Titan- It uses an electric motor system integrated system. It features a 350W Geared Hub Motor, a special Cranket with sensors, a 36V 13 AH Rack Mounted Battery with built in light, and a Digital Display. With this system you can choose between throttle only or 6 different levels of pedal assist.The 36v 13AH battery is much larger than other manufacturers use and will give you a much longer range- about 30 miles electric only and up to 75 miles using pedal assist.
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