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Comparative antilipidemic effect of N-acetylcysteine and sesame oil administration in diet-induced hypercholesterolemic mice
Lipids in Health and Disease volume 9, Article number: 23 (2010)
There is an increasing number of novel antilipidemic therapies under consideration. The putative hypolipidemic effect of N-acetylcysteine (NAC) and sesame oil was studied in a mouse model of dietary-induced hypercholesterolemia.
Male C57bl/6 mice were assigned to the following groups: (NC) control group, (HC) group receiving test diet supplemented with 2% cholesterol and 0.5% cholic acid for 8 weeks, (HCN) group receiving the test diet with NAC supplementation (230 mg/kg p.o.) and (HCS) group fed the test diet enriched with 10% sesame oil. Total serum cholesterol, LDL-cholesterol, HDL-cholesterol and triglycerides were assayed at the beginning and at the end of the experiment. Total peroxides and nitric oxide (NO) levels were measured in the serum at the end of the experiment. Hepatic and aortic lesions were evaluated by haematoxylin-eosin staining.
Higher serum levels of total and LDL-cholesterol were recorded in all groups fed the high cholesterol diet. The HCN group presented reduced lipid levels compared to HC and HCS groups. No differences were observed between HCS and HC groups. Peroxide content in serum was markedly increased in mice consuming high cholesterol diet. NAC and sesame oil administration led to a significant decrease of serum lipid peroxidation in the levels of control group, whereas only NAC restored NO bioavailability. In terms of liver histology, the lesions observed in HCN group were less severe than those seen in the other high cholesterol groups.
Co-administration of NAC, but not sesame oil, restored the disturbed lipid profile and improved hepatic steatosis in the studied diet-induced hypercholesterolemic mice. Both agents appear to ameliorate serum antioxidant defense.
Hypercholesterolemia in combination with raised LDL-cholesterol concentration represent major risk factors for the development and progression of atherosclerosis and consequently of cardiovascular disease . Based on this evidence, scientific research is targeting the discovery of new drugs with hypocholesterolemic effects. Plant based dietary therapies and natural food components are being proposed nowadays for the prevention of dyslipidemia. The antioxidant N-acetylcysteine (NAC), an organosulfur from Allium plants and sesame oil appear as promising lipid-lowering agents in recent literature.
NAC is a hydrophilic cysteine-containing compound naturally formed in Allium plants such as garlic and onion. The intake of this compound was found to effectively decrease high saturated fat-induced triacylglycerol (TAG) and cholesterol accumulation in mice livers. Moreover it has been shown to protect the liver against high fat-induced oxidative damage .
Sesame belongs to the species Sesamum indicum (Pedaliaceae family). It has been extensively used as a traditional food in the orient but it is also considered to have important medical properties. There is abundant data available from different studies mainly on rodent species supporting the hypolipidemic action of many components of sesame seed and its oil [3, 4]. Satchithanandam et al  concluded that the absorption of lymphatic cholesterol in rats fed diets containing sesame oil was about 50% less than by rats fed a control diet. Additionally, administration of non defatted sesame seed powder in hypercholesterolemic albino rats, improved their antioxidant status and significantly decreased plasma lipids . To our knowledge there is no literature-based evidence of the potential hypolipidemic effects of sesame oil administration without substitution of other fat dietary compounds.
Therefore, the present study was undertaken to determine whether NAC and sesame oil administration would have beneficial hypolipidemic and antioxidant effects in a murine model consuming a chow diet, enriched with cholesterol and cholic acid.
12-week-old C57bl/6 male mice (Mus musculus) were obtained from Alexander Fleming Institute (Vari, Greece) and were acclimatised for one week before the experiment started. The animals were housed under conditions of controlled temperature (23 ± 2°C) and humidity (60%) with 12 h light/dark cycle. The experimental protocol was reviewed and approved by the Veterinary Directorate of Athens Prefecture and by the Ethics Committee of the Medicine School of the National and Kapodistrian University of Athens, in accordance with ethical recommendation of the European Communities Council Directive of November 24, 1986 (86/609/EEC).
A total of 25 mice were divided into the following 4 experimental groups:
NC (n = 5): normal control with basal diet (4RF25, Mucedola, Milan, Italy); HC (n = 5): animals that received the normal chow diet supplemented with cholesterol (2%) and cholic acid (0.5%) (high cholesterol diet) for 8 weeks; HCN (n = 7): animals fed the high cholesterol diet with NAC (Flumil Antidoto, IΦET, Greece). NAC was administrated p.o. in drinking water in a concentration tested to result in a daily uptake of approximately 230 mg/kg body weight. The dosage of the drug was determined according to previous reports on rodents [6, 7]. HCS (n = 8): mice which received the high cholesterol diet enriched with 10% sesame oil for the same period. Sesame oil was obtained from a local health food store. High cholesterol content was used in the high cholesterol diet in order to obtain hypercholesterolemia after a short follow-up period. Cholesterol and cholic acid (approximately 95% and 99% purity respectively) were obtained from Sigma-Aldrich (Germany). The high cholesterol diet was prepared weekly by dissolving cholesterol in diethyl ether (without butylated hydroxytoluene as inhibitor) and cholic acid in methanol and thoroughly coating with these solutions the pellets of standard chow cut in small pieces. After solvent evaporation, the chow was stored at -20°C until use. Fresh food from the freezer was provided to the animals every day while food remaining from the previous day was removed. The appropriate quantity (corresponding to daily uptake) of this high cholesterol diet was first weighed, mixed thoroughly with the appropriate amount of sesame oil and then dried and weighed again in order to achieve a final concentration of sesame oil in mice diet at a level of 10%.
Mice had free access to eat and drink throughout the study. During the 8-week period, food consumption was monitored daily, whereas the amount of water consumed daily was also recorded in HCN group. Mice were weighed once a week.
Blood collection and serum lipid measurement
Blood samples of mice were collected at the beginning and at the end of the experimental period (at 9:00 am, after a 12-h fasting period), using capillary tubes introduced into the medial retro-orbital venous plexus under light ether anaesthesia. A quantity of about 150-200 μl of blood was collected from each mice at the beginning of the experiment. Bigger quantity of blood (400 - 500 μl) was collected at the end of the experiment and before mice's euthanasia. Serum was separated by centrifugation at 3000 rpm for 10 min and was stored at -80°C until analysis.
Serum concentrations of total cholesterol and of triglycerides were determined using the enzymatic PAP commercial kit ("biosis" - Biotechnological Applications, Athens, GR) and HDL-cholesterol was determined with a cholesterol enzymatic photometric method. LDL-cholesterol was determined by the mathematic model "LDL-cholesterol = Total Cholesterol- (HDL-cholesterol + Triglycerides/5)".
All samples were analyzed at the Laboratory of Experimental Surgery and Research of the Medical School of Athens (Athens, Greece).
Serum lipid peroxidation - NO levels determination
Total peroxide concentration in collected serum was assessed photometrically (PerOx kit, Immundiagnostic AG, Bensheim, Germany). Total serum nitric oxide (NO) was calculated based on the enzymatic conversion of nitrate to nitrite by nitrate reductase, using a commercial kit (Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit, R&D SYSTEMS, Minneapolis, MN, U.S.).
At the end of the 8-week period, animals were euthanized under ether anaesthesia with their livers and aortas dissected immediately for further histopathological analysis. Part of the livers and aortas were fixed in 10% formalin at room temperature. The tissues were then embedded in paraffin, sectioned and mounted on glass microscope slides. The sections were stained with hematoxylin-eosin and examined blindly by two independent researchers using light microscopy. More precisely, the liver was evaluated as had been previously described by the Pathology Committee of non-alcoholic steatohepatitis Clinical Research Network . The histological features were grouped into 4 broad categories: steatosis, ballooning, portal inflammation and lobular activity. A score from 0 (absence) to 3 (severe lesion) was assigned to each parameter.
All of the analyses were performed using the SPSS statistical software. The results were expressed as mean values ± standard errors and analyzed for statistical significance by One Way ANOVA test. Liver lesions were demonstrated as mean score ± standard errors for steatosis, ballooning, portal inflammation and lobular activity. Statistical significance was set at P < 0.05.
Body weight and food and water intake
In the present study the initial body weight for all groups was similar. At the end of the 8-week study the mice of the HC group presented reduced body weight compared to the animals in the NC (P = 0.027), HCN (P = 0.018) and HCS (P = 0.002) groups (Table 1). A statistically significant increase was observed in food consumption in the HCN group relative to the NC (P < 0.001) and HCS (P < 0.001) group. The daily water volume consumed by the mice in HCN group was 4.36 ± 0.15 mL.
Serum lipid profile
At the initial blood sampling, no differences in lipid levels (except LDL-cholesterol, Table 1) were recorded among the groups. Feeding of the high cholesterol diet containing 2% cholesterol and 0.5% cholic acid for the period of 8 weeks resulted in the development of hyperlipidemia in experimental mice groups compared to the control group. Serum total cholesterol and LDL-cholesterol levels (Table 1) were significantly increased in the three groups fed the high cholesterol diet compared to the NC group (P < 0.001).
NAC co-administration markedly reduced total cholesterol (P = 0.013) and LDL-cholesterol (P = 0.012) levels compared to the HC group. Although there was a slight decrease in total cholesterol and LDL-cholesterol in HCS compared to the HC group, this reduction was not statistically significant. HDL-cholesterol levels were found to be decreased in HCS group compared to NC group (P = 0.006), whereas there was no difference between the other groups. Lower concentrations of triglyceride levels were observed in all groups fed the high cholesterol diet in comparison to the control group (P < 0.001).
Serum lipid peroxidation - NO levels determination
The evaluation of peroxidation products showed that peroxide content in the serum was markedly altered in the HC group related to the other three experimental groups (Figure 1). Both NAC and sesame oil administration reduced serum peroxidation to the levels of the control group, improving antioxidant status (Figure 1). NO serum levels (Figure 2) were reduced in the HC group in comparison to the control group, whereas the difference was marginal between the HC and the HCS group. Nevertheless, the HCN group presented significantly elevated NO levels compared to the HC group preserving it at its normal level for mice.
Liver and aorta histopathology
Hematoxylin-eosin stained liver samples, obtained from the groups fed the high cholesterol diet, showed signs of liver steatosis and inflammation (Figure 3). A significant increase in accumulation of fat droplets was observed in the livers of the HC and HCS groups compared to the NC group [HC and HCS groups vs NC, (P = 0.001) and (P < 0.001) respectively] (Table 2). This increase was apparently suppressed in HCN group of mice in comparison to the HC group (P = 0.001), whereas no difference was observed between HCN and NC groups (P = 0.763). Moreover, the architecture of hepatocytes in the HCN group was found to be more similar to NC group compared to that of the HC and HCS groups (Figure 3). Ballooning of hepatocytes and lobular activity were observed in liver tissues of all mice in the HC and HCS groups, as compared to only two of the seven animals of HCN group. Neither NAC nor sesame oil administration improved hepatic inflammation. The grade of fatty liver disease was considered as "mild" in three of the seven mice in HCN group. In five mice from the HC group, the grade was "severe" in three, "moderate" in one and "mild" also in one mouse.
In evaluating the total examined parameters in hepatic tissue of mice, we conclude that NAC administration improved liver histology whereas diet supplementation with sesame oil had negative effects.
No fatty streaks or plaques were recorded in aortic tissues of all experimental mice (data non shown).
Hypercholesterolemia is one major risk factor for the development of atherosclerosis. The present study focused on the possible antilipidemic effect of NAC and sesame oil administration in hypercholesterolemic mice. To the best of our knowledge, this is the first study that investigated NAC and sesame oil action on the lipid profiles of mice consuming a chow diet enriched with cholesterol and cholic acid.
Cholesterol-cholic acid feeding has often been used to raise cholesterol levels in plasma and tissues of experimental animals [9, 10]. The experimental diet followed in the present study resulted in elevated total cholesterol and LDL-cholesterol levels in all mice receiving it. Triglyceride levels decreased in all groups fed the high cholesterol-cholic acid diet. This finding is in accordance with previous studies in mice and rat groups fed with diets containing cholic acid or cholate [11, 12]. Furthermore, according to the study of Kamisako et al.  there was a slight decrease in triglyceride levels of rats fed a diet containing 1% cholic acid as compared to their control counterparts. Li et al.  assumed that the triglycerides reduction in their study might be a consequence of the inhibition on 7a-hydroxylase activity due to the presence of the cholate in the experimental atherogenic diet, something that is not in accordance with Beigneux et al.  who suggested that inhibition of 7a-hydroxylase activity leads to the increase of triglyceride levels.
Hypercholesterolemia enhances the free radical generation in various ways  and is generally associated with an increased production of oxygen radicals such us superoxide anion radical . NAC in our study had beneficial effects concerning lipid profile. The administration of this antioxidant largely prevented the diet induced increase in serum lipids (total cholesterol and LDL-cholesterol levels).
NAC has been shown to reduce cholesterol levels in plasma and liver in Balb/cA mice consuming a high saturated fat diet . Krieger et al.  mentioned a slight reduction in plasma lipid fractions by means of NAC supplementation in hypercholesterolemic LDL receptor knockout mice. Therefore, other researchers indicate a beneficial effect of NAC on dyslipidemic profile of rats fed a high sucrose diet or of rats given standard chow and 30% sucrose in their drinking water [17, 18]. Putative mechanisms accounting for the lipid-lowering effects of NAC might be related to its antioxidant properties. NAC in our study led to reduced serum peroxide content. The maintenance of the normal structure of lipoprotein receptors is indispensable for their function, improving the cellular uptake of serum lipids from the blood. Reactive oxygen species produced during oxidative stress react with lipoproteins to produce oxidation states, diminishing the cellular uptake of lipids from the blood [17, 19, 20]. Thus the antioxidant action of NAC might contribute to elevated cellular lipid uptake, resulting in the decrease of serum cholesterol levels.
According to Lin and Yin , the lipid lowering action of NAC in mice consuming a high fat diet is attributed partially to the suppression of mRNA expression of three lipogenic-related enzymes (malic enzyme, fatty acid synthase and 3-hydroxy-3-methylglutaryl coenzyme A reductase).
NO is considered as an important vasoactive substance which protects the integrity of the blood vessels. Hu et al.  concluded that plasma NO levels declined in rabbits fed a high fat diet. Similar to this study, we observed that our experimental high cholesterol-cholic acid diet suppressed serum NO levels, whereas NAC administration raised NO bioavailability and maintained it at the normal level. Thus NAC might display an essential protecting role for the normal function of arteries.
Our results concerning hepatic steatosis and inflammation are consistent with those of previous investigations . As previously described, NAC administration suppresses hepatic lipid infiltration in hepatic tissues of mice fed high fat diets . The hepatoprotective action of NAC might be associated with its antioxidant action, as mentioned above.
Similar to the findings of Sener et al. , the cholesterol-cholic acid diet used in our study did not lead to fatty streak or plaque formation in aortic tissues of mice. This finding was partially anticipated, as our test diet consisted of the regular chow diet (3.5% fat) enriched with cholesterol and cholic acid without additional fat. In contrast, diets used for the initiation of atherosclerosis in wild-type mice like Paigen diet [24, 25] have a final fat composition of more than 15% (in weight percentage).
Sesame oil supplementation, at a level of 10% in the test diet, failed to restore the disturbed lipid profile caused by the high cholesterol-cholic acid diet, despite the decrease in serum peroxidation. Total cholesterol and LDL-cholesterol levels exhibited a decrease by sesame oil administration. This preventive effect however could not be statistically confirmed, probably due to the limited number of available observations or/and to the elevated caloric value of the sesame oil modified diet. In addition, we observed that sesame oil co-administration enhanced hepatic steatosis and inflammation.
Administration of dietary defatted sesame flour to rabbits did not protect against cholesterol-induced hypercholesterolemia, but decreased the susceptibility to oxidative stress (lower serum and liver lipid peroxidation) perhaps due to the antioxidant activity of sesaminol . Bhaskaran et al.  reported a significant reduction in plasma cholesterol, LDL-cholesterol and triglycerides levels in LDLR-/- mice when their atherogenic diet was reformulated with the same level of sesame oil. In our study, sesame oil was added to the test diet without fat substitution. The additional calories of the sesame oil diet might resulted in the absence of hypolipidemic response, as mentioned above.
In an earlier study, an increase was shown in the hyperlipidemic status of rabbits fed a hypercholesterolemic diet enriched with olive oil , whereas Acin et al.  concluded that dietary cholesterol suppresses the ability of olive oil to delay the development of atherosclerotic lesions in mice, negatively influencing their plasma lipid parameters. Therefore we can assume that cholesterol addition, like in the case of olive oil, in part suppresses the putative hypolipidemic action of sesame oil.
In summary, even though sesame oil presented antioxidant properties, it did not show significant hypolipidemic activity in mice consuming a chow diet enriched with cholesterol and cholic acid. NAC administration was able to improve lipidemic status in the serum, to maintain liver physiology and to reduce serum peroxides probably by ameliorating the efficiency of the antioxidant defence system of experimental animals. Moreover, NAC might exert preventive antiatherogenic activity by means of raised serum NO levels.
Co-administration of NAC, but not sesame oil, restored the disturbed lipid profile and hepatic injury in the studied hypercholesterolemic murine model, although both agents enhanced serum antioxidant capacity. Thus NAC might represent a beneficial alternative for lowering cholesterol levels in humans during clinical practice.
group receiving test diet supplemented with cholesterol and cholic acid
group receiving the test diet with NAC supplementation
group fed the test diet enriched with sesame oil
low density lipoprotein
high density lipoprotein
Prasad K, Kalra J: Oxygen free radicals and hypercholesterolaemic atherosclerosis: effect of Vitamin E. American Heart Journal. 1993, 125: 958-973. 10.1016/0002-8703(93)90102-F
Lin CC, Yin MC, Hsu CC, Lin MP: Effect of five cysteine-containing compounds on three lipogenic enzymes in Balb/cA mice consuming a high saturated fat diet. Lipids. 2004, 39: 843-848. 10.1007/s11745-004-1305-4
Rajamohan T, Kurup PA: Lysine: arginine ratio of a protein influences cholesterol metabolism. Part 1-Studies on sesame protein having low lysine: arginine ratio. Indian J Exp Biol. 1997, 35: 1218-1223.
Satchithanandam S, Chanderbhan R, Kharroubi AT, Calvert RJ, Klurfeld D, Tepper SA, Kritchevsky D: Effect of sesame oil on serum and liver lipid profiles in the rat. Int J Vitam Nutr Res. 1996, 66: 386-392.
Visavadiya NP, Narasimhacharya AV: Sesame as a hypocholesteraemic and antioxidant dietary component. Food Chem Toxicol. 2008, 46: 1889-1895. 10.1016/j.fct.2008.01.012
Ivanovski O, Szumilak D, Nguyen-Khoa T, Ruellan N, Phan O, Lacour B, Descamps-Latscha B, Drüeke TB, Massy ZA: The antioxidant N-acetylcysteine prevents accelerated atherosclerosis in uremic apolipoprotein E knockout mice. Kidney Int. 2005, 67: 2288-2294. 10.1111/j.1523-1755.2005.00332.x
Koros C, Papalexi E, Anastasopoulos D, Kittas C, Kitraki E: Effects of AraC treatment on motor coordination and cerebellar cytoarchitecture in the adult rat. A possible protective role of NAC. Neurotoxicology. 2007, 28: 83-92. 10.1016/j.neuro.2006.07.016
Kleiner DE, Brunt EM, Van Natta M, Behling C, Contos MJ, Cummings OW, Ferrell LD, Liu YC, Torbenson MS, Unalp-Arida A, Yeh M, McCullough AJ, Sanyal AJ: Nonalcoholic Steatohepatitis Clinical Research Network. Design and validation of a histological scoring system for nonalcoholic fatty liver disease. Hepatology. 2005, 41: 1313-1321. 10.1002/hep.20701
Sener G, Balkan J, Cevikbaş U, Keyer-Uysal M, Uysal M: Melatonin reduces cholesterol accumulation and prooxidant state induced by high cholesterol diet in the plasma, the liver and probably in the aorta of C57BL/6J mice. J Pineal Res. 2004, 36: 212-216. 10.1111/j.1600-079X.2004.00122.x
Chen W, Matuda K, Nishimura N, Yokogoshi H: The effect of taurine on cholesterol degradation in mice fed a high-cholesterol diet. Life Sci. 2004, 74: 1889-1898. 10.1016/j.lfs.2003.08.041
Li Y, Gilbert TR, Matsumoto AH, Shi W: Effect of aging on fatty streak formation in a diet-induced mouse model of atherosclerosis. J Vasc Res. 2008, 45: 205-210.
George J, Mulkins M, Casey S, Schatzman R, Sigal E, Harats D: The effects of N-6 polyunsaturated fatty acid supplementation on the lipid composition and atherogenesis in mouse models of atherosclerosis. Atherosclerosis. 2000, 150: 285-293. 10.1016/S0021-9150(99)00377-9
Kamisako T, Ogawa H, Yamamoto K: Effect of cholesterol, cholic acid and cholestyramine administration on the intestinal mRNA expressions related to cholesterol and bile acid metabolism in the rat. J Gastroenterol Hepatol. 2007, 22: 1832-1837. 10.1111/j.1440-1746.2007.04910.x
Beigneux A, Hofmann AF, Young SG: Human CYP7A1 deficiency: progress and enigmas. J Clin Invest. 2002, 110: 29-31.
Davi G, Alessandrini P, Mezzetti A, Minotti G, Bucciarelli T, Costantini F, Cipollone F, Bon GB, Ciabattoni G, Patrono C: In vivo formation of 8-Epi-prostaglandin F2 alpha is increased in hypercholesterolemia. Arterioscler Thromb Vasc Biol. 1997, 17: 3230-3235.
Krieger MH, Santos KF, Shishido SM, Wanschel AC, Estrela HF, Santos L, De Oliveira MG, Franchini KG, Spadari-Bratfisch RC, Laurindo FR: Antiatherogenic effects of S-nitroso-N-acetylcysteine in hypercholesterolemic LDL receptor knockout mice. Nitric Oxide. 2006, 14: 12-20. 10.1016/j.niox.2005.07.011
Diniz YS, Rocha KK, Souza GA, Galhardi CM, Ebaid GM, Rodrigues HG, Novelli Filho JL, Cicogna AC, Novelli EL: Effects of N-acetylcysteine on sucrose-rich diet-induced hyperglycaemia, dyslipidemia and oxidative stress in rats. Eur J Pharmacol. 2006, 543: 151-157. 10.1016/j.ejphar.2006.05.039
Souza GA, Ebaid GX, Seiva FR, Rocha KH, Galhardi CM, Mani F, Novelli EL: N-Acetylcysteine an Allium plant compound improves high-sucrose diet-induced obesity and related effects. Evid Based Complement Alternat Med. 2008, doi: 10.1093/ecam/nen070,
Schaffer JE: Lipotoxicity: when tissues overeat. Curr Opin Lipidol. 2003, 14: 281-287. 10.1097/00041433-200306000-00008
Brizzi P, Tonolo G, Carusillo F, Malaguarnera M, Maioli M, Musumeci S: Plasma lipid composition and LDL oxidation. Clin Chem Lab Med. 2003, 41: 56-60. 10.1515/CCLM.2003.010
Lin CC, Yin MC: Effects of cysteine-containing compounds on biosynthesis of triacylglycerol and cholesterol and anti-oxidative protection in liver from mice consuming a high-fat diet. Br J Nutr. 2008, 99: 37-43.
Hu MY, Li YL, Jiang CH, Liu ZQ, Qu SL, Huang YM: Comparison of lycopene and fluvastatin effects on atherosclerosis induced by a high-fat diet in rabbits. Nutrition. 2008, 24: 1030-1038. 10.1016/j.nut.2008.05.006
Wouters K, van Gorp PJ, Bieghs V, Gijbels MJ, Duimel H, Lütjohann D, Kerksiek A, van Kruchten R, Maeda N, Staels B, van Bilsen M, Shiri-Sverdlov R, Hofker MH: Dietary cholesterol, rather than liver steatosis, leads to hepatic inflammation in hyperlipidemic mouse models of nonalcoholic steatohepatitis. Hepatology. 2008, 48: 474-486. 10.1002/hep.22363
Paigen B: Genetics of responsiveness to high-fat and high-cholesterol diets in the mouse. Am J Clin Nutr. 1995, 62: 458S-462S.
Getz GS, Reardon CA: Diet and murine atherosclerosis. Arterioscler Thromb Vasc Biol. 2006, 26: 242-249. 10.1161/01.ATV.0000201071.49029.17
Kang MH, Kawai Y, Naito M, Osawa T: Dietary defatted sesame flour decreases susceptibility to oxidative stress in hypercholesterolemic rabbits. J Nutr. 1999, 129: 1885-1890.
Bhaskaran S, Santanam N, Penumetcha M, Parthasarathy S: Inhibition of atherosclerosis in low-density lipoprotein receptor-negative mice by sesame oil. J Med Food. 2006, 9: 487-490. 10.1089/jmf.2006.9.487
Karantonis HC, Antonopoulou S, Perrea DN, Sokolis DP, Theocharis SE, Kavantzas N, Iliopoulos DG, Demopoulos CA: In vivo antiatherogenic properties of olive oil and its constituent lipid classes in hyperlipidemic rabbits. Nutr Metab Cardiovasc Dis. 2006, 16: 174-185. 10.1016/j.numecd.2005.07.003
Acín S, Navarro MA, Carnicer R, Arbonés-Mainar JM, Guzmán MA, Arnal C, Beltrán G, Uceda M, Maeda N, Osada J: Dietary cholesterol suppresses the ability of olive oil to delay the development of atherosclerotic lesions in apolipoprotein E knockout mice. Atherosclerosis. 2005, 18: 17-28. 10.1016/j.atherosclerosis.2005.01.050.
We wish to thank Mrs Kalliopi N. Perrea for her kind assistance in laboratory techniques.
The authors declare that they have no competing interests.
LMK has been involved in experimental design, animal experiments, laboratory analysis, data collection and statistical analysis. GA carried out the histopathological examination. AP has been involved in experimental design and animal experiments. ISV participated in experimental design and performed part of the statistics. DI and TK have been involved in experimental design. DNP participated in experimental design, animal experiments, laboratory analysis and data collection. All of the above helped in drafting the manuscript. All authors read and approved the final manuscript.
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Korou, LM., Agrogiannis, G., Pantopoulou, A. et al. Comparative antilipidemic effect of N-acetylcysteine and sesame oil administration in diet-induced hypercholesterolemic mice. Lipids Health Dis 9, 23 (2010). https://doi.org/10.1186/1476-511X-9-23
- Nitric Oxide
- Cholic Acid
- Chow Diet
- High Cholesterol Diet
- Test Diet

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Ord. Seaman C. A. Fearn
The tablets are numbered with roman numerals: I - XVI. Panels 1-10 carry 198...
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Victim of the Druid Street arch bomb, aged 63.
Founder of Carnaby Street as world centre for men's fashion in the 1960s. Fr...
War Memorial WW1
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12,210 brave WW1 seamen remembered on the monument at Tower Hill.
23,831 brave WW2 seamen remembered on the monument at Tower Hill.

domain: history


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An Illinois McDonald's is so desperate for workers that they are now offering a free iPhone to any employee that sticks around for six months and meets certain job criteria. A viral photo was shared on Twitter showing a big window sign hanging from the Altamont, Illinois Mickey D's, which read "Now hiring. Free iPhone!"
A McDonald's spokesperson told Insider on Tuesday that individual restaurants are each offering different incentives to get people to work for the burger giant.
Last month a McDonald’s franchise owner in Tampa, Florida was so desperate to hire some workers, he was offering $50 to anyone who would at least come in for an interview. [Business Insider]

domain: news


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Market Research Report
Global Markets for Plastics Additives
|Published by||BCC Research||Product code||240733|
|Published||Content info||283 Pages
Delivery time: 1-2 business days
|Global Markets for Plastics Additives|
|Published: March 18, 2020||Content info: 283 Pages||
The global market for plastic additives should grow from $60.7 billion in 2020 to reach $77.7 billion by 2025 at a compound annual growth rate (CAGR) of 5.1% for the period of 2020-2025.
Plasticizers, fillers, impact modifiers, and miscellaneous additives as a segment of the global market for plastics additives should grow from $33.5 billion in 2020 to reach $43.1 billion by 2025 at a CAGR of 5.2% for the period of 2020-2025.
Flame retardants, biocides and antistatic agents as a segment of the global market for plastics additives should grow from $11.1 billion in 2020 to reach $14.3 billion by 2025 at a CAGR of 5.1% for the period of 2020-2025.
The report has been prepared and presented in a simple, easy-to-understand format with numerous tables and charts/figures. The report provides a detailed study of the applications and global and regional markets for all types of plastics additives as well as the plastics additives industry. The report's in-depth look at the consolidation and globalization of the plastics processing industry may allow additive manufacturers and suppliers to establish themselves in all the major regions. Estimated values used are based on manufacturers' total revenues. Projected and forecasted revenue values are in constant U.S. dollars, unadjusted for inflation.
The supply and pricing of raw materials and the basic feedstock of various plastics additives have been fluctuating greatly with the run-up in prices of raw materials and energy. This aspect of the plastics processing industry has also been studied and analyzed in detail.
The environmental/regulatory aspects of different types of plastics additives continue to present concerns as well as opportunities to additive suppliers. The effects of these factors on the supply chain, value and demand for additives have also been studied in detail.


Jim Kenney’s Block Party Inauguration Plans
Good morning, Philadelphia: The city’s covered with a dense fog this morning, so be careful out there. Here’s what you need to know today:
Mayor-elect Jim Kenney’s inauguration will feature his “man of the people” roots. But you’ll have to be the right people to get in.
Instead of an inaugural ball, Kenney will celebrate taking office with a block party, complete with a food truck and live music by students from the Philadelphia High School for Creative and Performing Arts — a very “Jimmy from the block” thing to do, no? “It really is just Jim’s style,” a spokesman tells Newsworks. “I think he would have been uncomfortable with anything that was big or extravagant or required him to wear a tux — quite literally uncomfortable with that.”
On the ritzier side: The block party will be held at the Pennsylvania Convention Center — an invite-only affair limited to 1,500 people. And e
veryone who attends is expected to donate at least $5,000 to the Fund for the School District of Philadelphia. (See below.) The event will be held Jan. 4.
[Update] Kenney spokeswoman Lauren Hitt reaches out by email: “Everyone who attends the block party will not be asked to give $5k. Newsworks is updating their story now to reflect the accurate information. The Fund for the School District is hosting a fundraising reception prior to the block party that Jim will attend – the entry price for that is $5k. The invite to our block party is free and we just ask people to give what they can to the District.”
Speaking of parties: The annual Pennsylvania Society shindig was held in New York this weekend — but with the state budget unfinished, it was slightly more “bashful” than “bash.”
Gov. Tom Wolf skipped the affair, and so did most members of the Pennsylvania General Assembly — apparently aware that it would be unseemly to be seen partying while social service agencies and public schools suffer at the same time the state’s budget impasse continues. The result? “It is more mellow and subdued,” political analyst Terry Madonna told PennLive. Among those who made it: former Govs. Tom Corbett and Tom Ridge.
[Update] A previous version of this story said that John Dougherty was in attendance. Dougherty spokesman Frank Keel says that although Dougherty usually hosts a party, he did not this year and was not in attendance at the Pennsylvania Society.
About that budget: Officials think they might be able finish it this week. No really. Stop laughing.
Yes, we’ve heard it so many times now that the only way we’ll believe it is when Gov. Wolf actually signs a budget that’s been passed by both chambers. But there’s signs from Harrisburg that everybody’s ready to finish this thing and move on already. “We know we’ve got to get a budget passed,” House Minority Leader Frank Dermody told AP. “And we’re looking forward to getting it done this week.” Of course, there’s a lot of work to be done between then and now. Among the issues that must be resolved: Tax funding, liquor reform, pension reform, and school funding. So, you know: The easy stuff.
District Attorney Seth Williams, under fire for continuing to employ three “Porngate” attorneys, has offered a mea culpa.
In an interview with the Inquirer, Williams says he should’ve called on Attorney General Kathleen Kane to release all the emails in the matter, ratter than judging the attorneys based on the hundreds of pages of sexist and racist emails that have already been publicized. “I am personally saying mea culpa,” Williams told the paper. “I needed all the evidence, and I didn’t have it. And we need all of it, to restore the public’s trust across this commonwealth.” Nina Ahmad, president of the Philadelphia chapter of the National Organization for Women, still wants the men fired: “What has been released has been enough to incriminate these men, who have clearly shown their deeply rooted biases,” she said.
Philly is home to the best team in Pop Warner football.
The Northwest Raiders beat the Old Town Gators of Maryland, 34-19 — and did so after being down 19-0 early in the game — to win the championship. “I always felt we were going to win the game because we were a better team than them,” running back Ash Wilson told KYW. Coach Duane Watson was careful to stress, though, that Pop Warner has benefits beyond the playing field. “Pop Warner’s about academics. It’s not just go out and play football — you have to maintain grades,” he said. “They have to have study habits. It strengthens the program. It strengthens the community, because now you’ve got kids not just playing a sport. The education part is in there. The mentoring part is in there.” The game took place Saturday in Orlando, Florida.
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A list of equipment from the 2002 James Bond video-game Nightfire.
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Maria Fernanda Romera was an oct produced. The woman is a lovely and most popular Mexican musician, celebrity, and model. She signed up with a famous adolescent pop music class immediately following working in ads. Anybody can become familiar with just how committed she was. Maria come from the beginning and you will got an increase inside her occupation without the favor. Their efforts establish that hard work takes care of. People begin loving their due to the way she discussions, strolls, and you will attire upwards. This woman is just admired by the boys and also inspires of numerous girls out there. I know when you’re good girl, you are included in this also.

domain: artistic


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3. Flour Riot of 1837
The Flour Riot of 1837 was a one-day food riot in which hungry workers protested rising flour and grain prices. The riot also foreshadowed the Panic of 1837, a financial crisis that heavily impacted the city and the American economy more broadly. President Andrew Jackson had passed an executive order called the Specie Circular, which required payment of land to be in hard money, typically gold or silver. As a result, over 400 banks closed entirely or partially failed, and prices began to rise drastically for products like flour, nearly doubling from around $6 to $12 per barrel.
On February 12, around 5,000 people gathered to protest the price of flour and grain, many poor Irishmen. A speaker of unknown identity supposedly rallied the crowd to protest Hart & Co. to lower the price of flour to its original $8. Workers at Hart & Co. closed all doors and windows except one, which allowed protesters to breach the facility. The mayor was pelted with stones and ice, and after the crowd stormed the warehouse of Herrick and Co., the National Guard was called. About 500 barrels of flour were destroyed, and around 40 people were arrested during the riots. In the end, flour prices did not come down, and the depression continued until 1843.

domain: sociology


To remove the graphics card first odsadenie cooling system, which is mounted to the top. For this you need to Unscrew the three screws holding the fan. Next, unplug the power connector of the fan.
Then remove the eight spring-loaded screws that secure the heat cushion on the processor and graphics card. Doing this point be very careful and cautious. There is not a small probability of damaging the graphics chip or processor chip.
The fault can cause a large amount of dust in the radiator that is behind the fan. This is especially true when you live in the house animals with long hair. So after removing the radiator it is recommended that the radiator fins to blow air under pressure.
In last turn loosen the two screws that secure the MXM-cost.
Before install the new graphics card, you need to remove the old heat-conducting casing, and set it to the appropriate location in the notebook. Need to do this so that the memory chips coincided with the thermal pads on the housing.
Remove the old thermal paste thoroughly. Then apply a thin layer of new. When applying a new layer note that the paste should cover the entire surface evenly.
Now install the heat pipes, keeping extreme caution, and in reverse order, tighten the screws and install the fan.
Advice 2 : How to replace graphics card in laptop
Most of the relatively old laptop has one big problem – weak video card. Given the fact that some laptops have integrated video card to replace it for many becomes an impossible task.
You will need
- - Phillips screwdriver;
- - manual to the motherboard;
- - tweezers.
First find out type of video card installed in your laptop. If you are dealing with an integrated video card, it is wise to establish a new full-fledged graphics card. The manual for the motherboard of the laptop.
If it was not at hand, then open the manufacturer's website for this motherboard model and find its description. Find out whether it is a slot for installation of a full graphics card. Specify the type of the given port (if present).
Purchase any video card. Turn the laptop. Phillips screwdriver, Unscrew all the screws. For disassembly of some models you must remove a large part of the screws, and even those that secure additional lid covering the compartments of RAM and hard drive.
Remove the bottom cover of the laptop. You may have to perform this process you need to disconnect a few wires from the motherboard. Be sure to note the slots to which they were connected.
Remove the old video card (if any). Install in the vacant slot new video card. Assemble the laptop by connecting all the necessary cables and installing the base cover. Turn on the device.
Install driver for new video card. If included with the device did not have driver disk, please visit the official website of the manufacturer of this video card model and download there required software.
If your onboard video card is in working condition, it is likely to be currently active. Restart your laptop and enter to BIOS menu. Disable integrated graphics card.
If this is not possible, then use the program installed for a new graphics card to change the active device.
Before you begin to disassemble the laptop, make sure you have all the tools that are described under "You need".
Use tweezers to connect the remote cables.
Advice 3 : How to install a graphics card in the laptop
Replacement graphics can be carried out both in computers and laptops. Some models of laptop are supported by installing an external graphics card, allowing users to produce improvement or repair of equipment. It is worth noting that not all devices support changing video cards.
You will need
- - screwdriver;
- - thermal paste.
Laptops that have the ability to replace the video card, equipped with a MXM connector. The presence of this slot suggests that the device has a discrete graphics card from a third party that is not soldered to the motherboard. If your laptop is present only integrated graphics card that Intel for example, the replacement procedure is not possible, because such adapters are built into the mboard and cannot be removed without special equipment.
Turn off the laptop and disconnect it from the electrical outlet. Remove the battery from the back of the device with the aid of special valves in the housing. Remove the screws that hold the main cover, using a screwdriver.
Under the hood is all the hardware installed on the computer. It is worth noting that on some models the housing is made of special section to replace the graphics card. Therefore, before removing the cover, carefully review the case for the presence of removable parts, and separate blocks.
To remove the graphics card, you'll need to dismantle the cooler that is mounted to the top of the graphics chip. Unscrew the fastening screws, and then remove the power connector from the slot on the motherboard. Then remove the thermal pad that is also fixed by screws, and set them aside. To remove the device as needed being careful not to damage the fragile graphics chip.
Remove the MXM card, removing the remaining bolts. Then carefully lift the card and pull it from the slot. Remove the thermal cover from the old card and attach it to the new adapter in the same way. This must be done so that the location of the thermal pad of the casing coincides with the position of the memory blocks.
Install the new graphics card into the slot and apply on the GPU thin layer of thermal paste, and then screw the card and install the cooling system of the laptop. It should be noted that thermal grease should be evenly cover the entire chip.
Install thermal pads and attach the cooler. Assemble the back plastic part of the laptop and install the cover. After that you can start the laptop to check the results. GPU-installation completed.
Advice 4 : Is it possible to change the integrated graphics in laptop
Unlike desktop computer systems, laptops are more closed in terms of replacement of peripheral equipment. However, some laptop manufacturers allow users to replace certain parts of the structure.
Video cards in laptops
Each laptop has an integrated graphics adapter, which is responsible for displaying on the device screen. Integrated laptop graphics card cannot be replaced, because it is an integral part of the motherboard. This was done to reduce footprint in the housing space and reducing the weight of the laptop in General. Integrated graphics may also be part of the main processor. Today, these graphics cards have the laptops, are presented in the lower and middle price range.
Laptops medium and high range are supplied with an additional discrete graphics cards, loads that move when you run applications that require higher graphics performance. A. in contrast to integrated, the discrete adapter is a complete removable graphics card, which can be replaced by a newer or similar installed.
Before replacing, determine whether your laptop is a discrete graphics card. Usually the discrete video cards Nvidia or Radeon are marked, depending on the manufacturer of the graphics chip.
The procedure for replacing video cards
Unplug the laptop from the mains and remove the battery using special latches on the case. Then Unscrew the main bottom cover of the laptop using a Philips screwdriver, small size. After removing the cover you will be available the contents of the device.
Locate the video adapter that is closer to the HDMI output on the laptop usually on the left side of the motherboard. The graphics card should go, heat pipe cooling, and the top needs to be installed with a special cooling cooler.
Disassemble the cooling system, which also fastens on screws. Remove the cooler and then carefully remove the thermal pads that can also be fixed with special latches or screws.
Removing the cooling system should be done carefully to avoid damaging the graphics chip.
Unscrew the screws securing the Board and carefully remove the graphics card from the slot. Remove thermal cover and install it on a new graphics card in accordance with the location of the RAM device, which must be located on the edges of the Board.
Apply thermal grease on the graphics chip located in the center of the adapter. Install the graphics card into the slot of the video card and then install back heat pillow and a cooling pipe, and then install the cooler and close the back cover of the laptop. Connect the battery and the AC adapter to the laptop. The installation of the graphics card in the laptop is complete.
Advice 5 : How to replace graphics card on a laptop acer
The graphics performance of your laptop plays a vital role when playing videos and games. If you want to replace it, it is necessary to completely disassemble the laptop. This process usually involves the disassembly of complex parts and the laptop case.
You will need
- - Phillips screwdriver;
- - Flat screwdriver;
- - The instruction on disassembling your laptop model;
- - An antistatic wrist strap.
Turn off the laptop, unplug the power cord and disconnect all external devices. Close the display and turn the laptop. Remove the battery from the laptop. Put on an antistatic bracelet before work to prevent damage to the motherboard during disassembly.
Remove the two screw cover memory module compartment in the lower right corner of the laptop using a Phillips head screwdriver. Remove the cover to access the memory chips. Open the latches on each memory chip. Tilt the chip up at an angle, and pull them out of the nests.
Unscrew the screws of the hard drive Bay. Remove the cover to access the hard disk. Unscrew the single screw holding the hard drive. Gently pull the hard drive and unplug the power cord and data transfer. Remove the hard drive from the compartment.
Disconnect the two cables from the wireless module, which is located next to the hard drive Bay. Unscrew one screw and remove the wireless module.
Locate the optical drive. Use a flat screwdriver to open the latch on the side of the drive. Pull out drive through the side opening.
Remove all Phillips screws on the underside of the laptop, flip the laptop over and open the display panel. Locate the SD card slot. Click on the slot cover to remove it and pull from the socket.
Insert a flat screwdriver into the pad above the keyboard. Carefully remove the lid and turn it over. Disconnect the cable volume from the small circuit Board on the bottom side of the lid. Finally, remove the top cover from the laptop.
Press the latches along the top row of keys of the keyboard manually. Disconnect the keyboard ribbon cable from the motherboard and remove the keyboard.
Disconnect the monitor cable from the graphics card in the upper left corner of the motherboard. Unscrew the hinge screws that hold the display. Disconnect the display panel from the laptop.
Disconnect the touchpad from the motherboard. Unscrew three Phillips screws from the top cover. Carefully lift the top cover to access the motherboard.
Remove the Phillips screws holding the video card. Disconnect the graphics card.
Install a new video card and follow all the steps in reverse order.
It is not necessary to upgrade unless absolutely necessary. In case of breakage it is not necessary to calculate for warranty repair, because the warranty does not provide for disassembly of the laptop. You do everything at your own risk
Read the instructions for disassembling the laptop before starting work so that you will not have any difficulties in the process. If you dismantle the laptop for the first time, you should view the video or to do everything under the guidance of a professional


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Back-to-school purchasing is in full swing, if you are gearing up for a brand new session of middle school, higher school, college, or work. Whatever you’re prepping for, your buying list is absolutely brimming with things like desk gadgets, supplies, and dorm decor, however back-to-school clothing is a class everyone can predict a”requirement,” and one crucial thing on the list needs to be the on-sale POPSUGAR Collegiate Sweater ($37, initially $50), which can be readily available for curvy shapes ($40, initially $54). So cute
With a collegiate sense, this V-neck sweater is a contemporary classic, including a supercomfy fit and two-toned stripes. The ivory and blue color palette makes this inexpensive item incredibly versatile, meaning you can design it with a range of dresses staples for example shorts, shorts, pants, dresses, as well as jeans. That equates to tons of easy-to-wear outfits for course, study sessions, on-campus events, and more. It is the best back-to-school item\!
Ahead, store the sweater you’re guaranteed to have tons of use out of. Plus, we are putting you up with loads of ideas for your new school year together with five easy outfit combos along with unique strategies to style your sweater with every and every one. Style course is in session!

domain: design


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Running a successful business relies on two crucial things. Number one, you can attract the right audience. Number two, they pay you on time. Payment is one of the most significant business issues, as expert lawyers at Accuro Maxwell explain. Indeed, recovering a payment that is due or managing issues related to insolvency cases can be highly stressful for small business owners.
Is there anything you can do that can reduce the risk of customers not paying their invoices? As it happens, there are a few tips and tricks that can make a huge difference when it comes to preserving your cash flow.
Offer Flexible Payment Options
The last thing a customer needs is to face difficulties with payment because the business doesn’t provide enough flexibility. eCommerce platforms especially must prioritize different payment methods. An online shop that only takes credit card payments, for example, could be problematic for customers who don’t have a credit card. It doesn’t necessarily mean they can’t pay for their order. But they need alternative methods such as Paypal or even bank transfers.
Sending Your Invoice on Time
When is the right time to send your bill? Ideally, it will entirely depend on your payment terms. For example, if your business needs to record payment before preparing the order, you want to make sure you send the invoice along with payment options as soon as possible. An example would be an interior designer who agrees on a project with a client but requires a 20% deposit before starting the work. The sooner you send the invoice, the sooner you can expect a payment.
Missing your deadline to send an invoice doesn’t mean you won’t get paid. But, it could force the customer to postpone payment until their finances are ready again.
Choosing the Right Payment Terms
Payment terms refer to the deadline during which the business expects to receive the payment. Sending your invoice on time will affect the deadline. It would be unfair, for instance, to request your customer to pay interest on a late invoice if you failed to issue it on time.
But, essentially, businesses can choose between a variety of standard payment terms such as PIA (payment in advance), Net 7, 10, 15, etc. (payment expected within x days), COD (cash on delivery), or even stage payments (set payments over a predetermined period of time).
It may be worth comparing your chosen payment terms with those typically used in your industry for similar amounts. Indeed, payment terms such as PIA may not be manageable for your customers if they face a hefty bill. So, it could be helpful to arrange your terms to make it easier for your customers to make payments.
Should You Accept Negotiations?
Not every customer will try to negotiate their payment down. However, customers who are already facing tough financial situations may ask you to reconsider the terms or leave the interests off.
Is it worth reducing your payments? Not necessarily. But if a customer is at risk of bankruptcy or contact you via a debt manager expert, negotiating your invoice down may be the only way for you to receive some form of payment.
Too many small businesses face handicapping challenges when customers fail to pay their invoices. While not all issues can be avoided, some may be managed through strategic changes in your payment methods, terms, and invoices.

domain: finance


Rumors about Prince Harry and Meghan Markle's plans for their daughter's baptism have been swirling for some time now. The Duke and Duchess of Sussex welcomed baby Lilibet Diana back in June, however, they have not announced any official christening plans as of now.
“Plans for the baby’s christening have not been finalized,” a spokesperson for Harry and Meghan confirmed to E! News. “As such, any assumptions about what will or will not take place are mere speculation.”
One popular theory about Lilibet's christening is that Harry wants to host the special event at Windsor Castle. The Sussexes lived in Frogmore Cottage on the Windsor grounds during their first year of marriage. Lilibet's big brother, Archie, was christened in the private chapel at Windsor Castle. According to a previous report from the Daily Mail, having Queen Elizabeth in attendance is important to Harry.
Harry reportedly floated the idea of having the christening in Windsor while visiting his home country earlier this year. "Harry told several people that they want to have Lili christened at Windsor, just like her brother," a source told the Daily Mail. As the pandemic continues to make travel challenging, especially with two children in two, Harry and Meghan are "happy to wait until circumstances allow."
While royal watchers would be pleased to see Harry and Meghan reunite with their fellow royals, the Sussexes recently turned down another opportunity to travel back across the pond. The couple's spokesperson informed Us Weekly they will not be in attendance at an upcoming party, hosted by Prince William, in honor of Princess Diana.
Back in July, William and Harry came together amid their ongoing estrangement to unveil a new statue of their late mother in the Sunken Gardens at Kensington Palace. The brothers debuted the statue on what would've been Princess Diana's sixtieth birthday.
The statue unveiling was initially set to be a grand event, however, the ceremony had to be scaled back due to the COVID-19 pandemic. Months later, William is set to host an exclusive gala for the statue's donors, as well as close friends and family of Diana. Elton John is one of the few attendees.
Despite Harry's involvement in the statue's commissioning, he will not be traveling back to the U.K. for the party. Instead, he will be "personally connecting with donors regarding the event.”
Many royal experts seem to doubt that Meghan will ever return to the U.K. after stepping down from her royal duties. Should Meghan really not want to return to England, it definitely seems unlikely that Lilibet's christening will be held at Windsor.


In my summer travels and writings, I recently had an opportunity to see the play “The City of Conversation,” by Anthony Giardina. The play is about family and politics, and how the latter could affect the former. In short, and without diving into the work itself too much, the play is about two strong women, […]
Notes from a Corner of the Country
Bloomberg's billions won't decide the Democratic nomination
Mills' budget gives Republicans a chance to say 'yes'
Why you should reject the campaign against vaccines


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With the fall of Tunisia’s and Egypt’s unpopular, planted leaders as a result of people’s revolutions, the Middle East and some other countries are experiencing pangs of democracy that had been suppressed with brutal hands supported by diabolic conspiracies for decades together. In Libya and Bahrain protests are taking severe turn. In Libya, pro-democracy protesters are being dealt with iron fist of Col. Muammar Gaddafi’s troops, who have been asked to suppress the popular revolution with all the might at their command. The multi-level action against the unarmed protesters has claimed over 300 innocent lives. As many as 50 Muslim leaders of Libya have called upon the ruthless administration to stop killing of innocent protesters as in the light of the Qur’ānic teachings taking even one single innocent life is tantamount to killing of the entire human race.
The popular revolution is taking roots in Libya as its fire has spread to all cities and villages in the country, and armed opposition to the protests does not seem to deter the protesters. The situation prevailing therein is turning ever severe with the revolution causing split in the rank and file of the army, sections of which have joined the revolution.
Libya’s ambassador to India, Ali al-Essawi, has also tendered his resignation in protest against his government’s violent crackdown on demonstrators calling for the ouster of Gaddafi. Al-Essawi also accused the government of deploying foreign mercenaries against the protesters.
The deepening chaos in Libya has hit stocks and pushed oil prices sharply higher.With reports suggesting of over 300 people killed, investors are getting increasingly worried about the escalating violence in the country. Unlike Tunisia and Egypt, Libya is a member of OPEC and has a direct impact on global oil production. Under these circumstances, the callous attitude of Gaddafi administration towards the large segments of masses ever flooding the Libyan streets will further harm the national interests of the country.
These popular revolutions spreading over the various countries prove the fact that people’s voice cannot be suppressed however brutal the opposition may be. People now want to experience real democracy on their pulse. The success of popular revolutions in Tunisia and Egypt has given impetus to people elsewhere to follow the suit. It is high time the dictators read the writing on the wall.


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The Los Angeles Rams are rewriting the narrative of their season, defying preseason predictions that labeled them as underdogs. Once anticipated to be the NFL’s worst, the Rams are now on the verge of securing a coveted spot in the playoffs after a compelling 30-22 triumph against the New Orleans Saints on Thursday Night Football at SoFi Stadium.
Preseason Expectations vs. Current Reality:
Preseason projections painted a challenging picture for the Rams, with pundits and oddsmakers foreseeing them as a bottom-tier team, slated to win six games or less. However, the team’s outstanding performance has shattered these expectations, propelling them into playoff contention.
Key Victory Against New Orleans:
In a critical matchup deemed a pseudo-elimination game for both teams, the Rams displayed resilience and determination.
Quarterback Matthew Stafford’s two touchdown passes were instrumental in the 30-22 victory over the Saints, a win that significantly bolsters their playoff chances.
Entering the game with a 52% chance of making the playoffs, the Rams recognized the stakes.
The victory has now elevated their postseason odds to nearly 75%, a remarkable turnaround that defies earlier projections. A loss would have seen those odds plummet below 15%.
The Rams set the tone with a remarkable 14-play, 95-yard drive culminating in a Puka Nacua touchdown pass on fourth down, showcasing their offensive prowess and determination to secure a playoff berth.
As the Rams continue to exceed expectations, their journey to the playoffs becomes increasingly likely. The team’s ability to transform skepticism into success is a testament to their resilience and skill.

domain: game


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The Edgeworth family is well-known in academic circles both in Ireland and abroad. Maria and her father Richard Lovell Edgeworth dominate the knowledge the wider community have about the family, both nationally and internationally. This podcast will concentrate on Françoise Edgeworth and will be accompanied by archive stills and filmed interview that will also work as online and public viewing.
Kitty Hughes, an eminent local historian who has done an in-depth study of Françoise Edgeworth, will be interviewed and the points she raises on this exceptional woman's life will be put into context in the broader late 19th century early 20th century's English and Irish societies. This will give a more rounded and fuller picture of another member of the Edgeworth clan.
Please note that the details of events listed above are provided by the relevant Local Authorities.


This FN FNAR .308 Rifle came in on local trade. It has some patina on the barrel but is not very noticeable. Other than the spots on the barrel, this rifle seems to have been very well taken care of an not abused. It comes with three butt-pads, three cheek combs, 20 rd. magazine, scope rail and package of shims for the stock all in the original box. Buy used and save!
We charge $19.99 to ship handguns and $29.99 to ship long guns.
Trades Accepted: No
Hyatt Gun Shop is America’s largest independently owned gun store and features more than 4,000 guns in stock on any given day. After opening its doors in 1959, Hyatt Gun Shop has been in the same location at 3332 Wilkinson Blvd. and remains a family business to this day with three generations of the Hyatt family working here daily. Why does matter to you? Unlike the big-box stores, the Hyatt family has shared its experience, know how and customer-first attitude that comes with their 50-plus years of being in business.


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Thanks to Mark Mullin for sharing his project with us! He writes:
Movies of software developed to consume 3d sensor data from the Google Tango 3D depth sensing tablet and generate a 3D model from it using voxelization. Two movies of tests run on very nice data, one movie of very nasty^H^H^H^Hoisy data 🙂
Join us every Wednesday night at 8pm ET for Ask an Engineer!
Maker Business — How Authority and Decision-Making Differ Across Cultures
Wearables — Perform operation
Electronics — Soldering Pointer!
Biohacking — Stretchable EEG Temporary Tattoos
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The new fuel cell EV fleet will consist of 50 hydrogen Renault Kangoo vans, which ENGIE Cofely will deploy to service buildings it has under contract in the Ile-de-France.
Here’s the money quote:
The definitive installation will produce hydrogen on site with an electrolyser powered solely by renewable energy. The hydrogen station is designed, manufactured and integrated by McPhy, a leader in production, storage and hydrogen distribution equipment that serves the energy transition.
via TriplePundit Leading Gas Company Launches Hydrogen Fuel Cell EV Fleet


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The 5-year Land Supply is beginning to undermine public trust
Ahead of Rural Planning Week, George Freeman MP explains why he is deeply concerned about the impact of the National Planning Policy Framework and the 5-year Land Supply on villages and towns across rural Britain.
In 2011 we passed the Localism Act. It was a ground-breaking statement of the commitment of a new generation of Conservatives to reverse the tide of centralisation. It recognised that the best way to get the necessary quality and quantity of housing is through suitable neighbourhood plans that allow local councils to set out their own vision and priorities and then require that they deliver on our plan.
But the system isn’t working as it should. Few other issues are as important to my constituents (or to the country if we are to build the homes we need). The abuse of the 5 year land supply was the top local issue in the recent general election campaign. And I promised to come to Parliament and work to find a solution.
This isn’t a new battle. In the 1990s I started the UK’s first ever localism think tank, The Local Identity Agency. Since then I have been a passionate advocate for giving local communities more responsibly for local plans.
But, for those of us who backed localism, the 5 year land supply is beginning to undermine public trust. In fact, it is beginning to foster the very nimbyism that was not there before and compound a distrust in political promises.
Take an example of my own patch. In Mid Norfolk, we have four magnificent towns: Dereham, Wymondham, Attleborough and Watton. Attleborough and Wymondham are both on the A11, just south of Norwich. Norwich is growing very fast. The Norwich research park is booming thanks to the Government’s support through the Industrial Strategy and the support for science and small businesses. In many ways, Norwich is becoming a mini Cambridge, which is only 40 miles down the newly dualled A11. Indeed, when the Government have opened up the Ely junction and made half-hourly the rail service, Norwich will become part of a Greater Cambridge “Cluster”. That is why there is such housing demand along that corridor. There are 15,000-odd houses going in at Ely, 5,000 at Brandon, 5,000 at Thetford, 4,000 at Attleborough and 2,000 at Wymondham. It is a corridor of high growth.
For that reason, my local council wisely suggested that the bulk of its housing target should be placed on that A11 corridor, where the rail and road links support the cluster of development. Unfortunately, the developers recognised that they have those permissions and that allocation there. As a consequence, they have taken the opportunity of the five-year land supply rule to force very substantial, large-scale commuter housing estates on a number of the villages close to Norwich in my constituency. But without the necessary investment in services and infrastructure.
The developers are now piling into south Dereham, along the main roads. The model is all too familiar: large commuter estates dumped along the road without any accompanying infrastructure. A string of villages between Dereham and Norwich—Yaxham, Mattishall and Swanton Morley—have all found themselves the subject of aggressive, large-scale, out-of-town developments.
In each case, the villages have been working on putting together their own village plans, taking the powers that we gave them in the Localism Act. Every time, each local plan has led to more houses being suggested by the local council than were originally envisaged.
Therein lies the game-changing truth at the heart of the Localism Act: if we empower communities to think about their own futures, most will end up planning developments where they want it, in the style they want, in line with their own vision of their own community. People are not naturally “Nimbys”, but they are resistant to growth being dumped on them by a remote bureaucracy, whether it is in Brussels or London.
In Norfolk we could build many more houses if we were able to get the essence of the localism promise right. That would mean building where we want, building how we want, building for local people as well as those moving into the area. And building in a way that supports our grassroots communities. Fundamentally, this means development being seen to be done by and for communities, not to communities by those with no connection or accountability in the community.
Ensuring we get a planning system that works for all is vital for our economy but also for our democracy. At a time when many are asking whether the system works for them, we must prove that it does. To do so, we will have to answer these difficult questions. Addressing the five-year land supply must be top of the list.
George Freeman is the Conservative MP for Mid Norfolk
Matt Thomson, Head of Planning at the Campaign to Protect Rural England has responded to this article:
“A plan led system is essential in delivering the homes that communities need in the right places. With a plan communities have a say in the location and type of development in their area."
His full comment is available here.


October 23, 2012
UPDATE: Insurance commissioner denies homeowners rate increase
By IRENE NOLAN
Carolina Insurance Commissioner Wayne Goodwin has denied a request by
the insurance industry to raise homeowners’ insurance rates by as much
as 30 percent along the coast, including in Dare and Hyde counties.
Goodwin ordered that a hearing be held in the matter of the insurance
companies’ request to raise homeowners’ insurance rates, stating that
the proposed rates appear to be “excessive and unfairly discriminatory.”
hearing, which is open to the public, is scheduled to begin on June 3,
2013, at 10 a.m. at 430 N. Salisbury St. in Raleigh.
will serve as the hearing officer and listen to experts from the
Department of Insurance and the Rate Bureau, which represents the
insurance industry, to decide what rate changes, if any, are warranted.
Oct. 1, the insurance companies requested an overall statewide average
increase of 17.7 percent for homeowners’ insurance rates. However, the
requested increased varied greatly across the state -- from as low as
1.2 percent in Greensboro to 30 percent in the coastal counties.
Opposition to the request was immediate and loud from the coastal counties.
public comment period on the rate filing was held from Oct.3-19 to
engage the public in the ratemaking process. The Department of
Insurance received approximately 8,800 e-mailed or mailed comments, and
approximately 35 people made comments in person during a public comment
session held on Oct. 17.
and other coastal counties passed resolutions opposing the rate
increase, and the Outer Banks Chamber of Commerce wrote a letter to
protest. Dare County manager Bobby Outten was one of the people
who made comments at the public session.
Department of Insurance’s role is to represent the interests of the
public,” said a news release today by the department. “After an initial
review of the filing and comments submitted by the public, department
experts believe the requested rate increases are not justified based on
the data submitted.”
According to the release, the following concerns, among others, may be raised at the hearing:
data: In the ratemaking process, data typically runs two years behind
the date of the rate filing. The filing is based on data from 2005 to
2009; however, data from at least as recently as 2010 was available at
the time this filing was compiled.
factors: The filing includes various risk factors used to calculate the
indicated rate changes. The Rate Bureau claims these factors (such as
the net cost of reinsurance and compensation for assessment risk) are a
necessary cost of doing business in North Carolina. The concern is that
the factors do not appear to be justified and result in a substantial
increase in rates.
methodology: The Rate Bureau uses a methodology that is not allowed in
North Carolina and has been successfully challenged in the 2001 auto
insurance case, which was decided by the N.C. Supreme Court. This
methodology results in excessive profit factors of 10.5 percent.
The Rate Bureau includes a factor for deviations (discounts that some
insurers give some of their policyholders) in the filing that, in
effect, charges discounts back to consumers. The inclusion of a
specific factor for deviations has been previously disallowed numerous
times in auto filings litigated in the N.C. Supreme Court.
model: The hurricane losses are derived using a hurricane model that
does not appear to be adequately documented or justified.
The Rate Bureau filing is available for public review on the Department’s website. To view the entire filing, go to http://pserff.ncdoi.net/pc.html and enter the Serff Tracking Number NCRI-128708881.
The department’s response and the notice of the hearing can be found at http://www.ncdoi.com/media/documents/NoticeOfHearingInTheFilingForRevisedHomeownersInsuranceRates.pdf
Goodwin’s administration, there have been no approved homeowners,
insurance rate increases. The last homeowners’ insurance rate filing
occurred in 2008 when the insurance companies requested a 19.5 percent
statewide average increase. A settlement agreement allowed for a 4.05
percent statewide average increase to go into effect in May 2009.
Rate Bureau can appeal Goodwin’s through the court system, and
companies can raise rates while awaiting an appeals decision. The
difference in the ordered rate and the implemented rate must be held in
escrow. If the Rate Bureau loses its appeal, the escrowed money must be
refunded to policyholders who paid too much.


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prepped and ready to go
I will be teaching a class for our Community College here in town. We will be doing what I have called a 9 Patch with a Twist a k a Disappearing 9 Patch. I have made this one for a few years and have enjoyed it and the ease of making it. Sew when I was asked if I could teach a beginner class this is the one I thought of. I have my own directions but have also included some from Rachel at www.psiquilt.com. She did grant me permission to use it in my hand out for the class and I will have a sample made from it there. Quilters are the best .... thanks Rachel.
Rachel is a busy mom with a lot on the go, but she finds time to quilt , design and share with us. I did participate in quilt along that she had in ......2010 or was it 2009..... the Pinwheel quilt along. I doubled mine up and still want to add some side borders just to make it a little wider.
|Pinwheel quilt along in progress|
Remember to link up and check out the what the others have been up to at The Needle and Thread Network
Take care out there.... today it is freezing out there -30 before any windchill
In stitches and a warm quilt


Foul-mouthed Starbucks customer screams 'I voted for Trump!' at baristas during furious rant while waiting for his coffee
- Jorge de Cárdenas witnessed the incident near the University of Miami
- The man got angry with Starbucks employees when his order was delayed
- He launched a tirade of abuse, shouting that he had voted for Trump
- 'You lost, now give me my money back,' he told a member of staff
A man launched a foul-mouthed tirade at a Starbucks employee, shouting 'I voted for Trump' after his coffee reportedly took too long to arrive.
Jorge de Cárdenas was working at the store near the University of Miami when he witnessed the incident at about 4.30pm on Wednesday.
He claims the disgruntled customers unleashed his fury on an employee behind the counter, who appears to be African-American, calling her 'trash'.
Cárdenas began filming the altercation on his mobile phone, as the man shouted about Trump.
Scroll down for video
A man launched a foul-mouthed tirade of abuse at a Starbucks employee after his order was too slow to arrive
'He said it was "anti-white discrimination",' Cárdenas told Miami New Times, 'so he started verbally abusing the staff.
'One patron called him an a******, so he said, "F*** you, b****". That's when I started filming.'
In the footage, the man shouts: 'I voted for Trump! Trump! You lost, now give me my money back.'
An employee can be heard saying 'congratulations' in a sarcastic tone, before the man turns on her.
'What is your name?' he asks the barista. 'What is your name? I want your card. You are garbage. You are complete trash.'
In footage of the incident, the man shouts: 'I voted for Trump! Trump! You lost, now give me my money back'
Another customer is then heard stepping in, telling the man to stop his behavior.
'Come on,' the customer says. 'There's no need to talk like that. You want to talk like that, take it outside. But do not talk to other people that way.'
Cárdenas says the two men 'almost came to blows,' and in the footage the angry white man threatens to 'punch out' the man who intervened before storming out of the coffee shop with his blond companion.
'The funny thing, besides just the concept of "anti-white discrimination", is just yelling "Trump!" for no reason,' Cárdenas said. 'Like, dude, you won.'
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domain: travel


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The Middle Eastern Society at Edinburgh University organizes a series of talks with Arabic writers.
will be at Blackwell, Edinburgh
on Tuesday 23rd February
Haifa Zangana is a Kurdish-Iraqi novelist and artist based in London who left Baghdad after being persecuted by the Ba’ath regime in the 1970’s. She has published three novels, three collections of short stories, and numerous nonfiction articles. Her first book Dreaming of Baghdad, which is set in 1970’s Iraq has been released in a new edition by The Feminist Press, this year. She also contributed to War with no End, published by Verso.
Blackwell, 53-63 South Bridge, Edinburgh, EH1 1YS.
For further information, please call 0131 622 8222.
Fee entrance is £1.50.

domain: library


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Knoxville, Tennessee's the Dirty Guv'nahs have a classic Southern rock feel that sounds a lot like the Black Crowes, or maybe the Rolling Stones, if the Stones had stopped dead at Exile on Main St. That throwback Southern sound, which the Guv'nahs do really well, is both a blessing and a curse on the band's third album and first national release, Somewhere Beneath These Southern Skies. The best of the songs here, like the joyous opener, "Can You Feel It," the, well, charming "Good Luck Charm," and the solid "Temptation," sound almost frozen out of time, like prized outtakes from some long lost Stones or Black Crowes album project, and with lead singer James Trimble's soulful, emotional vocal approach, they'd fit right in to the set list of either of those earlier bands. Not that the Dirty Guv'nahs are a nostalgia act, or a tribute band, but a kind of facsimile frozen in a bottle out of time in the 21st century, representing and re-creating a classic Southern rock sound without expanding it or taking it anywhere new. Again, that's both good news on Somewhere Beneath These Southern Skies, and it's bad news. The good songs are timeless facsimiles that float out of another era's memory, while the rest of the album's tracks, while well built, recorded, and sung, seem like forgettable, hazy clichés from 30 years ago. The Dirty Guv'nahs are a good band, and they're dynamite live, but perhaps they need to take what they do so well and take a sharp left turn with it into some truly new and original territory. They have the chops to do it.
|Label:||Dualtone Music Group|
Performance CreditsDirty Guv'nahs Primary Artist
Jeff Coffin Saxophone
Mike Haynes Trumpet
Roy Agee Trombone
James Trimble Vocals,Group Member
Aaron Hoskins Drums,Group Member
Chris Doody Organ,Keyboards,Vocals,Group Member
Justin Hoskins Bass,Group Member
Cozmo Holloway Guitar,Group Member
Michael Jenkins Guitar,Group Member
Technical CreditsRichie Biggs Engineer
Jeff Coffin Horn Arrangements
Ross Copperman Producer
James Trimble Composer
Aaron Hoskins Composer
Chris Doody Composer
Justin Hoskins Composer
Michael Jenkins Composer
Shawn Poynter Cover Photo
Scott Murphy Inside Photo


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Anacreon(redirected from Anacreon (poet))
Also found in: Dictionary.
Anacreon(ənăk`rēən, –ŏn), c.570–c.485 B.C., Greek lyric poet, b. Teos in Ionia. He lived at Samos and at Athens, where his patron was Hipparchus. His poetry, graceful and elegant, celebrates the joys of wine and love. Little of his verse survives. Anacreontics, poems in the style of Anacreon, were written from Hellenistic to late Byzantine times.
Born about 570 B.C.; died about 487 B.C. Ancient Greek poet.
The basic motifs in Anacreon’s lyric poetry, of which only small fragments have been preserved, are sensual love, wine, and a carefree life. Poems of this style later became known as Anacreontic poems. A. S. Pushkin, L. A. Mei, and others translated Anacreon into Russian.
WORKS[“Fragments.”] In Poetae melici graeci. Edited by D. Page. Oxford, 1962.
In Russian translation:
Anakreont: Pervoe polnoe sobr. ego soch. v perevodakh russkikh pisatelei. Edited by A. Tambovskii. St. Petersburg, .
[“Fragmenty.”] In Grecheskaia epigramma.[Moscow, 1960.]