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🎪Shambles Festival is a single-day music event that features diverse entertainment combining DJ sets and live music. Shambles Festival aims to act as an inclusive community event that showcases arts, culture and local talent with a keen focus on music.
🎟All ticket holders must be 18+ and the artists featured at the festival will predominantly be playing dance music. The festival site will include a 2 stages, food stalls, and a bar. The music stages will be located in big-top tents to provide shelter in the event of rain and music will run throughout the event.
For tickets please: https://www.shamblesfestival.co.uk/tickets.html
COMING TO MELKSHAM PARK THIS YEAR💃 Two dance music stages🎵 25+ Artists🎪 Big top festival tents
🌭 Delicious Food stalls - Veli'cious
🍻 A bar located in a marquee serving a range of drinks
🔊 Top-end sound system systems
🌴 A festival-decorated outdoor seating area
✨ Full venue lighting & visuals including lasers & CO2 cannons
⏰ The event will take place from 12-10 pm. All set times will be released and shared 48 hours before the festival gates open. We strongly advise arriving just before noon to avoid possible queue times. There is only one entrance point. This will also be the Exit Point during and after the Festival. There will also be several Emergency Exits. These will be clearly signposted and only used in the event of an Emergency.


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At obsessory, we often do fashion pieces dedicated to women of a certain age. Your 20s are for graphic tees, crop tops, and disco shoes, while your 30s are for wardrobe staples with investment pieces that rank high on quality. So, it was interesting to come across Jonet Wooten’s FabulouslyChicOver50.com blog, because she managed to pull together trendy pieces with a seasoned take that’s worth taking a cue from.
That’s why we roped in this mature fashionista for her take on clothes that are ageless. These pieces look fabulous on every woman, erasing a birthdate from the equation; this is exactly why they’re evergreen and worth the money.
Over To Jonet
Fashion, no matter what language you speak or which trend you are currently obsessed with, tells the world who you are. Whether you’re a trendsetter or a have a classic taste in style, as we move gracefully into the next chapter of our lives, you realise that the sweet spot lies exactly in the middle. Combine what’s hot with a certified fashion staple, and you’ve got yourself a winner.
So while you should rotate your trendy pieces based on what’s in, the clothes that should occupy prime real estate in your closet are those that never go out of style. Also, spend on your basics—they will serve you well for years, decades even. High street fashion should be reserved for your experimental pieces only.
On that note, here are five fashion must-haves I recommend you always have in your closet, no matter what age, generation, or style decade you’re in:
Oscar De La Renta Arabesque Print Palazzo Pants, £1,638
If you’re in search of a trend that’s flattering and makes a bigger statement about your style than basic jeans, it’s palazzo pants for the win! These bottoms have been around since the late 1960s-early 70s, and they’re not going anywhere anytime soon. Designers stick to the silhouette while playing with the size of the flare. Wide and flowing, or tailored to an A-line, your palazzo pants with a tank top, blouse, ruffled shirt, or even under a jacket-style top will make you stand out from the crowd. Solid colours are more versatile, but feel free to experiment with off-beat shades and prints if you love the attention.
Hallhuber Basic Blazer Sofia, £89
Often mistaken as exclusive to workwear, simply wearing a blazer in a fresher colour can help you make layering a playful activity. Think: A yellow blazer with a white tank top and maybe blue or purple shorts. This, versus the black blazer with pencil skirt and a blouse for work. For a day look, play it basic with denim jeans and a simple t-shirt; and for cocktails, turn up the volume with a lace top and killer pair of pumps. See, how the fashion staple can run the mile? Blazers provide the ultimate creative starting point to put together a show-stopping OOTD!
Rag & Bone Mid-Rise Boyfriend Jeans, £83.25
In case you missed the memo, designers want you to ditch your skinny jeans in 2017. The year is all about anti-fits and of the lot, it’s the boyfriend jeans that promise to trend on for years to come. They rank high on comfort, which even the fashion week front row patrons are beginning to accept should be the priority. Loose, cuffed and comfy, boyfriend jeans are figure friendly to every shape, and can easily transition from laid-back to polished depending on your mood. My personal favourite pairing: Boyfriend jeans worn with a printed top layered with a cool bomber jacket and some trendy shades.
Indah Sail Maxi, £84.85
Not only is the maxi dress the perfect way to survive hot summer months in style, it’s a clever way to camouflage the bits we’d rather keep under wraps (if you know what I mean). Also, you can transition it into Autumn-Winter by way of clever layering, so this fashion basic is definitely trans-seasonal and a complete must have. Don’t be afraid to go beyond florals—graphic geometric and Aztec prints are big this year. You can wear the maxi on its own, or with a denim jacket for texture and contrast, and add some interesting accessories like a layered necklace or choker, a hat or a fedora, sandals or boots, and a straw bag or large leather tote.
Apc Julianne Cotton Trench Coat, £395
Talk about the ultimate classic fashion piece! The trench coat is iconically British and has stood the test of time like no other. This multi-tasking piece is functional as it protects you against the wind and rain, and yet so stylish, it can hold its own even when it’s wrapped and buttoned and your outfit underneath is not visible. The coat has one foot solidly immersed in the statement-making category. Layer your trench over a feminine dress with a sophisticated pair of heels to go the glam route. Or, slay a comfier look with a pair of jeans, loafers and a backpack-style handbag, draping your trench coat over your shoulders for an added layer of cool.
To end I’d like to quote legendary designer Yves Saint Laurent who summed the idea up so beautifully: “Fashions fade, style is eternal”. By investing in a few staples, you’ll always be on trend!
If you loved Jonet Wooten as much as we did, be sure to catch her at FabulouslyChicOver50.com. The 55-year-old is a seasoned beauty and fashion expert with her on YouTube channel too. She’s got tips, tricks, and reviews you can learn a lot from. What’s more, she was just awarded one of the Best Fashion Over 50 Blogs on the Planet by FeedSpot. Woot, woot!

domain: design


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12 x Happy Moments Claret Red Colour Silk Paper Tassels Garland Wedding Decoration
Happy Moments Silk Paper Tassels in Claret/ Red colours. Contains 12 tassels in 2 assorted colour. 2 x 6 sheets with 12 narrow strips. Made of silk paper. Ideal for garlandmaking, Christmas, Party, Wedding Decorations .
Colours: Claret and Red.
Dimensions: 12 cm x 35 cm
Material: Silk Paper
Quantity: 12 Tassels.

domain: artistic


Fotosold is looking for a web developer that can help with expanding our web platform ([url removed, login to view]) with extra functionalities and helping out upgrading old code. It will be a freelance position with an estimate of 20 hours per week for the next three months with the possibility of full employment afterwards. You will start of with your own VPS where you will have the responsibility of building and maintaining our intranet website.
Must be able to:
- Navigate around in the file system of Linux using SSH/Telnet
- Convert PSDs to HTML using bootstrap as CSS framework.
- Work with version control such as GIT.
- Work with APIs. SOAP/REST.
Common security issues when developing web applications.
3+ years of Experience in developing Web solutions.
Good to know:
- Codeignighter (PHP framework)
Ability to work independently and as part of a team.
Communication and interpersonal skills, team-player spirit
Commitment towards results and eagerness to learn
Design is already created:
[url removed, login to view]


text:
Besides his short fiction, including short stories and novellas, Gabriel García Márquez’s fictional work includes full-length novels, such as his masterpiece and best-known novel, Cien años de soledad (1967; One Hundred Years of Solitude, 1970). In addition, during his long career as a journalist, he has written numerous articles, essays, and reports on a variety of topics, particularly relating to Latin American life and politics. Among his nonfiction works is Noticia de un secuestro (1996; News of a Kidnapping, 1997), an account of the nefarious activities of drug lord Pablo Escobar in 1990.
In 1967, Gabriel García Márquez’s highly acclaimed novel One Hundred Years of Solitude appeared and was immediately recognized by critics as a masterpiece of fiction. As a work of high literary quality, this novel was unusual in that it also enjoyed tremendous popular success both in Latin America and in translation throughout the world. This work made García Márquez a major figure—perhaps the major figure—of contemporary Latin American literature.
García Márquez’s work has been praised for bringing literary fiction back into contact with real life in all of its richness. His combination of realism and fantasy known as Magical Realism (realismo mágico) sets the stage for a full spectrum of Latin American characters. His stories focus on basic human concerns, and characters or incidents from one work are often integrated into others, if only with a passing reference.
García Márquez won the Colombian Association of Writers and Artists Award in 1954, for the story “Un dia despues del sabado.” The novel One Hundred Years of Solitude garnered the French Prix de Meilleur Livre Étranger, the Italian Chianciano Award, and the Venezuelan Rómulo Gallego Prize. Awarding him the Nobel Prize in Literature in 1982, the Nobel committee compared the breadth and quality of his work to that of such great writers as William Faulkner and Honoré de Balzac. In 1988 García Márquez won the Los Angeles Times Book Award, for El amor en los tiempos del cólera (1985; Love in the Time of Cholera, 1988).
In addition to his novels, Gabriel García Márquez has published short stories, screenplays, and nonfiction works such as essays on cultural and political subjects. Many of his short stories were published originally in newspapers; virtually all of these have been collected in volumes in Spanish. García Márquez’s stories have also appeared in The Atlantic Monthly, Esquire, and The New Yorker. Almost all of his stories are available in English.
After the publication of One Hundred Years of Solitude in 1967, Gabriel García Márquez enjoyed increasing international appeal in the Spanish-speaking world and beyond. The initial reaction to the novel’s Spanish edition, which was first issued in Buenos Aires, was overwhelming: New editions were published at the amazing rate of one per week as the public and critics alike applauded the Colombian masterpiece. Reactions around the world were similar as translations were published: In France, the novel was proclaimed the best foreign book of 1969; in Italy, it was awarded the Chianchiano Prize (1969); and in the United States, it was named one of the twelve best books of the year by The New York Times Book Review (1970). One Hundred Years of Solitude has been translated into more than twenty-seven languages.
The worldwide appeal of García Márquez’s masterpiece is widely acknowledged to have been the single most important factor in the extraordinary growth of interest in the Latin American novel. No novelist of the post-World War II era has had an international influence greater than that of García...
(This entire section contains 238 words.)
Márquez; his use of Magical Realism gave rise to one of the dominant trends in world fiction in the 1970’s and the 1980’s. The winner of numerous literary honors, including the Neustadt International Prize for Literature in 1972, García Márquez was awarded the world’s highest literary accolade, the Nobel Prize in Literature, in 1982.
What are some examples of Gabriel García Márquez’s Magical Realism? How do these examples help shape the text in which they appear?
To what extent do the recurrent themes of his works—love and solitude, nostalgia and dignity, death and destiny—reflect the cultural context in which García Márquez writes? To what extent are these themes more universal?
The General in His Labyrinth and Living to Tell the Tale are both based very strongly on real, historical events, yet incorporate a great number of fictive or imaginative elements. In what ways does the inclusion of the fictional benefit or detract from the reader’s experience and/or understanding of the historical events?
In what ways do the works of García Márquez help to bridge a literary gap between the works of Latin American writers and North American and European writers?
In interviews and elsewhere, García Márquez has alluded to the very personal experiences that are the basis for many of his characters, settings, and plots. What might this pattern suggest about the author’s views on the nature of literature?
How has One Hundred Years of Solitude shaped readers’ and critics’ expectations of Magical Realism, both within García Márquez’s texts and in a broader literary canon?
Bell, Michael. Gabriel García Márquez: Solitude and Solidarity. New York: St. Martin’s Press, 1993. This book explores García Márquez’s works from a number of different perspectives, ranging from comparative literary criticism to political and social critiques. Aso included are commentaries on García Márquez’s styles, including journalism and Magical Realism.
Bell-Villada, Gene H. García Márquez: The Man and His Work. Chapel Hill: University of North Carolina Press, 1990. (See Magill’s Literary Annual review) Includes biographical information on García Márquez, analyses of his major works, an index, and a bibliography.
Bell-Villada, Gene H, ed. Gabriel García Márquez’s “One Hundred Years of Solitude”: A Casebook. New York: Oxford University Press, 2002. A dozen essays on García Márquez’s masterpiece, comprising a wide range of critical approaches.
Bloom, Harold, ed. Gabriel García Márquez. New York: Chelsea House, 1989. Essays by eighteen critics, with an introduction by Bloom, on the fiction of García Márquez. Includes two studies of Chronicle of a Death Foretold, estimates of the influences of Kafka and Faulkner, analyses of narrative stylistics, and inquiries into the author’s types of realism.
Byk, John. “From Fact to Fiction: Gabriel García Márquez and the Short Story.” Mid- American Review 6 (1986): 111-116. Discusses the development of García Márquez’s short fiction from his early imitations of Kafka to his more successful experiments with Magical Realism.
Gerlach, John. “The Logic of Wings: García Márquez, Todorov, and the Endless Resources of Fantasy.” In Bridges to Fantasy, edited by George E. Slusser, Eric S. Rabkin, and Robert Scholes. Carbondale: Southern Illinois University Press, 1982. Argues that the point of view of “A Very Old Man with Enormous Wings” makes readers sympathize with the old man by establishing his superiority over the villagers.
González, Nelly Sfeir de. Bibliographic Guide to Gabriel García Márquez, 1986-1992. Westport, Conn.: Greenwood Press, 1994. An annotated bibliography that includes works by García Márquez, criticism and sources for him, and an index of audio and visual materials related to the author and his works.
Hart, Stephen M. Gabriel García Márquez: “Crónica de una Muerte Anunciada.” London: Grant and Cutler, 1994. A thorough critical guide to Chronicle of a Death Foretold.
McMurray, George R., ed. Critical Essays on Gabriel García Márquez. Boston: G. K. Hall, 1987. A collection of book reviews, articles, and essays covering the full range of García Márquez’s fictional work. Very useful for an introduction to specific novels and collections of short stories. Also includes an introductory overview by the editor and an index.
McNerney, Kathleen. Understanding Gabriel García Márquez. Columbia: University of South Carolina Press, 1989. An overview addressed to students and nonacademic readers. After an introduction on Colombia and a brief biography, the five core chapters explain his works in depth. Chapters 1 through 3 discuss three novels, chapter 4 focuses on his short novels and stories, and chapter 5 reviews the role of journalism in his work. Includes a select, annotated bibliography of critical works and an index.
McQuirk, Bernard, and Richard Cardwell, eds. Gabriel García Márquez: New Readings. Cambridge, England: Cambridge University Press, 1987. A collection of twelve essays in English by different authors reflecting a variety of critical approaches and covering García Márquez’s major novels as well as a selection of his early fiction: No One Writes to the Colonel, Innocent Eréndira, and Chronicle of a Death Foretold. Also includes a translation of García Márquez’s Nobel address and a select bibliography.
Minta, Stephen. García Márquez: Writer of Colombia. New York: Harper and Row, 1987. After a useful first chapter on Colombia, the book traces García Márquez’s life and work. Minta focuses his discussion on the political context of the violencia in No One Writes to the Colonel and In Evil Hour. Includes two chapters on Macondo as García Márquez’s fictional setting and another chapter with individual discussions of The Autumn of the Patriarch, Chronicle of a Death Foretold, and Love in the Time of Cholera. Includes a select bibliography by chapter and an index.
Oberhelman, Harley D. Gabriel Gárcia Márquez: A Study of the Short Fiction. Boston: Twayne, 1991. Argues that García Márquez’s short fiction is almost as important as his novels. Suggests that his stories have the same narrative pattern as his novels. Includes five interviews with García Márquez and essays by four critics.
Solanet, Mariana. García Márquez for Beginners. New York: Writers and Readers, 2001. Part of the “Beginners” series of brief introductions to major writers and their works. Very basic, but a good starting point.
Williams, Raymond L. Gabriel García Márquez. Boston: Twayne, 1984. A good introduction to García Márquez’s works for the beginning student.
Wood, Michael. Gabriel García Márquez: “One Hundred Years of Solitude.” New York: Cambridge University Press, 1990. This book provides much of the background information necessary to understand the history and cultural traditions that inform García Márquez’s writings, including insight into the sociopolitical history of Latin America and biographical information about García Márquez himself.

domain: literature


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Yadira De La Riva to use storytelling to change views on the border, immigration
Bilingual artist and educator Yadira De La Riva grew up with a foot on both sides of the U.S. and Mexico border, and she wants to change the narrative on immigration.
She thinks that can happen through the power of art and storytelling.
“Border issues are often intertwined and complicated,” said De La Riva, a visiting artist from New York who will travel through Arizona and northern Mexico, working with Arizona State University students and border communities to teach theater as a tool for social engagement.
“Trying to build a wall is not only contradictory, but is divisive. As two separate countries, we sometimes need to be reminded how interconnected we are through our families, lifestyle and economies. We must remind U.S. and Mexico of this reality.”
The two-week residency — part of the ASU ongoing “Performance in the Borderlands” series — will run Oct. 16–29. It will feature a series of workshops, lectures, performances and public engagements that bridge the Sonoran Desert.
An initiative of the Herberger Institute for Design and the Arts’ School of Film, Dance and Theatre, “Borderlands” is an annual art series of plays, installations and educational workshops that brings together a collection of local and national artists to focus their talents on borderland issues.
This year’s theme, “Voices of Power,” examines the role of women of color in the arts and social justice. The series kicked off Sept. 13 with a panel of prominent artists discussing their work’s potential to drive social and political change. ASU Now will follow the initiative to document the ways it engages people and the region.

domain: sociology


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Where do chowhounds go for melt in the mouth buttery biscuits, like those in the south? Either restaurant or bakery.
The Meetinghouse (Octavia at Bush) has great biscuits with their dinner, plus you can buy frozen biscuit batter to cook at home.
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domain: food


text:
24 October 2013 17:42 [Source: ICIS news]
HOUSTON (ICIS)--Operations at Citgo's refinery in Lemont, Illinois, have been “signficiantly reduced” following a fire on Wednesday night in the crude unit, the company said on Thursday.
No one was injured in the blaze, the refiner said. Repairs of the unit and an investigation into the cause of the fire are underway, Citgo said.
No timetable has been established as to when the crude unit will come back online, the company said, but some downstream units are still operational.
Besides refining crude oil into gasoline, the Citgo operation in the US Midwest also has a 65,000 tonne/year benzene production line and a 65,000 tonne/year toluene production line, according to ICIS Plants & Projects.
For the latest chemical news, data and analysis that directly impacts your business sign up for a free trial to ICIS news - the breaking online news service for the global chemical industry.
Get the facts and analysis behind the headlines from our market leading weekly magazine: sign up to a free trial to ICIS Chemical Business.
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Asian Chemical Connections

domain: news


Tempe police say a man has been taken into custody after a shooting early Tuesday at a light-rail station.
Police spokesman Sgt. Steve Carbajal says the man is identified as 37-year-old David McKenzie. He was booked into Tempe City Jail Tuesday on charges of aggravated assault and being a prohibited possessor of a firearm.
The situation started just before 4 a.m. when a man was shot. Tempe police say the wounded man has non-life threatening injuries. It's not clear what led to the shooting.
Police found McKenzie at Mill Avenue and Rio Salado Parkway near an apartment building.


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Guitar hero xbox 360 controller on pc - Free idm version 5 18 patch download
Get the latest cheats tips, glitches, guides, downloads, hacks, achievements, unlockables, Easter eggs, FAQs, codes, tricks, hints walkthroughs for Guitar Hero III: Legends of Rock on Xbox 360 ( X360). Guitar hero xbox 360 controller on pc. Xbox 360 Rock Band Guitar Hero wired guitars drums. How To: Use your e- drums as a Guitar Hero controller.
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Is the XBOX360 WIRED ( not wireless! Apr 28, · So I just bought guitar hero for my PC ( game only).
Though not officially supported by Guitar Hero III: Legends of Rock ( PC, PS2, PS3, Wii, X360) PC developer Aspyr Media, the wireless Gibson Les Paul guitar controller designed for use with the Xbox 360 will work on the upcoming PC version of the game as well. The Next Chapter in the Guitar Hero Experience Rock the rhythm, lead, and bass guitar tracks in the follow- up sequel to Guitar Hero, now on Xbox 360. Form your guitar duo and shred riffs cooperatively or go head- to- head in all new multiplayer modes. Find great deals on eBay for guitar hero 2 controller xbox 360.
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domain: game


The downing of Malaysian Airlines flight MH17 over a rebel separatists-held region in the eastern part of the Ukraine Republic is being condemned worldwide. The Russian-encouraged murderers who shot the plane with Russian-made missiles must be exposed, tried and punished.
The notion that the pilots should be faulted for flying over a conflict-ridden land promotes tolerance for barbaric and lawless behavior. We are not yet a world ruled by savages and criminals. That air space is over a part of Ukraine that has been taken over by Russians–mercenary officers, thugs and soldiers disguised as volunteers, in the style of the Soviet Union’s KGB commandos, together with the Ukraine-born ethnic Russians who work and fight to make that part of Ukraine belong to Russia.
The Russian propaganda machine tries to make the world believe that the missiles were fired by Ukraine forces battling the Russian-supplied and supported rebels. This lie is easily proved wrong by the fact that Ukraine has not brought any anti-aircraft weapons to the area of conflict because the rebels have no airplanes. Russian President Vladimir Putin has been actively encouraging the ethnic Russian rebellion against the Ukraine Republic. He has sent mercenaries and gangland-type muscle and killers to Ukraine wreaking havoc, targeting government offices and outposts.
Immediate proof that the missiles were fired by the Russian rebels became public. They and their Russian officers rejoiced over social media that they had hit another Ukrainian military aircraft. The Russian rebels before this had shot down six Ukrainian planes. The rebels are coached by famous ex-KGB officers notorious as experts in this kind of warfare that Russia apparently loves to wage. These officers had served in Chechnya, Latvia and other fronts where their subversion skills earned them laurels from the Russian establishment.
One of these notorious Russian officers was heard online making happy noises about the rebels’ latest kill. Alas, the plane they had downed was Malaysia’s MH17.
They pulled down the online posts of their incriminating media celebrations. But they were not quick enough. Recordings of the incriminating audio were made by Ukrainians and even others abroad (amateurs whose hobby is to monitor such social media incidents and, of course, American and European intelligence snoopers).
Malaysia, backed by practically the whole world, except Mr. Putin, is calling for a thorough and zealous investigation.
Mr. Putin cannot abide the present government of Ukraine. It recently signed a comprehensive partnership agreement with the European Union. That is something Putin has long fought against. Before long, Ukraine could become a member of the European Union–and maybe the NATO.
While subverting the independence of Ukraine and making that country fall ruthlessly under the control of Russia is in Mr. Putin’s agenda, he should realize that being involved in the rebellion in eastern Ukraine will only drain a lot of cash, energy and other resources from his country.
The Russian people want to live in the First World lifestyle of the Europeans and the Russian economy is not a marvel of growth and wealth. It cannot afford to spend money that should improve the life of the Russian people for the support of the separatist rebels in Ukraine.
Putin’s policy is the root cause of the MH17 tragedy. May the death of 298 innocent passengers—mostly Europeans and including three Filipinos —make him realize that his dream of resurrecting Holy Russia can be achieved by being a peaceful partner of the United States and the European Union instead of being their enemies.


The Gorilla Locks A2X Smart fingerprint padlock uses both fingerprint and Bluetooth connectivity to provide you keyless security whenever and wherever you need it.
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This is a detailed V8 Crankshaft with a fancy design of balance weights.
I made sure that the loop is big and thick enough so that it can be produced in steel and serve as keychain or in a more precious metal for use as pendant if wished.
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Jio Phone Next | Jio Smartphone Price in India (2021)
JioPhone Next was launched on Thursday at the 44th Annual General Meeting (AGM) of Reliance Industries. The new Jio Phone is developed under the partnership between Reliance Jio and Google that was announced last year.
Recently, Google invested $4.5 billion in Jio Platforms, taking a 7.73 percent stake in the company. This was Google’s first investment under its India Digitization Fund, through which it plans to invest $10 billion over the next five to seven years to accelerate its digital economy.
Jio Phone Next mobile was release on 24 June 2021, and now completely launched in India. The phone comes with a touchscreen display.
As far as the cameras are concerned, the rear camera has been given in Jio Phone Next. Phone Next is based on Android. Jio Phone Next is a dual-SIM (GSM and GSM) mobile that accepts Nano-SIM and Nano-SIM cards too.
Connectivity options on the Jio Phone Next include Wi-Fi, GPS, 3G and 4G (with support for Band 40 used by some LTE networks in India). Sensors of the phone include accelerometer and proximity sensor.
Jio Phone Next Specifications and Price in India
|Release Date||24th June 2021|
|Expected Price||Rs. 5,999 | $79|
|Display||5.5-inch HD display|
|SIM Type||Nano SIM|
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Item no.: B22337 Make: Pontiac Year: 1994 Model(s): GT Type of lit: 11x9 :: 4 page color folder Photos:View 1 Title: A Blast From...The Future - 25th Trans Am Description: Black cover, with yellow and blue shaded lettering, is photograph showing three-quarter frontal view of front half of white 1969 Pontiac Trans Am Hardtop Coupe. Folder opens to 11x17 layout with two page photograph showing three-quarter frontal view of white 1994 Pontiac 25th Anniversary Trans Am GT Hardtop Coupe, with discussion and specifications comparing the two models. Back cover has three photographs showing interior and blue-over-white paint scheme on rear spoiler, with discussion and lists of standard features.
Origin is American :: Copyright 1994.
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use links on left to find complete list of items.

domain: library


Women's Cutter & Buck Weathertec Ridge Full-Zip Jacket
|Price||$86.50||$83.50||$73.91||Call For Pricing||Call For Pricing|
Featuring a zip mock neck to keep the wind away, the Cutter & Buck Ladies' WeatherTec™ Ridge fleece pullover features contrast detailing at the shoulders and back for a modern style. The full-zip style features piecing at the sides for breathability and offers utility with concealed inside right-hand zip pocket for storage. Signature Cutter & Buck pennant embroidery at the back neck. Ridge items are treated with a DuPont Teflon finish that provides water-and-stain-resistant protection allowing you to be ready for anything the weather might bring. Additional features include piped front and back body, self-fabric hem and cuff, and textured hybrid piecing. 100% polyester.
Machine Wash Cold, Normal / Do Not Bleach / Tumble Dry Low, Normal / Low Iron as Needed
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Details of Matchbox 22B 1953 Vauxhall Cresta PA
The Matchbox 1953 Vauxhall Cresta PA. The Vauxhall that this model was based on was in production from 1957 until 1962. It's wrap around windows were particularly distinctive. There are a large number of variations of this model. It was available in pink, cream,copper, gold, pink & blue/green, white finish. Released in 1958 withdrawn in 1965.
Full size picture of Matchbox 22B
close Picture thanks to Eugene Irniger
Model features:2 tone paint, tow hook
Origin:Made in England
Packaging:cardboard box, Matchbox type B ( A MOKO LESNEY with line drawing ), Matchbox type C ( A LESNEY with line drawing ), Matchbox type D ( MATCHBOX Series arched with colour picture )
Vehicle model:Cresta, PA, Velox
Vehicle type:saloon car
Wheels:grey plastic wheels, metal wheels
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domain: hobby


text:
The webinar takes place following the release of the U.S. Department of Agriculture’s April Crop Production and World Agricultural Supply and Demand Estimates (WASDE) reports. These reports and their implications for the upcoming crop year will be the main topics of the webinar.
“With spring and the weather starting to warm up, the planters are ready to roll,” said James Mintert, professor and director of the Center for Commercial Agriculture. “As farmers plan for the coming season, Michael Langemeier, Nathanael Thompson and I will discuss updated information on supply and demand and the possible implications of the USDA reports for the 2020 crop year. The recent report USDA’s review on prospective plantings surprised almost everyone, and we’ll take a closer look at planting intentions and consider how actual plantings might differ from intentions. We’ll also discuss farm income projections and what’s happening with corn and soybean exports, ethanol demand and more.
Langemeier is a professor and associate director of the Center for Commercial Agriculture and Thompson is an assistant professor in the department of agricultural economics at Purdue. Registration is free on the Commercial Agriculture Center website. After registering, attendees will receive a confirmation email with a link to view the live webinar at the scheduled time. Those who cannot participate live can register to receive a follow-up email after the webinar to download the slides and view the recording.
Writer: Torrie Sheridan, 765-496-6032, [email protected]
Media contact: Sarah Zahn, 765-494-7004, [email protected]
Source: James Mintert, 765-494-7004, [email protected]
Agricultural communications: 765-494-8415;
Maureen Manier, Head of Department, [email protected]

domain: agronomy


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See Eau Claire County Circuit Court location, address, and phone number, and public records search. Courts are legal institutions which resolve disputes between parties and rule on legal cases related to civil, criminal, and administrative matters.
|Chippewa County Circuit Court||Chippewa Falls, WI|
|Dunn County Circuit Judge||Menomonie, WI|
|Pepin County Municipal Court||Durand, WI|
|Trempealeau County Circuit Court||Whitehall, WI|
|Buffalo County Circuit Court||Alma, WI|
|Wabasha County Court||Wabasha, MN|
|Barron County Municipal Court||Barron, WI|

domain: geography


Raising a toast to the stunning you with our sangria inspired colour combination in our handwoven cotton saree. The saree is an embodiment of not just your 9-5 pm hard work but your 5-9 pm resilience. Cheers to you;
OCCASION -Work wear;
DESIGN TYPE - Cotton with hand-dyed colours;
CARE TIP - Hand wash;
BLOUSE COLOUR - Running blouse;
EXTRA NOTE: The beauty of any handwoven creation is characterized by slight irregularities which only showcases and enhances the craftsmanship of our artistic weavers.
The colours you see on the device may slightly vary due to the difference in the individual monitor lighting.
Blouse used here is for representation purpose only


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Channel your inner flower child with these hippy-inspired wooden earrings. Hand carved with trendy style, these dance-ready accessories are perfect for summer festivals and everyday wear. Attain a funky look without sacrificing comfort!
- unique hypoallergenic earrings for regular pierced ears, wooden posts
- earrings are roughly 1-1/4" by 1-1/2", lightweight and comfortable
- wooden tropical OM symbol style


The iPhone 7 and iPhone 7 Plus are smartphones designed, developed, and marketed by Apple Inc. They are the tenth generation of the iPhone. They were announced on September 7, 2016, at the Bill Graham Civic Auditorium in San Francisco by Apple CEO Tim Cook, and were released on September 16, 2016, succeeding the iPhone 6, iPhone 6 Plus, iPhone 6S and iPhone 6S Plus as the flagship devices in the iPhone series. Apple also released the iPhone 7 and iPhone 7 Plus in numerous countries worldwide throughout September and October 2016. They were succeeded as flagship devices by the iPhone 8 and iPhone 8 Plus on September 22, 2017.
The iPhone 7's exterior is similar in shape and volume to the iPhone 6 and iPhone 6S, although the camera bump is bigger on the iPhone 7. Alongside the existing silver, gold, and rose gold colors, the device is offered in new colors of matte black, glossy "jet black", and, for a limited time, red. The "jet black" color is a dark shade, high-gloss black finish.


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DeKalb Schools ends Dunwoody-Chamblee Elementary construction contract after spending $5 millionDeKalb County School District Administration and Instructional Complex on Mountain Industrial Boulevard in Stone Mountain. Photo by Dean Hesse.
This story has been updated.
DeKalb County, GA — New Superintendent Devon Horton revealed in a memo sent out Aug. 2 that his predecessor, interim superintendent Dr. Vasanne Tinsley, had terminated a contract with CGLS Architects and Evergreen Construction to build a new 950-seat elementary school in the Dunwoody-Chamblee area.
The school was approved as a SPLOST V project in 2020.
According to information provided by the district, DeKalb Schools had already spent more than $5 million on the new elementary school before pulling the plug. The project’s future is uncertain.
Tinsley evidently terminated the contract because the district was paying $100,000 per month in contracted fees on the project, but no work was being done.
“This lengthy financial burden was unjustifiable and diverted funds that could have been used to address other educational needs,” Horton said.
District 1 Board of Education member Anna Hill agreed in a statement on her Facebook page that the decision was appropriate. However, Hill took issue with the fact that Tinsley’s administration notified CGLS and Evergreen of the decision on April 14 of this year, but did not notify the board of education members.
Hill stated that the board was not aware of the decision until after Horton started work as superintendent on July 1, despite frequent inquiries.
“I have been asking for months via email and at [Board of Education] meetings about the status of this project.” said Hill.
Tinsley said via phone on Aug. 15 that she would need to research the matter further before making a comment, saying she’s no longer engaged with the day-to-day operations of the school district.
“Thank you for calling me and giving me the awareness,” Tinsley told a reporter.
DCSD’s Legal Affairs department confirmed recently that the district has spent $5.3 million on the project thus far. The district does not consider CGLS Architects and Evergreen Construction to be in breach of contract.
Horton said the district’s warm body count as of Aug. 14 is 80,740, about 1,272 students below projections. The warm body count is the number of students recorded at the very beginning of the school year, but more families often join the district after Labor Day, according to one board member. The district won’t have the final enrollment numbers until October.
In 2016, the county schools had more than 100,000 students. Enrollment declined sharply during the COVID-19 pandemic, dropping by about 5,000 students.
Horton said that the project was originally planned both before the pandemic and before the district’s Comprehensive Master Plan and that the district has experienced unexpected enrollment changes that mean the projections for the new elementary school may not be accurate.
In addition, the Comprehensive Master Plan calls for a more thorough process for evaluating facility capacity needs.
Horton said that before re-starting construction, the district will commission a new enrollment analysis that will inform all decisions about construction projects, including Dunwoody-Chamblee.
Dunwoody-Chamblee Elementary came up at the district’s Audit Committee meeting, as an example of how project delays have cost the district money.
Hill said that she looked forward to seeing the results of the new enrollment analysis, and hastened to emphasize that no final decisions have been made.
“All that has happened is that there is a tentative hold on that project,” Hill said.
Horton stated in his memo that DCSD is committed to being fiscally responsible and accountable to the community.
“We are fully aware of our responsibility to use our resources wisely and ensure that any school construction projects can be adjusted to align with current and projected enrollment needs,” Horton said.
Editor and Publisher Dan Whisenhunt contributed reporting to this story.
Clarification: An earlier version of this story contained information about enrollment counts. That portion of the story has been updated after getting additional context from a school board member.
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Seo Hyun Jin managed to make an impactful comeback with her newest K-drama “Why Her?”.
Actress Seo Hyun Jin recently returned in the SBS law drama “Why Her”, where she made quite the impression towards netizens. The series, which opened with a rating of 6.1% and on the rise, airs every Thursday and Friday, starting June 3rd, 2022.
Alongside the intriguing plot and stunning cinematography, the excellent acting performance of leading actress Seo Hyun Jin has become the talk of town.
In “Why Her”, the actress assumes the role of the talented and ambitious law partner Oh Soo Jae, who has won numerous cases and is the top employee at the TK law firm. However, many people are envious of her, and would do everything to take her down.
However, a tragic incident caused Oh Soo Jae to lose her hard-earned prestige, which led to her taking on the position of a university law professor. Here, she reunited with the male lead Gong Chan, who she helped in the past and is played by actor Hwang In Yeop. The duo then set out to clear the name for Oh Soo Jae, facing various challenges along the way.
While boasting a strong, independent, and arrogant exterior, Oh Soo Jae actually gets hurt easily and has a complicated personality. All of these traits are spectacularly delivered by actress Seo Hyun Jin, who will also turn the show into a fashion runway with her eye-catching outfits.
As a result, the actress became a hot topic immediately after the first episode of “Why Her?”, and is now the most buzz-worthy actor, TV drama category, for the first week of June in Korea.
“Why Her?” is broadcasted every Thursday and Friday on SBS.


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Dassault Systemes 3D Experience Forum 2013: “Taking the Cloud Back to the Enterprise, Not the Other Way Around.”
It’s an interesting exercise to study the evolving vocabulary at tech conference keynotes. About five or six years ago, speakers went out of their way to hail PLM. Today many prefer to keep PLM references to a minimum. Instead, they pepper their talks with “social,” “community,” “apps,” and “cloud.” It’s a reflection of tech consumers’ changing attitude. They want easily deployable, social media-inspired solutions with an always-on connection to the web and a cancel-anytime option. Continue reading
This week’s Siemens PLM Software NX CAE Symposium 2013 at the University of Cincinnati featured more than two dozen presentations, roundtables, and panel discussions, as well as two workshop tracks. Siemens PLM Software NX customers explained how they used the software to simulate everything from tiny 0.015mm cardiovascular stents to huge, ship-based cranes and heavy-lift rockets. While the simulation projects were varied, there was a common theme running throughout the symposium: the need to reduce cycle times via collaboration and system-level engineering. Continue reading
What some claim is the originator of the hosted PLM concept and the latest entrant to the category both took the wraps off new versions of cloud-based PLM, spotlighting what appears to be a discernible shift toward this model of deployment.
On-premise deployments and traditional-style PLM still comprise the lion’s share of the market and user implementations, and likely will for some time. However, interest in cloud-based PLM is definitely picking up, in part because of the overall uptick in demand for Software-as-a-Service (SaaS) business software as well as the fact that there are a growing number of SaaS-based PLM offerings to choose from today.
In addition to the splash made by Autodesk with Autodesk PLM 360 and its expanding family of cloud-based engineering tools, there have been a slew of new cloud-based engineering collaboration platforms and refinements to existing platforms, giving engineering customers a deeper selection of choices. According to research by TechNavio, the PLM market is expected to achieve a 9.7% compound annual growth rate between 2011 and 2015, and cloud-based PLM applications, in particular, are expected to drive much of this growth.
One of the companies expecting to capitalize on this anticipated growth spurt is Arena, which came out with the first cloud PLM platform (it was referred to as hosted or on-demand software back then) more than a decade ago and has been at it ever since. Arena settled on what at the time was a disruptive software delivery model because of its target audience—companies in the electronics sector, which typically rely on contract manufacturers and extended global supply chains while dealing with extremely short product delivery cycles and highly accelerated rates of change. Continue reading
In what some might call a stepping stone to the cloud, PTC has acquired its long-time partner NetIDEAS Inc., which specializes in providing software hosting and services around PLM.
For more than a decade, NetIDEAS has provided secure hosting environments for PTC customers looking to offload the heavy lifting around PLM deployment and day-to-day maintenance. Instead of investing in the server and storage infrastructure to run Windchill PLM on their site (along with the IT personnel to administer the enterprise application), NetIDEAS hosts, administers, and supports Windchill along with providing additional consulting services. Continue reading
Siemens PLM Software is now executing on a foundation it’s been building toward for years — releasing a new series of industry-specific solutions, dubbed Catalysts, that aim to take some of the sting out of PLM implementations.
The Industry Catalyst Series will encompass a portfolio of solutions based on a specific industries’ best practices and culled from Siemens’ collective experience collaborating with customers. The idea behind the Catalysts is to help companies jumpstart PLM deployments and accelerate time to value. Continue reading


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Partial Facial Rejuvenation With Teosyal Dermal Filler
botox in the upper face and some volumising to mid and lower face to lift and sculpt the jawline.
The Easy TCA Chemical Peel
The Easy TCA peel is used to correct hyperpigmentation, smooth and clear the complexion and treat acne. A course of 4 is recommended for significant change to skin health.
Nose Reshaping / Non-surgical Nose Job Using Teostyal
Dermal filler is used to shape the nose , especially to straighten the bridge and lift the tip.
Cheeks And Jawline Rejuvenation Using Dermal Filler
Dermal filler used in the cheeks and jawline to reshape and lift the mid and lower face
Nose Reshaping / Non-surgical Nose Job with Dermal Fillers
Dermal filler can be used to straighten the bridge and lift the tip of the nose.
Teosyal Dermal Filler for Tear Troughs
Teosyal Redensity II is the first injectable filler of its kind that has been developed specifically for the tear trough area to address fine lines, dark circles, and deep hollows under the eyes.
Dermal Filler for Tear Troughs
Teosyal; Redensity II for periorbital hollowing and dark circles. Dark shadows or hollows under the eyes, also known as tear trough defects, as they follow an arc shape starting from the corner of the eye where the tear duct is, tend to be accentuated by a weakening of the orbital septum (a membrane under the skin) and a loss of soft tissue in the cheek area.
Facial Rejuvenation - Botox And Cheek Filler
Non-Surgical Facial Rejuvenation Using Dermal Fillers and Botox. In the upper face we use botox and for the cheek lift and volumising we use Teosyal dermal filler.
Lo[ Filler Before And After With Teosyal
dermal filler lip volumising and shaping using Teosyal. Teosyal® fillers can last up to 9-12 months before they completely dissolve naturally in the body. This makes TEOSYAL® one of the longer-lasting dermal fillers available on the market.
Lip Filler - Before And After
Dermal Filler lip treatment has been widely used to increase the size and volume of lips in women for a number of years, and this is still the most popular use for most dermal fillers.
Juvederm VYCROSS Dermal Filler - Lips - Before And After
dermal filler lip augmentation
Consulting Room is the ONLY online cosmetic clinic search directory, for the UK & Ireland, who check that all clinics listed with us are compliant with relevant regulatory requirements for the provision of cosmetic surgery, medical aesthetic treatments, hair transplant surgery and cosmetic dentistry. To find out what this means, why you can have peace of mind and trust the checks we do for you click here.

domain: health


text:
Kimberly’s life has never been easy. Her three brothers cause her enough trouble by trying to set her up with thugs who refuse to work. At least one good thing came from one of her relationships: her daughter Nevaeh. Swearing off men, she focuses her attention to her daughter, her pride and joy, until billionaire Dale Halloway walks into her life – and refuses to leave.
When Kimberly was little, her father was shot and killed by a crooked white cop. Because of this, her family hates white folks, but that’s not stopping Dale. The white billionaire can’t get Kimberly out of his head and he’s not letting her go that easy. Kimberly struggles to keep her and Dale’s relationship a secret for as long as she can. Her brothers would kill him if they knew he was dating their little sister.
When she needs the help of the billionaire, the jig is up and she has to tell her family about her secret relationship with the handsome, built man who would never allow her to say no to him. Disowned by her family, she must decide whether to continue her love affair or remain loyal to her family.


Discuss modifications to the car, flat, sound system in the car, wheels & tires, paint and body kit, the best workshops. Its all about the CAR modification, low dams, car sound system, alloy wheels & tire, paint and bodywork, workshops.
Friday, January 7, 2011
new body kid cars modification
This blog contain new modification motor and car in Motor @ Mobil, New Modification motor and car, Top modification Latest Blog Posts for New Modification


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In 1759, the Burg (sometimes mentioned as Burch or Burgh) slipped of the ramp of a shipyard in Middelburg, Zeeland. Commissioned by the Chamber of Zeeland of the Dutch East India Company (VOC), the Burgmade several voyages to the East. On three occasions she was to sail via Batavia to Dejima. Her journeys to Dejima were met with great peril, as on two out of three journeys she was heavily damaged by strong gales. She made her final journey in 1772, when she and Margaretha Mariawere to sail from Batavia to Dejima.Nearing the Danjo-gunto Islands*, both vessels were caught in a typhoon. During the storm, the Burg lost among other parts her masts, bowsprit, head and quarter-galleries. She sprung a leak, allowing water to built up in the powder-room and hold (Thunberg 1796, p.10). Fearing for their lives, the crew - including M. Daniel Armenault, new Chief of the Dutch trading post on Dejima - transferred to the Margaretha Maria.Expecting the ship to sink, the Dutch left the unmannedBurgbehind andcontinued their journey to Dejima, Japan.
* Known in most contemporary Dutch reports as the Maximaislands, a phonetic corruption of the actual name of the island's group most prominent island which is named Me Shima in Japanese. This island group was used by sailors to determine their position, knowing that by passing Me Shima, they were finally in Japanese waters.
The wreck that did not sink...
Meanwhile, the Burg refused to sink, while she slowly drifted towards the shores of Satsuma in South-West Japan. TheBurgwas found by the Japanese and then towed to Nagasaki, where the vessel was carefully examined and dismantled by local authorities. During the meticulous dismantling process, large quantity of forbidden goods were brought to light, including a chest full of ginseng root thatbelonged to Dutch chief Daniel Armenault. Ginseng root at the timewasby no means to be imported into Japan and the Japanese outrage only grew.In response to the smuggling goods found, Japanese authorities increased their already stringent inspections of every incoming Dutch ship in the subsequent years to come.
Eventually, the hull was all that remained of the stripped downBurg. It was later sold in Nagasaki for scrap in 1773.
Built: 1759 in Middelburg, Zeeland (the Netherlands)
Line: Dutch East India Company (VOC)
Length: 150 ft


Mayhem // Atavistic Black Disorder / Kommando // EP Review
In the chaos that was 2020 Mayhem during their Daemon world tour had to halt everything and return home.
Like most bands during the pandemic they didn’t just waste that time doing nothing, so they conjured up the “Atavistic Black Disorder / Kommando – EP”.
Featuring 3 new tracks that were recorded during the Daemon 2019 sessions & four covers by fantastic punk bands that influenced them.
First of all, let’s talk about the three new tracks.
“Voces ab Alta”, “Black Glass Communion” & “Everlasting Dying Flame”. They may be “leftovers” from the album’s writing session but no way do they lack any quality.
Daemon was my favorite black metal album of 2019 & is now seen as one of the band’s best records. These tracks are as furious & unruly as that whole album.
The first track “Voces ab Alta” is pure mayhem. Their signature sound, reverb-soaked tremolo strumming by Teloch & Ghul. A thumping bass tone by Necrobutcher, chaotic & malicious drumming by Hellhammer & all capped off with Attila ear-piercing shrills & cries of anguish.
“Black Glass Communion” has a more traditional or even old-school blacked metal feel, in the vein of Venom or even Celtic Frost.
The song breaks up into different time signatures and keeps the whole track engaging. Just like the Daemon album these tracks have near-perfect production & capture a studio feel but also keep that live feel you get at Mayhem shows.
This track also features what I can only describe as operatic passages sung by Attila, which turn the dial to 11 and add so much to the track & giving it an edge in a mayhem track I haven’t heard in a while. Very exciting stuff!
Now for the last new track “Everlasting Dying Flame”.
For me, this is my favorite of the three. Still rooted in Mayhem’s sound but for me, this song is non-stop. From the very first note, it is punishing and doesn’t give in at all. Reminds me of the more full blasting Behemoth tracks on their last album “I Loved You At Your Darkest”. Pure fucking armageddon. The perfect way to end the new tracks & finish the Daemon sessions in the best way.
Now for the covers! For me, covers can be hit or miss. Some bands do carbon copies of tracks which can be great or a huge letdown. Or what I think is the best way to cover a track is to do it with the band’s sound.
Now that doesn’t mean Mayhem has gone black metal with these tracks, what they have done is brought a real punk and raw aggression so these tracks which are in some cases over 30 years old. Discharge, Dead Kennedys, Rudimentary Peni & Ramones maybe seem the total opposite to Mayhem but if you know the bands’ history well you will know they are true punk heads & that was one of the bands main influences in conjunction with the new way of British metal in the early 80s.
The four covers are top-notch & are a refreshing b side to 3 fantastic black metal tracks.
If Daemon left you wanting more after its release then this will quench your thirst & hopefully will tide us over until Mayhem returns to the studio for a new album in 2023/2024!!
Atavistic Black Disorder / Kommando -EP is out via Century Media on July 9th, 2021
1. Voces Ab Alta (5:41)
2. Black Glass Communion (4:25)
3. Everlasting Dying Flame (5:52)
4. In Defense Of Our Future (Cover Version) (2:14)


1500mw Laser Engraver Printer, NEJE DK-BL 550x550 Pixel High Resolution Art Craft Science Industry High Speed Laser Engraving Cutter Mini USB Carver Machine Bluetooth 4.0 with Protective Glasses
- CARVED SIZE:42 × 42 × 78 mm Carving Space. 550 × 550 high Resolution Print. Engraving Cutting Machine not need other power/driver, only need the USB interface to complete installation
- STABLE PERFORMANCE: Built-in 6000mAh polymer li-ion battery, Provide 4A output, 108 screws and alloy structure, professional power IC and radiator, High power and create anytime anywhere, Can be used offline without cable connect any power.
- WIDE APPLICATION: Can be carved items included: Hard wood, plastic, bamboo, rubber, leather, Paint metal, miniature engraving, cut paper and so on. It used for print, hand tools and industrial processing.
- WIRELESS：It can be connected via Bluetooth, anytime, anywhere, without being limited to the data line, just use the phone to complete your engraving work. Laser Engraving Machine supports Win 7, XP, Win 8, Win 10, IOS 9.0; APP for iPhone\ iPad \ iPod touch and for Android 4.0 and above.
- OFFLINE OPERATION-This Laser Machine supports running without computer, unique need to lend an image and connect Bluetooth/the USB ports to the charger or power bank (not included)
Details: The laser engraver printer machine prints with high power and 550 x 550 pixel high resolution. It can be controlled by windows PC, iPhone, iPad, iPod touch and Android cell phone. And soft material can print out the 3D and the gradient effect! Unique frame, weak light automatic positioning and frame positioning make the image positioning easier! Good at engraving depth and image engraving.
✔ Built-in 6000mAh Li-ion battery, provide 4A output, high power and create anytime anywhere, can be used offline without cable connect any power
✔ 42 x 42 x 78mm carving space
✔ Laser lifetime up to 100000 hours
✔ Materials cutting depth up to 7.8mm
✔ With 108 screws and alloy structure, make it strong, also you can design your structure in the machine
✔ Power cut memory function. If the battery power is insufficient, use a mobile power or a 5V 1A supply, it will continue to carve
✔ Lone time use, NEJE developed the professional power IC and radiator, carving time will last much longer than other laser and more stable Good at image engraving and depth engraving, extremely wide scope of application
✔ Can be carved items included: hardwood, plastic, bamboo, rubber, leather, metal, miniature engraving, cut paper and so on ( not include gold, silver, copper, and iron )
✔ Support Win 7, XP, Win 8, Win 10, iOS 9.0 and above, Android 4.0 and above
1 × Laser engraving machine
1 × Data cable
2 × Wood slice
1 × CD
2 × Gray cardboard
1 × Wood brick
1 × User manual
1 × L wrench
1 × Whiteboard pen
Package Dimensions: 12.0 x 9.0 x 8.5 inches


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A tour of the castles of Transcarpathia offers to touch the unique historical heritage of Transcarpathia, which will surprise you with its bright diversity. You will discover the mysterious principality of Transylvania, which included the surrounding lands.
You should visit one of the most beautiful castles in our country, where the history of Europe took place, and the walls of which will tell true stories of love, valor, nobility and self-sacrifice. You will not only hear ancient legends, touch the beautiful, but also meet amazing people who reproduce the history, beauty and cultural heritage of the Silver Land not in words but in deeds.
– Departure from Uzhgorod.
– Moving through the village of Seredne, where you will learn about the history of the eastern outpost of the Knights Templar, one of the most mysterious castles not only in Transcarpathia, but in all of Ukraine – Serednyansky Castle.
– Excursion to the historical center of Mukachevo (Roman Catholic Cathedral of St. Martin IV century, Greek Catholic Cathedral IX century, City Hall and other cultural and architectural monuments).
– Excursion to the Mukachevo castle Palanok (IX-VIII centuries), which is rightly considered one of the seven architectural wonders of Ukraine.
– Visiting the castle of love – “St. Miklos” (XV century), located in the village of Chinadiyevo.
– Excursion to the romantic hunting castle of the Counts of Schönborn (XIX century). The hunting castle is built in a neo-romantic style that combines Romanesque and Gothic motifs. Incredibly beautiful architectural structure! A walk along the alleys of the ancient county arboretum park to the source of youth. Lake with ducks and swans, aviary with wild animals. There is a special aura of pacification…
– Moving to Uzhgorod.
The cost of an individual tour is calculated depending on the number of people.
Everyone who is inquisitive, not indifferent to history and knows how to appreciate the beautiful, we invite you to visit a tour of the castles of Transcarpathia. There are 12 castles or their remains on the territory of Zakarpattia region – and we will show the best of them.
Excursion to the castles of Transcarpathia takes place every Sunday. Departure from Uzhgorod and Mukachevo.
Uzhhorod is an exceptionally attractive intertwining and center of various European cultures. Here coexist the brightest aromas and tastes, national traditions and religious denominations. Here you can discover the unique charm of ancient architecture and touching mini-monuments. Then, thanks to the diversity of culture and the proximity of other countries, before going on a trip you can visit the cozy cafes and restaurants, where it is recommended to enjoy delicious food and delicious coffee.
Templar Castle in Transcarpathia - Serednyansky Castle, or rather its ruins, in the village of Seredne, which is located near Uzhgorod.
The power and wealth of the ancient monastic order was admired even by European monarchs. After the first crusade, the treasury of knights trying on robes grew steadily. However, not gold, but another treasure of foothill ores - salt, brought the Templars respect and indescribable profits. Such a precious commodity for medieval Europe needed protection. The generous Carpathian region relentlessly attracted conquerors, and its trade routes - thieves and bandits.
Mukachevo - not too big, European city in the style of Krakow or Prague, built in 896 on the river Latorytsia. His patron is St. Martin. Therefore, the national color of the Transcarpathian region is emphasized by Western influences. Here are special historical monuments and amazing architectural structures. The city impresses with its extraordinary planning, stunning cuisine, cozy restaurants and bright streets. In summer, Mukachevo also attracts fans of rafting and rafting on catamarans on the Tisza and Latorica.
St. Miklos Castle in Transcarpathia is a fortress of love forgotten by contemporaries, hiding under the guise of a worn-out guard at the Russian Gate. This mountain pass opened the way for the West to the valley of the Latoritsa River and further - to all the lands of Russia, so it gathered around it stone guards. In the vicinity of Palanka, which was revived in the restoration, the castle in Chinadiyovo may mistakenly seem like a sleepy old man. But what amazing dreams come true in its towers and halls!
After the trip it is best to inhale the aroma of linden and sakura, relax on the embankment of Uzhgorod, walk towards the forest of UzhNU. Ungvar, who celebrated his millennium, gives everyone a sense of uniqueness. Therefore, after visiting the city, the traveler gives him his whole soul forever. And the memories of a unique tour will remain in your memory for a lifetime.

domain: travel


text:
On the centenary of the Bolshevik Revolution next week, the Kremlin will put on a parade.
The parade, however, won't commemorate the revolution.
In fact, according to Russian press reports, Vladimir Lenin's mausoleum will be closed to the public on the big anniversary of the revolution he led.
Instead, the parade on Red Square will commemorate the 76th anniversary of a speech Josef Stalin gave there to Soviet troops heading off to the front during World War II on the 24th anniversary of the Bolshevik seizure of power.
I guess if you do the math, it makes sense. Seventy-six plus twenty-four does in fact equal one hundred.
But these mathematical gymnastics are very revealing.
Vladimir Putin's regime, of course, has been vocal in its opposition to colored revolutions.
And, as I have noted in the past, the first colored revolution wasn't orange and it wasn't rose. It was red.
It didn't happen in Kyiv or in Tbilisi, but in Putin's own hometown.
And for this Kremlin, that memory is ominous.
But the Kremlin's ambivalence about 1917 runs even deeper.
As the always insightful Russian political commentator Maksim Trudolyubov recently pointed out, the Putin regime seeks to celebrate the Soviet Union's imperial legacy without acknowledging its revolutionary origins.
And it aims to laud the imperial glory of Tsarist Russia, not praise its overthrow.
"The revolution is modern Russia's birth certificate," Trudolyubov wrote, "but Russia does not like what it says."
So Lenin's mausoleum will be closed on November 7. And Stalin's speech will be remembered.


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I’m certainly no stranger to baking with peanut butter. It’s one of my favorite ingredients and flavors. If you’ve been following my baking adventures for a while, you are probably well aware of my affinity for it.
White chocolate, on the other hand, is not really on my list of favorites. I like it well enough, but I don’t find myself clamoring to bake with it. Despite that, I find that it pairs well with so many other ingredients that I use it quite a bit to complement other flavors. Here, that idea applies with white chocolate and peanut butter.
The two flavors work wonderfully together. Peanut butter and chocolate have long been a favorite pair. But the extra sweetness of white chocolate paired with peanut butter is pretty magical, too.
Besides the primary flavor pairing, one of my favorite things about these blondies is the hint of cinnamon. It adds a delightful flavor twist that really makes these bars irresistible. If you aren’t a cinnamon fan, you can simply omit it from the recipe. But if you do like it or are even on the fence about adding it to these blondies, I encourage you to try it. It really amps up the flavor.
These blondies have that fantastic crackly outside and soft, chewy inside thing going on. To attain that wonderful texture, be sure not to overbake them. You want to see a few crumbs when you do the toothpick test. They’ll continue baking a bit in the hot pan once they’re removed from the oven.
As much as I love peanut butter and milk or dark chocolate, these Peanut Butter White Chocolate Blondies are a nice little twist on that classic pairing. They’re a bit sweeter but just as delicious!


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MARCO ISLAND, Fla. – Eleven cars were burglarized and three cars were stolen on Marco Island on Sunday.
Marco Island police are investigating the incidents that happened on the following streets: Echo Circle, Bluebird Ave, Columbus Way, Wayne Ave, Yellowbird St, Riverhead Ave, Tahiti Road, and Worthington St.
Officers said it appears as if all the cars were unlocked with keys still inside when the incidents occurred.
A black Taurus 9mm handgun was taken from one of the vehicles that was burglarized.
One of the cars that was stolen was found Sunday morning in St. Petersburg. The other cars that were stolen are a gray Hyundai Santa Fe with FL tag # 637RVA and a red Chevrolet Camaro with black racing stripes and FL tag # PVDG99.
The Marco Island Police Department released images of the thieves and their associated vehicles.
Officers said it’s important residents never leave their keys, valuables or firearms in their vehicles. People should park in well-lit areas, use vehicle alarms and always lock their cars.

domain: law


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Sea lions are carnivorous animals that feed on squids and octopuses. They also eat large amounts of fish, including sardines, anchovies, herring, salmon, pompano and mackerel.
Fish serves as the primary source of food for sea lions. Sea lions also eat crabs and clams. The sea lion's exact diet varies depending on which food sources are abundant. They often hunt near the shore when there’s an adequate amount of prey. However, they move to areas farther from the coast to find more food when necessary, although this increases their risk of getting eaten by whales and sharks.
When food sources are extremely scarce, sea lions may feed on their young to survive. Researchers believe that consuming other sea lions is a natural instinct that helps control the population of the species, particularly in times of insufficient food supplies.
Once sea lions capture their prey, they swallow the food whole without chewing. However, they use their 34 to 38 teeth to tear large parts of their prey that are hard to swallow. They also use their teeth to break the shells of clams and eat the soft food inside. Sea lions often flip their prey many times until they find the right position to digest food easily.

domain: biology


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Working out can get boring. Summer is the perfect time to try new workout routines.
My problem: I have been working out on my own for over 25 years. I love to work out but it can get so BORING doing the same stuff week in and week out.
My goal: Add something new to my regular workout routines.
My plan: I am all about cross-training with my exercising. Week in, week out, my workout routines are very predictable. I aim to workout six days a week (Sunday is a day of rest!!) Currently, I do a Boot Camp at my gym on Mondays and Wednesdays . A great way to start off the week. Tuesdays are my day to do a 4-6 mile run/walk with my longtime run/walk partner, Ericka. Thursdays I usually do the elliptical in my home gym and Fridays I try to go to a yoga practice. Then there is Saturday. Depending on how much time I have, I will either go on a shorter walk around the neighborhood, try a modified step aerobics video or my favorite, bike rides with my husband.
While my routine is effective, I often reach plateaus so I need to find some new motivation.
Summer is the perfect time to change up workout routines. Longer daylight means more time to get outdoors more. Hiking is fun and different — if you get up early before the heat index goes up. Also, its important to take others along for safety. This laborious hike kicked my butt. I want to continue to add that to my summer workout routines but sometimes its hard to find willing partners that can go with my schedule.
Another good form of summer exercise to change up a workout routine is water aerobics. Why is water aerobics a good choice?
- The buoyancy supports your body so that you can protect your joints, muscles and bones. Being at chest-level 85% to 90% of your body supported.
- Water provides more resistance than air so as you move through the water, you are getting an excellent work out. You will shape up faster doing the same workout in the water than on land.
- Exercising in the water helps improve flexibility and balance. Most people have a wider range of motion in the water.
- And what could be better than being in the water in hot weather?
Lucky for me, my longtime water aerobics instructor recently came back to the area after being gone two years. Carla is an experienced water aerobics instructor. Usually there are 4-6 other women who join in too.
Carla divides the one hour session into legwork and arm work. Since I am not that strong of a swimmer, I get a little nervous and require the assistance of a noodle for some of the exercises. My arms get especially tired. Pushing all that water around provides great resistance. When the hour is over, I am tired yet invigorated, and ready to begin my day.
Change with workout routines is good. I meet new people, develop other muscles, tone different areas and best of all, I am not bored.

domain: sports


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Star of Bethlehem
½ oz doser bottle
This little white flower closes up tightly at night and even during cloudy days. It only opens up, big and bright, when the sun is overhead. The opening and closing is a reflection of how we can hide our pain in the darkness of our own inner strife.
This flower essence helps us to remember and release past stories of grief and trauma. Through remembering the stories, we are able to let go of the built up anger and hurt. It is only through remembering can we truly release. Star of Bethlehem helps us see clearly what traumas from our past are unresolved. It creates a burst of unconditional love through the heart, so we may fully release and move forward. This essence is recommended when doing any type of healing or therapeutic work. When we heal our trauma, we can fully live our purpose.
What is a flower essence?
Flower essences are powerful allies for our emotional bodies. They are energetic imprints of the plant’s vital force and give us an opportunity to connect with the plant on a spiritual level. Flower essences work by getting into the depths of our subconscious and sometimes can create radical changes.
Cognac, Spring Water, and Essence of Star of Bethlehem

domain: psychology


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英文名：Lactose/Sucrose/D-Glucose Assay Kit
规格：115 assays per kit
- Very rapid reaction due to inclusion of galactose mutarotase (patented technology PCT / IE2004 / 00170)
- Very competitive price (cost per test)
- All reagents stable for > 2 years after preparation
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
- Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (firstname.lastname@example.org). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
- State the kit lot number being used (this is found on the outside of the kit box).
- State which assay format was used (refer to the relevant page in the kit booklet if necessary).
- State exact details of any modifications to the standard procedure that is provided by Megazyme.
- State the sample type and describe the sample preparation steps if applicable.
- Sodium borohydride (Sigma S-9125)
- 50 mM NaOH
- 0.2 M acetic acid
- The easiest method is to use a microplate reader that has a path-length conversion capability (i.e. the microplater reader can detect the path-length of each well and convert the individual readings to a 1 cm path-length). This will allow values to be calculated using the MegaCalc calculation software which can be found where the product is located on the Megazyme website.
- Perform a standard curve of the analyte on each microplate that contains test samples and calculate the result of the test samples from the calibration curve (concentration of analyte versus absorbance).
- Perform a standard curve of the analyte in both the cuvette format (i.e. with a 1 cm path-length) and the 96-well microplate format and use these results to obtain a mean conversion factor between the cuvette values and the microplate values. Subsequent assays in the microplate format can then be converted from the calculated conversion factor.
Megazyme低聚半乳糖和半乳糖检测试剂盒采用专利技术，在试剂盒中加入半乳糖变旋酶，可快速催化限速变旋步骤，室温下5分钟即可获得检测结果。试剂盒规格：115次方法：分光光度计，340nm 反应时间： 5分钟检测限： 2.96mg/L 样品类型：牛奶、乳制品（如奶油、奶粉、乳清粉、奶酪、炼乳和酸奶）、含乳食品（如保健食品、焙烤食品、婴幼儿食品、巧克力、糖果和冰淇淋）、食品添加剂、饲料、化妆品、医药及其他物料。方法认证： 通过AOAC、NBN、DIN、GOST以及德国、荷兰、瑞士和奥地利的认证.
The Lactose/Galactose (Rapid) test kit is used for the rapid test of lactose, D-galactose and L-arabinose in food and plant products. Galactose dehydrogenase can be used the measurement and analysis of both D-galactose and L-arabinose. Suitable for the analysis of lactose in “low-lactose” or “lactose-free” samples which contain high levels of monosaccharides.
UV-method for the determination of Lactose and D-Galactose in
foodstuffs, beverages and other materials
(1) Lactose + H2O → β-D-galactose + D-glucose
(2) α-D-Galactose ↔ β-D-galactose
(3) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+
Kit size: 115 assays
Method: Spectrophotometric at 340 nm
Reaction time: ~ 15 min
Detection limit: 2.96 mg/L (lactose)
Milk, dairy products (e.g. cream, milk / whey powder, cheese,
condensed milk and yogurt), foods containing milk (e.g. dietetic foods,
bakery products, baby food, chocolate, sweets and ice-cream), food
additives, feed, cosmetics, pharmaceuticals and other materials
(e.g. biological cultures, samples, etc.)
Methods based on this principle have been accepted by AOAC, NBN,
DIN, GOST and IDF
Q1. Should the pH of the sample be adjusted even for samples in acidic media?
The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.
Q2. Why is the borohydride reduction step required in procedure B?
Procedure B is required for “low-lactose” or “lactose-free” samples containing high levels of monosaccharides. Generally, these types of samples contain high levels of “free” galactose which causes a high background and reduces the dynamic range available to measure the galactose that is released from lactose in the test. To avoid this the borohydride step is used to reduce the free galactose.
Q3. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?
Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.
Q4. There is an issue with the performance of the kit; the results are not as expected.
If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:
Q5. I have a high level of monosaccharides in my sample which is causing a high background level before I measure released monosaccharides. Is there a method to remove the initial monosaccharides and reduce the background level?
Instead of the normal Carrez sample treatment, 1 mL of milk is added to 4 mL of water and 1 mL of 10 mg/mL sodium borohydride (dissolved in 50 mM NaOH and less than 5 hours old). This solution is incubated in a closed plastic container at 40˚C for 30 min, after which it is neutralised by the addition of 2.5 mL of 0.2 M acetic acid, and then simply filtered through Whatman No. 1 filter paper. The extract, that will be hazy, is analysed without any further treatment, and 0.2 mL per assay should be used (according to the normal procedure). Although the samples are all hazy, this haze is stable in the assay and contributes very little to the absorbance.
In the assay the borohydride reduces all reducing sugars in the milk to their sugar alcohols, i.e. glucose goes to sorbitol, galactose goes to galactitol, and the residual lactose that we are interested in goes to lactitol. Then the usual beta-galactosidase in the kit hydrolyses the produced lactitol into galactose and sorbitol. However, as the borohydride has been neutralised at this stage, the galactose remains as galactose, and thus can be acted upon and quantified by the galactose dehydrogenase.
The extra reagents (to K-LACGAR) that are required to perform such analyses are:
Note: After borohydride reduction of the sample the incubation step with beta-galactosidase should be increased to 1 hour.
Q6. Can K-LACGAR be used for the reliable detection of lactose in bakery products at a level of approximately 100 mg per 100g?
Yes, this is possible. Here are two options for sample preparation methods for pastry products:
1. Mill or homogenise sample materials. Weigh out a representative sample and extract with water (heated to 60˚C if necessary). Quantitatively transfer to a volumetric flask and dilute to the mark with distilled water. Mix, filter and use the appropriately diluted, clear solution for the assay.
Alternatively the sample can be treated with Carrez reagents after the extraction with water:
2. Mill or homogenise sample materials. Accurately weigh approx. 1 g of into a 100 mL volumetric flask, add approx. 40 mL of distilled water, mix and store at 60˚C for 15 min with occasional swirling. Add 2 mL of Carrez II solution and mix. Add 2 mL of Carrez I solution and mix. Add 4 mL of 100 mM NaOH solution and mix vigorously. Dilute to volume with distilled water and mix thoroughly. Filter an aliquot of the solution through Whatman No. 1 filter paper.
Discard the first few mL of filtrate. Use the clear filtrate (sample solution) in the assay. Alternatively centrifuge in a microfuge tube at 13000 x rpm and using the clear supernatant in the assay.
The procedures given here can be modified to suit the sample, e.g. the dilution effect of the lactose in the sample can be reduced by extracting the sample in a lower volume of water so that the final concentration of lactose is detectable by the kit. This would need to be assessed by the user.
Q7. The detectable range of lactose is 0.008 – 0.16 g/L using a 1 mL sample but the detection limit is given as 0.00296 g/L for lactose? Why is the ”limit of detection” of your method different from the minimum value of the detectable range?
The linear range of 0.008 – 0.16 g/L is based on the recommended minimum absorbance change of 0.1, however some users are comfortable working below this level hence the limit of detection is based on a 1 mL sample volume and minimum absorbance change of 0.02. The sample volume can be altered; for samples containing low concentrations of lactose the sample volume can be increased to up to 1 mL however the distilled water volume must be altered accordingly so that the final assay volume is not altered, otherwise the calculation will be affected (see page 7 of the K-LACGAR booklet). For concentrated samples these should be diluted in distilled water and the dilution factor included into the calculation.
Q8. Is it possible to measure at a higher wavelength than 340 nm?
It is possible to measure the K-LACGAR reactions at 365 nm. In this instance the extinction coefficient of NADH alters from 6300 [L x mol-1 x cm-1] to 3400 [L x mol-1 x cm-1] and this must be accounted for in the calculation of D-glactose and lactose. The calculations for measurements recorded at 365 nm are shown below.
Note: Alternatively the MegaCalc application may be used for easy processing of raw data values, however if the MegaCalc application is used for calculations recorded at 365 nm then the calculated values (g/L) must be multiplied by 1.8529.
Q9. Can K-LACGAR be used to measure arabinose?
This kit can be used as described in the format below to measure L-arabinose but not D-arabinose:
Wavelength: 340 nm
Cuvette: 1 cm light path (glass or plastic)
Temperature: ~ 25°C
Final Volume: 2.72 mL
Sample solution: 4 – 120 μg of L-arabinose per cuvette
Read against air: without cuvette in light path
* for example with a plastic spatula or by gentle inversion after closing the cuvette with a cuvette cap or Parafilm®.
** if this “creep” rate is greater for the sample than for the blank, extrapolate the absorbances (sample and blank) back to the time of addition of suspension 5.
Determine the absorbance difference (A2-A1) for both blank and sample. Subtract the absorbance difference of the blank from the absorbance difference of the corresponding sample, thereby obtaining DA.
The concentration of arabinose can be calculated as follows:
V = final volume [mL]
MW = molecular weight of arabinose [g/mol]
ε = extinction coefficient of NAD+ at 340 nm
= 6300 [L x mol-1 x cm-1]
d = light path [cm]
v = sample volume [mL]
If the sample has been diluted in addition to the dilution during preparation, the result must also be multiplied by the additional dilution factor, F.
When analysing solid and semi-solid samples which are weighed out for sample preparation, the content (g/100 g) is calculated from the amount weighed as follows:
Q10. How do I know which procedure to use for my sample(s)?
As a general rule: Procedure A is used for samples that are known to contain low levels of free galactose. Procedure B is used for samples that are known to contain high levels of free galactose. If the free galactose content of a sample is unknown it is recommend that the galactose is measured as per the galactose assays in Procedure A.
For “lactose free” samples it is generally recommended that procedure B is used to maximise the absorbance range and enable the sensitive detection of lactose.
“Lactose free” dairy products have usually been processed whereby lactose in the original sample has been hydrolysed to glucose and galactose. These samples will contain high levels of free galactose and should be processed using procedure B. Some samples will be “lactose free” because the original sample never contained lactose so, assuming that the free galactose level is low, these samples can be processed using procedure A.
Q11. Is there a procedure to test solid samples using PROCEDURE B: (For “low-lactose” or “lactose-free” samples containing high levels of monosaccharides)?
Step 1: Add 1 g of sample (or homogenised sample) to 4 mL of water, mix then add 1 mL of 10 mg/mL sodium borohydride (dissolved in 50 mM NaOH and less than 5 hours old). Incubate this solution in a sealed plastic container at 40°C for 30 min then neutralise by the addition of 2.5 mL of 0.2 M acetic acid. Transfer all of the borohydride reduced sample (~ 8.5 mL) to a 10 mL volumetric flask and make the final volume to 10 mL with distilled water. Filter through Whatman No. 1 filter paper or centrifuge in a microfuge at 13000 x g and use the filtrate or supernatant directly in the assay or with an appropriate dilution in distilled water (if required). The filtrate may be hazy but this is stable in the assay and contributes very little to the absorbance. Typically use a sample volume of 0.2 mL in Step 2 of PROCEDURE B.
For analysis of results the dilution is 1 and the concentration of the prepared sample is 100 g/L (i.e. 1 g prepared in 10 mL).
Q12. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?
Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.
Q13. I have some doubts about the appearance/quality of a kit component what should be done?
If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).
Q14. Can the test kit be used to measure biological fluids and what sample preparation method should be used?
The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2
The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.
Q15. Can the manual assay format be scaled down to a 96-well microplate format?
The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.
There a 3 main methods for calculation of results using the microplate format:
Q16. Can the sensitivity of the kit assay be increased?
For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.
Q17. How much sample should be used for the clarification/extraction of my sample?
The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).
Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.
Q18. When using this kit for quantitative analysis what level of accuracy and repeatability can be expected?
The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.
However, the level of accuracy is obviously analyst and sample dependent.
Q19. Can the sensitivity of the kit assay be increased?
Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.
Q20. Must the minimum absorbance change for a sample always be at least 0.1?
No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.


text:
|Add Memory | Add To Friends|
|avaris (profile) wrote, |
on 9-20-2003 at 10:08pm
|Current mood: optimistic
Music: "Ticket to Ride" a la the Beatles
Subject: A word from our sponsor.
|I'm going to try something a little different from the publicly-accepted blog style. Bear with me.
My characters speak for me. I've got a whole cast of them. And because they're mine, a little of myself goes into each. By writing about them...by letting themselves tell their stories...I can express myself, too, in a way.
Now, don't get me wrong. These characters aren't carbon-copies of me. Some things are different; some things don't directly apply to my life. Like Paul's mother. Aidan's sexual preference. Angela's bruised past. Keelin's good luck with love.
Some of them know each other. Some are separate entities. I'll just let them narrate as they go along; if you stick around, you'll begin to understand...maybe you'll even come to know them as I have. And so doing...come to know me a little better, as well.
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