text:
In a historic development, on 1 July 2021, 130 countries agreed to a broad framework for solutions to address tax challenges arising from digitalisation of the economy. Importantly, the solution envisaged does not restrict itself to the digital economy and could substantially reform the present international tax framework. This article is the first in a new series where we track the latest developments with respect to this historic global tax deal and its implications for businesses.
HISTORIC DEAL ACHIEVES NEAR-GLOBAL CONSENSUS ON INTERNATIONAL TAX REFORM
Recently, majority member countries of the OECD/G20 Inclusive Framework on Base Erosion and Profit Shifting (BEPS-IF) agreed to the "Statement on a two-pillar solution to address tax challenges arising from the digitalisation of the economy" (Statement). Out of 139 BEPS-IF member countries, 132 countries (which comprise all G20 countries) have agreed to the Statement as of 14 July 2021. This means that India has also joined this important international tax reform.
At a broad level, the Statement reflects a global agreement on significant changes in the existing rules for allocating taxable income of Multinational Enterprise (MNE) groups between countries, and introduction of new rules to ensure that MNE groups pay a minimum level of tax regardless of where they are headquartered or the jurisdictions they operate in. The Statement is substantially based on the two-pillar solution proposed by the OECD Secretariat (OECD Blueprint) in October 2020, albeit with certain changes compared to the OECD Blueprint.
Traditionally, the taxation of business profits has been linked to a physical presence based nexus rule. While this was sufficient in the brick-and-mortar economy, the rapid digitalisation of the economy exposed gaps in such tax rules. Businesses no longer need physical presence to derive economic benefits from a jurisdiction. This has raised concerns for 'market jurisdictions' having the customer / user base, which, in the absence of a taxing right, are not getting their fair share of tax revenues on the income earned by offshore businesses from their territory. While this issue was first identified around two decades ago and being deliberated on since then, this historic deal marks a concrete step in this direction.
Discussions at a global level to address tax challenges arising from the digitalisation of the economy began with the BEPS project in 2013. Until quite recently, reaching global consensus on the OECD's two-pillar approach to international tax reform seemed unlikely and some countries unilaterally started levying Digital Service Taxes to tax foreign businesses operating in the digital economy. Closer home, India implemented equalisation levy (EL) in 2016 and expanded its scope in 2020 to levy tax on non-residents earning income from India through digital means. However, talks gathered pace in recent months with the Biden administration revitalising the negotiations and lending its weight to the two-pillar solution.
Viewed in that context, the Statement is significant as it marks the first agreement among most BEPS-IF members in arriving at a consensus-based solution on taxation of the digital economy.
A timeline of events leading to the Statement is given below.
Main components of the two-pillar solution
The Statement reflects agreement on changes to tax rules that are clubbed into two 'pillars' – Pillar One and Pillar Two.
As regards Amount A, a final agreement is yet to be achieved on the exact percentage of residual profits that would be allocated within the agreed range of 20% - 30%. Allied with Amount A is the 'tax certainty' component, whereby MNEs will benefit from dispute prevention and resolution mechanisms of a mandatory and binding nature on all issues related to Amount A.
As regards Pillar Two, it should be noted that the GloBE Rules are essentially based on traditional controlled foreign company (CFC) principles that have already been enacted by various countries.
Businesses expected to be impacted by the two-pillar solution
Businesses covered by Pillar One
- Amount A will apply to MNE groups with global turnover above € 20 billion (approximately INR 176,000 crores) and profitability above 10% (ie, profit before tax / revenue greater than 10%) (In-scope MNE groups). It remains unclear whether this turnover threshold will be with reference to only a particular year (for instance turnover for the year 2020), or if it will be tested with reference to every year.
- The Statement provides that 7 years after the agreement comes into force, a review of Amount A's implementation will be conducted; if the review concludes that Amount A has been successfully implemented, the turnover threshold will be reduced to € 10 billion (approximately INR 88,000 crores), bringing more MNEs within the scope of these rules.
- Notably, two sectors are excluded from the scope of Amount A - extractives and regulated financial services.
- While the Statement provides that segmentation will be allowed in exceptional circumstances, at an overall level the Statement appears to suggest that Amount A will apply to all segments of an In-scope MNE group and not just those operating in the 'digital economy'.
Businesses covered by Pillar Two
- The GloBE Rules will apply to MNE groups that are required to undertake country-by-country reporting under applicable transfer pricing regulations, which essentially covers MNE groups having annual consolidated turnover above € 750 million (approximately INR 6,600 crores). The Statement adds that countries are free to apply the minimum tax rate principle to MNEs headquartered in their country even if they do not meet the aforesaid threshold.
- GloBE rules will not apply to government entities, international organisations, non-profit organisations, pension funds or investment funds that are Ultimate Parent Entities (UPE) of an MNE Group or any holding vehicles used by such entities, organisations or funds. Further, GloBE rules shall also provide for an exclusion for international shipping income using the definition of such income under the OECD Model Tax Convention.
Estimated economic impact of the two-pillar solution
Way ahead and manner of implementation
While the Statement indicates agreement among 132 BEPS-IF members on key aspects of the two-pillar solution, an agreement is yet to be reached on certain important issues. This is recognised in the Statement itself, which states that a detailed implementation plan together with remaining issues will be finalised by October 2021.
Also, seven member countries of the BEPS-IF (Ireland, Estonia, Hungary, Nigeria, Kenya, Sri Lanka and Barbados) have not joined the Statement as of date and whether a consensus can be arrived at among all BEPS-IF members would need to be seen. Further, it will be important to keep an eye on whether the US Administration will get US Congress' approval for the two-pillar solution.
The Statement lays down the following timelines and manner of implementation for the two-pillar solution -
- Amount A will be implemented through a multilateral treaty; the Statement provides that it will be developed and opened for signature in 2022, with Amount A coming into effect in 2023. Work on Amount B is expected to be completed by end of 2022.
- The Statement provides that Pillar Two should be brought into law in 2022 and should be effective in 2023. The Statement provides for implementation of Pillar Two proposals in the following manner –
- GloBE Model rules would be implemented by BEPS-IF members through domestic legislation, including the possible development of a multilateral instrument for the purpose of coordinating GloBE Rules implemented by BEPS-IF members. The Statement provides that while BEPS-IF members are not mandatorily required to adopt GloBE Rules in their domestic legislation, BEPS-IF members will be required to accept the application of the GloBE rules applied by other BEPS-IF members.
- For STTR, a model provision together with a multilateral instrument would be developed to facilitate its adoption.
Pillar One – common questions
1. What is the policy rationale behind introducing Amount A?
Under the present international tax treaty framework, cross-border business profits are taxable in the source country provided a taxable presence by way of a Permanent Establishment (PE) exists in the source country. Therefore, income of businesses which operate without any physical presence in market jurisdictions such as technology companies remains largely outside the tax net of such jurisdictions. In response, certain countries have introduced unilateral measures in recent years which bypass the existing tax treaty framework. Such unilateral measures would unlikely be feasible in the long-run due to threats of retaliation by treaty partner countries and dangers of escalation to a trade war, necessitating a consensus-based solution.
Further, developing countries have argued that attribution of profits under the present international tax framework only considers the location of supply factors such as functions, assets and risks. It is contended that the location of demand factors, ie, the consumer, should also be given weightage while attributing profits to a jurisdiction.
To address the above issues, Amount A allocates the taxing rights over certain profits of large MNE groups to market jurisdictions even when there is no physical presence of an MNE group.
2. Will all market jurisdictions be allocated Amount A?
The Statement provides for 'nexus rules' to determine entitlement of a market jurisdiction to an Amount A allocation. The nexus rules essentially provide that –
- Allocation to a market jurisdiction will be permitted when the MNE group derives at least € 1 million (approximately INR 8.8 crores) in revenue from that jurisdiction; and
- For smaller jurisdictions (ie, with GDP lower than € 40 billion (approximately INR 350,000 crores)), the nexus will be set at € 250,000 (approximately INR 2.2 crores).
3. What happens if the residual profits of MNEs are already taxed in a market jurisdiction?
The Statement provides that where the residual profits of an MNE are already taxed in a market jurisdiction on account of marketing and distribution activities in that jurisdiction, the residual profits reallocated to that jurisdiction will be capped by a marketing and distribution profits safe harbour.
4. Will Digital Service Taxes and Amount A co-exist?
The Statement provides that all Digital Service Taxes and other relevant similar measures on all companies will be removed. Therefore, it is possible that India's EL will not be applicable upon implementation of Amount A.
5. How will Amount A work in practice once In-scope MNE groups are identified?
- In respect of In-scope MNE groups, Amount A will be allocated to market jurisdictions in proportion to the revenue derived by the In-scope MNE group from each jurisdiction. It is important to note that profits allocated to market jurisdictions through Amount A will be in addition to existing profits recorded by subsidiaries / PEs located in the market jurisdiction.
- The Statement provides that for determining tax base for allocation of Amount A, profit or loss of the MNE group will be determined by reference to financial accounting income, with a small number of adjustments. Further, losses of the MNE group will be carried forward.
- In order to relieve double taxation burden on MNE groups that may arise on reallocation of profits to market jurisdictions under Amount A, the entity (or entities) that will bear the tax liability will be drawn from those that earn 'residual' profit.
6. Will all constituent entities of an In-scope MNE group be required to undertake filings related to Amount A?
The Statement provides that tax compliance will be streamlined (including filing obligations) and In-scope MNE groups will be allowed to manage the process through a single entity. Further, the 'tax certainty' component of Pillar One is expected to reduce the potential for disputes through setting up of mandatory and binding dispute prevention mechanisms.
Pillar Two – common questions
1. How will the GloBE Rules work in practice?
- The GloBE Rules would comprise of two interlocking domestic rules:
- An Income Inclusion Rule (IIR), which would impose top-up tax on a parent entity in respect of low-taxed income of a constituent entity of the MNE group; and
- To the extent the low tax income of a constituent entity is not subject to tax under an IIR, an Undertaxed Payment Rule (UTPR) would apply and would deny deductions to other entities making payments to such low-taxed constituent entity or require an equivalent adjustment.
- The first step in determining application of GloBE rules would be evaluating whether the combined rate of tax (covered within the scope of GloBE Rules) paid by all constituent entities of an MNE group in a particular jurisdiction is lower than the agreed minimum tax rate. The Statement provides that in the aforesaid evaluation, the tax base would be determined by –
- reference to financial accounting income (with mechanisms to address timing differences and other agreed adjustments); and
- excluding an amount of income that is at least 5% (in the transition period of 5 years, at least 7.5%) of the carrying value of tangible assets and payroll.
- If it is determined that the taxes paid in a jurisdiction are lower than the agreed minimum tax rate, the IIR will allow eligible parent entity (or entities) to impose a 'top-up tax'. If the eligible parent entity (or entities) does not impose a top-up tax on the low-taxed income of a constituent entity, the UTPR will apply to exclude deductions on intra-group payments or require an equivalent adjustment.
2. What is the scope of STTR and how will it work?
- The Statement provides that STTR will allow source jurisdictions to impose limited source taxation on related party payments being interest, royalties and a defined set of other payments that are subject to tax below a minimum rate in the residence jurisdiction. An agreement on the exact scope of 'defined set of other payments' referred in the Statement is yet to be achieved. In this context, it is important to note that the G24 (Group of 24 developing countries which includes India) as well as the African Tax Administration Forum (ATAF) have favoured the inclusion of payments for services and capital gains within the STTR ambit.
- The taxing right of source jurisdictions will be limited to the difference between the agreed minimum rate and the actual tax rate on the payment. The Statement provides that the minimum rate for STTR will be 7.5% - 9%.
India's position on the two-pillar solution
A day after agreeing to the Statement, India's Ministry of Finance issued a Press Release stating that while India is in favour of a consensus solution, the solution should result in allocation of meaningful and sustainable revenue to market jurisdictions, particularly for developing and emerging economies. This was reiterated by India's Finance Minister in her remarks at the recent G20 Finance Ministers and Central Bank Governors meeting.
Notably, the Press Release mentions that some significant issues that remain open and need to be addressed include the share of profit allocation and scope of STTR. Therefore, one can expect that India would negotiate for a final agreement that allocates residual profits on the higher side within the 20% - 30% range agreed for Amount A, and provide for a wider scope of payments that would be subject to STTR.
Way Forward for Businesses
At the outset, MNE Groups should evaluate if they would meet the threshold requirements outlined for Amount A and the GloBE proposal. While the Amount A proposal on profit reallocation is unlikely to impact many MNE groups considering the high thresholds, Pillar Two proposals will cover various MNE groups if the proposed thresholds are adopted.
In the Indian context, Pillar Two proposals are likely to have a significant influence on outbound investment structuring. Therefore, MNE groups should keep these proposals in mind while setting up outbound investment structures over the coming months. Last but not the least, developments in this area must be closely watched considering their significant impact on the global international tax architecture.
The content of this document do not necessarily reflect the views/position of Khaitan & Co but remain solely those of the author(s). For any further queries or follow up please contact Khaitan & Co at email@example.com


text:
North Korea appears to have begun operating a nuclear reactor capable of producing weapons-grade plutonium after a pause of more than 2 1/2 years, according to a new report by the United Nations’ International Atomic Energy Agency.
The 5-megawatt reactor at the Yongbyon Nuclear Research Facility has shown indications of use since last month, according to the IAEA report, which was submitted to the agency’s board of governors on Friday.
“There were no indications of reactor operation from early December 2018 to the beginning of July 2021,” the report said. “However, since early July 2021, there have been indications, including the discharge of cooling water, consistent with the operation of the reactor.”
The nuclear agency has not had access to North Korea since 2009, when Pyongyang expelled its inspectors, and has used satellite imagery to monitor activity at the facility.
The report also detected operations at a steam plant that serves the radiochemical laboratory at Yongbyon from February until July, a time frame it said was “consistent with the time required to reprocess a complete core of irradiated fuel from the 5MW(e) reactor.”
“The new indications of the operation of the 5MW(e) reactor and the Radiochemical Laboratory are deeply troubling,” the report said. “The continuation of the DPRK’s nuclear program is a clear violation of relevant U.N. Security Council resolutions and is deeply regrettable.”
The Democratic People’s Republic of Korea is the official name of North Korea.
The report comes as North Korea has repeatedly lashed out at U.S.-South Korea joint military exercises held this month, calling them a “war rehearsal” and warning of a “security crisis.”
In a statement released Sunday, North Korea’s Foreign Ministry condemned the exercises as “the most vivid expression of the U.S. hostile policy against the DPRK.”
The North will “continuously build up the strongest war deterrent in order to overpower ever-worsening military threats from the U.S.,” the statement said.
Pyongyang has not conducted any nuclear or long-range missile tests since 2017, but it launched a pair of short-range ballistic missiles in March, in violation of United Nations sanctions.
A March report from a U.N. Security Council panel of experts found that North Korea has “not only continued to develop and modernize its ballistic missile program but has also increased its nuclear strike capability.”
The panel assessed that the Yongbyon center is capable of producing around 7 kilograms of plutonium per year and said the country may have a stockpile of 60 kilograms of the radioactive chemical element.
The number of nuclear warheads Pyongyang possesses is uncertain. Last month, the Bulletin of the Atomic Scientists estimated that North Korea has produced enough fissile material to build between 40 and 50 nuclear weapons, but said that it may not have actually assembled that many.
Washington’s efforts at nuclear negotiations with Pyongyang have been stalled since a February 2019 summit between North Korean leader Kim Jong Un and then-U.S. President Donald Trump ended without an agreement.
The Biden administration has signaled a willingness to engage diplomatically with North Korea, taking what it characterizes as a “calibrated and practical approach.”
The top U.S. envoy for North Korea, Sung Kim, visited Seoul last week and reiterated Washington’s desire to engage with Pyongyang.
“I continue to stand ready to meet with my North Korean counterparts anywhere, at any time,” Sung Kim told reporters.
Copyright 2021 United Press International, Inc. (UPI). Any reproduction, republication, redistribution and/or modification of any UPI content is expressly prohibited without UPI’s prior written consent.
This content is published through a licensing agreement with Acquire Media using its NewsEdge technology.

domain: news


text:
Mrs. Langit, Frank, Richard and I went to Senator Fong's Plantation and Garden today. We had a guided tour through the gardens. Our tour guide Patsy was wonderfully informed and made the entire hike very pleasant.
One word of advice...bring mosquito repellent as they will eat you alive. Other than this one caveat the gardens are beautiful and well worth the price of admission. The different areas of the gardens are named after Presidents that Senator Fong served under.

domain: travel


text:
Do not like group trips by boat but still want to visit some of those incredible places and beaches in Crete that are only accessible by boat? Be your own captain! Rent a brand new motor boat for a day or more and explore Mirabello bay hidden beaches & places. No license needed! Minos Boats is a company that rents best boats for recreational reasons, based in Plaka. Fishing, diving, swimming in beautiful waters and discovering beaches, are just a few of the activities that can make your summer vacation unforgettable. Minos Boats is guaranteed to be the best choice in boat rental, offering you safety and quality services.
Your adventure starts right here! The only thing you have to do is to contact us and we will take care of all the rest.


text:
Today we’re joined by Katherine. Katherine is a wonderfully talented artist who does both writing and visual art. She specializes in comics and is currently making a supernatural drama webcomic entitled Soul to Call. She is an incredible storyteller and her work is brimming with an extraordinary amount of detail, as you’ll soon see. My thanks to her for taking the time to participate in this interview.
Please, tell us about your art.
Comics are my specialty, and these days I’m applying those skills to Soul to Call, a supernatural drama webcomic about found family and demons, both inner and outer.
I love writing and drawing equally, so comics are a happy union of those things for me, but I also enjoy just writing or drawing on their own. I write all kinds of fiction, though none of it is currently public beyond my comics, and I enjoy illustrating standalone pieces too! Anything that tells a story, subtle or overt, is my bread and butter.
What inspires you?
Music is a major inspiration for me. It motivates and inspires me every step of the way, from planning, to writing, to drawing. It’s even there for me during artistic blocks. Exercising with some good tunes really gets my brain moving, so if I ever feel stuck or unenthusiastic, walking to music will usually fill my head with new ideas. When I sit back down, I’m rejuvenated and excited to work on my project again.
My friends are also a big source of inspiration to me. I’ve been lucky enough to be surrounded by some wonderful and creative minds. Chats with them leave me inspired to improve myself, and create great work!
What got you interested in your field? Have you always wanted to be an artist?
I’ve wanted to be an artist as long as I can remember, and a comic artist just as long. I’m pretty sure I was drawing and stapling together my own comics since I could hold a pencil. A cliché phrase I know, but I remember drawing comics before I even knew how to spell. I’d give my comics to my mum, then tell her what to write in the speech bubbles I’d left blank. I always made her write more dialogue than could possibly fit in the tiny speech bubble I’d drawn. I’ve gotten a little better at judging the text-to-bubble ratio since then.
I can’t say there was ever a pivotal point in which I got interested in art or comics, it always felt natural to me, and I can’t imagine my life without it. But I guess if I had to credit something for my introduction to comics, it would be my brother reading The Adventures of Tintin, Asterix, and Calvin and Hobbes to me.
Do you have any kind of special or unique signature, symbol, or feature you include in your work that you’d be willing to reveal?
Does texture vomit and tons of purple count? Heheh. I use a lot of textures to give my art a rougher look, and I incorporate my favourite colour purple in anything I can get away with, but otherwise I haven’t committed to a “signature” for my work at this point.
What advice would you give young aspiring artists?
Just go for it! If you have a story or certain kind of art you want to create, don’t wait to be ‘good enough.’ That moment will never come, especially if you just wait around for it. The only way you can gain the skills necessary to make something great is to be making things and honing your craft in the first place! Start creating! You’re gonna make some crap, maybe a lot of crap, but don’t be discouraged, and don’t be afraid to fail! I made two failed webcomics before Soul to Call, but both those failures taught me extremely valuable lessons that lead to Soul to Call’s success.
Make what you want! Create without fear! Don’t be swayed by what you think people want to see. You have a unique vision, and your work will be that much more powerful if you stay faithful to it. And last, but not least, have fun with it. If you’re having fun, eventually people will see it and come have fun with you.
Where on the spectrum do you identify?
Have you encountered any kind of ace prejudice or ignorance in your field? If so, how do you handle it?
I’ve been very lucky to find myself among fellow creative aces, and some wonderfully accepting people in the webcomic community.
Sometimes readers of my comic can be a different story. So far, I haven’t encountered malice, but ignorance over the fact that two of my main characters are on the ace spectrum. Despite some heavy hints in comic, and some blunt statements outside of the comic regarding their orientation, it just doesn’t seem to click for some readers. In most of these cases, I just ignore it, and hope that my writing will speak for itself as I carry on.
What’s the most common misconception about asexuality that you’ve encountered?
I usually encounter the misconception that asexuality is a fancy word for abstinence or celibacy.
I also find a lot of people have trouble wrapping their head around the idea that I can appreciate another person’s appearance, and think they’re exceptionally good looking, without finding them attractive in a sexual way at all. I can appreciate a pretty person the same way someone can appreciate pretty art, folks!
What advice would you give to any asexual individuals out there who might be struggling with their orientation?
You’re not weird, or broken, or sick, and anyone who tells you differently doesn’t deserve your time. Don’t let anyone try to convince you that sex is a be all end all to anything in life. There are so many awesome experiences in the world, and so many ways to be close to other people.
And remember that asexuality is simply a lack of sexual attraction. Is sex something you’re indifferent about? Ace. Is sex is something that repulses you? Still ace. If sex still appeals to you, you just don’t look at people like ‘I wanna bang that,’ that doesn’t invalidate you! Still ace. Don’t let people police you one way or the other. Lack of interest in sexual things doesn’t make you a childish prude, and interest in sexual things doesn’t make you less ace.
Also keep in mind that sexuality is fluid. If you feel ace now, but didn’t before, or don’t in the future, that doesn’t invalidate how you feel now. All our journeys are different. Be kind to yourself, and know there are tons of people out there just like you. You’re not alone.
Finally, where can people find out more about your work?
You can read my comic at soultocall.com
And also find me and my art on a handful of social media like…
Thank you, Katherine, for participating in this interview and this project. It’s very much appreciated.

domain: artistic


text:
TGIF Today for my flashback Friday review I am bringing you a book narrated by the magnificent Joe Arden, Who we were lucky enough to get an interview with… The interview was posted on the blog earlier today and I sure hope you had the opportunity to read it, it was quite divine😍
I listened to this book on audio and it was wonderful! The story is fabulous and it was probably my favorite romance of 2017, so I cannot recommend this book enough! fabulous narration as well as a fantastic love story💕
5 Scrumptious Stars 🌟🌟🌟🌟🌟
Jake McKallister might have been a rock star, but he was no ordinary one. Surviving an unspeakable crime as a young teen had shaped him into a guarded workaholic, and he now lived his life trying to forget. If it hadn’t been for music and the redemption he found through it, he might not have survived. Career success came easily for him. Personal connections did not.
When outspoken, vivacious college student Casey Caldwell was paired with the famously reserved rockstar for a friend’s wedding, she was prepared for the worst. What could they possibly have in common? She was a bubbly talker; he was a reclusive loner. His life was filled with music; she couldn’t carry a tune. She’d enjoyed a happy childhood; his was a well-publicized nightmare.
Yet despite their obvious differences, Jake and Casey found each other, and her light balanced out his darkness. Would their love be strong enough to survive the weight of his tragic past?
I devoured this book just as I would a piece of cake….
Casey was such a fabulous character, she reminded me a little bit of a younger me… always having a little too much to say…. Jake was just a good guy… with a very tragic past, a lot to overcome….
Even though a few things didn’t quite ring true with me( I just can’t imagine a huge rockstar not having more security) this was a very real love story…. I also really appreciated that both characters had a very strong family presence throughout the book….. definitely looking forward to more books from this author, in fact I’ve already started the second book in the series….
PS: the second book as fabulous as well!
🎧🎧🎧5 Smoking Stars for the narration in this book! Wonderful job done by both Joe Arden and Andi Arndt as always!


text:
MoCann Testing Becomes First State-Certified Medicinal Cannabis Testing Lab in Missouri
Original story from Shimadzu
Complete the form below and we will email you a PDF version of " MoCann Testing Becomes First State-Certified Medicinal Cannabis Testing Lab in Missouri"
Shimadzu announces business partner MoCann Testing, a division of EKG Labs, is the first laboratory approved to test medicinal cannabis in Missouri. MoCann Testing offers a full suite of testing services for Missouri Marijuana Cultivators and Manufacturers license holders. Shimadzu is an industry leader in providing analytical instruments, solutions and support that enable fast and easy startup of cannabis and hemp testing facilities.
Their cannabis and hemp testing solutions cover a broad range of QC applications, from highly accurate potency testing and terpene profiling to contaminant testing for pesticides, residual solvents, heavy metals, and mycotoxins/aflatoxins. They also provide platforms and training to ensure experts and novices alike will generate quality results from a wide variety of cannabis samples.
“We are excited to support MoCann’s mission to help individuals across the state of Missouri have access to safe medical marijuana through accurate sample testing and quality results,” said Bob Clifford, Ph.D., general manager of marketing, Shimadzu. “Because selecting the right analytical solutions is a critical investment, we provide more than just advanced instrumentation and software. We assist with method development, instrument training and maintenance that keep systems operating at the highest level.”
“Working with the experts at Shimadzu was key in helping us get our quality analytics and control laboratory up and running quickly,” said Katie Grayson, CEO of MoCann Testing. “We are proud of being the first lab approved by the state to conduct this important testing to safeguard medicinal cannabis products.”
This article has been republished from material provided by Shimadzu. Note: material may have been edited for length and content. For further information, please contact the cited source.

domain: chemistry


text:
Online Job Application for Sport Chek Jobs
As the only big box vendor of sporting goods in Canada, Sport Chek offers a range of athletic apparel. The company sells many popular brands as well as its own line of products.
Sport Chek Job Opportunities
Due to a large demand for workers, shops hire frequently. With more than 130 locations across Canada, the stores regularly hire entry-level employees to serve guests. Hiring managers look for full-time, part-time, and seasonal workers. Job seekers can apply online or submit a Sport Chek application form in-store.
Sport Chek Jobs and Salary Information
An energetic, friendly employee with strong math skills will thrive in this Sport Chek career. Sport Chek is looking for workers to fill these roles:
Key duties for cashiers include:
- Greet patrons and complete purchases.
- This includes handling cash, returns, and customer issues.
- The typical pay per hour is about $11.00.
Job duties for a shipper/receiver include:
- Workers maintain inventory levels in stores and keep the stock room clean and organized.
- These employees must be able to lift at least 22 kg since they load and unload delivery trucks.
- Hourly wages commonly hover around $12.00.
Responsibilities for a sales associate include:
- Employees must guide customers in need of proper attire or equipment.
- Past experience helps but is not required.
- The hourly pay rate is around $11.00.
- Applicants who have a passion for active, healthy living do well in this entry-level Sport Chek job.
Sport Chek Job Benefits
Employment benefits include competitive hourly wages and merchandise discounts. The employers offer flexible work schedules too. Managers try their best to accommodate worker requests. Full-time Sport Chek jobs may earn time off, paid vacation, healthcare coverage, and salary options.
The retailer provides sponsorships to various sports teams. This support isn’t limited to professionals, though. Sport Chek also helps out grassroots organizations and programs that focus on youth development.
How Does Sport Chek Work?
Sport Chek stores do more than just sell sporting goods. Each shop houses specialty boutiques to give advice for specific sports. For example, hockey and golf kiosks cater to shoppers looking to excel at those activities.
Headquartered in Calgary, AB, Sport Chek started in 1999. Originally owned by FGL Sports, the brand grew over the next decade. In 2011, FGL Sports was purchased by Canadian Tire, a retail giant that sells a range of home, hardware, and automotive goods.
What Does Sport Chek Offer its Customers?
The retailer specializes in active wear. Well-trained staff can handle everything from maintenance to repairs on equipment. Employees also offer guidance to shoppers seeking accessories like shoes and clothing. Sport Chek prides itself on creating a one-stop shop for all sports needs.
Canadian Tire, Sport Chek’s parent company, grosses over $12 billion annually. Thanks to a series of investments made since 1994, Canadian Tire has seen much growth and success. The corporation trades on the Toronto Stock Exchange.
Minimum Age to Work at Sport Chek: 15

domain: economics


notify me when available
Please enter your email so we can alert you when the MEN’S THERMOBALL™ TRICLIMATE® JACKET in is back in stock. We promise not to spam you. You will only be notified once.
You will earn 2,990 PeakPoints with this product
Our products are fully warranted against defects in materials and workmanship with a lifetime guarantee. Exclusions apply*. See Details
Returns are easy! VIPeak Members get free online returns. Everyone gets free returns in-store.
FIND IN A STORE
Please enter your "City, State" or "Zip Code" to find stores
STORES - Available as of March 30, 2017 10:40:07 AM EDT
Impenetrable moisture barrier to guarantee dryness.
Insulation traps hot air for reduced heat loss and increased warmth.
Pairs with complementing garments for layering.
- 3-in-1 jacket has a waterproof, breathable DryVent™ 2.5L shell and a removable ThermoBall™-insulated inner jacket.
- [Outer Jacket] Relaxed fit
- Waterproof, breathable, seam-sealed DryVent™ 2.5L shell
- Zip-in-compatible integration with complementing garments from The North Face®
- Attached, fully adjustable hood
- Pit-zip venting
- Exposed, center front zip
- Concealed secure-zip chest and hand pockets
- Velcro® adjustable cuff tabs
- Hem cinch-cord
- Media compatible
- Raised high-definition logos
- Heathered fabric in select colorways
- [Inner Jacket] 10 g/ft² PrimaLoft® ThermoBall™ synthetic insulation throughout the body with 60 g PrimaLoft® Silver Insulation Eco in the sleeves, side panels and collar
- Secure-zip covered hand pockets
- Avg Weight:
- 780 g (27.5 oz)
- Center Back:
- Solid Outer:
- 75D 130 g/m² DryVent™ 2.5L—95% polyester, 5% elastane
- Heather Outer:
- 80D 133 g/m² DryVent™ 2.5L—95% polyester, 5% elastane
- 30D 45 g/m² 100% nylon taffeta
- Inner Insulation:
- 10 g/ft PrimaLoft® ThermoBall™ synthetic insulation; 60 g PrimaLoft® Silver Insulation Eco
- Lifetime Warranty
- Care Instructions:
- Machine Wash
Keep your marathon training on track in any weather with this extremely lightweight highly wind-resistant jacket that features breathable FlashDry™ fabric to manage moisture and a durable water-repellent (DWR) finish to shed light rain. Reflective details ensure your visibility in ...
Get the versatile coverage you need this ski season with a customizable three-in-one jacket system that prepares you for unpredictable conditions. The exterior jacket is constructed of breathable TNF™ Apex ClimateBlock for water-resistant windproof protection when you're skiing on piste. ...
The ultimate summit jacket for extreme cold offers responsibly sourced 800 fill down and a welded Z-baffle construction to deliver maximum warmth and protection. The hyper-durable FuseForm™ construction provides targeted tear resistance while articulated patterning offers top alpine mobility. Part ...
Get reliable protection from the elements even deep in the wilderness with this unlined water- and windproof DryVent™ jacket that features four-way stretch strategically placed vents and an adjustable hem and wrist cuffs to avoid snags on the trail.


Saturday, 20 February 2010
I Just Can't Shake a Feeling
New tune using a bit of retro synthpop. The instrumental of Depeche Mode's 'Just Can't Get Enough' mashed with vocals from Black Eyed Peas 'I Got a Feeling' and The Outhere Brothers 'Shake It'.
Click the pic to download or play using Fairtilizer player.


text:
Every human story is filled with something worth seeing or hearing. Some of those experiences are painless while others are quite painful . But each is in some way priceless. I grow when I can dare to see life through someone else’s eyes. Their experience may be different than mine but worth listening to and understanding.
Writing fiction has been eye opening for me. It allows me to tell the human story in a way that I can envision it while making me more aware of my human connection and the world in which I live. A kind of therapy, if you will, it helps me come to terms with what I see happening around me. Through fictional characters I can deal with tough subjects in a way that is hopefully entertaining, and the story becomes a part of me in the process. When I finish writing a book I’m left with an empty feeling until my characters run off to another adventure and I once again paint a story using words.
Life has not been easy for the whole world lately. Besides the normal trials of life, we have been impacted by a pandemic, hurricanes, and wildfires along with wars, and enough division to last a lifetime.
I have found myself having to take a step back more often to gather my thoughts. I want to see people as more than mere numbers, and to feel empathy for what I see happening around me. When I read this poem it touched me and I hope it touches you too.
I’ll paint you a rainbow to hang on the wall, to brighten your heart when the gray shadows fall, on the canvas of joy outlasting the years, with a soft brush of sweetness to dry all your tears.
I’ll paint you a rainbow with colors of smiles that glow with sincerity over the miles. On a palette of words, I will tenderly blend tones in treasures of sunlight and wind.
I’ll paint the rainbow that reaches so wide, your sighs and your sorrows will vanish inside. And deep in the center of each different hue, a memory fashioned especially for you.
So lift up your eyes, for suspended above, a rainbow designed by the fingers of love…
Poem, ‘I’ll Paint You A Rainbow’ by Grace E.
EasleyPhoto Credit: Rosie Hardy Photography


text:
After a full slate of games on Wednesday, there are only six teams taking the court in the Association tonight. While that limits the amount of player props to consider, we still found three of our favorites tonight with odds courtesy of DraftKings Sportsbook.
Franz Wagner over 18.5 points vs. Jazz (-110)
While Wagner hasn’t cleared this over in either of his last two games, he’ll be going against a Jazz team that has the fourth-worst defensive rating in the NBA and is on the second night of a back-to-back set. The Jazz have had multiple players score 20 points on them in four of their five games this year (it would have been five but Jaren Jackson had 19 last night), and have struggled to defend against the small forward position. Wagner had 19 against the Rockets in his season opener (who have the same defensive ranking as Utah), and he’s taken at least 12 shots in every game, so I’m expecting him to fill it up tonight.
Cade Cunningham under 22.5 points vs. Pelicans (-110)
I’m riding an interesting Cunningham trend. After dropping 30 points on opening night, he only went for 12 the next night. That trend continued over the weekend, as he dropped 25 on the Bulls on Saturday before only scoring 17 against the Thunder on Monday. He dropped 30 against the Trail Blazers last night, so i’m expecting another lower-scoring outing tonight against a Pelicans team that has the fifth-best defensive rating in the NBA.
Victor Wembanyama over 6.5 rebounds vs. Suns (-120)
Wembanyama had eight rebounds against the Suns on Tuesday, and I’m expecting him to clear that line once again. The Suns rank 15th in defensive rebound percentage (71.3%) and have allowed a double-digit rebounder in three of the four games they’ve played in this season. I like Wemby’s chances to grab at least seven boards.


text:
ARCADIA, Three World Premieres & More Set for Yale Rep's 2014-15 Season
YALE REPERTORY THEATRE, dedicated to the production of new plays and bold interpretations of classics that make immediate connections to contemporary audiences, announces its 2014-15 season, which will include two modern masterpieces and three world premieres commissioned by Yale Rep, in addition to three titles to be announced as part of the NO BOUNDARIES series of global performances.
The season will feature new productions of ARCADIA by multiple Tony and Oscar winner Tom Stoppard, one of the most accomplished writers of the 20th and 21st centuries, staged by Artistic Director James Bundy; and THE CAUCASIAN CHALK CIRCLE by poet-playwright Bertolt Brecht, whose mordantly funny and strikingly theatrical body of work continues to inspire generations of theatre artists, directed by OBIE Award winning Resident Director Liz Diamond.
Three new plays commissioned by Yale Repertory Theatre will have their world premieres as part of the 2014-15 season: WAR by Branden Jacobs-Jenkins (whose acclaimed play Appropriate is currently running in New York), directed by Lileana Blain-Cruz; FAMILIAR, an intergenerational comedy-drama by OBIE Award winner and Pulitzer Prize nominee Danai Gurira, directed by Rebecca Taichman; and ELEVADA, a romantic comedy by Sheila Callaghan, profiled by Marie Claire as one of "18 Women Who Are Changing the World," directed by Jackson Gay.
Yale Rep's 2014-15 season will also feature three titles, to be announced, presented at part of the NO BOUNDARIES series of global performances.
"Yale Rep's 2014-15 season offers an astonishing lineup of gifted theatre artists and a breathtaking diversity of emotions, experiences, and perspectives that ultimately reflect and celebrate our shared humanity," says Artistic Director James Bundy. "I'm delighted that Yale Rep continues to be a home for work by artists such as Tom Stoppard, Danai Gurira, Rebecca Taichman, Liz Diamond, and Jackson Gay, who are well known already to many Yale Rep audience members, and I'm thrilled to introduce Branden Jacobs-Jenkins, Lilieana Blain-Cruz, and Sheila Callaghan, all of whom are making their Yale Rep debuts."
ABOUT THE 2014-15 SEASON
Amidst illicit passions and professional rivalries on an English country estate, a brilliant young pupil proposes a startling scientific theory well beyond her own comprehension. More than 200 years later, two academic adversaries try to piece together puzzling clues from the past in their quest for an increasingly elusive truth. Arcadia is an achingly romantic and heartbreakingly funny waltz of the mind and the body, dancing across centuries.
Arcadia by Tom Stoppard, the four-time Tony and Academy Award winning author of The Coast of Utopia, Rough Crossing (Yale Rep, 2008), and Shakespeare in Love, has been hailed as "the perfect blend of brains and emotion, wit and heartache" (The New York Times). Artistic Director James Bundy, whose Yale Rep credits include Hamlet, A Delicate Balance, and A Woman of No Importance, stages this new production of "the greatest play of our age" (The Independent).
Tensions escalate between Tate and Joanne at the hospital bedside of their comatose mother. As they attack each other's smallest words and biggest choices, they are ambushed by two strangers who make a shocking claim about their grandfather's WWII tour of duty. War is a wildly provocative, bracingly funny, and all-too-human portrait of a family navigating the landmines of the past as they try to broker peace with each other-and themselves-in the present.
Acclaimed playwright Branden Jacobs-Jenkins makes his Yale Rep debut with the world premiere of War. His plays include Neighbors, An Octoroon, and Appropriate, which the New York Times praised as "highly entertaining, sharp, and peopled with complex characters." Lileana Blain-Cruz recently directed Christina Anderson's Hollow Roots for The Public Theater's Under the Radar Festival.
In a snowy Midwestern suburb, Marvelous and Donald are preparing for the marriage of their eldest daughter. Clashes erupt within the family when the first-generation American bride-to-be insists on observing a traditional Zimbabwean wedding ritual. Familiar is a richly funny and deeply moving new play about the complicated relationships between mothers and daughters, sisters, wives and husbands-the customs they keep, and the secrets they keep buried.
Born in the U.S. and raised in Zimbabwe, OBIE and Whiting Writers Award winning playwright Danai Gurira is the author of the critically acclaimed plays Eclipsed (Yale Rep, 2009), the Pulitzer Prize finalist In the Continuum (co-written with Nikkole Salter; Yale Rep, 2007), and The Convert. Rebecca Taichman's previous Yale Rep credits include the world premieres of David Adjmi's Marie Antoinette and The Evildoers.
In the entirely fabricated country of Grusinia, a sturdy young servant named Grusha rescues the abandoned baby boy of the Governor overthrown in a violent palace coup. As civil order is restored, the exiled ruler's wife returns to reclaim her child-and the two mothers bring their case before a cynical, battle-weary judge. An epic fable set against the harrowing backdrop of total war, The Caucasian Chalk Circle traces the thin lines between law and justice, blood and family.
OBIE Award winning Resident Director Liz Diamond (The Winter's Tale, Happy Now?, Saint Joan of the Stockyards) returns to Yale Rep with a new production of the great 20th-century poet-playwright Bertolt Brecht's playfully theatrical, lyrical, and bitingly satiric masterpiece, The Caucasian Chalk Circle.
Please note: The Caucasian Chalk Circle is Yale Rep's 2014-15 WILL POWER! production. The run includes two 10:15AM performances on April 8 and 9, 2015, available only to high school groups. For information on WILL POWER! performances, please contact Ruth M. Feldman at (203) 432-8425 or email@example.com.
New York City. Right now. Ramona's going on lots of first dates but is intentionally sabotaging her chances for a second. Khalil, a social media superstar, is about to close a huge deal that will take him completely off the market. They'll do anything to float above their own lives, even as fate tries to pull them both back down to earth. Elevada is a warm, witty, and wise romantic comedy about the fear of being alone-and the fear of not being alone.
A finalist for the 2014 Susan Smith Blackburn Prize, Elevada marks the Yale Rep debut of Sheila Callaghan, "a gutsy writer with a gift for creating vivid images rooted in the emotional life of her characters" (The New York Times), whose plays include That Pretty Pretty; or, The Rape Play and Roadkill Confidential. The world premiere of Elevada is directed by Jackson Gay, who has staged These Paper Bullets! and The Intelligent Design of Jenny Chow at Yale Rep.
Three additional titles, presented as part of the NO BOUNDARIES series of global performances, will be announced in the fall.

domain: literature


Kyosho Carbon Composite Gear Box (RB6)
$1.99 Flat Rate Shipping!
This item is eligible for $1.99 flat rate shipping Learn More
This item earns up to 14 VIP Reward Points Learn More
This is the Kyosho Carbon Composite Gear Box. This gear box is a replacement for the Kyosho Ultima RB6, but can also be used as an optional upgrade for the Ultima RB5, RT5, SC and DB vehicles.
This product was added to our catalog on March 1, 2013.


Designed to reflect the passion of the fans and nation the shirt incorporates a striking interpretation of the Sol de Mayo inspired by Carlos Paez Vilaro’s illustration of the iconic symbol. This powerful image brings great pride to the nation and is the perfect motivation for players and fans alike to come together and rise above the opposition on the world’s biggest stage.
The unforgettable ‘Celeste’ blue rekindles memories of the first time you pulled on the jersey and watched your home heroes on TV, that first time you felt real joy, your First Passion.
Subtler details are incorporated into the shirt to further enforce the feeling of passion and pride. These include a subtle nod to the National Pavilion underneath the back of the collar, whilst inside the sleeve is the inscription ‘La Celeste’ and the hashtag #ELEQUIPOQUENOSUNE can be found on the underside of the bottom of the shirt.
In addition, the shirt is engineered using PUMA’s highly developed evoKNIT Thermoregulation technology an enhanced moisture management and adaptive cooling system that helps create the perfect on-pitch body temperature. Combined with its seamless construction, the jersey offers the ultimate in fit and mobility for optimum performance.
Getting into the details you will notice engineered cooling fins in key zones on the shirt that act as channels regulating your bodies temperature, whilst dryCELL technology provides moisture management across the fabric with high-performance yarns that wick sweat away from the body. All this is packaged into the seamless evoKNIT construction of the shirt that provides a lightweight ‘second skin’ feel so the fabric moves with the players body and not against it, allowing for better freedom of movement on the pitch.
An especially gifted procrastinator who occasionally uses words the in order correct.


text:
Fire training clipart free free firefighter cliparts download free clip art free fire training free clipart
In case, you still need to add any other note or detail, do it on the back of your invitation card. Build the excitement by delivering your invitations with a small bottle of bubbles or a pop-up sponge! You can also get personalized Sponge-Bob party invitations from Birthday in a Box. Hang blue streamers (two shades would be great) all around the party area and also keep some SpongeBob printable coloring pages so the younger children can color and enjoy. In my household using Hello Kitty coloring pages is one of my kids most cherished activities. I think this is great as it is so low cost and easy to keep them entertained. Few activities for kids are as stress-free, that's for certain. In the modern busy world, this hobby is a calm oasis. Personally I enjoy the quiet moments that coloring brings. It is a welcome break from all the music and shouting that is so common with youngsters. Hello Kitty coloring pages especially continue to be one of the most popular and demanded by little ones of all ages, especially with young girls! Children who lack intellectual maturity and emotional satisfaction can be appreciated through the assignment of task involving coloring their favorite characters. In the beginning they may start up with whacky coloring but eventually their skills tend to enhance. They gain emotional satisfaction and also build up essential motor skills like concentration of eyes and hand, hand dexterity etc. it is a kind of wholesome enjoyable task. Most kids love Disney characters very much and when they get their hands onto coloring they forget everything else. They become like angels and always try to express their best by coloring. Characterize with extraordinary size of head and asymmetrical body, she is Dora the Explorer. You will also be enticed with her short straight hair and thick bangs. Dora is a portrayal of a cute modest child.
We found 24++ Images in Fire training clipart free:
#fire training academy | fire training center | fire training facilities | fire training ideas | fire training props | fire training resources | fire training structures | fire training towers | fire training videos |


text:
Background:Dilated cardiomyopathy (DCM) is among the most prevalent causes of heart failure and sudden cardiac death worldwide. Nuclear lamin protein coding genes are believed to have a definitive role in the DCM phenotype. The rs505058 thymine (T) to cytosine (C) polymorphism in the lamin-A gene is one of the most common mutations reported in familial DCM in previous studies.
Objectives:We aimed to demonstrate the possible role of this SNP in the lamin A and C (LMNA) gene in the incidence of DCM among the south of Iran population.
Methods:This case-control study included 65 patients with familial DCM and 70 healthy participants in south Iran. DNA was extracted from nucleated blood cells, and polymerase chain reaction (PCR) was performed. First, the sequence of the LMNA gene was investigated. Then the frequency of alleles C (mutated allele) and T (normal) of the LMNA gene (rs505058) was compared between the case and controls.
Results:No notable differences were seen in gender and age between the case and controls. The genotype frequencies of the LMNA rs505058 T/C polymorphism were 6% (CC), 31% (CT), and 63% (TT) amongst cases, and 1% (CC), 13% (CT) and 86% (TT) in controls. For allele level comparison, the LMNA rs505058 C allele has been shown to have a statistically significant association with an enhancement in the risk of dilated cardiomyopathy (OR = 2.92; 95% CI = 1.05 to 8.15, P-value = 0.034)
Conclusions:The results indicated that the rs505058 T/C SNP of the LMNA gene is associated with familial DCM patients in the Iranian population.
Dilated cardiomyopathy (DCM) is among the most prevalent causes of heart failure and sudden cardiac death worldwide (1). The prevalence of DCM is four in 10,000 people, 30 - 50% of whom are afflicted with familial dilated cardiomyopathy (2). Despite immense medical advances in the last decades, such as the feasibility of heart transplantation and the availability of proper pharmaceutical agents, the prognosis of this disease has not enhanced significantly; therefore, early diagnosis and prevention of the progression remain the best option (3). Previously, environmental factors were attributed to the pathogenesis of this disease; in addition, recently, the genetic predisposition of the disease with the Mendelian mode of inheritance (mostly autosomal dominant) has been proven (4). Familial DCM is clinically and diagnostically the same as other forms of DCM; consequently, careful attention to family history is essential in the approach to DCM patients. The diagnosis of familial DCM is based on having one relative diagnosed with DCM or a history of sudden unexplained death in one of the first-degree relatives aged under 35 (5, 6). More than 50 genes have been discovered to be involved in the pathogenesis of familial DCM (7). Mutations in the nucleophilic A-type lamins, including the lamin A and C (LMNA) gene located on the long arm of chromosome 1, account for 5% to 8% of familial forms of DCM (8). According to the latest reports by American Heart Association (AHA), this gene is believed to have a definitive role in the DCM phenotype (9). LMNA protein coding gene has 12 exons in which a defect can cause DCM with or without conductive system abnormalities (10). It is the coding section for lamin-A and lamin-C proteins in the protein network of the inner nuclear membranes of the non-proliferative cells. Coding unfunctional lamin-A and lamin-C might impair nuclear membrane function, which would cause myocytes’ disintegration and destruction of the cardiac tissue (11). More than forty different mutations in the LMNA gene are associated with various conditions such as autosomal dominant Emery-Dreifuss muscular dystrophy, limb-girdle muscular dystrophy, familial DCM, and familial partial lipodystrophy (12, 13). Rs505058 polymorphism is an alteration in the Lamin gene’s third organic base of the 446th codon located on exon seven, by which a cytosine base substitutes thymine. This single nucleotide polymorphism (SNP) is one of the most common mutations seen in familial DCM (14, 15).
Is this SNP seen in patients with DCM in the Iranian population or not? Knowing these correlations might be helpful in future gene-based treatments and early diagnosis of this disease.
We designed this study to evaluate the association of rs505058 T/C SNP in the LMNA protein-coding gene with familial dilated cardiomyopathy in Iranian patients.
3.1. Study Design
The study followed a case-control protocol. Seventy DCM patients with at least one relative diagnosed with DCM or any unexplained sudden cardiac deaths in their first-degree relatives younger than 35 were enrolled in the study as the case group. Seventy healthy participants with normal left ventricular ejection fraction (LVEF) matched to the case group regarding their sex and age entered the study as the control group.
Written informed consent was obtained from all participants. The study was conducted in accordance with the Declaration of Helsinki and was approved by the Ethics Committee of Jahrom University of Medical Sciences, Iran. All the individuals in the case and control groups underwent detailed history taking, physical examination, 12-lead electrocardiography, and transthoracic echocardiography. The considered echocardiographic criteria for diagnosis of dilated cardiomyopathy were as follows (14):
(1) Fractional shortening < 25% (reduction of > 2 standard deviations) and/or l LVEF < 45% (reduction of > 2 standard deviations);
(2) Left ventricular end-diastolic diameter more than 117% of the predicted value for age and total body surface area.
The study’s inclusion criteria were: Diagnosis of DCM in the patient and at least one of their first-degree family members by the mentioned criteria.
The study’s exclusion criteria were: Lack of proof for DCM, incompliance with the patients, unwillingness to enter the study, and the lack of family history for DCM.
3.2. Genetic Study
A 5 to 20 cc of the participants’ median cubital vein blood was collected in tubes containing EDTA to avoid clot formation. Then deoxyribonucleic acid (DNA) was extracted from the nucleated blood cells according to the instructions of the kit company (Sinagen Co., Iran). DNA was quantified spectrophotometrically prior to being used in the polymerase chain reaction (PCR). Next, extracted DNA was stored at -20°C before the PCR. The lamin gene sequence containing the rs505058 polymorphism was investigated using PCR amplification with specific forward GAGATCCACGCCTACCGCAAG and Reverse primer: AGCCAAAGAGTCCAGGAGCCA (Table 1).
Specific Primers That Were Used
PCR reactions were performed in master mix tubes (Amplicon, Denmark). The reaction volume of 20 μL contained template DNA 2.5 μL, primers each 10 pmol, magnesium chloride 1.5 μM, dNTP Taq polymerase enzyme 250 μM each unit, and sterile water to 20 μL total reaction volume. The PCR reaction was done using a thermocycler (thermal cycler). The PCR program was as follows: 94°C for 5 min, with 35 cycles of 94°C for 40 seconds, 61°C for 30 seconds, and a final extension at 72°C for one minute and 72°C for 10 minutes, and ended. Then, tube samples were stored at -20°C. Gene runner software was used to determine the duplicated parts of primers, and gene sequences were confirmed by the blast program. The primers yielded a PCR product of 533 base pairs (bp) which were wholly digested with the restriction endonucleases Ear I for 24 h at 37°C to detect the rs505058 (T/C) polymorphism. Amplification products with the T allele (wild type) were digested into 219 and 314 bp DNA fragments with the restrictive enzyme Ear I (TT genotype and CT genotype). But the homozygote genotype CC did not undergo digestion with the enzyme. DNA fragments obtained after restrictive enzyme digestion and the DNA size marker were electrophoresed on a 3% gel agarose and stained with ethidium bromide.
3.3. Statistical Analysis
The data were registered into IBM SPSS for Windows, version 21.0 (SPSS Inc. Released 2007. Chicago, IL, USA) for statistical analysis. The quantitative variables were reported as mean, and standard deviation, and the qualitative variables were reported as frequency and percentages. A chi-square test was used to evaluate the association of rs505058 SNP with familial DCM. P-values less than 0.05 were counted as statistically significant.
A total of seventy cases and seventy controls entered the study. Unfortunately, five samples of the case group were missed during the PCR and electrophoresis procedures which were withdrawn from statistical analysis.
The mean age of the participants was 28.87 ± 15.30 years for the case group and 29.18 ± 15.409 years for the control group. The age difference between the two groups was not statistically significant (P-value = 0.906).
In the control group, 57.4% (n = 40) of the participants were male, and 42.6% (n = 30) of them were female, while in the case group, 53.2% (n = 35) of the participants were male and 46.8% (n = 30) of the participants were female. The sex difference between the two groups was not statistically significant (chi-square test P-value = 0.700) (Table 2).
|Variables||Control Group (n = 70)||Case Group (n = 65)||P Value|
|Male||40 (57.1)||35 (53.8)|
|Female||30 (42.9)||30 (46.2)|
|Age (y)||29.18 ± 15.40||28.87 ± 15.3||0.906|
The Hardy-Weinberg equilibrium was used to survey the repartition of the rs505058 LMNA gene SNP genotypes, demonstrating a success rate of 100%. The wild-type TT genotype (P-value = 0.011) were significantly higher in the control group, while the mutated heterozygote genotype CT (P-value = 0.002) were significantly higher among the cases. Likewise, the mutated homozygote genotype CC was higher in the cases, but the relation was not statistically significant (Table 3). Considering the allele level comparison, the LMNA rs505058 C allele was accompanied by an enhancement in the incidence of dilated cardiomyopathy (OR = 2.92; 95% CI = 1.05 to 8.15, P-value = 0.034).
Genotype and Allele Distributions of the rs505058 SNP in the LMNA Gene in Case and Controls
The correlation between SNPs of the LMNA gene and DCM had yet to be previously studied in Iran; However, this study is the first study on the rs505058 polymorphism of the LMNA gene in Iranian population (16, 17). We found that the prevalence of rs505058 T/C SNP of the LMNA gene was higher in DCM patients than in healthy individuals in the Iranian population. C allele has shown a significant direct relationship with the prevalence of familial DCM in our studied population. In addition, the mutated heterozygote CT and homozygote CC genotypes prevalence were higher among the DCM patients compared to our controls. However, this relation was not statistically significant for the homozygote CC genotype, possibly due to the small sample size. Our findings were in line with previous works conducted on this issue worldwide. The higher rates of this mutation in patients indicate a direct relationship between this genetic alteration and the pathogenesis of DCM; however, the exact mechanism of this relationship is yet to be found.
Dilated cardiomyopathy is a disease of cardiac myocytes characterized by systolic and diastolic dysfunction. DCM can be presented as heart failure, thromboembolism, and sudden cardiac death (18, 19). Currently, mutations in more than 50 genes have been found to be associated with autosomal dominant DCM. LMNA gene encodes nuclear envelope proteins lamin A and lamin C, located on chromosome 1, and is one of the strong and definitive associating genes (20). Patients with symptomatic DCM with LMNA mutations seem to have a poor prognosis and carry a greater risk of complications such as dysrhythmias, sudden cardiac death, and heart failure than mutations in other loci (21).
Fatkin et al. were the first to describe the association between LMNA mutations with FDC accompanied by conductive system abnormalities such as sinus bradycardia, atrioventricular blocks, or atrial arrhythmias (10). Conductive system abnormalities might prompt the need for pacemakers and can become life-threatening in longstanding unattained LMNA-related DCM (22). Arbustini et al. introduced K97E, E111X, R190W, and E317K mutations of LMNA to be associated with FDC (13). They reported that around one-third of patients with DCM atrioventricular blocks carry mutations in their LMNA gene (13). Patients with symptomatic DCM with LMNA mutations have a poorer prognosis and take a greater risk of systolic and diastolic dysfunction, thromboembolic events, and sudden cardiac death than mutations in other loci (18). In patients with LMNA mutations, cardiac arrhythmias such as atrial fibrillation and ventricular tachycardia might be the first presenting symptoms long before the progression of DCM. It is yet to be evaluated if antiarrhythmic pharmacotherapy has a role in decreasing the pace or halting the process of the pathogenesis of DCM (12).
Gupta et al. reported that large deletions in the LMNA gene could cause insufficiency of lamin A/C proteins, thus disrupting the typical architecture of the nuclear envelope; however, they could not find any relations between SNPs of the LMNA gene and the severity of DCM (8).
Recently many studies have focused on the association between different SNPs of the LMNA gene and the pathogenesis of DCM (22-25). In rs505058 SNP, an alteration from thymine to cytosine in the 3rd base of codon 446 results in a nonsense variation to Aspartic acid at exon 7 of the LMNA gene (26, 27). Banerjee et al. reported the linkage between rs505058 SNP of the region encoding the central rod domain of lamin A/C proteins in an Indian population in 2015 (14). Sylvius et al. evaluated the relationship between two distinct LMNA mutations with cellular alterations (20). They found that the D192G mutation, in contrast to the R541S mutation, was associated with significant changes in the ultra-structure of the nuclear envelope. Furthermore, patients with D192G haploinsufficiency had a dimmer prognosis than patients with heterozygous R541S mutation. They also speculated that such mutations interfere with sumoylation-dependent cellular activities such as RNA transcription (20). Such underlying mechanisms might be involved in the pathogenesis of familial DCM associated with rs505058 SNP. However, these theories need further evaluation with more extensive studies.
Another proposed underlying mechanism for the pathogenesis of LMNA-related DCM comprises defective chromatin organization in the proliferating cells and signal transduction of non-proliferating cells (10, 20). Furthermore, LMNA mutations were found in more than 85% of patients with concurrent DCM and muscular dystrophy and in one-quarter of patients with isolated conductive system abnormalities, which prompts further evaluation of the genetic causes of conduction system disease (28).
This study should be done in multi centers from different parts of the country, enrolling more participants. Moreover, in this study, long-term follow-up of the patients was not achieved, which can be counted as a limitation.
We conclude that the rs505058 T/C SNP in the LMNA gene is associated with the higher incidence of familial DCM among the south of Iran population; however, due to the limited number of participants in this study, further research is encouraged.
Lakdawala NK, Winterfield JR, Funke BH. Dilated cardiomyopathy. Circ Arrhythm Electrophysiol. 2013;6(1):228-37. [PubMed ID: 23022708]. [PubMed Central ID: PMC3603701]. https://doi.org/10.1161/CIRCEP.111.962050.
Baig MK, Goldman JH, Caforio AL, Coonar AS, Keeling PJ, McKenna WJ. Familial dilated cardiomyopathy: cardiac abnormalities are common in asymptomatic relatives and may represent early disease. J Am Coll Cardiol. 1998;31(1):195-201. [PubMed ID: 9426040]. https://doi.org/10.1016/s0735-1097(97)00433-6.
Taylor MR, Fain PR, Sinagra G, Robinson ML, Robertson AD, Carniel E, et al. Natural history of dilated cardiomyopathy due to lamin A/C gene mutations. J Am Coll Cardiol. 2003;41(5):771-80. [PubMed ID: 12628721]. https://doi.org/10.1016/s0735-1097(02)02954-6.
McNally EM, Mestroni L. Dilated Cardiomyopathy: Genetic Determinants and Mechanisms. Circ Res. 2017;121(7):731-48. [PubMed ID: 28912180]. [PubMed Central ID: PMC5626020]. https://doi.org/10.1161/CIRCRESAHA.116.309396.
Villard E, Perret C, Gary F, Proust C, Dilanian G, Hengstenberg C, et al. A genome-wide association study identifies two loci associated with heart failure due to dilated cardiomyopathy. Eur Heart J. 2011;32(9):1065-76. [PubMed ID: 21459883]. [PubMed Central ID: PMC3086901]. https://doi.org/10.1093/eurheartj/ehr105.
Ku L, Feiger J, Taylor M, Mestroni L, Familial Cardiomyopathy R. Cardiology patient page. Familial dilated cardiomyopathy. Circulation. 2003;108(17):e118-21. [PubMed ID: 14581388]. https://doi.org/10.1161/01.CIR.0000097493.70422.50.
Gupta P, Bilinska ZT, Sylvius N, Boudreau E, Veinot JP, Labib S, et al. Genetic and ultrastructural studies in dilated cardiomyopathy patients: a large deletion in the lamin A/C gene is associated with cardiomyocyte nuclear envelope disruption. Basic Res Cardiol. 2010;105(3):365-77. [PubMed ID: 20127487]. [PubMed Central ID: PMC3934843]. https://doi.org/10.1007/s00395-010-0085-4.
Jordan E, Peterson L, Ai T, Asatryan B, Bronicki L, Brown E, et al. Evidence-Based Assessment of Genes in Dilated Cardiomyopathy. Circulation. 2021;144(1):7-19. [PubMed ID: 33947203]. [PubMed Central ID: PMC8247549]. https://doi.org/10.1161/CIRCULATIONAHA.120.053033.
Fatkin D, MacRae C, Sasaki T, Wolff MR, Porcu M, Frenneaux M, et al. Missense mutations in the rod domain of the lamin A/C gene as causes of dilated cardiomyopathy and conduction-system disease. N Engl J Med. 1999;341(23):1715-24. [PubMed ID: 10580070]. https://doi.org/10.1056/NEJM199912023412302.
Crasto S, My I, Di Pasquale E. The Broad Spectrum of LMNA Cardiac Diseases: From Molecular Mechanisms to Clinical Phenotype. Front Physiol. 2020;11:761. [PubMed ID: 32719615]. [PubMed Central ID: PMC7349320]. https://doi.org/10.3389/fphys.2020.00761.
Arbustini E, Pilotto A, Repetto A, Grasso M, Negri A, Diegoli M, et al. Autosomal dominant dilated cardiomyopathy with atrioventricular block: a lamin A/C defect-related disease. J Am Coll Cardiol. 2002;39(6):981-90. [PubMed ID: 11897440]. https://doi.org/10.1016/s0735-1097(02)01724-2.
Banerjee A, Ghoshal PK, Sengupta K. Novel linkage of LMNA Single Nucleotide Polymorphism with Dilated Cardiomyopathy in an Indian case study. Int J Cardiol Heart Vasc. 2015;7:99-105. [PubMed ID: 28785654]. [PubMed Central ID: PMC5497236]. https://doi.org/10.1016/j.ijcha.2015.02.008.
Rosenbaum AN, Agre KE, Pereira NL. Genetics of dilated cardiomyopathy: practical implications for heart failure management. Nat Rev Cardiol. 2020;17(5):286-97. [PubMed ID: 31605094]. https://doi.org/10.1038/s41569-019-0284-0.
Yousefi HA, Asghari Moghaddam N, Jafari Fesharaki M. Association between the rs538089 of the LMNA gene and dilated cardiomyopathy in Iranian patients. Iran Heart J. 2020;21(4):92-9.
Sayadi A, Parto S, Moghaddam NA. Evaluation of the association between dilated cardiomyopathy and single nucleotide polymorphisms of the lamin A/C gene in the Iranian population. Gene Rep. 2022;26. https://doi.org/10.1016/j.genrep.2021.101461.
Tayal U, Ware JS, Lakdawala NK, Heymans S, Prasad SK. Understanding the genetics of adult-onset dilated cardiomyopathy: what a clinician needs to know. Eur Heart J. 2021;42(24):2384-96. [PubMed ID: 34153989]. [PubMed Central ID: PMC8216730]. https://doi.org/10.1093/eurheartj/ehab286.
Sylvius N, Bilinska ZT, Veinot JP, Fidzianska A, Bolongo PM, Poon S, et al. In vivo and in vitro examination of the functional significances of novel lamin gene mutations in heart failure patients. J Med Genet. 2005;42(8):639-47. [PubMed ID: 16061563]. [PubMed Central ID: PMC1736117]. https://doi.org/10.1136/jmg.2004.023283.
Tayal U, Prasad S, Cook SA. Genetics and genomics of dilated cardiomyopathy and systolic heart failure. Genome Med. 2017;9(1):20. [PubMed ID: 28228157]. [PubMed Central ID: PMC5322656]. https://doi.org/10.1186/s13073-017-0410-8.
Hershberger RE, Jordan E. Dilated Cardiomyopathy Overview. In: Adam MP, Mirzaa GM, Pagon RA, Wallace SE, Bean LJH, Gripp KW, et al., editors. GeneReviews((R)). Seattle, USA: University of Washington; 1993.
Hermida-Prieto M, Monserrat L, Castro-Beiras A, Laredo R, Soler R, Peteiro J, et al. Familial dilated cardiomyopathy and isolated left ventricular noncompaction associated with lamin A/C gene mutations. Am J Cardiol. 2004;94(1):50-4. [PubMed ID: 15219508]. https://doi.org/10.1016/j.amjcard.2004.03.029.
Li X, Luo R, Mo X, Jiang R, Kong H, Hua W, et al. Polymorphism of ZBTB17 gene is associated with idiopathic dilated cardiomyopathy: a case control study in a Han Chinese population. Eur J Med Res. 2013;18(1):10. [PubMed ID: 23570452]. [PubMed Central ID: PMC3626695]. https://doi.org/10.1186/2047-783X-18-10.
Krohne G, Benavente R. The nuclear lamins. A multigene family of proteins in evolution and differentiation. Exp Cell Res. 1986;162(1):1-10. [PubMed ID: 2415378]. https://doi.org/10.1016/0014-4827(86)90421-0.
Teekakirikul P, Kelly MA, Rehm HL, Lakdawala NK, Funke BH. Inherited cardiomyopathies: molecular genetics and clinical genetic testing in the postgenomic era. J Mol Diagn. 2013;15(2):158-70. [PubMed ID: 23274168]. https://doi.org/10.1016/j.jmoldx.2012.09.002.
van Tintelen JP, Hofstra RM, Katerberg H, Rossenbacker T, Wiesfeld AC, du Marchie Sarvaas GJ, et al. High yield of LMNA mutations in patients with dilated cardiomyopathy and/or conduction disease referred to cardiogenetics outpatient clinics. Am Heart J. 2007;154(6):1130-9. [PubMed ID: 18035086]. https://doi.org/10.1016/j.ahj.2007.07.038.

domain: medical


text:
Census Outreach Captain
SALARY: $25.00 P/H
The YMCA of Greater New York is here for all New Yorkers — to empower youth, improve health, and strengthen community. Founded in 1852, today the Y serves a diverse population of more than half a million New Yorkers who learn, grow, and thrive through programs and services at our 24 branches. Community is the cornerstone of the Y. Together, we connect active, engaged New Yorkers to build stronger communities.
The Jamaica YMCA is seeking a Census Outreach Captain who will work with the Public Affairs team, a YMCA branch, and external partners to build awareness and mobilize census response efforts. The Census Outreach Captain will be responsible for coordinating neighborhood-based efforts related to Census 2020.
- Identify and cultivate relationships with community and government partners.
- Engage in extensive community outreach, both through formal partners such as houses of worship and informal spaces such as barber shops.
- Coordinate the implementation of census plan at the Jamaica YMCA branch, including distributing educational materials, helping organize community forums, and organizing volunteers for pop-up census centers.
- Track and compile data from neighborhood efforts.
- Other duties as assigned.
- Adhere to all Department of Health, YMCA of Greater New York, and funder standards, expectations, and regulations.
- Actively participate in all training sessions and designated meetings.
- High School diploma or equivalent.
- Minimum of six (6) months of experience developing and maintaining external partnerships, and community organizing.
- Familiarity with New York City a must.
- Knowledge of Microsoft Word and Excel.
- Knowledge of Publisher or other design program.
- Knowledge of census process desired.
- Bilingual a plus.
- Excellent written and oral communication skills.
We offer an exciting and innovative work environment with an organizational culture committed to serving all members of our community. As a leading not-for-profit, community service organization, our Association relies heavily on fundraising to support the wide range of programs we proudly provide the communities we serve. Our expectation is that all staff promotes participation of their branch fundraising efforts in some capacity.
If you would like to be a member of our dynamic team, please send your cover letter and resume with subject line “Census Outreach Captain – Jamaica” to firstname.lastname@example.org or to:YMCA of Greater New York
Attn.: Human Resources
5 West 63rd Street – 6th Floor
New York, NY 10023
EQUAL OPPORTUNITY EMPLOYER ♦ DRUGFREE WORKPLACE
Auxiliary aids and services are available upon request to individuals with disabilities

domain: sociology


text:
Why is steam good for natural and low porosity hair?
At DHA, we're big fans of moist heat, aka steam. because of the way it can bring hydration to the most resistant, impenetrable low porosity hair.
The 'steam' your hair actually comes into contact with when using a steamer is really water vapour, droplets of H2O that have gotten hot and light enough to float in the air, but not quite hot enough to be steam.
These droplets work so well at hydrating low porosity hair because when they're in this vaporous state, water molecules have way more energy than in their regular liquid form. They move faster and more freely, which makes it quicker and easier for these droplets (aka moisture) to penetrate your hair strand.
Another big reason why hair steamers hydrate better than normal, liquid water is because the surface tension is reduced to almost zero. You know how when water is still, whether in a glass or a calm lake, the surface looks really flat, almost like it has a skin over it? This is because of surface tension, or the way the molecules at the surface of a liquid naturally cling together.
Water's surface tension makes it harder for it to penetrate your hair since its own surface is holding it back. Add that to the resistance of low porosity hair to absorb pretty much anything and you'll see why it takes so long for your hair to get wet - if it gets wet at all.
For the water droplets released by the steamer, surface tension is effectively gone. This is what frees them to float onto and drift into your hair, making their way into the few gaps your low porosity hair permits - and finally getting it hydrated.
All this added moisture is what makes deep conditioning low porosity hair with steam so effective.
What is better a hair steamer, a heat cap or a dryer?
Using just heat, e.g., a heat cap, does make your conditioner more fluid and so more able to give good coverage. But since heat caps don't emit water vapour, they don't deliver the level of hydration you'll get with a steamer.
Using heat from a hood dryer can also help 'activate' your conditioning treatment, but it won't add moisture. Plus, if your hair is not properly sealed with plastic or you stay under the dry heat too long, it can even dry out your hair.
Why deep penetrating moisture is so important to low porosity hair
Natural hair needs moisture because our strands' internal structure actually uses water for strength. Water also increases hair's plasticity; it makes it more flexible, which in turn makes it more manageable, easier to style and able to resist breakage.
Straight low porosity hair tends to take care of its moisture needs on auto pilot, especially when it comes to virgin hair. The different structure of curly hair makes holding onto moisture more complicated, so the hair's natural moisture needs to be topped up.
When porosity is extremely low, which happens naturally on many tight curly hair types, especially "4C", your hair can become so dry it breaks off. This happens because the natural moisture stores within the strand are not enough, and it's so hard to get moisture to penetrate with typical haircare methods and products.
Using steam is one way to overcome that barrier and allow these hair types - which are prone to breaking off before they hit their terminal length - to grow long. Add to that the fact that when your hair's moisture level is correct, it also feels a lot softer and smoother to the touch, and just more fun to play in.
How steaming helps increase your hair's moisture level faster
If you're trying to correct your moisture protein balance or get your hair moisture trained, a steamer, with its higher energy water molecules, is a much faster way to hydrate your hair.
A weekly steam will mean your hair (provided it is not extremely damaged) gets to its ideal moisture level within 1-2 months, as opposed to several months if you try to achieve this by weekly treatments with a typical deep conditioner and no steam.
How much does a good hair steamer cost?
Good steamers aren't cheap. A decent steamer will cost you in the region of £150, and the price can get a lot higher the more premium the model you're looking at.
How to get the benefits of steaming WITHOUT a hair steamer
If a steamer is sounding like exactly what your hair needs, but the cost is prohibitive right now, don't worry. It turns out you can get almost all the benefits of steaming if you just switch to a conditioner that penetrates better, and change the way you apply your conditioners.
OK there's no question: adding steam to this improved conditioning method would take your moisture levels up to stratospheric. But if you're looking for a moisture increase that's equivalent to the level that steam + any decent treatment will bring, then upgrading to a stronger treatment + this method will do the trick and then some, with no steamer needed.
The trick is threefold: first, it's about finding the right conditioning treatment for your low porosity hair, then learning how to apply it to low porosity hair so it absorbs, and thirdly making sure the timing is correct. Getting all these three things right will give you dramatic results. Here's how you do it:
Step 1: Pick the right treatment
f you want moisture at a steam type level, you need to use a conditioning treatment, not a regular conditioner. You can use rinse out conditioner as an additional product, but you'll need to use a hair mask, too. Treatments or hair masks are designed to be more intensive, which is what you need to get anywhere near the effects of steaming.
And don't just throw on any conditioning treatment and hope for the best. Go for a superconditioner if you want to replicate the work a steamer does and then some. Superconditioners are extremely concentrated, high performance conditioning treatments. Because they contain many times the typical level of conditioning ingredients, they feel a lot richer and can hydrate your hair way faster and on a deeper level than even good quality conventional deep conditioners.
How to identify the best treatments for steaming hair
You'll know these superconditioners by their thickness, and their higher concentrations of the ingredients that count. These are the ingredients that hydrate your hair, strengthen it and smooth it, by penetrating or adsorbing onto to your hair.
Ironically, the ingredients that do this hard work generally aren't the botanical extracts and natural oils most brands boast about on their labels.
Instead, look for actual conditioning agents high up on the list: cetrimonium chloride, cetyl alcohol, stearyl alcohol, to name a few. Silicones have gotten a lot of negativity in the media, but having one or two somewhere in the middle of the ingredients list is usually good news for your hair, too.
Also avoid very oily or buttery conditioning treatments when steaming low porosity hair. If there's too much oil or butter in the treatment, these ingredients can act as barriers, especially on low porosity hair which has limited room on the surface or inside the strand.
Finally, the treatment you choose should be very slippery to the touch. This characteristic, known as detangling slip, means the product has good movement along the strands. Not only does this help with combing more easily, it's also a sign of good coverage, which is essential for proper conditioning on low porosity hair.
For treatments that tick all these boxes, atrActiva Multivitamin, La Aplanadora Treatment and Halka Baba de Caracol are some good options. We list quite a few more here.
Step 2: Apply your conditioning treatment the right way
Once you've picked your treatment, it's all about the application. We'll be blunt: you need to be generous. Don't bother trying to get away with using just a teaspoon or a quarter size on low porosity hair, especially if your hair is very dry. Give your hair what it needs.
The goal is to take your hair right to the point where it's seaweedy with conditioner. That feel signals that your hair has started to absorb the treatment. We have a step-by-step guide on how to get products to penetrate into low porosity hair that explains exactly how to do it.
Then, take some plastic wrap and cover your head. Make sure all the hair is in, including the edges, and wrap it around your head a few times.
Step 3: Get the timing right
Leave your treatment on at least 20 to 30 minutes to allow it to truly take to your hair, then rinse it all the way out with warm water. If you've never ever steamed your hair, chances are you've never experienced your hair like this before. If you have steamed, then you know what to expect: intensely silky, soft, flexible hair unlike anything your regular rinse out conditioner, hot oil or deep conditioner can do alone on low porosity hair.
With a superconditioner and this intensive application method, you can consistently achieve steamer-fresh hair on low porosity hair without needing a steamer.
This method is so good to your hair, in terms of softness, smoothness, shine, manageability, growth and potential for definition, it's worth doing this every week to keep your hair in this pristine condition that is unlike anything you knew it could achieve.
This method will work especially well if you have 'hard hair' aka super low porosity hair that no conditioner, treatment or method ever seems to work on. To get the results, you need to follow every step to the T. If you have any questions on getting the steps right, just let us know in the comments section below.
The Super Low Porosity Survival Kit
We're putting the finishing touches on our first batch of the DHA Super Low Porosity Survival Kit. It's a compilation of the products that really work on super low porosity hair - the most extreme kind of low porosity hair out there - and a free course on how to care for this hair type. You can preorder your kit via the link below. Press the pink button for details.
DHA Hair Care Experts


text:
On May 6th, I will read the first pages of my unpublished manuscript in front of a group of industry professionals at the Festival of Literary Diversity in Brampton. The reading will be from Loved Mars, Hated The Food.
Writer’s Court is a live American-Idol-style event. I’m hoping I can make an impression with industry professional judges Max Arambulo, Dimitri Nasrallah, Adria Iwastiuk, and Janice Zawerbny. Hopefully, it will be a favourable impression.


text:
Subscribe and receive a 4L Casserole Dish
Plus, save 42% on your magazine subscription
To make the salsa, tip the beans, peppers, avocado and spring onions into a bowl. Season, dress with the olive oil and lemon juice, and set aside.
Lay the chicken breasts on a board and bash a bit to flatten them out slightly. Place in a dish, drizzle with a little olive oil, then coat with the Cajun seasoning. Heat a griddle pan and cook the chicken for 5 mins on each side, turning if you want to get a criss-cross pattern, until cooked all the way through. Stir coriander through the salsa just before serving. Serve the chicken either whole or sliced.

domain: food


text:
Arrow Earrings with Wire and Glass or Stone Beads - Final sale
These arrow earrings will hit their target! Made from hand-twisted aluminum wire, these earrings feature a glass or stone bead hanging at the bottom. They measure 2" in length. These earrings look great with their matching Sword Pendant Necklace.
This fair trade item is handmade by the Elder Center Bead Group, a group of young artisans in Santiago Atitlan, who earn a fair wage for their work. The profits from this product go to the Elder Center, which provides a place of community, meals, and healthcare services for 65 elders in Guatemala.
This item is final sale


text:
It doesnt take but a few minutes to recognize that Mara Getz is an artist of unique and seemingly limitless talent. She has an amazing versatility and a distinctive musical style that can range from recording studio work to intimate jazz clubs to broadway stages to the largest European music festivals without missing a beat. A 10-year industry veteran, Getz successful career has spawned a chart-topping solo album, honors in National and International music competitions and a loyal legion of fans around the world.
Getz career began singing in church at age five where her mother acted as choir director...and she nurtured her talent for singing and acting at many family gatherings while growing up. Wanting to make singing her livelihood, she packed her bags and moved to Los Angeles in 1982.
Within no time Getz was headlining in the local club circuit. Her local acclaim brought her to the attention of talent executives and she soon was appearing on "The Merv Griffin Show" and "You Write The Songs." Americans first recognized this farm girl from Baker, Minnesota as a 1985 finalist on TVs "Star Search."
Since then, she has headlined her own shows at clubs across the country as well as opened for such legendary entertainers as Bill Cosby, Joan Rivers and George Burns. The summer of 86 brought Getz first theatrical debut at the Matrix Theatre in Amanda McBrooms "Heartbeats." This singer/actress won the International Song Competition in Finland and Ireland and at the Sopof 86 International Festival in Poland she won the Grand Prix, receiving the top three honors of the Festival, becoming the first American to have done so in 20 years.
In 1988 Getz landed the lead female role in the musical "Mail," which played for a smash season in Los Angeles and then moved on to New Yorks Broadway stage. Soon afterwards, Getz was approached by Japans premiere record label, Pony Canyon Records. In 1989 her debut album, In Your Eves, was released in Japan, climbing into the Top 20 on the international charts as the albums title track bulleted to #3 on the singles chart. Her sold-out tour throughout Japan last June was a testimony to the excitement and popularity the album has created for her.
"Every once in a while, I have the opportunity to experience a thrilling, exciting new talent with unlimited potential. Mara Getz is among that small number of performers destined to climb to the top of the music business. She has the voice, she has the presence and she has the looks. Remember that name: Mara Getz. Youll be seeing it on album covers, concert posters and record charts very soon," said Back Stage magazine.
"Im going wherever my path takes me..whatever lessons I have to learn...music will always be the expression I choose" said Getz. Today, she has evolved into one of the clearest, purest, most poetic voices of the 90s. She is currently writing and producing her second album and she continues to bring her talents in both stage and concert appearances to her growing worldwide audiences. And when this woman gets a map in her hands, take heed. You never know where shell take you. So if youre ready to embark on an adventurous and visionary journey down uncharted musical pathways...one thing is for certain, Getz knows where shes been and where shes going. The road to the top should be a short one indeed.


Manufacturers these days have managed to generate economical, miniature GPS monitoring units. These minimal trackers are excellent for the whole host of responsibilities all around the house and ensure it is very simple to maintain a watch on in which your factors are heading.
The tracking machine has a GSM sim card that when it’s got credit on means that you are in a position to examine its whereabouts 24 several hours on a daily basis. You merely should dial its variety along with the chip will relay a message telling you its current longitude and latitude coordinates. This will either be entered into pc computer software including Google Earth or else you can subscribe to an internet based provider provider.
These moment monitoring systems have an in-built lithium battery which has a standard cost lifetime of a few times and then needs to be recharged. They can be precise to inside of a metre so are not challenging to find the moment moved in addition to occur while using the functionality of like seem to permit for audio surveillance if required.
Miniature house GPS trackers will also be equipped along with the capability of geofencing, which means that ought to they be taken outside a predefined area they’ll concern a warning. They can also be equipped by having an emergency, worry feature that will advise a central issue if there is an issue, for example a motor vehicle breakdown or theft.
How can these types of MI5 surveillance devices be of use towards the average house? Effectively in the course of a time of greater insurance coverage charges these devices will let you to maintain a watch on the residence, cars and valuables. Coverage corporations can also be probably to supply discounted rates if you will be able to monitor your most costly merchandise.
They are accustomed to manage connection with small children and elderly family members. In an ever-fearful earth, it’s great to grasp exactly exactly where your kids are at anybody time. Senior household members will also be a be concerned at times and these GPS tracking units can get rid of these worries by providing you suggestions of their locale. Home GPS trackers are little, light and unobtrusive indicating the individual carrying the GPS won’t be hindered by its existence.
See This Site Balise GPS