Take a long, sturdy metal pipe. Weld a cap over both ends, leaving only a small hole. Squirt in a bit of alcohol. The boiling point of fluids (such as alcohol) drops when the air pressure drops. Hook a vacuum pump to the hole, and suck out the air until the pressure in the pipe is so low that the alcohol will boil at the freezing point of water. Then seal the hole.
Toss the pipe into a lake or ocean. It will float on its side. Add weights to one end so the pipe will stand vertically in the water, with half its length sticking up into the air. The pipe is now a spar buoy. This is a very stable platform suitable for mounting scientific instruments.
In winter, when ice starts forming on the ocean or lake, the top of the pipe will get colder than the freezing point of water. Droplets of alcohol will form on the inside of the top end of the pipe, and will drip down to the bottom end of the pipe. The bottom of the pipe will be in relatively warm water, which will warm the alcohol droplets until they boil. A steady trickle of liquid alcohol will stream down the inside of the pipe, and a steady gust of alcohol fumes will waft upwards through the pipe. The entire length of the pipe will stay at the freezing point of water, no matter how cold the air gets above the water's surface. This is a heat pipe.
Now, take thin sheets of several dissimilar metals and weld them together into a sandwich. This will make a thermoelectric generator. Thermoelectric generators use temperature differences to make electricity, with no noise or moving parts. They're not very efficient, but are great for when you need a zero-maintenance power source. Stick this metal sandwich on top of the spar buoy. Stick big metal fins on top of the metal sandwich. The cold winter wind will chill the top of the sandwich, while the buoy will warm the bottom of the sandwich. This will generate a small amount of electricity.
You can then use this electricity to power any scientific monitoring equipment mounted on the buoy. This would be useful for long-term monitoring of arctic sea ice drift patterns, local weather conditions, and acoustic signatures of sea life and ship/submarine propellers. The buoy could constantly transmit its location and telemetry without worrying about exhausting its batteries.
You could anchor thousands of these in deep water near a small community, run wires down the anchor cables and along the sea bottom to the shore, and provide a modest amount of electricity. Areas with heavy sea ice wouldn't be good for anchored buoys (the ice would either crush a tethered pipe, or else rip its anchor and wiring off the sea floor). That rules out some more isolated communities most dependent on flown-in generator fuel, such as ᑲᖏᖅᑐᒑᐱᒃ. Communities with sheltered bays or freshwater lakes would be more suitable - perhaps ᖃᒪᓂᑦᑐᐊ, or (further south), Moosonee or Attawapiskat.


Will recreate music if it isn't too commonplace: Arjun Kanungo
"There are only so many hit tracks from the past that you can remake," Kanungo said.
New Delhi: Singer Arjun Kanungo has been quite vocal about not being in favour of remixes. However, he wouldn't mind being a part of a recreation effort in the future, if it doesn't become too commonplace.
"It's a trend and it will eventually pass. I mean how many songs are you going to remake? There are only so many hit tracks from the past that you can remake," Kanungo told IANS.
Kanungo, who gained popularity with numbers like "Baaki baatein peene baad", "La la la" and "Khoon choos le", says he was offered to lend his voice for a recreation but he turned it down.
"I recently had an offer to do a remix by my own label, but I turned it down because I felt right now is not the time for me to do something like this. Maybe in the future I would try doing it, if it's not so common," he added.


Bellevue Brewing Company
A major new brewery has arrived in town, and just celebrated its grand opening. Scott Hansen, founder of Leavenworth Biers, and real estate guy/beer enthusiast John Robertson founded the new brewery, taproom, and bar.
Was it intentional that Maria Hines's newest venture opened on the eve of doomsday? Ballsy. Well, we’re all still here and ready to tip our hats to southern Italy and Sicily at her third restaurant, located in Fremont.
Former Vios Cafe manager Jim Goodall is about to open his own deli of sorts. Eater Seattle says the menu will be perfect for picnics with soups, salads, and sandwiches available for takeout. Goodall hopes to open at the end of this month.
Seattle needs another gluten-free bakery...I’m serious. News of this venture broke earlier this week, now partners Phebe Rossi and Amanda Bedell just need to find a storefront for their bakery, potentially in either Capitol Hill or Wallingford.
In case you missed it, Darlene Boline is opening Zaccagni's, an Italian-style roasted sandwich shop in Pike Place Market. Meat is the main idea, with vegetarian options as well. Fingers are crossed for the beginning of January.
Did you also go through withdrawals when the pastaria temporarily closed to move locales? Well, the wait is just about over. Il Corvo will be opening New Years Eve for a special six-course dinner, but then really opening on January 7. Start your countdown now.
Capitol Hill Seattle blog spoke with Aissa Diouf, owner of Saley Crepes, about her plan to move from 1650 E Olive way to new digs just a few blocks away, near 8th and Olive. Her reason? A better kitchen space for her sandwiches and crepes.
Dirty Bucket Brewing
Woodinville Patch says that the young brewery has done so well its first months that an expansion is already in the works. Attributing the early success to the support from patrons, the Bucket crew plans to purchase more brewing equipment as well as the additional space next door. The winter ale was just released yesterday.
That rumored happy hour starting at the new Armory? Queen Anne View says we’ll have to wait another month until February 21. But it’ll be worth it with these powerhouse restaurants on the list.


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The Baton arrives today (09-12-2013) in Tonga.
Named the Friendly Isles by Captain Cook as a result of the congenial reception accorded to him and his crew on their first landing there in 1773, we look to the International Heritage project for our connections with that country.
The University’s Special Collections holds a copy of James Cook, A voyage towards the South Pole, and round the world. (London: printed for W. Strahan & T. Cadell, 1777, vol. 3, plate XLII), in which the illustrations of William Hodge includes boats of the Friendly Isles.
Other artifacts from Tonga are held by The Hunterian and include an octopus lure (GLAHM E.176) and a head rest or ‘sleeping stool’ (GLAHM E.441/3):
– you can read further information on these collections here.

domain: geography


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One of the best parts of starting this blog for you is the things we learn during the process. One example, the art of fabric design is truly global. So far, we’ve featured designers from Australia and the United Kingdom. Now, our latest designer spotlight shines on trailblazer Emma Schonenberg, from El Salvador. In fact, Schonenberg is the first and only designer from that country to export and sell her creations abroad. How awesome is that?!
Schonenberg spent some time working for companies in advertising, apparel, home decor and USAid funded programs in El Salvador and in Iraq. About 15 years ago, she discovered surface pattern design by chance through scrapbooking. “I started out designing collections for this industry not really knowing what I was doing, just dreaming I’d get a paper company that would purchase my work,” Schonenberg said.
After showing her designs off at trade shows, doors began to open in the textile industry. And the rest, as they say, is history. “Taking these chances led me to this amazing world I love so much! So this experience with Michael Miller was a lovely, lovely treat!” Schonenberg told us.
That experience with Michael Miller includes the “Be Kind to Everything that Grows” panel you can see here. One look at that pattern and it’s easy to see where Schonenberg draws inspiration from. “I find inspiration everywhere. I can find it in even things like an aged tarp covering wood at my sister & brother in law's home. But nature mainly. She is my generous muse! I get lost in all it offers! When we look closely, oh my God! Speechless!” said Schonenberg.
A quick browse of Schonenberg’s work proves her point. Nature plays a major role in her designs, and has helped lead to even more opportunities for her. She explains when asked what her favorite design is. “My soul gardens, as I fondly call them. Drawing these have helped me stay grounded, let go of perfectionism and really start loving all my lines. The feeling I get when I am working on them truly fills my heart. My love for them is so much that it took me to get a masters in Art Therapy and am a few months away from launching a Therapeutic Art program for anxiety relief. Soul Gardens hold that special place in my heart. Michael Miller picked up on them and did a full collection with them.”
As for aspiring designers, Schonenberg offered this priceless piece of advice. “People buy art, yes, but they also buy that feeling found in our art. Our voice contains our soul and it comes through our art. Finally, don’t be shy. The world needs you and your art. Clients love to get to know you and besides, you have so much to win!”
Certainly words to live by. Thank you to Emma for taking some time out of her busy day to answer our questions.
And as always, Happy Quilting!

domain: artistic


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Factors Influencing Information and Communication Technology Implementation in Government Secondary Schools in Kuwait
Alajmi, Saad Shaji Saad
Thesis or dissertation
University of Exeter
Over the last few decades, Information Communication Technology (ICT) has become increasingly important in all organisations in modern societies including schools. Expectation about ICT and its role in developing and transforming the educational process through its inclusion in the daily school environment is increasing in many developed and developing countries. Many researchers and practitioners are involved in exploring and investigating ICT implementation in the learning and teaching process in secondary schools. This research explores and investigates influential factors and barriers surrounding ICT implementation in government secondary schools in Kuwait from the perspectives of students, teachers and expert educators. The study is executed through two phases: an exploratory pilot study, (see Appendix 11), and this deep investigating study. The exploration and investigation process of this study focuses on six main areas: the ICT policies and strategies in the Ministry of Education, the readiness of school environment for ICT implementation, teachers' views regarding ICT, surrounding socio-political factors, other main barriers to ICT implementation and, finally, the main requirements for better ICT implementation. The research has adopted a sociocultural approach whereby research problem has been explored and investigated through different individuals in different contexts that are related directly or indirectly to ICT implementation in government secondary schools. Data, as well, has been collected through a wide range of probing methods such as documents, self-managed questionnaires and interviews in order to answer the research question. The pilot study explores the research situation through 52 students and four teachers and in this main study 306 teachers were investigated through questionnaires and 15 teachers and expert educators were interviewed. The findings indicate that Government of Kuwait and the Ministry of Education have a strong intention to implement ICT in secondary schools and have already executed many related practical steps. The study also reveals that most teachers have positive views towards ICT. However, findings also show that the school environment is not yet ready for ICT implementation and that policy and decision-making processes are suffering from a lack of clear vision and instability. The surrounding socio-political factors have a negative impact on educational policy in general and ICT policy in particular. This research also identifies some other barriers to ICT implementation and, finally, highlights some requirements for improving the implementation of ICT.
PhD in Education

domain: literature


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Flu Vaccine Availability
Flu vaccines availability (updated 11/13/13): Injectable flu - available for ages 6mo to 36 mo only Nasal spray (Flumist) - available for ages 2 yea.....
What is the Flu?
What is the flu? The influenza (flu) virus causes serious illness that may result in hospitalization or death. It mostly affects the breathing system.....
Dietitian at Williamsville Pediatrics
There is an exciting program through NYS Dept of Health (WNY Project for Pediatric Obesity Prevention) that we have the privilege of partici.....
If You Choose Not To Vaccinate
If you choose not to vaccinate, please read the following so that you understand the risks of your decision. If You Choose Not To Vaccinate.....
Important Office Policies
Williamsville Pediatric Center would like to notify you about policy changes in our office. Clinical Visit Summaries (Updated 5/17/13) .....
Come Grow With Us!
WHO WE ARE:
PEDIATRIC GROUP PRACTICE - Our team of highly regarded Board-Certified Pediatricians and Nurse Practitioners are committed to providing exceptional health care for your children. Pediatrics is our passion - come grow with us! Located in Williamsville, a suburb of Buffalo, New York, the physicians at Williamsville Pediatrics serve the Western New York pediatric needs of the communities of Williamsville, Clarence, East Amherst, Amherst, Depew, Lancaster, Lackawanna and beyond. It has been our pleasure to deliver acute and preventive pediatric care at the highest level since 1989.
LEVEL 3 NCQA CERTIFIED PATIENT-CENTERED MEDICAL HOME
NCQA’s Patient-Centered Medical Home program highlights primary care providers who have shown us they can coordinate care among all a patient’s doctors and other health care providers. You get better access through expanded hours. You get help understanding treatment options and are a partner in decisions about your care. You have better access through expanded hours and the web. Providers work in teams to prevent problems and manage chronic conditions to keep you healthy. The result is better quality and fewer costly trips to emergency rooms and hospitals.
NCQA Patient-Centered Medical Home standards show primary care providers how to make these important changes. There are three levels of Patient-Centered Medical Home recognition. Level 3 is the highest level. Click here for more information.
PRIMARY CONNECTION SITE
BREASTFEEDING FRIENDLY PRACTICE
Williamsville Pediatrics is proud to be one of the first practices in New York State to be pursuing the designation of "Breastfeeding Friendly Physician Practice".
PATIENT PORTAL PARTICIPANT
Patient Portal is now active! Watch this video on patient portals.

domain: medical


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Pro abortion debate essays
In the era of political polarization, it appears that almost any subject matter can spark controversy and debate abortion is no exception to this rule: therefore, contrary to popular media portrayals of the debate between pro-life and pro-choice groups. Pro abortion debate essays on education, accounting homework help for college students, does a good thesis statement help guide the rest of your paper 25 lut, 2018. 1 sample essay #11 abortion is the most controversial issue having no grounds of agreement among two polar aspects the argument is life and death though. Abortion q&a essays abortion rights are pro-life abortion: a moral choice contact select page essays abortion rights are pro-life by leonard peikoff roe v wade anniversary still finds defense of the right to abortion in case of rape is an argument texas democrat attempts to. Abortion is a heated debate in the us, filled with emotion and lacking rational thought this infographic explores quantifiable data to help readers understand both sides.
You have considered both sides of the argument before choosing your position 2) you are able to anticipate and refute any opposing arguments before you organize your essay pro: abortion should be legal con: abortion should not be legal ever. The most common skewed argument against abortion is: you are killing a baby what is being eliminated is not a baby persuasive essay: pro choice the horrific photo of geraldine santoro has grasped the attention of the media and society. Pro abortion debate essays on success, university of utah creative writing online, creative writing journeys stimulus the struggles of getting lead smeared on your hand while writing an essay left handed. Your thesis should be an argument or statement followed by three reasons that support that argument for instance abortion is justified because ____, ____, and ____ if it is just a researched essay abortion, in the context of pro-choice versus pro-life. Pro choice abortion arguments essays zoning map, father of the bride speech writing service, resume writing service reviews 2017 be social chick categories.
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Pro abortion debate essays
Pros and cons of abortion this research paper pros and cons of abortion and other 63,000+ term papers, college essay examples and free essays are available now on reviewessayscom. Pro abortion essay pro abortion essay argumentative essay topics on abortion highlight the problem in terms of pro-life arguments (against this procedure), and pro-choice arguments (for legalization. Free abortion debate papers, essays, and research papers.
Abortion pro and cons essays one of the most important issues in the world is the controversy surrounding abortion do you think abortion is right many people have been asking themselves this same question for years now abortion has been a heated argument amongst citizens, pol. Bad pro-choice arguments abortion is a very controversial subject in our country and rightly so opponents of abortion charge that it is the murder of an unborn child. Included: abortion essay content preview text: abortion has been one of the biggest controversies of all time many people believe it is immoral and even consider it to be murder the definition of abortion is. Do you need assistance with writing a 5-paragraph argumentative essay on abortion topics the following essay writing tips provided by our writers will help you for sure our service pricing the debate on whether abortion should be legalized has dominated discussions for years. An introduction to the abortion debate, setting out the major questions involved in the matter of terminating a pregnancy.
Free essay on pro choice abortion essay available totally free at echeatcom, the largest free essay community. Free argumentative essay on abortion, pro-life this paper examines the contentious issue of abortion abortion, pro both sides in the debate present valid arguments to either support or reject abortion pro-life arguments are mainly based on the concept of preservation of human life.

domain: politics


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On the web mods mean fallout 4 adult mods happened on a PC or PC framework on the Game gaming framework or even a PC. You have games inside their least difficult compose, depending on instant message focused circumstances to individuals enormously multi-player games online with online universes, delighted in all the while with a crowd of players. The astonishing ascent in ubiquity of the online fallout 4 adult mods has developed to this sort of level with respect to promote even on-line neighborhoods. The most continuous games online are Gaming framework gaming like Game 360 Support First Individual Shooter courses like Demise coordinate precisely where various competitors involve in bloody battle situations Ongoing Strategy applications using a neighborhood gathering to encounter multi-player game titles Cross Framework Online Play exhibited web based gaming’s development, with web based games like Ps 2 and Nintendo Block remain for fundamentally message based multi-client bona fide time online universes Incredibly Multi-player Games utilized some extraordinary advantages of the broadband Web to empower of members to partake in a game title at the same time.
Most rumored games online fit into the manager expert of EULA or perhaps the End Client Permit Course of action, incorporated into on the web administration. Breakdown to pursue their contract will welcome irritating results. Terrible activities as badgering and digital tormenting all through the game and in gab are standard alternatives that accompany these internet games.
Late examination proposes that web based games have a section to play in enhancing the player’s head. The genius adolescents are over a standard chase to discover how each and every little subtlety in the on the web adult game could alter the player’s propensities, awareness and capacities. A considerable measure of specialists confirm a positive change in their general execution and highlights which incorporate such things as advancement inside their night voyaging. Also, they derive that standard earn v-bucks in fortnite upgrade their basic leadership approach by more than 25 Percent, allow them to make quicker options and act about them 4x more rapidly than expected people. These free examinations additionally exhibited a stamped change in center and retentive capacities. On-line Games have said some unfavorable outcomes in examination achieved on young fellows who are playing rough games. They guaranteed that scarcely multi week into these sorts of plans seemed to affect people parts of the young men’s brains, which took care of sensations, connected to real wretchedness. Urgent video gaming demonstrates signs of being overweight, despondency and an inclination to end up being a self observer.

domain: game


text: After a mid-week hiatus from our field work, we got back at it again today. Our goal was to finish the last couple transects upstream of the Uintah bridge. We did complete our goal by the end of the day, however it did not occur without a hurdle. Near the end of us surveying transect 13, we heard rolls of thunder coming just over the Front Range. Utilizing the extra energy in the air, we quickly finished up and packed our cart full of equipment and hunkered down underneath the bridge. After much deliberation of whether or not we would try and run back before the rain hit, the rain hit. We stayed put. Luckily the rain did not last long, so we got back out there and completed transect 14.


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Do you love salmon but dread the idea of having to cook it? Cooking salmon to perfection can be a daunting task, but it doesn’t have to be. Have you tried cooking frozen salmon in an air fryer? If not, you’re missing out on a juicy and delicious meal that’s easy to make. Using an air fryer to cook frozen salmon is a game-changer when it comes to meal prep.
It’s quick, easy, and produces a crispy exterior while leaving the inside moist and tender. Not only that, but it’s also a healthier alternative to traditional frying, as it uses little to no oil. To ensure that your frozen salmon turns out perfect, there are a few tips and tricks that you should know.
From preheating your air fryer to seasoning your salmon, we’ve got you covered. In this blog post, we’ll take you through everything you need to know about cooking frozen salmon in an air fryer. Whether you’re a beginner cook or a seasoned pro, cooking frozen salmon in an air fryer is a great way to expand your culinary skills.
With our tips and tricks, you’ll soon be able to cook up a delicious and healthy dish that will impress your family and friends. So, grab your air fryer and let’s get cooking!
Yes, You Can!
Yes, you can put frozen salmon in an air fryer! In fact, cooking frozen salmon in an air fryer is a great way to save time and effort. The air fryer’s high-speed circulation of hot air helps cook food quickly and evenly, making it perfect for frozen salmon. To do this, simply preheat your air fryer to 375 degrees Fahrenheit, place the frozen salmon in the air fryer basket, and then let it cook for about 8-10 minutes.
Don’t forget to check the salmon halfway through cooking to ensure that it doesn’t burn. Once the salmon is done cooking, take it out, and enjoy a delicious and healthy meal that can be ready in minutes. So, whether you’re short on time or just looking for a convenient way to cook salmon, the air fryer is the perfect solution!
Air Fryer Works Great for Frozen Salmon
If you’re a salmon lover, you’re probably wondering if an air fryer is a good way to cook your frozen salmon. The answer is a resounding yes! An air fryer is a perfect solution for cooking frozen salmon to perfection. Not only does it cook the salmon quickly, but it also locks in the moisture and flavor, so you end up with a delicious result.
Just place your frozen salmon fillet in the air fryer, add your desired seasoning, and within minutes, you’ll have a tasty and healthy meal. Plus, cooking with an air fryer means no messy cleanup afterwards, so you can enjoy your meal without worrying about the aftermath. So, the next time you have frozen salmon in your freezer, don’t hesitate to pull out your air fryer and give it a try.
You won’t be disappointed!
Saves Time & Effort Preparing Salmon
Salmon is a delicious and nutritious fish, but preparing it can be a time-consuming and daunting task. However, with a few simple tricks and techniques, you can easily save time and effort without sacrificing taste or quality. First, consider marinating your salmon to enhance its flavor and tenderize its texture.
You can use a variety of ingredients, such as soy sauce, honey, garlic, and lemon juice, to create a customized marinade that suits your taste preferences. Second, try cooking your salmon in foil packets or parchment paper to seal in the moisture and flavor. This method also reduces the mess and clean-up time.
Finally, use a meat thermometer to ensure that your salmon is cooked to perfection. This way, you can avoid overcooking or undercooking it and achieve a moist and flaky result every time. By applying these tips, you can save time and effort preparing salmon and enjoy a healthy and tasty meal.
Yes, it is possible to put frozen salmon in an air fryer. However, before placing the salmon in the air fryer, it is crucial that you properly prepare it. Firstly, you need to thaw the salmon completely before cooking it.
This can be done by placing it in the refrigerator overnight or by using the defrost function on your microwave. Once the salmon is completely thawed, you can season it with salt, pepper, herbs, or spices of your choice. Preheat the air fryer to 400 degrees Fahrenheit, and place the salmon skin-side down in the basket.
Depending on the thickness of the salmon, it should be cooked for 8-12 minutes, or until the internal temperature reaches 145 degrees Fahrenheit. Avoid overcrowding the basket, as this can affect the cooking time and possibly lead to unevenly cooked salmon. In conclusion, placing frozen salmon in an air fryer is completely doable, but make sure to fully thaw the fish and prepare it correctly before cooking.
Seasoning and Marinating Frozen Salmon
Frozen salmon often gets a bad rap because people are unsure about how to prepare it properly. However, with a few simple steps, you can transform your frozen salmon into a mouthwatering dish. To start, you should always thaw your salmon in the refrigerator overnight, as thawing it in the microwave or on the counter can cause it to lose texture and flavor.
Once thawed, you can add a variety of seasonings and marinades to elevate the flavor. Lemon, garlic, and dill are classic seasonings that go well with salmon, while a soy sauce and brown sugar marinade adds a sweet and salty kick. No matter what you choose to season or marinate with, give your flavors time to penetrate by letting the salmon sit for at least 30 minutes before cooking.
With these tips, you can turn your frozen salmon into a culinary masterpiece that will leave your taste buds singing!
How to Preheat Your Air Fryer
Air Fryer When it comes to cooking with your air fryer, preheating is an essential step that you don’t want to skip. Preheating your air fryer helps to ensure that your food cooks evenly, giving you that perfect crispy texture that we all know and love. Preheating is a super simple process and involves nothing more than setting your air fryer to the desired temperature and letting it run for a few minutes before adding your food.
In general, it’s recommended to preheat your air fryer for around 3-5 minutes, but this can vary depending on the model and the specific recipe you’re using. By taking the time to preheat your air fryer, you’re sure to get consistently delicious and perfectly cooked food every time. So next time you fire up your air fryer, make sure to give it a few minutes to preheat, and you’ll be amazed at the results!
The Cooking Time and Temperature
When it comes to cooking, the preparation method is just as important as the ingredients themselves. Different methods require different temperatures and cooking times to achieve the desired outcome. For example, roasting typically involves high heat for a short period of time, whereas braising involves low heat for a longer period of time.
It’s important to pay attention to these details to prevent undercooking or overcooking your dish. Additionally, the type of equipment you use can also affect the cooking time and temperature. A gas stove may require a different setting than an electric stove, and an oven may need to be preheated to a certain temperature before cooking.
By taking the proper steps to prepare your ingredients and adjust your cooking method accordingly, you can ensure a successful and delicious meal every time.
Are you wondering if you can put frozen salmon in an air fryer? The answer is a resounding yes! Air fryers are perfect for cooking frozen salmon as they circulate hot air around the fish, thawing and cooking it simultaneously. To cook frozen salmon, simply preheat your air fryer to 400°F, season the salmon with your preferred flavors, and place it in the air fryer basket. Cook for 12-15 minutes, flipping the fillet halfway through.
You’ll have perfectly cooked salmon in no time! Other serving suggestions for air fryer salmon include making salmon tacos, salmon burgers, or topping a salad with flaked salmon for a protein-packed meal. So don’t be afraid to experiment with frozen salmon in your air fryer and enjoy a quick and delicious meal.
Pairing Frozen Salmon & Air Fryer with Delicious Sides
Pairing frozen salmon with an air fryer can be a game-changer when it comes to creating a delicious and healthy meal. When preparing salmon in the air fryer, you can easily add various seasonings and sauces to enhance its flavor. Some great serving suggestions include a side of roasted sweet potatoes, crispy Brussels sprouts, or a fresh garden salad.
For a burst of flavor, you could try a drizzle of lemon or balsamic vinaigrette. Not only is this meal quick and easy to prepare, but it is also a great source of protein and healthy omega-3 fatty acids. Give this simple yet satisfying meal a try and impress your taste buds with a nutritious and delicious feast.
Easy and Healthy Air Fryer Frozen Salmon Recipes
When it comes to serving up your air fryer frozen salmon, there are a variety of options to choose from. You can serve it with a side of steamed vegetables and brown rice for a healthy and balanced meal. For a bit of added flavor, consider making a simple herb butter or lemon dill yogurt sauce to drizzle over the top.
If you’re looking for something a bit more decadent, try serving your salmon on a bed of creamy mashed potatoes or pair it with a flavorful risotto. Another fun option is to create salmon tacos or a salmon salad, using your air fried salmon as the star ingredient. No matter how you choose to serve it, air fryer frozen salmon is a quick, simple, and healthy option that is sure to satisfy your taste buds.
If you’re ever in a hurry and forgot to defrost your salmon, the air fryer might just save the day. Cooking frozen salmon in the air fryer is actually possible, and it’s a great way to make a quick and easy meal. However, it’s important to note that the texture and flavor may differ slightly from fresh or thawed salmon.
When cooking frozen salmon, be sure to increase the cooking time and temperature to ensure that it’s cooked thoroughly. But don’t worry, your air fryer can handle it! Just keep an eye on the salmon and use a meat thermometer to ensure it’s cooked to the appropriate temperature. So, to answer the question: Yes, you can put frozen salmon in the air fryer.
Just remember to adjust the cooking time and temperature accordingly and enjoy your delicious, hassle-free meal!
In conclusion, the answer is yes, you can put frozen salmon in an air fryer. However, before you do, there are a few things to consider. Make sure to properly thaw the salmon beforehand for even cooking and to avoid any potential safety concerns.
Additionally, don’t be afraid to get creative with seasonings and cooking times to achieve the perfect crispy yet tender salmon. So go ahead, give it a try and delight your taste buds with a healthy and easy meal!”
Is it safe to air fry frozen salmon?
Yes, it is safe to air fry frozen salmon. However, it is recommended to thaw the salmon before air frying for the best results.
How long should I air fry frozen salmon for?
It depends on the thickness of the salmon fillet. As a general rule, air fry for 10-12 minutes at 375°F, flipping halfway through the cooking time.
Should I brush oil on the frozen salmon before air frying?
Yes, it is recommended to brush a little bit of oil on the frozen salmon before air frying to prevent it from sticking to the air fryer basket.
Can I season the frozen salmon before air frying?
Yes, you can season the frozen salmon with your favorite seasoning before air frying. It will enhance the flavor of the salmon and give it a delicious taste.

domain: food


Climate change politics burn hot after Green New Deal vote
WASHINGTON (AP) — Suddenly the climate is hot on Capitol Hill.
A day after the Senate blocked consideration of the Green New Deal, lawmakers from both parties were still talking about climate change. House Democrats pushed a plan to keep the U.S. in a global climate agreement, while Senate Democrats formed a special climate committee to match one in the House.
Republicans, meanwhile, continued to mock the Green New Deal as a step toward socialism and pushed for a House vote. The GOP launched a House Energy Action Team to counteract what they called the “radical” and potentially devastating Green New Deal.
The sheer volume of discussion made clear the plan has struck a nerve as both parties seek to use it to their advantage in the 2020 elections.


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- Color: Pink
- Dimensions: H 25.5 x W 7.5 X L 7.5 cm
- Diameter: 7.5 cm
- Weight: 360 gr
- Material: Stainless Steel
- Capacity: 500 ml
This drinking bottle is an absolute must! Not only is this drinking bottle beautiful to look at, it is also super practical, usable, and of perfect quality. You can use it for both hot and colds.
RICE is a Danish interior design and accessories company, known for colorful melamine, handmade baskets, lovely storage – items for living room, kitchen and kids room – all in happy colors and everything you need for your everyday life.


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Ignorant of their ignorance, yet wise
In their own esteem, these deluded men,
Proud of their vain learning go round and round
Like the blind led by the blind. Far beyond
Their eyes, hypnotized by the world of sense,
Opens the way to immortality.
"I am my body; when my body dies,
I die." Living in this superstition
They fall life after life under my sway.


Our wheel-side and gear-side constant velocity joints come with the relevant sealing system boot, clamps, bolts, nuts, seeger and locking rings. Our steel outer race, cage and inner race are manufactured and treated for maximum resistance to wear. We grind them to tolerances of hundredths of a millimetre so that together with high precision balls they guarantee regular and lasting performance. Most articles have an ABS ring made in a sintered material or forged steel.
Our range of ready-to-use driveshafts covers the aftermarket demands of the entire European car parc (including those of our OE customers). The components – wheel side joint, gear side joint, shaft and seal systems – are all already assembled. This means they’re quick and easy to install.
The kit consists of boot, clamps, bolts, nuts, seeger, locking rings and grease. We use two different materials for the boots – mostly rubber as it’s easiest to install and most reliable over time, but also thermoplastic, which is more resistant to wear and corrosion from aggressive agents.
This additional range complements the drivetrain line and completes the selection of products available to customers. The wheel bearing kit is a very important safety element asit has to withstand considerable axial and radial stress due to bends, kerbs, acceleration, landslides, rough roads and the weight of the vehicle.


Correctly labeling cannabis products for the amount of tetrahydrocannabinol (THC) and cannabidiol (CBD) is important for users, especially patients, to ensure that they are consuming the correct dosages. The challenge, however, is that chocolate cannabis products are making that a lot more difficult.
A recent study by Dr. David Dawson found that when chocolate is mixed with cannabis, it skews the THC levels. A notable finding from the study: "When we had less cannabis-infused chocolate in the sample vial, say 1 gram, we got higher THC potencies and more precise values than when we had 2 grams of the same infused chocolate in the vial."
The research was, unfortunately, unable to uncover the key ingredient in chocolate that was distorting THC levels to determine what other products might result in similar issues. For instance, further research is planned to look at chocolate chip cookies to see if there are similar problems uncovered there as well. It's important to note that this testing didn't look at CBD, as that may also be an area of concern.
Edibles are a challenge for the industry
One of the biggest dangers with consuming edible marijuana products is that often their effects, like the feeling of being high, aren't felt right away. Someone may consume an edible product thinking they can handle more since they don't initially feel the high. However, because it may take at least half an hour to notice the drug's effects, the danger is that during that time, someone may consume more and when the high sets in, they could be in for pain, discomfort, and panic. Overdosing on edibles can be easy to do, especially for new users who aren't aware that they have to wait to feel its effects.
Accurate labeling of these products is vital to ensure consumers know what they are consuming. Labels are important not only for recreational users, but also for medical marijuana patients so they know they are taking the proper dosages. Patients often prefer edibles to smoking dried flower because of the ability to more easily control their portions. Instead of trying to estimate how much was inhaled by smoking cannabis, it's a lot easier to count portions in terms of gummy candies or pieces of chocolate. The taste also makes it more appealing.
However, with more people going to emergency rooms as a result of consuming edibles, that's only going to become a bigger problem as more parts of the world legalize cannabis. That means it's going to be even more important for regulators like the U.S. Food & Drug Administration and Health Canada to stay on top of the industry to ensure that labels on products are correct.
One company that's investing in the chocolate edibles craze
OrganiGram Holdings (OGI -3.29%) is looking at the chocolate cannabis market. Earlier this year it spent CA$15 million on a production line that would allow the company to produce 4 million kilograms of chocolate cannabis products every year. It's for good reason, too, as edibles have been on the rise, making up 15% of the consumable cannabis segment in the U.S. in 2018. There's a lot more growth expected, with research firm BDS Analytics projecting that by 2022, edibles will account for $3.4 billion in sales. With chocolate being the third-most-popular category in edibles, behind tinctures and candy, that could mean a lot of growth for OrganiGram and other companies looking to produce similar products.
OrganiGram won't be able to tap into that growth in the U.S. just yet, but edibles could gain similar traction in Canada once the edibles market opens up before 2020. Once that happens, the company could be a big player in that segment of the overall cannabis market, potentially paving the way for entry into the U.S. market once it's legal to do so.
While many cannabis companies have set their sights on beverages and other edible products, OrganiGram has been one of the few to narrow in on chocolate, which could pay off. The challenge for the company will be how it deals with ensuring that its labels are accurate and that consumers are getting exactly what they ordered.


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Molinia caerulea ssp. arundinacea 'Karl Foerster'
More About Molinia caerulea ssp. arundinacea 'Karl Foerster'
- Forms a tufted upright clump of slightly arching, medium green leaves
- Open panicles rise well above the foliage
- Slender flower stalks gives the grass an airy appearance
- Long flower spikes change to tan seeds as they age
- Leaves turn a bright yellow in the fall
- Bloom Time: July
- Flower Colour: Purple
- Flower Height: 150 cm
- Foliage Height: 100 cm
- Grass Type: Cool Season
Karl Foerster Moor Grass Growing and Maintenance Tips
Molinia is slow to mature and could take 3-5 years to reach full size. Grows best in a moist, well-drained, acidic soil, in full sun to partial shade. You can trim back the dead foliage in the fall for a tidier appearance, or leave it until spring to improve winter interest and provide root protection.
Molinia caerulea ssp. arundinacea 'Karl Foerster' Characteristics
Moist to Wet Soils
Moist to Average
Landscape usesRooftop (extensive)

domain: biology


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Eating the recommended five servings of fruits and vegetables isn't always easy. Even if you do, you still might not be getting the wide range of vitamins and minerals you need. Each color group of fruits and vegetables offers different beneficial effects, so to get these essential nutrients, you have to eat the rainbow! What Color Is Your Smoothie?
helps you do just that. Inside, you'll find delicious, easy-to-make recipes, such as:
- Sparkling Strawberry Smoothiebright red strawberries are packed with vitamin C. They also contain heart-healthy omega-3 fatty acids and belly-filling fiber.
- Clever Carrots and Spiceorange carrots are full of beta-carotene, which helps prevent cancer, arthritis, and cardiovascular disease
- Pineapple Banana Creamyellow bananas are rich in carotenoids, which can decrease the likelihood of lung cancer
- Spicy Spinach Smoothiedark green veggies contain phytochemicals, which can protect your eyes by keeping your retinas strong
- Blueberry Blastoff Smoothieblue and purple foods contain flavonoids, which help prevent short-term memory loss
With more than 300 colorful, vitamin-packed smoothies to choose from, you'll blend, smooth, and shake your way to a healthy new you!


|By Chris Casteel, The Oklahoman, Oklahoma City|
|McClatchy-Tribune Information Services|
The bill, approved 251 to 166, now goes to the
Lucas, a rancher from
Wednesday, he said the "long and seemingly epic journey" had ended with legislation that improved the farm policies in place since 2008. The
Lucas and other supporters touted the elimination of controversial direct cash payments to landowners -- many of whom don't plant crops -- and the movement toward crop insurance as the primary safety net for farmers.
Besides crop insurance, the bill introduces new safety net mechanisms to protect farmers when crop prices drop.
Critics said those programs could wind up costing taxpayers far more than estimated.
"Any slight dip (in prices) will mean huge payments going out in the future," said Rep.
The large majority of the money in the bill goes to the food stamp program, which exploded in cost after the economic downturn in 2008.
Praise and criticism
Cole, who said he had 14,000 farmers and ranchers in his district, praised the bill for preserving American farmers' "capability to provide more food and fiber than any other country."
National trade groups for wheat, corn, soybean and cotton growers supported the compromise bill, but meat producers worked against it, complaining that lawmakers should have repealed the law that mandates country-of-origin labeling.
Lankford, who is running for the
The earlier House version had much deeper cuts to the
The measure approved Wednesday would cut about
Many Democrats opposed the bill because of the food stamp cuts.
"How can I explain the change is minor when they lose
Conservation groups praised several elements of the bill, including the requirement that producers who receive taxpayer subsidies for crop insurance premiums take steps to protect wetlands and prevent soil erosion.
(c)2014 The Oklahoman
Visit The Oklahoman at www.newsok.com
Distributed by MCT Information Services


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From Still Life to the Screen
Print Culture, Display, and the Materiality of the Image in Eighteenth-Century London
|Publication Date:||Oct 2013|
| Publisher:||Yale University Press|
| Book Format:||Hardback|
|List Price:||USD $85.00|
| Book Description: |
From Still Life to the Screen explores the print culture of 18th-century London, focusing on the correspondences between images and consumer objects. In his lively and insightful text, Joseph Monteyne considers such themes as the display of objects in still lifes and markets, the...


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First thing first, I’m going to announce you that on this post and the next 6 posts are going to be occupied with these ‘Seven Wonders of the Ancient World’ topic. It’ll not going to be long-winded, because I’m trying to make it concise, compact and tangible as possible.
Every day, people from around the world come to visit New York either by land, water or air transportation. Those who came to New York by water transportation will be greeted by the well-known huge female which is a gift from French, with its peculiar pose; lifting a torch, carrying books and wearing a crown. Indeed that’s the renowned Statue of Liberty, but some people called it the ‘Modern Colossus’ which is located near the Harbour. Some of the people don’t know that the word colossus itself reflects to another eminent statue which is called ‘The Colossus of Rhodes’, one of the Seven Wonders of the Ancient World.
The statue was used to be located in the Island of Rhodes (now modern Greece), off the south-western tip of Asia Minor where the Aegean Sea meets the Mediterranean, which was a cardinal economic centre in the ancient World. The island was used to be conquered by Mausolus of Halicarnassus in 357 BC before it fell into Persian hands in 340 BC and finally being crumpled by Alexander the Great in 332 BC. Lamentably, Alexander died young due to a certain fever, as the results, his generals, Ptolemy, Antigous and Seleucus, were fighting over his throne. However, the Rhodians were supporting Ptolemy in this fight which provoked Antiguous’s resentment by sending his son Dementrius in 305 BC to punish the city of Rhodes, or in other words, declaring a war.
The war was long and painful; over 40.000 men were sent in to the war, more than the population of the city of Rhodes itself. Dementrius sent a certain siege tower that was equipped with consummate and advanced armaments like for example catapults, water tanks to avoid flame throwers and some other equipment that are meant to be protecting the soldiers. After Ptolemy succeeded to tumble the second siege tower called the Helepolis, a massive structure with approximately 150ft high and weigh 160 tons, Demetrius withdrew quickly, leaving the great siege tower where it was. He signed a peace treaty and called his siege a victory as Rhodes agreed to remain neutral in his war against Ptolemy.
To celebrate their victory and freedom, the Rhodians agreed to build a colossal statue of their patron God, Helios. They melted bronze from those siege towers left by Dementrius for the scaffolding cardinal material. The architect of this great construction was Chares of Lindos, a Rhodian sculptor who was a patriot and fought in defence of the city. Chares had been involved with large scale statues before. The statue was one hundred and ten feet high and stood upon a fifty-foot pedestal near the harbour entrance perhaps on a breakwater. Some says that the statue’s leg pose straddled among the harbour entrance so that ships can pass though below, but some also says the statue was located near the harbour, standing alone gallantly, more like in a common pose manner for a Greek God’s sculpture. Approximately this statue was finished built for 12 years, and some people said that Chares, the architect, did not have the chance to see his own masterpiece, some says due to suicide (however we could not make sure since we don’t have the bold averment about this true existence of this statue).
When the statue was finished, it was dedicated with a poem:
To you, o Sun, the people of Dorian Rhodes set up this bronze statue reaching to Olympus, when they had pacified the waves of war and crowned their city with the spoils taken from the enemy. Not only over the seas but also on land did they kindle the lovely torch of freedom and independence. For to the descendants of Herakles belongs dominion over sea and land.
The Colossus stood gallantly at the harbour entrance for some fifty-six years. Each morning the sun must have caught its polished bronze surface and made the god’s figure shine. Then, unfortunately, an earthquake hit Rhodes in 226 BC and the statue collapsed. Huge pieces of the figure lay along the harbour for centuries. King Ptolemy III did offered a pay for its re-construction, however, the Rhodians refused because they say that it might because of God Helios’s indignation that he sent an earthquake and razed down the statue.
In the 7th Century, Arabians conquered Rhodes and took away all those statue debris and sell them in small pieces. Some says that it ‘employed’ 900 camels to carry all those remains!

domain: history


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Installation of Bluestack Step By Step With Images:
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- You can just go to the default location for installation.
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Installation of Nox Step By Step With Images.
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A simple, validated protocol consisting of a battery of tests is available to identify elderly patients with frailty syndrome. This syndrome of decreased reserve and resistance to stressors increases in incidence with increasing age. In the elderly, frailty may pursue a step-wise loss of function from non-frail to pre-frail to frail. We studied frailty in HIV-infected patients and found that ~20% are frail using the Fried phenotype using stringent criteria developed for the elderly1,2. In HIV infection the syndrome occurs at a younger age.
HIV patients were checked for 1) unintentional weight loss; 2) slowness as determined by walking speed; 3) weakness as measured by a grip dynamometer; 4) exhaustion by responses to a depression scale; and 5) low physical activity was determined by assessing kilocalories expended in a week's time. Pre-frailty was present with any two of five criteria and frailty was present if any three of the five criteria were abnormal.
The tests take approximately 10-15 min to complete and they can be performed by medical assistants during routine clinic visits. Test results are scored by referring to standard tables. Understanding which of the five components contribute to frailty in an individual patient can allow the clinician to address relevant underlying problems, many of which are not evident in routine HIV clinic visits.
20 Related JoVE Articles!
Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
Institutions: University of Duisburg-Essen.
The idea of collecting blood on a paper card and subsequently using the dried blood spots (DBS) for diagnostic purposes originated a century ago. Since then, DBS testing for decades has remained predominantly focused on the diagnosis of infectious diseases especially in resource-limited settings or the systematic screening of newborns for inherited metabolic disorders and only recently have a variety of new and innovative DBS applications begun to emerge. For many years, pre-analytical variables were only inappropriately considered in the field of DBS testing and even today, with the exception of newborn screening, the entire pre-analytical phase, which comprises the preparation and processing of DBS for their final analysis has not been standardized. Given this background, a comprehensive step-by-step protocol, which covers al the essential phases, is proposed, i.e.,
collection of blood; preparation of blood spots; drying of blood spots; storage and transportation of DBS; elution of DBS, and finally analyses of DBS eluates. The effectiveness of this protocol was first evaluated with 1,762 coupled serum/DBS pairs for detecting markers of hepatitis B virus, hepatitis C virus, and human immunodeficiency virus infections on an automated analytical platform. In a second step, the protocol was utilized during a pilot study, which was conducted on active drug users in the German cities of Berlin and Essen.
Molecular Biology, Issue 97, Dried blood spots, filter paper cards, specimen storage, infectious diseases, hepatitis B virus, hepatitis C virus, human immunodeficiency virus
Development of an IFN-γ ELISpot Assay to Assess Varicella-Zoster Virus-specific Cell-mediated Immunity Following Umbilical Cord Blood Transplantation
Institutions: Université de Montréal, Université de Montréal, Université de Montréal.
Varicella zoster virus (VZV) is a significant cause of morbidity and mortality following umbilical cord blood transplantation (UCBT). For this reason, antiherpetic prophylaxis is administrated systematically to pediatric UCBT recipients to prevent complications associated with VZV infection, but there is no strong, evidence based consensus that defines its optimal duration. Because T cell mediated immunity is responsible for the control of VZV infection, assessing the reconstitution of VZV specific T cell responses following UCBT could provide indications as to whether prophylaxis should be maintained or can be discontinued. To this end, a VZV specific gamma interferon (IFN-γ) enzyme-linked immunospot (ELISpot) assay was developed to characterize IFN-γ production by T lymphocytes in response to in vitro
stimulation with irradiated live attenuated VZV vaccine. This assay provides a rapid, reproducible and sensitive measurement of VZV specific cell mediated immunity suitable for monitoring the reconstitution of VZV specific immunity in a clinical setting and assessing immune responsiveness to VZV antigens.
Immunology, Issue 89, Varicella zoster virus, cell-mediated immunity, T cells, interferon gamma, ELISpot, umbilical cord blood transplantation
Writing and Low-Temperature Characterization of Oxide Nanostructures
Institutions: University of Pittsburgh.
Oxide nanoelectronics is a rapidly growing field which seeks to develop novel materials with multifunctional behavior at nanoscale dimensions. Oxide interfaces exhibit a wide range of properties that can be controlled include conduction, piezoelectric behavior, ferromagnetism, superconductivity and nonlinear optical properties. Recently, methods for controlling these properties at extreme nanoscale dimensions have been discovered and developed. Here are described explicit step-by-step procedures for creating LaAlO3
nanostructures using a reversible conductive atomic force microscopy technique. The processing steps for creating electrical contacts to the LaAlO3
interface are first described. Conductive nanostructures are created by applying voltages to a conductive atomic force microscope tip and locally switching the LaAlO3
interface to a conductive state. A versatile nanolithography toolkit has been developed expressly for the purpose of controlling the atomic force microscope (AFM) tip path and voltage. Then, these nanostructures are placed in a cryostat and transport measurements are performed. The procedures described here should be useful to others wishing to conduct research in oxide nanoelectronics.
Physics, Issue 89,
oxide nanostructures; nanoelectronics; semiconductor; LaAlO3/SrTiO3; lithography; AFM nanolithography
Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples
Institutions: University of Manitoba, University of Manitoba.
Despite the public health importance of mucosal pathogens (including HIV), relatively little is known about mucosal immunity, particularly at the female genital tract (FGT). Because heterosexual transmission now represents the dominant mechanism of HIV transmission, and given the continual spread of sexually transmitted infections (STIs), it is critical to understand the interplay between host and pathogen at the genital mucosa. The substantial gaps in knowledge around FGT immunity are partially due to the difficulty in successfully collecting and processing mucosal samples. In order to facilitate studies with sufficient sample size, collection techniques must be minimally invasive and efficient. To this end, a protocol for the collection of cervical cytobrush samples and subsequent isolation of cervical mononuclear cells (CMC) has been optimized. Using ex vivo
flow cytometry-based immunophenotyping, it is possible to accurately and reliably quantify CMC lymphocyte/monocyte population frequencies and phenotypes. This technique can be coupled with the collection of cervical-vaginal lavage (CVL), which contains soluble immune mediators including cytokines, chemokines and anti-proteases, all of which can be used to determine the anti- or pro-inflammatory environment in the vagina.
Medicine, Issue 89, mucosal, immunology, FGT, lavage, cervical, CMC
Radio Frequency Identification and Motion-sensitive Video Efficiently Automate Recording of Unrewarded Choice Behavior by Bumblebees
Institutions: University of Ottawa.
We present two methods for observing bumblebee choice behavior in an enclosed testing space. The first method consists of Radio Frequency Identification (RFID) readers built into artificial flowers that display various visual cues, and RFID tags (i.e.
, passive transponders) glued to the thorax of bumblebee workers. The novelty in our implementation is that RFID readers are built directly into artificial flowers that are capable of displaying several distinct visual properties such as color, pattern type, spatial frequency (i.e.
, “busyness” of the pattern), and symmetry (spatial frequency and symmetry were not manipulated in this experiment). Additionally, these visual displays in conjunction with the automated systems are capable of recording unrewarded
choice behavior. The second method consists of recording choice behavior at artificial flowers using motion-sensitive high-definition camcorders. Bumblebees have number tags glued to their thoraces for unique identification. The advantage in this implementation over RFID is that in addition to observing landing behavior, alternate measures of preference such as hovering and antennation may also be observed. Both automation methods increase experimental control, and internal validity by allowing larger scale studies that take into account individual differences. External validity is also improved because bees can freely enter and exit the testing environment without constraints such as the availability of a research assistant on-site. Compared to human observation in real time, the automated methods are more cost-effective and possibly less error-prone.
Neuroscience, Issue 93, bumblebee, unlearned behaviors, floral choice, visual perception, Bombus spp, information processing, radio-frequency identification, motion-sensitive video
Population Replacement Strategies for Controlling Vector Populations and the Use of Wolbachia pipientis for Genetic Drive
Institutions: Johns Hopkins University.
In this video, Jason Rasgon discusses population replacement strategies to control vector-borne diseases such as malaria and dengue. "Population replacement" is the replacement of wild vector populations (that are competent to transmit pathogens) with those that are not competent to transmit pathogens. There are several theoretical strategies to accomplish this. One is to exploit the maternally-inherited symbiotic bacteria Wolbachia pipientis. Wolbachia is a widespread reproductive parasite that spreads in a selfish manner at the extent of its host's fitness. Jason Rasgon discusses, in detail, the basic biology of this bacterial symbiont and various ways to use it for control of vector-borne diseases.
Cellular Biology, Issue 5, mosquito, malaria, genetics, infectious disease, Wolbachia
Fecal Microbiota Transplantation via Colonoscopy for Recurrent C. difficile Infection
Institutions: Brigham and Women‘s Hospital.
Fecal Microbiota Transplantation (FMT) is a safe and highly effective treatment for recurrent and refractory C. difficile
infection (CDI). Various methods of FMT administration have been reported in the literature including nasogastric tube, upper endoscopy, enema and colonoscopy. FMT via
colonoscopy yields excellent cure rates and is also well tolerated. We have found that patients find this an acceptable and tolerable mode of delivery. At our Center, we have initiated a fecal transplant program for patients with recurrent or refractory CDI. We have developed a protocol using an iterative process of revision and have performed 24 fecal transplants on 22 patients with success rates comparable to the current published literature. A systematic approach to patient and donor screening, preparation of stool, and delivery of the stool maximizes therapeutic success. Here we detail each step of the FMT protocol that can be carried out at any endoscopy center with a high degree of safety and success.
Immunology, Issue 94, C.difficile, colonoscopy, fecal transplant, stool, diarrhea, microbiota
Non-fluoroscopic Catheter Tracking for Fluoroscopy Reduction in Interventional Electrophysiology
Institutions: University of Leipzig.
A technological platform (MediGuide) has been recently introduced for non-fluoroscopic catheter tracking. In several studies, we have demonstrated that the application of this non-fluoroscopic catheter visualization system (NFCV) reduces fluoroscopy time and dose by 90-95% in a variety of electrophysiology (EP) procedures. This can be of relevance not only to the patients, but also to the nurses and physicians working in the EP lab. Furthermore, in a subset of indications such as supraventricular tachycardias, NFCV enables a fully non-fluoroscopic procedure and allows the lab staff to work without wearing lead aprons. With this protocol, we demonstrate that even complex procedures such as ablations of atrial fibrillation, that are typically associated with fluoroscopy times of >30 min in conventional settings, can safely be performed with a reduction of >90% in fluoroscopy exposure by the additional use of NFCV.
Medicine, Issue 99, Fluoroscopy, ablation, radiation exposure, atrial fibrillation, 3D mapping, electrophysiology
Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
Institutions: United States Department of Agriculture, Iowa State University, UK Veterinary Laboratories Agency, United States Department of Agriculture.
Effector and memory T cells are generated through developmental programing of naïve cells following antigen recognition. If the infection is controlled up to 95 % of the T cells generated during the expansion phase are eliminated (i.e
., contraction phase) and memory T cells remain, sometimes for a lifetime. In humans, two functionally distinct subsets of memory T cells have been described based on the expression of lymph node homing receptors. Central memory T cells express C-C chemokine receptor 7 and CD45RO and are mainly located in T-cell areas of secondary lymphoid organs. Effector memory T cells express CD45RO, lack CCR7 and display receptors associated with lymphocyte homing to peripheral or inflamed tissues. Effector T cells do not express either CCR7 or CD45RO but upon encounter with antigen produce effector cytokines, such as interferon-γ. Interferon-γ release assays are used for the diagnosis of bovine and human tuberculosis and detect primarily effector and effector memory T cell responses. Central memory T cell responses by CD4+
T cells to vaccination, on the other hand, may be used to predict vaccine efficacy, as demonstrated with simian immunodeficiency virus infection of non-human primates, tuberculosis in mice, and malaria in humans. Several studies with mice and humans as well as unpublished data on cattle, have demonstrated that interferon-γ ELISPOT assays measure central memory T cell responses. With this assay, peripheral blood mononuclear cells are cultured in decreasing concentration of antigen for 10 to 14 days (long-term culture), allowing effector responses to peak and wane; facilitating central memory T cells to differentiate and expand within the culture.
Immunology, Issue 101, Immunology, bovine tuberculosis, CD4 T cells, vaccine
The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo
Institutions: Australian National University.
The ability to monitor T cell responses in vivo
is important for the development of our understanding of the immune response and the design of immunotherapies. Here we describe the use of fluorescent target array (FTA) technology, which utilizes vital dyes such as carboxyfluorescein succinimidyl ester (CFSE), violet laser excitable dyes (CellTrace Violet: CTV) and red laser excitable dyes (Cell Proliferation Dye eFluor 670: CPD) to combinatorially label mouse lymphocytes into >250 discernable fluorescent cell clusters. Cell clusters within these FTAs can be pulsed with major histocompatibility (MHC) class-I and MHC class-II binding peptides and thereby act as target cells for CD8+
T cells, respectively. These FTA cells remain viable and fully functional, and can therefore be administered into mice to allow assessment of CD8+
T cell-mediated killing of FTA target cells and CD4+
T cell-meditated help of FTA B cell target cells in real time in vivo
by flow cytometry. Since >250 target cells can be assessed at once, the technique allows the monitoring of T cell responses against several antigen epitopes at several concentrations and in multiple replicates. As such, the technique can measure T cell responses at both a quantitative (e.g.
the cumulative magnitude of the response) and a qualitative (e.g
. functional avidity and epitope-cross reactivity of the response) level. Herein, we describe how these FTAs are constructed and give an example of how they can be applied to assess T cell responses induced by a recombinant pox virus vaccine.
Immunology, Issue 88, Investigative Techniques, T cell response, Flow Cytometry, Multiparameter, CTL assay in vivo, carboxyfluorescein succinimidyl ester (CFSE), CellTrace Violet (CTV), Cell Proliferation Dye eFluor 670 (CPD)
A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses
Institutions: Emory University, Emory University.
The protective effect of many HLA class I alleles on HIV-1 pathogenesis and disease progression is, in part, attributed to their ability to target conserved portions of the HIV-1 genome that escape with difficulty. Sequence changes attributed to cellular immune pressure arise across the genome during infection, and if found within conserved regions of the genome such as Gag, can affect the ability of the virus to replicate in vitro
. Transmission of HLA-linked polymorphisms in Gag to HLA-mismatched recipients has been associated with reduced set point viral loads. We hypothesized this may be due to a reduced replication capacity of the virus. Here we present a novel method for assessing the in vitro
replication of HIV-1 as influenced by the gag
gene isolated from acute time points from subtype C infected Zambians. This method uses restriction enzyme based cloning to insert the gag
gene into a common subtype C HIV-1 proviral backbone, MJ4. This makes it more appropriate to the study of subtype C sequences than previous recombination based methods that have assessed the in vitro
replication of chronically derived gag-pro
sequences. Nevertheless, the protocol could be readily modified for studies of viruses from other subtypes. Moreover, this protocol details a robust and reproducible method for assessing the replication capacity of the Gag-MJ4 chimeric viruses on a CEM-based T cell line. This method was utilized for the study of Gag-MJ4 chimeric viruses derived from 149 subtype C acutely infected Zambians, and has allowed for the identification of residues in Gag that affect replication. More importantly, the implementation of this technique has facilitated a deeper understanding of how viral replication defines parameters of early HIV-1 pathogenesis such as set point viral load and longitudinal CD4+ T cell decline.
Infectious Diseases, Issue 90, HIV-1, Gag, viral replication, replication capacity, viral fitness, MJ4, CEM, GXR25
Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model
Institutions: National Health Laboratory Services (NHLS-SA), University of Witwatersrand, Lightcurve Films.
We present the video about assisting anti-retroviral therapy (ART) by an apt laboratory service - representing a South-African role model for economical large scale diagnostic testing. In the low-income countries inexpensive ART has transformed the prospects for the survival of HIV seropositive patients but there are doubts whether there is a need for the laboratory monitoring of ART and at what costs - in situations when the overall quality of pathology services can still be very low. The appropriate answer is to establish economically sound services with better coordination and stricter internal quality assessment than seen in western countries. This video, photographed at location in the National Health Laboratory Services (NHLS-SA) at the Witwatersrand University, Johannesburg, South Africa, provides such a coordinated scheme expanding the original 2-color CD4-CD45 PanLeucoGating strategy (PLG). Thus the six modules of the video presentation reveal the simplicity of a 4-color flow cytometric assay to combine haematological, immunological and virology-related tests in a single tube. These video modules are: (i) the set-up of instruments; (ii) sample preparations; (iii) testing absolute counts and monitoring quality for each sample by bead-count-rate; (iv) the heamatological CD45 test for white cell counts and differentials; (v) the CD4 counts, and (vi) the activation of CD8+ T cells measured by CD38 display, a viral load related parameter. The potential cost-savings are remarkable. This arrangement is a prime example for the feasibility of performing > 800-1000 tests per day with a stricter quality control than that applied in western laboratories, and also with a transfer of technology to other laboratories within a NHLS-SA network. Expert advisors, laboratory managers and policy makers who carry the duty of making decisions about introducing modern medical technology are frequently not in a position to see the latest technical details as carried out in the large regional laboratories with huge burdens of workload. Hence this video shows details of these new developments.
Immunology, Issue 44, Human Immunodeficiency virus (HIV); CD4 lymphocyte count, white cell count, CD45, panleucogating, lymphocyte activation, CD38, HIV viral load, antiretroviral therapy (ART), internal quality control
Determining Soil-transmitted Helminth Infection Status and Physical Fitness of School-aged Children
Institutions: Swiss Tropical and Public Health Institute, Basel, Switzerland, University of Basel, Basel, Switzerland.
Soil-transmitted helminth (STH) infections are common. Indeed, more than 1 billion people are affected, mainly in the developing world where poverty prevails and hygiene behavior, water supply, and sanitation are often deficient1,2
. Ascaris lumbricoides
, Trichuris trichiura
, and the two hookworm species, Ancylostoma duodenale
and Necator americanus
, are the most prevalent STHs3
. The estimated global burden due to hookworm disease, ascariasis, and trichuriasis is 22.1, 10.5, and 6.4 million disability-adjusted life years (DALYs), respectively4
. Furthermore, an estimated 30-100 million people are infected with Strongyloides stercoralis
, the most neglected STH species of global significance which arguably also causes a considerable public health impact5,6
. Multiple-species infections (i.e., different STHs harbored in a single individual) are common, and infections have been linked to lowered productivity and thus economic outlook of developing countries1,3
For the diagnosis of common STHs, the World Health Organization (WHO) recommends the Kato-Katz technique7,8
, which is a relatively straightforward method for determining the prevalence and intensity of such infections. It facilitates the detection of parasite eggs that infected subjects pass in their feces.
With regard to the diagnosis of S.stercoralis
, there is currently no simple and accurate tool available. The Baermann technique is the most widely employed method for its diagnosis. The principle behind the Baermann technique is that active S.stercoralis
larvae migrate out of an illuminated fresh fecal sample as the larvae are phototactic9
. It requires less sophisticated laboratory materials and is less time consuming than culture and immunological methods5
Morbidities associated with STH infections range from acute but common symptoms, such as abdominal pain, diarrhea, and pruritus, to chronic symptoms, such as anemia, under- and malnutrition, and cognitive impairment10
. Since the symptoms are generally unspecific and subtle, they often go unnoticed, are considered a normal condition by affected individuals, or are treated as symptoms of other diseases that might be more common in a given setting. Hence, it is conceivable that the true burden of STH infections is underestimated by assessment tools relying on self-declared signs and symptoms as is usually the case in population-based surveys.
In the late 1980s and early 1990s, Stephenson and colleagues highlighted the possibility of STH infections lowering the physical fitness of boys aged 6-12 years11,12
. This line of scientific inquiry gained new momentum recently13,14,15
. The 20-meter (m) shuttle run test was developed and validated by Léger et al.16
and is used worldwide to measure the aerobic fitness of children17
. The test is easy to standardize and can be performed wherever a 20-m long and flat running course and an audio source are available, making its use attractive in resource-constrained settings13
. To facilitate and standardize attempts at assessing whether STH infections have an effect on the physical fitness of school-aged children, we present methodologies that diagnose STH infections or measure physical fitness that are simple to execute and yet, provide accurate and reproducible outcomes. This will help to generate new evidence regarding the health impact of STH infections.
Infection, Issue 66, Immunology, Medicine, Infectious Diseases, Soil-transmitted helminths, physical fitness, Kato-Katz technique, Baermann technique, 20-meter shuttle run test, children
Isolation of Lymphocytes from Mouse Genital Tract Mucosa
Institutions: University of California, Los Angeles , California NanoSystems.
Mucosal surfaces, including in the gastrointestinal, urogenital, and respiratory tracts, provide portals of entry for pathogens, such as viruses and bacteria 1
. Mucosae are also inductive sites in the host to generate immunity against pathogens, such as the Peyers patches in the intestinal tract and the nasal-associated lymphoreticular tissue in the respiratory tract. This unique feature brings mucosal immunity as a crucial player of the host defense system. Many studies have been focused on gastrointestinal and respiratory mucosal sites. However, there has been little investigation of reproductive mucosal sites. The genital tract mucosa is the primary infection site for sexually transmitted diseases (STD), including bacterial and viral infections. STDs are one of the most critical health challenges facing the world today. Centers for Disease Control and Prevention estimates that there are 19 million new infectious every year in the United States. STDs cost the U.S. health care system $17 billion every year 2
, and cost individuals even more in immediate and life-long health consequences. In order to confront this challenge, a greater understanding of reproductive mucosal immunity is needed and isolating lymphocytes is an essential component of these studies. Here, we present a method to reproducibly isolate lymphocytes from murine female genital tracts for immunological studies that can be modified for adaption to other species. The method described below is based on one mouse.
Immunology, Issue 67, Mucosal immunity, sexually transmitted diseases, genital tract lymphocytes, lymphocyte isolation, flow cytometry, FACS
New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals
Institutions: Ragon Institute of MGH, MIT, and Harvard, Massachusetts General Hospital.
CD4+ Regulatory T cells (Tregs) are potent immune modulators and serve an important function in human immune homeostasis. Depletion of Tregs has led to measurable increases in antigen-specific T cell responses in vaccine settings for cancer and infectious pathogens. However, their role in HIV-1 immuno-pathogenesis remains controversial, as they could either serve to suppress deleterious HIV-1-associated immune activation and thus slow HIV-1 disease progression or alternatively suppress HIV-1-specific immunity and thereby promote virus spread. Understanding and modulating Treg function in the context of HIV-1 could lead to potential new strategies for immunotherapy or HIV vaccines. However, important open questions remain on their role in the context of HIV-1 infection, which needs to be carefully studied.
Representing roughly 5% of human CD4+ T cells in the peripheral blood, studying the Treg population has proven to be difficult, especially in HIV-1 infected individuals where HIV-1-associated CD4 T cell and with that Treg depletion occurs. The characterization of regulatory T cells in individuals with advanced HIV-1 disease or tissue samples, for which only very small biological samples can be obtained, is therefore extremely challenging. We propose a technical solution to overcome these limitations using isolation and expansion of Tregs from HIV-1-positive individuals.
Here we describe an easy and robust method to successfully expand Tregs isolated from HIV-1-infected individuals in vitro
. Flow-sorted CD3+
Tregs were stimulated with anti-CD3/anti-CD28 coated beads and cultured in the presence of IL-2. The expanded Tregs expressed high levels of FOXP3, CTLA4 and HELIOS compared to conventional T cells and were shown to be highly suppressive. Easier access to large numbers of Tregs will allow researchers to address important questions concerning their role in HIV-1 immunopathogenesis. We believe answering these questions may provide useful insight for the development of an effective HIV-1 vaccine.
Infection, Issue 75, Infectious Diseases, Medicine, Immunology, Virology, Cellular Biology, Molecular Biology, Lymphocytes, T-Lymphocytes, Regulatory, HIV, Culture Techniques, flow cytometry, cell culture, Treg expansion, regulatory T cells, CD4+ T cells, Tregs, HIV-1, virus, HIV-1 infection, AIDS, clinical techniques
An Experimental Model to Study Tuberculosis-Malaria Coinfection upon Natural Transmission of Mycobacterium tuberculosis and Plasmodium berghei
Institutions: University Hospital Heidelberg, Research Center Borstel.
Coinfections naturally occur due to the geographic overlap of distinct types of pathogenic organisms. Concurrent infections most likely modulate the respective immune response to each single pathogen and may thereby affect pathogenesis and disease outcome. Coinfected patients may also respond differentially to anti-infective interventions. Coinfection between tuberculosis as caused by mycobacteria and the malaria parasite Plasmodium
, both of which are coendemic in many parts of sub-Saharan Africa, has not been studied in detail. In order to approach the challenging but scientifically and clinically highly relevant question how malaria-tuberculosis coinfection modulate host immunity and the course of each disease, we established an experimental mouse model that allows us to dissect the elicited immune responses to both pathogens in the coinfected host. Of note, in order to most precisely mimic naturally acquired human infections, we perform experimental infections of mice with both pathogens by their natural routes of infection, i.e.
aerosol and mosquito bite, respectively.
Infectious Diseases, Issue 84, coinfection, mouse, Tuberculosis, Malaria, Plasmodium berghei, Mycobacterium tuberculosis, natural transmission
Rescue of Recombinant Newcastle Disease Virus from cDNA
Institutions: Icahn School of Medicine at Mount Sinai, Icahn School of Medicine at Mount Sinai, Icahn School of Medicine at Mount Sinai, University of Rochester.
Newcastle disease virus (NDV), the prototype member of the Avulavirus
genus of the family Paramyxoviridae1
, is a non-segmented, negative-sense, single-stranded, enveloped RNA virus (Figure 1)
with potential applications as a vector for vaccination and treatment of human diseases. In-depth exploration of these applications has only become possible after the establishment of reverse genetics techniques to rescue recombinant viruses from plasmids encoding their complete genomes as cDNA2-5
. Viral cDNA can be conveniently modified in vitro
by using standard cloning procedures to alter the genotype of the virus and/or to include new transcriptional units. Rescue of such genetically modified viruses provides a valuable tool to understand factors affecting multiple stages of infection, as well as allows for the development and improvement of vectors for the expression and delivery of antigens for vaccination and therapy. Here we describe a protocol for the rescue of recombinant NDVs.
Immunology, Issue 80, Paramyxoviridae, Vaccines, Oncolytic Virotherapy, Immunity, Innate, Newcastle disease virus (NDV), MVA-T7, reverse genetics techniques, plasmid transfection, recombinant virus, HA assay
Intralymphatic Immunotherapy and Vaccination in Mice
Institutions: University Hospital Zurich.
Vaccines are typically injected subcutaneously or intramuscularly for stimulation of immune responses. The success of this requires efficient drainage of vaccine to lymph nodes where antigen presenting cells can interact with lymphocytes for generation of the wanted immune responses. The strength and the type of immune responses induced also depend on the density or frequency of interactions as well as the microenvironment, especially the content of cytokines. As only a minute fraction of peripherally injected vaccines reaches the lymph nodes, vaccinations of mice and humans were performed by direct injection of vaccine into inguinal lymph nodes, i.e.
intralymphatic injection. In man, the procedure is guided by ultrasound. In mice, a small (5-10 mm) incision is made in the inguinal region of anesthetized animals, the lymph node is localized and immobilized with forceps, and a volume of 10-20 μl of the vaccine is injected under visual control. The incision is closed with a single stitch using surgical sutures. Mice were vaccinated with plasmid DNA, RNA, peptide, protein, particles, and bacteria as well as adjuvants, and strong improvement of immune responses against all type of vaccines was observed. The intralymphatic method of vaccination is especially appropriate in situations where conventional vaccination produces insufficient immunity or where the amount of available vaccine is limited.
Immunology, Issue 84, Vaccination, Immunization, intralymphatic immunotherapy, Lymph node injection, vaccines, adjuvants, surgery, anesthesia
A Protocol for Analyzing Hepatitis C Virus Replication
Institutions: Cedars-Sinai Medical Center, David Geffen School of Medicine at UCLA.
Hepatitis C Virus (HCV) affects 3% of the world’s population and causes serious liver ailments including chronic hepatitis, cirrhosis, and hepatocellular carcinoma. HCV is an enveloped RNA virus belonging to the family Flaviviridae
. Current treatment is not fully effective and causes adverse side effects. There is no HCV vaccine available. Thus, continued effort is required for developing a vaccine and better therapy. An HCV cell culture system is critical for studying various stages of HCV growth including viral entry, genome replication, packaging, and egress. In the current procedure presented, we used a wild-type intragenotype 2a chimeric virus, FNX-HCV, and a recombinant FNX-Rluc virus carrying a Renilla
luciferase reporter gene to study the virus replication. A human hepatoma cell line (Huh-7 based) was used for transfection of in vitro
transcribed HCV genomic RNAs. Cell-free culture supernatants, protein lysates and total RNA were harvested at various time points post-transfection to assess HCV growth. HCV genome replication status was evaluated by quantitative RT-PCR and visualizing the presence of HCV double-stranded RNA. The HCV protein expression was verified by Western blot and immunofluorescence assays using antibodies specific for HCV NS3 and NS5A proteins. HCV RNA transfected cells released infectious particles into culture supernatant and the viral titer was measured. Luciferase assays were utilized to assess the replication level and infectivity of reporter HCV. In conclusion, we present various virological assays for characterizing different stages of the HCV replication cycle.
Infectious Diseases, Issue 88, Hepatitis C Virus, HCV, Tumor-virus, Hepatitis C, Cirrhosis, Liver Cancer, Hepatocellular Carcinoma
Establishment and Characterization of UTI and CAUTI in a Mouse Model
Institutions: Washington University School of Medicine.
Urinary tract infections (UTI) are highly prevalent, a significant cause of morbidity and are increasingly resistant to treatment with antibiotics. Females are disproportionately afflicted by UTI: 50% of all women will have a UTI in their lifetime. Additionally, 20-40% of these women who have an initial UTI will suffer a recurrence with some suffering frequent recurrences with serious deterioration in the quality of life, pain and discomfort, disruption of daily activities, increased healthcare costs, and few treatment options other than long-term antibiotic prophylaxis. Uropathogenic Escherichia coli
(UPEC) is the primary causative agent of community acquired UTI. Catheter-associated UTI (CAUTI) is the most common hospital acquired infection accounting for a million occurrences in the US annually and dramatic healthcare costs. While UPEC is also the primary cause of CAUTI, other causative agents are of increased significance including Enterococcus faecalis
. Here we utilize two well-established mouse models that recapitulate many of the clinical characteristics of these human diseases. For UTI, a C3H/HeN model recapitulates many of the features of UPEC virulence observed in humans including host responses, IBC formation and filamentation. For CAUTI, a model using C57BL/6 mice, which retain catheter bladder implants, has been shown to be susceptible to E. faecalis
bladder infection. These representative models are being used to gain striking new insights into the pathogenesis of UTI disease, which is leading to the development of novel therapeutics and management or prevention strategies.
Medicine, Issue 100, Escherichia coli, UPEC, Enterococcus faecalis, uropathogenic, catheter, urinary tract infection, IBC, chronic cystitis


text:
DEARBORN – A federal judge has dismissed a lawsuit against the city of Dearborn and a city of Dearborn police officer in the shooting death of a man in the police station lobby on Dec. 18, 2022.
On that date, 33-year-old Ali Naji entered the Dearborn police station holding a firearm and attempted to shoot at an officer in the lobby. His gun malfunctioned and during that time, the police officer behind the front desk fatally shot him.
The shooting at that time caused controversy in the community and Naji’s family filed a wrongful death lawsuit against Dearborn police. Both the Dearborn Police and the Michigan State Police carried out separate investigations into the incident.
U.S. District Judge F. Kay Behm noted in her decision that the involved police officer witnessed a clear threat to himself and others when a man entered the lobby of the Dearborn Police Department and, without warning, pointed a gun at the officer, pulled the trigger and then attempted to correct his malfunctioning gun.
The judge unequivocally determined that the officer’s use of deadly force against the individual “was not excessive” and that had he not engaged the armed individual, he “would have left an armed man who appeared willing to use his firearm alone in the police station lobby where members of the public, other officers and staff could enter at any moment.”
Dearborn Police Chief Issa Shahin expressed gratitude for the court’s decision and stated the officer faced a significant threat to himself, fellow officers and the public. Despite welcoming the decision, Chief Shahin expressed condolences to the deceased’s family and the officers present during the incident.
“While we welcome the Court’s decision, our thoughts remain with the family of the deceased and the officers that were present that day,” Shahin said.

domain: news


text:
Greta Van Susteren will launch a new political talk program Sunday night, with her first guests including presidential candidate Rep. Tulsi GabbardTulsi GabbardThe perfect Democratic running mate for DeSantis? Progressives breathe sigh of relief after Afghan withdrawal Hillicon Valley: US has made progress on cyber but more needed, report says | Democrat urges changes for 'problematic' crypto language in infrastructure bill | Facebook may be forced to unwind Giphy acquisition MORE (D-Hawaii) and former NFL coach Tony Dungy.
"Full Court Press" will be on in 93 markets including New York, Washington and Los Angeles via Atlanta-based Gray Television.
Van Susteren has hosted programs on CNN, Fox News and MSNBC going back to 1994. Her most recent employer was Voice of America, where she hosted a weekly 30-minute program called “Plugged In With Greta Van Susteren.”
"When I was on cable, I had about five or six bureaus. Now that I'm with Gray, they have 143 stations in 93 markets. Gray has all these stations in important states for the 2020 elections. I have correspondents virtually peppering the entire country, so I have incredible access," Van Susteren said in a phone interview with The Hill.
“Then I dug a little deeper, and I guess I discovered the obvious: The American viewer trusts local news far more than it trusts national or cable. And I thought: Maybe that trust can rub off on me. So I thought this might be exactly what I'm looking for," the former attorney added.
Van Susteren will provide Gray TV's newsrooms "with the expert, unbiased coverage of national and international political developments that have been the hallmark of her distinguished news career," the company said. "In addition, she has two nationally syndicated shows in development with Gray."
“The show will tap the knowledge and expertise of Gray Television’s trusted bench of reporters who will provide on-the-ground reports from their communities,” said Sandy Breland, senior vice president of Gray TV. "It’s a perfect time to highlight politics through the local lens as viewers rank local news as their most trusted source. We look forward to Greta bringing her firm but fair approach to broadcast television.”
Van Susteren says that she specifically wanted Gabbard on her program because of how she stands out in the wide 2020 Democratic primary field.
"The reason why I wanted Tulsi Gabbard is because out of the pack of 20 or so Democrats that are running, she's probably the one that is most unlike the others. So I wanted to hear from her," she says. "Because the other ones have policies that pretty much match each other, and it becomes a beauty contest among the others because they have so much in common."
The program will air out of Washington, with starting times varying station to station on Sundays.


text:
COLUMBUS, Ohio — The Ohio Farm Bureau Federation members will be “Together Again” as the organization holds its 103rd annual meeting Dec. 9 and 10 in Columbus.
Nearly 385 delegates from all Ohio counties will establish the organization’s policy goals for the coming year, elect leaders, recognize county Farm Bureau and individual achievements and honor industry leaders.
Keynote speeches will be delivered by Ohio Farm Bureau President Bill Patterson and the organization’s Executive Vice President Adam Sharp. Attendees also will hear from Nationwide Chief Executive Officer Kirt Walker.
County Farm Bureaus will be recognized for innovative local programming, and individuals will be honored for their work to build membership and their contributions to the farm and food community.
Ohio Farm Bureau’s Young Agricultural Professionals State Committee will name their new leadership and the 2021 YAP award winners will be recognized.
The inaugural Ohio Farm Bureau Foundation Breakfast takes place Dec. 10 in conjunction with the annual meeting. The event will highlight the foundation’s commitment to supporting the future of Ohio agriculture, and the award of the “Y Prize,” honoring farmer mental health initiatives, will be presented.
Get a full schedule of the 2021 Ohio Farm Bureau Annual Meeting and watch some of the events live at ohiofarmbureau.org/annualmeeting.
Ohio Farm Bureau’s mission is working together with Ohio farmers to advance agriculture and strengthen the state’s communities. Learn more at ohiofarmbureau.org.
— Ohio Farm Bureau Federation

domain: agronomy