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It is known fact that the majority of the 3.000 spiders in the United States of America are not poisonous . Also it is known that the most spiders bite but they have fangs which are too small or weak to puncture our skin. You should know that the spider bite looks like any other bug bite – an inflamed, red, sometimes painful or itchy bump on our skin – and it can go unnoticed. Harmless spider bites are not producing any other symptoms. The spiders that do manage to bite through your skin and insert toxic venom, can cause serious complications for our health. It is very easy to identify a spider bite if you saw that the spider has bitten you, but also there are some cases when you will not notice the wound until hours have passed. You should look for things such as skin damage, a red welt, swelling and any troubling symptoms that will accompany the bite. Also there are other possible symptoms which can accompany the spider bite such as
- High blood pressure
- Swollen lymph glands
- Anxiety or restlessness
- Nausea and vomiting
- Difficulty breathing
- Blister that is red or purple in color
- Muscle pain or cramping
- Pain around the area of the bite
- Itching or rash
There are many cases when spider bites are taking longer period to heal compared to other insect bites. Also these types of bites can affect skin tissues. It is very important to keep the bite clean because this will help you to reduce the risk of infection.
Black widow spider bites
Here are signs and symptoms of black widow spider bite :
- Sweating: When this type of spider bites you, then excessive sweating can happen.
- Pain: Typically pain is beginning with an hour of being bitten and the pain is generally happening around the bite mark but also it can spread from the bite side into your chest, back or abdomen.
- Cramping: Abdominal rigidity or cramping can be so severe and this in some cases is mistaken for a ruptured appendix or appendicitis.
The prompt treatment is the best, especially for older adults and children. There are some cases when healthcare professionals will prescribe antivenin to remove the venom from the human body.
Black widow habitat: This type of spider can be found throughout the United States of America but more so in the southwestern states. Black widows prefer to live in:
- Unused pots and gardening equipment
Hobo spider bites: This is a common spider in Pacific Northwest. These spiders sit up high on long legs and they run fast. When this spider bites you, then it can be unnoticeable first, but it will cause numbness and pain within fifteen minutes. After 60 minutes, the site will start to turn red. In 8 hours, it will become swollen and hardened. After 24 – 26 hours, the wound can discharge fluids and it will eventually turn black.
Brown recluse spider bites
The pain that is associated with this type of spider bite typically increases during the first 8 hours after the bite. You can also have body aches, chills and fever. This bite usually will heal on its own in a period of one week. There a minor number of cases, when the skin at the center of the bite can become dark purple or blue and it will evolve into a deep open sore (ulcer) which enlarges as the surrounding skin die. This ulcer will stop growing within ten days after the bite but the full healing can take months. There are some rare cases when the brown recluse bite can cause :
- Kidney failure
- Blood in urine
- Coma or seizures
There is no antidote for the bite of brown recluse spider but it is very important its treatment because when you keep the area clean, then it will encourage faster healing.
Brown recluse habitat: These types of spiders are most commonly found in the southern Midwest and in limited areas of the South. These types of spiders have this name because they like to hide away in undisturbed areas. Brown recluse mostly prefer to live indoors, in places such as
- In rarely used cupboards
- Behind bookshelves and dressers
- The clutter of basement or attics
When they are outside, then they seek out dark and quit spots such as in tree stumps or under rocks. There are very rare cases when the bite from a black widow or brown recluse can be deadly, particularly it is happening in children. [2,6]
 Louisiana Office of Public Health – Infectious Disease Epidemiology Section. Spider bites. 2007. Retrieved from ldh.la.gov/assets/oph/Center-PHCH/Center-CH/infectious-epi/EpiManual/SpiderBitesManual.pdf
 Rahmani F, Khojasteh SMB, Bakhtavar HE, et al. Poisonous spiders: Bites, symptoms, and treatment; an educational review. Emergency. 2014 Spring;2(2):54–8.
 Sulaj Z, Vyshka G, Gashi A. Analysis of cases caused by acute spider bite. Journal of Acute Disease. 2015;4(3):255-8.
 Anoka IA, Robb EL, Baker MB. Brown recluse spider toxicity. In Treasure Island (FL): StatPearls Publishing. 2020.
 Vanderbilt University Medical Center. Children at greater risk for complications from brown recluse spider bites. Science Daily. 2017. Retrieved from sciencedaily.com/releases/2017/04/170420090218.htm>.

domain: biology


text:
Adverse effects in the therapeutic use of androgens and anabolic congeners
Semantic Scholar uses AI to extract papers important to this topic.
In this paper, we review recent data for brominated flame retardants (particularly BDEs, HBCD and TBBP-A) in samples from the… (More)
For the general population the intake of food of animal origin is the main route of human exposure to polychlorinated dibenzo-p… (More)
- Alcoholism, clinical and experimental research
The ingestion of both ethanol and whisky can induce acute gastrointestinal bleeding. The effects of the congeners… (More)
A previously described method for analysis of organochlorine compounds in human milk was adopted for analysis of brominated… (More)
Aluminas, silicas and aluminosilicates were evaluated for their ability to sorb radiolabeled aflatoxin B1 (AFB1) from aqueous… (More)

domain: chemistry


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“Parallel worlds of cruelty in I’m Not Scared“, Dr Jennifer Minter (English Works Notes, 2017)
In I’m not Scared, Niccolo Ammaniti explores the corruption of the adult world through the eyes of the nine-year old narrator, Michele Amitrano, who chances upon a kidnapped child left to rot in a dilapidated house. The story charts Michele’s descent into the adult world of cruelty, greed and corruption and his subsequent dilemma.
Throughout the novel, Ammaniti parallels the childhood and the adult worlds to focus on the nature of evil and the possibility of goodness. Neither worlds, he suggests, are innocent. In fact, challenging stereotypes of children as innocent and naïve, he shows their equal capacity for deceit and cruelty. From the initial childish games of Salvatore Scardaccione’s (Skull’s) childhood gang to which Michele belongs, to the cruelty of the adult world, Ammaniti suggests that we all have a capacity to do irreparable harm to each other. Ammaniti suggests that when both worlds forfeit an intuitive capacity for moral kindness and compassion, all members of the group suffer. Without a moral beacon, and without the barriers of conscience, people become capable of untold violence and cruelty against each other. In such a world, a father is capable of killing his son – perhaps with or without his knowledge.
As a nine-year-old child, Michele champions all that is idyllic about childhood; the love and the innocence, the friendship and the fun. He cares for his sister and is protective and spontaneously sympathetic towards Filippo. In fact, it is his strong sense of duty and loyalty that embroils him in a terrible dilemma.
Michele’s carefree world is shattered when he becomes involved in a deadly game of “dare”. Ammaniti depicts Michele as one who is prepared to confront Skull’s authority and risk exclusion. This foreshadows his eventual challenge to his father and predicts his courage. Michele is not one to readily submit to the unjust authority of others.
As the leader of the children’s gang, Skull insists on unquestioned obedience and sees himself as the undisputed leader. He is cruel and viciously humiliates Barbara because of a sense of power over others. He is sadistic, thuggish, violent and threatens others through the use of violence and exclusion. He “revels” in her embarrassment when he makes her show her “tits” then her “slit”. He tells her to “pull down your trousers. That’ll teach you to fuck me around.”(20) Previously Barbara embarrassed Skull and made him look foolish, so he deliberately victimizes her in front of the gang members. Barbara tries to be resilient, and clings to her pride and dignity. She refuses to cry when he slaps her face for not getting his own way.
Michele stands up for Barbara and “plays the hero” (22) just as he will defend Filippo’s rights in the end. Ammaniti suggests that if someone does not protect the humanity of others, and their right to dignity and freedom, then the world will degenerate into savagery. Gaining the “forfeit”, Michele has to scramble up to the first floor of the house, jump out of the window and climb down the tree (22). Idyllic and simple, Michele’s childhood is disrupted by the discovery of the emaciated boy who appears dead.
One of Michele’s biggest fears arises from his encounter with death and decay. His fears begin to manifest when he stumbles across the apparently dead body of a child during their childhood adventure. “I must pluck up the courage and look”. Even though the distorted image created by his imagination gripped his “testicles with an icy hand”, Michele’s courage continuously brings him back to the body.
The discovery of the “dead” body in the large hole becomes a trigger for Michele’s fears that define him in increasingly moralistic and repulsive ways. The discovery of the boy’s body awakens his conscience because he instinctively feels that it is shrouded in injustice. The fact that the hole is a “couple of metres wide” and “two and a half metres” deep is enough to trigger the feeling that something is sinister, as symbolised by the darkness and the secrecy. Seeing the body move, Michele becomes increasingly anxious. He thinks he sees a “slight movement of the arm”.
Not only is this Michele’s first encounter with a dead body – apart from his grandmother’s – but his conscience is awakened. This is evident through the biblical imagery. In his nightmare, he recalls that Jesus told Lazarus “to rise and walk, but Lazarus didn’t rise”. Instead, Lazarus was defiant because he “wouldn’t come back to life”. Evidently, Michele is traumatized by the body that moved and he is unable to ascertain whether he is dead or alive, and what he should think or feel. The command, “leave the dead alone” leaves him covered in sweat. His fears soon takeover, because he is unable to follow a dictate that undermines his conscience.
Over time, Michele befriends and nurtures the boy. Ironically, despite his fears he tries to show that he is not scared. He tries to show the strength and conviction that lead to the discovery in the first place, when he defies Skull in order to protect Barbara from humiliation.
Michele’s fears emerge in the form of nightmares. Trolls, witches, “hunchbacked and wrinkled” bogeymen and fairytale monsters plague both his imagination and disturb his sense of reality. Stalking him in his sleep and on his trips to the mountain, he feels paranoid. Nevertheless, he defies his fears of monsters when his courage and imagination take him to the role-playing of his comic book hero, ‘Tiger Jack’. Michele quickly learns of the corruption in the adult world, and that “it’s men you should be afraid of, not monsters”.
Although we all experience fears and difficulties growing up, Ammaniti suggests that often our fears or the way we cope with our fears define us as people. The way we respond to our fears will have lasting consequences. Some withdraw, some become anxious and some become constantly fearful. For those of us who can stand their ground, confront their demons and learn to conquer their fear, there is a chance to develop strength and resilience.
However, what if the demons are simply overwhelming? What if there is no easy resolution or no easy course of action? It is a mark of Michele’s courage and strength that he does seek to confront his fears once he knows that the father is the “bogeyman”.
Michele’s childhood world transforms from a fairytale to a nightmare as the troubling signs of Papa’s complicity become evident. Michele suspects that the saucepan and apple belonged to Papa. Filippo’s exercise book is found in the old man’s suitcase. In this regard, the author subverts the purpose of the fairytale as a cautionary tale – in this story there is no happy ending. IN this case, there is no happy ending and the monsters are not so easily defeated.
Michele’s fears are exacerbated by the knowledge of corruption in the adult world. At first sight of Sergio, Michele senses that this man is pure evil. As Michele lay in bed, he instantly lost concentration when Sergio walked in the room. With the help of his courage and a dash of curiosity, Michele discovers that Sergio’s intentions are clear, as he finds the gun next to Filippo’s exercise book. Michele’s fears further escalate when he discovers that even his parents are involved in the plot.
Ammaniti depicts an adult world that represents unbelievable cruelty against mankind. The fathers exploit and humiliate a small innocent boy because of their own desire for revenge.
Filippo is kept in a very cruel condition. He is covered in his own bodily waste. He is starving; they threaten to cut off his ear. In this regard, Felice is more cruel and coercive than Skull. As Ammaniti suggests, when the adult world is devoid of moral leadership, the father is capable of killing his son – with or without his knowledge.
As Ammaniti suggests, whenever our role models, such as our parents or figures in a position of authority fail us, there are serious consequences for our sense of self, our confidence and our pride and dignity. Such a failure threatens our moral stability and our value system and leads to confusion. Moral ambiguity haunts Michele: “Papa is the bogeyman. By day he was good, but at night he was bad.” (87) Papa exploits the situation to improve his family’s standing in life and to find a better life for his children. Initially, he believes he can buy Michele with the promise of a better life, and with the gift of the most beautiful bike in the store.
“Papa is the bogeyman”. Michele fears his father because he tells him to betray his friend, Filippo. “By day he was good, but at night he was bad.” Such moral ambiguity tears Michele’s world apart and leads to a personal crisis. Papa’s “voice” should be the voice of morality, righteousness and good conduct. Instead the “voice” becomes a warning of danger and violence. “If you go back there they’ll kill him. They’ve sworn it.”
This paternal order contradicts Michele’s promise to Filippo which haunts him. He feels compelled to return to save Filippo but he becomes overwhelmed by the monsters, which are linked to the old man, who has masterminded the kidnap. “I felt as if a thousand eyes were watching me.” He sees “earth giants”, which belong to the “lords of the hill”. At first, he submits to his father’s orders and temporarily betrays his friend.
We all experience dilemmas and obstacles when growing up. Some obstacles are more severe than others. How we confront and deal with these dilemmas is critical to our self-esteem and our confidence in ourselves and in the world. In this regard, Michele is placed in a moral dilemma between loyalty to his friend, which involves moral compunction and his duty and his promise to his father, which haunts his conscience. His father, who should be a rock of morality, guidance and honesty, becomes the voice of corruption.
In Michele’s case, he faces a dilemma that is practically impossible to solve. At the very least, he needs to take the least traumatic path. By choosing Filipo he maintains his promise. He can at least retain his dignity and confidence. He shames the father, and completely destroys their relationship, but perhaps he recognizes that it was always fragile from the moment he recognized that the father was involved.
However, in choosing to return to the hills, Michele shows enormous courage and defies his father in favour of his principles. He places himself in grave danger and is shot by his father.

domain: library


text:
Cooking Lesson: From the Market to the Table in Florence
Learn to cook like an Italian at a 4-hour market to table cooking class, and enjoy a Tuscan meal in Florence.
Visit to the Central Market of Florence with a local Chef
Cooking class with local receipts
A full Italian meal from appetizer to dessert
Local lunch tasting
All necessary ingredients
Lunch with drinks (wine or soft drink)
Cancellation policy: We will charge a cancellation fee of 100% if booking is canceled 2 days or less before the event.
Please note: Minimum age is 4 years old. The tour starts from the meeting point in Florence.
Visit the central food market with your chef, before learning tips and techniques to make a full Italian meal that you can recreate at home. What if returning from Italy you could show your family and friends, not only pictures but your cooking skills too? Wouldn’t it be great to impress them with a typical Italian meal you cooked yourself? Join our professional cooking class! You will gain unique techniques and the best tips to make a perfect Italian meal! The class will start with a visit to the market: our Italian chef will help you choose the best ingredients. Once you get everything you need you’ll reach the cooking school where you’ll start to prepare the meal. During the four-hour lesson, you will prepare a full Italian meal, from appetizer to dessert. We will first prepare all the needed ingredients by washing and cutting them. Our chef will show you how to make fresh pasta from the raw ingredients and you will have the chance to knead it yourself! You will also cook the sauce (Tomato, Ragù Bolognese or Pesto), ‘bruschetta’ as a starter, as well as a dessert! Our chef will show you how to decorate your plate and you will finally have lunch with the amazing dishes you made. Lunch will be accompanied by a local wine, water, or soft drink as you prefer. At the end of the class, you will also get a Participation Certificate signed by the chef, as well as the recipes for the dishes you made. Then you can go home and show off to all your friends and families your great Italian cooking skills. In the end, you will be driven back to the departure place in the center of Florence.

domain: food


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Microsoft has announced that its recent trial that saw Xbox 360 users participate in a mock election via Xbox Live saw nearly 100,000 gamers participate – with Democratic candidate Barack Obama winning a landslide victory.
The experiment, which has been running since August 25th, allowed gamers to vote via their controller. Obama won 43 per cent of the vote, with Republican rival John McCain capturing 31 per cent.
Microsoft points out that the sample included in its trial is bigger than that seen in many recent polls combined – including the likes of NBC and CNN.
As well as participating in the poll, video coverage of the recent Democratic and republican conventions were downloaded by almost 25,000 users.

domain: news


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domain: game


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Сосна Лённрота —
Визитной карточкой поселка Калевала стала «Сосна Лённрота». Это, пожалуй, один из самых оригинальных памятников, известных не только в Карелии, но и далеко за ее пределами. По преданию, под этой сосной в начале XIX века калевальцы, взявшись за руки, пели долгие свои руны. Под этой же сосной калевальские старики пели Э. Лённроту, собирателю рун и создателю «Калевалы» и сосну эту люди стали называть Сосной Лённрота — по имени человека, сохранившего для людей калевальское сокровище. В давние времена был в Калевале мыс, густо поросший соснами. Самая старая сосна как раз и была той самой, под которой собирались руны. Когда построили плотину на реке Куйто, воды начали размывать песчаные берега, а после того, как здешний песок понадобился для строительства, и вовсе вековые сосны погибли. По легенде во время войны снаряд ранил старейшую сосну и ветер одолел раненное дерево вовсе. Так, практически незаметно ни для кого, чуть было не погибла легендарная сосна Лённрота, но нашлись люди, которые сумели спасти дерево. Они принесли вершину её с разлапистой кроной к Дому культуры и укрепили. Затем сосна потеряла крону, но остался кручёный ствол, отполированный ветрами, стоит он и поныне зацементированный в землю и серебрится в лучах солнца.
По материалам: Панкратов С., Поморцев Б. Север в душе моей. — Петрозаводск: Карелия, 1986. — 151 с., с29,30
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Flow cytometry is an appealing technique because it enables users to analyze multiple cell types in a single experiment. In the early days of flow cytometry, when cytometers had one or two lasers, and only a limited number of fluorescent probes existed, complex staining panels may have had only four colors.Read the full article »
Flow cytometry is a well-established approach used in numerous clinical assays across the globe. But like most assays used in a clinical setting, flow cytometry assays typically need to be validated before they can be used for diagnostic or clinical research purposes.
Assay validation is done to demonstrate that a method is accurate, specific, reproducible and robust over a designated range of measurement. Flow cytometry is considered an analytical method, and according to the FDA, flow cytometry assays need to meet specific analytical method validation criteria. A validated assay can then be used in situations requiring GLP/GCP (good laboratory/clinical practice) compliance, like a clinical trial or routine diagnostic test.Read the full article »
Receptor occupancy (RO) assays are a powerful tool for pharmacokinetic/pharmacodynamic evaluations of candidate drugs and biologics. RO assays can also be used toward dose selection for candidate molecules being evaluated in clinical trials. Flow cytometry-based RO assays are currently being used in many sectors of biopharmaceutical drug development. Consider these five things to know about RO assays if you are planning to use this type of assay in your preclinical research.Read the full article »


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