text:
As you know me quite well by now, I prefer to give every single look I wear during FASHION WEEK the attention and spotlight it deserves, by dedicating one full blog post around it, when possible of course. So here I present to you the one I wore on my 3rd day of Milan Fashion Week. A lot of you saw it on Facebook and on my Instagram page – and granting I LOVE all the others, this one totally encompassed my mood – and so many people I spoke to that day simply loved. Whoaa!! Made my day all the more exhilarating!
I wore it wholeheartedly to an interview of an amazing Italian jewellery brand that I will soon go into detail about, I wore it to the 3 backstage sneak peaks with MAC Cosmetics, to the haute couture show by Curiel and also to Tommy Zhong’s presentation.
It consisted of an embroidered pale pink shirt, a long maxi skirt, a small brown suede bag and velvet hat – all by Italian high fashion brand MANGANO. Earrings are by Vitti Ferrie and my sunnies are Valentino. I felt so comfortable, all day long!
Photos: Justin Ciappara
Tommy Zhong’s presentation held at Sala delle Cavallerizze in Via Olona 6bis featured the Spring/Summer 2018 Collection and it was supported by the Camera Nazionale della Moda Italiana, and the Fashion Avenue Academy.
This contemporary fashion collection focused on textile surface manipulation to present a landscape of silhouettes created from blocks of colour in a refined palette of black and white and interrupted by a burst of orange or pink. We saw an interesting collection with a metropolitan feel to it. The concrete surroundings exposed the chasms of fragility echoed in the juxtapositions of fabrics and raw finishes.
As you can see from the photos we took, each piece is completely unique and individual. My favourites are the black maxi dress and the colourful jacket worn by the beautiful red haired model. I also loved the black and white jacket. I also found the shoes to be lovely, available in both black and white patent. Enjoy these lovely photos!
The brand Tommy Zhong for those of you who are not familiar with it, is a brand that represents the union of Chinese and Western cultures: Jiagi Zhong of China working together with Jennifer Nelson who, while based in London, has a strong interest in Asia. Together their combined talents and expertise produce a wonderful chemical reaction through the innovation of fabrics and the language of art. Well done for this exhibition and thank you to Bookin’ Agency in Milan and Australian Gold Malta for organising our passes to this show!
Fashion & Travel Blogger at www.graziellecamilleri.com
A blog brought to you with love from Milan Fashion Week 2017
During the show .. we spotted one of the designers ! Here she is > Jennifer Nelson


text:
Last year, I boldly went where I had never gone before. In the beloved tragic French opera /Carmen,/ there is a gorgeous instrumental melody in the overture to Act III, an air that appears nowhere else in the opera. I wondered, Why has it never been given words and made into a song of its own? As my tenth wedding anniversary approached, I did exactly that as a surprise gift to my opera-loving husband.
Never having composed lyrics for music before, I struggled in secret for a very long time, trying to count the beats in the measures and musical phrases that make up the melody, in order to know how to write the words. Despite frustration, I pressed on, goaded by another consideration: in the opera, this melody starts with a flute and harp duet. It so happens that I am a harpist, having played professionally for 13 years a long time ago. If only I could create the lyrics, I could hire the soprano who sang at our wedding ten years before, and perform this with her at our anniversary party. So went my fantasy. If only I could get past this block.
Finally, I thought of a solution. Instead of trying to memorize the correct number of half-notes and quarter-notes and measures per phrase, and then squeeze English words into them, I said to myself, “Just think of words in French as a way to learn the rhythm and measures. Then it will be easier to hold them in mind while trying to think of English words.”
Well, that broke the logjam! The French words just flowed and they were perfect. Luckily, the ideas also translated seamlessly into English words that fit the music. I kept my secret until the very last minute and our harp-soprano world premiere (thank you, composer Georges Bizet) was a joy to share.

domain: artistic


text:
The olfactory bulb is a protruding structure formed at the rostral end of the telencephalon. Pax6-mutant mice and rats lack the olfactory bulb and, instead, develop an olfactory bulb-like structure at the lateral part of the telencephalon. Here, we report that ectopic formation of the olfactory bulb-like structure in these mutants is caused by the abnormal migration of mitral cell progenitors, which first differentiate within the olfactory bulb. Cell-tracing experiments in whole embryos in culture indicate that, in the mutants, the mitral cell progenitors that originate from the rostral part of the telencephalon migrate caudally toward the lateral part of the telencephalon. Cell transplantation demonstrates that the abnormal cell migration is not autonomous to the mitral cell progenitors themselves. The mislocation of the olfactory bulb in the mutant is not caused by loss of olfactory nerve innervation. Furthermore, transfection of a Pax6-expression vector to the mutant telencephalon restores the normal migration of mitral cell progenitors. These results provide evidence that Pax6 is required to position the mitral cell progenitors at the rostral end of the telencephalon.
The olfactory bulbs (OBs) are structures that protrude from the rostral telencephalon and function as the primary processing center for odor information. The OB has a laminar structure, consisting of several types of neurons and glial cells (Hinds,1968; Pinching and Powell,1971). Mitral cells, which differentiate first among the OB neurons, receive direct axonal innervation from olfactory receptor neurons in the olfactory epithelium and their axons project to the olfactory cortex(Hinds and Ruffett, 1973). Several interneurons also have important roles in local circuits within the OB. However, it is unclear how the OB forms at the precise position of the rostral telencephalon.
Numerous lines of evidence demonstrate that establishment of regional identity and specification of cell types in the telencephalon is regulated by several transcription factors (Shimamura et al., 1995; Rubenstein et al., 1998; Campbell,2003). Pax6, a member of the Pax family of transcription factors, has crucial roles in various developmental processes of the telencephalon including dorsal-ventral and anterior-posterior patterning,specification of neuronal subtypes, neuronal migration and axonal projection(Engelkamp et al., 1999; Kawano et al., 1999; Stoykova et al., 2000; Bishop et al., 2000; Bishop et al., 2002; Yamasaki et al., 2001; Yun et al., 2001; Pratt et al., 2002).
Pax6 homozygous-mutant mice (Sey/Sey) lack OB protrusion but still have olfactory bulb-like structures (OBLSs) at the lateral side of the telencephalon (Lopez-Mascaraque et al., 1998; Jimenez et al.,2000; Hirata et al.,2002). This may indicate that Pax6 function is required to determine the position where the OBs should form within the telencephalon. However, the mechanisms by which Pax6 determines OB position in the developing telencephalon and the primary cause of ectopic formation of the OBLS in the Pax6 mutant are unknown.
In this study, we investigated how the OBLS develops ectopically in Pax6-mutant rats (rSey2/rSey2) using long-term culture systems of whole embryos and brain explants. Cell-tracing analyses revealed that misposition of the OBLS was not caused by ectopic generation of mitral cells, but by abnormal migration of mitral cell progenitors in the telencephalon of Pax6 mutants. Transplantation of mitral cell progenitors showed clearly that abnormal cell migration was caused by non-cell autonomous defects of the mitral cell precursors. Removal of olfactory innervation did not affect the migration pattern of the mitral cell progenitors, indicating that the mutant phenotype is not caused by the impairment of olfactory nerve innervation. Furthermore, transfection of exogenous Pax6 into the mutant telencephalon restored abnormal cell migration, implying that Pax6 function is required within the telencephalon. These results demonstrate that loss of Pax6 function in the telencephalon disrupts a positional cue that is required to direct the mitral cell progenitors to the rostral end.
Materials and methods
Pregnant Sprague-Dawley (SD) rats were purchased from Japan Charles River(Tokyo, Japan). Heterozygous Pax6 mutants on SD background rats(rSey2) (Osumi et al.,1997) were intercrossed in our laboratory to obtain homozygous embryos, which are distinguished from wild-type and heterozygous littermates by an eyeless phenotype. GFP-transgenic rats [TgN(act-EGFP)Osb4](Ito et al., 2001) were provided by Dr Okabe and crossed with the rSey2 strain. Midday on the day a vaginal plug was found was determined as embryonic day 0.5(E0.5). The following experimental procedures were approved by the Committee for Animal Experiment of Tohoku University Graduate School of Medicine.
Embryos were fixed with 4% paraformaldehyde and sectioned with a Cryostat(CM3050, LEICA). The sections were immersed in 5% skimmed milk in TBST(tris-buffered saline plus 0.01% tween20) for 30 minutes, and incubated with anti-Neuropilin 1 (rabbit polyclonal, a gift from Dr Hirata), anti-calretinin(mouse monoclonal, CHEMICON), anti-GAD67 (rabbit polyclonal, CHEMICON),anti-bromodeoxyuridine (BrdU, mouse monoclonal, Beckton-Dickinson) antibodies at 4°C overnight. Cy3-conjugated anti-rabbit or anti-mouse IgG antibody(Jackson) was used as the secondary antibody. After washing with TBST, the sections were examined under the fluorescent microscope (Axioplan-2, Zeiss)equipped with a cooled CCD camera (Roper). Confocal images of GFP/Cy3 double-positive cells were acquired using a Leica TCS NT confocal microscope and 3D images constructed with Leica confocal software. Whole-mount immunostaining of the telencephalon was performed according to previous procedures (Nomura et al.,1998). For detection of BrdU, sections were treated with 2M HCl at 37°C for 30 minutes before immunohistochemistry.
In situ hybridization
Digoxigenin (DIG)-labeled RNA probes were transcribed from neuropilin 1(Kawakami et al., 1996), Pax6 (Osumi et al.,1997), netrin G1 (Nakashiba et al., 2000) cDNAs that had been subcloned in pBluescript using the DIG RNA labeling kit (Roche). The hybridization procedures have been described previously (Osumi et al.,1997; Takahashi and Osumi,2002). Hybridization signals were detected with AP-conjugated anti-DIG antibody (Roche) and nitroblue tetrazolium,5-bromo-4-chloro-3-indol-phosphate (Roche).
Cell labeling and cell transplantation in cultured embryos
The experimental procedures of whole embryo culture (WEC) and cell labeling have been described previously (Ishii et al., 2000; Takahashi and Osumi, 2002; Takahashi et al.,2002). To label the progenitors of the olfactory bulb neurons, DiI(D-282, Molecular probe) solution dissolved in dimethylfomamide was microinjected with a fine-tipped glass needle (Shutter Instrument Co) into the rostral end of the telencephalon. To analyze the birthdate of the labeled cells, embryos were cultured in medium containing 40 μM BrdU (Boehringer Mannheim) for 30 minutes. In whole brain culture (WBC), brain tubes including the forebrain, midbrain and hindbrain regions were isolated by removing mesenchymal tissues and cultured for 24 hours in rotating bottles filled with culture medium for WEC (100% rat serum containing 2 mg ml-1 glucose and 0.025% antibiotics) in 60% O2. Telencephalic organ culture(TOC) was performed as described previously(Sugisaki et al., 1996). Briefly, after 48 hours in WEC or WBC, the telencephalic hemispheres were dissected out, treated with 0.2% collagenase IV to remove the pia matter, and cultured on a collagen-coated membrane filter (transwell-COL 3492, Coaster)for 2-3 days at 37°C, in 0.25% CO2. To transplant OB neuronal progenitors, small fragments of the rostral telencephalon of Pax6homozygote embryos and their littermates on GFP-transgenic background were dissected out, and treated with 0.2% collagenase to remove the mesenchyme. The fragments were further dissociated into single cells with 0.25% trypsin, and∼50-100 cells microinjected into the rostral telencephalic wall of host embryos.
Gene transfer by electroporation
Details of electroporation in cultured embryos were described previously(Takahashi et al., 2002). After 2 hours of preculture in WEC, embryos were transferred into tyrode's solution, of 0.1 μl plasmid vector solution (either pCAX-GFP or pCAX-Pax6)(Takahashi et al., 2002) and microinjected into the telencephalic vesicle. Square pulses (70V, 5 Hz) were delivered into the embryos using an electroporator (CUY21, NEPPA GENE) and tweezer-type electrodes (CUY-650).
The OBLS is ectopically formed in the Pax6 mutant rat telencephalon
Previous studies have shown that Sey/Sey embryos have OBLS at the lateral side of the cortex(Lopez-Mascaraque et al.,1998; Jimenez et al.,2000; Hirata et al.,2002). To test whether rat rSey2/rSey2 embryos also exhibit a similar phenotype, we examined expression of several markers for OB neurons. Neuropilin 1 (Nrp) is strongly expressed in mitral cells(Kawakami et al., 1996; Sugisaki et al., 1996; Hirata and Fujisawa, 1997),one of the projection neurons that differentiated first, in the developing OB of wild-type rat embryos at E16.5 (roughly corresponding to E14.5 of mouse embryos) (Fig. 1A,E). In the OB, Nrp immunoreactivity was localized at the external plexiform layer, which consists of the neuropiles of mitral cells(Fig. 1A,I). In contrast, a Nrp-positive structure was observed on the lateral side of the rSey2/rSey2 telencephalon(Fig. 1B,K), similar to that in murine Sey/Sey embryos (Hirata et al., 2002). This Nrp-positive structure in rSey2/rSey2 telencephalon also expressed netrin G1 which marks mitral cells in the developing OB(Fig. 1G,H)(Nakashiba et al., 2000). In the OB and OBLS, Nrp or netrin G1-positive mitral cell did not express Pax6(data not shown). Next we examined whether interneurons exist in the OBLS using several markers. Calretinin-immunopositive cells, which are corresponding to the periglomerular cells, are located at the glomerular layer of the developing OB (Kosaka et al.,1995) (Fig. 1I). Calretinin-positive cells were also observed in the superficial area of the OBLS (Fig. 1K). In the OB,GABAergic and dopaminergic interneurons express glutamic acid decarboxylase(GAD67) and tylosine hydroxylase (TH), respectively.(Esclapez et al., 1994; Kosaka et al., 1995). GAD67-immunopositive cells located at the glomerular and the external granule cell layers in the OB (Fig. 1J), while they distributed at the peripheral area of the OBLS,adjacent to the Nrp-positive area (Fig. 1L). We could not detect any TH-immunopositive cells in the OBLS,similar to the previous report of mouse Pax6 mutant(Jimenez et al., 2000).
Taken together, these results indicate that the OBLS is formed at the lateral side of the cortex in rSey2/rSey2embryos as in the case of the mouse Pax6 mutant. Because the mitral cells are first neurons to differentiate in the OB(Hinds, 1968), we focused on the mitral cell development in the following experiments.
Cell tracing of mitral cell progenitors in wild-type and Pax6-mutant rats
Because the OBLS developed at an ectopic position in the Pax6mutant telencephalon, we hypothesized two possibilities to account for the defect in OB formation. One is that the origin of the mitral cells may be altered in the mutant. A previous study suggested that the mitral cells are derived from the dorsal part of the telencephalon(Bulfone et al., 1998; Puelles et al., 2000). Thus,the mitral cells of the Pax6-mutant OBLS differentiate in an ectopic position of the telencephalon compared to wild type. The other possibility is that the mitral cells of the mutant embryo migrate abnormally and colonize in an ectopic position to form the OBLS. To test these possibilities, we performed cell-tracing analyses in embryos in culture and compared the origin and migration patterns of the mitral cells in wild-type and rSey2/rSey2 embryos(Fig. 2A). Because mitral cells are born at ∼E12-E13 in rat embryos(Bayer, 1983), we labeled a part of the telencephalon at E12.5 and cultured the labeled embryos for 48 hours.
When the rostral-most telencephalon of wild-type embryos (n=4) was labeled by the lipophilic fluorescent dye DiI(Fig. 2C), most labeled cells stayed in the area close to the dye-injection point(Fig. 2B,E). Only a few of labeled cells were detected in the lateral part of the cortex. In contrast,when a similar area was labeled in rSey2/rSey2embryos (Fig. 2D, n=4), a large number of labeled cells were observed to migrate caudally toward the lateral part of the telencephalon(Fig. 2B,F). The area in which DiI-labeled cells accumulated seemed to correspond to the area where the OBLS would form later in development (Fig. 2F, arrowheads).
Differentiation of the mitral cells did not occur in embryos just after WEC(data not shown). To examine whether the labeled cells contribute to the OB or the OBLS and differentiate into mitral cells, we isolated telencephalons and cultured them as explants. After 48 hours of WEC followed by 3 days of telencephalic organ culture (TOC), a protruding OB, marked by calretinin,developed at the rostral end of the telencephalon of wild-type embryos(Fig. 3E″). Nrp and netrin G1 were also detected in this OB(Fig. 3F″ and data not shown). Therefore, this long-term culture system seemed to be suitable for examining the development of mitral cells from their origin to initial differentiation. Next, instead of DiI injection, we electroporated a GFP-expression vector to focally label the rostral telencephalon because GFP-fluorescence is stable after the immunostaining using detergents. When the GFP gene was introduced into the rostral part of the dorsal telencephalon (i.e. the Pax6-positive area) of wild-type embryos,GFP-positive cells stayed in the rostral part of the telencephalon, even 48 hours after electroporation (Fig. 3A-D). After 3 days of TOC, most GFP-positive cells contributed to the OB evaginated from the rostral tip of the telencephalic explants(Fig. 3E-E″). GFP-labeled cells within the protruding OB expressed Nrp, indicating that they differentiated into mitral cells (Fig. 3F-F″,G-G″). Of the GFP-positive cells,69.8±6.2% were Nrp-positive in the OB (n=4). To further confirm that cells that express GFP were mitral cells, we examined their birthdate by labeling with BrdU. GFP-transfected embryos were cultured in BrdU-containing medium for 30 minutes, 6 hours after electroporation(n=3). This corresponds to E12.75. After a further 3 days of TOC,∼50% of GFP-positive cells in the OB were labeled with BrdU(Fig. 3H-H″). Because the peak of mitral cell neurogenesis occurs at ∼E12-E13 in rat embryos(Bayer, 1983), these BrdU/GFP-positive cells are considered to be mitral cells. Taken together, we conclude that the mitral cells originate from the rostral-dorsal telencephalon in normal development.
Next, we asked whether the cells originating from the rostral telencephalon of rSey2/rSey2 embryos could form the OBLS. When the rostral end of the dorsal telencephalon of E12.5 mutant embryos was labeled with GFP, a large number of labeled cells migrated caudally toward the lateral part of the telencephalon within 48 hours of WEC (data not shown), as observed in the DiI labeling (Fig. 2D). After 3 days of TOC, calretinin-positive and Nrp-positive OBLS developed at the lateral surface of the mutant telencephalon, which included the GFP-positive cells derived from the rostral part of the dorsal telencephalon (Fig. 4D-D″,E-E″). Of the GFP-positive cells,64.7±1.5% expressed Nrp in the OBLS, indicating that they differentiated into mitral cells (n=4)(Fig. 4F-F″). We detected no GFP-positive cells in the OBLS when other areas of the telencephalon were labeled (data not shown). We also performed pulse labeling with BrdU to confirm the birthdate of the GFP-positive cells (n=3). After 30 minutes of pulse labeling, ∼50% of GFP-expressing cells within the Nrp-positive OBLS were also labeled with BrdU(Fig. 4G-G″), indicating that they are mitral cells. Taken together, these data indicate that the origin of mitral cells is not altered in rSey2/rSey2 mutants. However, in these mutants,the mitral cell progenitors migrated caudally toward the lateral part of the telencephalon and formed the OBLS in an ectopic position.
Abnormal migration of mitral cell progenitors results from non-cell autonomous defects of mitral cells themselves
Pax6 is expressed in the neuroepithelium of the dorsal telencephalon (Stoykova and Grüss,1994), including the origin of the mitral cells. Pax6also regulates the development of the lateral part of the cortex(Hirata et al., 2002) and there are severe defects in formation of the olfactory epithelium and subsequent differentiation of neurons in Pax6-mutant mice and rats(Hogan et al., 1986; Hill et al., 1991; Fujiwara et al., 1994; Grindley et al., 1995). These lines of evidence led us to question whether the impaired migration in rSey2/rSey2 mutants results from a defect that is cell-autonomous or non cell-autonomous to the mitral cell progenitors. To test these possibilities, we isolated neuroepithelial cells containing the mitral cell progenitors from the rostral telencephalon of E12.5 rSey2/rSey2 embryos on a GFP-transgenic background (Ito et al., 2001),and transplanted them into either wild-type or heterozygous littermates, and vice versa. After transplantation, whole embryos were cultured for 48 hours followed by 3 days of TOC (Fig. 5A). The transplanted cells were easily distinguished in the hosts by their GFP-fluorescence.
When the rostral-most telencephalic cells of E12.5 homozygous embryos were transplanted into the same region of either wild-type or heterozygous(rSey2) embryos, most of the donor cells localized at the rostral part of the telencephalon and contributed to the host OB(Fig. 5B,C). Some of the donor cells expressed Nrp, indicating that they differentiated into mitral cells(Fig. 5C′). In these transplantation experiments we did not observe mutant donor cells that migrated toward the caudal part of the telencephalon(Fig. 5F). In contrast, when cells isolated from the rostral part of the telencephalon of E12.5 wild-type and heterozygous embryos were transplanted into the corresponding region of the telencephalon of homozygous mutants, many GFP-positive cells migrated caudally (Fig. 5D,E). These donor cells expressed Nrp and contributed the OBLS that formed in the lateral part of the mutant telencephalon (Fig. 5E′). This migration pattern of the donor cells resembled that of homozygous mitral cell progenitors labeled with either DiI or the GFP gene (Figs 2, 4). These results indicate that abnormal migration of the mitral cell progenitors is not caused by cell-autonomous defects of the mitral cells themselves, but by an impaired migratory environment in the mutant telencephalon.
Impaired migration of the mitral cell progenitors is not caused by lack of olfactory nerve innervation
We next examined whether penetration of the olfactory nerve into the brain affects mitral cell movement because development of the olfactory epithelium is also impaired in Pax6 mutants(Hogan et al., 1986; Hill et al., 1991; Fujiwara et al., 1994; Grindley et al., 1995). To address this, we isolated brain tubes, by removing surrounding tissues containing the olfactory epithelium and nerve, and cultured them to access the localization of the mitral cells. Primary innervation of the olfactory nerve commences at E13 in rat embryos (Gong and Shipley, 1995). Thus, we isolated brain tubes from E12.5, and cultured them for 24 hours in rotating bottles (WBC) and, subsequently, as a TOC for 2 days (Fig. 6A). After a total 3 days of culture, specimens were stained for netrin G1.
When wild-type brains (n=7) were cultured without olfactory nerve innervation, netrin G1 expression was observed at the rostral part of the telencephalic explants (Fig. 6B, arrowhead). There were no netrin G1-positive cells at the lateral part of the telencephalon. This strongly supports a recent report that the specification of mitral cells occurs without olfactory nerve innervation(Long et al., 2003). In mutant explants (n=5), a cluster of netrin G1-positive cells was observed at the lateral part of the telencephalon, indicating that abnormal migration of mitral cells was reproduced during these culture conditions(Fig. 6C). Taken together,these results indicate that abnormal migration of mitral cell progenitors in the Pax6 mutant is not caused by a lack of olfactory nerve innervation, but is intrinsic to the telencephalon.
Introduction of exogenous Pax6 into the mutant telencephalon restores the normal migration of mitral cell progenitors
To further confirm that Pax6 function is required to control migration of mitral cell progenitors in the telencephalon, we examined whether the mismigration of the mitral cell progenitors is rescued by transfection of an exogenous Pax6-expression vector into the mutant telencephalon.
Either GFP alone or GFP plus Pax6 expression vectors were introduced into the E12.5 mutant telencephalon by electroporation. After WEC followed by TOC, we examined the localization of netrin G1-positive cells in the mutant telencephalon. GFP fluorescence showed that exogenous genes were expressed throughout the rostral-half of the telencephalic neuroepithelium, including the origin and the migratory pathway of the mitral cell progenitors (Fig. 7A). As expected, when we introduced the GFP gene alone into the mutant telencephalon, accumulation of netrin G1-positive cells was observed at the lateral surface of the telencephalon (8/8 cases)(Fig. 7B, Fig. 1D, arrowheads). However,when the Pax6-expression vector was electroporated into the mutant hemisphere, netrin G1-positive cells accumulated at the rostral-most telencephalon (4/10 cases; Fig. 7C, arrowhead). Nrp was also expressed in the area containing netrin G1-positive cells (Fig. 7E). Nrp-positive cells were sorted from those expressing exogenous Pax6 as is seen in wild-type embryos(Fig. 7E,F, and data not shown). In 3/10 cases, clusters of netrin G1-positive cells were observed in the rostral-most part of the cortex, but there were also some cells at the lateral side of the cortex (Fig. 7G). However, the localization of netrin G1-positive cells was unchanged (Fig. 7G) in three other cases, probably because of limited expression of the Pax6 gene. These results demonstrate that transfection of exogenous Pax6restored normal migration to mitral cell precursors in the mutant telencephalon. Taken together, Pax6 function in the telencephalon is necessary to retain mitral cells in the rostral-most part of the telencephalon.
In this study, we revealed that ectopic formation of the OBLS in the Pax6 mutants is caused by abnormal migration of mitral cell progenitors. The migration defect was further proved to be caused by lack of Pax6-function in the mutant telencephalon (i.e. in the environment in which mitral cells migrate) rather than a defect in mitral cell progenitors. Furthermore, removal of olfactory innervation and introduction of exogenous Pax6 to the mutant telencephalon demonstrated that Pax6functions are required in the telencephalon to regulate the migration pattern of the mitral cell precursors. These findings support a model in which Pax6 controls positional information that is responsible for positioning the mitral cell progenitors in the telencephalon.
Pax6 controls the positioning of mitral cell progenitors in the telencephalon
The most striking result in the present study is the abnormal migration pattern of mitral cell progenitors in the Pax6 mutant. In these mutants, the mitral cell progenitors derived from the rostral region migrated caudally and clustered at the lateral surface of the telencephalon. This indicates that the ectopic formation of the OBLS in the mutants is not caused by an alteration of the mitral cell origin, but by the abnormal migration of mitral cell progenitors. It has been reported that Pax6 regulates migration of cerebellar granule cells, which strongly express Pax6,in a cell-autonomous manner (Engelkamp et al., 1999; Yamasaki et al.,2001). By contrast, mitral cells originate from a Pax6-positive, dorsal part of the telencephalon, but the migrating precursors and differentiated mitral cells do not express Pax6(Fig. 2 and data not shown). It is, thus, unexpected that Pax6 is required for the mitral cell progenitors to autonomously control their migration patterns, an effect that was proved by cell transplantation experiments. Such a cell non-autonomous function for Pax6 in the control of neuronal migration has also been observed for the migration of neural crest cells from the midbrain towards the frontonasal region (Osumi-Yamashita et al., 1997; Nagase et al.,2001). Therefore, Pax6 functions controls the migration of neuronal cells in both cell-autonomous and non-autonomous ways, depending on the developmental context.
It has long been believed that projection of the primary olfactory nerve to the rostral telencephalon affects development of the OB. In frog and chick embryos, removal of the olfactory epithelium abolishes OB development(Graziadei and Monti-Graziadei,1991; Wang et al.,2001). In our study, however, ablation of olfactory innervation did not alter the location of the mitral cells within the telencephalon of wild-type rat embryos. This excludes the possibility that penetration of the olfactory nerve directs the migration of the mitral cell progenitors. However,the OB in the wild-type cultured brain and the OBLS in the Pax6mutant do not evaginate from the telencephalon. It may, thus, be possible that olfactory innervation controls further protrusion of the OB in later development. It has been reported that the frontonasal region may play an essential role for OB morphogenesis(LaMantia et al., 1993). Several types of secreted molecules such as retinoic acid (RA), FGF8 and BMP4 are expressed at the frontonasal region(LaMantia et al., 1993; LaMantia et al., 2000), and disruption of RA- and FGF signaling leads to severely compromised OBs(LaMantia et al., 1993; Anchan et al., 1997; Hebert et al., 2003; Garel et al., 2003). Therefore, interaction between the frontonasal region and the rostral part of the telencephalon, through the activities of these molecules, could be essential for OB protrusion.
Abnormal migration of mitral cell progenitors in Pax6 mutants was rescued by transfection of exogenous Pax6 into the mutant telencephalon. This indicates that mislocation of the OBLS in Pax6mutants is caused by loss of Pax6 function in the mutant telencephalon. Recent studies have reported that Pax6 regulates rostral-caudal patterning of the telencephalon (Bishop et al., 2000; Bishop et al.,2002; Muzio et al.,2002). Because the rostral-lateral area of the telencephalon,which expresses Pax6 strongly, is reduced in Sey/Seyembryos, Pax6 is thought to coordinate the rostral-lateral identity of the telencephalon (Bishop et al.,2002; Toresson et al.,2000; Yun et al.,2001). It has also been demonstrated that the rostral-caudal patterning of the telencephalon is influenced by FGF signaling(Fukuchi-Shimogori and Grove,2001; Storm et al.,2003; Garel et al.,2003). We also identified that expression of fgfrs is reduced severely in the telencephalon of Pax6 mutants, especially in the rostral area (T.N. and N.O., unpublished). FGF signaling is, thus, likely to be disturbed in the telencephalon of Pax6 mutants. These data support the idea that transfection of exogenous Pax6 restores an impairment in regional identity in the rostral area of the mutant telencephalon and re-establishes the positional information that regulates the migration patterns of mitral cell progenitors. However, we cannot rule out the possibility that restoration of the cell migratory pattern in Pax6-transfected embryos is unrelated to the initial defect in Pax6 mutants, but is caused by a secondary event that is induced by transfection of exogenous Pax6. To solve this problem, it will be necessary to identify molecules downstream of Pax6 that directly control the migration pattern of mitral cell precursors.
Mechanisms controlling migration patterns of mitral cell progenitors
What mechanisms could be responsible for controlling cell migration patterns of mitral cell precursors? Because mitral cell precursors migrate caudally towards the lateral part of the mutant telencephalon, it might be possible that migratory cue(s) that inhibit or attract migration of mitral cell precursors are absent and/or appear ectopically in the lateral region of the telencephalon of Pax6 mutants. Pax6 regulates the expression of cell adhesion molecules including R-cadherin, which is responsible for maintaining the compartment boundary between the dorsal and ventral parts of the telencephalon(Stoykova et al., 1997; Inoue et al., 2001; Tyas et al., 2003). Thus, it might be possible that contact-dependent, cell-cell interaction between mitral cell precursors and cortical neuroepithelial cells is important for positioning the olfactory bulb in the telencephalon. However, we could not restore the position of the OBLS using an expression vector to overexpress R-cadherin in the mutant telencephalon (data not shown). Also, we could not identify altered expression of neuronal guidance molecules, such as netrin 1,slit 2 and semaphorin 3A (T.N. and N.O., unpublished), implying that other molecules are responsible for controlling mitral cell migration.
The mitral cells originate from the rostral-dorsal telencephalon
Many researchers have investigated the developmental origin of the OB, but it is still ambiguous whether the OB is derived from the dorsal or ventral telencephalon. Several morphological analyses have classified the OB as a ventral telencephalon-derived structure(Källen, 1962; Nieuwenhuys, 1998). By contrast, molecular analyses and genetic approaches indicate that mitral cells originate from the dorsal telencephalon(Bulfone et al., 1998; Puelles et al., 2000). In the present study we performed cell tracing in WEC and TOC, which revealed directly that the mitral cells are derived from the rostral part of the dorsal telencephalon. However, several cell tracing and genetic analyses indicate that interneurons of the OB are derived from the ventral telencephalon(Anderson et al., 1999; Wichterle et al., 2001; Nery et al., 2002). Thus, it is likely that the OB is a mosaic structure consisting of several types of neurons that have different origins within the telencephalon, as in the case of the neocortex (Wilson and Rubenstein,2000) and the olfactory cortex(Hirata et al., 2002). In Pax6 mutants, the OBLS consists of the projection neurons and different types of interneurons probably corresponding to the perigromerular cells and the granule cells (Fig. 1). The question of whether the origins and migratory pathways of these interneurons are impaired in the mutants remains to be clarified. Precise fate mapping of the OBLS interneurons in Pax6 mutants is required to further understand the roles of Pax6 in positioning the different types of OB neurons in the telencephalon.
We thank Drs Hajime Fujisawa, Yoshio Wakamatsu and Joseph Henri Bayle for their valuable suggestions on the manuscript. We also thank Drs Masaru Okabe for providing GFP transgenic rats, Shigeyoshi Itohara for netrin G1 cDNA,Hajime Fujisawa and Tatsumi Hirata for Nrp cDNA and anti-Neuropilin 1 antibody, and Masatoshi Takeichi and Takayoshi Inoue for the R-cadherin expression vector. We are also grateful to Nao Kamata, Michi Otonari and Yumi Watanabe for technical support, and all other members of our lab for helpful advice. This work was supported by grants from Japanese Ministry of Culture,Sports, Science and Technology (No 13035003 and 14034201 to T.N.).

domain: literature


SKF D/WR6 Description :
We specialize in supplying original SKF D/WR6 bearing and provide customers with SKF D/WR6 bearing size, specifications and drawings for customer reference. My company's SKF D/WR6 bearing price concessions, adequate inventory, and other similar products are available for recommendation
Packaging Details :
1 . Industrial tube packing + cartons + pallets
2 . Individual colorful or white box + carton + pallets
3 . As the clients ' requirement
1 . Less than 45 KGS, we will send by express. (Door to Door, Convenient)
2 . 45 - 200 KGS , we will send by air transport . (Fastest and safest, but expensive)
3 . More than 200 KGS, we will send by sea . ( Cheapest and common use )
SKF D/WR6 bearing NSK Ball Bearing 6317 | Leader
Heavy Equipment Parts & Accs>Manufacturing Equipment Parts>Bearings>See more 6317 NSK Single Row Ball Bearing NSK Ball Bearing 6317 NSK Ball Bearing 6317 Please call us if you have any questions at 714-871-5558, [email protected] can pick up from Fullerton, California or Piscataway, New Jersey or we will ship via UPS Ground. Fullerton Office2320 E Valencia DrFullerton, CA 92831 Piscataway ...
SKF D/WR6 bearing TIMKEN W314PP | Leader
Leader Singapore is authorized dealers in interchange all bearing types, including TIMKEN W314PP McGill CAMROL Cam Followers fag nsk skf timken ina koyo bearing configurations and advanced products such as housed units, integrated bearing assemblies, precision assemblies and cam followers, radial bearings, and thrust bearings.
SKF D/WR6 bearing Mój wnuczek Nataniel w ostatnim tygodniu na końcu z
Mój wnuczek Nataniel w ostatnim tygodniu na końcu z głową kupił ciągnik steyr dane techniczne. After a bad meeting pimentense pimenta bueno with koffpeik/2 kaskö kaskinen / teuva I bought the cote d'or vineyard 700 piece jigsaw puzzle, louis jadot by louis jadot
SKF D/WR6 bearing Buy Military and Aircraft Parts, NSN, National Stock
Buy Military and Aircraft Parts, NSN Parts, Connectors and Electronic Components - Get fast quotes. Important: Please fill in all the information or we are unable to process your request! Fields marked with * are required Most components have a minimum order size of $ 250!
SKF D/WR6 bearing Spherical roller bearings - 22232 CCK/W33 -
In addition to the information provided on this page, consider what is provided under Spherical roller bearings.; For information on selecting the appropriate bearing for a given application, and interpreting the data on this page, refer to Bearing selection process.; For general information on rolling bearings, refer to General bearing knowledge.
SKF D/WR6 bearing Bearings WR6 (SKF)|WR-6 bearings Papua New
Bearings WR6 (SKF)|WR-6 bearings Papua New Guinea Easy Bearing Co., Ltd., the original SKF WR6 bearing can be delivered to you immediately. We also provide SKF WR6 Snap rings selection, installation, maintenance and replacement of bearings of various internationally renowned bearings.
SKF D/WR6 bearing TAF425220 bearing IKO
Model: TAF425220D:52 mmCr:29900 N C0r:56200 N Grease RPM:9500 1/minm:86.5 kg TAF425220 bearing IKO -TAF425220
SKF D/WR6 bearing SKF Startseite |
SKF’s automotive bearing factory in Tudela, Spain joins industrial bearing factory in Steyr, Austria as the Group’s first carbon neutral manufacturing sites 2020.02.17 Notice of Annual General Meeting
SKF D/WR6 bearing NSK|NJ2306ET|Bearings|bearings 2000 sales
NMB L950 ISB 6032ZZ PFI B1747D KOYO 22042RS SKF W619/2.5R NTN EC6206LLU SKF D/WR6 NMB R725. Get Best Price Skype. ToBearings professional team. ToBearings advanced automated bearing production line and testing equipment, higher than ISO 9000 standards and advanced technology, specializing in the production of high quality precision bearings ...


text:
- Aiims Jodhpur
All India Institute of Medical Sciences, Jodhpur
AIIMS Jodhpur is one of the 6 new AIIMS to be established in 2012 by the Ministry of Health & Family Welfare, Government of India under the Pradhan Mantri Swasthya Suraksha Yojna (PMSSY).
The main aim of setting up these new AIIMS was correcting of regional imbalances in quality tertiary level healthcare in the country and attaining self sufficiency in graduate and postgraduate medical education.
AIIMS Jodhpur is situated at outskirts of city of Jodhpur. The foundation stone of AIIMS Jodhpur was laid on 31 January 2004, by the then Finance Minister Jaswant Singh. From 2013 onwards, AIIMS started admitting 100 MBBS and 60 B.Sc Nursing (Hons.) students.
Outdoor patient (OPD) services began at the AIIMS Jodhpur from July 27, 2013. AIIMS Jodhpur started its MD courses in Anatomy, Biochemistry, Physiology and Community Medicine and Family Medicine departments from 2016.
College of Nursing
The College of Nursing, AIIMS, Jodhpur was established to train and educate nursing students so that they can make a significant contribution in all branches of nursing. The College became a benchmark in field of Nursing Education in India. The first batch of B.Sc (Hons) Nursing started on 2nd September, 2013 with an intake of 60 students. For more on nursing, click here
All India Institute Of Medical Sciences (AIIMS),
Basni Industrial Area Phase-2, Jodhpur-342005, Rajasthan
Tel. 0291-2740742 Hospital, Tel. 0291-2740741 Institution

domain: medical


text:
What do you know about the beautiful Czech Republic? It really is okay to grow older in place of earlier days. Young girls simply do not health care how vibrant you happen to be as long as you make the perfect husband/boyfriend. It is okay not to always be date czech women best, too.
The Czech females are known for their particular beauty. They will make a male fall in love with all of them in no time. But there is something that is disturbing many men coming from across the globe: exactly what are the features with the Czech girls? Do they have what it takes to get married to a Czech mail order wives? Can it be really feasible to find the right partner to get these pretty Czech ladies?
The Czech Republic ladies these are known as “Czech http://moscowbride.bravesites.com/ Mail Purchase Wives” due to their cultural record: they come from Czech countries (known as the Czech Republic) and their culture is noted with a traditional Slavic presence. The men who want to get married to these stunning women need to be aware that the standard of living in the Czech Republic is very low. The men are expected to be handsome, industrious, and healthier because it is a really poor nation, economically speaking.
The men who want to marry Czech mail buy wives need to learn the culture, and the way of life, in the Czech Republic. These kinds of ladies may not be offended easily, so they will not take care of any kind of unruly husband who tones her. If the guy is kind and well intentioned towards her, she will possibly be around to address his requirements, wants, and desires. She is going to also be now there to listen to him and offer her heart, and mind, in cases where he has got lost that since having been a young man. She will end up being there to laugh at his jokes and teach him about the fundamental principles of civilized habits, for example: can not talk undesirable about your superiors, and don’t deal with women like you’re less-than or equal to these people.
The fact that the ladies are known for being gorgeous and amazing makes it easier for the purpose of online dating websites to sell the Czech all mail order wives’ contracts to the people who are interested in finding a lifetime partner. If you want for more information about these girls, and you prefer to meet them, all you have to carry out is to go through a review of community online dating services. There are many sites for men and women who want to date overseas brides, and if you want in enabling your publish of this sort of relationship, then you definitely should take you a chance to examine review. You can find out the place that the best spot to meet these types of women is usually and how to methodology them in the greatest way.
A large number of people may think that it is weird for non-Western men to get involved in online dating sites services to find Czech brides. However , it is actually a really common factor, which is why there is no reason to never try it out, possibly. If you want to fulfill a new partner, who is lovely and delightful, who will absolutely adore you just as much as you love her, and that will be right for you, therefore maybe it has the time for you to meet local Czech brides. You just need to to read on the options, and see the type of relationship you are looking for.

domain: sociology


text:
Fertilizers help plants grow. They replace nutrients taken by the plants or washed away by rain. There are two types of fertilizer. They are synthetic and organic. Synthetics contain chemically altered products. Organic fertilizers are from natural resources. Organics contain plant, animal or mineral ingredients. Furthermore, organics have not been chemically altered.
What does N P K mean?
Plants require three major nutrients for survival. They are nitrogen, potassium and phosphorus. Therefore, they are referred to as macro elements.
N – Nitrogen is needed for growth. This includes roots, stems and leaves. It give plants their green color. Consequently, lack of nitrogen causes yellow or pale green leaves. In contrast, too much will kill a plant.
P – Phosphorus promotes strong, healthy root growth. It helps form fruits and flowers. Too little causes stunted growth. Hence, poor fruit and flower development.
K – Potassium or potash regulates the plant’s water retention. It also provides protection against frost and drought. Above all, potassium makes a plant more hearty and disease resistant. Too little causes weak stems. Leaves appear yellowish. Also, edges of leaves may appear burned.
What is balanced, complete or incomplete fertilizer?
A balanced fertilizer contains equal parts of each element. For example, 10-10-10.
A complete fertilizer contains all of the major elements. For example, 10-6-4.
While an incomplete fertilizer is missing one or two of the elements. For example, 0-20-0.
What is a slow release fertilizer?
Slow release fertilizers release nutrients over an extended period of time. Therefore, they don’t have to be applied as often. They are safer for the environment because there is less chance of leaching into the ground water.
Why is proper fertilization important?
There are many causes for unhealthy plants or lawns. The only way fertilizer can help is if lack of nutrients is the cause. Therefore, don’t apply fertilizer to a stressed plant until you know what the problem is. Is it too much or too little water? Is it too shady? Too sunny? Could it be the soil? Test your soil. Look for signs of disease or insects.
Plants that are growing in the wrong location will not respond to fertilizer. Too much will only compound the problem.

domain: agronomy


text:
Rostomaant Marani, established by the Rostomashvili family, can trace its history back to 1770 when the monk Rostom Orbeliani first cultivated a grape harvest. The dynasty has passed through six generations of Rostomashvili’s and is now run by Zurab and Irakli Rostomashvili. The logo is a portrait of Nikoloz Rostomashvili (1887-1967), who was the last owner of the company when it was at a prior location in Akhmeta.
Village Telavi, Telavi Municipality, Region Kakheti, Georgia
Rostomaant Marani owns two vineyards on either side of the Alazani River which are used for 70% Rkatsiteli, and 30% Saperavi grapes. Both have been harvested for over 35 years, and experience significant sun exposure.
One vineyard is in the Napareuli microzone, in the village of Saniore. This land hosts two rows of Saperavi grapes, and six rows of Rkatsiteli, yielding approximately 100 kilos of grapes per row. The second is in the Tsinandali microzone, in the village Kurdgelari. Annual sunshine in this locale is stunning--over 2,300 hours--which is ideal for vine health. Located on the north side of the Tsiv-Gombori Mountains, this small vineyard has recently been expanded from its original 10 rows to include 7 more, increasing production to around 3,000 kilos per annum.
Not yet represented in the U.S. market.
Rostomaant Marani Labels


Fans' hopes for a Camilla Cabello and Shawn Mendes reunion are fading in smoke. At the beginning of this week, the 25-year-old Cabello got into the lenses of photographers with a new boyfriend, thereby confirming that everything is fine in her personal life.
Mendez, by the way, was also suspected of a new romance with his doctor, Jocelyne Miranda, who has been working with the singer for a long time. But rumors of a romantic relationship between them have not yet been confirmed. In favor of the fact that the 24-year-old musician is still alone, the circumstance that has just been revealed also speaks.
According to The Sun, Shawn Mendes is actively trying to find a replacement for the former. The young man signed up on the Raya dating app. The closed online community, launched in 2015, is known for not being able to get into it - participants must go through a rigorous vetting process, during which a special committee determines, among other things, the degree of popularity of the future candidate, as well as his financial capabilities.
If accepted, each new member pays a membership fee to make sure they join for the right reasons, the dating site says.
Interestingly, Camilla Cabello's new boyfriend is also related to a similar dating app. According to TMZ, Austin Kevitch is the founder of the Lox Club, a private dating club. We would not be surprised if it was in this community that young people met each other.
Photos by Rolling Stone/Ruven Afanador


text:
Each week at Sunday mass,
Then afterwards he and I
walked the streets around Belfast.
Taking back the envelopes
from people who couldn’t go,
was something that Dad and I did
in the hail rain or snow .
My Da was a member of
The men’s confraternity,
Helping in his own way
All the parish community.
He was a silent witness and of him I was so proud,
Jesus knew my Father because he stood out in the crowd.
Many respected my Dad
for he lived what he believed
Like the hymn Faith of out fathers
I know I have received.
peace is a universal theme,
drive for peace,
vouch for peace today.
I wrote ,My father's Faith and was surprised and happy to see my poem on your website ,
My poems are like my little children who I send out into the world . Many of them grow up and return to me to show me the achievements they made . I am so proud of them all .
By Paul McCann
In some individuals this drug can create [url=http://albendazole.webcam/]buy albendazole[/url] raised sun sensitivity, leading to bad sunburns. These problems might be a contraindication or your [url=http://cheaplevaquin.science/]levaquin 500 mg tablets[/url] physician will have to adjust the dosage of Zoloft. Baclofen [url=http://avodart.science/]avodart online[/url] is suggested for alleviating muscle tightness and pain in clients with multiple sclerosis. There are other drugs propranolol is likely to connect with, so ensure your doctor learns about any sort of supplements or vitamins [url=http://buylevaquin.webcam/]buy levaquin[/url] you are currently taking. A client with well-known irritation [url=http://generic-elimite.nu/]where can i buy elimite[/url] to pain killers as well as penicillin, when treated with Cozaar, was withdrawn from research due to swelling of the lips and also eyelids as well as face rash, stated as angioedema, which went back to regular 5 days after therapy was ceased. The dose of Baclofen [url=http://ventolin.science/]more about the author[/url] relies on the signs being addressed and is generally higher than the preliminary one advised. Tamiflu is an antiviral prescribed medication that is made use of for the [url=http://cheapretina.party/]retin-a[/url] therapy of type A and B influenza.
Irbesartan is slightly soluble in liquor and also methylene chloride as well as practically [url=http://cephalexin.webcam/]cephalexin[/url] insoluble in water. Your [url=http://propecia.webcam/]buy propecia generic[/url] doctor might sometimes alter your dose to see to it you get the very best results. Calcium stations blockers lower blood pressure by disturbing the circulation of calcium right into both the [url=http://generic-atenolol.se/]atenolol[/url] heart and capillary. Do not [url=http://diflucanwithoutprescription.nu/]diflucan[/url] take Combivir with other medicine which contains zidovudine, emtricitabine, or lamivudine. It's additionally recommended for clients with damaged immune system (particularly if this has been induced by AIDS or HIV, bone marrow transplant, autoimmune illness or [url=http://prednisonenoprescription.nu/]prednisone[/url] cancer cells treatment).
Post a Comment


text:
This Feeling TV was guaranteed to be a huge event with Miles Kane announced as a headliner and it definitely lived up to expectations. Before doors had even opened the queue to get in stretched down the street, as fans poured to Nambucca to catch the three acts playing that night.
First up was alternative punk band Hands Off Gretel. Their funky but fierce performance had audience members mesmerised as they combined pop melodies with vicious vocals. Lead singer, Lauren Tate, was a force to be reckoned as she delivered a standout, riotous set, the perfect start to the night.
Indie-pop band Only The Poets were second on, with a younger audience making their way to the front to enjoy every single second of their favourite band. Right from the off fans could be seen dancing and singing along, as lead singer Tommy, grooved his way around the stage, only further encouraging the crowd and bringing a great atmosphere and morale to the room.
Miles Kane was the headliner of the night, bringing an acoustic performance to the room, as he treated fans to a new song, accompanied only by the acoustic guitar he was playing and a piano. Chants of Miles echoed around the room, with fans overjoyed at the intimate experience they were being treated with. “Thank you, that was very enjoyable”, Miles told the room as his set came to a close, but due to the demand of the crowd he went on to perform Rearrange, before disappearing into the night, attendees left revelling in what an amazing night they had experienced.


text:
It’s been nearly two decades since Sylvester Stallone ruled the box office with films like “Rocky” and “First Blood.” With the release next weekend of “The Expendables” — a retro men-on-a-mission film with Stallone out front and center — Lionsgate is betting that there is still gas left in Sly’s tank.
He won’t be doing the heavy lifting by himself. Stallone has cleverly surrounded himself with a cavalcade of current stars like Mickey Rourke and Jason Statham — and '80s has-beens like Dolph Lundgren — who are past their expiration date, but still command goodwill among filmgoers.
In true gang’s-all-here fashion, even Bruce Willis and Gov. Arnold Schwarzenegger pop up in cameos.
“Ten years ago, they couldn’t have afforded these guys, but now it’s a different story,” Jeff Bock, a box-office analyst with Exhibitor Relations told TheWrap. “This is an action junkie's dream team, and that’s how they’re selling it — with just a list of names.”
“They’re taking their lemons and making them into lemonade,” a rival marketing executive told TheWrap.
“Expendables” does not just represent a second chance for Stallone’s crew, it also offers an opportunity for redemption for Lionsgate’s marketing team.
The movie’s opening comes at a bumpy time in Lionsgate’s history. The studio’s other summer entry, the Katherine Heigl and Ashton Kutcher action romance “Killers,” crashed and burned. It only managed a lowly $46 million domestically on a $75 million budget. Although panned by critics, it also was crippled by a decision to release it concurrently with the similarly themed “Knight and Day.”
“There was a canceling-out effect, you even had trailers for both movies playing before the same movies at the same theaters,” Phil Contrino, editor BoxOffice.com, told TheWrap. “It’s a generic romantic-comedy premise that’s not strong enough for two movies back-to-back. It’s not asteroids hitting the planet.”
The studio also failed to translate rabid fanboy enthusiasm into a huge opening for its comic-book satire “Kick-Ass.” That movie grossed $96 million worldwide on a $30 million budget, but according to several marketing executives and analysts, Lionsgate’s schizophrenic campaign alienated fans. By playing down the movie's violence in trailers, Lionsgate failed to convey “Kick-Ass'” Tarantino-esque appeal.
“Lionsgate know how to sell ‘Saw’ and Tyler Perry movies,” Devin Faraci, editor of the film blog Chud, told TheWrap. “They messed up with ‘Kick-Ass’ because they made it look like ‘Mystery Men’ — and nobody liked ‘Mystery Men.’”
Lionsgate declined to comment for this article.
So far the approach on “The Expendables” seems to be working. The fanboy community has been worked into a lather through clever videos and digital promotions that revel in the macho cast. This week, for example, the studio released a witty faux interview with Stallone, which closes with the latter-day action hero blowing up the set with a bazooka.
Taking a page from Paramount's highly successful viral campaign for "Paranormal Activity," it's also prominently displayed a link so fans can post and share the video on Twitter and Facebook.
“This excites a certain kind of audience, and it's a choir that’s beyond excited, because they’re really preached to it,” Faraci said. “They’ve pre-sold that audience — but will anyone else want to pay money to see a DTV movie writ large?”
Indeed, Lionsgate seems hell-bent on playing to its base. For instance, don’t look for any distracting love interest, or even a butt-kicking female co-star to help attract women. It may not be a bad thing.
“You don’t want to be so focused on expanding the base that you water down the key asset of the movie, “ Vinny Bruzzese, president of the motion picture division for the research firm OTX, told TheWrap. “This is a cast that got put together so you could watch them kick some ass. It’s smart to play to that strength and not sacrifice that male audience for a single female dollar.”
In perhaps the starkest case of counter-programing seen at the box office this year, the movie is going up against Julia Roberts’ female empowerment romance “Eat, Pray, Love.” "Expendables" should open slightly behind that film with about $19 million for the weekend, according to BoxOffice.com’s Contrino.
“The only question is if there is a sense is a sense of urgency,” Bruzzese said. “There’s a strong interest level, but the deciding factor is whether those people are going to say ‘I’m seeing this movie.”
Even if the film is a hit, Lionsgate won’t see much of an impact on its stock price. The beleaguered studio has spent the past few months fighting off a hostile takeover from billionaire Carl Icahn. The renegade investor made hay out of “Killers'” poor showing by trying to get stakeholders to sell their shares, and even a hit with “Expendables” isn’t likely to change any minds.
“It’s not going to make a difference, because it’s all about the corporate actions right now,” David Joyce, an analyst with the investment firm Miller Tabak + Co., told TheWrap.

domain: entertainment


text:
He's a werewolf
She's his mate
Her family would kill to keep them apart.
A wolf in Cooper Landry's position doesn't really do the dating scene—there's simply no time when he's leading the SWAT team practically every day of the week. But when he meets beautiful Everly Danu during a bank robbery, he's sure she's The One for him. The problem: she has no idea what Cooper really is...until his secret is exposed and she discovers the man she thought she knew is monster in disguise.
SWAT (Special Wolf Alpha Team) Series
Hungry Like the Wolf, Book 1
Wolf Trouble, Book 2
In the Company of Wolves, Book 3
To Love a Wolf , Book 4
"SWAT is hot hot HOT!"—Kerrelyn Sparks, New York Times bestselling author
- Kindle Book
- OverDrive Read 0.9 MB
- Adobe PDF eBook 5 MB
- Adobe EPUB eBook 879.8 KB
Paige Tyler (Author)
Paige Tyler is a New York Times and USA Today bestselling author of sexy, romantic fiction. Paige writes books about hunky alpha males and the kick-butt heroines they fall in love with. She lives with her very own military hero (a.k.a. her husband...

domain: library


The fosera SCANDLE system has been carefully developed according to German engineering standards. The SCANDLE was designed to meet one’s everyday needs and can be used for several years.
To ensure maximum power output and reliability, the SCANDLE uses powerful rechargeable Lithium Ion batteries. The solar module uses crystalline cell technology in a durable glass and aluminum based encapsulation to increase efficiency under various weather conditions.
The lamp is manufactured with the highest efficiency, long lasting LEDs with brightness comparable to that of 15 – 35 Watt incandescent light bulbs, while consuming only about 4% of the power.
The innovative Plug & Play system allows users to install the parts on their own. The fosera SCANDLE is the first system of its kind that can grow with the demand of the user, as the unique modular design of the system allows users to connect several PSHS systems in parallel.
|fosera SCANDLE 200|
|System voltage||3.25 V|
|System capacity||1.4 Ah|
|Module peak power||1.3 W|
|Module current (IMPP)||295 mA|
|Illuminance (bright mode)||150 lm|
|Daily operation hours (bright mode)*||2.5 h|
|Daily operation hours (dimmed mode)**||35 h|
|Full battery runtime (bright mode)||3.5 h|
|Full battery runtime (dimmed mode)||50 h|
|Possible loads||phone charging, Radio, fairy light,
fans and many more
*Operation hours at an average irradiation of 5 kWh/m²/day
**Dimmed mode is 8 lm; sufficient for low light use. Operation hours after full battery charge


text:
Don’t let the case of Paul Valletta, one of the most prominent faces of Rhode Island’s firefighter unions slip by without reading some of the details. Here are some that WJAR’s Parker Gavigan has reported:
[Lt. Scott] Bergantino told police that while other firefighters were participating in the “Fill the Boot” campaign raising money for the Muscular Dystrophy Association, he and [Deputy Chief] Valletta got into a heated argument about overtime.
Valletta is also the Cranston fire union president and a state lobbyist for the Rhode Island State Association of Firefighters. Their argument turned physical, said Bergantino, who told detectives Valletta pushed him up against a chalkboard, punched him in the head two times and then threw him over a recliner and onto the floor.
Sleep well, Cranstonites, knowing that this is who responds to your emergencies, and sleep well, Rhode Islanders, knowing these are the state’s insiders, for whose benefit much of our public policy is designed. Note that the fight started on the subject of overtime, one key ingredient (other than pensions) making firefighting such a lucrative career in Rhode Island. A quick review of Cranston budgets suggests that the department regularly overspends its overtime budget by around $1.2 million (about 34%).
According to Gavigan, it appears that police have some sort of recording of the incident, and Valletta is currently suspended with pay. Anybody want to bet against my expectation that Valletta will find himself in a graceful retirement and Bergantino will get a nice bit of time off?

domain: news


Sur paperjam.lu, nous utilisons des cookies pour mémoriser vos préférences, gérer la publicité, vous proposer des contenus toujours plus pertinents selon vos centres d’intérêt et améliorer sans cesse votre expérience utilisateur. En poursuivant votre navigation, vous acceptez l’utilisation de ces cookies.Fermer
Itenos, Bonn, and vyzVoice, Luxembourg, have entered into a partnership that will bring state-of-the-art solutions in data centre infrastructure management (DCIM) and deliver new innovative services to data centre customers.
Optimising operations, serving customers
The continued growth in the demand for data facilities and services has resulted in rapid expansion of the number of data centres globally.
With data centres already consuming up to 3% of the global electricity supply and leaving a carbon foot print equivalent to that of the airline industry, effective energy management and increased use of renewables are key to supporting this growth, while also ensuring sustainability goals and regulatory compliance standards are achieved.
Analysis by the Borderstep Institute for Innovation and Sustainability indicates that, in Germany alone, data centres consumed a total of 12.4 billion kWh of electricity in 2016 – and consumption expected to rise to 15 billion kWh by 2020.
Demands for storage, availability and security have also expanded, along with an increasingly stringent regulatory environment for data privacy.
Combined with the potential for creation of new services, effective DCIM has become a key differentiator for data centre operators.
Optimising operations, empowering customers
Itenos currently operates four data centres in Germany. The vyzVoice solution for DCIM is currently being deployed at all sites.
vyzVoice is being integrated into the existing infrastructure and provides data analytics across all levels of Itenos operations, from the physical infrastructure management through to optimisation of energy consumption. The solution includes power and capacity planning capabilities and asset management of all hardware. Customised notification procedures and automation of processes ensure operational efficiency and optimal uptime.
With this partnership, Itenos will deliver vyzVoice solutions to its data centre customers, thereby providing advanced, integrated services across its entire ecosystem. “Working together”, says vyzVoice CEO, Robert Spicer, “Itenos and vyzVoice have succeeded in advancing digital transformation, for data centres and their customers.”
With customer-level, real-time, insights for energy efficiency and capacity improvement, Itenos is leading the data centre industry by empowering its customers with the tools to make data-driven decisions.
“In vyzVoice, we have found a competent and reliable partner,” says Bernd Mitternacht, Itenos CEO. “In a business environment where flexibility is an immense challenge, vyzVoice has demonstrated from the beginning what customer-oriented solutions really mean.”
Le contenu de ce communiqué de presse est de la seule responsabilité de son auteur: "vyzVoice"


text:
Age Restriction: Under 14 accompanied by an adult 18+, Strictly no under 14’s on the Standing Floor
Bio: Diana Ross
Diana Ross returns to the U.K. with an electrifying tour of music, memories and career magic. These arena concert performances will be a thrilling evening of great fun, music, glamour and love. With all of her spectacular costume changes.
Diana Ross is set to perform in the most anticipated “Legend" spot at The 50th Anniversary of Glastonbury on June 28 with her ‘Diamond Diana Top of the World Tour.’
Diana Ross is an icon and international treasure with a lifetime of music including, “Ain’t No Mountain High Enough,” “I’m Coming Out,” “Stop! In The Name of Love,” “Upside Down,” “I’m Still Waiting,” “Chain Reaction” and many many more.
“I look forward to coming to the UK to be with all my fans, friends and family, it is an honor and a privilege to sing and dream, every concert is a blessing and a gift, I am filled with excitement and huge appreciation. Dreams do come true,” says Diana Ross.
DIAMOND DIANA TOP OF THE WORLD TOUR 2020
Jun 30 Leeds First Direct Arena July 1 Liverpool M&S Bank Arena July 3 Manchester Arena July 4 Glasgow The SSE Hydro July 6 Arena Birmingham July 8 London The O2
A new ticketing feature that allows customers to reserve tickets for friends, so that everyone can attend the event together.
Find tickets – When purchasing tickets, select a quantity for yourself and then a quantity to reserve for friends. (For events with assigned seating,our system will return a block of seats so you can all sit together.)
Purchase your tickets and create the reservation – You only have to pay for your tickets. Reserving tickets is FREE.
Invite your friends – After your purchase, you can easily invite your friends via Facebook or email. They'll have a limited amount of time to purchase the reserved tickets! We'll manage the rest and send you status updates when friends accept or decline your invitations.
Experience the show together – That's it, have fun!
With AXS InviteTM, coordinating with friends has never been easier.


The deadline for implementation of Transportation Worker Identification Credentials (or TWIC cards) has officially passed. Now, the Coast Guard is working to develop requirements for the card readers that will be deployed at ports across the country. The Coast Guard held a public meeting yesterday to discuss its thoughts on this and gain ideas from those that will be affected. Commander David Murk is the chief of the Cargo and Facilities Division for Coast Guard Headquarters. He’s also responsible for the Coast Guard’s implementation of the TWIC program.


Every year influencers always come up with their predictions for the next year. What will the hottest new trend be? What will the game changing technology be? What technology will all other innovations be based upon? Normally there isn’t a single technology that I believe will be the center of all things, but this year cognitive computing is taking the gamut.
With everything from IBM Watson in Cancer research to Amazon’s Echo in home automation, consumers and technology are working together to get things done and learn from one another. This collaboration will bring along things that humans alone would never be able to accomplish. We’ve been very focused on real time marketing and results in the past, but now with all the new quickly and efficiently analyzed data and learned knowledge, we’ll be able to deploy “right – time” marketing tactics.
This year and the years to come will have much to do how humans and technology can learn from one another. Technology will learn from us, our patterns, habits and what actions reap the best results. It will be able to present the results to us Humans so we can in turn use this data to create a personalized consumer experience. Collaboration is key this year, and changes will be seen all over the industry, especially in Security, to be able to support this collaboration and allow us to continue to grow and learn together.


text:
Welcome to our Stamp of the Month Blog Hop! This is simple to do--just click on the link at the of the post on each blog to move to the next one. Here we go! You probably just arrived from Brea Montgomery's Blog after looking at my artwork you will find the link for the next blog.
This was my first attempt and I will post some updates to this a little later today that I liked even better....
Supplies used are all Close To My Heart: Level II Jingle paper pack; D1376 Holiday Warmth (Stamp of the Month); Tulip CS; Gold EP; Gold Ribbon (retired). I also used glitter (retired) on the Christmas bulbs. But, I finally decided on this:
Now, "hop" on over to Tanya David's Blog - to see her lovely work! If you get lost on the Hop, or find links that aren't working, see list below. Thanks for stopping by!

domain: hobby


| 2017 Pop Album:
Annette - All Our Achilles Heels
|Annette news stories|
Annette B releases EP 'Beautiful Mama'
New urban gospel acts showcased on Malachi Records' various artists project
Starring A Star
Integrity Music release 'Christmas Starr' musical.
Tony Cummings reports on the life and music of recently deceased singer, songwriter and group leader GABRIEL S HARDEMAN [13.07.12]The Davis Sisters: The Philadelphia sisters dubbed Queens of the Gospel Highway
Tony Cummings remembers one of gospel music's greatest acts THE DAVIS SISTERS [19.10.11]The Spirit Of Rock And Soul: Numbers 981 to 990
The latest part of the ongoing series chronicling, in no particular order, the greatest 1001 recordings made by Christian artists [24.08.17]Larry Norman: The Growth Of The Christian Music Industry
In the first part of a personal history, LARRY NORMAN, Christian music pioneer, charts the rise of Christian music from its roots and, based on his experiences, gives his own perspective on the collision between creativity and commerce. [11.10.06]
|Annette contact/database details|
Artist ID: 31519
Country: United States


text: I have reached a point in my fourth volume in which the Young Guardians’ five new recruits are first introduced. As I describe them, their abilities, and traits, I have decided that one of my future works will involve a trilogy focusing on them. They are an interesting group of characters from different lifestyles and I have planned the first volume of this trilogy out. As I go deeper into the fourth volume, I look forward to seeing how they will be accepted into the Young Guardians in preparations for the battles ahead. I will keep you posted on any further developments.


text:
HOPES that America is bouncing back to a period of strong recovery were dashed yesterday, as a final estimate showed the economy expanding by just 1.8 per cent in the year to the end of March 2013.
GDP had previously been reported to have grown by 2.4 per cent during the 12 month period, with many economists expecting the figure to be confirmed by the latest reading.
The number suggests the US Federal Reserve may hold back its plans to slow down purchases of assets, known as quantitative easing (QE) and designed to stimulate the economy. It is currently spending $85bn each month on QE.
Yet some analysts believe the US economy remains relatively strong. “Growth may have been revised down slightly, but the US is demonstrating the type of economic improvement most of the western world would be jealous of, despite sequestration and tax rises,” said Nancy Curtin of Close Brothers Asset Management. “The private sector is still picking up speed, with first quarter consumption growth up over three per cent, compensating for shrinking government spending,” she added.
Consumer spending, which accounts for more than two-thirds of US economic activity, increased at a 2.6 per cent pace rather than 3.4 per cent. The revision largely reflected weak outlays on health care services.
Consumer spending grew at a 1.8 per cent rate in the fourth quarter of last year.
And exports, previously reported to have grown, actually contracted at a 1.1 per cent pace in the first quarter, cutting 0.15 percentage point from GDP growth. That likely reflects a slowdown in the global economy.
Business spending barely grew, with investment on nonresidential structures declining more sharply than previously reported.
The drop in spending on non-residential structures was the first in two years, the data showed.

domain: economics


Will Smith has announced his first project after Oscar’s slap incident. It’s been six months after the publicized assault of actor Will Smith on comedian Chris Brown on stage at the 94th Academy Awards.
Recent reports show that the actor is being sought to star in filmmaker sharpen Obaid-Chinoy’s feature directorial debut, Brilliance.
Apparently, Smith will also be producing the project with director Akiva Goldsman as his passion project.
Goldsman is a long-time collaborator of Will Smith, who’s also directed movies such as I, Robot, I Am Legend, and Hancock, all of them Smith-starring films.
Brillance is a sci-fi thriller. However, Smith’s participation as the protagonist has yet to be confirmed, but still very possible. He’d play Nick Cooper, the hero of Marcus Sakey’s novel of the same name.
The three books of the Brilliance trilogy include: Brilliance (2013), A Better World (2014), Written in Fire (2016). Many of these are inspired by autism as a plot point.
The Brilliance trilogy is a series of novels where neurodiverse people are demonized by society.
They are called “twists”, or “abnorms”. These non-neurotypical people are labeled “Brilliants”, and are tracked by the government.
The films are set to analyze the disruption in society. Smith is set to play agent Nick Cooper, a gas man working with the Department of Analysis and Response, whose job is to eliminate Brilliants who pose a danger to society.
However, Cooper is a Brilliant himself, with the ability to read body language before the person in question takes the actions.
Smith is also set to participate in an AppleTV+ project shot called Emancipation.
The project was shot before the Oscars, where he plays a runaway enslaved man in Louisiana, in Antoine Fuqua’s true-story thriller.
After the Oscars, Will Smith’s popularity rating with the public and other artists decreased, as per Q Scores, the popular metric in show business to better assess a performer’s approval rating.
It was in the 94th Oscar’s ceremony where Chris Rock made an ill-mannered joke about Jada Pinkett Smith’s alopecia, and the actor hit Rock across the face.
The Oscar’s slap caused Smith to practically vanish from the public eye for a couple of months, until he opened up about his moment of self-reflection after the experience.
Smith has since apologized to Rock, who has declined further comments about the situation.
For some, this might be Will Smith’s career comeback, but no one can ascertain that fact until more details of the project have been revealed.


text:
PoolThis is one of few homes in this area that has this feature.
Big comfortable well furnished bedroom in house 1 mile from downtown englewood and 2 blocks to shops, NYC Buses, houses of worship.
You must also acknowledge
Pet(s) live on property
Security Deposit - if you damage the home, you may be charged up to $279
This place is homely and cozy. Perfect for friends and small group of people. No central heating, but it was okay as there is a room heater. This place is located in New Jersey so if you are planning to visit New York, then you have to take cab, and its very expensive in here. If…
Easygoing , quiet,
Languages: Deutsch, English, Polski
Response rate: 88%
Response time: within an hour
Always communicate through AirbnbTo protect your payment, never transfer money or communicate outside of the Airbnb website or app.

domain: travel


text:
NEW YORK (Reuters) - IEX Group, the newest U.S. equities exchange operator, is actively courting companies to list their stocks on its exchange, according to a letter sent to potential listing clients that was obtained by Reuters.
IEX plans to begin listing companies in 2017, and is offering “significant incentives” to its initial founding issuers, according to the letter, which did not go into more detail.
IEX declined to comment.
The New York-based company, which was featured in Michael Lewis's book "Flash Boys: A Wall Street Revolt," will challenge Intercontinental Exchange Inc's ICE.N New York Stock Exchange and Nasdaq Inc NDAQ.O for corporate listings.
“Flash Boys” followed the exchange’s founders as they built what they saw as a more investor-focused market that included a “speed bump” to slow trades by a fraction of a second to help level the playing field for fast and slow traders alike.
The book caused a heated debate on Wall Street after Lewis said the market was rigged by profit-hungry exchanges in favor of high-frequency trading firms.
IEX launched The Investors Exchange on Aug. 19 after operating an off-exchange trading platform for more than 2-1/2 years, and is hoping its unique model will attract business.
“Our exchange is designed to protect long-term fundamental investors from predatory trading strategies that increase costs for your shareholders and can cause unnecessary volatility in your stock,” IEX said in the letter.
NYSE and Nasdaq compete fiercely for corporate listings, from courting initial public offerings to luring companies already listed on their rival exchanges, both of which can be public relations windfalls.
Exchanges charge various listing fees based on factors such as the amount of shares a company lists.
Last quarter, ICE earned $105 million in revenue from corporate and other listings. NYSE lists more than 2,400 companies, according to its website. (www.nyse.com/list)
Nasdaq earned $68 million in revenue from listing services last quarter. The exchange lists around 3,100 companies, according to its website.
Company shares can trade on all 13 U.S. exchanges, regardless of where they are listed.
NYSE offers listing companies the chance to ring the opening or closing bell on the floor of the New York Stock Exchange, giving them exposure to the TV news channels that broadcast from the iconic location. Nasdaq puts listing companies’ names on an electronic billboard at its Times Square location in New York.
Bats Global Markets BATS.Z lists its own stock, but has focused on listing exchange-traded funds rather than companies.
Reporting by John McCrank; Editing by Paul Simao
Our Standards: The Thomson Reuters Trust Principles.


text:
As part of a broader plan to double its automobile axle output capacity in America, Japan's NTN Global, parent company of NTK Precision Axle Corporation, recently announced plans to build a manufacturing plant in Anderson, Indiana, in April of next year, Nikkei Asian Review reported. The multi-million dollar project is expected to add approximately 200 jobs.
The plant, which will be located next to one set to open next month, will be responsible for cutting and heat-treating iron used in the construction of drive shafts. The news source indicated that this move may stem from the urges U.S. President Donald Trump has given to automakers to increase domestic production and employ more American workers.
Both Ford Motor and Toyota have recently announced plans to invest in U.S.-based facilities in the near future.
Fox59 reported that NTK currently employs over 4,500 people in the U.S., almost half of which are based in Indiana.
"Our parts make their way to more than 15 different automakers, which translates to one fourth of the cars and light trucks on the open road riding on our products," NTK President Tadao Okamura told the source. "We are excited for Anderson to become part of our company and we look forward to a long and prosperous relationship with both the city and the state."


text:
Recent numbers of illicit drug overdoses are suggesting that measures in place during the COVID-19 pandemic are taking an increasingly lethal toll on British Columbia’s other public health emergency.
In May the province saw 170 deaths linked to drug overdoses – the highest monthly total since the opioid crisis was declared a public health emergency in April 2016, according to numbers announced on June 11 by the BC Coroners Service. At least three of these deaths were to members of Nuu-chah-nulth nations, according to information gathered by the Ha-Shilth-Sa.
This breaks a decline in the number of fatal overdoses that was seen before the novel coronavirus commanded the attention of health authorities in mid March. B.C.’s illicit drug death total dropped from 1,546 in 2018 to 979 last year.
But a rise became evident in March, when B.C.’s fatality total surpassed 100 for the first time in a year. The increase has continued through the COVID-19 pandemic, and May’s fatal overdose tally exceeds the total number of people in B.C. who have died with COVID-19 since the respiratory disease began spreading early this year.
“[W]ith the immense pressure of two public health emergencies, so many unprecedented factors are bearing down swiftly on us all at once,” said Mental Health and Addictions Minister Judy Darcy in a media statement. “Borders are closed and the usual illegal supply chains are disrupted, leading to drugs that are more toxic than ever.”
Toxicology analysis shows that 119 of the 170 overdose deaths in May were linked to Fentanyl. In April and May, 19 per cent of those who died had “extreme concentrations” of Fentanyl, reports the Coroners Service, compared to the nine per cent rate found in fatal overdoses over the first three months of this year.
Despite the rise in fatalities, ambulance calls for overdoses have not increased during the pandemic. BC Emergency Health Services reports a stable rate of 20 overdose calls per 100,000 residents in the province this year, less than what paramedics saw in 2019, when the rate spiked at 30 per 100,000 residents in March of last year.
Kevin Hollett, communications lead for the B.C. Centre on Substance Use, attributes the recent rise in fatal overdoses to street drugs becoming more dangerous during the pandemic, rather than people using more. The usual drug supply chains have been disrupted, resulting in B.C. dealers selling more hazardous combinations of narcotics.
“It already was incredibly toxic and dangerous, but it’s getting worse,” said Hollett. “We know that the illegal drug supply is a global supply chain, so production, trafficking, all of those things getting disrupted through closed borders and other preventative measures…that’s definitely a contributing factor.”
“The drug supply is unpredictable and highly toxic, and has led to a sustained increase in fatal and non-fatal overdoses from smoking and injection in recent months,” said Dr. Jane Buxton, harm reduction lead for the BC Centre for Disease Control in a media release from the province. “COVID-19 has added challenges and people may be feeling more isolated and anxious, but it’s important to continue to buddy up, or access local overdose prevention and supervised consumption services during this time.”
The Coroners Service has reported no deaths in B.C.’s 32 safe injection sites, which are supervised to protect users from fatality. These locations were declared essential services during the COVID-19 pandemic, although sanitizing and social distancing measures have been introduced to mitigate the spread of the virus.
Port Alberni’s safe injection site on 3rd Avenue has continued to see consistent use, although fewer clients than usual were visiting the site this spring, according to Wes Hewitt, executive director of the Port Alberni Shelter Society, who spoke with Ha-Shilth-Sa in late May.
In Victoria, The Harbour on Pandora Avenue, which is Vancouver Island’s busiest safe injection site, shifted its services to a supervised mobile program to ensure COVID-19 social distancing measures are followed.
“Starting April 3, the onsite overdose prevention site will shift to a mobile service, providing harm reduction and overdose prevention in the community,” reads an Island Health announcement on The Harbour’s services during the pandemic. “The Harbour building will remain open, serving as a triage site to support people without respiratory symptoms who are homeless and underserved. People with respiratory symptoms will be assessed and await their results at a location that supports self-isolation.”
Hollett said that the pandemic has not encouraged people to use drugs at safe injection sites.
“Like anyone else, people who use drugs are hearing the public health messaging about physcial distancing, following that advice and not wanting to go to spaces indoors where the risk of exposure to COVID-19 is going to be greater,” he said. “In Vancouver, access to the supervised consumption sites there have gone down 45 per cent over the last couple of months.”
Provincial health authorities have also focused on methods to separate people from the increasingly toxic drug supply. In May 1,686 prescriptions were made for hydromorphone, a synthetic opiate, showing a 149 per cent increase compared to what was dispensed in March. No overdose deaths have been linked to hydromorphone, according to the Ministry of Mental Health and Addictions.
But Hollett cautions that much more needs to be done to connect users with less harmful opiates.
“It’s estimated that there some 50,000 people with an opioid use disorder in B.C., and we’re talking about 1,700 or so who have had access to the safe supply measures,” he said. “Efforts to separate people from the toxic drug supply are what’s needed, but until we’re able to really scale that up and look at different options for how people can do that, this isn’t going to get any better.”


text:
This is why you have to love the Internet. Two stunt women have been hired to reenact the epic chicken vs Peter fight form Family Guy.
Via Gawker: “The deal was almost too good to be true: Pay professional stunt women/actresses Jessie Graff and Tree O’Toole just $2,800, and they will gladly pretend to beat the crap out of each other for a live-action parody of the “Epic Chicken Fight” from Family Guy.
And so the Internet dug deep into its parents’ couch cushions and pulled out almost $3,000 to make it so.
And so it was: A full-length, (almost) shot-for-shot reenactment of the classic battle scene between Peter Griffin and Ernie the Giant Chicken.”


text:
CT Sec. 14-233. Passing on right. The driver of a vehicle may overtake and pass upon the right of another vehicle only when conditions permit such movement in safety and under the following conditions: (1) When the vehicle overtaken is making or has signified the intention to make a left turn; (2) when lines of vehicles traveling in the same direction in adjoining traffic lanes have come to a stop or have reduced their speed; (3) upon a one-way street free from obstructions and of sufficient width for two or more lines of moving vehicles; (4) upon a limited access highway or parkway free from obstructions with three or more lanes provided for traffic in one direction. Such movement shall not be made by driving off the pavement or main-traveled portion of the highway except where lane designations, signs, signals or markings provide for such movement. Violation of any provision of this section shall be an infraction.
So, if a car is slowing or stopped in your lane and signalling for a left turn you can pass it on the right if you don't go off the road (pavement) or cross the white line onto the shoulder. The travel lane (yellow center line to right edge line) of most 2-lane roads is not wide enough to allow you to legally pass on the right. That is the situation at the flagpole.
Please compare CT election laws to GA, FL, etc and redirect your disdain at the ruling party in your beloved home state. Many aspects of the laws here in CT are much more restrictive and 'obstructionist'!
I love commentary on vaccines from people who only drag out the tried and true vaccines of yesteryear and not the numerous vaccines that have not been vetted in the regard that we don't REALLY know what consequences they have wrought over the long term. Do you have the facts and studies that show that autoimmune disorders are rampant? ADHD, autism, increase in depression, anxiety etc? All I know is that there are not sufficient studies to show that perhaps we have traded one thing for another. I'm not claiming to know if there IS a direct link, but could there be? So while you shame others by claiming they are "shirking" responsibility, maybe they are just critically thinking and waiting for those studies by the pharmaceutical companies to come out. Oh that's right, there won't be any, and the ones who have no obligation to you should a health concern, illness or even death occur, is not liable. They only stand to gain financially.
As for the religious exemption, there are some who believe all lives matter and that a few sacrificed lives that didn't choose to be killed, are now in a sense, continually mass produced "parts" for your "greater good". They never had a voice. So excuse some of us that value those souls and choose not to tamper with what God made and not make them a product to inject ourselves for our greater good.
In a generation of everything now, all actual science has flown out the window. I choose to wait to see what exactly the risks may be down the line.
it is only permissible, in the State of CT., to pass a motor vehicle on the right, when passed vehicle is making a left turn. This means, say on I-84, that if you pass a car, that is in the left lane, while travelling in the right lane, you are in violation. In the flagpole instance, if a crash occurs, you would be ticketed for failure to drive in the lane, because you crossed the white line. The line is there, in this instance, to designate travel lane/parking.
Scarlett Lewis was one of the parents who lost a child in the Sandy Hook School Shooting. Tragic doesn't begin to define it. In Jan 2015 Scarlett was one of 2 parents who sued our town and the BOE claiming that there was inadequate security at the school. In June 2016 They offered to settle for 5.5 million dollars!
In May2018 a judge dismissed the law suit. They appealed and in Oct 2019 they lost the appeal I have difficulty understanding her action to sue and at the same time promote 'choose love' It doesn't make sense. Where was the love for her town and neighbors that grieved with her, that supported her?

domain: law


The local stock market recovered some of the previous day’s losses and returned above the 7,000 level as investors found prices of heavyweights too low to resist.
The main index jumped 202.97 points or 2.98 percent to close at 7,019.92 with the Property and Banking sectors leading the advance although Mining and Oil stocks fell. Volume rose to 3.61 billion shares worth P9.61 billion as losers still beating gainers at 138 to 76 with 36 unchanged.
“Investors turned into bargain hunters with many looking ahead to the Federal Reserve’s next monetary policy decision later this week amid an ongoing war in Ukraine and soaring inflation,” said Regina Capital Development Corporation Managing Director Luis Limlingan.
He noted that, “Consensus expects the Fed to raise its target fed funds rate by a quarter-percentage point from zero at the end of its two-day meeting on Wed. The move was also influenced by the resumption of talks between Ukraine and Russia, sending commodities prices lower.”
“Meanwhile, at home, many shares neared oversold levels, signaling buying opportunities for those looking to come into the market,” Limlingan added.
Philstocks Financial Senior Supervisor for Research Japhet Tantiangco said “Bargain hunters took opportunities out of Monday’s steep decline which even got extended in Tuesday’s morning session.”
He added that, “The sustained decline in the country’s new COVID-19 infections with March 7 to 13’s additional cases amounting to 4,131, down by 35 percent week-on-week, gave market sentiment a boost.“
Credit belongs to : www.finance.yahoo.com


text:
Dec 11 - Dec 12
Hotels in Dollar Mountain start at $104 per night. Prices and availability subject to change. Additional terms may apply.
Dig out your winter woollies and prepare for some fast-moving fun at Dollar Mountain.
It is located in Sun Valley, which is roughly 95 miles (153 kilometers) from the state capital, Boise.
It'll be a short commute from the front door of your hotel to Dollar Mountain if you choose to stay at one of the 20 possibilities that are in the vicinity. The Sun Valley Resort, 1 mile (2 kilometers) away, is one place to start. You may also like to look into the Knob Hill Inn, 1 mile (2 kilometers) away, and the Best Western Plus Kentwood Lodge, 0.8 miles (1.3 kilometers) away.
More frugal guests might appreciate the International Village in Warm Springs by Alpine Lodging, 3 miles (5 kilometers) away.
If you're ever stressed from your travels, try not to let it get to you. You'll be well looked after when you spend some time at the Summit Condominiums in Elkhorn by Alpine Lodging, only 0.8 miles (1.3 kilometers) away.

domain: geography


text:
Payal Rohatgi suffered a wardrobe malfunction, the controversial girl from Bollywood was recently snapped at an award ceremony in an uncomfortable situation. Payal Rohatgi was seen in this awkward position at Radio Mirchi Music Awards 2012, held in Mumbai.
At the awards event held recently, actress Payal Rohatgi was seen sashaying down the red carpet in a backless gown. Payal Rohatgi was posing confidently in front of the cameras. Then all cameramen went into shock. The actress exposed a bit too much of her body, and the eyes behind the cameras made sure they clicked every bit of flesh that the actress gown had accidentally revealed. Whether the incident left Payal in embarrassment or not, we are yet to know!
Payal Rohatgi Oops Moment
Payal smiling for the camera.
1,507 total views, 3 views today


"Urban Heat" sounds like a cool name for a band. Unfortunately it's nothing like that. In fact, it's not exactly cool. The "urban heat island" refers to the fact that the average temperature of a large city is higher than the surrounding rural area. Sometimes, quite a bit higher.
This is because a lot of energy is used in cities, for transportation, heating, cooling, lighting, running computers, washing machines, coffee makers and all the other machines that make modern life possible. And once that energy has done its job, it turns into heat.
And that's even before taking into account some other things that make it worse, like the lack of cooling trees, and the presence of dark asphalt and rooftops that are very good at absorbing heat from the sun. So there is more than fresh air that makes it so "refreshing" to get out of the city and into the countryside.
Urban heat is hard on cars. On a nice sunny day cities can be warmer than the area around it by up to 27F (15C). And if you park your car in the sun, it can get hotter still.
This hapless Megane got stuck in a confluence of unfortunate circumstances: Its owner had parked it in the sun, on an asphalt lot, in an Italian town, right when a heat wave hit it in August 2015. Oh, and the car was dark blue. Outside, the temperature was in the high 30s centigrade (38C =100F), and since a car is a nice greenhouse under any circumstance, the temperature inside the car must have shot way up: high enough to cause the plastic parts on the car to melt.
This photo makes me wonder what the inside of the car must have looked like. I imagine the radio could have looked a bit like Dali's melted clock. In fact, any electronic part must have suffered immensely. And think of all the plastic parts from the carpeting to the wire insulation. So there's another reason to leave you car out of the city, at least on very warm and sunny days.
Here is a cool interactive graphic from the amazing folks at Climate Central, illustrating the urban heat effect for an urbs, that is to say a city, near you.
Go ahead: give it a spin. Then, next time you go downtown on a hot day, take the train or bus. It keeps one more heat engine off the streets, and spares your car the wear and tear of being in a furnace. Not to mention the risk of melting its electronics.
You may also like:
1. EV Battery seeks TLC for Long-Term Relationship, Hates Temperature Extremes both Cold and Heat
2. How you pay for car features that you don't need
3. How to Save Water (and Work) When Washing Your Car