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MEMBA have returned with a brand new single to close out the first month of 2020. Signed to ODESZA’s thriving Foreign Family Collective, the producer duo have seen an eventful 2019, especially with the release of their audiovisual project SAGA-II. Now they've unleash “Meet Freedom,” a song embodying the vibrations of being free.
When it comes to their sound, they describe it as “world influenced and unexpected. We don’t like doing the same thing twice, it’s all about combing things in a way that hasn’t been done.”
The two like to keep their fans on their toes. The song features their U.K.-based friend, Levus Alone. “We’ve been writing with him for a long time,” they explained in a joint statement. “We recorded these vocals about four years ago while he worked at a Bingo in Leeds. The instrumental came together a lot later. We don’t know what he’s up to these days. He’s been hard to reach, but he’s one of the most talented people we’ve worked with and he really does like to be left alone."
MEMBA have performed at festivals like Arise Music Festival, Resonance Music & Arts Festival, Coachella and Ultra Music Festival. Fans can expect a series of North American tour dates kicking off in the spring.
"The Best to Ever Do It": Legendary DJ Paul Johnson Has Died Following COVID-19 Battle
The pioneering Chicago DJ had been hospitalized and placed in intensive care after contracting the coronavirus last month.
DJ Snake Shows Chameleon Colors In Electrifying 2021 Tour Debut
Adapting to the surprise absence of Malaa, DJ Snake thawed the touring freeze with a fiery show in Chicago over the weekend of Lollapalooza 2021.
Alison Wonderland Teases Return of Whyte Fang Alias With Never-Before-Heard Song: Watch
Not much is known about Whyte Fang, but Alison Wonderland offered fans a glimpse into her plans for the revival of the decade-old alias.


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All dogs have the potential to develop genetic health problems, just as all people have the potential to inherit disease. Conditions that have been seen in the breed include hypothyroidism and deafness. Dogos may also be prone to glaucoma and laryngeal paralysis. And, like many large and giant breeds, the Dogo can develop hip dysplasia.
Hip dysplasia is a hereditary defect of the hip socket. It can be mild, causing little or no pain, or it can lead to severe lameness. Dogos with hip dysplasia may move slowly or avoid jumping. Depending on the severity of the condition, weight loss, medication, or surgery can help to relieve pain. Dogos that will be bred should have their hips x-rayed and graded by a veterinary orthopedic specialist at two years of age. Ask the breeder to show written evidence that a Dogo puppy’s parents have hips that have been rated as fair, good, or excellent by the Orthopedic Foundation for Animals (OFA).
Image: Ormando SLR – flickr
The Dogo is a fairly healthy breed, with a typical life span of between 9 and 12 years. Breed health concerns may include congenital deafness (can be unilateral or bilateral; in one or both ears), hip dysplasia, bloat/torsion (gastric dilatation and volvulus; GDV) and demodectic mange (demodex).
The primary genetic fault that “comes with” the breed, because it is a white-coated dog, is deafness. The Dogo Argentino Club of America monitors all litters whelped to DACA-registered parents. The percentage of deaf puppies is roughly 10% overall, which is the same percentage as that of the Dogo club in Germany. All Dogo Argentino puppies sold by DACA members should be accompanied by either a statement from the breeders’ veterinarian attesting to the fact that the puppy can hear, or a BAER (hearing) test report.
Careful breeders screen their breeding dogs for genetic disease and breed only the healthiest and best-looking specimens, but sometimes Mother Nature has other ideas. A puppy may develop one of these diseases despite good breeding practices.
Grooming NEXT page

domain: medical


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Explore 15+ million radio shows, DJ mixes, podcasts and tracks made by passionate creators.
15 Dec 2022
This upload features tracks from Man In The Moon, Monte La Rue and more.
A chilled, downtempo lounge collection that I've had hanging around for numerous months. A number of the selections have a "moon" theme, hence the ti...

domain: entertainment


Patrick Wiercioch is coming home.
The 26-year-old defenceman has signed a one-year contract with the Vancouver Canucks, bringing his professional hockey career full circle.
For Wiercioch, who was born in Burnaby but grew up in Maple Ridge, the signing is a dream come true.
“It’s unbelievable,” he said over the phone from Kelowna, where he’s been spending the summer with his wife and young son.
“It’s such a special thing, to be asked to play for the team you grew up watching as a child. I idolized so many of those players, so to be given the opportunity to follow them is very exciting.
“To play for a Canadian team as well, that’s truly special for me.”
Wiercioch should know. The NHL veteran previously logged four full seasons with the Ottawa Senators, helping the team make the first round of the playoffs in two of those years.
He spent last season with the Colorado Avalanche, which was also something of a homecoming for Wiercioch, who played for the University of Denver during his college days.
That meant he missed out on the Senators’ thrilling playoff run that saw them almost overtake the eventual Stanley Cup champions the Pittsburgh Penguins in the third round. But Wiercioch said he was nothing but excited for his former teammates.
“You know, I still have a lot of friends on that team who I keep in touch with, so to watch them make it so far, that made me very happy,” he said.
“That was a huge accomplishment, to end up being the only Canadian team left in the running. As I fan, that was great to see. I couldn’t help but be proud.”
The spirit of Canadian hockey is clearly strong in Wiercioch, especially when he talked about growing up in the Maple Ridge-Pitt Meadows area.
The local hockey community has been a breeding ground for plenty of talented players like Wiercioch, who said the influence and coaching he received during those years had an immeasurable impact on his career.
“There’s just so many wonderful people I had the privilege to work with and work under, who all influenced me and shaped me in so many ways,” Wiercioch said.
“People like Craig Millen, who opened [the Pacific Rim Hockey Academy] in Pitt Meadows right when I was getting serious about the sport — he’s an amazing teacher, and I still keep in touch with him to this day.
“I’ve made lifelong friends, who I’ll now be able to see more regularly, so that makes this deal even more exciting.”
Those relationships and that sense of community is what Wiercioch said makes hockey an ideal choice for kids who need a physical outlet.
“Anyone who chooses hockey for their physical education gets rewarded with these wonderful people and great organizations [in Maple Ridge and Pitt Meadows],” he said. “I couldn’t recommend it enough.”
Besides being proof of local hockey’s ability to create all-star athletes, Wiercioch is also part of a recent NHL trend away from big, bruising players towards a leaner, more agile body type.
At six-foot-five and 200 pounds, Wiercioch wouldn’t say his lanky-but-fit build is necessarily better than the alternative, but admitted he’s come along at the right time.
“Teams are always trying to mimic success,” he said, “so when you have a team like Pittsburgh, which is all smaller players or leaner players, and they’ve come out on top twice in a row, others follow that trend.
“You look at a few years ago when Boston won, suddenly everyone’s trying to get bulked up like they were. Now it’s the opposite. I’m sure it’ll swing the other way eventually, but for now it’s good for me.”
Wiercioch will also be bringing his scoring abilities to the Canucks — he was a regular top scorer amongst defencemen during most of his time in Ottawa — who he said were “looking to up their offence from the back end and find guys who can get pucks through.”
He’s up to not only that challenge, but also the responsibility of being a Canuck.
“I know how important the community is to these guys, and it’s something I take very seriously and passionately,” Wiercioch said.
”My wife and I have always been involved in the community no matter where we’ve gone, and this isn’t going to be any different. It’s already gone so well, we’ve really felt welcomed ever since the news came out, so we want to give some of that love back.
“Vancouver has always been my home, and it feels so great to be coming back.”


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This is the first game in the Top Gun series. For other games in the series see the Top Gun category.
|Publisher(s)||Ocean Software, Thunder Mountain, Mindscape|
|System(s)||Amstrad CPC, Atari ST, Commodore 64/128, DOS, Sinclair ZX Spectrum|
|Modes||Single player, Multiplayer|
Top Gun is a flight-combat simulation game developed by Ocean for release on several popular European home computers in 1986. The license to publish the game in the United States was largely handled by Thunder Mountain which distributed the game in 1987. The Tom Cruise film featured an F-14 Tomcat plane, and this licensed game recreates this. Viewed from the cockpit in full 3D, the game is a straight head-to-head duel, against another player or the computer. You are armed with missiles (which puts you in control of a small target zone that must remain on your opponent for 3 seconds), flares and cannons (which overheat when overused)- use these more wisely than your opponent. Pay attention to speed and altitude too. The computer has 3 planes to throw at you, of which the last is the toughest, while each player in a 2-player game also has 3 "lives".
Table of Contents

domain: hobby


Ford is recalling several models of Ford and Lincoln vehicles that were built between 2011 and 2016 due to a risk of defective door latches.
At least one accident and 3 injuries have been linked to the defect.
A door that is not securely latched can open when the vehicle is in motion, which increases the risk of injuries or a driver losing control.
The vehicles were previously recalled in 2015 and 2016 for the same issue, but Ford said the repair might not have been completed properly.
“A component within the door latches may break making the doors difficult to latch and/or leading the driver or a passenger to believe a door is securely closed when, in fact, it is not,” the recall states.
The recall involves the Ford C-Max (2013-2015), Escape (2013-2015), Ford Fiesta (2011-2014), Ford Focus (2012-2015), Ford Fusion (2013-2014), Ford Mustang (2015), Ford Transit Connect (2014-2016), Lincoln MKC (2015) and Lincoln MKZ (2013-2014).
Ford will notify owners of the recall. Dealers will inspect the latch date codes to confirm if the repair was done correctly, and replace the side door latches if necessary, free of charge.
Owners will also have the option to inspect their own vehicle’s door latches and enter the date codes into a website to determine if the latches are correct. This recall is expected to begin July 20, 2020.
Owners may contact Ford customer service at 1-866-436-7332. Ford’s number for this recall is 20S30.


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Dog lover and outdoorsman, John Marshall is a veteran reporter who has worked for The Associated Press and other major news outlets.
My Dog Has Convinced Me To Leave Up the Christmas Tree
I’ve made an important decision in life -- I’m leaving up my Christmas tree.
Though I do so with great trepidation, I feel compelled to make my choice known by reporting it in a public forum, and posting it on social media. I know by doing so I’ll be subjected to an onslaught of criticism, likely along with insulting rebukes and hateful commentary.
Many of you upon reading my revelation, will no doubt be shocked. I suspect even lifelong friends will likely call me foul and disgusting names. Other friends and former colleagues might even (gasp) unfriend me or block me from their social media accounts, or even take me off their Christmas card list.
Yes, I Have Many Concerns Over My Christmas Tree Revelation
And I have additional concerns over my revelation. I’m quite worried that when the worst of the Covid pandemic is over and I resume taking my elderly mother to church, the priest, as the Alice Cooper hit song from the 1970s goes, might “Recognize me and punch me in the nose.”
For that matter, my mother might be so alienated by my decision she’ll refuse to be seen with me. She might even write me out of her will.
If this were centuries ago I might be burned at the stake or chased down my suburban cul-de-sac by an enraged crowd wielding torches and pitchforks. If it were 150 years ago I might be rounded up by the local sheriff and his posse of vigilantes and hanged high from an oak tree. Or in more modern times, thrown in jail with no charges.
On the other hand, in revealing my decision, perhaps some of you will resolve to join me, sparking a critical and important movement that will bring much needed and momentous change to the world. At a minimum, by joining me in my painstaking decision, and mounting the courage to make your own similar stand, perhaps you’ll bring a bit of daily happiness to your life all year round.
But Don't Just Hate Me, Please Let Me Explain
Before I expand on my shocking revelation, I feel the need to at least offer up a bit of an explanation. Perhaps in doing so I’ll soften the expected fierce and ongoing attack on my character. I also hope my explanation will provide a resolve to those who desire to join me in my move.
In reaching my decision, I must say during the last few weeks my Christmas tree has prompted pleasant memories from my childhood, brought back fond memories of my father -- who was one tough SOB -- and besides, my dog likes it.
Yes, again -- I’m leaving up the tree.
Now that you picked yourself up from the floor, stopped screaming to the heavens, or concluded the pounding of your otherwise innocent desk, allow me to offer some explanations.
My Christmas Tree Prompts Pleasant Memories
First of all, it’s an artificial Christmas tree, so of course there’s no potential fire hazard.
Additionally, as I wrote above, the tree, with its fake snow on its artificial branches, evokes pleasant childhood memories. Growing up in suburban Boston, while playing hide “N seek, or some other game, likely now banned in certain school districts, I distinctly remember tromping through the snow and crawling under a large and snow covered pine tree. Being beneath the tree, with the ground covered by dried out pine needles, was actually a warm, dry and comfortable place. The pine needles made for a soft and dry ground covering, while the snow covered branches kept the area beneath the tree dry, blocked the wind and generally seemed to make for a cozy and peaceful spot.
The tree also reminds me of my father, who passed away more than two decades ago.
Here’s why. My father was a big, burly, outdoorsman, who loved being out in the woods. He was also a hunter, who since he only occasionally came home with a deer, I believe simply liked tromping around the wilderness.
It was during one hunting trip in the deep backwoods of Maine, he failed to return to my aunt’s cabin on a lake, from where he had departed earlier in the day.
With an early snowstorm blowing in, and my father not prepared for a freezing night in the woods, this caused great panic in the family.
But, as it turned out, there was no cause for panic. As my father later told me, he was tracking a deer over a distant ridgeline, when a snow squall blew in. If you’ve ever been in the deep woods late in the day, late in the year, it gets dark earlier than expected. And with falling snow, nightfall comes even sooner, visibility is reduced to near zero and the tracks of where you came from are covered up.
So, as my father recounted, he simply crawled under a pine tree and prepared to stay the night.
I knew from my childhood experiences that tree would have provided him with a soft place to sleep, kept him dry and protected him from the wind and falling snow.
The next morning, as my father told the story, he simply roused and walked down a trail. He was surprised when he was eventually greeted by a search party.
“Are you Jack Marshall?” one of the searchers gingerly asked. “Yea, who the hell are you?” my father said he responded. He couldn’t imagine what the fuss was all about.
And Besides, My Dog Likes the Tree
But here’s the final reason I’m leaving the tree up -- my dog Mookie likes it. Since I put up the tree, Mookie enjoys sleeping under it. Seeing him curled up comfortably under the tree after I first put it up, I moved his bed under it. He enjoys his naps under the tree more than any other place in the condo -- even more so than sleeping on clean towels fresh out of the dryer. (Though for the record, come bedtime Mookie still takes up his rightful position of hogging most of the bed). Perhaps he’s enjoying the cozy comfort of being under the tree, as I did as a child. Or maybe he feels safe in the protection he perceives it provides, as did that pine tree for my father during that snowy and cold night years ago in the woods of Maine.
As for me, I like the look of snow-covered branches, with a palm tree behind it. It reminds me of a wintry Southern California scene in reverse, where you can actually stand on a beach, see palm trees in the foreground, and admire snow covered mountains in the distance.
There are, however, a couple of traditions I’m conceding to. I will take off the ornaments and will remove the colored lights from the palm tree.
The Christmas tree has attached white lights, so I can’t remove those. But I am resolving not to turn them on at night during the year -- after all, I suspect the neighbors think I’m crazy as it is.
If you enjoyed reading this story, perhaps you'll enjoy this story as well.
- A Dog’s Note to Humans to Help Them Get Through the Coronavirus Crisis
Mookie, an adorable Cairn Terrier mix, had a rough life when he was younger. Rescued from hoarders in a home with no air conditioning during the roasting Texas summer, he tells his story in the hopes it will help humans get through tough times. Writt

domain: literature


Relationship seminar a success
Love was in the air in Tati Siding on Saturday as EBAT Guest Lodge hosted a successful half-day Relationship Seminar for married couples and those hoping to tie the knot.
The seminar focused on re-defining marriage as a ‘special type of a relationship’, with emphasis on the role of government in supporting and enhancing the marriage ‘institution’.
The Deputy Permanent Secretary (DPS) in the Office of the President, Ernest Phiri, delivered the keynote speech on behalf of the Permanent Secretary to the President, Carter Morupisi.
He highlighted the country’s rising rate of divorce, noting that it was becoming an increasing concern.
However, Phiri was quick to commend the government’s decision to facilitate for spouses to work in the same area to avoid tearing families apart.
“I challenge the participants and organisers to explore answers to the questions raised and find solutions to the challenges faced in relationships, especially marriages,” Phiri said, before poignantly asking, “Are we doing enough?”
The seminar was attended by a diverse group of young and veteran couples, who shared their concerns and expectations in a cordial and interactive manner, with many making moving personal testimonies aimed at growing each other.
Key issues discussed included defining a relationship and what it takes to relate with each other. Marriage as a union was also unpacked and participants were given tips on how to keep their marriages ticking. Causes of divorce and how it can be avoided were also discussed at length.
The Lead Organiser, Ogomoditse Maruapula and EBAT Consultants were encouraged to cast the net wider and reach out to the countless couples who could use such seminars to save their ailing relationships.
Speaking to Voice Entertainment, Maruapula described marriage as ‘sacred’ and urged couples to stand by the vows they made at the altar.
“Hopefully, those contemplating divorce will think more when reflecting on this relationship seminar and having heard from people that have been married for over 40 years,” Maruapula said.
A booklet on the subject was made available to all the participants.


The premiere of Fox Broadcasting Co.'s David E. Kelley drama "Ally McBeal" outperformed the season debut of its "Melrose Place" lead-in Monday and marked the best opener for any new or returning…
Demi Moore, Laurence Fishburne, Dennis Franz, Christine Lahti and Alfre Woodard have been added as presenters for Sunday's 49th annual Primetime Emmy Awards, being held at 5 p.m. at the Pasadena…
Italy's sole pay TV operation, Telepiu, controlled by France's Canal Plus, said it has reached a five-year deal with Polygram Filmed Entertainment under which Telepiu has bought exclusive pay TV…
Syndication sales veteran Sid Cohen has reupped as president of MGM Domestic TV Distribution, just as Leo gets ready to hit the syndie marketplace with a new talkshow strip for fall 1998.
National ratings for coverage of Princess Diana's funeral confirm that unprecedented numbers of U.S. viewers tuned in to the early morning memorial.
As expected, ABC prexy Preston Padden and Sinclair Broadcasting Corp. prexy David Smith have been invited to testify at the Senate Commerce Committee's Sept. 17 hearing on broadcasters' plans for the…
French pay television company Canal Plus is about to become one of the country's main cable operators by taking a majority stake in Compagnie Generale de Videocommunication (CGV).
The new-model "People's Court" and "Martha Stewart Living" got off to a good start Monday as the first wave of the season's syndie daytime crop premiered.
CBS' Eyemark Entertainment, the distributor of "Touched by an Angel" reruns, is putting the touch on cable networks, asking them to pony up $90 million for the first four years of "Angel" episodes.
E! Entertainment Television will, as previously promised, add a West Coast feed of its programming beginning Sept. 29 at midnight PDT that will enable the network to run its daily core lineup of "E…


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September 25, 2019, 4:00 PM - 6:00 PM
September 25, 2019, 11:00 AM - 4:00 PM @ White Rock Library
Meghan is introspective and open to beauty as well as the complexity and systems of nature. Her masks are about life journeys, emotions and dreams. She is inspired by humanity and the many stories that make us human.
Wednesday, September 25
Thursday, September 26
11 am – 4 pm
September 25, 2019, 1:00 PM - 4:00 PM @ White Rock Library
Join White Rock Library and Outside the Box fibre arts festival in celebrating all things mixed media. Drop by the library to enjoy the month-long art show. Partnership with Outside the Box.

domain: artistic


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*Editors note: Clarifications have been made to some parts of the original story.
Was Breonna Taylor's death connected to Louisville gentrification efforts in the Russell neighborhood? Members of the Louisville Metro Council discussed the topic at Tuesday's meeting.
A lawsuit over the summer accused a place-based investigation unit within the Louisville Metro Police Department (LMPD) of targeting 2424 Elliott Avenue, the home of Breonna Taylor's former boyfriend Jamarcus Glover. That home was also raided the night of the fatal raid on Taylor's apartment ten miles away.
In an amended lawsuit filed in Jefferson Circuit Court, Tamika Palmer and attorneys representing Taylor's estate said Louisville police targeted Glover to get the wheels moving on a "high-dollar real estate development plan for Elliott Avenue."
The 30-page lawsuit claims LMPD created a special squad, otherwise known as the place-based investigation unit (PBI), to focus on "certain areas which needed to be cleared for real estate development projects to proceed."
The suit says some of the homes on Elliott Avenue had already been demolished in previous months and that a home in the 2400 block of Elliott Avenue was in the way of efforts.
Attorneys said the home, occupied by Glover, Adrian Walker and another person, was a primary target of the LMPD’s Criminal Interdiction Division, which the PBI is allegedly under.
The lawsuit claims CID officers were "deliberately misled" to believe they were targeting some of the city’s "largest violent crime and drug rings" by going after the home's occupants, saying it would have been helpful in reducing crime and violence.
During a meeting with Louisville Metro Council members Tuesday night, Robert Kirchdorfer, the director of codes and regulations for the city of Louisville, and Jeff O'Brien, the director of Develop Louisville, said Elliott Avenue as a street was chosen for focus because of its high rates of crime and high number of vacant and abandoned properties.
"Is this the most crime-ridden street in the city?" councilman Anthony Piagentini asked during the meeting.
"I don't know if it's the most, councilman, it is one of the most," O'Brien said.
Councilman Piagentini said they needed to better understand why that home was targeted. When he asked what other spots the PBI was focusing on, both Kirchdorfer and O'Brien said they did not know.
"So once we know what other areas the PBI is focusing on, then we can look at this, then we can ask their criteria," Piagentini said.
Kirchdorfer and O'Brian said they could only speak to projects they were currently working on. The other examples they provided were defined development projects.
However, in the case of Elliot Avenue, they say it was not about development.
Piagentini said the information he gathered during Tuesday's meeting provided new leads on who may be able to provide further insight to these allegations.
"Now we have new names, we have more people we are going to invite in two weeks. They need to answer questions. They may give us names and other information that we need to follow up after that," Piagentini said.
He said members of the PBI and other city employees were included in that list of names.

domain: news


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Gravity Falls. One of the greatest American-made animated series in recent memory, it ended its second and final season with a brilliant finale in 2015. The often surreal adventures of Dipper and Mabel Pines, pre-teens on a summer vacation in the bizarre town of Gravity Falls, gained a cult following due to compelling mysteries and humor that goes over kid's heads.
Also they did a stealth crossover with Rick and Morty. (Seriously! A kids show that aired on Disney XD got away with that!)
Well, even though the series wrapped up its plotlines and purposefully ended its run, after a ludicrous amount of hiatuses and delays because no networks currently airing children's animation know how to handle serialized adventure series, it seems like creator Alex Hirsch may have more in mind with Gravity Falls thanks to these recent tweets:
Now, it's possible that Alex Hirsch is looking for fan artists who know how to do comics for something else entirely, but this does seem to strongly suggest that some sort of Gravity Falls comic project is if not officially in the works, at least being considered. Which would be fantastic. The series had a conclusive ending, but there's still room for stories set during the show's run, as well as the potential for possible post-series continuations. Which as we all know, every good TV series now gets a comic book that continues the storyline afterwards. (And some not-so-good TV series get them too!)
Now, Joe Books has recently done some Gravity Falls cine-story volumes, using screen shots of the animated series to make a pseudo-comic, so it's likely that they have the comic continuation rights to Gravity Falls. Given their commitment to fan favorites like having Amy Mebberson on Disney Princesses or the current ongoing Darkwing Duck series, I'm pretty sure Gravity Falls would be in good hands there.
If it is going there, which we don't know. But I'll keep my fingers crossed.
Peter S. Svensson has a pretty sweet Soos costume he's brought to a few conventions. He'll be attending more conventions for Bleeding Cool this year, so keep an eye out for him!


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Place: Embassy Suites Riverwalk
Q&As with the experts, Part 5
- Staff San Antonio Business Journal
Randy Griffin , CEO of Armada Oil Inc.
Q: How has unconventional shale drilling changed your company in the last decade?
A: Unconventional drilling has opened up a whole new world of opportunities for junior oil-and-gas companies. Many have been fortunate enough to acquire acreage early in new unconventional plays and those acreage position have become company makers. Others have been able to joint venture or otherwise participate in the drilling of those plays. Armada has focused on the Niobrara Shale in Wyoming and has acquired a 9,800 farmout from Anadarko Petroleum. We expect to begin drilling on that acreage position later this year.
Q: Where do you stand on LNG exports and crude oil exports?
A: I believe that we should pursue all avenues to export LNG and crude oil, especially LNG. We have a tremendous supply of natural gas and should take advantage of foreign markets to the best of our ability.
Q: What’s the hottest play for Armada Oil, Inc. right now?
A: We have a newly acquired producing property in Kansas on which we expect to drill additional wells this year. Beyond that, the Niobrara Shale in northern Colorado and southern Wyoming is our primary focus.
Q: What is the single biggest issue facing your company right now?
Q: Finding new sources for drilling capital is an ongoing challenge for junior oil-and-gas companies.
Niloufar Molavi , U.S. energy leader and Greater Houston market managing partner for PricewaterhouseCoopers LLP
Q: How have the views on liquefied natural gas shifted?
A: We were building LNG terminals to import, and now we’re reconfiguring them to export. We’re even talking about exporting crude. The trend is going from the West to the East. The U.S. could (eventually) serve the needs of places like China, where you have the rise of the middle class.”
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domain: geography


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West Ham slip to narrow defeat at champions Leicester City
PUBLISHED: 17:00 31 December 2016 | UPDATED: 18:22 31 December 2016
PA Wire/PA Images
The Hammers had their chances but their excellent run of winning form came to an end at the King Power Stadium
Leicester City 1 West Ham United 0
West Ham’s excellent run of three consecutive wins came to an end at champions Leicester City, but they can count themselves slightly unlucky as they went so close to grabbing at least a point from this game.
Sloppy defending allowed Islam Slimani to score what proved the winner in the first half, but the Hammers dominated after the break and should have taken advantage of the chances they carved out to earn something from the match.
Hammers boss Slaven Bilic stuck with the same team that had battered Swansea City 4-1 on Boxing Day, with Pedro Obiang, back from suspension and Manuel Lanzini back from injury both taking their place on the bench.
But it was the chanpions who started the brighter. Having lost at home to Everton on Boxing Day, they were keen to put that right and carved out two early chances.
First Riyad Mahrez got on the end of a cross to shoot, only for Darren Randolph to push the ball over the top and then a couple of minutes later, Marc Albrighton crossed to the far post for Islam Slimani whose header came off the outside of the post.
West Ham rode that storm and went close themselves when Aaron Cresswell swung in a superb cross which beat three defenders and fell for Michail Antonio at the far post, only for his effort to fly over the bar.
But the pressure was soon back on to the West Ham defence with Havard Nordtveit in particular having a tough time at right back.
On 20 minutes the champions broke the deadlock. Albrighton got down the right again and this time his cross picked out a more central Slimani who headed firmly past Randolph to make it 1-0.
Leicester could have added to that tally with shots by Slimani and Demarai Gray blocked, while at the other end, Antonio did get beyond the full-back to cross for Andy Carroll, but the nig man did not the best of connections.
West Ham went even closer on 37 minutes. Payet’s shot at the far post was saved and an almighty scramble ensued which saw Ayew and Mark Noble both fail to put the ball over the line.
With five minutes to go until half time, West Ham were certainly making inroads into the Leicester defence and after Antonio had shot straight at Kasper Schmeichel, he then went even closer as he efforts smashed against the woodwork.
In first-half injury time both teams came close to scoring. First Payet was fouled by Robert Huth just outside the box and his free kick was superbly saved by Schemeichel and from the ensuing break, Angelo Ogbonna was booked for a foul on Gray and from the resulting free kick, Mahrez brought out an excellent save from Randolph.
West Ham looked to push on after the break and with Leicester looking a little nervous, it was the visitors who began to press without really testing the keeper.
Bilic threw on Manuel Lanzini for the hobbling Noble and he almost immediately put in Carroll with a long pass which was hastily cleared by Huth.
Aaron Cresswell was denied by a smart save after his low free kick was deflected towards the far corner, while Cheikhou Kouyate also went close as West Ham began to put the pressure on.
It was certainly an end-to-end finish as Payet forced an easy save, while at the other end, Gray shot over the bar as Leicester broke away.
Cresswell then delivered a superb cross to the head of Carroll, but the bug man put his effort inches wide as time ran out for the Hammers.
It was Leicester who almost had the final word as Ben Chilwell broke into the box ajnd let fly only for first Carroll and then Randolph to foil his effort.
In the end, West Ham simply ran out of steam and they must now turn their attention to Monday’s home clash with Manchester United.
West Ham United: Randolph, Nordtveit. Reid, Ogbonna, Cresswell; Kouyate (Fernandes 78), Noble (Lanzini 57); Antonio, Payet, Ayew (Feghouli 65) ; Carroll.
Unused subs: Adrian, Obiang, Fletcher.
Booked: Ogbonna, Nordtveit, Lanzini.
Referee: Anthony Taylor.
If you value what this story gives you, please consider supporting the Newham Recorder. Click the link in the orange box above for details.


text:
LynxDx, a Michigan diagnostics laboratory specializing in prostate most cancers screening and danger prediction, acquired liquid dealing with options from INTEGRA Biosciences to streamline its pattern preparation for top throughput COVID-19 testing.
The lab is now within the means of transferring these merchandise over to prostate most cancers screening, the place they’re serving to to enhance consequence accuracy and turnaround occasions and, finally, help well timed prognosis and remedy. LynxDx performs prostate most cancers screening utilizing its proprietary MyProstateScore 2.0 (MPS2) strategy, a non-invasive urine check that detects the presence of 18 genetic biomarkers linked to prostate most cancers. MPS2 makes use of a cohort-specific algorithm to optimize diagnostic accuracy and supply scientific flexibility for biopsy naïve sufferers and sufferers with a previous unfavourable biopsy, offering extremely correct and particular insights that may scale back the frequency of pointless and doubtlessly high-risk biopsies.
In early 2020, LynxDx adopted 4 VIAFLO 96 handheld electronic pipettes and 4 VOYAGER adjustable tip spacing pipettes from INTEGRA to permit it to shortly transition over to high-volume COVID-19 testing. Dr. Jacob Meyers, Senior Scientist of Analysis and Improvement at LynxDx, defined: “We selected INTEGRA’s VIAFLO 96 and VOYAGER pipettes as a result of we would have liked sturdy and dependable programs that might permit us to persistently deal with massive numbers of extremely variable saliva samples for correct and speedy outcomes. These versatile merchandise performed a key function in enabling us to keep up quick turnaround occasions for COVID-19 testing, in addition to to handle an elevated workload as our most cancers screening operations have steadily obtained again on observe over the latest months.”
We’re already utilizing the VOYAGER pipettes in our most cancers screening workflow, and we’re presently performing validation of the VIAFLO 96 pipettes for this utility. We’d additionally like to amass extra INTEGRA merchandise within the close to future, as these pipetting options will permit us to additional streamline and automate our liquid dealing with workflows in preparation for the diagnostic wants of the longer term,” Jacob concluded.
Go to the INTEGRA Biosciences web site to study extra.


text:
Litecoin price has been in a tight range in the past few days as the market participants waited for the next catalyst. LTC has risen in the past two straight days and is trading at $54.76, where it has been in the past few days. It remains about 19.3% below the highest level in September.
Litecoin consolidation continues
Litecoin had a mild performance in September even as market conditions remained volatile. In September, American stocks crashed by more than 10%, with indices like the Dow Jones, Nasdaq 100, and S&P 500 retreating.
At the same time, the US dollar index (DXY) surged to the highest level in more than 20 years. Other key indices signaled at potential risks in the market. For example, the closely watched VIX index, which is a measure of volatility, soared to more than $32 while the fear and greed index dropped to the fear zone.
All these numbers signal that Litecoin did well in a difficult market. The same is true for other cryptocurrencies like Bitcoin and Ethereum.
The main catalyst for Litecoin in September was Ethereum Merge, which transitioned it from a proof-of-work (PoW) to a proof-of-stake (PoS). Since this was the biggest event in the blockchain industry, most cryptocurrencies reacted to it.
Looking forward, there will be no major cryptocurrency-focused event of the magnitude of the Merge in October. Therefore, investors will continue to focus on the broader market issues like inflation and monetary policy.
This week, the US will publish the latest jobs numbers. Analysts expect the data to show that the country’s economy added more than 300k jobs in September. As such, with inflation at an elevated level, there is a likelihood that the Fed will continue hiking interest rates.
Litecoin price prediction
The daily chart shows that the LTC price has been in a consolidation phase in the past few days. It has remained between the important support and resistance levels at $46.3 and $67.35. The coin is still hovering at the 25-day and 50-day moving averages while the Relative Strength Index (RSI) has moved to the neutral point at 50.
Therefore, there is a likelihood that Litecoin will remain in this tight range in October. A move above the resistance at $67 will mean that there are more buyers left while a drop below the support at $46 will mean that there are more sellers in the market.
How to buy Litecoin
Binance is one of the largest cryptocurrency exchanges in the world. It is better suited to more experienced investors and it offers a large number of cryptocurrencies to choose from, at over 600.
Binance is also known for having low trading fees and a multiple of trading options that its users can benefit from, such as; peer-to-peer trading, margin trading and spot trading.
FP Markets combines metals, indices connectivity and award-winning execution speed with institutional-grade liquidity to provide traders consistently tighter spreads and unparalleled trading conditions. FP Markets offers Forex, Equities, Commodities, Cryptocurrency, Futures and Index CFDs all from one account.

domain: economics


text:
Nephrology and dialysis centre
In the dialysis centre, with its 23 places and two additional places in the intensive care unit, we carry out up to 8,500 haemodialyses a year, making us one of the largest centres in the canton of Zurich.
We offer you excellent medical care, based on comprehensive expertise and many years of experience. At the same time, we attach great importance to holistic and ongoing care, clear communication and individual attention. Our aim is to give you a strong sense of security and trust. Our experienced nursing staff will look after and accompany you with great empathy during your regular visits to the dialysis centre. Each patient is also assigned a personal carer as part of our reference care system.
Our range of outpatient and inpatient treatments
- Haemodialysis, haemodiafiltration (ONLINE-HDF 5008 Fresenius)
- Acute haemodialysis
- Peritoneal dialysis (peritoneal dialysis)
- Holiday and guest dialysis, also for hepatitis B and C or HIV patients
- Catch-up dialysis for people from outside the hospital who are hospitalised in our hospital or in a rehabilitation clinic
We also offer special services for our dialysis patients, such as aroma care and nutritional counselling.
Relaxed holidays despite dialysis - that's what you get with us! As a rule, we have sufficient capacity. Nevertheless, we recommend that you book early. For patients from abroad, especially from EFTA countries, dialysis is billed directly to the compensation fund. Our team offers comprehensive care for all patients, including those with hepatitis B, hepatitis C and HIV. If you have any further questions, please do not hesitate to contact us by e-mail or telephone.
Head of the care team of the dialysis centre
Our nursing team of 22 dedicated employees is led by Silvana Tenini, Ward Manager of the Dialysis Centre, and Anke Haas, Deputy Manager of the Dialysis Centre. The majority of our specialists have been part of our team for many years and have extensive, long-term experience.
Monday to Thursday:
8.00 a.m. - 12.00 p.m.
13.00 - 16.00
8.00 - 12.00
Nephrologie und Dialysezentrum


text: Introducing one of RC Pet Products' newest and most exciting products, Bark Notes. Bark Notes is a simple communication tool that can help pet guardians share their pet's key personality traits from a distance. Is your dog working? Is she friendly? Scared of other dogs? Help other guardians "get the message".

domain: pet


text:
In an effort to allow the greatest availability of appointments to our patients, Specialty Natural Medicine is updating its late cancellation and no show policies.
Effective June 1st, 2016 all late cancellations will be charged a $75 fee and no show’s will result in a $100 fee, no exceptions*.
A late cancellation is any cancellation or reschedule attempt made less than 24 hours before the scheduled appointment time. A “no show” is any appointment where the patient does not arrive within 10 minutes of the scheduled appointment start time. This policy applies to appointments even if they are made within 24 hours of the actual appointment and subsequently cancelled or missed.
Allowing waivers for no shows or late cancellations has recently resulted in a high volume of late cancellations, impacting the ability of patients to be seen by the Doctor of their choice at their time of choice.
We have systems in place to send courtesy reminders based on the patient’s preference. Please note that these reminders are a courtesy and the patient is solely responsible for arriving on time for their scheduled appointments.
* - Apple Health patients are exempt from no show and late cancellation fees. Please note that Apple Health patients who no show or late cancel are subject to immediate dismissal from the practice.


text:
From Seed to Plant
by Gail Gibbons Year Published:
Informational text for grades 2-3 From Seed to Plant follows the life cycle of a plant. Beginning with an explanation of different types of seeds, the book continues with a simple explanation of pollination. Germination is also explained and illustrated. The book ends with mature plant that is ready to continue the cycle with new seeds.

domain: agronomy


text:
U.S. release date
Shang-Chi and the Legend of the Ten Rings
September 3, 2021
Kevin Feige and Jonathan Schwartz
November 5, 2021
Kevin Feige and Nate Moore
Spider-Man: No Way Home
December 17, 2021
Kevin Feige and Amy Pascal
14 hours ago ˇ Marvel Studios has a full slate of upcoming MCU movies, and many have ... Related: Marvel's New Phase 4 Slate: Every MCU Movie Release Date (2021- 2023).
These are the 27 new Marvel movies and shows coming in 2021 ...
www.joe.ie › movies-tv › 26-new-marvel-movies-shows-coming-2021-bey...
20 hours ago ˇ These are the 27 new Marvel movies and shows coming in 2021 and beyond. ... Fans of Marvel have A LOT to get excited about... 2020 was the first time in over ...
People also ask
Will there be a marvel Phase 5?
As the MCU starts a new chapter in Phase 4, Marvel Studios is already teasing a brand-new Avengers team through The Falcon and the Winter Soldier. MCU Phase 4 is just beginning, but Marvel Studios is already teasing a new Avengers team for Phase 5 as early as now.
Is Avengers 5 confirmed?
The fact that Avengers 5 hasn't been solidly confirmed yet and with filming still to be done on upcoming Marvel movies, all of which feature characters likely to appear in Avengers 5, makes us think a 2023 release could be too early.
Is Marvel Phase 4 confirmed?
Feige indicated Secret Invasion and Ironheart would tie-in with future MCU films. These new Disney+ series, plus Black Panther II, Captain Marvel 2, Ant-Man and the Wasp: Quantumania, Guardians of the Galaxy Vol. 3, and Fantastic Four were confirmed to be part of Phase Four.
What Marvel movies are coming out in 2023?
More recently, the studio announced the first two MCU blockbusters that fans will see in 2023. Ant-Man and the Wasp: Quantumania and Guardians of the Galaxy Vol. 3 are kicking off that year, but Disney already had two additional spots on its 2023 release schedule for mystery Marvel movies.
13 hours ago ˇ Although the Marvel Cinematic Universe continues to dominate the global box office, there are many upcoming Marvel movies—from Sony Pictures and Disney's ...
9 hours ago ˇ The Marvel Cinematic Universe (MCU) is an American media franchise and shared universe ... The films of Phase Four will begin with Black Widow (2021). ... several superhero films with Columbia Pictures, New Line Cinema and others, ...
25 Results ˇ 10 hours ago ˇ Browse new Marvel comics in the official Marvel comics release calendar. View this week's new comic issues, and find out where to read them!
the new avengers movie 2021 - Bikers Against Drunk Drivers
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21 hours ago ˇ find a new favorite in 2021. Note: This detailed upcoming Disney+ Marvel movie and show guide has a ton of Avengers Endgame spoilers sprinkled all over. Read ...
Marvel's 'Eternals' Looks 'Different' - Here's Why Fans Think That's a ...
www.cheatsheet.com › Movies
8 hours ago ˇ June 12, 2021. All signs lead to the upcoming Marvel's Eternals movie being a departure from other MCU movies. Not only has very little been said about this ...


text:
THE three levels of government added 1500 workers in Tasmania last financial year.
That is according to Australian Bureau of Statistics figures, which suggest governments kept on boosting their Tasmanian payrolls as private sector jobs vanished.
It looks like more than 3000 private sector jobs vanished in Tasmania in the year to the end of June.
The ABS found Tasmania had 49,000 public sector employees at the end of June, up from 47,500 a year before.
Other ABS figures showed the state lost 1700 jobs overall in the year to the end of June.
Run together, the two sets of figures suggest the private sector lost about 3200 jobs for the year.
If so, the pain was largely masked by big growth in the government sector.
According to the ABS:
Local government added 700 employees for the year, for a total of 4400;
the state sector added 700, despite public service cutbacks, to 39,500; and,
Commonwealth employment in Tasmania added about 100, to 5100.
About 21.4 per cent of Tasmanian employees were public sector employees at the end of June. Nationally, the figure was about 16.25 per cent.
The total public sector annual payroll in Tasmania was up by $24.6 million to $3.404 billion.
The state sector payroll fell by about $32 million, suggesting most of the jobs growth came in part- time and/or lower paid work.
Local government payrolls shot up by $35.2 million for the year to $216.7 million.
The figures cover government- owned trading enterprises, but do not include a few areas on the public payroll, including defence.

domain: politics


text:
|تعداد مشاهده مقاله||111,603,623|
|تعداد دریافت فایل اصل مقاله||86,230,339|
Molecular detection of Toxoplasma gondii infection in aborted fetuses in sheep in Khorasan Razavi province, Iran
|Iranian Journal of Veterinary Medicine|
|مقاله 5، دوره 11، شماره 2، تیر 2017، صفحه 147-154 اصل مقاله (896.83 K)|
|نوع مقاله: Clinical Pathology|
|شناسه دیجیتال (DOI): 10.22059/ijvm.2017.61709|
|Laleh Danehchin1؛ Gholamraza Razmi* 2؛ Abolghasem Naghibi1|
|1Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran|
|2Center of Excellence in Ruminant Abortion and Neonatal Mortality, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran|
|Background: Toxoplasma gondii is a significant cause of abortion in sheep and goats in the world. Toxoplasmosis is caused reproduction disorders such as fetal resorption, early embryonic death, mummification, abortion, stillbirth, neonatal and fetal death in sheep . Objectives: The aim of this study was to detect T. gondii infection in ovine aborted fetuses in Khorasan Razavi province. Methods: From June 2009 to July 2013, 112 brain samples of aborted ovine fetuses were collected and examined to detect T.gondii DNA by nested- PCR. The association the frequency of T.gondii infection with age and geographical location of aborted fetuses were also studied. Results: The results showed that 18 (16.07%) of brain samples of aborted fetuses were Toxoplasm positive in PCR reaction . The frequency of T.gondii in the age group ≥120 day was more than other age groups of infected aborted fetuses (P<0.05) . All the infected fetuses belonged the sheep flocks in the northern part of province. Conclusion: The results showed the moderate T.gondii infection among ovine aborted fetuses in the northern part of Khorasan Razavi province.|
|abortion؛ Brain؛ Nested-PCR؛ sheep؛ Toxoplasma gondii|
|عنوان مقاله [English]|
|تعیین آلودگی ملکولی توکسو پلاسما گوندیی در جنین های سقطی گوسفندان استان خراسان رضوی|
|لیلا دانه چین1؛ غلامرضا رزمی2؛ ابوالقاسم نقیبی1|
|1دانش آموخته دکتری انگل شناسی ، دانشکده دامپزشکی دانشگاه فردوسی مشهد|
|2قطب سقط چنین و بیماری نوزادان نشخوارکننده ، دانشکده دامپزشکی دانشگاه فردوسی مشهد|
|زمینه مطالعه : توکسوپلاسما گوندی یک عامل مهم سقط جنین در گوسفندان و بزان در دنیا می باشد . توکسوپلاسموز سبب اختلالات تولید مثل در جنین از جمله جذب جنین ، مرگ زودرس جنین ، مومیایی شدن ، سفط چنین ، مرده زایی و مرگ نوزادان در گوسفندان می شود. هدف : هدف این مطالعه شناسایی آلودگی توکسوپلاسما گوندیی در جنین های سقطی گوسفندان استان خراسان رضوی می باشد. روش کار: ازخرداد 1389 تا تیر ماه 1392 تعداد 112 نمونه مغز جنین های سقط شده گوسفندان جمع آوری و مورد آزمایش nested- PCR جهت تعیین آلودگی توکسوپلاسما قرار گرفتند. ارتباط فراوانی آلودگی توکسو پلاسما گوندی با فاکتور سن و منطقه جغرافیایی مورد بررسیقرار گرفت . نتایج: نتایج آزمایش PCR نشان دهنده آلودگی 18نمونه مغزی (8/16%) به توکسوپلاسما بودند. در این مطالعه فراوانی توکسوپلاسما در جنین های سقط شده بالای 120 روز در مقایسه با سایر سنین بالاتر بود (P<0.05). تمام جنین های سقط شده آلوده به توکسو پلاسما متعلق به مناطق شمالی استان خراسان رضوی بودند. نتیجه گیری : نتایج بدست آمده نشان دهنده آلودگی نسبتا بالای توکسوپلاسما گوندی در جنین های سقطی مناطق شمالی استان خراسان رضوی می باشد.|
|سقط جنین, مغز, Nested-PCR, گوسفند, توکسوپلاسما گوندیی|
Toxoplasma gondii was recognized for the first time as a significant cause of abortion in sheep in New Zealand and later in many countries (Buxton et al. 2007; Dubey, 2009). T.gondii infection during pregnancy can result in embryo/early fetal loss, fetal death and abortion/mummification and birth of weak lambs. (Buxton 1991; Scott 2007). This parasite is transmitted to sheep via ingesting food and water contaminated with oocysts which are excreted by cats or by other transmission rout from mother to fetus (Buxton et al. 2007). Furthermore, recent molecular studies showed that repeated transmission of T. gondii from dam to fetuses may be more common than previously observed in sheep (Duncanson et al. 200l; Morley et al. 2007; Hide et al. 2009).
The diagnosis of T. gondii infection is usually based on serological assay, histopathological examination and mouse bioassay. Many epidemiological studies have been carried out on ovine toxoplasmosis in different regions of Iran. The seroprevalence of ovine toxoplasmosis was reported between 13.8% -35% in different areas of Iran (Ghorbani et al. 1983; Rahbari&Razmi1992; Hashemi-Fesharki et al. 1996; Sharif et al. 2007). T.gondii infection was also detected in ovine fetuses using PCR and bioassay methods from Iran (Rassouli et al. 2011; Razmi et al. 2010; Zia-ali et al. 2007; Habibi et al. 2012). The aim of this study was to determine the frequency of Toxoplasma infection in aborted ovine fetuses in the province using molecular method.
Materials and Methods
The field of study: Khorasan Razavi province is located in northeastern Iran between 33°30′-37°41′ N latitude and 56°19′-61°18′ E longitude, with an area of more than 127,000 km2. The northern part of the province has a mountainous climate and suitable conditions for agricultural activity and animal husbandry. The southern part is mostly semi-desert and desert, with poor vegetation cover.
Sampling: From June 2009 to July 2013, one hundred-twelve aborted ovine fetuses were examined. The fetuses and fetal membranes were grossly examined for any macroscopic lesions and the age of the aborted fetuses was also determined by crown-rump length (Evans & Sack 1973).
The fetuses were necropsied and the brain samples were collected and stored at −20 °C for PCR analysis. The frozen fetal brain tissues were homogenized by mortar and pestle in Tris-HCl (pH 8•0) followed by treatment with proteinase K (Fermentas®, Lithuania) at a final concentration of 200 μgml. The phenol-chloroform and ethanol precipitation method was used for DNA extraction procedures. The purified DNA samples were resuspended in 20 μl of TE (10mM Tris and 1mM EDTA, pH8•0) and stored at −20 °C. DNA extracted from 106 particles of the RH strain of T. gondii was used as a source of positive control sample. DNA samples were tested by nested-PCR based on the amplification of B1 gene (35 copies per parasite) using 2 sets of oligonucleotide primers as was described by Burg et al. (1989). Amplification was performed in 20 μl reaction volumes (Accupower PCR premix kit,Bioneer®, South Korea) with a final concentration of each dNTP of 250 μM in 10mM Tris-HCl pH 9•0, 30mM KCl and 2mM MgCl2, 1U Taq DNA polymerase and 10 pmol of each PCR primer(Denazist. Iran). Then 1 μl of DNA template (250-500 ng) was added to each reaction and the remaining 20 μl reaction volume was filled with sterile distilled water. The PCR conditions were slightly modified. The following cycling conditions using a Bio-Rad thermocycler were applied: The first step of amplification was 3min of denaturation at 94 °C, this step was followed by 38 cycles, with 1 cycle consisting of 1min at 94 °C, 1min of annealing at 50°Cfor each pair of primers, and1min at72 °C, an extension step of 7min at 72 °C was planned after the eventual cycle. The PCR primer pair was derived from the B1 gene sequence (Burgetal.1989) , B1F0:5′GGAACTGCATCCGTTCATGAG3′ and BIR0:5′TCTTTAAAGCGTTCGTGGTC-3′ (position 694-714 and 887-868 nucleotide respectively).The PCR products were electrophoresed through a 1.8% agarose gel to assess the presence of a 193 bp band (if any) in the first round. All PCR products were included in the second round of PCR. The nested-PCR reaction condition was similar to the first round, except for the annealing temperature which was 52 °C, the number of cycles was 30. The resulted amplification products were diluted to 1:10 in water and then a second amplification was done with the internal primers (Burg et al 1989), B1F1:5′-TGCATAGGTTGCAGTCACTG-3andB1R1:5′-GGCGACCAATCTGCGAATACACC-3′ (position 757-776 and 853-831 nucleotide , respectively), using 1µl of the diluted product as the template nested PCR. The amplified products were detected in 1.8% gel stained by electrophoresis and viewed under ultraviolet light.
The presence of specific bands of 193 bp in primary PCR and 96 bp in nested -PCR on agarose gel was considered as positive sample.
Statistical analysis: The Chi-square test was used to analyze the association between the T.gondii infection with fetal age and geographic region.
All risk factors were analyzed in a multivariable logistic regression model and Odds-ratios (OR) with 95% conﬁdence intervals are calculated. Statistical analyses were performed with the statistics package SPSS, version 16.0 for Windows (IBM, New York, USA).
T.gondii DNA was detected in 18 out of 112 (16.07%) brain samples of ovine fetuses by nested- PCR (Table.1) Three positive samples demonstrated a detectable reaction in both rounds of PCR (Fig.2) and 15 positive samples were detected in second round (Fig. 3). Based on the geographical location, all Toxoplasma infection was detected in the aborted fetuses of sheep in the north areas (p<0.01, Table 2). In this study, significant differences were found between Toxoplasma infection in different age groups of fetuses (p<0.05, Table 2). No significant difference was shown between the frequency of Toxoplasma infection in different time periods (p>0.05, Table2).
Table 1 –Frequency of T.gondii infection in aborted fetuses of sheep in different areas of Khorasan province
Table 2: . Frequency of T.gondii infection in aborted fetuses of sheep from the Khorasan Razavi Province, Iran, in relation to possible risk factors.
Several PCR methods have been developed for T. gondii detection in ovine abortion cases, (Hurtado and Aduriz 2007). Among these methods, the B1 gene is widely used as a target in many Toxoplasma PCR detection methods, mainly as a nested protocol based on that described by Burg et al (1989). In the present study, T. gondii infection was detected in 16.07% of aborted ovine fetuses by nested PCR.
Among the regions studied in Rio Grande do Norte, seroprevalence varied from 17.8% in the Central Potiguar region to 26.3% in the Leste Potiguar region (24).
In Iran, T.gondii DNA has been detected in aborted fetuses to range between 13.5% to 69% in Khorasan, Qazvin and East Azarbeijan provinces by different PCR assays (Rassouli et al. 2009; Habibi et al. 2011; Mooazeni Jula et al. 2013). These results show that the moutainous climate of Qazvin and East Azarbeijan provinces are more favorable conditions to the development and maintenance of T. gondii oocysts in the environment. Oocysts can survive in the environment for months, depending on moisture and temperature. Thus, low humidity and high temperatures of the Khorasan Razavi province is deleterious to the viability of Toxoplasma oocysts in the pasture. In other countries, T. gondii PCR positivity was detected to range between 3.5 and11.1% in Italy (Masala et al. 2003; Pereira-Bueno et al. 2004; Chessa et al. 2014)., 5.4 to16.9% in Spain (Hurtado et al. 2001, Moreno et al. 2012), 14% in Ireland (Gutierrez et a l. 2012), 14.3% in Brazil (de Moraes, et al. 2011) and 29.8% in Jordan (Abu-Dalbouh et al. 2012). The differences of PCR results would be related to many factors such as different PCR methods, climatic condition in each country.
All infected fetuses in this study belonged to the areas that are located in the northern part of the province. The northern part of the province has mountainous climate and mean annual rainfall is more than the southern areas with semi desert climate. The mountainous climate confers favorable conditions for Toxoplasma oocysts survival, because the viability of T. gondii oocysts is dependent on the moisture and temperature of the soil (Lélu et al. 2012; Du et al. 2012 ;). The higher prevalence of ovine toxoplasmosis has been reported in the humid and temperate areas compared to dry areas (Kamani et al. 2010; Alvarado-Esquivel et al. 2013; Gebremedhin et al. 2013; Andrade et al. 2013; Hamidinejat et al. 2008).
In the present study, most of the infected fetuses had more than 120 days of age. The clinical signs of ovine toxoplasmosis in pregnant ewes depends on the age and immune status of fetus. Fetal death is more likely to occur from infection during the first trimester of pregnancy when the fetal immune system is relatively immature. Infection at mid-gestation can result in birth of a stillborn or weak lamb which may have an accompanying small mummified fetus, whereas infection in later gestation may result in birth of a live, clinically normal, but infected lamb (Buxton 1991; Scott 2007; Innes et al. 2009). Other studies have shown that the abortion associated ovine toxoplasmosis generally occurs during the mid pregnancy (Pereira-Bueno et al. 2004) when the T. gondii aborted fetuses have 110-130 days of age (Giadinis et al. 2011).
Based on our results, the moderate frequency of T.gondii infection was detected among ovine aborted fetuses in this area. However, a definite diagnosis of Toxoplasma infection in causing abortion in sheep needs to combine pathological and molecular examination
Conflict of interest: The authors declare that they have no conflict of interest
This study was supported by a grant VPRTFM-3.27748 from the Vice President of Research and Technology of Ferdowsi University of Mashhad, Iran. We would like to thank Dr Zahra Naseri for help sampling and Dr Fazaeli kindly provided the control positive PCR from the Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, Iran.
Abu-Dalbouh, M.A., Ababneh, M.M., Giadinis, N.D., Lafi, S.Q. (2012) Ovine and caprine toxoplasmosis (Toxoplasma gondii) in aborted animals in Jordanian goat and sheep flocks. Trop Anim Health Prod. 44: 49-54.##
Alvarado-Esquivel, C., Estrada-Malacón, M.A., Reyes-Hernández, S.O., Pérez-Ramírez, J.A., Trujillo-López, J.I.,Villena, I., Dubey, J.P. (2013) Seroprevalence of Toxoplasma gondii in domestic sheep in Oaxaca State, Mexico. J Parasitol. 99: 151-2 .##
Andrade, M.M., Carneiro, M., Medeiros, A.D., Andrade Neto, V., Vitor, R.W. (2013) Seroprevalence and risk factors associated with ovine toxoplasmosis in Northeast Brazil. Parasite. doi: 10.1051/parasite/2013019.##
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de Moraes, E.P., da Costa, M.M., Dantas, A.F., da Silva, J.C., Mota, R.A. (2011) Toxoplasma gondii diagnosis in ovine aborted fetuses and stillborns in the State of Pernambuco, Brazil. Vet Parasitol. 183: 152-5.##
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Habibi, G.R., Imani, A.R., Gholami, A.R., Hablolvarid, A.R., Behroozikhah, A.M., Lotfi, M., Kamalzade, M., Najjar, E., Esmaeil-Nia, K., Bozorgi, S. (2012) Detection and Identification of Toxoplasma gondii Type One Infection in Sheep Aborted Fetuses in Qazvin Province of Iran. Iran J Parasitol. 7: 64-72.##
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Hurtado, A., Aduriz, G., Moreno, B., Barandika, J., García-Pérez, A.L. (2001) Single tube nested PCR for the detection of Toxoplasma gondii in fetal tissues from naturally aborted ewes. Vet Parasitol. 102: 17-27 .##
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Innes, E.A., Barley, P.M., Buxton, D., Katzer, F. (2009) Ovine toxoplasmosis. Parasitol. 136: 1887-1894.
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Lélu, M., Villena, I., Dardé, M.L., Aubert, D., Geers, R., Dupuis, E., Marnef, F., Poulle, M.L., Gotteland, C., Dumètre, A., Gilot-Fromont, E. (2012) Quantitative estimation of the viability of Toxoplasma gondii oocysts in soil. Appl Environ Microbiol. 78: 5127-32.##
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Leah and her husband Ross live on a beautiful little farm out in Iowa. Up until recently their farm was considered more of a hobby farm than a productive farm. However in the next few months Leah is excited to return to work and Ross will be turning their hobby farm into a family business. They're hoping to start off with selling their own farm fresh eggs and veggies.
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I love this couple and their sweet farm. You can find out more about Harvesting Roots at theirblog.


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Margaret Annette McCrie Johnston
10 August 1914
Sydney, New South Wales, Australia
|Died||19 June 2002 87) (aged|
Kingston upon Thames, London, England
(m. 1946;died 1974)
|Relatives||Angela Scoular (niece)|
Margaret Johnston (10 August 1914 – 19 June 2002) was an Australian actress. Johnston was best known for her stage performances, but also appeared in 12 films and a handful of TV productions before retiring from acting in 1968 to devote herself to running a theatrical agency.
Johnston was the second of three daughters born in Australia to English parents. She was educated at the North Sydney Girls High School, then at the University of Sydney where she studied Law.  Johnston had shown an interest and aptitude for drama from an early age acting in various school productions before working professionally in Sydney's theatres. It was reported that her oft-stated ambition at this time was "to move to Europe, to learn her craft, and to lose her (Australian) accent."  In 1936, Johnston (accompanied by her sisters) travelled to Britain in order to pursue her career. She was accepted as a student by the Royal Academy of Dramatic Art, where she was tutored by Stefan Hock.
Johnston's first role on the West End stage was a minor part in a 1939 production of Saloon Bar, a comedy-thriller. She followed this with a period in repertory theatre, before being cast in a 1942 production of Murder Without Crime at London's Comedy Theatre. A box-office hit, the production ran for over a year. In 1944 Johnston played opposite Fay Compton in The Last of Summer at the Phoenix Theatre. Her performance, especially in an acrimonious verbal duel with Compton at the play's climax, was much praised and drew the attention of influential theatrical impresario Binkie Beaumont, who signed her for his agency H.M. Tennent Ltd., then London's leading theatrical management company.
In 1946, Johnston played the lead role of Kitty Duval in The Time of Your Life at the Lyric Hammersmith, followed in 1948 by a stage production of The Barretts of Wimpole Street in which she portrayed Elizabeth Barrett, a performance which earned her further critical appreciation, although the production itself was thought lacklustre. Critic Alan Dent noted that "the one exception among the general cowed lethargy of the Barretts is Elizabeth herself...she is sincere, touching, awakened."
Johnston appeared as Alma Winemiller in a 1951 production of Tennessee Williams' Summer and Smoke , initially at the Lyric then transferring to the Duchess Theatre.  This performance drew some of the best notices of her career, with the Daily Herald saying Johnston "...gave a performance of tender brilliance. It was her best work to date", while the Daily Mirror described her as "probably our finest young actress".
From 1956 Johnston appeared in several productions at the Royal Shakespeare Theatre in Stratford-upon-Avon, playing Desdemona in Othello , Portia in The Merchant of Venice and Isabella in Measure for Measure . Two roles in 1959, in Sugar in the Morning at the Royal Court and a rare venture into domestic comedy with The Ring of Truth at the Savoy Theatre, marked her last stage appearances until 1966, when she starred as Lady Macbeth in Michael Benthall's production of Macbeth at the Chichester Festival Theatre in what would prove to be her last stage role.
Johnston made her screen debut in the film The Prime Minister (1941), in a supporting role as one of the daughters of Benjamin Disraeli, played by John Gielgud. Following her stage successes between 1942 and 1944, she returned to film in the Rex Harrison star-vehicle The Rake's Progress (1945), with the film critic of London's Evening News noting: "Fair-haired, blue eyed and willowy, she can act with force or reduce you to the verge of tears. Her voice alone would be worth a fortune in Hollywood."  Johnston starred with Dulcie Gray and Kieron Moore in a psychological melodrama A Man About the House (1947), which was a popular success.
One of Johnston's best known film roles came in the adaptation of Francis Brett Young's novel Portrait of Clare (1950), another box-office success. The following year brought her most high-profile screen appearance, as Robert Donat's long-suffering second wife in the star-studded The Magic Box (1951), made as a project of the Festival of Britain. Johnston was very complimentary about the René Clément-directed Knave of Hearts (1954), a Franco-British co-production of which she said: "The director was brilliant...it was a very sophisticated European film."  Less felicitous was Ealing's Touch and Go (US: The Light Touch, 1955), a farce in which – somewhat ironically in Johnston's case – she and Jack Hawkins played an English couple making plans to emigrate to Australia. Despite early enthusiasm for the venture from the Australian press in particular ( The Age wrote: "For years this versatile and exquisitely beautiful Australian actress...has been wasted...playing dowdy middle-aged frumps. This role may well take her right to the top...at long last.)"  the finished product turned out to be a dud, a failure both with critics and filmgoers, and was Johnston's last screen appearance for several years.
Johnston returned to the screen via the unexpected medium of the horror film Night of the Eagle (1962). It was a successful film, but was not to Johnston's personal taste. She later stated: "I hated that. It was popular, but it wasn't the sort of film I enjoyed doing."  She then appeared in Girl in the Headlines (1963) and Life at the Top (1965), which was more to her liking. ("I did enjoy that. My character was a slut...who wore terrible, loose, awful suits and had a lot of dogs around her.")  Her penultimate screen appearance was in a detective drama The Psychopath (1966) and her final acting role in any medium was Sebastian (1968), another role she said she disliked, which she had only accepted as a favour to producer Michael Powell.
In tandem with her stage and screen appearances, Johnston also played in eight television productions between 1951 and 1964. These were all one-off productions for BBC and ITV drama strands, featuring works by such as Shakespeare, George Bernard Shaw, Noël Coward and Jean Cocteau.
In 1946, Johnston had married Albert Parker, an American film director who had moved to Britain in the early 1930s and had directed several British films before establishing a very successful theatrical agency business (Al Parker Ltd.) in London. Parker was 27 years older than Johnston, and by the mid 1960s was in declining health. From 1965, Johnston was effectively running the business herself and decided, following her appearance in Sebastian, to give up her acting career in order to manage the agency full-time. Following Parker's death in August 1974, Johnston continued to run the business for over 20 years, handling clients such as James Mason, Helen Mirren and Frank Finlay, until her own health started to fail.
The marriage between Johnston and Parker was childless. Johnston died in a nursing home in Kingston upon Thames, London on 19 June 2002, aged 87.
During her professional career, Johnston always gave her year of birth as 1918. Although this would have made her only 18 years old when she moved to Britain – and therefore too young to have studied at the University of Sydney before her departure from Australia – contemporary accounts during her acting career recorded her as four years younger than her actual age, and the 1918 birthdate was also erroneously quoted in some obituaries which gave her age on death as 83.
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