You don’t have to travel outside of the United States to get culture shock.
I got it just last week on a trip to NYC.
As a California native, and as someone who has spent the last few years of my life in Washington state, I’m used to a rather laid-back, polite, easy-going lifestyle. And if that is what you’re used to, then New York is a city that will wake you up to a whole new world.
I was there for four days for a radio conference and awards show. And while there I got to meet other college student pursuing broadcasting careers much in the same way that I am. And it was pleasant. But beyond the confines of our confrence lay a whole world, filled with millions of people who didn’t care if they bumped into you, didn’t exchange pleasantries and were intensely focused on not making eye contact with any strangers.
After my third day of wandering around the Big Apple, I began to question what made this city so different from where I was from. Why did the people seem so disinterested in being polite, and for goodness sake why did everyone seem to always be running into each other?
I was lucky enough to be on a subway this past Saturday morning and in my effort to continue my west coast kindness, apologized to a man for blocking his view of the subway map. He looked at me, almost puzzled by my words and asked me where I was from. I said I was from Washington state and he told me that he was from the midwest. We got to talking and I asked him about the differences he had noticed since living in NYC. He explained to me that the people on the East coast weren’t really rude, and had no intention of coming off that way, but to put it simply everyone was to busy or to focused on where they were going that they didn’t have a moment to stop and say hello, or say “thanks” and “you’re welcome.”
And in that moment it sort of dawned on me that the high pace lifestyle it takes to survive in New York isn’t like Seattle, or Portland, or even Los Angeles. It’s so incredibly different that the people who live there are so hyper focused on their own lives that they stop taking the time to be pleasant to the world around them.
People in New York City aren’t rude, and it’s not my intention to say that they are. But they’re blunt, up front, and determined to use as little words as possible to communicate their message. But it’s because they’re busy. Life is fleeting and politeness is a luxury that those of us from the West coast take for granted.
My simple “thank you” and “please” threw people off in New York because they were no longer accustomed to it. And so the culture shock of New York is real, being on the East coast is like being in another country with people who speak the same language as you, talk like you, but don’t express themselves in the same way. And at the end of the day, if you plan to travel there, be ready for your pleasantries to be ignored. Not because you did anything wrong, but because those things aren’t expected.
When someone hands you your meal, don’t be surprised when your “thank you” solicits an eye roll, or when walking through Times Square and you say “excuse me” after running into someone that they just keep on walking. Life there is different, and it certainly takes some getting used to. But once you’re there you understand the Empire State way and realize that what you may perceive as rudeness, is just everyday life in the Big Apple.


text:
Pretty much a humor blog mixed with some other things I just like. Feel Free to Follow Me and ask me stuff.
I can’t sleep so might as well
I prefer people to spell my name correctly.
I don’t care what other queens do as long as they are happy.
I just don’t know how to deal with it. Like blatantly obvious crushing makes me squirm.
People getting crushes on me makes me physically uncomfortable.

domain: psychology


text:
Some scumbags broke into the Albany Humane Society and stole three puppies, who have since been returned, according to WALB.
Following a break-in at the Albany Humane Society on March 19, three stolen puppies have been safely returned.
According to the Albany Police Department, the puppies were returned after someone called the humane society stating they “wondered in their yard.” WALB is currently unsure if the individuals who returned the dogs received the reward money.
As of now, no one has been arrested for the incident, but they do have a person of interest, 30-year-old Darius Parker.
The front door of the humane society is still in need of repair. After getting a quote for $1,000, and with the shelter being a non-profit, they’re asking for help from a glass company here in Dougherty County, or surrounding counties.

domain: news


Jed Brian (Director, Writer Unlisted Owner) Bel Deliá (Actress, Producer Scratch) Helen Shang (Writer Scratch) Join Exclusive Interview Segment
Jed Brian, Director, and Writer for Unlisted Owner joins this week’s podcast. Brian discusses his adventure in getting for film distribution as well as behind the scene aspects of his lasted film project.
Bel Deliá, Actress, Producer for the award-winning Thriller short, Scratch also joins the exclusive interview segment. Screenwriter Helen Shang joins in a separate interview our coverage of the film, Scratch.
To listen to the latest DecayMag Podcast on Google Home or Google Mini use command; “Ok Google, listen to DecayMag Podcast”
Select your preferred listening, download, sharing audio experience below
My name is Ken Artuz, DecayMag Founder. This week I feature the latest news in Horror cinema. In DecayMag Podcast season 6 episode 10 we’ll be covering the following highlights;
Hellraiser Judgement gets release date (04:21)
Tremors 6 Updates (09:53)
Halloween Cast Update (15:05)
Exclusive Interview Jed Brian (22:25)
On Wednesday, December 20th, 2017 I had the pleasure to speak with filmmaker Jed Brian.
The topic of conversation centered on the drive and business aspect for his full feature
release Unlisted Owner now available on Amazon.
The synopsis to Unlisted Owner reads as follows:
The ‘Owner House’ has been vacant for several years because of its very dark history but
with the recent series of murders it has been taken to the next level. A family who just
moved in has been murdered causing the curiosity of a group of friends to get the best of
them. Deciding to break in and investigate with hand held cameras, would be the worst
decision of their fun filled night. The terrifying and suspenseful footage has been edited for
the film ‘Unlisted Owner’.
Unlisted Owner is a found footage film and although it is one of my least favorite genres of
horror. I find the passion for the project to be fascinating and inspiring.
Below is an excerpt from the official press release
Unlisted Owner, was written and directed by Illinois Native Jed Brian. Starring Gavin Groves,
Haidee Corona, Andrea Potts, Jed Brian, Tyler Landers and Levi Atkins. The film was
released on DVD and through Amazon Streaming on Amazon November 14, 2017.
The movie was filmed entirely in Southeastern Illinois town of Sumner by Jed Brian’s
production company Lawford County Productions. Having a cast mostly from the Sumner
area and actress Haidee Corona from Shelbyville, IN. the film has a strong Midwestern feel.
The film is being released by Tomcat Films/ Summerhill Films.
Unlisted Owner has had a powerful opening on Amazon with pre-orders since launching
eleven weeks ago. The film has had stiff competition competing against… “IT” and “American Horror Story Season 6” to new film releases
such as “47 Meters Down” and “Jeepers Creepers 3″.
“It is so amazing to see Unlisted Owner do so well competing against such well known new releases in the toughest time of competition for a horror film.”
Jed Brian Unlisted Owner Writer/Director
Resources from this segment
Question of the Week
Exclusive Interview Bel Deliá (72:34)
Exclusive Interview Helen Shang (110:05)
On Wednesday, December 20th, 2017 and Friday, December 22nd, 2017 I had the opportunity to
speak with Actress, Producer Bel Deliá, and screenwriter Helen Shang. Both Deliá and Shang were part of the award-winning horror thriller, Scratch. The film won the award for Thriller short at
this year’s Women in Horror Film Festival.
In my interview with Bel Deliá we scratched the surface (pun intended) on the character Deliá portrayed in the film Scratch. Also discussed is the psychological expression of the film and of course
women’s contribution to horror over the years.
In the second interview, Helen Shang went in depth on her period set psychological thriller. The invocation
and reexamination of a suppressed female model under a dominant male antagonist are portrayed in the film Scratch. Sang and I without offering spoilers tackle the issues of the film, Scratch.
Scratch had success in the film festival circuit this year and will release in 2018 for the general
public. No information is offered for the release. Stay tuned to DecayMag for additional
Botlike: A Robots Rampage (150:14)
Hanako: Honor & Blade (153:23)
Trailer First Impressions
Winchester: The House That Ghosts Built (161:54)


OC Sheriff’s Dept respond to burglary in Vidor
On Sept. 1, at 3:31 a.m., the Orange County Sheriff’s Office received a 9-1-1 call reporting a burglary in progress at the Super Stop convenient store located at 2590 North Hwy 105 in Vidor.
An on duty Orange County Sheriff’s Office Detective was near the location of the burglary and responded.
The Detective located the subject on scene and attempted to facilitate a lawful arrest when the subject became combative.
As the Detective was subduing the subject and completing the lawful arrest, a firearm was discharged. Both the Officer and the Subject have been seen by medical staff and released.
The subject has been transported to the Orange County Correctional Facility. The Texas Rangers, along with the Orange County District Attorney’s Office and Sheriff’s Office are currently conducting an investigation and are still on scene.
Further information will be released on this investigation as it becomes available.


text:
How parents handle divorce has an impact on how their children handle it. This comprehensive kit, developed by attorneys who have helped many families through divorce, includes a parents’ book, kids’ book, communication pad, calendar, and stickers – all designed to help families communicate effectively. The 10″ x 13″ box fits on a bookshelf. Each product may also be purchased separately. Includes the parents book, kids’ book, communication pad with mailing envelopes, calendar, and stickers, all in a shrink-wrapped box. For ages 12 and under. 2001. $49.95. IFS
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domain: library


Ballistic shields are mostly used by people in law enforcement. It is that piece of metal that they carry around when in action (eg pacifying rallies or negotiating with kidnappers or robbers). To successfully use a ballistic shield, you should be able to carry it around to protect you effectively while at the same time be able to use your gun efficiently (if needed).
The question of ‘can a ballistic shield protect you?’ really depends on a lot of factors. The first one is training. How trained are you in using your ballistic shield? Can you move around freely while carrying it with you? Can you maneuver your gun and movements while holding this piece of metal to shield your body from potential gun bullets?
When choosing a ballistic shield, you should consider the following:
- Design – do you prefer the traditional rectangular shield that can cover your entire body?
- Weight – how much weight can you carry without affecting your movement
- Ballistic shield test result – how much power it can shield you from. Can it stop a 9mm from reaching you or a .45 caliber from hitting you?
- The handle – the handle should offer balance when you are carrying it, remember that carrying something for a period of time can cause stress fatigue. Carry a shield that you can carry without stressing a part of your arm
- Maintenance – how long can you use a ballistic shield? The manufacturer will tell you the length of effectiveness of a specific shield
Importance of Ballistic Shield Test Result
This is one of the most important factors in choosing a shield. You should know what it can protect you from and what it cannot. A shield test will tell you if your shield is enough to keep you safe from a robber holding a 9mm or not. A ballistic shield test will also give you an estimated distance for safety. Most shields are safe from a 15 feet distance with no penetration.
When ordering a ballistic shield, always ask for a ballistic test so you can see with your own eyes if the shield is penetrable or not. A ballistic shield test will completely eliminate your doubt when it comes to the protection that a shield can provide.


Reduces Pull Weight To Improve Accuracy
Fully machined from A2 tool steel with the hammer notch individually, surface ground to ensure an exact angle of engagement. Reduces felt trigger pull by up to 50 percent. Drop-in installation; use in an unaltered, factory triggerguard. Fits both the .22LR and .22WMR.
Big Book Catalog, Issue:72, Page:166
SPECS: A2 tool steel, in the white. Hardened to Rc 58.


text:
- Research article
- Open Access
The R2R3-MYB transcription factor PaMYB10 is involved in anthocyanin biosynthesis in apricots and determines red blushed skin
BMC Plant Biology volume 19, Article number: 287 (2019)
The majority of apricot (Prunus armeniaca L.) cultivars display orange or yellow background skin, whereas some cultivars are particularly preferred by consumers because of their red blushed skin on the background.
In this study, two blushed (‘Jianali’ and ‘Hongyu’) and two nonblushed (‘Baixing’ and ‘Luntaixiaobaixing’) cultivars were used to investigate the formation mechanism of blushed skin in apricots. High-performance liquid chromatography (HPLC) analysis showed that the blushed cultivars accumulated higher cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside and peonidin-3-O-rutinoside levels during fruit ripening than the nonblushed cultivars. Based on coexpression network analysis (WGCNA), a putative anthocyanin-related R2R3-MYB, PaMYB10, and seven structural genes were identified from transcriptome data. The phylogenetic analysis indicated that PaMYB10 clustered in the anthocyanin-related MYB clade. Sequence alignments revealed that PaMYB10 contained a bHLH-interaction motif ([DE]Lx2[RK]x3Lx6Lx3R) and an ANDV motif. Subcellular localization analysis showed that PaMYB10 was a nuclear protein. Real-time qRT-PCR analysis demonstrated that the transcript levels of PaMYB10 and seven genes responsible for anthocyanin synthesis were significantly higher in blushed than in nonblushed apricots, which was consistent with the accumulation of anthocyanin. In addition, bagging significantly inhibited the transcript levels of PaMYB10 and the structural genes in ‘Jianali’ and blocked the red coloration and anthocyanin accumulation. Transient PaMYB10 overexpression in ‘Luntaixiaobaixing’ fruits resulted in the red blushed skin at the maturation stage.
Taken together, these data reveal that three anthocyanins are responsible for the blushed skin of apricots, identify PaMYB10 as a positive regulator of anthocyanin biosynthesis in apricots, and demonstrate that blush formation depends on light.
Apricot (Prunus armeniaca L.) is a widely cultivated temperate fruit tree species  with fruit that contains many types of carotenoids and presents with orange or yellow skin. Xinjiang is one of the primary centers of apricot domestication worldwide and is also the main cultivation area in China; almost 200 varieties are cultivated in this region . Among these varieties, some cultivars with a red blush on the orange or yellow background skin are particularly preferred by consumers due to their beautiful color and high nutritional value [3, 4]. However, the mechanism underlying blush formation in these cultivars is unknown.
Anthocyanins are water-soluble flavonoid pigments that accumulate in the vacuoles of many flowers, fruits, seeds, and vegetables, where they contribute to the red, purple and blue coloration . As the primary color determinant and antioxidant components, the accumulation of anthocyanin pigments in fruit is an important indicator of health-promoting property [6, 7]. Therefore, understanding the regulation of anthocyanin biosynthesis in fruits is important for development of anthocyanin-rich foods for our diet.
Anthocyanins are synthesized from a branch of the flavonoid pathway, which has been extensively studied in many plants [8,9,10]. In general, anthocyanin biosynthesis at the transcriptional level is controlled by a complex of DNA-binding R2R3 MYB transcription factors, MYC-like basic helix–loop–helix (bHLH) proteins and WD40 proteins [11,12,13]. MYB members of this complex can separately play the regulatory role in anthocyanin biosynthesis [14, 15], and have been identified in various fruit crops, including grapes , apples , pears  and peaches .
MYB TFs have been reported to have diverse functions in controlling plant pathways, such as secondary metabolism, development, signal transduction, and disease resistance . These TFs are characterized by a structurally conserved DNA-binding domain consisting of single or multiple imperfect repeats. Among these MYB TFs, the two repeat class (R2R3) is the largest, with 133 R2R3 MYB TFs reported in Arabidopsis [21, 22] and 108 reported in grapes . It has been clearly elucidated that R2R3 MYB TFs are the key to determine the spatial and temporal patterning of anthocyanin production in most plant species [7, 15, 24]. Hence, the isolation and characterization of MYB TFs associated with anthocyanin biosynthesis is an important step towards understanding and manipulating fruit coloration .
Recently, great progress has been made in elucidating the molecular control of the anthocyanin pathway in many horticulture crops . Anthocyanin biosynthesis is enhanced by sunlight in apples, pears, and peaches . In apricots, although blushed skin is observed in some cultivars, no information about the mechanism of its development is available at present. In the present study, we determined the main pigments contributing to fruit blushed skin and identified one R2R3 MYB transcription factor and seven structural genes of the anthocyanin biosynthetic pathway based on coexpression network analysis (WGCNA). The potential roles of MYB in anthocyanin accumulation in apricots were investigated by bagging and transient overexpression. Our results suggest that PaMYB10 is responsible for controlling anthocyanin biosynthesis and is involved in the development of blushed skin in apricots and that the process is regulated by light.
Changes in basic quality parameters during fruit development and ripening
From the fruitlet (F) to fully ripe (FR) stages, the fruit weight of all experimental cultivars increased rapidly and presented a typical S-shaped curve (Fig. 1a), whereas the fruit firmness declined sharply throughout the development period (Fig. 1b). The TSS concentration in all fruits increased remarkably with fruit development (Fig. 1c) and the TA concentration decreased significantly (Fig. 1d), which showed that the five developmental stages completely covered the full process of fruit development and ripening. The four apricot cultivars showed a gradual color change from the turning (T) stage. The a* value increased during ripening and was positive at the enlargement (E), commercial maturation (CM) and FR stages in ‘HY’ and ‘JNL’, indicating that the fruit was turning red, whereas the value was negative during the whole ripening process in ‘LT’ and ‘BX’ (Additional file 1: Table S1). At the CM stage, a blush appeared on the fruit skin of ‘JNL’ and ‘HY’, whereas the skins of ‘LT’ and ‘BX’ did not display blush (Fig. 2a). Generally, the color index for red grape (CIRG) is used to evaluate the redness of fruit. The CIRG values of all fruit peels increased with development, although the CIRG values of the ‘HY’ and ‘JNL’ fruit peels were significantly higher than those of ‘BX’ and ‘LT’ (Fig. 2b).
Changes in the anthocyanin content during fruit development and ripening
Three anthocyanins (cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside and peonidin-3-O-rutinoside) were identified from the apricot peels (Fig. 3). During development and ripening, the anthocyanin contents of ‘BX’ and ‘LT’ remained very low, whereas those of ‘JNL’ and ‘HY’ increased significantly. For ripe fruit, the total anthocyanin concentrations in ‘JNL’ and ‘HY’ were 35.05 mg/kg·FW and 39.17 mg/kg·FW, respectively, which were significantly higher than those in ‘BX’ (0.09 mg/kg·FW) and ‘LT’ (0.07 mg/kg·FW). Interestingly, we detected cyanidin-3-O-rutinoside in the red cultivars; it accounted for 58.8% and 62.7% of the total anthocyanins in ‘JNL’ and ‘HY’, respectively, but was not detected in the ‘BX’ and ‘LT’ fruit peels.
Screening of candidate genes by WGCNA
We performed a WGCNA to investigate the transcription factors and structural genes responsible for anthocyanin biosynthesis. We found that the blue and green modules were significantly positively correlated with cyanidin-3-O-glucoside, with correlation coefficients of 0.92 (e-value = 4 × 10− 4) and 0.94 (e-value = 1 × 10− 4), respectively. Similar significant correlations were found between the two modules and cyanidin-3-O-rutinoside and peonidin-3-O-rutinoside (Fig. 4). Based on the correlation coefficients between genes in the blue and green modules and the anthocyanin compounds, and changes in Fragments Per Kilobase of transcript per Million fragments mapped (FPKM) values of these genes during fruit ripening (Fig. 5), one U1373 gene encoding MYB was located in the blue module, and seven structural genes were identified as putative candidate genes for anthocyanin biosynthesis in apricots, including one chalcone isomerase (CHI: U21320) and one UDP-glucose: flavonoid 3-O-glucosyltransferase (UFGT: U3633) located in the blue module and one phenylalanine ammonia lyase (PAL: U15872), one chalcone synthase (CHS: CL2328.1), one flavonoid 3-hydroxylase (F3H: CL472.1), one dihydroflavonol 4-reductase (DFR: U22536), and one leucoanthocyanidin dioxygenase (LDOX: U21017) located in the green module (Additional file 2: Table S2).
Phylogenetic and conserved motif analyses and subcellular localization of PaMYB10
A bootstrapped circular phylogenetic tree was constructed based on 148 MYB genes from the apricot transcriptome and MYB transcription factors in other species. The tree showed that the 148 MYB TFs were distributed into five clades (Additional file 3: Figure S1), including flavonoid-related MYB, water stress-related MYB, phenol-related MYB, cell wall composition-related MYB, and some unknown function MYB clades. U1373 was clearly clustered with other MYB transcription factors involved in flavonoid synthesis. To further verify the results, another phylogenetic tree was generated based on U1373 and 18 flavonoid-related MYB transcription factors from other species. The second phylogenetic tree showed that U1373 belonged to the anthocyanin-related MYB clade and clearly clustered with PpMYB10, showing high homology with PbMYB10 in pear (Pyrus bretschneideri) fruit and MdMYB1 and MdMYB10 in apples (Malus domestica) (Fig. 6a) [27, 28]. Therefore, U1373 was designated PaMYB10.
Furthermore, the full-length U1373 was isolated from the peel of ‘JNL’ using RACE. The sequence was 675 bp in length and contained a complete open reading frame encoding 225 amino acids. Subsequently, comparison of the deduced amino acid sequence of PaMYB10 with those of several anthocyanin-related MYB transcription factors from other plants revealed a high degree of sequence similarity in the R2 and R3 DNA-binding domains of these proteins. As shown in Fig. 6b, a conserved KPRPR [S/T] F motif was also present in the R2R3 domain of the PaMYB10 C-terminus. Further analysis showed that the R3 DNA-binding domain contained an R/B-like bHLH interaction motif ([DE]Lx2[RK]x3Lx6Lx3R) and an ANDV motif.
To determine the subcellular localization of PaMYB10, tobacco (Nicotiana tabacum) leaves bombarded with the recombinant vector pC1301-GFP-MYB10 and control vector pC1301-GFP were observed under a confocal laser scanning microscope. We found that the tobacco leaves with the control vector pC1301-GFP exhibited fluorescence throughout the entire cell, whereas those with the recombinant vector p163-GFP-MYB10 displayed strong fluorescence in the nucleus. These results demonstrated that the expression site of this gene was in the nucleus (Fig. 6c).
Transcript levels of PaMYB10 and structural genes related to anthocyanin synthesis
The results showed that the transcript levels of all genes in the peels of the blushed cultivars (‘JNL’ and ‘HY’) were significantly higher than those in the peels of the nonblushed cultivars (‘BX’ and ‘LT’) (P < 0.05) (Fig. 7). The transcript levels of PaMYB10, PaPAL, PaCHS, PaCHI, PaF3H, PaDFR, PaLDOX and PaUFGT were low in ‘BX’ and ‘LT’, with only slight upregulation observed in the F and T stages. However, the expression levels of PaMYB10 and the seven structural genes in ‘JNL’ and ‘HY’ all increased remarkably during the whole developmental period, whereas the expression patterns of PaMYB10 and most of the structural genes in ‘HY’ showed a double-peak pattern and reached their highest values in the CM stage.
The effect of bagging on gene expression and anthocyanin accumulation in the ‘JNL’ fruit
The a* value in bagged fruits (5.87) was significantly lower than that in nonbagged fruits (20.84) (Additional file 1: Table S1), and the CIRG values also decreased from 1.20 to 0.90. No blush was observed in the bagged ‘JNL’ fruit skin (Fig. 8a). The cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside and peonidin-3-O-rutinoside contents in bagged fruits were extremely reduced from 35.05 mg/kg FW to 2.17 mg/kg FW in the ‘JNL’ peel (Fig. 8b), and the PaMYB10, PaPAL, PaCHS, PaCHI, PaF3H, PaDFR, PaLDOX and PaUFGT expression levels were also significantly lower than those in the nonbagged fruits (P < 0.05) (Fig. 8c).
Transient PaMYB10 overexpression increases anthocyanin production in the ‘LT’ fruit peel
PaMYB10 was transiently overexpressed in the ‘LT’ fruit peel at the CM stage. The fruit peel presented with a red blush, whereas the control fruit skin did not present with a blush (Fig. 9a). Compared with those of the control, the cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside and peonidin-3-O-rutinoside contents were significantly increased by 2.2-fold, 3-fold and 2.4-fold, respectively, in the PaMYB10-overexpressed fruits (Fig. 9b).
Apricots with a blush on orange or yellow skin are becoming more and more popular in the market due to their colorful appearance and excellent nutritional value ; however, the mechanism underlying the development of these cultivars has remained elusive. In this study, we compared the anthocyanin composition and content among apricot cultivars with and without a blush. Based on the WGCNA, gene expression and gene structural analyses and transgenic experiments, we identified the R2R3-MYB TF PaMYB10 as a master regulator that controlled the entire anthocyanin biosynthesis process during apricot fruit ripening.
Our chemical analysis demonstrated that the total anthocyanin content in apricots with red blushed skin as well as the individual cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside and peonidin-3-O-rutinoside contents were significantly higher than those found in apricots without a blush (Fig. 3). In particular, remarkably high cyanidin-3-O-rutinoside levels were observed in red blushed cultivars during fruit ripening but were undetectable throughout the whole ripening process in cultivars without a blush. These results showed that high levels of the three anthocyanins determined the blush coloration of the apricot fruit skin and that cyanidin-3-O-rutinoside played the dominant role in blush formation. As shown in Fig. 2a, the ‘JNL’ and ‘HY’ fruit skins turned red after the T stage, which showed that the blush depended on fruit ripening. Thus, to understand the molecular mechanism of blush formation, the T, CM and FR stages of ‘JNL’ fruits, which represented the full ripening process, were used for transcriptome sequencing. Using WGCNA, we identified one MYB gene and seven structural genes related to anthocyanin biosynthesis in the blue and green modules that were significantly positively correlated with cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside and peonidin-3-O-rutinoside (Fig. 4). MYB was considered the hub gene in the anthocyanin metabolic network, which suggested a direct connection between MYB and anthocyanin biosynthesis in apricot fruit.
For functional studies, full-length PaMYB10 cDNA was cloned from ‘JNL’. In the subsequent phylogenetic analysis, PaMYB10 was found to cluster with PpMYB10, PbMYB10, MdMYB1 and MdMYB10 (Fig. 6a), which are known to be positively associated with anthocyanin production [27, 28, 30, 31]. Sequence alignment found that the apricot PaMYB10 contained the typical R2 and R3 structural domains similar to other MYB members in the same cluster (Fig. 6a). Sequence analysis also showed that PaMYB10 contained an ANDV motif in the R3 domain, which is an identifier for anthocyanin-related MYBs . In addition to the R2R3 domain, the KPRPR [S/T] F motif, which is a R2R3-MYB specific structure , was found in PaMYB10. Finally, the subcellular localization analysis revealed that PaMYB10-GFP was distributed in the nucleus (Fig. 6c). These results suggested that PaMYB10 may play an important role in regulating anthocyanin synthesis in apricot fruit.
We also compared the expression levels of PaMYB10 and structural genes related to anthocyanin biosynthesis between blushed and nonblushed apricots and found that the transcript levels of all genes in the apricots with blush were significantly higher than those in the apricots without blush during the fruit ripening process. These findings were consistent with the accumulation of anthocyanins, which proved that these genes were related to anthocyanin biosynthesis (Fig. 7). At the same time, we demonstrated that bagging resulted in a significant decrease in the accumulation of anthocyanins in ‘JNL’ and prevented blush formation in the fruit skin (Fig. 8b), which coincided with a dramatic decrease in the transcript levels of PaMYB10 and the structural genes (Fig. 8c). These results further confirmed that PaMYB10 was a key gene involved in the control of anthocyanin biosynthesis and simultaneously suggested that light could affect blush formation on apricots. These findings are in agreement with previous reports in other fruits linking the light-mediated mechanism involved in the regulation of anthocyanin biosynthesis with ripening-related anthocyanin accumulation .
In plants, transcriptional regulation of structural genes by transcription factors appears to be the major mechanism controlling anthocyanin biosynthesis [14, 32, 33]. Most MYBs involved in the control of anthocyanin biosynthesis are positive regulators that enhance the expression of structural anthocyanin pathway genes. However, repressors have also been characterized, such as VvMYB4 in the berries of grapevines and FaMYB1 in strawberries (Fragaria x ananassa Duch.) [23, 34]. In the present study, transient overexpression of a single PaMYB10 gene in ‘LT’ apricots promoted blush formation and significantly increased the transcript abundance of PaMYB10 and structural genes responsible for anthocyanin biosynthesis. These results suggested a central positive role for PaMYB10 in regulation of anthocyanin synthesis.
R2R3 MYB TF regulation can occur at different steps of the anthocyanin biosynthesis pathway. For example, R2R3 MYB TFs in perilla (Perilla frutescens) control transcription of all structural genes involved in anthocyanin biosynthesis . Overexpression of AtPAP1 and AtPAP2 strongly induce anthocyanin accumulation in Arabidopsis as a result of upregulation of all genes in the anthocyanin biosynthetic pathway [36, 37]. Similarly, our results showed that almost all genes responsible for anthocyanin biosynthesis were up-regulated in overexpressed apricot fruit of PaMYB10, which suggested the global regulation role of PaMYB10 for the whole biosynthesis pathway of anthocyanin biosynthesis in apricot, but future work is still required to investigate which genes are targeted by the MYB TF.
In general, regulation of the anthocyanin biosynthesis pathway is controlled by the MYB-bHLH-WD40 (MBW) complex [7, 23]. But some anthocyanin biosynthesis-related MYB transcription factors that are activated without a bHLH partner have been identified in grapevines and Arabidopsis thaliana [24, 38]. In the present study, the amino acid signature ([DE]Lx2[RK]x3Lx6Lx3R) of the R3 domain, which was used to connect the MYB and bHLH proteins , was found in the PaMYB10 protein, suggesting a potential interaction between the bHLH protein and PaMYB (Fig. 6b), so more evidence should be provided for whether other TFs may be coordinately involved in the process.
In the study, we found that the red blushed skin of apricots is attributed to the accumulations of cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside and peonidin-3-O-rutinoside during fruit ripening. The R2R3 MYB TF PaMYB10 was identified as a central gene related to the regulation of anthocyanin biosynthesis and blush formation, and the process is regulated by light.
Four varieties of apricot [‘Jianali’ (JNL) and ‘Hongyu’ (HY) (fruit with blushed skin) and ‘Baixing’ (BX) and ‘Luntaixiaobaixing’ (LT) (fruit without blushed skin)] cultivated at the National Fruit Tree Germplasm Repository, Academy of Xinjiang Agricultural Sciences, Luntai, Xingjiang, China, were used as the experimental materials. Every six individual trees were treated as one replication, and fruits were harvested at the fruitlet (F, 30 DAB), enlargement (E, 45 DAB for ‘BX’, ‘LT’, ‘JNL’ and ‘HY’), turning (T, 60 DAB for ‘BX’ and ‘LT’ and 54 DAB for ‘JNL’ and ‘HY’), commercial maturation (CM, 75 DAB for ‘BX’ and ‘LT’ and 72 DAB for ‘JNL’ and ‘HY’) and fully ripe (FR, 90 DAB for ‘BX’ and ‘LT’ and 82 DAB for ‘JNL’ and ‘HY’) stages. In addition, fifty ‘JNL’ fruits were bagged before the T stage and harvested at the FR stage. Twenty of the fifty harvested fruits were used to measure basic physiological indexes, such as the fruit size, chromatic aberration, total soluble solids (TSS), and titratable acidity (TA). Three biological replicates for each sample were used. The peel and pulp were separated from the remaining 30 sampled fruits, immediately frozen in liquid nitrogen, and stored at − 80 °C prior to use.
Anthocyanin extraction and identification
Total anthocyanins were extracted from the fruit peels according to the methods described by our previous study with slight modifications . Frozen peels (1 g) were ground to a fine powder by an IKA A11 analytical grinder (IKA, Staufen, Baden-Württemberg, Germany) and extracted using 3 mL of 2% formic acid for 24 h at 4 °C in the dark. The extraction was evaporated using a rotary evaporator for the next step. The leachate residue was dissolved in methanolic hydrochloric acid solution (pH = 2.3) and then adjusted to a final volume of 1.5 mL. Subsequently, the clear supernatant was collected and filtered to remove cell debris. Anthocyanins in the samples were analyzed using a Waters HPLC system with a diode array detector (Waters, Milford, MA, USA) at 40 °C. Each sample (20 μL) was loaded onto a reversed-phase C18 analytical column (4.6 mm i.d. × 250 mm i.d., 5 μm) (Nacalai Tesque, Kyoto, Japan) and eluted using a mobile phase consisting of solvent A (2% formic acid in water) and solvent B (2% formic acid and 98% acetonitrile) for 40 min at a flow rate of 1 mL per min. The effluent absorbance was monitored at 525 nm using the Genesys 10 UV spectrophotometer (Thermo Spectronic, Rochester, NY, USA). The concentration was calculated by the standard curve method and expressed as mg/kg·FW. Three biological replicates for each sample were used.
RNA extraction and cDNA synthesis
Total peel RNA was extracted from the fruit using a total RNA kit (RNeasy Plant Mini Kit, Qiagen, Hilden, Germany). RNA samples with an OD260:OD280 between 1.80 and 2.0, an OD260:OD230 > 1.5 and no discernible degradation were used for cDNA reverse transcription. The RNA was treated with the TURBO DNA-free™ Kit (Ambion, Austin, TX, USA) following the manufacturer’s instructions. Subsequently, first-strand cDNA was synthesized from 1 μg of total RNA using the RevertAid First Strand cDNA Synthesis Kit (Invitrogen, Tokyo, Japan) according to the manufacturer’s protocol.
Transcriptome sequencing and weighted gene coexpression network analysis (WGCNA)
‘JNL’ peel transcriptome sequencing was conducted on the Illumina HiSeq2000 (Illumina, USA) platform. Sequence assembly and annotation were performed according to our previous work . The WGCNA (v1.29) package in R was employed to construct coexpression networks and test the association with the anthocyanins. Sequences with an average NRPKM > 1 from three replicates were used for the WGCNA. The modules were obtained using the automatic network construction function blockwise with default settings, except that the soft power was 16, the min module size was 30, and the merge cut height was 0.25.
A total of 148 MYB sequence fragments were mined from our transcriptome data, and a phylogenetic tree was constructed with MYB transcription factors from other species. After homology alignment, another unrooted phylogenetic tree was constructed using the neighbor-joining (NJ) criteria and verified using the maximum likelihood (ML) method. A total of 1000 bootstrap replicates was performed based on multiple alignments of amino acid (AA) sequence U1373 from JNL and 18 flavonoid-related MYB transcription factors from other higher plants (Additional file 4: Table S3). Multiple protein sequence alignments were performed using ClustalW, and the phylogenetic trees were constructed using MEGA 7.0.
Quantitative real-time PCR analysis
Eight pairs of primers (Additional file 5 Table S5) were designed using Primer Premier 5.0, and qRT-PCR was performed using the Light Cycler System (Roche LightCycler® 480; Roche Diagnostics). All reactions were performed using the LightCycler® 480 SYBR Green I Master Mix (Roche Diagnostics) according to the procedure described by the manufacturer. The qRT-PCR program was 95 °C for 30 s, followed by 40 cycles at 95 °C for 5 s and 60 °C for 30 s. The reaction volume was 25 μL and contained 12.5 μL of SYBR Premix Ex Taq (TaKaRa, Dalian, China), 8.5 μL of deionized water, 1 μL of each primer, and 2 μL of diluted cDNA. Three biological replicates for each sample were used. Each sample was normalized using ribosomal RNA and actin as the internal control genes [39, 40]. The relative expression levels of the eight genes were calculated using the 2-△△Ct method.
Cloning of the full-length PaMYB10 gene
The PaMYB10 gene was isolated from the cDNA of ‘JNL’ using a RACE kit (Takara, Dalian, China) (Additional file 6 Table S5). The full-length PaMYB10 was obtained by assembling the 3′ RACE and 5′ RACE sequences using the BigDye Terminator Version 3.1 Cycle Sequencing Kit (Applied Biosystems) with the ABI PRISM 3100 Genetic Analyzer (Applied Biosystems). The amplification product was cloned into the pMD18-T vector and then transformed into Escherichia coli DH5α (BioTeke, China). Afterwards, positive colonies were sequenced by Sangon Biological Engineering Company (Shanghai, China).
The PaMYB10 open reading frame (ORF) without the terminator was amplified with special primers (forward: 5′-GAGAACACGGGGGACTGGTACCCGGGGATCCA TGGAGGGCTATAACTTGGA-3′ and reverse: 5′-ACAGCTCCTCGCCCTTGCTC ACCATGTCGACCCTAGCTTCTTCTGAAACAT-3′) and ligated into the pC1301-GFP vector to generate the CAMV 35S:PaMYB10-GFP fusion construct. The recombinant vector (PaMYB10-GFP) and control vector (pC1301-GFP) were both transformed into tobacco by particle bombardment. After 16 to 18 h, fluorescence was detected with a confocal laser scanning microscope (Zeiss LSM 510 META, Jana, Germany).
Overexpression vector construct and transient transformation in apricot fruit
The overexpression pMDC32 binary vector49 was constructed as previously described [41, 42]. The PaMYB10 coding sequence was PCR amplified from ‘JNL’ cDNA using the primers 5′-ATGGAGGGTTATTTCGGTGTGAG-3′ (forward) and 5′-TACGTAGGAGATGTTGACTAGATCATTGC-3′ (reverse) and cloned using the pCR™8/GW/TOPO TA Cloning Kit (Invitrogen). After sequencing confirmation, the CDS was recombined into the pMDC32 binary vector49 using the Gateway LR Clonase II Enzyme mix (Invitrogen) to produce overexpression vectors. The vectors were transformed into Agrobacterium tumefaciens GV3101 and then resuspended at a final O.D. of 0.8 in liquid MS medium. One milliliter of the suspension was evenly injected into the ‘LT’ fruit at commercial mature stage. Thirty fruits were injected as the repeat, and fruits were injected with Agrobacterium carrying an empty vector as a control treatment. At 7–12 days after injection, the fruit peel surrounding the injection sites was collected and immediately frozen in liquid nitrogen and stored at 80 °C for determining the anthocyanin according to the method of above mentioned.
Three biological replicates were performed for all experiments. The least significant difference (LSD) or Duncan’s test at the 1% level was analyzed by DPS (version 2.00; Zhejiang University, Hangzhou, China). Data are expressed as the mean of three biological replicates ± the standard deviation (SD).
Availability of data and materials
The data sets supporting the results of this article are included within the article and its additional files. All sequences obtained from apricots were deposited to the NCBI Sequence Read Archive (SRA) repository under accession number PRJNA506502 and were released on November 27, 2018.
Least significant difference
UDP-flavonoid glucosyl transferase
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We thank Julie Caruana (Department of Cell Biology and Molecular Genetics, University of Maryland) for comments on an earlier version of this manuscript.
This work was supported by the National Natural Science Foundation of China (31872046). There is on role of the funding bodies in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.
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The authors declare that they have no competing interests.
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Table S1. Color indexes of apricot fruits at different developmental stages. (PDF 251 kb)
Table S2. Candidate genes involved in anthocyanin biosynthesis in apricot fruit. (PDF 214 kb)
Figure S1. Phylogenetic tree of gene sequences for 148 MYB genes from the apricot transcriptome and MYB transcription factors from other species. (PDF 823 kb)
Table S3. Flavonoid-related MYB transcription factors using in phylogenetic analysis. (PDF 227 kb)
Table S4. The primer sequences for qRT-PCR. (PDF 363 kb)
Table S5. The primer sequences for 5′ RACE and 3′ RACE. (PDF 351 kb)
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Cite this article
Xi, W., Feng, J., Liu, Y. et al. The R2R3-MYB transcription factor PaMYB10 is involved in anthocyanin biosynthesis in apricots and determines red blushed skin. BMC Plant Biol 19, 287 (2019). https://doi.org/10.1186/s12870-019-1898-4
- Prunus armeniaca
- MYB transcription factor

domain: literature


Genre: Comedy, Drama
Directed by: Mike White
Starring: Ben Stiller, Austin Abrams, Jenna Fischer, Michael Sheen, Jermaine Clement, Luke Wilson
The movies have never lacked for portrayals of the male mid-life crisis. 8 1/2, Husbands, The Swimmer, Sideways; some of the best films in cinematic history have centred on middle-aged men in existential turmoil. Despite re-treading this decidedly old ground, writer-director Mike White finds plenty to say in his sophomore feature, Brad’s Status.
Brad (Ben Stiller), is the director of a small but successful non-profit, based in California. He’s happily married to Melanie (Jenna Fischer), with whom he has a teenage son, Troy (Austin Abrams). Though on the face of it, his life looks perfect, during the week he takes Troy to visit Harvard, Brad is plagued by insecurities about his place in the world, and how his life has turned out compared to his own wildly successful college classmates (Luke Wilson, Michael Sheen, Jermaine Clement).
The movie is propelled by Brad’s voice-over, and it’s extremely personal; we are privy to his secret desires; we hear each thought that flickers through his fraught and addled mind. These thoughts are always neurotic, and often embarrassingly so, but each one will feel relatable to anyone who has ever worried about dropping behind in life as their friends are surging forward. They may not be pretty thoughts, but they’re honest.
When you talk about the best portrayers of the ‘everyman’ working in Hollywood today, the first name to come up is typically Tom Hanks. Over his long career, and more acutely in recent years with films like The Secret Life Of Walter Mitty, The Meyerowitz Stories and now Brad’s Status, Ben Stiller has proven himself worthy of inheriting that crown.Stiller is so adept at handling men who get stuck within the mire of their own minds (Brad is very much a Walter Mitty type, even down to his daydreams). You can find Brad endlessly frustrating, but Stiller keeps his humanity and vulnerability at the forefront, and so you never stop wishing the best for him. Witnessing his growth over the course of the film is a surprisingly moving experience; that perfectly-pitched final exchange between Brad and Troy earns every emotion it elicits.
It isn’t just the status insecurities that accompany a mid-life crisis that White explores in his witty and astute screenplay. A lot of time is dedicated to tackling the often-cavernous gap between millennials and their parent’s generation. A lengthy dialogue between Brad and Ananya (Shazi Raja), a first-year student at Harvard, is a fascinating swirl of idealism meeting realism; adulation confronted by blunt honesty. Anything new that Brad’s Status has to say about undergoing a mid-life crisis is said during the scenes shared between Brad and Ananya, and they are riveting.
There’s so much going on in Brad’s Status that it would be easy to overlook just how entertaining it is. From Stiller’s many instances of embarrassing his son and his ridiculous daydreams, to exaggerated performances from Jermaine Clement and Michael Sheen, there are few moments that pass without a laugh, or at least a rueful smile. That there’s such a truth behind the funny moments makes them all the funnier.
Brad’s Status may re-tread old ground, but it does so with a spring in its step and a wise head on its shoulders. It’s a sterling screenplay from Mike White, and yet another reminder that we don’t give Ben Stiller enough credit as a dramatic actor.


text:
October’s started off as a great month for stand up comedy in Central PA; local comics and big names alike have been showing at venues from Lancaster to Harrisburg… and it shows no signs of slowing down now. For two shows and one night only, Dave Chappelle is coming to the Santander Performing Arts Center in Reading, tonight, the night of October 6.
Yeah. “Half-Baked” Dave Chappelle. Stand up god Dave Chappelle. Superlative of all things comedy Dave Chappelle. Him. Lest you need a reminder:
Looking for more to do tonight, tomorrow, or any other time? Check out flymagazine.net for all your after-5-o’clock needs. Want to tell the world what you’re doing after work? Hashtag #FlyAfter5 on Twitter or Instagram to let us know.

domain: artistic


Panasonic has introduced a new rugged tablet with a removable battery, which is a rarity in our time.
The tablet is quite thick, which is not surprising given its rugged design. 7-inch display has a resolution of 1280 x 800 and a brightness of 500 cd / m22… The screen supports glove operation, and Panasonic has also added a rain mode that will keep the screen as responsive as the rest of the time.
The tablet is based on the Snapdragon 660 SoC, it has 4GB of RAM and 64GB of flash memory, as well as a memory card slot. There is a built-in barcode scanner, a full-size USB-A port and a USB-C connector. The tablet complies with IP67 protection and the requirements of the American military standard MIL-STD-810H. It can withstand drops to a hard surface from heights of up to 1.5 m.
The tablet has a hot-swappable battery, that is, the device does not turn off, as it contains another small battery. Panasonic will sell replacement batteries in various capacities.
The Panasonic Toughbook S1 comes in Wi-Fi and LTE versions. It runs Android 10 out of the box, but the company will update it to Android 12. This commercial-grade device starts at $ 2,499.
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Musk said the Model 3 sedan was the third product on which Tesla gambled its existence, after its first vehicle, the Roadster sports auto, and the Model S sedan, which represented a sizable increase in annual production.
The $7,500 federal tax credit for electric vehicles is set to start phasing out for the Model S, Model X and Model 3 after Dec.31, according to the company's website. This was followed by an encouraging trend displayed by Bloomberg's Model 3 production tracker, which now forecasts that Tesla would be able to sustain its "burst" production rate of 5,000 vehicles per week for the next three weeks.
Musk had hoped to make vehicle production faster and less expensive by significantly increasing the amount of automation Tesla used in its factories.
Musk also told Bloomberg that at one point he wore the same clothes for five consecutive days, and he compared circumstances at Tesla with those at General Motors, where he said executives used a designated elevator to avoid contact with other employees. But the company struggled to increase automation and production capacity at the same time after launching its Model 3 sedan. Tesla told Bloomberg that it was not aware of such instructions and that the plumbing issue was quickly resolved.
"We thought it would be good, but it was not good".
This recent filing stands as yet another sign that Tesla is well on its way to sustaining its production rate of 5,000 Model 3 per week this third quarter. The Model 3 was created to be Tesla's first mass-market vehicle, meant to broaden the company's customer base beyond the luxury segment and increase the rate of electric-vehicle adoption.
With more than 400,000 customers holding deposits on the Tesla Model 3, many of those customers will likely not receive any federal tax credit.
On July 2, the company said it made 5,031 Model 3s during the final week of June and 28,578 during the second quarter, more than it had made in the prior three quarters combined.


text:
This Large Vintage Artwork is the decor piece you've been missing! It will have you daydreaming about a happy place any time you walk by and bring you a sense of calmness and peace. The colours in this artwork can easily tie into a wide range of spaces and bring in a vintage touch to your home with its amazing frame!
Measures: 32.5"H X 55.75"L X 2.5"W
Wooden frame with hand painted canvas print.
This is a pre-loved item.
Small natural flaw in wooden mat at bottom left.
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domain: design


The first UN-chartered vessel loads grain for export to Ethiopia, in Yuzhne, east of Odessa on the Black Sea coast on August 14, 2022. UNCTAD is endorsing calls to renew the Black Sea Grain Initiative, a provisional agreement involving Russia, Ukraine and Turkey to keep the flow of grain exports.
A United Nations trade agency is endorsing calls to renew the Black Sea Grain Initiative, a provisional agreement involving Russia, Ukraine and Turkey to keep the flow of grain exports even as Moscow and Kyiv continue to fight.
The United Nations Conference on Trade and Development (UNCTAD) says the Initiative, which began in July, to have Ukrainian grain exported via the Black Sea, has offered hope and shown the power of trade in times of crisis.
The UNCTAD says in its latest report that the Initiative, which expires at the end of October should be renewed.
“Port activity in Ukraine is picking up and large shipments of grain are reaching world markets. As of 19 October, the total tonnage of grain and other foodstuffs exported through the initiative had reached almost 8 million metric tonnes,” reads the report.
The UN-led Initiative, brokered by Turkey, has helped to stabilise and subsequently lower global food prices and move precious grain from one of the world’s breadbaskets to the tables of those in need. Ukraine, which has since been invaded by Russia, had been the world’s biggest wheat exporter especially to poor African countries and the Middle East. After the war started, military activity in the waters and around the ports prevented exports, until Ankara brokered the deal last July.
Global food prices
The Food Price Index published by the UN Food and Agriculture Organisation has shown that the prices of global food staples have declined in recent months by about 8.6 per cent in July, 1.9 per cent in August and 1.1 per cent in September as a result of the initiative.
But with the initiative ending in November and its renewal uncertain, the prices of some commodities such as wheat and maize are rising again, the report warns.
“Without the initiative, there is little hope for providing food security, especially in developing and least developed countries,” read the report.
While shipments are still about 40 per cent to 50 per cent, port activities in Ukraine are still below the pre-war period but the trend is in the right direction.
Under the UN-led initiative, three seaports have been reopened – Odesa, Chornomorsk, Pivdennyi/Yuzhny. As a result, the number of port calls has increased and more importantly, the volumes carried have risen significantly.
The report states the weekly tonnage of grain shipped under the initiative reached 1.2 million tonnes by September. This is still below 2021 levels, but the gap is closing.
Respect UN agreements
This week, the Ukrainian government also called on Russia to respect UN agreements to facilitate supply of basic commodities in Africa and other countries globally.
Ukraine Ambassador to Kenya Pravednyk Andrii urged Russia to allow extension of UN’s agreement on Black Sea grain corridor to allow his country to continue supplying grain past November when the agreement comes to an end.
On July 22, Ukraine, Turkey and the UN signed an agreement in Istanbul that concerns the unblocking of three Ukrainian seaports. Russia signed a mirror agreement with Turkey and the UN, providing assurances of safe and secure passage of the ships, which allowed the shipments of Ukrainian grain and foodstuffs via the Black Sea grain corridor until November this year.
Source Article: The East African


Optimal mechanical components were designed using biomechanical modeling and inverse dynamics analysis. The prosthetic knee includes a linear spring and friction dampers that engage and disengage at optimal times in the gait cycle to provide able-bodied kinematics with minimal patient energy expenditure. The spring and damping system provide early stance flexion-extension and late stance and swing control respectively. The design has an automatic early stance lock for stability. The spring stiffness and damper friction may be adjusted for patient and device weight. A small user trial showed a smooth transition from stance to swing and easy usability of the prosthetic. This invention is a passive prosthetic knee design that is low cost with improvements over current devices that could allow large scale manufacturing for widespread use in developing countries.


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Axel Back for Beverly Hills TV Series
Eighties hit Beverly Hills Cop is set for TV return
Jurassic Park to Re-open its Gates
A release date has finally been announced for the long awaited Jurassic Park 4
Oscar Nominations Announced – Lincoln Leads the Way
Oscar nominations for 2013 have been announced, with Steven Spielberg’s Lincoln leading the way
US Release for Carrie Remake Pushed to October
US Carrie fans will have to wait a further six months before seeing the new version, as the film’s release gets pushed back to October 2013.
Jack Reynor Joins Cast of Transformers 4
Irish actor Jack Reynor has been cast by Michael Bay to star opposite Mark Wahlberg in Transformers 4
Rise and Shine for Sundance 2013
Our preview of this year’s upcoming Sundance Film Festival
Julia Roberts Lands Starring Role in HBO Adaptation
Erin Brokovich actress a definite for next year's film version of The Normal Heart
2013 BAFTA Nominations Announced
The 2013 BAFTA nominations have been announced! Spielberg's Lincoln and Hooper's Les Misérables receive the most nods. Read the full nominations here.
Joseph Gordon-Levitt to be Cast in Marvel’s Guardians
Joseph Gordon-Levitt is rumoured to be up for a part in Marvel’s new project, Guardians of the Galaxy
Trekkie Receives Ultimate Christmas Gift
How a terminally ill Star Trek fan's final wish came true..
Michael Haneke Withdraws Amour from Austrian Film Awards
Director Michael Haneke has withdrawn Amour from the Austrian Film Awards after it became clear the film isn’t eligible for some of the top prizes
New Year Honours: Ewan McGregor to Receive OBE
Actor Ewan ‘Obi-Wan’ McGregor is to be knighted in the New Year Honours List
Creator of Thunderbirds Gerry Anderson Dies at 83
Gerry Anderson, creator of Thunderbirds, has died at the age of 83.
Director Spike Lee says he won’t see Django Unchained
Director Spike Lee has said that he won’t be seeing Quentin Tarantino’s Django Unchained as it is disrespectful to his ancestors
Daniel Day-Lewis to Receive Montecito Award
Daniel Day-Lewis will be presented with a prestigious award at the 2013 Santa Barbara International Film Festival

domain: entertainment


NCC Group advises on UK Government’s Manual for Smart Streets
NCC Group is proud to have collaborated with the Department for Transport (DfT) and the Transport Technology Forum (TTF) on the development of the Manual for Smart Streets (MfSS), a newly launched guide for local authorities and other key stakeholders in road infrastructure.
Darren Capes, ITS Policy Lead and TTF Manager, Traffic and Technology Division, Department for Transport commented: “NCC Group's Transport Practice have been instrumental in supporting the Department for Transport with the development of the Manual for Smart Streets that has now been published through the Transport Technology Forum, by providing invaluable cyber security guidance that is embedded into the Manual’s core use cases for digital service development.”
Published online, the MfSS supports authorities who are conceiving, designing and implementing smart street systems. It has been created to provide guidance, examples and practical pointers, allowing authorities to make best use of technology and improve how we travel.
Local authorities are working towards the challenging goals of achieving net zero, making transport more accessible and revitalizing regional economies. This has led authorities to embrace new connected and automated road technologies, which come with new risks such as cyber security.
The MfSS is intended as working content that continues to evolve with emerging practice and technology. It aims to encourage engagement with the TTF and wider practitioner community, such as NCC Group, to further develop the content of this manual and best practices for smart streets.
Alexander Patton, a transport security specialist with NCC Group, led a six-month engagement with local authorities, suppliers and industry bodies across the UK before developing the MfSS Cyber Security Guidance. This includes an analysis of the current state of smart streets security, relevant case studies and guidance on managing security risk through the smart streets lifecycle.
Funded by the DfT and InnovateUK, the Transport Technology Forum exists to help meet the need for action to deliver the future of mobility by driving more effective and efficient management of existing and new road networks.
Liz James, Senior Security Consultant at NCC Group said: “The Manual for Smart Streets is a pivotal step for the smart transport sector. By providing guidance and examples on how to operate smart infrastructure, it empowers local authorities and stakeholders to make informed decisions and consider security from the design phase.
“While not regulation, its impact is set to be far-reaching, as it emphasizes the need to prioritise security throughout the entire process. We’re proud to have contributed to this landmark piece of work that distils expert knowledge into a layman's terms readable document, that can continually evolve and has application in the real world.”
You can view the Manual for Smart Streets online at: https://ttf.uk.net/manual-for-smart-streets/


Fake branded make-up containing traces of lead were among a haul of more than 1,000 counterfeit goods seized by Isle of Wight Council Trading Standards officers in an undercover sting.
Rip-offs of major clothing labels such as Nike and SuperDry, as well as knockoff cosmetics advertised as Chanel, Kylie and Mac, were recovered from a property in East Cowes, following a raid in February 2018.
Eight-month prison sentence
Last week, Steven Mather and Michelle Aldridge appeared at the Isle of Wight Crown Court for sentence after admitting their role in the counterfeit operation which the court heard originated in Manchester.
Mather, 34, was handed an eight-month prison sentence, suspended for 24 months, after admitting two counts of unauthorised use of a trademark and a further offence of distributing cosmetics containing a prohibited substance.
Community order for aiding and abetting
Aldridge, 47, was given a 12-month community order after pleading guilty to two counts of aiding and abetting Mather.
The court heard Trading Standards launched an investigation after receiving complaints fake designer fashion was being sold locally through Facebook.
Over 1,000 fake goods discovered
The probe led investigators to an address in Beatrice Avenue where a search of the property uncovered more than 1,000 fake goods in an upstairs bedroom and in the loft.
The items included counterfeit clothing and shoes of well-known brands such as Ted Baker, Adidas and Nike, as well as bogus cosmetics and other branded products including Yankee Candles.
Traces of lead in makeup
The court was told samples of the makeup were sent for analysis and some were found to contain traces of lead.
Mather told investigators he obtained the goods from a man in the Manchester area and sold them under a Facebook profile he set up in the name of ‘Michelle Aldridge’. He said he did this in order to appear more trustworthy to female customers.
Mather: “Taken advantage of by others”
In mitigation, Mather, who pleaded guilty at the first opportunity, said he made very little profit from the operation and felt he had been taken advantage of by others.
As well as a suspended jail term, Recorder Anna Midgley ordered him to carry out 100 hours of unpaid community work and pay prosecution costs of £400.
Aldridge was given 12 Rehabilitation Activity Requirement Days as part of her community order, but there was no order for costs.
Peace: “If it sounds to be good to be true, it more than likely is”
Speaking after the case, Councillor Gary Peace, the council’s Cabinet member for community safety, said:
“Counterfeit goods need to be taken off the streets as these products do not go through the correct safety checks that legitimate products do.
“In this case, some of the make-up samples contained lead which is particularly concerning as we believe these cosmetics would have been appealing to teenagers.
“Counterfeit clothes and other products are usually very poor quality and don’t undergo any safety tests at all. If it sounds to be good to be true, it more than likely is, and we will continue to investigate any intelligence that we are given to take these products off the market.”
Trading Standards welcome any information from the public on the sale of counterfeit products from any premises or people.
To report anyone selling counterfeit products on the Island, call (01983) 823000.


EconPapers - Sustainability - Volume 14.
Spatial planning faces challenges in addressing interactions between land and sea. This paper elaborates on land–sea interfaces, which can integrate certain socio-cultural values and related tensions into maritime spatial planning (MSP). In this article, three regional case studies from Estonia, Latvia, and Poland analysed important intersections between the formations of cultural values and spatial dynamics within MSP processes. These cases make it possible to address current challenges, contested boundaries, and spatial planning possibilities to embrace the vibrant and complex ways the sea becomes connected to societal change. The study indicates the multiplicity of land–sea interfaces, which should be involved in MSP through situated places of terraqueous interactions, means of public participation, and meaningful boundaries within mobilised co-existence. The actual and possible tensions in allocating new functions of maritime spaces indicate the importance of coastal landscapes and communities. Thus, MSP practice can employ the land–sea interfaces to advance regional planning through participatory engagements, which reveal sociocultural linkages between society and environment on coastal areas.
Questions this practice may help answer:
- How to integrate socio-cultural values and related tensions into MSP?
- How to address Land-Sea Interaction (LSIs) within MSP processes?
- How can participatory engagement strengthen the implementation potential of MSP?
This research is conducted within the framework of the Interreg Land–Sea-Act project.
Aspects / Objectives:
This paper elaborates on land–sea interfaces, which can integrate certain socio-cultural values and related tensions into maritime spatial planning (MSP). Three regional case studies from Estonia, Latvia, and Poland are analysed to describe the significant intersections between the formation of cultural values and spatial dynamics within MSP processes. These cases aim to address current challenges, contested boundaries, and spatial planning possibilities to embrace the vibrant and complex ways in which the sea becomes connected to societal change.
These cases analysed the sociocultural dimension of land–sea interactions in MSP by specifically focusing on certain interfaces, thereby highlighting and framing co-existences between land and sea. These three case studies build on materials from the Interreg project Land–Sea-Act, as well as participatory processes (focus group, interviews, workshops, participatory GIS, scenarios) and desktop studies (statistical information, spatial data, policy, and planning documents).
Main Outputs / Results:
The paper contributes to understanding the sociocultural dimensions of maritime space by focusing on the multiplicity of land–sea interfaces in MSP. The three case studies in the Baltic Sea region indicate that land–sea interfaces in MSP are related to situated places of terraqueous interactions, means of public participation, and meaningful boundaries within mobilised co-existence with the sea. This perspective provides some tools for further elaborating the urgent need to address land–sea interactions in long-term MSP.
The cases reveal the importance of carefully addressing the sociocultural aspects of land–sea interfaces in MSP and call for identifying ways to bridge how diverse stakeholders form meanings and planners rationalise ideas of culture and of place across marine and terrestrial boundaries. In this sense, the elements explored in this article can feed into MSP processes across Europe.
Centre for Landscape and Culture, School of Humanities, Tallinn University, 10120 Tallinn, Estonia
Costs / Funding Source:
INTERREG BSR: #R098 Land–Sea-Act; Polish Minister of Science and Higher Education:
5063/INTERREG BSR/19/2020/2; Estonian Research Council: PRG398.
Tarmo Pikner - Tallinn University - tarmo.piknertlu.ee


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Whitsundays Day Tour
ZigZag Whitsundays is a 1 Day tour of the Whitsunday Islands including 2 Beaches, Whitehaven Beach and another, 2 snorkel locations and a resort.The Whitsunday Islands are one of the last truly unspoiled regions on the East Coast where natural attractions are a prelude to complete relaxation. Pristine and beautiful in every way, this region comprises of 74 islands in total and stretches along the coast from Mackay to Bowen. Although extremely remote, you can reach this paradise on ZigZag‚Äôs Whitsundays Day Trip and explore everything this coastline has to offer.
ZigZag is a 39ft Naiad with 4 engines totaling 1200 horsepower. This gives us a cruising speed of 24knotts and top speed of 48knotts. This makes the vessel a fast, yet extremely comfortable ride allowing guests of all ages and adventure levels to participate. Our full day tour of the Whitsundays takes 36 guests and includes morning tea, lunch and afternoon snacks. You will be supplied with stinger suits and snorkeling equipment onboard the boat.
Morning Tea, Lunch, Afternoon Tea, Wetsuit, Mask, Snorkel, Fins, Tap Water
What to Bring:
Sunscreen, hat, towel, cash or credit card (for onboard purchases and drinks at Palm Bay Long Island Bar)

domain: travel


text: Here a romantic palace, there a fortified castle, and somewhere in between an old stable. Living history around every corner. And the real draw here, not only the grand castles and palaces of Meissen, Dresden, Torgau and Nossen but also the inimitable blending of centuries-old architecture and landscape design. Set amid wide and visually pleasing forest and lakeland landscapes, for example, is the picturesque summer palace of Moritzburg. Who wouldn’t be enticed by its unmatched tranquillity and character? Just as if you’d stepped back in time.


On December 15, 2023, the Supreme Court of Maryland ordered the reinstatement of attorney Sheron A. Barton to the practice of law in the state. Barton had been suspended indefinitely in March 2015 by the Court of Appeals for failing to competently represent nine bankruptcy clients, neglecting their legal matters, and aiding the unauthorized practice of law.
The case is entitled “In the Matter of the Petition for Reinstatement of Sheron Andrea Barton,” with case no. AG No. 13.
According to The Daily Record, Barton was the owner and sole attorney at the Cardinal Law Firm in Camp Springs from 2010 to 2011. An investigation by the Attorney Grievance Commission found that Barton failed to competently represent nine bankruptcy clients and neglected their legal matters.
The Commission discovered Barton allowed an unlicensed employee, Richard Tolbert, to provide legal advice to clients, constituting aiding the unauthorized practice of law. Barton also violated the rules by failing to keep unearned fees in an attorney trust account and instead depositing them into the firm’s operating account.
During a January 2014 evidentiary hearing, Barton testified that Tolbert’s responsibilities were limited, but the hearing judge did not find her testimony credible. Four former clients testified that Barton showed up unprepared at hearings, failed to keep them informed about their cases, accepted payment but did no work, and did not refund unearned fees despite court orders.
In ordering the indefinite suspension in 2015, the Court of Appeals found Barton embodied a dishonest and selfish motive, engaged in a pattern of misconduct, committed multiple offenses, and refused to acknowledge the wrongful nature of her conduct.
In its order, the Supreme Court said Barton had satisfied the conditions of her reinstatement by demonstrating remorse and rehabilitation from the misconduct that led to her suspension over eight years ago. She will once again be permitted to practice law in Maryland.
According to LinkedIn, Ms. Barton works as a lead Compliance Officer at Wells Fargo. She graduated from Nova Southeastern University Shepard Broad College of Law, and she has been licensed to practice in Maryland.
A copy of the original filing can be found here.


Losliya one of the most prominent stars of ‘Bigg Boss 3’ lost her father Mariyanesan reportedly due to a massive heart attack. Mariyanesan was in Canada for the past several years to make a living and support his family back in Sri Lanka. It is said that due to the coronavirus pandemic complications have raised with regards to transporting his body to his home country. Appeals have been made to the foreign ministers of both Canada and Sri Lanka to help the family with the formalities.
Vanitha Vijayakumar who was also one of the contestants in ‘Bigg Boss 3’ has updated that she spoke to Losliya who is in deep grief and is crying inconsolably. She has also revealed that Vijay TV management is aiding Losliya in speaking to the embassy to help her fly to Sri Lanka from Chennai.
Losliya’s mother and two sisters live in Sri Lanka and the actress is in Tamil Nadu due to her acting commitments in films such as ‘Friendship’ and two other untitled ones.
To all fans …I spoke to her she is devastated and crying..but she will be strong she's trying to fly to srilanka.arranging thru embassy team is with her.due to covid pandemic the body can't reach srilanka immediately.ive given her my love and blessings https://t.co/nWMvNV9Xms


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Monday, January 16, 2012
Luis Aparicio Collection - 2011 Topps Lineage 1975 Mini
We finally grabbed the 2011 Topps Lineage Luis Aparicio 1975 mini card. When Lineage first came out there were several of these on eBay but all went for more than we wanted to pay. Our patience paid off and we found one for a good price. It's nice to be able to cross this one off our want list.

domain: hobby


Champaign, IL, Sporting Alert — No. 6 Wisconsin was stunned 24-23 by Illinois in the shock of Week 8 so far on Saturday, Oct. 19.
James McCourt converted a 39-yard field goal as time expired to seal the win for the Fighting Illini over the previously undefeated Badgers, who came into this game as a 30.5-point favorite, according to Caesars Sportsbook.
It was the largest upset thus far this campaign.
The win by Illinois at home this weekend was its first over a Top 10 team since 2007.
Wisconsin, meanwhile, led 13-7 at halftime, but failed to pull away and it cost them in the end, as the homes side came back to outscore the travelers 17-10 in the second half.
Jack Coan passed for 264 yards with one touchdown and an interception for Wisconsin (6-1, 3-1 Big Ten), which must quickly regroup ahead of the trip to No. 4 Ohio State, which easily put away Northwestern 52-3 on Friday night.
Jonathan Taylor ran for 132 yards and a score and Jake Ferguson ran for a score and 77 yards in the defeat for the Badgers.
Illinois’ (3-4, 1-3) Brandon Peters complete just 9 of 21 passes for 174 yards, but threw two touchdown passes, including one late in the fourth quarter to help trim the deficit to 23-21 before the field goal.
This was the second-biggest upset in a Big Ten game over the last 40 seasons, according to ESPN Stats and Information. (Northwestern defeated Minnesota in 1982 as 32-point underdogs in that game)


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Introduction of ‘The Mongoose and the Quarrelsome Neighbours’ Panchatantra Story | Summary
“The Mongoose and the Quarrelsome Neighbours” is a story from the ancient Indian collection of fables and folk tales called the Panchatantra. The story teaches the importance of being a good neighbor and the dangers of being quarrelsome.
In the story, there is a mongoose who lives in a village with several other animals, including a pair of quarreling neighbors, a jackal and a crow. The mongoose tries to mediate their disputes and encourage them to live in harmony, but the jackal and crow are stubborn and continue to argue.
One day, a venomous snake appears in the village and the mongoose bravely fights and kills it, saving the lives of the jackal and crow. The two neighbors are grateful to the mongoose and finally see the value of living in peace with their neighbor.
The story serves as a cautionary tale about the importance of being a good neighbor and the dangers of constantly quarreling with those around us. It also demonstrates the bravery and selflessness of the mongoose, who was willing to risk its own life to protect its neighbors.
The Mongoose and the Quarrelsome Neighbours – Panchatantra Story
Once upon a time, in a small village, there lived a mongoose who was known for his wit and intelligence. He lived near a group of quarrelsome neighbours who were always fighting and bickering with each other.
One day, the mongoose decided to teach the neighbours a lesson in harmony. He went to each of their houses and pretended to be gravely ill. When the neighbours asked him what was wrong, the mongoose told them that he had been bitten by a venomous snake and needed their help.
The neighbours, who were touched by the mongoose’s plight, put aside their differences and worked together to find a cure for the mongoose. They searched high and low for the right herbs and roots, and eventually, they were able to prepare a remedy that would cure the mongoose’s venomous bite.
The mongoose, who had faked his illness, was overjoyed to see the neighbours working together. He thanked them for their kindness and told them that he had only pretended to be sick to teach them a lesson in harmony.
From that day on, the quarrelsome neighbours learned to live in peace and harmony, and the mongoose became a beloved member of the community.
Moral of the Story
The moral of the story is that cooperation and teamwork can help to resolve conflicts and bring people together. The mongoose’s clever trick showed the neighbours that by working together and putting aside their differences, they could achieve a common goal and live in harmony. The story also highlights the importance of empathy and compassion, as the neighbours were moved by the mongoose’s apparent suffering and were willing to help him.


We bring to you our large auto parts register order forms that will take away all the hassles of maintaining the inventory in keeping with the sales register.
Printed on NCR paper, these forms have especially been designed for auto sales and cover all the basics like the part sold, its numbers, price and even the tax levied. The inventory tab on the form is the best way to not only record the sale but also maintain an inventory at the same time.
With two tasks getting done at one go, it is no wonder that these forms are selling like hot cakes for auto part stores.
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Trauma Surgery, General SurgeryGeneral Surgery
A surgeon manages a broad spectrum of surgical conditions affecting almost any area of the body. The surgeon established the diagnosis and provides the preoperative, operative, and postoperative care to surgical patients and is usually responsible for the comprehensive management of the trauma victim and the critically ill surgical patient. The surgeon uses a variety of diagnostic techniques, including endoscopy, for observing internal structures, and may use specialized instruments during operative procedures. A general surgeon is expected to be familiar with the salient features of other surgical specialties in order to recognize problems in those areas and to know when to refer a patient to another specialist. and Surgical Critical CareSurgical Critical Care
A surgeon with expertise in the management of the critically ill and postoperative patient, particularly the trauma victim, who specializes in critical care medicine diagnoses, treats and supports patients with multiple organ dysfunction. This specialist may have administrative responsibilities for intensive care units and may also facilitate and coordinate patient care among the primary physician, the critical care staff, and other specialists..
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domain: medical


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Indigenous All Stars player Josh Kerr has called on the Australian Rugby League Commission (ARLC) to send an Indigenous side in place of the Kangaroos to the World Cup later this year.
- Indigenous All Stars player Josh Kerr calls on the ARLC to send a First Nations side to the rugby league World Cup
- Kerr says there is strong support among Indigenous players to go to the UK for the tournament
- Last week, Australia and New Zealand pulled out of the event, citing player welfare and safety concerns.
Last week, the ARLC and New Zealand Rugby League announced they were pulling out of the tournament "because of player welfare and safety concerns" surrounding the COVID-19 situation in the United Kingdom.
Kerr said it could be the perfect opportunity for the Indigenous All Stars.
"Lo and behold, Australia and New Zealand pull out of the World Cup, and straight away on our group chat it was, 'how good would that idea be if we can put it forward?'.
"Next thing, it sort of caught fire and every man and his dog wanted to jump onboard."
Kerr also said the opportunity to represent his culture outweighed any concerns surrounding the pandemic.
"A lot of people wouldn't understand that, they are worried about COVID … but straight away all the boys were super keen because they know what it means for our community and our people," he said.
"But fingers crossed, it's crazy times and I hope we can get it in.
"When I got to represent the Indigenous All Stars it was a dream come true for me and my family … I am shaking and sweating just thinking about it, let's get behind it, let's sign a petition to get it going."
'Too great a risk'
ARLC chairman Peter V'Landys said last week the pandemic posed too great a risk to players and officials.
"In the current environment, the risks to the safety, health and wellbeing of the players and officials travelling from Australia to participate in the tournament this year are insurmountable," Mr V'landys said.
He also said there were concerns about player welfare after living in the rugby bubble for an extended period.
"The majority of NRL players are currently living away from home under difficult biosecurity protocols," he said.
"They would then be required to remain under protocols and away from home for the duration of the tournament before again quarantining on return to Australia.
The NRL season is set to wrap up in early October, and England is supposed to be hosting 16 teams for a World Cup running from October 23 to November 27.


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Because of the assorted value of R you may use to fix an issue. If it’s not in Kelvin you are going to be wrong. Only through appropriate value of R are you going to get the right answer of the issue. K and this is only so it is possible to cross it out. The combined gas law demonstrates how the 3 variables are related to one another.
Ok, I Think I Understand Chemistry Gas Laws Worksheet, Now Tell Me About Chemistry Gas Laws Worksheet!
Chemistry can be a rather challenging class for a few of our students. Students will also think of the interdependence of several diverse kinds of life within one ecosystem. Certainly in the united kingdom exam system, it is very rare for chemistry students to be expected to know both of these laws today.
So our pressure in this instance, we’re looking for. paper writer There are lots of means. So this mix of things is the perfect gas law.
Freezing is the procedure of changing liquid matter into solid issue. Another method of stating this law is to say that heat can’t flow, alone, from a region of cold to a region of hot.
Introducing Chemistry Gas Laws Worksheet
It can help you differentiate when and where to utilize it. I’m certain that this will help you too. Ensure you’re conscious of that.
The answers to the questions can be found separate worksheets so that you can fill them out and check your work. It’s very likely additionally to locate the Phonics Worksheet. The worksheet contains explicit instructions.
Apparently, the barrier is going to be forced to the right, and the amount of the gas increases. So that the thermometer is only to monitor the temperature change and the entire assembly with the valve on the bottle is merely to make it simple to alter the pressure rapidly. Predict what it would be if the pressure were 8.00.
The Number One Question You Must Ask for Chemistry Gas Laws Worksheet
You may upload your own personal and boost your image. online paper writer The calendar lets you know what’s going on in class daily. Despite a template that’s superior you may not have a deal on the place to start. This range of mols, it’s the very first time it’s been introduced.
Make sure all the volumes, temperatures, and moles are in their various units before plugging it in the formula. Assume that the temperature and amount of the gas stay constant. Different types of gas doesn’t have an effect on ratio of expansion or compressibility. It will increase by T, and the pressure will increase.
So now I would like to acquire grams by itself so I’m likely to multiply either side by molar mass. Therefore, there are a profusion of possible ground states for the perfect gas, meaning its entropy is nonzero at zero temperature. A single atom, on the flip side, has a mass that’s just a little fraction of a gram. In both situations, increasing pressure results whenever the amount of the gas is reduced. We can use the perfect gas equation to figure out the volume of 1 mole of a perfect gas at 0C and one atmosphere pressure. Use the perfect gas equation.
Electrons take part in bonding, and for that reason, their arrangement in an atom is extremely important. Chart summarizes chemical and physical qualities of the 3 planets. The mixture of transmission lines and substations is vital to powering up your homes and companies. And also ideally in a perfect world, we’re saying that gas particles don’t have any intermolcular forces meaning they don’t attract or repel from one another and we know that’s not correct.
Each High School Physics problem comprises an explanation of the appropriate answer, so once you skip an issue, you can learn where you went wrong. Energy is the capacity to bring about change or to do work. Second assistance of the report focuses on the risks and risks of mining and using coal. These will genuinely help your comprehension of Charles’s Law if you take some time to do the digital experiments! Also referred to as Conservation of Matter.
Play around with it and figure out how it acts differently from a usual liquid and a typical solid. Gas particles do have some type of volume. Be sure to create the joint is air tight.
For gases it’s a little more complicated. Therefore, an alternate kind of the perfect gas law might be useful. If you know the best gas law, you don’t need to understand any other gas laws, for it’s a combo of the rest of the laws.
The Do’s and Don’ts of Chemistry Gas Laws Worksheet
I don’t know precisely what constitutes required expenses below this law. Potential energy, as its name implies, is energy that hasn’t yet been used, thus the expression potential. Some folks call it pivnert to keep in mind the perfect gas law.
For an extremely rough idea of the whole dollar sum, multiply each ratio by twelve and then by the amount of years you’ll be paying the mortgage. As soon as you get a rough notion, speak to a mortgage lender at a better estimate of how much you will have the ability to borrow. You have to consult a mortgage lender afterwards to find a more precise estimate.
Ideas, Formulas and Shortcuts for Chemistry Gas Laws Worksheet
Questions on a particular page tend to construct upon previous questions so that a conceptual comprehension of the topic is gradually developed. This tool is intended to help users determine what sort of environmental permits they need and offer the forms to apply for these permits. The Final Result is your reaction to the matter of the larger number the smaller number. Click the link to learn more.

domain: chemistry