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The yeast strains IPM32-16, ISM28-8sT, and IPM46-17, isolated from plant and soil samples from Iriomote Island, Japan, were explored in terms of lipid production during growth in a mixture of glucose and xylose. Phylogenetically, the strains were most closely related to Cystobasidium slooffiae, based on the sequences of the ITS regions and the D1/D2 domain of the LSU rRNA gene. The strains were oleaginous, accumulating lipids to levels > 20% dry cell weight. Moreover, kinetic analysis of the sugar-to-lipid conversion of a 1:1 glucose/xylose mixture showed that the strains consumed the two sugars simultaneously. IPM46-17 attained the highest lipid content (33%), mostly C16 and C18 fatty acids. Thus, the yeasts efficiently converted lignocellulosic sugars to lipids, aiding in biofuel production (which benefits the environment, promotes rural jobs, and strengthens fuel security). The strains constituted a novel species of Cystobasidium, for which we propose the name Cystobasidium iriomotense (type strain ISM28-8sT = JCM 24594T = CBS 15015T).
Citation: Tanimura A, Sugita T, Endoh R, Ohkuma M, Kishino S, Ogawa J, et al. (2018) Lipid production via simultaneous conversion of glucose and xylose by a novel yeast, Cystobasidium iriomotense. PLoS ONE 13(9): e0202164. https://doi.org/10.1371/journal.pone.0202164
Editor: Marie-Joelle Virolle, Universite Paris-Sud, FRANCE
Received: March 8, 2017; Accepted: July 30, 2018; Published: September 12, 2018
Copyright: © 2018 Tanimura et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All sequencing files are available from the GenBank database (accession numbers: AB726384, AB726571, AB726474).
Funding: This work was supported in part by the Advanced Low Carbon Technology Research and Development Program (ALCA, grant no. JPMJAL1607) of the Japan Science and Technology Agency (JST). MT was partially supported by a KAKENHI (no. 26650148) from the Japan Society for the Promotion of Science (JSPS). This work was also supported in part by the RIKEN Competitive Program for Creative Science and Technology and by a grant from the Biomass Engineering Program of the RIKEN Center for Sustainable Resource Science (to MO).
Competing interests: The authors have declared that no competing interests exist.
Recently, chemical and fuel production from lignocellulosic biomass is receiving increasing attention [1, 2]. Hydrolysates of such biomasses contain mixtures of sugars, mainly glucose and xylose in various ratios [3, 4]. Complete conversion of sugars in hydrolysates is necessary for efficient utilization of lignocellulosic biomass. The production of microbial lipids via enzymatic degradation of lignocellulosic biomass is currently a subject of intense interest. However, one major barrier to commercial application is the cost of the enzymes used to degrade cellulose to monosaccharides. The absence of a high-performance microorganism (a strain that can efficiently convert released sugars to lipid) renders practical production difficult.
Some yeasts accumulate lipids to over 20% of the cell dry weight and are thus termed oleaginous yeasts . The carbon-chain lengths of the accumulated fatty acids typically range from 12 to 24, with the major components being palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), and linoleic acid (C18:2) [6, 7]. The composition is similar to that of plant oils ; therefore, these lipids can be used as feedstocks for biofuels and oleochemical products. Lignocellulosic biorefineries can achieve greater reductions in CO2 emission than petroleum-based biorefineries. However, such refineries are complex and expensive to build . It is anticipated that recent progress in lignocellulosic biorefinery technology will decrease production costs. High-value-added lipids, such as middle-chain fatty acids for use in health foods, are synthesized by oleaginous yeasts. Currently, such lipids are produced from animals and plants; they are expensive and not economically competitive. If lignocellulosic biomass could be used as feedstock, the cost of such lipids could be reduced, and new industries could develop.
Several groups have attempted to produce lipids by culturing oleaginous yeasts on hydrolysates derived from lignocellulosic biomass [10–13]. However, yeasts use glucose in preference to xylose, the uptake of which commences only after glucose is depleted, a phenomenon termed glucose repression [14, 15]. Such sequential utilization prolongs the conversion period and renders the process uneconomic. Efforts have been made to encourage sugar co-conversion, such that glucose and xylose are simultaneously converted to lipids. Zhao et al.  optimized the concentrations of sugars, nitrogen sources, and minerals, and achieved a lipid content of 61% dry weight using a medium containing 48.9 g/L glucose and 24.4 g/L xylose. It was concluded that lipid-accumulating ability was influenced by the concentrations of sugars, yeast extract, and FeSO4. Unfortunately, the sugar consumption pattern remained unclear, and rigorous preparation of the recommended medium may not be practical. Anschau et al.  compared batch, fed-batch, and continuous cultures. Continuous cultivation in a medium with 20 g/L glucose and 45 g/L xylose yielded a high lipid content of 49% dry weight. Both sugar were consumed simultaneously, but half remained in the broth. Glucose repression remains a significant barrier to efficient sugar conversion; optimization of conversion has not yet been achieved. Identification of oleaginous yeasts capable of simultaneous glucose and xylose conversion is thus critical when seeking to improve lipid production efficiency. Efforts have been made to find such yeast. For example, Cutaneotrichosporon cutaneum (formerly Trichosporon cutaneum) [3, 17] and Geotrichum fermentans (formerly Trichosporon fermentans)  engage in simultaneous glucose and xylose consumption from detoxified lignocellulosic hydrolysates. In addition, it is well-known that the oleaginous yeast Lipomyces starkeyi uses glucose and xylose simultaneously to produce lipids [16, 18].
When surveying Japanese isolates, we discovered yeasts exhibiting high-level oleaginous potential under various conditions [17, 19, 20]. In the present study, we focused on oleaginous yeasts that could utilize glucose and xylose simultaneously and selected three strains, IPM32-16, ISM28-8sT, and IPM46-17, for evaluation. These were phylogenetically close to Cystobasidium slooffiae, C. fimetarium, and C. minutum. To assess sugar assimilation patterns, we performed kinetic analyses of the lipid production using a mixture of glucose and xylose. In addition, based on both sequence analyses and phenotypic characterization, we concluded that our strains belonged to a novel species within the genus Cystobasidium, for which we propose the name Cystobasidium iriomotense f.a. sp. nov. (type strain ISM28-8sT = JCM 24594T = CBS 15015T).
Materials and methods
Strains and media
The new strains were isolated from plant and soil samples collected on Iriomote Island in the Iriomote Ishigaki National Park, Japan  (Table 1). A reference strain, C. slooffiae JCM 10954T, was obtained from the Japan Collection of Microorganisms (JCM) at the RIKEN BioResource Center (http://jcm.brc.riken.jp/en/). C. slooffiae was an appropriate control, because the strain was (1) phylogenetically most closely related to the isolates; (2) capable of glucose and xylose assimilation ; and (3) capable of accumulating lipids . YM agar medium (Difco, Detroit, MI, USA) was used for yeast pre-culture and maintenance. The 1:1 (w/w) glucose/xylose (GX) medium was based on the medium of Gong et al. , and contained ammonium sulfate 1 g/L, yeast extract 0.5 g/L, potassium dihydrogen phosphate 1 g/L, magnesium sulfate heptahydrate 1 g/L, glucose 10 g/L, and xylose 10 g/L. We used a 1:1 glucose-to-xylose weight ratio to simplify our analyses.
Sequencing and phylogenetic analysis
DNA fragments, including the internal transcribed spacer (ITS) regions plus the D1/D2 domain of the LSU rRNA gene, were amplified directly from yeast cells. Cells were suspended in 60-μL amounts of Prepman Ultra Sample Preparation Reagent (Applied Biosystems, Foster City, CA, USA) and template DNA prepared according to the manufacturer’s instructions. The ITS regions, including the 5.8S rRNA gene and the D1/D2 domain of the LSU rRNA gene, were amplified using the primers 5’-AACTTGGTCATTTAGAGGAA-3’  and NL4 . The PCR products were directly sequenced using an ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit (Applied Biosystems) and analyzed with an Applied Biosystems sequencer model 3100, according to the manufacturer’s instructions. The sequences including the ITS regions and D1/D2 domain of the LSU rRNA gene are available from the DDBJ/GenBank/EMBL database, as shown in Table 1. Reference sequences used in the phylogenetic study were obtained from the DDBJ/GenBank/EMBL database . Multiple alignment was performed using MEGA7 software . A phylogenetic tree was constructed using the maximum likelihood method of MEGA7. The Tamura-Nei model  was used for analyses. Initial trees for the heuristic search were automatically obtained by applying the neighbor-joining and BIONJ algorithms to a matrix of pairwise distances estimated using the maximum composite likelihood (MCL) approach and then selecting the topology with the highest log likelihood value. Bootstrap analysis was performed 500 times .
Most of the morphological, physiological, and biochemical characteristics were examined as suggested by Kurtzman et al.  Assimilation of nitrogen compounds was investigated on solid media using starved inocula. Sexual reproduction tests were performed on YM and cornmeal agar (individual or paired strains) at room temperature.
Single loops of 3-day-old yeast colonies were suspended in 100 mL amounts of GX medium in Erlenmeyer flasks and incubated at 28°C, with rotary shaking at 150 rpm, for 10 d. Broth was withdrawn at various times. The levels of intracellular lipids and sugars were determined. All experiments were performed in triplicate.
Measurement of fatty acids
Total intracellular lipid contents were estimated as total fatty acids. Accumulated lipids were extracted from lyophilized cells using a hydrochloric acid-catalyzed direct methylation method . In brief, after cultivation, the centrifuged cells were lyophilized and weighed, dissolved in toluene and methanol, and directly transmethylated with 8% (v/v) methanolic HCl at 100°C for 1 h. The resultant fatty acid methyl esters were extracted with n-hexane and analyzed using a gas chromatograph (GC-2010 Plus; Shimadzu, Kyoto, Japan) equipped with a flame ionization detector (FID) and an autosampler (AOC20; Shimadzu). A TC-17 capillary column (GL Science, Tokyo, Japan) was used. The elution temperature commenced at 165°C for 2 min and then increased by 5°C/min to 180°C, followed by a hold for 5 min, an increase at 5°C/min to 240°C, and an additional hold for 3 min. Helium at 2.0 mL/min served as the carrier gas, and nitrogen as the make-up gas. The injector temperature was 250°C and the detector temperature was 260°C, with a split ratio of 50:1. Major peaks were identified by their retention times using standards obtained from Sigma-Aldrich (St. Louis, MO, USA). Heptadecanoic acid (C17:0) served as an internal standard for the determination of fatty acid concentrations.
Residual glucose and xylose concentrations were determined using a high-performance liquid chromatograph (Shimadzu) equipped with an Aminex Fermentation Monitoring Column (Bio-Rad Laboratories, Hercules, CA, USA) and Micro-Guard Cation H Refill Cartridges in a Standard Cartridge Holder (Bio-Rad Laboratories). The detector was an RID 10A refractive index detector (Shimadzu). The column was held at 60°C using a CTO 20A column oven (Shimadzu). A sulfuric acid solution (5 mM) served as the mobile phase at a constant flow rate of 0.6 mL/min.
Phylogeny and phenotypic characteristics
A phylogenetic tree based on the sequences of the ITS regions plus D1/D2 domain of the LSU rRNA genes showed that the three strains clustered with Cystobasidium slooffiae, C. fimetarium, and C. minutum (Fig 1). The sequence differences in the ITS region ranged from 2 bp (including one gap) to 5 bp (including one gap), and those in the D1/D2 domain ranged from 0 to 2 bp; indicating that the three strains belonged to the same species [32–34]. Of phylogenetically closely related species, the differences between our species and Cystobasidium fimetarium, and C. slooffiae and C. minutum, were 8–10 bp and 9–11 bp respectively (Fig 1), suggesting that our strains constituted a novel species [33, 35]. In addition, our species was phenotypically distinct from the phylogenetically closely related species C. fimetarium , C. slooffiae [22, 37] and C. minutum [22, 37] with respect to several traits: namely, galactose assimilation and the inability to use D-ribose, DL-lactate, or xylitol as the sole carbon source (Table 2). Thus, we propose the name Cystobasidium iriomotense. Our strains utilize not only xylose, but also cellobiose or L-arabinose as the sole carbon source (Table 2). We anticipate that they will be industrially useful, but we have not yet determined the lipid accumulation abilities using these sugars.
The evolutionary history was inferred using the maximum likelihood method based on the Tamura-Nei model . The tree with the highest log likelihood (-3830.1264) is shown. The rate variation model allowed for some sites to be evolutionarily invariable ([+I], 38.4432% sites). The tree is drawn to scale; the branch lengths indicate the number of substitutions per site. All positions containing gaps and missing data were eliminated. A total of 1,047 positions were present in the final dataset. Bootstrap values < 50% are not shown.
Lipid accumulation during growth on GX medium
To explore the time course of conversion of sugars to lipids by IPM32-16, ISM28-8sT, IPM46-17, and C. slooffiae JCM 10954T, GX medium containing 10 g/L glucose and 10 g/L xylose as sole carbon sources was used. All strains utilized glucose and xylose simultaneously rather than sequentially (Fig 2). After cultivation, 99.5, 85.3, and 99.2% of the initial sugars were consumed by IPM32-16, ISM28-8sT, and IPM46-17, respectively. In contrast, the figure for C. slooffiae JCM 10954 was 31.9%. In addition, as shown in Fig 2, the curves were biphasic: the log phase persisted from d 0 to 4, followed by the stationary phase from d 4 to 10, except for C. slooffiae JCM 10954T. The strains were similar in terms of lipid content and cell mass (Fig 2). IPM32-16, ISM28-8sT, and IPM46-17 yielded lipids to >20% dry cell weight and were thus oleaginous species . C. slooffiae JCM 10954T yielded lipids to only 15% dry cell weight. Sugar consumption was proportional to lipid production. However, the sugar-converting efficiency of C. slooffiae JCM 10954T was much lower than those of IPM32-16, ISM28-8sT, and IPM46-17. The sugar yields (g lipid per g sugar) of the three strains were 0.046 to 0.064 g/g, whereas that of C. slooffiae JCM 10954T was 0.03 g/g. Thus, it seems that the strains used different metabolic pathways for lipid production. Nutrients influence lipid production efficiencies because cells use different metabolic pathways depending on the availability of different medium components. A thorough metabolic analysis is required to define the optimal medium for lipid production.
(a) IPM32-16; (b) ISM28-8sT; (c) IPM46-17; (d) C. slooffiae JCM 10954T. Data are means ± standard deviation (error bars) of three replicates. Some errors are very small and hidden by the symbols.
The principal fatty acid in the new strains was oleic acid (C18:1), accounting for 36.3 to 52.8% of all fatty acids in IPM32-16, ISM28-8sT, and IPM46-17, whereas the principal fatty acid of C. slooffiae JCM 10954T was linoleic acid (C18:2) (50.0%) (Table 3, S1 Table for details).
Simultaneous conversion of glucose and xylose is desirable because xylose is one of the most abundant carbohydrates in plants. Hu et al.  investigated the ability of Trichosporon cutaneum (present name, Cutaneotrichosporon cutaneum) AS 2.571 to produce lipids during cultivation on detoxified corn stover hydrolysate. The strain accumulated lipids to 39.2% of dry cell weight. In another study, Lipomyces starkeyi DSM 70296 produced lipids during conversion of a glucose/xylose mixture, to a final content of 27.7% . Huang et al.  found that detoxified rice straw hydrolysate could be used for lipid production by Trichosporon fermentans (present name, Geotrichum fermentans) CICC 1368 (to a lipid content of 23%). When Trichosporon cutaneum (present name, Cutaneotrichosporon cutaneum) CX1 was cultivated with detoxified corn stover hydrolysate, the final lipid content was 23.5% . The published data on lipid production during growth on a mixture of glucose and xylose are shown in Table 4. We did not observe diauxic growth during cultivation of IPM32-16, ISM28-8sT and IPM46-17 on GX medium. The ability to simultaneously utilize multiple sugars to accumulate lipids is of great importance when planning lipid production from lignocellulosic hydrolysates.
The D1/D2 sequences of ISM28-8sT differed from those of C. fimetarium, C. minutum, and C. slooffiae by 8–9 nucleotides (Fig 1), suggesting that our isolates constituted a new species. Based on the sequence of the ITS region, C. slooffiae is more closely related to C. iriomotense ISM28-8sT than are C. fimetarium and C. minutum. Therefore, C. slooffiae JCM 10954 was used as a control strain in the conversion tests, given that the strain accumulates lipids  and can use xylose . Indeed, C. slooffiae JCM 10954T also utilized the sugars simultaneously, but exhibited low-level sugar assimilation and poor lipid production. Interestingly, the three strains preferentially utilized xylose, not glucose (Fig 2A–2C). Generally, transport affinity for glucose is two orders of magnitude higher than that for xylose . However, the sugar transporters of C. iriomotense remain unknown; further work is thus necessary.
IPM32-16, ISM28-8sT and IPM46-17 accumulated lipids rapidly over the initial 4 d of conversion (Fig 2). For example, the lipid productivity of IPM32-16 (Fig 2A) on d 4 was 0.21 g/L/d, and that over the next 6 d 0.013 g/L/d. After 4 days of conversion, much more xylose than glucose was consumed; the consumption rates were 2.10 g/L/d and 1.21 g/L/d, respectively. From d 4 to d 10, the lipid concentration increased slightly as glucose consumption increased. IPM46-17 behaved similarly, indicating that xylose was efficiently used for lipid production. A similar conclusion was reached in a study on lipid production by Cutaneotrichosporon curvata (formerly Cryptococcus curvatus, Candida curvata) using five different carbon sources: glucose, sucrose, lactose, xylose and ethanol . Fig 3 shows that the final lipid level was 1.23 g/L. In the best practical scenario, the lipid concentration attained 4.4 g/L [9, 43]. Our lipid yield was thus low, probably because we did not consider nitrogen limitation, although nitrogen depletion can induce lipid production. The nitrogen balance and other conditions should be optimized in future.
The fatty acid composition of C. minutum cultivated on a medium containing 10 g/L glucose was 62% oleic acid (C18:1), 18% palmitic acid (C16:0), 16% linoleic acid (C18:2), and 4% stearic acid (C18:0) . The lipids contained large amounts of C16 and C18 fatty acids (97.4–97.8% of the totals); the lipid mixtures produced were suitable for biodiesel production . Notably, the fatty acid compositions differed among the strains tested. In terms of stearic acid, the lowest level was 5.7% for C. slooffiae JCM 10954T, but ISM28-8sT had a stearic acid content of 25.1%.
We introduce a new yeast species facilitating efficient lipid production; the strain exhibits a unique metabolic profile. Our species may allow for engineering of xylose metabolism in other oleaginous microorganisms. Further analysis and metabolic characterization may aid in the construction of strains efficiently producing lipids from lignocellulosic biomass. Lipid production experiments using a lignocellulosic hydrolysate, such as rice straw, are required.
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Description of Cystobasidium iriomotense Tanimura, Sugita et Takashima f.a. sp. nov.
Etymology: the name iriomotense (iri.omot.en’se N.L. adj. iriomote pertaining to Iriomote) was derived from “Iriomote Island”, because the type strain of the species was isolated from soil collected on Iriomote Island.
After 3 days at 25°C in YM broth, the cells are subglobose, oval, ellipsoidal (2.5–5) × (5–10) μm, single, or in pairs. A sediment is formed. After 1 month at 17°C, an incomplete and fragile ring and a sediment are produced. After 1 month at 17°C on YM agar, the streak culture is pastel red, smooth, semi-shiny, and soft to butyrous, with fluid near the bottom, and has an entire margin. Neither mycelium nor pseudomycelium form on YM or cornmeal agar. After 4 weeks of incubation on cornmeal agar at room temperature, ballistoconidia are not produced. Sexual reproduction is not observed on YM or cornmeal agar. Does not ferment glucose. Assimilates glucose, galactose (or latent), L-sorbose (latent and weak), sucrose, cellobiose, trehalose, lactose (or latent), melezitose, ethanol (latent), D-xylose, L-arabinose (or latent), D-arabinose (latent and weak), glycerol, ribitol (latent or latent and weak), D-mannitol (latent), D-glucitol (latent), glucono-δ-lactone (or latent), salicin (variable), succinic acid (latent), D-gluconate, D-glucurono-δ-lactone (latent), propane 1,2 diol, N-acetyl-D-glucosamine, saccharic acid (latent), and xylo-oligosaccharide. Does not assimilate maltose, melibiose, raffinose, inulin, soluble starch, D-ribose, L-rhamnose, erythritol, galactitol, methyl-α-D-glucoside, DL-lactic acid, citric acid, inositol, D-glucuronic acid, D-galacturonic acid, methanol, D-glucosamine, L-arabinitol, quinic acid, xylitol, or butane 2, 3 diol. Assimilates lysine hydrochloride. Does not assimilate sodium nitrite, potassium nitrate, ethylamine hydrochloride, or cadaverine dihydrochloride. Requires p- aminobenzoic acid and thiamine for growth. Simultaneously converts glucose and xylose to lipid simultaneously. Grows at 30°C, with variable growth at 35°C and 37°C, but does not grow at 40°C. Starch-like substances are not produced. The diazonium blue B reaction is positive.
Type strain: ISM28-8sT (= JCM 24594T = CBS 15015T), isolated from soil collected in November 2008 at Iriomote Island in the Iriomote Ishigaki National Park, Okinawa, Japan, by T. Sugita. A culture from the holotype strain of this species has been deposited and preserved in a metabolically inactive state in the Japan Collection of Microorganisms (JCM), RIKEN BioResource Research Center, Tsukuba, Ibaraki Prefecture, Japan; and the Centraalbureau voor Schimmelcultures (CBS), Westerdijk Fungal Biodiversity Institute, Utrecht, the Netherlands.
The Mycobank Number is MB 819779.
S1 Table. GC data.
GC data of three separate experiments with the relative standard deviation.
The authors are grateful to the anonymous reviewers for their constructive criticisms of this research.
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- 33. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Anton Leeuw. 1998;73:331–371.
- 34. Fell JW, Boekhout T, Fonseca A, Scorzetti G, Statzell-Tallman A. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol. 2000;50:1351–1371. pmid:10843082
- 35. Scorzetti G, Fell JW, Fonseca A, Statzell-Tallman A. Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res. 2002;2:495–517. pmid:12702266
Sampaio JP, Oberwinkler F. Cystobasidium (Lagerheim) Neuhoff (1924). Yeasts: A Taxonomic Study, Vols 1–3, 5th Edition. 2011:1419–1422.
- 37. Yurkov AM, Kachalkin AV, Daniel HM, Groenewald M, Libkind D, de Garcia V, et al. Two yeast species Cystobasidium psychroaquaticum f.a. sp nov and Cystobasidium rietchieii f.a. sp nov isolated from natural environments, and the transfer of Rhodotorula minuta clade members to the genus Cystobasidium. Anton Leeuw Int J G. 2015;107:173–185.
- 38. Meng X, Yang J, Xu X, Zhang L, Nie Q, Xian M. Biodiesel production from oleaginous microorganisms. Renew Energ. 2009;34:1–5.
- 39. Huang C, Zong M-h, Wu H, Liu Q-p. Microbial oil production from rice straw hydrolysate by Trichosporon fermentans. Bioresour Technol. 2009;100:4535–4538. pmid:19433350
- 40. Farwick A, Bruder S, Schadeweg V, Oreb M, Boles E. Engineering of yeast hexose transporters to transport D-xylose without inhibition by D-glucose. Proc Natl Acad Sci USA. 2014;111:5159–5164. pmid:24706835
- 41. Huang X, Wang YM, Liu W, Bao J. Biological removal of inhibitors leads to the improved lipid production in the lipid fermentation of corn stover hydrolysate by Trichosporon cutaneum. Bioresour Technol. 2011;102:9705–9709. pmid:21880481
- 42. Evans CT, Ratledge C. A comparison of the oleaginous yeast, Candida curvata, grown on different carbon sources in continuous and batch culture. Lipids. 1983;18:623–629. pmid:6633167
- 43. Bommareddy RR, Sabra W, Maheshwari G, Zeng AP. Metabolic network analysis and experimental study of lipid production in Rhodosporidium toruloides grown on single and mixed substrates. Microb Cell Fact. 2015;14.
- 44. Sargeant LA, Mardell M, Saad-Allah KM, Hussein AH, Whiffin F, Santomauro F, et al. Production of lipid from depolymerised lignocellulose using the biocontrol yeast, Rhodotorula minuta: The fatty acid profile remains stable irrespective of environmental conditions. Eur J Lipid Sci Tech. 2016;118:777–787.
- 45. Knothe G. Improving biodiesel fuel properties by modifying fatty ester composition. Energ Environ Sci. 2009;2:759–766.


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Mexico is a land rich with archaeological wonders of the world, lively music and unforgettable festivities. “Barriga llena, corazón content” (full belly, happy heart)…. You will be amazed by the heaping platters of fabulous robust flavors – fresh seafood, “dry soups”, mole, barbacoa, carnitas and mixiotes! Explore the unique regional preparations – Corundas of Michoacán (a triangular tamale cake variant), the Pepita con Tasajo (strips of salt-cured beef in a sauce of ground pumpkin seeds and spices), and Huachinango à la Veracruzana (red snapper prepared in lime juice, jalepenos, olives, and raisins).
Want to hike along some of the most gorgeous scenes in Mexico? Perch along the rocky cliffs and ancient ruined walls of Tamul overlooking the Caribbean. Horseback ride or hike along the spectacular Copper Canyon, four times larger than its American counterpart, with its deep chasms, waterfall gorges, and thrilling heights. Let Travel Benefits by Design lead you through the adventurous discoveries and fascinating culture of Mexico.
Mexico City represents a blend of Mexican culture and thriving commerce. Immerse yourself with the music of the Mariachi at the Garibaldi Plaza and imbibe at Museo del Tequila y el Mezcal’s drinking tours.The Master Concert & Performing Arts Center (Palacio de Bellas Artes) and the National Palace are Mexico City’s classical landmarks. The National Palace contains three interior courtyards, nine Diego Riviera murals depicting native life before the Spanish Conquistadors, and the historic bell tower of Campana de Dolores, which rings in the War of Independence celebration to this present day. Learn about the Hispanic golden age of revolutionary art and muralists by visiting the Museo Dolores Olmedo Patino, featuring the works of Diego Riviera and Frida Kahlo. An absolute “sight-to-see” is the resplendent opulence of Castillo de Chapultepec. It houses the palatial residence of Emperor Maximilian and Empress Carlota, a museum depicting Mexican history, the WWII Tribuna Monumental de las Águilas and the state gardens.
Other City Highlights
Visit the city of Taxco’s Santa Prisca, a heavily ornate Baroque cathedral filled with some of the finest gold inlay interiors in Mexico. Spend a few days at Guanajuato, the most beautiful of Mexico’s silver mining cities.
Guanajuato is filled with charming colonial buildings of pink and green limestone and ornate neoclassical architecture (the Juarez Theater, the Oratorio de San Felipe, and the University of Guanajuato). The Museo del Pueblo de Guanajuato houses the famous artwork of Olga Costa and José Chávez Morado. Another unique characteristic of this city are its winding alleys and cobbled streets atop mountainsides and its massive archways both above ground and below ground, supporting a labyrinth of subterranean roads.
Mexico’s Archaelogical Heritage
Visit the City of the Gods at the archaeological site of Teotihuacan. The mysterious origins of Teotihuacan remain unknown, and even the powerful Aztecs who later discovered its ruins, would be in complete awe, giving it the name “Birthplace of the Gods”. Stroll down Teotihuacan’s pyramid-lined Avenue of the Dead, from the immense Pyramid of the Sun to the Pyramid of the Moon. Some of the most lovely archaeological artworks unearthed are gold and jade decorated carvings, wonderfully preserved murals of scarlet and gold, marble and granite structures, and masks of obsidian. Indeed Teotihuacan was a central hub for royal-commissioned artisans and master craftsmen gathered from all over the ancient world.
Mexico’s Chichen Itza is the largest and the best restored of the Yucatán Maya sites and the most important city of the Mayan ancient civilization. The Great North Platform includes the monuments of El Castillo, the Temple of Warriors and the Great Ball Court braced by the fearsome Jaguar emblems and almost gargoyle-like mosaic masks of primitive deities. The El Castillo houses an incredible convergence of mathematical configurations – 52 panels for 52 year solar cycles, 91 steps and platform equating to 365 days of the year, and 9 terraces divided to the months of the Mayan calendar. At the outskirts of Chichen Itza are the sacred caves of Balankanche and the ominous Cenote Segrado where sacrificial remains have been found.
March 1, 2016 6:03 pm

domain: artistic


text:
BCBG Max Azria Jacket WTI8B560 - Treat yourself to a stylish classic in this BCBG Max Azria jacket.
- 100% wool.
- Classic mid-thigh length hem.
- Center seam at back with back vent.
Weather and Wind:
- Seamed flap pockets at front.
- Three-quarter length sleeves with triple-button accents at cuffs.
- Dry clean only.
- Double-breasted button-up closure at front.

domain: design


text:
How Hollywood Hills residents plan to keep developers out: ‘Let’s Buy a Mountain’
To keep a mountainous stretch of the Hollywood Hills out of the hands of real estate developers, an alliance of Laurel Canyon residents and wildlife advocates is raising money to buy it.
“Please help us buy this mountain,” Jamie Hall, president of the Laurel Canyon Assn., pleaded to an array of television cameras Wednesday atop the hills. “It’s not as crazy as you think.”
The Laurel Canyon Assn., Citizens for Los Angeles Wildlife and their allies kicked off their efforts with a news conference at a show-stopping crest of the rugged property.
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Their campaign, dubbed “Let’s Buy a Mountain,” was the brainchild of Hall, a land-use attorney and local resident who said he was sick of battling new development after it was already in the works.
Frustration with development has simmered in the Hollywood Hills, where neighborhood groups have complained that the pace of new housing construction and other development has not been matched by the infrastructure to support it. Such complaints were a driving force in the recent City Council election that ushered in City Hall outsider David Ryu to represent a sprawling district that includes Laurel Canyon.
In Laurel Canyon, residents have been worried about losing the rustic feel of the storied, sylvan area known for inspiring poets, artists and musicians like Joni Mitchell. “I always get embroiled in these one-off disputes — this home, that home. It felt very reactionary,” Hall said. When he and his neighbors found out that an undeveloped swath of the mountains could be up for grabs, “we wanted to do this a different way.”
Instead of battling future plans to develop the hillside, residents decided to band together to raise $1.6 million, the going price for the 17-acre chunk of L.A.’s urban wilderness.
The Laurel Canyon ridge they want to buy stretches between Lookout Mountain Avenue and Stanley Hills Drive, an area dotted with oaks and home to bobcats, coyotes and deer. Environmental advocates say it is a crucial corridor for big mammals in the Hollywood Hills.
“We’re watching these incredible green spaces disappear very quickly,” said Alison Simard, founder and chair of Citizens for Los Angeles Wildlife. “We need to grab this land where we can and preserve it.”
If they succeed in buying the land, it would be turned over to the Mountains Recreation and Conservation Authority, a local government agency that manages open land, to be preserved as green space. The Laurel Canyon Land Trust, a newly formed group, would retain the rights to prevent future development on the mountain, Hall said.
Under an agreement with the current owners, the groups have a year and a half to come up with the money. They must first drum up $50,000 to put down a deposit by early November.
“This is an opportunity that comes once in a lifetime,” Councilman Ryu said at the news conference. He urged Angelenos to donate to the cause. “Please donate today so this important piece of L.A.’s green belt will be forever removed from the threat of development.”
Simard said the alliance had raised $20,000 as of midday Wednesday. Ryu has also pledged to match up to $15,000 of any additional funds raised to reach that November goal. The money will come from fees charged to area developers that are designated for park funding, according to his office.
Follow @LATimesEmily for breaking news from Los Angeles City Hall.
The stories shaping California
Get up to speed with our Essential California newsletter, sent six days a week.
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domain: news


text:
A social media client wanted to understand the challenges local business owners face when they begin using online marketing tools to promote their local shops.
WIRe champions diversity in the market research industry with their mission to advance the contributions and voice of women in the industry. Together with Keen as Mustard they the Best Places to Work Award brought to life to honor the organizations which do their best to support their female employees at the workplace.
We are very happy to be among the shortlisted companies considered for the award! We have done a lot in this field and are glad our efforts to push diversity and equality didn't go unnoticed.
Did you know that there are nearly as many women in management positions in Fortune 500 companies as men named John? What sounds like a joke is a sad fact as data from The New York Time's Glass Ceiling Index proves. And that is not all, even though many companies claim that they are highly committed to diversity this hasn't translated into numbers as they remain nearly the same year after year. So, it comes as no surprise that Germany and the USA ranked 21st and 20th regarding the environment of working women in The Economist's latest Class Ceiling index.
However, Factworks commits itself to equal opportunities and wants to captivate its employees no matter which gender. Our goal is to help everyone live up to their full potential and become outstanding market researchers!
And our numbers speak for themselves:
More than 2 in 3 of our team members are female. Our 2 CEO positions are equally shared between a man and woman, and all of our team leading roles are filled by women. Also, we do our best to create attractive conditions for young parents: 40% of our female workers are also mothers with one or more children and taking both male and female employees into consideration, 37% of the team are parents. We provide flexible working hours, work from home options and several more perks to cater to their needs.

domain: sociology


text:
This blog recently discussed tips for a covered entity (CE) in dealing with a HIPAA business associate (BA). Now, even though you have adopted all of the tips and more, in this dangerous and ever more complex data security world, one of your BAs suffers a breach and it becomes your responsibility as the victim CE to respond. What should you do?
Our partner Elizabeth Litten and I discussed aspects of this issue with our good friend Marla Durben Hirsch who included some of our discussion in her article in the June 2017 issue of Medical Practice Compliance Alert entitled “6 ways practices can reduce the risk of delegating breach-notification duties.” Full text of the article can be found in the June, 2017 issue, but a number of the items included below are drawn from the article.
- Locate the most recent Business Associate Agreement (BAA) with the BA who experienced the breach, and see what it says about the post-breach obligations of the CE and the BA. Two important threshold issues are whether the BA complied with the time period for reporting breaches to the CE contained in the BAA and the remaining time, if any, available to the CE for complying with any reporting requirements under HIPAA and state law, remediation and limitation of loss requirements, and notification requirements to affected individuals (collectively, the Requirements).
- Determine promptly what are the time deadlines for notification to insurance carriers if cybersecurity or general liability insurance may be available to the BA and/or the CE for payment of expenses of the breach and its remediation.
- Spell out any circumstances where the BA will handle the consequences of a breach that occurred on its watch, and the scope of its responsibilities vs. that of the CE. These can range from delegating to the BA the entire range of Requirements to assumption by the CE of complying with the Requirements with payment by the BA of the costs thereof.
- Make sure that the required reporting and notification Requirements are sent on CE stationery or, if such Requirements are being delegated to the BA (especially where the breach affected a number of different CEs), the notifications make it clear that the breach was attributable to the acts of the BA and not the CE. As CE, insist that the final wording of the required reporting and notification documents be subject to your approval.
- Ensure that your staff is familiar with the circumstances of the breach so that they will be able to answer questions from affected individuals and the media intelligently. It may be advisable to designate a single trained and articulate person to be referred all inquiries, so that the responses are uniform, accurate and clear.
- Assess whether the BA handled the breach adequately and whether you want to retain your relationship with the BA. Did the BA comply with HIPAA and the BAA in the post-breach period? Did the BA cooperate with the CE? What is the likelihood of a repeat breach by the BA? Is the CE assuming the risk of potential repeat HIPAA breaches if the BA relationship is continued?
- If you determine as CE that you will continue your relationship with the breaching BA, consider whether the BAA with the BA requires changes based upon the experience of the breach and its aftermath.
- As CE, consider modifying, updating and/or strengthening all of your BAAs as a result of your experience.
- As CE, you may require improving and/or changing your cybersecurity insurance coverage as a result of experience with the breach.
- As CE, document all activities and decisions respecting HIPAA made in the post-breach period to defend your actions as reasonable and to provide concrete planning steps for future HIPAA compliance.
While all the precautions in the universe by a CE cannot eliminate a HIPAA breach by a BA, a CE that is victimized by such a HIPAA breach can do many things to reduce its liability and image damage and strengthen its own HIPAA compliance and risk avoidance efforts for the future by adopting the steps described above.

domain: law


text:
Skip to Main Content
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Texas General Land Office Historic Map Collection
More than 50,000 maps, sketches, and documents dating from as early as the 1820s. There are manuscript and printed maps showing Texas, its counties, cities, railroad routes, colonies, forts, Indian villages, large ranches, waterways, and other features
Portal to Texas History
Over 500 maps of Texas, including county soil survey maps of the early 1900s. These may be enlarged and manipulated. On the search screen limit to Maps.
Library of Congress--Civil War Maps
Brings together materials from three premier collections: the Library of Congress Geography and Map Division, the Virginia Historical Society, and the Library of Virginia. Among the reconnaissance, sketch, and theater-of-war maps are the detailed battle maps made by Major Jedediah Hotchkiss for Generals Lee and Jackson, General Sherman's Southern military campaigns, and maps taken from diaries, scrapbooks, and manuscripts all available for the first time in one place.
Cartographic Connections--UT Arlington
76 historic maps from the UTA collection, many focusing on Texas. The map collection is temporarily down, but this link provides contact information.
Atlas of The Official Records of the War of the Rebellion
All of the plates in the atlas that accompanies the official documentary history of the American Civil War.
Maps at the Texas State Library
A searchable database to descriptions of over 8,000 maps. Digital images of some 700 maps are also accessible through this database.
Perry-Castañeda Library Historical Map Collection (UT-Austin)
Vast collection of online historical, climatic, population, industrial, military, and other maps.
Texas Historic Sites Atlas
Nearly 300,000 site records, including data on official Texas historical markers and National Register of Historic Places properties, courthouses, museums, and sawmills. Includes map links.
Atlases and Maps in the UT-Tyler Library
The UT Tyler Library owns a complete set of Texas topographical maps which are mounted but uncataloged, located downstairs in the University Archives and Special Collections.
Atlases are housed on the 3rd floor of the library.
, Criminal Justice
, Health Care Administration
, Political Science
, Public Administration
, Social Sciences
, Social Work

domain: history


text:
Fri-end-ship.Fri-end-ship.Fri-end-ship.2 years ago in Urban & Spoken Word More Like This
When I talk, you don’t listen.
You’re just waiting for your turn to speak.
You view every conversation as a competition.
All I can see is the unstoppable movement of your cheeks.
The flow of communication is always re directed back to you.
Almost as if everyone must hear what you have to say.
I’m not denying that half of what you say could actually be true.
But how can so much happen to a person in just one mere day.
What makes it worse is that your stories get recycled and repeated.
I zone in and out of your speeches and know exactly what happens next.
My place in the conversation is to respond and nod when needed.
If you can’t tell me in person, you incessantly try to call me or even text.
I can feel my tolerance and patience gradually wearing thin.
I am not even sure how much longer I can hold it in.
I want to tell you the truth but I don’t know how to phrase it.
Because I know once it is delivered, it is impossible to erase it.
So I have
MentalityA trick of the mind is all it takesMentality2 years ago in Free Verse More Like This
To make you make some big mistakes.
Depression Is NotNaïve child,Depression Is Not3 years ago in Free Verse More Like This
Depression is not
A brief glimpse of
Falling to a foreseeable bottom.
It is not being driven
To the underbelly of Hell
And burned on a stake
By those persecutors.
Depression is not
Simple statements of
Self-pity cooked together
With endearment of a knife
You have just met.
It is not
Half-hearted suicide attempts
Filled with attention seeking.
It is not
A brief glimpse of sadness
Because someone dislikes you.
Has no conceivable stop
It is not a self-inflicted wound,
But a wound that causes
It is no glimpse,
It is not the cause
As it hides you away
From people who love you.
So take no claim to it
If sadness is what you have;
Embrace it, as you are lucky.
Depression is not you.
Stepping Stone FriendI am what you call a stepping stone friend,Stepping Stone Friend3 years ago in Free Verse More Like This
I don't really matter to you,
You just use me,
Take advantage of my friends,
And turn them against me,
I did not know I was being used,
But, you were using me.
I was just your stepping stone.
You did not care about me.
You took my reputation,
Stole my friends,
And turned the world against me.
You used me to push yourself higher.
You say this is all I can do,
Be a stepping stone.
According to you I have no feelings,
I am nothing,
But a stepping stone.
It is not enough to writeIt is not enough to put the words on pageIt is not enough to write3 years ago in Free Verse More Like This
or to align them like cocaine lines
in neat rows of cornstalk paragraphs
fertile enough to bear reviews.
No. One must bleed each period,
each dot-dot-dot like morse code mythology
the Gallic cry at the end of the telegraph age.
It must become an ocean in you, these voices
swelling to tidal highs, and quiet - never.
You the new folkteller, urban prophet
who can call to battle anyone with eyes.
Ooze it like sap spilling down the bark.
It is not enough to write.
One must expire with each keystroke,
endlessly. It must come from the bowels.
Purge it as infection leaking out of skin;
lance yourself. Choke back tears.
If there is no labor pain,
the words were never born.
This is a death business.
We bleed ourselves onto paper and
slice our brains into vellum sheet
and repeat, repeat, repeat.
Pure person petrichor
deep inside the ink.
And That's Wrong.You're fat.And That's Wrong.2 years ago in Free Verse More Like This
Or maybe you're
I don't know.
But you're not perfect.
And that's wrong.
Or maybe you're
But you're not perfect.
And that's wrong.
Or maybe you're
But you're not perfect.
And that's wrong.
Or maybe you're
like everybody else?
You're not perfect.
And that's wrong.
The Children of AmericaThe Children of AmericaThe Children of America3 years ago in Short Stories More Like This
Music that really helped me get my emotions and words straight. I beg of you to listen to it while reading.
For once, the Hero could only stand back in horror at what was going on around him. Screams of terror, wails of agony, names yelled out to every corner of the town, as mothers and fathers and siblings fought back tears at the knowledge of what had happened, and pushing down their fears that they will not see their beloved children again.
To hold, caress, or love....To tell stories to, or to comfort in the night. Their hearts were breaking as the blonde man could only shake. A crack had appeared in his glasses, blurring the foreground before him, trying to warap his mind around the meer information that spilled across the yellow taped lines.
"Why....?" He muttered, his voice lost amoung the sirens, barking, the commanding shouts, and the cries of misery at what had happened. "Why?" He questioned again, stumbl
Am I Crazy?Am I Crazy?3 years ago in Free Verse More Like This
Was I crazy to think
That I could have
Every part of you?
Wishing to be in your arms
That'd your heart beat for me
And I'd be the one you'd lay your eyes on
I was always insane
Loosing my grip
Changing slowly everyday
Where has my sanity wandered off to?
I know for a fact
I can't have you without it.
This lifetime search
For something so trivial
Has ruined it all for me
I have wasted the years
Shedding the tears
All because you never wanted me
I'm Fine"Are you okay?"I'm Fine2 years ago in Free Verse More Like This
No. I'm dying. I have to push myself to wake up in the morning, and when I finally do, I want to go back to sleep. Even my best dreams are becoming nightmares. I can't taste food, I can't stand the things I used to love. I'm breaking. I'm fading. I'm dying.
I Am NosferatuI Am Nosferatu2 years ago in Free Verse More Like This
And he rose like a dream shrouded in obsidian
“Blood the sky!” – bewailed ravenous eyes ..
‘Upon a world solemn; and the dead betrothed
Where lust a river dark, my ache roams wild
Many moons spent wither’d in winds dreary;
naked in breath of whispered-flame & wine
O’ darkly I hear thee my beloved enchant,
thy seraphic-tongue dripping in Night’s mist
Thou art the evening tide flooding my soul,
— gossamer-wings of a gothic serenade ...
Seek you mine lips in a song of shadows
For in immortal sin we shall not wane
Unto a sea of crimson, my love hath assail’d
From Earth to tomb, I am death & hallowed
— Arthur Crow © 2013
The Slaughtered Children.Why? Why? They were children! Children! How could someone strip a child, multiple children, of their innocence! How could someone strip them of life?The Slaughtered Children.3 years ago in Free Verse More Like This
Children are one of the few good things in life. Always learning, not dispicable liars or haters or cheaters like the majority of adults, not able to commit horrendous crimes, not aware of the greater scheme of life going on around them. Believer's they are. In a child's mind, the characters of stories such as Santa Clause and the Easter Bunny are alive. Magic is real and all around us, commanded and cast by the fairies who were blessed by the mighty kings and queens from far far away. In a childs dream, there is no deception, no hatred, no confusion. There is only light and happiness and love, and their imagination is never as strong as it if in childhood.
It is for those reasons, and many more, why I believe children should be treasured. For their beautifully bliss minds and perfectly balanced ignorance. For their imagination, raw and i
Ashen Sky-Chapter 15 RevisedA day and a half passed inside the Museum of Science and Industry with no further oddities presented by the other group. Dr. Oliver only let anyone enter his lab if he needed help with something. He only let Scott out to use the bathroom that was until he slipped into a deep sleep.Ashen Sky-Chapter 15 Revised3 years ago in Introductions & Chapters More Like This
"It's the beginning of the end for him, I fear," Dr. Oliver said softly after he had called Matt, Tess, Riley, and Sara into his lab. He sounded mournful, though Matt could almost guarantee it was only because he was losing his test subject. "The initial sickness has passed. He will wake unaware that he was ever ill. After that he will turn quickly into a monster. Before I left the UIC labs, we discovered that the longer the first stage is drawn out, the quicker the second stage progresses, so my best guess is that we have maybe hours."
"Shouldn't we you know " Matt began, though he could not finish his sentence as his eyes fell on Sara, who's own had had not left the sleeping Scott
The chosen: Chapter OneThe chosen: Chapter One4 years ago in Short Stories More Like This
Train En Route to Ponyville - 5:15 AM
It would take about four hours to get from Manehattan to Ponyville. I wanted to use the time to catch up on some much needed rest, but despite my best efforts sleep was just something my body refused. I spent an hour staring out the window before I decided to do something else instead.
It was about time I caught up on the newest issue of "The Adventures of Captain Baseball Bat Boy", an import graphic novel from the human federation and still my favorite graphic novel since foalhood. It mostly dealt with a hyperactive human kid that solved his problems by cracking heads with his baseball bat. In this month's issue, Captain Baseball Bat Boy was up against his most dangerous nemesis; the nefarious Maxwell's Demon and his army, The Freaking Demons from Outer Space. It was good stuff that made the last leg of the trip bearable. Still, it wasn't that long and I finished it in about a half an hour.
It was a guilty pleasure, and one that at least kept my h
I'm taking whatever it isI'm taking whatever it is that they chose for me.I'm taking whatever it is3 years ago in Traditional Fixed Forms More Like This
Though, justice in this, I can't comprehend.
But I can just laugh in the face of absurdity.
A good sense of humour - the best of my friends.
A Poem For YouDear you,A Poem For You3 years ago in Free Verse More Like This
If I could tell you
Of this poetic emotive
Life I lived with our
I would have written you this
Many years ago:
Despite my youth,
It takes a world of disdain
For you to heed
My outward prayers of no.
I tip my world
To hide underneath it
Shrouding away from the
Secret we hold in our bosoms
Splintered by your hands.
I taste the poison
Of lips I think I have once held
But uncertainty reigns
My once sharp mind.
I disguise the now poisonous
Memories we hold together
Yet type of how we have
Slept of nights in terror
Of ourselves and one another.
We have been transfixed
In hatred and blood,
Destined to ruin ourselves
By a touch of one another.
You dishevel your once
Serene life and I wander off
After a knife that shows
The sparkle of my smile as
I hold onto recklessness
In this chaotic life.
And years have passed
Since that fateful day,
In a basement filled
With shadows you emerged into.
And as you bound
my hands together,
You unraveled my mind.
You will fall
Ashen Sky-Ch.1 Revised"I always knew the zombie apocalypse would start in Chicago. I just never thought the zombies would be trying to buy life insurance." Matt Owens chuckled as he glanced over his shoulder to his cubicle-mate.Ashen Sky-Ch.1 Revised4 years ago in Introductions & Chapters More Like This
Tess Abernathy rolled her large blue eyes at him and sighed. "As much as I absolutely adore my job in data-entry here," she began, her voice dripping with sarcasm, "I love your amazing ability to come up with the stupidest ideas ever even more."
"Aw, c'mon, Tess!" said Matt, laughing. "Seriously, though. I had to look this guy up 'cause his address was wrong, and the database says he died at an old address about a month ago, but he's alive at a new address now. Weird, isn't it?"
Tess rolled her eyes again. This was common practice when it came to Matt. "He was probably trying to jump rent or evade taxes or something," she dismissed, glancing at Matt's work before frowning and turning back to her own pair of screens. "Matt, you're doing that thing with your finger again. Would
The PullWhen I was younger, someone showed me a video gametoo weird for me, but it made her laugh, and she was pretty. You played as this little guy with a squishy hammer for a head, and you rolled a sticky ball around in front of you. As you rolled it, things got stuck until the ball was gigantic. And then... I don't know. I don't remember the point of the game, nor do I remember the name.The Pull3 years ago in Short Stories More Like This
But that image comes back to me every time I am anxious. I am that little person running around, pushing a ball, and things stick to it. Only they aren't cows or trees or parts of buildings: they are things that make me nervous. The attention of people. My sparse resume. The way I can never look someone in the eye when we first meet.
Oh. And I don't have a squishy hammer for a head.
Regardless, today is like that. I've talked to too many people and some weird man had told me he was my father and my mother was on the back of a book with a different name but the same damn face.
While I was walking home,
Fear of FlyingThe minute he stepped off the planeFear of Flying3 years ago in Free Verse More Like This
he thought of her and how she hated
flying - how the white metal protruded
and streamlined around her,
anxious to be friends;
how the engine gasped in awe
as it buckled the weight of 234
strangers and kept them from floating
She was afraid of crashing and of caring
what clothes she packed in matching
luggage that would not make it home.
She hated the folding trays,
how they all stood at attention
and the smell of mint souring the stewardess'
breath as she leaned in too far with a pack of
He reminded her that the wings were
sturdy girders, not made of balsa wood and floss
like the ones at the museum and that
the drone was some sort of science -
not some strange addiction
or a scheme of addled magic bumping against the
and that falling always took
the wrong kind of courage.
Rin's Kokoro in Story form :3Rin's Kokoro in Story form :34 years ago in Songs & Lyrics More Like This
I was made by a very lonely scientist. I was the miracle robot. But I was missing one thing. A program that my professor didn't have the ability to make,
Many years passed. I was left all alone. I made a wish. I wanted that wish to come true very badly. I wished for the kokoro made by that person. The person I've wanted to know my entire life. The man who worked on the kokoro until his life ended. Now that mi
Girl as ColorColor the girl shell pinkGirl as Color3 years ago in Free Verse More Like This
or magnolia -
some rare vintage of wine
or a bright Italian circus,
and I shall call her mine.
Color her starling grey
or a sudden shade
daylight glistening like a puzzle
and I will claim her mouth.
Paint us both some new corner
a fresh tone of orchestra
and ripened lemons,
and I swear I will be true.
Bipolar DisorderShut up.Bipolar Disorder2 years ago in Free Verse More Like This
Don’t. Say. A n y t h i n g .
“Are you okay?”
P an i c .
Close the window.
Open it again.
“What’s wrong with you?”
I’m losing control.
I am Bipolar Disorder.
… “I don’t know.”
The Lost Timeline Ch. 3The Lost Timeline Ch. 34 years ago in General Fiction More Like This
Homura was in shock the whole walk home. She replayed the events at the cafe in her mind. She remembered Sayaka's energy, how could that all be fake? How could she be covering up such sadness so well? Homura felt sorry for Sayaka. She...must be hurting tremendously... she thought. She unlocked the door to her apartment and flopped into a chair. Her mind was racing. When will the Witch strike? Where is Kyubey in all this? Why haven't I seen him? Homura's head started to hurt so she took some medicine and went to sleep.
Homura woke up in a cold sweat. She had been having a terrible nightmare. "That dream..." In the dream, she and Madoka were separated by a wall. They could see each other, but not touch or hear each other. Madoka was crying and no matter how hard Homura tried, she could not break through the wall and comfort her. She was sobbing, and banging against the wall, but all Homura could do was watch. That was a terrible dream... Homura put her han
You don't seem to notice (my scars)-i-You don't seem to notice (my scars)2 years ago in Stories & Vignettes More Like This
He and I were eleven when we met, the first day of the sixth grade. No particular moment served as the spark to ignite our friendship. As children do, we started talking as if we were already good friends, and were inseparable from the start.
There were rumours, but we didn't understand half of the words the other kids had picked up from R-rated movies, and neither did they. We were called King and Queen by a crowd of boisterous first-graders who followed us around at recess. He joined the choir and the school play just because I did. It didn't take long before we weren't allowed to sit near each other on the school bus because we caused too much trouble, and eventually we weren't allowed to sit near each other in class either. One day, more quietly than I had ever heard him, he asked me to be his girlfriend; I blushed and said yes.
We never once invited each other to our houses. We each had our reasons, but never knew the other's: a silent agreement to n

domain: literature


text:
Deluxe ATV Expedition and Resort Beach
Double Rider: $210.00
Off Road Adventure and Beach Day
Your amazing day of fun and adventure begins with a comfortable ride in an air-conditioned vehicle through the bustling city of Puerto Plata, the historic scenic Victorian District and along the Malecon, before heading through a typical village. Along the way, your guide will share the history of the area and explain what daily life is like for the average family residing here.
Upon arrival to the base camp, you will attend an orientation and safety briefing, then be expertly fitted with your helmet and goggles. Hop on your stable UTV, adjust your bandana, and feel the adrenaline surge as you follow your local guide along back roads, across streams, and through the beautiful Dominican countryside. Your UTV is certainly not your typical or standard ATV; this highly advanced and high-end UTV is nimble and easy to handle, making for a great experience. Your trip will never travel on the main roads, making this a very safe and scenic ride. Take the opportunity to cool off in the incredibly beautiful river pool before heading back to base camp, where you will be treated to a coffee and rum-tasting. You can take a break, relax and just chill out at the base ranch that is fun and entertaining before continuing to your next adventure.
From there you will board your air-conditioned transport for a short ride over to the picturesque palm-lined Playa Dorada Beach. This beautiful beach is famous for its fine, golden sand and clear, turquoise waters. Your exclusive access to Playa Dorada Beach takes you to one of the most famous beaches of the north coast and is preferred by those seeking serenity and beauty. Relax on a complimentary lounger (first come, first serve) and work on your tan or pop into the beach side cafe for a bite or drink (food and drink at your own expense) as the mesmerizing waves roll your cares away. You will find fresh water showers here to rinse off and you will enjoy the atmosphere and the facilities provided that make for a more upscale experience. This is the absolute perfect way to round out your UTV adventure and will be guaranteed to make memories that will last a lifetime and a day you will talk about for years to come.
This popular 4.5-hour tour sells out fast as space is limited! Book today for an exciting adventure on the backroads and beaches of the Dominican Republic that you will fondly remember for years to come.
Please Note: The UTVs available for this tour accommodate up to 2 guests maximum per UTV. Please take this information into consideration when booking.

domain: travel


text:
Riddle me this - why is 5% hydrogen in nitrogen considered NOT flammable and 4% in argon considered flammable? Tried to reach Praxair but no one is home.
This makes absolutely no sense to me.
Debbie M. Decker, CCHO, ACS Fellow
Past Chair, Division of Chemical Health and Safety
Councilor and Programming Co-Chair
University of California, Davis
Birkett's hypothesis: "Any chemical reaction
that proceeds smoothly under normal conditions,
can proceed violently in the presence of an idiot."
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domain: chemistry


text:
Books & Bibles
Fashion & Jewelry
Gifts & Giving
Home Decor & Accents
Kitchen & Gourmet
Beauty & Health
Christian Bookstore .Net is a leading online Christian book store.
Shop Christian Books, Bibles, Jewelry, Church Supplies, Homeschool Curriculum & More!
For the last decade of his life, Evelyn Waugh experienced the changes being made to the Church's liturgy to be nothing short of "a bitter trial." In Cardinal Heenan he found a sympathetic pastor and a kindred spirit. This volume makes available the previously unpublished correspondence between these prominent Catholics, revealing in both an incisive disquiet.
Buy A Bitter Trial: Evelyn Waugh and John Carmel Cardinal Heenan on Liturgical Changes by Evelyn Waugh & John Carmel Cardinal Heenan from our Christian Books store - isbn: 9781901157055 & 1901157059
The team at Christian Bookstore .Net welcome you to our Christian Book store! We offer the best selections of Christian Books, Bibles, Christian Music, Inspirational Jewelry and Clothing, Homeschool curriculum, and Church Supplies. We encourage you to purchase your copy of A Bitter Trial: Evelyn Waugh and John Carmel Cardinal Heenan on Liturgical Changes by Evelyn Waugh & John Carmel Cardinal Heenan today - and if you are for any reason not happy, you have 30 days to return it. Please contact us at 1-877-205-6402 if you have any questions.
Customer Support: 1-888-395-0572


text:
Overview of AfroPulse
AfroPulse hopes to provide a forum through which African students, those of the African Diaspora, and all other interested students can come together to explore the continent in a multi-dimensional context.
Our goals include spreading awareness of the continent’s rich and diverse history and culture to the wider Bard community, as well as celebrating this culture through food, music, film, dance, and fashion. We also hope to educate on the contemporary issues faced by countries on the continent, and shed light on the place of Africa and its people in our world today through panel discussions, documentary/TED talk screenings, live speakers, and debates. Community service will be a priority in our agenda, and we hope to find practical ways to contribute back to communities across Africa. We will also attempt to reach out to the larger Hudson Valley community through community service activities that promote the continent, and by engaging with similar African student groups in surrounding colleges.
Club Officers Marra Garcia


text:
Students of iconoclastic computer scientist Daniel Bernstein have found some 44 security flaws in various Unix applications, according to a list of advisories posted online.
The flaws, which range from minor slipups in rarely used applications to more serious vulnerabilities in software that ships with most versions of the Linux operating system, were found as part of Bernstein's graduate-level course at the University of Illinois at Chicago.
"Every program is used somewhere--this was a requirement for the homework--but the programs vary widely in popularity," Bernstein, a professor of computer science at the university, stated in an e-mail interview Thursday.
The advisories regarding the flaws were dated Wednesday and can be found on the
Web site of student James Longstreet


As in nearly every previous year, Whipple Superchargers is first to the market again with an incredible bolt-on Mustang supercharger system. The formidable team of Whipple Superchargers and Ford Racing has again set a new precedence for bolt-on power and industry leading durability.
Nobody puts more time and resources into the development then Whipple and Ford Racing. The proof is in the product, more power per lb of boost then any other system. The key to making such incredible reliable power is in the Whipple unique twin screw supercharger, massive air-to-water intercooler, oversized Crusher air system and innovative PCM programming that keeps the power-train safe and secure. Whether youre looking for a daily driver that needs a little more punch or youre craving a tire-frying, rubber shredding street machine, the Whipple Supercharger Ford Racing systems delivers like no other SC system today. Power from tip in to redline, the new Whipple SC system makes incredible power across the entire rpm range while maintaining industry leading drive-ability.
With years of OEM and racing experience, Whipple and Ford engineers have developed a unique PCM calibration that reconfigures all the necessary engine functions including fuel, spark, knock detection, torque management, transmission control and electronic throttle control. The vehicles PCM constantly monitors the engines torque output while monitoring many different parameters to keep the engine in its ideal parameters. If a parameter falls below specification, the PCM will lower boost and torque output until the problem can be cured. No other calibration in the field has such technology, giving the Whipple-FRPP SC system unheralded performance and reliability.
Simple bolt-on system
Instant boost, zero lag time for incredible throttle response
Shelby GT500 factory throttle body
Revolutionary W140ax Gen 2 Whipple twin-screw supercharger
Massive bar-plate aluminum intercooler for superior cooling
Custom 45lb/hr fuel injectors
Twin screw supercharger is the most efficient SC on the market
Self contained oil system, no tapping oil pan
Exclusive high flow factory air box style system
Ford Racing flash programmer for industry leading power, reliability and durability
Zero tuning required, all pre-set
Available for manual transmission equipped vehicles
*Power levels may vary depending on dyno make as well as testing conditions.
MUST CALL FOR PRICING DUE TO THE DIFFERENT VARIABLES THAT COULD EFFECT THE BUILD
This product hasn't received any reviews yet. Be the first to review this product!
All prices are in USD


BAGHDAD, Sept. 12 (Xinhua) -- The Iraqi parliament voted Tuesday to reject the independence referendum of the semi-autonomous Kurdistan region slated for Sept. 25, a parliament source said.
"The majority of lawmakers during the parliament session voted in favor of a draft rejecting the referendum on the independence of Kurdistan region in northern Iraq," the source told Xinhua on condition of anonymity
The Kurdish lawmakers walked out of the session in protest, the source said.
"The draft put obligation on the Prime Minister Haider al-Abadi to take all measures that preserve the unity of Iraq and start a serious dialogue to address outstanding issues between Baghdad and the Kurdish region," the source said.
Hoshyar Zebari, a Kurdish leading figure and former Iraq's Finance and Foreign Minister, said earlier in an interview with Xinhua that the referendum will be held on Sept. 25, after all the official bodies in the region completed their preparations in all aspects of security and logistics.
On June 7, the Kurdish President Masoud Barzani announced his intention to hold a referendum on the independence of the Kurdish region from Iraq on Sept. 25.
The independence of Kurdistan is expected to be opposed by some countries because it would threaten the integrity of Iraq and because it comes as the Iraqi forces are in fight against terrorism, including the Islamic State (IS) militant group.
In addition, the neighboring countries of Turkey, Iran and Syria see that such a step would threaten their territorial integrity, as larger populations of Kurds live in those countries.


A car amplifier is one of the most important components of your audio system and needs to be matched to the speakers it will be powering. Some key considerations when choosing an amp are type, power, filters and class.
Types of Car Amplifiers
There are five main types of amplifiers available: Mono, Two-channel, Three-channel, Four-channel and Five-channel models. The type of car amp you select will depend on the number of speakers you need to power. Generally, each channel connects to one speaker. For example, mono is for one speaker, two-channel is connected to two speakers, and so on.
RMS Power Versus Peak Power
Like Subwoofer power ratings, RMS power refers to the continuous power available, whereas peak power is for brief, unsustained boosts in sound. To ensure top sound quality and avoid speaker damage, amplifiers should be matched to the speakers they will be powering.
Car Amplifier Filters
There are two types of filters: low-pass filters and high-pass filters. Low-pass filters send low-frequency tones to the Subwoofers and keep them from producing higher tones. High-pass filters send high-frequency tones to the Tweeters and prevent them from creating lower tones. By filtering the sounds, based on the speakers they are being sent to, your subwoofers and tweeters will perform better and last longer.
While there are many classes of amplifiers, there are four main ones: Class A, Class B, Class AB and Class D.
- Class A amplifiers use a single transistor, are always powered on even with no audio signal, and can become very hot.
- Class B amplifiers use two transistors and exhibit crossover distortion when switching between the two.
- Class AB amplifiers use two transistors and have a continuous, low level current running through them for improved efficiency and less distortion.
- Class D amplifiers are the most efficient, using multiple transistors and switching continuously between them while minimizing crossover distortion through a filter.


text:
History of Homťopathy
HEPARD, HILAND GEORGE, Rochester, New York, was born in West Bloomfield, Ontario county, New York, September 1, 1871, son of George Mortimer Shepard and Sarah Cornelia Crosman, his wife. He is English descent. After attending the public schools of Rochester he entered the University of Rochester, where he completed his literary education. His medical education was acquired in the New York Homťopathic Medical College and Hospital, where he graduated in 1898. During that and the next year he was interne in the Rochester Homťopathic Hospital, after which he began general practice in that city. He is a member of the dispensary staff and assistant physician to the hospital. He is a member of the Monroe County and also of the Western New York Homťopathic Medical societies. On September 23, 1902, he married Mary Houston Garrison.
Source : History of Homťopathy by William Harvey King
Copyright ę Sylvain Cazalet 2003

domain: library


“South Carolina officials requested to lift a federal court order that prohibits the State from using a racially gerrymandered congressional map for the November 2024 elections in Congressional District 1. If these officials had their way, they would conduct elections in South Carolina for the second election cycle under an unconstitutional congressional map. The Legal Defense Fund and its partners adamantly denounce this feeble attempt at proceeding under the unconstitutional status quo when time remains to hear from the Supreme Court about whether it agrees that the map is unconstitutional and then for the State to fix it.
“Rather than using the same unconstitutional congressional map that was used in the 2022 midterm elections, state officials should be working to fix an unconstitutional map of their own making and remedy the harm to voters. The Legal Defense Fund and its partners eagerly await an opinion from the Supreme Court and remain hopeful that the Court will affirm a three-judge panel’s unanimous post-trial decision that the map at issue is unconstitutional based on the strength of the case record and settled law. That affirmation is the key next step to obtaining a final fix to the unconstitutional racial gerrymandering for congressional elections in South Carolina.
“While this effort to deny voters of their right to a congressional election free from racial discrimination underpins nationwide efforts that we have fought against too, the Legal Defense Fund and its partners continue to fight attempts like these to forestall change with the determination necessary to realize the full protections of the 14th and 15th Amendments to the U.S. Constitution.”
Read our filing in opposition to the defendant’s motion for a stay here.
Founded in 1940, the Legal Defense Fund (LDF) is the nation’s first civil rights law organization. LDF’s Thurgood Marshall Institute is a multi-disciplinary and collaborative hub within LDF that launches targeted campaigns and undertakes innovative research to shape the civil rights narrative. In media attributions, please refer to us as the Legal Defense Fund or LDF. Please note that LDF has been completely separate from the National Association for the Advancement of Colored People (NAACP) since 1957—although LDF was originally founded by the NAACP and shares its commitment to equal rights.


text:
History of international trading
Scientific Disciplinary Sector (SSD)
SECS-P/12 - ECONOMIC HISTORY
I semestre dal Oct 1, 2018 al Jan 12, 2019.
The course intends to provide the fundamental elements required for an understand-ing of the international economic relationships which developed in the Modern and Contemporary Age. After an outline of the historical conditions and the motives un-derlying the expansion of Europe, the course investigates forms of organisation of production, trade and circulation of the characteristic goods from the different areas (Europe, Africa, Asia America ...) in the 15th-20th centuries with particular emphasis on the problem of the structure of interregional and intercontinental trade routes.
The course aims to provide students with the basic knowledge in the field of Com-merce History in order to acquaint historical events of the current market economy model and the comparisons among them.
European Expansion: Historical Conditions and Economic Reasons.
Production and Trade from 15th to 18th Centuries: Goods, Traffic Routes, Financial and Commercial Organization.
World Trade 1500-1650 between Old World and New World: Silky, Spice and Silver.
The Trades on the Baltic and in Southeast Asia.
World Trade 1650-1780: the Age of Mercantilism. The Rivalries between Holland, Great Britain and France. The Major Powers in Asia: India, China and Russia.
Trade and the Industrial Revolution.
World Trade 1780-1914: the Great Specialization, the First "Globalization", the Great Divergence.
The American Continent between Colonization and Independence. The USA and the Frontier.
World trade from 1914 to 1939: de-globalization.
The Great Depression.
The Collapse of the Ottoman Empire.
The Geopolitical Consequences of the World War II: Communism, the Cold War and Decolonization.
The New Layout of the International Economy (1939-1953). The Bretton Woods System and Its Transformation.
The Re-globalization: Opennes and Convergence in the Late Twentieth Century.
Unravelling the Great Specialization.
Globalization at the Dawn of the Twenty-First Century.
The Future of Globalization: Economic and Political Challenges.
R. Findlay, K. H. O'Rourke, Potere e ricchezza. Una storia economica del mondo, a cura di G. Conti, M. C. Schisani, UTET, Torino, 2017.
||Potere e ricchezza. Versione ridotta
The final exam will be an interview aiming at testing the students' overall knowledge by means of oral questions related to the required readings.

domain: economics


text: Comparative statistics of the normalized frequency distributions observed for different cellular states in master aNSC and general glioma models with and without the inputs of TCGA-LGG and TCGA-GBM RNA-Seq expression data. (A) A significant difference (Chi-Square goodness-of-fit test, P-Value < 0.001) is observed in the normalized frequency distributions while comparing the effect of TCGA-LGG expression profile in aNSC model simulation. Cellular states such as Apoptosis and NSC Renewal/Apoptosis are found to be absent in the aNSC model with TCGA-LGG expression profile. Normalized frequencies of ASPC Differentiation and GSC Renewal are also slightly increased in this scenario as compared to the master aNSC model simulation. (B) The normalized frequency distribution of the cellular states of general glioma model is well fitted with the simulation outcomes of general glioma model with transcriptomics profile of TCGA-LGG patient cohort. Similar to these analyses, mRNA expressions profile of the TCGA-GBM patient cohort is also given as inputs in (C) aNSC and (D) general glioma models. In both the scenarios, significant differences are observed between the expected and observed (outcomes from TCGA-GBM) normalized frequency distributions of the cellular states. These results prove that the developed aNSC and general glioma models are capable of differentiating the mRNA expression profiles of LGG and GBM patient cohorts correctly.

domain: biology


text:
Sophie Arnould: Actress and Wit.
Place Published: Paris
Publisher: Charles Carrington
Date Published: 1898
Description: 7 copper-plate engravings by Adolphe Lalauze; Vignette portrait on title page. 4to, original green cloth. Paris: Charles Carrington, 1898. Very good
Comments: Edition limited to 500 copies.Binding: hardcover
Condition: very good
Book ID: 36117


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text:
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Brand: Green Promise Farms
- It is fully rooted in the soil and can be planted immediately upon arrival, weather permitting. We advise not to plant during frigid winter months or under drought conditions
- For best results, plant in USDA Zones 5-8. Mature height is 18-24in , mature spread is 18-20in
- Small but impactful flowers of strawberry red petals around golden flower centers, great for a sunny border
- Grows well in full sun
- Plant will be dormant (no leaves) late Fall through the Winter months, this is normal. It will leaf out in Spring
Binding: Lawn & Patio
Details: ACHILLEA `STRAWBERRY SEDUCTION` PP18,401 Strawberry red petals surrounds a golden center although the flowers are small they prouce quite in impact clustered together atop long green stems. Strawberry Seduciton will seduce you just as easily as it will seduce the butterflies to your garden especially if planted in a sunny border. Drught tolerant once established and easy to grow, a real winner in the landscape!
Package Dimensions: 32.8 x 12.4 x 12.4 inches

domain: agronomy


text:
New York Public Library City All You Need To Know Remarkable Midtown Nice 0, New York Public Library Midtown: InteriorBeautiful interior decors. That is what amecdes brings. In addition to, the inside decors here designed beautifully using interesting and alluring ornaments and furniture. More than that, it’s ingenious display brings in amecdes from the smallest to the good spaces. For more ingenious interior designs, Verify the inside design concepts HERE.
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text:
11, Taejong-ro 65beon-gil, Yeongdo-gu, Busan, South Korea
This destination can have additional per person taxes which are excluded in the prices below
Situated within walking distance of Jagalchi Subway Station (Busan Metro Line 1), Bay Hound Hotel provides guests with a convenient base when in Busan. Guests also have access to complimentary wireles...
Distance to city centre
Pets not allowed
Policies vary by room type and provider.
+82 (0) 51 413 1114
In Busan, the average nightly rate for 2 stars hotels is 75.928. The expected nightly rate for Bay Hound Hotel is around $46; a price that is 40% cheaper than the city average. HotelsCombined considers Bay Hound Hotel to be a good deal if you’re looking to stay at a 2 stars hotel in Busan.
You can reach the front desk of Bay Hound Hotel at +82 (0) 51 413 1114.
Yes, Bay Hound Hotel has Wi-Fi available to hotel guests.
Check-out should be before 11:00 AM. For checking in at Bay Hound Hotel, guests should plan for any time after 3:00 PM on the day of their scheduled arrival.
Prices at Bay Hound Hotel will vary depending on when you plan to stay and what room type you require. The average nightly room price recently has been $46, though some users have found rates as low as $39. To see prices for your dates, enter them in the form above.
Yes, there is a restaurant on-site at Bay Hound Hotel, Busan, South Korea.
If you need to have clothes washed during your stay, you can take advantage of the laundry facilities and service at Bay Hound Hotel. Guests also have access to an iron and an ironing board during their stay - either in the room, or by asking the front desk.


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Birdland Crab Feast Apr-24
This exciting event sold out in record time! We do still have outdoor seating options for those looking to join the fun! Your table will be on our brand new outdoor deck, and you will be able to move around as you please. You will enjoy all the same perks as the original ticket purchasers!
We can’t wait to celebrate our new partnership with The Baltimore Orioles with Birdland! Join us for a very special crab feast during the team’s West Coast Showdown with The Angels. The party will also serve as a fundraiser for The Leukemia & Lymphoma Maryland Chapter!
We are thrilled to introduce the first ever Birdland Crab Feast, aimed to unite the men & women who make up one of baseball’s most passionate online fan bases! For only $65, you’ll be treated to a 3-hour party which will include all of the following in our brand new Jimmy’s LIVE Bar…
- Unlimited supply of delicious Maryland Steamed Crabs! Enjoy your favorite crustaceans while you watch The Birds!
- A commemorative “Birdland” Crab Mallet!
- A delicious and extensive buffet personally prepared by Chef Tony! We will ensure even those who aren’t trying to crack open crabs will have plenty to enjoy!
- Bottomless Beer & Wine! Enjoy over 10 choices – including Heavy Seas, Modelo, Kona Big Wave, Guinness Blonde, and more!
- Opportunities to win Orioles memorabilia, t-shirts, and more!
We also have VIP Packages available. VIP Ticket holders will receive a gift bag complete with a t-shirt, a limited-edition Guy Fieri Funko Pop, key chain, and coozie! VIPs will also receive a complimentary Patron Margarita upon arrival! Please note these packages are limited.
We’ll also have raffles and silent auctions to help raise money for LLS, one of our most beloved charities. Fans will be able to enjoy Skee-Ball, Billiards, and more during the party as well!
This event is family-friendly, but limited to only 70 tickets sold in our efforts to keep it intimate and quaint! Wear your orange, bring an appetite, and #GetFamous!

domain: entertainment