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Lung cancer is the major common cause of cancer-related mortality worldwide. In 2018, there were over 1.76 million deaths globally due to lung cancer, amounting to about 18.4% of all cancer-related deaths, and new lung cancer cases account for 11.6% of all new cancer cases (WHO, 2020). Tobacco smoking is the most significant risk factor for development of lung cancer. Compared with never-smokers, those who smoke tobacco have about 20-fold higher relative risk of developing lung cancer. Most lung cancers are known as carcinomas, and non-small cell lung cancer (NSCLC) comprises of approximately 85% of all lung cancers, with about 60% diagnosed at advanced metastatic stage when therapeutic option is no longer available (Planchard et al., 2018).
Most cancer cells, including NSCLC, exhibit diverse genetic alterations, which define as driver mutations contributing to the molecular pathogenesis of cancer. Over 60% of NSCLC harbor various oncogenic driver mutations, and these mutations in membrane receptors and downstream effector proteins lead to uncontrolled cell growth, proliferation and survival via a complex cascade of cell signaling pathways such as RAS/RAF/MEK/ERK or PI3K/AKT/mTOR pathway (Chan & Hughes, 2015). Activating mutations in epidermal growth factor receptor (EGFR), anaplastic lymphoma kinas (ALK), v-ros avian UR2 sarcoma virus oncogene homolog 1 (ROS1), Kirsten rat sarcoma viral oncogene homolog (KRAS), and v-RAF murine sarcoma viral oncogene homolog B (BRAF) have been found as oncogenic driver mutations in NSCLC (Ding et al., 2008; Govindan et al., 2012; Collisson et al., 2014). Thereafter, significant progress has been made to develop molecularly targeted agents for more personalized treatment of NSCLC patients, and several small molecule inhibitors, such as erlotinib and gefitinib targeting mutated EGFR, have been developed and used as monotherapy or in combination with chemotherapy (Schrank et al., 2018).
Metformin belongs to biguanide derivative that has been widely used as a first-line medication for type II diabetes mellitus for over 60 years and now prescribed to about 120 million diabetic patients worldwide. Metformin decreases blood glucose levels by inhibiting gluconeogenesis in liver and increasing uptake and utilization of glucose by skeletal muscle (Shaw et al., 2005). A retrospective study by Evans et al. (2005) suggested for the first time that metformin treatment is associated with the reduced risk of all cancers compared with other antidiabetic treatments in diabetic patients. Following studies showed that diabetic patients using metformin decrease lung cancer risk at the magnitude of 39%–45%, compared with those not using metformin (Hall et al., 2005; Lai et al., 2012). Several in vitro or in vivo preclinical studies also revealed diverse anticancer effects, in which metformin treatment results in a significant decrease in cell proliferation, tumor growth and colony formation, and induces apoptosis and cell cycle arrest in various human lung cancer cell lines (Ashinuma et al., 2012). In addition to monotherapy, combination of metformin with other chemotherapeutic or molecular targeted agents was shown to potentiate synergistically the antitumor effect (Morgillo et al., 2013; Tseng et al., 2013). Moreover, clinical trial also showed promising results, in which metformin treatment in combination with gemcitabine/cisplatin in non-diabetic and metastatic NSCLC patients significantly improves the objective response rate, overall survival and media progression free survival without significant increase in toxicity (Sayed et al., 2015).
The molecular mechanisms for the antitumor effect of metformin have been suggested but revealed as a much more complex nature (Vancura et al., 2018). The most well-known effect of metformin is the inhibition of complex I in the mitochondrial electron transport chain, which leads to increasing the intracellular AMP/ATP ratio. The high AMP/ATP ratio in turn phosphorylates and activates adenosine monophosphate activated protein kinase (AMPK), a heterotrimeric serine/ threonine protein kinase which regulates the multiple signaling pathways involved in cancer cell proliferation, including the suppression of PI3K/AKT/mTOR pathway (Griss et al., 2015). Metformin-mediated AMPK activation and mTOR inhibition suppress cell proliferation through reducing phosphorylation of its major downstream targets, the 70 kDa ribosomal protein kinase S6 (p70S6K) and eukaryotic initiation factor 4E-binding protein1 (4E-BP1) (Shaw et al., 2005). In contrast to metformin-induced inhibition of PI3K/AKT/mTOR pathway, there is contradictory effect of metformin on RAS/RAF/MEK/ERK pathway in NSCLC cells. Several studies showed that metformin inhibited ERK activation (Do et al., 2013; Ko et al., 2019), while activation of ERK in response to metformin was also reported (Morgillo et al., 2013). Considering the presence of compensatory loops that activate one pathway following the blockade of the other signaling cascade especially in cancer cells with RAS mutation (De Luca et al., 2012), the activation of ERK could result from inhibition of PI3K/AKT/mTOR pathway in response to metformin treatment, requiring blockade of both pathways for more efficient antitumor effect.
The present study, therefore, undertook to determine the combined effect of metformin and trametinib, a MEK inhibitor, on cell viability in NSCLC cell line NCI-H2087 with coexistent mutations of BRAF and NRAS. Here, we show that metformin induces the activation of ERK, and the combination of metformin and trametinib gives synergistic effect on cell survival in treatment with low doses, and antagonistic effect when treated two drugs with high doses.
MATERIALS AND METHODS
The human NSCLC cell line NCI-H2087 was purchased from Korean Cell Line Bank (Seoul, Korea). The cells were cultured in RPMI 1640 (Sigma-Aldrich, Gillingham, UK) supplemented with 10% (vol/vol) heat inactivated fetal bovine serum (Gibco BRL, Grand Island, NY, USA) and 1% streptomycin/penicillin at 37°C in a humidified atmosphere consisting of 5% CO2 and 95% air. Cells were maintained mycoplasma free by treating 5 μg/mL of Plasmocin (InvivoGen, California, CA, USA). Trametinib was obtained from LC Laboratories. The compound was initially dissolved in dimethyl sulfoxide (DMSO, Sigma-Aldrich) to a concentration of 1 mM and further diluted in RPMI 1640 media. Metformin (also known as 1,1-dimethylbiguanide hydrochloride) was purchased from Sigma-Aldrich and dissolved in RPMI 1640 media to a working concentration of 100 mM.
MTT assay was applied to measure cell viability as described previously (Kim et al., 2018). Briefly, cells were harvested and seeded in 24-well plates at a concentration of 5×104 cells/well for 24 h. Then, cells were treated with increasing concentrations of trametinib (2.5–40 nM), metformin (0.25–4 mM), their combinations or vehicle control for 72 h. Experiments were performed in triplicate, each conducted in quadruplicate. The IC50 values (concentrations of drugs resulting in 50% decrease in cell viability relative to controls), combination index (CI) and drug reduction index (DRI) were calculated using CompuSyn software (ComboSyn). The CI value is a quantitative measure of the degree of drugs interaction. According to the recommendation of Chou-Talalay (Chou & Talalay, 1981), CI<1 indicates synergistic effects of drugs; CI=1 indicates additive effect; CI>1 indicates antagonism. DRI denotes how many folds of dose reduction are allowed for each drug due to synergism as compared to the dose of each drug alone.
Western blotting assays were carried out as previously described (Kim et al., 2018). Primary antibodies included pERK1/2 (Tyr204), ERK1/2, cyclin D1, β-actin (all from Santa Cruz Biotechnology, Dallas, TX, USA), and p4E-BP1 (Ser65), 4E-BP1, pS6 (Ser240/244), pS6 (Ser235/236), S6, pAMPKα (Tyr172), AMPKα1/2 (all from Cell Signaling). Following incubation with secondary antibodies conjugated to horseradish peroxidase (Cell Signaling), immunoreactivity was detected with enhanced chemiluminescence method (Santa Cruz Biotechnology).
Cells were plated in 6-well culture dishes at a density of 600 cells per well. After 24 h, cells were treated with metformin, trametinib and their combination. Every three days, medium was changed with fresh medium containing the corresponding concentration of the drugs. Following 15-day treatment, cell colonies were washed with cold PBS and then fixed with ice-cold 100% methanol. Cells were stained with 0.1% crystal violet in 20% methanol for 10 min and pictures were taken with a digital camera (Olympus).
To delineate whether single agent metformin and trametinib could affect the activity of cell signaling pathways in NCI-H2087 NSCLC cells, we first analyzed the alterations of main downstream effector proteins of PI3K/AKT/mTOR and RAS/RAF/MEK/ERK pathways following treatment of metformin and trametinib for 24 h. As shown in Fig. 1, metformin suppressed the levels of p4E-BP1, pS6 (Ser235/236), pS6 (Ser240/244) in a dose-dependent manner, and increased the phosphorylation of AMPK. Interestingly, metformin increased the levels of pERK especially at high doses of 2 mM and 4 mM. On the other hand, trametinib inhibited the levels of pERK, pS6 (Ser235/236), but did not significantly affect the activity of AMPK, 4E-BP1 and pS6 (Ser240/244). These findings suggest that metformin exerts its effect in the direction of inhibiting PI3K/AKT/mTOR pathway, but simultaneously plays its effect toward activation of RAS/RAF/MEK/ERK pathway in NCI-H2087 NSCLC cells.
Next, we asked what could be happened in cell survival when combined metformin with trametinib. To this end, NCI-H2087 cells were exposed to varying concentrations of drugs as single agent or their combination for 72 h, and cell survival analysis was performed using MTT assay. As expected, relative cell viability was decreased following treatment of metformin and trametinib in a dose-dependent manner. IC50 values (concentrations of drugs leading to 50% decrease in cell viability relative to controls) for metformin and trametinib were 3.2 mM and 108.4 nM, respectively. Combination of two drugs at low doses resulted in a greater inhibition of cell viability than those of metformin or trametinib alone, while the combination treatment at high doses did not show the additively increased inhibition of cell viability (Fig. 2A). To quantify the response of NSCLC cells to the combination of metformin and trametinib, we combined two drugs in a constant ratio to each other and measured CI and DRI using CompuSyn software. The CI values in combination at low doses ranged from 0.62 (at the combination of 0.25 mM metformin and 2.5 nM trametinib) to 0.84 (at the combination of 1 mM metformin and 10 nM trametinib) indicating synergism according to the method of Chou-Talalay (Chou & Talalay, 1981). On the other hand, CI values in combination at high doses were 1.33 (at 2 mM metformin and 20 nM trametinib) and 2.08 (at 4 mM metformin and 40 nM trametinib) denoting antagonistic effect between two drugs (Fig. 2B). The DRI values displayed a similar pattern to that of CI values; combination of two drugs at low doses showed a remarkable drug reduction effect with DRI Values above 1, and at high doses little drug reduction effect with DRI values of metformin below 1 (Fig. 2C).
To evaluate the mechanisms underlying the synergistic growth inhibitory effect between metformin and trametinib at low doses, we characterized the effect of this drug combination on downstream regulatory proteins of cell signaling pathways involved in cell survival and proliferation using Western blot analysis. After 24 h of treatment, the combination of metformin and trametinib led to the enhanced suppression of pERK, p4E-BP1, pS6 (s240/244), pS6 (s235/236) compared to that of single agent treatment, and trametinib revealed little effect on metformin-induced activation of AMPK (Fig. 2D).
In the present study, we showed that the combination of metformin and trametinib in treatment with low doses synergistically inhibits NSCLC cancer cells. To extend these results into long-term effect of the combination treatment, we tried colony formation assay by culturing NCI-H2087 NSCLC cells for 15 days. As shown in Fig. 3, treatment with metformin and trametinib alone resulted in a partial inhibition of colony formation, whereas the combination treatment with low doses enhanced the inhibitory effect on the formation and growth of cell colonies as compared with either agent alone. These results further support the synergistic growth inhibitory effect of metformin and trametinib combination on cell viability assayed by using MTT assay.
Conventional clinical therapeutics for NSCLC depends on surgical resection of tumor mass, radiation therapy and chemotherapy. Chemotherapeutic strategies, such as cisplatin monotherapy or platinum-based combination therapy, give limited benefits due to drug resistance and serious side effects resulting from killing of normal cells (Stinchcombe et al., 2010). In NSCLC, diverse molecular alterations in oncogenic genes involved in cell survival and proliferation have been known, such as EGFR, RAS, PI3k, and BRAF. Recently, several small molecule inhibitors targeting these oncogenic mutations have been developed and proved their efficacies in the treatment of NSCLC. Although these targeted agents achieved remarkable progress for NSCLC patients with better overall response rate and progression-free survival compared with chemotherapeutic agents, most patients eventually experience relapse due to acquired drug resistance to these agents (Liu et al., 2020). Therefore, the new therapeutic strategies to overcome the acquired resistance is required in the field of NSCLC oncology and current research is focusing on multidrug combination therapy with minimal side effects.
The antidiabetic drug metformin proved to be well-tolerable drug with safety profile and low cost. Moreover, there is no report for acquired resistance to metformin although it has been used as first-line treatment for type II diabetes over 60 years. Metformin as monotherapy or combination therapy inhibits the growth and proliferation of NSCLC cell in vitro and in xenograft model (Yousef & Tsiani, 2017). Our previous studies also suggested that metformin inhibits mTOR activity via activation of AMPK and suppresses the phosphorylation of mTOR substrates, 4E-BP1 and S6 in melanoma and colorectal cancer cells. We also showed that metformin inhibits the activation of ERK, a key mediator of RAS/RAF/MEK/ERK proliferative signaling pathway (Kim et al., 2018; Ko et al., 2019).
However, the effect of metformin on RAS/RAF/MEK/ERK pathway has shown some controversial results in NSCLC cells. Several studies suggested that metformin treatment inhibits ERK phosphorylation in diverse NSCLC cells (Do et al., 2013; Ko et al., 2013). Morgillo and his collaborators (2013), on the other hand, showed that exposure of metformin to NSCLC cells leads to phosphorylation and activation of ERK through an increased BRAF/CRAF heterodimerization. Our present study also showed a remarkable increase of ERK phosphorylation and suppression of mTOR signaling pathway following metformin treatment in NCI-H2087 NSCLC cells. Although crosstalk and compensation effect between signaling pathways have been well recognized (De Luca et al., 2012), the molecular mechanism or cellular context underlying metformin-induced ERK activation is unclear. Since BRAF/CRAF heterodimerization is a key event for the activation of ERK specifically in RAS mutant cells (Holderfield et al., 2014), metformin-induced activation of ERK is most probable in cells with activated RAS. However, activation of ERK after metformin exposure is observed in NSCLC cells with wild-type RAS (Corte et al., 2016). In addition, Martin and his colleagues (2012), using melanoma cell with BRAF mutation, demonstrated that metformin-induced AMPK activation targets and reduce the DUSP6 protein, a phosphatase acting as ERK-negative regulator, which results in increased ERK activity and acceleration of cell growth.
The metformin-induced activation of ERK could be important in cancer therapeutics, since metformin, via activation of ERK, could compromise its well-known anticancer activity suppressing mTOR signaling pathway. Therefore, we reasoned that suppression or activation of ERK activity in response to metformin treatment could be the predictive biomarker for synergism or antagonism when combined metformin with other molecular targeted agents. To elucidate this subject, we combined metformin with trametinib, a MEK inhibitor, and found that the combination synergistically decreases cell viability in treatment with low doses of two drugs, while it gives antagonistic effect at high doses. Our result is inconsistent with others (Corte et al., 2016) in which the combination of metformin with MEK inhibitor selumetinib induces synergistic anti-proliferative effect in NSCLC cell lines, independently from the RAS mutational status. In the present time, we have no idea for this difference, but we speculate that in our cell model metformin-induced activation of ERK in combination of two drugs at low doses is not strong enough to overcome the trametinib-induced inhibition of ERK activation, but at high doses metformin counteracts the trametinib-induced inhibition of ERK activity, which in turn leads to cell proliferation and antagonistic effect. Another plausible explanation for the discrepancy may be the difference of mutational status in NSCLC cells between the studies; whereas we used NCI-H2087 cells with BRAF mutation, Corte and his colleagues employed NSCLC cells with wild type BRAF. In melanoma cells with mutant BRAF, metformin-induced increase of pERK is dependent on AMPK-induced degradation of DUSP6 protein (Martin et al., 2012). In NCI-H2087 cells with coexistent mutations of NRAS and BRAF, therefore, AMPK-mediated DUSP6 protein degradation could potentiate the pERK increase induced by BRAF-CRAF heterodimerization occurred in the context of RAS mutated cells. In BRAF wild-type cells, in contrast, trametinib may counteract pERK increase by metformin-mediated BRAF-CRAF heterodimerization, since MEK inhibitor acts downstream of RAS/RAF/MEK/ERK signaling pathway.
To our knowledge, this study is the first report to show biphasic antitumor effect in the combination therapy of metformin and trametinib using NSCLC cells. These findings suggest that the efficacy of metformin and trametinib combination therapy may depend on the alteration of ERK activity induced by metformin and specific cellular context of cancer cells such as mutational status. Further studies are required to elucidate the effect of metformin and trametinib combination in treatment with high doses, and in vivo effect of this combination therapy.


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his is a rare 2 disc show where both discs featured great wrestling. The 6 man matches were exciting. The Morishima vs Kanemaru match featured a believable clash in style and tactics. The surprise of Disc 1 was Suzuki vs M. Yone. In most scenarios, Yone is good for taking his lumps in a tag match but, not much else. Here he was allowed to revert to BattlArts Yone against the legend Minoru Suzuki...which was great stuff. Very stiff and grapple oriented... I highly recommend it.
Disc 2 featured the main events. KENTA vs SUWA lived up to expectations but, I didn't think it was a MOTY contender. It was a great match with a different feel (much like Kobashi vs Ogawa '03) but didn't kick into overdrive at the end like I would have liked. Still the match & finish are great just not an all-time classic.
Kobashi & Take vs Akiyama & Tenryu was the dream match I was looking for. Again, its not an all time classic but, a great match where 2000s Take brings his Holy Demon Army boots out.
The GHC title match is highly recommended as well. I'm a bigger Rikio fan than most. He certainly had the toughness and size to be a champ. A pretty good outing for Misawa here as well. A nice closer to a great show.
All in all a great set to get for fans of 2000s NOAH.Rating: [5 of 5 Stars!]

domain: hobby


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Wednesday, November 25, 2009
Shout Out Your Thanks
I am thankful for shooting stars. I saw one last night and shouted, "Thanks, God, that was awesome." Then I laughed with delight.
What would you like to shout in thanks to God? Do it, then laugh in delight for having done it.
Posted by Lisa Gonzales-Barnes


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❶If you have the choice, and you are on a slow connection, Word documents will download faster than Adobe documents.
Dundee ms sex
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domain: geography


Please send a list of Welding Procedures
- Posted by pete
- On March 9, 2023
- 0 Comments
- weld procedures
We recently received a request from a client for a list of Welding Procedures (WPS) to suit pipe welding.
While that may sound like an obvious and simple request, the reality is that a WPS it’s just not as simple as a list of instructions on how to weld pipes together.
It’s just not that simple.
While there are probably common welding projects that a simple Welding Procedure might be adequate for, the WPS that Australian Welding Solutions produces are for specific applications and are individually crafted for that project and the related engineering, quality and safety standards.
This is especially true for most pipe welding projects, where a range of technical factors and specifications are factored into the WPS preparation.
Our Welding specialists will consider all of the relevant technical information when designing a WPS to ensure a compliant weld will be applied.
What is a Weld Procedure?
A Welding Procedure is a document that outlines the specific steps and requirements for carrying out a welding operation. It includes detailed instructions on the type of equipment to be used, the welding techniques to be applied, and the safety measures that should be taken.
A welding procedure aims to ensure that a consistent and reliable weld is produced every time the procedure is followed. It also ensures that the welding operation meets the required standards and specifications for the particular application.
Welding procedures are developed by qualified personnel based on the type of metal being welded, the welding process being used, and the project’s requirements and related standards. Once a welding procedure has been developed, it must be qualified through a series of tests to ensure that it meets the required standards and specifications.
Welder qualification to a WPS
Generally not well understood is that welders are also required to be ‘qualified’ to undertake the welding specified by a WPS.
This may involve training and assessment of a welder’s ability to deliver the weld to the level specified by a WPS.
If the welder qualification is not completed, then the weldment does not comply to the WPS, and therefore does not comply with the related standards.
This is a key point that may result in the weld not complying with the design standard which in turn, may compromise the entire project.
Why there is no place for a ‘list of WPS’
So, unfortunately, we can’t simply provide a list of WPS to be arbitrarily selected for a job, and then welded by a random welder, even if they are a competent welder with a history of quality weld production.
A structured process is performed by highly qualified and experienced personnel to provide the WPS, and then a training/certification process to ensure the welder can deliver welds consistently to the WPS standards. AWS also provides Welder Qualification to the WPS.
Failure to deliver on these WPS requirements may compromise the weld integrity; risk legislated penalties and also risk lives.
Federal and State legislation now enforces liability, prosecution and substantial fines on persons involved in the manufacturing process, including the organisation’s management. Consequently, WPS preparation and welder certification to a WPS are not trivial matters.


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With the ACCC recently publishing its draft guidance to improve the integrity of environmental and sustainability claims made by businesses and protect consumers from ‘greenwashing’, it’s time for businesses to take their commitment to climate change seriously.
The guidance was released off the back of the ACCC’s alarming finding that 57 per cent of businesses it reviewed were making potentially misleading environmental claims.
In releasing the report, the ACCC’s Chair Gina Cass-Gottlieb said businesses needed to be honest and transparent when making environmental or sustainability claims so consumers are not being misled.
She pointed out that false or misleading claims can undermine consumer trust across the board and put businesses that are taking genuine steps to adopt sustainable practices at a competitive disadvantage.
At ICN, we believe that most businesses are trying to do their best. They clearly understand the need to take climate action to remain competitive in a changing marketplace driven by climate targets. But many are confused about how to start, what to measure, how to ensure their data and calculations are accurate and what steps they should take to reduce. They are also concerned about the cost to the business, especially during an economic downturn.
That’s why ICN has joined forces with ClimateClever, a small, 100% Australian-owned climate-tech company that helps SMEs to get started on their climate journey. Its mission is to drive meaningful and measurable impact on climate change by making climate action affordable and accessible for everyone.
The ClimateClever platform gives small and medium businesses an efficient, cost-effective way to accurately measure their carbon footprint throughout the year. It can also help you identify strategies to reduce, as well as offset, your emissions to become carbon neutral.
Also, if you sign up to ClimateClever through ICN you receive a 10% discount on a paid tier.
Find out how you can start your climate and sustainability journey here.
We also encourage businesses to give the ACCC feedback on the draft guidance.

domain: news


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Monday, September 28, 2009
9 is really just a 6
Shane Acker's 9
This last Saturday I got to see 9, the new animated, sock-puppet, post-apocalyptic action fantasy adventure. 9 was one of those movies I've had on my radar for quite some time, so I'm glad I got to finally go. As you can tell from my title though, it was a tad lacking.
I'm not going to dwell too much on 9, it was a pretty simple movie, and I think a pretty simple review will suffice. Visually, this movie is as beautiful as you may have guessed from just a simple viewing of the trailer. It was simply stunning, the movement of the characters on screen, the scope of the blasted, ruined landscape, and the plethora of minions, all creepy-crawley in their mechanical design were marvelous. The attention to detail was great, and the setting was an alternate history similar to the 1930's, but with the twist of "dark science" thrown into the mix, then blasted by horrible war between man and machines. Isn't that always the case though, you can't trust friggin' machines! The animation was pretty much as good as it gets, and didn't strive to be believable, just fantastic. It was visually beautiful.
But, that's about it. The story was flat, predictable, and simple. The characters, these nine sock puppets, were monotonous and two dimensional in their personality, which was a function of the not-so-complex plot. Because of this though, you really developed nothing, absolutely nothing for any of them. If a crazy, skeleton-machine hybrid chomps one of them in half, so what, there's eight more. Plus there's a convoluted thread of a story unraveling all over the place, no pun intended. You'll be scratching your head, 'til they throw you a bone in the form of a half-baked solution...that you really shouldn't think about too hard.
Maybe I'm too picky, I know many went to 9 and came out in love with it, based mostly on the visuals, sure, but you really can't give too much weight to the story. It's enjoyable, but not deep, and it's incredibly short at just 79 minutes, so it doesn't even really fill you up all that much when it's all said and done. I liked the movie, how it looked and the fantastic world it presented, but I won't be rushing out to see it a second time. I liken it to going out to your favorite Mexican restaurant, intent on a wonderful meal, but only eating the free chips and salsa that come to your table and then leaving; you got to see all the good stuff other people were eating, you got to sit down for friendly service, you got to smell the wonderful food and hear the music full of ambiance, but you left empty and hollow. That's 9.
"Gasp! What, no plot, story?!?!?!"

domain: artistic


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Regardless of political opposition to sex education, about 68% of boys and girls in schools want adolescence education, revealed a study to be released today. The students however wanted sex education from someone other than their class teacher, according to the study conducted by the Swedish Association for Sexuality Education. The study was carried out in Haryana, Uttar Pradesh and in a few slums of Bangalore. Based on sex education imparted in schools in the last five years, it found that students were not comfortable asking their class-teachers sensitive questions about sex. In schools where adolescence education was delivered, about 91% students in urban areas and 95 per cent in rural areas said the decision to have a baby should be made by both partners in a marriage.
A significant number of girls who had been through the programme said they would decline to have sex without a condom and oppose sexual abuse. It was also found that the number of girls are coming forward to obtain knowledge about sexually transmitted diseases increased from 27.9% in 2003 to 42.3 % in 2008. Class 10 students, the survey said, who had been through the curriculum, were able to reject common misconceptions about masturbation and HIV. But class 11 students, who had not studied sex education failed to do so. Girls in class 10 were able to understand that the contraceptive pill did not protect them from sexually transmitted diseases and HIV.


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For well over a decade, the ‘Real Housewives of Atlanta’ has dominated as one of the standard-bearers in the reality TV realm – blending drama, empowerment, and entertainment to potent effect.
And, as most viewers of the hit series will attest, Kenya Moore has long served as a nucleus of the show.
Indeed, whether it’s twirling on her foes, building a business empire, or sharing her relationship and motherhood journeys, there’s no denying that the Miss USA winner wears one of the shiniest crowns across the entire Housewives franchise.
In celebration of season 14 of the RHOA and the icon’s visit to London, That Grape Juice teamed up with Hayu to host An Evening with Kenya Moore.
The intimate event was housed at Saint Aymes – the beautiful, boutique London space owned and operated by entrepreneurial sisters Lois & Michela.
Hosted by Wunmi Bello, the occasion saw guests treated to candid conversation with Kenya – who opened up in earnest about her journey on the show and her life’s journey more broadly.
Unfiltered, unbridled, and unrestricted, the star shone a light on many corners of her story and shared many words of encouragement.
With Ms. Moore in the building, fun times were an assurance. Guests drank celebrity favorite bubbly Luc Belaire, as well as a colorful collection of cocktails courtesy of Clije, Mattuga Distillers, Bumbu, Liv Rums, and The Parlor. Delicious TGJ cakes and cookies were provided by Yours Truly Ola.
All in attendance were gifted goodie bags that included ‘Grown: The Black Girls’ Guide to Glowing Up’ (courtesy of Bloomsbury Books), as well as sought-after must-haves from Cantu, Ruka, Waken, Fud, Dibia, As Raw As, Adesha Beauty, and Hayu.
Head below for more from an incredible night…
Lit Libations & More
Goodie Bags for All
New episodes of the ‘Real Housewives of Atlanta’ season 14 are available to watch every Monday on Hayu.
Check out our exclusive playlist for An Evening with Kenya Moore
They date some ugly men . Kenya ex husband was ok.. she doesn’t date ugly men cause ewww
Kandi and Todd is an attractive couple. Todd not bad.
Yas Miss Ken!!! Give us that BDE 😍

domain: celebrity


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Doc traces meteoric career of architect Eero Saarinen
PBS presents ‘Eero Saarinen: The Architect Who Saw the Future’ on Tuesday, which traces the Detroiter’s remarkable career
Eero Saarinen, son of the man who built Cranbrook, was a modernist architectural giant just hitting his stride when he was felled by a rapidly advancing brain tumor in 1961.
He died on the operating table at the University of Michigan Hospital. He was just 51.
Saarinen was the creative genius behind the Gateway Arch in St. Louis, the Jetsons-style TWA Terminal at Kennedy Airport, the General Motors Technical Center in Warren, Dulles Airport outside Washington, D.C., and much of the furniture at Kingswood School in Bloomfield Hills.
On Tuesday, PBS “American Masters” will present “Eero Saarinen: The Architect Who Saw the Future,” which traces the meteoric trajectory of this remarkable career.
Giving the story added punch is the fact that it’s narrated by Eric Saarinen, Eero’s son from his first marriage to sculptor Lilian Swann Saarinen. When Eero married his second wife, New York Times art critic Aline Bernstein Louchheim, he largely abandoned his original family.
What could have been just a straight profile of a significant architect morphs into a deeper story about a son’s quest to know and reconcile with his late father,
“I hated my father,” Eric said by phone from his home south of Los Angeles. He was just 12 when his father left his mother.
“We were kicked out of the house,” he said. “That was an awful thing. My father kept the house, so all our friends, and the school right next door — that was all gone.”
Eric, his sister and mother moved to Massachusetts.
Eric, who’s spent decades filming TV commercials, was initially reluctant to sign on when first approached by filmmaker Peter Rosen, who’s produced “American Masters” episodes on Jascha Haifetz, Garrison Keillor and Arthur Rubinstein.
Finally Eric gave in, on the condition that he act as director of photography, as well as narrator — instinctively recognizing that photographing his father’s work, which he didn’t know well, might clear a path to understanding the man whose memory had been, up ’till then, mostly bitter.
“So I had to study up,” he said. “I had a pile of books probably three feet high of all my father’s work, because I really wanted this to be a true story.”
The three-year project, Eric said, ended up being “very cathartic and psychologically centering for me. My whole goal,” he added, “was to raise my dad out of the ashes.”
History has not necessarily been kind to his father, though he’s come in for more-sympathetic appraisal in recent years.
Eero, whose practice was in Bloomfield Hills, was always an outlier in the modernist movement. His chief sin was to produce some buildings, like the 1962 TWA Terminal, that were downright sculptural in an era that deplored any deviation from the rectilinear norm.
“Saarinen was not afraid to be expressive,” said Reed Kroloff, former director of the Cranbrook Academy of Art. “And that was heresy. It became less so in the later ’60s,” but he was dead by then.”
Critics complained that Saarinen’s buildings all looked different, that they didn’t advance a consistent design philosophy.
And indeed, the rectilinear elegance of Saarinen’s John Deere headquarters in Moline, Illinois, for example, has little in common with the swooping curves of the TWA Terminal.
Yale University architectural historian Vincent Scully sniffed that Saarinen’s buildings were nothing more than corporate branding — architectural billboards, if you will.
“Like most critics at the time,” Scully told The Detroit News in 2007, “I didn’t like his work.”
But over the years he had a change of heart.
“The thing I feel worst about is TWA,” Scully said. “I didn’t fly out of it until after Saarinen’s death, and it’s really remarkable.”
One of the pleasures for Metro Detroiters in the PBS special will be seeing the Cranbrook Educational Community, where Eero grew up and first started designing with his father, Eliel, filmed by a master.
“When Eric decided to work with Peter Rosen,” said Gregory Wittkopp, director of the Cranbrook Archives, “he knew the story had to be grounded at Cranbrook.”
The project in many ways sprang from local sources. One of the prime motive forces was Bloomfield Hills architect Robert Ziegelman, who trained in Saarinen’s office when he was young.
For his part, Wittkopp, who’s an architect, places Eero Saarinen in the top ranks of 20th-century architects.
“I like to quote Minoru Yamasaki,” he said, alluding to the Detroit architect who designed the World Trade Center towers in New York. “He often said Eero was a sculptor about to become an architect.”
8 p.m. Tuesday
Detroit Public TV — Channel 56
Significant Eero Saarinen buildings
1956: General Motors Technical Center, Warren
1960: U.S. Embassy, London
1962: TWA Terminal, Kennedy Airport, New York
1963: Dulles Airport, outside Washington, D.C.
1964: John Deere World Headquarters, Moline, Ill.
1965: Gateway Arch, St. Louis


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DIFFERENT real estate companies have different ways of counting vacant square footage, but they agree on one thing: there are a lot of very big blocks of commercial office space available in New Jersey right now. Some real estate executives suggest it may an unprecedented amount; others simply point out that the situation compares with the state of affairs during an overbuilding crisis in the late 1980's and early 1990's.
''The development community is probably healthier than it was back then,'' said Tom Stanton of the Staubach Company, which is marketing 520,000 square feet at the Morris Corporate Center IV in Parsippany for sublease from Aventis Pharmaceuticals. ''But there is such a lot of sublease space available that the group of buildings over 250,000 square feet is unusually large.''
Using the common practice of counting contiguous large blocks as one, market analysts at CB Richard Ellis and Insignia/ESG each report more than a dozen blocks larger than 250,000 square feet are available to lease or sublease -- totaling more than 5 million square feet. In 1999 and 2000, when the commercial market was hot, only five such blocks were ever available at one time, according to CB Richard Ellis.
A wave of corporate consolidations is the most important reason for the current situation, commented Insignia/ESG's executive director in New Jersey, Patrick Murphy. ''Companies keep consolidating and merging and moving out of various medium-to-large offices into a single big headquarters,'' he said. ''That makes for both a lot of sublease space available and fewer companies who are in the market for big spaces.''
Thus, brokers are being forced to focus increasingly on ''courting a few 2,000-pound gorillas,'' as one put it, and on demonstrating why a client's property would suit a particular behemoth best.
''Now is the time when strategy becomes critical,'' said Geoffrey L. Schubert, a commercial broker with CB Richard Ellis who is part of a team marketing the newest big block available. Last month, Mr. Schubert began marketing 528,000 square feet in two contiguous buildings at Southgate Corporate Office Center in Morris Township.
Southgate arrives as the second-biggest block on the market, smaller only than offices at Somerset Grove I and II in Franklin Township. That 860,000-square-foot block consists of a 446,700-square-foot office formerly occupied by AT&T, on the market for 11 months, and a contiguous 413,000-square-foot office owned by Merrill Lynch, which consolidated in Princeton seven months ago.
The Southgate space, being offered for lease as either one or two offices, was occupied by two technology companies for nearly 20 years through the companies' various mergers and corporate configurations. Telcordia filled 320,000 square feet in one building, and Lucent Technologies inhabited 208,000 square feet in the other. Mr. Schubert and his partners began some months ago to seek a sublessor for the last two years of Telcordia's lease. After Lucent vacated its office, they began to market the entire block at Southgate for direct lease from the building owners.
''This building has been upgraded several times,'' Mr. Schubert said. ''It's a nice campus with amenities, a cafeteria, large parking space, a big county park across the street, a great environment to accommodate up to 2,000 people.
''It is a little off the beaten track, on the other hand, about half mile from downtown Morristown. It is older space. It's vacant. There is a great deal to be had for some tenant, and the owners are ready to do it.''
Mr. Schubert said his strategy would entail homing in on businesses in two types of situations: those who may be downsizing or just looking to cut down on the cost of office space and those looking for inexpensive-yet-functional ''disaster recovery'' space in the event of a terrorist attack or calamity.
Mr. Schubert said he was aware of a few companies looking for disaster-scenario space in the wake of continuing terrorist threats and of plenty of businesses looking to trim their budgets. He cited the purchase last year of the old American Cyanamid office building in Wayne by Toys ''R'' Us as an example of the latter. ''They occupied good buildings in multiple locations and went out and bought cheap, did the rehab and wound up paying less for space under one roof,'' Mr. Schubert said. ''Those are the kind of tenants we can find for Southgate.''
He is trumpeting the Morris Township office as a cut-rate bargain located in the general neighborhood of office space priced $30 to $35 a square foot. He said, ''We could get the deal down to below $25 per square foot gross for the whole half-million square feet on a 10-year lease.'' He added, ''That could be $8 or $9 a square foot net savings over the term of the lease, which could go directly to a company's bottom line.''
In general, Mr. Schubert said, the smartest strategy he sees for brokers of large space is: ''Think like a property owner. Know the asset backward and forward, so you can always come up with reasons why your building is better than the next. Also, think like a tenant. If a company's employees are used to being in an A building, then I need to figure out how to make my building work for them. Maybe put in a new athletic facility? Improve the cafeteria?''


Border Wall Funding
Written by : Ashleen Nowotny
Illegal immigration has been a big issue for some time now, but yet the democrats proceed to ignore the actually issues that come with it. A partial government shutdown was forced due to the democratic leaders not wanting to fund the wall. Rep. Matt Gaetz made a good point but asking if the Democrats are negotiating for the American people, or the Illegal Immigrants. We need the wall more now than ever. The amount of illegal immigrants in this country is absurd. President Donald Trump has made it clear to America that he wants to start off the new year with something big, and the wall is the perfect opportunity to do so. Funding for the wall is a great step towards it.
Thursday night Vice President Mike Pence had an interview with Tucker Carlson. Pence confirmed that the White House would reject any Democratic spending bill without funding for a border wall. Mike Pence called in “no deal” when the Democrats won't accept the idea. To accept the idea would only be sensible.
Now that the democrats have control of the House, they can pass bills ending the partial government shutdown. But with the GOP Senate those bills are going nowhere. The Democrats will do everything in there power to shut this down, but there is no chance in the world that they will be able to make Donald Trump stop his plans. There is no excuse for what illegal immigrants are doing, so why let it go on. Just like Charlie Kirk said, this is a national security issue. We currently have 44 thousand illegal immigrants in our federal prisons. An illegal immigrant is twice as likely to commit a crime than an American Citizen. Tens of thousands of kids are being sex trafficked across the US/Mexican border, and 98 percent of all the heroin in America comes across the border. The cost of illegal immigration in America is 115 BILLION dollars a year. If we ever needed a change, its now.


Department Chair for Communications and Journalism
Associate Professor of Communications
Vicki Welch has taught communication courses at Dakota Wesleyan for more than 20 years. She is the department chair of communication and of journalism, and has taught courses covering all aspects of both disciplines.
Her degrees are from South Dakota State University, where she worked with SDSU Forensics and with Prairie Repertory Theatre.
Welch’s teaching philosophy centers on assisting students to achieve their full potential, especially in discovering their strengths as researchers, organizers and public speakers. “We all have important ideas to share. Competent communication is how we succeed in the world.”


Flying Lotus unveiled a cosmic mini-suite of two new songs, “Spontaneous” and “Takashi,” from his upcoming album, Flamagra, out May 24th via Warp.
“Spontaneous” features Little Dragon vocalist Yukimi Nagano singing over a roiling low-end groove shot through with dusty drums and and a galaxy of various synth sounds. After just a couple minutes, “Spontaneous” fades out and gives way to the opening thump of “Takashi.” The song — a dense, dizzying jam centered around a skittering clavichord — is named for Takashi Kudo, an artist in the teamLab collective, whose worked helped re-inspire Flying Lotus’ own creative side.
“Spontaneous” and “Takashi” follow previously released Flamagra track, “Fire is Coming,” which featured David Lynch. The album will also boast contributions from Anderson .Paak, Solange, George Clinton, Tierra Whack, Denzel Curry, Thundercat, Shabazz Palaces and Toro y Moi.
Flamagra marks Flying Lotus’ first studio album since 2014’s You’re Dead! In the years since, the producer/DJ has released scattered singles and worked as a producer on projects like Kendrick Lamar’s To Pimp a Butterfly and Thundercat’s Drunk. In 2017, Flying Lotus released his feature film debut, Kuso.


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At least one member of 34 per cent of households lost jobs or earning opportunities across the country in the last year due to the Covid-19 pandemic, according to a survey.
Besides, 77.23 per cent of the households surveyed said their monthly average income decreased comparing to the pre-pandemic period, it revealed.
The extent of job loss and income decrease was also higher in households with migrants comparing to others, said the survey titled 'Demographic and socio-economic changes induced by the Covid-19 pandemic in Bangladesh: Dynamics and challenges of new circumstances.'
The findings of the survey were presented at a virtual dissemination programme on Wednesday evening.
Non-governmental organisation BRAC, UN Women and New York University had jointly conducted the research which highlighted the demographic, economic and social changes in Bangladesh's urban, per-urban and rural areas induced by the reverse migration.
BRAC's Senior Director KAM Morshed presented the key findings while New York University's centre on international cooperation programme lead Leah Zamore moderated the discussion.
Country Representative of UN Women Bangladesh Shoko Ishikawa, Columbia University acting director Daniel Naujoks and a director of BRAC USA Adam Schwartz also spoke at the virtual programme.
According to the research, most of the households received inadequate public support that is helpful to reduce the debt burden.
"A higher percentage of urban household or 24 per cent reported receiving support compared to rural ones (15 per cent)," it said.
As a result, the family savings and net worth depleted substantially, the report said, adding that savings fell by 62.31 per cent and household debt increased by 30.9 per cent.
The research data was collected from 6,370 households in 21 districts, focusing on migration-prone regions between December 10 and December 25 with the reference period of April-October 2020.
The research also revealed that international returnee migrants aged 35 years and above were 44 per cent, many of whom had a lesser chance to go back due to age-related risk aversion and other factors.
The research recommended that policymakers should take initiatives to allocate additional resources for the support of dependent people including old aged at the local level. It also said the strong coordination between the government and private sector is needed to facilitate the reintegration of returnee migrants.
"Also the smooth return of international migrants to their workplace should be facilitated with the help of destination countries and UN agencies," it said.
Referring to the research, Shoko Ishikawa said the long closure of schools in Bangladesh due to the pandemic culminated into some social challenges including child marriages.
She also underscored the need for providing the women migrants with special support so that they can get back their jobs.
Daniel Naujoks said Bangladesh has a very large diaspora of population spreading in different parts of the world.
Terming the migrants vulnerable people, he said the pandemic has exacerbated their conditions who usually migrate to a new place in a bid to get rid of poverty.


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I have visited York a good number of times over the years and always during the daytime, never at night. A few days ago I had the idea to go into York in the evening hoping to get shots of some of the ancient streets under the street lights. Well we arrived in York at 7 p.m and within two minutes I knew it was a mistake. I had forgotten it was a bank holiday weekend (easily done when you’re retired!) and the place was heaving with people, many of whom were well on the way to wearing their wobbly boots. To be honest I didn’t feel at all comfortable and eventually headed for the relative tranquility of the city walls as the light was fading. I took these two pictures as we walked along the wall heading for where it crosses Micklegate near to where we left the car and headed back into the countryside.
I love that expression ‘wobbly boots’! I particularly like the second image. Well chosen viewpoint and the processing is unusual but really very effective. It reminds me of an engraving. Clever work.
Yes . . I’ve worn wobbly boots a time or two but I have hung them up these days. I liked the viewpoint for the second image but I think I went a bit mad in post with it! The light was quite poor and I will re-visit this one at some other time to see if I can do better.

domain: travel


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Writing in Pharmacological Research, the researchers pointed out that the improvement was accompanied with shifts in the study participants’ intestinal microbiota, albeit not a drastic one.
A total of 163 patients with chronic constipation were involved into the 42-day clinical trial.
They were randomised assigned to the intervention or placebo group, where the intervention group took in one sachet of Lactiplantibacillus plantarum P9 powder per day after meal.
This is a proprietary strain of Chinese firm CR Jiangzhong (China Research Jiangzhong) and is isolated from traditional acidic gruel. According to the company’s website, The strain is already used in the company’s solid beverage sold in a box of 20 sachets each weighing two grams.
All participants had to complete an electronic stool diary to record their bowel frequency, as well as answer questionnaires pertaining to their quality of life.
Stool samples were also collected at baseline, day 28 and the last day of the trial.
Results showed that after 28 days of intervention, weekly mean complete spontaneous bowel movements (CSBMs) and spontaneous bowel movements (SBMs) of the intervention group were significantly higher.
Specifically, their weekly mean frequencies of CSBMs and SBMs were respectively 28 per cent and 12 per cent higher than the placebo.
At day 42, the weekly mean frequency of CSBMs in the intervention group remained significantly higher than the placebo group and was two-fold higher as compared to baseline levels.
However, other parameters, such as weekly mean score of stool consistency and mean score of stool straining, did not show any significant differences between the two groups.
As for quality of life (QOL), the subscore of worries and concerns in the intervention group was 1.22-fold significantly lower than the placebo at day 14 of the trial.
No significant difference was reported in other QOL and psychological parameters measured, namely physical discomfort, psychosocial discomfort, satisfaction, depression, anxiety, and stress levels.
Fine-tuning, not drastic change in gut microbiota
The improvements seen in the intervention group were the results of the “fine tuning of certain functional intestinal microbiota and microbial metabolites”, instead of drastic changes, the researchers highlighted.
The researchers said so because firstly, there was no significant differences in the gut microbiota structure between the two groups, which suggests “the absence of obvious changes in the overall gut bacteria microbiota structure regardless of probiotic consumption.”
Analysis also showed that the overall bacteriophage community in the intervention and placebo groups did not show obvious structural difference, suggesting that probiotic administration did not cause noticeable change in the overall bacteriophage community.
However, there were significant changes seen in certain functional intestinal microbiota and clinical outcomes.
As compared to the placebo group, the intervention group was significantly enriched in potentially beneficial bacteria such as Lactiplantibacillus plantarum and Ruminococcus_B gnavus, while depriving of several bacterial and phage taxa such as Oscillospiraceae sp., Lachnospiraceae sp., and Herelleviridae.
Oscillospiraceae sp, for example, is inversely proportionate to spontaneous bowel movement.
“Our results strongly supported that the application of P9 could effectively alleviate constipation, and the symptom relief effects were linked to desired changes and interactions with different types of host microbes, including the gut commensal bacteria (L. plantarum, Ruminococcus_B gnavus, Oscillospiraceae sp., Lachnospiraceae sp.) and the bacteriophage family, Herelleviridae,” said the researchers.
Amino acid and SCFA metabolic pathways
Changes were also seen in the gut microbial metabolism, especially in amino acid and short-/medium-chain fatty acids metabolic pathways.
For instance, the intervention group had significantly more beneficial microbial metabolites, such as fatty acid valeric acid, amino acids L-asparagine, and L-cysteine at day 28; and significantly more medium-chain fatty acid caprylic acid and L-pipecolinic acid – metabolite of lysine at day 42.
This indicated “the modulation in lysine degradation, phenylalanine metabolism, and other amino acid metabolic pathways” post probiotic intervention, the researchers pointed out.
“In short, the constipation relief effect of P9 intervention was accompanied by desirable changes in the fecal metagenome and metabolome. Our findings support the notion of applying probiotics in managing CC (chronic constipation),” the researchers concluded.
Source: Pharmacological Research
Effect of the probiotic strain, Lactiplantibacillus plantarum P9, on chronic constipation: A randomized, double-blind, placebo-controlled study
Authors: Teng Ma et al

domain: medical


text:
The Strain 2014 Comic-Con mini poster
Item Number: strainposter
Roughly 11 by 17 inch mini poster distributed at 2014 Comic-Con to promote the new FX horror series The Strain created by Guillermo del Toro and Chuck Hogan.
A written certificate of authenticity is provided with each autographed collectible, as required by law.
All autographs are guaranteed authentic for LIFE. Autographed baseballs, golf balls and pucks come with free plastic holders.


Manchester Code Decoding Apparatus.
DEPARTMENT OF THE NAVY WASHINGTON DC
Pagination or Media Count:
An apparatus for decoding a Manchester encoded waveform is described in which a gating circuit responds to the mid-cell transitions in the encoded waveform to produce an enabling signal which causes a clock circuit to generate high frequency clock pulses. A programmable counter accumulates the generated clock pulses. If the counter exceeds a clock count threshold set by a multiposition switch before the beginning of the following enabling signal, it causes a storage element to sample the encoded waveform and store the sample to provide an output signal representing decoded data. A second, substantially equivalent circuit decodes timing from the encoded waveform. The multiposition switch provides the apparatus with the capability to decode encoded waveforms of varying frequency.
- Electrical and Electronic Equipment


I created 3D graph in plotly to represent a data set with two classes. I used 3D scatter for visualizing data points. I divided the data set into three sets by using two 3D surfaces. I want to show cross sections of Z axis using a slider like in this example.
Visualizing MRI volume slices
Is there any way or any reference will helps to me.


I'm a violinist who reads everything! Science fiction opens the mind to possibilities, nonfiction opens the mind to knowledge, and art opens the mind to meaning.
Meaning can be sought and glimpsed through many mediums, each a unique, clouded stained glass window into the soul. Books are wonderful, but music and art can communicate too.
Through the month of July I am studying at a chamber music festival where I am surrounded by this wonderful atmosphere of brilliant, caring professors, motivated students, amazing music, and an idyllic Vermont summer. It has allowed me to reflect on the role music plays in my life. I've concluded that music—not books—is the primary medium through which I derive and communicate meaning.
For my chamber music project, I am composing a piece for violin and voice based on Maya Angelou's poem "Caged Bird." I figured with all the recent news surrounding race-motivated killings, this was an appropriate choice for a text (and it also happens to be one of my personal favorites). As a composer, my function is to communicate ideas through sounds, similarly to how a writer uses words. With music, I can add another dimension of the anguish Angelou conveyed, in the form of sound.
Gustav Mahler said, "Where words fail, music speaks." This is why live performances are so important, because a performer communicates with the audience. (In the classical tradition, the performer relays a message written by the composer and conveys it to the audience, interpreting and translating as they go along.)
The ground is fertile with the language of emotion. Let's not forget the role art and music play in communicating ideas!


The FCC voted Thursday to repeal the Obama-era classification that declared the internet as a public utility which barred providers from charging premiums for select content or websites.
A 3-2 vote by FCC Commissioners along party lines decided the repeal.
The vote to repeal the 2015 net neutrality rules comes after President Trump's appointed FCC Chairman Ajit Pai argued in May that the rules hamper broadband investment. Instead, he introduced a repeal proposal as a way stop the federal government from "micromanaging the internet."
Under the rules of net neutrality, ISPs may not charge customers premium prices to use movie-streaming sites or pages that promote a specific agenda.
Supporters of an open internet claim it keeps the web accessible to all people, regardless of their socioeconomic status. Without these rules, they believe ISPs could censor content or charge additional fees for usage at their own discretion.
Among those supporters are some of the country's largest web companies, including Google, Netflix and Twitter, who believe internet users should be given free reign to the web without fear of fees.
Opponents of net neutrality argue that the rules are unnecessary, squashing potential job creation and free market competition from large and small companies alike.
Among those who oppose the FCC's current regulations are big name service providers, including AT&T and Verizon. Both claim that while they support an open web space, the public utility classification does not ultimately support a free internet.0comments
Regardless of the FCC's decision, many service providers have worked to assure customers that their internet coverage would largely remain the same.
In a blog post this week, Comcast said its customers "will continue to enjoy all the benefits of an open Internet today, tomorrow, and in the future. Period."


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The Pittsburgh Steelers’ Week 18 game against the Cleveland Browns will not be available on television for most of the country.
The Steelers and Browns will be televised in the Pittsburgh, Erie and Scranton media markets in Pennsylvania, Northeast Ohio, most of West Virginia and parts of the Carolinas.
With the nation understandably occupied with the story of the Buffalo Bills following the hospitalization of Damar Hamlin last week, most of the country will see the Bills plays the New England Patriots on CBS at 1 p.m.
Of course, that game is still important to Steelers fans, as the Steelers need the Bills to beat the Patriots in order to clinch a playoff berth.
The other game the Steelers need a result in is the New York Jets at the Miami Dolphins. That game is on FOX and will be shown in Pittsburgh on WPGH-TV. It will also be shown throughout most of the Northeast on FOX affiliates.
Pittsburgh will also get the late CBS game between the New York Giants and Philadelphia Eagles, with Philly looking to wrap up the NFC East. On WPGH, the late game will be the Dallas Cowboys at the Washington Commanders.
Sunday Night Football on WPXI will have the Detroit Lions and the Green Bay Packers, with a Wild Card berth on the line for Aaron Rodgers and company.


Adaptive Synapse Arrangement in Cortical Learning Algorithm
Takeru Aoki, Keiki Takadama, and Hiroyuki Sato
Graduate School of Informatics and Engineering, The University of Electro-Communications
1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan
The cortical learning algorithm (CLA) is a time-series data prediction method that is designed based on the human neocortex. The CLA has multiple columns that are associated with the input data bits by synapses. The input data is then converted into an internal column representation based on the synapse relation. Because the synapse relation between the columns and input data bits is fixed during the entire prediction process in the conventional CLA, it cannot adapt to input data biases. Consequently, columns not used for internal representations arise, resulting in a low prediction accuracy in the conventional CLA. To improve the prediction accuracy of the CLA, we propose a CLA that self-adaptively arranges the column synapses according to the input data tendencies and verify its effectiveness with several artificial time-series data and real-world electricity load prediction data from New York City. Experimental results show that the proposed CLA achieves higher prediction accuracy than the conventional CLA and LSTMs with different network optimization algorithms by arranging column synapses according to the input data tendency.
-  J. Hawkins, A. Subutai, and D. Dubinsky, “Hierarchical temporal memory including HTM cortical learning algorithms,” Technical report, Numenta, Inc., 2010.
-  J. Hawkins and A. Subutai, “Why Neurons Have Thousands of Synapses, a Theory of Sequence Memory in Neocortex,” Frontiers in Neural Circuits, Vol.10, pp. 1-13, 2016.
-  NuPIC, https://github.com/numenta/nupic [accessed February 27, 2020]
-  J. Hawkins and S. Blakeslee, “On Intelligence,” Times Books, 2004.
-  S. Ahmad and J. Hawkins, “Properties of Sparse Distributed Representations and their Application to Hierarchical Temporal Memory,” Numenta, pp. 1-18, 2015.
-  L. R. Rabiner and B. H. Juang, “An Introduction to Hidden Markov Models,” IEEE Acoustics, Speech & Signal Processing Magazine, Vol.3, No.1, pp. 4-16, 1986.
-  L. R. Rabiner, “A Tutorial on Hidden Markov Models and Selected Applications in Speech Recognition,” Proc. of the IEEE, Vol.77, No.2, pp. 257-285, 1989.
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This article is published under a Creative Commons Attribution-NoDerivatives 4.0 Internationa License.


text:
Welcome to the heart-pounding world of Slope Tunnel, a visually stunning endless runner game that will leave you breathless. In this thrilling game, you’ll guide a ball-like character through a vibrant neon tunnel, dodging obstacles and collecting gems. Get ready for an adrenaline-pumping gaming experience that will test your skills and keep you coming back for more.
Master the Game Controls
To conquer the neon-infused tunnel in Slope Tunnel, you need to master the game controls. Here’s what you need to know:
- Keyboard: Use the arrow keys to control your character’s movement. Steer left and right to change direction. Press the “P” key to pause the game.
How to Play Slope Tunnel
Embarking on a high-speed journey through the neon tunnel is easier than you think. Follow these steps:
Access the Game: Visit trusted gaming websites to find Slope Tunnel and start your adventure.
Choose Your Difficulty: Select your preferred difficulty level: Easy, Normal, Hard, or Extreme. Each level offers unique challenges.
Navigate the Neon Tunnel: Once the game starts, your character will roll down the neon tunnel. Your goal is to steer clear of obstacles and survive as long as possible.
Steer with Precision: Use the arrow keys to navigate through the tunnel. Be careful of red barriers and gaps, as colliding with them will end the game.
Collect Gems: Keep an eye out for green gems scattered along the way. Collecting them adds to your score, but be prepared for challenging positions that require precise control.
Survival is Key: In Slope Tunnel, there’s no defined endpoint. Focus on achieving the highest score and surpassing your previous records.
Challenge Yourself: Use the fast-paced nature of Slope Tunnel to challenge yourself, beat your high scores, and refine your skills.
Tips and Tricks
To become a pro in Slope Tunnel, consider these tips:
Maintain a steady pace: Rushing through the tunnel may tempt you, but maintaining a consistent speed will help you respond better to obstacles.
Prioritize precision: The game’s controls demand precise movements. Take your time to navigate around obstacles with accuracy.
Don’t forget to pause: If you need a moment to strategize or catch your breath, press the “P” key to pause the game.
Meet the Game Developer
Slope Tunnel was developed by Rob Kay, an indie game developer known for creating visually captivating and fast-paced 3D running games.
Slope Tunnel is available on mobile devices! Download the game on iOS and Android devices and enjoy the thrill on the go.
In conclusion, Slope Tunnel offers a visually stunning and pulse-pounding gaming experience. Navigate the neon-infused tunnel, dodge obstacles, and collect gems as you strive to beat your high scores and challenge your friends. Prepare for the neon rush and tackle the tunnel in Slope Tunnel!

domain: game


View and Download HP LaserJet P Series service manual online. LaserJet P Series. LaserJet P Series All in One Printer pdf manual download. HP DN MANUAL HP LASERJET P — | PDF | KB | 02 Oct HP DN MANUAL HP LASERJET P INTRODUCTION This. Click the “TCP/IP” tab and use the drop-down box to change the configuration method to “Manual.” Type the new IP address and click the “Apply” button.
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Insert the left side of the pickup roller into the left slot 1and insert the right side the side that has the notches in the shaft into the right slot 2. Place hp2015xn product on an ESD mat.
HP LaserJet P2015dn Printer User Guides
Figure Removing the duplexer tray Pull out the duplexer tray. The leading edge janual the paper should have advanced past the print cartridge.
Disconnect one flat, flexible cable 2 at the top of the formatter. Do not use paper that is embossed or coated and is not designed for the temperatures of the printer’s image fuser.
HP LaserJet Pdn Printer User Guides | HP® Customer Support
Each process functions independently and must be coordinated with the other printer processes. Europe, Middle East, Africa. Does the printed page look like you expected?
Processing Data The printer is processing or receiving data.
Replacing the Formatter HP LaserJet P PD PN PDN
This product has been exchanged for a new or refurbished product. Mnual Problemsolving Media problemsolving The following problems with media cause print-quality deviations, jamming, or damage to the printer.
Page 90 The laser beam scans the photosensitive drum to neutralize negative charges on parts of the drum. Plug the printer directly into an AC outlet instead of into a power strip. The light pattern changes.
A small amount of toner scatter is normal for laser printing. Reinstall the print cartridge, and close the print-cartridge door. Press the Go button to override the condition. View printer status information.
Light print or faded The print cartridge is nearing end of life. Page Open the straight-through output door. Page – Hewlett-Packard software license agreeme Squeeze the left side of the pickup-roller cover up2015dn insert the left retaining tab in the retaining slot. Don’t show me this message again. Page it back in and turn the power on. If the recovery is successful, the printer goes to the Manual feed Accessory Error To display gp2015dn error information, press the button.
HP LaserJet P2015 Series Service Manual
Pull the duplexer tray release-tab 1 to release the duplexer tray magnets. Release the control-panel cable 1 on the left side of the printer. With both hands, grasp the side of the media that is most visible this includes the middleand carefully pull it free from the printer. ENWW Replace the pickup roller tray 1.
In your printer driver, make sure the appropriate media type is selected. Over time, this buildup can cause print quality problems, such as toner specks or smearing, and paper jams. Figure Removing the fan 1 of 2 Unthread the fan wires from the retaining clips.
Toner Missing The print cartridge has been removed from the printer. Image Problem Solving Image problem solving This section provides information about identifying and correcting print defects.
Maintenance Replace the print cartridge Redistribute toner Clean the printer Clean the pickup roller tray 1 Replace the pickup roller tray 1 Clean the pickup roller tray 2 Replace the pickup roller tray 2 ENWW Download list – Download multiple files at once View, edit and download your entire list of software and drivers here.
How does HP install software and gather data? The laser beam scans the photosensitive drum to neutralize negative charges on parts of the drum.
Checking the high-voltage connector assembly Visually inspect the cartridge connection points inside the printer to make sure that they are not dirty or corroded. Top cover Remove the left-side cover, the right-side cover, the back cover, and the duplexer tray.
Main motor Remove all covers. You may not disassemble or decompile the Software unless HP’s prior written consent is obtained.


text:
Calling all artists! South Shore Tiki Lounge is hosting a nature-inspired art/photo show themed “Wild + Wicked” in support of Plant a Wish. (plantawish.org). Plant a Wish is a native tree planting awareness project holding native tree planting ceremonies in all 50 states, and will culminate in a documentary film.
Think creatures of the night and underground. Moonbows, not rainbows. The dark mysteries found in the wild. If you are interested in participating, email email@example.com. Pieces will be showcased at South Shore Tiki Lounge for the month of November with an opening Wild + Wicked costume party on Sunday October 30th.
A portion of proceeds or full donation of any art sold during the exhibit will be donated to Plant a Wish as well as 10% of dinner sales on opening night. The Plant a Wish tour closes out right here in Maui on November 5th so let’s celebrate Sara and Joe’s remarkable accomplishment and help them reach their goal!


text:
The audio reveals that Cory Monteith as Finn raps in Jay-Z's place, assisted by Amber Riley and her soulful voice in Alicia Key's part.
One week away from the season 2 premiere, "Glee" tides fans over with the audio of "Empire State of Mind" cover. Debuted via Sirius XM radio on Tuesday, September 14, the song originally sung by Jay-Z is notably rapped by Kevin McHale's Artie, Cory Monteith's Finn and Mark Salling's Puck. Meanwhile, Alicia Keys' part is tackled by Amber Riley's Mercedes.
The cover is picked as the club is heading to Nationals in NYC after placing at Regionals. A promo containing the performance scene from the first return episode has been released, revealing at the same time that the Gleeks would wear "New York City" shirts complete with the Hip-Hop attribute.
Rolling Stone reported that "Auditions" will also feature renditions of Lady GaGa's "Telephone," Travis McCoy's "Billionaire," and Poison's "Every Rose Has Its Thorn." They will additionally do Broadway numbers like "What I Did for Love" from "A Chorus Line" and "Getting to Know You" from "The King and I", as well as "Listen" from the film version of "Dreamgirls".
However, "Empire State of Mind" could be the season 2 anthem. FOX marketing president Joe Earley said, "There's a good chance we'll be hearing the song again."


text:
5.0 out of 5 stars Effective insights, reminders, and a practical path to self-esteem August 6, 2014 By a customer
I highly recommend this book. Potential benefits include healthier relationships, success in business, and improved emotional health. Following Darlene Lancer’s Ten Steps to Self-Esteem will increase self-esteem. It offers a minimum of theory and explanation with a maximum of practical steps. For me, however, the book’s value went beyond that. The idea of treating a self-critic as another person, turning an adversary into a friend and coach, was a new idea for me. So was recognizing a self critic as potentially a well-intentioned entity that needed understanding and compassion. I think the most important aspect of Lancer’s advice for dealing with a self-critic is to evaluate the critic’s criticism. What I learned from Lancer is that rhymes “integrate the brain’s right and left hemispheres to enhance holistic brain function.” I also learned that adding memory can “stimulate emotion and evoke alpha and theta non-ordinary brain waves that increase memory and learning.”
5.0 out of 5 stars Five Stars February 5, 2017 By Lacy Nummer
I believe this can be used in my private practice.
5.0 out of 5 stars Step by step Improve your Self-Esteem, Highly recommended August 15, 2016 By Perry
Follow step by step to Improve your Self Esteem. Highly recommended.
5.0 out of 5 stars Simple and Positive Dec. 1, 2016 By ZenKen212
I really can’t find anything wrong with this book, which is surprising considering my internal critic is excellent in finding fault with a whole array of things. This is a good one. This step by step method couldn’t be simpler.
5.0 out of 5 stars Five Stars July 20, 2016 By Vernon Banks
5.0 out of 5 stars Helpful October 22, 2016 By Angela M. Dokos
This is a short and to the point book. It’s helpful and encouraging.

domain: literature


WW2 Men's Jeans - Deadstock Blue (1944) | S1000XX
The culmination of Warehouse's 25-year experience in denim production. Made to fade like vintage jeans. This is achieved through the oxidation of the indigo dye, imparting the jean with a distinct shade of blue only seen in deadstock jeans which have spent decades unused and in storage. These jeans are raw and will shrinkage to tag size through the wash.
- Choosing a selection results in a full page refresh.
- Press the space key then arrow keys to make a selection.


text:
First Aid Kit Sign - Laser Engraved
Mark first aid kits with a simple, effective tent sign.
First aid signs are made with durable acrylic material and engraved with crisp text and icons. Easy to read first aid kit signs for your office, school, restaurant, or medical facility.
Customize colors on your first aid sign to match your needs. Table and counter signs from Office Sign Company.
If you don't see a size you need or require a custom design, please contact us at (701) 526-3835 or at email@example.com with all of your details.
- Shipping, Returns, and Guarantees
First Aid Sign - Tent Table Signs

domain: design


text:
Bethesda Softwoorks has released a new video featuring developer commentary for the upcoming DOOM game showcasing one of the title’s most exciting new features, the SnapMap. You can check out the new video in the player below.
DOOM’s new SnapMap allows players to create their own gameplay maps for DOOM. id Software Executive Producer Marty Stratton and Global Content Manager Gary Steinman break down the feature and what it has to offer players. It is a content-creation tool that will work on all the versions the game. Players can even access creations from other gamers in the DOOM community. In the game, they can alter the environment how they see fit. In short, players will be able to create their own vision of DOOM and share it with others.
With the popularity of games such as Minecraft, a community and content-creation tool for the DOOm franchise is definitely an exciting prospect. It will be interesting to see how far players can go with the level and map creation with the SnapMap feature. In addition, one imagines what bizarre creations players might cook up as well.
Doom is set for a second quarter 2016 release date. The game will be available for PlayStation 4, Xbox One and Windows PC.

domain: entertainment


text: did anyone else cry like a little baby at the end? This sequel really exceeded my expectations greatly. i was almost expecting crap, but what i was given was an animated 3d masterpiece that makes you laugh, cry, and occasionally make you fear for the toys. my only problems were, no little Bo Peep, and, ( you may think i am a jerk for this) if the toys died at the end i would have been sadder, but it would make more sense for the letting go of your childhood part of the storyline, don't get me wrong, i love the originals and the current ending, it just would have made more sense with the storyline. thank you cast, crew and others, for this wonderful tear jerking, sometimes dark, masterpiece, about letting go of your childhood.


text:
NH flu deaths increase to 16
Beth Daly, chief of infectious disease surveillance for the state Department of Health and Human Services, said two more deaths were reported since Friday, when the number of adult influenza-related deaths reported to the state stood at 14.
Join the discussion below, or Read more at Cw56.com.
Since: Jan 13
#1 Jan 16, 2013
Never fails I always get the flu each year. However I have this great service MD247. I was sick over Christmas. I woke up Christmas Eve morning and felt awful. Stuffy nose, sore throat, chills and could not hold anything down. I called MD247 and spoke with a Doctor. He called me in a prescription to my pharmacy, by Christmas morning I was feeling better. I could actually enjoy the time with my family. www.md247.com
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