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“A memoir is a narrative, written from the perspective of the author, about an important part of their life.” - Celadon Books.
And yet, often when we think of memoirs, we think of celebrities or renowned figures. We think of people reflecting back on their life once it has been lived.
This is why we’re surprised to hear of memoirs written by younger people, such as 22-year-old Molly-Mae, or people that weren’t famous before they told their story. As time progresses, the boundaries to memoirs are lessened, and within the pile, we find some interesting ones.
It’s unconventional memoirs that are often the best ones. Whilst these ‘traditional’ memoirs can follow such a strict outline, with the right amount of secrets or reveals, it’s unconventional memoirs that actually challenge the way we think and feel. It’s unconventional memoirs that we can relate to, as inside we’re all writing our own.
I recommend this book a lot, and I don’t think that will stop anytime soon. I like to describe it as the memoir of an everyday girl. This book is what made people know Dolly Alderton, rather than traditional memoirs where they’re writing about their famous life.
This makes it so relatable, as Dolly speaks of MSN relationship, parties at university, watching friends get engaged and more. I recently reread this book and was struck by how it’s also a love letter to being single and discovering your identity. I think the point in life you’re at when reading this book will impact what you take from it, but there are always lesson to be learned.
I thought that this would be a traditional book on writing, but I was pleasantly surprised to find over half the book focused on his own life and experiences. There was even an updated section to include his accident, which happened when writing this very book.
I think Stephen aims to show that if he can be a writer, anyone can. If he can make it, why can’t you? It’s a book about perseverance, life lessons and acknowledging everyone who helped you along the way. It’s an untraditional memoir as it’s also a guide to writing, not just how to get published, but how to actually write a half-decent book. Stephen considers both the larger aspects of writing routine and querying, as well as going as specific as sentence structure and useless words. A must-read for any aspiring writer, or even published ones.
This book differs to the others listed in that I was already acquainted with Rachel Bloom before reading it. In fact, I was counting down the days until the release of her memoir.
I’m a big fan of her show Crazy Ex-Girlfriend, particularly for how it portrays Borderline Personality Disorder. Rachel not only created and wrote the show and music, but also starred in it as Rebecca Bunch. I really recommend giving it a go! You’ll find it on Netflix.
This is a memoir about being strange. It’s about being the person who never quite fit in. Rachel lays it all on the table, including mental health, potty training, hiding in bathrooms and more. It’s a collection of essays that take you from childhood to her time on Crazy Ex-Girlfriend and further.
Rachel jumps between styles and timelines, mimicking the way a frazzled mind works. It’s fun, easy and managed to be both relatable and utterly odd.
It almost feels wrong to call this book a memoir, as it read like a story, so beautiful that it had to be made up. Just Kids starts and ends with Robert’s death, and then takes you through the heartwrenching in-between. It’s a tale of love, art, hope and so much more.
It’s a love story, an unconventional one for sure, but a love story nonetheless. This book felt like a secret you clutch close to your heart and refuse to let go. It’s easy to recognise Patti as a poet through her delicate prose, and yet she also takes us along the adventure and keeps the reader enthralled.
When I think of a memoir, it’s usually in terms of someone famous or important, often in an artistic or political setting. In recent years, we’ve moved away from this rigid definition to include unconventional memoirs, and Educated is definitely one of these.
Tara was raised in the mountains of Idaho to survivalist parents. She never went to school or a hospital, she was isolated from the world. She was seventeen the first time she entered a classroom.
And yet, against all odds, Tara managed to leave her home behind and embark on a fierce quest for knowledge. Tara explores her own history and what it means to be educated.
I read this book quickly, and I haven’t stopped thinking about it since.
Whilst I’m usually much more of a fiction reader, these were the memoirs that tempted me into a new genre, and that will make me search for more. They showed me that memoir authors don’t have to be famous before writing their book, and a good one will become famous from it.
Have I missed any unconventional memoirs that you enjoyed? Let me know, I’m always looking for books to read next.
*This article contains affiliate links.
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Gas chromatography (GC) is a highly sensitive method used to identify and quantify the fatty acid content of lipids from tissues, cells, and plasma/serum, yielding results with high accuracy and high reproducibility. In metabolic and nutrition studies GC allows assessment of changes in fatty acid concentrations following interventions or during changes in physiological state such as pregnancy. Solid phase extraction (SPE) using aminopropyl silica cartridges allows separation of the major lipid classes including triacylglycerols, different phospholipids, and cholesteryl esters (CE). GC combined with SPE was used to analyze the changes in fatty acid composition of the CE fraction in the livers of virgin and pregnant rats that had been fed various high and low fat diets. There are significant diet/pregnancy interaction effects upon the omega-3 and omega-6 fatty acid content of liver CE, indicating that pregnant females have a different response to dietary manipulation than is seen among virgin females.
19 Related JoVE Articles!
Identification of Metabolically Active Bacteria in the Gut of the Generalist Spodoptera littoralis via DNA Stable Isotope Probing Using 13C-Glucose
Institutions: Max Planck Institute for Chemical Ecology.
Guts of most insects are inhabited by complex communities of symbiotic nonpathogenic bacteria. Within such microbial communities it is possible to identify commensal or mutualistic bacteria species. The latter ones, have been observed to serve multiple functions to the insect, i.e.
helping in insect reproduction1
, boosting the immune response2
, pheromone production3
, as well as nutrition, including the synthesis of essential amino acids4,
Due to the importance of these associations, many efforts have been made to characterize the communities down to the individual members. However, most of these efforts were either based on cultivation methods or relied on the generation of 16S rRNA gene fragments which were sequenced for final identification. Unfortunately, these approaches only identified the bacterial species present in the gut and provided no information on the metabolic activity of the microorganisms.
To characterize the metabolically active bacterial species in the gut of an insect, we used stable isotope probing (SIP) in vivo
C-glucose as a universal substrate. This is a promising culture-free technique that allows the linkage of microbial phylogenies to their particular metabolic activity. This is possible by tracking stable, isotope labeled atoms from substrates into microbial biomarkers, such as DNA and RNA5
. The incorporation of 13
C isotopes into DNA increases the density of the labeled DNA compared to the unlabeled (12
C) one. In the end, the 13
C-labeled DNA or RNA is separated by density-gradient ultracentrifugation from the 12
C-unlabeled similar one6
. Subsequent molecular analysis of the separated nucleic acid isotopomers provides the connection between metabolic activity and identity of the species.
Here, we present the protocol used to characterize the metabolically active bacteria in the gut of a generalist insect (our model system), Spodoptera littoralis
). The phylogenetic analysis of the DNA was done using pyrosequencing, which allowed high resolution and precision in the identification of insect gut bacterial community. As main substrate, 13
C-labeled glucose was used in the experiments. The substrate was fed to the insects using an artificial diet.
Microbiology, Issue 81, Insects, Sequence Analysis, Genetics, Microbial, Bacteria, Lepidoptera, Spodoptera littoralis, stable-isotope-probing (SIP), pyro-sequencing, 13C-glucose, gut, microbiota, bacteria
Isolating and Using Sections of Bovine Mesenteric Artery and Vein as a Bioassay to Test for Vasoactivity in the Small Intestine
Institutions: USDA-Agricultural Research Service.
Mammalian gastrointestinal systems are constantly exposed to compounds (desirable and undesirable) that can have an effect on blood flow to and from that system. Changes in blood flow to the small intestine can result in effects on the absorptive functions of the organ. Particular interest in toxins liberated from feedstuffs through fermentative and digestive processes has developed in ruminants as an area where productive efficiencies could be improved. The video associated with this article describes an in vitro
bioassay developed to screen compounds for vasoactivity in isolated cross-sections of bovine mesenteric artery and vein using a multimyograph. Once the blood vessels are mounted and equilibrated in the myograph, the bioassay itself can be used: as a screening tool to evaluate the contractile response or vasoactivity of compounds of interest; determine the presence of receptor types by pharmacologically targeting receptors with specific agonists; determine the role of a receptor with the presence of one or more antagonists; or determine potential interactions of compounds of interest with antagonists. Through all of this, data are collected real-time, tissue collected from a single animal can be exposed to a large number of different experimental treatments (an in vitro
advantage), and represents vasculature on either side of the capillary bed to provide an accurate picture of what could be happening in the afferent and efferent blood supply supporting the small intestine.
Medicine, Issue 92, Blood flow, bovine, mesenteric artery, mesenteric vein, small intestine, vasoactivity, vasoconstriction
Fat Preference: A Novel Model of Eating Behavior in Rats
Institutions: University of Texas Medical Branch.
Obesity is a growing problem in the United States of America, with more than a third of the population classified as obese. One factor contributing to this multifactorial disorder is the consumption of a high fat diet, a behavior that has been shown to increase both caloric intake and body fat content. However, the elements regulating preference for high fat food over other foods remain understudied.
To overcome this deficit, a model to quickly and easily test changes in the preference for dietary fat was developed. The Fat Preference model presents rats with a series of choices between foods with differing fat content. Like humans, rats have a natural bias toward consuming high fat food, making the rat model ideal for translational studies. Changes in preference can be ascribed to the effect of either genetic differences or pharmacological interventions. This model allows for the exploration of determinates of fat preference and screening pharmacotherapeutic agents that influence acquisition of obesity.
Behavior, Issue 88, obesity, fat, preference, choice, diet, macronutrient, animal model
Experimental Protocol for Manipulating Plant-induced Soil Heterogeneity
Institutions: Case Western Reserve University.
Coexistence theory has often treated environmental heterogeneity as being independent of the community composition; however biotic feedbacks such as plant-soil feedbacks (PSF) have large effects on plant performance, and create environmental heterogeneity that depends on the community composition. Understanding the importance of PSF for plant community assembly necessitates understanding of the role of heterogeneity in PSF, in addition to mean PSF effects. Here, we describe a protocol for manipulating plant-induced soil heterogeneity. Two example experiments are presented: (1) a field experiment with a 6-patch grid of soils to measure plant population responses and (2) a greenhouse experiment with 2-patch soils to measure individual plant responses. Soils can be collected from the zone of root influence (soils from the rhizosphere and directly adjacent to the rhizosphere) of plants in the field from conspecific and heterospecific plant species. Replicate collections are used to avoid pseudoreplicating soil samples. These soils are then placed into separate patches for heterogeneous treatments or mixed for a homogenized treatment. Care should be taken to ensure that heterogeneous and homogenized treatments experience the same degree of soil disturbance. Plants can then be placed in these soil treatments to determine the effect of plant-induced soil heterogeneity on plant performance. We demonstrate that plant-induced heterogeneity results in different outcomes than predicted by traditional coexistence models, perhaps because of the dynamic nature of these feedbacks. Theory that incorporates environmental heterogeneity influenced by the assembling community and additional empirical work is needed to determine when heterogeneity intrinsic to the assembling community will result in different assembly outcomes compared with heterogeneity extrinsic to the community composition.
Environmental Sciences, Issue 85, Coexistence, community assembly, environmental drivers, plant-soil feedback, soil heterogeneity, soil microbial communities, soil patch
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Institutions: National Research Council Canada.
One of the major questions in microbial ecology is “who is there?” This question can be answered using various tools, but one of the long-lasting gold standards is to sequence 16S ribosomal RNA (rRNA) gene amplicons generated by domain-level PCR reactions amplifying from genomic DNA. Traditionally, this was performed by cloning and Sanger (capillary electrophoresis) sequencing of PCR amplicons. The advent of next-generation sequencing has tremendously simplified and increased the sequencing depth for 16S rRNA gene sequencing. The introduction of benchtop sequencers now allows small labs to perform their 16S rRNA sequencing in-house in a matter of days. Here, an approach for 16S rRNA gene amplicon sequencing using a benchtop next-generation sequencer is detailed. The environmental DNA is first amplified by PCR using primers that contain sequencing adapters and barcodes. They are then coupled to spherical particles via emulsion PCR. The particles are loaded on a disposable chip and the chip is inserted in the sequencing machine after which the sequencing is performed. The sequences are retrieved in fastq format, filtered and the barcodes are used to establish the sample membership of the reads. The filtered and binned reads are then further analyzed using publically available tools. An example analysis where the reads were classified with a taxonomy-finding algorithm within the software package Mothur is given. The method outlined here is simple, inexpensive and straightforward and should help smaller labs to take advantage from the ongoing genomic revolution.
Molecular Biology, Issue 90, Metagenomics, Bacteria, 16S ribosomal RNA gene, Amplicon sequencing, Next-generation sequencing, benchtop sequencers
Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
Institutions: San Diego State University, DOE Joint Genome Institute, University of Colorado, University of Colorado.
The accessibility of high-throughput sequencing has revolutionized many fields of biology. In order to better understand host-associated viral and microbial communities, a comprehensive workflow for DNA and RNA extraction was developed. The workflow concurrently generates viral and microbial metagenomes, as well as metatranscriptomes, from a single sample for next-generation sequencing. The coupling of these approaches provides an overview of both the taxonomical characteristics and the community encoded functions. The presented methods use Cystic Fibrosis (CF) sputum, a problematic sample type, because it is exceptionally viscous and contains high amount of mucins, free neutrophil DNA, and other unknown contaminants. The protocols described here target these problems and successfully recover viral and microbial DNA with minimal human DNA contamination. To complement the metagenomics studies, a metatranscriptomics protocol was optimized to recover both microbial and host mRNA that contains relatively few ribosomal RNA (rRNA) sequences. An overview of the data characteristics is presented to serve as a reference for assessing the success of the methods. Additional CF sputum samples were also collected to (i) evaluate the consistency of the microbiome profiles across seven consecutive days within a single patient, and (ii) compare the consistency of metagenomic approach to a 16S ribosomal RNA gene-based sequencing. The results showed that daily fluctuation of microbial profiles without antibiotic perturbation was minimal and the taxonomy profiles of the common CF-associated bacteria were highly similar between the 16S rDNA libraries and metagenomes generated from the hypotonic lysis (HL)-derived DNA. However, the differences between 16S rDNA taxonomical profiles generated from total DNA and HL-derived DNA suggest that hypotonic lysis and the washing steps benefit in not only removing the human-derived DNA, but also microbial-derived extracellular DNA that may misrepresent the actual microbial profiles.
Molecular Biology, Issue 94, virome, microbiome, metagenomics, metatranscriptomics, cystic fibrosis, mucosal-surface
Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology
Institutions: San Diego State University, University of California San Diego.
Here we introduce a series of thoroughly tested and well standardized research protocols adapted for use in remote marine environments. The sampling protocols include the assessment of resources available to the microbial community (dissolved organic carbon, particulate organic matter, inorganic nutrients), and a comprehensive description of the viral and bacterial communities (via direct viral and microbial counts, enumeration of autofluorescent microbes, and construction of viral and microbial metagenomes). We use a combination of methods, which represent a dispersed field of scientific disciplines comprising already established protocols and some of the most recent techniques developed. Especially metagenomic sequencing techniques used for viral and bacterial community characterization, have been established only in recent years, and are thus still subjected to constant improvement. This has led to a variety of sampling and sample processing procedures currently in use. The set of methods presented here provides an up to date approach to collect and process environmental samples. Parameters addressed with these protocols yield the minimum on information essential to characterize and understand the underlying mechanisms of viral and microbial community dynamics. It gives easy to follow guidelines to conduct comprehensive surveys and discusses critical steps and potential caveats pertinent to each technique.
Environmental Sciences, Issue 93, dissolved organic carbon, particulate organic matter, nutrients, DAPI, SYBR, microbial metagenomics, viral metagenomics, marine environment
A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples
Institutions: USDA-Agricultural Research Service, USDA-Agricultural Research Service, Oregon State University, University of Georgia, Northern Arizona University.
The efficacy of DNA extraction protocols can be highly dependent upon both the type of sample being investigated and the types of downstream analyses performed. Considering that the use of new bacterial community analysis techniques (e.g.,
microbiomics, metagenomics) is becoming more prevalent in the agricultural and environmental sciences and many environmental samples within these disciplines can be physiochemically and microbiologically unique (e.g.,
fecal and litter/bedding samples from the poultry production spectrum), appropriate and effective DNA extraction methods need to be carefully chosen. Therefore, a novel semi-automated hybrid DNA extraction method was developed specifically for use with environmental poultry production samples. This method is a combination of the two major types of DNA extraction: mechanical and enzymatic. A two-step intense mechanical homogenization step (using bead-beating specifically formulated for environmental samples) was added to the beginning of the “gold standard” enzymatic DNA extraction method for fecal samples to enhance the removal of bacteria and DNA from the sample matrix and improve the recovery of Gram-positive bacterial community members. Once the enzymatic extraction portion of the hybrid method was initiated, the remaining purification process was automated using a robotic workstation to increase sample throughput and decrease sample processing error. In comparison to the strict mechanical and enzymatic DNA extraction methods, this novel hybrid method provided the best overall combined performance when considering quantitative (using 16S rRNA qPCR) and qualitative (using microbiomics) estimates of the total bacterial communities when processing poultry feces and litter samples.
Molecular Biology, Issue 94, DNA extraction, poultry, environmental, feces, litter, semi-automated, microbiomics, qPCR
Investigating the Microbial Community in the Termite Hindgut - Interview
Institutions: California Institute of Technology - Caltech.
Jared Leadbetter explains why the termite-gut microbial community is an excellent system for studying the complex interactions between microbes. The symbiotic relationship existing between the host insect and lignocellulose-degrading gut microbes is explained, as well as the industrial uses of these microbes for degrading plant biomass and generating biofuels.
Microbiology, issue 4, microbial community, diversity
DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat
Institutions: BC Children's Hospital.
Inflammatory Bowel Diseases (IBD), including Crohn's Disease and Ulcerative Colitis, have long been associated with a genetic basis, and more recently host immune responses to microbial and environmental agents. Dinitrobenzene sulfonic acid (DNBS)-induced colitis allows one to study the pathogenesis of IBD associated environmental triggers such as stress and diet, the effects of potential therapies, and the mechanisms underlying intestinal inflammation and mucosal injury. In this paper, we investigated the effects of dietary n-3 and n-6 fatty acids on the colonic mucosal inflammatory response to DNBS-induced colitis in rats. All rats were fed identical diets with the exception of different types of fatty acids [safflower oil (SO), canola oil (CO), or fish oil (FO)] for three weeks prior to exposure to intrarectal DNBS. Control rats given intrarectal ethanol continued gaining weight over the 5 day study, whereas, DNBS-treated rats fed lipid diets all lost weight with FO and CO fed rats demonstrating significant weight loss by 48 hr and rats fed SO by 72 hr. Weight gain resumed after 72 hr post DNBS, and by 5 days post DNBS, the FO group had a higher body weight than SO or CO groups. Colonic sections collected 5 days post DNBS-treatment showed focal ulceration, crypt destruction, goblet cell depletion, and mucosal infiltration of both acute and chronic inflammatory cells that differed in severity among diet groups. The SO fed group showed the most severe damage followed by the CO, and FO fed groups that showed the mildest degree of tissue injury. Similarly, colonic myeloperoxidase (MPO) activity, a marker of neutrophil activity was significantly higher in SO followed by CO fed rats, with FO fed rats having significantly lower MPO activity. These results demonstrate the use of DNBS-induced colitis, as outlined in this protocol, to determine the impact of diet in the pathogenesis of IBD.
Medicine, Issue 84, Chemical colitis, Inflammatory Bowel Disease, intra rectal administration, intestinal inflammation, transmural inflammation, myeloperoxidase activity
High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
Institutions: Colorado State University, Oak Ridge National Laboratory, University of Colorado.
Microbes in soils and other environments produce extracellular enzymes to depolymerize and hydrolyze organic macromolecules so that they can be assimilated for energy and nutrients. Measuring soil microbial enzyme activity is crucial in understanding soil ecosystem functional dynamics. The general concept of the fluorescence enzyme assay is that synthetic C-, N-, or P-rich substrates bound with a fluorescent dye are added to soil samples. When intact, the labeled substrates do not fluoresce. Enzyme activity is measured as the increase in fluorescence as the fluorescent dyes are cleaved from their substrates, which allows them to fluoresce. Enzyme measurements can be expressed in units of molarity or activity. To perform this assay, soil slurries are prepared by combining soil with a pH buffer. The pH buffer (typically a 50 mM sodium acetate or 50 mM Tris buffer), is chosen for the buffer's particular acid dissociation constant (pKa) to best match the soil sample pH. The soil slurries are inoculated with a nonlimiting amount of fluorescently labeled (i.e.
C-, N-, or P-rich) substrate. Using soil slurries in the assay serves to minimize limitations on enzyme and substrate diffusion. Therefore, this assay controls for differences in substrate limitation, diffusion rates, and soil pH conditions; thus detecting potential enzyme activity rates as a function of the difference in enzyme concentrations (per sample).
Fluorescence enzyme assays are typically more sensitive than spectrophotometric (i.e.
colorimetric) assays, but can suffer from interference caused by impurities and the instability of many fluorescent compounds when exposed to light; so caution is required when handling fluorescent substrates. Likewise, this method only assesses potential enzyme activities under laboratory conditions when substrates are not limiting. Caution should be used when interpreting the data representing cross-site comparisons with differing temperatures or soil types, as in situ
soil type and temperature can influence enzyme kinetics.
Environmental Sciences, Issue 81, Ecological and Environmental Phenomena, Environment, Biochemistry, Environmental Microbiology, Soil Microbiology, Ecology, Eukaryota, Archaea, Bacteria, Soil extracellular enzyme activities (EEAs), fluorometric enzyme assays, substrate degradation, 4-methylumbelliferone (MUB), 7-amino-4-methylcoumarin (MUC), enzyme temperature kinetics, soil
Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans
Institutions: Washington State University, Washington State University.
Fatty acids are essential for numerous cellular functions. They serve as efficient energy storage molecules, make up the hydrophobic core of membranes, and participate in various signaling pathways. Caenorhabditis elegans
synthesizes all of the enzymes necessary to produce a range of omega-6 and omega-3 fatty acids. This, combined with the simple anatomy and range of available genetic tools, make it an attractive model to study fatty acid function. In order to investigate the genetic pathways that mediate the physiological effects of dietary fatty acids, we have developed a method to supplement the C. elegans
diet with unsaturated fatty acids. Supplementation is an effective means to alter the fatty acid composition of worms and can also be used to rescue defects in fatty acid-deficient mutants. Our method uses nematode growth medium agar (NGM) supplemented with fatty acidsodium salts. The fatty acids in the supplemented plates become incorporated into the membranes of the bacterial food source, which is then taken up by the C. elegans
that feed on the supplemented bacteria. We also describe a gas chromatography protocol to monitor the changes in fatty acid composition that occur in supplemented worms. This is an efficient way to supplement the diets of both large and small populations of C. elegans
, allowing for a range of applications for this method.
Biochemistry, Issue 81, Caenorhabditis elegans, C. elegans, Nutrition Therapy, genetics (animal and plant), Polyunsaturated fatty acids, omega-6, omega-3, dietary fat, dihomo-gamma-linolenic acid, germ cells
Polymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine?
Institutions: Cedars-Sinai Medical Center.
Tumors with similar grade and morphology often respond differently to the same treatment because of variations in molecular profiling. To account for this diversity, personalized medicine is developed for silencing malignancy associated genes. Nano drugs fit these needs by targeting tumor and delivering antisense oligonucleotides for silencing of genes. As drugs for the treatment are often administered repeatedly, absence of toxicity and negligible immune response are desirable. In the example presented here, a nano medicine is synthesized from the biodegradable, non-toxic and non-immunogenic platform polymalic acid by controlled chemical ligation of antisense oligonucleotides and tumor targeting molecules. The synthesis and treatment is exemplified for human Her2-positive breast cancer using an experimental mouse model. The case can be translated towards synthesis and treatment of other tumors.
Chemistry, Issue 88, Cancer treatment, personalized medicine, polymalic acid, nanodrug, biopolymer, targeting, host compatibility, biodegradability
Aseptic Laboratory Techniques: Plating Methods
Institutions: University of California, Los Angeles .
Microorganisms are present on all inanimate surfaces creating ubiquitous sources of possible contamination in the laboratory. Experimental success relies on the ability of a scientist to sterilize work surfaces and equipment as well as prevent contact of sterile instruments and solutions with non-sterile surfaces. Here we present the steps for several plating methods routinely used in the laboratory to isolate, propagate, or enumerate microorganisms such as bacteria and phage. All five methods incorporate aseptic technique, or procedures that maintain the sterility of experimental materials. Procedures described include (1) streak-plating bacterial cultures to isolate single colonies, (2) pour-plating and (3) spread-plating to enumerate viable bacterial colonies, (4) soft agar overlays to isolate phage and enumerate plaques, and (5) replica-plating to transfer cells from one plate to another in an identical spatial pattern. These procedures can be performed at the laboratory bench, provided they involve non-pathogenic strains of microorganisms (Biosafety Level 1, BSL-1). If working with BSL-2 organisms, then these manipulations must take place in a biosafety cabinet. Consult the most current edition of the Biosafety in Microbiological and Biomedical Laboratories
(BMBL) as well as Material Safety Data Sheets
(MSDS) for Infectious Substances to determine the biohazard classification as well as the safety precautions and containment facilities required for the microorganism in question. Bacterial strains and phage stocks can be obtained from research investigators, companies, and collections maintained by particular organizations such as the American Type Culture Collection
(ATCC). It is recommended that non-pathogenic strains be used when learning the various plating methods. By following the procedures described in this protocol, students should be able to:
● Perform plating procedures without contaminating media.
● Isolate single bacterial colonies by the streak-plating method.
● Use pour-plating and spread-plating methods to determine the concentration of bacteria.
● Perform soft agar overlays when working with phage.
● Transfer bacterial cells from one plate to another using the replica-plating procedure.
● Given an experimental task, select the appropriate plating method.
Basic Protocols, Issue 63, Streak plates, pour plates, soft agar overlays, spread plates, replica plates, bacteria, colonies, phage, plaques, dilutions
Testing Nicotine Tolerance in Aphids Using an Artificial Diet Experiment
Institutions: Cornell University.
Plants may upregulate the production of many different seconday metabolites in response to insect feeding. One of these metabolites, nicotine, is well know to have insecticidal properties. One response of tobacco plants to herbivory, or being gnawed upon by insects, is to increase the production of this neurotoxic alkaloid. Here, we will demonstrate how to set up an experiment to address this question of whether a tobacco-adapted strain of the green peach aphid, Myzus persicae, can tolerate higher levels of nicotine than the a strain of this insect that does not infest tobacco in the field.
Plant Biology, Issue 15, Annual Review, Nicotine, Aphids, Plant Feeding Resistance, Tobacco
Biology of Microbial Communities - Interview
Institutions: Harvard Medical School.
Microbiology, issue 4, microbial community, DNA, extraction, gut, termit
Studies of Bacterial Chemotaxis Using Microfluidics - Interview
Institutions: MIT - Massachusetts Institute of Technology.
Microbiology, issue 4, microbial community, chemotaxis, microfluidics
Microbial Communities in Nature and Laboratory - Interview
Institutions: MIT - Massachusetts Institute of Technology.
Microbiology, issue 4, microbial community, biofilm, genome
A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems
Institutions: Helmholtz Centre for Environmental Research - UFZ, Helmholtz Centre for Environmental Research - UFZ.
Bioavailability of contaminants is a prerequisite for their effective biodegradation in soil. The average bulk concentration of a contaminant, however, is not an appropriate measure for its availability; bioavailability rather depends on the dynamic interplay of potential mass transfer (flux) of a compound to a microbial cell and the capacity of the latter to degrade the compound. In water-unsaturated parts of the soil, mycelia have been shown to overcome bioavailability limitations by actively transporting and mobilizing organic compounds over the range of centimeters. Whereas the extent of mycelia-based transport can be quantified easily by chemical means, verification of the contaminant-bioavailability to bacterial cells requires a biological method. Addressing this constraint, we chose the PAH fluorene (FLU) as a model compound and developed a water unsaturated model microcosm linking a spatially separated FLU point source and the FLU degrading bioreporter bacterium Burkholderia sartisoli
RP037-mChe by a mycelial network of Pythium ultimum
. Since the bioreporter expresses eGFP in response of the PAH flux to the cell, bacterial FLU exposure and degradation could be monitored directly in the microcosms via confocal laser scanning microscopy (CLSM). CLSM and image analyses revealed a significant increase of the eGFP expression in the presence of P. ultimum
compared to controls without mycelia or FLU thus indicating FLU bioavailability to bacteria after mycelia-mediated transport. CLSM results were supported by chemical analyses in identical microcosms. The developed microcosm proved suitable to investigate contaminant bioavailability and to concomitantly visualize the involved bacteria-mycelial interactions.
Environmental Sciences, Issue 94, PAH, bioavailability, mycelia, translocation, volatility, bioreporter, CLSM, biodegradation, fluorene

domain: biology


Sahu, Sujit K. and Roberts, Gareth O.
On convergence of the EM algorithm and the Gibbs sampler
Statistics and Computing, 9, (1), . (doi:10.1023/A:1008814227332).
Full text not available from this repository.
In this article we investigate the relationship between the EM algorithm and the Gibbs sampler. We show that the approximate rate of convergence of the Gibbs sampler by Gaussian approximation is equal to that of the corresponding EM-type algorithm. This helps in implementing either of the algorithms as improvement strategies for one algorithm can be directly transported to the other. In particular, by running the EM algorithm we know approximately how many iterations are needed for convergence of the Gibbs sampler. We also obtain a result that under certain conditions, the EM algorithm used for finding the maximum likelihood estimates can be slower to converge than the corresponding Gibbs sampler for Bayesian inference. We illustrate our results in a number of realistic examples all based on the generalized linear mixed models.
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Fighting for our freedom, being independent, fighting against someone controlling us we have a whole history of that,” she said, describing victim blaming as an attempt to quiet the notion that things are not always in our control..
The guy who’s eating the steak is sluggish in the fourth quarter. Advertising Age noted the channel had 175,000 viewers in the same slot a year ago.
Click for full scheduleStoried football Hall of Famer Joe Montana tells NBC’s Eric Hinton that Colin Kaepernick is most likely out of the NFL because of poor play, not his controversial stance of kneeling before the National Anthem last season.
Atlanta led 17 7 after Freeman’s second TD run from 2 yards out and Matt Bryant’s 51 yard field goal. These games will take place on 22 and 29 October, again with specific dates yet to be decided..
Before he left the stage, McKinley reverted to the kind of emotion he regularly displayed on the field while collecting 10 sacks and 18 tackles for loss last season, emphatically referencing his grandmother before the microphone was even thrust in front of him..
Play like that for 60 minutes Friday against fifth ranked USC and WSU will be 5 0.. Today’s settlement involves only the NFL.
One brief filed in New York pointed to a close Nov. Some may be leaving for different reasons, but each illustrates a nascent rebellion against an elemental part of the game to never, ever quit..
So I think that wore on him a little bit personally, but hes never been anyone whos going to show it, because hes Hakuna Matata..
Lewis: Praying. Your Connecticut teachers’ pension is in a crisis and almost no one knows it or isn’t willing to talk about it.
It all came apart in nba basketball tops the 4th quarter. Michaud said despite efforts by public health officials to educate the public that vaccines are overwhelmingly safe and effective, there still a lot of fear about vaccines.
NFL spokesman Joe Lockhart called the president statements believe there was a statement our owners are afraid of our players and owners requested intervention by political leaders to kick this off, Lockhart said.
“It’s unfortunate that the President decided to use his immense platform to make divisive and offensive statements about our players and the NFL,” Packers President and CEO Mark Murphy said in a statement released Saturday.
For a league filled with physical specimens, it’s a landmark achievement, regardless of the circumstance. Xbox LiveUsing the Ethernet cable port, gamers could connect to the Internet through the Xbox Live system and compete with millions of other Xbox players worldwide.
It’s also a world of “bros”: five brothers, all big and athletic and competitive as hell. With Eddie Johnson and Obafemi Martins already in the fold, wouldn’t Dempsey’s $5 million salary (and $368K cap hit) be more wisely spread among a passel of reinforcements for the Sounders’ aging defense? You can at least make the argument.
Can find words that appropriately express how heartbroken I am to see the constant smears against Colin, a person who helped start the movement with only the very best of intentions, Reid went on to write.
I was reckless. Starting Thursday, Bud Light is hoping to fill that bill with beer glasses that light up after touchdowns.. If the Boston Red Sox are favored over the New York Yankees, the moneyline might be 130 for the Red Sox and +110 for the Yankees.
“I thought our kickers were terrific. The protest sparked a debate in terms of whether Kaepernick’s action was disrespectful or a form of freedom of speech.
If women are at the party, a battle of the sexes trivia game can be fun to play at work as well. Peterson was released in the off season after 10 years as a Viking..
It began with the president calling for NFL protesters to be fired and continued Saturday when he rescinded a White House invitation for the NBA champion Golden State Warriors over star Stephen Curry criticism..
Most hitting and physical contact in football happens on the line of scrimmage. Superficial beauty catches our attention but natural beauty keeps it.” Thomas Edwards Jr., 26, Founder of The Professional WingmanDrop Hints to Avoid Disappointing Gifts, Please!”When it comes to gift giving, I wish women knew that we are about as clueless as a five year old boy doing quantum physics.
They receive negative points for yardage and custom baseball jerseys touchdowns against them.. W nearly came back from deficits of classic sports jerseys 21 points against Stony Brook and 20 nhl jerseys 2016 against Elon,.
But I like where we’re at as a oakley sunglasses for men group. Kaepernick’s decision to kneel during the national anthem last season, which he said was to protest racial injustice, has gained public support in recent weeks from NFL stars like Aaron Rodgers and Cam Newton.


“We are all faced with a series of opportunities brilliantly disguised as impossible situations.”- Charles R. Swindoll
I came across today’s quote a while back, but I’m finding it very pertinent to my current situation. Not long ago I picked my move in day to make the big trek from the East to the West and as that day was Wednesday September 2nd, I had my room packed and my plane tickets a-waitin’ when I received a call Tuesday September 1st to learn that my home-to-be was in the path of the California fires. My landlady informed me that our neighbors had been evacuated and that I should look into temporary housing somewhere a little safer. She told me that if there was anyway to delay my flight it would probably be for the best.
Well, as luck or fate would have it, my family friends living near San Diego graciously opened their home to me so that I wouldn’t have to change my plans because of some pesky fire. I’ve had a wonderful time catching up and helping around the house where I can and it’s because of their generosity that I was able to attend the voice over mixer in LA last night hosted by Stephanie Ciccarelli and Voices.com
I had the opportunity to connect with my friend Andrea Christensen and her voice coach and friend Julie Williams. Julie runs a wonderful voice over magazine at www.voice-overs.com. It is an invaluable resource and I highly recommend subscribing to it. I had the chance to speak briefly with Stephanie Ciccarelli about her blog at voices.com and to thank her for organizing such a great event. It was an amazing evening, and I’m so grateful to have had the opportunity to meet with so many talented people. The drive from San Diego county and back was totally worth it!
Thank you to everyone who shared their time with me last night, I had a wonderful time and I look forward to meeting again. Until then, take care!
All the best,


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Father's Day is in a week, are you ready?
Just like any other special occasions, adding unique and personalized wrapping for your gift can make the event more special and memorable.
In the past, the shirt style wrapping has always been one of the most popular styles for my customers, students and the audience of my YouTube videos. It's a stylish, unique and fun way to celebrate Father's Day.
What type of decorations do you add to your gift wrapping?
There are so many ways to upgrade your gift wrapping by adding ornamentations to the package, and one of them is creating ribbon arts.
So today's video is all about adding various themed ribbon arts to your package using my recent favorite, cotton 100% paper ribbon from CreamCityRibbon.com.
Have you added pleats designs to your gift wrapping?
It's a fun and easy way to upgrade your gift wrapping but making a perfect pleats design is not always easy for everyone, especially for the beginners. I've received requests from some of you to make a lesson video on how to fold paper straight and create pleats equally in the same size.
If you love DIY craft projects, the holiday season must be a fun and exciting time for you! Decorating trees and rooms, making your own holiday Christmas wreath and ornaments, designing and wrapping your gifts, making handmade holiday cards...there are so many creative projects to do.
A book is one of the most items I give and also receive as a gift. I'm sure it's the same way to many of you as we know that good books often give us new skills & knowledge, entertainment & enjoyment, or happiness & satisfaction!
Are you planning to host a holiday party? or perhaps giving thank you gifts to friends and co-workers this holiday season?
Here is an easy way to create DIY custom gift bag!

domain: hobby


This mod adds electric trains which require power delivered by overhead lines.
This fork of Realistic Electric Trains is an attempt to balance it with the features of Krastorio 2
Performant, semi-realistic power distribution overhaul.
Power poles can explode if the total electrical consumption on the network is too high. Use transformers to separate networks so that subnetworks do not take more power than they can handle.


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Question by RedditorNomad in 14/11/2023 - 7 Answer(s) - 26 Vote(s)
Category: Mental Health and Wellness
Managing Workplace Stress: Strategies for a Healthier Work Environment
How can I cope with stress at work and create a mentally healthier work environment? What strategies can help reduce workplace stress?
Answer #1 by GoldRushRebel in 01/12/2023 - 4 Vote(s)
During a challenging period, mentorship played a pivotal role. Having a mentor provided invaluable guidance and insights, offering a roadmap to overcome obstacles and move forward in my career.
Answer #2 by ContentCuratorQueen in 30/11/2023 - 8 Vote(s)
Crisis management in my career involved proactive planning. Anticipating potential challenges and having contingency plans in place provided a sense of control during uncertain times.
Answer #3 by UpvotePrincess2 in 28/11/2023 - 18 Vote(s)
Acknowledging and learning from failures was transformative. Rather than viewing setbacks as defeats, I treated them as stepping stones toward success, fostering a resilient mindset in my career.
Answer #4 by UpvotePrincess in 27/11/2023 - 17 Vote(s)
Navigating career setbacks has been a journey for me. I discovered that embracing setbacks as opportunities for learning and growth has been crucial to my professional development.
Answer #5 by UpvoteKing in 26/11/2023 - 22 Vote(s)
Negotiating a job offer after a setback required highlighting my strengths. I emphasized how my experiences, though challenging, equipped me with unique skills that would contribute positively to the role.
Answer #6 by DownvoteDuchess in 23/11/2023 - 30 Vote(s)
Resilience, for me, was about staying committed to my goals despite obstacles. It meant adapting my strategy, honing new skills, and ultimately turning setbacks into stepping stones for future success.
Nurturing Mental Well-being: Strategies to Cope with Workplace Stress
Introduction:In the fast-paced and demanding world of work, stress has become an almost ubiquitous companion for many professionals. The impact of workplace stress on mental health is substantial, leading to burnout, reduced productivity, and a decline in overall well-being. This article explores effective strategies to cope with stress at work and create a mentally healthier work environment, drawing insights from experts in psychology and workplace well-being.
1. Recognizing the Signs of Stress:Before implementing strategies to cope with workplace stress, it's crucial to recognize the signs and symptoms. Dr. Elizabeth Scott, a wellness coach and author, emphasizes the importance of self-awareness in identifying stressors. Common signs include changes in sleep patterns, increased irritability, physical symptoms like headaches, and a persistent sense of overwhelm. Recognizing these signs is the first step in addressing stress effectively.
2. Establishing Healthy Work Boundaries:Setting clear boundaries between work and personal life is essential for managing stress. Author and productivity expert Cal Newport, in his book "Deep Work," emphasizes the importance of creating intentional and focused work periods. Establish specific working hours and avoid the temptation to continuously check emails or engage in work-related tasks during personal time. Boundaries create a sense of control and prevent work-related stress from permeating personal life.
3. Practicing Mindfulness and Relaxation Techniques:Mindfulness and relaxation techniques have proven effective in reducing stress and promoting mental well-being. In "The Relaxation and Stress Reduction Workbook," authors Martha Davis, Elizabeth Robbins Eshelman, and Matthew McKay provide practical exercises to manage stress. Incorporating practices such as deep breathing, meditation, or yoga into the daily routine can help individuals stay grounded and manage stress more effectively.
4. Effective Time Management:Poor time management is a significant contributor to workplace stress. Renowned time management expert Brian Tracy, in his book "Eat That Frog!," advocates for prioritizing tasks and focusing on high-value activities. Create a daily schedule, prioritize tasks, and break down larger projects into manageable steps. This not only enhances productivity but also reduces the feeling of being overwhelmed by a mountain of work.
5. Building a Supportive Work Environment:The workplace itself plays a crucial role in contributing to or mitigating stress. Author and workplace culture expert Patrick Lencioni, in "The Five Dysfunctions of a Team," highlights the importance of a cohesive and supportive team environment. Foster open communication, encourage collaboration, and promote a culture where colleagues support each other. A positive work environment can act as a buffer against stressors.
6. Seeking Social Support:Connecting with colleagues on a personal level and seeking social support is a powerful stress management strategy. In "Daring Greatly," Brené Brown explores the significance of vulnerability and connection. Share concerns with trusted colleagues, build a supportive network, and consider participating in workplace social activities. Having a strong support system can provide a sense of camaraderie and alleviate feelings of isolation.
7. Regular Physical Activity:Exercise has well-documented benefits for mental health and stress reduction. In "Spark: The Revolutionary New Science of Exercise and the Brain," Dr. John J. Ratey explores the connection between physical activity and cognitive well-being. Incorporate regular exercise into your routine, whether it's a brisk walk during lunch or a workout session before or after work. Physical activity releases endorphins, which act as natural stress relievers.
8. Promoting Work-Life Balance:Work-life balance is a critical component of mental well-being. In "Thrive," Arianna Huffington emphasizes the importance of prioritizing well-being over a relentless pursuit of success. Set realistic expectations for workload, take breaks when needed, and use vacation time to recharge. A balanced approach to work and personal life contributes to overall resilience in the face of workplace stress.
9. Providing Training on Stress Management:Employers can play a significant role in reducing workplace stress by offering training programs on stress management. Dr. Cary Cooper, a professor of organizational psychology and health, emphasizes the importance of organizational support in managing stress. Provide employees with resources and training that equip them with effective stress management techniques, fostering a culture that prioritizes mental well-being.
10. Encouraging Regular Breaks and Downtime:Continuous work without breaks can lead to burnout and increased stress levels. In "The Power of Full Engagement," authors Jim Loehr and Tony Schwartz advocate for the importance of managing energy, not time. Encourage employees to take regular breaks during the workday, step away from their desks, and engage in activities that bring joy or relaxation. Scheduled downtime allows for mental recharge and enhances overall resilience to stress.
11. Implementing Flexibility in Work Arrangements:Flexible work arrangements, such as remote work or flexible hours, can contribute significantly to reducing workplace stress. In "Drive: The Surprising Truth About What Motivates Us," Daniel H. Pink explores autonomy as a key motivator. Providing employees with flexibility in how, when, and where they work enhances a sense of control and autonomy, contributing to reduced stress levels.
12. Offering Employee Assistance Programs (EAPs):Employee Assistance Programs (EAPs) provide confidential counseling and support services to employees facing personal or work-related challenges. In "Strategic Human Resource Management," author Catherine Truss discusses the role of support mechanisms in enhancing employee well-being. Employers can implement EAPs to offer professional guidance and resources for employees dealing with stress.
13. Promoting a Healthy Work-Life Integration:Rather than strictly separating work and personal life, consider promoting a healthy work-life integration. In "The 4-Hour Workweek," Tim Ferriss explores the concept of lifestyle design and optimizing work and personal activities. Encourage employees to integrate work tasks with personal activities when possible, fostering a more holistic approach to managing responsibilities and reducing stress.
14. Encouraging Regular Check-Ins and Feedback:Regular check-ins and constructive feedback can play a crucial role in managing workplace stress. In "Radical Candor," author Kim Scott emphasizes the importance of candid communication between supervisors and team members. Regular feedback sessions provide an opportunity to address concerns, clarify expectations, and create a supportive environment for professional growth.
15. Providing Mental Health Resources:Employers should prioritize mental health resources as part of their benefits package. Dr. David Satcher, former U.S. Surgeon General, emphasizes the need to destigmatize mental health issues. Offering access to counseling services, mental health days, and resources that promote mental well-being communicates a commitment to supporting employees' holistic health.
Conclusion:Effectively coping with stress at work and creating a mentally healthier work environment requires a multifaceted approach. By recognizing signs of stress, establishing healthy boundaries, and implementing strategies such as mindfulness, individuals can proactively manage their well-being. Employers play a crucial role in fostering a supportive work environment through training programs, flexibility, and mental health resources. Ultimately, the combination of individual and organizational efforts contributes to a workplace culture that prioritizes mental well-being and helps individuals thrive amidst the challenges of a demanding professional landscape.
- Career Development
- Career Shift and Transition
- Education and Training for Career
- Entrepreneurship and Passive Income
- Freelancing and Gig Economy
- General Career Advice
- Internships and Co-op Programs
- Interview Techniques
- Job Market Trends
- Job Search Strategies
- Leadership and Management
- Mental Health and Wellness
- Networking and LinkedIn
- Personal Branding
- Professional Certifications
- Remote Work and Telecommuting
- Resume and Cover Letter Tips
- Salary Negotiation
- Work Abroad and International Careers
- Work-Life Balance
- Workplace Diversity and Inclusion
- Workplace Issues and Ethics

domain: psychology


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With global travel restrictions, we are arranging online medical consultations with best medical centers as well as pre-bookings for future medical treatments. Explore Now… Go Later. Continue your healthcare journey.
Rejuve is a top-notch plastic surgery clinic by Dr. Edgar Torres in Cancun, Mexico. They provide the best aesthetic procedures for face and body using the most advanced techniques and at affordable prices. The key procedures include Facelift, Neck Lift, Blepharoplasty, Brow Lift, Liposculpture, Abdominoplasty, Breast Augmentation and Brazilian Butt Lift.
Nirunda International Aesthetic Clinic is the best aesthetic in Bangkok, Thailand. International Board Certified Plastic Surgeons Team and The best of skin care and anti aging treatments is available at our clinic. See what our innovative technologies can do for you!
Teknon Medical Center located in Barcelona, Spain provides comprehensive medical services and covers all medical and surgical specialties, including Oncology, Cardiology and Cardiovascular Surgery, Traumatology, Aesthetic/Plastic Surgery, and Neurosurgery. Learn more about this leading European medical center.
Quironsalud University Hospital Madrid is a multi-specialty hospital in Madrid, Spain. Ideal for international patients, the hospital a wide range of effective and affordable treatments such as cardiac surgery, oncology, plastic surgery, neurosurgery, robotic surgery, general surgery, urology, fertility treatments and much more.
Pectoral augmentation or Pectoral reshaping packages are highly popular in New Delhi, India. The medical tourism destination is most known for expert cosmetologists offering suitable packages to boost men’s confidence and good looks.
Mexicali, Mexico is a destination renowned for its cosmetic and plastic medical centers, dedicated medical team and affordable prices for flawless medical care. Therefore, numerous patients from all over the world choose to undergo the Tummy Tuck with Liposuction and Brazilian Butt Lift surgery here.
Are you tired of fighting those stubborn fat deposits on your body? Do you have a healthy diet and exercise regularly but that bulge just won't go away?
Then you may have to consider having liposuction, one of the most popular and common plastic surgeries in the world in one of the most exotic places in the world, Manila, Philippines!
Are you looking for the best facelift surgery package? Izmir is a top destination in Turkey for patients from all over the world who are looking for flawless and affordable facelift procedures. Click here for the best facelift package in Izmir.

domain: medical


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As reported by SF Gate and other media outlets, Governor Brown has signed Senate Bill 1413, allowing school districts in high-priced cities to build teacher-only affordable housing on district property.
According to Redfin, a national real estate brokerage, of California’s 31 most-populous counties, only 17 percent of homes for sale in those counties were affordable on the state’s average teacher salary of $73,536. “Affordable” means the monthly mortgage payment would eat up less than 30 percent of a person’s gross monthly salary. California’s economic boom has not only affected key metro areas but has created a ripple effect across entire regions, resulting in a serious recruitment and retention challenge for schools across the state – and a quality of life issue for many teachers and school employees.
This bill will now authorize a school district to establish and implement programs, especially land leases, that address the housing needs of teachers and school district employees who face challenges in securing affordable housing. Equally important, the law permits school districts and developers in receipt of local or state funds or tax credits designated for affordable rental housing to restrict occupancy to teachers and school district employees on land owned by school districts, so long as that housing does not violate any other applicable laws.
SB 1413 has significant implications for cities and school districts seeking creative options to address the housing affordability crisis. For the past 12 years, DCG has worked with K-14 schools across the state to evaluate the most strategic use of their real property, and is offering a complimentary briefing to help leaders understand the implications and opportunities for their district.

domain: sociology


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From new movies and live concerts to cricket matches, people can now stream almost everything in seconds, in the comfort of their homes, through a single click. JioCinema, the official digital streaming partner of IPL 2023, clocked over 1300 crore views in the first five weeks, making it an instant hit.
In fact, they love this application a little too much as a cricket fan was seen streaming the live action on his phone, at the stadium where the match was being played right in front of his eyes!
Gabbar, a parody account, posted the video on their social media handle where the camera starts with a match being played in the cricket stadium ground.
Later, the camera gradually pans towards a man, laying on his back in the seating area, and watching the same match on his phone!
The 15-second video, which has garnered over 750.5K views, went viral in no time and here’s what other fans think of this.
Now, that’s how we take ‘sound quality’ to another level!

domain: news


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Advances in LC Particle Technology: The Truth and Misconception of pH Stability
14 Jan 2019
Using a broad range of mobile-phase pH to manipulate compound retention, loading, and selectivity greatly benefits separations scientists. There are many considerations that prevent scientists from reaching their separation objectives that include choice of particle and stationary phase, understanding the benefits mobile-phase buffer selection, and dispelling myths and misconceptions that are associated with using an extended pH range for method development.
During this presentation you will learn about recent developments in particle technology that will help you to develop reliable and robust methods.
This introductory presentation provides an update towards the latest advances in LC column sorbents, and covers hybrid and charged-surface hybrid-based sorbents for LC method development.
In this presentation you will learn how to:
- Develop methods using high pH mobile-phases
- Choose the most appropriate conditions based compound properties
- Benefit from using extended mobile phase pH to increase compound retention, sample loading and separation selectivity

domain: chemistry


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WATCH – Massive alligator may be world’s largest Must see video of worlds largest alligator
If you live in the US state of Florida, you live in a world full of alligators. The large reptiles are known to reside at the Circle B Bar Reserve nature preserve near Lakeland. That’s where Facebook user Kim Joiner encountered a particularly gigantic representative of the species when it sauntered across her path on Sunday. Her video of the event is now a Facebook hit.


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Have you ever thought your coffee tasted better when drinking out of a nice mug at a quaint coffee shop?
Well, your taste buds are not deceiving you. There are reasons that drinking from cups made of different materials will affect how your coffee tastes. And it’s not just coffee. All the utensils, dinnerware, and containers can affect the taste of the food and drink you are consuming. All materials react with each other and can change the flavor of what you are putting inside your body.
Taste is just one factor that can be affected by what your mug is made out of and it will be what we are focusing on today. Taste can be affected by a lot of things. You may be making your coffee bland and flavorless without even knowing.
Today, we will be learning why your coffee tastes different depending on your cup and which material is the best for your favorite brew.
Why coffee tastes better in a ceramic cup
There have been studies done that show that your eyes and how your food looks actually play a part in how it tastes. This is why when you go out to a fancy restaurant it may taste better than normal food that is carelessly plopped onto your plate.
A carefully arranged and organized portion represents in your brain a higher quality and tastier meal, and as a result, you actually think it tastes better. This is the same a drinking coffee out of a proper ceramic mug.
It is part of human nature to perceive things based on all of our senses, not just the taste. This is why the coffee tastes better in a ceramic cup as opposed to a paper cup. The weight of the cup and the texture can also play a part in getting you in the state of mind to enjoy your coffee just a bit more.
The coffee will also be insulated more in a ceramic cup. A paper or stainless steel cup does not have any insulating properties and can cause your coffee to rapidly cool off changing the flavor of the coffee.
Believe it or not, the color of your cup also plays a part in how your coffee tastes. Ceramic cups allow for more expressive designs and beautiful glazes which play a part in how your coffee tastes.
The coffee journal Flavour did research that showed people thought that coffee in white mugs would taste sweeter. They also reported that tasters thought there were significant differences in the taste of the same coffee when served in blue, clear, and white mugs.
How cup shapes can affect the taste of your coffee
A study published about Food Quality and Preference showed that people perceived coffee to be sweeter when presented in a larger and deeper mug. This is probably because these kinds of mugs are usually served with milky lattes or specialty drinks.
When given smaller cups people usually associate them with darker and more bitter drinks with less milk. Espresso and other espresso-based drinks can come to mind when being served coffee in a smaller cup.
Another interesting remark from the 300 participants that attended the study was, a majority of the participants would expect to pay more when given one of the larger mugs.
We also pinpointed the two shapes of a mug that can affect how people taste their coffee. It is at the headspace, the area between the lip of the mug and the coffee, and the circumference of the cup.
The headspace can affect the taste of the coffee because this space between the coffee and the top of the cup allows the coffee to aerate when you take a sip of your coffee. This causes you to smell all the nice coffee aromas which is a large part of the taste.
The circumference of the cup also matters because it allows your nose to be closer to the coffee further improving the scent of the coffee. Studies have shown that the smell of coffee is one of the largest factors to how much we enjoy it.
The taste of coffee at different temperatures
According to Barry Green, a taste perception scientist at Yale University, hot coffee releases more aromatic compounds so like we stated before it will impact the taste of your coffee more. If you’ve ever smelled room temperature or cold coffee, you would know that it does not compare to a freshly brewed pot of coffee.
Ceramic mugs keep your drinks hot for longer and as a result, keeps the delicate aromas and flavor notes in your coffee.
Once your coffee starts getting cold there are actually chemical changes that happen that can change the way the coffee tastes.
Coffee is an extremely complex and sophisticated drink comprised of over 1000 aroma compounds that we can smell and taste, even if we are not consciously aware of it. A lot of the compounds are released when there is heat as volatile compounds which are turned into gasses and slowly evaporate over time.
Over time all these compounds are released and when the coffee is cold we are usually left with a more bland version of what we started with.
Acidity is also perceived best at lower temperatures. This allows you to taste more acidity and can even bring out flavors that you would normally not be able to taste in a hot cup of coffee. There are some Kenyan coffees that become more vibrant with these higher acidity levels.
Materials you should avoid when drinking coffee
As stated before, ceramic coffee cups are the best type of cups to drink coffee out of. Here are a few different types of cups and why you should probably avoid them.
Plastic would seem like a good choice because of its lack of distinct taste. However, due to coffee being a warm liquid, it can affect the plastic over time and leech chemicals into your coffee if you do not have a safe container.
Plastics can also absorb odors, flavors, and oils. This can probably affect the taste of coffee if you use the same container over a long period of time.
We’ve all had the office coffee out of a paper cup and know how bad these cups can be. Paper is probably the worst kind of cup to drink coffee out of because they are usually not made for hot drinks and because paper can impart a flavor into your coffee.
Sometimes, these paper cups can have a waxy residue and coating on the inside of the cup and form a thin film on top of your coffee. No thank you!
When paper cups are heated they can start to smell like cardboard as well, this will certainly disrupt your olfactory system and make your coffee seem a lot worse than it probably is.
Last but not least paper cups are very wasteful, if you reuse a ceramic cup you will leave a smaller carbon footprint, and is a more sustainable way to drink your coffee.
Stainless steel-like plastic can impart flavors into your coffee. Sometimes you can get a metallic taste from the container.
Stainless steel also acts as a heatsink and draws all the heat out of your coffee and into your container. Your container will be scalding hot, but your coffee will get cold extremely quickly if you do not have a double-wall insulated stainless steel container!
Glass is probably the next best choice next to a ceramic coffee cup. It has almost all the same benefits as ceramic, but it does not look great or artistic. Glass also does not hold heat as well as ceramic does, but can still do the job. The neutral taste and small carbon footprint make glass a great alternative to ceramic coffee cups.

domain: food


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Meet the Chefs
If the old adage is true;
the quickest way to a man’s heart is through his stomach, then the one thing our clientele may savor as much as the entertainment is the food….sorry dancers! Thanks to our talented team of culinary professionals, The Men’s Club cuisine rivals fine-dining restaurants and top-notch steakhouses alike – and inspires almost as much passion for the plate as for the performers.
Keith Roybal graduated with honors from the New York Restaurant School, working an apprenticeship at a four-star restaurant on Manhattan’s Upper East Side. During his career Keith used the skills he honed to create variety menus for other four-star restaurants. Inspired by the art and use of different ingredients, Keith’s favorite things about cooking are being surrounded by fresh food, and the detailed preparation that goes on in the kitchen.
Keith’s advice to aspiring chefs: “Journey into the unknown and don’t look back. Give yourself to the craft.”
Chef Paolo Stoltz began his career-long affair with food as a boy in Italy. Brought up in a family so passionate about cooking and eating, his devotion to the culinary arts was destined to flourish. And it did. Chef Paolo was heavily influenced by his love for Italian food and by the fresh fare that his Grandmother and Mother created in their kitchens. From Italy to California to Texas and many points in between, Chef Paolo has had extensive experience in kitchens around the world.
The Men’s Club fine-dining experience adds something special to every encounter; business meetings, friends out for fun, couples on a date or any celebratory occasion. From the meat lovers “Kick-Ass Filet” and fresh-from-the-tank Live Maine Lobster to amazing All-American burgers, spectacular seafood and salads, delectable pasta dishes and so much more, our Chefs at The Men’s Club get a five-star rating from all who savor the flavors they bring to the table.


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This holiday season, teddy bear shop manager Sasha Silverman must solve the slaying of Santa Bear . . .
Sasha and her sister Maddie are thrilled that the Silver Bear Shop and Factory has won the Teddy Bear Keepsake Contest, which means they get to produce a holiday specialty toy, a wizard bear named “Beary Potter.” Promising to be just as magical is Silver Hollow's annual tree-lighting ceremony and village parade. Only one hitch: the parade's mascot, Santa Bear—played by Mayor Cal Bloom—is missing.
After a frantic search among the floats, Bloom is found dead. When the outfit is removed, it's clear the mayor's been electrocuted. Who zapped hizzoner and then stuffed him into his Santa Bear suit? While the police investigate the grisly crime, Sasha attempts to track down the murderer herself, with some help from the Guilty Pleasures Gossip Club. Can they wrap up this case in time for Christmas—or will Sasha meet her own shocking end?
Praise for BEARLY DEPARTED
“You'll fall in love with this delightful debut mystery.”
—Victoria Thompson, bestselling author of Murder in Morningside Heights
“The first in a new series features a complex plot awash in red herrings, a perky heroine . . . and everything you ever wanted to know about teddy bears.”
“The appealing, impulsive amateur sleuth, dedicated to the family business, will appeal to fans of character-driven cozies.”
“Entertaining . . . inhabited by quirky, fully developed characters and good dogs and cats.”
by Meg Macy
by Arthur Conan Doyle
by John J. Lamb
by Agatha Christie
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domain: literature


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Everything is Connected
Mar 28, 2017 09:43PM
Nicholas J. Meyer, DDS, of Millennium Dental Associates, in Scottsdale, has published a new book, The Holistic Dental Matrix: How Your Teeth Control Your Health and Well-Being.
He explains that the Holistic Dental Matrix is a fascinating connection between overall health, chronic disease states and dental health. Meyer has discovered that we can’t just treat the symptoms of our dental issues; we have to follow the symptoms in order to find the real problem.
Meyer graduated from Loyola University, Chicago College of Dental Surgery, International College of Craniomandibular Orthopedics, is a diplomate of the American Academy of Pain Management, Academy of Clinical Sleep Disorders Discipline, and is a certified biological dentist and a doctor of natural medicine in the Pastoral Medical Association.
Special book signing and reception at 6 p.m. Apr. 6, at The Orchard in Phoenix (RSVP requested at 602-573-7496). Purchase the book at Amazon.com or DrNicholasMeyer.com. For more information, visit MillDental.com.


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* Augusta Industries reports record revenue for Q2 of 2016
* H1 earnings per share $0.001 Source text for Eikon: Further company coverage:
All quotes delayed a minimum of 15 minutes. See here for a complete list of exchanges and delays.
© 2018 Reuters. All Rights Reserved.

domain: economics


text: The evolution of shear bands (SBs) into cracks was observed by using a high-resolution scanning electron microscope in Zr59Cu20Al10Ni8Ti3 metallic glassy samples after a small punch test with different strain rates. As shear strain increased along a radial SB, three distinctive regions of morphologies were found (I) bonded SB, (II) microcrack plus bonded SB, and (III) full crack. In region II with moderate shear strain, some glassy “extrusions” were also observed. Once shear offset increases to a critical value, the SB becomes a full crack. For two different SBs in one specimen, the critical shear offsets maintain approximately the same value, which sheds light on the critical shear failure condition of metallic glass. The critical shear offset was also found to be sensitive to the strain rate and a higher strain rate led to less critical shear offset. It is suggested that the structure evolution and heat evolution within a shearing SB should be responsible for the previous results.


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Where is duty? Where is sacrifice? It’s trampled under your pretty foot again.[to Rhaenyra]
How sweetly the fox speaks when it’s been cornered by the hounds.
A true queen counts the cost to her people.
We do not rule but we may guide the men that do.[to Rhaenys]


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Well, the shipment of assorted flours and unusual tools finally made its way across the country. I decided not to wait for the weekend to start baking. I knew if I hurried home from work and started the dough I would have enough time to finish. The dough was straight forward. You make two batches of dough that differ only in their coloring. I bought the caramel coloring from KA. It is an incredibly dark, cornstarch like powder. It has a bitter taste straight from the container, but I couldn't taste it in the loaf. I used two tablespoons.
Once the dough has fermented, I used the alternating layers method from the BBA book to form the bulls eyes. I have two large loaf pans that were perfect for the loaves. They rose to a nice shape, baked up in the oven. They were almost finished. We were force to go to bed without sampling the finished product.
My main stakeholder is enjoying this rye bread. We reviewed the breads up to this point and although we could find a few that we won't be baking again, we cannot determine a clear winner. The multigrain extraordinaire, the foccacio, the italian bread are in the running for favorite. My husband would add this rye bread to the list.


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What You Need to Know About Social Security
Every so often, you’ll hear about Social Security benefits running out. But is there truth to the fears, or is it all hype?
Have A Question About This Topic?
For women, retirement strategy is a long race. It’s helpful to know the route.
Learn a little about worldwide currency in this fun infographic
Individuals have three basic choices with the 401(k) account they accrued at a previous employer.


Wednesday Mar 5
Sierra Club to celebrate spring by drowning a snowman in effigy
New London - The Sierra Club's Southeast Group will sponsor its annual spring social at 5:30 p.m. March 19 at the Nautilus Room at Ocean Beach Park.
Trending on the Topix Network
Wednesday Mar 5
Call to Artists: Latin Network for the Visual Arts Accepting...
GALES FERRY - The Latin Network for the Visual Arts invites Latin artists to submit artwork to be considered for Latin Views 2014, its 7th and final Biennial Exhibition at the University of Connecticut's Avery Point Campus in Groton at the Alexey von Schlippe Gallery of Art in the historic Branford House Mansion.
Mon Mar 03, 2014
Driver arrested after crashing into pole in Ledyard
Ledyard police charged a 22-year-old man with operating under the influence of drugs and/or alcohol, evading responsibility, failure to drive right, and operating a vehicle without a license following a single-car crash into a utility pole at 4:35 a.m. Monday at Washington Drive and Long Cove Road in Gales Ferry.
Mon Feb 24, 2014
Police logs for Feb. 24, 2014
Erin E. Dunning, 36, of 6 Norman Drive, Gales Ferry, was charged Sunday with driving under the influence of alcohol or drugs.
Sat Feb 22, 2014
May I have some more, please?
With temperatures reaching the mid-40s, many cast aside their winter parkas and instead donned sweaters and light jackets for the 11th Annual Cabin Fever Festival and Chowder Cook-Off.
Ledyard McDonald's restaurant evacuated after car strikes gas meter
The McDonald's Restaurant on Route 12 was evacuated and closed for a time after a vehicle entering the drive-through lane veered into the building and struck the natural gas meter shortly after midnight, Ledyard police and fire officials said.
Thu Feb 20, 2014
Wed Feb 19, 2014
New London sidewalk superhero
Joe Massimino of Gales Ferry, owner of Sarge's Comic and Games on State Street in New London, shovels snow Tuesday off the sidewalk in front of his business.
Tough day to be on the road
Motorists on Interstate 395 north in Montville swerve Tuesday to avoid a car that had spun out in the center median.
Mon Feb 17, 2014
Police: Norwich man steals car, leads police in pursuit through several towns
A Taftville man was charged with motor vehicle theft and reckless driving, among other charges, after he led Norwich and state police in a pursuit through several towns with a stolen vehicle on Sunday night, according to police.
Fri Feb 14, 2014
Two-car garage in Gales Ferry destroyed by fire
A two-car, detached garage at 3 Sleepy Hollow Pentway in Ledyard was destroyed early Thursday morning in a fire.
Thu Feb 13, 2014
Fire destroys garage in Gales Ferry
Gales Ferry Fire Chief Anthony Saccone said the fire was reported at 7:30 a.m., but when they arrived at the scene the garage was completely engulfed in flames.


text:
A fun, friendly place to discuss just about everything.
Lost Message Board, Lost and General Chat about the show and everything else.
A safe place to call home, have debates, chat, discuss the day's events, the political climate and life in general. Decorum is important in my house!
Free forum : GENERAL INFORMATION ABOUT BRADY PRODUCTS THAT MAY NOT BE INCLUDED IN THE CATALOG, PART DESCRIPTION OR SPECIFICATIONS.
General discussion on Advances in Gas Turbine Engine Development.
Questions, lessons learned, and general information related to the District Wide RCS Contract
Native American Spirituality, Vision Quests, Nature Oriented Spirituality, and General Spiritual Support.
Where Defcon Players Come to Relax
A Forum for the use of LHS students only.
PokeCafe is a forum to discuss all things Pokémon! Including the Pokémon Anime, Pokemon Trading Card Game, and anything Pokémon in general! Hope you enjoy!
Free forum : Racing forum. Free forum : Dos Racing. Free forum Dos Racing Racing forum
A place for members to have discussions relevant to the league, baseball in general, or whatever may interest them.
free forum : A general discussion forum for events, politics and issues effecting Dent County and it's citizens.
A general chatting forum about gaming, hobbies and even more.
This is a build form for www.simmons4x4.com. Here you can find builds on each of the vehicles listed on our site along with general discussion areas.
This is where you can store all of your character or characters' ref sheets, any pictures, backstory, and general information about them.
A place for clan members to meet, communicate and discuss items. Also a good place to store regularly shared info and interact in general.
Free forum : a
A place where Pretty Cure fans, and fans of media in general, can congregate and express themselves freely!
Craft beer discussion board and forum. A place to talk about anything relating to craft beer such as news, new arrivals, brewing, festivals, and general discussion.
Welcome to prepthub.com live Q&A! This forum is meant to answer general questions. Any specific questions regarding a program should be directed towards them.
This is a general forum about the anime Pokémon
Dynamic Chat is a general discussion chat forum where you can talk about anything that you like!
An internet community directed at general discussion & topics, humor and randomness.
Racing game discussion, casual and competitive racing and general forum. Predominately on the Playstation platform.
This is the general forum for discussion and chatter for the General Tso Clan on Clash of Clans!
A place to chill and discuss various topics with others. Removed on: 20/01/2016
A public forum for the discussion of airsoft, planning of games, and the general growing of the community within the Lower Mainland.


iPad Air 2 With an integrated memory of 16 GB the Apple iPad Air 2 Wi-Fi + Cellular makes storing user data and downloaded content easy. Running on iOS operating system, this Apple tablet offers a variety of handy features, as well as Wi-Fi + 4G connectivity. Additionally, this Apple iPad Air 2 weighs 0.44 kg (1 lb.) and comes in space grey color with 9.7in (24.64 cm) display, ensuring convenient usage and menu navigation. This device is unlocked and compatible with O2, Three, Vodafone (UK) and EE Carriers.
Refurbished iPad Air 2 Wi-Fi + Cellular (Unlocked) 128GB
Brand Apple Family Line Apple iPad Air 2 9.7" Model Wifi SIM Type Storage Capacity 128GB RAM 2GB Colour Champagne Gold Battery
Operating System iOS Camera Resolution
Network Technology GSM / CDMA / HSPA / EVDO / LTE Battery Life Up to 10 hrs talk time Display Resolution 1536 x 2048 pixels Display Type LED-backlit IPS LCD capacitive touchscreen, 16M colors Diagonal Screen Size 9.7 inch


Designed for durability and performance, the On Stage MBP7000 Handheld Boom Microphone Pole
is a great solution for ENG, EFP and other field recording applications. Our Boom Pole is machined from a sturdy and lightweight aluminum, which allows you to mount your microphone securely and follow your sound source with ease.
The On Stage MBP7000 Handheld Boom Microphone Pole features three telescopic rods that utilize a twist-and-lock system, creating quick, simple and efficient settings extending the length from 3.1-8.1 feet. The MBP7000 Boom Pole is ideal for Broadcasting, News casting, Film/TV/Video production, School plays/productions, and more field applications.
On Stage MBP7000 Handheld Boom Microphone Pole Features
CALIFORNIA PROPOSITION 65 WARNING
- Standard 5/8 inch-27 threads with solid brass male end and internal 3/8 inch-16 threads
- Ergonomically spaced and comfortable EVA rubber hand grips
- 3 telescopic rods extend the boom length from 3.1-8.1 feet
- Length settings lock/ unlock utilizing a twist-and-lock system
- Ideal for Film/TV/Video production, school plays/productions, and more field applications
WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov.


PULLMAN – As Washington State athletic director Jim Sterk and others search for a new men’s basketball coach in Detroit (see sidebar), the dozen players set to return wait patiently for some idea of their future.
“Everyone wants to be up-to-date as far as what’s happening,” said redshirt freshman Abe Lodwick. “As of right now, I think guys are content to let the administration do their jobs.”
Sterk met with the players earlier this week and assured them he would work quickly to replace Tony Bennett, who announced Monday he was leaving for the University of Virginia.
Sterk assured the players he would listen to their concerns.
“I think it’s important to have opinion be heard and just have everyone be honest about it,” Lodwick said. “We’ve met as a team with (Sterk) and the guys made it clear to him they wanted to have a coach that exemplifies the character that Coach Bennett brought to the team.”
The players elected Lodwick and Nik Koprivica, the only senior on next year’s team, as their liaisons with the athletic department during the search.
“It’s my role to represent the young guys and try to get the best coach we can for our team,” Koprivica said.
“Every guy wants to have input when it comes to (picking) a new coach,” Lodwick said. “But obviously that can’t be possible. So Nik and I will do that for the whole team.”
Lodwick and Koprivica say no one wants to get away from the defensive-first strategy espoused by Bennett, but neither would they be adverse to picking up the offensive tempo.
“I think guys are definitely open to change as far as the way we play,” Lodwick said. “The way we played, obviously, won us games in the past. Defense, half-court offense, that stuff has won us some big games.
“At the same time, there is some really good talent on this team that can excel in different styles.”
Still, as Koprivica said, getting a team consensus on the style played by WSU’s next coach is a near-impossible task.
“You don’t have (the) whole team like one philosophy,” he said. “Somebody plays better run-and-gun and somebody plays better defensively.
“A new coach is going to bring something new to our team and they might help our team play even better. You never know.”
One name that has drawn comment from Sterk is Portland State’s Ken Bone, who guided the Vikings to back-to-back NCAA tournament appearances.
PSU and Washington State held a closed scrimmage prior to this last season, with Portland putting a beating on the Cougars in stretches.
“I like their style, definitely,” Koprivica said. “They went to the NCAA tournament this year which tells me that program is really good. They’ve gone to the tournament two years in a row so that says their coach is really good.
“Their players love him and I think he’s a great coach.”
No matter who is picked, Koprivica said he expects the players to be happy with the choice.
“We would like somebody who’s really experienced and somebody who knows a lot about basketball, who knows about winning,” he said. “But I wouldn’t go into details because I’m going to trust whatever Jim Sterk and President (Elson) Floyd decide because I know they have a lot of experience hiring coaches.
“Whatever they decide is going to be the best thing for our team.”
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View Full Version : [ubuntu] Netgear Dongle
May 10th, 2009, 06:20 PM
I have just installed the latest Ubuntu (v.9.04) on my PC. Our family has a Netgear router, so I was looking at getting a WG111 USB dongle.
Is this supported in v.9 or will it require configuation? I am quite new to Linux, so all comments welcome.
May 10th, 2009, 06:39 PM
I had two WG111 and the best I could do was to get intermittent service. There is a lot on this forum on this subject and you can try to configure this product and after time you may get it to work. I gave up.
The Linksys WUSB54GC works out of the box and I have had good luck with it. However, my computer that uses the usb adaptor is a long way from the router and I get a weak signal. I found a youtube vid on the subject of the Edimax EW-7318USG (Newegg approx $25). This adaptor has an external antenna and is great for weak signals and longer range. It works great.
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text:
SIX Zimbabweans, all suspected armed robbers, yesterday escaped from a South African police custody after their helpers engaged in a shootout with South African police to free them in an incident which occurred outside Louis Trichardt some 120km south of Beitbridge.
The six were identified as Forward Shumba (26), Shingirai Nyandome (32), Brilliant Sibanda (26), Erick Sithole (35), Moses Zambara (32) and Alex Nkomo (35).
They were all prisoners awaiting trial and were being transported by the police when they escaped.
A gang driving a grey Mercedes-Benz vehicle and a white Toyota pick-up truck whisked away the suspects after overpowering police in a shootout that ensued during the escape, the South African Police Services (SAPS) said in a statement.
“The police in Makhado have launched a massive manhunt for a group of heavily armed suspects who attacked and shot at police members transporting six awaiting trial prisoners and managed to free them this morning, Monday 13 (yesterday). The suspects thereafter took the six prisoners and sped off in a grey Mercedes-Benz and a white Toyota bakkie.
“The incident happened along the Vivo road next to the first Schoemansdal turn-off outside Louis Trichardt. The prisoners managed to escape during the attack,” Limpopo province police spokesman Brigadier Motlafela Mojapelo said, adding that all escapees were Zimbabwean nationals.
“The first five were arrested for aggravated robbery, three counts of attempted murder and unlawful possession of firearms and ammunition. The last one was arrested for theft of cables.
“The Provincial Commissioner of Police in Limpopo, Lieutenant General Thembi Hadebe has ordered the mobilisation of the necessary police resources, including the airwing to hunt down the escapees and the suspects.”
- Six Zimbos escape SA police custody after shoot-out
- 6 Zim robbers overpower SA police, escape
- Army officer, Zimbo aided robbers escape
Saps advised the public not to approach the suspects under any circumstances because they are considered dangerous, but should instead call the police.
“Anyone with information may contact Colonel Nyelisani Mabatha +2782 565 8171, the Crime stop number 0860010111 or may send the message via MySAPSApp,” Mojapelo said.

domain: law


By Bennett Oghifo
The fifth edition of the “Business Cars of the Year” awards was held on 24 January 2020 in Paris on the initiative of “Kilomètres Entreprise” magazine. The new SUV PEUGEOT 2008 received first prize in the “SUV Compact Business” category.
The prizes were awarded by a jury of eleven journalists from the editorial staff of “Kilometers Entreprise”. Eight trophies were at stake for a total of 54 vehicles selected from the novelties meeting the criteria of “business” vehicle for professional use in business.
In the “Compact Business SUV” category of 2020, the new PEUGEOT 2008 SUV won the trophy in front of 6 contenders with a total of 42 points, the second receiving 28 points.
“I am very proud to receive this award today, the new PEUGEOT 2008 SUV is starting to market and the first feedback is very encouraging with this vehicle in the B-SUV segment. It attracts by its particularly powerful and athletic style, multi-energies, it offers the freedom of choice for the park managers among three types of motorizations: electric, petrol and Diesel, offering sensations extraordinarily stimulating and efficient. “- Hugues de Laage – Director PSA Corporate Sales France.


text:
The 36 Communes
City of Rivesaltes
Rivesaltes... a name known throughout the world for the quality of its Muscats de Rivesaltes, Rivesaltes and other appellations. Wine growing is not the only agricultural wealth, the quality of its arboriculture and its traditional market gardening far from the forced cultures corresponds well to the current research of natural and true products. The surface area of the commune is 2,876 hectares and its altitude varies between 11 and 70 metres.
The exact date of the foundation of Rivesaltes is not known, but it is thought that the town existed in 729 at the time of the Saracen invasion. Rivesaltes was fortified from 1172. The ensemble probably included seven or eight towers and ditches, which have since been filled in.
Number of inhabitants
The town of Rivesaltes has approximately 8,900 inhabitants. Its inhabitants are called the Rivesaltais.
The Rivesaltes camp memorial
Inaugurated at the end of 2015 and built amidst the remains of the barracks, witnesses to more than 60,000 people, the Rivesaltes Memorial is a place of history and memory, open to the contemporary world.
Its purpose is to restore and transmit the history of this camp, a witness to the conflicts of the 20th century.
It is also a reference point on the issue of forced displacement and its control.

domain: history