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Renewal Bio, an Israeli biotech startup, wants to use the potential of cutting-edge stem cell research to help people live longer and better lives. Through this stem cell technology, the startup is on the mission to create synthetic embryos of people to harvest tissues for medical transplantations.
In this research in stem-cell technology and artificial wombs, the founder of Renewal Bio and biologist at the Weizmann Institute of Science in Rehovot, Jacob Hanna, has started with mouse stem cells where he can form highly realistic-looking mouse embryos and keep them growing in an artificial womb for several days until they develop beating hearts, flowing blood, and cranial folds.
As per Hanna’s research published in the Cell journal, this is the first time an embryo at an advanced stage has been created without sperm, eggs, or even a uterus. Hanna has informed MIT Technology Review that, similar to the rat embryos, he is already working to replicate the technology to experiment with human cells to produce artificial human embryos equivalent to a 40- to 50-day pregnancy in which basic organs and tiny limbs and fingers develop.
The embryo is like a 3D bioprinter that can create natural organs and tissues for Hanna. This endeavor solves the problem of bio-researchers finding it difficult to print complex cell types and organs against simple tissues like cartilage or bone. When a lab-grown embryo can naturally produce organs and tissues, it aids older adults in rebooting the immune system and help older women with age-related infertility by producing youthful eggs.
Omri Amirav-Drory, a partner at NFX that funded the startup with seed capital and the acting CEO, states that he hasn’t seen a technology with so much potential since CRISPR gene-editing technology emerged. Omri stated that the ability to create a synthetic embryo from cells—no egg, no sperm, and no uterus lends to transformative medical technology that can solve concerns related to fertility and longevity.
The whole thing has attracted severe criticism from scientists and regulatory bodies. Stem-cell scientist at the Institute of Molecular Biotechnology in Vienna, Nicolas Rivron, argued that scientists should only create “the minimal embryonic structure necessary” to yield cells of interest.
While there’s criticism, there’s awe-evoking sentiment among other scientists who testify how similar the mouse embryos are to natural embryos. However, techniques for producing synthetic embryos continue to be ineffective. The existing success rate of emulating a mouse embryo is poor, as one in hundreds of attempts would succeed but eventually succumb to defects in heart development due to a lack of sufficient blood supply.
But Hanna’s success with developing mouse embryos at an advanced stage is a breakthrough in stem cell research. He now uses his blood or skin cells (and a few other volunteers) to create synthetic human embryos. He has also assured that these human embryos won’t assume life without a placenta and an umbilical cord connected to a mother.
If need be, Hanna, the scientific founder at Renewal Bio, an Israeli biotech business, said that he will genetically engineer the human embryos to not grow past 40 weeks because the intent is not to develop human clones or to meddle with ethics governing medical sciences, federal agencies, and other regulatory bodies that often decline to fund of synthetic embryo research startups.
Israeli biotech startup Renewal Bio has hired some of Hanna’s students and licensed his technology from the Weizmann Institute. It will begin spending money improving the incubators, developing sensors to track a synthetic embryo as it develops to extend its survival time in the lab. ♦
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domain: medical


This week on Flyover Footy we are joined by Manuel Veth, the regional manager of Transfermarkt USA. Manuel is no stranger to those who follow breaking news in MLS and especially St. Louis. We dig quite deep into the weeds on the pressing style of soccer, Bradley Carnell, standout players, Roberto Firmino rumors, the opening day atmosphere and more.
Flyover Footy has been covering St. Louis soccer since 2015. Subscribe to this feed to hear podcast episodes about Saint Louis FC, St. Louis City SC, college soccer, academy programs, and amateur/semi-pro soccer in the area. This show is broadcast on The Big 550 KTRS on Saturdays at 6pm. Be sure to follow the us on twitter @FlyoverFooty.
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Citing fears about the COVID-19 coronavirus, 72% of U.S. people have radically improved their use of conventional health care solutions, with quite a few delaying in-human being care and embracing digital care, in accordance to a new national study unveiled Thursday by the Alliance of Local community Health and fitness Designs and AMCP, carried out by Leede Analysis.
Amid the respondents, fifty eight% cite their medical professional as the most trustworthy source of data about the virus, but only 31% experience “at ease” traveling to their doctor’s office, main to major adjustments in attitudes and conduct towards typical health care solutions.
What is THE Impression
The adjustments have affected quite a few sides of the health care field. For illustration, 41% of people have delayed health care solutions, whilst 42% say they experience unpleasant heading to a clinic for any sort of medical procedure.
As quite a few as 45%


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Us o Rl90-r V, MTHE CHEVALIER DE MAISON ROUGE. CHAPTER I. THE ENROLLED VOLUNTEERS. IT was on the evening of the 10th of March, 1793 ;ten oclock was striking from Notre Dame, and each stroke sounding, emitted a sad and monotonous vibration. Night had fallen on Paris, not boisterous and stormy, but cold, damp, and foggy. Paris itself at that time was not the Paris of our day ;glittering at night wit...
Paperback: 422 pages
Publisher: Forgotten Books (July 6, 2012)
Product Dimensions: 6 x 1 x 9 inches
Format: PDF Text djvu book
- The Works of Alexandre Dumas, Vol. 11 of 30 (Classic Reprint) epub
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- The Works of Alexandre Dumas, Vol. 11 of 30 (Classic Reprint) text
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Here Take over at midnight the night stalkers pdf link The haunting of hillside school cabin creek mysteries The angerous ol woman Download Howling at the moon book pdf at debtfreemjitsudbas.wordpress.com Here Silent child pdf link Read The apostolic ministry ebook allgeschpunar.wordpress.com Download The pedagogy of confidence inspiring high intellectual performance in urban schools pdf at christiansongshigostam.wordpress.com Janet arnol atterns o ashion
I really enjoyed this story, told as a newspaper article. Recommended....
I read this to my Sunday School. They found the girl's creative use of materials really interesting...

domain: literature


The budget will provide $28.6 million for domestic and international visitor marketing, including for Norther Territory Business Events and Study NT. Marketing efforts will focus on promoting experiences and events in the NT.
The government has allocated $3.8 million to grow capability amongst Aboriginal tourism operators, including upskilling, mentoring and business support.
In addition, $2.1 million will go towards industry development activities, which include continued funding event subvention through the NT Business Events Support Fund.
A further $1 million will go to regional tourism associations in the form of grants for visitor services across the territory.
The government has also indicated the budget will support festival and events.
“Investing in our tourism sector means investing in our future and delivering for Territorians, and will help us deliver a $40 billion economy by 2030,” said the NT’s tourism and hospitality minister Nicole Manison.
“Tourism generates wealth for all Territorians, as visitors spend their money with operators, local retailers and hospitality venues.
“This is good for the economy and good for local jobs, in all regions right across the Territory.”
The NT enjoyed a record visitor spend in 2022 of $3.12 billion. Tourism is responsible for one in every eight jobs in the territory.


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"The Andes Hotel is a hotel and also a popular local restaurant. We ate the restaurant on a bustling Saturday evening. The place was packed, but not..."
"Plattekill is a family style, family owned ski mountain situated in the rural Catskills. We recently spent a brisk early spring day at the mountain skiing and..."
"We planned to have pizza at the Table on Ten in Bloomville New York on a late winter trip to the Catskills. They specialize in pizza on Friday and Saturday..."

domain: travel


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Dhaka: A Bangladeshi court on Wednesday denied bail to 33 opposition leaders, including the BNP`s acting secretary general, charged with masterminding violence during anti-government strike last month that left five people dead.
The leaders of the main opposition Bangladesh Nationalist Party (BNP) and it`s alliance partners were sent to jail after they were denied bail at the heavily guarded court complex at downtown Dhaka.
BNP activists turned violent after the court order when Party`s secretary general Mirza Fakhrul Islam Alamgir and other leaders were whisked to prison vans which took them to high security Dhaka Central jail under heavy security escorts.
Alamgir and the others surrendered before the court in line with a High Court directive after they were charged for masterminding violence particularly for torching a passenger bus in front of prime minister`s office during an opposition enforced nationwide stoppage on April 29.
The other leaders included BNP stalwarts and former ministers Khandaker Mosharraf Hossain, MK Anwar MP, Brigadier General (retd) Hannan Shah, Goyeshwar Chandra Roy and former mayors of Dhaka Sadeque Hossain Khoka and Mirza Abbas.
The court, however, ordered the "division" or privileged status inside the prison for the leaders.
Witnesses said the opposition leaders` counsels including Moudud Ahmed and Barrister Rafiqul Islam Mia told the court that the case against the 18-party leaders was "politically motivated and false" as they sought their bail order.
But, public prosecutor Abu Abdullah vehemently opposed their argument saying their prayer should be rejected as the accused masterminded crimes like arson to destabilise the country in the name of political movement.
The prosecution lawyer said five people lost their lives as the opposition enforced nationwide stoppage and if granted bail they would carryout subversive activities in the name of the anti-government street campaign.

domain: news


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Search results for: Tannenbaum Chana
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This article surveys the literature on learning differences between men and women and discusses the specific implications for the education of Jewish women. It examines learning differences between men and women relating to cognition, personality development and religious identity. The changing role of the modern Orthodox woman and its ramifications for her educational needs are also discussed.
Updated: Sep. 21, 2008

domain: sociology


bed hotel mattress spring-cheap mattress-roll up mattress suppliers bed hotel mattress spring-cheap mattress-roll up mattress suppliers highly promoted by Synwin Global Co.,Ltd has done a great job in managing the trade-off between the practicality and visual appeal. It is noted for its multiple uses and its refined look. Its highly homogeneous surface and fine appearance make it a star design in the whole industry. More importantly, it is its enhanced functionality and ease of use that make it widely accepted.
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All of us understand what it is like to procrastinate. As college students, we put off writing that final paper until the day it is due because we wanted to hang out with our friends. As parents, we’ve watched our children play video games while their list of unfinished chores grows longer. Many of us who work in front of computer screens every day will sometimes delay starting on a new assignment to see what’s happening on a favorite website or trending on Twitter.
Yes, we all know: when there’s real work to be done, we shouldn’t be wasting our time on lesser things. We do it anyway. And so, unfortunately, does Congress.
A prime example is immigration. President Trump and countless Republicans ran on fixing our immigration system. There is no shortage of ideas on how to get the job done: build a wall; build fences; use technology; add manpower; get help from state and local governments; pursue better enforcement policies; shift to more skill-based immigration; strengthen the refugee system, etc. These are the kind of policies that many successful candidates ran on in 2016.
But rather than turn these campaign promises into legislative action, Congress has procrastinated. Talk of border security, enforcement, and legal immigration legislation appears to be fading into the background. Instead of tackling these complex and difficult (yet very important) issues, Congress seems to be preparing to waste its time by first looking to pass an amnesty.
President Trump is wisely winding down the unconstitutional executive amnesty program Deferred Action for Childhood Arrivals (DACA). Yet, instead of taking serious action on the many parts of the immigration system that need fixing, Congress appears hell-bent on ignoring its responsibilities.
And this isn’t the first time. In 1986, Congress passed an amnesty for almost 3 million illegal immigrants. The lawmakers promised that it would be a one-time thing and that they would solve the illegal immigration problem. Instead, they procrastinated. The result: the U.S. now has 11-12 million illegal immigrants — far more than in 1986.
But policymakers and pundits are spinning the same lines they used back then. Amnesty must come first, they say — for the almost 700,000 illegal immigrants in the DACA program, or perhaps even millions of others. The complex issues surrounding immigration? We’ll tackle them later.
Fool us once, shame on you. Fool us twice, shame on us.
Doing the wrong thing only makes things worse. Rewarding illegal immigration with amnesty creates an incentive for even more illegal immigration. It reinforces the notion that, if you come to the U.S. illegally, you will be allowed to stay.
In the case of an amnesty for DACA recipients, the U.S. will be encouraging more individuals to put their children in the hands of smugglers to cross the southern border. Not only is Congress ignoring the issues they promised to tackle, but they are making them worse.
But it doesn’t have to be this way. Congress can quit procrastinating and do the right thing. Let congressional committees work through these complex issues and make improvements to border security, interior enforcement, and the legal immigration system.
Then, after these reforms are in place and proven effective, Congress can turn its attention to figuring out what to do with those still here illegally. That doesn’t mean a mass amnesty is the solution, but merely that the right time for that discussion is after the fundamental issues with our immigration system have been resolved.
Even proponents of amnesty should be able to agree that lawmakers should first fix what’s broken. Rather than amnesty after amnesty, the American people deserve an efficient and well-enforced immigration system.
It’s time for Congress to go to back to work.
This piece originally appeared in The Washington Times


With its fuel-efficient engine lineup,
The Toyota Highlander SUV debuted in 2001. It was one of the first midsize "crossover" SUVs to appear, and Toyota designed it to complement the more rugged and trucklike 4Runner. The Highlander has a carlike unibody design which leads to better handling, less cabin noise, improved crashworthiness and easier entry and exit for passengers.
The Toyota Highlander is meant for people who like the image and versatility of an SUV but prioritize the ride, handling
The four-door Toyota Highlander SUV comes in three trims: base, Sport


In coherent-optical transmission systems continuous or endless polarization matching between the superposed beams must be ensured. We describe a practical closed-loop polarization-control system with two integrated-optical retarders acting as polarization transformers. They have variable retardations and variable eigenmodes, i.e., adjustable birefringence magnitude and orientation as in a Babinet–Soleil compensator. In theory, one retarder would be sufficient. However, a second device is added to correct nonideal behavior of the first. The intensity losses during polarization control are less than 0.15 dB.
© 1988 Optical Society of America
Original Manuscript: November 20, 1987
Manuscript Accepted: March 17, 1988
Published: June 1, 1988
Reinhold Noé, Helmut Heidrich, and Detlef Hoffmann, "Automatic endless polarization control with integrated-optical Ti:LiNbO3 polarization transformers," Opt. Lett. 13, 527-529 (1988)


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1 Postharvest treatment with methyljasmonate affects the antioxidant system and the quality of wine grape during partial dehydration
Margherita Modesti1, Milena Petriccione2, Roberto Forniti1, Luigi Zampella2, Marco Scortichini2, Fabio Mencarelli1*
1Department for Innovations in Biological, Agrifood and Forest Systems, University of Tuscia, Viterbo, Italy
2Consiglio per la Ricerca in Agricoltura e l’Analisi dell’Economia agraria (CREA), Centro di ricerca per Olivicoltura, Frutticoltura e Agrumicoltura, Via Torrino 3, I-81100 Caserta, Italy
*Corresponding Author: firstname.lastname@example.org
BACKGROUND - Postharvest partial dehydration is a widely used technique to produce important wines in Italy, dry and sweet. Accurate management of environmental parameters of dehydration permits to modulate berry metabolism to maintain/improve the enochemical quality of berries. As the water loss induces an oxidative process in berries, we have hypothesized that methyljasmonate (MeJA) and ozone (O3) as postharvest treatment before partial dehydration, could be beneficial for grape berries.
RESULTS Grape bunches were treated with 10 or 100 µM MeJA at 20°C for 12 hours or with ozone gas (20 g h-1 with 6% w w-1 of ozone) at 10°C for 12 hours; control was untreated berries kept at 20°C for 12 hours. Partial dehydration was performed at 10°C, 70% relative himidity (RH) and air flow (1 m s-1) until reaching 30% weight loss
(w.l.). MeJA hastened water loss of grape berries. Total polyphenols and total flavonoids contents were increased by MeJA, immediately, and remained higher than
2 the concentration in control sample but, at the end of partial dehydration, the values were lower than the ozone-treated sample. Superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and guaiacole peroxidase (GPX) increased in partial dehydration, especially in treated samples. Lipooxygenase (LOX) and polyphenoloxidase (PPO) showed lower activity in MeJA samples than in the control.
CONCLUSIONS - MeJA accelerates water loss but, in the same time, activates the antioxidant system. Ozone does not accelerate water loss but activates antioxidant system and increases polyphenol content.
Keyword: Methyl Jasmonate; Ozone; Grapes; Dehydration; Post-harvest.
Postharvest partial dehydration of wine grape is a technique widely used in Italy and in Mediterranean Countries to produce Passito wines, sweet and dry. In the case of dry wine, this wine is considered as reinforced wine because the intensity of weight loss is not so high as for sweet Passito wine. Most of Passito wines in Italy are made from white varieties, e.g. Muscat of Alexandria, Malvasia, Trebbiano, Erbaluce, Picolit and more. Postharvest grape partial dehydration is a complex metabolic system1. Water stress and senescence interact when the environmental parameters of dehydration are well controlled in order to delay water loss, thus slowing down the dehydration process. Practically speaking, the obtained partial dehydration is a real postharvest technology where berry cells are still alive at the end. A cascade of metabolic responses through different and selective modulation of the expression of genes involved in pathways/processes (glycolysis/fermentation, phenylpropanoid, oxidative stress), affects berry composition2. Zenoni et al.1 identified a group of transcripts that were modulated more slowly and gradually during the postharvest period, representing the common features of berries undergoing dehydration and/or commencing senescence. This included genes controlling ethylene and auxin metabolism as well as genes
3 involved in oxidative and osmotic stress, defense responses, anaerobic respiration, and cell wall and carbohydrate metabolism2. The role of oxidative stress, as consequence of water stress during postharvest partial dehydration of grapes, has not been studied but it has been postulated by studying an aerobic fermentation (or anaerobic respiration) taking place during water stress of grapes3, 4, 5 where an increase of oxidized compounds such as acetaldehyde and acetic esters occurred. An up regulation of laccase and peroxidase genes has been observed in the last step of postharvest dehydration1. An increase of antioxidant activity of grapes during off-vine drying has been reported by Moreno et al.6 due to Maillard compound formation. Recently, Panceri et al.7 showed that, antioxidant activity determined by ABTS and DPPH methods, was higher in 40% dehydrated grape than in control or 30% dehydrated samples, and this result was attributed to the increase of polyphenol compounds. The interaction between water stress intensity and rate, seems to play a major role in modulating molecular responses involving not only transcriptional but also post-transcriptional and post-translational regulatory mechanisms2 as well as the environmental parameters such as temperature, relative humidity and air flow8.
Methyl jasmonate is the methylester of jasmonic acid and is synthesized by oxidation, via lipooxygenase, of unsaturated fatty acids of chloroplast membrane9. Jasmonate (JA) and methyl jasmonate (MeJA) are considered phytohormone10. Exogenous application of JAs on different plants under abiotic stresses particularly salinity, drought, and temperature (low/high) conditions, have been effective in improving plant stress tolerance but, also, a variety of JA-induced plant growth events (fertility, sex determination, storage organ formation, reproductive processes, root elongation, fruit ripening and senescence, oxidative defense, and interaction with other hormones) have been reported11. Several studies have shown as the treatment with MeJA increases antioxidant enzyme activity, such as ascorbate peroxidase, superoxide dismutase, glutathione peroxidase, ascorbate dehydroreductase, guaiacol peroxidase, glutathione reductase, and monodehydro ascorbate reductase12, 13, 14, 15. In addition, by
4 considering that this compound is already classified as Generally Recognise As Safe (GRAS) substance by the U.S. Food and Drug Administration16, it may have a potential for enhancing the synthesis of bioactive compounds17. Preharvest and postharvest application of MeJA have produced a greater antioxidant capacity in Chinese red bayberries16, strawberry18 as well as have enhanced anthocyanin synthesis in apples19 or in different berries20, 21, 22, 23, in polyphenols in guava24, 25, and in carotenoids, ascorbic acid and antioxidants in plum26. Treatment with (-) MeJA induced increase in resveratrol, quercetin-glycoside, quercetin-galactoside and quercetin 3-O-rutinoside whereas, a treatment with (+) MeJA provoked an increase in O-glucoside and O-rutinoside, without effects on resveratrol and quercetin-3-O-galactoside content27. Ozone (O3) can be considered a postharvest elicitor because, at right concentration and application time, promote high levels of healthy phytochemicals in fruit and vegetables28, 29, 30, 31 but, generally, as a well-known strong oxidizing agent it is used by the food industry as an antimicrobial agent32. Thus, its positive and negative effects on plant cells depend on the concentration and application time. O3 as strong oxidant decomposes spontaneously producing a mass of reactive oxygen species (ROS)33, 34. If there is an imbalance in a cell compartment between the ROS and the antioxidant defense, oxidative stress and damage will occur35. Some studies have shown an increase in antioxidant enzymes superoxide dismutase, catalase, and ascorbate peroxidase in papaya36 and pear37 to protect fruits from oxidative damage following ozone treatment38.
To our knowledge, the effects of treatment with MeJA on wine grapes subjected to postharvest partial dehydration, have never been investigated. As MeJA has the ability to protect against oxidation and it is known, as explained before, that postharvest water stress induces cell oxidation, our hypothesis has been that a postharvest treatment of wine grape with MeJA before postharvest partial dehydration, could protect or even enhance the quality characteristics of berry for wine production. As comparison we have used ozone postharvest treatment before than postharvest partial dehydration, at
5 a specific concentration we have studied before which resulted nontoxic but beneficial for grape berries; control grapes were untreated before postharvest partial dehydration.
Fruit samples and experimental design
Bunches of white wine grapes cv. Romanesco (about 200 Kg) were harvested in the experimental vineyard of the University located in Montefiascone (VITERBO) in the Lazio region (Italy) (42°29’1.30”N latitude – 12°0’37.37E” longitude). Bunches were hand harvested at about 18 % TSS (total soluble solids) and sorted for color and absence of injuries prior to treatments. Bunches were divided in four lots (about 50 kg each lot). One lot was untreated (control) and bunches were kept at 20 (±1)°C and 70 (± 5) % RH for 12 hours in a cold room. Treatments with MeJA (Sigma–Aldrich. Milano, Italy) were performed at 20 (±1) °C for 12 hours in another temperature controlled room, placing the bunches in small airtight chambers (1 m3) where the appropriated
solution volume of MeJA to reach the desired gas concentration (10 or 100 µM), was deposited on filter paper and the chambers were tightly-sealed. A small fan was placed inside the chamber, to facilitate the MeJA solution evaporation. At the end of treatment, the CO2 and O2 concentrations inside the small chambers were 10% and
12%, respectively. Ozone gas (max 20 g h-1 with 6% w w-1 of ozone) with a flow rate at maximum 150 NL h-1 (NL= normal litre) rate (Ozone generator A series, PC Engineering, Uggiate Trevano, Italy) in a 9 m3 cold room, was used. The treatment was performed at 10 (±1) °C for 12 hours.
After the treatments, all samples were kept in small dehydration tunnels with fans, placed in a cold room at 10 (±1)°C and 70 (± 5) % RH. Air flow of tunnels was fixed at 1 m s-1. Analyses were performed on the first, second and third day after treatments and
then at 10, 20 and 30% w.l.. Physical and Chemical Analyses
Weight loss of 10 bunches each sample was measured by using a technical balance (Adam Equipment Co. Ltd, Milton Keynes, England). Peel color was assessed using a
6 Minolta colorimeter (Minolta C2500. Konica Minolta, Ramsey, NY) to determine chromaticity values L* (Lightness), a* (green to red), and b* (blue to yellow). Initially, 50 berries each treatment were pen-marked and used to perform the colorimetric reading on the same berries for the whole duration of the test. The hue angle (h) was calculated from chromaticity values a* and b* as reported earlier by McGuire39. Peel
resistance was evaluated by using an Instron Universal Testing Machine (model 3343; Instron Inc., Canton, MA) adapted with a needle probe with 1 mm diameter and performed at 1 mm s-1 bar speed. The maximum peel break force (Fsk) was expressed
in N, corresponding to peel resistance to applied break force. For this test, at each sampling time, 60 berries per treatment from different bunches, were analyzed and then used for total soluble solid content using a digital refractometer (ATAGO, Palette PR-32). CO2 production rate was monitored by the gas analyzer HELPY (Marvil
engineering SRL, Bozen, Italy). Bunches were tightly closed for 2 hours into glass jars, adapted with a septum for sampling oxygen and carbon dioxide. One bunch in a glass jar, 3 jars each treatment. CO2 production was expressed as ml kg-1 h-1.
At each sampling time, berries from different bunches (three set of berries) were used for chemical and biochemical analyses. For the following chemical analyses, juices were extracted from the three sets, and used to measure total phenolic content by the Folin–Ciocalteu method40 expressed as mg of gallic acid equivalents (GAE) per 100 g fresh weight (FW) and the total flavonoid content by using the aluminium chloride colorimetric method41, expressed as mg of catechin equivalent (CE) per 100 g fresh weight (FW). For biochemical analyses, berries were immediately immersed in liquid nitrogen and ground to a fine powder in a mortar porcelain.
Extraction and measurement of total soluble protein
Total soluble proteins were extracted after re-suspending frozen fruit tissue powder in extraction buffer (2:5 wv) containing: 100 mM potassium phosphate buffer (pH 7.8), 100 mM sodium EDTA (pH 7), 1.25 mM polyethyleneglycol and 2 mM dithiothreitol. The sample was subsequently homogenized in the mortar with the addition of PVPP to
7 5% and transferred into transferred into a 2-mL Eppendorf tube. The samples were centrifuged at 14000 g for 30 minutes at 4°C. The supernatant was used for measurements of enzyme activities. Protein content was measured by the Bradford assay42 using bovine serum albumin as a standard.
All the enzyme activities were assayed by using the method described by Pasquariello et al.43 with slight modifications.
The reaction medium consisted of 50 mM potassium phosphate buffer (pH 7), 20 mM H2O2 and 100 μL of crude enzyme extract in a final volume of 1.5 mL. The reaction was
started by adding H2O2, and the decrease in absorbance at 240 nm, caused by its
breakdown, was monitored. The specific activity was expressed as nmol H2O2 g-1 fresh weight (FW).
The reaction mixture consisted of 100 mM potassium phosphate buffer (pH 7), 0.25 mM ascorbic acid, 0.70 mM H2O2, 0.66 mM sodium EDTA (pH 7) and 20 μL of crude
enzyme extract in a final volume of 1.5 mL. The reaction was started by adding H2O2,
and the oxidation of ascorbic acid was determined by the decrease at 290 nm. The specific activity was expressed as µmol H2O2 g-1 FW.
The reaction mixture consisted of 50 mM potassium phosphate buffer pH 7.8, 0.1 mM sodium EDTA, 13 mM methionine, 75 μM NBT, 2 μM riboflavin and 100 μL of crude enzyme extract in a total volume of 1.5 mL. The reaction was started by adding riboflavin, and after 15 min of incubation at room temperature under continuous light, the absorbance at 560 nm was measured. One SOD unit was defined as the amount of enzyme that inhibits the rate of NBT reduction by 50% under the above assay conditions. The specific activity was expressed as U g−1 FW.
8 The reaction mixture contained 100 mM potassium phosphate buffer pH 7, 0.20 mM sodium-EDTA pH 7.0, 13,2 mM H2O2, 32 mM guaiacol and 250 μL of crude enzyme
extract in a final volume of 1 mL. Guaiacol peroxidase activity was detected spectrophotometrically by recording the formation of tetraguaiacol and the consequent increase in absorbance at 470 nm. The specific enzyme activity was expressed as as nmol tetraguiacol g-1 FW.
Crude enzyme extract (20 μL) was incubated with a buffered substrate (500 mM catechol in 100 mM sodium phosphate buffer pH 6.4) in a final volume of 1.5 mL and monitored by measuring the increase in absorbance at 398 nm. The specific activity for molar change in catechol was expressed µmol g-1 FW.
The reaction mixture consisted of 100 mM sodium phosphate buffer pH 6, 0.17 mM linoleic acid sodium salt, and 50 μL of crude enzyme extract in a final volume of 1.5 mL. The lipoxygenase activity was detected spectrophotometrically by recording the formation of hydroperoxides and the resulting increase in absorbance at 234 nm. LOX activity was expressed as the specific rate on a fresh weight basis of molar change of hydroperoxides in nmol g-1 FW.
All the data are expressed as the mean ± standard deviation (SD). To determine the difference between uncoated and treated fruit, one-way ANOVA and the least significant difference (LSD) test for mean comparisons were used. Differences at p<0.05 were considered significant and are indicated with different letters. Correlations among the evaluated parameters were analyzed using Pearson’s correlations (p<0.05 and p<0.01). A principal component analysis (PCA), in order to identify the principal components contributing to the majority of the variation within the dataset, was applied to evaluate the effectiveness of different treatments on physical-chemical, nutraceutical
9 and enzymatic traits. All analyses were performed using the SPSS software package, Version 20.0 (SPSS Inc., Chicago, IL, USA).
Partial dehydration process began on 28th of September and, plotting dehydration time versus weight loss (w.l.), two straight lines increased with R2 of 0.9832 and 0.9677, respectively for ozone and control treatment (the former) and for MeJA treatments (the latter) (Figure 1). MeJA-treated grapes showed a more pronounced w.l. rate and the process lasted 27 days while, for the other two treatments, the process was of 50 days. Respiration rate, after 1 day, was significantly higher in MeJA and control samples while ozone showed the lowest value, but this response was to attribute to the different temperature used. Respiratory quotient, after 3 days, was 1.31, 1.08, 1.18, and 1.15, respectively for MeJA 100 µM, MeJA 10 µM, ozone, and control sample (data not shown).
TSS increased during partial dehydration process, without significant difference among the samples, reaching values between 22.3 and 23.4 (Table 1). Peel color values decreased progressively in all samples (Table 2); MeJA samples showed higher values at 10 and 20% w.l. but at 30%, only MeJA 10 µM had a lighter yellow color while the other samples appeared reddish, to indicate an oxidation of peel color. Berry peel resistance increased with the progress of weight loss without significant difference among samples (Table 3).
During the partial dehydration process, a significant increase in polyphenol content was observed (Table 4). Ozone treatment showed the highest values after one day of treatment and at the end of dehydration (30%). MeJA 10 µM induced an increase in polyphenol content on day 1 but, at the end, the concentration was the lowest one. MeJA 100 µM showed an increase in polyphenols on day 2 and, at the end, the value was slightly higher than MeJA 10 µM one. Total flavonoids raised, 1 day after the treatment, in all the treatments but overall in ozone and MeJA 10 µM (Table 5). In
10 control and MeJA 100 µM the value continued to rise until the end of experiment while, in ozone and in MeJA 10 µM, the values declined and rose again at 20% w.l.. At the end, the highest value was for ozone sample.
SOD activity increased after the treatment and during dehydration time. Ozone and MeJA-treated fruits exhibited higher activity than untreated ones (Fig. 2). All treatments stimulated CAT activity, whose values reached a peak in MeJA-treated sample (10 μM), on day 2 after treatment (Fig. 3). APX activity was also induced by treatments especially in the final stages of the partial dehydration process, with higher values in treated samples than in the control. APX activity reached the highest values at 10% w.l. and decreased later on (Fig. 4). During the partial dehydration process, GPX activity increased in all samples and reached higher values in the treated fruits (Fig.5). LOX and PPO activity showed a similar trend in all samples and the activities were higher in control fruit than in treated-samples. LOX activity was constant until the 3rd day after treatment and, then, increased in the partial dehydration process, but no statistical differences were registered among treated samples (Fig. 6). Similar to LOX activity, PPO activity (Fig. 7) increased in all treatments during the partial dehydration process.
The first surprising result has been that MeJA treatments facilitated water loss in grape during the partial dehydration process, reducing the dehydration time (27 days) vs 50 day of ozone-treated and untreated grapes. In another non climacteric fruit such as strawberry, postharvest application of MeJA provoked an induction of ethylene production44 and stimulated ripening45 and, in climacteric fruit, it has been shown the effect of MeJA in hastening ripening26. In contrast, it has been seen MeJA postharvest treatments (1 and 5 M) on eggplant to delay postharvest senescence and consequently to reduce weight loss during 10 days of storage at 20°C12.No specific papers were found on the interaction of MeJA and water loss from plants. So, it is
11 unclear the role of MeJA in weight loss; in our case we can suppose that MeJA has stimulated ethylene production which is known to act on cell wall enzymes of grape berry, favoring the water loss46. In many cases only small amounts of enzyme activity are required to effect significant changes of cell wall polysaccharides during softening of grape berries47 and cell wall enzymes are the very sensitive to ethylene and its activation is one of the first response to small amount of water loss48. Thus, we can speculate that an autocatalytic effect of water loss due to the response in ethylene production as consequence of MeJA treatment and then an induction of cell wall enzymes activity which facilitates the water loss which, as feedback, activates a further cell wall enzyme activity.
This effect of water loss acceleration did not affect ripening parameter as expected as grape is non climacteric fruit; at the end of experiment, TSS in MeJA samples were similar to the ones of the other samples as well the peel resistance and the hue angle.
Total polyphenols and total flavonoids, at the end of experiment, were lower in MeJA-treated berries than in control and ozone sample, the last one showing the highest value. This result of the ozone-treated sample is in agreement with those reported by Artés-Hernandez et al.30 and Carbone and Mencarelli31 who showed that treatments with ozone increase the concentration of phenolic substances on table and wine grapes. As regards methyl jasmonate and polyphenols, an increase of flavonols as a result of treatment with methyl jasmonate on table grapes has been reported57. In our case, we found a significant increase of polyphenols and flavonoids after 3 days from treatment, while, at the end of experiment, the values were lower than control. It is known that, during the postharvest partial dehydration process, the water stress causes an oxidative stress with increase of lipoxygenase and peroxidase4,5. Plant cells have a system of enzymatic and non-enzymatic antioxidants that maintains ROS at the right levels. The enzymes involved in ROS scavenging, such as CAT, APX, GPX and SOD , showed higher activity in the treated samples, overall during partial dehydration,
12 suggesting the antioxidant system is activated in berries as a consequence of water loss. APX activity increase confirms what it has been observed by a proteomic approach in a previous study49 through 2D-DIGE (2-Dimensional Gel Electrophoresis), where an overregulation of this protein during postharvest partial dehydration process of grape (cv. 'Corvina') has been observed. The activity of CAT and APX is directly related to the stress level of samples and, probably, this rise allows for the reduction of H2O2 accumulation in tissue. Previous studies on other fruits such as peach, strawberry and apple have shown an increased activity of these enzymes during postharvest treatment with MeJA12,50,51. In our case, more than the single postharvest MeJA treatment, it is the combination of this treatment and partial dehydration which activates the antioxidant metabolism. The different performance of detoxifying enzymes of hydrogen peroxide during dehydration process, may be due to their different kinetic characteristics. CAT has a high turnover rate and low affinity for H2O2 compared to APX which has a lower Km52,53.This suggests that CAT acts quickly to favour initial cell detoxification, while APX, a very labile enzyme inactivated at high H2O2 concentrations, acts in the advanced stages of partial dehydration process. As regards the stimulatory effect of ozone on antioxidant enzymes SOD, CAT, GPX and APX, the results confirm what observed in papaya36 and in pear37. The effect of ozone is strongly dependent on concentration and time of application; in our case the concentration is low, and the time of application is short, this could have acted as a light oxidizing agent stimulating the activity of these enzymes for the berry cell protection.
In contrast, treatment with MeJA and ozone inhibited the LOX activity, an enzyme which plays an important role in lipid peroxidation process causing damage to cell membrane in plant tissues54,55. LOX has been reported as the first marker of postharvest water stress in Malvasia during postharvest partial dehydration4. In our experiment, already after 1 day, the treated fruits showed a lower level of activity and this difference remained with the progress of dehydration. Our hypothesis is that MeJA might protect cell membrane by inhibiting lipid peroxidation caused by water stress, as
13 demonstrated in other studies56,57. In fact, LOX is responsible for the biosynthesis of JA precursors, consequently MeJA treatment could regulate the synthesis of LOX enzyme. As regards ozone, the decrease of LOX activity is unexpected since ozone is a strong oxidizer but probably, as mentioned above, the low concentration and the short time of application, by activating the antioxidant system and increasing the phenol content, permitted the protection of membrane lipid peroxidation.
Wine grape partial dehydration leads to berry browning58,59 and, in grape berry, the main cause of browning is the PPO activity60. As for LOX, PPO activity increased during partial dehydration and it was always higher in untreated fruits (control). The treatments inhibited PPO activity as reported in other fruits such as pears37 and loquat57.
A MeJA treatment before partial dehydration of wine grape, accelerates weight loss but, in the same time, protects grape from oxidation by activating the antioxidant enzymes, increasing polyphenols and flavonoids until 20% weight loss, and inhibiting LOX and PPO activities.
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Table 1. Total soluble solids measured on the 1st, 2nd, and 3rd day and then at 10, 20, and 30% w.l. during postharvest partial dehydration. Data are the mean of 60 berry readings (± SD). Values with different letters are significantly different (p < 0.05).
21 Table 2. Hue angle measured on the 1st, 2nd, and 3rd day and then at 10, 20, and 30% w.l. during postharvest partial dehydration. Data are the mean of 50 berry readings (± SD). Values with different letters are significantly different (p < 0.05).
Control 18.1±0.6d 18.2±0.4d 18.5±0.3d 20.2±0.3c 20.8±0.3bc 22.3±0.4a Ozone 18.0±0.4d 18.8±0.4d 20.2±0.5c 22.8±0.2a 22.9±0.3a 23.1±0.3a MeJa 10 µM 18.0±0.7d 18.1±0.6d 18.5±0.6d 20.4±0..2c 20.9±0.1bc 23.4±0.4a MeJa 100 µM 18.3±0.3d 19.0±0.6d 19.0±0.2d 20.6±0.4bc 21.6±0.4b 22.4±0.5a
Day 0 Day 1 Day 2 Day 3 10% W. L. 20% W. L. 30% W. L.
Control 92±6a 93±6a 93±5a 52±4d 53±5d 28±3f 26±3f
Ozone 86±4a 89±7a 97±6a 65±5c 57±5cd 28±3f 34±4ef
MeJa 10 µM 88±2ab 85±5ab 86±7ab 97±7a 77±4bc 54±4cd 39±5e
22 Table 3. Peel resistance measured on the 1st, 2nd, and 3rd day and then at 10, 20, and 30% w.l. during postharvest partial dehydration. Data are the mean of 60 berry readings (± SD). Values with different letters are significantly different (p < 0.05).
Table 4. Total polyphenols measured on the 1st, 2nd, and 3rd day and then at 10, 20, and 30% w.l. during postharvest partial dehydration. Data are the mean of 60 berry readings (± SD). Values with different letters are significantly different (p < 0.05).
Polyphenols (mg GAE/100 g FW)
Day 0 Day 1 Day 2 Day 3 10% W. L. 20% W. L. 30% W. L.
Control 130 ±5i 156 ±7h 178 ±6fg 116 ±5l 84 ±4m 189 ±9ef 325 ±11b
Ozone 128 ±7i 306 ±9c 192 ±8e 184 ±8ef 114 ±7l 190 ±4e 377 ±9a
MeJa 10 µM 132 ±5i 225 ±5d 187 ±5ef 167 ±7gh 103 ±5l 218 ±7d 187 ±6ef
MeJa 100 µM 125 ±6i 126 ±5i 216 ±9d 125 ±6i 127 ±6i 191 ±4e 291 ±10c
Peel resistance (N)
Day 0 Day 1 Day 2 Day 3 10% W. L. 20% W. L. 30% W. L.
Control 0.94±0.04cd 0.98±0.06cd 0.98±0.04cd 1.04±0.06ab 1.10±0.04a 1.04±0.03ab 1.04±0.04ab Ozone 0.92±0.02cd 0.92±0.06cd 1.03±0.05ab 0.93±0.02cd 1.06±0.04ab 1.00±0.03bc 1.04±0.03ab MeJa 10 µM 0.94±0.05cd 0.91±0.05d 0.91±0.05d 0.98±0.03cd 1.05±0.02ab 1.05±0.02ab 1.06±0.01ab MeJa 100 µM 0.96±0.04cd 0.98±0.03cd 1.03±0.04ab 0.97±0.02cd 0.98±0.04cd 1.11±0.04a 1.04±0.03ab
23 Table 5. Total flavonoids measured on the 1st, 2nd, and 3rd day and then at 10, 20, and 30% w.l. during postharvest partial dehydration. Data are the mean of 60 berry readings (± SD). Values with different letters are significantly different (p < 0.05).
Flavonoids (mg CE/ 100 g FW)
Day 0 Day 1 Day 2 Day 3 10% W. L. 20% W. L. 30% W. L.
Control 125 ±7o 200 ±11mn 315 ±9l 195 ±7n 325 ±12l 540 ±13f 1367 ±64b
Ozone 123 ±5o 490 ±20g 320 ±13l 416 ±16hi 318 ±10l 754 ±16e 1635 ±33a
MeJa 10 µM 129 ±5o 475 ±10g 223 ±8m 356 ±13i 378 ±9i 910 ±16d 1337 ±27b
Figure 1. Weight loss of grape bunches plotted vs dehydration times. Values are the mean of 3 boxes weights.
Figure 2. SOD activity in grape berries versus mass loss. Values are the mean (± SD) of three enzymatic analyses of three lots of berries from different bunches. Values with different letters are significantly different (p < 0.05).
25 Figure 3. CAT activity in grape berries versus mass loss. Values are the mean (± SD) of three enzymatic analyses of three lots of berries from different bunches. Values with different letters are significantly different (p < 0.05).
Figure 4. APX activity in grape berries versus mass loss. Values are the mean (± SD) of three enzymatic analyses of three lots of berries from different bunches. Values with different letters are significantly different (p < 0.05).
26 Figure 5. GPX activity in grape berries versus mass loss. Values are the mean (± SD) of three enzymatic analyses of three lots of berries from different bunches. Values with different letters are significantly different (p < 0.05).
Figure 6. LOX activity in grape berries versus mass loss. Values are the mean (± SD) of three enzymatic analyses of three lots of berries from different bunches. Values with different letters are significantly different (p < 0.05).
27 Figure 7. PPO activity in grape berries versus mass loss. Values are the mean (± SD) of three enzymatic analyses of three lots of berries from different bunches. Values with different letters are significantly different (p < 0.05).

domain: agronomy


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The Heiress's Homecoming
By Regina Scott
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- Release Date: 2013-03-01
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The Heiress's Homecoming Details
Terms of the Will
To keep her cherished childhood home, Samantha Everard must marry by her twenty-fifth birthday. Yet she refuses to marry on a whim, not even to save her fortune. When she returns to Dallsten Manor to say goodbye, the last person she expects to see is her handsome, disapproving neighbor William Wentworth, Earl of Kendrick.
Will is certain the scandalous Everard family is nothing but trouble. He shouldn't care about Samantha's predicament, but her feistiness and kindheartedness intrigue himas does her refusal to wed. He wants to help, especially when he perceives the threat that surrounds her. Soon his greatest wish is to persuade Samantha that her true home is with him.
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Citing an unnamed source, Dow Jones said yesterday that PAI Partners is in discussion with investment banks about a possible sale of the French dairy giant.
PAI, which also owns stakes in dairy ingredients supplier Chr. Hansen and snack firm United Biscuits, bought a 50 per cent holding in Yoplait back in June 2002 from the French dairy cooperative Sodiaal. The co-op retained the remaining share in the business.
At the time, PAI said it intended to build the strength and global presence of Yoplait, investing in product innovation and branding.
In partnership with Sodiaal, PAI succeeded in growing the global turnover of the business from about €2bn in 2002 to over €3.5bn last year. Yoplait is now the second largest brand of fresh dairy products in France and commands a considerable presence in overseas markets. It has a presence in almost 50 countries and North America accounts for almost 50 per cent of its gross turnover.
The Financial Times said Yoplait is likely to attract interest from foreign dairy rivals like Nestle, General Mills, and Arla Foods and could be auctioned off for as much as €1.2bn.
Yoplait is not the only business PAI could be putting up for sale. According to press reports, United Biscuits, which is owned jointly by PAI and fellow private equity group Blackstone, is also being put up for sale.
In its report, the Financial Times said owners of food assets consider that now is a good time to sell because food is seen as a safe bet in uncertain economic times.

domain: finance


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Before 2015, Gucci was experiencing difficulties as the brand struggled to shed the image of being a brand for older people. Things turned around when business executive, Marco Bizzarri was instated as the president and CEO of Gucci, and the brand was able to grow rapidly, targeting a much younger demographic.
During an interview with the CEO, Marco mentioned EXO's Kai, who has been selected as Gucci's global ambassador for three consecutive years. In celebration of the 100-year-anniversary of the brand, Gucci even launched a special collaborative collection with Kai.
Marco Bizzarri stated, "The relationship between Kai is special. That is because Gucci is a special brand and Kai is a special individual. Kai is also a person who passionately reads. Kai's style is very unique and modern. We can also feel the absolute freedom through his dance, and that is aligned with the values of Gucci."
As soon as Marco Bizzarri was instated as the CEO, he recruited Italian fashion designer, Alessandro Michele, as Gucci's creative director. Ever since being appointed as the creative director, Alessandro Michele was able to produce classic yet hip designs to revive Gucci's image.
Even the creator director praised Kai saying, "Kai's facial expression is so dynamic that stirs up one's imagination and has the power to bring out various reactions from the public."
Korean netizens couldn't help but agree with the praise that the executives of the luxurious brand had given the K-pop idol member. Many netizens also praised the oneiric vibe that Kai exudes in various pictorials that he had been featured on.
Netizens commented, "I can understand why the CEO of Gucci is so fond of Kai," "I'm really proud to be a Korean because Kai is cherished by the executives of such a large brand," "It's amazing that Kai is the global ambassador for Gucci," "I'm so proud that a Korean is the face of such a luxurious brand," and "I love how Kai's face is all over Gucci pictorials and advertisements."

domain: artistic


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Across the street from the is a colorful shop that sells more than 15 flavors of one of the world’s top-selling ice creams.
It's been a popular stop for local families for the past 40 years, summer, winter, spring and fall.
“Whether it’s snowing or raining, they don’t care,” owner Joseph Rollakanty said.
“In the evenings, families like to come,” Rollakanty said. "They have children? They got the promotion? They want to treat somebody? We’re a happy go-to place.”
Located at 3004 Hamilton St., is consistent and affordable. Rollakanty, who has owned the Hyattsville store since 2007, says he’s noticed a trend—generations of the same families stopping by.
“A lot of [people] will come and say, ‘Oh, when I was small my family used to bring me here,'” he said. “They come here because of quality and flavor. They don’t care about the prices. Before they come in, they come mentally prepared.”
Prices aren’t an issue at this Baskin-Robbins, however. Rollakanty claims to have some of the cheapest menu items in the Washington Metropolitan area.
“You see my kid’s scoop? It’s $1.99,” he said, pointing to the menu on the wall. A kid’s scoop of ice cream costs $2.69 in Takoma Park and $2.19 in Southeast Washington, D.C. “They say, ‘You’re crazy, you won’t make money.’ I just want to make Baskin-Robbins affordable to as many people as possible.”
Most of Rollakanty’s employees are students at local high schools and colleges who are new to the work force. He sees hiring young people as a good investment in the community and for the students’ futures.
“I not only give them jobs but also teach them how to build character," he said. "I make sure they have a good foundation. They learn how to talk to people."


A bill is being discussed in a Kansas Senate committee Tuesday that would give fire investigators law enforcement powers in the state.
This means, if an investigator encounters a crime in progress, they would have the legal right to pull-over, detain, and even arrest someone. The Kansas Fire Marshal, Doug Jorgensen, made clear during his testimony in favor of the bill that investigators would not be conducting traffic stops.
– PUB DATE: 3/11/2020 12:00:00 AM – SOURCE: KSNT-TV NBC 27 Topeka
Source: SEAFC National Fire News


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- About us
- Degree studies
- Exchange studies
- Art Research & Innovation
- VAA galleries
Artistic initiative of solidarity with Ukraine by Valentyn Odnoviun
Over 35 thousand euros were raised for organisations supporting Ukraine.
Professor led creative workshops to students from Faculties of Vilnius and Telšiai.
The creative workshop was initiated and led by dr. Marius Daraškevičius.
Interested in spending time at Nida Art Colony and getting insights on its operations?
Exhibition celebrating the symbolic 30th anniversary of the VAA Department of Fashion Design is open until 1st June at Panemunė Castle, Vytėnų str. 53, Pilis I, Jurbarkas district.
The workshop was organised in cooperation with the Vilnius Faculty Department of Graphic Art.
Students were led by assoc. prof. Saulius Jusionis
The creative workshop was organized and led by prof. Žilvinas Lilas alongside Ignas Pavliukevičius and Egis Bachur.
An exhibition is open at the library of VAA, Maironio str. 6, Vilnius.
Lecturers of Department of Graphic Design participated with delegates from United Kingdom, Spain, Iceland and the Netherlands.
Workshop was organized and led by assoc. prof. Saulius Jusionis alongside prof. Alvaro Rojas and prof. Fredy Massad.


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After the leftover turkey has been put away and the last of the relatives has been shipped off, there is a good chance you will be ready to step out on the town. Why not relive your recent holiday memories and have a laugh at the same time? The Inwood Theatre, 5458 West Lovers Lane at Inwood, is showing the Thanksgiving neo-classic Home For the Holidays
at midnight on Friday and Saturday. Holly Hunter, Anne Bancroft and Robert Downey Jr. star in this 1995 comedy, directed by Jodie Foster, about a uniquely dysfunctional family. Hunter plays Claudia, who is divorced and 40 and has just lost her job when she arrives at the chaotic home of her parents for Thanksgiving. Between her uptight, angry sister, her trouble-making gay brother and her overbearing mother, the holiday becomes one disaster after another. The humor plays out well, and it is sure to make you feel better about your own family. Call 214-764-9106 or visit landmarktheatres.com
for more information.
Sat., Nov. 26, midnight; Sun., Nov. 27, midnight, 2011

domain: entertainment


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Chances are very few people have heard of St Pierre and Miquelon (StP&M).
They are two small islands off Southern Newfoundland near the Grand Banks and their main claim to fame is that they are part of France. Indeed, they even send a Senator and Deputy to the National Assembly in Paris!
SP&M was the subject of a brief flurry of attention in the early days of World War Two. Following the fall of France, the islands’ Governor declared for Vichy, the collaborationist regime.
De Gaulle’s Free French based in London, desperate to assert themselves but fairly weak (militarily) at that stage, decided to seize the islands.
Being sited as they were so far from the main action of the war at that time it was a safe bet! However, the US government took exception and for a brief time there was a stand-off between Washington, London and De Gaulle. Eventually matters resolved themselves and StP&M relapsed into obscurity and is still part of France.
The French go to great lengths to retain and maintain their scattered pieces around the world. StP&M, like Reunion Island many thousands of miles from France in the Indian Ocean, can be reached by direct flight from France courtesy of large international airports built at some expense (although in the case of StP&M flights still route via Canada).
The French therefore make no pretence of hanging on to their colonies – it’s a case of ‘they’re ours and we’re keeping them’!
The British on the other hand are more subtle. Their overseas territories and dependencies are ‘not really part of the UK, they have their own distinct identities’ and have ‘so called democratic governments’. (Pause for chuckle).
I was minded to think about this some weeks ago when Speaker Steve Rodan and some colleagues from the Commonwealth Parliamentary Association (another absurd organisation) packed their buckets and spades and headed off to the British Virgin Islands.
However, they were not going for the sun, sand and the ‘exotic’ scenery – this was ‘serious stuff’. Their role (I’m not making this up) was to be part of an ‘Election Observation Mission’.
So, just so you get this right, ‘politicians’ from the CPA including two from ‘pretend parliaments’ (namely Isle of Man and Turks and Caicos) were having a Caribbean jaunt to ensure that ‘democratic parliamentary norms’ were maintained in another ‘pretend parliament’, the BVI.
The democratic credentials of this jaunt were exposed by the fact that they had ‘been invited by the BVI ‘Governor’ – democracy in action!
It is long past the time when people grew up and realised that all this ‘bull’ about the Isle of Man being one of the world’s oldest parliamentary democracy is just nonsense.
The Isle of Man no more controls its own destiny than StP&M, the only difference being that, unlike the residents of those overseas French outcrops, we get to pay the tab for building and running our own airport and generally the people in the ‘National Assembly’ in London are more interested in running us down than defending our interests.
The Manx government could, if it wanted, stop being a ‘pretend parliament’ and become the real thing. But a lot of the current crowd in Tynwald don’t want that.
Vociferous among those is our island-hopping Speaker Steve ‘Mc’ Rodan. Indeed, in 2011 Rodan stressed the value of our links with the UK when the UK were keen on ending one of those links: the Reciprocal Health Agreement.
Unlike those French islands I mentioned earlier, the UK government do not like funding anything in the Isle of Man. The Reciprocal Health Agreement was maintained but eventually it will be severed. The VAT agreement lurches from crisis to crisis.
The UK screw every penny they can out of us, be it for defence (?), common services, higher education (that’s overseas rates, so bring the Manx government cheque book) and specialist health care (again, keep that cheque book handy).
Why don’t the Manx government shake off their dependency culture and stop living in a pretend world with a ‘pretend parliament’?
Have the real thing and tell the United Kingdom to take its governor and all the rest of its colonial baggage and ‘get the boat’.
Either that or settle for the StP&M option.
At least that’s more honest!

domain: geography


Brendan Keenan: Once again, Ireland finds itself a mere pawn in a much bigger game
William or James; Wellington or Napoleon; King or Kaiser? The story of Ireland often seems to be that of a long-suffering pawn caught in the struggles of great powers. It feels a bit like that again.
It is not a comfortable feeling. Reading Irish history is often uncomfortable for a modern Irish citizen. No democrat could be on the side of the Stuart autocrats or the Prussian militarists but they were our enemy's enemy, and therefore our friend.
By that measure, if the EU Commission's competition arm is the enemy of Apple, does that make Apple our friend? Ever since the ruling was published, many Irish people have been writhing in discomfort at that thought.


Date(s) - Feb 26, 2023 - Feb 28, 2023
9840 International Dr.
32819 United States
2023 SERC | Southeast Recycling Conference
Stop by and meet our industry experts and learn about GK’s innovative equipment solutions for the recycling industry.
Click here to learn more about the show We are located at Booth #26
Make sure you add us to your show planner!


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Watch the four 30sec ads that Goldman Sachs is rolling out for their new consumer lending offering, Marcus.
My favorite is the Interest Rates Are Not Created Equal!
The ad agency that produced these is the same one used by Apple.
Which is your favorite?
What do you think of the messaging?

domain: economics


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Wherever you go, whatever you do, I will be right here waiting for you.
This is inevitable.This seems to answer my doubts.Love is like the moon, when it does not increase, it decreases.
For me, Xiao Xiong is not only a major event, but also may change my life.This makes us think deeply.Someone said a famous quote if you find a job that you enjoy, then you won't have to work another day.
In this inevitable conflict, we must solve this problem.This inspired me how to develop next.With him, other people in the world can be ignored.
The occurrence of Xiao Xiong, in the end, how to do, not the occurrence of Xiao Xiong, how to produce.With these words, we have to examine this issue more carefully!Hope this second is our world, reincarnation in your side.
We have to face a very embarrassing fact, that is,Although this sentence is very short, it makes me think about it.i'll love you as long as i live.
Let's think about it this way,I hope everybody can also understand this sentence well.I love you not because of who you are, but because of who I am when I am with you.
Then, the next problem is easy to solve.With these words, we have to examine this issue more carefully!if the sun were to rise in the west, i'd never change my mind to love you forever.
From this point of view,This seems to answer my doubts.Don't forget the things you once you owned. Treasure the things you can't get. Don't give up the things that belong to you and keep those lost things in memory.
In that case,This sentence brings us to a new dimension to think about this problem.Never stop smiling, not even when you're sad, some man fall in love with your smile.
We should be clear about the existence of Xiao Xiong.This sentence brings us to a new dimension to think about this problem.Bernara Shaw Once said, The people who get on in this world are the people who get up and look for circumstances they want, and if they cannot find them, make them.
Xiao Xiong, what will happen, what will not happen.This sentence brings us to a new dimension to think about this problem.

domain: psychology


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Connie Maxwell President to Speak at Fall Graduation
On Thursday, Dec. 10, North Greenville University will hold its December graduation ceremony at Turner Chapel, featuring guest speaker Rev. Randy Harling.
Harling currently serves as president of Connie Maxwell Children's Home in Greenwood, S.C. Prior to his tenure at Connie Maxwell, Harling pastored at First Baptist Church Simpsonville for more than 15 years.
Under Harling's leadership, First Baptist Church Simpsonville began a ministry called A Home for Me, which helped to connect churches and the South Carolina Department of Social Services in operating foster homes.
Harling has also worked with other churches in establishing their foster and adoption ministries and with the Columbia-based nonprofit Children Come First. He and his wife Sherri have personally fostered four boys, in addition to raising two sons of their own.
Harling will present the main message at the graduation ceremony, as well as receive an honorary doctorate from the university.
NGU's fall 2015 graduation ceremony is scheduled for Thursday, Dec. 10, at 2 p.m. Tickets are not required. Doors open at 1 p.m.
Connie Maxwell Children's Home former president Dr. Ben Davis, left, congratulates Randy Harling, who succeeded Davis as president of the South Carolina Baptist Convention-affiliated ministry in September. Image Credit: Connie Maxwell Children's Home.
NGU Service Awards 2015
Office of Enrollment Services honors "You Make a Difference Award" winners
NGU Music Ed Alum Sells All to Teach, Serve Overseas
Ashley O’Shields (’04) boarded a one-way flight to Cambodia in July 2016.
NGU wants your Feedback on Our University Magazine
Give us your feedback on the NGU Magazine.
Fall 2016 Dean's List
Congratulations to these students who have been named to the Fall 2016 Dean's List.
500th Anniversary of the Protestant Reformation Sparks First Lectureship
Inaugural lectureship begins with Martin Luther overview
2017 Summer Camp Information
Women's soccer, women's volleyball, and football are offering several camp opportunities this summer for children of all ages.
NGU Orchestra to Present Concert
Program to include NGU ROTC Cadets
Biological Honor Society installed at NGU
Tau Delta Alpha chapter was inducted as well as 25 new members


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The California Air Resources Board’s (CARB) regulation to eliminate internal combustion engine (ICE) forklifts will cost California forklift owners and operators up to $27 billion, according to a new economic impact report released by the Western Propane Gas Association (WPGA).
“Throughout the rulemaking process, CARB did not adequately account for the overwhelming costs and impacts associated with its zero-emission forklift proposal,” says Colin Sueyres, president and CEO of WPGA. “It’s critical that CARB take a hard look at the real-world impact of its proposal and work with small businesses throughout the state who have proposed a cheaper, more feasible, and more effective way to meet the state’s air quality goals. Additionally, local communities and others can’t afford to be hit with unintended consequences not accounted for.”
- The regulations bring on increased costs that directly impact local communities, small businesses, food banks, non-profits, state agencies, and local governments. While some costs and impacts can be anticipated with the passage of this rule, there are unintended consequences that have not been accounted for that would have a rippling economic effect across the state.
- CARB vastly underestimated the number of forklifts that will impacted by the rule. CARB estimates 95,000 forklifts will be affected when in reality 220,000 (more than half of all forklifts in the state) will be impacted.
- $10 billion will be spent on ICE forklift replacements. Forklift owners will be required to replace ICE forklifts even if they are fully functional. Utilization loss caused by the proposed regulation will reach approximately $4.6 billion by 2038.
- By 2038, forklift battery costs will exceed $2.8 billion. Charging stations will costs will exceed $6.3 billion to implement. This cost does not factor in the cost of building power supply upgrades, or infrastructure upgrades for the generation, transmission and delivery of electricity.
- Costs to the state government amount to $33 million by 2038. The State of California, not including the UC and CSU systems, currently owns and operates 581 affected ICE forklifts.
- The total number of battery electric forklifts sold across North America (including the United States, Canada, Mexico) in 2022 was approximately 225,000. If CARB’s rule goes into effect, the majority of battery electric forklifts being sold in North America would – out of necessity – need to be sold within California just to keep pace with the implementation phase-in of the rule. This also does not address manufacturers' ability to scale battery electric production in a global marketplace where battery minerals become more coveted, expensive, and scarce.


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Representing JesusIn what practical ways can you effectively represent Christ?
Top Music Videos
Chris Tomlin Your Heart (DAVID) (Slideshow With Lyrics)
Official Slideshow with Lyrics for Your Heart by Chris Tomlin. The song is performed from the perspective of David. From the album Music Inspired by The Story. Buy here: http://www.thestorycd.com


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In continuation of efforts to check sharp practices in the downstream sector of the oil & gas industry, the Department of Petroleum Resources (DPR) during a routine surveillance drive carried out under the Eket Field office, discovered that a depot operated by Hudson Petroleum Limited in Calabar, Cross River State was hoarding PMS.
DPR investigations revealed that despite receiving 13,606,064 litres of the product in the depot late in December 2015, the operator still contrived to load out very sparingly and was in addition selling at N95.00 per litre, thereby violating Government’s stipulated ex-depot price of N76.50k.
Being mindful of the need to ensure availability of products, especially PMS to the public at all times in order to ease attendant hardship, DPR made several attempts to compel the depot to increase the volume and frequency of truck-out and also to revert to the official ex-depot price, but the company persisted in this unwholesome practice.
Consequently, the depot has been placed under seal specifically for selling above the regulated depot price and for hoarding of PMS. It has been mandated to pay the punitive fine of ten million naira (N10,000,000.00) only, and in addition, the company has been suspended from operations for 3 months in accordance with the new sanctions regime recently issued by DPR.

domain: law


Friday, January 24 Estonian Music Awards gala took place where awards were given in 18 categories. Anna Kaneelina was nominated in four categories and received all the four awards. Very proud!
- Album of the year
- Female artist of the year
- Alternative/indie album of the year
- Debut album of the year


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Label: Delmore Recordings - SG-10 • Format: 2x, CD Album, Remastered Papersleeve • Country: Japan • Genre: Blues, Folk, World, & Country • Style: Folk
Mark Twain (2) - Die Abenteuer Des Huckleberry Finn (1) (Vinyl, LP), Gdyby Nie Niemce - Smagalaz - Czillen Am Grillen (CD, Album), Dr Who - Various - Ultimate TV Themes (CD), Blue Nile, Chase The Sun - You Gotta Go (File, MP3), Fear No Evil (Instr.) - Automatic (18) - Fear No Evil (Vinyl), Zé Ketti* - Opinião...E Outros Sucessos (Vinyl, LP), Mississippi Sawyer - L.A. Suess - Memories (Cassette, Album), 9 AM (Disco Trash Music Remix) - Play Paul - 9 AM (Vinyl), Tribute In Prayer - Rev. Oris Mays - Tribute In Prayer (From A Soldier In Viet Nam) (Vinyl, LP, Albu, Distributor Logo #2 - OH/EX/OH* - The House In The Woods (CDr), Untitled - Scott A. Cooke - LF-Oids Volume 1 Bassline Loops (CD), Hummer - Smashing Pumpkins* - Whatever (CD), Eurofestival 69 Pt.2
Discogs で Karen Dalton - Cotton Eyed Joe に関するリリース、レビュー、クレジット、楽曲などを発見し、Karen Dalton のコレクションを完成させましょう。.
Cotton Eyed Joe, Vol. 1, an album by Karen Dalton on Spotify. Red Are the Flowers - Live. 7. Blues on the Ceiling - Live. Joe (Live in Boulder ) In My Own Time. It's So Hard To Tell Who's Going To Love You The Best. More Karen Dalton. Listen to Cotton Eyed Joe, Vol. 1 now. Listen to Cotton Eyed Joe, Vol. 1 in full in the.
Discover releases, reviews, credits, songs, and more about Karen Dalton - Cotton Eyed Joe at Discogs. Complete your Karen Dalton collection/5(15).
Including for the first-time ever on Vinyl - 3 LP: the double live album Cotton Eyed Joe remastered. + the home recordings album Green Rocky Road remastered - 4 CD: same albums + 1CD of unreleased home recordings including a mesmerizing take on "God Bless The Child" - 52 PAGE BOOK with scans of KAREN DALTON PERSONAL ARCHIVES (writings, photographs, memos) .
Aug 24, · 50+ videos Play all Mix - Rednex - Cotton Eye Joe (Official Music Video) [HD] - RednexMusic com YouTube 12 Year Old Boy Humiliates Simon Cowell - Duration: LosGranosTV Recommended for you.
Cotton Eyed Joe is a live album by Karen Dalton recorded in October , but not released until , in 2×CD + DVD format. At the time Dalton, her husband, and daughter lived in a shack in the Colorado mountains, without electricity or running water, and she would occasionally play at the Attic, a folk club in Boulder, hedringraloguduvemanepercuacheek.coinfo: Folk blues.


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Farms and Agricultural Businesses Now Eligible for Economic Injury Disaster Loans
The Small Business Administration (SBA) announced on Monday, May 4th that farms and agricultural businesses would now be eligible to apply for and receive Economic Injury Disaster Loans (EIDL). The EIDL program is designed to assist businesses affected by natural and economic disasters. Often used for events like hurricanes and earthquakes, the SBA has declared all 50 states, Washington, DC, and several territories are eligible to receive EIDL loans for businesses affected by the coronavirus pandemic.
The SBA had previously been prohibited from awarding loans to agricultural businesses, but the Paycheck Protection Program and Healthcare Enhancement Act, passed by Congress two weeks ago, authorized the use of SBA funds for these businesses. The EIDL awards loans of up to $150,000 to cover debt, payroll, and other expenses. The program recently awarded up to $2 million but reduced the maximum amount under budgetary pressure and a glut of applications. The loan can be repaid over as much as 30 years at a 3.75% interest rate (or 2.75% for not-for-profits). EIDL awardees are also eligible for a $10,000 advance to cover immediate expenses. The $10,000 advance is a grant and does not need to be repaid.
Agricultural businesses with 500 or fewer employees will be eligible to apply. The SBA stopped accepting EIDL applications on April 15, and it will now process those applications on a first-come, first-served basis. Non-agricultural businesses are no longer eligible for EIDL loans, although they are still eligible for other SBA loan programs, including the Paycheck Protection Program.
Agricultural businesses that have already applied for an EIDL loan do not need to reapply now. Those businesses can begin their loan applications here. For more information on SBA loan programs, see this previous O’Neill and Associates blog post.


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Without wasting time, Mavin Records uber-talented singer, Korede Bello premieres the music video to his latest titled “Mr Vendor”.
The new song is a follow-up to his recent record ‘Joko’, featuring Miya B and Fresh Prince.
Video shot and directed by Kaz Ove.
Watch and share your thoughts below.
Call +2348178250300 or Chat with us on Whatsapp For Music Promo Only

domain: celebrity


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LUPO POLO Stretch Piqué Dyed - Slim Fit
View Size Guide
Inspired by a vintage polo from the 1950s found in the DENHAM Garment Library, the LUPO Polo is made from dyed jersey. The ribbed, rigid collar enhances the authentic look of the polo, which has been dyed in our seasonal spring/summer 2020 colours. The overall clean-cut look of this short-sleeved polo is finished off with DENHAM’s signature scissor embroidery on the chest and logo in the lefthand corner.
LUPO POLO SPQD

domain: design


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Must. See. Show. Don’t miss out. More at remindmetomorrow.com.
After Sleigh Bells wrap up their extensive Florida tour with Diplo and Brooklyn’s Liturgy, the noise-pop duo will be playing select North American shows around the release of Reign of Terror.
02/16 – Washington, DC @ 9:30 Club
02/17 – New York, NY @ Terminal 5
02/18 – Toronto, ON @ Phoenix
02/21 – Los Angeles, CA @ Music Box
02/23 – San Francisco, CA @ Regency Ballroom
02/24 – Seattle, WA @ Showbox at Market
[mtv width=512 height=319]726483[/mtv]
Sleigh Bells//Comeback Kid (from Reign of Terror)
Over the weekend, Sleigh Bells dropped the first official single from their soon-to-be-released LP, Reign of Terror. Though I still enjoy “Born to Lose” a bit more, the new single, “Comeback Kid”, is yet another impressive track and keeps me hopeful that Sleigh Bells sophomore outing will live up to my high expectations.
Reign of Terror is out Feb. 21st and you can catch Sleigh Bells on tour, starting Feb. 3rd.


The energy transformation ("Tier III") provisions of Act 56, passed by the Vermont Legislature in 2015, set targets for reducing consumption of fossil fuels and production of greenhouse gases. Electric utilities have been tasked with implementing programs to help their customers transition from fossil fuels to electric or other renewable sources. The requirement starts in 2017 at 2 percent of electricity sales (BTU equivalency) and rises each year until it reaches 12 percent in 2032. To be eligible, an energy transformation project must result in a net reduction in fossil fuels.
The programs that Co-op is offering through the Energy Transformation Program in 2017 are outlined below. Click here to read the full version of VEC's 2017 Plan.
Cold-Climate Heat Pumps
Heating contributes about a third of Vermont’s carbon emissions, making it an important target for carbon reductions. Cold-climate heat pumps provide an opportunity for Vermonters to reduce the amount of oil or propane they use to heat their homes. VEC is partnering with Efficiency Vermont to offer VEC members an extra incentive to install cold-climate heat pumps in their homes and businesses. In 2017 all VEC members who install a cold-climate heat pump will be eligible to obtain a one-time $150 bill credit in addition to the Efficiency Vermont's incentive, which reduces the cost of installation.
- Click here to learn more about heat pumps from Efficiency Vermont.
- Is a cold-climate heat pump right for you? Click here to learn more from Efficiency Vermont.
- To obtain a $150 VEC bill credit, complete and submit this form.
Electric and Plug-in Hybrid Electric Vehicles
The transportation sector contributes nearly half of Vermont’s greenhouse gas emissions. Electric vehicles (EVs) and plug-in hybrid electric vehicles (PHEVs) offer opportunities to reduce carbon emissiones, and the technology has continued to improve with better range and cold weather performance. As a means of promoting EVs and PHEVs, VEC will offer a $250 bill credit for the purchase of new or used EVs and PHEVs purchased in 2017. Members who lease an EV or PHEV in 2017 will be eligible for an upfront bill credit of $50 for each year of the lease.
- Is an Electric Vehicle or Plug-in Hybrid Electric Vehicle right for you? Click here to learn more from Drive Electric Vermont.
- To obtain a bill credit, complete and submit this form.
Clean Air Program


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I'm seeking a published writer or writing instructor to moderate and participate in a new Morningside Writers Sci-Fi/Spec Fiction Workshop.
There are currently three female writers in the Commercial/Literary Fiction Workshop that would migrate into the new workshop. Ideally looking for three male writers (including moderator), but flexible as long as there is a gender balance.
Each workshop has a maximum of six writers who are workshopped twelve times a year, in addition to writing exercises and career guidance lectures.
If interested, send a cover letter, resume, and writing samples or links to writing samples: firstname.lastname@example.org