The 9 Best Chillow Cooling Pillow
Nowadays, there are so many products of chillow cooling pillow in the market and you are wondering to choose a best one. You have searched for chillow cooling pillow in many merchants, compared about products prices & reviews before deciding to buy them.
You are in RIGHT PLACE.
Here are some of best sellings chillow cooling pillow which we would like to recommend with high customer review ratings to guide you on quality & popularity of each items.
Classic Brands Reversible Cool Gel and Memory Foam Double-Sided Pillow, Soft and Comfortable Orthopedic Support, Standard
- A must have reversible cool gel and memory foam pillow
- cool gel layer on one side for warm nights and memory foam on the other side for cooler nights
- Product dimensions 16 L x 24 W x 5 inches H
- Soft memory foam cradles your head and holds your neck and shoulders in an optimal sleeping position and retains shape night after night
- Cool gel memory foam regulates temperature, disperses heat and promotes airflow keeping you pleasantly comfortable all night
- 5 inch loft is ideal for side, back and stomach sleepers.Naturally antimicrobial
- Medium plush feel
- Hypoallergenic performance mesh knit cover is removable and machine washable
- 5 Star Customer Service
- Available 7 days a week
A must have reversible cool gel and memory foam pillow, the perfect combination of soft conforming memory foam and cool gel layer. The Classic Brands Cool Reversible Gel and Memory Foam Pillow responds to temperature and pressure and molds to your shape by creating the perfect comfort for your head and proper alignment for your neck, shoulders, and back. No need to flip the pillow to find a cool side while you sleep. The silky performance knit cover is breathable and allows air flow through, wicking away moisture so that you can rest easy and comfortably while you sleep.
The perfect combination of conforming memory foam and cooling gel! Memory foam surrounds a pad of Cool Gel material giving you a cooling sensation while you sleep, eliminating the need to flip the pillow to find a cool side. The Cool Gel Memory Foam Pillow responds to temperature and pressure to mold to your shape by creating the perfect support for your head, neck and shoulders. Allergy sufferers love our memory foam pillows because they are naturally hypoallergenic.
The Latest Open-Cell Memory Foam Technology
Open Cell Memory Foam promotes airflow through the pillow – this next generation of memory foam is more porous or open than conventional foam, meaning the cells are interconnected unlike closed cell foam; thereby it is more breathable and comfortable than higher density visco-elastic foam. The open-celled physical structure of our foams is self-ventilating. Air is able to travel throughout the material and actually dissipate body heat regulating your body temperature. Memory foam is naturally hypoallergenic and resistant to allergens, mold, bacteria and dust mites.
Benefits of Cool Gel
Cool Gel keeps you cool while you sleep – the Cool Gel side disperses body heat and creates a cooler sleeping surface while working with the core of pressure-relieving memory foam. Its cooling properties dissipate heat for a comfortable night’s sleep. The Memory Foam side responds to temperature and pressure to mold to your shape by creating the perfect support for your head, neck and shoulders.
The Right Pillow Matters
Choosing the right pillow is important. About 20% of your sleep surface is comprised of your pillow. You may not be sure of the right pillow, but you will definitely know when you have the wrong one. It is important to have a pillow which can contour to accommodate the natural curves of the neck to maintain the proper neck posture while sleeping. Whether you sleep on your side or back, your pillow must be soft enough to mold to your head and still fill in the space between your bed and your neck. You need to support both, since your head is larger than your neck and our gel memory foam pillow is great for this. This pillow will support the head at the proper height whether you are sleeping on your back or on your side, keep your head tilted in the proper position while sleeping on your back and provide neck and upper back support when sleeping on your back.
This pillow comes with a removable machine washable high-performance mesh knit cover.
Classic Brands Classic Brands Cool Reversible Gel and Memory Foam Pillow is backed with a 3-year worry-free warranty.
Look for more innovative specialty sleep products from Classic Brands
PharMeDoc Memory Foam Pillow with Cooling Gel Reversible Orthopedic Bed Pillow Includes Removable Pillow Cover, Standard Size
- Reversible bed pillow has two sides: cooling gel for warm nights and plush memory foam for cooler nights
- Thermoregulating Cooling Gel technology captures and diffuses heat for a cool, pleasant feeling throughout the night
- Soft memory foam provides head and neck support, helping align your spine, lessening painful pressure points, and retaining shape
- Removable pillow case makes cleaning quick and convenient while enduring 300+ washes
- Lifetime Manufacturer Warranty Designed in California 100% Satisfaction Guaranteed
Similar items with 4 stars and above
Chillow – Cooling Pillow for a Relaxing, Restful Sleep
- Easy to use cool pillow slips under your existing pillow for instant comfort
- Natural water cool gel pillow system provides the perfect cooling temperature
- Cooling pillow provides fast relief for flu, fever, headaches, hot flashes, sunburn, back pain and more
- Chillow is your own portable personal cooling system that goes anywhere
- Enjoy a relaxing cool bed for your head. Cradle yourself in comfort and relaxation
Forget tossing and turning to find the perfect cool spot for a restful nights sleep. The Chillow is a unique cooling pillow that provides soothing, cooling comfort all day or all night. Just add water and the one of a kind gel instantly dissipates heat. Slip it under your existing pillow for fast relief of pain, fever, hot flashes, sunburn and more. The cooling pads in the Chillow work to pull heat away from your body while providing the perfect temperature balance to help you enjoy a deeper, more relaxing and cooler sleep.
wavve Ventilated Gel Cooling Memory Foam Pillow for Sleeping Cool with Washable Zippered Cover, Standard Size
- ❅ SECOND GENERATION COOLING PAD: With high quality AirCell Technology memory foam, this pillow fits the curves and contours of your head and neck, and adjusts itself to your movements. It is made of high-density sponge with latest cooling pad, which is cooling and suitable for summer, making it more comfortable and smooth to touch.
- ❅ 3D MESH DESIGN: The mesh on the side offers more powerful ventilation and heat dissipation, which can speed up the heat flow inside the pillow and makes it cooler and more breathable, thus providing a better sleeping environment.
- ❅ TWO-SIDED FOR ALL SEASONS: The removable cover offers you easy cleaning. It is made of two different fabrics. The tencel side is ventilated and clean, offering softness and comfort. The ice fabric is breathable and skin-friendly, keeping you feel cold constantly.
- ❅ SUITS ALL KINDS OF SLEEPERS: Suits back, side and stomach sleepers, especially ideal for chronic pain sufferers, helping ease back and shoulder pain through all night while offering maximum comfort.
- ❅ SAFE & HEALTHY: In case you experience an odor which is a “Fresh Foam” odor, it is not harmful. The odor should dissipate several days later after the pillow is unpacked and aired out.
Better Sleep with wavve Gel Cooling Memory Foam Pillow!
Due to the perfect combination of memory foam and cooling gel, this pillow is perfect for getting the personalized support and comfort. The memory foam material is tested for quality, durability and content. The premium zippered cover is breathable, washable and durable, helping accelerates the airflow and allows you to sleep all night.
Weight: 4.2 lbs
You will receive a relatively small package, please unroll it and let it air out for 24-48 hours! In case you experience an odor which is a “Fresh Foam” odor, it is not harmful. The odor should dissipate within 30 minutes from when the foam is unpacked and aired out.
Store in a Cool and Dry Area Direct away from Sunlight.
How to maintain:
1. Filling: use a vacuum cleaner or just pat it gently to clean. Please immediately dry the water if accidental wet happens. DO NOT wash the filling with wash machine.
2. Pillow case: hand & machine washable. Air-dry.
100% Satisfaction Guarantee:
If you are not satisfied with our memory foam pillow, please contact with us by email and we are available to answer your questions 24/7.
My Pillow Classic Series [Std/Queen, Firm Fill] Now Available in 4 Loft Levels
- NEW SLEEP STUDY PROVEN – 100% of sleep study participants increased their amount of deep sleep with MyPillow!
- 96% of Participants Experienced Less Sleep Interruptions
- Helps Reduce Snoring – Reduces Daytime Fatigue – Reduces Sleep Interruptions – Helps Improve the Quality of Your Sleep
- Patented Adjustable Interlocking Fill – Will Not Go Flat [10-Year Warranty] Machine Washable & Dryable
- Made in the USA – Manufactured in our home state of Minnesota! – 18.5” x 28”
This classic MyPillow features a medium fill design and is sure to have you sleeping comfortably.
Coop Home Goods – Eden Shredded Memory Foam Pillow with Cooling Zippered Cover and Adjustable Hypoallergenic Gel Infused Memory Foam Fill – Standard
- Inner Cover – 100% Polyester Outer Cover – 40% Bamboo Derived Rayon 60% Polyester
- Made in USA
- WAKE UP REFRESHED – Our Eden pillow provides better neck support while you sleep. Our gusseted design prevents pillow pinch (when the edges of your pillow slope together and fill gets pushed out from under your neck). With the Eden pillow, you have maximum pillow support, allowing for edge-to-edge support and helping you get the refreshing rest you need.
- NO MORE SWEATY BED HEAD – Our Ultra-Tech fabric made of 40% bamboo-derived viscose rayon and 60% polyester provides breathability, while the cooling gel memory foam helps draw out heat and keep you at a comfortable temperature throughout the night. Because our Eden memory foam is shredded into cross-cut pieces and includes poly fibers to prevent it from clumping together or getting too compact, which means more airflow and you get to a comfortable temperature and undisturbed sleep
- DON’T ADJUST TO YOUR PILLOW – YOUR PILLOW SHOULD ADJUST TO YOU – Featuring our patent-pending adjustable design, the Eden pillow can be adjusted by you at home and puts its customization in your control
- you don’t need to send your pillow to the manufacturer and wait for them to adjust it. Our Eden pillow features a zippered inner and outer cover, allowing you access to its proprietary fill and the ability to add or remove as much of it as you need to meet your own unique needs.
- SLEEP SMART – You spend the night on your pillow, so it only makes sense to know what you’re sleeping on. Our pillows are made in the U.S.A., contain CertiPUR-US certified memory foam, are made without PBDEs, TDCPP, or TCEP (“Tris”) flame retardants, and are hypo-allergenic and dust mite resistant. They feature a removable outer cover for easy cleaning and the pillow itself is also machine-washable.
- BACKED BY THE COOP HOME GOODS GUARANTEE – We guarantee you’ll love the pillow, and if you aren’t absolutely satisfied, return it within 100 days for a refund, no questions asked.
Pillows for Sleeping, Hypoallergenic Bed Pillow for Side Sleeper – ADJUSTABLE Loft Bamboo Memory Foam Pillow – Kool-Flow Micro-Vented Bamboo Cover, Washable – Premium – MADE IN THE USA – QUEEN
- MADE IN THE USA – FULLY ADJUSTABLE – IDEAL FOR ALL SLEEP POSITIONS. BACK, SIDE, STOMACH. The NEW inner zipper allows you to ADJUST THE PILLOW THICKNESS to make it a perfect fit for YOU. – Whether you like it fluffy, flat or in the middle, simply open the zipper &
- remove some fill to have it your way. – FALL ASLEEP FAST and SLEEP BETTER than you ever have before! “THE BEST BED PILLOW” ★ RATED #1 by THE WIRECUTTER an independent review organization – DON”T BE FOOLED by Cheaper Imitations.
- MICRO-VENTED KOOL-FLOW TECHNOLOGY BAMBOO COVER is soft as a cloud &
- provides superior air circulation and breathability while regulating temperature to KEEP THE PILLOW SURFACE COOL for the best sleep ever! No more sweaty faces – Experience extreme comfort with our memory foam pillow
- OUR MEMORY FOAM PILLOWS USE ONLY CertiPUR-US CERTIFIED FOAM &
- MEET STRINGENT STANDARDS for content, emissions &
- durability. They are analyzed by independent, accredited testing labs. – SLEEP RESTFULLY knowing that our pillows for sleeping have NO ozone depleters, TCEP flame retardants, TDCPP, PBDEs, lead, mercury, and heavy metals, formaldehyde, phthalates regulated by the CPSC, or CFCs – This assures you and your family are using a HEALTHY AND SAFE PRODUCT especially for those during pregnancy.
- ULTRA DELUXE PREMIUM QUALITY SHREDDED MEMORY FOAM will not become lumpy or go flat. It remains uniform in density, CONFORMING TO YOUR PERSONAL HEAD &
- NECK CONTOUR for a perfect nights sleep &
- returns to shape, night after night, – Because the foam is shredded, you can maneuver &
- mold your pillow so that it is comfortable for back stomach &
- side sleepers. – Promotes PROPER BACK, NECK &
- SPINE ALIGNMENT. Help BACK &
- NECK PAIN, TOSSING &
- TURNING, Insomnia, Migraines, Snoring and ORTHOPEDIC PROBLEMS.
- HYPOALLERGENIC – DUST MITE RESISTANT – MACHINE WASHABLE – Allergy sufferers no longer need to compromise comfort! Now you can have a memory foam pillow that is clean all the way through! – They ship vacuum sealed &
- rolled for frustration free Eco-friendly shipping. – YOU &
- YOUR FAMILY DESERVE THE BEST BED PILLOWS! – Our pillows are BUILT FOR COMFORT, SUPPORT &
- DURABILITY! – MONEY BACK GUARANTEE. If you aren’t 100% satisfied, simply return it within 30 days! Best in the Business 6 year Warranty!
LIANLAM Memory Foam Pillow for Sleeping Shredded Bed Bamboo Cooling Pillow with Adjustable Loft 4D Design Hypoallergenic Washable Removable Derived Rayon Zip Cove (Standard)
- 40% Rayon/60% Polyester
- ????PERSONALIZE TO YOUR SLEEP POSITION – With our premium adjustable pillow design,the shredded memory foam pillow can be adjusted very easily by you at home.With LIANLAM Pillow featured zippered inner and outer cover, you can take control of your sleep by adjusting to the ideal pillow height for your sleep position by adding or removing foam fillings.We also added extra memory foam, just in case you really like a fluffy pillow.
- ????STAY COOL ALL NIGHT (GEL INFUSED) – Are you tired of waking up in the middle of the night with sweaty bed head?Our high tech fabric is made up of 40% bamboo-derived viscose rayon and 60% polyester to ensure extreme breathability, while the cooling gel memory foam helps draw out head and keep a comfortable temperature throughout the night. Not to mention our memory foam cross-cut design prevents clumping and avoids causing a compact air restricted feeling, giving you an ultimate quiet night
- ????FOR ALL SLEEPERS (SIDE/STOMACH/BACK) – With our adjustable memory foam pillow and extra foam,you can rest assured you will be satisfied. Side sleepers, back sleepers, and stomach sleepers can all benefit from the ergonomics of our customizable bed pillow, allowing edge-to-edge support, which can help reduce neck and shoulder pain, while ensuring maximum comfort and truly providing the refreshing rest that we all desperately need.
- ????HIGHEST QUALITY CERTIFICATION – Our pillow is made WITHOUT any harmful material, such as ozone depleters, PBDE flame retardants, mercury, lead, formaldehyde and is certified to the highest consumer quality and safety standards by CertiPUR-US. Our outer bamboo pillow cover is breathable, hypoallergenic, odor-free and machine washable in cold water. Our premium inner and outer covers have the highest quality zippers for easy cleaning!
Similar items with 4 stars and above
Leesa Luxury Hybrid Reversible Cooling Foam/Quilted Pillow for Sleeping, Standard, White
- DESIGNED TO BE COOL: Our pillow fabric is made with fibers that give a chilling effect and a ventilated gel comfort layer for a cooler night’s sleep.
- DIFFERENCE IN THE DETAILS: This pillow has a smooth zipper, a seam that goes unnoticed through a pillowcase and quilted zones of down-like filling that stay put.
- CUSTOMIZED COMFORT: No matter how you choose to customize it, the pillow is designed for consistent support and made for your mind to drift into slumber.
- SLEEP EASY: Because we know it takes more than one night to fall in love, we want to make your purchase 100% risk-free. All Leesa mattresses and pillows include a 100-night in-home trial backed by a no-hassle, full refund policy.
- OUR SOCIAL IMPACT: We set out to create a company with heart—we measure our success as much by our bottom line as by our impact on the world. We donate one mattress for every 10 we sell (pillows, too), plant one tree for every mattress we sell and volunteer in our local communities.
Our adjustable and reversible hybrid pillow features a down-like quilted pocket side and a cooling side for a restorative sleep experience.
Similar items with 4 stars and above
By our suggestions above, we hope that you can found chillow cooling pillow for you.Please don’t forget to share your experience by comment in this post. Thank you!
Best chillow cooling pillow: Top-Rated Brands & What to Buy


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A micro red-dot mounted atop a handgun can be a very useful little tool, but not all optics and optics-ready pistols play well together, creating a potentially confusing situation for prospective buyers. With this resource, we attempt to clear the air.
On this week’s “Gun of the Week” video preview American Rifleman staff have the latest Optics Tactical version of EAA’s Churchill 620 series of manually operated shotgun in 20 gauge.
On this week’s “Gun of the Week” video preview, American Rifleman staff are checking out an optic-ready, hybrid version of the 1911 pistol.
Even just a few years ago, optics-ready and micro-compact pistols were the exception. Now, they're starting to look like the rule.

domain: hobby


text:
Please contact us if you would like this design and we can create a similar alternative.
The moment you lay eyes on Audrey you’re in love. She is the star attraction in our studio and you simply can’t resist the urge to touch. She’s filled to the brim with our most exquisite stems and evokes memories of English garden parties in the height of summer. Her soft, neutral tones makes her timeless and elegant with a dash of whimsical. Front facing arrangement. Please note, we are now using green berries rather than the cream berries pictured.
Audrey is arranged in our tapered glass vase which has a base diameter of approx. 15cm and overall measures approx 70cm high and 50cm wide. She is prepared to order, beautifully presented with handwritten stationery and of course our signature Secret Blooms wooden seal of authenticity. Whilst every effort is made to illustrate the product’s true likeness in our photos, there is likely to be some minor variation in the appearance and / or size of your product as a result of our arrangements being prepared to order.

domain: design


text: Today, the Invasion event officially ended. With this, the official Valve servers for the event also shut down, removing all chance of playing the officialized Invasion maps on official Valve servers. When the Gun Mettle campaign ended, the maps that were added to the game were added to the standard Valve rotation on Valve servers. In other words, the servers would randomly roll on to them, and can be voted for them to be set as the next map. However, for Invasion maps, none of the maps are available to vote as the next map on Valve servers currently. Gun Mettle maps are still there, however. There's roughly 20-ish servers currently running arena_byre, pd_watergate and koth_probed, and another 20 running ctf_2fort_invasion. As it stands, those servers are the only way to play Invasion maps currently. While understandably arena_byre is a bit of an oddball to fit on to any of the current server rotations, what with there being no Arena servers, koth_probed can easily fit in the KOTH rotation, and pd_watergate could either go in KOTH or CTF/SD. ctf_2fort_invasion could also fit in the CTF section, but might be best left to throw in to the Halloween rotation next year. I hope Valve considers adding some of those maps to the main rotation. Otherwise they're doomed to die a wet, hydroelectric death.


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Wednesday, July 7, 2010
Good Lord! It's good to be back!
It's been a while since I have the time to write (properly). My "holiday from doing any act of writing" just turns out to be an irony. It's weird that in my busiest time of the day, I have always been catching myself off-guard, scribbling my thoughts in my assignment papers/exam papers/bedroom... Sign in
to see full entry.

domain: literature


In case you missed our live remote from La Fete de Marquette on Friday July 15 (or you just want to hear it again or share it with someone else,) here are the full sets from New Orleans bands Sweet Crude and Bonerama!
WORT is broadcasting live from La Fete de Marquette this Friday night, July 15th. Tune in to our special audio remote for a pair of eclectic bands from New Orleans. Laissez les bon temps roulez, eh? At 7:15 p.m. Friday WORT will interrupt the Blues Cruise in order to present a live performance by bilingual […]


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Apple is all set to make some radical changes in its upcoming iPhones that will come in second half of this year. The major shift will be in dimensions as screen size can be as large as 5-inches. And as for the iPhone 5C, it is to be scrapped soon.
The factories at Apple are ready to churn out some new stuff that will resonate with the technology-loving crowd. First off, the size of its new iPhone 6 range is to be larger than the current lot. The two novel smart phones are to be 4.5 inches diagonally and 5 inches diagonally respectively.
That is a huge difference and will take some adapting to. As for the casing, it is not going to be plastic-based like the one on the iPhone 5C. Rather the models for the future will imitate the iPhone 5S with its shining gold and silver metallic casing. Furthermore, the smartphones of the times to come will not be curved.
The iPhone 5C meanwhile is headed for the dustbin of history. It is a project that will be put on hold indefinitely. Besides the bigger screens and metallic casings, the fresh gadgets from Apple will feature a ton of other quality integrated hardware and software. The large screens will be particularly popular in China which is a huge potential market.
These changes made by Apple came about after some honest and well-thought calculations were made by the experts in their labs. iPhone demands have surged too by 10% making the smart phone deal a lucrative one for Apple. The times ahead look good for Apple provided it hears the voice of its audience (which is the world).
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domain: news


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ORIGINAL RESEARCH article
Transcranial Direct Current Stimulation Ameliorates Cognitive Impairment via Modulating Oxidative Stress, Inflammation, and Autophagy in a Rat Model of Vascular Dementia
- 1Department of Emergency, The Second Xiangya Hospital, Central South University, Changsha, China
- 2Department of Neurology, The Second Xiangya Hospital, Central South University, Changsha, China
- 3Department of Pathology, The Second Xiangya Hospital, Central South University, Changsha, China
- 4Department of Oncology, The Second Xiangya Hospital, Central South University, Changsha, China
To investigate the potential applications and the molecular mechanisms of transcranial direct current stimulation (tDCS) on cognitive impairment in a vascular dementia (VD) animal model. Sprague-Dawley rats were used in this study. VD rat model was induced by modified permanent bilateral common carotid artery occlusion (2-VO) approach. Anodal tDCS was applied to the animals. Morris water maze was used to analyze spatial memory and navigation ability. The pathological changes in the hippocampal CA1 region and cerebral cortex were examined via Hematoxylin-Eosin staining. The rats were sacrificed for the measurement of the level of superoxide (SOD), glutathione (GSH), reactive oxidative species (ROS), malondialdehyd (MDA), Interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α level in the hippocampus. Western blot was carried out to measure the hippocampal expression of microtubule-associated protein 1 light chain 3 (LC-3) and p62. Rats with VD have decreased number of neurons in the hippocampus and cerebral cortex, as well as worse cognitive impairment. The proliferation of activated microglia and astroglia, accompanied with attenuation of myelination were observed in the white matter about 1 month after 2-VO operation. These abnormalities were significantly ameliorated by tDCS treatment. Further study revealed that anodal tDCS could suppress the MDA and ROS level, while enhance the SOD and GSH level to reduce the oxidative stress. Anodal tDCS could inhibit hypoperfusion-induced IL-1β, IL-6, and TNF-α expression to attenuate inflammatory response in hippocampus. Moreover, anodal tDCS treatment could alleviate autophagy level. The study has demonstrated a possible therapeutic role of tDCS in the treatment of cognitive impairment in VD.
Vascular dementia (VD) is the second most common cause of dementia after Alzheimer’s disease, accounting for around 15% of cases (O’Brien and Thomas, 2015). Rates of VD rise with age. There have been no effective approved pharmacological treatments available for VD up till now. Chronic cerebral hypoperfusion played a causative role in VD (Du et al., 2017). Previous studies demonstrated that cerebral hypoperfusion could lead to oxidative stress, neuroinflammation, neurotransmitter system dysfunction, mitochondrial dysfunction, disturbance of lipid metabolism, and alterations of growth factor (Du et al., 2017). Oxidative stress plays an important role in cognitive deficits induced by the chronic cerebral hypoperfusion (Chunjiea et al., 2016). Neuroinflammation characterized by Interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α plays an important role in VD (Belkhelfa et al., 2018). Autophagy, a lysosome-mediated catabolic pathway, contributes to the maintenance of cellular homeostasis (Moloudizargari et al., 2017). Decreasing autophagic activity may contribute to cognitive improvement in rats with VD (Liu et al., 2018; Venkat et al., 2018).
Transcranial direct current stimulation (tDCS) is a non-invasive neuromodulation technique that has been used to modulate brain function (Paulus, 2011). Anodal tDCS could induce long-lasting alterations of cortical excitability and enhance cerebral plasticity, both in experimental animals and humans (Datta et al., 2010). Anodal tDCS could affect synaptic plasticity, modulate the level of oxidative stress, neuroinflammation, and autophagy (Scelzo et al., 2011; Laste et al., 2012; Lu et al., 2015; Lee et al., 2018). Indeed, anodal tDCS is a promising approach for brain diseases associated with impaired neuroplasticity, which is simple to be used and is beneficial for brain function (Podda et al., 2016). It’s speculated that anodal tDCS might exert similar roles in alleviate cognitive impairment in VD. Anodal tDCS has been proven to be beneficial for cognitive function in patients with VD (André et al., 2016). However, the studies of tDCS on VD animal model are limited.
In the current study, we have tried to investigate the possible roles and mechanisms of tDCS on improvement of cognitive impairment of VD rat model. The study could provide some evidences for using anodal tDCS as a potential non-pharmacological treatment for VD.
Materials and Methods
Animal use protocols were approved by the Institutional Animal Care and Use Committee of Central South University in compliance with National Institutes of Health guidelines. Sixty male Sprague-Dawley rats, weighting 250–280 g (6–8 weeks old), were provided by department of laboratory animals in Central South University. The rats were maintained under controlled temperature and humidity (22 ± 3°C and 50%, respectively) with a 12 h light–dark cycle. All efforts were made to limit the number of rats used and to minimize animal suffering. All food and water were provided ad libitum throughout the trial. To generate a rat model of VD, permanent bilateral common carotid artery occlusion (2-VO) approach was applied as previously reported (Zhu et al., 2011). Briefly, rats were anesthetized with chloral hydrate; a neck ventral midline incision was made. The common carotid arteries were exposed and then gently separated from the vagus nerve. Carotids were occluded with a 1-week interval between interventions, the right common carotid being the first to be processed and the left one being occluded 1 week later (Cechetti et al., 2010; Mirzapour et al., 2015). The sham operated rats underwent the same procedures without carotid artery ligation. After surgery, rats were left to recover for a period of 1 week. Sixty SD rats were randomly divided into three groups: (1) Sham group: Sham operation group treated with sham stimulation, (2) VD group: VD rat models treated with sham stimulation, (3) tDCS group: VD rat models treated with anodal tDCS. Figure 1 summarized the temporal evolution of the study protocols. Rats that exhibited abnormal behavioral effects during the study, such as seizures, were excluded to avoid any potential impact on the final results.
One week after surgery, rats were placed in a stereotactic frame after anethetized with chloral hydrate (350 mg/kg). A sagittal incision was made in the scalp. A scalp and the underlying tissues were removed, then the skull was dried with cotton swabs. A custom-made polycarbonate tubes with the inner diameter of 1 mm and the contact area on the skull of 3.14 mm2 were stereotactically placed on the sagittal suture with the center of the electrode resting on 2.5 mm posterior to bregma, then the tubes were subsequently attached to the bone surface with a thin layer of non-toxic dental cement (super-bond C&B, Sun Medical, Japan) and a second layer of two-component luting resin (Ketac Cem Plus, 3MESPE AG, Germany) (Pikhovych et al., 2016; Yu et al., 2019). To ensure current flow during stimulation, the hollow implant was kept free of cement. After surgery, the rats were transferred back to their home cages and were allowed to recover for 1 week before undergoing tDCS. After at least 1 week of post-surgical recovery, rats were randomized into two groups receiving anodal tDCS or sham stimulation, respectively. All procedures of tDCS and sham stimulation had identical duration of current fade-in (10 s), fade-out (10 s) and current strength (200 μA), except the stimulation duration (tDCS 30 min, sham 10 s) (Yang et al., 2019; Yu et al., 2019). Anodal tDCS was repeated daily for 5 consecutive days, followed by a 2-day pause, then subjected to another set of 5 stimulation days, resulting in a total of 10 days of tDCS stimulation (Rueger et al., 2012; Pikhovych et al., 2016; Yang et al., 2019). The stimulation was conducted by the same researcher at the same time every day. The anodal electrode was inserted into the polycarbonate tube, which was filled with saturated saline. To avoid debris accumulating in the polycarbonate tube, a cotton ball was placed to seal the tube when not in use (Pikhovych et al., 2016). The cathode electrode was a conventional rubber-plate electrode wrapped by a wet cotton sheath (11 cm2) applied over the ventral thorax of the rat by an elastic bandage (Podda et al., 2016). Both anodal and cathodal electrodes were connected to a direct current stimulator (Ruihaikanglian, Jiangsu, China) for electric current stimulation.
Morris Water Maze Task
The cognitive function was analyzed by the Morris water maze (MWM) test 24 h after the last tDCS stimulation according to previously described protocols (Guo et al., 2017; Schoenfeld et al., 2017). Briefly, a circular water tank with 150 cm diameter and 50 cm depth was filled with 25°C water to a depth of 21 cm. A circular platform (diameter: 10 cm; height: 20 cm) was located in the center of the target quadrant. The navigation trials were conducted for 5 consecutive days. The time taken for the rats to find the platform was recorded as the escape latency. When the rats reached the platform within 90 s, they remained on the platform for 20 s. If the rats failed to find the platform within 90 s, they were gently guided to the platform and left on it for 20 s. The escape latency was recorded as 90 s in such cases. One day after the navigation trial, the platform was removed for a probe trial. The time spent in the target quandrant and the numbers of swimming across the platform site for up to 90 s were recorded using a computer-based image analyzer automatically.
The brain tissues from rats were collected after completion of the tDCS treatment. All rats were deeply anesthetized using 10% chloral hydrate (400 mg/kg). A subset of rats were perfused transcardially with 0.9% NaCl followed by 4% paraformaldehyde in 0.1M phosphate-buffered saline (PBS). The brains of these rats were removed and post-fixed in the same fixative at 4°C overnight. Then they were immersed consecutively in 20 and 30% sucrose at 4°C until they sank. The remaining post-fixed brains from 2.15 to 5.76 mm behind bregma were embedded in paraffin and then cut into 10 μm thick coronal sections. Hematoxylin and eosin (HE) staining were used to observe any histological changes. Fresh brain tissues were quickly taken and then was fixed in 4% paraformaldehyde for immunocytochemistry or stored at −80°C for enzyme-linked immunoabsorbent assay (ELISA) and western blot.
Hematoxylin and eosin staining was used to evaluate the morphological changes of the hippocampal region CA1 and cerebral cortex. Sections were then sequentially immersed in hematoxylin for 10 min and in eosin for 1 min (Fan et al., 2018). Four random visual fields from each brain slice were analyzed. Morphological changes of neurons in the hippocampus and cerebral cortex were observed under a microscope at 100 and 400× magnification respectively. The number of surviving neurons in the hippocampal CA1 area and cerebral cortex was quantified under a light microscope at 400× magnification through Image-Pro Plus software.
The coronal sections were incubated overnight with anti-glial fibrillary acidic protein (GFAP) antibody, anti-ionized calcium binding adaptor molecule 1 (Iba1) antibody, and anti-myelin basic protein (MBP) antibody. After they were washed, the sections were treated with appropriate biotinylated secondary antibodies. To stain cell nuclei, the sections were incubated with 4′-6-diamidino-2-phenylindole (DAPI) in PBST for 30 min. These sections were visualized by the diaminobenzidine tetrahydrochloride (DAB) and H2O2. The white matter lesions, astroglia and microglia activations were analyzed in corpus callosum and internal capsule. Quantitative analysis of GFAP, Iba1 or MBP-positive cells present in the sections was carried out under 400 × microscopic magnification by Image-pro Plus software respectively. At least three random microscopic fields from each section (three sections per rat) were calculated. The results were presented in the tiled images. All counts were performed in a blinded fashion.
Measurement of ROS
The intracellular levels of reactive oxidative species (ROS) were assessed by the probe of DCFH-DA. The hippocampus was separated, rinsed twice with PBS, made into cell suspensions, then incubated in trypsin at 37°C for 25 min. Add ice-cold PBS to stop the reaction. Cells were collected and centrifugated. The sediment was incubated with DCFH-DA containing PBS at 37°C for 30 min. The fluorescene-oxidized deprivation of DCFH-DA was minitored with fluorescene microplate and ROS levels were measured (Jiang et al., 2017).
Enzyme-Linked Immunoabsorbent Assay
The hippocampus was taken out and put into the homogenate tube after being washed by cold normal saline. Appropriate amount of normal saline at 4°C was added into the tube. Homogenized the tissue, centrifugated at 4000 × g for 15 min, then collected the supernatant. The oxidative stress markers in hippocampus were measured: superoxide dismutase (SOD), malonic dialdehyde (MDA), and glutathione (GSH) (Jiang et al., 2017; Al-Amin et al., 2018). The protein levels of interleukin (IL)-1β, IL-6 and TNF-α were evaluated by specific ELISA kits following manufacturer’s instructions (Akang et al., 2019). The commercial kits for examining these parameters were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
The expression levels of autophagy markers in the hippocampus were analyzed by Western blot. In brief, hippocampuses were washed with ice-cold PBS and homogenized in ice-cold RIPA lysis buffer. Total protein concentration was quantified by BCA kit. About 20–40 μg protein samples were loaded onto SDS-PAGE gel for electrophoresis separation and were transferred to PVDF membrane. After blocking, the membranes were incubated overnight at 4°C with primary antibodies. The membrane was then developed by ECL substrate, and images were captured by a computerized system. Quantification was performed using Image J software. The following primary antibodies were used: microtubule-associated protein 1 light chain 3 (LC3) and p62. Insoluble p62 cannot be extracted by conventional methods, so soluble p62 was studied in the present study.
All statistical analysis was performed using SPSS 22.0 software. The graphs were prepared by GraphPad prism (version 6.0, GraphPad Software, Inc.). Multiple comparisons were analyzed by one-way ANOVA with post hoc Bonferroni test. All data were presented as mean ± standard deviation (SD). P < 0.05 was considered as statistically significant.
Effects of tDCS on Hypoperfusion-Induced Cognitive Impairment
In this study, 60 male SD rats were used. Two rats died during the 2-VO surgery. The remaining rats completed all the experiments without any abnormal behavioral effects, such as seizures. All rats successfully finished the task with decreasing latencies to reach the platform. The latencies to find the hidden platform and the swimming distances of the VD rats were significantly longer than those of the rats in the sham group (P < 0.05). Furthermore, tDCS induced a significant decrease in escape latencies and swimming distances across 5 days’ MWM training compared with the rats in the VD group (P < 0.05) (Figures 2A,B). In the spatial probe trials, rats in the VD model group made significantly fewer crossings of the platform area compared to those in the sham group (P < 0.05), whereas rats in the tDCS group crossed the platform area significantly more times than those in the VD group (P < 0.05) (Figure 2C). Rats in the VD group spent less time in the target quadrant compared with the rats in the sham group, while tDCS improved the reduction (P < 0.05) (Figure 2D). The typical swimming paths recorded in the spatial probe trial of each group were displayed in Figure 2E.
Figure 2. Transcranial direct current stimulation (tDCS) attenuated learning and memory deficits induced by chronic hypoperfusion. (A) Escape latency. (B) Swimming distance. (C) Number of crossings on the platform. (D) The time spent in the target quadrant in the spatial probe trials. (E) Typical swimming traces of all groups in the spatial probe trials (compared to Sham group, *P < 0.05; compared to VD group, △P < 0.05).
Effects of tDCS on Neurons in Hippocampus and Cerebral Cortex
The representative photomicrographs of neurons in hippocampal CA1 region and cerebral cortex were shown in Figure 3. Neurons exhibited regular and compact arrangement in the hippocampal CA1 region and cerebral cortex of sham group rats, with the cytoplasm stained and well-distributed. In the VD group, neuron loss, shrinkage and loose arrangement were observed in the hippocampal CA1 region and cerebral cortex. While in the tDCS group, the neurons demonstrated a nearly normal appearance in the hippocampal CA1 region and cerebral cortex, comparable to those in the sham group. The number of live neurons significantly decreased in the hippocampal CA1 region and cerebral cortex of the VD group than the sham group, while a higher count of live neurons was found in the hippocampal CA1 region and cerebral cortex of tDCS group than the VD group (P < 0.05) (Figure 3).
Figure 3. (A) Morphological changes in the hippocampal CA1 area and cerebral cortex of rats in each group (HE staining) (×400); Quantification analysis of the numbers of live neurons in the hippocampus (B) and the cortex (C) of rats from each group (*P < 0.05).
Effects of tDCS on Demyelination and Glial Activation
In the corpus callosum and internal capsule, a significant increase in microglia and astroglia was observed in rats in the VD group compared with rats in the sham group, accompanied by a greater loss of white matter myelin in rats in the VD group. Anodal tDCS attenuated demyelination and glial activation in rats with VD (Figures 4, 5).
Figure 4. (A) Photomicrographs of immunohistochemistry staining for GFAP, Iba1, and MBP in the corpus callosum of rats in each group (×400), scale bar: 100 μm. Quantification analysis of the number of GFAP positive (B), Iba1 positive (C), and MBP (D) positive neurons in the corpus callosum of rats from each group (n = 6 per group, three sections per rat) (*P < 0.05).
Figure 5. (A) Photomicrographs of immunohistochemistry staining for GFAP, Iba1, and MBP in the internal capsule of rats in each group (×400), scale bar: 100 μm. Quantification analysis of the number of GFAP positive (B), Iba1 positive (C), and MBP positive (D) neurons in the internal capsule of rats from each group (n = 6 per group, three sections per rat) (*P < 0.05).
Effects of tDCS on Oxidative Stress Level
By using commercial kits, we found that SOD (P < 0.05) and GSH (P < 0.05) reduced significantly, while the levels of ROS (P < 0.05) and MDA (P < 0.05) increased significantly in the hippocampus of rats with VD. Treatment with tDCS significantly increased the SOD and GSH content. After tDCS treatment, the level of ROS and MDA were suppressed. Thus, tDCS could effectively inhibit the oxidative stress in rats with VD (Figure 6).
Figure 6. Effects of tDCS on the levels of SOD (A), GSH (B), MDA (C), and ROS (D) in the hippocampus of rats from each group (*P < 0.05).
Effects of tDCS on Inflammatory Parameters
By ELISA quantification, we found that the VD rats had remarkably increased levels of inflammatory factors such as IL-1β, IL-6, and TNF-α (P < 0.05). Anodal tDCS significantly alleviated hippocampal protein levels of IL-1β, IL-6, and TNF-α (P < 0.05) in the rat model of VD (Figure 7).
Figure 7. Effects of tDCS on the levels of IL-1β (A), IL-6 (B), and TNF-α (C) in the hippocampus of rats from each group (*P < 0.05).
Effects of tDCS on Autophagy Level
To determine the effects of tDCS on autophagy, the ratio of LC3-II/LC3-I and the protein expression level of soluble p62 in the hippocampus were analyzed. The ratio of LC3-II/LC3-I was significantly greater in the VD group than that in the sham group (P < 0.05). In addition, the expression level of the soluble p62 protein was markedly decreased in the VD group compared with that in the sham group (P < 0.05). Anodal tDCS attenuated these changes (Figure 8).
Figure 8. Effects of tDCS on the levels of LC3-II/LC3-I (A) and soluble p62 (B) in the hippocampus of rats from each group (*P < 0.05).
Anodal tDCS has promising potential therapeutic effects for symptoms associated with dementia (Elder and Taylor, 2014). Anodal tDCS exerts positive effects on cognition and brain functions in mild cognitive impairment and major dementias, including Alzheimer’s disease, Pakinson’s disease, however, available studies on VD were limited (Meinzer et al., 2015; Adenzato et al., 2019; Gomes et al., 2019).
Chronic cerebral hypoperfusion is closely related to progressive cognitive impairment in rats (Lin et al., 2011). The most widely used experimental model of VD and chronic cerebral hypoperfusion is 2-VO rats (Du et al., 2017). It’s reported that the modified 2-VO protocol may be more applicable, with similar cognitive impairment and lower mortality rates, compared to the standard 2-VO procedure (Cechetti et al., 2010). The present study established a rat model to reproduce chronic cerebral hypoperfusion by modified 2-VO. Cerebrovascular white matter lesions are caused by chronic cerebral hypoperfusion in VD. The neuropathological changes in VD rat models were characterized by diffuse demyelination and gliosis in the white matter, accompanied with neurodegeneration in the hippocampus and cerebral cortex, which were in accordance with previous reports (Shibata et al., 2004). Hippocampus was sensitive to ischemia, especially the hippocampal CA1 region (Ohtaki et al., 2003).
Brain stimulation techniques can attenuate cognitive impairment in many neuropsychiatric diseases (Chang et al., 2018). The anodal tDCS has been conducted in healthy subjects, Parkinson’s disease, Alzheimer’s disease, multiple sclerosis, depression, and attention disorders, etc. (Lefaucheur, 2016; Chang et al., 2018; Charvet et al., 2018; Manenti et al., 2018). However, studies investigating the effect of tDCS on VD were limited. It’s reported that anodal tDCS of the left dorsolateral prefrontal cortex could improve visual short-term memory in patients with VD (André et al., 2016). The present study demonstrated the beneficial effects of anodal tDCS on the cognitive deficit in a VD rat model. Our study is new in the field of tDCS treatment by demonstrating its anti-autophagy, anti-inflammatory, and anti-oxidant effects in VD.
Autophagy is a self-degradative process that is important for balancing sources of energy. It involves lysosomal-dependent recycling, synthesis, and degradation of intracellular components, thus maintaining the stability of the internal environment. It’s reported that decreasing activity of autophagy may contribute to cognitive improvement in rats with VD (Venkat et al., 2018; Wang et al., 2018). Inhibition of autophagy was beneficial to the hippocampal synaptic plasticity of the VD rat model (Bin et al., 2019). In the present study, the autophagy-lysosomal pathway was activated in the hippocampus of rats with VD. Anodal tDCS could restore the excessive activation of autophagy and help to partly recover the lost learning and memory in VD rats. It’s reported that BDNF expression was enhanced by anodal tDCS, and BDNF could modulate autophagy through the PI3K/Akt/mTOR pathway (Chen et al., 2013). At present, role of autophagy activation in ischemic stroke remain controversial. Autophagy is a double-edged sword. Excessive autophagy can induce autophagic cell death. On the other hand, moderate activation may play a protective role in cell damage.
Increased production of ROS or decreased capacity to clear them could result in oxidative stress. Our data revealed a decrease in the activities of antioxidant enzyme (SOD and GSH) in VD rats, which was attenuated by tDCS. The level of MDA and ROS was reduced by tDCS. In this study, anodal tDCS suppressed oxidative stress induced by chronic cerebral hypoperfusion and protected the hippocampal neurons from further damage induced by overload of ROS. It’s reported that tDCS could reduce oxidative stress in a mouse model of Parkinson’s disease as well (Chengbiao et al., 2015). However, how the altered oxidative status caused by tDCS remained to be determined in the future study.
Neuroinflammation plays an important role in VD. Proinflammatory cytokines IL-1β, IL-6, and TNF-α independently resulted in cognitive impairment (Dugan et al., 2009; Kitazawa et al., 2011; Belarbi et al., 2012). Our data demonstrated that anodal tDCS could significantly restore hippocampus-dependent cognitive deficit induced by neuroinflammation. Previous studies have revealed a decrease in IL-1β and TNF-α after a treatment with tDCS (Leffa et al., 2018; Oliveira et al., 2019). However, how tDCS modulate neuroinflammatory pathways is still not completely understood.
There were some limitations in the present study. First, rats were sacrificed immediately after the last tDCS stimulation. Longer time interval and different time points should be adopted to evaluate the persistence of the effects of tDCS. Second, VD is reported clinically in both males and females. Only male rats were used in the study, therefore a further study is needed to include both male and female rats. What’s more, 2-VO rat model is a VD rat model induced by chronic cerebral hypoperfusion. Rats performed MWM test only about 1 month after 2-VO surgery in the study. Longer time intervals should be adopted to reveal whether the improvements were permanent or not, such as 2 or 3 months after 2-VO surgery.
In summary, our data confirmed that anodal tDCS exerted a neuroprotective effect in the 2-VO rat model. The mechanisms by which anodal tDCS exerted its neuroprotective effects likely involved the modulation of oxidative stress, neuroinflammation, and autophagy. The present study suggested a theoretical basis for the use of anodal tDCS as a potential neuroprotective therapy to improve cognitive impairment of VD.
Data Availability Statement
All datasets generated for this study are included in the article/supplementary material.
All animal procedures were approved by the Ethics Committee of the Central South University. This study was carried out in strict accordance with the Institutional Animal care and Use Committee of Central South University in compliance with NIH guidelines.
JF conceived and designed the experiments. JF, ZT, and SH performed the experiments. JF, TG, ZT, SH, and YL analyzed the data, contributed data, materials, and analysis tools. JF and TG wrote the manuscript.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
This work was supported by Natural Science Foundation of Hunan Province (No. 2018JJ3726).
Adenzato, M., Manenti, R., Enrici, I., Gobbi, E., Brambilla, M., Alberici, A., et al. (2019). Transcranial direct current stimulation enhances theory of mind in Parkinson’s disease patients with mild cognitive impairment: a randomized, double-blind, sham-controlled study. Transl. Neurodegener. 8:1. doi: 10.1186/s40035-40018-40141-40039
Akang, E. N., Dosumu, O. O., Olasunmbo, O., Afolayana, A., Fagoroye, D. M., Osiagwu, D. D., et al. (2019). Combination antiretroviral therapy (cART)-induced hippocampal disorders: highlights on therapeutic potential of Naringenin and Quercetin. IBRO Rep. 6, 137–146. doi: 10.1016/j.ibror.2019.04.002
Al-Amin, M. M., Choudhury, M. F. R., Chowdhury, A. S., Chowdhury, T. R., Jain, P., Kazi, M., et al. (2018). Pretreatment with risperidone ameliorates systemic LPS-induced oxidative stress in the cortex and hippocampus. Front. Neurosci. 12:384. doi: 10.3389/fnins.2018.00384
André, S., Heinrich, S., Kayser, F., Menzler, K., Kesselring, J., Khader, P., et al. (2016). At-home tDCS of the left dorsolateral prefrontal cortex improves visual short-term memory in mild vascular dementia. J. Neurol. Sci. 369, 185–190. doi: 10.1016/j.jns.2016.07.065
Belarbi, K., Jopson, T., Tweedie, D., Arellano, C., Luo, W., Greig, N. H., et al. (2012). TNF-α protein synthesis inhibitor restores neuronal function and reverses cognitive deficits induced by chronic neuroinflammation. J. Neuroinflam. 9:23.
Belkhelfa, M., Beder, N., Mouhoub, D., Amri, M., Hayet, R., Tighilt, N., et al. (2018). The involvement of neuroinflammation and necroptosis in the hippocampus during vascular dementia. J. Neuroimmunol. 320, 48–57. doi: 10.1016/j.jneuroim.2018.04.004
Bin, L., Liu, J., Jinxia, Z., Wenjing, M., and Shiying, L. (2019). Effects of autophagy on synaptic-plasticity-related protein expression in the hippocampus CA1 of a rat model of vascular dementia. Neurosci. Lett. 707:134312. doi: 10.1016/j.neulet.2019.134312
Cechetti, F., Worm, P. V., Pereira, L. O., Siqueira, I. R., and Netto, C. A. (2010). The modified 2VO ischemia protocol causes cognitive impairment similar to that induced by the standard method, but with a better survival rate. Braz. J. Med. Biol. Res. 43, 1178–1183. doi: 10.1590/s0100-879x2010007500124
Charvet, L. E., Dobbs, B., Shaw, M. T., Bikson, M., Datta, A., and Krupp, L. B. (2018). Remotely supervised transcranial direct current stimulation for the treatment of fatigue in multiple sclerosis: results from a randomized, sham-controlled trial. Mult. Scler. 24, 1760–1769. doi: 10.1177/1352458517732842
Chen, A., Xiong, L., Tong, Y., and Mao, M. (2013). Neuroprotective effect of brain-derived neurotrophic factor mediated by autophagy through the PI3K/Akt/mTOR pathway. Mol. Med. Rep. 8, 1011–1016. doi: 10.3892/mmr.2013.1628
Chengbiao, L., Yun, W., Rui, H., Yong, W., Kun, L., and Xiaoli, L. (2015). Transcranial direct current stimulation ameliorates behavioral deficits and reduces oxidative stress in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-Induced mouse model of Parkinson’s disease. Neuromodulation 18, 442–447. doi: 10.1111/ner.12302
Chunjiea, N., Huijuan, N., Zhao, Y., Jianzhao, W., and Xiaojian, Z. (2016). Betaine reverses the memory impairments in a chronic cerebral hypoperfusion rat model. Neurosci. Lett. 615, 9–14. doi: 10.1016/j.neulet.2015.11.019
Datta, A., Bikson, M., and Fregni, F. (2010). Transcranial direct current stimulation in patients with skull defects and skull plates: high-resolution computational FEM study of factors altering cortical current flow. Neuroimage 52, 1268–1278. doi: 10.1016/j.neuroimage.2010.04.252
Du, S.-Q., Wang, X.-R., Xiao, L.-Y., Tu, J.-F., Zhu, W., He, T., et al. (2017). Molecular mechanisms of vascular dementia: what can be learned from animal models of chronic cerebral hypoperfusion? Mol. Neurobiol. 54, 3670–3682. doi: 10.1007/s12035-016-9915-1
Dugan, L. L., Ali, S. S., Shekhtman, G., Roberts, A. J., Lucero, J., Quick, K. L., et al. (2009). IL-6 mediated degeneration of forebrain GABAergic interneurons and cognitive impairment in aged mice through activation of neuronal NADPH oxidase. PLoS One 4:e5518. doi: 10.1371/journal.pone.0005518
Elder, G., and Taylor, J. (2014). Transcranial magnetic stimulation and transcranial direct current stimulation: treatments for cognitive and neuropsychiatric symptoms in the neurodegenerative dementias? Alzheimers Res. Ther. 6:74. doi: 10.1186/s13195-014-0074-1
Fan, Y., Du, L., Fu, Q., Zhou, Z., Zhang, J., Li, G., et al. (2018). Inhibiting the NLRP3 inflammasome with MCC950 ameliorates isoflurane-induced pyroptosis and cognitive impairment in aged mice. Front. Cell. Neurosci. 12:426. doi: 10.3389/fncel.2018.00426
Gomes, M., Akiba, H., Gomes, J., Trevizol, A., Lacerda, A. D., and Dias, Á (2019). Transcranial direct current stimulation (tDCS) in elderly with mild cognitive impairment: a pilot study. Dement. Neuropsychol. 13, 187–195. doi: 10.1590/1980-57642018dn13-020007
Guo, F., Lou, J., Han, X., Deng, Y., and Huang, X. (2017). Repetitive transcranial magnetic stimulation ameliorates cognitive impairment by enhancing neurogenesis and suppressing apoptosis in the hippocampus in rats with ischemic stroke. Front. Physiol. 8:559. doi: 10.3389/fphys.2017.00559
Jiang, W., Li, B., Chen, Y., and Gao, S. I. (2017). The toxic influence of dibromoacetic acid on the hippocampus and pre-frontal cortex of rat: involvement of neuroinflammation response and oxidative stress. Metab. Brain Dis. 32, 2009–2019. doi: 10.1007/s11011-017-0095-0
Kitazawa, M., Cheng, D., Tsukamoto, M., Koike, M., Wes, P., Vasilevko, V., et al. (2011). Blocking IL-1 signaling rescues cognition, attenuates tau pathology, and restores neuronal β-catenin pathway function in an Alzheimer’s disease model. J. Immunol. 187, 6539–6549. doi: 10.4049/jimmunol.1100620
Laste, G., Caumo, W., Adachi, L. N. S., Rozisky, J. R., Macedo, I. C. D., Filho, P. R. M., et al. (2012). After-effects of consecutive sessions of transcranial direct current stimulation (tDCS) in a rat model of chronic inflammation. Exp. Brain Res. 221, 75–83. doi: 10.1007/s00221-012-3149-x
Lee, S. B., Kim, H. T., Yang, H. O., and Jang, W. (2018). Anodal transcranial direct current stimulation prevents methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced neurotoxicity by modulating autophagy in an in vivo mouse model of Parkinson’s disease. Sci. Rep. 8:15165.
Leffa, D. T., Bellaver, B., Salvi, A. A., Oliveira, C. D., Caumo, W., Grevet, E. H., et al. (2018). Transcranial direct current stimulation improves long-term memory deficits in an animal model of attention-deficit/hyperactivity disorder and modulates oxidative and inflammatory parameters. Brain Stimul. 11, 743–751. doi: 10.1016/j.brs.2018.04.001
Lin, M. S., Chiu, M. J., Wu, Y. W., Huang, C. C., Chao, C. C., Chen, Y. H., et al. (2011). Neurocognitive improvement after carotid artery stenting in patients with chronic internal carotid artery occlusion and cerebral ischemia. Stroke 42, 2850–2854. doi: 10.1161/STROKEAHA.111.613133
Liu, B., Gao, J. M., Li, F., Gong, Q. H., and Shi, J. S. (2018). Gastrodin Attenuates Bilateral Common Carotid Artery Occlusion-Induced Cognitive Deficits Via Regulating Aβ-related proteins and reducing autophagy and apoptosis in rats. Front. Pharmacol. 9:405. doi: 10.3389/fphar.2018.00405
Lu, C., Wei, Y., Hu, R., Wang, Y., Li, K., and Li, X. (2015). Transcranial direct current stimulation ameliorates behavioral deficits and reduces oxidative stress in 1-Methyl-4-Phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of Parkinson’s disease. Neuromodulation 18, 442–446. doi: 10.1111/ner.12302
Manenti, R., Cotelli, M., Cobelli, C., Gobbi, E., Brambilla, M., Rusich, D., et al. (2018). Transcranial direct current stimulation combined with cognitive training for the treatment of Parkinson disease: a randomized, placebo-controlled study. Brain Stimul. 11, 1251–1262. doi: 10.1016/j.brs.2018.07.046
Meinzer, M., Lindenberg, R., Phan, M., Ulm, L., Volk, C., and Flöel, A. (2015). Transcranial direct current stimulation in mild cognitive impairment: behavioral effects and neural mechanisms. Alzheimers Dement. 11, 1032–1040. doi: 10.1016/j.jalz.2014.07.159
Mirzapour, S., Rafieirad, M., and Rouhi, L. (2015). Hydroalcoholic extract of ferulago angulata improves memory and pain in brain hypoperfusion ischemia in rats. Jundishapur. J. Nat. Pharm. Prod. 10:e17451.
Moloudizargari, M., Asghari, M., Ghobadi, E., Fallah, M., Rasouli, S., and Abdollahi, M. (2017). Autophagy, its mechanisms and regulation: implications in neurodegenerative diseases. Ageing Res. Rev. 40, 64–74. doi: 10.1016/j.arr.2017.09.005
Ohtaki, H., Mori, S., Nakamachi, T., Dohi, K., Yin, L., Endo, S., et al. (2003). Evaluation of neuronal cell death after a new global ischemia model in infant mice. Acta Neurochir. Suppl. 86, 97–100. doi: 10.1007/978-3-7091-0651-8_22
Oliveira, C. D., Freitas, J. S. D., Macedo, I. C., Scarabelot, V. L., Ströher, R., Santos, D. S., et al. (2019). Transcranial direct current stimulation (tDCS) modulates biometric and inflammatory parameters and anxiety-like behavior in obese rats. Neuropeptides 73, 1–10. doi: 10.1016/j.npep.2018.09.006
Pikhovych, A., Stolberg, N. P., Flitsch, L. J., LuiseWalter, H., Graf, R., Fink, G. R., et al. (2016). Transcranial direct current stimulation modulates neurogenesis and microglia activation in the mouse brain. Stem Cells Int. 2016:2715196. doi: 10.1155/2016/2715196
Podda, M. V., Cocco, S., Mastrodonato, A., Fusco, S., Leone, L., Barbati, S. A., et al. (2016). Anodal transcranial direct current stimulation boosts synaptic plasticity and memory in mice via epigenetic regulation of Bdnf expression. Sci. Rep. 6:22180. doi: 10.1038/srep22180
Rueger, M. A., Keuters, M. H., Walberer, M., Braun, R., Klein, R., Sparing, R., et al. (2012). Multi-session transcranial direct current stimulation (tDCS) elicits inflammatory and regenerative processes in the rat brain. PLoS One 7:e43776. doi: 10.1371/journal.pone.0043776
Scelzo, E., Giannicola, G., Rosa, M., Ciocca, M., Ardolino, G., Cogiamanian, F., et al. (2011). Increased short latency afferent inhibition after anodal transcranial direct current stimulation. Neurosci. Lett. 498, 167–170. doi: 10.1016/j.neulet.2011.05.007
Schoenfeld, R., Schiffelholz, T., Beyer, C., Leplow, B., and Foreman, N. (2017). Variants of the Morris water maze task to comparatively assess human and rodent place navigation. Neurobiol. Learn. Mem. 139, 117–127. doi: 10.1016/j.nlm.2016.12.022
Shibata, M., Ohtani, R., Ihara, M., and Tomimoto, H. (2004). White matter lesions and glial activation in a novel mouse model of chronic cerebral hypoperfusion. Stroke 35, 2598–2603. doi: 10.1161/01.str.0000143725.19053.60
Venkat, P., Chopp, M., Zacharek, A., Cui, C., Landschoot-Ward, J., Qian, Y., et al. (2018). Sildenafil treatment of vascular dementia in aged rats. Neurochem. Int. 127, 103–112. doi: 10.1016/j.neuint.2018.1012.1015
Wang, D. P., Yin, H., Kang, K., Lin, Q., Su, S. H., and Hai, J. (2018). The potential protective effects of cannabinoid receptor agonist WIN55,212-2 on cognitive dysfunction is associated with the suppression of autophagy and inflammation in an experimental model of vascular dementia. Psychiatry Res. 267, 281–288. doi: 10.1016/j.psychres.2018.06.012
Yang, W.-J., Wen, H.-Z., Zhou, L.-X., Luo, Y.-P., Hou, W.-S., Wang, X., et al. (2019). After-effects of repetitive anodal transcranial direct current stimulation on learning and memory in a rat model of Alzheimer’s disease. Neurobiol. Learn. Mem. 161, 37–45. doi: 10.1016/j.nlm.2019.02.002
Yu, T.-H., Wu, Y.-J., Chien, M.-E., and Hsu, K.-S. (2019). Transcranial direct current stimulation induces hippocampal metaplasticity mediated by brain-derived neurotrophic factor. Neuropharmacology 144, 358–367. doi: 10.1016/j.neuropharm.2018.11.012
Keywords: vascular dementia, tDCS, oxidative stress, inflammation, autophagy
Citation: Guo T, Fang J, Tong ZY, He S and Luo Y (2020) Transcranial Direct Current Stimulation Ameliorates Cognitive Impairment via Modulating Oxidative Stress, Inflammation, and Autophagy in a Rat Model of Vascular Dementia. Front. Neurosci. 14:28. doi: 10.3389/fnins.2020.00028
Received: 13 May 2019; Accepted: 10 January 2020;
Published: 29 January 2020.
Edited by:Shapour Jaberzadeh, Monash University, Australia
Reviewed by:Giuseppe D’Avenio, Istituto Superiore di Sanità (ISS), Italy
Jie Liu, Sichuan University, China
Zuo Wang, University of South China, China
Copyright © 2020 Guo, Fang, Tong, He and Luo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Jia Fang, firstname.lastname@example.org

domain: medical


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Who could've guessed that that the most reliable band to come out of Seattle's grunge period would be… Mudhoney? Not even front man Mark Arm saw that coming. When Seattle became an alt-rock boomtown in the early '90s, Mudhoney stayed comfortably under the radar. There were no platinum records, no revolving door of band members, no reunion tours. Just a lot of concerts and albums filled with gleefully aggressive guitar rock and punk energy. Twenty-five years later, original bandmates Arm, Steve Turner, and Dan Peters soldier on—with Guy Maddison, who joined as bassist in 2001—and are set to release their ninth studio album of disheveled rock fury, Vanishing Point, on April 2. This weekend, the band takes the stage with '60s garage rock pioneers the Sonics for a sold-out show at Showbox at the Market.
For our latest Fiendish Conversation, we talked to Arm about Mudhoney’s new record, the Sonics' sound, and MySpace (sort of).
This year marks the 25th anniversary of Sub Pop and you guys are the stalwarts…
It’s also the 25th anniversary of our band. We actually mark our birthday before Sub Pop does.
When do you officially mark your birthday?
The first practice with all four original members was on New Year's Day, 1988. None of us have a real good recollection of it. I’m sure we were pretty fucked. (Laughs) But that was the first day. Dan [Peters] and Steve [Turner] and I had been kind of playing a little bit in December and maybe even as early as November of ’87, but that was the first time that we played with Matt [Lukin]. He was coming into town for some New Year’s Eve shenanigans and we got together the next day.
So were you kind of just screwing around at that point? I mean, I’m sure you didn’t think you’d still be in the same band 25 years later.
(Laughs) No, no, no, no, no, no, no, no, no. It wasn’t totally messing around; we had some ideas for songs by the time that Matt had shown up, so it wasn’t like the four of us getting together and jamming. But no, no. Our main goal was to put out a single, and that was all that we could see into the future at that point. If you would have told me then that we would still be around, I would’ve just thought that you were nuts. But this is something that has sort of crept up slowly over time—the new normal.
This might be a clichéd question, but what’s been the key to making it last so long?
A couple things in our case: One thing is we’ve just split up publishing equally and credit songs to the band. No one’s fighting for a piece of the action, fighting over pennies or anything like that. Another thing is we give a shit about what we do but we just don’t give a shit. If that makes any sense. We’re not aiming for the brass ring; we never were. It just sort of seemed like a ridiculous idea to us at the time. The fact that some of our friends actually managed to grab it is really, really weird. Like, “Wow. This is something new and different.” Because the scene that we came up in—the '80s underground music scene—the idea of selling 100,000 records… that was insane.
What does the new album, Vanishing Point, have in store?
There aren’t any extreme radical departures. We’re still playing our own brand of dance music. There are things that I think are kind of new to this record, but it still sort of feels in the realm of Mudhoney. There are a couple songs that are stylistically somewhat different than anything we’ve released before. At least it feels that way to me. I dunno, someone else might just go, “Oh, that sounds like the same old shit from Mudhoney.”
For those who might be unfamiliar, can you talk about the importance of the Sonics?
Trying to convince somebody else of the importance of something else… I don’t know if it always translates. I don’t know if you can make someone get it.
I can tell you when I first heard them was around 1980. I was getting into hardcore punk and I read this review in Trouser Press magazine about this band that sounded intriguing, but they were from the '60s. And the records had just gotten reissued on Etiquette, kind of a poor reissuing job because the covers were so contrasty and blown out, but I put it on and I was hooked. I immediately saw the connection from what they were doing and what I was into. And this was early- to mid-'60s. It kind of made me feel that there was a historical thread that ran through to the music that I was into even though hardcore sort of seemed like something that was brand new at the time. But it was just derived from punk rock, which had its roots in the more stripped down and aggressive aspects of rock and roll. The guitar sound on the Sonics’ records is really gnarly, the drumming is ferocious and relentless, and Gerry Roslie has one of the best screams in rock. His singing was definitely a huge influence on mine.
How do you feel Seattle influenced your sound?
Early on, the biggest influence Seattle had in terms of getting into music and playing the music that I liked was that it was so off the beaten path. No one gave a shit about Seattle. Bands could do stuff for years in obscurity and actually find their own footing. But that’s definitely not the way Seattle is now. I don’t think there’s anyplace in the world like that now. With MySpace all over the place! Right?!? MySpace?
If you weren’t a musician what other line of work might you have pursued?
When I was a kid, my plan was to be a writer. Kind of fucked that up.
The Sonics and Mudhoney
Feb 2, Showbox at the Market, sold out
Mudhoney (Vanishing Point Release Show)
Mar 30, Neumos, $15
Easy Street Records In-Store Concert with Mudhoney
Apr 1 at 8, Easy Street West Seattle, free
Note: The song contains profanity.

domain: artistic


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Combining our insights from the research and prototyping stages, we present: AdaptiveWatch - a future-focused TV-viewing experience we envision would be integrated as a toggle-able feature across all TV streaming platforms.
AdaptiveWatch utilizes user-purchased sensors, such as smartwatches, cameras, and smart-speakers to extract user’s emotion, attention, and personal viewing habits. Using that information, it will leverage Deep Learning models to synthetically adapt the TV content in real time based on the user’s context, thereby seamlessly and dynamically adjusting the TV content to meet the user’s emotional, cognitive, and personal viewing needs. AdaptiveWatch uses real-time stylistic changes to the plot-line, weather, music, and color grading to adapt the content according to the user's emotional and personal viewing preferences to enhance the TV viewing experience.
For our high-fidelity prototype of the AdaptiveWatch experience, we put a spin on the classic European fairy tale Little Red Riding Hood. The narrative that we all know - a young girl who goes to visit her grandmother in the wood, a wolf follows her and eats Little Red and her grandmother, and a hunter rescues them. This is the base narrative that we used as the foundation of our prototype’s plotline. Along with the base narrative, we created three other narratives with themes of sadness, horror, and comedy (respectively) in order to simulate an on-the-fly plot adaptation based on users’ emotional inputs.
Aside from the plot-line adaption, we also incorporated stylistic changes - including weather, background music, and color grading - to put the variables to the test and determine which change(s) users prefer to experience during their viewing session.
While the goal for context-aware content is to have the narrative adapting automatically based on the user's emotional responses, we learned from early prototyping that users have mixed emotions about the automated viewing experiences. It's an experience they want to choose to have, not expect.
Integration into existing streaming platforms and the ability to toggle the feature on and off is imperative. AdaptiveWatch is a feature, a supplement, a way to enhance your viewing, but it's not the rule.
Our team used a variety of brainstorming activities, research methods, synthesis and design methodologies to narrow down our scope and get to the root of the problem we aimed to solve for, including:
pretotyping, contextual inquiries, diary studies, affinity diagramming, experience mapping, and needfinding exercises.
How do users consume digital content now?
How do users create digital content now?
What do people value about the TV viewing experience?
Through needfinding research, we analyzed people’s ideas in a future-thinking imagination game to identify their potential hopes, fears, and expectations for synthetic content and TV-related technology. We found that people want to consume content for:
Read more about our research here.
Although this project reached its conclusion with a prototype that is geared towards the solo viewing experience, there is so much more to the world of content viewing that could be explored further. What about the group viewing experience? Or viewing content on-the-go in a mobile setting? There are as many ways to view content as there are viewers, which means that a system of adaptive content has vast potential beyond just the solo viewing context.
I really enjoyed examining the content viewing experience and envisioning a new kind of future for television in the digital age with the rest of my team. This project helped me develop my leadership and project management skills, while also giving me a taste for what it's like to work within an ambiguous future-thinking space. I'm very proud to have had this opportunity to work with my team and with our client.


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Sohrabuddin encounter case trial set to start in Mumbai court
Mumbai: Seven years after the killing of Sohrabuddin Sheikh in an allegedly fake encounter, a magistrate`s court here on Friday transferred the case to the special CBI court, paving the way for the start of the trial of nineteen accused, including former Gujarat minister Amit Shah.
"After completion of formalities the magistrate has committed the case to sessions court," said special public prosecutor Ejaz Khan.
The Supreme Court last year transferred the trial to Maharashtra from Gujarat, as CBI said the witnesses were being intimidated and the trial could not be held in a free and fair manner in that state.
Shah was the Minister of State for Home in Narendra Modi government before he stepped down as his name surfaced in the Sohrabuddin case. Shah recently won an Assembly election.
Sohrabuddin and his wife Kauser Bi were allegedly abducted by Gujarat`s Anti-Terrorism Squad from Hyderabad and killed in a fake encounter near Gandhinagar in November 2005. Further, Tulsiram Prajapati, a key witness to encounter, was allegedly killed by the police in Gujarat`s Banaskantha district in December 2006.
According to CBI, Shah was the "kingpin" of the conspiracy to kill Sohrabuddin. Most of the other accused in the case are policemen, some of them IPS officers.
Shah was arrested in July 2010 but released on bail after three months. A close confidante of Narendra Modi, Shah is currently out on bail.
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domain: politics


Obama’s trip to a Toledo plant that assembles Jeep Wranglers follows a week of White House publicity about the automotive revival, including a May 28 radio address by Vice President Joe Biden and a June 1 White House report saying $80 billion of federal aid for Chrysler andGeneral Motors Co. (GM) saved at least 1 million jobs at automakers and their suppliers.
The president also will have some good news to deliver for taxpayers:Fiat SpA (F) and the U.S. Treasury Department announced last night that they have reached an agreement for the Italian auto company to buy the government’s remaining 6 percent stake in Chrysler for $500 million.
Obama’s task is to persuade voters that, while recovery has been slow in coming, the recession would have been far worse without government action to keep GM and Chrysler in business, said Steven Rattner, the former head of the president’s automotive task force.
“It’s very hard to convince people of what would have happened if you didn’t do something,” Rattner said. “People hate bailouts. They are angry about a lot of things and it’s a knee-jerk reaction, without a substantive foundation.”
“The administration’s auto bailout is nothing to celebrate,” said Brendan Buck, a spokesman for House Speaker John Boehner, an Ohio Republican. “There were better options that could have saved jobs for these workers. The model the White House should be touting is Ford, which, instead of relying on a taxpayer-funded bailout, saw trouble coming and made the tough decisions necessary to preserve jobs and weather the storm.”


Traverse Dynastretch Shorts are sports shorts for men for training during the Spring – Summer season. The version has waterproof features to ensure that moisture does not come into contact with your skin.
This athletic clothing is made of elastic fabric with 4-way stretch to allow a proper range of motion.
On the front, the Dynafit shorts have two zippered pockets to keep your belongings safe. The version is available in a variety of sizes and colors.
With the comfortable Traverse Dynastretch Shorts for women, you are always perfectly outfitted for summer mountaineering. On fast tours, you need pants that don’t bind or make you sweat any more. The Dynastretch Traverse Shorts offer the best assistance to be able to dig extra deep on hot days. They offer an impressive modern design, high breathability, and maximum freedom of movement thanks to 4-way stretch. The fabric wears very comfortably next-to-skin and is abrasion-resistant despite its lightness. That means you don’t have to shy away from any contact with rocks. A stretch waist with drawstring cord adjustment ensures a stay-put fit and comfort at any time on the mountain Perforations on the back, where your backpack sits, improves air circulation and provides that extra bit of cooling power on demanding climbs. These sporty shorts are rounded out with two zip pockets to stash small essentials. Traverse Dynastretch Shorts – modern, airy, lightweight.
– 4 Way Stretch
– Abrasion resistant
– Active Airflow Ventilation
– Adjustable Waist
– Elastic waist
– Water Repellent
– Wind Resistant
– Zipped front pockets
– Pocket Lining: WARPKNIT MESH POLYGIENE 90 g/sqm (100% Polyester)
– Main material: DYNASTRETCH DOTS PFC FREE DWR 150 (65% Polyamide 21% Elastane 14% Polyester)
– Back Insert: NYLON ACTIVE DOBBY DWR 106 (85% Polyamide 15% Elastane)
– Weight: 131 g
– Layers: Baselayer
– Do not dry clean
– Do not chlorine bleach
– Do no tumble dry
– Machine wash cool synthetics
– Cold ironing
Do you want to know which other related running and triathlon products Dynafit has? At runnerinn, you can buy all the necessary Pants items so that you can benefit from your activities without complications. Dynafit Traverse Dynastretch Shorts is at best price and in stock! Make us of our secure online payment system and benefit from our high qualified salesteam.


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Check the way to make a special photo charms, and add it into your Pandora bracelets. Painted Cactus Rocks Tutorial - all you need are stones and paints to create these stunning cacti.
pallet Pallet herbs planters in vertical garden urban planter 2 flowers 2 with pallet planter pallet herbs
Use beige board for this!
This idea rocks!
From pallet to lined & raised vegetable bed.
Acras de crevettes épicées, ail, coriandre, piment.


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Daytona Beach Shores is a very small coastal city (i.e. on the ocean, a bay, or inlet) located in the state of Florida. With a population of 4,614 people and two constituent neighborhoods, Daytona Beach Shores is the 290th largest community in Florida.
Housing costs in Daytona Beach Shores are among some of the highest in the nation, although real estate prices here don't compare to real estate prices in the most expensive communities in Florida.
Unlike some cities, Daytona Beach Shores isn’t mainly white- or blue-collar. Instead, the most prevalent occupations for people in Daytona Beach Shores are a mix of both white- and blue-collar jobs. Overall, Daytona Beach Shores is a city of professionals, sales and office workers, and managers. There are especially a lot of people living in Daytona Beach Shores who work in teaching (11.24%), office and administrative support (9.64%), and management occupations (9.29%).
Of important note, Daytona Beach Shores is also a city of artists. Daytona Beach Shores has more artists, designers and people working in media than 90% of the communities in America. This concentration of artists helps shape Daytona Beach Shores’s character.
One interesting thing about the economy is that relatively large numbers of people worked from their home: 13.26% of the workforce. While this number may seem small overall, as a fraction of the total workforce this is high compared to the rest of the county. These workers are often telecommuters who work in knowledge-based, white-collar professions. For example, Silicon Valley has large numbers of people who telecommute. Other at-home workers may be self-employed people who operate small businesses out of their homes.
Another notable thing is that Daytona Beach Shores is a major vacation destination. Much of the city’s population is seasonal: many people own second homes and only live there part-time, during the vacation season. The effect on the local economy is that many of the businesses are dependent on tourist dollars, and may operate only during the high season. As the vacation season ends, Daytona Beach Shores’s population drops significantly, such that year-round residents will notice that the city is a much quieter place to live.
One of the nice things about Daytona Beach Shores is that it is nautical, which means that parts of it are somewhat historic and touch the ocean or tidal bodies of water, such as inlets and bays. Because of this, visitors and locals will often go to these areas to take in the scenery or to enjoy waterfront activities.
Daytona Beach Shores is one of the most well-educated cities in the nation. 43.00% of adults in Daytona Beach Shores have at least a bachelor's degree. Compare that to the average community in America, which has just 21.84% with a bachelor's degree or higher.
The per capita income in Daytona Beach Shores in 2018 was $48,303, which is wealthy relative to Florida and the nation. This equates to an annual income of $193,212 for a family of four. However, Daytona Beach Shores contains both very wealthy and poor people as well.
The people who call Daytona Beach Shores home describe themselves as belonging to a variety of racial and ethnic groups. The greatest number of Daytona Beach Shores residents report their race to be White, followed by Black or African-American. Important ancestries of people in Daytona Beach Shores include Irish, English, German, Italian, and Polish.
The most common language spoken in Daytona Beach Shores is English. Other important languages spoken here include Spanish and Other Asian languages.

domain: economics


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Despite the losses, it looks like IU is in a stronger position this season than they were last season.
Sprint free/fly specialist Chris Holmes from Carmel High School and Carmel Swim Club will head to Indiana University in the fall.
In this video International Swimming League (ISL) partner Wasserman Media Group explains their research into launching an international pro swimming league.
Launching an international pro swimming league is an enormous undertaking. While the concept has been around since the 1980s, since…


A collection of news and information related to Florida Elections published by this site and its partners.
Displaying items 1-12 of 76 » View baltimoresun.com items only1 2 3 4 5 6 7 Next >
In 2000 when the votes for the Gore-Bush presidential race were still being counted in Florida long after Election Day, amid charges of voter fraud and suppression, I wrote a column about the importance of every single ballot being counted. I'm sure...
Tags: Polls, Barack Obama, Elections, Voting, Voting Machines
Sun ReporterVice President Al Gore filed a brief with the Florida Supreme Court yesterday, asking it to overturn a judicial ruling that threw out his election challenge and to order an immediate manual recount of contested ballots that he believes will bring him...
Sun ReporterWith the presidency at stake, the Supreme Court will assemble today to seek a constitutional formula for ending the conflict over the last unsettled electoral votes - Florida's decisive 25. The nine justices, returning to their courtroom for the second...
Sun ReporterDisplaying statistical charts and voting machines brimming with chads, two expert witnesses for Vice President Al Gore testified yesterday about the likelihood that a machine count of Election Day ballots overlooked votes for president. The testimony...
Sun ReporterThe U.S. Supreme Court, its deep division on full public display, stopped the vote counting in Florida yesterday and set the stage for a ruling that could make George W. Bush the next president. By a vote of 5-4, the court blocked a 22-hour-old ruling by...
Sun ReportersJust when it seemed Vice President Al Gore was gasping his last in a marathon struggle for the White House, the Florida Supreme Court gave him a shot of oxygen, ordering the manual recount of more than 40,000 contested Florida ballots that he hopes will...
Sun ReporterMindful of the extraordinary dispute before it, the Florida Supreme Court pressed lawyers for Al Gore and George W. Bush yesterday to answer whether they should intervene in the contested presidential election and resolve the case by Tuesday when the...
Sun ReporterGeorge W. Bush's campaign said yesterday that a recount confirmed that he had won Florida's election and called on Al Gore to reconsider his decision to support court fights and further recounts in the state. But the Gore campaign rejected the...
Sun ReporterTaking a page from a Perry Mason script, lawyers for Vice President Al Gore turned an expert witness for Texas Gov. George W. Bush into an ally in their fight for a hand count of contested Florida ballots that could overturn the presidential election....
Tallahassee Bureau ChiefTALLAHASSEE -- On the final day of session, the Florida Legislature answered the national elections scorn the state absorbed over its long lines and tortoise pace in declaring Barack Obama its 2012 presidential winner. Lawmakers on Friday sent Gov. Rick...
Tallahassee Bureau ChiefTALLAHASSEE -- Backers of a sweeping sports stadium package were working furiously Friday to convince the Florida House to pass tax-breaks for the Miami Dolphins, Orlando soccer boosters, Daytona International Speedway and others. Central Florida Sens....
When Bob Graham speaks, people should listen. If he tells you something, you can take it to the bank. Graham's integrity resulted in his never losing an election during 38 years in politics, including eight as Florida's governor and 18 as a U.S....
Nov 20, 2012 |Story| Patuxent Homestead
Dec 7, 2000 |Story| Baltimore Sun
Dec 11, 2000 |Story| Baltimore Sun
Dec 3, 2000 |Story| Baltimore Sun
Dec 10, 2000 |Story| Baltimore Sun
Dec 9, 2000 |Story| Baltimore Sun
Dec 8, 2000 |Story| Baltimore Sun
Nov 11, 2000 |Story| Baltimore Sun
Dec 4, 2000 |Story| Baltimore Sun
May 3, 2013 | Orlando Sentinel
May 3, 2013 | Orlando Sentinel
Feb 24, 2013 |Column| South Florida Sun-Sentinel
Original site for Florida Elections topic gallery.


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SIOUX FALLS, S.D. (KELO) — There were 415 new total COVID-19 cases reported on Monday, bringing the state’s total case count to 162,790, up from Friday (162,375).
The number of active cases reported on Monday is at 6,865, up from Friday (6,820).
There were four new deaths reported, bringing the total to 2,309. The new deaths include two men and two women in the following age ranges: 60-69 (1); 80+ (3). The new deaths were in Codington, Hughes, Minnehaha and Pennington counties.
Current hospitalizations are at 230, down from Friday (234). Total hospitalizations are at 8,271, up from Friday (8,252).
Total recovered cases are now at 153,616, up from Friday (153,250).
The DOH currently reports total tests each day. There have been 1,681,312 total tests reported as of Monday, up 3,839 from 1,677,473 total tests reported on Friday.
The latest seven-day PCR test positivity rate for the state is 15.6% for Nov. 12 – 18.
Of South Dakota’s 66 counties, 65 are listed as having “high” or “substantial” community spread. “High” community spread is 100 cases or greater per 100,000 or a 10% or greater PCR test positivity rate.
There have been 893 Delta variant cases (B.1.617.2, AY.1-AY.25) detected in South Dakota through sentinel monitoring.
There have been 172 cases of the B.1.1.7 (Alpha variant), 3 cases of P.1. (Gamma variant) and 2 cases of the B.1.351 (Beta variant).
The DOH announced changes to how it reports vaccinations on the COVID-19 dashboard as of Monday, October 14. It now includes a breakout of how many people have received booster doses. Due to data clean-up efforts, the percentages of people who’ve received one dose or completed the series have changed. Find the DOH explanation in this story.
As of Monday, 70.54% the population 12-years-old and above has received at least one dose while 59.07% have completed the vaccination series. 14.79% of those eligible have completed their booster dose.
There have been 546,299 doses of the Pfizer vaccine administered, 391,103 of the Moderna vaccine and 32,912 doses of the Janssen vaccine.
There have been 165,300 persons who have completed two doses of Moderna. There have been 226,927 persons who have received two doses of Pfizer.
As for booster doses, 60,994 people have received a 3rd Pfizer shot, 42,106 have received a 3rd Moderna dose and 1,087 have received a Janssen booster.
The state is also reporting data for the number of children under 12 years of age who have received a vaccine. It shows 7,156 children have gotten the vaccine, which is 4.88% of that population.


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World War II remains in the public consciousness as a certain businessman, currently running for President of the United States, is evoking comparisons to a certain Austrian in power in Germany in 1941. Kim Brooks’ first novel reminds us that those who cannot remember the past are condemned to repeat it. The Houseguest begins in the summer of 1941, when the war in Europe was happening to other people, when a boatload of Jewish refugees, denied entry into Cuba, was also turned away at the port of Miami, left to return to Europe or die at sea.
Brooks has assembled a compelling cast of characters. In Utica, New York, a junkman and his wife take in a refugee, an actress who refuses to conform to the role of grateful houseguest. The couple’s rabbi, Max Hoffmann, finds himself acting as eyes and ears for Shmuel Spiro, the head of the Committee for a Jewish Army of Stateless and Palestinian Jews, at conferences where Spiro isn’t welcome. And refugee Ana Beidler bewitches Abe Auer, her host, while bedeviling men from her past.
A comparison to the present notwithstanding, this is a powerful book. There are very few Gentile characters, so this allows for more nuanced portraits of its Jewish characters. They exist not as counterpoints to Gentiles but to each other. As Spiro puts it, there are Jews in America and there are American Jews, and there is a difference. And tragedy is writ large in the past of several characters; they have been tested long before Hitler came to power. By the end of the book, the Japanese have bombed Pearl Harbor, so war has come to the United States.

domain: history


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Mobiles aren’t just for the nursery. We’ve found some great new designers that are making chic pieces for the home—we're calling it mobile art! Let’s take a look at our top picks: Atomic Mobiles – we love the mid-century feeling of these designs and the huge variety of color options. SCROLL THROUGH TO SEE ALL APPS> -Nina Freudenberger from thecultivatedhome.com Nina Freudenberger attended Rhode Island School of... CONTINUE READING 11
More articles about mobiles
COPYRIGHT © SHECKY'S MEDIA, INC. 2000-2013. WHERE A GIRL CAN DISCOVER, EXPERIENCE AND GET REWARDED WITH HER GIRLFRIENDS!


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Visit our FaceBook page to see what's going on right now!
The purpose of the North Attleborough Cultural Council is to promote and maintain the vitality of local cultural resources and to ensure these resources are shared within the North Attleborough community. The Council is committed to funding a diverse cross-section of activities. We support a broad variety of art forms, the ongoing work of individual artists, environmental education projects, collaborati ve proposals that bring together artists and other types of organizations, local cultural groups and projects that serve specific populations -youth, elders, low-income, and disabled.
In order to apply for an LCC grant, applicants must meet all eligibility and criteria requirements of the LCC Program.
For more detailed information, refer to the full LCC Program Guidelines (PDF).
An online application will be available September 1.
©2001-2017. Massachusetts Cultural Council. All rights reserved.
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The Ultimate Recipe Inspiration App
Try PERDUE® HARVESTLAND® Chicken, raised with No Antibiotics Ever. Click here to learn more.
BTotal Time1 hr 45 min
dried oregano, cooked turkey, salt and ground black pepper, butter and 9 MORE
pastry, double crust pie, condensed cream of chicken soup, salt and 9 MORE
water, knorr roasted turkey gravy mix, frozen mixed thawed vegetables, and 5 MORE
carrots, shredded cheddar cheese, salt, pastry, condensed cream of chicken soup and 4 MORE
I am a __age__ year old __gender__ who cooks for __family__

domain: food


Control-oriented Thermal Building Modelling
preprintposted on 2020-03-04, 10:03 authored by Sebastian TroitzschSebastian Troitzsch, Thomas Hamacher
Demand side flexibility (DSF) is becoming an important tool for the power system operation. With air-conditioned buildings being key candidates for DSF, this paper presents a control-oriented thermal building modelling approach which is kept in the linear domain and therefore suited for the employment in convex optimization problems. Along with the building model, two DSF indicators are proposed and applied to assess the DSF potential for an office building in Singapore.
Email Address of Submitting firstname.lastname@example.org
ORCID of Submitting Author0000-0002-1437-2185
Submitting Author's InstitutionTUMCREATE
Submitting Author's Country


The answer is A.B.C., which stands for Alpha, Bravo, Charlie, and Delta. Alpha: The first letter of the alphabet (A) represents the first letter in our name (John). So we just need to add one more letter to spell our name: J-O-H-N. Bravo: The second letter of the alphabet (B) represents the second letter in our name (J).
So we just need to add another two letters to spell our name: J-O-H-N. And so on until we reach Z, which stands for zero — because that’s how many letters this system uses! But wait a minute … what if you want your last initial? Well, then, you have to use an X as your final character! For example, John Smith would be spelled out as “JX” or “JSX,” — but don’t forget about adding those extra zeros at the end!
At AlienMinute, our writers and team from all over the world will cover hilarious and comical topics. We will cover social media gossips to sharing memes in hopes of connecting people from all over the world.


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Sean Anders Filmography updatesTabs
Total Updates: 16
- added Sean Anders as producer to movie credits of Countdown
- Sat., Sep. 23, 2017 from Variety
- added Sean Anders as producer, screenwriter and director to credits of Instant Family
- Fri., Apr. 22, 2016 from Deadline
- added Sean Anders as director and screenwriter to credits of Daddy's Home 2
- Fri., Jul. 24, 2015 from The Hollywood Reporter
- changed the status of Sean Anders to Former of Baywatch
- Fri., Nov. 7, 2014 from Paramount Pictures
- added Sean Anders as director to credits of Daddy’s Home
- Fri., Oct. 3, 2014 from The Hollywood Reporter
- added Sean Anders as director to credits of Baywatch
- Thu., Sep. 5, 2013 from Warner Bros. Pictures
- added Sean Anders as director to the film's credits of Horrible Bosses 2
- Sat., Nov. 3, 2012 from Variety
- added Sean Anders as Screenwriter (rewrite) to the credits of Mean Moms
- Sun., Jun. 3, 2012 from vulture.com
- changed the status of Sean Anders to Current of Three Mississippi
- Wed., Apr. 11, 2012 from The Hollywood Reporter
- added Sean Anders (In Talks) to the credits of Three Mississippi
- Thu., Oct. 27, 2011 from Deadline
- added Sean Anders to the credits of Dumb and Dumber To
- Tue., May. 3, 2011 from The Hollywood Reporter
- changed the status of Sean Anders to Former of We're the Millers
- Fri., Apr. 1, 2011 from The Wrap
- changed the status of Sean Anders to Current of That's My Boy
- Fri., Mar. 4, 2011 from Variety
- added Sean Anders (In Talks) to the credits of That's My Boy
- Wed., Sep. 8, 2010 from Los Angeles Times
- added Sean Anders (Rumored) to the credits of We're the Millers
- added Sean Anders to the credits of Mr. Popper's Penguins
Mark Wahlberg is set to star in the Paramount comedy Instant Family, sources confirm to Variety.
Paramount Pictures and Gary Sanchez Productions have set a sequel to Daddy’s Home.
Seth Gordon is negotiations to direct Baywatch, Paramount’s big-screen take on the 1990s television series about California lifeguards.
Paramount is moving forward with its big-screen adaptation of Baywatch.
Sean Anders and John Morris are ready to take on terrible mothers as the duo looks to pen the New Line movie Mean Moms.
We hear that Adam Sandler is in talks to star opposite Will Ferrell in Three Mississippi, a comedy from 30 Rock executive producer Robert Carlock and Friends executive producer Scott Silveri.
The team of Sean Anders and John Morris is in negotiations to rewrite the Warner Bros. project known as Turkey Bowl. Anders will direct.
Peter and Bobby Farrelly are moving forward with another project they’ve long wanted to do: a second installment of the New Line 1994 hit Dumb and Dumber.
Rawson Thurber is in negotiations to step into the director’s chair for the project, which centers on a drug dealer who, on his 30th birthday, decides that he wants out of the business.
Columbia Pictures is looking at James Caan to join the cast of "I Hate You Dad," a comedy also starring Andy Samberg and Adam Sandler, TheWrap has learned.
Sean Anders and John Morris, two of the hottest comedy writers in Hollywood, are in talks to direct the Adam Sandler-Andy Samberg comedy "I Hate You Dad" for Columbia Pictures.
"We're the Millers," a long-developed film that's now getting some new momentum. In the film, which is being developed by New Line, slackers aren't sitting around taking puffs and dreaming big. In fact the main character in this one, a veteran pot dealer, is actually pretty ambitious -- so ambitious that he decides to ...