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I really loved this movie Whale Rider. The story was about a tribe of people in New Zealand who were clinging on to their ancient culture and needed to find a leader. The natives of this land had such a rich cultural sphere and were very invested in learning about the ancient customs and using the stick fighting and the language, still in the present day. It seemed most of the people here treasured their ancient heritage and found solace in keeping their traditions very much alive. The story centered around a young girl named Pai and her relationship with her grandfather who has a difficult time accepting her because she was born a twin and her male twin brother died at birth. The story goes on to contemplate the strained relationship between Pai and her grandfather and that really all she wants from him is respect and love. In the end, all the things Pai has been fighting to prove to her father come true after some whales are beached (this culture believes that whales are gods of their ancestors.) and Pai is the only one to be able to get the largest whale free by just riding it. She almost drowns doing this and finally her father realizes exactly how important and special this little girl is. This story was full of heart and redemption and I think that is what made it so special.
Whale Rider17 11 2010


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Casey Cadwallader reinterprets the sensuality of corsetry with a futuristic twist at Mugler
Casey Cadwallader continues to define his contemporary vision of the Mugler aesthetic. Creative director of the L'Oréal-owned brand since the end of 2017, the American designer has once again opted for a spectacular demonstration of evocative elegance in order to show his collection for Fall/Winter 2021-22. Presented in digital form, his eight-minute fashion film, directed by Torso Solutions, is a kind of cinematic satire, in which models seem to pose for a photo shoot, giving repeated fourth-wall-breaking looks-to-camera, before stepping into a dynamic runway strut, all in front of a pure white background.
Supermodels Amber Valleta and Bella Hadid, as well as Madonna's headline-generating daughter, Lourdes Leon, were among those tasked with incarnating Mugler's celebration of femininity this season. This vampiric-looking cast strides in different directions in front of the lens, which constantly swivels, changes perspective and zooms in and out, contributing to the pervadingly dystopic and futuristic atmosphere, in which a series of figures dressed in white full-coverage onesies that blend into the background accompany the models at every turn, caressing them or holding them up.
The aesthetic is recognizable and easily identifiable. Pieces this characteristically sensual could only be from Mugler, but Cadwallader has also managed to create a collection that exudes freshness and modernity. His designs flitter back and forth between second-skin effects and experimentation with fabrics that are contorted into new, sculpted shapes and splashed with color and sparkle. The predominantly dark palette of oil-slick black, midnight blue, dusty pink and reflective silver incorporates coquettish flashes of striking blue-greens, neon yellow, orange and indigo, featured in overlapping tops, shirts and ripped dresses, as well as in bodysuits and skirts with side openings.
This attention-grabbing mix adapts Mugler's curvaceous aesthetic for a new generation, bringing in a few, somewhat more relaxed, touches of athleisure, while still staying true to the DNA of the brand, which is famous for its corsets. Here, these pieces abandon their rigidity in search of a new sensuality more appropriate for Instagram, the stage or the dancefloor. Reinterpreted via ultrasonic cutting, they are much lighter and more adapted to movement.
Other highlights include the fitted trousers creating the impression that the model is practically nude, the interplay of denim with black transparencies, the tight, corsetry-inspired jackets, and the keyhole shaped details on bodices, a clear allusion to the Mugler archives. And that's without mentioning the final stretch of the video, where serpentine golden chains cover the collection's last pieces, as well as the Jimmy Choo boots and sandals chosen to elevate the designs. For those wishing to continue their journey into the sensual world of Mugler, Paris' Musée des Arts Décoratifs is hosting the retrospective, "Thierry Mugler, Couturissime," until January 16, 2022.
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domain: artistic


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Breakfast huge amy of a large watermelon. Ate a whole 1/4 of a big heavy watermelon. Then drank
4 glasses water and then afternoon drank smoothie of hot pepper 1/2, cucumber and cilantro. Drank
this green spicy smoothie before went for late lunch with girlfriend to Ruby Tuesday for salad bar.
Ate only raw vegetables off the salad bar and ate without any dressing nor lemon just straight.
Must say I loved the peas and the lima beans. Suspect they were frozen and dethawed and not fresh like salad, carrots, cucumber, tomatoes but hey this is the healthiest meal I have ever eaten out. Imagine no salad dressing for me that is a big leap forward. Had the conviction of the tube videos to steer clear of the sodium filled sides and dressings. As msg can be hidden as "natural flavors" not risking it. Keep it simple.
Enjoyed the outing with no food hangover so to speak. Yippeee.

domain: food


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Chicken Stir Fry served with fresh mixed vegetables
Shrimp and Crab Cakes served with fresh sautéed Green Beans
Broccolli Stuffed Burger served with fresh sautéed mixed vegetables
Pan seared Pork Loin served with smothered Okra
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RentSkis.com Gold Club
The Rentskis.com Gold Club offers premier guest service and ski and snowboard rentals in a personal setting. All new gear every year. Complimentary overnight ski and boot storage with rental. Boot-drying and daytime shoe storage.
Beaver Creek Village

domain: hobby


Welcome to Nexus Audio, a recording studio located in the heart of Oakland, CA. If you want fresh sounds in this age of musical monotony, look no further. We blend technology, talent and vibe in our "homey" seven room facility. Offering Tracking, Mixing and Mastering, as well as a fully equipped rehearsal space for those important short-notice practices.
Need more spice to your music? Our elite in-house studio musicians will make any of your musical visions a reality. We utilize tasty analog preamps and com- pressors including Universal Audio, Neotek, and Trident. Our extensive mic cabinet has over 30 microphones to try out. We run ProTools HD 8 and use 192 bit convertors to ensure high quality Analog–Digital conversion. If you are interested in our services or want more information feel free to e-mail or call to
schedule a session, or free consultation in the studio about your project.
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Time complexity of algorithmcode is not equal to the actual time required to execute a particular code but the number of times a statement executes. Bigo algorithm complexity cheat sheet know thy complexities. Most computers offer interesting relations between time and space complexity. The natural variability of streamflow is being complicated by anthropogenic influences and climate change, which may introduce additional complexity into streamflow records. On the complexity of maintaining partial sums siam. Time complexity of an algorithm signifies the total time required by the program to run till its completion. Function point concentrates on functionality provided by the system. Systolic arrays free download as powerpoint presentation. Understanding time complexity with simple examples. Spacetime, in physical science, single concept that recognizes the union of space and time, first proposed by the mathematician hermann minkowski in 1908 as a way to reformulate albert einsteins special theory of relativity 1905. We consider an example to understand the complexity an algorithm. One of the earliest theorem related to space complexity is savitchs theorem. Complexity analysis department of computer science.
Hence the space complexity for the above algorithm is o4 o1. All books are in clear copy here, and all files are secure so dont worry about it. However, we dont consider any of these factors while analyzing the algorithm. For time complexity, such a simulation seems to require an exponential increase in time. An informal analogy would be the amount of scratch paper needed while working out a problem with pen and paper. While the design and analysis of algorithms puts upper bounds on such amounts, computational complexity theory is mostly concerned with lower bounds. Similarly, space complexity of an algorithm quantifies the amount of space or memory taken by an algorithm to run as a function of the length of the input. Data structures time and space complexity flashcards quizlet. We provide an oraclerelativized proof of the separation, and of a lower bound on space for lineartime reversible simulations. Amortized analysis guarantees the average performance of each operation in the worst case.
Complexity, time and music article pdf available in advances in complex systems 2. The time complexity is a function that gives the amount of time required by an algorithm to run to completion. Spacetime, in physical science, single concept that recognizes the union of space and time, first proposed by the mathematician hermann minkowski in 1908 as a way to reformulate albert einstein s special theory of relativity 1905. In this section we will look at the problem of how much space andor time it takes to solve certain decision problems, and whether there are space and time hierarchies of decision problems. Space complexity memory limits provide information about the expected space complexity. The class pspace is the set of all languages that are decidable by a tm running in polynomial space. Auxiliary space is the extra space or temporary space used by an algorithm. According to this theorem, a deterministic machine can simulate nondeterministic machines by using a small amount of space. For the add, time and space complexity may be o1 if just appends its argument to the expconcepts array front index. It is the function defined by the maximum amount of time needed by an algorithm for an input of size n. Physics of the impossible free download pdf download. Additionally, lets say that traversal algorithm needs not 1 but pointers, the. The amount of time needed by a program to complete its execution is known as time complexity. This measurement is extremely useful in some kinds of programming evaluations as engineers, coders and other scientists look at how a.
Following are the correct definitions of auxiliary space and space complexity. We will only consider the execution time of an algorithm. Or we might say this algorithm takes constant extra space, because the amount of extra memory. Step count method to measure time complexity of an algorithm part 1 duration. Space complexity is more tricky to calculate than time complexity. Study participants consisted of engineers and managers at applied physics laboratory, goddard space flight center, jet propulsion laboratory, johnson space center, and marshall space flight center. Instant access to millions of titles from our library and its free to try. Jun 06, 2017 space and time complexity of an algorithm duration. The first order of functions, o1, or constant time complexity, signifies that the algorithms running time is independent of the input size and is the most efficient. Complexity can be viewed as the maximum number of primitive operations that a program. Use of time complexity makes it easy to estimate the running time of a program. Systolic arrays time complexity matrix mathematics.
Start studying data structures time and space complexity. This book defines, describes, and prescribe the newly emerged paradigm of complexity of changehow a simple system ruled by a deterministic law can evolve in a manner too complex to predict in detail in the long run. May 17, 2018 just count the number of steps the program takes on input of size n. Time complexity and space complexity stack overflow. For large problem sizes the dominant termone with highest value of exponent almost completely determines the value of the complexity expression. The space complexity of a tm is the space or memory taken as a function of the input length n in the worst case. Thus time complexity depends on the size of the program and type of the algorithm being used. I have been searching for many websites that contain information of the space complexity of java data structures. Time and space complexitytime complexitythe total number of steps involved in a solution to solve a problem is the function of the size of theproblem, which is the measure of that problems time complexity. Time complexity is commonly estimated by counting the number of elementary operations performed by the algorithm, supposing that each elementary operation takes a fixed amount of time to perform. Big o notation time complexity and space complexity binary search is a technique used to search sorted data sets. Time and space complexity of this algorithm depends on implementations of expandconcept, getkeywords, add and performsearch functions.
To answer your question, if you have a traversal algorithm for traversing the list which allocate a single pointer to do so, the traversal algorithms is considered to be of o1 space complexity. We have only been talking about running timespeed so far. The study adopted a simple definition of complexity. We will study about it in detail in the next tutorial. An abstract to calculate big o factors of time and space complexity of machine. In computer science, the space complexity of an algorithm or a computer program is the amount of memory space required to solve an instance of the computational problem as a function of characteristics of the input. The measurement of time is done in terms of number of instructions executed by the program during its execution. How to find the space and time complexity using the bigo.
The time complexity of algorithms is most commonly expressed using the big o notation. Jorgensen, cyclomatic complexity of a module should not exceed 10. In this paper, we discuss a number of results around fast exponential time algorithms that come with exponential space complexities. They are just approximations, and will vary depending on the speci. Python time and space complexity hello, i have implemented 5 different functions which i list here and i want to calculate the time and space complexity together with the worst case complexities. Modern complexity theory is the result of research activities. For some of the problems, this goal has not been realized yet. Pdf an abstract to calculate big o factors of time and space. Examples of languages in pspace include allre and any contextsensitive language. After explaining, through examles, the underlying idea of sensitive depenence on. The complexity of an algorithm is simply the amount of work the algorithm performs to complete its task. I need to know how to find time complexity for this small piece of code written, and if possible how to optimize it. Similar to time complexity, space complexity is often expressed asymptotically in big o. Computer science stack exchange is a question and answer site for students, researchers and practitioners of computer science.
Its beginnings can be traced way back in history to the use of asymptotic complexity and reducibility by the babylonians. Time complexity measures the amount of work done by the algorithm during solving the problem in the way which is independent on the implementation and particular input data. This webpage covers the space and time bigo complexities of common algorithms used in computer science. In theoretical analysis of algorithms it is common to estimate their complexity in.
The term space complexity is misused for auxiliary space at many places. The other onotations are listed in their rank order of efficiency. The space complexity of the algorithm is just the added space complexity of all the data structures it needs to have going at any given time. The averagecase running time of an algorithm is an estimate of the running time for an average input. In computer science, the time complexity is the computational complexity that describes the amount of time it takes to run an algorithm. I am searching specifically for the space complexity of the hashmap, arraylist, stack and linkedlist. What have i tried i have done some reading on the internet about how to calculate time and space complexity of an algorithm, and below is what i feel is the answer, but since i am new to this i thought rather than going with a. This measurement is extremely useful in some kinds of programming evaluations as engineers, coders and other scientists look at how a particular algorithm works. Space complexity shares many of the features of time complexity and serves as a further way of classifying problems according to their computational difficulties. Shows bigo time and space complexities of common algorithms used in. The following is also quadratic time, just like a quadratic expression in algebra.
You can see which collection type or sorting algorithm to use at a glance to write the most efficient code. Time and space complexity depends on lots of things like hardware, operating system, processors, etc. Big o notation time complexity and space complexity. Big o notation fn ogn means there are positive constants c and k such that. Time complexity of an algorithm represents the amount of time required by the algorithm to run to completion. It works by selecting the middle element of the data set, essentially the median, and compares it against a target value.
It is the time required to perform a sequence of related operations is averaged over all the operations performed. Has lower time complexity of classification than comparable software due to inference based on sparse model representation and use of an inverted index. Its an asymptotic notation to represent the time complexity. It is the minimum amount of time that an algorithm requires for an input of size n. In this section we will look at the problem of how much space and or time it takes to solve certain decision problems, and whether there are space and time hierarchies of decision problems. Space complexity is a function describing the amount of memory space an algorithm takes in terms of the amount of input to the algorithm.
Read online and download ebook strategic management and organisational dynamics. Space complexity in algorithm development is a metric for how much storage space the algorithm needs in relation to its inputs. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Ebook earth s proximal space as pdf download portable. Space complexity is sometimes ignored because the space used is minimal and or obvious, but sometimes it becomes as important an issue as time. I am creating a website my academic project in which user can upload his program files. It also makes good sense to talk about the complexity of other things. Space complexity of an algorithm is total space taken by the algorithm with respect to the input size. Algorithms and data structures complexity of algorithms. For example, we might say this algorithm takes n 2 time, where n is the number of items in the input. Pdf the complexity of geodesic motion in general spacetimes. Time and space complexity analysis of recursive programs using factorial duration. It is the memory required by an algorithm to execute a program and produce output. Is there any code in java for finding complexity of a program.1370 1375 1165 365 899 239 1194 908 732 99 69 871 660 433 940 947 1068 265 31 1106 1104 1085 1088 663 1483 368 475 136 1337 481 1275 1089


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Avenged Sevenfold have announced that they’ll release the Black Reign EP this coming Friday (September 21) via Warner Bros Records.
It’ll feature all four of their tracks that have been in Treyarch’s Call Of Duty: Black Ops video game series – including new single Mad Hatter, which launched yesterday and will appear on Call Of Duty: Black Ops 4, out on PlayStation 4, Xbox One and PC on October 12.
It’ll be joined on the digital EP by Not Ready To Die from Call Of Duty: Black Ops, Carry On from Call Of Duty: Black Ops II and Jade Helm from Call Of Duty: Black Ops III.
A7X vocalist M Shadows says: “Black Ops 4 looks insane and is something completely new for fans, so we felt that we should take a similar leap with the music and go for something bigger, darker and more cerebral.”
As for the inspiration behind Mad Hatter, Shadows adds: “Watching the initial trailers and looking at production sketches reminded me of the S-Town podcast and its main protagonist, John B. McLemore, who was rumoured to have suffered from mercury poisoning, or Mad Hatter disease.
“The idea of Mad Hatter and what it does to the brain is as frightening as the images we were shown. So I decided that the lyrics would shadow McLemore’s life. The result is a thick-grooved song that’s dynamic and has a weightiness to it.”
Shadows continues: “We have a very deep relationship with the team at Treyarch. We’re proud to be part of the family and extremely excited for people to enjoy this next chapter and our contribution to it.”
News of the band working on material for Call Of Duty: Black Ops 4 first came to light earlier this year.
<a href="https://target.georiot.com/Proxy.ashx?tsid=38569&GR_URL=https%3A%2F%2Famazon.co.uk%2FCall-Duty-Black-Ops-Xbox%2Fdp%2FB07BB4QXNV%3Ftag%3Dhawk-future-21%26ascsubtag%3Dhawk-custom-tracking-21" data-link-merchant="Amazon UK"" rel="nofollow" target="_blank">Call Of Duty: Black Ops 4
The latest instalment in Activision's smash hit Call Of Duty series is set to fire its way on to PlayStation 4, Xbox One and PC in October. Don't miss out of what will be yet another explosive experience.

domain: entertainment


USPS envisions a CMS for complaints
The U.S. Postal Service has put out feelers for a web-based system to manage all of the documents and records that come in over the agency hotline, including those related to criminal allegations and investigations.
The agency is aiming high. In a recent request for information, USPS listed features it was seeking for the complaint management system. Files need to be securely stored and access strictly controlled based on user profiles.
USPS also wants the system to be “highly intuitive and configurable by the user,” allowing for easy navigation from case to case or record to record, as well as throughout various sections within each digital case file. The system should also create configurable, unique identifying numbers to each allegation, investigation and hotline complaint.
The needs and requirements for managers and supervisors also need to be addressed so that they can manage and report on the work done by agents in the field. Besides offering an “extensive, user configurable system of approvals, workflows and tracking processes for the various elements within an investigation,” the system should be able to assign investigative responsibility by program area for field investigators and be capable of alerting individuals, via e-mails or personalized dashboards, that an event requires their attention.
For analytics, USPS expects the system to provide dynamic, real-time dashboards and reports depicting the status of various approvals, results, data points and pending items for current investigations and for trend analysis. It would also take advantage of API technology to interface with other analytics products throughout the enterprise network.
Responses are due Nov. 7, 2014.
Posted by GCN Staff on Oct 28, 2014 at 1:07 PM


On Monday, President Trump said he is planning to meet with officials in the vaping industry to “come up with an acceptable solution to the Vaping and E-cigarette dilemma.” This comes after signaling last Friday that his administration was planning to significantly restrict vaping products. These possible restrictions ranged from not allowing people to buy vapes until they are 21 to outright banning all flavored vapes.
Considering so much of the rhetoric surrounding this vaping “epidemic” has been related to young people, I think the fact that I am a high school student gives me a valuable perspective. I do not vape; I never have and do not plan on it in the future.
With that said, I also think the Trump administration is going in the completely wrong direction on this issue. As I see it, there are a few central reasons why the Trump administration’s nanny-state anti-vaping agenda is both nonsensical and counterproductive.
First, the way President Trump seems to be making his stance more palatable to the masses is by saying that the reason he must restrict people’s ability to vape is that it is important to “protect the kids.” There are two fallacies in this argument.
First, he is ignoring that the damage vaping has done is minuscule in the big picture. Since the beginning of the vaping “epidemic,” 33 people have died. That is obviously far too many, but to say that we should severely restrict vaping for this reason but not tobacco or alcohol, which kill 8.5 million people each year worldwide, is insane.
Not to mention that many states are not only in the process of legalizing marijuana but also decriminalizing harder drugs. This is not to say that we should criminalize any of these substances, but it is to say that it does not make sense why President Trump sees vaping as a unique evil that deserves to be disproportionately regulated while his administration is not instead focusing on much bigger problems.
The second issue with his “protect the kids” defense is that President Trump fails to acknowledge the real reason people have been dying from vaping. The reason is not that they are using legally bought vapes, but because they are ingesting chemicals that are only in vapes found on the black market. NBC News says, “There’s now solid evidence that vitamin E oil found in bootleg THC vape products is behind at least some of the 2,000-plus severe vaping-related lung injuries nationwide.”
The keyword there is “bootleg.” If President Trump truly wants to prevent young people from falling ill as a result of vaping, then he would be working to ensure people are buying legally. He would not be pushing them into the black market, where they will be more likely to get vapes laced with vitamin E acetate. Raising the age to buy vapes to 21 pushes everybody under that age to buy the very vapes that pose the most serious health risks.
The second main reason the restrictions being pushed by the Trump administration are misguided is that to enact the sorts of restrictions they are pursuing would ignore basic conservative principles. Government has no place in regulating the behavior of legal adults as long as that behavior is not infringing on the rights of anybody else.
To say that an 18-year-old—somebody who is a legal adult in the United States—is not permitted to use a product that may have adverse health risks simply because the government says so is absurd. One of the central tenets of freedom is the idea that each person should be free to make whatever decisions he pleases as long as it does not harm anybody else. Instead, the Trump administration seems to believe that it is their duty to protect people from themselves. Yet the job of the government is to protect me from other people, not to protect me from my own poor decision making.
There is no doubt that too many young people are using vapes, but to say that any of the regulations I discussed above are the be-all-end-all solution would be to have a misinformed view of the real issue. If we really want to curb the use of vapes among young people, then I would look to solutions that do not include government compulsion under the thin veil of just wanting to “protect the children.”


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Horse of the Month October 2022
We’re delighted to introduce our Horse of the Month for October…the gorgeous Red! Red is a 28 year old Dartmoor pony who has been with her owner Libby since she was 6 years old!
Two years ago, Red got laminitis for the first time so her vet carried out a blood test which showed that she had Cushing’s disease. There are many factors which can affect whether a horse or pony develops laminitis, so it makes sense to control as many of them as possible to reduce the risk of horses and ponies developing this debilitating condition. Equine Cushing’s disease is one of the laminitis risk factors that you can control with medication, so Red has been prescribed a daily tablet to control her Cushing’s.
Since starting the medication Libby says that Red is looking ‘exceptionally well’ – we hope that she continues to stay happy and comfortable! Thanks for sharing her story with us Libby!

domain: medical


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Does music have any practical value? Yes. Since everything in the universe—including you—is set to varying musical vibrations, the right music can bring well-being and balance to your body, mind, and soul. God’s Presence within you knows what music is best for you, and can guide you to it.
For everything that “ails” you there is a cure—or at least a cure that is yet to be discovered. The truth is that God has provided for everyone. It’s just a matter of locating God’s naturally provided cure. So never give up on your health concerns—whether they are physical, mental, emotional, or spiritual.
What to know how to improve your life? First, turn your personal ego over to God’s Presence and Will daily, because it is your ego that is keeping you stuck where you are. Then, just let your life happen. Force nothing. Remember, it is up to God’s Presence to move you from point A to point B.
Part of living a happy and successful life is based on the kind of people who surround you. They may be negative and destructive, or they may be positive and happy. Either way, they are a reflection of your inner being. Release your ego to God’s Presence within you, and you will attract the latter.
How valuable is your presence on earth? The truth is, your value is based on how much value you are to others. That is, it is measured by how present you are to others, and what you bring into their lives, whether it is emotional, spiritual, or material support; love or caring; or even laughter.
A beautiful person is one who outwardly reflects the beauty of God’s Presence within them. What is interesting is that the part of you that is able to appreciate the beauty of another is the part of you that is One with that same beautiful Presence of God. So it takes your inner beauty to appreciate their outer beauty.
When you have a question about something that is troubling you, what’s important is what you think about it, not what others think. This is because every soul is unique, and the right answer for one person may be wrong for another. The best answers, of course, come from God’s Presence within you.

domain: psychology


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A true minimally invasive discectomy procedure extends beyond surgical access and allows for native tissue conservation where it may benefit the patient. Performing a discectomy that results in an open defect has been correlated with a higher risk of reherniation.4 Surgeons who use a tissue sparing approach while minimizing the residual defects have seen improvements in procedural outcomes. 18
The AnchorKnot® Tissue Approximation Kit was developed to augment the existing standard of care for herniated disc repair procedures. The AnchorKnot® Tissue Approximation Kit may be considered for patients undergoing herniated disc repair procedures if the surgeon identifies that the tissue is amenable to repair. This procedure may not be appropriate for all patients, and not all patients may benefit.
Features of the AnchorKnot® Tissue Approximation Kit:
Compatible with minimally invasive surgical option for suitable patients
Facilitates suture access in difficult to reach locations
Can be used in herniated disc repair procedures aimed at disc preservation


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- Number of Players
- Adventure, Arcade, Platformer
- Release Date
- 7th May 2020, $9.99
- 7th May 2020, £7.99
- Official Site
Mini Review Fledgling Heroes - A Fun And Fleeting Flappy Bird-Alike
Cast your mind back to May 2013. Wherever you were, whatever you were doing, chances are that a certain mobile game called Flappy Bird entered your life at some point after its launch. Its insanely addictive nature and the ensuing mania ultimately resulted in its removal from mobile stores, but there’s no denying that its simple...
About The Game
Leap from the nest into a fun-filled adventure! Meet cute feathered friends, each with their own wondrous story to tell, as they dodge dastardly pirate lizARRrds, dash through frozen forests, dive under sunken temples, and flap their way from fledglings to a fully-fledged heroes!
- It’s dangerous to go alone - play with a friend! Drop in another player at any time for some cooperative assistance… or some competitive capers.
- Flex your creative muscles and make your very own levels with the built-in editor. Share them with your friends and the world!
- Customise your cute birds with wacky colours, or recreate your favourite variations found in the wild.
- Explore 90+ hand-crafted levels set in gorgeously unique and colourful environments.
- Compete for the fastest time in race levels, charge through breakneck-paced endless levels, and go head-to-head with dragons!
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domain: game


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Thanks to everyone who joined Erin Kelly, Melanie McGrath and me at Mansfield Central Library on Saturday 25 February. We had a panel discussion and Q&A, ...
Saturday, 27 February 2010
One Flew Over the Stock Exchange
At a time when bankers and brokers are pilloried in the media for turning the economy into a gibbering basket-case, the idea that a bunch of amateur thespians from the Stock Exchange should get together to perform the inmates-take-over-the-asylum piece ‘One Flew Over the Cuckoo’s Nest’ is so delicious that it’s a must-see.
The multiple layers of irony positively invite you to make up your own jokes, which is just as well since the script by Dale Wasserman, written thirteen years before the movie is flatter and flabbier than the Jack Nicholson cracker.
Amateur Dramatics in central London is an invidious occupation: there’s so much brilliant professional fringe theatre available for a tenner, that amateurs have to work twice as hard to gain their audiences. But when amateur groups are as good as SEDOS, the lines are blurred and there’s nothing in this production to suggest it’s any the less: production values, inventive lighting and sound, original music, and careful casting are entirely professional in their approach.
Director Rebecca Smith deliberately slants the production to concentrate on the inmates, even bringing in a consultant psychiatrist to help research the characters and most of these are meticulously observed cameos, with Darren Hannant specially strong as the rictus-wearing indecisive Cheswick, and Ben Hale as the tenderly vulnerable Billy Bibbett.
There’s a slight lack of pace which could pick up during the run, although it may be deliberate as in the measured delivery of Lisa Jedan’s coolly-surfaced Nurse Ratched. The ‘star turn’ should be the rambunctious prison-transferee R P McMurphy as immortalised by Nicholson, and more recently by Christian Slater in the sparky 2004 production at the Gielgud. In those shoes, Liam Byrne has a tough task to make RP an explosive mix of delightful and dangerous, and it’s not quite a bullseye. The fault’s more in the dated writing than the intensity of his performance and the wise-cracks don’t seem so sharp any more.
Finally, a plea to the otherwise wonderful Bridewell, and fringe theatres across London to invest in a couple of fifty-quid silent ceiling fans to stop the audience melting on our rare tropical evenings.
Photo by Nick Chronnell


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Solid Third Quarter Results
The company reported its Q3 results on October 14. Profit for the quarter was $1.32 billion up from $357 million a year earlier. Earnings per share surged to $1.65 from $0.42 for the same quarter a year ago, thanks mainly to low fuel prices. Revenues decreased 0.6% year over year mainly due to adverse foreign currency movements.
The company plans to expand its domestic capacity this year but continue shrinking its international capacity in response to falling demand and currency headwinds in Europe and Asia. Overall capacity growth is expected to be modest in 2016.
Analysts have raised their estimates for the company in the past few weeks after strong results. Zacks Consensus Estimates for the next year has jumped to $6.27 per share, from 5.72 per share, before the results. The company, which reports Q4 earnings next week, has an excellent history of beating estimates as can be seen from the following chart:
Delta Beats United to Become Number 2 Airline by Traffic
Earlier this week, the airline reported 209.6 billion miles flown by paying passengers during 2015, up 3.3% year-over-year, and surpassed United to become the #2 airline in the nation by traffic. American Airlines remains at the top spot and Southwest at the fourth. These top four airlines account for 80% of the domestic traffic in the US.
Ranked #3 in 2015 Airline Scorecard
Delta scored an overall rank of 3 in the annual ranking of major airlines in operational areas by Middle Seat Scorecard. Smaller airlines—Alaska and Virgin America—occupied the top spots, while Delta’s larger peers–United and American–were at #7 and #9 respectively.
Excellent Growth Potential with Attractive Valuation
In addition to a top Zacks rank, the stock has a Style Score of “A” for both Value and Momentum and “B” for Growth. It is currently trading at an attractive multiple of 7.5 times forward earnings.
Additionally the Airline industry is currently ranked 11 out of 265 Zacks industries (top 4%), reflecting solid fundamentals for the industry.
The Bottom Line
Delta is undoubtedly one of the best run airlines in the country. The airline will continue to benefit from improving economy and growing demand for air travel, in addition to tailwinds from low fuel prices. While many of its international markets are not doing well as of now, its international presence and partnerships enhances longer-term growth potential.
Want the latest recommendations from Zacks Investment Research? Today, you can download7 Best Stocks for the Next 30 Days.Click to get this free report >>

domain: news


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Fuentes hurt after his vehicle collided with SUV on Highway 40 near 40th Avenue
GRACE TOWNSHIP, MN, (March 10, 2021) - A two-vehicle accident on Wednesday, March 10th on Highway 40 in Grace Township resulted in 18-year-old Alex Steven Fuentes being injured.
A two-vehicle accident on Wednesday, March 10th on Highway 40 in Grace Township resulted in 18-year-old Alex Steven Fuentes being injured.
The accident occurred at about 6:50 p.m., according to the Minnesota State Patrol (MSP), when Fuentes’ Nissan Rogue collided with a Jeep Cherokee driven by 21-year-old Victoria Lynn Pagel on Highway 40 near 40th Avenue. Fuentes was taken to Willmar Hospital for treatment of his injuries.
The MSP is investigating the accident.
TWO-VEHICLE CRASH INVESTIGATION
During its investigation, the MSP will try to determine what caused the crash that resulted in Mr. Fuentes being injured.
Several factors can play a role in accidents, including but not limited to impairment, reckless driving, distracted driving, road conditions and speeding. Candice Bond, an experienced accident attorney, said that in cases such as this it is imperative to have an independent investigation conducted by a seasoned lawyer as soon as possible to preserve evidence for an injured victim.
“In addition to the diligent work of law enforcement, an attorney can re-interview important witnesses, analyze any surveillance video from the scene of the crash and obtain phone and texting records of the liable party,” said Bond, Managing Partner of Bond Legal and national analyst for The Legal Advocate. “Serving as an advocate, an attorney can help compassionately guide a victim and their family during this difficult time, vigorously protecting their legal rights and best interests.’’
PERSONAL INJURY CLAIM
Bond noted that it is vital for victims to hire an attorney when filing a personal injury claim, which allows them to negotiate with an insurance company on behalf of a client.
“Insurance companies often try to pressure victims to settle for a quick payout for far less than the case is worth,” Bond added. “They are financially motivated to limit compensation and do not factor in the long-term consequences and suffering that a victim will ultimately experience. Attorneys vigorously advocate for their clients to ensure that they are treated with respect and compensated fairly.”
The Legal Advocate's national legal analyst Candice Bond is experienced in serious injury auto accidents, and if you or a family member need help she's available for a free consultation to guide you
(Candice Bond, our national legal analyst, is a Managing Partner at Bond Legal. The Legal Advocate can and does use quotes from prior interviews with our analysts for our news articles. If you would like to contact Bond Legal to help you, please call 866-599-0297 or use the submission box found on this page. Legal analysts quoted may or may not be licensed in your state).


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No, cats should not eat chicken nuggets because they can be harmful to their health. Chicken nuggets may contain additives, seasonings, and oils that can upset a cat’s digestive system and lead to health issues like pancreatitis or obesity.
Introducing human food into a cat’s diet can be risky. While cats are obligate carnivores and require a meat-based diet, it is essential to feed them a balanced and species-appropriate diet specifically formulated for their nutritional needs. Chicken nuggets are processed and often high in fat and sodium, which can cause digestion problems and potentially contribute to long-term health problems in cats.
To ensure your cat’s well-being, it is best to stick to cat-specific food, which can provide them with the necessary nutrients in a safe and balanced way.
Why Chicken Nuggets Are Appealing To Cats
Cats are naturally drawn to the crispy texture and enticing aroma of chicken nuggets. They have a preference for high-protein diets due to their instincts for meat-based foods. Their attraction to these nuggets is not surprising, considering their carnivorous nature.
However, it is important to note that while cats may enjoy the taste of chicken nuggets, they should not be a regular part of their diet. These processed foods are often high in salt, additives, and unhealthy fats, which can be harmful to feline health.
Cats should primarily consume a balanced diet of quality cat food that meets their nutritional needs. Feeding them chicken nuggets can lead to nutritional imbalances and potential health issues. It is crucial to prioritize the well-being of our furry friends and provide them with appropriate and healthy food options.
Potential Risks Of Feeding Chicken Nuggets To Cats
Feeding chicken nuggets to cats can pose potential risks to their health. High sodium content in these nuggets can have a negative impact on feline well-being. Additionally, the artificial additives and preservatives present in chicken nuggets may not be suitable for cats.
These ingredients can lead to gastrointestinal issues and allergic reactions in our feline friends. Therefore, it is important to avoid giving chicken nuggets to cats and instead provide them with a balanced diet specifically formulated for their nutritional needs. It’s crucial to prioritize their health and ensure they are consuming food that is safe and beneficial for them.
So, as cat owners, let’s be mindful of what we feed our feline companions and make their well-being our top priority.
Safe Alternatives To Chicken Nuggets
Chicken nuggets can be harmful to cats due to their high salt and seasoning content. However, there are safe alternatives available. Cooked, unseasoned chicken is a healthier option that cats can enjoy. Specially formulated cat treats that mimic chicken flavor can also be a good choice.
If you prefer making homemade treats, there are cat-friendly chicken nugget recipes available that are safe for your feline friend. It’s important to remember that cats have specific dietary needs, so it’s best to consult with a veterinarian before introducing any new foods.
Providing a balanced and nutritious diet is crucial for your cat’s overall health and well-being.
Frequently Asked Questions Of Can Cats Eat Chicken Nuggets
Can Cats Eat Chicken Nuggets?
Cats should not eat chicken nuggets as they can be harmful to their health. Chicken nuggets are often cooked with seasonings, salt, and additives that can be toxic for cats. Additionally, the breading on the nuggets can cause digestive issues and obesity in cats.
It’s best to stick to a balanced diet specifically formulated for felines.
While cats may enjoy the taste of chicken nuggets, it is not recommended to feed them this type of food. Chicken nuggets are typically high in salt, spices, and preservatives, which can be harmful to cats’ delicate digestive systems. Cats require a diet that is specifically formulated to meet their nutritional needs, which includes a balance of protein, fats, carbohydrates, vitamins, and minerals.
Offering a well-balanced and species-appropriate diet, such as high-quality cat food, is essential for their overall health and well-being. If you are looking to give your cat a special treat, it’s better to opt for cooked, plain chicken without any seasoning or added ingredients.
Remember to consult with your veterinarian about any dietary concerns or questions you may have regarding your cat’s food choices. By prioritizing their nutrition, you can ensure they lead a long and healthy life.

domain: pet


Top Gear said yesterday it was the most important vehicle at the 2014 North American International Auto Show in Detroit. Yes, the new Ford F-150 has been labeled as superior to the likes of the Toyota FT-1 concept and 2014 911 Targa we covered yesterday.
Are those blokes crazy, or do they make a good point?
Well, they’re crazy, of course. But they make a heckuva good case while basking in the crazy.
Their case is justified by saying the F-150 is:
Destined to be, like its predecessors, the biggest-selling vehicle in America. It has been that way for the past 32 years on the trot. They’ve now made 30 million of these things over the generations, which makes the Beetle or Toyota Corolla look, well, a bit niche.
So, while we could spend time talking about the new F-150’s 700-pound weight loss, its aluminum body or the new EcoBoost 2.7-liter V6 engine, the most interesting change is the disappearance of something great.
It’s with a heavy heart that I read that Ford’s bad-boy 6.2-liter V8 has not been carried over, replaced instead by a 3.5-liter EcoBoost V6. It’s a sad day in truck-dom indeed.
Top Gear is right in saying this new truck, with its military-grade aluminum body and high-strength steel skeleton will surely continue Ford’s 30+-year winning streak, I’m more inclined to see the value side of all this. As cool and strong as this new truck appears, there are 30 million used trucks out there, all of them carrying the DNA of what makes the F-150 great.
Instead of jumping in line for a brand new 2015 truck, why not search for a killer deal on a used 2010–12 model? Get all that F-150 toughness while saving money and, even better, having the option of owning one of the last 6.2-liter F-150s Ford ever made.
Would you take a chance on the new F-150, or get a used one instead?


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If you're in the market for a new home, you'll most likely need a mortgage in order to afford it. But for some home buyers, getting a mortgage isn't easy. Here are three factors that can influence the likelihood of approval on the second try.
If you're looking into fixed term mortgages, you might be wondering whether there's any reason why you should take the full term to pay off the loan. In a lot of cases, paying off a mortgage before it comes due is a great decision. If you're considering paying off your mortgage early, you'll experience a variety of benefits - here are just a few of them.
Are you thinking about using a mortgage to buy a new home? In this blog post we'll discuss mortgage calculators and how to use one of these tools to determine your monthly mortgage payments, interest charges, amortization periods and more.

domain: finance


text: Results 1–10 of 25 for "Children's Book"X related to "PCORI seeks health care stakeholders to..." Refine Your Search Refine Your Search TopicChildren (18)Emotional health (6)Teens (5)ADHD (4)Disability (3) 9 more... [+] Education (3)Learning & memory (2)Parenting (2)Anger (1)Bullying (1)Death & dying (1)Personality (1)Sport & exercise (1)Stress (1)Hide detailsDocument TypeChildren's BookXYear2014 (5)2013 (3)2012 (7)2011 (7)2010 (3)Author/ContributorQuinn, Patricia O. (3)Adinolfi, JoAnn (1)Annunziata, Jane (1)Arbona, Marion (1)Barber, Beth (1) 41 more... [+] Battuz, Christine (1)Ben-Ami, Uzi (1)Bisaillon, Josée (1)Bone, Jeffrey (1)Bone, Lisa (1)Craver, Marcella Marino (1)DeLuca-Acconi, Robin (1)Docampo, Valeria (1)Greves, Julie (1)Hood, Korey K. (1)Ische, Bryan (1)Keay, Claire (1)Kim, Cecil (1)Kim, Joo-Kyung (1)Kim, Young-Ah (1)Kimbell, Sandra (1)Kraus, Jeanne (1)Lamia, Mary C. (1)Lemniscates, (1)Levins, Sandra (1)Maitland, Theresa E. Laurie (1)Mangan, Tricia (1)Meens, Estelle (1)Moss, Wendy L. (1)Neimark, Jill (1)Nemiroff, Marc (1)Pearce, Carl (1)Peterkin, Allan (1)Pidgen, Emmeline (1)Pinelli, Amerigo (1)Poulin, Andree (1)Shin, Ji-Soo (1)Simmel, Mike (1)Skead, Robert (1)Stern, Judith (1)Stern, Judith M. (1)Tenhulzen, Katy (1)Tibo, Gilles (1)Weiner, Marcella Bakur (1)Wenisch, Tanja (1)Wilkinson, Fred (1)Hide details Results 1–10 of 25 Previous 1 2 3 ... Next Relevance Title A-Z Title Z-A Newest First Oldest First Sort by: 1.Putting on the Brakes: Understanding and Taking Control of Your ADD or ADHD, Third EditionNow in its 20th year of publication, Putting on the Brakes remains the essential go-to resource for kids, parents, and professionals looking for tips and techniques on managing attention disorders.Children's Book (April 2012)2.School Made Easier: A Kid's Guide to Study Strategies and Anxiety-Busting ToolsThis book helps kids learn some ways to reduce their anxiety, increase their confidence in school, and study more effectively.Children's Book (August 2013)3.Get Ready for Jetty!: My Journal About ADHD and MeIn this book, 4th grader Jetty writes about school, her likes and dislikes, and her recent ADHD diagnosis in her diary.Children's Book (September 2012)4.Ready for Take-Off: Preparing Your Teen With ADHD or LD for CollegeThis book lays out a plan to keep students with ADHD or LD in college by first teaching parents to prepare their teen for take-off and their first solo flight away from the home.Children's Book (November 2010)5.The Magic ClotheslineWhen Robin's father leaves for a long business trip, Robin is overcome by sadness - until gifts start showing up on the clothesline outside his house that only his father would know to give!Children's Book (September 2012)6.AD/HD and the College Student: The Everything Guide to Your Most Urgent QuestionsThis book answers the most common questions and tackles the most challenging problems students with AD/HD face in college.Children's Book (May 2012)7.A Happy HatThis is a story of resilience, optimism, and hope. The life story of a hat and its various owners illustrates how dealing with disappointments and stressful situations is crucial to one's well-being.Children's Book (September 2013)8.Mighty Mike Bounces Back: A Boy's Life With EpilepsyCo-written by a real-life basketball star with epilepsy, this empowering story helps kids learn to live comfortably with their epilepsy and to take control of their life.Children's Book (September 2011)9.Chillax!: How Ernie Learns to Chill Out, Relax, and Take Charge of His AngerThis book for kids ages 8–13 presents information on anger and body signals and offers six strategies to keep calm and retrain their brains to be more content.Children's Book (August 2011)10.Type 1 Teens: A Guide to Managing Your Life With DiabetesThis book gives teens a slew of strategies and tips to manage their day-to-day lives with type 1 diabetes.Children's Book (June 2010) Previous 1 2 3 ... Next Relevance Title A-Z Title Z-A Newest First Oldest First Sort by: ADVERTISEMENT Results 1–10 of 25 for "Children's Book"X related to "PCORI seeks health care stakeholders to..."

domain: library


Album “Homeland” appeared on the Internet on September 14.
It includes 12 songs such as “Homeland”, “Harry”, “Guerilla-rock”, and new songs. “Sacred fire” of Lyapis Trubetskoj is in a new album.
The next concert is to be held in Warsaw on October 4. The rock band will perform new songs. Those Belarusians intending to the concert cam get a free visa. You can apply by reference
It should be recalled that Siarhei Mikhalok and his band are not allowed to perform.


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For All Skin Types. 4 oz. Less fluff AND more hydration power!! How can we make the best cleanser better? We removed 5 synthetic preservatives (including silicone) & added moisture power, more anti-inflammatory, and anti-bacterial plant based ingredients!
Gently cleanse without doing away with skin’s essential oils with this sulfate-free cleanser. Moisture rich Hyaluronic Acid, Aloe, and Glycerine with the hydration retention power of Vitamin E leave skin quenched and supple. Acne and rosacea prone skin and new repaired skin cells love the calming effects of Cucumber and Calendula. Galangal Root Extract along with Palmitoyl Glycine prevent acne & irritation with their anti-inflammatory and anti-bacterial properties. Palmitoyl Glycine also reduces lines and wrinkles by encouraging collagen formation.

domain: chemistry


The taxi route for commercial jets at Blue Grass Airport was altered a week before Comair Flight 5191 took the wrong runway and crashed, killing all but one of the 50 people aboard, the airport’s director said Monday.
Both the old and new taxiways to reach the main commercial runway cross over the shorter general aviation runway, where the commuter jet tried to take off early Sunday, Airport Executive Director Michael Gobb told The Associated Press.
While the main strip, Runway 22, is 7,000 feet long, the shorter one, Runway 26, is just 3,500 feet. Aviation experts say the CRJ-100 would have needed 5,000 feet to fully get off the ground.
The runway repaving was completed late on the previous Sunday, one week before the crash, Gobb said.
It wasn’t clear if the Comair pilots aboard Flight 5191 had been to the airport since the changes. Comair operates that regular 6 a.m. Sunday flight to Atlanta from Lexington, but another commuter airline takes over the early morning commute during the week.
The National Transportation Safety Board is investigating the crash and said it was reviewing runway and taxiway markings as part of its investigation.
Recorded conversations between Comair Flight 5191’s cockpit crew and the single person staffing the control tower in the minutes before Sunday’s crash showed no signs of trouble. The only runway mentioned was the main commercial strip, Runway 22, said NTSB member Debbie Hersman.
Somehow, the commuter jet ended up on Runway 26 instead — a cracked surface meant for small planes that was much too short for Comair’s twin-engine jet.
What followed was the worst U.S. plane disaster since 2001.
“The take-off began, and the aircraft continued to accelerate until the recording stopped,” Hersman said.
The plane clipped trees, then quickly crashed in a field and burst into flames, killing everyone aboard but a critically injured co-pilot who was pulled from the cracked cockpit.
Preflight ‘consistent’ with regular practice
Information retrieved from the cockpit voice recorder indicated that the preflight preparations had been “consistent with normal operations,” Hersman said Monday.
There were no obvious problem with the airworthiness of the plane and the engines were in tact and appeared to have been in good working order, she said.
“Air traffic control and the flight crew planned for a takeoff from runway 22,” Hersman said. But “The F.D.R. (flight data recorder) and the evidence on scene indicates the crew took off from Runway 26.”
Lowell Wiley, a flight instructor who flies almost daily from Lexington, said he was confused by the redirected taxi route when he was with a student taking off from the main runway Friday.
Pilots encountered problems with the runway layout at Lexington’s airport in the past, as well.
In a letter filed in 1993 with the Aviation Safety Reporting System, maintained by the National Aeronautics and Space Administration, a pilot described his experience:
“Aircraft was cleared for immediate takeoff (traffic was inside the marker) on runway 22 at KLEX. We taxied onto the runway and told tower we needed a moment to check our departure routing with our weather radar (storms were in the area, raining at the airport). We realized our heading was not currect for our assigned runway and at that moment, tower called us to cancel the takeoff clearance because we were lined up on runway 26.”
Pilot suggested warning
The pilot, who is not identified, suggested the Lexington airport post a warning to pilots “to clarify multiple runway ends,” according to a text of the letter provided by FlightAware.com.
Hersman said the NTSB was interviewing the controller on duty early Sunday, reviewing records and transcribing the data and voice recorders retrieved from the crash.
Monday afternoon, investigators planned to use a high-riding truck to try to get the same view of the runway and airport layout that the pilots of Comair Flight 5191 would have had, she said.
She said they planned to conduct the same test on Tuesday at 6 a.m., the time of the crash to “try to see what the pilot saw.”
The plane’s two pilots were familiar with the twin-engine CRJ-100, and that plane in particular, the plane’s maintenance was up to date, and it wasn’t an old aircraft, Comair President Don Bornhorst said. Comair, based in Erlanger, Ky., is a subsidiary of Atlanta-based Delta Air Lines Inc.
“We are absolutely, totally committed to doing everything humanly possible to determine the cause of this accident,” Bornhorst said Sunday.
At Blue Grass Airport, flights were back to normal Monday. The 6 a.m. Lexington-to-Atlanta flight took off safely, though with a different flight number, Delta 6107.
“Obviously there is some anxiety when something like this happens, but it is not something that would stop me from going,” said Mark Carroll, 47, a computer consultant from Lexington who was boarding the flight to Atlanta. “Things happen when you get older, it happens to everyone. You keep doing what you’re doing.”
The wreckage of Flight 5191 remained largely intact but severely burned in a field about a mile away.
The burned bodies of the 49 victims were removed from the plane on Sunday and taken to the state Medical Examiner’s Office in Frankfort for autopsies to determine the cause of death. Fayette County Coroner Gary Ginn said Sunday that they likely died in the fire.
The victims included a , a director of Habitat for Humanity International, an owner of a thoroughbred horse farm, a University of Kentucky official and a Florida man who had caught an early flight home to be with his children.
Amid the devastation, there was also a story of heroism: Police Officer Bryan Jared reached into the broken cockpit and pulled out James M. Polehinke, the plane’s first officer, burning his own arms to save the man. Polehinke was listed in critical condition at University of Kentucky Hospital.
The crash marked the end of what has been called the “safest period in aviation history” in the United States. There has not been a major crash since Nov. 12, 2001, when American Airlines Flight 587 plunged into a residential neighborhood in New York City, killing 265 people, including five on the ground.


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This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029299
PrimePCR™ PreAmp for SYBR® Green Assay: PCSK4, Human
PrimePCR™ Template for SYBR® Green Assay: PCSK4, Human
This gene encodes a member of the subtilisin-like proprotein convertase family. These enzymes process latent precursor proteins into their biologically active products. The encoded protein plays a critical role in reproduction and processes multiple prohormones including pro-pituitary adenylate cyclase-activating protein (proPACAP) and pro-insulin-like growth factor II. [provided by RefSeq Feb 2012]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

domain: health


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Using genetic algorithms to evolve genes…
Promoter Evolution : Genetic Algorithms
In this article we will use promoter data from one of the previously mentioned papers by Kotopka and Smolke. First, a CNN deep learning model will be trained on the data to predict each promoter’s expression levels. Then, a genetic algorithm will be written to mutate a promoter to an expression target level.
Genetic algorithms have nothing to do with genetics, instead they take inspiration from the process of evolution; mutations, breeding, and selection in order to optimize fitness.
In the context of machine learning and computer science, these genetic algorithms are developed in order to search parameter space of a model.
For the problem of promoter design, the search space consists of all possible inputs; or all the possible sequences. For a 100 base pair sequence, there are 4^100 or 1.6 x 10^60 combinations; an unfathomably large number. Instead of testing each combination by running it through the model which may take years or decades on even the fastest computers, it would be smarter to use a heuristic to select which sequences to try. If one sequence gives a result better than random sequences; the good sequence may be slightly modified to perhaps improve it further.
The algorithm consists of themes from biology: individuals, populations, mutations, meiosis, genetic drift, and more. Individuals represent single sequences
These individuals undergo mutations, selections, and mating.
A SNP mutation is a simple base pair change, or changing one of the nucleotide indentities
There are more mutations, for example inversions (flipping consectutive base pairs), deletions (removing base pairs comepletely) or additions(adding nucleotides in the sequence). Mutations ‘explore’ the search space around a sequence, or individual in a population.
The population is made of many individuals. Additionally, these individuals can mate; which involves mixing the individuals sequences with ‘crossing over’.
Crossing over swaps out streches of the sequences between two individuals. The effect of this is to increase the diversity of the population. This is where most diversity in nature comes from; different combinations of base pairs results in most variation, mutations make up a small portion of the phenotypic diversity.
Fitness is the measure of an indivdiual’s desirability. For promoters, we may wish to create a stronger promoter to increase gene expression. Additionally, we may want to limit the number of mutations from the original sequence. Both the gene expression strength and distance can be part of an individual’s calculated fitness. Each generation, there may be a cut-off for individuals to persist in the population. Those below a cut-off for a fitness score may be discarded.
This process of mutation, mating, and selection repeats until a good enough solution is found. This is analagous to evolution in biology and works well in this case to provide a heuristic solution.
Convolution Neural Networks
Convolution Neural Networks, or CNN’s are a class of deep learning model which has been popular for tasks related to vision and text. Today, CNN’s have been the most popular tool for genetics with deep learning, however, it is beginning to replaced by other algorithms. Nonetheless, it provides a good and quick baseline for comparing models.
CNN utilize convolutional layers which work by ‘learning’ filters which correspond to features in the input text. The filters, or kernels, are a set length. The filter then moves, one character a time down the text, like a sliding window. At each step, the kernel collects the feature of the text within its window which is saved in a new version, or representation of the text. In theory, these filters will learn relevants features which enable prediction of the target task.
These convolutions over the text are often paired with another operation; pooling. Pooling effectively shrinks the dimensions of the text, thereby reducing the size of their representation and bringing together features which are distant. Eventually, the filters learn useful operations which incorporate distantly related parts of the text which are relevant for their pattern and target.
In effect, CNN’s learn features and compile information across a seqence into a smaller, more condense representation. This representation can then be used for other parts of a deep learning algorithm. The most simple model that can use CNN representations is a series of linear layers; the backbone of most deep learning tools.
Training a baseline CNN
We will use data from the Kotokpa and Smolke study which generated tens of thousands of promoter-expression pairs. They studied two promoter systems containg transcription factor binding sites, ZEP17 and GEV4, where the promoter lengths were 272 and 312 base pairs respectively. The promoters were tested under various conditions. After filtering for the highest quality datapoints with detectable expression levels, about 160,000 pairs remained.
The data was preprocessed to accommodate the model. Raw sequences were converted to numeric values which were converted to one-hot encoding [1,0,0,0] ,
Raw : (‘ATTTAGGC’)
Numeric : (‘1,2,2,2,1,4,4,3’)
One-Hot : [1 0 0 0], [0 1 0 0], [ 0 1 0 0], [0 1 0 0 ], [1 0 0 0], [0 0 0 1], [ 0 0 0 1 ], [0 0 1 0]
With this input, the one-hot encoded sequences are passed to a convolutional layer with 10 channels; each channel correspeonds to a different kernel or sliding window. Each of these sliding windows may learn different features to assist in the task of predicting expression strength. These 10 new representations of the input text are then pooled; the text is split into chunks of 2, the higher value is kept and the other discarded. This condenses the sequence making it shorter; which would enable future convolutional layers to learn spatial features. With these new representations, the numeric values of features of the text are flattened out into a list of values which is finally passed to linear layers to predict the final value.
The trained model is the most basic CNN; however it does a very good job at predicting expression values all things considered. The result is a model which can take any DNA sequence (under 313 base pairs) and predict its expression in yeast.
Predictions on the validation set before and after training demonstrating the ability of the CNN model to predict accurate expression values for the input promoters.
Developing a baseline Genetic Algorithm
This genetic algorithm is as simple as the CNN. It is more analogous to vegetative propagation or inbreeding plants. For example, bananas and other horticulural variaties are improved through mutation breeding without meiosis or crossing over.
In this genetic algorithm, we start with an initial promoter sequence and put it through our model to get its predicted strength. Then we decide that we have a target expression level we would like to reach. This will be our target for evolution.
First 200 individuals will be generated using our input sequence as the seed. Each of the 200 individuals will receive 1 random SNP mutation. These new sequences will be run through the CNN model to get an expression score. These scores will be compared to the target value to determine each of the new sequences’ (or indviduals) fitness. The top 50 individuals will get to undergo the next generation and perform mitosis; or clone themselves. so these 50 individuals will clone themselves and represent 150 of the 200 individuals in the next generations population. The remaining 50 will be randomly generated in the same fashion as the original 200. This process will be repeated for 50 generations; we could also set a stop condition (like when it reaches within 5% of the target value to top evolution and return the most fit sequence).
We can see how the genetic algorithm performs with varying numbers of mutations(just one parameter of the algorithm). We can see that the algorithm enables us to design promoter sequences effectively.
The genetic algorithm performs differently depending on the parameter of mutations per generation. The initial expression value was 0.8 and the target values was -0.5 (normalized values). The algorithm was able to find a solution to mutate/evolve the input promoter
Both the CNN and genetic algorithm are designed as baselines to be improved upon. Here are some ways each could be improved.
The CNN is currently in a basic state.
It’s a shallow network; we only have 1 convolution and pooling layer. Typically the advantage of convolutions emerges with many of these layers. So we could stack 3-5 of these to condense the sequence representation into a single value or just a handful. This would mean we pass a fraction of the values to the input layers at the end compared to our baseline model.
Additionally, we could add skip connections from the first convolutional layer to the final one; this perserves information present at the beginning which may be lost due poor filters or the pooling layers.
Kernel sizes; could be varied. This is important because it determines how many base pairs are considered at a given time, longer kernels may learn different features than shorter ones.
The genetic algorithm has many more improvements which can be made.
The fitness function ; doesn’t factor in the number of mutations from the original sequence; perhaps we only want to perform 3 SNP mutations. This has relevance in the real world due to political regulations which may constrain the number of ‘novel’ mutations which may be performed in an individual.
More mutations; could be added like inversions, deletions, additions which affect multiple base pairs at a time. This quickly adds to the diversity of the population.
Genetic Flow; could be simulated with multiple genetic algorithms running in parallel. By the nature of evolution they would like evolve in seperate manners. Individuals from each population (you can think of them as islands of individuals) could be swapped each generation.
Breeding; with other individuals present in the population to simulate crossing over between distinct branches of the population. This would enable the combination of multiple mutations of benefit must faster from individuals with high scores.
We can see how effective CNN’s and Genetic algorithms are a accomplishing the task of learning patterns in promoter sequences then optimizing them for a target expression value. These are two tools which, while effective, are not exactly cutting edge, however, assist in the understanding of how other machine learning and optimization models work.
The CNN was originally developed for images and has been subsequently used for text based tasks, especially in genetics. While the do consider spatial elements, they are not particularly good at preserving or utilizing them inside the model. Recurrent Neural Networks like the Long-Short Term Memory and Gated Recurrent Unit networks are more popular for text since they recognize patterns sequentially over one character or more at a time. Even more recent, is the application of transformers which are notable for being attention based; learning to focus on particular characters over the whole sequence.
Genetic algorithms have not been popular for a while, partly in due to the resurgence of neural networks which were only recently viable due to improvements of computational power, and key components improvements to components like stochastic gradient descent. A good example of this was the use of gradient ascent by Kotokpa and Smolke, who used this approach to develop new promoters with their CNN.
During the explorations of CNNs and genetic algorithms, I developed a new library for dealing with genetic data and deep learning models. All the code for this article has been deposited on github at https://www.github.com/cjgo/bionlp. If you are a student, you can work on implementing the aforementioned improvements. In the future, further development of this library will extend to various NLP models like transformers for working with DNA and Protein data.

domain: biology


Ex-tellers accused of lifting $117K-plus from vault
Published: December 11,2012
PEARL RIVER COUNTY — Two Pearl River County women have been charged with stealing more than $117,000 from a bank vault while they were tellers at a bank in Picayune.
The Sun Herald reports Vonetta Jordan was the head teller and Debra Mitchell was a teller when they took the money between April 2004 and February 2005. The bank at the time was known as Union Planters Bank. Regions Bank acquired the financial institution in June 2005.
A federal grand jury indicted Jordan, of Poplarville, on Nov. 27. She faces one count each of bank fraud and conspiracy to commit mail fraud, and 12 counts of making false entries.
Mitchell, of Carriere, was charged with bank fraud in a bill of information filed Nov. 30.
It was unclear whether the women have attorneys.
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Both men had Middle Eastern accents. The second guy kept saying "While on this federally recorded line are you willing to settle this situation"? I kept laughing at him saying that to attempt to scare me. I said I don't know what you're talking about and I know that this is a scam so I won't be paying anything, will be calling the Consumer Protection Bureau, and please take my number off of your call list at which time I was hung up on.
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The Photoshop CS6 Book is your ultimate guide to creating and perfecting stunning images and effects. Photoshop CS6 features revolutionary new features like 3D manipulation, motion graphics editing and erodible brushes, making this the most sophisticated version of Photoshop ever. This book helps you decode all of the outstanding features of CS6 with the help of step-by-step tutorials explaining these new features and all of the amazing capabilities of Photoshop's latest offering. Featuring: What's New in CS6 - Understand the latest features and tools of Photoshop CS6 Photo Editing - Use Photoshop to give your images the perfect finishing touch Digital Art - Learn to create infographics, master brushes and combine different media and effects to create something new Creative Projects - Get creative with your images by altering shapes, dimensions, warps and effects Please note: Digital versions of the bookazines do not include the covermount items or supplements that you would find on printed editions.
- Publisher: Future Publishing Ltd


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Lion City is: three room apartments located in the Austrian house suite on the 3rd floor five storey building.
Area of apartment is 99 square meters, has three rooms with separate entrances for 3, 8, 10 seats (beds have orthopedic mattresses), a shared bathroom, a common kitchen with a balcony.
Lion City Hostel is a new hostel opened in 2013, in Lviv. We will make your stay in the city is comfortable, bright and memorable. You can find us near the disco bar 'Metro'.
All rooms are equipped for your comfort and convenience, cable TV, cabinets; free Wi-Fi, internet access, iron. Bathroom has a hairdryer in common use, toilet, shower and sink.
Kitchen is equipped with everything necessary for a complete accommodation: kitchen utensils, electric kettle, fridge, stove, microwave, washing machine, two large tables for a comprehensive breakfast and lunch.
1st room: 3 Bed Family room area of 16 sq.m. with separate entrance, there are 3 single beds (size 2.00 to 0.90m), coffee table, large wardrobe with mirror, antique fireplace, free wi-fi, the window view goes out Konopnicka Str.
2nd room: 10 Bed Mixed room is 30 sq.m. with separate entrance, there are 5 two-stored beds with orthopedic mattresses measuring 2 m * 0.8 m, leisure sofa, flat screen TV, cabinets, fireplace, beautiful view from the window
3rd room: 8 Bed Mixed room is 22 sq.m. with separate entrance, there are 4 two-stored beds with orthopedic mattresses measuring 2 m * 0.8 m, cabinets, cable TV, hangers, fireplace, windows with view of the courtyard.
Cancellation Policy: 3 days before the arrival
Check In: 12.00 noon
Check Out: 11.00 am
Method of Payment upon the arrival: Cash only!
Breakfast is not included.
Taxes are not included.
14986 Hostels in Lviv (Ukraine)
starting from €
Our team is your service to help you with your booking issues or answer any related questions

domain: travel


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About Jupiter Island/Hobe Sound
Located at the southernmost end of Martin County, the city of Hobe Sound stretches along the Atlantic Ocean and deep-channeled Intracoastal Waterway, nestled between Port Salerno and the beautiful Jonathan Dickenson State Park. Hobe Sound’s unique personality comes from its split location on the mainland and on the northern section of Jupiter Island. Hobe Sounds has a relaxed, small town charm with beautiful beaches,...
Explore the Neighborhood
View Jupiter Island/Hobe Sound Listings


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I am 30 years old. Writer, smoker, hard drinker. Fan of Hunter S. Thompson and Ralph Steadman, both of whom are wrapped up in each other.
I bought a new camera recently, as well as a cell phone with it's own 3MP camera on board. I photograph what's around me. I know nothing of photography and it shows in my flawed eye. But I'm eager to learn, and will teach myself how to take a good photograph. Something I've yet to accomplish.
I also co-wrote and co-starred in a film called Necroville a few years ago. You can find it on store shelves, netflix.com or our website www.necroville.com.
Most popular photos
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Rufus: The Bat Who Loved Colours
Like all bats, Rufus sees life in black and white, until he chances upon an outdoor movie theatre and is awakened to the dazzling world of colours for the first time. Inspired and excited, Rufus paints his own wings and ventures out into the day, only to discover that his unusual appearance attracts unwelcome attention. Though he is initially rejected for his difference, Rufus soon learns to accept himself for who he is, thanks to a new friend.
First published in 1961, this classic story of difference and acceptance is as relevant today.
Illustrator: Tomi Ungerer
Binding: Casebound book with dust jacket


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A bankruptcy investigator lent credence to claims that Tribune Co.'s $8.2 billion buyout deal in 2007 left it too shaky to survive, throwing up another possible hurdle to the media company's efforts to exit bankruptcy protection after nearly 20 months.
The investigator, Kenneth Klee, said in a report Monday that it's "highly likely" that Tribune was "rendered insolvent and without adequate capital" as a result of the second half of the debt-reliant deal, which was led by real-estate investor Sam Zell. The company wasn't able to handle the debt load as the economy turned south, and Tribune filed for bankruptcy in December 2008.
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domain: economics


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On May 7 and May 14, 2021, winter ice still covered most of Lake Winnipeg and the smaller lakes nearby, and walleye were starting to show up on fishing hooks. By May 19, most of the ice was gone, temperatures hovered between 30° and 33° Celsius (86° to 91° Fahrenheit), and the parched landscape was blanketed with smoke and fire. It seemed like Canada’s Manitoba Province had jumped right from winter to summer.
On May 18, 2021, the Moderate Resolution Imaging Spectroradiometer (MODIS) on NASA’s Aqua satellite acquired this natural-color image of smoke billowing from several intense wildfires west and southwest of Lake Winnipeg. Strong westerly winds drove the plumes across Hudson Bay and the Canadian interior as far as Quebec. Other fires (not shown) burned to the west in Saskatchewan and to the east in Ontario. Researchers examining NOAA GOES satellite images detected hints of pyrocumulus “fire cloud” formation south of Lake Winnipeg.
Much of southern Manitoba and Saskatchewan had abnormally dry autumn, winter, and spring weather, so much of the region is now in severe to extreme drought. John Pomeroy, a water resources researcher at the University of Saskatchewan, told CBC News that soil moisture is about 40 percent of normal. The western United States and much of Mexico are enduring similar conditions.
Following a record-breaking spring heatwave and several days of gusty winds, Environment Canada declared fire danger to be “extremely high” in southern and central Manitoba. Provincial and regional governments have banned campfires in parks and closed many hiking and biking trails. Leaders of the Misipawstik Cree and Lake St. Martin First Nations encouraged residents to evacuate some areas due to encroaching fires, and several homes were destroyed. Parts of highways 5, 6, and 307 in Manitoba were shut down due to smoke.
NASA Earth Observatory image by Joshua Stevens, using MODIS data from NASA EOSDIS LANCE and GIBS/Worldview. Text by Michael Carlowicz.


New 2024 Hyundai Tucson SEL Models, Changes, Engine – Hyundai produced the Tucson SUV in the early 2000s as the first American vehicle to compete with the BMW 3 Series and Mercedes C-Class. Hyundai’s success with the 2024 Tucson led to its expansion to five Selection/E-Review trim sizes at some time. This resulted in the construction of the Tucson, a lowered SUV. As we began a new decade, Hyundai intended to alter the perception of its SUVs. They substituted the Tucson with a smaller SUV named the Hyundai Tucson.
A standard turbocharged inline 4-pipe engine produces 237 hp and 258 lb-ft of torque, comparable to the modern Audi A4, BMW 330i, and Mercedes C300. A superior two-turbo V6 with 335 horsepower and 400 pound-feet of torque is also available for the S4, M340i, and C43 (keeping that in mind, which has a small amount of sports flavoring). There is also a hotter 2024 Hyundai Tucson product or service, but it has its own review.
2024 Hyundai Tucson Redesign
Hyundai has introduced a fresh look with 2024 Hyundai Tucson. It lacks the velocity of its predecessor. As a result, it may be replaced by the Alpha 2 Chevy Camaro process, which might be utilized to manufacture new Tucson sedans. This Tucson turbocharged inline-numerous-conductor engine is more powerful than other standard engines.
However, the optional boost-turbo V6 provides more torque than the competition. The 2024 Hyundai Tucson has far more standard safety features and gizmos than its competitors. In addition, it may be used 12 times in 2024 as a guide for Hyundai’s suggested Unbelievably Holiday cruise vacation getaway hands-free vehicle management guide.
2024 Hyundai Tucson attempted to design the inside of its new luxury SUV to resemble those of its most recognizable German competitors, such as BMW and Mercedes-Benz. Tucson is an improvement on Tucson, yet it falls short of its intended purpose. The cabin is really annoying, yet it lacks ingenuity. This page contains several elements from other GM vehicles, as well as a significant number of elements that compare and reproduce BMW. People that utilize this component will typically be able to view and access the buttons for the infotainment and comfort functions.
You will learn about chair travel to the cabin for as many passengers as possible in the 2020 Hyundai Tucson SUV. Due to the fact that the vehicle is a “sportback,” there is limited headroom, and the access row is only large enough for well-known range seniors due to the back stop. Because there is some legroom, you can seat your closest friends in front of you at any time.As a general rule, the most advantageous characteristic of a car seat is its 12-way power-adjustability due to its two-way lumbar support and support.
There are two engine that may be purchased through the entire roof of the Tucson. For rear- or all-wheel drive, the standard 2.0-liter turbo-serial engine produces 237 horsepower and 258 lb-ft of torque.The Tucson can accelerate from 0 to 60 miles per hour in 6.6 seconds, which is slower than the BMW 3 Series and Audi A4’s 5.5 seconds. It takes 6.9 seconds with AWD. You should go for the 3.0-liter two-turbo V6 in the Remarkable Wonderful, which produces 335 horsepower and significantly more torque than the 3.0-liter V6 in the Remarkable Wonderful, which is already athletic. This engine is very powerful, and it propels the Tucson to 60 mph in 4.9 seconds.
A 3.0-liter boost-turbocharged V6 engine with 335 horsepower and 400 lb-ft of torque might be utilized if you want to acquire the most powerful vehicle. With a great deal of torque at the wheels, the V6 easily passes slower traffic, and when the accelerator is pressed, it emits an addictive roar as the speedometer rises rapidly. This is easily the best-sounding V6 Hyundai has ever produced, and it sounds far better than the two twin-turbo V6s found in the outgoing Tucson.
2024 Hyundai Tucson Release Date and Price
The 2024 Hyundai Tucson simple-to-use, high-end clip contains a variety of functions. However, the high-quality high-end offers many more features for the same price as the user-friendly high-end. Keeping this in mind, the sports footage does not correspond with the badge, which has several creative alterations. Therefore, we recommend that you go with High-quality High-quality, which boasts an abundance of modern safety systems, including lane departure warning, blind-spot monitoring, rear cross-traffic alert, and a powerful V6 engine. The wireless recharging of your tablet may be advantageous for those who are addicted to technology.


text: Kimo's is one of the landmarks on Front Street Lahaina. The food is fresh and well prepared. The menu has changed a little but in a good way. Always one of our must do stops while on Maui. The burger, ribs and other local favorites are worth the trip. eating lunch on the patio there are great ocean views. The staff is attentive and fun, the drinks are the best..


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The Or Bio Sparkling Extraordinary Oil contains a unique synergy of 5 unusual and precious organic oils combined with pearlescent natural pigments and an addictive natural scent.
Argan oil from Morocco, known for its exceptional skincare properties, is combined with two more unusual oils: Kendi oil from Indonesia and Inca Inchi oil from Amazonia, with regenerating properties. Buriti oil from Brazil helps nourish the skin and promotes a healthy skin glow while Pracaxi oil from Amazonia improves hair shine and strength.
Natural mineral pigments (Mica, Red iron oxide & white titanium) bring a light hint of elegancy.
Result: this 100% natural dry oil nourishes and beautifies the skin and hair while enveloping them in a soft, delicate scent. The light, fluid texture sinks in quickly, without leaving an oily film. Delicately scented, your skin is deeply nourished and instantly sublimated.
Natural and Organic Cosmeticcertified by ECOCERT Greenlife according to ECOCERT Standardavailable at http://cosmetics.ecocert.com99% of the total ingredients from natural origin44% of the total ingredients from Organic Farming
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