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The cryptocurrency industry is proliferating, and it’s important to stay up to date with the developments.
How To Make Money With Bitcoin
Want to Start Making Consistent Crypto Profit Every Day? Find Out More Here!
If you are new to the crypto space and are aiming to ramp up your knowledge of Ethereum, Bitcoin, and Blockchain, we have put together a list of the leading 5 best crypto courses for you! How To Make Money With Bitcoin
1. Blockchain A-Z: Learn How To Build Your First Blockchain
Blockchain A-Z is among the most extensive blockchain courses offered online. It is an exceptional tool for both novices and intermediate students and covers all topics, from Bitcoin to Solidity.
The course features over 20 hours of on-demand video, full lifetime gain access to, and a certificate of completion. Upon completing the course, you will end up being a Blockchain Expert in regards to knowledge, skills, and capabilities.
Blockchain A-Z utilizes Python programs language and will teach you how to construct your own Blockchain from scratch. The course also covers the use cases of blockchain technology throughout various markets and will allow you to produce clever agreements using Solidity on Ethereum.
The course focuses on the practical side of constructing a blockchain and how to build wise contracts using Ethereum. In addition, you will be able to create your own private Blockchain and release clever contracts on it for testing.
By the end of this crypto course, you will have an understanding of the following:
• What is a blockchain.
• What are decentralized applications.
• What are smart agreements.
• How to set up your own personal Blockchain.
• How to mine cryptocurrency.
• Blockchain terms.
2. Mastering Bitcoin by Andreas Antonopoulos
Mastering Bitcoin by Andreas Antonopoulos is a totally free book (PDF, EPUB, MOBI) about Bitcoin published in 2014.
The course describes the technical concepts behind Bitcoin and how they are implemented, and how to use the Bitcoin software application to develop brand-new deals and communicate with existing ones. Power To Pivot Workshop.
This course isn’t an extensive reference work on Bitcoin. Instead, it’s for non-technical readers who wish to comprehend the core concepts of this brand-new innovation and its execution but do not want to become developers or compile source code.
Mastering Bitcoin offers a detailed insight into Bitcoin for non-technical readers and technical information for more advanced readers. The course remains in 3 main parts:
• The very first part covers the technical foundations of bitcoin, its protocols, and its applications. It explains the bitcoin procedure in detail and discusses how cryptography is used to protect transactions using sophisticated mathematics and theory.
• The second part explains various applications for bitcoin users, such as wallets and mining. It likewise discusses how to use bitcoin to construct new financial applications. It covers subjects such as security steps, establishing tools for designers, and applications for non-technical users such as merchants and exchanges.
• The 3rd part explains the future of bitcoin, including possible scenarios for regulators, financial institutions, and technology service providers such as Google or Amazon.
3. Blockchain and Bitcoin Fundamentals by George Levy
Blockchain and Bitcoin Fundamentals by George Levy is one of the best cryptocurrency courses available (4.7/ 5 rating on Udemy). It has more than 13,000 trainees and focuses onBitcoin and Blockchaininnovation basics.
The course offers a standard understanding of blockchain innovation and its application. This course is taught by a knowledgeable bitcoin expert involved in blockchain technology for almost a decade. The best part of this course is that it is developed for beginners, so you can comprehend everything, even if you are brand-new to the world of cryptocurrency.
The instructor covers every subject from its meaning, how it works, applications to utilize cases, and future scope. While discovering the concepts, you will also see some hands-on examples from real-world use cases. The course needs no anticipation and covers all the fundamentals that you require to understand about bitcoin and blockchain innovation.
The Blockchain and Bitcoin Fundamentals course is divided into eight sections. These cover whatever from a quick history of Bitcoin to technical details on how blockchains work. You’ll discover hash functions, elliptic curve cryptography, and the double-spending issue, all without any mathematics or computer technology needed. The course likewise consists of 2 programming workouts to put your newly found knowledge into practice.
• The history of cash.
• How Bitcoin was produced.
• What Bitcoin is.
• How Bitcoin works.
• Why Bitcoin has value.
• How to buy and sell Bitcoin.
• What “cryptocurrency” implies.
• What Blockchain is and how it works.
• What “mining” indicates and how it works.
• How to buy and sell other cryptocurrencies (altcoins) like Ethereum and Ripple (XRP).
And far more!
You’ll also discover to avoid common mistakes when purchasing or selling cryptocurrency. And you’ll get answers to all your concerns with real-world examples that make learning fun.
4. How To Make Money With Bitcoin
The Cryptocurrency Certification Consortium (C4) is a non-profit organization with the mission to develop a globally-recognized requirement of proficiency for blockchain experts. The accreditation program has actually been developed by experienced industry specialists who have actually played crucial functions in leading jobs within the space.
The C4 task is intended to be an open-source effort with contributions from its members and other developers operating in the area of cryptocurrencies, decentralized applications, and distributed computing networks.
The C4 Blockchain Professional Certification Program is developed to provide individuals with a standardized technique of evaluating their proficiency level within the field of cryptocurrencies. People must pass several exams to earn an accreditation credential that recognizes this expertise. The certification exam content will be developed based upon the cumulative feedback from C4’s subscription body through a study of its members and other developers working in the area.
The Certifications, which are based on the book Blockchain Basics (O’Reilly) by Daniel Drescher, include 4 courses:
• Blockchain Basics.
• Hyperledger Fabric.
Each course is developed for conclusion in 4-6 weeks by spending roughly 1-2 hours per week on coursework. A great prospect should have some background in IT however no anticipation of cryptocurrency or blockchain innovation. Nevertheless, the program is designed so that those without any prior IT experience can effectively become certified. Each course has a last test that consists of a minimum of 50 multiple-choice concerns and a task in which you will utilize the innovation to build an easy app.
5. Mastering Ethereum: Building Smart Contracts and DApps by Andreas Antonopoulos
This crypto course will teach you how to develop clever agreements and decentralized applications (DApps) on the Ethereum blockchain. You will establish your own cryptographically secure digital identity and explore how to utilize it to communicate with DApps. You will likewise learn more about Ethereum’s virtual maker, the most effective computing engine out there, and develop a decentralized application that operates on this machine.
Mastering Ethereum covers:
• How Ethereum works.
• Ethereum’s function in cryptography.
• How cryptocurrency works.
• Technical information of transactions and smart contracts.
• The function of mining in a Blockchain platform like Ethereum.
• Proof-of-work algorithm (PoW).
• Future developments in Ethereum such as Casper, Sharding, and Plasma.
This course is ideal for anyone looking to construct DApps on Ethereum. Whether you are a business designer, blockchain lover, or experienced developer, this course will use you an extensive guide to the world of Ethereum advancement. How To Make Money With Bitcoin


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Phone: (814) 641-3358
Jonathan is an ‘08 graduate of Juniata and has been working as a member of the staff in the Glaeser Center for Counseling Services since 2015. After beginning a career as a geologist, Jonathan pursued an M.A. from Immaculata University in Counseling Psychology and spent several years working in residential treatment for individuals with addiction and co-occurring disorders. Jonathan practices from a person-centered foundation with primary theoretical orientation in Cognitive Behavioral Therapy (CBT), Dialectical Behavioral Therapy (DBT), or Motivational Interviewing. Some of his personal interests are exploring, carpentry, woodworking, and the themes of perseverance, strength, and compassion in all forms of story-telling. Jonathan lives in Huntingdon with Kim and their children, Evelyn and Elijah, two cats, two birds, and a snake.

domain: psychology


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Craig Muskaserves as the Director of Investments for Threshold Group’s Foundation Services. Craig joined Threshold Group in 2009 after serving as Managing Director at IW Financial, a Portland, Oregon-based investment research and consulting firm. He researched international equities, merger and acquisition opportunities and other asset classes as part of the investment team at both Credit Suisse HOLT and Nuveen Investments.
We’re here to equip you with the tools you need to make a meaningful impact.Contact NCFP for support!
Keep up with trends in family philanthropy.Subscribe to our newsletter!
Gain access to personalized support and a network of peers.Join our community!

domain: economics


COMPANY NEWS; Chris-Craft Acts On Time Warner
By The Associated Press
Published: September 16, 1989
Chris-Craft Industries said it was appealing a Delaware judge's ruling that the company, a major shareholder in Warner Communications Inc., has no power to veto the merger between Warner and Time Inc.
The ruling last week by Chancery Court Judge William Allen said the terms of a class of Warner preferred stock held by a Chris-Craft subsidiary, BHC Inc., did not entitle Chris-Craft to vote on the merger.
A Chris-Craft attorney, Charles S. Crompton Jr., said the company was asking the Delaware Supreme Court to overturn the ruling on the ground that Judge Allen had inaccurately interpreted the terms of the preferred stock, which Chris-Craft maintains is convertible to voting common shares.


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About the film
new york city san francisco, california restaurant owner widower childhood friends woman director reconnect rekindled romance childhood sweethearts
Always Be My Maybe
Release Date: May 31, 2019
|Slogan||:||«There's Always Been Something Between Them.»|
|Producers||:||Nathan Kahane, Randall Park, Ali Wong, Rich Delia, Brendan Ferguson, Nahnatchka Khan, Michael Golamco, Erin Westerman|
|Writers||:||Randall Park, Ali Wong, Michael Golamco|
Reunited after 15 years, famous chef Sasha and hometown musician Marcus feel the old sparks of attraction but struggle to adapt to each other's worlds.

domain: artistic


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Bog Walk Man Charged For Allegedly Stabbing Neighbour In AbdomenDecember 23, 2022
The Jamaica Constabulary Force (JCF) said an unemployed resident of High Top at West Prospect in Bog Walk, St. Catherine, has been charged with Wounding With Intent following an incident in his community on Thursday, December 22.
He is 62-year-old Cleveland Williams, otherwise called ‘Morris’.
The JCF said: “Reports from the Bog Walk Police are that Williams and a neighbour had a dispute about a goat, during which Williams used a knife to stab the man in his abdomen. The man was taken to hospital where he was treated.”
A report was made to the police and Williams subsequently was arrested and charged after a question and answer session.
He is scheduled to appear in the St. Catherine Parish Court on Friday, December 30.
WE also do obituaries, advertisement, and special coverage of funerals, birthday parties, weddings, and other milestones. Call or WhatsApp us at 876-305-4574 or emaail us at firstname.lastname@example.org.

domain: law


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(As written by Jim Lammers for Animation Magazine, November 2011)
©2011 All rights reserved.
Building a Multi-Dimensional Spy’s World
Mixing traditional animation with 3D-generated backgrounds allows for cinematic directing in FX’s animated hit series Archer.
Super spy Sterling Archer lives in a timeless netherworld that is part 1960s-style cold war and part pop art intermixed with modern trappings like cell phones, internet, and video players. Archer’s world also appears to be shot like a movie, with unique camera angles for each shot to help move the story forward. This is possible due to Atlanta production house Floyd County’s choice to use 3D environments for the show animation backgrounds. Rather than use traditional cel animation’s flat panoramas for pannable backgrounds, or completely switch to a purely 3D approach, the Archer animation process has taken a unique hybrid approach that blends 2D characters with 3D environments. Now beginning its third season, the show has been a breakout comedy hit for Fox’s cable network FX.
The show’s art style uses a realistic representation of human shapes and movements, eschewing squash and stretch and all other unphysical exaggerations, so the more literal computer animated backgrounds jibe well with the characters. Art director Jeff Fastner explains: “It was always Adam’s (Reed, Archer creator/writer) intention for the show to have these characters based in a more realistic world. It helps to define and push the reality of the series.” The Archer animation process uses Adobe Illustrator and After Effects, starting from photography of costumed models to create the show’s characters.
Photographed Animation Backgrounds on Frisky Dingo
Having developed techniques for both characters and backgrounds during production of the cult hit Frisky Dingo (which aired from 2006 to 2008 on Adult Swim), art director Neal Holman knew that the environments for this more complex show would present a challenge. “For Frisky we actually scouted Atlanta locations and secured permissions for photography/broadcast on many of the animation backgrounds. We would then process the photography for a painted look.” With Frisky Dingo set in the present day and scripts that occurred in ordinary settings, this approach was effective for the 11-minute episodes.
However, when Archer’s pilot moved forward in 2008, Holman expected to switch to a traditional painted background approach since there were so many sets of such indeterminate date, and the shows 23 minute length meant many more sets. When this got off to a slow start, Holman used his own 3D talents to prove a point: “I had done several backgrounds in 3-D for Frisky Dingo, so I knew that process of painting over the renders was one that could work. I built Malory’s office over the weekend, shot a test render to (Backgrounds Director) Eric Sims and by that Monday, we had a process we were all in love with.”
3D Animation Backgrounds in Production
The 3D environments take more time to develop than a simple painting or other 2D animation backgrounds, but once produced they allow for very low cost for any additional angles, variations, and color schemes. Finished sets can be saved so that it is also inexpensive for a future episode to return to any previously built set, even when new angles or paint schemes are required.
As the show moved from pilot to production it was clear that there would be a lot of 3D work to do. Floyd County surveyed its options and ultimately awarded the 3D production contract to Kansas City-area animation house Trinity Animation. Trinity was able to meet the demands of fast-turn, low-cost, and high-quality, starting with its first test set — the Hitleresque KGB office of Nikolai Jakov. Trinity’s diverse experience of architecture, product simulations, and technical presentations helped with the unique requirements of the show as well as its fluctuating labor demands.
Complex and highly detailed worlds have the potential to compete with the characters, especially when camera techniques like depth-of-field (distance blur) are not generally used. The paint process allows the hybrid techniques to blend visually. Neal Holman relates that “the thick, black outlines of our characters keep them from being overwhelmed by the detailed animation backgrounds. Our painting process has evolved from the pilot in a lot of subtle ways to keep them sharp without taking attention from the characters.”
Creative freedom with Computer Generated Animation Backgrounds
Going 3D with the environments makes for new challenges but gives new freedom for creativity. Movies are usually made with a single camera, repositioned shot by shot for the best ways to draw in the viewer and tell the story. The rapid pace of producing for television mean that live action shows are often shot in a near-live approach where a director picks the best angle of three mobile cameras as the scene unfolds in real time. The much slower methods of making an animated series make it even more risky to include a large number of unique and original computer animated backgrounds but Holman and Fastner have found it liberating. Holman explains, “Once an environment is built, I can move my camera anywhere I want, with any lens I want. Once rendered, a finished paint of that background can be done in three hours. For a recent episode that was in an entirely new environment, we had 143 different shot setups. We could not have done that using the traditional process on our current schedule.”
Jim Lammers is President of Trinity Animation, an Overland Park-based firm that has provided animation and graphics to clients worldwide since 1994. Lammers holds a bachelor of Science degree in Electrical Engineering from the University of Missouri. Trinity Animation has created animation backgrounds for Archer, Unsupervised and Chozen. He is also the author of several books on animation for Pearson Publishing.


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This DIY Pigeon Hole Display For Your Ornaments is a great way to build cheer this year. See instructions here. Put your cheer on display with this unique DIY Pigeon Hole Display For Your Ornaments. The full how-to is just a click a way.
Top 20 Pallet Christmas Tree Designs To Pursue
Most Creative Christmas Decorations - Crafty Morning
Reclaimed Wood Christmas Tree Ideas - 25 ways to create Christmas trees using reclaimed, pallet and salvaged wood and trim - Design Rulz
Christmas tree made out of a pallet
Pallet to Christmas Tree DIY Outdoor holiday decorations


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Today marks the 25th National Women’s Day Off, where women all over the country are encouraged to leave their jobs at 14:25, in order to draw attention to the gender inequality that exists in the workplace still.
Amazing how they’re actively doing something to show that there is still inequality, yet despite their efforts it still exists.
Tonight I was exposed to so much more than I thought I would be. I love documentaries, they always remind me of how little I am in this big, big world. I HIGHLY encourage anyone who has ever complained about anything to watch this, it really puts things to perspective. We have choices, we have a roof over our heads, we have access to internet, we are able to read, and can access drinking water.

domain: sociology


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Vito Sperduto had a problem. Mr. Sperduto, an investment banker at Wit Capital, needed to figure out how much Staples.com, the Web site of Staples, was worth. Staples, the giant office supply company, had sold part of Staples.com to Staples insiders and venture capitalists in 1999, the first step in a plan to spin off the Web business to the public.
After the Internet stock crash ended hopes of a successful offering, Staples decided on March 8 to buy back those shares for $7 each, more than twice what the insiders had paid.
Staples.com hired Mr. Sperduto and Wit to justify the buyout price, which valued the company at almost $900 million. It was a tough job, considering that Staples.com lost more than $140 million last year.
Newly disclosed e-mail messages provide a rare glimpse of the Wit investment bankers grasping for a way to justify the price, as they ultimately did.Continue reading the main story
From the start, Mr. Sperduto was concerned about the $7-a-share price, the messages show.
''Just got off the call with the Staples.com CFO the information is very light the projections are very aggressive,'' Mr. Sperduto said in a message to Mack Rossoff, the managing director of Wit's mergers and acquisitions department, at 11:20 a.m. on March 12.
Several hours later, Mr. Sperduto warned in another e-mail message to Mr. Rossoff that ''it is going to be hard to get to the $7 per share level.''
''The only way I can get close is to value the staples.com biz based on the amount of contribution to revenue of the combined company.''
Since Staples.com would account for an estimated 8 percent of Staples overall sales in 2001, it could be valued at 8 percent of the company, or about $5 a share. Institutional investors criticize that method, saying the Web site mainly cannibalizes sales from Staples stores and catalogs.
Mr. Rossoff responded a few minutes later. ''I don't think Staples trades on a revenue multiple,'' he said.
Mr. Sperduto then offered an alternative, based on Staples.com's projected earnings in 2005. If Staples.com made $107 million that year, more than twice the profit last year of eBay, he could justify $7 a share, he wrote.
''Right now I think the projections are too aggressive,'' he concluded.
The e-mail messages were provided to The Times by Jay W. Eisenhofer, who is suing Staples over the matter on behalf of the Teachers' Retirement System of Louisiana.
Mr. Sperduto changed his mind quickly. By March 15, he and Wit had concluded that $7 a share was indeed a fair price for Staples.com, based on its estimated revenue for 2001 and 2002 and potential cash flow from 2001 to 2005.
Wit was paid $150,000 by Staples.com for the banking opinion and has previously performed investment banking work for Staples.
Mr. Sperduto said last week that he and Wit stood by their opinion. The e-mail messages were ''completely taken out of context,'' he said. ''Those are early impressions in the process before we had the chance to complete our due diligence.''
John Mahoney, the chief financial officer of Staples, said that although the deal valued Staples.com at $900 million, Staples had to pay the insiders and venture capitalists only $50 million for the shares it did not own. ''At the end of the day, their firm concluded that this is a fair price, and I would think their conclusion is relevant,'' Mr. Mahoney said.
But disclosure of the e-mail messages is sure to further inflame institutional investors, who have complained that Staples executives are using the parent company's assets to bail themselves out of a failed Internet investment.
The depth of big investors' dismay is evident in an e-mail message to three top Staples executives from Robert Urquhart, a portfolio manager at Oppenheimer Capital, which owned 2.9 million Staples shares as of the end of the year, according to Securities and Exchange Commission filings.
''In my 20 years in working in this business,'' Mr. Urquhart wrote, ''I can identify maybe half a dozen other transactions where management and shareholder interests have so clearly diverged. In every case, the company was never able to reclaim its investor base. Investors have long memories and numerous easier options.
''This transaction will make it virtually impossible for us, in good conscience, to be shareholders in your company.''
Mr. Urquhart declined to comment on his e-mail message, which was also disclosed as part of the Louisiana teachers' lawsuit.
Mr. Mahoney said, ''We talk to investors all the time, and there's give and take.'' He said investors would have the chance to vote on the purchase proposal at the company's annual meeting in June.
Ted White, director of corporate governance for Calpers, the $152 billion California public employee pension fund, said Staples should drop its plan to buy Staples.com.
''It appears to be simply a failed investment in a dot-com,'' he said, ''and the conflicts of interest seem to be quite paramount here. The fact that the company came up with the number and then went to the bankers, I don't think that was the right thing to do.''
If the company insists on going ahead with the deal, Mr. White said, ''it's a huge credibility issue.''
Correction: May 23, 2001, Wednesday A headline in Business Day yesterday with the Market Place column, about a decision by Staples to buy back shares of Staples.com, referred to the online business incorrectly. While a planned initial public offering for Staples.com was shelved, it is not ''a failed dot-com.''Continue reading the main story

domain: news


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Postmortemfarction in patients who die within a few hours of the onset of myocardial ischemia continues to be difficult for the pathologist. Morphologic changes are not detectable with ordinary histologic strains in the first 6 hours of infarction, and the pathologic changes are somewhat equivocal until the inflammatory infiltrate appears in the infarction of about 12 hours' duration.1 In clinically suspected cases of early infarction, the pathologist is frustrated not only by this difficulty but also by the infrequent finding of coronary artery thrombosis. The cause of death is often listed as arteriosclerotic heart disease, and it is consequently assumed that the patient has died of a "heart attack," which becomes a "diagnosis of exclusion" in such cases. It is desirable, therefore, to find a more sensitive method for detecting early myocardial infarction.
Measuring elevation of serum enzymes released from damaged tissue is valuable in the clinical diagnosis and
HISTOCHEMISTRY AND EARLY MYOCARDIAL INFARCTION. JAMA. 1966;197(3):216. doi:10.1001/jama.1966.03110030110040

domain: medical


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It sounds like a Smiths song, but it is an actual place, and it’s wonderful: the cafe in the cemetery. A Viennese Kaffeehaus called Cafe Strauss has recently opened in a former funeral parlor just inside the gates of the Friedrichswerderscher Kirchhof on Bergmannstrasse. The outdoor seating on a sheltered patio overlooks well-tended 19th- and 20th-century graves; the indoor space is airy and spare with soaring arches. Just a few blocks from the noise of Mehringdamm and Gneisenaustrasse, Cafe Strauss is startlingly tranquil, the kind of quiet that makes you realize how loud the rest of the neighborhood is. It’s also cool on a hot day – like the day before yesterday, when I ordered the delightful iced coffee pictured above. A real iced coffee (no ice cream!), with a twist of lovely presentation. I also had a bowl of red currants and gooseberries with yogurt; tart berries featured prominently in the promising-looking selection of cakes as well. However peaceful it is to sit outside there in summer, I’m sure it will fit its surroundings especially splendidly in cold weather: the sight of chessboards stacked on the piano at the back of the cafe promised so much autumnal coziness it almost made me look forward to the end of summer.
The name Cafe Strauss is a triple-entendre: the Viennese waltz composers, the German word for ostrich, and the name of the couple who own the place, Martin and Olga Strauss. In a fascinating interview with the Tagesspiegel a few months ago, the Strausses said that the space they converted into the cafe first began to be used as a funeral parlor in the 19th century. At that time, bodies had to be laid out for three days out of fear of accidentally burying someone alive. Bells were tied to them in case they woke up; what is now the toilet of the cafe was the room where a watchman sat listening for the bells. I’d say that even by Berlin standards, “cafe toilet in the place a man used to sit waiting for Scheintoten to ring bells” is a pretty unusual repurposed space.
And how perfect is it to put a *Viennese* cafe inside a cemetery? I mean, Vienna is the city of the schöne Leiche, home to the world’s only funeral museum, a place whose love of baroque melancholy carries on into the 21st century. As recently as 2008, when longtime mayor Helmut Zilk died, four horses pulled his coffin to the Zentralfriedhof in a hundred-year-old glass coach after a two-hour funeral at St. Stephen’s Cathedral featuring performances of Bruckner’s Mass in D minor and the Blue Danube Waltz by the Vienna Symphony Orchestra. There’s nowhere like Vienna for funereal flair.
I like the way the cafe is integrated into the graveyard. One of the main paths of the cemetery runs in front of its terrace. As I drank my iced coffee and read the newspaper, people walked past with flowers, on their way to visit loved ones’ graves. Cemeteries are oases of quiet and green, and it makes sense for the living to spend time in them – sense both in terms of using urban green spaces and as a counterweight to our culturally unhealthy lack of contact with death, the dying, and anything that reminds us of our own mortality. Martin Strauss told the Tagesspiegel he thinks the time he and his wife spend in the cemetery makes them “live more consciously because here we’re always reminded that life is finite.” The cafe’s small selection of items for sale subtly encourages cafegoers to spend some time among the dead: along with their own infused syrups, they sell red grave candles and a coffee table book about Berlin gravestones called Unter jedem Grabstein eine Weltgeschichte.
The Strausses also said the cafe gets a lot of animal visitors, most notably a fox who reclines on the roofs of mausoleums to watch funerals, who keeps away rabbits that would otherwise eat the flowers from the graves.
Cafe Strauss, Bergmannstr. 42, Kreuzberg. Open Tuesday-Sunday from 9am (closing time seasonal depending on cemetery hours, open until 8pm in summer).

domain: geography


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Ingesting contaminated water can lead to illness in both humans and animals. One pet owner experienced their dog becoming sick after playing at a dog park where the water was likely contaminated with waste, leading to the pet contracting giardia, an intestinal parasite that causes diarrhea, especially in young dogs. Intestinal parasites like Giardia and Canine Intestinal worms can spread through dog waste and infect humans as well. Additionally, bacteria found in dog feces can cause illness in both humans and dogs, leading to symptoms like diarrhea and stomach cramps, and even death in extreme cases.
It is important for people to properly dispose of waste to prevent the spread of diseases from dog waste. Even though infectious diseases from dog waste are rare in Finland compared to other countries, it is still crucial to collect and dispose of waste correctly to minimize the risk of infection transmission. Multi-resistant bacteria are more common in dogs in Finland, posing a risk for difficult-to-treat infections in both dogs and humans. Viral infections between dogs and humans are also possible but rare, with effective vaccination programs helping prevent the spread of viruses like canine parvovirus. Proper waste removal, especially of diarrheal waste, can significantly reduce the risk of disease transmission.
Investing in real estate can be a lucrative opportunity for individuals looking to grow their wealth. From digital real estate in the metaverse to traditional real estate properties, there are various ways to invest in the real estate market. Working with a real estate agent can help navigate the complexities of the market and find the best investment opportunities. Whether buying, selling or renting property, a real estate agent can provide valuable expertise to ensure a successful transaction. As the real estate market continues to evolve staying informed about investment opportunities and market trends is essential for success in the industry.
In summary, proper disposal of waste is crucial when it comes to preventing infectious diseases from spreading among pets and humans alike. Additionally, investing in real estate can be a lucrative opportunity if done right with help from professionals such as agents who have knowledge about different types of investments available on offer within this industry sector.
Furthermore researcher Thomas Grönthal emphasizes that when snow melts pathogens from waste can flow downstream and accumulate


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Heat the oil on a pan.
Peel and chop the onions and garlic finely and add to the pan.
Cook until soft
Add the paprika then stir in the passata and tomato purée
Bring to the boil then add the beans
Cook for ten to fifteen minutes.
Add salt and pepper to season

domain: food


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J Virol Methods 192:44C50. cellular immune reactions to multiple HCMV peptides. Collectively, this work provides a basis for future development of DB as an HCMV-based particle vaccine. IMPORTANCE Development of a vaccine to prevent congenital HCMV illness remains a high priority. Vaccination with human being cytomegalovirus-derived noninfectious particles, or dense body, may constitute a safe vaccination strategy that mimics natural infection. The standard approach for purification of disease particles has been to make use of a multiple-step, complex gradient that presents a potential barrier to production scale-up and commercialization. In the study explained here, we employed an approach that combines treatment with an antiviral terminase inhibitor and purification by a simplified process to produce a vaccine candidate providing broad antiviral humoral and cellular immunity like Rabbit polyclonal to CD47 a basis for future development. INTRODUCTION Human being cytomegalovirus (HCMV) is an important pathogen that remains a priority for vaccine development to prevent disease influencing immunocompromised individuals as well as populations at risk of transmitting congenital cytomegalovirus disease (1, 2). We while others have demonstrated that noninfectious thick body (DB) arrangements are favorable applicants for vaccination (3,C7). These arrangements reap the benefits of an adjuvant aftereffect of the particle and a proteins structure similar compared to that of virions and present PTC-209 a lower life expectancy risk because they absence viral DNA (vDNA) (3,C7). The neutralizing antibodies induced by vaccination are essential in stopping viral entrance into prone cell types. The neutralizing antibodies in serum from contaminated people focus on several HCMV envelope glycoproteins normally, including glycoprotein B (gB), gH/gL/move (gH trimer), gM/gN, and gH/gL/UL128-UL131A (gH pentamer) (8,C12). Clinical research support the electricity of the HCMV gB subunit vaccine with MF59 adjuvant, which decreased HCMV acquisition in adolescent young ladies, in females, and in solid body organ transplant sufferers (13,C15). The multiple glycoproteins provided on DB (5, 6) may improve on previous vaccine approaches using the gB subunit by itself. A course III viral fusogen, gB works in collaboration with gH/gL or the gH trimer during entrance into cultured fibroblasts, whereas the gH pentamer is essential for efficient entrance into epithelial and endothelial cells aswell as some dendritic cells (16,C21). Within a prior report, we demonstrated that vaccination using a DB planning induced neutralizing antibody in mice that was with the capacity of stopping infections of both cultured fibroblasts and epithelial cells (7). Furthermore with their glycoprotein structure, DB bring tegument proteins that creates relevant cellular immune system responses. Evaluation from the storage T cell area of contaminated normally, healthy individuals provides identified Compact disc4+ and Compact disc8+ T cell replies particular to 151 from the 213 HCMV open up reading structures (ORF) and uncovered that the replies to specific goals is highly adjustable among people (22, 23). In transplant sufferers, HCMV-specific PTC-209 cytotoxic Compact disc8+ T cells concentrating on tegument proteins had been effective in reducing HCMV disease and viremia (24, 25). PTC-209 The capability to induce both wide mobile immunity and powerful neutralizing antibodies could be essential for a highly effective HCMV vaccine. Previously, we set up that DB induce mobile replies to multiple protein (7). Purification of DB needs separation from the DB in the DNA-containing virions and DNA-free non-infectious contaminants (NIEPs) that are created during HCMV infections. Purification by ultracentrifugation uses sequential negative-viscosity, positive thickness gradients made out of potassium and glycerol tartrate (3, 26). Our prior evaluation of glycerol tartrate gradient sedimentation-purified DB (GT-DB) and purified, soluble gB with adjuvant MF59 highlighted advantages of DB (7). Right here we concentrate on alternatives to glycerol tartrate PTC-209 gradient sedimentation purification. We created a combined procedure whereby a viral terminase inhibitor is utilized during infection to lessen the creation of virions and demonstrate that tangential stream purification (TFF)-purified DB (TFF-DB).
TMAs are normal prostate tissue (= 9), Gleason 6C7 prostate cancer tissue (= 10), Gleason 8 prostate cancer tissue (= 8), and Gleason 9C10 prostate cancer tissue (= 18). levels of endogenous GAS6 were identified in putative cancer stem cells (CSCs, CD133+/CD44+) compared to non-CSCs (CD133C/CD44C) isolated from PCa/osteoblast cocultures and in DTCs isolated from the bone marrow 24 hours after intracardiac injection. Moreover, we found that endogenous GAS6 expression is associated with Mer receptor expression in growth arrested (G1) PCa cells, which correlates with the increase of the CSC populations. Importantly, we found that overexpression of GAS6 activates phosphorylation of Mer receptor signaling and subsequent induction of the CSC phenotype and setting. However, expression of GAS6 was detected in DTCs present in the bone marrow which had been shed from the PCa tumors (Figure ?(Figure1E).1E). Together, these findings suggest that the MI-773 (SAR405838) bone marrow microenvironment alters expression of GAS6 by PCa cells. Open in a separate window Figure 1 Bone marrow microenvironment activates endogenous GAS6 expression in PCa cells(A) MI-773 (SAR405838) GAS6 (green) expression in cytokeratin 18 (CK18, red) expressing cells (white arrows) in human prostate tissue microarrays (TMAs) as detected by immunofluorescence staining. Blue, DAPI nuclear stain. Bar = 50 m. MI-773 (SAR405838) TMAs are normal prostate tissue (= 9), Gleason 6C7 prostate cancer tissue (= 10), Gleason 8 prostate cancer tissue (= 8), and Gleason 9C10 prostate cancer tissue (= 18). (B) Quantification of Figure ?Figure1A.1A. Data represent as mean s.d. (Student’s PCa tumors in SCID mice and PCa cells in bone marrow from PCa tumors in SCID mice as detected by immunofluorescence staining. Blue, DAPI nuclear stain. Bars = 50 m. PCa CSCs (CD133+/CD44+) express high levels of GAS6 in the bone marrow microenvironment To explore whether different phenotypic populations of PCa cells express different levels of GAS6 in the bone marrow microenvironment, PCa cells were segregated based upon expression of CD133 and CD44 from cocultures with osteoblasts results, studies were performed to assess the same question. For these studies, injection of PCa cells into SCID mice was performed and 24 hours later the PCa cells present in the bone marrow were segregated based on CD133 and CD44 expression and DHRS12 evaluated for GAS6 mRNA expression (Figure ?(Figure2D).2D). In line with the results, higher levels of GAS6 expression were observed in the CD133+/CD44+ population compared with CD133C/CD44C cells recovered from the bone marrow (Figure 2E, 2F). Using immunofluorescence staining, we next examined GAS6 expression in PCa cells identified in human marrow coexpressing CD133 or CD44. Here GAS6 expression was positively correlated with both of the CD133 and CD44 markers (Figure ?(Figure2G).2G). Collectively, these data suggest that the bone marrow microenvironment plays a significant role in the regulation of GAS6 by PCa cells, and in particular by CD133 and CD44 expressing CSC populations. Open in a separate window Figure 2 Cancer stem cells express high level of GAS6 in PCa cells in bone marrow microenvironment(A) Experimental model of isolation of PCa CSC cells cocultures of PCa cells with osteoblasts (OB). (B, C) Expression of GAS6 mRNA in CD133C/CD44C or CD133+/CD44+ populations from the cocultures of PCa cells with osteoblasts as quantified by real-time PCR. Data are representative of mean with s.d. (Student’s injection of PCa cells in SCID mice as quantified by real-time PCR. Data are representative of mean with s.d. (Student’s (Figure ?(Figure2A).2A). We found significantly higher levels of Mer mRNA expression in CD133+/CD44+ populations compared with CD133C/CD44C. In addition, Mer mRNA expression was significantly more pronounced in CD133+/CD44+ populations isolated from cocultures of PCa cells with osteoblasts compared with CD133+/CD44+ cells cultured alone (Figure 3E, 3F). Finally, Mer expression was closely associated with GAS6 expression in PCa cells in the bone marrow from a PCa patient by immunofluorescence staining (Figure ?(Figure3G).3G). These data suggest that expression of GAS6 and Mer receptor is associated with the growth arrest of PCa cells, which also correlates with the numbers of CSC populations. Open in a separate window Figure 3 Growth arrested cells by the associating with GAS6 and Mer receptor correlate with CSC populations in PCa cells(A) Expression of the cell-cycle specific protein markers corresponding to.
Supplementary MaterialsSupplementary Information srep39250-s1. this approach works with any fused proteins. Right here we genetically fused the December205 mAb with two fragments (42-kDa and 19-kDa) produced from the ~200?kDa merozoite surface area protein 1 (MSP1), referred to as MSP119 and MSP142, respectively. The administration of two dosages of DEC-MSP142, however, not of DEC-MSP119 mAb, as well as an adjuvant to two mouse strains induced high anti-MSP119 antibody titres which were dependent on Compact disc4+ T cells elicited by peptides within the MSP133 series, indicating that the current presence of T cell epitopes in antigens geared to December205+ Indoramin D5 DCs boosts antibody responses. DCs are a significant bridge between adaptive and innate defense replies. They could feeling irritation and an infection, and present pathogen-derived epitopes to T cells1 efficiently. Once turned on, T cells generate cytokines and will help activate antibody making B cells. Furthermore, DCs can also activate B cells to mature and make great affinity antibodies2 directly. For their central function in Rabbit Polyclonal to Collagen I the induction of immunity, manipulation of DCs can be an interesting technique to induce adaptive immune system replies. Among these strategies, the usage of mAbs to focus on DCs continues to be examined with achievement in various versions3 straight,4,5,6,7. That is achieved by the usage of mAbs that target different DC surface receptors fused to antigens derived from pathogens, malignancy cells, etc.8. The C-type lectin DEC205 (CD205) has been used with success to induce both cellular and humoral immune reactions5,6. Despite its manifestation by additional cell types as B cells and epithelial cells9,10, the DEC205 manifestation in DCs is responsible for T cell activation when the antigen is definitely targeted through a cross DEC205 mAb11,12. The use of a DC maturation stimulus together with the cross DEC205 mAb induces long lasting T cell immunity that can even lead to safety in some mouse models of illness13,14. In addition, the induction of specific antibodies against the targeted antigen has also been observed3,5. In summary, there is considerable data in the literature showing that antigen focusing on to DCs through the DEC205 receptor elicits CD4+ and CD8+ T cell activation as well as antibody reactions when the cross mAb is given in the presence of a DC maturation stimulus such as CD40, polyriboinosinic: polyribocytidylic acid (poly (I:C)) or CpG oligodeoxynucleotides3,5,6,13,15. Among the many antigens delivered to the DEC205+ DC subset we can cite the model antigen ovalbumin13,16,17, the tumor antigens survivin18, HER2/neu19, NY-ESO-120 and melanoma TRP221, and Indoramin D5 different pathogen-derived antigens such as HIV gag6,7,15, LcrV22,23, and CSP5,24. In all cases, solid Compact disc4+ T cell replies had been attained against defined peptides or against peptides produced from overlapping peptide libraries previously. Compact disc8+ T cell activation was discovered when December205 mAb was fused to ovalbumin also, NY-ESO-1, TRP2, HIV gag, or CSP, particularly when the Compact disc8+ T cells had been re-stimulated and purified with one peptides5,6,7,13,21. Nevertheless, in some full cases, the activation of the cells had not been discovered18,23. Used together, these total results indicated that these antigens possessed antigenic epitopes acknowledged by the disease fighting capability. Although much continues to be published by using different proteins, the choice from the antigen is not explored fully. Would any antigen have the ability to induce solid T cell and antibody replies if geared to the December205+ DC subpopulation? To start out handling this relevant Indoramin D5 issue, we fused the December205 mAb with two fragments from the merozoite surface area proteins 1 (MSP1) produced from lifestyle routine and participates in parasite invasion25. It really is portrayed as an ~200?kDa precursor on the top of merozoite, and undergoes successive proteolytic cleavages generating a 42-kDa fragment (MSP142) that’s additional cleaved into two items: a soluble 33-kDa fragment (MSP133) that corresponds Indoramin D5 towards the N-terminal area of MSP142 and it is shed in the free merozoite surface area26, and a membrane-bound 19-kDa C-terminal fragment (MSP119), which may be the only MSP1 fragment carried using the invading merozoite in to the brand-new red bloodstream cell27. An infection with network marketing leads towards the induction of antibodies that bind generally towards the MSP119 proteins28,29,30 while MSP142 is definitely thought to consist of T cell epitopes31 that help B cells to produce anti-MSP119 antibodies32,33. Antibodies and CD4+ T cells directed to MSP1 were shown to be associated with safety against malaria in mice33,34,35 and humans36. To study the differences in terms of antibody induction and T cell activation in the context of DEC205+ DC focusing on, we delivered MSP119 or MSP142 proteins to this subset through Indoramin D5 two cross mAbs, DEC-MSP119 and DEC-MSP142. Analysis of the immune response induced by.
Supplementary MaterialsS1 Fig: Position of RAS proteins from and with isoforms from human beings. and PQ within the poor Muv phenotype of comes from Fig 1D. B Same data as with A but graphed as Average # of Vulvas. ***P = 0.0001 and **P = 0.005 compared to control as indicated.(TIF) pgen.1008838.s002.tif (202K) GUID:?013FC6F7-8833-4CD7-A1D6-E3148E8A7830 S3 Fig: Oxidation of C118 of LET-60ras inhibits the specification of vulval cell fates. A Quantification of invaginations in the Pn.pxx stage. B. Representative images. Asterisks show the invagination that may develop into the main vulva and arrowheads point to invaginations that may lead to the development of pseudovulvas.(TIF) pgen.1008838.s003.tif (1.1M) GUID:?6EB36657-C231-4B50-9476-1CDA8B2C18C8 S4 Fig: Picropodophyllin Western blot analysis of MPK-1 levels in WT and mutants. A, B Relative expression levels of total MPK-1. Ideals are shown like a portion of the percentage of the indicated proteins compared to wild-type worms. A Relative expression levels of total MPK-1a and MPK-1b relative to the loading control Tubulin. B Relative expression levels of total MPK-1a and MPK-1b relative to the loading control Histone. Mean and standard error of the mean (SEM) of 3 self-employed experiments are indicated in the graphs. No significant variations were recognized, illustrating the Picropodophyllin significant difference demonstrated in Fig 3A and 3B arise from variations in the levels of pMPK-1a not total MPK-1a. C Primary scans of traditional western blots. 1: wild-type N2; 2:mutants treated with NAC and PQ. A Relative appearance degrees of total MPK-1a to Histone H3. Mean and regular error from the mean (SEM) of 3 unbiased tests MOBK1B are indicated in the graphs. No significant distinctions were discovered illustrating which the significant difference proven in Fig 3C and 3D occur from distinctions in the degrees of pMPK-1a not really total MPK-1a. B Comparative appearance degrees of total Picropodophyllin pMPK-1b and MPK-1b to Histone H3. Mean and regular error from the mean (SEM) of 3 unbiased tests are Picropodophyllin indicated in the graph. Although PQ might have an effect on total MPK-1b and pMPK-1b amounts, because of the high amount of variability zero significant differences were present statistically. C Primary scans of traditional western blots. The scanned images were cropped to boost focus and clarity upon the precise proteins. Molecular fat markers are indicated.(TIF) pgen.1008838.s005.tif (1.6M) GUID:?A70FC518-1FCB-4DB6-8A99-17E858940CA2 S6 Fig: Traditional western blot analysis of LET-60 levels in WT and mutants. A Primary scans of traditional western blots for Fig 3E and 3F. A representative blot was proven in Fig 3E. Examples packed in lanes are the following: 1: wild-type N2; 2:+ PQ; 6:+ NAC. The scanned pictures were cropped to boost clarity and concentrate upon the precise proteins. Molecular fat markers are indicated.(TIF) pgen.1008838.s006.tif (1.3M) GUID:?DD64897E-8484-4C8E-8E2D-CE0F62C95D6C S7 Fig: mRNA expression degrees of in C118S and C11D mutants. Evaluation of expression degrees of gene in wild-type N2; and mutants. A MEMBER OF FAMILY expression degrees of total MPK-1a in accordance with the launching control histone H3. Beliefs are shown being a small percentage of the proportion from the indicated protein in comparison to wild-type worms. No significant distinctions were discovered, illustrating which the significant distinctions proven in Fig 3G and 3F occur from distinctions Picropodophyllin in the degrees of pMPK-1a not really total MPK-1a. B Comparative appearance degrees of total pMPK-1b and MPK-1b in accordance with the launching control histone H3. MPK-1b level was considerably reduced in and compared to wild-type. It also appeared to be decreased in and and reached statistical significance. Mean and standard error of the mean (SEM) of 3 self-employed experiments are indicated in the graphs. C Unique scans of western blots. 1: wild-type N2; 2:and humans. The redox-sensitive cysteine we have revised by CRISPR with this study (C18) is definitely defined in blue.(TIF) pgen.1008838.s009.tif (450K) GUID:?C41C5FF7-CE01-4D39-B3D0-7E7BA10A82AE S1 Table: Numerical ideals for Muv data shown in bar graphs in all numbers. (DOCX) pgen.1008838.s010.docx (42K) GUID:?10289B8E-2A31-4267-AE5B-ABEDE62515ED Data Availability StatementAll relevant data are within the manuscript and its Supporting Info files. Abstract Reactive oxygen varieties (ROS) are signalling molecules whose study in intact organisms has been hampered by their potential toxicity. This has prevented a full understanding of their part in organismal procedures such as advancement, maturing and disease. In and mutants, which boost mitochondrial ROS, inhibit the experience of Permit-60rasgf on vulval advancement. In contrast, the antioxidant reduction and NAC of vulva advancement being a model, we present that both mitochondrial and cytoplasmic ROS action over the worm homologue of RAS (Permit-60ras) through a redox-sensitive cysteine (C118). Nevertheless, as opposed to what is normally seen in cultured mammalian cells, that C118 are located by us can be oxidized by hydrogen peroxide, than by superoxide or nitric oxide rather, which its.
Sjogrens symptoms (SS) is a chronic autoimmune?disorder that triggers the irritation from the salivary and lacrimal glands, leading to dryness from the optical eye and mouth area. SS. Her medical diagnosis was predicated on a previous background of dried out eye, dried out mouth, joint discomfort, fatigue, and headaches. Furthermore, she had very high?actions of swelling?and a positive anti SS-A antibody. She?improved clinically with LDN therapy. The second case is definitely a 66-year-old female with recorded SS based on a history of dry eyes and dry mouth, joint pain, and?elevated anti-SSA and anti-SSB antibodies whose joint symptoms responded to treatment with LDN. strong class=”kwd-title” Keywords: sjogren syndrome, low dose naltrexone, joint pain, fatigue, autoimmune diseases Intro Low-dose naltrexone (LDN) is definitely a unique compound that has pain-relieving and anti-inflammatory properties. Limited studies have shown benefit in helping relieve the pain in individuals with fibromyalgia and improving disease activity in autoimmune conditions such as inflammatory bowel disease and multiple sclerosis. A case report published last year shown performance of LDN in a patient with Sjogren’s syndrome (SS) based on improvement of?their symptoms and inflammatory markers. Two additional instances of SS offered in this article shown similar effectiveness in medical symptoms and decreasing of?inflammatory markers. Case demonstration Case 1 BB is definitely a 66-year-old woman seen who carried a?analysis of SS. The analysis was based on a history of joint pain without TAS4464 hydrochloride synovitis or tenderness, dry eyes, and dry mouth. She experienced a positive antinuclear antibody (ANA) 1/160 inside a speckled pattern; positive anti-SS-A and SS-B at 418 and 180 U/mL respectively (normal less than 100 U/mL). Her rheumatoid element (RF) was 175 IU/mL (normal less than 10 IU/mL). Some 18 months prior to her first check out she had started plaquenil orally at 200 mg per day, with improvement in her joint symptoms.?On her initial evaluation, she experienced well and her erythrocyte sedimentation rate (ESR) was 12 mm/h and C-reactive protein (CRP)?was 0.16 mg/dL (normal less than 0.80 mg/dL). The patient reported that she was feeling well. About?eight years ago, she was concerned about the risk of retinal toxicity TAS4464 hydrochloride from plaquenil, so she elected to decrease the dose to 100 mg daily. About five years ago, she halted plaquenil completely as she experienced well. Within four weeks of discontinuing the drug she developed slight joint pain and an increase in her ESR to 29 mm/h. About three years ago, due to increasing?pain she resumed plaquenil?200 mg daily. However, her symptoms persisted and her sedimentation rates remained elevated between 27 and 46 mm/h. Over the past year, her symptoms had been and elevated connected with bilateral synovitis in two?metacarpophalangeal (MCP) and two proximal interphalangeal (PIP)?joint TAS4464 hydrochloride parts. In addition, there is an associated upsurge in her inflammatory markers using a CRP of 11.3 mg/L. Her sedimentation price at that correct period was 35 mm/h. She elected to try LDN after improvement using a one-week span of prednisone. A month after getting on TAS4464 hydrochloride 1 mg of LDN, she noted much less inflammation and discomfort. Her ESR transpired to 28 CRP and mm/h normalized at 5.7 mg/L. Four a few months ago, she have been increased by her LDN to 2 mg daily. She felt well without the joint pain clinically. Her test didn’t present any tactile hands tenderness or swelling. While her ESR continued to be high at 40 mm/h, her CRP was right down to 4.6 mg/L. By a month ago, she continued to be asymptomatic with a standard ESR of 23 mm/h and a standard CRP of 2.1 mg/L. In November of 2018 Case two CC is a 24-year-old feminine initial evaluated. She observed a five-year background of chronic popular body discomfort, fatigue, head aches, and human brain fog. Her prior lab showed a poor ANA, an elevated ESR of 40 mm/h, and an elevated CRP of 7.8 mg/L (normal significantly less than 5 mg/L). She stayed symptomatic, despite a gluten free of charge Rabbit Polyclonal to NT diet plan, TAS4464 hydrochloride physical therapy, and antidepressants. Her medicines included IV gamma globulin. Hydrocodone 10/325 5 tabs daily, flexeril?qhs, benadryl daily, metformin, topiramate, baby aspirin, and metoprolol. She observed dried out eye and dried out mouth. Her exam confirmed common myofascial pain in four quadrants without synovitis. Lab work showed a positive anti-SSA antibody 42 U/mL (normal less than 7 ELISA method). Her ANA was positive at 1/80 homogeneous and her additional.
For individuals presenting with limited metastatic disease burden, known as the oligometastatic state of disease, a more aggressive treatment approach targeting the new or progressive metastatic lesions might improve patient outcome, with no or only limited toxicity to be expected from the treatment. which the majority ongoing after 30 months . Furthermore, opposed to polymetastatic patients, oligometastatic patients have a more indolent disease course . As a consequence, a different treatment strategy, called metastasis-directed therapy (MDT), has been developed. The rationale behind MDT is that the eradication of a low number of metastatic sites will hamper further metastatic dissemination, as it has been clearly demonstrated that metastases are an important Mouse monoclonal to Galectin3. Galectin 3 is one of the more extensively studied members of this family and is a 30 kDa protein. Due to a Cterminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. Galectin 3 is normally distributed in epithelia of many organs, in various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is upregulated during inflammation, cell proliferation, cell differentiation and through transactivation by viral proteins. source of further metastatic spread . Reducing further metastatic spread by MDT might improve progression-free survival and overall survival . Another advantage is the postponing of systemic treatment and, consequently, the accompanying side effects and financial burden, while keeping the patients quality-of-life unchanged. MDT can be achieved both by metastasectomy and stereotactic body radiotherapy (SBRT) [10, AZD2014 biological activity 11]. Compared to conventional radiotherapy, SBRT is delivered as a large dose in a few fractions, resulting in a high biological effective dose. Dosage is conformal around the target with a rapid fall-off doses away from the target to minimise the effect on the surrounding tissue . For non-cranial metastases of the prostate and bladder, a total of three fractions of 10 Gy is prescribed, which can be reduced to three fractions of 9, 8 or 7 Gy if needed to respect the dose constraints to the surrounding organs of risk. For mRCC, a higher dosage of three times 14 Gy is attempted. An example of a radiation treatment planning are available in Shape 1. Open up in another AZD2014 biological activity window Shape 1. Exemplory case of a stereotactic body rays therapy (SBRT) thinking about a metastatic bone tissue lesion. The usage of MDT in dealing with oligometastatic RCC, bladder/urothelial tumor (UC), and prostate tumor (PCa) is getting interest and recognition. With this topical ointment review, we summarise the prevailing evidence of the procedure with MDT in these tumour types and present a synopsis of on-going tests that may modification the procedure paradigm for these individuals in the foreseeable future. Renal tumor RCC represents 3%C5% of adult malignancies and makes up about a lot more than 330,000 instances per year world-wide . AZD2014 biological activity Up to 20% of individuals have in advance metastatic disease and AZD2014 biological activity about 20%C40% of non-metastatic RCC individuals will ultimately develop metastasis, situated in the lung typically, liver, brain and bones [14, 15]. Regular administration for mRCC is dependant on ICI and/or targeted therapy . Close AZD2014 biological activity energetic monitoring with deferred systemic treatment could be proposed in case there is asymptomatic individuals with limited disease burden. Prior to the option of tyrosine kinase ICI and inhibitors, the prognosis of mRCC patients was poor with a median survival time ranging from 6 to 12 months, and even lower in case of brain metastases [17, 18]. However, currently a median OS up to 33 months has been reported . Patients developing metachronous mRCC perform better than those presenting with synchronous mRCC . While in the era of treatment with cytokines cytoreductive nephrectomy (CN) provided an OS benefit in the selected patients [17, 20], the role of CN needs to be re-established in the current era of immunotherapy and/or targeted therapy . Multiple retrospective studies [22, 23] pointed towards an OS benefit for CN; however, the randomised trial CARMENA  demonstrated non-inferior OS when sunitinib alone was compared to CN followed by sunitinib in patients presenting with intermediate or poor-risk disease. Good performance status and good/intermediate International Metastatic Renal Cell Carcinoma Database Consortium and the Memorial Sloan Kettering Cancer Center risk classification were predictive for OS benefit with CN, suggesting that this subgroup of patients.
Supplementary MaterialsSupplementary Information 41467_2019_14046_MOESM1_ESM. relationship between Arf1 T-cell and appearance infiltration and activation along with individual success in a variety of individual malignancies. Our outcomes reveal that Arf1-pathway knockdown not merely eliminates CSCs but also elicits a tumor-specific immune system response that changes dying CSCs right into a healing vaccine, resulting in durable benefits. order SP600125 in mice disrupts their lipid outcomes and fat burning capacity in lipid droplet deposition, which in turn causes metabolic tension further, including mitochondrial flaws and endoplasmic reticulum (ER) tension. This metabolic stress kills cells enriched with RFC37 CSCs through necrosis in mice selectively. The dying CSCs discharge damage-associated molecular patterns (DAMPs), which activate dendritic cells (DCs). The activated DCs enhance T-cell infiltration and activation to stimulate an anti-tumor immunity further. Our outcomes reveal that knockdown from the Arf1 pathway provides multimodal features, it not merely eliminates CSCs but also elicits a tumor-specific immune system response where dying CSCs are changed into a healing vaccine to attract and activate immune system cells for destroying the majority tumors and leading to durable efficiency of the procedure. Results Arf1-governed lipolysis selectively sustains CSCs in mice is among the most evolutionarily conserved genes between and mouse, with an amino acidity identification of 95.6% between the two varieties (Supplementary Fig.?1a). We generated conditional knockout (CKO) mice and are using them to investigate how the COPI/Arf1-mediated lipolysis order SP600125 pathway regulates stem-cell and CSC survival in these animals (Supplementary Fig.?1b). Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5, also known as Gpr49) is definitely a Wnt target gene, and an inducible Lgr5-Cre knock-in allele (Lgr5-EGFP-IRES-CreERT2) focuses on genes to stem cells of the small intestine, colon, and various other adult cells and order SP600125 cancers9. The Lgr5-CreERT2/Apcf/f (Lgr5/Apc) mouse is the best-known mouse model of CSCs10. Lgr5-CreERT2 encodes a tamoxifen-inducible Cre recombinase11 that is indicated in long-lived intestinal/colon stem cells, while adenomatous polyposis coli order SP600125 (Apc) is definitely a tumor suppressor. Knockdown of using Lgr5-CreERT2 results in colon stem cell tumors9. Like Lgr5-CreERT2, Axin2-CreER is also selectively indicated in intestinal stem cells, and we found that the knockdown of by Axin2-CreER could efficiently ablate stem cells12 (Fig.?1a). We further examined the result of ablation in eradicating CSCs in Lgr5-CreERT2/Arf1f/f/Apcf/f (Lgr5/Arf1/Apc) mice and discovered that knocking down Arf1 significantly decreased the stem cell tumor amount (Lgr5/Apc: 95.8??17.8; Lgr5/Arf1/Apc: 48.4??18.1) (Fig.?1b, c) and significantly extended the life expectancy from the Lgr5/Apc mice (Fig.?1d). Open up in another screen Fig. 1 knockdown decreases the CSC and expands the life expectancy of Lgr5/Apc mice.a LacZ-stained parts of intestine from an Axin2-CreER/Rosa26R or an Axin2-CreER/Rosa26R/Arf1f/f mouse that was treated with five intraperitoneal shots of tamoxifen to activate stem-cell-specific Cre and facilitate the increased loss of Arf1. b Lgr5/Apc or Lgr5/Apc/Arf1 mice had been treated with three continuing intraperitoneal shot of tamoxifen to activate the stem-cell-specific Cre and facilitate the increased loss of Apc and Arf1. gFP and -Catenin tag stem cells. c Intestinal tumor amount in the indicated genotypes (stem cells, however, not in differentiated cells, disrupts lipid fat burning capacity and causes lipid droplet order SP600125 stem and deposition cell necrosis5. Progenitors, such as for example hepatoblasts, talk about many properties with stem cells. Our results which the knockdown of in developing liver organ with Foxa3-Cre led to lipid droplet hepatoblast and deposition necrosis, as the knockdown of in differentiated liver organ cells (with Alb-Cre) acquired no effect, recommended that Arf1 selectively sustains stem progenitors or cells in both and mice. We further utilized the acetaminophen (APAP) to stimulate the liver organ damage in the Alb-Cre/Arf1f/f mice and control (Alb-Cre/Arf1f/+) mice. We.
Supplementary Materialscells-09-00657-s001. confirmed the power of M2 receptor to inhibit EGFR and Notch1 appearance, highlighting a molecular relationship between M2 receptor as well as the Notch-1/EGFR pathways also in GSCs. (APE), lowers cell success and proliferation in GBM cell lines and in major cell civilizations [16,17]. Lately, we also confirmed the fact that selective activation of M2 receptors by APE or dualsteric agonist N8-Iperoxo inhibits cell development in GSCs extracted from two different individual tumor biopsies (GB7 and GB8 cells) [18,19]. To be able to better understand the systems root the reduced cell success and proliferation, in today’s work we referred to the power of APE to in different ways modulate the cell routine development in GB7 and GB8 cells. Furthermore, the cross-interaction between M2 receptors and Notch1/EGFR pathways continues to be looked into also, demonstrating the fact that APE-induced reduced cell proliferation would depend in the impaired activity of the two signaling pathways. 2. Methods and Materials 2.1. Cell Civilizations The glioblastoma tumor stem cell lines FTY720 cell signaling (GSCs) GB7 and GB8 had been obtained from individual biopsies [5,20]. The cells had been cultured on the laminin-coated plastic material (1 g/mL, Sigma-Aldrich, St. Louis, MO, USA) or as neurospheres (in uncoated plastic material) and taken care of in Euromed-N moderate (EuroClone, Milan, Italy) supplemented with 1% streptomycin, 50 IU/mL penicillin, (Sigma-Aldrich, St. Louis, MO, USA), 1% glutamine (Sigma-Aldrich, St. Louis, MO, USA), 1% N2 health supplement (Invitrogen, Monza, Italy), 2% B27 (Invitrogen, Monza, Italy), 20 ng/mL EGF (Recombinant Individual Epidermal growth aspect, Peprotec, London, UK), and 20 ng/mL FGF (Recombinant Individual FGF-basic, Preprotech, London, UK). The cell civilizations were taken care of at 37 C within an atmosphere of 5% CO2/95% atmosphere. 2.2. Pharmacological Remedies M2 agonist arecaidine propargyl ester hydrobromide (APE) was utilized to selectively stimulate the M2 muscarinic receptor subtype. The power of the agonist to bind the M2 receptor subtype once was confirmed in GBM set up cell lines (U87 and U251) and in GSCs (GB7 and GB8 cells) by pharmacological binding tests and FTY720 cell signaling knockdown from the receptors by siRNA transfection pool [17,18]. Epidermal Development Aspect receptor (EGFR) tyrosine kinase inhibitor (TKI) N-(3-chlorophenyl)-6,7-dimethoxy-4-quinazolinamine), tyrphostin AG1478 (Sigma-Aldrich, St. Louis, MO, USA) was utilized at final focus of just one 1 M, to inhibit the EGFR pathway . 2.3. Immunocytochemistry GB7 cells had been plated onto 35-mm-diameter meals in complete moderate. After that, the FTY720 cell signaling cells had been rinsed with phosphate buffer saline (PBS) pH 7.4, fixed with 4% paraformaldehyde for 20 min in room temperatures (RT), washed in PBS, and permeabilized by treatment with blocking buffer (0.1% Triton X-100, 10% NGS in PBS) for 1 h at RT. The cells had been FTY720 cell signaling then incubated right away at +4 C with anti-Nestin (1:200, Abcam, Cambridge, UK), anti-CD133 LIMK2 (1:100, Miltenyi Biotec, Teterow, Germany), anti-REST (1:200, Abcam, Cambridge, UK) antibodies diluted in antibody incubation buffer (0.1% Triton X-100, 1% NGS, 1% BSA in PBS). The very next day, after three washes with PBS, the cells had been incubated for 1 h at RT using a goat anti-mouse-Alexa 594-conjugated (1:2000, Promega, Madison, WI, USA) or goat anti-rabbit-Alexa 488-conjugated (1:2000 Promega, Madison, WI, USA) supplementary antibodies diluted in incubation buffer. After cleaning in PBS, the cells had been finally installed with 30 L of Anti Fade Mounting Moderate with DAPI (Immunological Research, Rome, Italy). Harmful controls were attained by omitting the principal antibodies (data not really proven). 2.4. RNA Removal and RT-PCR Evaluation Total RNA was extracted through the use of Cultured Cell Total FTY720 cell signaling RNA Removal Mini Package (FMB, PA, USA) following manufacturers guidelines. RNA examples (2 g) had been slow transcribed for 60 min at 37 C with Random Primers (Promega, Madison, WI, USA) and M-MLV slow transcriptase (Promega, Madison, WI, USA). After that, PCR reagents, primers,.

domain: biology


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Here at Calluna we are always looking for new ways to highlight and honor some of the amazing creative partners we get to work with. We feel incredibly fortunate to work with some seriously talented vendors in the industry, and we want to show them off to you in our new Calluna Community Series! We will sit down with some of the fabulous vendors of Colorado and get to know about them, their business and what life is like behind the scenes. Stay tuned for more!
This week for Calluna Community we caught up with Laura of Laura Murray Photography!
Cheers! Xo, Calluna
Describe what you do! “I am a wedding and lifestyle photographer. I am based in Golden, Colorado, but I adore traveling for work all across our beautiful state, as well as across the country and globe. My photography business began in 2008 while planning my own wedding. At the time I was working in a complete opposite field– I have a degree in mathematics from the University of Colorado and my career path initially took me into tech consulting. However, I craved creativity and have always had an entrepreneurial spirt. I fell in love with photography while working with my own wedding photographer. I quickly made the switch and have never looked back! I absolutely love what I do — my job is my passion and I cannot imagine doing anything else for work. I get to work with the loveliest people on their happiest of days. Being a wedding photographer is my dream job.”
What is your idea of a dream project? “My dream project is working with clients who make their wedding a reflection of their unique selves. My favorite thing is to walk away from a wedding with the feeling that the couple created an event that was a true representation of their personalities and relationship. I also love working with couples who value emotion and are not afraid of expressing it. My favorite imagery is created when when couples do not hold back from expressing their love and joy.”
What has been your favorite wedding that you have worked, or attended? “Oh goodness, it is so hard to choose, as I have so many favorites. I feel like I have the BEST clients around. My clients are kind, gracious, and have incredible style. Each wedding is completely different, but always so enjoyable. However, traveling is always a highlight for me. I have enjoyed traveling to Paris, Santorini, New York, California, Montana, and Vermont to photograph weddings. And I never tire of traveling across the state to Colorado. It is exciting to be somewhere different each weekend and to draw inspiration from my environment when photographing.”
What does your morning routine look like? “My morning routine is a little different now that I have kids (I have 3 kids– a toddler and twin 7 month old babies). Because I often work on the weekend, I allocate some weekdays as family days. On those days I spend my morning getting in some snuggles with my children, eating breakfast together, and we often go outside to play. However, on work days I try to get into work mode right away. I wake up around 7 AM and once the kids are with our nanny, I start my work day. I have a studio space in my home and it is very convenient. It is a light-filled location where I will meet clients, do computer work, and also photograph studio sessions. On a typical day, I will first take a look at all my emails and respond immediately to anything that needs a quick reply. Communication is a keystone of my business so I like to be timely with emails. Then, I plan my day depending on what I have going on. My work days are often filled with editing, album design, blogging, creating social media content, reviewing/drafting timelines for upcoming weddings, and meeting with potential clients/current clients. I also like to carve out time to work ON my business instead of always IN my business. Working ON my business means setting strategic goals, improving who I am as a person and photographer, and planning out the steps required to meet my long-term goals.”
What are some of your favorite trends that you are seeing? “One of my favorite trends has to do with fashion, especially with the wedding dress. I think the wedding dress is the perfect way for a bride to express her personality. I have seen so many different styles of wedding gown — and all are so beautiful! Sometimes my brides choose something more modern with sleek lines and unique shapes, sometimes they choose something more traditional with intricate lace patterns or beading. My favorite trend is when a bride choose a gown that is a reflection of their personal style.”
What advice would you give to a younger you? “I would tell my younger self that you should dream big, have no regrets and that you are responsible for creating your own happiness. Dream big with your career and don’t settle for a 9-5 that doesn’t inspire you. Have no regrets by getting out of your comfort zone; travel the world and ask that cute stranger out on a date (which is how I met my husband!). The sooner you realize that YOU are responsible for your own happiness you can get on with creating a wonderful life and not blaming others for your apparent failures/shortcomings.”
What makes you excited to go to work in the morning? “My clients and the wedding vendor teams that I work with are what ignites the fire in me. As I said before, I love my clients. They are always such a joy to be around! But I also have such a strong community of vendor friends too. The wedding industry is warm and welcoming — many of the people I worked with at weddings have become dear friends. My answer to this question is that the PEOPLE I work with are what makes me excited to get to work!”
What is your favorite cocktail, or what are you drinking tonight? “My favorite cocktail is usually a glass of red wine. You really cannot go wrong with a good glass of red wine and it is also heart healthy (I am a health nut!). But if I am going to splurge, I’ll choose a chocolate martini! I can never get enough chocolate in my life! ;)”
Follow Laura Murray Photography on Instagram!


Roshan Mathew, Dibyendu Bhattacharya, and Nimisha Sajayan, who have all played important roles in this season, are the show's stars. Superstar mahesh babu recently discussed his thoughts on the show and his feelings after seeing Poacher. Mahesh Babu posted a poster of the Poacher series on his official instagram account on february 27. The caption read, "How can anyone do it... Don't their hands quiver?" I can't help but wonder things like these after seeing #PoacherOnPrime. A moving appeal for us to take action to save these gentle giants.
For those who are unaware, the show centres on a group of indian Forest service employees named Neel (Dibyendu Bhattacharya), Alan (Roshan Mathew), and Mala (Nimisha Sajayan), who risk their lives to solve a challenging case involving the poaching of helpless elephants.


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Thinking outside the box to build a square
Innovative financing fuels Quincy Center redevelopment
Cities and towns across the Commonwealth are being hit harder than ever as revenue and state aid decrease. Many municipalities have put on hold development projects that could revitalize public spaces critical to long-term growth and sustainable development. Consequently, municipalities need to start thinking more creatively and boldly about how to work with developers in public/private partnerships to develop projects that will generate revenue, create jobs and breathe new life into regions across the state. In this process, however, municipalities must be careful to negotiate protections to avoid negative financial impacts or the blight of stalled projects, such as the Filene’s site in downtown Boston.
The ambitious, large-scale redevelopment of Quincy Center that is now getting underway provides municipalities with a good example of moving a project forward by combining a mixture of innovative thinking, prudent protections, and community benefits for the city. For years, city leaders had been working to redevelop Quincy Center and to create a mixture of retail, office, hospitality and residential spaces. Street-Works Development LLC, a New York-based developer, approached Quincy with a novel way to finance a massive $1.3 billion project, one that had never been tried in Massachusetts. Our charge as the legal team for Mayor Thomas Koch was to provide in any agreement an unprecedented level of taxpayer protection and to ensure that revenues to the city always outpace the city’s costs. All or some components of the city’s approach might provide a helpful model to other municipalities.
In a nutshell, Street-Works, as the redeveloper, will purchase two city properties (a parking lot and a parking garage) and combine them with other privately acquired land. Street-Works will then acquire the necessary permits and use private financing to make significant infrastructure improvements, such as new roads, sidewalks, landscaping and garages needed for the overall project.
After the infrastructure improvements and the private development components are constructed, and after other important negotiated preconditions are met, the city of Quincy will issue municipal bonds to reimburse Street-Works for its costs in constructing the infrastructure improvements. The debt service on these bonds will be paid principally from Chapter 121A payments to be made to the city by Street-Works after the project has started to generate revenue from sales and rent, plus revenues from the new city-owned parking garages.
What makes this approach unique is that, instead of a city first taking land, building the necessary infrastructure, and then trying to find interested developers, Quincy is leveraging its power to issue bonds in order to assure the developer that, as long as it meets certain requirements, its investment in initially privately constructing the future public infrastructure will be covered.
The use of the 121A payments is another unique factor in this project. Chapter 121A was adopted by the Legislature in the 1960s to encourage development in urban areas. If a proposed redevelopment project satisfied certain urban renewal criteria, the owners would not have to pay standard real estate taxes based on the city’s assessment of the building. Instead, the owner would pay a lower excise tax to the state based upon a statutory formula, plus certain so-called Section 6A payments usually negotiated with the local municipality. The Prudential Center and the TD Bank Garden in Boston are examples of 121A-inspired projects.
What’s different about the Quincy Center project is that Street-Works has agreed to make 121A payments to Quincy that will be more rather than less than the amount of assessed real estate taxes when its project is completed. This approach may sound counter-intuitive, but this higher payment ensures that Quincy will have a robust source of revenue to finance the debt service. To keep this funding stream as strong as possible, the city placed in the agreement a provision that, after a period of time, the city could elect to switch back to assessed real estate taxes if such taxes would be higher than the 121A payments. In return, Street-Works (and its construction lender) will have the security of knowing that Quincy will use its bonding power to reimburse them for the infrastructure improvements they made to facilitate the private project.
To make sure that Quincy is protected in this venture, the city’s counsel worked with the entire city team to create a set of protections and benefits that were written into the land disposition agreement. Other cities may wish to consider at least some of these requirements or principles when negotiating their own public-private partnership arrangements.
Among the principles and requirements are:
- The city’s general fund is never used, and the redeveloper’s money is always in the deal ahead of the city’s money.
- The city can review the redeveloper’s plans, apply design standards, and review the redeveloper’s bidding process.
- There is a bond cap established for each phase, after which the redeveloper pays for all cost overruns, and there are strict limitations on the types of costs that the redeveloper can include in its reimbursement budget.
- Pre-conditions are established to assure timely commencement and completion of construction. If the redeveloper fails to meet certain permit milestone dates, the project is not commenced in a timely manner, is suspended, or is not completed by certain dates, title can revert to the city and escalating financial penalties are imposed.
- The redeveloper must show sufficient equity and debt financing before the city is obligated to close the deal.
- The redeveloper must work with the city to secure necessary federal and state funding; the city has termination rights if this funding does not come through by certain dates.
- The establishment at closing of a community benefits cash account to be used for other public purposes in the city.
- Affordable housing linkage payments for every residential housing unit within the project.
- Agreements to use a high percentage of union labor, with preferences for hiring local residents for construction and permanent jobs, as well as purchasing local goods and services.
- The redeveloper must acquire all private lands needed for the project with no use of the city’s eminent domain powers.
Robert Fishman is a real estate partner at Nutter McClennen & Fish, LLP, where he chairs the firm’s Land Use and Development Practice Group. He serves as a special counsel to the city of Quincy on the Quincy Center project.

domain: politics


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Posted on March 19, 2021 by Conejo Valley
By JOSHUA SISKIN | [email protected] |
PUBLISHED: March 6, 2021 at 8:29 a.m. | UPDATED: March 19, 2021 at 5:57 p.m.
The Conejo Valley Botanic Garden, a hillside and hilltop horticultural haven, is a community asset highly worth a visit. Rising above the surrounding city of Thousand Oaks, the soil on this modest-sized magic mountain has never been amended, and the plants that thrive there have never been fertilized.
Three factors have contributed to the plants’ well-being. First, they are constantly mulched with tree trimmers’ wood chips. Second, the sloping terrain for the hillside plants and the granitic soil for the hilltop plants ensure that water drains completely away from roots – a critical factor since the least bit of standing water would be fatal to many of the dry climate species on display. Third, the devotion of volunteers to maintenance of the collections, which include plants from the five Mediterranean climate zones around the globe — California, Chile, the Mediterranean Basin, South Africa, and Australia — as well as a butterfly garden, a tranquility garden, a rare fruit orchard, a butterfly garden, an herb garden, a salvia garden, and a fern.
The Conejo Valley Botanic Garden (CVBG) is open seven days a week from 8 a.m. to 6:45 p.m. It is located at 400 West Gainsborough Road, Thousand Oaks. The Kids’ Adventure Garden is open from 11 a.m to 3 p.m. on Sundays. A plant sale is held from 9 a.m. to 12 noon each Wednesday. Admission is free. Dogs on leashes are allowed. Masks are required.
Speaking of the power of mulch, 10 years ago when I visited CVBG there was a vast expanse of weedy black mustard (Brassica nigra) that I never would have dreamed could be redeemed. Yet here we are a decade later and it has been transformed into a thick blanket of black, white, and purple sages. I asked Steve Davis, a landscape architect who helped design the initial garden, how this was done and he cited mulch and hand pulling as the two strategies employed for elimination of the mustard.
As for the beneficial effect of sloping terrain on the growth of the plants at CVBG, I bring Anisodontea x hypomandarum as proof. I have never seen this plant live for more than a few years in anyone’s garden. Even if it gets nothing but winter rain, it is susceptible to root rot where soil is not perfectly drained. But here, in the South African section, it has reached enormous size and is the picture of health after many years in the ground.
Another example of the power of this location is an Australian peppermint willow (Agonis flexuosa). I was astonished to learn that this glorious tree was planted only 27 years ago from a 15-gallon container. Its girth and muscular limbs make it look like it has been there for a century at least. Its symmetry is matched by a nearby specimen of the Queensland bottle tree (Brachychiton rupestris).
Alstroemeria highlights the Chilean section that has only recently been added to the Conejo Valley Botanic Garden. The Alstroemerias we see in our gardens are hybrids between winter-growing Chilean species and summer-growing Brazilian species. These hybrids are virtually evergreen, experiencing only a brief winter dormancy period. Alstroemerias can survive drought and neglect due to their sustaining underground rhizomes. Rich in starch, these rhizomes are part of the diet of the indigenous peoples who live within the Alstroemeria’s habitat. Angel’s trumpet (Brugmansia) is another Chilean selection.
The animal which gives Conejo (meaning “rabbit” in Spanish) Valley its name is the only major pest to contend with at CVBG. Chicken wire encircles plants known as rabbit munchables.
Generally speaking, plants with strong fragrances or flavors, including rosemary and pungent sage species, are not eaten by rabbits. Lomandra, an Australian grass with chartreuse and lime green foliage, is also immune to rabbit ravages. Alstroemeria contains a toxic chemical that causes some animals to stay away while others become ill from its consumption. Not taking any chances, CVBG’s recently planted Alstroemerias are surrounded by chicken wire.
One of the most eye-catching species from South Africa is Euphorbia esculenta. The plant is currently at peak bloom and its flowers at a distance look like daisies. I learned that it is easy to grow and I only wonder why we don’t see it more often. Davis told me that many of the selections on display have been planted with the homeowner in mind. The plants are not only meant to be looked at but are also selections that, not typically seen, will serve admirably as durable choices for the average garden.
To this end, several infrequently seen rosemary cultivars have been planted. An example is ‘Tuscan Blue,’ whose stems shoot up in all directions, producing a pyrotechnic effect. The stems are studded with royal blue flowers. It would make a wonderful complement to ‘Torch Glow’ bougainvillea, a cultivar whose magenta bracts are festooned along shoots that grow out in all directions in a manner that mimics the grown habit of ‘Tuscan Blue.’
Two germanders (Teucrium spp.) are looking brilliant at the present moment at CVBG. Creeping germander (Teucrium cossonii), not exceeding six inches in height, spreads out to three feet, is cold hardy and drought tolerant. Lavender to purple flowers are seen throughout spring and summer but even in its present flowerless state it is something to behold due to its small, daintily scalloped, sea-green foliage. Bush germander (Teucrium fruticans) is a shrub that can grow up to five feet tall and wide. Blue flowers contrast nicely with silvery-gray foliage.
No selection for dry shade is more sensible in our part of the world than hummingbird sage (Salvia spathacea). It grows under oak trees and can go the entire summer without water although it will stay greener in the hottest weather if occasionally soaked. Thick upright magenta flower clusters are accompanied by foliage that emits a pleasant scent when crushed. Hummingbird sage is distinguished from other native sages by its rhizomes, the same structures that allow bearded iris and agapanthus to spread. You can plant hummingbird sage in the shadiest part of your garden and, as long as the soil is consistently mulched, it will just grow and grow and grow.
I was met at the entrance to CVBG by Beverly Brune who, together with Robin Pakorski, has been responsible for fundraising for development of the 33-acre site. I was introduced to Carl Zhu who coordinated a drone flight over the area as a project for local students. The drone photographed tree canopies to evaluate them for carbon dioxide uptake. Trees absorb carbon dioxide through their leaves for photosynthesis, a process that on a large scale reduces air temperatures.
Zhu is responsible for the aviary filled with finches in the Kids’ Adventure Garden. His wonderful photographs are to be found on the ConejoValleyBotanicGarden.com website, which was designed by his daughter. Thanks to Steve Davis for guiding me through the garden and to Jim Allyn for inviting me to visit.
Tip of the Week: If you are pondering low-maintenance formal or informal hedge possibilities, consider the California native lemonade berry (Rhus integrifolia) before making your final decision. Lemonade berry has undulating, leathery sea-green foliage and dense clusters of pink and white flowers followed by utilitarian fruit. To be precise, the fruit should not be eaten whole but can be masticated or sucked for its juice, after which its pulp should be discarded.
Soaking the fruit in cold water also makes a tart and refreshing summer drink. In fact, all plant parts are edible and/or medicinal, as attested to by the many Native American tribes who relied on it for a variety of uses. The only caveat is to experiment with this plant in small quantities for its edible and curative properties since some people experience allergic reactions to it.
Lemonade berry is in the sumac family, with cousins such as poison ivy, poison oak, cashew, pistachio, mango and Brazilian and California pepper trees, all of which are distinguished by turpentine-scented sap. Although it can grow to a height of 10 feet, with an equal or greater girth, lemonade berry may be kept to half that size with occasional pruning. Some people have a dermatitic reaction to contact with its sap so, to be safe, wear gloves while pruning it.
Please send questions, comments, and photos to [email protected]
Hero image – Hummingbird sage (Salvia spathacea). (Photo by Joshua Siskin)
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domain: agronomy


The Columbus Blue Jackets (15-10-2) are in Philadelphia to face the Flyers (11-12-2) tonight at the Wells Fargo Center. Puck drop is scheduled for shortly after 7:00 PM ET.
Following a 9-6 loss to the Calgary Flames at Nationwide Arena on Tuesday night, the Jackets are
With both the Columbus Blue Jackets and Minnesota Wild entering Thursday's contest with identical 14-8-2 records, it didn't take a mathematician to put together the fact that one team would emerge victorious and break that tie following their matchup.
In a battle between two of the
The Columbus Blue Jackets and Minnesota Wild meet tonight in a matchup between two teams with identical 14-8-2 records. Puck drop is scheduled for shortly after 7:00 PM ET.
Despite both teams having 30 points, these two clubs enter tonight's game in different spots in their
The Columbus Blue Jackets (13-8-2) will visit the Detroit Red Wings (10-10-3) tonight in a matchup between two clubs that are seemingly trending in the right direction. The season series between these teams is split 1-1 with the road team victorious in both contests. Puck
The Columbus Blue Jackets and Toronto Maple Leafs met for the second time in five days, this time at Nationwide Arena on Friday evening. After a 4-2 loss in Toronto on Monday night, the CBJ had all week to think about what needed to change
The Columbus Blue Jackets (12-6-2) head north of the border tonight to take on Toronto Maple Leafs (14-6-0) in first of two games in five days between the two clubs. Puck drop is scheduled for shortly after 7:00 PM ET.
Although it's usually tough to get
The Columbus Blue Jackets (11-6-2), coming off an impressive performance on Thursday night against the Florida Panthers at Nationwide Arena, hit the road tonight to take on the Carolina Hurricanes (8-7-3). Sitting atop the Metropolitan Division, the CBJ will look to keep their good start
Thursday afternoon, ahead of the Columbus Blue Jackets' matchup against the Florida Panthers later in the evening, it became known that the club would be without two of their best players: Artemi Panarin and Sergei Bobrovsky. With a number of players under the weather, Thursday
The Columbus Blue Jackets (10-6-2) and Florida Panthers (7-5-3) face off at Nationwide Arena tonight in a game featuring two teams that are starting to hit their stride. The puck is scheduled to drop shortly after 7:00 PM ET.
The CBJ enter this matchup against the
The Columbus Blue Jackets have won two games in a row and have picked up at least a point in six of their last eight contests. After a win last night in Washington, they'll be back at Nationwide Arena tonight to face the New York


Abstract—This article uses dynamic Bernoulli-Euler beam theory to establish a footbridge analysis model with several elastic supports and uses equivalent spring representation of pier to state the elastic impact. By adopting the Semi-analytic method, calculates multi-span footbridge natural vibration frequency and vibration mode. The orthogonality of mode shapes of the analytical model was verified. Then, the modal superposition method is used to solve the lateral vibration response of footbridges under the action of pedes-trian load. Finally, the calculation results are compared with the finite element simulation results. It shows that the theo-retical method is consistent with the finite element simula-tion results. This method can be used to study the dynamic response of multi-span footbridges under pedestrian load and provide a theoretical basis for the rational design of pedestrian bridges.
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text:
A Digital complaint and reporting system for gender related violence in Peru
Ochoa Yañez, Diego Daniel
Silva Bocángel, Jean Marco
Gonzalez, Paola A.
MetadataShow full item record
In this article, we developed a low-cost digital innovation to improve the process of handling legal complaints for cases related to gender violence, especially those related to femicide. Gender-related violence or femicide has been a widely spread health problem throughout the world (refs). In Peru, this violence affects approximately 30-40%(?) of women at some time in their lives (refs). In 2020 (?) the National Police registered approximately 222 234 complaints related to family violence; 30% of these cases were registered through the Women's Emergency Center, a center for women requesting judicial, psychological, and social help. Studies have shown that many women are discouraged to report violence and harassment because the complaint system are often lengthy and ineffective. At the start of this study, we found that several steps of this system were still based on a paper-based system and often prone to error and delays. For this reason, we propose a a low-cost digital solution to improve the registration process of these complaints. The solution presented consists of four components: 1) A mobile application exclusively designed and targeted to women victim of violence, 2) A web application, aimed at those responsible for the managing the registration process of the complaint, 3) Back-end, and 4) Integration with devices of early warning. The proposed solution was piloted and validated with a small sample of 15 women between the ages of 19 and 43 who were victims or witnesses of gender-related violence, an officer from the National Police of Peru and a social worker from the Women's Emergency Center.20 simulations of complaint registers were carried out using the proposed solution. The preliminary results obtained during the validation phase showed that the average time used to register the complaints was and the users’ perceptions of the proposed system revealed that the system could provide transparency and ensure protection in the complaints process.