We, the United States of America, have been conquered! No, not by a foreign country, not by the Communist nor the Islamic, although those threats still exist. We have been enslaved by our own people and apparently the majority of Americans are supportive of the conquest.
The conquest gained ground 40 years ago when the Supreme Court legalized abortion, preventing the States from enforcing their laws against it. Now over 55,000,000 children have been murdered in their mother’s own womb.
In addition there is the relentless prosecution by the ACLU and the Courts to remove any mention of God in the public square.
The final nail in our nation’s coffin has been hammered in by the radical homosexuals. The following overview will give you an idea of what has happened. It is not easy reading!
While this article deals with radical, activist Homosexuals we must acknowledge that they are not solely to blame for the explosion of sexual sins.
In fact heterosexual adulterers and fornicators have caused far more danger and tragedy to society than the homosexuals, in part because there are more of them. While the homosexual caused part of the AIDS epidemic has affected and killed in the millions, the adulterers and fornicators have caused TENS of millions of deaths by abortion. Also to be counted are the deaths caused by abortions by those in traditional marriages, e.g., parents who do not want to raise a handicapped child and those parents involved in in-vitro fertilization (IVF) by abandoning or destroying the embryos not wanted. This includes couples using abortifacient birth control.
The charges of bigotry, homophobia and hatred are usually leveled at those that proclaim that homosexual acts are sinful and dangerous. The truth is that we who do so have, in effect, a more loving concern for the homosexual that is involved in the deadly life style. In fact those who affirm the homosexuals like the ‘Parents and Friends of Lesbians and Gays,’ and others who ‘want to be fair’ are, certainly not on purpose, really showing a complete disregard for their lives.
Consider, would you affirm an alcoholic, a drug addict, an adulterer, a gambler or a prostitute to continue in their deadly pursuits? No, if you loved them you would do your best to get them to stop their death run. It is the ‘Friends’ and ministers, who state the Bible doesn’t condemn homosexuality, whom I believe are responsible for their high suicide rate.
The homosexual knows he is involved in a perverted, self-destructive sexual act; he knows it’s wrong and wants to be set free, but he has these ‘friends’ saying it’s ok, it’s normal, God made you that way. The homosexual says, ‘Here I am, suffering from higher rate of physical disease, STD, mental illness, suicide risk, substance abuse and early death and my ‘friends’ say I’m ok!’
As bad as these ‘Friends’ and ministers are, the worst are those who mandate their acceptance such as the governments, the military and businesses. Their actions give an official, but false legitimacy. The action of President Obama, former Sec. of Defense Gates and former CJCS Admiral Mullins pushing the homosexual agenda on our military forces is a disgrace.
The same applies to the business corporations that require normal employees to attend ‘reorientation/diversity’ classes. And the worst of the worst are those social agencies that allow children to be adopted by same sex couples, ministers that perform a marriage ceremony for them and educators whose agenda is to pervert the minds of school children with their ‘gay friendly curriculum’.
Sadly, there is great acceptance of homosexuality in America. A reason might be the sexual promiscuity of heterosexuals. They are filled with the impurities of their own immoralities- what right do they have to point their finger? The epidemic of oral sex by heterosexuals made popular by Monica Lewinsky and the President of the United States Bill Clinton has had a devastating effect on our society. Apparently now, anything goes!
THE FOLLOWING HEADINGS PROVIDE AN OUTLINE FOR UNDERSTANDING HOMOSEXUALITY AND THE GAY AGENDA:
CAUSES OF HOMOSEXUALITY:
- Excessive exposure to pornography and other sexual images and practices that begin to demand more hardcore sex resulting in moving to homosexuality.
- Sexual/Physical abuse by family members, ministers/priest, sport coaches and others. These pedophiles could have been abused themselves.
- Lack of a father present in the family. Whether he has abandoned the family or a workaholic, the effect can be the same.
- A father who was not affirming (same for the daughter as the son).
- An overbearing mother.
- Lesbians- Hostility to men (for some a valid reason since there are many deadbeats out there).
- Divorced parents.
- A mother with a revolving door of different ‘father figures’.
HELP AVAILABLE TO CHANGE:
- Richmond Set Free
- Exodus Global Alliance
- Restored Hope Network
- Parents and Friends of EX-Gays & Gays
- Ministers/Counselors who are in touch with God
- National Association for Research and Therapy of Homosexuality (NARTH)
- American Association for Christian Counselors (AACC)
DEVASTATION CAUSED BY HOMOSEXUALS:
To SELF– perversion, oral/anal sex, fisting, ‘glory holes’, public baths, MSM, public solicitation, pedophilia, cunnilingus, STD, HIV/AIDS, suicide, mental disorder, physical disease, early death (30 years), bestiality and broken marriages. If one has any doubt of the depraved society of the homosexuals see the videos of the Gay Pride Parades and the San Francisco public baths and read the Gay Manifestos’- A Gay Manifestos 1970 by Carl Wittman and The Gay Manifestos by Michael Swiff published in GAY COMMUNITY NEWS, Feb. 15- 21, 1987).
To OTHERS– contagion, lowering of moral values, danger if they contribute to the blood supply.
Catholic Church- bankrupt diocese, hundreds of millions of dollars paid out, fear and distrust of priest and loss of their spiritual status.
Dollar Cost, including the cost of research for the cure of AIDS that could have gone to nonpreventable diseases. The homosexual threat to liberty-prosecution of ‘hate speech’ enforced by the courts resulting in apologies to those offended, reorientation classes, fines and jail sentences.
SUPPORT IN VIRGINIA TO THE HOMOSEXUAL COMMUNITY:
Virginia Beach Government- One of the first normal governments to ‘protect’ homosexuals (1995). Alexandra, Arlington and Charlottesville were first.
University of Richmond-They are so committed they promote and allow gender-inclusive housing options, gender-inclusive restrooms and a queer community. The U of R homosexuals say they have reclaimed the term ‘queer’ from its historical use as a put-down and redeployed it as a means of empowerment.
VCU also has its Equity and Diversity Committee which has a five year ‘diversity’ plan.
Richmond Area James River Presbytery– (Presbyterian Church- USA). They vote to accept homosexual clergy, a number of churches are leaving.
Richmond Federal Reserve Bank – The Bank President Jeffrey Lacker flew the homosexual rainbow flag for a month over the Fed Bank in honor of the homosexual bank employees.
Governor Bob McDonnell and Lt. Governor Bill Bowling show their support for the appointment of a homosexual judge. Bowling stated-‘Appointments should be made on the basis of merit and qualification, and no other consideration, personal or political should be a factor.’ Apparently Character need not be considered. Virginia Senators Kaine and Warner along with the majority of the Democrats support the homosexuals.
Virginia National Guard-It falls in line with the Defense Dept.
Willow Oaks Country Club– Richmond brings into membership two ‘married’ homosexuals proudly showing their pictures in the monthly magazine. This despite the fact homosexual ‘marriages’ are not legal in Virginia. The Board of Directors was unanimous in their acceptance.
The Richmond Baptist Association– Ginter Park Baptist Church ordained a homosexual. While they were kicked out of the State Baptist, the Richmond Baptist Association voted to keep them in. They were supported by First Baptist Pastor Jim Somerville and Tabernacle Pastor Sterling Severns.
The Richmond TIMES-DISPATCH- Their news and editorial support for the homosexuals is so significant that you would think they were a subsidiary of the WASHINGTON BLADE. The T/D also supports abortion and cohabitation.
GOVERNMENT LAWS & COURT CASES:
Until 1961, homosexual acts were outlawed in all 50 states. In 1986, the Supreme Court upheld the right of the States to adopt sodomy laws (Bowers v. Hardwick). Chief justice Warren Burger wrote: “To hold that the act of homosexual sodomy is somehow protected as a fundamental right would be to cast aside millennia of moral teaching.” In 2003 the Court did cast aside ‘millennia of moral teaching’ in Lawrence v. Texas over ruling Bowers v. Hardwick.
In 2013 in a Virginia case, MacDonald v. Moose, the Eastern US District Court reversed MacDonald’ sodomy conviction using the Lawrence v. Texas decision. However the remainder of the Virginia Code 18.2-361 (A) that prohibits bestiality by criminalizing the carnal knowledge “in any manner [of] any brute animal” was not challenged by this decision.
Now the Supreme Court has heard arguments to sanction homosexual marriages. Twelve States have authorized homosexual marriages.
Destructive behavior should be punished by the civil government- adultery, fornication, homosexuality, prostitution (both homo and hetero), pedophiles, rapist and bestiality.
GOD AND THE HOLY BIBLE:
Our nation was founded by Christians and others who believed in God. This gave birth to our great nation, one with the greatest freedom, liberty, stability and prosperity ever known. I believe God has blessed us because our founders and we looked to Him in forming our culture. Now it is different. Now we deny God and want nothing to do with his laws. Nowhere is this more evident in our rejection of His demand for sexual purity, particularly the perverted vice of homosexuality. The assault by the radical homosexuals and their allies, the ACLU, university professors, the Democratic Party and the media has been devastating to our nation’s culture. Now right is wrong and wrong is right! It remains to be seen what the final act brings.
But we do know what God has demanded. And we do know what the consequences will be. What are some of the words God uses in referring to homosexuality? Abomination, detestable act, indecent act and unnatural function are the answers and none of them are good. While God has killed many people for various sins, we need to consider the most horrific event, after the FLOOD, the destruction of Sodom and Gomorrah.
The most important thing in a person’s life is to know where he will go at the point of death. We know we need to be ‘born again’ to be with the Lord. But there are a number of Scripture passages we need to consider.
RUN AWAY FROM SEXUAL SIN! NO OTHER SIN SO CLEARLY AFFECTS THE BODY AS THIS ONE DOES. I Corinthians 6:18a
DON’T YOU KNOW THAT THOSE WHO DO WRONG WILL HAVE NO SHARE IN THE KINGDOM OF GOD? DON’T FOOL YOURSELVES. THOSE WHO INDULGE IN SEXUAL SIN, WHO ARE IDOL WORSHIPPERS, ADULTERS, MALE PROSTITUTES, HOMOSEXUALS, THIEVES, GREEDY PEOPLE, DRUNKARDS, ABUSERS AND SWINDLERS- NONE OF THESE WILL HAVE A SHARE IN THE KINGDOM OF GOD. 1 Corinthians 6:9
And don’t forget the cities of Sodom and Gomorrah and their neighboring towns, which were filled with sexual immorality and every kind of sexual perversion. Those cities were destroyed by fire and are a warning of the eternal fire that will punish all who do evil. Jude 7
AGENDA OF THE RADICAL HOMOSEXUALS:
The homosexuals have learned from the Abortion Industry how to change people’s perception of their evils. The abortionist public relations people came up with the sound bites: It’s my body! (Don’t mention the baby), It’s my CHOICE!, it’s a medical procedure, it’s a decision between the woman and her doctor, trust the women and the best lie of all: it’s just a blob of tissue. Label opponents anti-choice, judgmental, etc. Bring in the power of the government for legality and protection and the Medical Societies (AMA, ACofObGyn).
So the homosexuals came up with:
Gay in lieu of homosexual, alternate lifestyle, loving- stable relationships, fairness, civil rights (compare with racial), never mention oral sex.
Label opponents bigots, homophobic, hateful, religious fanatics.
Solicit support and depend on the media, Hollywood, mainline religious denominations, universities and teacher labor unions for support.
Bring in the government for legality and protection and punishment for those who oppose homosexuals.
Next to be accomplished:
Make the Boy Scouts of America accept homosexual leaders. BSA has approved homosexual boys for membership. How they will rework the Scout Oath that says they will be ‘morally straight’ remains to be determined.
Make the Supreme Court to ok Homosexual marriages.
Silence the Southern Baptist Convention and their conservative pastors. It should be noted that all Baptist churches are not connected to the Southern Baptist denomination. For example, First Baptist Church-Richmond (Monument Ave.) ended their relationship with the Southern Baptist years ago although they still send support to the SBC International Mission Board.
Use the IRS to remove tax-exemption for the ministries that don’t conform. Declare Biblical text hate speech- assess fines and prison sentences.
HOMOSEXUAL ORGANIZATIONS & SUPPORTERS:
The North American Man Boy Love Association is a pedophile and pederasty advocacy organization that works to abolish age of consent laws criminalizing adult sexual involvement with minors and for the release of all men who have been jailed for sexual contact with minors that did not involve coercion. It has been defended by the ACLU.
The United States Supreme Court, the Executive and the Congress.
ACLU, The Democratic Party, Hollywood, TV
President Bill Clinton and General Colin Powell- ‘Don’t ask, don’t tell’. President Barrack Obama, Sec. of Defense Gates & Panetta and Admiral Mike Mullen-Complete acceptance of homosexuals in the military.
There are too many dedicated homosexual support organizations to list. Up to 77% of the American public are said to be supportive.
Some homosexuals insist that they were ‘born that way’. All of us are ‘born that way’ so to speak- that is with a sinful nature. Whether its heterosexuals or homosexuals you must to be BORN AGAIN, by trusting in Jesus Christ as your personal Lord and Savior. This involves repentance, faith, trusting and acknowledgement of Jesus Christ, there is no other way. God cares and cares redemptively for every one of his creatures.


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More often than not, people sign up for online casino games without paying any attention whatsoever to the suitable legal restraints. Online casino video games have become preferred given the ‘surge’ of the Internet in the early 1990s. Before you enroll in mobile roulette, blackjack, or any of your favored online gambling casino video games, you ought to ensure that you are well educated regarding the specifics of what the law in your country dictates. Many individuals had no option but to believe that gambling was a dirty game where con artists and addicts represented their unhealthy habits. Therefore, it is crucial to be well educated on what nation enables gambling to ensure that the games you play are legally identified.
In the United States, even though there has been a boom in gambling and pc gaming company, no state has carried out laws licensing online gambling on profit-making sites. The Gaming Control panel is responsible for any licensing, possession regulation, control, and surveillance of all casino sites. Unlike in the USA, slot online gambling and wagering are entirely legal in Australia. That indicates that any person in Australia can play mobile live roulette, blackjack, or any one of their favored online casino video games. Based on legislation effected in 2006, casino gambling, or any other communication innovation reliant gambling, is unlawful in the Russian Federation.
1.In the Indian state of Maharashtra, online gambling is altogether unlawful.
2.In addition to backgammon, Israeli regulation does not allow online gambling activities.
The federal government of France permitted web-based gambling to be lawfully run in 2010. However, games of chance, such as the video clip ports and roulette, remain unlawful. This was chosen to remove any cases of gambling addiction. British regulation permits many forms of net gambling. Casino video games such as live roulette, blackjack, and the slots are well within the bounds of state regulation. Many posts and files offered online provide well-formatted details relating to the validity of online gambling in different states or nations.
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domain: entertainment


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CL McEachern - The music of the soulOver the years, he played with artists from Hip-Hop, Reggae, EDM, Hard Rock, Folk and every genre in between.Enjoy and share.
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The baisakhi kala utsav has been conducted by pune’s The punjabi cultural association which has been concluded with great enthusiasum.
The main attraction to this program was Narendra singh sachadev and his co star nawab punjabi, Raany kaur mandeep singh sindhu and they won d heart of public by performing the famous dance form of Punjab Bhngada , and Gidda.
The President of The Punjabi cultural association Mr .Satish Chadha and Vice president Mr.Ashok Agrawal , Arun Chopra sec. Vijay agarwal and others members were present for the event. President Mr .Satish Chadha thanked all the members and artists. The cultural audience who have come from Pune city participated in the programme with the enthusiasm.
The punjabi cultural association has been working since 1955 and they have participated in various social programmers like providing books /study material to needy Students, providing medical facilities to senior citizens .The President of The Punjabi cultural association Mr .Satish Chadha Said to people of Pune to make pune pollution free city.
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Goa Institute of Management hosts its Grand Alumni Meet
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Proposals for a single-payer health plan have reared their ugly heads again.
Some legislators are trying to resurrect SB 562. The Legislature’s own analyst says this would cost at least $400 billion. California is already the highest-taxed state in the country. Vermont started to implement such a plan and then reversed when it realized they couldn’t afford it.
In all states where proposals were on the ballot, they were soundly defeated. Meanwhile in the U.K., which has such a health plan, it is collapsing. Why? Because they can’t afford it. Cuts to the National Health Service budget in Britain have left hospitals stretched for years. The situation has become so dire that the head of the health service, Simon Stevens, is warning that the system is overwhelmed. Is this what you want for your family? Then just send your paycheck to the state and get on with it.
What do you think about this? Submit your letter to the editor via this form
Want to read more Letters to the Editor? Click here.
View more on The Mercury News

domain: economics


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Author details Centre for Health Communication and Participation, Australian Institute for Primary Care & Ageing, La Trobe University, Bundoora, VIC 3086, Australia. Norwegian Knowledge Centre for the Health Services, PO Box 7004, St. Olavs plass, N-0130, Oslo, Norway. Health Systems Research Unit, Medical Research Council of South Africa, Cape Town, South… (More)
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domain: agronomy


Rally for Education about Sustainable Development
The Rally for Education about Sustainable Development (French: Rassemblement pour l'éducation au développement durable, REDD) is a political party in Mali. It contested the presidential election on 29 April 2007 with the candidate Sidibé Aminata Diallo, who took seventh place with 0.55% of the vote. Diallo is the first woman to ever contest a presidential election in the country.
- "Présidentielle au Mali: la Cour constitutionnelle valide la réélection de Touré", AFP (Jeuneafrique.com), May 12, 2007 (in French).
- Almahady Cissé, "A Presidential Election That Breaks With Tradition", Inter Press Service (allAfrica.com), 24 April 2007.
- Participation status of political parties in the 2013 legislative elections Ministry of the Interior


Waldman, who has 16 years experience in managing North American-listed companies with operations in China, has rejoined the company after taking a one-year sabbatical. He previously served as Silvercorp's corporate secretary from 2007 to 2012.
Prior to this, he held a senior management position with an NYSE-listed electronics manufacturer, according to the China-focused silver miner's statement.
In his new role, he will be in charge of the corporate head office in Vancouver, British Columbia, and will be responsible for investor relations, corporate communications and other corporate matters.
Last week, Silvercorp Metals said it had identified and moved to address fraudulent contractor practices that resulted in diminished head grades at its China mines, with its new approach already leading to improved head grades, the Vancouver-based miner revealed in a production update for the Ying mining district.
The China-focused, low-cost silver producer, in its efforts to adapt to the new price environment, has made a point of reviewing its operations in a quest to identify any potential cost saving measures and in so doing, discovered a practice among mining contractors in the Ying mining district of China that has resulted in a misleading dilution of grade.
A key finding of the silver producer's review was that contractors at the site were engaged in dilutive practices, which contributed to head grade decline as reported in the miner's two most recent quarterly financial reports.
Silvercorp has taken immediate action to address the situation, modifying the method of calculating the actual amount of ore mined by contractors. In the short period in which the new method has been in place, it said silver and lead head grades at the SGX mine in the Ying mining district improved by almost 35 per cent and 21 per cent, respectively, with an approximate 45 per cent reduction in ore production (a 25 per cent reduction in overall silver metal production) compared to July of this year.
The company is forecasting that during this time of transition, ore production with improved head grades may remain at a reduced level for one to two more quarters. Nonetheless, the silver miner expects the new method to lead to long term cost savings and improved head grades.
Disclosure: I have no positions in any stocks mentioned, and no plans to initiate any positions within the next 72 hours.


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- KCII Radio - http://kciiradio.com -
St. Mary’s (Riverside) Labor Day Celebration
Posted By News On @ 11:25 pm In Calendar, Today's Local Church News | Comments Disabled
Title: St. Mary\’s (Riverside) Labor Day Celebration
Description: St. Mary’s Catholic Church in Riverside is having a Labor Day celebration Monday. They will serve food from 11:00 to 2:00 with carryout available. There will also be games, a farmers market, a raffle, and auction. For more information, call Dee Simon at 319-351-0300.
Article printed from KCII Radio: http://kciiradio.com
URL to article: http://kciiradio.com/2011/08/st-marys-riverside-labor-day-celebration/
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|australia lockdown pets||0.04||0.9||4872||34|
The RSPCA said the boom in pet ownership could turn into a "crisis" for those animals once their owners returned to work after lockdown and could no longer lavish so much attention on them. RSPCA pet welfare expert Samantha Gaines said: "Many of our pets are now used to having us around all the time, while others have never known any different.Is there a lockdown in South Australia?
South Australia began the strict six-day hard lockdown on Wednesday. The measures are among the toughest in the world, with only countries such as Argentina, Israel and Venezuela enacting such extreme restrictions.Who is buying pet food during Lockdown UK?
Helen Warren-Piper, general manager of Mars Petcare UK, which makes Pedigree and Whiskas, said: "We recognise retailers are experiencing unusual demand for pet food during lockdown." Under-35s account for 59% of new pet owners, while 56% of those buying a pet for the first time have children at home, the PFMA says.Is it illegal to walk your dog in lockdown?
Police said 'you cannot leave the house to walk the dog or to exercise' in a tweet on Wednesday, outraging many who siad it hurt hurt animal welfare. People who are found flouting directives can be fined up to $1,000 - including for walking their dog during the lockdown.

domain: pet


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|Born||in Kansas City, Missouri, USA|
|Died||in Burbank, California, USA (heart attack)|
|Birth Name||Ubbe Ert Iwwerks|
|Height||5' 9½" (1.77 m)|
Mini Bio (1)
Ub Iwerks worked as a commercial artist in Kansas City in 1919 when he met Walt Disney who was in the same profession. When Disney decided to form an animation company, Ub Iwerks was the first employee he had due to his skill at fast drawing as well as being a personal friend.
When Charles Mintz raided Disney's animation studio and stole the rights to their character Oswald the Rabbit, Ub was the only associate to remain with Disney. He served as the principle animator for the first Mickey Mouse shorts and Silly Symphonies. Iwerks was so prominent in the production of these shorts that it was speculated that Ub was the dominant force behind the success of Disney Productions. The combination of Iwerks' rising ambitions, occasional differences with Walt and a tempting deal with Pat Powers to finance his own studio prompted him to break away in 1930. His studio was never a tenth of the artistic or financial success that Disney was. He simply did not have the creative talents of his partner and his characters, Flip the Frog and Willy Whopper were rather dull failures. His studio was closed in 1936 when Powers withdrew his support. He worked for Columbia starting in 1938 and worked for two years until he decided to return to Disney. The two men never commented on their renewed relationship but the reunion was mutually beneficial. Iwerks was able to abandon animation and concentrate on technical development which helped create many of the special effects that the Disney company excelled in for decades, especially concerning the live action animation combination sequences in Song of the South (1946) and Mary Poppins (1964).
- IMDb Mini Biography By: Kenneth Chisholm <email@example.com>
|Mildred Sarah Henderson||(5 January 1927 - 7 July 1971) ( his death) ( 2 children)|

domain: artistic


Brand: For Your Water
Product Code: FYW-CL520
Availability: Special Order This item may need to be special ordered due to limited on-hand stock
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The CL500 Essential Series Top-Load Coolers provides you with the same heavy-duty framework and internal hardware of the high-end coolers traditionally leased and sold through by professional water delivery companies, but at a significant cost savings. While they are durable enough to withstand years of abuse and heavy traffic, they also have a sleek, modern design that fits perfectly into any home or office decor, and a whisper-quite running sound that will ensure they go unnoticed except when they are in use.


If you own a Linksys router and are unsuccessful in inputting the password for it via the control panel, then you aren’t the only one.
This is a common problem many users face, and because of that, we’ve decided to create a detailed article on how to fix it.
What can I do if my Linksys router is not accepting the password?
My Linksys router will not let me log in
1. Check if you can ping 192.168.1.1
Start the troubleshooting by wiring a computer to your router. Just open the Command Prompt to see if you can ping 192.168.1.1. In case of getting five good replies, then your router is not defective or anything.
It’s most likely a glitch causing this, so type 192.168.1.1 on the same command prompt window. Press Enter. This should normally launch a browser and you will get the prompt for username and password.
2. Upgrade the firmware
Various errors happen to routers whose firmware hasn’t been updated. That’s why we further suggest that you upgrade the firmware of your router as soon as possible.
3. Reset Linksys to factory defaults
If the log-in window refuses to accept your password no matter what you do, it’s time to reset the router to factory defaults. To do so, press and hold the Reset button from the rear side of the device for about 10 seconds, until the power LED starts blinking.
Be careful that some older Linksys routers still require you to press and hold the Reset button for 30 seconds to get the same result.
To complete the procedure, unplug the power cable from the router for 30 seconds more. Lastly, plug it back in. This will erase all the settings, so you’ll need to completely redo your configuration.
Linksys router admin password is not working
1. Try multiple password possibilities
You’re most likely trying to get into your router‘s configuration page. You are supposed to enter admin, but the log-in box doesn’t accept it and merely erases what you typed in.
In case of any security gaps, then perhaps someone wirelessly connected to your router and changed your password. Also take into consideration to have changed it earlier by yourself, while the first-time installation.
2. Reset your router to factory default settings
If applying the above steps doesn’t seem to have any effect, turn on the router and wait until the Power light is blinking. Up next, press and hold the Reset button for 10 seconds (or more on some models).
Finally, unplug the router to turn it off, wait 30 more seconds, then plug it in again to turn it back on. This procedure will erase its current settings. Hence, you will need to reconfigure the router settings.
3. Set Auto Login on your browser
If you’re using Google Chrome, select Preferences, then click on Advanced to expand the options. Toggle Allow Chrome sign-in to the On position with the current username and password.
Follow a similar procedure on any browser you might currently use and put a checkmark on automatic log-in to further prevent this situation.
My Linksys router will not let me change password
1. Update Linksys firmware
If you can change any other settings on the router, but not the security, it’s time to update the router‘s firmware.
See it as an operating system for your Linksys wireless router. Your router won’t be able to function properly unless you keep it up to date.
2. Clear the Browsing Cache
Apply this procedure on the particular browser that you’re using. If it’s Google Chrome, press the three horizontal dots on the top right corner. Then, click Clear browsing data on the History page.
At the top, choose a time range or select to delete everything. You could also tick the boxes next to Cookies and other site data and Cached images and files. Click Clear data.
If you also want to clear the auto-fill data in Chrome, follow the simple steps from this useful guide.
3. Fully reset Linksys to the factory default settings
Sometimes, simply rebooting your router will solve the problem. To do this, remove the power cable. Let your router sit for 10 seconds, and plug it back in.
If power cycling the router made no difference, set it up from scratch and see if it’ll let you change the admin password.
Tell us which of these solutions helped you with your Linksys router issue in the comments section below. Also, leave there any other questions or suggestions that you may have.
- Invalid IP address range on Linksys router [EXPERT FIX]
- Fix: Windows 10 can’t Connect to router
- 4 handy VPN tools for Linksys routers to secure your connection


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Battle of the Books
BoB is a District-sponsored competition where grade level teams compete by reading ten books from the designated list then work together to answer multiple choice questions. Our first meeting will be in December and then meet twice a month during January and February moving to weekly meetings March and April.
Angevine Bobcats have done very well at the Battle of the Books for years. We intend to keep that streak going!
Contact email@example.com for more information.

domain: library


The inferiority of women is man-made.”
American author, activist, and lecturer Helen Keller, June 11, 1916 (via womenaresociety)
Reminder that Helen Keller was a bad ass, and the fact that people only remember her as an “inspirational” child is really annoying.
CultureDANCE: Alvin Ailey American Dance Theater
the thing I love about the Alvin Ailey Dance Company is that as soon as you see these shots you know immediately that these dancers are Alvin Ailey dancers just because of the beautiful composition and lines and flexibility and (most importantly) the fact that there are this many Black dancers in one company just sayin’.
<3 Alvin Ailey
they came to umich and i am forever heartbroken that i didn’t get to see them


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Sorted By Most Useful
By Michelle on 09-07-10
Laugh out load; sad and romantc
After reading the reviews written here, I felt I needed to add how much I enjoyed this book. It is a fantastic story of Fawad,a young boy's world in post Blair/Bushes Afganastan. After I read the story I serched Andrea Busfield Biography and she has indeed lived and worked in Afganastan as journelist so must know a great deal about the country. I don't think I can recomend this this book highly enough. It is pure joy, well read by Mark Medows
1 of 1 people found this review helpful
By Alina on 02-01-14
What made the experience of listening to Born Under a Million Shadows the most enjoyable?
The storyline, the characters and the way the story was set.
Who was your favorite character and why?
Fawad, main character.
Was there a moment in the book that particularly moved you?
Not really but the book itself is amazingly set, it has inspired me t start reading more, experimenting with various books.
The author has a great storyline which keeps you the edge, wanting more.
By Elizabeth on 11-29-10
A more lighthearted view
Having read Khaled Hosseini, and others about Afghanistan, all of which I would highly recommend, this is a surprising pleasant change. There are some lovely moments, and some funny characters, and seen from the eyes of a child, it gives you a view that even amidst a war children worry about childlike things and they see the world in a different way. Don't come to this book if you are hoping for 'The Kite Runner'...take it is as it is, a nice story about a young boy, in a war torn country, who thinks about life no differently to any boy his age. The author has captured it well.
By MaryAnne on 10-31-10
Wonderfully balanced book.
I listened to the unabridged audio version of this book during a recent long journey and the miles flew by. The author has hit just that perfect balance between putting across a serious message and making a book entertaining. For a book set in Afghanistan it has some wonderfully humorous momnents too.
Fawad is an 11yr old boy, living with his mother in an aunt's house. His father and two brothers are dead and no-one knows what has become of his sister since she was abducted by the Taliban. They have barely enough money to feed and clothe themselves, with nothing remaining to pay rent. Fawad runs with the local children, begging and stealing money from unsuspecting foreigners.
Their luck changes dramatically when his mother gets work as housemaid to a group of foreigners who share a house. She does all their cleaning and cooking and Fawad is allowed to live there with them. Georgie, James and May are wonderful characters and the interactions are great fun.
Fawad gets work in a local grocery store run by a blind man who is a constant source of insights into life and interactions between people; he has an opinion on everything.
As time passes over a period of about 18 months, there are both happy and sad moments but the book is ultimately uplifting.
The audiobook was beautifully read by Mark Meadows, bringing all the characters to life and I would thoroughly recommend it to anyone who has an interest in global literature.
By Pat on 12-11-09
This is a lovely book which I initially avoided as I thought it would be a pale copy of the Kite Runner. It is a beautiful story, with a range of different people brought together in Afghanistan. I don't know if it is a particularly realistic portrayal of the people and the country of Afghanistan, but it was just what I needed after the images of death, misery and suffering we hear everyday. It is a positive image of the Afghan people working to rebuild their own society, despite the challenges they face.
By ZakiGhiacy on 03-27-10
Born Under a Million Shadows
I was very disappointed with this book. The author has no in depth knowledge of Afghanistan and its people. I very much doubt she has even been there. Names, cultural references and traditions are completely misrepresented. The book is peppered with foul language and she has even managed to get these wrong. This book does not bear comparison with other, better written books such as those written by Khalid Hosseini. Ms Busfield needs to spend time in the country. This book could have been based in Basingstoke.
0 of 1 people found this review helpful

domain: literature


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The elderly are eating every other day so that they can save children’s livesPublished: Oct 11, 2017 Reading time: 3 minutes
A long drought in the Horn of Africa is threatening the lives of 17 million people. They don’t have water and wait in vain for harvest. In this large humanitarian catastrophe, Czechs are helping pastoral tribes in the south of Ethiopia.
Every year, the Bodi tribe from the zone of South Omo in Ethiopia chooses a man from each family for him to drink during half a year litres of a cocktail from cow milk and blood. Soon the chosen ones literally puff up. Everything ends with a ceremony of the men showing off their newly acquired corpulence to the others. And precisely nowadays they lose their only possession and livelihood source. Ethiopia, part of the so-called Horn of Africa alongside Somalia, South Sudan or Kenya, is afflicted by deadly drought. The cows die of thirst and drinking water is already lacking for people, too. They wait in vain for the rain to pour over their fields and for the pasture for their cattle to grow.
According to the World Food Programme, as a consequence of this crisis 8.5 million people in Ethiopia do not have sufficient food. “By saving cattle, we save people. They are dependent on meat and milk, which are now lacking, and undernutrition is getting worse. I witnessed how the elderly are leaving their food for the younger. For example, they were eating once every other day, in order to save the lives of their children and grandchildren. This is how bad the situation is,“ says Michal Przedlacki, a humanitarian worker from Poland, who was at the beginning in the charge of the whole People in Need operation.
“Our main task is now to ensure feed for cattle and water to villages. In some places, we repair the water wells that are broken. In other places though we have to deliver water in tanks, or even by donkey, because there are no roads, “ he recounts. “For example in Jinka, the main city of the region, there is only one hospital with a catchment area of over a million people and this hospital is now getting water from the river,“ he adds. Women and children spend many hours a day walking for the life-giving liquid. Now the sources of water are getting farther. “We do not only give people again water, but also free time. Instead of walking with a container they can instead, for instance, sit in a school bench or take care of their family,“ adds Przedlacki. The Czech organisation is supported by the European Commission’s Directorate-General for Civil Protection and Humanitarian Aid Operations (ECHO).
A big challenge is the language barrier. Individual tribes in South Omo speak their own languages, which are often not used by anybody else. This is why there always has to be a competent interpreter in People in Need’s team. “Not even 5% of the local population here speak Amharic, Ethiopia‘s official language. We even work in a village, where there are the last 91 people who speak their own language,“ says Michal Przedlacki. He spent a few months in South Omo. “The villages where we work are very remote. Often there are no roads leading there, so humanitarian workers spend hours on the road. Temperatures here regularly reach over 40 Celsium degrees. It is a very stressful job. But as a humanitarian worker, I am there where it is necessary,“ adds Michal Przedlacki from People in Need.


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You could be so eager to move into the college dorm that you don’t consider how simple and basic it’s likely to appear, and believe us, they all do. Don’t even get us started on the restricted square footage, which you, by the way, share with another person.
Regardless of the cramped quarters and austere look, this is about to be the backstory to your whole college experience-we’re not going to let you go four years with a boring dorm room. With a little imagination, you can turn your allotted living area into a beautiful home-no matter how restricted your lighting is, how cold your brick walls appear, or how monotonous your furniture appears, we have suggestions for bringing your plain dorm to life. Here are 15 of the best dorm room ideas to turn your blank dorm room design canvas into a real masterpiece.
Indoor Plants: Green Buddy
Including plants on the list of dorm room ideas will make the area seem more cozy and pleasant. Dorm rooms are typically dull, and for those who are always lively and eager, living things can bring some life into dull quarters.
Plants are low-maintenance green buddies that take little space and give color to the entire area, giving it a bohemian air. Many indoor plants, such as snake plants, dracaena, and numerous other species, require little care and may thrive with little light. Because a student’s life is hectic and full of deadlines, choosing low-maintenance yet attractive plant species will quickly brighten up a dorm room.
Buy Huge Mirror
With limited space, installing a full-length mirror on the rear wall of your dorm room is a sensible method to minimize visits to the common hall to check your look. It will also make your room appear larger since the shiny surfaces fool the eyes into believing that the area extends longer.
Starting college is thrilling, but it also means starting over in a new setting without your family and friends. In this scenario, putting up a photo wall not only adds a beautiful aspect to your room. But also makes it seem homier and brings you closer to your friends and family. This photo wall will grow as you collect more memories in college.
Add Twinkling Fairy lights
Twinkling fairy lights can give a space a really warm and whimsical feel. These lights will create a starry appearance on the walls, ceiling, and drapes. There are countless ways to use fairy lights to express your creativity and make the area feel more personalized to your preferences. As a result, they should be on your list of dorm room design decor!
Employ Power Strip
Along with a lack of space, another concern with dorm rooms is a lack of power outlets. For your convenience, you should arrange ahead of time with power strips or extension cables. You may choose interesting, funky extension chord styles that don’t detract from the overall look of your space. This can be one of the essential college dorm ideas that will come in handy during your years.
You may fill an entire wall with magazine clippings and inspiring phrases hence, simplicity is key. A single large piece of framed art adorns your walls without becoming overpowering. If you do this, your room will appear classy and not boring.
You’d be remiss if you didn’t decorate your room the same way you would your clothes for a night out. Texture, such as a faux fur throw blanket, ornate cushions, and a tufted headboard, can dramatically enhance your area.
Go for Chic Rug
Dorm room floors are built of hard and durable material to withstand heavy use, so adding a rug of your choosing is a great dorm room design to make the floor more appealing and comfortable. It may also be used to cover up any damage on the floor. It also provides a splash of color to the space.
Your dorm room ideas will maximize space and make optimal use of it. Pushing the study desks to one side frees up space for other stuff and defines your personal study area. Study tables on one side of a shared space might help with group work and discussions. Adding cushions to the study chair might change it from the unpleasant dorm seats.
Dorm Decor Stuff
Dorm rooms have very little place for extra furniture, but you may put your own personal touch to the walls. By incorporating unique wall decorations, you should experiment with numerous dorm design ideas. Wall art and posters from your favorite artists make a huge statement and add added comfort to the entire area. Walls may be a manifestation of your personality; whether it’s your favorite album or artist, or your favorite book covers or posters, they can assist to add a fun element to your rooms.
Because it is proven that gloomy and dark rooms can harm your mental health. It is best to let as much natural light as possible into your dormitory during the day. To keep you happy and focused in your dorm room, you must have good lighting.
If your dorm room design is not oriented in the direction of natural sunshine. It is preferable to choose light bulbs that seem natural rather than those that emit conventional artificial light. Lighting designs are one of the most fundamental dorm room ideas that may quickly bring life to your areas.


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BY NEWS REPORTER
Eltham Lights Up saw thousands of people flock to the high street to enjoy the annual winter event. There were strolling entertainers including Superman, robots and aliens to keep families entertained – and provide some great opportunities for a selfie
Children from primary schools spent hours crafting lanterns in the form of planets and the Milky Way for the residents to admire during the annual Eltham Lights Up lantern parade.
Residents were entertained with music and activities throughout the three-hour event as schools and community groups took to the streets to showcase their dancing and singing skills.
Councillor Denise Scott- McDonald cabinet member for culture, creative industries and community well-being, said: “Eltham Lights Up was a great evening and showed off the newly-developed high street, which was packed from top to bottom.
“Once again, our fabulous police and fire crews provided great company for the young people who were lucky enough to get a chance to sit behind the wheel of a fire engine and police car
Please support your local paper by making a donation
Please make cheques payable to “MSI Media Limited” and send by post to South London Press, Unit 112, 160 Bromley Road, Catford, London SE6 2NZ
Housing Secretary Robert Jenrick has encouraged everyone in the country who can afford to do so to buy a newspaper, and told the Downing Street press briefing recently: “A free country needs a free press, and the newspapers of our country are under significant financial pressure”.
So if you have enjoyed reading this story, and if you can afford to do so, we would be so grateful if you can buy our newspaper or make a donation, which will allow us to continue to bring stories like this one to you both in print and online.


Report: Digital Disaster Resilience in State and Local Government
This Government Business Council (GBC) Issue Brief explores why public sector organizations should adopt a thoughtful and deliberate model of shared services that is laser-focused on standardization, centralization and the citizen experience.
Maintaining perspective on the entire array of government functions during a disaster
Key tools and technologies that lessen the burden on emergency preparedness and agency leadership professionals
Examples of integrating first response with secondary disaster response responsibilities
Ready to transform the way you work?
Laserfiche delivers rapid ROI for your organization. Get in touch to see what Laserfiche can do for you!


text:
Course contents *
Regional Planning is a cross-disciplinary interest field. It concerns theories and methods regarding development planning based on an insight to related social, economic and environmental processes and phenomena. The subject deals with planning at many levels, from local to the national and, in turn to international. Special emphasis is placed on theories and methods that are applied to the shaping and evaluation of urban settlements in a regional context.
The complex nature of regional development and its planning requires that connectivity and interplay between different components of planning determine theoretical and methodological approach and choice investigatory techniques. Focus of inquiry is in the organisation and substance of national, regional and municipal planning in globalisation context. Theoretical and methodological interest areas of special significance include analyses of goal conflict and the implementation of plans in negotiation based economies.
Contents of thesis work
A thesis work shall constitute the equivalent of 20 weeks of full-time study. It shall contain: clear problem formulation, well developed theoretical framework, operational, methodological approach, rigorous analyses, clear result/finding/outcome presentation and comprehensive discussion of the result/finding/outcome in relation to problem, theory, method and way forward.
Suggestions for a thesis topic may be proposed by the student, a potential supervisor or an external interested party. A synopsis by the student should propose the interim idea of problem, context, theory, method and anticipated result of the thesis on at least one A4-size sheet. The department will appoint supervisor and examiner for the student’s research area. The supervisor and the examiner may in exceptional cases, after decision by the head of the department, be the same person.
In consultation with the appointed supervisor, the student develops the synopsis and prepares a time and activities plan for the thesis work. An interim, and thus over the thesis period changeable, contents list of the envisaged chapters and sections of the thesis should accompany the plan. In partial fulfilment towards approval of the thesis work course, the student’s defence of her/his own thesis at a public final seminar must have been carried out to the satisfaction of the examiner.

domain: geography


LCOA® designs, develops, and manufactures precision quality backup and entry solutions used in the manufacturing of printed circuits boards.
LCOA® has been located in Southern California since its inception and has become the Northern American leader in backup and entry sales while also selling products on five continents and in over 15 countries around the world.
Today, LCOA® supplies drilling solutions to many of the largest PCB manufacturers in the world.
LCOA® is the technological leader for backup and entry – all to help drill a better hole.
Superior HWQ with increased stack height and extended tool life.
Paper-based backer with excellent HWQ and reduced temperatures and burrs.
MDF backer board used in lower technology applications.
Specialty products showing significantly reduced image transfer and burrs. Minimizes need to rework
Excellent HWQ, with no phenolic resins, designed for PCBs with an emphasis on burr reduction.


text:
Please use this identifier to cite or link to this item:
|Title:||The natural history of cardiovascular risk factors in health professionals: 20-year follow-up|
|Author:||Veiga Jardim, T.|
Lima Sousa ,A.L.
Rolim Povoa, T.I.
Sebba Barroso, W.K.
Veiga Jardim, P.C.B.
Factors de risc en les malalties
Risk factors in diseases
|Abstract:||Background The knowledge of the presence and evolution of cardiovascular risk factors in young people may significantly contribute to actions to modify the natural history of these risks and prevent the onset of cardiovascular disease. Objectives To assess the presence and evolution of cardiovascular risk factors in health professionals over a 20-year period. Methods A group of individuals was evaluated when they first started graduate programs in medicine, nursing, nutrition, dentistry, and pharmacy, and 20 years later. Data obtained in the two phases were compared. Questionnaires about hypertension, diabetes, hypercholesterolemia, family history of early-onset cardiovascular disease, smoking, alcohol consumption, and sedentary lifestyle were administered. Cholesterol, blood glucose, blood pressure, weight, height, and body mass index (BMI) were measured. Results Of the 281 individuals (62.9 % women; mean age 19.7 years) initially analyzed, 215 (59.07 % women; mean age 39.8 years) were analyzed 20 years later. An increase in mean values of systolic (111.6 vs 118.7 mmHg- p < 0.001) and diastolic blood pressure (71vs 77.1 mmHg - p < 0.001), cholesterol (150.1 vs 182.4 mg/dL - p < 0.001), blood glucose (74.3 vs 81.4 mg/dL - p < 0.001) and BMI (20.7 vs 23.7 kg/m2 - p = 0.017) was observed. Despite the decrease of sedentarism (50.2 vs 38.1 % - p = 0.015), the prevalence of hypertension (4.6 vs 18.6 % - p < 0.001), excessive weight (8.2 vs 32.1 % - p < 0.001), hypercholesterolemia (7.8 vs 24.2 % - p < 0.001), and alcohol consumption (32.7 vs 34.9 % - p = 0.037) increased. There was no change in the prevalence of smoking. Conclusion Health professionals presented an increase in systolic and diastolic blood pressure, blood glucose, body mass index, and cholesterol over the 20-year study period. Regarding the prevalence of cardiovascular risk factors, increased blood pressure, overweight, hypercholesterolemia and alcohol consumption, and a decrease in sedentary lifestyle were observed.|
|Note:||Reproducció del document publicat a: http://dx.doi.org/10.1186/s12889-015-2477-8|
|It is part of:||BMC Public Health, 2015, vol. 15, num. 1111|
|Appears in Collections:||Articles publicats en revistes (Medicina)|
This item is licensed under a Creative Commons License


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Wartune Events 24 NOV 2018 (Euda Upgrade Refine Cycle)
Hey everyone, in this article I share the
. Wartune Events for the 24th of November 2018 (article + video)(Euda Upgrade Refine Cycle) Article Content:
Special notes and/or new type of Wartune events or exchanges (if any)
Link to REFERENCE article for exact configuration
“Normal” events’ summary in bullet points
Exchanges summary in bullet points (if any)
Summary of balen events
New mount or clothing (if any)
Video of events (link YouTube)
Extra login gift: Daru x10000000, Gold x10000000, Essence of Zeus x5, Energy Stone x1, Clothing Refinement Stone x10 Free event chest in Shop:
RAVE Event: is not running
This event cycle offers chance to upgrade/refine Eudaemons Event Chest / Event Items
Main event item:
Thank-you Envelope Mini event item:
Yarn Ball Reference to Events Config
If you want to see exact configuration of events please check the video or refer to my page:
Wartune Events Configuration REFERENCE Events & Exchanges Summary
Eudaemon Upgrade – 3 days: Use up to 1000 Blood of Zeus per day for rewards.
(event missing?) Eudaemon Upgrade / Blood of Zeus – 3 days: One time rewards for achieving of Eudaemon. level 5 to 10
Eudaemon Diamond Synthesis – 3 days: Synthesize diamonds to receive rewards. to Level 6 . Level 12
Eudaemon Skill usage and upgrade – 3 days: Upgrade Eudaemon General and/or Active skills to receive rewards. Skill . level 3 to level 8
Eudaemon Equipment Refining – 3 days: Refine Eudaemons’ equipment to receive rewards.
Eudaemon Equipment Advancement – 3 days: Upgrade Eudaemons’ equipment to receive rewards.
Soul Engraving – 3 days: Engulf Soul Crystal and Soul Seals to receive rewards. Exchanges
Was expecting a couple of exchanges – seem to be missing. Semi-casher events
Patron’s Gift (1 day) – event that tries to motivate people to spend cash starting from 1 balen reward, then 10 balen, 100, 1000
Big Spender (1 day) – extra rewards for spending 1000-15000 balens; various items
Epic Spender (3 days) – 2000 to 30000 balen spending various rewards
Casher Clothing Exchange (3 days) with the set Conflagrate YouTube Video Link:
Matched Links from DolyGames Sites / Google
NOTICE TO SUPPORTERS
Thank you to those Wartune players who are supporting my work, COSMOS Wartune blog and DolyGames gaming portal & the games that I create for people to play for free.
My goal is to reach 300 PATRON SUPPORTERS to help pay my monthly costs. Currently I have 29 Patrons! (Also thanks to Patrons who supported in the past!)
So please consider supporting my work via
Patreon and check back for new articles and updates at the COSMOS Wartune blog and/or follow announcements on the Twitter page or Facebook page.

domain: hobby


text:
Jameela Jamil and Jonathan Van Ness are two peas in a pod, at least when it comes to this gorgeous pink Rochas gown. The Good Place actress and Queer Eye star each picked the dress for separate industry
The trailer for the upcoming third season of Big Mouth—the show that tackles puberty, and out-of-control sex drives and changing bodies that accompany that—has arrived, and it's absolutely filthy. The
Big Mouth is back and filthier than ever in the first trailer for the third season of Netflix’s hormone-fueled series from the warped minds of creators Nick Kroll, Andrew Goldberg, Jennifer Flackett, Mark
Not only was Netflix’s Big Mouth renewed for a third season not long after its second debuted last fall, but Nick Kroll and Andrew Goldberg’s surreal animated take on the more wild and frenzied aspects
Karamo Brown has had a wild few weeks, ever since it was announced that he would be joining the celebrity cast of Season 28 of Dancing with the Stars. In his latest Instagram post, he can be seen chilling
Here's what you might've missed! 1. Miley Cyrus, Ariana Grande, and Lana Del Rey dropped their new music video for the new Charlie's Angels movie. 2. Chris and Liam Hemsworth found a hidden gem in Australia.

domain: celebrity


text:
How a Graham Cooks lecture, a good knowledge of SPME technology, and the hunt for better direct sample introduction into mass spectrometry led us to a simple solution with surprising performance: coated blade spray.
German Augusto Gómez-Ríos |
There is a demand for analytical tools that can allow scientists, medical doctors, and regulatory agencies to rapidly diagnose, prognosticate, or monitor a system under investigation. However, most of the “traditional” workflows for analyzing pesticides in food and environmental matrices, xenobiotics in biofluids, and metabolites in tissue samples (among others) are slow, burdensome, and difficult to operate. Therefore, analytical tools intended for contemporary problems must not only be rapid, but simple, cost-effective, deployable, capable of providing data in real-time (or close to it) and, preferably, allow for high-throughput analysis. Furthermore, these technologies must be sensitive and selective, and be easily coupled to state-of-the-art detection systems, such as mass spectrometers – all while guaranteeing minimal contamination and long-term operation. Finally, these tools must produce reliable results and, if possible, allow for multiple substances to be monitored simultaneously.
For nearly 30 years, Janusz Pawliszyn and his research group at the University of Waterloo have been developing analytical technologies that can facilitate the rapid and “green” determination of target compounds in complex matrices, particularly in the field of sample preparation. Although his group’s most widely-known contribution came in 1989 with a solid-phase microextraction (SPME) fiber for gas chromatography (1), they have also developed a number of tools over the past two decades that, although less widely acclaimed, deserve equal or greater attention.
To ensure an adequate understanding of recent SPME developments, a quick review of this fascinating technology is in order. Simply put, an SPME device consists of a solid support with a portion coated with a solid or liquid polymer phase (see Figure 1). Contrary to popular belief, the solid substrate can be made of any material (including plastic, metal, metal alloys, paper, or wood) and can embrace diverse geometrical configurations (for example, wires, meshes, pins, rectangular blades, or membranes) to facilitate target applications (2). Though SPME was originally intended for equilibrium-based extractions of volatile and semi-volatile compounds present in the headspace of liquid/solid samples enclosed in a vial/container, continuous technological improvements have expanded its applicability to the direct extraction of target analytes from complex liquid and solid matrices, such as biofluids and tissue. As a matter of fact, improved (thinner and more homogenous) matrix-compatible coatings coupled with state-of-the-art mass spectrometry have made the use of pre-equilibrium SPME increasingly relevant, which has in turn enabled shorter extractions with adequate limits of detection.
Certainly, matrix-compatible coatings have played the most important role in allowing SPME to be used for otherwise extremely challenging applications, as they help prevent matrix components from accumulating on the coating surface; matrix component build up can result in significant matrix effects or poor reproducibility, despite providing adequate analyte recoveries (3). In addition, the use of SPME devices with large surface areas (for example, the rectangular blade, see Figure 1) results in increased extraction rates, which allows lower limits of quantitation while maintaining rapid analysis. Furthermore, such SPME devices can be equipped with a holder that can accommodate 96 blades, which further reduces the analysis time required for each sample (4).
Notwithstanding the exceptional advances in SPME technology, their interface with sensitivity and selective detectors, such as mass spectrometers, has been traditionally performed via chromatographic separations. Consequently, analytical results are generated at a speed that is strictly dependent on the length of the chromatographic run, which limited the technology’s applicability to rapid and on-site screening. It is therefore not a surprise that one of the most lauded features of SPME technology is its ability to be directly interfaced to mass spectrometry (MS) instrumentation (5).
Although multiple SPME-MS couplings have been developed over the last 20 years, one in particular – coated blade spray (CBS) – has taken full advantage of the most recent advances in SPME technology to maintain the traditional operational simplicity of SPME while providing outstanding quantitation performance and speed of analysis (6). Here, I recapitulate my experiences under the supervision of Janusz Pawliszyn in developing CBS, as well as its suitability as a tool for solving some of the aforementioned challenges.
The story begins in 2013, when Pawliszyn assigned me to work on developing a rapid-diagnosis tool based on the direct interface of SPME to MS. Earlier that year, I had read about attempts to directly generate ions from SPME fibers by Pawliszyn (7)(8) and Jentaie Shiea (9). A few weeks later, while attending Pittcon 2013 in Philadelphia, I listened to a talk by Graham Cooks about two substrate spray technologies that were suitable for collecting small amounts of complex matrices (for example, blood and tissue). As Cooks explained, these technologies required no modification to the substrate and could perform electrospray ionization from one of their vertices with the assistance of a solvent (10). Thinking over this presentation – and aware of contributions by other outstanding researchers (7)(9)(11)(12) – it occurred to me that the substrate-spray concept (also described by Nobel Laureate John Fenn in one his patents in 1998, ) could potentially be coupled with the microextraction devices developed by the Pawliszyn group, particularly the blade geometry with a matrix-compatible coating. I was interested to find out if combining these technologies could produce something suitable for the rapid, clean, sensitive, and effortless determination of target analytes in complex matrices using MS.
Anatomically, CBS is a sword-like device made of stainless steel that is partially coated with a matrix-compatible extraction phase (see Figure 1). The extraction phase can either be a polymer or polymeric particles (for example, hydrophilic–lipophilic-balance or octadecyl silica particles) that have been affixed to the substrate surface using a biocompatible binding agent. Analytically, the CBS workflow typically consists of three steps. First, the analytes are rapidly enriched on the coating (≤ 5 min), either by spotting the sample onto the CBS, or by extraction from a vial/well containing the sample. Second, the CBS is rapidly rinsed with water to remove any matrix components that may have adhered onto the coated surface during the extraction process. Finally, instrumental analysis is performed by applying a few µL of an elution/electrospray solution onto the coated area of the CBS. After few seconds (~ 20s), a high voltage is applied to the non-coated area of the blade, which results in the generation of ESI from its tip. The ESI process generates a transient signal that can be used for qualitative or quantitative purposes; the duration of the signal can be as short as 1s, but it can be increased to as much as a few minutes, depending on the application needs (for instance, when performing multiple MS events).
Beyond the solution
Ideas rarely develop as quickly or easily as we would like, and my attempts to develop CBS were no exception. In 2013, our group had limited experience with ambient ionization and modifying MS systems, and this posed a significant challenge. Therefore, in the months following Pittcon, I worked tirelessly on the first CBS prototypes. I was eventually able to construct an MS-interface that was both suitable for this technology and safe for our instruments, and the resultant experimental data was encouraging enough to make it an idea worth pursuing. These early experiments demonstrated that achieving electrospray from a flat surface, such as a blade, was simpler than it was from a fiber, as was originally proposed in 2005. The metal substrate was able to produce stable electrospray by generating a constant electric field, and the thin matrix-compatible coatings facilitated a clean extraction mixture that was both rapidly desorbed into the electrospray solvent and efficiently introduced into the mass spectrometer. One year after the idea was conceived, our first results were published in Angewandte Chemie (6). In that manuscript, we demonstrated that CBS was capable of quantifying sub-ppb levels of cocaine and diazepam in urine and plasma without using chromatography or any other instrumentation aside from the MS system and the CBS. Furthermore, the analyte enrichment step could be performed in less than 1 minute, with a total analysis time of less than 3 minutes. Certainly, these features were revolutionary, especially for the most assiduous SPME skeptics who frequently pointed to its “long extraction times” and the “higher limits of detection” when compared with traditional SPE. Moreover, our results attracted the attention of Bradley Hart from Thermo Fisher Scientific, who has since been one of CBS’s biggest supporters.
Despite our initial successes, we soon realized that there were several features that needed to be improved if CBS was ever to achieve commercial success. For instance, the first blades were coated using a spray mechanism that was both tedious and wasteful. Furthermore, the original blades were handmade, which made it difficult to reproduce the process. Fortunately, while my colleagues at the University of Waterloo and I were assessing novel methods for manufacturing CBS devices, we discovered a methodology that would allow us to homogeneously coat surfaces with a thin layer of particles (Ø≤ 15 µm). The introduction of thin, reproducible coatings enhanced the performance of CBS and speed of analysis, as it ensured better inter-device reproducibility, faster extraction, enrichment, and desorption of the target analytes; and minimal matrix component adherence on the coated surfaces. These improvements were essential in putting us back on the road towards successfully introducing our technology. We’re grateful to Mike Morris from Waters Corporation for providing 5µm particles, which facilitated very thin and robust coatings.
Predictably, criticism of our CBS technology emerged shortly after our initial results were published, with some authors quick to claim that it could not be applied to the analysis of spot-sized sample volumes. To refute this, we demonstrated that CBS could rapidly quantify a drug with a narrow therapeutic range (amitriptyline) from blood droplets (≤ 10 µL) (13). Furthermore, in a subsequent publication, we also revealed that the results obtained by either methodology (spot analysis or extraction from a vial) were comparable, and that the total instrumental time could be reduced to just a few seconds without compromising the technique’s quantitation capabilities (14).
Aware of the need for speed, accuracy, and unmanned operations in clinical laboratories, we demonstrated that, like the traditional blade geometry, 96-CBS devices could be arranged in a holder to perform high-throughput automated extractions from a plate containing an equal number of samples, thus reducing the sample preparation time to less than 7 seconds per sample (15). Furthermore, we showed that CBS was suitable for the rapid screening and quantitation of at least 18 substances with different physicochemical properties at concentrations typically monitored by international regulatory agencies (for example, the World Anti-Doping Agency) (15). Although the total instrumental time in this study was in the order of 20 seconds per sample (MS-time), it can be further reduced, allowing for total analysis times that are faster than any available on-line SPE technology. Moreover, given its structure, CBS can be used to perform analyte enrichment from diverse biological and food matrices without having to worry about cartridge clogging, carryover, or costly repairs in pumping systems. At present, we are focusing on achieving the complete automation of the CBS workflow. Such a system would potentially allow CBS to attain total analysis times of under 10 seconds per sample when monitoring 20 to 30 target compounds – even faster analysis can be achieved for a smaller number of targets or when performing molecular profiling studies.
Since its inception, we have held a firm belief that CBS will be a paradigm-shifting technique not only in the clinical field, but also in the fields of environmental, forensic, and food analysis (16)(17). Its potential applications range from determining pesticides or chemical warfare agents in drinking water, to classifying food samples from different farming origins, to quantitating biomarkers on a droplet of plasma, to name just a few. We envisage CBS as an analytical tool that can radically alter how we go about direct sample introduction to MS. Yet, in the words of AC/DC, “It’s a long way to the top!” and CBS is still working towards widespread acceptance by the scientific community and the industry. The good news is that the US Patent and Trademark Office has recently granted a patent for CBS (18), and we are now teaming up with Restek, one of the world’s leading analytical science corporations, to bring this technology to interested users around the globe. Indeed, JP scientific, the company holding the patent rights for CBS, is open to partnering with any MS companies potentially interested on this technology. It is important to keep in mind that, whereas CBS is intended to be a tool for rapid screening and quantitation, thin-film coated blades without a sharp tip can be used for traditional confirmation experiments via LC-MS/MS; as such, there is ample opportunity for interested scientists to evaluate the performance of this technology using a more traditional determination format. We are eagerly looking forward to hearing about the various ways researchers apply this blade technology to overcome their challenges.
German Augusto Gómez-Ríos is now a postdoctoral fellow in the Pawliszyn lab, Department of Chemistry, University of Waterloo, Canada.
- RP Belardi, JB Pawliszyn, Water Pollut Res J Can, 24, 179–191 (1989).
- ÉA Souza-Silva et al., TrAC Trends Anal Chem, 71, 224–235 (2015).
- EA Souza-Silva et al., TrAC Trends Anal Chem, 71, 249–264 (2015).
- D Vuckovic et al., Anal Chem, 80, 6870–6880 (2008).
- L Fang et al., TrAC Trends Anal Chem, 85, 61–72 (2016).
- GA Gómez-Ríos, J Pawliszyn, Angew Chemie, 53, 14503–14507 (2014).
- J Pawliszyn, US Patent 7384794 (2003).
- M Walles et al., Chromatogr A, 1067, 197–205 (2005).
- CP Kuo, J Shiea, Anal Chem, 71, 4413–4417 (1999).
- CR Ferreira et al., Clin. Chem, 62, 99–110 (2015).
- J Fenn, US Patent 6297499B1 (1998).
- F-L Hsu et al., Anal Chem, 75, 2493–2498 (2003).
- H Piri-Moghadam et al., Angew Chemie, 55, 7510–7514 (2016).
- M Tascon et al., J Pharm Biomed Anal, 10.1016/j.jpba.2017.03.009 (2017).
- M Tascon et al., J Anal Chem, 89, 8421–8428 (2017).
- J Poole et al., Environ Sci Technol, 10.1021/acs.est.7b03867 (2017).
- GA Gómez-Ríos et al., Anal Chim Acta, in press (2017).
- J Pawliszyn, GA Gómez-Ríos. US Patent 9733234 (2015).

domain: chemistry


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Reality check: Sexually transmitted infections vs. H1N1 flu | Amy Johnson
It’s back to school time, which comes with reminders to wash our hands, sneeze into our elbows and prevent the spread of swine flu. All this got me to thinking: What if we put an equal effort into the public health crisis of sexually transmitted diseases as we do to prevent H1N1?
To be fair, I have to admit this is not a totally original idea, but a spin on one introduced by Meg Stone in 2009 in her article “Swine and Dandy,” in which she focused on sexual assault prevention. I decide to take a new twist and talk about sexually transmitted infections.
You might think I’m nuts. After all, the flu is a much bigger problem than STIs, right? Consider this. According to the Center for Disease Control, there were approximately 60 million total cases of H1N1 last year. Some of these statistics are extrapolated due to the lack of people who actually go to the doctor to get tested when they get sick with the flu. Still, they were generous in their calculations.
Contrast that to a wimpy 3.2 million teen girls who have STIs. That’s not total STI cases — that’s just one in four teen girls with STIs. Infections that can cause infertility, cancer and death. The total number of people in America who’ve been diagnosed with an STI is more like 39,487,025 (compiled from 2008 CDC data). Sounds like a public health crisis to me. We better get on this!
What if we equaled the effort that has increased hand sanitizers and education posters about the flu in public restrooms? What if we had condom dispensers in the same proportion, along with posters about preventing STIs in public restrooms (or even in the Department of Licensing, where I was able to sanitize my hands while waiting for my number last week)?
What if the shame and stigma of sexually transmitted diseases disappeared enough that people weren’t afraid to get tested? And more than half the doctors actually brought the subject up with teens during annual check-ups?
What if that shame and stigma about contracting an STI was replaced with a redoubled effort to educate the masses about prevention? If every middle school, high school and college student had access to the information they need to make healthy choices? And what if they also had reminders of safe practices every time they used a public restroom?
Let’s do a reality check, shall we? This won’t happen because too many people in our country don’t want to call attention to sexually transmitted diseases because they still have an underlying attitude that somehow, if a person gets HIV (or syphilis or gonorrhea or chlamydia), it’s their own dang fault.
Yet, we slash health budgets in schools and come out in droves when a district wants to expand its sexuality education to give teens the information they need to stay safe — because that might mean we have to admit that, like generations of adolescents before them, some of them are actually having sex.
Giving information about safer sex practices doesn’t condone the behavior, any more than giving information about health food choices condones a daily diet of junk food. We all know that lots of teens eat pizza and fries and drink energy drinks, but we don’t get embarrassed to talk to them about ways they can be healthier. We don’t keep nutritional information from them about what is healthy so they can make better choices. We shouldn’t withhold information about healthier and safer sexual choices from our youth, either.
Oh, and don’t forget to sneeze into your elbow.


Bangladesh government has taken several measures to upgrade the silk industry
The lives and earning of many people are linked with the historical industry of silk. With the influence of open market, foreign silk is dominating the globe but in spite of that, local silk and cloth made of this silk are quite valued. By investing less amount of money in mulberry plants and silkworms, one can take an initiative to produce silk and there are many scopes to gain self-dependency by that. With the multifaceted initiatives taken by the government, silk cultivation or sericulture is now spreading nationwide.
The Silk Production, Research and Training Centre of Bangladesh Silk Development Board located in Maynamati of Cumilla and controlled by the Textile Ministry has got breakthrough in sericulture. Gradually this center is going towards a profitable stage. Through this center, the government is earning revenue and alongside thousands of people can dream of self-dependency through the training.
According to the authorities, only the uneducated or less-educated women of the rural and urban areas are given hand-to-hand training for mulberry cultivation and silkworm. Due to the efforts of the current government, this potential sericulture is advancing towards prosperity not only in Cumilla but also in other places of the country as well. This sericulture is increasing rapidly in Rangamati and the Hill Tracts.
The Process of preparing silk cocoon from mulberry leaves
Eggs those size is like a dot, and from those eggs come silkworms. From a single silk moth, 400/500 eggs are being collected. However, previously only 20/25 kilograms of silk were produced from 100 eggs here, but now they can yield 50-55 kilograms of silk cocoons.
Species and production
Among the species of silk or mulberry plants produced in Maynamati, there are BM-3, BM-4, BM-6, BM-10, and BM-11 as well as other advanced local and Thai species of seedlings. The leaves of this tree provide food for the silkworms.
On the other hand, there are 25 advanced species of silkworms in this center, which are crossbred among the males and females to breed developed species of silkworm eggs and larvae. Among the 25 advanced species of silkworms, 14 are of pure species, 6 are local, 8 are foreign and 9 are hybrids. It takes three weeks to produce a whole cocoon from the start.
Higher profits in less capital
By investing less capital in sericulture there are lots of scopes for becoming self-dependent by taking self-initiative with mulberry plants and silkworms. Authorities have said that at the Silk Production and Research Centre of Cumilla’s Maynamati, they emphasize on the research of producing matriarchal and patriarchal species of silk.
The Assistant Director of the center Mr. Kanchan Baran Das has informed that after hatching one egg, 400 silkworm larvae are produced and one hundred pieces of such eggs cost 205 Taka only, from which 40 thousand silkworm larvae can be produced.
From one hundred eggs and later silkworms, 30/45 kilograms of cocoon can be made. Each kilogram of cocoon can be sold for 300 taka.
He said that to produce high-quality sari 2 kilograms of the cocoon is sufficient. The silkworms from here are sent to various industrial units especially in places like Rajshahi, Jhineidah, Thakurgaon, and NGOs like IIRD, BRAC, and Karitas etc according to their demand.
In Maynamati, there are three rooms for nurturing silkworms, one factory for producing silk thread, one laboratory, one room for storing various tools and instruments, one dormitory and a facility with 40 beds for the lodging of trainees.
In the last fiscal year, Maynamati Silk Centre produced around 10 thousands of eggs; each hundred eggs are sold to the hill tracts for 205 taka only. It has come to be known that in the beginning of the current fiscal year, 4 thousand eggs have been produced in the last three months, and during the remainder 9 months, it is targeted to produce 12/15 thousand eggs of estimation. Meanwhile, in the last fiscal year after producing 25 thousand mulberry plant seedlings 10 thousand were sent to Khagrachari district, 10 thousand to Feni and 5 thousand to Langolkot and the southern town of Cumilla Each seedling cost was 50 paisa only.
Required more steps to advance the sector
Currently, the Silk Development Board is managing the silk production, training and research centers located in various districts including Maynamati of Burichang Upazilla of Cumilla, Jhineidah, Rajshahi, Thakurgaon, Feni, Bandarban, Khagrachari, and Rangamati.
Industry experts informed that many steps have been taken to increase the import duty of foreign thread and textile to save the country’s silk industry. However, most of the silk farmers are terminal and landless people. They don’t have the sufficient capital. Organizational assistance is also inadequate.
Multiple steps are being taken to eradicate the hindrances in the marketing of their produced silk and cocoons. According to a silk businessman, in the last two fiscal years at least 1.5 million mulberry seedlings have been produced and distributed. This number has been increased to 2 million now.
At present more than 400 tons of silk is produced each year in an average. Along with that almost 50 tons of silk thread is also produced. However, the industrial units of silk mills in Rajshahi and Thakurgaon require a greater amount of silk thread.
In many districts, sericulture has been included in the government’s action plan of ‘A Home, A Farm’. Under this, currently, thousands of farmers are cultivating the mulberry plant in 95 Upazillas of 35 districts. This number is increasing gradually.
The Maynamati Silk Production, Research and Training Center of Cumilla have been successful in sericulture. The production operation is run every day by 10 working heads among whom only 3 persons are from the government and 8-10 men and women laborers hired according to the amount of task at hand. There are training facilities as well throughout the year.
The Assistant Director of Maynamati Silk Center, Kanchan Baran Das said that in spite of the inadequacy of workforce and allocated budget this silk center is in a profitable state in producing eggs, cocoons, and seedlings through production and research.
“People who are interested in sericulture even at their own home we will provide all sorts of assistance, he said.
There is a big demand for silk threads in the country but the foreign threads and fake silk thread, which are dominating the market, are a big hindrance to bringing this industry to a good shape, he opined.


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WARNING: This product is for research use only, not for human or veterinary use.
MedKoo CAT#: 406302
Description: FTI-2153 is a potent farnesyltransferase inhibitor. FTI-2153 inhibits bipolar spindle formation during mitosis independently of transformation and Ras and p53 mutation status. FTI-2153 was reported to block bipolar spindle formation and chromosome alignment and causes prometaphase accumulation during mitosis of human lung cancer cells.
MedKoo Cat#: 406302
Chemical Formula: C25H30N4O3S
Exact Mass: 466.20386
Molecular Weight: 466.6
Elemental Analysis: C, 64.35; H, 6.48; N, 12.01; O, 10.29; S, 6.87
FTI-2153 is not in stock, but may be available through custom synthesis. For cost-effective reason, minimum 1 gram order is requested. The product will be characterized by NMR, HPLC and MS analysis. Purity (HPLC) is usually >98%. CoA, QC data, MSDS will be provided when product is successfully made. The estimated lead time is 2-3 months. Please send email to email@example.com to inquire quote.
Synonym: FTI2153; FTI 2153; FTI-2153
IUPAC/Chemical Name: L-Methionine,N-[[5-[[(1H-imidazol-4-ylmethyl)amino]methyl]-2'-methyl[1,1'-biphenyl]-2-yl]carbonyl]-, methyl ester
InChi Key: KZCMMZOLCBZLNG-QHCPKHFHSA-N
InChi Code: InChI=1S/C25H30N4O3S/c1-17-6-4-5-7-20(17)22-12-18(13-26-14-19-15-27-16-28-19)8-9-21(22)24(30)29-23(10-11-33-3)25(31)32-2/h4-9,12,15-16,23,26H,10-11,13-14H2,1-3H3,(H,27,28)(H,29,30)/t23-/m0/s1
SMILES Code: CSCC[C@@H](C(OC)=O)NC(C1=CC=C(CNCC2=CNC=N2)C=C1C3=CC=CC=C3C)=O
The following data is based on the product molecular weight 466.6 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
|Concentration / Solvent Volume / Mass||1 mg||5 mg||10 mg|
|1 mM||1.15 mL||5.76 mL||11.51 mL|
|5 mM||0.23 mL||1.15 mL||2.3 mL|
|10 mM||0.12 mL||0.58 mL||1.15 mL|
|50 mM||0.02 mL||0.12 mL||0.23 mL|
1: Crespo NC, Delarue F, Ohkanda J, Carrico D, Hamilton AD, Sebti SM. The farnesyltransferase inhibitor, FTI-2153, inhibits bipolar spindle formation during mitosis independently of transformation and Ras and p53 mutation status. Cell Death Differ. 2002 Jul;9(7):702-9. PubMed PMID: 12058275.
2: Crespo NC, Ohkanda J, Yen TJ, Hamilton AD, Sebti SM. The farnesyltransferase inhibitor, FTI-2153, blocks bipolar spindle formation and chromosome alignment and causes prometaphase accumulation during mitosis of human lung cancer cells. J Biol Chem. 2001 May 11;276(19):16161-7. Epub 2001 Jan 11. PubMed PMID: 11154688.