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Posted on Wednesday, December 31st, 2014 by Angie Han
We present to you the very last Sequel Bits of 2014. After the jump:
- Woman in Black 2 unveils a motion comic prequel
- See a leaked image of the Jurassic World Lego set
- Bryan Mills has a big problem in this Taken 3 clip
- Katherine McNamara joins The Maze Runner 2
- Could Randy Quaid return for Independence Day 2?
- Jamie Oliver is still sad he wasn’t in The Hobbit
- Watch the latest Punk’s Dead: SLC Punk! 2 trailer
- Tom Hardy glowers in a new look at Mad Max: Fury Road
Read More »
It’s a crazy, mixed up world and we are thankful for movies, excluding Valentine’s Day starring every safe, boring white actor ever, that offer proof. Slashfilm’s Weekend Weirdness examines such flicks, whether in the form of a new trailer for a provocative indie, a mini review or an interview.
In 1986, a supernatural moto-fantasy about a murdered bro who returns via a phantasmic, black stealth race car to kill his killers was released on Earth and no one gave a shit. More than two decades later, The Wraith, though forever without a wet ‘stache lick from Peter Travers, is cult-minted for being memorable-enough ’80s-ploitation. Next month sees the release of a Special Edition DVD that adequately recognizes and explores the movie’s legacy and history with commentary courtesy director Mike Marvin and featurettes on the film’s semi-iconic Dodge racer and co-star Clint Howard (who, if not semi-iconic himself, sported a semi-iconic wig inspired by Eraserhead for the film).
Revisiting The Wraith, what’s interesting is how this derivative hybrid of genres and classic revenge films—Marvin references High Plains Drifter and The Road Warrior—remains sublimely adolescent but in an inherently cold and detached way. Stranger still is how this suits the film’s undead hero, vehicle, and hints of an afterlife with a decidedly mechanical bent. And before viewing the S.E. I had no idea a crew member died and many others were injured in a chase scene gone awry. One stunt coordinator recounts how a grip fell 60-feet down a rocky embankment and was only found knocked-out but okay hours later. Nor did I know (or need to) that a sunbathing scene with lead star Charlie Sheen as the titular, ghostly hero and co-star Sherilyn Fenn (Twin Peaks, Wild at Heart) was shot on a “near-freezing” day. Hearing these stories, I wonder now if the troubles of the production didn’t contribute to the overall tone. And looking back at the film itself, which was released the same year as Top Gun, Ferris Bueller, and Blue Velvet, might The Wraith, however unintentionally, deserve to be called Lynchian?
Read More »


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The Delhi government on Tuesday announced that it will provide 450 types of medical tests free of cost at its hospitals and health centres from January 1.
The Delhi government has been providing 212 medical tests free of cost till now.
Click Here: FIJI Rugby Shop
These include haemoglobin test, stool routine, kidney function test, liver function test, lipid profile, blood glucose random test, etc.
In addition to this, some radiology tests have also been made available for free to Delhi residents across all income groups since 2017.
The free service includes MRIs, CT and PET scans, and ultrasounds.
On Tuesday, Delhi Chief Minister Kejriwal gave the nod to a health department proposal for making 238 more tests free of cost at hospitals and Mohalla Clinics.
The facility will be available to the people of Delhi.
Chief Minister Arvind Kejriwal said the move will help those unable to afford private healthcare.
Kejriwal said that providing good quality health and education to all, irrespective of their economic status, is his government’s mission.
“Providing good quality health and education to all, irrespective of anyone’s economic status, is our mission. Healthcare has become very expensive. Many people cannot afford private healthcare. This step will help all such people,” he tweeted.
Providing good quality health and education to all, irrespective of anyone’s economic status, is our mission. Healthcare has become v expensive. Many people can’t afford pvt healthcare. This step will help all such people https://t.co/2B94b6YPCZ
— Arvind Kejriwal (@ArvindKejriwal) December 13, 2022
The AAP government launched Aam Aadmi Mohalla Clinics, also known as Mohalla Clinics, in 2015 as primary health care centres.
It has been one of the flagship programmes of the AAP government.
On average, one mohalla clinic handles 116 patients daily; together, 520 of these facilities have over 60,000 patients daily.
Last month, Arvind Kejriwal announced that 100 special mohalla clinics for women will be opened in the national capital. He added that an all-women staff will run these.
Children below 12 years and women will get free of cost treatment at these facilities, he said.
For more on news and current affairs from around the world please visit Indiatimes News.

domain: medical


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Not Accepting new patients
Offers My Health Online
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Monday, 8:30 a.m. to 5:30 p.m.
Wednesday, 8:30 a.m. to 5:30 p.m.
Thursday, 8:15 a.m. to 12:30 p.m.
Education & Training
St. Louis University School of Medicine, St Louis, MO, 1992
Loyola University Medical Center, Maywood, IL 1993
Loyola University Medical Center, Maywood, IL 1995
Dr. Fong has been practicing medicine for over 18 years.
Dr. Fong enjoys traveling, snowboarding, camping, photography and spending time with my family and friends. She was raised in San Francisco and has fun taking her children to San Francisco to be tourists.
This physician directory is provided as a convenience to you. It is not intended as a recommendation, referral, or endorsement of any particular provider. Physicians are not employees or agents of Sutter Health or its affiliates. The information is submitted by each doctor and we make no guarantee or warranty as to the accuracy of the information. Sutter Health is not responsible for any loss or damage caused by your reliance on this information. You should verify the accuracy of the information directly with the physician's office.


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Hand Cast Heart Necklace in Vermeil/Gold-FilledRegular price $100.00
A reminder everyday to love yourself and perfect for gift-giving.
The hand cast vermeil charm (18k over sterling silver) measures .60" in width, and will slip off the chain easily so the chain can be worn alone as a layering piece.
The singapore-style chain is made from 1/20 14k gold-filled, and is water and tarnish resistant
Available in 16" and 18" lengths
Hand-assembled in our studio

domain: design


Authors: Longfeng Wu*,
Topics: Social Geography, China, Spatial Analysis & Modeling
Keywords: Urban Green Space, Socio-spatial Inequality, China Major Cities,
Session Type: Paper
Presentation File: No File Uploaded
Green space, as an important component of urban system, deliver multiple benefits to urban residents. Unfortunately, studies have confirmed that green spaces are not equally accessible among different socio-economic groups in urban areas. Such unequal socio-spatial pattern of accessing green spaces lead to many other undesirable outcomes including social segregation, dislocation, and gentrification, and finally causes exclusive urban development benefiting a smaller portion of population.
Using multiple open source data sets and spatial statistical analyses, this paper examines the spatial pattern of socio-economic groups vis-à-vis the distributional pattern of their access to urban green space at residential community level in Beijing. The results indicate that public green spaces tend to better serve the marginalized groups. Advantaged socio-economic groups are well served by internal vegetations in the gated communities in which they live. The socio-spatial patterns of accessing green spaces in Beijing is a result of the interplay of many factors, from the city’s original geographical characteristics, to the historical layout of the old city, urban planning and land use decisions, afforestation policies and projects, and even the public financial stresses involved in urban greening efforts. The result of this analysis identified locations that might harbor inequalities in the access to public green spaces. Such information allows decision makers and planners to accurately invest in afforestation projects for the purpose of promoting social equality, especially for those groups with lower levels of accessibility to green spaces


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Aloe vera plants are popular household additions, but all of a sudden, your beloved aloe vera plant is beginning to look a little droopy. Its leaves have started to bend, and you want to know why. And most importantly, you want to know how to fix it.
Aloe vera leaves can bend for a number of reasons, the most common of which is a lack of sufficient light. However, bending aloe vera leaves can also be caused by poor watering practices, fungal/bacterial diseases, low temperature, and improper containers, among other factors.
Thankfully, there are feasible solutions to each of these problems, and this article will be your guide to the whys, what’s, and hows of aloe vera maintenance! Wild aloe species are known to live up to 100 years, so don’t panic – your aloe vera plant will be just fine.
Why Are My Aloe Vera Leaves Bending?
In order to know how to fix your bent aloe vera and prevent the leaves from bending in the future, it is important to understand what causes aloe vera leaves to bend in the first place.
One of the most common reasons for aloe vera leaves bending is insufficient light. Aloe vera plants require a lot of sunlight, and although artificial light works fairly well, direct sunlight is ideal as it is best for photosynthesis.
Insufficient light can cause the aloe leaves to weaken and eventually bend (however, it is important to keep in mind that too much direct sunlight can also cause damage, resulting in reddened and bent leaves as the water evaporates).
Poor Watering Practices
Another common reason for aloe vera leaves bending is poor watering practices. Aloe plants do not need much water and only need to be watered once every two weeks (although the watering frequency varies based on the season, in hotter climates, aloe plants will require a bit more water).
It can be easy to overwater your plant, particularly in an attempt to save it, but underwatering an aloe plant is much better than overwatering, which can cause the leaves to wilt and bend and is much more difficult to recover from.
Fungal diseases can cause the leaves of an aloe vera plant to bend. One of the most well-known and destructive fungal diseases that impact aloe plants is dry rot. Dry rot is a fungal disease that dries out the plant from the inside. This causes the leaves to weaken and bend, which is one of the first signs of dry rot disease.
Like other desert plants, aloe vera plants thrive in warm and sunny environments. The ideal temperature for aloe plants is between 64 and 77 degrees Fahrenheit. When exposed to temperatures below 50 degrees Fahrenheit, the leaves of an aloe plant may begin to bend as it becomes susceptible to the cold.
This can be caused by various factors, but commonly it is from exposure to cold air from a window or water that is too cold being used to water the plant.
When it comes to aloe vera plants, having the correct container is incredibly important for their growth and development. While they don’t need overtly huge pots, pots that are too small and/or too shallow can cause an array of problems.
Aloe vera plants require lots of space to grow and for their roots to expand, and containers/pots that are too shallow can cause the plant to become suffocated and cramped, resulting in drooping and bent leaves.
Young aloe vera plants, in particular, are susceptible to bacterial infections because they don’t have the proper immune system that is required to fight them off. One of the first signs of a bacterial infection is often bent leaves, which occur as the plant weakens and can indicate infections such as soft rot disease, which is common among aloe vera plants.
Can You Fix Bending Leaves Of Aloe Vera?
Unfortunately, once the leaves are bent, there is no way to revitalize or unbend them, and therefore it is best to remove them and use them later so they don’t go to waste. However, just because some of the leaves are bent does not mean that the plant is not able to be saved or that you can’t prevent leaves from bending in the future.
Before attempting to revive your aloe plant, you should make sure it can actually be saved. If your plant has bent leaves but is showing signs of improvement, it is definitely salvageable and worth the time and effort! But if the leaves are wilting and turning a darker color, it might be time to part with your plant.
Should I Cut Bent Aloe Leaves?
You can, and should, cut off aloe vera leaves that have bent. It is actually optimal for the health and appearance of the plant.
Once bent, the leaves will not recover and unbend on their own, and this can inhibit the overall growth of the plant. It is also a good idea to remove yellowing or infected leaves because they are permanently damaged and will only hinder the plant’s health and wellbeing.
Removing bent and dying leaves can help to revive the aloe and encourage new growth. It will also allow the plant to return to its original shape, returning it to its former glory and beauty. An added plus is that the leaves can be used for healing properties, so they won’t go to waste if you remove them!
How To Keep Aloe Leaves From Bending
Due to the fact that bent aloe leaves cannot be revived, it is important to take preventative measures to keep your plant healthy and to keep the leaves from bending.
Sufficient lighting is crucial to keeping an aloe vera plant healthy and in good shape. These plants need around six to twelve hours of direct sunlight, but the occasional shade is also important. Leaving your aloe plant on a windowsill or another well-lit area will help to keep it healthy and allow photosynthesis to occur, keeping the leaves from wilting but also giving it some shelter from the sun.
Good Watering Practices
Aloe vera plants need a well-balanced and well-planned amount of water, and it is important to avoid overwatering and underwatering your plant. However, it is best to underwater an aloe plant, as this is easier to revive and recover from, whereas overwatering can cause permanent damage and drown your plant, which can cause the leaves to droop.
Watering frequency varies depending on the season, but once every two weeks is typically sufficient.
Having a proper pot or container for your aloe vera plant is important to prevent the leaves from bending. If the container is too small or too shallow, the roots won’t have enough space, and the plant can become cramped and suffocated, causing the leaves to bend and lose their strength.
Larger pots with proper drainage allow the plants to have enough space to grow without suffocating or becoming overwatered.
If aloe plants become too cold, it can cause the leaves to bend or wilt. To prevent this, it is important to make sure that your plant is kept in a warm area.
Aloe plants are typically well adapted to changes in temperature and can handle temperatures above 78 degrees Fahrenheit. However, they cannot withstand frost, and it is best to keep the temperature of their environment above fifty degrees Fahrenheit.
Aloe vera plants grow best in certain types of soil, which can help encourage healthy plants and therefore keep the leaves from bending.
Soil can be prepared from sand, leafy soil, turf, and humus, and you could also use a soil mixture specifically pre-prepared and packaged for aloe plants. Having loose, drainable soil is optimal for aloe plants, and if you need to further loosen the soil, you can add some charcoal or brick chips.
Aloe vera plants require annual transplanting, and every time it is transplanted, it’s important to move your plant to a bigger pot to encourage continued growth. Once your aloe plant reaches a more mature size, you can repot it once every three or so years or replace the topsoil to keep it healthy, which will help to prevent the leaves from bending.
Fungal and bacterial diseases can cause a significant amount of damage to aloe vera plants, including the bending and wilting of leaves. These diseases and infections can be easily prevented by taking proper care of the plant, following the above tips such as proper light and watering practices, all of which keep the plant healthy and can therefore prevent diseases.
How To Remove Bending Leaves From Aloe Vera Plant
If there are bent leaves on your aloe vera plant, there are a number of ways to remove them in order to keep the rest of the plant healthy, and they are all quick, safe, and easy solutions!
Cut off the Leaves with Shears
When removing aloe vera leaves from a damaged or suffering plant, it is important to use clean shears. Prior to using the shears, it is crucial that they are sterilized with alcohol in order to prevent the plant from getting any kind of infection.
For small to medium plants, a knife is adequate to use to cut off the leaves, while shears are best used for larger aloe plants. It is also important that the blade used is sharp enough to create a clean cut rather than to saw at the plant and potentially create more unnecessary damage.
Cut from the Base
To remove aloe vera leaves from the plant, rather than starting toward the middle of the plant or higher up, you should start by removing the leaves at the base of the plant. It is also important to be careful when cutting leaves off of the aloe plant to avoid harming the stem or other healthy leaves that are important to keep the plant thriving and growing.
Use Rubber Bands
It is easy to accidentally cut off healthy leaves when trying to remove bent ones on an aloe vera plant; however, there are a few ways to avoid this in order to keep the plant as healthy and viable as possible.
A common way to isolate the leaves you want to cut, in this case, the bent or damaged leaves is by using rubber bands to tie the healthy leaves together, facilitating the pruning process to clean up the plant more efficiently.
Bend the Leaf Downward
Similar to the use of rubber bands, bending the leaf you wish you remove down away from the rest of the leaves will help to isolate it. This allows you to safely cut off the desired leaf or leaves and refrain from accidentally removing the healthy ones and weakening the remainder of the plant.
Pull off the Leaves
Although it is often simpler and more efficient to just cut off the aloe leaves, it is also possible to remove bent or damaged leaves by pulling them off. This is most commonly done during the re-potting process, and you can carefully peel off the leaves from the base. Leaves that are already weakened or dead will come off particularly easily.
Aloe vera plants are excellent to grow and take care of; however, under certain conditions, their leaves can begin to bend and droop, indicating that something needs to be changed in order to keep them in good health.
There are several factors that could cause aloe vera leaves to bend, such as insufficient light, poor watering practices, shallow potting and containers with poor drainage, adequate temperature, and diseases or infections.
At the end of the day, each of these problems has feasible solutions, but in order to keep the plant healthy and growing well, it is crucial to remove the bent or dying leaves. Maintaining good care routines for your aloe plant will prevent its leaves from bending in the future.

domain: agronomy


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HEAL - TEACH - SUPPORT
GERI - ANSEYE - SIPOTE
Heal - Teach - Support: These three words define what we do at Hands Up for Haiti. Our mission - to deliver lifesaving health care to the sickest and most impoverished people of northern Haiti - goes beyond the delivery of care: our goals include capacity building of local leaders and programs, support of programming for which local doctors, nurses, community health workers, and support personnel take ownership, and strengthening partnerships with groups that share these goals. We don’t just teach and have our visiting teams deliver care: we train our Haitian counterparts to become teachers and program leaders and coordinators. We believe that this capacity building and teaching is essential to the development of a sustainable health care system in Haiti, one with a positive and dramatic impact. We coordinate our efforts with both the Cap Haitien Health Network and the Ministry of Health in Haiti to provide programs in maternal child health, malnutrition treatment and prevention, preserving and restoring vision, treating chronic illnesses such as diabetes and hypertension, and Mobile Santè, outreach medical clinics to communities in need. These partnerships and joint efforts magnify the impact of our work.
Haitian Run + Haitian Led = Lifesaving Programs that Build a Better Future
HUFH began its work in Haiti in 2010 when a small team of doctors, nurses and other health professionals led by co-founder Dr. Jill Ratner volunteered to contribute their medical knowledge and skills to help heal communities shattered after the devastating earthquake. This first group of medical volunteers was directed to Cap Haitien, located on the northern coast, a city spared much of the earthquake’s destruction but still very much in need of medical care for its residents and the many refugees who fled there, especially the children.
Jill Ratner, MD, cofounder of HUFH
Pre-pandemic, HUFH sent over 100 medical teams to Cap Haitien and the surrounding region, initially focusing our efforts on Cap Haitien and Limonade, a rural area not far from Cap Haitien. Our global health teams frequently worked in the coastal Bas Limbe region, increasing to the west the areas where our volunteers perform outreach and support the community, its doctors, nurses and community health workers.
We quickly realized that in-country, Haitian-led programs were key to achieving our mission and our goals of reducing barriers to care, improving medical compliance, reducing childhood malnutrition (currently 1/5 children), infant and maternal mortality and premature death, and increasing healthy outcomes for the people we serve. Hence, HUFH evolved from a “parachute” organization to one that teaches and supports a Haitian staff who know best how to deliver healing care to their communities.
Today, in the face of ongoing challenges in Haiti, our vision leader the way: Haitian Run & Haitian Led = Lifesaving Programs that Build a Better Future. This vision is the key to a secure path to scalable sustainability. By empowering the people who are disempowered by poverty and by history, we are making change that lasts and ensuring that our work continues. Today HUFH focuses on health care programs delivered by our Haitian staff to their own communities; we support a Haitian staff of more than 50 medical and other professionals, community health workers and lay staff. Our lifesaving programs include:
- Fighting Childhood Malnutrition
- Hypertension Screen and Treat
- Prenatal Care, Education and Nutrition
- Community Water Project
- Community Health Education
- Mobile Santè, outreach health care clinics led by HUFH medical teams in remote areas that have little access to medical care
- Emergency Hospital and Surgical Fund to provide advance care for our patients.
We continue to provide targeted program support, both in person and remote; Medical Education and Specialty Training is conducted for both doctors and nurses. Our Eye Team works with their Haitian counterparts to make high quality eye care available. And we have made a commitment to train the next generation of doctors and nurses in our Global Health Education Program.
We came in response to a disaster.
We stay to build a better future for the children of Haiti.
We succeed with the help of dedicated people like you.
"During our first trips, many of us heard the phrase, 'You can leave Haiti, but Haiti will never leave you.' Many of us who participated in those first missions carried a piece of Haiti in our hearts after we returned home. Out of that inspiration, Hands Up for Haiti was born and even today it is what continues to drive us and help us carry out our mission."
Mary Ann LoFrumento MD, cofounder of HUFH
- Life expectancy is 63 years (World Bank 2013)
- 30% of the population is considered food insecure (World Food Programme 2015)
- Infant mortality: 55 per 1000 births (UNICEF 2015)
- 59 per 1,000 born in Haiti die before reaching their first birthday (Ministry of Health 2012)
- Under five mortality rate: 88 per 1000 live births (Ministry of Health 2012)
- An estimated 1 in 285 births will result in a woman's death, a ratio about 16 times higher than in the United States (Partners In Health 2014)
- About 1500 preventable deaths from cervical cancer yearly, 50 times higher than in the US (GHESKIO Centers 2016)
- Waterborne illnesses, such as typhoid, cholera, and chronic diarrhea, account for more than 50% of deaths in Haiti every year
- More than 500,000 Haitians, about 5% of the population, are legally blind; more than 80,000 have no light perception at all


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|Jmol-3D images||Image 1|
|Molar mass||638.65 g mol−1|
|Except where noted otherwise, data are given for materials in their standard state (at 25 °C (77 °F), 100 kPa)|
|(what is: / ?)|
Evacetrapib is a drug under development by Eli Lilly & Company (investigational name LY2484595) that inhibits cholesterylester transfer protein, which transfers and thereby increases high-density lipoprotein and lowers low-density lipoprotein. It is thought that modifying lipoprotein levels modifies the risk of cardiovascular disease. The first CETP inhibitor, torcetrapib, was unsuccessful because it increased levels of the hormone aldosterone and increased blood pressure, which led to excess cardiac events when it was studied. Evacetrapib does not have the same effect. When studied in a small clinical trial in people with elevated LDL and low HDL, significant improvements were noted in their lipid profile.
Evacetrapib is one of two CETP inhibitors currently being evaluated (the other being anacetrapib). Two other CETP inhibitors (torcetrapib and dalcetrapib) were discontinued during trials due to increased deaths and little identifiable cardiovascular benefit (despite substantial increases in HDL). Some hypothesize that CETP inhibitors may still be useful in the treatment of dyslipidemia, though significant caution is warranted.
In a 2014 study in 165 Japanese patients Evacetrapib decreased CETP activity alone or in combination with atorvastatin. Phase III trials are running with completion expected in 2016. ACCELERATE is studying evacetrapib in participants with high-risk vascular disease. ACCENTUATE is studying patients with hyperlipidemia or diabetes.
- Cao G, Beyer TP, Zhang Y, et al. (December 2011). "Evacetrapib is a novel, potent, and selective inhibitor of cholesteryl ester transfer protein that elevates HDL cholesterol without inducing aldosterone or increasing blood pressure". J. Lipid Res. 52 (12): 2169–76. doi:10.1194/jlr.M018069. PMC 3220285. PMID 21957197.
- Joy T, Hegele RA (July 2009). "The end of the road for CETP inhibitors after torcetrapib?". Curr. Opin. Cardiol. 24 (4): 364–71. doi:10.1097/HCO.0b013e32832ac166. PMID 19522058.
- Nicholls SJ, Brewer HB, Kastelein JJ, Krueger KA, Wang MD, Shao M, Hu B, McErlean E, Nissen SE (2011). "Effects of the CETP inhibitor evacetrapib administered as monotherapy or in combination with statins on HDL and LDL cholesterol". JAMA 306 (19): 2099–109. doi:10.1001/jama.2011.1649.
- "Efficacy, safety, tolerability, and pharmacokinetic profile of evacetrapib administered as monotherapy or in combination with atorvastatin in Japanese patients with dyslipidemia.". Am J Cardiol. Jun 15, 2014. PMID 24786356.
- "A Study of Evacetrapib in High-Risk Vascular Disease (ACCELERATE)".
- "Study of Evacetrapib (LY2484595) in Participants With High Cholesterol (ACCENTUATE)".


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Lawmakers Want Speaker Neutrality Law amid Deadlock
SEOUL, Oct. 2 (Yonhap) -- A revision of the parliament law to clearly stipulate the speaker's duty to remain neutral may emerge as a way to resolve the latest deadlock at the National Assembly, political pundits said Sunday, as the public is growing impatient over prolonged discord that has disrupted normal proceedings.
There has been a rising debate over the political neutrality of National Assembly Speaker Chung Sye-kyun, with the ruling party claiming the speaker's recent actions intentionally gave support to the opposition bloc affected the impartiality of parliamentary affairs.
The deadlock at parliament began when the opposition parties passed a motion asking for the dismissal of a Cabinet minister, despite Saenuri's boycott of the session. Saenuri and the presidential office have argued the dismissal motion was politically motivated and had nothing to do with actual wrongdoing.
The Saenuri, in addition, claimed the vote itself did not follow parliamentary rules, although Chung argued all due procedures were respected.
The two side's relationship further deteriorated when a voice recording obtained by the ruling party showed Chung saying that the Saenuri should not "ask for something for free," indicating the speaker may have wanted to utilize the dismissal motion to force concessions on other issues that are important to the opposition.
Chung is originally from the main opposition Minjoo Party of Korea but left the party in accordance with the South Korean law that the speaker cannot belong to a party.
Observers said the ruling and opposition bloc may seek to reach a compromise by reviewing the so-called "Chung Sye-kyung prevention law," proposed by Saenuri's party whip, which calls for clearly stipulating the political neutrality of the parliamentary speaker.
Existing law only stipulates that the National Assembly speaker "cannot be a member of a party from the second day of designation during the term." Thus, the proposed bill is set to make the political neutrality not a moral value, but a legal responsibility of the speaker.
"While the majority of lawmakers in Saenuri are opposed to making concessions, we are looking into other ways as Chung is unwilling to budge," said Rep. Cho Won-jin of Saenuri, who heads an emergency committee on the issue.
Pundits added although Chung is clashing with the ruling party, the Saenuri is also currently feeling the heat, as its boycott of the parliamentary audit can cost it public support.
Others said that with the speaker showing no signs of resignation, Saenuri does not have the seats to oust Chung from his post, requiring the party to make compromises.
Accordingly, some Saenuri members have said opting for a new parliamentary law will be more beneficial for the party because it can save face while sending a message to the general public that Chung's actions need to be checked to ensure smooth running of the National Assembly.'

domain: news


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helios sun chrysos golden
everlasting, immortelle, strawflower.
calling on circles, time less time.
that tooth and claw may rest a while
among herbaceous and honeyed threads.
deep in ancient caves where shamans once traveled to other worlds the flicker of firelight reveals art meant to be viewed in space and also recently theorized, in time. with the movement of flame over painterly effects including color shifts and superimpositions- herds run, an ibex turns her head, a deer shape shifts into a bull, and hidden animals emerge from brambles.
[image of a roughly 30,000 year old cave painting from Chauvet Pont D'Arc.
theory from "The First Picture Show: Cinematic Aspects of Cave Art" by Edward Wachtel]
animation: the state of being full of life.
projection: a thing that extends outward from something else.
portal: a sacred entry into another world.
motion. picture. magic.
[scene from "Northern Exposure" where Leonard the healer buries Ed's hand in the dirt, a ritual that over the course of time will reveal that Ed has been called to be a Shaman.]
"To concoct, with whatever illegitimate art, a perfume from the poisons themselves. To find in obscure herbs and urges, in the very vulnerability, a trace of remedy- from the stale cadaver the precursor for the elixer."
magic is empowerment by attention to detail.
from Pharmako Gnosis by Dale Pendell
"Mimetic dancing, accompanied by bird wing whistles and pipes, was a prelude to music and poetry. Dancing grew out of observing the movements of animals and implies human identity with them. Dance gave rise to rapture, which led to consciousness, the realization of death, and the hope of rebirth. Out of this, art is born."
from Lady of the Beasts; the Goddess and Her Sacred Animals by Buffie Johnson
"To think of color as a substance with density and propensity to fluctuation and change, like the light flowing through the forest, light purple, some subtle mist of green and blue with some red and yellow in there too- was to realize more than color as in coloring. It was some other medium altogether, a curious light lightness, for which the term polymorphous magical substance seems appropriate. Such a substance helped me get out of the spot-of-color-on-the-page idea of color and pushed me into thinking of the great conjuring tricks of shamans reported the world over, tricks in which a plasma-like sense of the being of being flourishes...
I take the fine meshed connectivity between bodies to be where song and the color spirit take us to and from where they emerge... intelligence working in a subterranean manner, like the signals that operate between the moon and the oceans..."
abridged notes from What Color is the Sacred by Michael Taussig and the words of J.N.B Hewitt
barn projection of The Color of Pomegranates by Sergei Parajanov. Inspired by an Iranian painting of two lovers with the same face, Parajanov chose to have Sofiko Chiaureli play both the part of artist and poet Sayat Nova as well as his love and muse.
The dissolution of boundaries is the communion of the divine work, the color, the polymorphous magical substance, the physical chemical psychological transformation.
burn. dissolve. seperate. combine.
(for the tarot enthusiasts... thoth Key-14 Art is the completion of Key-6 The Lovers also known as the divine twins)
"a state of mystical reverie incepted by vast abscenses." (notes from the library)
"Spirit Meadow is sometimes described as a four sided field bounded by the states of sleeping, waking, dreaming, and trance; it is simultaneously considered a place of embodied power and an actual place... serves as both a state of orientation for the dreamer and also the fecundated locus that gives birth to dreams themselves."
notes from Veneficium by Daniel A. Schulke
detail spirit meadow 2020. weaving. 32"x 88" silk, wool, cotton, linen
the topography of dreamscape, worship, labor.
vivid, scented, sacred.
a tangle of feast and famine.
a source of both delerium and ecstasy.
"a place that is not a place"
a pharmacopeia of possibility.
one thing can be turned into another because at the deepest level they are really the same thing.
hoc est corpus neum. this is my body gnosis. knowledge chrysopoeia. transmutation into gold
the outline as holy gesticulation. (notes from the library)
"What is contemplation? The word derives from the root tem to cut."
(atom, anatomy, diatom, contemplate, temple, tome)
"To what severance, to what separation does the word allude? In order to understand the allusion, we should try to relive an experience half forgotten, something most precious lying hidden beneath the psychic rubbish that modern man heaps upon his heart. if we want to call that experience back to life, we should recall the sense of awe and wonder we feel in certain strangely meaningful places.
... In such places at such moments, a kind of reverence and fear even today finds access to the dulled senses of modern men. But what was such a feeling to a seer, to a priest-astronomer of say, 7000 BC? His fierce imagination was swayed and enthralled by the splendor of the revolving planets and of the constant stars upon the black vault of night. Those same essences that burned in the sky, he perceived when he hit upon some awesome, majestic spot. when in the outline of a rock, or of a tree, or of an animal he detected a luminous quality, full of freshness and grace, a swift delicate rhythm.
he recognized the essence of silvery Venus up in the sky. And thus in all the qualities of earthly things, he perceived the manifestation of some star. In fact the same essence was in turn a star, a mineral, a plant, an animal; especially certain arresting spots in the landscape were hallowed by it. They possessed that power stunningly and gloriously. The seer in such places felt the holy essence beckoning to him. Something from the depths of his being delicately leapt out towards it and communed with it. Our shabby modern language still reminds us of the intensity of those archaic events when it calls certain places "genial" : inhabited by an unworldly being, a genie.
"On such land the seer would feel called, impelled to perform a ritual, the natural response to a nod from above."
from Contemplation and Action in World Religions essay by Elémire Zolla
a phone recorded first draft of a color of a feeling of three walls, three windows and a host of apparitions. numen.
plant of frenzy.
L. temulentum false wheat
the intoxicating twin. a symbol of subversion. a mimic weed.
In his book Bread of Dreams, the Italian scholar Piero Camporesi argued that European peasantry lived in a state of semi-permanent hallucination from bread adulterated with more malign grains, which they may have sought as an escape from daily life.

domain: artistic


Red card appeal turned down at afternoon hearing
Carlisle United Football Club can confirm that an appeal lodged today against the red card shown to Hallam Hope in the first half of Saturday's away game at Cambridge has been unsuccessful.
Hope went into a challenge with Darnell Furlong on 23 minutes and was shown the card in the wake of what appeared to be a strong and fair tackle.
An FA panel considered the appeal this afternoon but have decided to uphold the decision made by match official Mr Ben Toner.
Hope will now serve a three-match suspension and will miss the Luton, Crawley and Oxford games.


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domain: entertainment


This is a true skeet shooting shell, although its itty-bitty #9 shot pellets are plenty capable of demolishing clays during other types of shooting sports as well. Each trigger pull will launch about 658 dense-patterning and hard-hitting pellets at your target, and NobelSport’s fine vented plastic wad only does more to ensure that you land more shot on target.
NobelSport’s wad also features a hinged base, which collapses at the instant of ignition to keep more kick from reaching your shoulder. This isn’t a hard-kicking shell at all, however, as its clean-burning powder and non-corrosive primer only spark up to produce a 1,145 fps muzzle velocity. You’ll definitely want to keep NobelSport’s smooth-feeding pumpkin-colored shells handy for your next handloading project!
Posted On: 6/8/2022 By: Doug H


text:
The reference guide provides a general understanding of road tolling, types of tolling, its benefits, and the socio-economic reasons for promoting toll road network.
Australian governments have developed national guidelines for the delivery of infrastructure projects to promote cross-government consistency and the use of best practice approaches.
This PPP checklist is an extension of the initial framework.
The report highlights the reasons why benchmarking long-term infrastructure investments has become a sine qua non to match the supply and demand of long-term capital and more.
This second version of the PPP Reference Guide, as the first one, presents a global overview of the diversity of approaches and experiences in the implementation of PPPs and more.
The Reference Guide attempts to provide the most relevant examples, references and resources to help readers inform themselves on key PPP topics.
The Operations Manual presents guidelines and procedures to be followed during the stages of planning, proposal, modeling, approval, contracting and monitoring of the PPP projects within the scope of the State Program of Public-Private Partnerships of Minas Gerais.
The Guide to Guidance is principally aimed at public procurement authorities considering the use of public-private partnership (PPP) arrangements.
The aim of this guidebook is to compile information on PPP frameworks in APEC member economies into a single information as a facilitatory tool for investment.
This handbook provides institutional investors with an overview of opportunities and risks of investing in renewable energy infrastructure.
The OECD Recommendation on Public Procurement is the overarching OECD guiding principle on public procurement that promotes the strategic and holistic use of public procurement.
Public-Private Partnerships (PPPs) are now being used in many countries to develop infrastructure projects.
A practical guide for governments, informed by a country-lens review of leading practices
This handbook synthesises and disseminates knowledge to inform the planning, implementation, and operations of urban rail projects.
G20 finance ministers and central bank governors developed a set of leading practices to promote and prioritize quality infrastructure investment.
The guidelines give concrete advice to countries on how to manage their responsibilities as company owners, thus helping the state-owned enterprises to become more competitive, efficient and transparent.
OECD Checklist for Public Action, the OECD has developed practical guidance organized around 24 OECD principles to help governments and other stakeholders to assess and manage the implications of involving private actors in the financing, development and more.
The OECD Guidelines for Multinational Enterprises provides recommendations to businesses in the areas of disclosure; human rights; employment and industrial relations; environment; combatting bribery and more.
The G20/OECD Principles of Corporate Governance help policy makers evaluate and improve the legal, regulatory, and institutional framework for corporate governance, with a view to supporting economic efficiency, sustainable growth and financial stability.
The OECD Principles for Public Governance of Public-Private Partnerships provide concrete guidance to policy makers on how to make sure that Public-Private Partnerships (PPP) represent value for money for the public sector.

domain: library


text:
Can be build completely out of Lego bricks.
About the MOC
The New Glenn rocket is currently being developed by Blue Origins. with 98 meters in height it will be one of the worlds largest rockets. The first stage will propulsively land to be reused for multiple launches.
Instructions and digital files
Just contact me and I'll make them!


Gunmen have killed 14 truck drivers in an ambush
north of the Iraqi capital Baghdad, said security officials on
The gunmen stopped the drivers early Thursday at a fake checkpoint on a major road linking Baghdad to the northern city of Kirkuk.
They fired at them and made off with their trucks.
The attack was the latest in a surge of violence that has killed nearly 300 people in Iraq since the Muslim holy month of Ramadan began on July 10, according to official figures.
Militants often increase their attacks in Iraq during Ramadan.
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text:
Tufted headboard, cotton bed skirt, white on white bedroom, cream on cream bedroom
We all need more sleep or at least better sleep. This year, new bed and all new cozy, luxy bedding.
Cream and white fall bedroom
Pretty and cozy white bedroom with grey shades rug and fluffy bed cover @pattonmelo
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How to make a bed, layering the linens and pillows to have it look like a magazine photo shoot - sheets, duvet, coverlet, throw, shams, pillows, toss cushions
Love the blend of textures + that they're different shades of white, cream, ivory, etc.
beautiful and girly
Super Stylish Cat Houses, Furniture & Home Essentials For The Discerning Cat Lover


WPS recently replaced the old barrier gate at the Ravelijnsburg complex in Leeuwarden. This project involves retaining the existing access control for entry, while the barrier opens upon exiting the premises based on detection loops.
The complex, dedicated to residents, deploys a remote control for a smooth entry and exit experience. At the request of MVGM and due to positive feedback from residents, this system is has been reused.
Both MVGM and the residents have consistently been satisfied with our service and quality, leading to the renewed choice of WPS for this project. In this assignment, MVGM has once again expressed their satisfaction with the entire process and the end result! This reaffirms that our commitment to reliable parking solutions is appreciated and forms the basis for long-term collaborations.
At WPS, we aim to provide reliable parking solutions that seamlessly integrate with existing systems. If you are interested in enhancing access control to your premises or parking facility with a barrier gate installation, feel free to contact Sander van Kerkoerle who solely specializes in the Dutch market. Alternatively, you can contact your local WPS parking expert. We are ready to assist you in finding the right solution for your situation.
Since its establishment in 1985, WPS Parking Solutions has grown from a local Dutch provider of parking systems into one of the world’s leading providers of cloud-based automated parking solutions. WPS’ mission is to create smart, reliable and future-proof parking solutions that are designed for today’s and tomorrow’s connected world.


text:
Statement Sparkle Crystal Headband
A statement glittering headband made from hand twisted stems of cut crystals, delicate beads and tiny pearls. The design springs from a central motif of clustered crystal for incredible sparkle on your wedding day.
Decoration Length 26cm, 6cm Wide.
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Hunting - Browning Gator Fleece™: Quiet Has Never Been So Tough!
Hunting - MORGAN, UTAH—In partnership with Polartec®/Malden Mills, Browning announces the introduction of a new line of hunting apparel called Gator Fleece™ for 2004. Gator Fleece features a rugged new surface-infusion technology from Polartec®. Tougher than 10-12 oz. canvas duck, the fabric is very abrasion resistant yet quiet enough for any hunting situation, even archery.
Gator Fleece also sheds water and snow and is four times more windproof than regular fleece. Since Gator Fleece has no laminate or coating, it breathes and allows air circulation to eliminate clamminess. Gator Fleece also acts as two garments, an insulating middle layer and a weather shedding outer layer.
Browning Gator Fleece Gator Fleece is also stretchable, allowing for freedom of movement in all directions. A unique design results in less bulk to bind up and hinder body movements and helps to reduce weight. Gator Fleece can be washed time after time without ruining its integrity or colorfastness, meaning camo patterns stay crisp and effective.
Browning Gator Fleece will be offered in a Parka, Jacket, Pant, Glove and Hat in Mossy Oak® New Break-up™ camo. Features include a no-seam shoulder and sleeve design, two upper Ventilator™ pockets with WaterGuard™ zipper closures and two lower cargo pockets with zipper closures, drawcord waist and two-way front zipper closure with inside storm flap. Suggested Retail for Parka, $195.00, Jacket, $170.00, Pant, $150.00, Glove, $23.00 and Hat, $40.00.
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text:
This book wasn’t exactly what I expected.
I thought it would be about Muslims who fled from ISIS controlled areas, and in the process of fleeing to safety, found faith in Jesus.
That in escaping extremist Islam, their experiences not only made them question their own Islamic faith, but through that experience they came to know the love of God through Christ.
At first I thought the title was misleading because it didn’t fulfil that expectation. However, about halfway through I recognised the title had a different kind of application. That recognition came when reading the story of a man, an Iraqi Christian from a Christian community. He tells of experiencing a change:
“…it was as if someone took away all my sadness and gave me another light shining on me. I started a new relationship with Jesus, and I felt like a new man, a new person. I found my hope in Christ. I began to see that in some ways I lost everything when ISIS came to Qaraqosh, but really I found Jesus.”
A related, significant reality I found expressed in this book, is the gaping disconnect between the lives Christians live in the west, and those lived by believers elsewhere.
The man mentioned above didn’t have anything like the prosperity that the west takes for granted, but when he lost what he had, he found something much more valuable; something he thought he already had – and then with the loss of everything else he recognised a sufficiency and wealth only available through closeness to Christ that he’d not experienced before.
There is a vital lesson to be learned by Christians in the west. A lesson that will challenge the seeming obsession with maintaining and protecting a perceived quality of life that is often attributed to God’s blessing. The price of protecting those “blessings” is often a denial of help to people in need, a failure to share those “blessings”.
The author writes of the generosity of the nation of Jordan, who welcomed so many refugees from neighbouring Syria and Iraq, that refugees now made up one in four of the population.
“If that were the United States, it would be like half of Mexico and all of Canada moving in”
Is it necessary to say anything else to address the difference in attitude displayed by western nations with an alleged strong Christian foundation?
The author continues, describing the hardships that have been created,
“…the influx of people looking for cheap accommodations had caused both rents and the prices of staple goods to rise sharply, making life even harder for Jordan’s population. And yet still they open their doors and invite refugees in.”
On questioning a local about the inconvenience of this, he received the reply “What else can we do? Wouldn’t you do the same?”
Sadly most in the west clearly wouldn’t. And neither would many western “Christians”.
I wonder what it will take for THEM to find Jesus.


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From locksmith to limelight: Dujardin, star of 'The Artist,' adored in France(Read article summary)
Jean Dujardin, who has already won a Golden Globe for his role in 'The Artist' and is nominated for an Oscar, has endeared himself – particularly his eyebrows – to the French.
Remy de la Mauviniere/AP
Jean Dujardin, who manages to be both suave and folksy – in a French sort of way – is a 2012 "Best Actor" Oscar nominee for his role in “The Artist,” a black and white “silent” throwback to the 1920s, with swing-era jazz and plenty of playful nostalgia.
Mr. Dujardin, unknown abroad until now, is loved in France as an unsnobby comic who rose from a working class Paris suburb, a one-time locksmith who was told his face was too rotund for the camera.
“I adore him …he is a born clown,” says Christine Bertholts, a legal secretary in Paris, in a typical comment. “And those eyebrows!”
While France has produced several female Oscar winners, Dujardin, will be the first French male to take home the prize if he gets the nod on Feb. 26.
Dujardin plays George Valentin, a silent star with a pencil-thin moustache who can’t or won’t make the transition to talkies and goes into a funk, but is saved by his adorable dog and a woman he generously helps when he’s riding high.


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Founded on lesbian and feminist-centered values; Crescendo: The Tampa Bay Women’s Chorus is an inclusive women’s chorus committed to the performance of music and activities that inspire, educate and unify.
To give voice to the desires and dreams of all people who seek to co-exist in a world of unity and kinship.
AIMS & GOALS
- To create a unique voice that reflects the lives and personalities of our members;
- To exemplify social diversity through education and entertainment;
- To seek the abolishment of all forms of oppression;
- To lead in creating a positive public image;
- To achieve financial stability through fundraising. Crescendo is a chorus first and foremost. Beyond that we are in every sense of the word a family. Outside of the numerous hours spent together rehearsing, singing and performing, we support and affirm the individual paths of each member of our Crescendo family, our chosen community, and the community at large.

domain: sociology


BEIJING (Xinhua) – China has approved the fourth stage of its lunar exploration program, including a basic model of a research station to be built on the Moon in the next decade, according to the China National Space Administration.
Wu Yanhua, deputy director of the administration, said China will carry out lunar exploration in the future Chang’e-6, Chang’e-7 and Chang’e-8 missions.
According to the plans, the Chang’e-7 probe will be launched to the south pole of the Moon first. Because Chang’e-6 is a backup of the Chang’e-5 sample return mission, it will be launched after Chang’e-7 to bring back samples from the lunar south pole. The Chang’e-6 mission will be followed by Chang’e-8, a step toward building a model lunar scientific research station.
“We are also preparing to work with Russia to build the basic model of the lunar research station,” Wu said in a recent interview. “The construction of the station will lay a solid foundation for better exploring the lunar environment and resources, including how to peacefully use and develop lunar resources.”
The Chang’e-5 probe was launched in 2020 and recovered a total of 1,731 grams of lunar samples. China is currently operating the Chang’e-4 lander and the Yutu 2 rover on the far side of the Moon. In 2019, they made the first safe landing on the Von Karman crater in the south polar Aitken Basin, on the far side of the Moon.
Image: The photo, taken by the Yutu-2 (Jade Rabbit-2) rover on Jan. 11, 2019, shows the Chang’e-4 lander. (Courtesy of China National Space Administration)
Xinhua News Agency.© 2000-2019
UyPress – Uruguayan News Agency


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Tributes continued to pour in today for the late publisher of The Guardian newspaper, Alex Ibru, as more Nigerians reacted to the news of his demise.
Governors of Ekiti, Lagos and Ogun States, Dr. Kayode Fayemi, Babatunde Fashola and Ibikunle Amosun, respectively and the Newspaper Proprietors Association of Nigeria, NPAN in separate statements reacted to the death of the former Minister of Internal Affairs and described it as a loss to the nation.
Governor Fayemi said he received the news of Ibruâ€™s death with shock, saying the development had robbed the country of a patriot, a committed statesman who contributed immensely to nation building and welfare of the masses.
The governor, in a statement by his Chief Press Secretary, Mr. Olayinka Oyebode, noted that the legacies of hard work, exemplary leadership and efficient and effective management of resources left behind by Ibru would remain indelible. He urged contemporary leaders in both public and private sectors to emulate Ibruâ€™s humility, discipline, dedication, selfless service to mankind, prudence, probity, transparency, accountability and love for God, which the late businessman brought to bear on governance and business during his tenure.
Fayemi lauded the vigour with which the late Ibru pursued the vision that led to the birth of The Guardian in 1983 and how he was able to nurture it to become the flagship of the Nigerian Press.
According to him, the late Ibru kept a good name both in and out of office, adding that this made him to be one of the most respected statesmen in the country.
The governor also credited Ibru for various reforms he introduced while he served as Minister of Internal Affairs from 1993 to 1995. He also lauded him for establishing the Ibru Centre, which focuses on promotion of religious harmony.
â€œHe was a pragmatic leader, an effective and efficient manager of men and resources. He maintained a good name both in government and business. He also displayed a high sense of altruism and concern for the common man in the course of his public and private life.
â€œIt is sad that Dr. Ibru died at a critical time in the nationâ€™s history when his wise counsel would be needed by many who are willing to tap from his wealth of experience. The late Ibru would be remembered as a highly principled leader and an institution builder,â€ he stated.
Reacting to Ibruâ€™s demise, Fashola said he was too sad to give appropriate words to describe the loss.
â€œI just heard the news as I alighted from the plane. It is very tragic news, very unfortunate. Any passage of this type or any type calls for grief. He is one of the greatest industrialists and politician. He will be greatly missed for his contribution to national development. He was one of the leading lights of Nigeriaâ€™s economic and political landscape,â€ the governor said.
â€œIt is a sad loss to the family. Our sympathy goes to the family with deep sympathy and condolences. I am almost short of words that I cannot say anything now. He was somebody that was very close to me personally. I am shocked really, getting out of the plane and hearing the news,â€ he added.
In his own reaction, Governor Amosun described Ibruâ€™s death as a great loss to Nigeria, pointing out that it is a reminder to all that at the end of it, everyone must be answerable to the Almighty God.
The Newspaper Proprietors Association of Nigeria, NPAN, said it received the news of Ibruâ€™s death with sadness. According to NPAN, Ibru reshaped the Nigerian newspaper landscape with the founding in 1983 of The Guardian newspapers which had as its mission, the desire to be among the five best English language newspapers in the world.
A statement issued and signed by NPAN President, Mr. Nduka Obaigbena, stated that the emergence of the newspaper also came with improved conditions of service for journalists employed in the stable.
â€œHe not only ensured that the newspaper lived up to its motto of â€˜Conscience Nurtured by Truth,â€™ the newspaper under his watch fought against oppression, dictatorship and bad government for a progressive Nigerian state founded on justice.
â€œHe was unarguably one of the most outstanding businessmen in the country who made his mark as part of the famed Ibru brothers whose pre-eminence in the business and economy of Nigeria cannot be understated.
â€œAlex Ibru also lent his wealth of experience to Nigeria when he served as Minister of Internal Affairs between 1993 and 1995. Nigeria will surely miss this champion of conscience nurtured by truth,â€ it said.
Convener, Coalition Against Corrupt Leader, CACOL, Comrade Debo Adeniran said he felt depressed when he heard that Ibru was dead, saying that he was a man who improved the society better than he met it.
According to him, it was unfortunate that he did not see the end of his case against Al-Mustapha and others.
â€œIt is a loss to the nation; it is a loss to the human rights community and it is a loss to the media,â€ he mourned.
Mr. Yinka Odumakin, National Publicity, Afenifere Renewal Group said it is a big loss to the nation, while describing the deceased as a foremost industrialist.
National President, Women Arise, Dr. Joe Okei-Odumakin described the death of Ibru as very painful, saying that he used the media to better the lots of the common man.
â€œWe have lost a reliable Nigerian. Nigeria has lost an illustrious and patriotic man. His death should be a wake-up call to journalists. We condole with his family,â€ she stated.
Lagos lawyer, Festus Keyamo expressed shock at the demise of Ibru. â€œHe came from the family of entrepreneurs,â€ adding that Nigerians would remember him for the role he played in changing the nation.
Human rights activist, Bamidele Aturu described the death of Ibru as sad.
â€œHis creativity in bringing The Guardian to the Nigerian market and defining journalism is something he would be remembered for. Those who want the progress of this nation will surely miss him.
â€œThe wound he suffered during the military era was quite painful. My heart goes to his family; it is a big loss to journalism in Nigeria,â€ he stated.
Secretary, Joint Action Front and former Convener, United Action for Democracy, Comrade Abiodun Aremu said Ibruâ€™s death was a loss to the media world.
According to Afenifere leader, Chief Reuben Fasoranti, â€œIbruâ€™s death is a sad loss to the media as he made lots of contribution to the journalism profession in Nigeria. I hope he has a worthy successor to keep the newspaper going.â€
Former Governor of Kaduna State, Alhaji Balarabe Musa said Ibruâ€™s death was unfortunate and a loss to the nation, the media and the business community in the country.
Publicity Secretary, Action Congress of Nigeria, ACN, Lagos State, Mr. Joe Igbokwe stated that Ibru was made the Minister of Internal Affairs in 1993 by the late General Sani Abacha with the hope that The Guardian will soft-pedal on the illegitimate regime that needed the press desperately to survive.
â€œBut Ibru could not do just that because The Guardian could not bend its philosophy and principle. For this, Mr. Ibru had to pay the price for it. He was shot by Abachaâ€™s goons and killer squad and was thought to be dead. He was battered.
â€œEven though he did not die, then the scars have remained with him, leaving him devastated. Ibru must have died as a result of that devastating attack. Ibru did not go to serve Abacha because he was looking for something to eat but to serve his fatherland.
â€œIt was a patriotic decision that went awry. Ibru would have lived longer but this attack shortened his lifespan. May his soul rest in perfect peace,â€ Igbokwe said.
A federal lawmaker, Dayo Bush-Alebiosu said it was a pity that Ibru had to die at this time, saying that the vacuum he created in the media world would be had to fill.
Another federal lawmaker, Lanre Odubote described Ibru as an accomplished industrialist who paid the utmost price during the military era when he was shot by the Abacha junta.
â€” Kazeem Ugbodaga


text:
Excerpt From The Magic Of RomeNow The Blinds Of The Drawing Room Were Jerked By A Serving Maid, And Then Presently The Marchesa Di Sant Angioli Awoke With A Sigh On Her Lace Pillow And Peered Between The Four Posters At The Bronze Clock A Mercury Held On The Mantelpiece Of Carrara Marbler It Was Her Boast That Her Maid Had Never Found Her Sleeping, And While She Sipped Coffee And Wondered At The Coming Day, Padre Buonavoglio Th R Ew Open His Balcony Window And Whispered A Short Prayer, Seem Ingly To The Stable Roof Rather Than To Heaven, For The Beauty Of God S Morning Had No Private Word For Him, And If He Rose Early And Exercised His Devotions On The Balcony It Was Because It Behoved A Priest Than Because He Enjoyed It.About The PublisherForgotten Books Publishes Hundreds Of Thousands Of Rare And Classic Books Find At Www.forgottenbooks.comThis Book Is A Reproduction Of An Important Historical Work Forgotten Books Uses State Of The Art Technology To Digitally Reconstruct The Work, Preserving The Original Format Whilst Repairing Imperfections Present In The Aged Copy In Rare Cases, An Imperfection In The Original, Such As A Blemish Or Missing Page, May Be Replicated In Our Edition We Do, However, Repair The Vast Majority Of Imperfections Successfully Any Imperfections That Remain Are Intentionally Left To Preserve The State Of Such Historical Works.
Is a well-known author, some of his books are a fascination for readers like in the The Magic of Rome book, this is one of the most wanted Lucas Cleeve author readers around the world.
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- 19 April 2019 Lucas Cleeve


text:
Dr. Marian Betz conducts research in injury prevention, with specific interests
in the role of healthcare providers in (1) older driver safety and (2) suicide prevention. Dr. Betz is currently receiving support from the
National Institute on Aging (through a Paul Beeson Career Development Award) and is the 2014-15 President of the Academy of Geriatric Emergency Medicine (Society of Academic Emergency Medicine). Her current focus is on the use of mixed methods to developed a tiered evaluation system for older drivers,
and she has also worked extensively in the area of “lethal means restriction” for suicide prevention. Dr. Betz also teaches medical and graduate students and residents about injury prevention and geriatrics.

domain: health


text:
Limited Edition minis Patriotic Bucket
Milk Chocolate Toffee Crisp minis Case Pack
Sweet Ground White Chocolate Gourmet Flavored Powder Case Pack
Ghirardelli Premium Whole Bean Coffee
Enjoy a free hot cocoa pouch with every purchase!
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domain: food


Press Release Summary:
Fiber optic UV VIS diode array process spectrometer, OMA-405, suits applications requiring monitoring of single- and multi-component concentrations. With no moving parts, product fits 19 in. rack and is controlled via embedded Windows XP industrial computer with touchscreen interface. Stream composition is obtained through continuous measurement and analysis of spectrum using 1,024-element diode array in wavelength range of 190-800 nm with resolution of 1 nm.
Original Press Release:
Rack Mounted Fiber-Optics UV-VIS Diode Array Process Spectrophotometer
(Maynard MA) Applied Analytics is pleased to announce the introduction of a new rack mounted process analyzer, the OMA-405. The OMA-405 is a UV VIS diode array process spectrometer based on the well-accepted wall-mounted OMA-300 Process Analyzer. The analyzer contains no moving parts and is designed to address on-line applications that require fast, reliable, and maintenance-free monitoring of concentrations, including both single- and multi-components.
The Model OMA-405 Process Analyzer fits a standard 19 rack and is functionally identical to the OMA-300, which is typically housed in an explosion proof enclosure.
The stream composition is obtained through continuous measurement and analysis of a spectrum using a 1024 element diode array, in a wavelength range of 190 nm to 800 nm with a resolution of 1 nm. A broad range of flow cells is available, ranging in path length from 1 mm to 1000 mm, to meet the concentration characteristics of the particular stream.
Typical applications for the OMA-405 include the monitoring of chlorine, trace impurities, metal ions, nitrogenous compounds in exhaust gas, sulfur based components such as H2S and SO2, as well as clean in-place monitoring for pharmaceutical facilities.
The analyzer is controlled via an embedded Windows XP industrial computer with a touch screen user interface. Output signals include 4-20mA, RS232 and Modbus (TCP/IP, Ethernet, serial). A variety of sampling systems are also available.
For more information, please contact sales at Applied Analytics, Inc, 2 Clock Tower Place, Suite 535, Maynard MA 01754, phone: (888) 461-0080, email: firstname.lastname@example.org.


text:
Photographer Stewart Marsden, the official photographer for the London New Year fireworks display and a National Geographic contributor, has written a lengthy tutorial that shows how to capture the best fireworks photographs possible.
Articles tagged "tutorial"
Dec 6, 2021 at 14:00
It's easy to capture hyperlapse videos with the DJI Air 2S, but there's still a bit of planning involved. In this article, we explain the four hyperlapse modes available on the Air 2S and walk you through everything you need to know to shoot your first hyperlapse sequence.
videoAug 4, 2021 at 13:49
Photographer Dustin Dolby has shared an in-depth tutorial showing how you can capture and edit a poster-style commercial shot of beer with minimal effort.
videoDec 26, 2020 at 14:00
Did Santa leave a new Sony camera in your Christmas stocking? If so, your first step should be to enable Sony's 'real-time' autofocus tracking. In this video, we show you how to set up this useful feature.
Oct 18, 2020 at 18:58
All you need is a cool space, a little creative composition and a bit of work in post-production to create these inception-esque drone photos that appear to bend reality.
videoNov 27, 2019 at 14:00
Sony's 'Real-Time' autofocus promises to track almost anything you throw at it, but it's not enabled by default. The words 'Real-Time' don't even appear in the camera's menu. In this video, Chris shows us how to set up this very useful feature.
May 17, 2018 at 15:05
This product photo was captured using two speedlights to light the bottle, a smartphone to light paint the background, and some Photoshop to pull it all together. Watch the video to see how product photographer Dustin Dolby did it.
videoMay 8, 2018 at 14:09
SPOILER ALERT: There's a 'disintegration effect' used at some point in the new Avengers: Infinity War movie. We won't tell you when or why, but Colin Smith of photoshopCAFE will tell you how to recreate this effect on a portrait in Photoshop.
May 2, 2018 at 16:07
In this video, Phlearn's Aaron Nace walks you through his preferred method for sharpening portraits in Photoshop. The best thing about this technique is just how flexible it is: create as many layers of sharpening as you need to get the look that you want.
videoApr 30, 2018 at 15:50
Adobe's 60-second video series of tips and tricks for Lightroom has started making content specifically geared towards users of Adobe's new cloud-centric Lightroom CC.
Apr 25, 2018 at 15:22
Adobe has put together a video tutorial that shows you how to create custom Creative Profiles in Adobe Camera Raw that can be used in ACR, Lightroom CC, and Lightroom Classic CC.
Apr 23, 2018 at 16:24
Open source photo editor GIMP is a popular (and free) Photoshop alternative, but can it really be used on a professional edit? In this video, photographer Shane Milton shows you that it most certainly can.
Apr 17, 2018 at 14:58
As part of his ongoing ‘Good Light’ YouTube series, London-based photographer Sean Tucker has created a simple tutorial on how to find good natural light for portraits.
Apr 16, 2018 at 14:55
You think you know everything there is to know about Lightroom? Think again. Photographer and YouTuber Jamie Windsor thinks he can still teach you a thing or two.
Feb 8, 2018 at 21:53
The complete course is broken down into 25 chapters and includes 8+ hours of photography and retouching tutorials—covering everything from the most basic/classic shots, up to some more creative photography that'll really help you stand out.
Feb 1, 2018 at 20:13
In this tutorial, Phlearn's Aaron Nace covers "the absolute need-to-know fundamentals" of the Pen Tool in Photoshop, so that you can follow along and start mastering this important photo editing tool right away.
Jan 26, 2018 at 18:30
In this video, product photographer Dustin Dolby shows you how to capture a 'classic' perfume product shot using just a single speedlight, some reflective materials, and a bit of lighting know-how.
Dec 1, 2017 at 16:53
Photographer Conner Turmon has put together a useful video tutorial that will get you up to speed on using the RGB tone curves to post-process your photos in either Lightroom or Photoshop.
Sep 29, 2017 at 17:50
In this quick, very informative video, retoucher Jesus Ramirez explains the key differences between Vibrance and Saturation, and demonstrates how both can be used to enhance an image in different ways.
Sep 20, 2017 at 16:18
It might sound like a strange idea, but taking macro photos of boiling water can actually result in some really cool photographs. A good photo experiment for a rainy day.
Sep 3, 2017 at 16:00
In this video, photographer Dustin Dolby offers you a simple, affordable way to capture that classic splash photography look using only cheap speedlights.
Aug 30, 2017 at 17:11
Need a cool photo experiment to try this weekend? Why not try a black-and-white film reversal: transform a black-and-white negative into a direct positive transparency at home.
Aug 21, 2017 at 22:38
In these videos, talented photographer and filmmaker Daniel DeArco breaks down several tips that will help flash photography newbies start experimenting with artificial light.
Aug 19, 2017 at 16:00
This two-part video series takes a deep dive into the world of dynamic symmetry and geometric composition, using iconic photographer Henri Cartier-Bresson's brilliant photographs as a guide.
Aug 15, 2017 at 19:26
You don't need a fancy studio or tons of gear to capture the kind of classic product photography you see in magazines. In this video, Dustin Dolby shows you how to do it with just a couple of speedlights and some know-how.
Aug 8, 2017 at 20:24
This useful, to-the-point tutorial shares a few top tips for posing male models (and friends) so they look better in photos. It's one of the few male posing tutorials we've seen.
Aug 3, 2017 at 14:29
If you're looking to learn Photoshop, or pick up a few nifty new tips, Adobe's got you covered with a brand new YouTube tutorial series called '3, 2, 1 ... Photoshop!'
videoJul 7, 2017 at 20:44
Planning to shoot the eclipse on August 21st? These videos are a great primer on the gear and settings you'll need to do it safely and walk away with a great photo.
Jun 28, 2017 at 18:27
Glassware is one of the most challenging subjects to photograph, especially against a white background. This tutorial shows you how to do it with hardly any gear.
Oct 10, 2016 at 12:00
Winter is coming, but that doesn't mean that you have to hang up your photography gear and head for warmer weather. Here are some tips on what and where to shoot when it gets cold. Read more
Ever wonder how landscape photographers get stunning sharpness throughout their images even when shooting in challenging conditions? The answer is simple, but the process can be difficult. Focus stacking isn't for the faint of heart, but this tutorial should help to shed some light on the process. Learn more
mobileMay 17, 2013 at 18:00
Mobile artist Nettie Edwards harnesses two iOS apps for an old-fashioned effect.
mobileMar 15, 2013 at 13:25
Photographer Star Rush finds sequencing images into movie format useful for thinking through photo essays or exhibitions.
Most popular cameras

domain: hobby


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“With my first 5-year-old, who is 23 now, I wasn’t there. I was working and paying the bills and striving for a better life. Now my youngest is 5, and I can’t leave the house without giving her hugs and kisses. It wasn’t like that with the first because I was just so busy and worried about our future.”… Read More Why are moms treating siblings differently?
From changing jobs to working from home, each working mom in the video explains her own strategy for making sure she has control over the 24 hours in her day.… Read More How moms get their ideal work schedule
Define an area of life you are neglecting and come up with a plan to resolve the issue.… Read More 5 Tips to Achieve Goals for Working Moms
If you’re a working mom feeling overwhelmed by all the demands on your time, you’re not the only one. Your frustration is common, but the way to cope with it is usually not a one-size-fits-all answer. Overcoming the overwhelmed mom syndrome often involves a unique solution that works for your particular situation, whether you are… Read More Tips for a Working Mom Overwhelmed by demands on her time
Ten different working moms reveal their own working mom guilt and how to get over it.… Read More Do you feel working mom guilt?
Why are more moms working in the U.S. than ever before? It’s not just about money. You won’t believe the variety of answers I got when I interviewed a collection of women to find out the reason why moms work more now than ever before. US Department of Labor statistics show 70% of American… Read More Why are more moms working in the US?
Working moms feel we have to tackle every item on our to-do list like a brave fighter ready to defend our position as benevolent but frenzied ruler of the home front and conscientious but shortchanged professional in the work world.… Read More Why is it so hard for women to work and raise kids?


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A bunch of terrific Dallas chefs step away from their stoves to present “A Community Cooks,” a benefit at and for the Farm at Paul Quinn College on April 19.
You’ll recall the school gave up its football field to start an organic garden that just keeps growing to meet the needs of the surrounding neighborhood and teach its students about artisanal farming.
The lineup includes Jeff Harris (Bolsa), Janice Provost (Parigi), Matt McCallister (Campo and FT33), Randall Copeland (Restaurant Ava and Boulevardier), Tim Bevins (Craft), Tre Wilcox (Marquee Grill & Bar), Jason Maddy (Oak), Jon Stevens (Nosh and Snack), Graham Dodds (Central 214), Orazio La Manna (Dallas Cowboys chef) and Brent Hammer (Hibiscus). They’ll donate their time and serve goodies in the garden, just like they did last year (see photo).
It starts with a ribbon-cutting ceremony at 5 p.m. to celebrate the farm’s expansion, and everyone will nosh as the sun sets. With support from the Associate Leadership Council of the Real Estate Council of Dallas, the farm has added a greenhouse, solar lights and an irrigation system. It donates 10 percent of what it grows to the neighborhood.
Tickets are $75 and can be purchased here.


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Event will now take place “virtually,” with family and corporate Walk teams coming together online to “see” each other, while raising funds for type 1 diabetes (T1D) research
Greensboro, North Carolina, March 20, 2020 – JDRF’s Piedmont Triad Chapter announced today that Burlington civic leader John McDonald will serve as Corporate Chair for its “re-imagined” Alamance One Walk on March 28, 2020.
While none of McDonald’s family members live with type 1 diabetes (T1D), he has many friends who are affected by this disease, including former Alamance One Walk Chair Michael Holt and JDRF Piedmont Triad Chapter Board member Anne Hogan, whose son, Andrew, was diagnosed as a small child. That’s why McDonald recognizes the urgent need for research to cure, prevent, and treat type 1.
As the coronavirus spread and it became apparent that hundreds of people could not gather together for the Alamance One Walk as planned, McDonald and the JDRF team sprang into action, creating a “virtual” walk that will allow JDRF supporters in Alamance County to celebrate the great fundraising success that is propelling JDRF’s research progress toward curing T1D.
All Alamance Walk Teams and individual walkers are invited to celebrate JDRF’s vision of a world without type 1 on March 28 via social media. The Piedmont Triad Chapter is encouraging all those who were planning to walk to:
- Put on their Walk team t-shirts (many of which highlight this year’s theme of Dr. Seuss on the Loose), lace up their sneakers, and get moving wherever they are—walking around the block, hopping on a treadmill, or jogging around their neighborhood.
- Take a video or snap a photo, sharing it on social media with the hashtags #ManyForOneWalk and #JDRFOneWalk and tagging:
- JDRF’s Piedmont Triad Chapter, by using @JDRFTriad on Facebook and Twitter;
- Broadcast partner FOX8, by using @FOX8news on Facebook and @myfox8 on Twitter; and
- Print partner the Times-News, by using @TimesNewsBurlington on Facebook.
At 9 am on Saturday, March 28, JDRF’s Piedmont Triad Chapter will share the Alamance Many For One Walk opening ceremonies at Facebook.com/JDRFTriad. Stay tuned to Facebook throughout the day to see how people around Alamance County, the Piedmont Triad, and beyond are joining in to create a world without T1D.
“While—by necessity—March 28 has to be different than previous Walk Days, we want to make sure our Alamance Walk teams still have an opportunity to ‘see’ all their teammates—and all the other folks they’d normally celebrate with in person. And that’s why we’re so excited about our virtual Alamance ‘Many For One Walk,’” said JDRF Piedmont Triad Chapter Executive Director Eleanor Schaffner-Mosh. “Please join us online starting at 9 am on March 28 and let’s celebrate how the Alamance community is coming together to turn type one into type none!”
With more than $100,000 already raised by Alamance Walk teams and individuals, Walk Chair John McDonald said, “I’m truly thankful for the remarkable work our Walk teams are doing in the face of these uncertain times to continue raising much-needed funds to cure type 1 diabetes. What a wonderful spirit of giving we have here in Alamance County! I know we’re all looking forward to participating in our virtual walk on Saturday morning, March 28!”
To learn more about the Alamance Walk and make donations to help JDRF cure T1D, please visit https://tinyurl.com/JDRFAlamance2020.
2020 Alamance One Walk Sponsors: JDRF thanks our Alamance sponsors, many of whom have supported T1D research for years. Major 2020 sponsors committed to date include:
Presenting Media Sponsor
- FOX8 – WGHP
- Cone Health/Alamance Regional Medical Center
- Glen Raven
- Buckner HeavyLift Cranes
- Harris Crouch
- Elon University
- American National Bank
- Chandler Concrete
- Gilliam Bell Moser
- Kernodle Clinic
- Pro Feet
- Stearns Ford/County Ford
- The Times-News
- Trust Company of the South
About Type 1 Diabetes: Type 1 diabetes (T1D) is an autoimmune disease that occurs when a person’s pancreas stops producing insulin, the hormone that controls blood-sugar levels. T1D develops when the insulin-producing pancreatic beta cells are mistakenly destroyed by the body’s immune system. The cause of this attack is still being researched; however, scientists believe the cause may have genetic and environmental components. T1D affects children and adults, and people can be diagnosed at any age. As of now, there is nothing anyone can do to prevent T1D, and there is no cure.
About JDRF: JDRF is the leading global organization funding type 1 diabetes (T1D) research. Our mission is to improve lives today and tomorrow by accelerating life-changing breakthroughs to cure, prevent, and treat T1D and its complications. To accomplish this, JDRF has invested more than $2 billion in research funding since our inception. We are an organization built on a grassroots model of people connecting in their local communities, collaborating regionally for efficiency and broader fundraising impact, and uniting on a national stage to pool resources, passion, and energy. We collaborate with academic institutions, policymakers, and corporate and industry partners to develop and deliver a pipeline of innovative therapies to people living with T1D. Our staff and volunteers throughout the United States and our six international affiliates are dedicated to advocacy, community engagement, and our vision of a world without T1D. Learn more at jdrf.org or @JDRF on Twitter.
About JDRF – Piedmont Triad Chapter: Covering 15 North Carolina counties, the Piedmont Triad Chapter provides support for those living with type 1 diabetes by convening educational and networking sessions such as the annual TypeOneNation Summit, and raises awareness and funds for T1D research through a variety of events, including our annual Hope Gala; One Walks in Winston-Salem, Greensboro, Alamance County, and High Point; the Ride to Cure Diabetes; and the Tennis to Tent to Tee event in Pinehurst. Learn more at jdrf.org/triad, facebook.com/jdrftriad, or @JDRFtriad on Twitter.


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Project description:Here we present the complete genome sequence of Lactobacillus acidophilus ATCC 53544. The assembly contains 1,991,906 bp and is 99.7% similar to L. acidophilus NCFM. This strain was isolated from a rectal swab specimen of an infant and has previously been used as a feed supplement for animals.
Project description:We present the 1,991,830-bp complete genome sequence of Lactobacillus acidophilus strain La-14 (SD-5212). Comparative genomic analysis revealed 99.98% similarity overall to the L. acidophilus NCFM genome. Globally, 111 single nucleotide polymorphisms (SNPs) (95 SNPs, 16 indels) were observed throughout the genome. Also, a 416-bp deletion in the LA14_1146 sugar ABC transporter was identified.
Project description:Lactobacillus acidophilus (L. acidophilus) is a representative probiotic and is widely used in many industrial products for its beneficial effects on human and animal health. This bacterium is exposed to harsh environments such as high temperatures for manufacturing industrial products, but cell yield under high temperatures is relatively low. To resolve this issue, we developed a new L. acidophilus strain with improved heat resistance while retaining the existing beneficial properties through the adaptive laboratory evolution (ALE) method. The newly developed strain, L. acidophilus EG008, has improved the existing limit of thermal resistance from 65°C to 75°C. Furthermore, we performed whole-genome sequencing and comparative genome analysis of wild-type and EG008 strains to unravel the molecular mechanism of improved heat resistance. Interestingly, only two single-nucleotide polymorphisms (SNPs) were different compared to the L. acidophilus wild-type. We identified that one of these SNPs is a non-synonymous SNP capable of altering the structure of MurD protein through the 435th amino acid change from serine to threonine. We believe that these results will directly contribute to any industrial field where L. acidophilus is applied. In addition, these results make a step forward in understanding the molecular mechanisms of lactic acid bacteria evolution under extreme conditions.
Project description:Lactobacillus acidophilus 30SC has been isolated from swine intestines and considered a probiotic strain for dairy products because of its ability to assimilate cholesterol and produce bacteriocins. Here, we report the complete genome sequence of Lactobacillus acidophilus 30SC (2,078,001 bp) exhibiting strong acid resistance and enhanced bile tolerance.
Project description:Lactobacillus acidophilus is one of the most commonly used industrial products worldwide. Since its probiotic efficacy is strain-specific, the identification of probiotics at both the species and strain levels is necessary. However, neither phenotypic nor conventional genotypic methods have enabled the effective differentiation of L. acidophilus strains. In this study, a whole-genome sequence-based analysis was carried out to establish high-resolution strain typing of 41 L. acidophilus strains (including commercial isolates and reference strains) using the cano-wgMLST_BacCompare analytics platform; consequently, a strain-specific discrimination method for the probiotic strain LA1063 was developed. Using a core-genome multilocus sequence-typing (cgMLST) scheme based on 1390 highly conserved genes, 41 strains could be assigned to 34 sequence types. Subsequently, we screened a set of 92 loci with a discriminatory power equal to that of the 1390 loci cgMLST scheme. A strain-specific polymerase chain reaction combined with a multiplex minisequencing method was developed based on four (phoU, secY, tilS, and uvrA_1) out of 21 loci, which could be discriminated between LA1063 and other L. acidophilus strains using the cgMLST data. We confirmed that the strain-specific single-nucleotide polymorphisms method could be used to quickly and accurately identify the L. acidophilus probiotic strain LA1063 in commercial products.
Project description:A set of lactobacilli were investigated by polyphasic analysis. Multilocus sequence analysis, DNA typing, microarray analysis, and in silico whole-genome alignments provided a remarkably consistent pattern of similarity within the Lactobacillus acidophilus complex. On microarray analysis, 17 and 5% of the genes from Lactobacillus johnsonii strain NCC533 represented variable and strain-specific genes, respectively, when tested against four independent isolates of L. johnsonii. When projected on the NCC533 genome map, about 10 large clusters of variable genes were identified, and they were enriched around the terminus of replication. A quarter of the variable genes and two-thirds of the strain-specific genes were associated with mobile DNA. Signatures for horizontal gene transfer and modular evolution were found in prophages and in DNA from the exopolysaccharide biosynthesis cluster. On microarray hybridizations, Lactobacillus gasseri strains showed a shift to significantly lower fluorescence intensities than the L. johnsonii test strains, and only genes encoding very conserved cellular functions from L. acidophilus hybridized to the L. johnsonii array. In-silico comparative genomics showed extensive protein sequence similarity and genome synteny of L. johnsonii with L. gasseri, L. acidophilus, and Lactobacillus delbrueckii; moderate synteny with Lactobacillus casei; and scattered X-type sharing of protein sequence identity with the other sequenced lactobacilli. The observation of a stepwise decrease in similarity between the members of the L. acidophilus group suggests a strong element of vertical evolution in a natural phylogenetic group. Modern whole-genome-based techniques are thus a useful adjunct to the clarification of taxonomical relationships in problematic bacterial groups.
Project description:Lactobacillus acidophilus is a gram-positive, microaerophilic, and acidophilic bacterial species. L. acidophilus strains in the gastrointestinal tracts of humans and other animals have been profiled, but strains found in the canine gut have not been studied yet. Our study helps in understanding the genetic features of the L. acidophilus C5 strain found in the canine gut, determining its adaptive features evolved to survive in the canine gut environment, and in elucidating its probiotic functions. To examine the canine L. acidophilus C5 genome, we isolated the C5 strain from a Korean dog and sequenced it using PacBio SMRT sequencing technology. A comparative genomic approach was used to assess genetic relationships between C5 and six other strains and study the distinguishing features related to different hosts. We found that most genes in the C5 strain were related to carbohydrate transport and metabolism. The pan-genome of seven L. acidophilus strains contained 2,254 gene families, and the core genome contained 1,726 gene families. The phylogenetic tree of the core genes in the canine L. acidophilus C5 strain was very close to that of two strains (DSM20079 and NCFM) from humans. We identified 30 evolutionarily accelerated genes in the L. acidophilus C5 strain in the ratio of non-synonymous to synonymous substitutions (dN/dS) analysis. Five of these thirty genes were associated with carbohydrate transport and metabolism. This study provides insights into genetic features and adaptations of the L. acidophilus C5 strain to survive the canine intestinal environment. It also suggests that the evolution of the L. acidophilus genome is closely related to the host's evolutionary adaptation process.
Project description:Many Lactobacillus species are frequently isolated from dairy products, animal guts, and the vaginas of healthy women. However, sequencing-based identification of isolated Lactobacillus strain is time/cost-consuming and lobor-intensive. In this study, we developed a multiplex PCR method to distinguish six closely related species in the Lactobacillus acidophilus group (L. gasseri, L. acidophilus, L. helveticus, L. jensenii, L. crispatus, and L. gallinarum), which is based on species-specific primer sets. Altogether, 86 genomes of 9 Lactobacillus species from the National Center of Biotechnology Information (NCBI) database were compared to detect species-specific genes and design six species-specific primer sets. The PCR conditions of the individual primer sets were optimized via gradient PCR methods. A final multiplex PCR condition was also optimized for a mixture of all six primer sets mixed. When identifying a single strain, the optimized multiplex PCR method can specifically detect one of the six species, but no band was amplified at least from the other Lactobacillus and Enterococcus species. These results indicated that species-specific primer sets designed from the genome comparison could identify one strain within the six Lactobacillus species by a single PCR reaction. Using the method described here, we will be able to save time, cost, and labor during species identification and screening of commercially important probiotic lactobacilli.
Project description:A new Lactobacillus acidophilus strain, FSI4, isolated from yogurt, was isolated and sequenced in our laboratory. Our data, although supportive of previous conclusions regarding the remarkable stability of L. acidophilus species, indicate accumulating mutations in commercial L. acidophilus strains that warrant further study of the effect of damaged genes on the competitiveness of these bacteria in gut microbiota.
Project description:Helicobacter pylori causes chronic gastritis and avoids elimination by the immune system of the infected host. The commensal bacterium Lactobacillus acidophilus has been reported to exert beneficial effects as a supplement during H. pylori eradication therapy. In the present study, we applied whole genome microarray analysis to compare the immune response induced in murine bone marrow derived macrophages (BMDM) stimulated with L. acidophilus, H. pylori, or with both bacteria in combination Microarray expression profiling was performed to analyze stimulation of bone marrow derived macrophages with Helicobacter pylori 251, Lactobacillus acidophilus NCFM or Lactobacillus acidophilus NCFM co-stimulated with Helicobacter pylori 251 were analyzed 5 hours after infection.

domain: biology


President Barack Obama dodged questions Thursday about the IRS's targeting of conservative groups, shifted responsibility for the Benghazi attack to Congress, and said 'I offer no apologies' for the Department of Justice's secret seizure of reporter's phone records in search of a classified intelligence leak.
In a rain-soaked Rose Garden press conference originally intended to be a victory lap for the United States' relationship with Turkey, Obama stood alongside Turkish Prime Minister Recep Erdogan and fielded questions which quickly shifted to the trio of scandals that are engulfing his administration.
Beginning in 2010, Obama's IRS targeted a list of approximately 300 tea party-related and other conservative groups for aggressive scrutiny following their applications for tax-exempt status.
His Department of Justice secretly spied on the Associated Press's phone records in an attempt to trace a national security leak.
And his State Department political appointees intervened in the aftermath of the 2012 terror attack on an American diplomatic outpost in Libya, in a process that resulted in a misleading set of talking points which ignored terrorism in favor of a more muted explanation, in the midst of a re-election campaign.
On the IRS scandal, Obama wouldn't commit to saying no one at the White House knew about the targeting of conservative groups before the White House Counsel was informed just days ago.
'I certainly did not know anything about the IG report before the IG report was leaked to the press,' he said, without addressing the question of whether any of his political appointees in the West Wing of the White House were aware that the IRS was playing political favorites.
Read more: http://www.dailymail.co.uk/news/arti...#ixzz2TTxg0843
Follow us: @MailOnline on Twitter | DailyMail on Facebook


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Meatballs baked in rich tomato sauce with a melting mozzarella topping - this recipe is all about comfort food. It's also perfect for freezing, making it a great option for when you don't want to cook mid-week.
Cornflake & Malteser Rocky Road - A delicious twist on a classic – Cornflake & Malteser Rocky Road! Perfect for a party, or just as a good snack! www.janespatisserie.com
JamieOliver.com is your one stop shop for everything Jamie Oliver including delicious and healthy recipes inspired from all over the world, helpful food tube videos and much more.
Easy Prawn Cocktail Recipe with free child friendly printable recipe sheet - easy recipe for kids from Eats Amazing UK
Easy fruity mini pancake stackers – kids will love these fun and healthy pancakes kebabs for breakfast snack or dessert – Eats Amazing UK
Half-term adventures just got more fun with these tasty apple nachos. Made in just three super simple steps, this snack recipe for kids is exactly what long holidays call for. | Tesco
These Pesto Parmesan Pastry Straws make a super easy snack or party appetizer for the festive season!
Pin it to save it for later ! Farsi Khaman Puri, an another creation of mine, flavourful and delicious! Inspired by my favourite Diwa...
Filo pastry mini quiches - great for kids to make and eat! - A Mummy Too
Gingerbread - These are great for children to help make and are ideal after school snacks...


The latest batch of Dell Latitude 5580's that I've set up won't connect to our wifi network. The laptops work on other (home) networks. Other dells connect fine. I think even some older 5580's. I've run dell command to update all the drivers and firmware. I've rolled back the wifi driver version. Nothing seems to work. Any suggestions?
Latest firmware on the unifis as well?