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The impressive ability of palladium to assemble C-C bonds between appropriately functionalized substrates has allowed synthetic organic chemists to perform transformations that were previously impossible or only possible using multi-step approaches. In this context, one of the most important and elegant applications of the Pd-catalyzed C-C coupling reactions currently is the synthesis of pharmaceuticals. This review is intended to give a picture of the applications of Pd-catalyzed C-C cross-coupling reactions for the synthesis of drug components or drug candidates regardless of the scale from 2011 through to the end of July, 2014.
cross-coupling reactions; Heck reactions; palladium; pharmaceuticals
A grande capacidade do paládio de formar ligações carbono-carbono entre substratos apropriadamente funcionalizados permitiu que os químicos orgânicos efetuassem transformações antes impossíveis ou alcançáveis somente através de rotas muito longas. Neste contexto, uma das mais elegantes e importantes aplicações das reações de acoplamento cruzado catalisadas por paládio é a síntese de compostos de interesse farmacêutico. A presente revisão tem por objetivo apresentar uma visão geral do uso de acoplamentos cruzados na síntese de componentes de medicamentos (ou de candidatos a medicamentos), independentemente da escala, compreendendo o período de 2011 até o final de julho de 2014.
Palladium-catalyzed cross-coupling reactions comprise one of the most efficient methods for the construction of carbon-carbon bonds.11 Miyaura, N.; Suzuki, A.; Chem. Rev.1995, 95 , 2457.
2 Beletskaya, I. P.; Cheprakov, A. V.; Chem. Rev.2000, 100 , 3009.-33 Chinchilla, R.; Najera, C.; Chem. Soc. Rev.2011, 40 , 5084. The impressive ability of palladium to assemble C-C bonds between appropriately functionalized substrates has allowed synthetic organic chemists to perform transformations that were previously impossible or only possible using multi-step approaches. Therefore, these cross-coupling methodologies have revolutionized the way of thinking regarding synthetic organic chemistry, having found widespread use in organic synthesis and material science, as well playing an important role in pharmaceutical, agrochemical and fine chemical industries.44 Magano, J.; Dunetz, J. R.; Chem. Rev.2011, 111 , 2177.
5 Torborg, C.; Beller, M.; Adv. Synth. Catal.2009, 351 , 3027.
6 Nicolaou, K. C.; Bulger, P. G.; Sarlah, D.; Angew. Chem., Int. Ed.2005, 44 , 4442.
7 Corbet, J.-P.; Mignani, G.; Chem. Rev.2006, 106 , 2651.-88 Roughley, S. D.; Jordan, A. M.; J. Med. Chem.2011, 54 , 3451. In this scenario, the award of the Nobel Prize in Chemistry in 2010 was a deserved recognition, rewarding jointly Richard F. Heck, Ei-ichi Negishi99 Negishi, E.-I.; Angew. Chem., Int. Ed.2011, 50 , 6738. and Akira Suzuki1010 Suzuki, A.; Angew. Chem., Int. Ed.2011, 50 , 6722. for their remarkable contributions for the Pd-catalyzed cross-couplings in organic synthesis.1111 Johansson Seechurn, C. C. C.; Kitching, M. O.; Colacot, T. J.; Snieckus, V.; Angew. Chem., Int. Ed.2012, 51 , 5062.
The generally accepted mechanisms for Pd-catalyzed C-C cross-coupling reactions are summarized in Scheme 1. All reactions start with a Pd zerovalent species preformed or formed in situ from Pd divalent catalyst precursors. The first step is common for all reactions, consisting of the oxidative addition reaction of organic halide (or pseudohalide) to the Pd zerovalent species affording the R11 Miyaura, N.; Suzuki, A.; Chem. Rev.1995, 95 , 2457.-Pd-X intermediate. In the case of coupling reactions involving an organometallic partner (R22 Beletskaya, I. P.; Cheprakov, A. V.; Chem. Rev.2000, 100 , 3009.M), the R11 Miyaura, N.; Suzuki, A.; Chem. Rev.1995, 95 , 2457.-Pd-R22 Beletskaya, I. P.; Cheprakov, A. V.; Chem. Rev.2000, 100 , 3009. intermediate is formed by a transmetalation step. Finally, reductive elimination provides the R11 Miyaura, N.; Suzuki, A.; Chem. Rev.1995, 95 , 2457.-R22 Beletskaya, I. P.; Cheprakov, A. V.; Chem. Rev.2000, 100 , 3009. coupling product, and regenerates the Pd zerovalent species. The Sonogashira reaction involves the coupling between organic halides and alkynes. When copper salts are used as the co-catalyst, copper acetylides are formed in situ and are involved in the transmetalation step. A different catalytic cycle is involved in the Heck-Mizoroki reaction. In this case, the alkene is coordinated to the common R11 Miyaura, N.; Suzuki, A.; Chem. Rev.1995, 95 , 2457.-Pd-X intermediate. Then, a syn migratory insertion occurs and the organopalladium species formed undergoes β-hydride elimination to form the alkene product. Finally, a base-assisted elimination of HX regenerate the Pd(0) catalyst.1212 Bräse, S.; Meijere, A. D. In Metal-Catalyzed Cross-Coupling Reactions ; Wiley-VCH Verlag: GmbH, 2008, p. 217.
13 Echavarren, A. M.; Cárdenas, D. J. In Metal-Catalyzed Cross-Coupling Reactions ; Wiley-VCH Verlag: GmbH, 2008, p. 1.
14 Marsden, J. A.; Haley, M. M. In Metal-Catalyzed Cross-Coupling Reactions ; Wiley-VCH Verlag: GmbH, 2008, p. 317.
15 Mitchell, T. N. In Metal-Catalyzed Cross-Coupling Reactions ; Wiley-VCH Verlag: GmbH, 2008, p. 125.
16 Miyaura, N. In Metal-Catalyzed Cross-Coupling Reactions ; Wiley-VCH Verlag: GmbH, 2008, p. 41.-1717 Negishi, E.-I.; Zeng, X.; Tan, Z.; Qian, M.; Hu, Q.; Huang, Z. In Metal-Catalyzed Cross-Coupling Reactions ; Wiley-VCH Verlag: GmbH, 2008, p. 815. When an aryldiazonium salt is used in place of an aryl halide, the reaction is known as Heck-Matsuda reaction.1818 Taylor, J. G.; Moro, A. V.; Correia, C. R. D.; Eur. J. Org. Chem.2011, 2011 , 1403.
19 Bonin, H.; Fouquet, E.; Felpin, F.-X.; Adv. Synth. Catal.2011, 353 , 3063.
20 Roglans, A.; Pla-Quintana, A.; Moreno-Mañas, M.; Chem. Rev.2006, 106 , 4622.-2121 Sabino, A. A.; Machado, A. H. L.; Correia, C. R. D.; Eberlin, M. N.; Angew. Chem., Int. Ed.2004, 43 , 2514. The difference in terms of mechanism is that the oxidative addition of aryldiazonium salts to the Pd zerovalent species generates a cationic [R1-Pd]+ intermediate.
In our opinion, the most important and elegant application of the coupling reactions is the synthesis of pharmaceuticals. In this context, there is an excellent review about the applications of Pd-catalyzed coupling reactions. This review covers the period from 2001 to 2008, and highlights examples that have been performed on at least a kilogram scale in the chemical and pharmaceutical industries.55 Torborg, C.; Beller, M.; Adv. Synth. Catal.2009, 351 , 3027. In addition, Pfizer researchers have reviewed the large-scale applications of transition metal-catalyzed coupling reactions for the manufacture of drug components in the pharmaceutical industry through to the end of August, 2010.44 Magano, J.; Dunetz, J. R.; Chem. Rev.2011, 111 , 2177. This review is intended to give a picture of the applications of Pd-catalyzed C-C cross-coupling reactions for the synthesis of drug components or drug candidates, regardless of scale, from 2011 through to the end of July, 2014.
2. Suzuki-Miyaura Reactions
The transition metal-catalyzed reaction between an organoboron compound and a halide or pseudohalide is known as Suzuki-Miyaura cross-coupling.2222 Suzuki, A.; Pure Appl. Chem.1985, 57 , 1749. This important reaction is well-known for the assembly of biphenyl motifs.2323 Han, F.-S.; Chem. Soc. Rev.2013, 42 , 5270. For example, Dalby et al.2424 Dalby, A.; Mo, X.; Stoa, R.; Wroblewski, N.; Zhang, Z.; Hagen, T. J.; Tetrahedron Lett.2013, 54 , 2737. reported a concise synthesis of D159687 (1), a partial allosteric modulator of phosphodiesterase 4 (PDE4), an enzyme related to inflammatory and respiratory diseases. The first synthesis of 1 involved six steps, with one Suzuki reaction step, with an overall yield of 8%.2525 Burgin, A.; Gurney, M.; Kiselyov, A.; Magnusson, O.; Singh, J.; WO2010059838-A2, WO2010059838-A3,2009. By using the benzyl aryl dibromide2 as starting material, the synthesis of 1 was shortened to two-steps with an overall yield of 47% on the gram scale (Scheme 2).
The more reactive benzyl bromide moiety of dibromide 2 was coupled with a pinacol arylboronic ester, providing diarylmethane 3 in 66% yield. Activation of the remaining bromide in 3 was carried out using the same conditions, only changing the temperature and reaction time, and furnishing 1 at a yield of 71%. The optimal conditions displayed in Scheme 2 were established after a screening of Pd sources [Pd(PPh3)4, PdCl2(PPh3)2 and Pd(OAc)2/PPh3], solvents [dimethoxyethane/EtOH/H2O, dioxane/H2O and dimethylformamide (DMF)] and bases (K3PO4, K2CO3, Na2CO3 and Cs2CO3). It is worth noting that a reduction of the amount of catalyst increased the conversion in some cases, while higher amounts generally increased the level of impurities and difficult purification. This process was applied to the synthesis of two new PDE4D partial allosteric modulators, D159404 (4) and D159153 (5) (Figure 1), with overall yields of around 26% for the two Suzuki reactions.
Thiel et al. described the preparation of several inhibitors of p38 mitogen-activated protein (MAP) kinases, which are enzymes that positively regulate the production of pro-inflammatory mediators (TNF-α and IL-1).2626 Thiel, O. R.; Achmatowicz, M.; Milburn, R. M.; Synlett2012, 23, 1564. These mediators are involved in rheumatoid arthritis, Crohn's disease and psoriasis. The original synthetic route2727 Herberich, B.; Cao, G.-Q.; Chakrabarti, P. P.; Falsey, J. R.; Pettus, L.; Rzasa, R. M.; Reed, A. B.; Reichelt, A.; Sham, K.; Thaman, M.; Wurz, R. P.; Xu, S.; Zhang, D.; Hsieh, F.; Lee, M. R.; Syed, R.; Li, V.; Grosfeld, D.; Plant, M. H.; Henkle, B.; Sherman, L.; Middleton, S.; Wong, L. M.; Tasker, A. S.; J. Med. Chem.2008, 51 , 6271. presented some challenges for scaling-up, most notably the employment of toxic carbon tetrachloride, silver salts and microwave irradiation. Therefore, the authors optimized the route, on a kilogram scale, employing the aryl chloride 6 as starting material and avoiding the use of toxic solvents (Scheme 3). For activation of the less reactive C-Cl bond, ligand screening was necessary, and MePhos (7) was identified as the most effective. Thus, this ligand combined with a mixture of ethanol/aqueous sodium hydroxide was employed with Pd2(dba)3, where dba = dibenzalacetone, as pre-catalyst to provide 8 in yields between 70 and 81%. In contrast to the medicinal chemistry route, the final step did not employ a palladium-catalyzed reaction, allowing easier removal of this metal: the synthesis of 9 employed 1,1-carbonyldiimidazole (CDI) and cyclopropylamine in ethyl acetate leading to the desired product in 70% yield after two steps. The authors believe that any significant amount of palladium present as an impurity in intermediate 8 was removed quantitatively with the aid of imidazole employed in the carbonyl activation step.
It is worth noting that the large scale synthesis of 10, another inhibitor of p38 MAP kinases, demanded a completely different catalytic system for the Suzuki reaction. While the original medicinal chemistry route employed Pd(PPh3)4 for the coupling of 11 with a boron pinacolate derivative, on a large scale, the authors were capable of reducing the catalyst loading (albeit no amounts were disclosed in the text) by using PdCl2(APhos) (12), allowing a very clean reaction to be performed due to the high activity of this catalyst (Scheme 4).2626 Thiel, O. R.; Achmatowicz, M.; Milburn, R. M.; Synlett2012, 23, 1564. Thus, the Suzuki product 13 was obtained in 93% yield by treating 11 with the appropriate arylboronic acid in the presence of 12 and potassium phosphate, with a mixture of isopropyl alcohol and water as solvent. Finally, treatment of 13 with CDI in tetrahydrofuran (THF) provided 10 in 94% yield.
Gillmore et al. developed a multi-kilogram preparation of PF-01367338 (14), a drug candidate for the treatment of breast and ovarian cancers.2828 Gillmore, A. T.; Badland, M.; Crook, C. L.; Castro, N. M.; Critcher, D. J.; Fussell, S. J.; Jones, K. J.; Jones, M. C.; Kougoulos, E.; Mathew, J. S.; McMillan, L.; Pearce, J. E.; Rawlinson, F. L.; Sherlock, A. E.; Walton, R.; Org. Process Res. Dev.2012, 16 , 1897. It is an inhibitor of poly(ADP ribose) polymerase (PARP), an enzyme that is responsible for repairing damaged DNA in normal and tumour cells.2828 Gillmore, A. T.; Badland, M.; Crook, C. L.; Castro, N. M.; Critcher, D. J.; Fussell, S. J.; Jones, K. J.; Jones, M. C.; Kougoulos, E.; Mathew, J. S.; McMillan, L.; Pearce, J. E.; Rawlinson, F. L.; Sherlock, A. E.; Walton, R.; Org. Process Res. Dev.2012, 16 , 1897. The described synthetic route delivered 2 kg of the salt 14, and the authors described how they had to overcome some synthetic challenges in order to scale up the synthesis to multi-kilogram scale. The overcome problems include: understanding of the thermal hazards of the Leimgruber-Batcho indole synthesis, installation of the side chain through a reductive alkylation procedure, improvements in the robustness of the Suzuki coupling, removal of hydrogen cyanide generation during a reductive amination reaction, and reliable generation of good-quality free base for the final salt formation step.
The Suzuki reaction between the tricyclic bromide 15 and 4-formylphenylboronic acid was first carried out in multi-kilogram scale using by Pd(PPh3)4 as catalyst. The reaction did not reach completion requiring an additional catalyst charge. In the sequence, the Suzuki reaction was performed with [PdCl2(dppf)·CH2Cl2] as a pre-catalyst, and full conversion was obtained after 2 h at 90 ºC (Scheme 5), providing 16 with yield of 92%.
Grongsaard et al. reported a scalable synthetic route of 17, an inhibitor of Akt kinase, which is an enzyme that is up-regulated in several types of cancer, such as colon, breast, brain, lung and prostate.2929 Grongsaard, P.; Bulger, P. G.; Wallace, D. J.; Tan, L.; Chen, Q.; Dolman, S. J.; Nyrop, J.; Hoerrner, R. S.; Weisel, M.; Arredondo, J.; Itoh, T.; Xie, C.; Wen, X.; Zhao, D.; Muzzio, D. J.; Bassan, E. M.; Shultz, C. S.; Org. Process Res. Dev.2012, 16 , 1069. This interesting polycyclic was prepared on small scale through a Suzuki reaction between a pyridyl chloride and an aryl boronate. However, given the harsh conditions required for this reaction [140 ºC, microwave heating, 20 mol% of Pd(PPh3)4], an improvement of this step was clearly necessary for scale-up. Thus, an optimization study was performed for the Suzuki reaction between triflate 18 and boron pinacolate 19, with a screening of ligands [XPhos, 1,4-bis(diphenylphosphino)butane (dppb), (R)-(2,2'-bis(diphenylphosphino)-1,1'-binaphthyl) (BINAP), 1,1'-bis(diphenylphosphino)ferrocene (dppf) and 1,1'-bis(di-i-propylphosphino)ferrocene (dippf)]. Electron-rich, ferrocen-derived phosphines presented superior performances (Scheme 6). Also, a mixture of THF and water was found to be the optimal solvent, but 2-MeTHF was chosen instead of THF because enables a simpler purification by crystallization. Notably, water proved to be essential to this transformation, the yields were lower in anhydrous media. However, the authors did not provide an explanation for this. Optimal conditions were found and are shown in Scheme 6: 3 mol% of both Pd(OAc)2 and dippf (20) were employed with potassium phosphate as base and a mixture of 2-MeTHF and water as solvent, with the heating temperature steadily raised from room temperature to 50 ºC. The desired product 21 was obtained in 84% yield, and removal of the tert-butyloxycarbonyl (Boc)-protecting group afforded 17 in 93% yield.
Synthesis of 17 by means of a Suzuki reaction with the advanced boron pinacolate intermediate 19.
In the Suzuki reaction mentioned above, the valuable boron pinacolate 19 was employed only in slight excess. Even though borylations are out of the scope of this review, the synthesis of 19 deserves particular mention, since this compound demanded more than ten steps to be reached starting from acid 22 and was finally obtained by an elegant Pd-catalyzed borylation of the aryl chloride 23 (Scheme 7).
Tian et al. described a convergent synthesis for GDC-09413030 Tian, Q.; Cheng, Z.; Yajima, H. M.; Savage, S. J.; Green, K. L.; Humphries, T.; Reynolds, M. E.; Babu, S.; Gosselin, F.; Askin, D.; Kurimoto, I.; Hirata, N.; Iwasaki, M.; Shimasaki, Y.; Miki, T.; Org. Process Res. Dev.2013, 17 , 97. (24), a phosphatidylinositol 3-kinase (PI3K) inhibitor (Scheme 8). The PI3K pathway is frequently activated in tumours and its inhibition has a role in human cancers. The indazole-derived boronic acid 26 was employed as its tetrahydropyran-protected derivative due to solubility issues and to render an easier purification. Previous studies identified Pd- or Ni-catalyzed systems for this Suzuki reaction. The first employed PdCl2(PPh3)2 with Na2CO3 as base and 1,4-dioxane as solvent; regarding the economically more attractive nickel, Ni(NO3)2.6H2O/PPh3 was identified as an optimal pre-catalyst, with K3PO4 as base and acetonitrile as solvent. In the Ni-catalyzed reaction, boronic acid 26 performed better than the corresponding boronate ester. The yield of the Pd-catalyzed reaction was 60%, whereas the Ni-catalyzed reaction provided 27 in 79% yield. Mild removal of the THP-protecting group then provided 24. Regarding purification after the Suzuki reaction, residual Ni was easily removed through an aqueous ammonia wash followed by crystallization, while the removal of residual Pd demanded expensive scavengers and a large volume of solvents. The easy removal of nickel traces compensated for the high loadings of its pre-catalyst (30 mol%); Pd loadings were lower (4.5 mol%), however the lower yield and difficult purification impaired the use of this noble metal.
Hicks et al. reported the synthesis of 28 (Scheme 9), a molecule connected to PI3K and AKT/PKB signalling pathway that inhibits cell proliferation and tumour growth.3131 Hicks, F.; Hou, Y.; Langston, M.; McCarron, A.; O'Brien, E.; Ito, T.; Ma, C.; Matthews, C.; O'Bryan, C.; Provencal, D.; Zhao, Y.; Huang, J.; Yang, Q.; Heyang, L.; Johnson, M.; Sitang, Y.; Yuqiang, L.; Org. Process Res. Dev.2013, 17 , 829. Initially, Suzuki coupling of 29 with 30 was performed in the presence of Na2CO3, but attempts to decrease the initial loading (3 mol%) of the palladium pre-catalyst (see Scheme 9) with this base resulted in incomplete conversions. Surprisingly, substitution of sodium carbonate by potassium phosphate allowed an impressive decrease of the Pd loading to 0.2 mol%; nonetheless, careful removal of palladium impurities was necessary, and after some testing with L-cysteine, an optimal procedure was disclosed in which the desired Suzuki product was isolated as 1:1 N,N-dimethylacetamide solvate 31 and redissolved in DMF, with Pd being removed by means of a scavenger supported on silica. Thereafter, ethanol was added as an anti-solvent for crystallization, providing 28 with palladium amounts lower than 1 ppm.
A process chemistry development was performed for the synthesis of CEP-32215 (32) (Scheme 10), an antagonist of the histamine H3 receptor;3232 Wang, Y.; Przyuski, K.; Roemmele, R. C.; Hudkins, R. L.; Bakale, R. P.; Org. Process Res. Dev.2013, 17 , 846. its antagonists can be used in a variety of central nervous system (CNS) disorders associated with attention and cognitive deficits (wakefulness, attention-deficit hyperactivity disorder, Alzheimer's disease, mild cognitive impairment and schizophrenia). The synthesis of this compound employed a curious Suzuki-type reaction of acid chloride 33 and arylboronic acid 34: this boronic acid was used in its crude form, since it was more reactive than the chromatography-purified product. The authors attributed this remarkable behaviour to the fact that, in its crude form, the boronic acid was more soluble in toluene than in its purified crystalline version. A screening of different bases, solvents, catalysts and temperatures led to the identification of PdCl2(PPh3)2 as an optimal pre-catalyst and cesium carbonate as the most effective base. Toluene proved to be the best solvent, and a small amount of water was necessary in order to solubilize the cesium carbonate. Additional ligands generally impaired the reaction. Therefore, ketone 35 was obtained in 75% yield.
Hong et al. developed a scalable synthesis of a Bruton's tyrosine kinase (BTK) inhibitor (36), an important cell signalling enzyme involved in early B cell development and mature B cell activation and survival.3333 Hong, J.-B.; Davidson, J. P.; Jin, Q.; Lee, G. R.; Matchett, M.; O'Brien, E.; Welch, M.; Bingenheimer, B.; Sarma, K.; Org. Process Res. Dev.2014, 18 , 228. These cells play a key role in autoimmune and inflammatory diseases. In the first approach for this compound, a Suzuki reaction between aryl chloride 37 and boronate ester 38 was applied in a late synthetic stage (Scheme 11).
Even though this reaction was successful in providing the desired product 39, up to 20% of impurities (40) arising from condensation of the aldehyde moiety of 39 were detected by high performance liquid chromatography (HPLC) when the reaction was carried out for longer times (as it is commonly the case in large scale synthesis). Therefore, a new strategy for this final coupling was necessary. An initial attempt was performed by protection of the free nitrogen of 38 with the Boc group, thus rendering the pyridinone ring of 39 less nucleophilic: indeed, a cyclization like that observed in 40 was avoided after the Suzuki reaction; nonetheless, conditions for removal of the Boc group cleaved the morpholine moiety as well. Thus, instead of aldehyde 39, the authors envisioned a Suzuki coupling between alcohol 41 and boron pinacolate38. However, this change in the electronic nature of the aryl halide coupling partner demanded re-optimisation of the Suzuki reaction, and then tricyclohexylphosphine and Pd(dba)2 were identified as the optimal ligand and pre-catalyst, respectively. The solvent could be switched from DMF to 2-MeTHF with no yield losses. Once the Suzuki reaction had been performed, palladium was removed by treatment with activated charcoal, and 36 was obtained in 83% yield and 99% purity as judged by HPLC (Scheme 12).
Kallman et al. reported a synthesis of LY2801653 (42), a type-II ATP competitive inhibitor of mesenchymal epithelial transition factor (MET), which is a member of the receptor tyrosine kinase family.3434 Kallman, N. J.; Liu, C.; Yates, M. H.; Linder, R. J.; Ruble, J. C.; Kogut, E. F.; Patterson, L. E.; Laird, D. L. T.; Hansen, M. M.; Org. Process Res. Dev.2014, 18 , 501. The MET pathway has been associated with the progression of some tumours and MET overexpression has been observed in many, including colon, renal, lung, head and neck squamous cell carcinoma and gastric cancer. In a first study, Suzuki coupling between bromide 43 and protected pinacolboron44 furnished a mixture of 45 and 46, showing that the Boc protecting group was not stable under the employed Suzuki conditions. Moreover, 46 significantly poisoned the Pd catalyst (Scheme 13).
The conditions for the Suzuki reaction were screened again, and with THF as solvent and Boc2O as additive, the yield of 45 could be improved to 75%. Nonetheless, further manipulation of 45 led to cleavage of the Boc group and gave rise to dangerous, mutagenic impurities. As a result, the authors decided to investigate a different approach to the synthesis, performing a Suzuki reaction with aryl bromide47, which is very close to the final product, thus minimizing the manipulation of the Boc-containing product. Removal of this protecting group could then be performed in basic media, which prevented the amide linkage of 48 from being cleaved (Scheme 14).
Tetrasubstituted tiophenes 49-51 were identified by Huang et al. as highly promising candidates for the selective inhibition of the PI3K receptor, a property that is associated with the treatment of some types of cancer (Figure 2).3535 Huang, Q.; Richardson, P. F.; Sach, N. W.; Zhu, J.; Liu, K. K. C.; Smith, G. L.; Bowles, D. M.; Org. Process Res. Dev.2011, 15 , 556.
The initial routes to these compounds displayed no cross-coupling reactions, with the benzene rings of 49-51 being introduced in the first step of the synthesis, therefore making a common intermediate impossible. For the scale-up, a synthesis featuring a Suzuki reaction between substituted iodothiophenes 51 and the corresponding arylboronic acids was envisioned (Scheme 15); however, some challenges were anticipated, such as the poor reactivity of iodothiophenes in the coupling step and the sterically demanding nature of 51. The Suzuki cross-coupling reaction indeed proved itself to be difficult; for example, reaction with 51a furnished only low to moderate yields of the desired product after approximately 100 conditions being tested, with large amounts of deiodinated by-products being observed. The authors believe that the poor solubility of 52a impaired the Suzuki reaction, and an additional drawback was the fact that the corresponding cross-coupling product was also poorly soluble, therefore making purification difficult. On the other hand, when the corresponding ethyl ester 52b was investigated, very satisfactory conditions could be identified after the employment of a high-throughput experimentation methodology, with catalysts [Pd(tBu3P)4, Pd(PPh3)4, PdCl2(dppf)], bases (CsF, diisopropylethylamine, K2CO3, K3PO4) and solvents (toluene, dioxane, ethanol, water) being combined under 200 different conditions. After data analysis, the conditions displayed in Scheme 15 were identified, with the three desired coupling products being obtained in good to excellent yields after purification by crystallization.
Walker et al. developed a large-scale synthesis of a GPR40 receptor agonist, a class of compounds that is being currently investigated as possible therapeutic agents for the treatment of type 2 diabetes.3636 Walker, S. D.; Borths, C. J.; DiVirgilio, E.; Huang, L.; Liu, P.; Morrison, H.; Sugi, K.; Tanaka, M.; Woo, J. C. S.; Faul, M. M.; Org. Process Res. Dev.2011, 15 , 570. The target compound, AMG 837 (53), was obtained in a convergent way by joining Suzuki cross-coupling derived biphenyl54 with enantiomerically pure β-alkynylcarboxylic acid 55 (Scheme 16).
Retrosynthetic plan for the final steps of the Walker et al.36 large-scale synthesis of AMG 837 (53).
The first synthetic approach to 53 was based on a Suzuki reaction between aryl bromide 55 and arylboronic acid56. A catalytic system containing palladium supported on charcoal, sodium carbonate and diisopropylamine/water as solvents was employed (Scheme 17), providing biphenyl 57 in 91% yield. In turn, compound 53 was obtained by borane-mediated reduction of 57 and bromination of the resulting benzylic alcohol.
Further investigation, however, was carried out in order to shorten the preparation of 54 and to avoid the reduction step. Thus, the authors were able to couple economically more attractive aryl chloride 59 with arylboronic acid 60 employing 1 mol% of Pd2(dba)3 as a pre-catalyst and 2.2 mol% of tricyclohexylphosphine as a ligand in a mixture of water and THF, 61 was obtained in an excellent yield of 95% (Scheme 18). Hydroxyl displacement with SOBr2 then furnished biphenyl 54 in an overall yield of 86% starting from the aryl chloride 59.
En route to producing large quantities of Crizotinib (62), a c-Met/anaplastic lymphoma kinase (ALK) inhibitor in advanced clinical tests, De Koning et al. employed a Suzuki reaction in order to generate an advanced intermediate.3737 De Koning, P. D.; McAndrew, D.; Moore, R.; Moses, I. B.; Boyles, D. C.; Kissick, K.; Stanchina, C. L.; Cuthbertson, T.; Kamatani, A.; Rahman, L.; Rodriguez, R.; Urbina, A.; Sandoval, A.; Rose, P. R.; Org. Process Res. Dev.2011, 15 , 1018. However, before describing the large-scale synthesis itself, it is worth noting some aspects of the initial medicinal chemistry screening: compounds with the basic structure 63 were obtained through a sequence of protection, palladium-catalyzed borylation, deprotection and Suzuki reactions (Scheme 19) in order to allow for late-stage functionalization with substituted bromopyrazoles.
Then, once crizotinib (and its R isomer) had been identified as the most promising candidate, the Suzuki cross-coupling was performed in an inverse way: the pyrazol moiety was employed as its boron pinacolate derivative (64, Scheme 20). Even though 64 was obtained in a moderate 40% yield, its Suzuki reaction furnished the desired product in excellent quantities, thus allowing a better use of the valuable chiral intermediate 65.
The large-scale synthesis demanded further modifications: the preparation of multi-kilogram amounts of imidazole 64 presented high levels of dimerization; therefore, the palladium-catalyzed borylation was abandoned in favour of a Knochel-type procedure.3838 Baron, O.; Knochel, P.; Angew. Chem., Int. Ed.2005, 44 , 3133. The Suzuki cross-coupling was performed in a different solvent system: in order to avoid the undesirable DME, toluene and water were employed together with the phase-transfer catalyst tetrabutylammonium bromide (TBAB). This new procedure allowed the catalyst loading to be reduced to 0.9 mol% with a significant simplification of the purification procedure, once the cross-coupling product was soluble in toluene and hence easily separated from the water layer (Scheme 21).
The β2-adrenoreceptor PF-00610355 (66, Scheme 22), recently found to be effective in the treatment of respiratory diseases, was synthesized on a large scale by Thomsonet al. .3939 De Koning, P. D.; Castro, N.; Gladwell, I. R.; Morrison, N. A.; Moses, I. B.; Panesar, M. S.; Pettman, A. J.; Thomson, N. M.; Org. Process Res. Dev.2011, 15 , 1256. The biaryl moiety of the target compound was assembled by means of Suzuki cross-coupling, which delivered 67 in good yield with a very low charge of palladium pre-catalyst (Scheme 22). Interestingly, in this reaction, 3% of the aryl bromide 68 remained unconverted after 2 h of reaction, demanding a second loading of the same initial amount of the pre-catalyst in order to achieve full conversion.
Bowles et al. developed a large-scale preparation of PF-03052334-02 (69), a pyrazole displaying reduced risk of musculoskeletal side effects in the treatment of coronary diseases (Scheme 23).4040 Bowles, D. M.; Boyles, D. C.; Choi, C.; Pfefferkorn, J. A.; Schuyler, S.; Hessler, E. J.; Org. Process Res. Dev.2011, 15 , 148. For coupling of the diol-containing side chain, the authors envisioned a Suzuki cross-coupling between triflate 70 and vinylboronic acid71; however, due to stability issues surrounding 71, this reaction failed to deliver the desired product. Therefore, an alternative was found in which 70 was coupled with styrenyl boronic acid 72, delivering the Suzuki product 73 in excellent yield. After that, 73 was submitted to ozonolysis, providing aldehyde 74 in 76% yield. The side chain was then installed by a Wittig reaction, whose product 75, after chemo- and regioselective reductions and deprotections, afforded 69.
Regarding bench-scale processes, Patel and Barret achieved a total synthesis of AT13387 (76), a molecule that is capable of interacting with chaperones that are essential for the survival of cancer cells.4141 Patel, B. H.; Barrett, A. G. M.; J. Org. Chem.2012, 77 , 11296. In their synthesis, the piperazino-iso-indoline 77 was assembled by a sp3-sp2 Suzuki cross-coupling reaction employing the Molander protocol (Scheme 24).4242 Molander, G. A.; Gormisky, P. E.; Sandrock, D. L.; J. Org. Chem.2008, 73 , 2052.
Donohoe et al. achieved a total synthesis of the antitumor antibiotic (±)-streptonigrin (78), employing a variety of transition metal-catalysed reactions.4343 Donohoe, T. J.; Jones, C. R.; Kornahrens, A. F.; Barbosa, L. C. A.; Walport, L. J.; Tatton, M. R.; O'Hagan, M.; Rathi, A. H.; Baker, D. B.; J. Org. Chem.2013, 78 , 12338. While out of the scope of this text, it is worth noting that pentafunctionalized pyridine 79 was obtained by means of a challenging ring-closing metathesis reaction. Then, for constructing the AB-C ring system, the authors expected the triflate moiety of 79 to be more reactive towards Pd(0) oxidative addition in comparison to the bromide, mostly due to the increased reactivity of cross-coupling active substituents at the 2-position of pyridine (Scheme 25).
Thus, Stille reaction of pyridine 79 with stannylquinoline 80 provided the AB-C core of the desired product in 77% yield. Thereafter, Suzuki reaction with activation of the bromine substituent in 81 provided the tetracyclic compound 82 in 74% yield. Once the C-D ring system is configurationally stable, giving rise to atropoisomers, the authors also tried to perform the above Suzuki cross-coupling in an enantioselective fashion, with the best ratio between yield and enantioselectivity being achieved by means of the phosphino hydrazone 83 as a ligand (Figure 3) (65% yield and 42% ee).
3. Negishi Reaction
The Negishi reaction is the coupling between an aryl (or vinyl) halide and an organozinc compound.4444 Negishi, E.; Acc. Chem. Res.1982, 15 , 340.,4545 Negishi, E.-I.; Bull. Chem. Soc. Jpn.2007, 80 , 233. This coupling presents a greater tolerance towards sensitive groups like esters or amides when compared to organomagnesium or organolithium-based reactions; moreover, the enhanced reactivity of organozinc compounds allows this reaction to be performed at or even below room temperature, while boron or tin coupling partners demand harsher conditions.
In 2013, a Negishi coupling was employed as one of the key steps in a new synthetic approach to BMS-599793 (84), an HIV entry inhibitor licensed from Bristol-Myers Squibb (BMS) by the International Partnership for Microbicides aiming towards its further development as a topical microbicide candidate for use in underdeveloped countries.4646 Pikul, S.; Cheng, H.; Cheng, A.; Huang, C. D.; Ke, A.; Kuo, L.; Thompson, A.; Wilder, S.; Org. Process Res. Dev.2013, 17 , 907. While the original medicinal chemistry route employed a Stille coupling as the last step of the sequence, this new synthetic approach anticipates the metal-catalyzed C-C bond forming reaction using a Negishi coupling as the first step, which eliminated the inconvenience of high levels of toxic tin residues previously observed in the final product. The coupling between diarylzinc reagent 85 and azaindol 86 led to adduct 87, obtained in 41 to 59% yield for several batches of 400 g each. A single experiment carried out at a 1.5 kg scale afforded 87 in 54% yield and 99.6% HPLC purity (Scheme 26).
Acylation of the adduct's azaindole moiety with methyl chlorooxalate followed by ester hydrolysis and amidation afforded the final product which, however, still exhibited a high residual content of palladium (16 ppm) and zinc (100 ppm), requiring additional purification steps. At the end of the synthetic sequence, product treatment with activated charcoal followed by several washes, a precipitation step and polymorphic conversions led to the final active pharmaceutical ingredient (API) with Pd and Zn contents of only 1 ppm each.
A Negishi reaction was also used in a multi-gram synthesis of compound 88, a CHRT2 receptor antagonist with good pharmacological profile that was previously identified by Roche.4747 Shu, L.; Wang, P.; Gu, C.; Liu, W.; Alabanza, L. M.; Zhang, Y.; Org. Process Res. Dev.2013, 17 , 651. Since it is believed that CHTR2 plays a pro-inflammatory role in allergic processes, its antagonists are viewed as potential new drugs for the treatment of asthma, allergic rhinitis, chronic obstructive pulmonary disease (COPD) and atopic dermatitis, among other diseases. En route to 88, the Negishi coupling between benzylzinc reagent 89 and aryl triflate 90 represented a major challenge. According to the previously developed medicinal chemistry synthetic route, the arylzinc preparation required a highly exothermic zinc activation process and the subsequent coupling step employed very high catalyst loadings, both of which are serious drawbacks for the scaling-up of the synthesis (Scheme 27).
During the process development, a traditional zinc activation approach was found to be effective, and by using trimethylchlorosilane in DMF, 237 g of zinc dust could be activated with only a 5 ºC temperature increase over a 30 min period.
Regarding the proper Negishi coupling step, in a first moment, the authors were able to perform a 20-fold reduction in both palladium and phosphine quantities; however, the high cost of SPhos ligand was still prohibitive for large-scale production, even at 1 mol% loading. Fortunately, the inexpensive PdCl2(PPh3)2 complex was equally effective when employed as the sole catalyst at 0.5 mol% loading. Due to safety reasons associated with transferring a high reactive zinc reagent, the coupling reaction was performed at the same pot of zincate formation by the sequential addition of the Pd source and aryl triflate followed by heating to 60-65 ºC, which initiated the reaction. Without further heating, a temperature increase of up to 30 ºC in a 10 min period was observed for batches involving 500 g of trifate 90, which should be bypassed for further scale-up. Filtration of the reaction crude trough Celite and treatment with N-acetyl-L-cysteine followed by crystallization afforded the coupling product 91 in 95% yield and > 99% HPLC purity. The final hydrolysis step afforded the desired API, which displayed well-controlled levels of residual palladium and zinc (6 and 20 ppm, respectively).
4. Sonogashira Reactions
The construction of a new bond between sp22 Beletskaya, I. P.; Cheprakov, A. V.; Chem. Rev.2000, 100 , 3009.- and sp-hybridized carbons is known as the Sonogashira reaction,4848 Sonogashira, K.; Tohda, Y.; Hagihara, N.; Tetrahedron Lett.1975, 16 , 4467. and it is nowadays a widely employed methodology for the construction of arylacetylenes.33 Chinchilla, R.; Najera, C.; Chem. Soc. Rev.2011, 40 , 5084.,4949 Bakherad, M.; Appl. Organomet. Chem.2013, 27 , 125.,5050 Chinchilla, R.; Nájera, C.; Chem. Rev.2007, 107 , 874. For example, a Sonogashira coupling was employed by the research and development group of Kyowa Hakko Kirin in a new and concise synthetic route for olopatadine hydrochloride (92), a commercial anti-allergic drug that was previously developed by the same company.5151 Nishimura, K.; Kinugawa, M.; Org. Process Res. Dev.2012, 16 , 225.
The reported synthesis goes through the Sonogashira reaction between the easy accessible aryl halide 93 and alkyne 94 leading to adduct 95 in 94% yield. This adduct is then subjected to a second metal-catalyzed transformation, a stereospecific palladium-catalyzed intramolecular cyclization, whose optimum conditions were identified based on an elegant and comprehensive Design of Experiments (DoE) investigation to provide 96 (Scheme 28).
Elaboration of the cyclization product 96 through aminomethylation and ester hydrolysis followed by acid work-up completes the synthesis of the final target. Although the presented synthetic route is very promising and concise, providing olopatadine hydrochloride in 54% overall yield for 6 steps from commercially available materials, it has so far been reported only on a laboratory scale (5 g for the Sonogashira coupling and 200 mg for the cyclization step).
In 2012, in order to obtain necessary quantities for preclinical and phase 1 clinical studies, investigators at Wyeth Research reported the process implementation for the multi-kilogram preparation of GRN-529 (97), a highly selective mGluR5 negative allosteric modulator that was previously identified by the same company.5252 Sperry, J. B.; Farr, R. M.; Levent, M.; Ghosh, M.; Hoagland, S. M.; Varsolona, R. J.; Sutherland, K.; Org. Process Res. Dev.2012, 16 , 1854. The authors opted for the development of the synthetic route initially presented by the medicinal chemistry group, which featured a Sonogashira coupling as one of the key steps. Despite the good yield observed for this transformation, the conditions originally employed were not amenable for the scale-up of the reaction, requiring high loadings of metallic catalysts and drastic reaction conditions, as well as chromatographic purification of the product. During the developmental work, process friendly conditions were identified for the effective coupling between aryl halide 98 and alkyne 99, resulting in at least 10-fold reduction of metal catalyst loadings and considerably lower reaction temperatures (Scheme 29).
A purification process involving the treatment of the crude reaction with an aqueous cysteine and ammonia solution followed by crystallization of the product afforded the coupling adduct 100 with high purity, acceptable levels of residual palladium and copper, and an even higher yield (86%). Elaboration of the coupling product 100 through ester hydrolysis followed by amidation furnished the final API, which was further purified via crystallization, leading to even lower residual levels of palladium and copper (< 1 and 15 ppm, respectively). This process was successfully employed in the preparation of 5.5 kg of 97.
Process chemistry researchers from Merck recently employed Heck and Sonogashira reactions in the multi-gram preparation of the macrocyclic compound MK-1220 (101),5353 Song, Z. J.; Tellers, D. M.; Dormer, P. G.; Zewge, D.; Janey, J. M.; Nolting, A.; Steinhuebel, D.; Oliver, S.; Devine, P. N.; Tschaen, D. M.; Org. Process Res. Dev.2014, 18 , 423. a potent hepatitis C virus (HCV) protease inhibitor with good therapeutic profile which has been evaluated as a potential new drug for the treatment of hepatitis C.5454 Rudd, M. T.; McCauley, J. A.; Butcher, J. W.; Romano, J. J.; McIntyre, C. J.; Nguyen, K. T.; Gilbert, K. F.; Bush, K. J.; Holloway, M. K.; Swestock, J.; Wan, B.-L.; Carroll, S. S.; DiMuzio, J. M.; Graham, D. J.; Ludmerer, S. W.; Stahlhut, M. W.; Fandozzi, C. M.; Trainor, N.; Olsen, D. B.; Vacca, J. P.; Liverton, N. J.; ACS Med. Chem. Lett.2011, 2 , 207.
As an early synthetic step, a Heck reaction was used to introduce the nitrogen and two carbon atoms of the isoquinoline moiety present in the final target. The presence of a bulky substituent in the olefin 102 allowed the achievement of a 90:10 regioselectivity favouring the arylation of the terminal carbon, leading to adduct 103 in 76% isolated yield (Scheme 30).
A Sonogashira reaction was employed at a later stage of the synthesis, allowing the coupling of the advanced fragments 104 and 105 in 90% yield. The reaction conditions for this coupling were chosen based on a high throughput screening (HTS) study, which evaluated, among other factors, the use of 12 distinct ligands. Among them, only PtBu3.HBF4 led to a clean reaction and satisfactory conversion. The triple bond reduction of the coupling adduct 106 with concomitant removal of carboxybenzyl (CBz) protecting group led directly to the substrate for the key macrocyclization step (107). The side chain installation in 107 completed the total synthesis of MK-1220. According to the authors, this synthetic sequence could be successfully scaled-up to produce kilograms of the final API.
The Sonogashira reaction has also recently been used in the synthesis of Filibuvir (108), a potential drug for hepatitis C treatment, which acts as an inhibitor of RNA polymerase and has been evaluated by clinical and toxicological studies.5555 Singer, R. A.; Ragan, J. A.; Bowles, P.; Chisowa, E.; Conway, B. G.; Cordi, E. M.; Leeman, K. R.; Letendre, L. J.; Sieser, J. E.; Sluggett, G. W.; Stanchina, C. L.; Strohmeyer, H.; Blunt, J.; Taylor, S.; Byrne, C.; Lynch, D.; Mullane, S.; O'Sullivan, M. M.; Whelan, M.; Org. Process Res. Dev.2014, 18 , 26.
The coupling between the alkyne 109 and aryl bromide 110 (Scheme 31), employed to introduce the pyridine moiety present in the final target, required the careful exclusion of oxygen from the reaction medium and addition of the palladium source prior to the copper catalyst, taking special care to avoid the formation of by-products through the oxidative coupling of the alkyne coupling partner (Scheme 31).
The coupling adduct 111 was then subjected to an acylation reaction followed by reduction of the triple bond to afford 112. One of the drawbacks initially observed in this sequence was the high catalyst loading required for the hydrogenation step, which was attributed to poisoning of the metallic catalyst by residues of copper and phosphine derived from the initial Sonogashira coupling. Thus, previous purification of the acylated coupling product by washes with aqueous ethylenediaminetetraacetic acid (EDTA) followed by filtration through activated charcoal was necessary. This sequence of reactions was carried out on a pilot scale, producing 11.95 kg of the advanced intermediate 112.
It should be mentioned that alkyne 109 is prepared by an aldol addition to the ketone 113 which, in turn, may also be prepared through a Sonogashira-type reaction involving the acid chloride 114 followed by desilylation. Although the authors present alternative and possibly more suitable synthetic routes for the preparation of this alkynyl ketone, the procedure based on the coupling between 109 and 110 could be successfully employed in the preparation of 100 kg of this material.
5. Stille Cross-Coupling
The coupling between an organic electrophile and an organotin compound is known as the Stille reaction.5656 Stille, J. K.; Angew. Chem., Int. Ed.1986, 25 , 508. It may be employed for the construction of vinyl-vinyl, aryl-vinyl or aryl-aryl bonds. Although the toxicity and difficult removal of tin compounds, which can present problems on an industrial scale, the Stille coupling reaction presents similar advantages to the Suzuki reaction (accessibility of organostannanes, air and moisture stability, tolerance towards most functional groups) and is usually superior for the synthesis of complex molecules, affording an alternative for the synthesis in medicinal chemistry scale. For instance, a Pd-catalyzed intramolecular Stille cross-coupling was applied as a key macrolide formation in a total synthesis of the iejimalide B (115) (Scheme 32), an anticancer compound isolated from the tunicate species Eudistoma cf. rigida, which is native to the coral reefs in Japan.5757 Chen, Q.; Schweitzer, D.; Kane, J.; Davisson, V. J.; Helquist, P.; J. Org. Chem.2011, 76 , 5157. A system based on Pd2(dba)3.CH3Cl and Ph3As provided cyclic advanced intermediate 116 with 72% yield. However, it must be mentioned that 60 mol% of palladium was employed in the reaction. The Stille coupling was chosen instead of a Suzuki reaction for this macrocyclization due to the fact that hydroboration of the alkyne intermediate 117 proved difficult to generate the Suzuki partner while the hydrostannylation afforded the intermediate stannane118 with 75% yield. This result was attributed to the high functional group tolerance of the hydrostannylation in comparison with hydroboration.
In 2013, the total synthesis of the HIV-1 integrase inhibitor 119 was described in a nine step route (Scheme 33).5858 Okello, M.; Nishonov, M.; Singh, P.; Mishra, S.; Mangu, N.; Seo, B.; Gund, M.; Nair, V.; Org. Biomol. Chem.2013, 11 , 7852. The authors employed the bromopyridine120 as starting material and the target compound was obtained with 18% overall yield. In the insertion of a side chain in the pyridinone central ring, the Stille cross-coupling of a vinyl stannane was applied and led to the product 121 with 83% yield after 1 h of reaction in DMF. A large amount (10 mol%) of the pre-catalyst PdCl2(PPh3)2 in combination with a 20% excess of the tin reagent was necessary to provide high yields. It must be mentioned that the iodinated substrate was also applied in the cross-coupling; however, according to the authors, the bromo derivative was preferred due to a simpler chromatographic purification.
Total synthesis of the HIV-1 integrase inhibitor 119 using a Stille reaction to append the carbonylated side-chain to the central pyridine ring.
6. Kumada Cross-Coupling
The coupling of organomagnesium compounds and organic electrophiles is known as the Kumada reaction and is normally catalyzed by palladium or nickel complexes.5959 Tamao, K.; Sumitani, K.; Kumada, M.; J. Am. Chem. Soc.1972, 94 , 4374. ST1535 (122) is a highly selective adenosine A2A receptor ligand antagonist with good pharmacological properties, which might be considered a potential new drug for the treatment of Parkinson's disease.6060 Bartoccini, F.; Piersanti, G.; Armaroli, S.; Cerri, A.; Cabri, W.; Tetrahedron Lett.2014, 55 , 1376. Previous approaches for its synthesis explored Stille or Suzuki couplings for the installation of the alkyl side chain present in the final target.6161 Minetti, P.; Tinti, M. O.; Carminati, P.; Castorina, M.; Di Cesare, M. A.; Di Serio, S.; Gallo, G.; Ghirardi, O.; Giorgi, F.; Giorgi, L.; Piersanti, G.; Bartoccini, F.; Tarzia, G.; J. Med. Chem.2005, 48 , 6887.,6262 Bartoccini, F.; Cabri, W.; Celona, D.; Minetti, P.; Piersanti, G.; Tarzia, G.; J. Org. Chem.2010, 75 , 5398. Despite the good yields observed, both approaches suffered from major drawbacks such as drastic reaction conditions, low turnover number and frequency for the palladium catalysts employed, the need to prepare organoboron or tin reagents and the toxicity of starting materials and by-products in the special case of Stille reaction.
In this context, in 2014, the authors described a new approach exploring the Kumada coupling of a Grignard reagent, one of the cheapest organometallic reagents available, catalyzed by Fe(acac)3, where acac = acetylacetonate, which is also a low-cost and easily removable catalyst. Besides the advantages regarding the cost of reagents and catalysts, the new procedure also generates less waste, due to the intrinsic higher atom-economy and also by not requiring an excess base, as seen for the Suzuki coupling approach. This procedure was successfully employed in the preparation of 38.5 g of 123, which could be converted to 122 through bromination and bromine displacement with triazole followed by benzyl protecting group removal, as previously described in the literature (Scheme 34).
7. Heck Reactions
The palladium-catalyzed olefination of a sp22 Beletskaya, I. P.; Cheprakov, A. V.; Chem. Rev.2000, 100 , 3009. or benzylic carbon attached to a (pseudo)halogen is known as the Heck reaction.22 Beletskaya, I. P.; Cheprakov, A. V.; Chem. Rev.2000, 100 , 3009.,6363 Heck, R. F.; Nolley, J. P.; J. Org. Chem.1972, 37 , 2320. It is a powerful tool, mainly used for the synthesis of vinylarenes, and it has also been employed for the construction of conjugated double bonds. The widespread application of this reaction can be illustrated by numerous examples in both academia small-scale6464 De Vries, J. G.; Can. J. Chem.2001, 79 , 1086. and industrial syntheses.55 Torborg, C.; Beller, M.; Adv. Synth. Catal.2009, 351 , 3027. As an example, in 2011, a idebenone (124) total synthesis based on a Heck reaction was described (Scheme 35).6565 Tsoukala, A.; Bjørsvik, H.-R.; Org. Process Res. Dev.2011, 15 , 673. This compound, initially designed for the treatment of Alzheimer's and Parkinson's diseases, presented a plethora of other interesting activities, such as free radical scavenging and action against some muscular illnesses. The key step in the synthesis was the coupling of 2-bromo-3,4,5-trimethoxy-1-methylbenzene (125) with dec-9-en-1-ol affording products 126. Under non-optimized conditions (Pd(OAc)2, PPh3, Et3N, 120 ºC), a mixture composed of 60% linear olefins 126 and 15% of the undesired branched product 127 was obtained after three days of reaction. Therefore, the conditions were optimized, allowing the preparation of 126 in 67% yield with no detection of 127 after only 30 min of reaction employing DMF, Pd(PPh3)4, iPr2NEt under microwave heating. To conclude the synthesis, the Heck adducts were submitted to hydroxyl protection/deprotection, hydrogenation, and ring oxidation. After these reactions, idebenone was obtained with 20% overall yield over 6 steps.
Synthesis of idebenone (124) based on Heck reaction of 2-bromo-3,4,5-trimethoxy-1-methylbenzene with dec-9-en-1-ol under microwave irradiation.
A similar strategy to link an aryl and an alkyl group by means of a Heck reaction was applied in 2013 in the synthesis of ginkgolic acid (13:0) (128), a tyrosinase inhibitor, as shown in Scheme 36.6666 Fu, Y.; Hong, S.; Li, D.; Liu, S.; J. Agric. Food Chem.2013, 61 , 5347. Benzoic acid 88 was used as starting material in order to obtain the triflate 130. This intermediate and the 1-tridecene were employed as coupling partners in the Heck reaction to obtain 131. The system used was based on PdCl2(dppf) and K2CO3, affording the coupling product in 78% yield after 12 h with 3.2 mol% of palladium. An alternative pathway to link the alkyl and aryl fragments was also tested: the coupling was performed in the presence of 9-borabicyclo(3,3,1)-nonane (9-BBN) to generate an alkylborane in situ from olefin 132 and achieve Suzuki coupling. However, this methodology was not productive. Once the Heck reaction was performed, 128 was obtained with 34% overall yield after the reduction of the double bond and the hydrolysis of the acetal protecting group.
An intramolecular Heck-based cyclization was used as a key step for commendable synthesis of the antihistaminic drug olopatadine (133) and its trans isomer (134).6767 Bosch, J.; Bachs, J.; Gómez, A. M.; Griera, R.; Écija, M.; Amat, M.; J. Org. Chem.2012, 77 , 6340. Besides the Heck reaction, another vital step in this route was a stereoselective Wittig olefination using a non-stabilized phosphorus ylide that afforded the olefins 135 and 136 (E:Z ratio = 9:1 for 135, for instance). Concerning the Heck reaction, Pd(OAc)2, K2CO3, and NBu4Cl (TBAC) were allowed to react with 135 and 136 at 60 ºC during 24 h, providing the cyclic adducts 137 and 138 with reasonable 60% and 55% yields, respectively. However, it is important to note that in catalytic terms, the results were not encouraging, considering that 20 mol% of palladium was used and a disappointing turnover number (TON) of 3 was observed (Scheme 37).
In relation to the stereochemistry of the Heck products, the above results were not surprising since they were consistent with a syn-insertion of the arylpalladium intermediate (provided by oxidative addition step) at the olefinic moiety followed by a syn β-elimination that afforded the product with the ascribed stereochemistry. Finally, with the cyclic products in hands, the syntheses were completed by alkaline hydrolysis of methyl esters that afforded the target olapatadine and trans-olapatadine.
Two Heck-based strategies were applied in the synthesis of caffeine-styryl compounds (139) with dual A2A antagonist/MAO-B inhibition properties and potential application in Parkinson's disease (Scheme 38).6868 Rivara, S.; Piersanti, G.; Bartoccini, F.; Diamantini, G.; Pala, D.; Riccioni, T.; Stasi, M. A.; Cabri, W.; Borsini, F.; Mor, M.; Tarzia, G.; Minetti, P.; J. Med. Chem.2013, 56 , 1247. In the first approach, the bromide 140 was submitted to a Suzuki cross-coupling with vinylboronic acid pinacol ester affording the vinyl-caffeine intermediate 141 in 67% yield. This intermediate was used as substrate in the Heck reaction with various aryl bromides in a system composed of Pd(OAc)2, P(o-tol)3, Et3N and DMF. Accordingly, the caffeine-styryl analogues 139 were obtained with yields ranging from 30 to 84% in 2 h of reaction at 80 ºC, with 10 mol% of palladium. In the second approach, the organic bromide 140 was used directly under Heck conditions in combination with styrene derivatives, DMF, NBu4Br (TBAB) and Pd(OAc)2. With this strategy, the target compounds 139 obtained with 20 to 86% yield in 20 h of reaction with 5 mol% of catalytic precursor. In terms of biological activities, the compound that exhibited the best results was 142, the structure of which is shown at the bottom of Scheme 38.
A facile synthesis of styryl analogues of piperidine alkaloids was developed recently using N-Boc-piperidine as starting material (Scheme 39).6969 Kankala, S.; Kankala, R. K.; Balaboina, R.; Thirukovela, N. S.; Vadde, R.; Vasam, C. S.; Bioorg. Med. Chem. Lett.2014, 24 , 1180. These alkaloids, including the (+)-caulophyllumine B (143), display high anti-cancer activity in vitro.6969 Kankala, S.; Kankala, R. K.; Balaboina, R.; Thirukovela, N. S.; Vadde, R.; Vasam, C. S.; Bioorg. Med. Chem. Lett.2014, 24 , 1180. The first step of the synthesis was an enantioselective vinylation of the starting material affording the intermediate 144 that was used as substrate for Heck reactions with aryl iodides. In order to perform this Heck reaction, palladium acetate (5 mol%) was used as a pre-catalyst, in combination with an N-heterocyclic carbene (NHC) precursor and the phase transfer reagent TBAB. Under these conditions, styryl-piperidine adducts 145 were produced in excellent yields (88-93%). Finally, the target structures 146 were obtained by the reductive removal of the Boc protective group of compounds 145. Compound 143 could be prepared after methylation of the appropriate precursor 146.
Recently, Pfizer researchers described an efficient Heck- and Migita-based multi-kilogram process for the vascular endothelial growth factor (VEGF) inhibitor axitinib (147) (Scheme 40).7070 Chekal, B. P.; Guinness, S. M.; Lillie, B. M.; McLaughlin, R. W.; Palmer, C. W.; Post, R. J.; Sieser, J. E.; Singer, R. A.; Sluggett, G. W.; Vaidyanathan, R.; Withbroe, G. J.; Org. Process Res. Dev.2014, 18 , 266. A Migita coupling was used in the first step of the synthesis in order to assemble the mercaptobenzamide moiety of 147. In the last step of the route, a Heck reaction between 148 and 2-vinyl-piridine was designed. However, the free NH of 148 impaired the reaction. Therefore, the authors decided to acylate the indazole for both protecting the NH group and rendering the oxidative addition to palladium easier. The protection was achieved in situ with Ac2O and when no more than 2% of 148 was present, the Heck reaction was started using 4 mol% of Pd(OAc)2, 4 mol% of XantPhos and six equivalents of 2-vinyl-pyridine. When the reaction was completed, 1,2-diaminopropane (DAP) was added in THF for removal of the protective group and to chelate the palladium before product crystallization. With this strategy, 29 kg of axitinib could be obtained with 50% overall yield in a six steps route.
A variation of the Heck reaction that has attracted considerable attention in recent years is the so-called Heck-Matsuda arylation, which is based on the use of aryldiazonium salts as electrophiles.7171 Kikukawa, K.; Nagira, K.; Wada, F.; Matsuda, T.; Tetrahedron1981, 37 , 31. These compounds have the advantages of lower cost and improved reactivity in comparison to more traditional electrophiles like aryl halides.1818 Taylor, J. G.; Moro, A. V.; Correia, C. R. D.; Eur. J. Org. Chem.2011, 2011 , 1403. As an example, a Heck-Matsuda based strategy via aryl-coumarins was used in the synthesis of (R)-tolterodine (149), a drug that is employed in urinary incontinence treatment.7272 Barancelli, D. A.; Salles, A. G.; Taylor, J. G.; Correia, C. R. D.; Org. Lett.2012, 14 , 6036. The coupling was employed in the first step of the route between the ortho-substituted cinnamate 150 and the 4-bromo substituted arenediazonium salt 151, using 10 mol% of Pd(OAc)2 as a pre-catalyst and CaCO3 as base. It is important to note that the target molecule tolterodine contains no bromine substituents, for this reason, after completion of the Heck reaction, the product was not isolated, being directly submitted to debromination under H2 atmosphere and subsequently isolated as 152 with 63% over two steps (Scheme 41). The employment of the bromo-substituted aryldiazonium salt instead of phenyldiazonium salt was justified by the lower yield of the Heck reaction using PhN2BF4 (only 41% yield). Nonetheless, compound 152 had already been synthesized via a Heck reaction with 77% yield without requirement of the debromination step.7373 Ulgheri, F.; Marchetti, M.; Piccolo, O.; J. Org. Chem.2007, 72 , 6056. With the desired coumarin 152 in hands, the synthesis was accomplished by a copper-catalyzed enantioselective conjugate reduction, furnishing 153, followed by reductive amination. With this methodology, (R)-tolterodine was obtained in 30% overall yield and 98% ee after four steps.
Synthesis of (R)-tolterodine based on a Heck-Matsuda reaction and enantioselective reduction of compound 152.
A Heck-Matsuda methodology was applied in the synthesis of the anti-cancer tetrahydroisoquinoline alkaloid ectenascidin 743 (154).7474 Kawagishi, F.; Toma, T.; Inui, T.; Yokoshima, S.; Fukuyama, T.; J. Am. Chem. Soc.2013, 135 , 13684. The diazonium salt156 was generated in situ by the treatment of 155 with BF3.OEt2 and tBuONO and reacted with the enamide intermediate 157 in the presence of Pd2(dba)3 (15 mol%) and NaOAc. In this way, the reaction occurred exclusively in the less hindered face of the olefin, affording 158 with regio- and stereocontrol. It is important to mention that 158 was roughly purified and employed for subsequent dihydroxylation, leading to 159 in 93% yield for the two steps in a 2.15 gram scale, which is a significant amount given the complexity of the targets (Scheme 42).
Heck-Matsuda reaction applied in the synthesis of 158, an intermediate in the synthesis of 154.
Heck-Matsuda reactions of substituted allylamines were employed as key steps in the synthesis of the bioactive compounds abamine-SG (160), abamine (161) and naftifine (162) (Scheme 43).7575 Prediger, P. C.; Barbosa, L. S. F.; Génisson, Y.; Correia, C. R. D.; J. Org. Chem.2011, 76 , 7737. Concerning the abamines, the reaction between the allylamine 163 and the corresponding diazonium salt in the presence of 10 mol% of Pd2(dba)3 afforded the adduct 164 with 84% yield and no detection of the undesired branched product. With the coupling product in hands, the syntheses were accomplished with 55% and 53% overall yields. With respect to naftifine, 4 mol% of the pre-catalyst were employed in the coupling of PhN2BF4 with the allylamine 165, leading to the intermediate 166 with 85% yield and total regio- and stereocontrol. The authors believe that this excellent selectivity arises from a substrate-directed reaction: in complexing with both the olefin and carbonyl moieties of the substrate, the cationic arylpalladium intermediate takes part in a stable six-membered ring that favourably delivers the desired product. After the Heck reaction, a simple ester reduction was necessary to provide the target molecule in 68% yield.
Still exploring the Heck-Matsuda arylation of allylamine derivatives, Correia and co-workers recently reported new and concise synthetic routes for the lab-scale preparation of three other drugs: cinacalcet hydrochloride, alverine and tolpropamine.7676 Prediger, P.; Da Silva, A. R.; Correia, C. R. D.; Tetrahedron2014, 70 , 3333. Cinacalcet hydrochloride (167) is a calcimimetic drug commercialized under the trade names Sensipar and Mimpara, which is therapeutically useful for the treatment of secondary hyperthyroidism and also indicated against hypercalcemia in patients with parathyroid carcinoma. In the new synthetic approach, the main carbon skeleton of the final target was assembled through the arylation of allylformamide 168 with diazonium salt 169 leading to the adduct 170, which was in situ reduced, taking advantage of the palladium content from the arylation step, to afford intermediate 171 in excellent yield for the two steps. It is worth noting that the reduced steric volume of the nitrogen formyl protecting group proved to be essential for the observed high reactivity and regioselectivity of the arylation step. The deformylation of 171 under acidic medium with concomitant hydrochloride formation completed the synthesis of 167 (Scheme 44).
Alverine (172), a smooth muscle relaxant used for the treatment of gastrointestinal disorders such as diverticulitis and irritable bowel syndrome, was prepared from N,N-diallylacetamide 173 by means of a double Heck-Matsuda reaction/hydrogenation: a one-pot sequence furnished 175, in 72% yield, after reduction of the Heck adduct 174 in a tandem fashion. Acetyl group reduction of 175 with AlH3 afforded the final target in excellent yield (Scheme 45).
For the preparation of tolpropamine (176), an antihistaminic drug used for the treatment of allergies, a synthetic strategy involving a step-by-step bis-arylation of the allylamine derivative 177 was envisioned for the construction of the non-symmetrical diarylmethane fragment present in the final target. However, an initial model study, aiming at the preparation of the simpler symmetrical bis-arylated product, revealed some difficulties: although the terminal mono-arylated adduct 178a could be isolated in the pure form after the first arylation step, the second arylation reaction showed only moderate regioselectivity, leading to a mixture of the isomeric products 179a and 179b, which displayed very difficult separation (Scheme 46).
Thus, a new synthetic strategy featuring the Heck-Matsuda arylation of a methyl cinnamate derivative was proposed. Unfortunately, the appropriate combinations of cinnamate substrates and aryldiazonium salts, which could lead directly to the coupling product with the substitution pattern present in the final target, were found to be unreactive under the studied conditions.
Alternatively, the Heck-Matsuda reaction involving the corresponding halogenated coupling partners 180 and 181 led to the diaryl product 182 in excellent yield. In situ double bond hydrogenation and concomitant dehalogenation afforded the properly substituted intermediate 183 in 92% yield for the 2 steps. Ester hydrolysis followed by amidation and reduction reactions completed the synthesis of 176 (Scheme 47).
8. Conclusions and Perspectives
The ongoing efforts towards the use of Pd-catalyzed C-C cross-coupling reactions for the synthesis of drug components or drug candidates highlighted in this review demonstrated how powerful these methodologies are. The Suzuki reaction is the most exploited cross-coupling reactions, especially when the creation of a biaryl motif is involved. One of the main advantages of this methodology is the use of air- and water-stable, non-toxic and ease to manipulate organoboronic acid and its derivatives. Heck reactions are also intensely exploited for inter or intramolecular coupling involving Csp22 Beletskaya, I. P.; Cheprakov, A. V.; Chem. Rev.2000, 100 , 3009. double bonds. Despite the success of the coupling reactions, several challenges need to be overcome in order to have an economic, robust and reliable industrial process. Scale-up from the small (medicinal chemistry synthesis) to large scale usually requires new Pd catalyst precursors and several modifications to the reaction parameters. From the economic standpoint, in some cases, the cost of the precious metal palladium, as well as the ligand itself, can be a problem. There is a plethora of palladium catalyst precursors described in the literature that promote the C-C cross-coupling reactions. However, when these reactions need to be applied for the synthesis of complex molecules such as drug components or drug candidates, a few simple catalyst precursors are usually the first choice: Pd(OAc)2, Pd2(dba)3, Pd/C, PdCl2L2, Pd(PPh3)4. For aryl iodides or some very activated aryl bromides, ligand-free Pd catalysts can be used. However, in most of the reactions involving the synthesis of drugs, a phosphine ligand is necessary and triphenylphosphine is the most frequently used ligand. This cheap and stable phosphine is usually used already complexed in the Pd precursor (PdCl2(PPh3)2 and Pd(PPh3)4) or in association with a simple Pd precursor (Pd(OAc)2 or Pd2(dba)3). When a bidentated ligand is necessary, dppf or other ferrocenil-based bidentated phosphine ligands are used, with PdCl2(dppf)2 being the catalyst precursor of choice. Economically more attractive aryl chlorides have been used in some cases due to the development of electron-rich- and steric demanding phosphine ligands. However, the quest for precursor catalyst and ligands that allow high TON and turnover frequency (TOF), that would make the process economically attractive is still a challenge. Another important point that needs to be taken in account is the contamination of the product with palladium and the need for further purification steps. Therefore, practical and feasible catalytic systems that allow the easy recovery of palladium or coupling reactions involving cheaper transition metals are welcomed.
This article is dedicated to the memory of Prof Roberto F. de Souza
We thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS), PRONEX-FAPERGS and the Instituto Nacional de Ciência e Tecnologia de Catálise (INCT-Catálise) for partial financial support. We also thank the CAPES (J.L., A.F.P.B, and T.S.C.) and PETROBRAS (C.S.S.) for scholarships.
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Publication in this collection
28 Aug 2014
04 Nov 2014

domain: chemistry


text:
This grain bowl recipe checks all the boxes: it has plant-based protein, lots of veggies, a vibrant sauce, and a scoop of sauerkraut for pop!
This grain bowl recipe is Day 1 of a meal planning week here on Love and Lemons! Why? Well first and foremost, it’s because our meal planning journal, The Love & Lemons Meal Record and Market List, is out today! If you missed my post last week, click here to read all about it. If you haven’t pre-ordered one, click here to get one now!
When I write about meal prep or making quick, easy dinners throughout the week, I often suggest cooking components on Sundays in big batches. I like this approach, but this week I’m doing something a little bit different. Starting with one meal – this yummy, easy Roasted Veggie Grain Bowl – I’m going to transform the same components into a new dinner every night for 5 nights so that each new meal builds off of the previous one. Find the recap for the whole week here!
How to Make an Awesome Grain Bowl
A grain bowl was a natural choice for the start of our meal plan week, because all good grain bowl recipes rely on components. I used them first in this grain bowl recipe and then mixed and matched them into other meals throughout the week. Here are the component categories I started with:
- A grain: I use quinoa! It’s naturally gluten-free, and it packs a punch of plant-based protein.
- Plant-based protein: I use chickpeas, one of my go-tos. Feel free to roast them for extra crunch!
- A yummy sauce: A super-bright, vibrant kale pepita pesto.
- Fresh vegetables: I top this bowl with roasted cauliflower, broccoli, Brussels sprouts, and parsnips for a surprising sweet twist.
- A pickle: Sauerkraut adds a tangy, briny twist to this healthy quinoa bowl.
- Something extra: Top it all with pepitas for crunch!
Load these components into this grain bowl recipe tonight. Then, mix and match them into the other recipes in the meal plan throughout the week!
I love making this grain bowl recipe as part of my 5-day meal plan, but it’s also a great template for making a DIY grain bowl at home. To make your own bowl, think in terms of the categories I listed above:
- A grain: Use quinoa, or try farro, white or brown rice, cauliflower rice, or even wheat berries or millet!
- Protein: Think baked tofu, tempeh, black beans, or lentils. If you’re not vegan, try topping your bowl with a poached or fried egg!
- A yummy sauce: Peanut sauce, chipotle sauce, cilantro lime dressing, basil pesto, or tahini sauce would all be delicious.
- Fresh vegetables: Any roasted or raw veggie is at home in a grain bowl! Mix and match veggies according to the flavor profile you’re going for. Experiment with grilled corn, sweet potato, bell pepper, cabbage, cherry tomatoes, and more.
- A pickle: Pickled onions and jalapeños are two of my favorites.
- Something extra: I like to add nuts and seeds for crunch, but feta cheese and fresh herbs are yummy add-ons too.
Let me know what variations you try! In the meantime, you can record your own meal plans here in our pretty (and pretty useful) new meal planning journal .
More Favorite Grain Bowl Recipes
If you love this grain bowl recipe, try making one of these tasty bowls next:
- Sweet Potato Quinoa Bowl
- Mango Ginger Rice Bowl
- Sesame Soba Noodles
- Best Buddha Bowl
- Portobello Burrito Bowl
Roasted Veggie Grain Bowl
- 1 cup raw quinoa, rinsed
- 1¾ cups water
- ½ cup pepitas (or shelled raw pistachios)
- 2 small garlic cloves
- 1 packed cup chopped kale
- 1 packed cup cilantro, more for garnish
- ¼ cup fresh lemon juice
- ½ teaspoon sea salt
- freshly ground black pepper
- ½ cup extra-virgin olive oil
- ½ cup water
- ½ teaspoon maple syrup or honey
- 2 parsnips, chopped into ½ inch pieces
- florets from ½ cauliflower
- ½ bunch broccolini
- 1½ cups halved Brussels sprouts
- 1 (14-ounce) can chickpeas, drained & rinse, use ¼ cup per bowl, save the extra
- scoop of sauerkraut (I like Bubbies)
- sprinkle of toasted pepitas
- Preheat the oven to 425°F and line 2 baking sheets with parchment paper.
- First, make the quinoa. Add the rinsed quinoa and water to a medium pot. Bring it to a boil, cover, reduce the heat, and simmer for 15 minutes. Remove from the heat and let it sit, covered, for 10 more minutes. Fluff with a fork. This will yield about 3 cups; I used a heaping ½ cup per bowl.
- Next, make the sauce. Combine the pepitas, garlic, kale, cilantro lemon juice, sea salt, pepper, olive oil, water, and maple syrup or honey in a blender and blend until smooth.
- Then, roast the vegetables. Place the parsnips, Brussels sprouts, and cauliflower on one large baking sheet. Place the broccolini on the second baking sheet. Drizzle the vegetables with olive oil and pinches of salt and pepper, toss to coat, then spread evenly onto the sheets. Roast the parsnips/Brussels sprouts/cauliflower 20 to 25 minutes or until golden brown around the edges. Roast the broccolini for 10 to 12 minute or until tender. When cool to the touch, chop up the broccolini stems.
- Assemble bowls with a scoop of quinoa, the roasted vegetables, about ¼ cup chickpeas, and a scoop of sauerkraut and top with pepitas. Drizzle with the sauce. Season to taste with additional salt and pepper, if desired, and serve. I assembled these components into 2 bowls (although the whole recipe will make 4) and saved the leftovers for tomorrow’s dinner - stay tuned!
- Store the extra sauce, quinoa, and remaining chickpeas in the fridge.
great recipe! Thank you for this!
So glad you enjoyed it!
Love this make ahead lunch! Easy variations to try as well. Have been using Trader Joe’s Green Goddess and Cilantro dressings. Sauerkraut is awesome!
I’m so glad you’ve enjoyed it!
I’d love to make this pesto, but I have a nut allergy. I’m curious what I can substitute the pistachios with!?
Hi Sophia, I love to make it with pepitas, they give it a nice vibrant green color too.
I didn’t have kale so I used spinach….delicious with roasted veggies (turnip, sweet potato, beet, carrot,onion, butternut squash, and zucchini). I used farro. Thanks
Hi, did you measure veggies i 4 bowls or 2? I have that same mix and was curious how much roasted veggies per svg?
Love, love, love this recipe!! While the pesto seems lacking when you taste test it on its own, it’s perfect once you mix everything around. We use cannellini beans instead of chickpeas, only because we like Them better; both are good. And this DEFINITELY makes excellent leftovers! This has entered our regular rotation of meals!
How much calories do these bowls have
This is probably a daft question but what size of bowl would you typically use for these? (We only have cereal/soup bowls which aren’t really suitable for these). I’m looking forward to creating some exciting lunches that aren’t sandwiches!
Hi Claire, a big cereal bowl could work – I have some bowls that are a bit larger (usually sold as “pasta bowls”) that I also like to eat large salad lunches out of.
Thank you! 🙂 I’ve found something suitable now.
Can you post the macros?
This pesto is a delicious way to brighten up the roasted veg and grains (I used faro). It’s very easy to make.
Solid meal tonight! Looking forward to tasting the remaining installments!
so glad you enjoyed the bowls! 🙂
This is amazing! So easy and delicious. Thank you so much for this great meal plan – it is so good to learn how to put things like this together and very budget friendly (even in NZ where groceries can be killer!) Very grateful – thanks again!!!
The Creamy Kale Pepita Pesto looks amazing!!!
As an salad addict, I always try something new everyday.
Yours looks delicious with the roasted vegetable as my favourite ingredients <3
I'll try it soonn!!
I’m so excited about making this, I want to toss my to-do list out the window and head to the kitchen right now!
ABSOLUTELY delicious! Grabbed the ingredients and made this for dinner tonight. Excellent flavor. The only changes I made were to use red sauerkraut and broccoli (vs broccoli). Nervous how hubby would feel about a meatless meal I dry-poached some organic chicken breasts and added that. Two thumbs up!!!
I’m so glad you liked it! Happy you found the recipe to be so flexible too 🙂
ha ha 🙂

domain: food


When you think of Kerala, you conjure up images of coconut groves swaying in the breeze, houseboats drifting across backwaters, vast paddy fields enshrouding verdant villages, and vibrant cultures thriving in colours of the land.
To capture this essence of a state blessed by nature, needs a special kind of place. And Coconut Lagoon in Kumarakom has managed to do just that. Shrouded in the lyrical legacy of the destination, it takes you to Kerala of the magical past.
In the 25 years of its existence, Coconut Lagoon has become one with the landscape of the region, assimilating its soul and exuding its spirit. The iconic property is now a quintessential part of travellers’ itineraries who seek to experience Kerala at its intimate best.
Coconut Lagoon can only be accessed by water. Drift through the unflustered Vembanad Lake to be welcomed by a gorgeous play of colours, where the skies and the waters mingle on a canvas laid out by nature itself. Serenaded by tranquil streams flanked by lush coconut groves, each moment spent here takes you on a mesmerising journey of Kerala’s wonders.
The living spaces here are the centuries-old ‘tharavad’ mansions – rescued from demolishment, painstakingly dismantled, and refurbished piece by piece in Coconut Lagoon. There are three types of accommodation – the Heritage Bungalow, an independent cottage by the canals, the spacious two storied Heritage Mansion and the Lake Front Villa with Private Pool.
Life in Kuttanad follows the languid pace of a ketuvallam floating across Lake Vembanad. The community here derive sustenance from the lake through fishing, agriculture, boat building, handicrafts and tourism. As you traverse along the banks of the waters, lined with traditional cottages, you will get a glimpse of the village folks’ lifestyle. Witness fishermen and shell harvesters at work, farmers harvesting paddy, and toddy-tappers clambering up the coconut trees. Each experience bringing you closer to the authentic backwater life.
The dishes at Coconut Lagoon are prepared echoing the scents, ingredients and intricacies of an authentic Kerala kitchen. Discover the poetry in cinnamon bark, mingling with jaggery; crayfish roasting in pumpkin leaves; or the fresh catch of the day, grilled with the choicest native spices. Not to be missed is the paddy hut dining experience that immerses you in the romance of Kerala.
At Coconut Lagoon you will unearth the joys and secrets of Kerala’s culture. Be it the ‘Chaya Chechi’ delivering tea and snacks on her floating tea shop. Or the dazzling performances of Kathakali, Mohiniyattam, Ottanthullal and more. Dive into the bio-diversity of the Kuttanand region with a village expedition and a bird-watching tour with a naturalist. Or indulge in a sunset cruise across the backwaters.
CGH Earth has always worked with the local community, supported the local ethos and carried out environment-friendly practices. There are no walls between Coconut Lagoon and the surrounding village, physical or otherwise. The people who take care of you during your stay at the resort, hail from the local community, and are brimming with home-grown wisdom and stories. Coconut Lagoon is dedicated to preserving not just the culture, but also the fragile ecosystem. To minimise waste, all the wet waste is converted to biogas for cooking fuel, and vermin-compost for manure. The few paper and plastic items that are discarded as dry waste are up-cycled into building materials or decorative pieces. CGH Earth’s commitment to protecting the ecosystem extends to the local fauna. Coconut Lagoon is home to a rare breed of cows from the neighbouring Village, Vechoor. This indigenous cattle breed is dying out, but at the resort you will find them grazing peacefully on the lawns. Coconut Lagoon is more than just an extension of the village; it is a celebration of the region.
As much as we indulge in the joys of nature, so are we destined to face its fury. The recent floods in Kerala devastated lives and livelihoods, but failed to shatter the spirit of the people. Today, in the aftermath of the disaster, as the state is getting back on its feet, it has displayed an indomitable resilience. Coconut Lagoon has imbibed the same spirit, and after bearing the brunt of the floods, is now back in business. It’s a resurgence that’s testament of the character of the land, one that is ready to rough out any weather to emerge stronger.


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Go behind the scenes with the star of Leonardo, Jonathan Bailey, as he shoots a dramatic flying scene.
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We work with a wide range of other organisations including housing associations, Devon Rural Housing Partnership, Safer Communities, Racial Equality Council and Local Enterprise Partnerships to address housing issues across all tenures.
We also build and purchase homes to sell affordably on a shared ownership basis, or using the Help to Buy scheme. South West Homes is the Help to Buy agency for all seven counties in the south west, managed jointly by Westcountry Housing and Sovereign Housing and provides shared ownership and other equity products to help people buy their own home.
Westward initiative, Horizon Homes, sells properties on the open market to both augment neighbourhoods and to provide financial support for the continuing development of our core business, meeting our social purpose.
The affordable housing sector in the south west plays a key role in the local economy creating 11,000 jobs; valued at a £1 billion turnover.


text:
El Nino de 272. “Welcome to Wyckoff Gardens” documents a community facing the lingering uncertainty of a newly gentrified Brooklyn. Located in the Boerum Hill neighborhood, Wyckoff Gardens consists of three twenty-one-story buildings with 528 apartments housing approximately 1,226 people. Dating back to its earliest years Boerum Hill had served as a melting pot of cultures, ethnicities, and classes, but with rise of brownstones, hotels, condominiums, and commercial businesses, the area is rapidly becoming one of the most expensive and highly sought after neighborhoods in all of New York City.
Coming of age in Wycoff Gardens, my early memories of this area is one of a neighborhood untouched by these changes. Upon first glance, the project complexes felt practically segregated in this neighborhood, worlds away from the newly erected brownstones and condos. Wyckoff Gardens now felt more like relic of a bygone era, with its inhabitants serving as guardians. However, recently the area has been visibly impacted by the phenomenon of gentrification, with the cost of living increasing and commercial structures being erected close by. Historically, the media has portrayed Wyckoff Gardens as a dangerous and impoverished part of Brooklyn, a proverbial no mans land; this series aims to shed light on the true nature of this community, effectively voiding these stigmas. "Welcome to Wyckoff Gardens" celebrates this diverse working class as seen through the eyes of its inhabitants.

domain: sociology


In the midst of the Clipper, I also cast on Daughter's thrummed mittens but the real adventure had begun a few days before.
I had lots of white fiber for the thrums but she wanted mittens in her skating team colors which are black, white and red.
I had black yarn so I needed to come up with some red fiber. I used my dyeing crock pot and hoped for the best. I've never had any luck with it and red is hard in the best of circumstances.
Well, look at that. That white knife is in the rinse water with the newly dyed and fiber and nothing is bleeding. That's a first.
After a spin in the salad spinner, I had some pretty red Corriedale that needed to dry for a few days before I could turn it into a pile of thrums.
I'm using the red and white on every other row and I'm trying to do two thrum rows a day. I might not have these done for her by this winter but next year's a lock.
It's so nice when a plan works out! Very classy!ReplyDelete
You could not have made more perfect red. Looking great!ReplyDelete
So pretty! The red and white really pops with the black yarn.ReplyDelete
Cool! Thrums are on my list to try also. I find black yarn reallly tough to knit.ReplyDelete


Revealed: The behaviour commuters hate the most on public transport (if you want to make friends, best to put your phone away)
- Your commuting habits could make you the bug bear of fellow travellers
- Those who take up too much room are among the most hated for Brits
- But eating, having bad body odour and making calls are also annoying
Do you have a habit of eating on the train or constantly making calls on public transport?
If so, you could be unwittingly making your fellow travellers hate you.
These two traits were picked out as two of the worst in a new survey of British commuters, who were asked what behaviour in others annoyed them the most as they journeyed to work.
Commuters hate people who take up too much space, according to the results of a new survey (file photo)
But the survey, conducted by Joosr, revealed that the thing that annoys the most number of commuters - 38 per cent of them - are fellow passengers who take up too much space.
For 36 per cent of respondents, people who make phone calls are extremely annoying - even though more than a quarter of those surveyed were also making the calls themselves.
Another 34 per cent of people find bad body odour vexing, while it's passengers who eat on public transport that bugs 24 per cent of people.
Interacting with fellow commuters is frowned upon as well.
Of those surveyed, 17 per cent said they hated making eye contact while 16 per cent did not enjoy having to talk to other commuters.
But it's not that commuters don't enjoy communicating because 29 per cent of people get annoyed about the lack of phone signal, which means they can't surf the web or send and receive calls and texts.
Don't eat while you're on public transport as it's extremely annoying for 24 per cent of commuters (file photo)
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On Monday, October 24th, 2022, Jefferson County Sheriff's Deputies were dispatched to China Elementary in reference to a burglary that occurred over the weekend. School faculty noticed open doors, missing items, and some minor damage to parts of the building.
Upon reviewing security camera footage, two white males on bicycles were seen circling the building on Sunday evening. They then broke into the building a short time later, going through several classrooms and the cafeteria, and stealing audio equipment. With assistance from school faculty, Deputies were able to locate one of the stolen pieces of equipment near a wooded area several miles away.
On Wednesday morning, October 26, 2022, Deputies located the two bicycles seen in the security camera footage and made contact with two white males, 17 year old Christopher James Standifer of China and 17 year old Lukas Allen Waller of Liberty.
After meeting with Detectives, both confessed to committing the burglary. Arrest warrants were issued by Judge Ben Collins and both suspects were transported to the Jefferson County Correctional Facility.
Detectives are still searching for one large PA-style speaker which may have been abandoned in the area of Henderson Avenue in China. Please contact the Jefferson County Sheriff’s Office with any information regarding the whereabouts of this speaker. 409-835-8411.
10/27/2022 12:41 PM
Crystal Holmes, Captain


Moroso 73684 - Moroso Ultra 40 Unsleeved Spark Plug Wire Sets
Product images may differ from actual product appearance.
JEGS Part Number: 710-73684Ultra 40 Unsleeved Spark Plug Wire Set
Moroso Ultra 40 Unsleeved Spark Plug Wire Sets
Ultra 40 Unsleeved Spark Plug Wires are a cost effective version of the Ultra 40 Race Spark Plug Wire without heat sleeving. Wires feature a Kevlar core, four times stronger than other aramid fiber cores, that increases the tensile strength of the wire while reducing its center diameter. This allows for the surrounding insulation to be thicker, resulting in greater dielectric strength. With only 40-ohms of resistance per foot these wires will increase power and fuel efficiency by bring more spark to the plug. RFI/EMI resistant to eliminate stereo feedback or ignition interference.Features:
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