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NASCAR driver, North Carolina native and Hellmann’s partner Dale Earnhardt Jr. joined Hellmann‘s in Toast to help encourage the removal of mayonnaise among locals.
Dale also advocated for locals to try ‘mayo on the outside‘ grilled cheese sandwiches for free at Hellmann‘s Pop-Up, located on the outskirts of town at the Mount Airy Food Lion. Some skeptics were converted into mayo grilled cheese believers!
Hellmann’s billboards and signage along the roads leading into town warned locals of the mayonnaise removal beginning March 14.
Dale also went door-to-door removing mayo from homes and asked local business and restaurants to join in on the prohibition of mayo. Even the mayor joined the brand in the Hellmann‘s removal.
Hellmann‘s shared their own Perfectly Grilled Cheese Recipe for folks to continue enjoying this new way to make toasty sandwiches leading into National Grilled Cheese Day on April 12.
— Edelman —

domain: news


text:
East Alton pondering changes to trash pickup
EAST ALTON — Officials are hoping small changes to the trash pickup schedule will generate noticeable cost savings and added efficiency for the village.
The village is anticipating in the coming weeks rolling out a new trash pickup schedule that will reduce the number of garbage trucks needed from two to one, East Alton Mayor Joe Silkwood said, generating around $35,000 in savings, mostly by way of part-time employee hours worked.
The new schedule, which is still being tweaked, would also hopefully free up some time for full-time employees to focus on other tasks, Silkwood said.
“We’re just going to try to run it as efficiently as we can, and still offer the same great service that we offer,” Silkwood said.
The changes likely wouldn’t affect everyone, Silkwood said; in fact, most village residents would keep their same pickup day. Village employees on Monday were running test routes to get a better sense of what might be workable, but no changes to routes or pickup days have yet been enacted.
The biggest change for residents may be regarding recycling pickup. Currently, all recycling is picked up on Thursdays. Silkwood said a change to the recycling pickup day is among the things still being discussed.
When changes are finalized, Silkwood said the village will ensure everyone is notified by way of flyers, local media stories and Facebook postings. The changes won’t require official action by the village board.
Reach managing editor Nathan Grimm at 618-208-6456.


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Reporting from Nazi Germany 1938-40.
'This Is Berlin' gathers together the previously unpublished extraordinary broadcasts and writings from Berlin of William Shirer, the acclaimed American journalist whose 'The Rise And Fall Of The Third Reich' quickly became, and still remains, the standard work on Nazi Germany. Here is a vivid, compelling and urgent narrative, one of the great first-hand documents of the Second World War.
All through the campaign - from the Anschluss to the fall of France, Dunkirk and the Battle of Britain - Shirer proved himself a master chronicler as he offered the world a unique and dramatic by-line on history as it happened inside Hitler's Germany.
- Publication Date:
- 06 / 04 / 2001
- 128 x 196mm

domain: library


This content has been marked as final. Show 3 replies
you can easily create insert statements for your tables which can populate as many records as you want. Also creating insert statements to populate data for different conditions will help you in logic formation, below e.g. this is trivial but you can go ahead and form your own statements.
Also "crores" would not be easily followed by many so use terms like million or billion or 100k or so depending on what you want to represent.
insert into test(col1,col2,col3,col4,col5,col6) select rownum rn,rpad('x,100,'x'),rpad('x,100,'x'),rpad('x,100,'x'),rpad('x,100,'x'),rpad('x,100,'x') from dual connect by level <= 1000000;
Random data will only get you so far. It's fine for some types of bulk testing but it has two major flaws:
1) A lot of performance issues derive from skew in our data. Randomly generatedly values , while exhibiting clumps of values is unlikley to have the extremes of data distribution which we see in real data. This includes things like variation in string length.
2) Generating keys are difficult. Sure, we can generate unique numeric keys with ROWNUM but other types or uniqueness are harder and wrangling foreign key relationships is a complete haemorrhoid.
So, what to do? Well there are a number of data sets out. The best place to look is [url http://www.infochimps.com/datasets]InfoChimps. This used to be a really great site but the company is (not unreasonably) seeking to make money from theit efforts, so they restrict now access to a lot of their data sets. Nevertheless many sets are free (although reigistration is required) or else just links to externally hosted public data sets.
Most of the data sets are CSVs, so there is a certain amount of work required to get them into a database. However, it's not too difficult with external tables, and that is also a useful training in its own right.
You could do what Oracle support does:
1) Get the schema (or your own schema)
2) Load the statistics from the origin (or you could set them as high volume as you need).
3) Then, you should get an explain plan if not exact, but close to the reality expected.


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Secretary of Defense Leon Panetta is scheduled to visit Vietnam next week, as part of his trip to three Asian countries. Secretary of Defense Leon Panetta . The website of the US Department of Defense cited the Pentagon spokesperson George Little as saying that Mr. Panetta to visit Vietnam and India following the International Institute for Strategic Studies Shangri-La Dialogue in Singapore on May 30. On the way to Asia, he will visit with head of the U.S. Pacific Command, Navy Adm. Samuel J. Locklear III, at his headquarters in Hawaii, as well as with service members assigned there. The secretary then flies to Singapore to deliver remarks at the opening plenary session of the Shangri-La Dialogue where he is expected to speak about U.S. defense policy in Asia-Pacific. He will also hold bilateral meetings with his counterparts from Singapore, Japan, the Republic of Korea, Australia and other nations. Panetta will then travel to Vietnam for a two-day visit. “The United … [Read more...] about US Secretary of Defense to visit Vietnam
When to visit vietnam
A Visa survey on world travel in 2011 indicates 24% of international tourists who plan to visit Vietnam in the coming time come from Singapore and Thailand. Beautiful natural landscapes, appealing package tours and the stable political environment are the three key factors making Vietnam an ideal destination for foreign tourists over the next two years, according to the survey. Overseas travelers are keen on outdoors activities and gourmet tours so that they can get a feel of bustling nighttime life and delicious specialties in Vietnam. The survey found 64% of tourists are willing to pay a premium price for culinary delights, and 63% want to discover major tourist destinations and take cultural tours. Around 40% of tourists are interested in traveling on their own in Vietnam, said Lorijon Bacchi, director of Visa in Vietnam, Laos and Cambodia. Most of the travelers who visit Vietnam have already toured Asia's tourism destinations namely Hong Kong, China and Malaysia. Those … [Read more...] about More Asian tourists to visit Vietnam
Jan Cempirek has become famous in the Czech Republic after he was revealed as the author of White Horse, Yellow Dragon, which had won the annual award of a prestigious book club in Czech under the name of a Vietnamese girl: Pham Thi Lan.Cempirek observed: “I have to continue this game because it opens a new topic, with new factors . . . and I plan to write about this ploy.” Does this game end? And how does it end? Saigon newspaper interviewed Jan Cempirek to seen the answers. Tiep Thi Saigon: Congratulation, Mr. Cempirek! You are now not only famous for winning a significant literary award, but also a talented actor who played another character. When did you have the idea to create Pham Thi Lan and why? Tiep Thi Jan Cempirek: The first time I thought of an alter ego for my novel was ten years ago. At that time, my friends and I performed an amateur play based on Boris Vian’s L’Herbe Rouge book. This French writer wrote novels under an alter ego – … [Read more...] about Scandal-ridden Czech writer to visit Vietnam
President Sang hails new era of Viet Nam - Singapore partnership President Truong Tan Sang yesterday (Sept 12) praised the establishment of a strategic partnership between Viet Nam and Singapore which marks the 40th anniversary of diplomatic ties between the two countries. Meeting with Singaporean Prime Minister Lee Hsien Loong in Ha Noi yesterday, the President expressed his optimism for the new partnership, which marks a major development in bilateral relations. He said he believed co-operation would strengthen in investment, trade, education and cultural pursuits; adding that Singapore's stance on the East Sea issue and the countries' shared experiences in the region had created trust between the two countries. He urged the two sides to develop detailed plans to implement signed agreements efficiently. Prime Minister Lee Hsien Loong agreed, saying that he would instruct ministries to build co-operation programmes to implement their bilateral obligations. He said Viet Nam … [Read more...] about New Zealand Deputy PM to visit Vietnam
Phu Quoc Island is one of the most popular resort destinations in southern Vietnam. Photo: Shutterstock Planning your holidays in Vietnam, you might wonder when it is the best time to visit the country. The answer is quite simple: anytime is the best time! No, but seriously. I think it depends on how you would like to spend your holidays in Vietnam. If you want to stay at a nice and cozy beach resort, sipping cocktails and getting a tan, you will want to set your timing different than if you want an active holiday. But first things first: Vietnam is a long country, stretching over a thousand miles, from Sa Pa in the north to the Mekong Delta in the south. There are roughly two different climates, the northern climate with four seasons and the tropical climate with only two: dry and wet. The meteorological divide between the two main climates is the Hai Van Pass between Hue and Da Nang. Climate of the south Let's start in the south of Vietnam. The dry season lasts from December to … [Read more...] about When is the best time to visit Vietnam?

domain: travel


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Students Devise Products For Adults With Dementia
Anyone who has, or had, a loved one with dementia will want to say “thank you” to the seven student finalists of the inaugural Stanford Center on Longevity Design Challenge announced today. With any luck, caregivers and families will be able to buy their brilliant creations sometime soon.
Standford, in collaboration with Aging 2.0 (an organization aiming to accelerate innovation to improve the lives of older adults), challenged graduate school and undergraduate students around the world to design products and services that would keep individuals with cognitive impairment independent for as long as possible.
Winner: Ani Agarian: "Motorics/An Age of Confidence"
An engaging gaming experience teaches the elderly the actions with touchscreen devices that can help them to use any kind of applications available in the market today. The application will support the users' independence and wellbeing.
Posted on healthcaredesignmagazine.com on November 5, 2013, by Anne DiNardo, Senior Editor
The importance of artwork in the healthcare environment and its role in the healing process has become more widely accepted over the years. But that still doesn’t mean it’s always part of early design discussions.
Rather, art programs are often addressed later on in the project schedule, maybe even after the drywall is up, leaving art consultants and interior designers to scramble to find appropriate pieces that fit on existing walls and ceilings.
It’s a practice that Janelle Baglien, president, Studio Art Direct (Portland, Ore.), calls “plunk art.” “It’s when the building’s all done and somebody says ‘oh, we have to plunk something here.’”
When art programming, themes, and budgets can be established early on, the benefits can be plentiful, including site-specific installations, color palettes, and works that support the overall interior design package.
At Kaiser Permanente’s new Westside Medical Center, in Hillsboro, Ore., the art program was anything but an afterthought as the owner set out to “make the best patient environment through the use of such elements as daylighting, natural views, and outdoor awareness,” says Willy Paul, executive director, national facilities services northwest, Kaiser Permanente. “Art also plays a big part.”
Kaiser established an overarching theme, “tranquil relief through nature,” to help guide the design of its 38th hospital, which includes a 126-bed hospital and 110,000-square foot medical office building (MOB). Then it hired Baglien early on to procure an art program with AECOM (Minneapolis), which handled the architecture and interior design. Themes were created for each floor, such as forests, water, wildflowers, and mountains/long-distance views, with corresponding wall colors, flooring, carpeting, and artwork.

domain: artistic


If you’re in the market for a pair of Bluetooth earbuds, few options are as attractive as the Apple AirPods. They’re sleek, low profile, and offer outstanding performance, but at $200, few are willing to buy a pair. Luckily, if you’re simply interested in Apple’s aesthetic, you can get these Apple-inspired Bluetooth earbuds for just $24.99.
The AirSounds 2 True Wireless Bluetooth Earbuds give the AirPods a run for their money. They feature Bluetooth 5.0, providing crisp audio and an operating distance of up to 33 feet. You can enjoy up to 4 hours of playback off a single charge, while the included charging case offers an additionally 8 hours of battery life. The AirSound even come with auto pairing and touch controls, making setup and day-to-day usage as easy as ever.
With smartphone manufacturers getting rid of the headphone jack, we’ll all have to make the jump to Bluetooth sooner or later. With the AirSounds 2 True Wireless Bluetooth Earbuds, you can enjoy the advantages of Bluetooth now for just $24.99, or 63% off.
Take a load off with some good news as we head into the weekend
We're headed into the weekend, so why not kick it off with some good news? Today, we've got stories of trees planted, masks delivered, and esports taking over ESPN (for a little while, anyway).
Join us for the latest Windows Central Video Podcast
We're live with the Windows Central Video Podcast today at 2:30pm ET, make sure you're there!
Review: Kensington's SD5500T dock works with USB-C and Thunderbolt 3 PCs
The SD5500T docking station is compatible with USB-C and Thunderbolt 3, opening it up to use with more devices. It's similar to some other great docks out there, so how does it measure up?
Best Zoom Alternatives in 2020
Zoom continues to face new vulnerabilities, exploits, and privacy concerns, with even the FBI issuing a warning over recent hijacking attempts. We've wrapped up the best Zoom alternatives if you choose to switch.


Dishwasher Water Feed Tube W11244157
This water feed tube (part number W11244157) is for dishwashers. Water feed tube W11244157 carries water to the upper spray components. Wear work gloves to protect your hands during the repair.
|Associated Number (1)|


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What is Electrical Heat Tracing
Electrical Heat Tracing is an engineered system designed to maintain or protect process piping, equipment, vessels, and instruments at defined temperatures and within specified design criteria.
An electrical heat tracing system is much more than just the heating cable. A large majority of
commercial electrical heat-tracing systems are of the resistive type and take the form of cables that are placed on pipes.
When electric current is flowing through the resistive elements, heat is created in proportion to the square of the electric current and the resistance of the elements to the current flow.
Industrial applications of heat tracing may be found in the oil and gas production, storage facilities, petrochemical plants, refineries, pharmaceutical production, power generation, water and waste treatment plants, food processing, plus many others. Solutions using heat tracing can be provided for freeze protection, or for temperature maintenance duties.
Heat tracing for frost protection ensures that the minimum temperature is not exceeded at any one point in time. Application areas are often supply lines. If these supply lines freeze, it would not only result in interruption to production, but would also attract considerable damage to the entire pipeline system. A competent heat tracing system would ensure safety and reliability and allow operations to run smoothly.
Some other typical application can include:
Frost protection for roof tops in cold countries.
Heat tracing systems for temperature maintenance have a wide range of applications. It ranges from the preservation of the fluidity of a medium to support for efficient production.
Some typical applications can include:
Gasoline oil, liquid asphalt, chemicals, chocolate, and vegetable oils.
Some heating processes require at times a particularly high performance/temperature, such as when restarting a column or a reactor. Once the target temperature is reached, the power can be reduced to regular temperature maintenance level. This flexibility allows the heat tracing system to be versatile and only use more energy when necessary.
Benefits of Heat Tracing
Electrical Heat Tracing are the most efficient type of heating as compared to for example, steam tracing. The energy supply can be brought to any location with virtually no losses in energy to be converted into heat. You can find out more about electrical heat tracing vs steam tracing here.
If service is required, the area that is to be repaired is easily accessible and can be maintained with little effort after switching off the heating system.
Electrical heat tracing systems are easy to design, are rugged and easy to operate when installed by a competent expert specializing in the heat tracing industry.
We hope that you have a better understanding of the various applications for electrical heat tracing.
As an expert in the field of heat tracing with many years of experience and having completed various large-scale projects. Supermec provides both constant wattage and self-regulating trace heating systems. Get in touch with our friendly electrical heat tracing experts at email@example.com or you can drop us a call at +65 68619522 to find out how we can assist you with your heat tracing needs and install a heat tracing system to help your business objectives today.


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Steve's guests include Bee Gee Robin Gibb and TV presenter Patrick Kielty.
BBC Radio 2
Steve and the team bring you the big show with great music and celebrity chat.
Guests include Sean Lock, and Deborah Meadon and James Caan from BBC2's Dragon's Den.
Guests include TV and Radio 1 presenter Vernon Kay, and West End star Michael Ball.
With film director and food critic, Michael Winner, and singer Marianne Faithful.
Steve and the team welcome Sandra Bernhard and Dr Hilary Jones.
With the BBC's World Affairs Editor John Simpson and musician Jools Holland.
Steve's guests include singer songwriter Alison Moyet.
With new and classic music, celebrity chat and the Big Quiz.
Among the guests on the show are Radio 1 DJ Chris Moyles.
Featuring top celebrity chef Gordon Ramsay, and singer and broadcaster Mica Paris.
New and classic music, and celebrity chat with cricketing legend Sir Ian Botham.
Music and celebrity chat. Guests include Alan Titchmarsh and soap star William Roache.
Mark and Emma are joined by actors Martin Freeman and Colin Salmon. Plus Katie Melua.
Guests include actor Alan Alda and comedian Paul Whitehouse.
Mark stands in for Steve, and is joined by comic actor Peter Serafinowicz.
Mark sits in for Steve Wright, and is joined by comic Jennifer Saunders.
Mark sit in for Steve Wright and is joined by Jason Donovan and Natascha McElhone.


text:
Stairway Floral Plein Air
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domain: design


text:
Maxine hong kingston (tongue tied) richard rodriguez (aria) gloria on this occasion, anzaldua's mother also tells him off as she was. Maxine hong kingston (tongue tied) richard rodriguez (aria) gloria anzaldua (how to tame a wild tongue) in the short story's 'tongue tied', spicy tongues: mother tongue by amy tan essay always loved language.
A first language, native language, or mother/father/parent tongue is a language that a person has been exposed to from birth or within the critical period in some .
In “aria”, richard rodriguez describes the social and cultural difficulties rodriguez's experiences are mirrored in amy tan's “mother tongue” in which tan.
As a child of emigrants from china, author amy tan knows from experience the problems that face children with a family that uses english as a second language . In amy tan's, “mother tongue” and richard rodriguez “aria: a memoir of a bilingual childhood”, both authors experience the difficulties of language barrier and.


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For more information on KPFA's archiving policy go here: Learn More.
An hour-long news magazine with a strong focus on state and local issues, featuring hard-hitting interviews, debates, and in-depth reporting from journalists working everywhere from Los Angeles to Oakland to Sacramento.
Up Front is produced by KPFA's award-winning News Department. Listeners can look forward to challenging interviews with political and community leaders, civil but heated debates, and frequent breaking news updates.
About the host:
Brian Edwards-Tiekert is a radio journalist who has won multiple awards for his feature reporting and radio documentary work on environmental issues. He's the former co-host of The Morning Show, at the time KPFA's top-rated local program.
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Fukushima, Student Debt, Pensions Debate
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We continue our series on boomtimes and housing costs in San Francisco with a history of the social movements that have formed to fight development and displacement. Karl Beitel joins us in studio to discuss his new book *Local Protest, Global Movements: Capital, Community, and State in...
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PM Modi, Tendulkar congratulate Hima Das for dream run in Europe
"India is very proud of @HimaDas8’s phenomenal achievements over the last few days. Everyone is absolutely delighted that she has brought home five medals in various tournaments. Congratulations to her and best wishes for her future endeavours," PM Modi tweeted.
India is very proud of @HimaDas8’s phenomenal achievements over the last few days. Everyone is absolutely delighted… https://t.co/EPd5qLbHMO— Narendra Modi (@narendramodi) 1563717907000
Hima's latest gold came on Saturday in Prague where she clocked a season-best time of 52.09 seconds to win the gold in the 400m at Nove Mesto nad Metuji Grand Prix. Her personal best is 50.79 seconds, which she recorded at the 2018 Asian Games.
"Loving the way you have been running in the European circuit over the last 19 days. Your hunger to win and perseverance is an inspiration for the youth. Congrats on your 5 Medals! All the best for the future races, @HimaDas8. (sic.)" Tendulkar wrote on the social media platform.
Loving the way you have been running in the European circuit over the last 19 days. Your hunger to win and persever… https://t.co/zz1CdsMYk4— Sachin Tendulkar (@sachin_rt) 1563711104000
The Assam sprinter, however, failed to qualify for the World Athletics Championships as the 400m qualification mark for the world event has been kept as 51.80 seconds.
Earlier, Hima had won the gold in the 200m at the Tabor Athletics Meet.
In her first competitive 200m race of the year on July 2, Hima had clocked 23.65 seconds to win gold at the Poznan Athletics Grand Prix in Poland.
Then on July 8, she won her second 200m gold at the Kutno Athletics Meet in Poland. Braving a sore back, the Assam runner had clocked 23.97 seconds to win the gold while Kerala runner VK Vismaya had grabbed silver with a timing of 24.06 seconds.
On July 13, she won her third 200m gold at the Kladno Athletics Meet in Czech Republic with a time of 23.43 seconds.
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domain: sports


Co2 sensor module
Co2 sensor module, also called co2 gas sensor module, is a common electronic device used to detect the concentration of CO2 in the air. With the continuous development of science and technology, the improvement of people’s living standards and people’s increasing emphasis on environmental protection, quantitative monitoring and control of carbon dioxide gas has become a growing demand in air conditioning, agriculture, medical care, automobiles and environmental protection. . Carbon dioxide sensors have a wide range of applications in many fields such as industry, agriculture, national defense, medical and health care, environmental protection, and aerospace.
Type of Co2 sensor module
At present, there are many types of carbon dioxide sensor modules, which can be divided into thermal conductivity type, density meter type, radiation absorption type, conductivity type, chemical absorption type, electrochemical type, chromatography type, mass spectrometry type, infrared optical type, etc. in terms of their principles. : The co2 gas sensor module of electrochemical principle and infrared principle is used more.
Infrared co2 gas sensor module principle
The infrared co2 gas sensor module uses infrared for gas analysis. It is based on the different concentrations of the components to be analyzed, the absorbed radiation energy is different, and the remaining radiation energy causes the temperature in the detector to rise differently, and the two sides of the moving film film are affected The pressure is different, thereby generating an electrical signal of a capacitive detector, so that the concentration of the component to be analyzed can be indirectly measured
According to the different absorption bands of infrared radiation in gas, gas components can be analyzed. For example, carbon dioxide absorbs infrared light with wavelengths of 2.7um, 4.33um and 14.5um quite strongly, and the absorption spectrum is quite wide, that is, there are absorption bands. According to the experimental analysis, only the 4.33um absorption band is not affected by other components in the atmosphere, so this absorption band can be used to determine the CO2 content in the atmosphere.
The sensor uses the principle of non-dispersive infrared (NDIR) to detect CO2 in the air. It has good selectivity and no oxygen dependence. It is widely used in various occasions where flammable and explosive gases exist.
Co2 sensor module is widely used in the following scenarios
Indoor air quality monitoring: CO2 gas sensor module can help monitor indoor CO2 concentration, improve indoor air quality, and prevent the impact of indoor air pollution on human health.
Industrial field: CO2 gas sensor module is often used in the monitoring and control of CO2 concentration in industrial processes, such as CO2 emissions in the production process of steel and cement.


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Contact Be Uplifted Inc
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domain: medical


Blatche working hard to get back in shape for Fiba Asia wars
CEBU CITY—Away from the distractions and sheltered from media and fan intrusions, Gilas Pilipinas continued to push hard in its preparation for the 2015 Fiba Asia men’s basketball championship.
Sacrifice has been the operative word of the national squad, one typified by Andray Blatche, the naturalized NBA veteran whose form has been constantly questioned since rejoining the team.
In one particular drill yesterday, Blatche ran up and down the floor as part of a three-man group, rebounding the ball and passing to a teammate in what was a layup contest among the nationals.
He managed to finish all two rounds of the drill before crumpling in a heap on the sidelines, overcome with exhaustion.
While the Gilas camp was supposed to be off-limits to media people, coach Tab Baldwin allowed interviews with journalists here but asked that Blatche’s weight issues not be discussed.
But the American mentor was more than willing to talk about the other sacrifices made by the team as they chase a lone slot to the Rio de Janeiro Olympics in this year’s continental championship that will be held in Changsa, in China’s Hunan province.
“It’s a really important phase so that’s why we wanted to relocate. And it’s been very tough on these guys,” said Baldwin.
“The guys that are here, they’re not here because their teams allowed them to play. They’re here because they have a passion and that passion takes a toll on them. At the end of all this, they’re going to come back as better players but also diminished athletes because all these takes its toll on their bodies,”
Most of the players in this team have been stars in their PBA ball clubs, but they have readily yielded their superstar status to further bolster team bond.
“Chemistry isn’t really an issue here because nobody here believes that they’re the star,” said veteran shooter Dondon Hontiveros. “We’re all way past that.”
“That’s something that fans find hard to appreciate,” said Baldwin. “It seems glamorous. It seems always exciting but it’s not. It’s a grind and everybody is away from their families. We tend to gloss over that point sometimes and move on to the juicy stuff that people want to talk about but we should never really underestimate the sacrifice these guys make.” Jonas Rey Panerio, Inquirer Visayas


Denmark and Sweden announced on Thursday they will not restart their rollouts of the AstraZeneca vaccine yet, according to statements from their respective health authorities.
The decisions come after Norway also said it wouldn’t restart the use of AstraZeneca for “some time,” while it continues to investigate six cases of severe disease that occurred in the country after vaccination, Steinar Madsen, the Medical Director at the Norwegian Medicines Agency, told CNN’s Richard Quest.
Despite the results of the European Medicines Agency (EMA) investigation on Thursday, which stated that the benefits of the vaccine continue to outweigh the risks, the Danish Medicines Agency said in a statement “it cannot be ruled out that there may be an association between the vaccine and the rare cases of blood clots, a low level of blood platelets and bleeding."
Denmark is expected to hold a press conference on Friday to address the ongoing issue.
Similarly, Sweden will refrain from taking a firm position on the vaccine while it gathers more information, the Public Health Agency said.
“Until then, a continued break is recommended for the use of this vaccine in Sweden," the health authority added.


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I adore Christmas movies. (Feel good movies at this time of the year are the best!) I'm really looking forward to sitting by my tree to watch Angels Sing!
"Harry Connick, Jr. stars as Michael Walker, who, as a child, wished every day was Christmas. That is, until a tragic accident crushed his holiday spirit. Thirty years later, Michael still can’t muster any joy for Christmas, despite encouragement from his playful wife (Connie Britton) and well intentioned parents (Kris Kristofferson and Fionnula Flanagan). But when his young son (Chandler Canterbury) faces a tragedy, Michael needs to make amends with his past. A mysterious man named Nick (Willie Nelson) gives Michael a gift that instills in him the courage to find the Christmas joy that he lost." Perfect holiday viewing! Watch for my review!
Angels Sing is based on the book, When Angels Sing, by Turk Pipkin.
"Writer and actor Turk Pipkin has had a career like no other. A former stand-up comedian who gave Read an excerpt of When Angels Sing.
Here's a quick Q and A with Turk Pipkin!
1. You're a man who has worn many hats - author, actor, comedian, director and activist. And you've met many people in those roles. Who has left their mark on you or impacted your life the most?
---I owe so much to so many, it's hard to choose who's influenced me the most. It's hard to top Desmond Tutu telling me, "The sea is made of drops of water. What you do, where you are, is of significance." Even so, I think Willie Nelson has taught me more than anyone else - in how to respect family, friends and those in need, in being open every day to the possibilities of Love, and in how to make conscious decisions in the way you live your life. In One Peace at a Time, a feature documentary I directed for The Nobelity Project, Willie told me, "The answer is there if you look for it. You know what to do. Right and wrong is not that hard. It’s what you choose to do."
2. Angels Sing is a great movies for families to watch at Christmas. What do you love most about Christmas?
---The thing I like most about Christmas is probably the same as almost everyone else - the way it brings families together; the way it ties them together across generations. That's the core message contained in Angels Sing, and also in my novel that it's based on, When Angels Sing.
3. How can readers support the Nobelity Project?
---The Nobelity Project has made three feature films and fifty short films about global problems and solutions. These are hopeful films about a better way, and I love it when new people find them at www.nobelity.org, on our youtube channel, or any other way. We often have specific fundraising projects for a particular school in Kenya, for tree-planting in the States or for our library and bookmobile project in Honduras, but the engagement almost always comes through our films and photos. So a great place to start is to join the mailing list and like us on Facebook.
4. What's next for Turk Pipkin?
---We built 50 classrooms, libraries and water projects in Kenya last year. We'll do more this year and that works keeps me fairly busy with community engagement, contracting the construction work and reporting on it all. Outside of the Nobelity Project, I'm still writing books and screenplays, doing my best to tell stories that I love, and that I think will have some meaning and some entertainment factor. It's nice when you end up with a movie with Willie Nelson, Connie Britton and Harry Connick, Jr., but you have to remember, it all starts with the story.
Sound like a DVD and book you'd like to enjoy?! Simply leave a comment to be entered. One randomly chosen winner will win a copy of the DVD and book! Open to US and Canada, one entry per person. Ends Dec 21/14.


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with CT11F9 at day 1, 2, 3, 4 or 5 5 post-infection (10 g/g of body weight, single-treatment). myonecrosis. Moreover, thrice-treatment at day 4, Caspofungin Acetate 5, 6 post-infection was associated with an increased survival rate (18.2% single vs. 50% thrice at 20 g/g per body weight), and the mice recovered from limb paralysis. Competitive ELISA also confirmed that CT11F9-acknowledged epitopes were immunodominant in humans. In conclusion, MAb CT11F9 is an ideal candidate to be humanized and used in severe EV71 contamination. Introduction Enterovirus 71 (EV71), belonging to the genus Enterovirus of the family Piconaviridae, is one of the major causative pathogens of hand, Caspofungin Acetate foot and mouth disease (HFMD). Large outbreaks associated with EV71 have been reported worldwide, especially in Asian-Pacific countries, since 1997 C. Although most EV71 infections are self-limited and only cause moderate symptoms, severe complications such as acute flaccid paralysis, encephalitis, pulmonary edema and death have been explained . EV71 has been regarded as the most important neurotropic enterovirus since the eradication of the poliovirus. More than 25 MAbs have been approved for clinical use for diseases such as malignancy, autoimmunity and inflammation, as well as infectious disease . It is possible that MAb may be an ideal therapy option for EV71 contamination. Research has confirmed that a thrice-daily injection of 5.0 mg/mouse of the neutralization polyclonal antibody at 4 h and 1 and 2 days post-infection can fully safeguard mice from EV71-induced death . Neutralization monoclonal antidody (nMAb) also experienced treatment effect: It has been shown that an EV71 MAb belonging to isotype IgM can provide 100% protection when it was administered at 10 g/g of body weigh to 2-week-old AG129 mice, which lack type I and II interferon receptors, one day prior to lethal EV71 challenge . Single-treatment with an EV71-VP1 epitope-targeted nMAb (10 g/g of body weight) at 1 day post-infection was also effective in preventing EV71-induced morbidity and mortality . In our recent research, we have successfully guarded mice from lethal EV71 challenge with an EV71-VP2 targeted nMAb at 1 day post-infection . Although these data provide support for the effectiveness of neutralization antibody in the treatment of EV71 contamination at early occasions of contamination, there is a period of time in the host from the time of viral contamination to the appearance of symptoms, which is a few days in a mouse model . As reported, effective first-treatment time was limited within 24 h post-infection, and less is known about the comparative effect of different first-treatment occasions ranging from contamination to death. In addition, death after onset of EV71-induced illness that rapidly progressed to severe cardiopulmonary failure has been TGFA observed , , , which suggests Caspofungin Acetate that it becomes much harder to treat the infection the closer the first-treatment time was to the onset of illness. Therefore, identifying the suitable time to begin antibody therapy and whether antibody therapy is effective in mice with moderate or severe complications will provide important information as to the treatment potential of MAb to EV71 contamination and will provide guidance for clinical therapeutic usage in EV71 contamination. In a previous study, we characterized a conformational MAb CT11F9 with neutralization activity . In this study, we further characterize the antibody’s neutralization ability and competitive ability to human serum. The antibody’s treatment effect under different treatment occasions and treatment frequencies after EV71 contamination was tested, which highlighted the treatment potential of the nMAb in severe EV71 contamination. Materials and Methods Ethics statement All animal experiments were carried out in accordance with the guidelines of the Xiamen University or college Institutional Committee for the Care and Use of Laboratory Animals and were approved by the Xiamen University or college Laboratory Animal Management Ethics Committee. Written informed consent was obtained from the donor for use of the serum sample. Indie Ethics Committee approval was obtained from the Ethics Committee of the National Institute of Diagnostics and Vaccine Development in infectious diseases. Cells and viruses Human muscular rhabdomyosarcoma (RD) cells were obtained from the American Type Culture Collection (ATCC) and cultivated in Minimal Essential Medium (MEM, GIBCO) supplemented with 10% FBS (GIBCO) plus L-glutamine, penicillin, and streptomycin. Eight EV71 clinical isolates were used (Table 1). Five Taiwan isolates and one prototype strain, BrCr/USA/1970, were sourced from your National Taiwan University or college; one genotype B3 strain, SK-EV006/Malaysia/1997, was sourced from your Tokyo Metropolitan Institute for Neuroscience of Japan; and the EV71/Jiangsu/2008 was isolated in Jiangsu Province. A mouse-adapted computer virus named pSVA-MP4 was generated by four passages in newborn mice using SK-EV006/Malaysia/1997. The EV71 computer virus was loaded onto a 15C50% continuous Caspofungin Acetate sucrose gradient, resulting in fractions with densities at 20C40% after 3 h of ultracentrifugation (32,000g, SW41Ti rotor, Beckman). The fractions were collected, pelleted (100,000g for 2.
Reduced organic killer (NK) function connected with high-risk myelodysplastic syndrome (MDS) and decreased expression of activating NK receptors. 836858 decreases MDSC and Compact disc33+ ANPEP cells in MDS BM specimens A) Scatter properties of MDS BMMNCs sorted with Alexa 488-tagged BI 836858 stained with Cell Tracker Orange and admixed with unstained autologous cells (low risk MDS) and cultured for 4 times 6-O-2-Propyn-1-yl-D-galactose (representative body of n=10). B) Percent 6-O-2-Propyn-1-yl-D-galactose of Compact disc33 positive/Cell Tracker Orange positive cells assessed by stream cytometry. NIHMS840057-dietary supplement-2.tif (2.6M) GUID:?E360B634-55BA-4E48-BF94-7BA3DCAD44AB 3: Supplemental Body 3. BI 836858 blocks downstream induction of Compact disc33-mediated suppressive cytokines MDS BM cells co-cultured with BI 836858, or its particular isotype control, for 96 hours and expression from the cytokines IL-10 (A) and TGF (B and C) was assayed from either supernatants by sandwich ELISA or qPCR respectively. MDS BM cells had been cross-linked with an anti-Fc Fab fragment antibody for fifty percent hour on glaciers before lifestyle for 48 hours of which stage total RNA was gathered for gene appearance evaluation of TGF (D) and Compact disc33 (E). Pubs signify the SEM of three 6-O-2-Propyn-1-yl-D-galactose different experiments assessed in triplicates (ELISA) or duplicates (qPCR). The qPCR data was normalized against the homely home keeping gene GAPDH using the Ct methodology. F) Healthy individual BMMNCs had been pretreated with either nothing at all (control), isotype, “type”:”entrez-nucleotide”,”attrs”:”text”:”BI836858″,”term_id”:”15948408″,”term_text”:”BI836858″BI836858 and Compact disc33Ab accompanied by crosslinking. Lysates were immunoprecipitated with anti-CD33 polyclonal antibody and immunoblotted with SHP1 in that case. Bottom band displays the IgG being a launching control. NIHMS840057-dietary supplement-3.tif (3.3M) GUID:?DF3FC369-35D8-4316-A80E-E5D8501BF6D5 4: Supplemental Figure 4. BI 836858 blocks downstream induction of Compact disc33-mediated ROS A) U937 cells (Compact disc33 positive) had been treated with rhS100A9 and either BI 836858 (correct -panel), or isotype control (still left -panel) for 48 hours before dimension of ROS creation with DCFDA by stream cytometry (representative body). U937 cells (B) or healthful regular PBMC (n=3) (C) had been cultured ex vivo with BI 836858 or isotype control antibody in the existence or lack of rhS100A9 accompanied by stream cytometric evaluation to measure the existence of ROS. D) such as C but using 0 Similarly.5ug/mL LPS stimulation. Antibody-induced adjustments in the percentage of ROS+ cells in healthful PBMC (n=4) or MDS BMMNCs (n=7) was likened after treatment with either 0.5ug/uL LPS (E) or crosslinking using its particular isotype (F). Mistake bars signify the SEM as well as the p worth was computed using Learners T-test. In both F and E the * denotes p 0.05 set alongside the respective healthy counterpart. NIHMS840057-dietary supplement-4.tif (4.4M) GUID:?A11FE99C-856D-4E94-B3CA-E558078DC4Compact disc 5: Supplemental Body 5. BI 836858 6-O-2-Propyn-1-yl-D-galactose prevents the introduction of S100A9/Compact disc33-mediated genomic instability Healthful regular PBMC (n=3) (A) had been cultured ex girlfriend or boyfriend vivo with isotype control or BI 836858 antibody in the existence or lack of rhS100A9 accompanied by comet evaluation to measure the protective aftereffect of BI 836858 against S100A9-induced DNA harm. 6-O-2-Propyn-1-yl-D-galactose Fifty images each from three principal specimens had been analyzed. (B) Consultant images of tail momentum for PBMC and HSPC from MDS BM. (C) Lineage-CD34+ HSPC in MDS BMNC had been measured by stream cytometry for the current presence of H2AX activation after treatment with BI 836858, Compact disc33Ab or their particular isotypes before and after crosslinking as defined before. Representative body is proven. NIHMS840057-dietary supplement-5.tif (2.3M) GUID:?B9F7FF3D-FAB4-4F11-96A4-A67959E50E2A Abstract We recently reported the fact that accumulation of myeloid-derived suppressor cells (MDSC), thought as Compact disc33+HLA-DR?Lin?, has a direct function in the pathogenesis of myelodysplastic symptoms (MDS). Specifically, Compact disc33 is expressed in MDSC isolated strongly.
Daugaard, P. 5% of bronchitis and sinusitis in adults and children (22, 33, 40, 41, 52). Seroepidemiology has shown that most infections are asymptomatic. Regional and international serology-based epidemiologic studies of have shown high prevalences and ubiquitous contamination. These studies have indicated that most persons have had their first contamination before age 20 and commonly become reinfected (1). The biphasic life cycle of and as well as their intracellular host cell parasitism could allow for maintenance of a chronic infection. For example, can persist in mammals and birds lifelong and only occasionally cause disease, most often after induction of stress (23). has been demonstrated to multiply in macrophages, endothelia, and clean muscle cells in vitro (13, 26), and this multiplication has been associated with cytokine production and induction of adhesions (10, 24, 25). However, demonstration of a state of chronic contamination has been more elusive. Many laboratory methods have been developed for the diagnosis of contamination, including primary isolation of the organism in cell culture, serologic assays, immunohistochemical assays, and PCR (17). Despite great effort to improve primary culture techniques of contamination. Serologic assays include complement fixation, microimmunofluorescence (MIF), enzyme-linked immunosorbent assay (ELISA), and immunohistochemistry (2). Each of these assays requires significant technical expertise and is subject to investigator interpretation. The MIF test remains the most sensitive, species-specific assay and is the gold standard for determining the prevalence of in study populations (45, 46). The traditional MIF assay relies on the use of whole EBs as antigens. Though lacking the necessary species specificity for use as a diagnostic serologic test, indirect immunofluorescence assay (IFA) has been used for culture confirmation of isolates or for laboratory culture standardization. IFA relies on both whole RBs and EBs fixed with methanol IX 207-887 as antigens in antigen has been observed by investigators to have cross-reactivity in certain serologic and immunohistochemical assessments (4). Standardized assays that reduce the requirements for highly specialized, well-trained personnel while still providing species specificity are greatly needed for further investigations into the natural history and epidemiology of CWL 029 (ATCC VR-1310) as the immunogen. The primary 8A6 MAb-producing cells were cultured in Iscove’s altered Dulbecco’s medium (Gibco BRL, Rockville, Md.) supplemented with 10% low-immunoglobulin G (IgG) fetal bovine serum (HyClone, Ogden, Utah). Clone supernatants were assayed for reactivity to by IFA (see below). Reactive wells were further subcloned by limiting dilution analysis with methods previously described (49). Subcloning was repeated two more times to ensure that the most-reactive IX 207-887 single-cell clones were produced. Clones resulting from single-cell selection were expanded and screened as described previously (49). The clone producing the highest concentration of reactive IX 207-887 MAb (based on IFA testing of clones) was then weaned onto BD cell medium (BD Pharmingen, San Diego, Calif.), according to the manufacturer’s protocol, for generating a large-scale antibody. The 8A6 MAb-producing cell line’s growth and MAb production were scaled up by using Mouse monoclonal to CD16.COC16 reacts with human CD16, a 50-65 kDa Fcg receptor IIIa (FcgRIII), expressed on NK cells, monocytes/macrophages and granulocytes. It is a human NK cell associated antigen. CD16 is a low affinity receptor for IgG which functions in phagocytosis and ADCC, as well as in signal transduction and NK cell activation. The CD16 blocks the binding of soluble immune complexes to granulocytes BD cell medium. The cells were incubated in roller bottles at 37C in a 10% CO2 humidified chamber for an additional 12 days. Cells were collected by centrifugation, and the medium was carefully harvested without disrupting the cell pellet. Reactivity and species specificity of the MAbs selected were decided using indirect IFA.
(A) Fluorescent micrographs of filipin staining (blue) in Npc1+/+, Npc1+/? and Npc1?/? astrocytes. scale). Averaged data had been extracted from at least three unbiased experiments. Each worth corresponds towards the indicate S.E. of 20 cells within a consultant of three tests.(TIF) pone.0071361.s001.tif (10M) GUID:?86A0CCompact disc1-DCD6-4960-A5Compact disc-7AC1Compact disc3473E0 Figure S2: Cholesterol accumulation partially mimics the increased dye uptake of Npc1?/? astrocytes. (A) Fluorescent micrographs of filipin staining (blue) in Npc1+/+, Npc1+/? and Npc1?/? astrocytes. Also proven fluorescent micrographs of filipin staining in Npc1+/+ astrocytes subjected to automobile (EtOh) or treated with 0.5 or 1 g/ml U1866A for 24 or 48 h. Calibration club?=?25 m. (B-C) Averaged data (normalized to regulate; dashed series) from the price of Etd uptake by Npc1+/+ astrocytes subjected to automobile or 0.5 and 1 g/ml U1866A for 24 (B) or 48 h (C). Additionally, the result of 200 M La3+ applied during Etd uptake experiments is shown acutely. *p 0.05 set alongside the basal degree of Npc1+/+ astrocytes. The averaged data had been extracted from four unbiased tests.(TIF) pone.0071361.s002.tif (32M) GUID:?D7A51BD4-89D9-447C-9EC5-80A3338EBFBD Film S1: Time-lapse (4 min and 30 s) movie of spontaneous adjustments in the Fura-2 proportion (340/380 proportion, pseudo-colored scale) in Npc1+/+ and Npc1?/? astrocytes (still left and correct, respectively). Structures in the film aside had been captured 3 s.(AVI) pone.0071361.s003.avi (1.6M) GUID:?5E043C9D-EEA4-46BD-99A3-1D119826841B Abstract Reduced astrocytic difference junctional communication and improved hemichannel activity were recently proven to boost astroglial and neuronal vulnerability to neuroinflammation. Furthermore, increasing evidence shows that neuroinflammation has a pivotal function in the introduction of Niemann-Pick type C (NPC) disease, an autosomal lethal neurodegenerative disorder that’s due to mutations in the gene mainly. Therefore, we looked into whether the insufficient NPC1 appearance in murine astrocytes impacts the functional condition of difference junction stations and hemichannels. Cultured cortical astrocytes of NPC1 knock-out mice (Npc1?/?) demonstrated reduced intercellular conversation via difference junctions and elevated hemichannel activity. Likewise, astrocytes of newborn Npc1?/? hippocampal pieces provided high hemichannel activity, that was totally abrogated by connexin 43 hemichannel blockers and was resistant to inhibitors of pannexin 1 hemichannels. Npc1?/? astrocytes also demonstrated even more intracellular Ca2+ indication oscillations mediated by useful connexin 43 hemichannels and P2Y1 receptors. As a result, Npc1?/? astrocytes present top features of connexin structured channels appropriate for those of reactive astrocytes and hemichannels may be a book therapeutic target to lessen neuroinflammation in NPC disease. Launch Niemann-Pick type C (NPC) disease can be an autosomal recessive neurodegenerative disorder that’s due to mutations in the or genes . Most situations of NPC disease are due to mutations in the gene that produces a dysfunctional proteins , . NPC1 and NPC2 protein are necessary for the trafficking of cholesterol; therefore, a lack of function in these protein leads to the intracellular deposition of free of charge cholesterol and various other lipids in past due endosomes/lysosomes . Intensifying neurodegeneration, hepatosplenomegaly, and dysfunction 6-Maleimido-1-hexanol of various other organs are found in 6-Maleimido-1-hexanol sufferers affected with NPC disease . These symptoms are found within a murine style of NPC disease  also, 6-Maleimido-1-hexanol . Npc1?/? mice present cortical and 6-Maleimido-1-hexanol hippocampal neuronal dysfunction C, apoptosis of Purkinje neurons from the cerebellum and neuronal loss of life in different human brain locations C. Astrocytes exhibit NPC1; and in the Npc1?/? mouse human brain, Npc1?/? astrocytes display morphological changes and be turned on , . The global neuronal deletion of NPC1, however, Sema3b not astrocyte-specific NPC1 insufficiency, leads to the entire advancement of NPC neuropathology , which implies that neuronal NPC1 insufficiency is enough to mediate neurodegeneration. Nevertheless, rescuing NPC1 expression in astrocytes delays 6-Maleimido-1-hexanol neuronal loss and prolongs the entire life time in Npc1?/? mice , recommending that astrocytes might enjoy a significant role in the neuroinflammatory condition of NPC disease. Neuroinflammation exists in Npc1?/? mouse human brain at an early on post-natal age and it is characterized by a sophisticated variety of microglia, elevated degrees of interleukin-1 and.
D., D. at months 0 and 6 to girls aged 9C14 years; 2D (M0,12), 2-dose schedule administered at months 0 and 12 to girls aged 9C14 years; 3D (M0,1,6), 3-dose schedule administered at months 0, 1, and 6 to women aged 15C25 years; ED50, effective dose producing 50% response; ELISA, enzyme-linked immunosorbent assay; EU, ELISA models; GMT, geometric mean antibody titer; PBNA, pseudovirion-based neutralization assay. Open in a separate window Physique 3. Human papillomavirus (HPV)18 immune responses for initially seronegative subjects in the month 12/13 according-to-protocol immunogenicity cohort. Bars represent GMTs and associated 95% confidence intervals; numbers within each bar are the GMTs for each group; initially seronegative subjects were those who had an antibody titer lower than the assay cutoff (7 EU/mL for ELISA; 40 ED50 for PBNA). Box plots show median, lower and upper quartiles, and minimum and maximum values; initially seronegative subjects were those who were seronegative at ELISA. Natural Contamination represents HPV-18 GMT measured with ELISA for women aged 15C25 years who had cleared a natural contamination in Study HPV-008 (22.6 EU/mL)  or with PBNA for women aged 18C45 years who had cleared a natural infection in Study HPV-010 (137.3 ED50) ; plateau, HPV-18 GMT measured with ELISA at month 45C50, which was 297.3 (258.2 to 342.2) EU/mL for women aged 15C25 years in the total vaccinated cohort from Study HPV-007 . Abbreviations: 2D (M0,6), 2-dose schedule administered at months 0 and 6 to girls aged 9C14 years; 2D (M0,12), 2-dose schedule administered Melanotan II at months 0 and 12 to girls aged 9C14 years; 3D (M0,1,6), 3-dose schedule administered at months 0, 1, and 6 to women aged 15C25 years; ED50, effective dose producing 50% response; ELISA, enzyme-linked immunosorbent assay; EU, ELISA models; GMT, geometric mean antibody titer; PBNA, pseudovirion-based neutralization assay. Comparable results were observed in the ATP-I and TVC regardless of baseline serostatus (Supplementary Tables 2C9). In the ATP-I, geometric mean avidity indices (95% CI) 1 month after the last vaccine dose for HPV-16 and HPV-18, respectively, were 92.8% (89.8%C96.0%) and 84.8% (81.8%C88.0%) for the 3D group and 88.8% (86.9%C90.9%) and 89.6% (86.9%C92.3%) for the 2D (M0,6) group (Supplementary Physique 2). Among low antibody responders (in the lowest decile for GMTs 1 month after the last dose), HPV-16/18 GMTs seemed higher in 2D groups than in the 3D group (Supplementary Table 10). Cross-reactive Immune Responses to Nonvaccine HPV-31 and HPV-45 At month 7, cross-reactive HPV-31/45 antibody and CMI responses were of comparable magnitude in girls aged 9C14 years who received 2D (M0,6) Melanotan II and women aged 15C25 years who received 3D (Supplementary Physique 3). There was large variability in HPV-31C and HPV-45Cspecific memory B-cell responses, but median EBR2A values were within a similar range in the 2D and 3D groups. Similar results were observed in the ATP-I and TVC regardless of baseline immune status (Supplementary Tables 2C9). Reactogenicity and Safety The incidence of local and general solicited symptoms overall per subject, during the 7-day period after each dose, is shown Melanotan II in Figure ?Physique44 and Supplementary Table 11. Incidence overall per dose is usually shown in Supplementary Table 12. Pain at the injection site was the most frequently solicited local symptom (reported by 90% of subjects in each group). The incidence of grade 3 pain ranged from 9%C12% across groups. Fatigue (45%C65% of subjects), myalgia (51%C62%), and headache (37%C51%) were the.
A direct comparison of reported data is difficult due to a variety of reasons. system were considered the most critical for the risk assessment. From a human study, a lowest BMDL 10 of 17.5 ng/mL for the sum of the four PFASs in serum was identified for 1\year\old children. Using PBPK modelling, this serum level of 17.5 ng/mL in children was estimated to correspond to long\term maternal exposure of 0.63 ng/kg bw per day. Since accumulation over Soblidotin time is important, a tolerable weekly intake (TWI) of 4.4 ng/kg bw per week was established. This TWI also protects against other potential adverse effects observed in humans. Based on the estimated LB exposure, but also reported serum levels, the CONTAM Panel?concluded that parts of the European population Soblidotin exceed this TWI, which is of concern. and studies on PFOS and PFOA suggest that immunotoxic effects may originate from modulation of PPARs, NF\B regulated gene transactivation and/or regulation of apoptosis. The MOA behind the impaired mammary gland development in mice dosed with PFOA during gestation and neonatally is unknown. Considering critical effects, in human studies, various associations between serum levels and a number of Mouse monoclonal to IgM Isotype Control.This can be used as a mouse IgM isotype control in flow cytometry and other applications outcomes have been reported. In the previous Opinion (EFSA CONTAM Panel, 2018), four endpoints were selected as potential critical effects for PFOS and/or PFOA. These were (i) increased serum total and LDL cholesterol (risk factor for cardiovascular disease), (ii) increased ALT levels (indicating effects on liver cells), (iii) reduced birth weight and (iv) effects on the immune system as shown by decreased antibody response to vaccines. In 2018, the CONTAM Panel?used the effects on serum cholesterol levels to derive TWIs for both PFOS and PFOA. Those TWIs were also protecting towards the other potential critical endpoints. Although the association with increased cholesterol was observed in a large number of studies, the CONTAM panel now considers the uncertainty regarding causality to be larger. This is primarily due to a postulated biological process around the enterohepatic cycling of both PFASs and bile acids, the latter affecting serum cholesterol levels. This should be further investigated. For increased ALT, the CONTAM Panel?concluded that more studies are needed to support the causality of the effect. Concerning reduced birth weight, a recent study seems to strengthen the causality. However, as concluded in 2018, the decrease in birth weight after adjusting for confounders Soblidotin is not large and the potential longer term consequences of this decrease are unclear. The CONTAM Panel?concluded that effects on the immune system, which were observed at the lowest serum PFAS levels in both animals and humans, are critical for the risk assessment. The findings of a decreased immune response were considered robust since they were consistently observed for the two studied PFASs in rodents (PFOA, PFOS) and in humans. The CONTAM Panel?noted that this is not the case for effects on mammary gland development, which are observed at similar low serum levels in mice but have not been studied in Soblidotin other animal models or humans. Therefore, the CONTAM Panel?decided to base the present assessment on PFASs on effects on the immune system. Based on observations in animals and humans, the CONTAM Panel?decided to combine its assessment on the sum of four PFASs, i.e. PFOA, PFNA, PFHxS and PFOS. At.
Cross-reactive Dengue virus (DENV) and Western Nile virus antibodies have been proven to enhance ZIKV pathogenesis [9,10,11]. which the effect of YFV post-vaccination antibodies on ZIKV replication was cell line-dependent. Embryoid physiques had been permissive to ZIKV also, and the current presence of YFV antibodies gathered 4C14 weeks post-vaccination decreased ZIKV disease when placental cells had been present. Nevertheless, when contaminated with ZIKV straight, the embryoid physiques displayed significantly improved viral lots in the current presence of YFV antiserum used thirty days post-vaccination. The info show that every from the cell lines and EBs possess a distinctive response to ZIKV complexed with post-vaccination serum, recommending there could be cell-specific systems that effect congenital ZIKV attacks. Since ZIKV attacks can cause serious congenital syndromes, it is very important to comprehend any potential safety or improvement provided from cross-reactive, post-vaccination antibodies. mosquitoes. YFV and ZIKV started in Africa and also have been discovered to co-circulate inside the same parts of Latin America . ZIKV made an appearance in the Traditional western Hemisphere in 2015 [2 1st,3]. YFV, nevertheless, continues to be circulating in the Americas because the African slave trade period and it is endemic in lots of tropical regions such as for example Brazil, Columbia, Venezuela, and Peru to mention several . In the 1930s, a live attenuated vaccine for YFV, 17D, originated and, in its nearly 80 years useful, has which can have a substantial impact on managing YFV outbreaks [4,5]. Multiple countries possess mass vaccination applications, and some national countries, where YFV can be endemic, possess the YFV-17D vaccine contained in the nationwide recommended years as a child immunization schedule. Especially, Bolivia, Brazil, Columbia, Ecuador, and Venezuela all recommend the vaccine to kids 9C12 months old within the complete country, not really in known endemic regions  simply. Despite these suggestions, recent surveys demonstrated that bit more SB366791 than fifty percent of the populace in these areas are vaccinated for YFV SB366791 . Using the ongoing vaccination promotions in these certain specific areas, there are always a spectral range of post-vaccination YFV antibodies, a few of which might improve infections by additional flaviviruses. Numerous flaviviruses co-circulating in the same areas in Southern and Central America, there may be the chance for antigenic SB366791 cross-reactivity, specifically since some YFV-endemic areas possess reported seroprevalence prices of ZIKV up to 63% [4,8]. Antigenic cross-reactivity and antibody-mediated enhancement occur between flaviviruses. Cross-reactive Dengue disease (DENV) and Western Nile disease antibodies have been proven to enhance ZIKV pathogenesis [9,10,11]. Nevertheless, only limited research have already been conducted for the potential cross-reactive character of YFV antibodies. One research, using industrial ELISA recognition products for ZIKV and DENV, discovered there to become minimal cross-reactivity between YFV DENV and antibodies recognition, no cross-reactivity in ZIKV recognition [12,13]. While these scholarly research had been extremely educational, they didn’t represent the real immunological panorama, as Souza et al.  utilized post-vaccination serum from 9-month-old babies, who’ve an undeveloped disease fighting capability, as well as the CDC MAC-ELISA for ZIKV was validated utilizing a test size of less than 10 people, of an unfamiliar exposure background . Furthermore, SOUTH USA, especially Brazil, includes a high occurrence of measles, that may affect immunological memory space in recovered individuals . This, nevertheless, does not reveal feasible in vivo relationships, as many reviews indicate that flaviviral neutralization would depend CDH2 and complicated upon many SB366791 factors . It’s been demonstrated that antibodies that neutralize in vitro also, such as for example in neutralization assays, usually do not neutralize in vivo frequently, suggesting that complicated immunological interactions happen for neutralization [16,17,18]. In areas where ZIKV includes a high prevalence, a big part of the populace offers YFV antibodies, not only through the childhood plan of immunizations but also from ongoing vaccination promotions that inoculate adults and offer boosters for women that are pregnant, HIV-infected individuals, and additional immunocompromised populations . Having a spectral range of YFV antibodies within this population, it’s important to comprehend any feasible cross-reactivity, antibody-mediated enhancement, or antibody-mediated neutralization. Research have reported how SB366791 the vaccination of women that are pregnant with YFV happens during vaccination promotions [20,21]. While many studies show that vaccination with YFV during being pregnant can be safe, the introduction of protective.
Sutton P. anti-N-terminal replies were more frequent, of higher titers, and of cytophilic subclasses primarily. Comparing antibody replies to different PfMSP3 variations using the genotype present during an infection demonstrated that anti-N-terminal replies were generally allele class particular, Sulfasalazine but there is some proof for replies that cross-reacted across allele classes. Proof for cross-reactive replies was stronger when variations within one allele course were tested, which includes implications for the logical advancement of genotype-transcending PfMSP3-structured vaccines. INTRODUCTION Your time and effort to build up a vaccine concentrating on bloodstream stage parasites, that are in charge of all malaria-related fatalities worldwide practically, continues to be notably influenced by two latest phase Sulfasalazine IIb studies which didn’t bring about detectable security (21, 27). While unsatisfactory, these results experienced the beneficial influence of triggering comprehensive debate of how vaccine applicants are chosen and what data are essential to rationally progress them along the vaccine advancement pipeline (3, 7, 10). These analyses obviously identify genetic variety among the most significant complications in vaccine advancement. Bloodstream stage vaccine applicants are of particular concern upon this score, because they are subjected to the adaptive disease fighting capability, a solid selective pressure that may drive genetic variety (36). Certainly, many bloodstream stage antigens seem to be under controlling selective pressure, recommending that immune system replies to them are generally allele specific which multiple allelic variations cocirculate within confirmed parasite people (18, 38). Immunoepidemiology research have already been an useful device in the malaria vaccine advancement procedure extremely. Nevertheless, as the vaccine advancement process moves forwards, there can be an urgent dependence on these research to deal with the issue of genetic variety and allele-specific immune system responses at once. Allele-specific replies are discovered using antibody depletion tests often, where antibodies that acknowledge one antigen are depleted from a serum test by multiple incubations with this antigen prior to the existence of antibodies that acknowledge a different antigen variant is normally detected. Such research have already been effective incredibly, with essential implications for vaccine applicants such as for example AMA1 (23, 24), MSP1 (14, 34), and MSP3 (4, 22, 25). However, they are not usually possible in all studies because of sample volume limitations, particularly when multiple different variants of each antigen are used in individual competition experiments on the same serum sample. An Sulfasalazine alternative and simple method to detect allele-specific immune responses would be to directly compare the immune response to multiple antigen genotypes with that of the antigen genotype present in the infection from which the sample has been taken. If an individual serum sample contained antibodies that acknowledged only the infecting allele type, this would be a strong argument for allele specificity. Such an approach is clearly difficult in hyperendemic transmission environments, where individuals are routinely infected with multiple overlapping genotypes. In contrast, in hypoendemic environments, where infections are genetically RAB25 simple and often spaced by several months, the responses against both the infecting and noninfecting genotypes could be reasonably compared, and a direct correlation between genetic variation and the immune response could be inferred. In the context of a longitudinal study, where the contamination history of each individual is known for an extended period of Sulfasalazine time and hence the length of time since they had been exposed to other allelic types is established, that comparison would be even more powerful. To test the validity of this approach, we used samples from a longitudinal epidemiological cohort near Iquitos, Peru (1), and investigated responses against merozoite surface protein 3 (PfMSP3). PfMSP3 is usually encoded by one member of a multigene family (30), is usually expressed on the surface of merozoites (16, 19), and consists of two major domains, a polymorphic N-terminal domain name and a relatively conserved C-terminal domain name (11, 15). Genetic diversity within the N-terminal domain name consists of sequence polymorphisms and multiple indel mutations, which define two allele classes termed 3D7 and K1 (6, 11). Antibodies targeting PfMSP3 are associated with long-term clinical protection, and full-length PfMSP3 provides strong protection against homologous challenge in an monkey model (8, 26). To date, PfMSP3 vaccine efforts have so far focused almost exclusively around the C-terminal domain name, both because it is usually highly conserved and because specific subregions of the C-terminal domain name can generate protective immune responses (5, 13, 17, 29, 32). However, anti-PfMSP3 N-terminal domain name antibodies are also able to elicit protective responses (28, 29). As a proof-of-concept study to test the approach of using sera from genotyped infections in.
The methodology and panel described here can support the extensive immunophenotyping of cancer FFPE tissues. created an optimized workflow for optimum antibody efficiency by separating antibodies into two specific incubation steps, at different incubation and temperature ranges moments, proven to improve immunodetection significantly. Furthermore, we offer insight in to p-Synephrine the antibody validation procedure and discuss why some antibodies and/or mobile markers aren’t appropriate for the technique. This function is targeted at helping the execution of imaging mass cytometry in various other laboratories by explaining methodological procedures at length. Furthermore, the -panel described here’s an excellent immune system monitoring tool that may be easily used in the framework of tumor research. strong course=”kwd-title” Keywords: imaging mass cytometry, tumor microenvironment, immunophenotyping, CyTOF, tumor immunity, immunotherapy Launch Technology that support the high dimensional evaluation of natural systems are crucial in scientific analysis and also have become significantly relevant in scientific contexts. For example, the development of T cell checkpoint blockade remedies for tumor treatment provides revitalized the field of tumor immunotherapy but also released an urgent dependence on the breakthrough of biomarkers that information individual selection for remedies (1, 2). Furthermore, latest works utilizing single-cell platforms predicated on RNA sequencing and mass cytometry possess delivered an abundance of data uncovering previously unappreciated cell subsets and book functionalities (3C5). Even so, most p-Synephrine immunophenotyping methods are held back again by having less spatial resolution, restrictions in the amount of goals that may be concurrently visualized, or troublesome protocols. Methodologies such as for example movement cytometry may be employed to investigate multiple markers but are inadequate to graph the vast spectral range of immune system cells within an impartial manner (6). Single-cell mass cytometry overcomes this limitation by allowing the simultaneous evaluation of ~40 mobile markers currently. However, it does not have spatial details also, failing woefully to reveal tissues context and mobile interactions which are really relevant in physiological and disease expresses (7C9). Conversely, multispectral fluorescence imaging provides spatial framework but is bound to few markers and it is hence best suited to research specific research queries in huge cohorts (10, 11). The latest introduction of imaging mass cytometry provides advanced the to concurrently get details on phenotypes significantly, their localization within a tissues, also to map mobile connections. Mass cytometry employs steel isotopes conjugated to antibodies appealing, as opposed to movement p-Synephrine immunofluorescence and cytometry methods that depend on fluorescent dyes. The steel isotopes are recognized by mass within a time-of-flight mass spectrometer and, hence, the amount of markers that may be discovered isn’t tied to spectral overlap simultaneously. Since its breakthrough in ’09 2009 (12), mass cytometry continues to be requested the immunophenotyping of tumor microenvironments successfully. It has accelerated the breakthrough of new immune system cell subsets, the evaluation of potential relationship and biomarkers of immune-phenotypical adjustments to healing final results (5, 13C15). Imaging mass cytometry employs a high quality laser that’s coupled towards the mass cytometer (16). Successive ablations of little portions of tissues (~1 m2) are examined by CyTOF (Cytometry Time-Of-Flight) thus quantifying p-Synephrine the current presence of steel isotopes per section of tissues. This data is certainly reconstructed into an artificial multilayer picture producing a wide and comprehensive summary of proteins appearance em in situ /em . Imaging mass cytometry may be employed for imaging up to 40 markers in various tissues resources (e.g., snap-frozen, FFPE), however the combination of a lot of antibodies in the same test raises methodological problems: (1) The tests and validation of a lot of antibodies can be an onerous and labor-intensive procedure. (2) The decision of tissues source must consider the option of antibodies aimed against indigenous or denatured antigen conformations. Furthermore, the usage of FFPE requires that antibodies function beneath the same antigen retrieval circumstances. (3) The perfect immunodetection circumstances are adjustable for different antibodies. By merging 40 antibodies into one test an optimized workflow should be designed to be able to get best antibody efficiency. We created a 40 marker -panel for the evaluation of FFPE tissue by imaging mass cytometry. Next to a great deal of lineage and useful immune system cell Rabbit Polyclonal to 60S Ribosomal Protein L10 markers, the -panel also includes surrogates of tumor cell expresses (e.g., proliferation, apoptosis) and structural markers (e.g., epithelium, stroma, vessels) for a thorough overview of tumor immune system microenvironments but also to research cancer-immune cell connections. Furthermore, we developed an optimized immunodetection process where antibodies are put into two incubation guidelines, thus reducing the focus of total antibody per working-solution and using the.
Since the RAG proteins are only expressed at early stages of lymphoid development, assessing their activity is challenging. in immune homeostasis. Here, we will discuss recent improvements in the mechanisms underlying the pathophysiology of the immune dysregulation associated with hypomorphic mutations in patients and in animal models. Molecular and biochemical structure of human RAG1 and RAG2 The generation of an extensive repertoire of immunoglobulin and T cell receptor (TCR) molecules in developing lymphocytes is usually ensured by the combinatorial association of dispersed variable (and gene segments made up of conserved consensus nonamer and heptamer elements separated by a degenerate spacer of either 12 or 23 nucleotides are recognized by the Recombination activating gene proteins, RAG1 and RAG2 (5). Expression of RAG genes is usually tightly regulated Dodecanoylcarnitine and occurs at Rabbit Polyclonal to MYB-A early stages of T and B cell differentiation. The RAG proteins form a heterotetramer with two subunits each of RAG1 and RAG2, that recognizes and Dodecanoylcarnitine binds to Dodecanoylcarnitine a pair of RSSs, introducing a DNA double strand break at the junction with the coding gene segment. Efficient recombination occurs only when the RAGs bind one 12RSS and one 23RSS (the so called 12/23 rule). However, in the rearrangement of T-cell receptor beta and delta loci, joining of and gene segments bordered by the 23 and 12 RSS does not occur, and an intervening segment has to be joined to before a segment can be joined to the rearranged product (the so-called beyond 12/23 restriction) (6, 7). The human and genes are located in a tail to tail configuration on chromosome 11p13 and are separated by only 8 kb (8). Both the genomic organization of the genes and the amino acid composition of the RAG proteins are highly conserved throughout development. Furthermore, the observation that RAG proteins share similarities with numerous transposases and can mediate transposition (9, 10), supports the hypothesis that RAG recombinase originates from a common transponsable element that joined the genome of a common ancestor of all jawed vertebrate. Consistent with this hypothesis, the transposon superfamily has been recently recognized in the genome of the basal chordate amphioux (11C13). Multiple levels of regulation of gene expression have been hypothesized to occur because of the on-off fluctuation observed during lymphocyte development. Furthermore, expression of the RAG proteins is also regulated at the post-translational level. and data Dodecanoylcarnitine indicate the presence of cis-regulatory elements in the locus, and an additional regulatory mechanism has been explained to mediate the regulated degradation of the RAG2 protein via phosphorylation at threonine 490 (T490) and targeting to the ubiquitin-proteosomal pathway (8). Structural studies have recently exhibited the architecture of the core RAG heterotetramer. Binding of a RAG1/RAG2 heterotetramer together with the high mobility complex groups (HMGB1 or HMGB2) to one RSS and synapsis with a partner RSS results in introduction of a nick on one DNA strand between the heptamer and the flanking coding element during the G0/G1 phase of the cell cycle, with generation of cleavage paired complex. Subsequently, a transesterification reaction occurs, with formation of sealed hairpin coding ends and RSS-containing blunted signals ends, to which the RAG heterotetramer remains bound in a cleaved transmission complex. Upon ARTEMIS activation by the DNA-dependent protein kinase catalytic subunit (DNA-PKcs), opening of the hairpins occurs, and both coding and transmission ends are processed by proteins of the nonhomologous end joining (NHEJ) pathway, leading to the joining of the two coding ends and formation of a circular DNA product containing the transmission ends (14, 15). During the joining process, asymmetrical opening of the hairpin by ARTEMIS may allow incorporation of palindromic sequences (P-nucleotides), and terminal deoxynucleotidyl transferase (TdT) may expose additional nucleotides (N-nucleotides) in the coding sequence. Structure Dodecanoylcarnitine of the human RAG 1 and RAG2 proteins The RAG1 and RAG2 protein sequences are not related to each other. The human RAG1 protein is composed of 1043 amino acids. The N-terminal region of the protein has been implicated in non-specific interactions and involved in ubiquitylation-dependent regulatory.

domain: biology


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| Nov 4, 2011
| 3:10 PM
As I write this, I could swear I hear celebrity TV chef Gordon Ramsay's voice bellowing down 36th Street from Café Hon, 1002 W. 36th St. The host of Fox's "Kitchen Nightmares" is in town with a crew to give the longstanding Hampden eatery a going over for...
| Nov 15, 2011
| 1:18 PM
COLUMBUS, Ohio — If you're hungry for Japanese food and wondering where to go in this hemisphere, you are likely to think of Los Angeles, New York or Honolulu. On second thought, you might add San Francisco, Chicago, Toronto or Vancouver, British...
| Aug 11, 2012
Would you rather be loved or respected? The Mo's group of seafood restaurants and markets gets a lot of the former and not much of the latter. When Anthony Bourdain chose Mo's to represent Baltimore dining on a 2009 "No Reservations" episode, Baltimore...
| Aug 21, 2012
| 9:06 AM
Anthony Bourdain is returning to the Hippodrome on Nov. 17.
Bourdain, chef-at-large at New York's famed Brasserie Les Halles, is the author of best-sellers "Kitchen Confidential" and "Medium Raw" — blunt and sometimes shocking portraits of life...
| Aug 27, 2012
| 8:21 AM
The fall TV season gets underway in September, with the brand new season of "Doctor Who" leading the way. This year the major networks have spread out their new and returning show premieres. If you don't see a series listed here, it's more than likely not...
| Mar 19, 2013
The cuisine and restaurants of Baltimore are getting another turn in the national spotlight.
The March 25 episode of "Bizarre Foods America with Andrew Zimmern" features Baltimore and the Chesapeake Bay.
The last time Baltimore was the subject of a...
| Oct 18, 2012
| 4:44 AM
The very last episode of Anthony Bourdain's "No Reservations" will offer a taste of both New York and Baltimore.
The Baltimore flavor will come by way of "The Wire."
As the Los Angeles Times puts it, Bourdain has frequently "geeked out" about "The...
| Nov 13, 2012
| 12:46 PM
When Anthony Bourdain brings his Guts & Glory tour to the Hippodrome on Saturday, it will be a return engagement for the notoriously opinionated "chef-at-large".
Bourdain launched the Hippodrome's annual Foodie Experience series in May 2010, when he...
| Nov 19, 2012
| 9:07 AM
Anthony Bourdain brought his "Guts and Glory" tour to Baltimore's Hippodrome Theatre on Saturday night. In typical Bourdain fashion, he began his performance with a round of shots.
"Guts and Glory" is essentially stand-up comedy about Bourdain's...
| Mar 28, 2012
| 7:29 AM
Spring is showering new and returning TV series, movies and specials upon viewers. Here's a calendar of upcoming shows. As is always the case, I will update as I learn about more.
Sunday, April 1
10 a.m. | "Ultimate Spider-Man," Disney XD (series...
| Jun 20, 2013
| 12:58 PM
Many Baltimoreans declare that their morning commute only becomes bearable when tuned into the crazy antics of WWMX-FM morning radio host Reagan Warfield. He regularly interviews world-famous celebrities and rock stars and banters with a widespread...

domain: entertainment


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Organizational change: Organizations undergo changes due to new technological, political and social developments that affect them or due to changes in the competitive force. As people may hold different view about the change and the future direction of an organization, conflicts may arise (Mullins).
Personality Clashes: Individuals' personalities differ widely due to differences in their levels of maturity, emotional stability and their behaviour. When they do not recognize or appreciate these differences, conflicts occur.
Differences in value sets: Different people have different opinions, values and beliefs. When people with contradicting values and beliefs interact with each other, conflicts are likely to occur. These conflicts are often irrational and difficult to be resolved (Newstorm).
Threats to status: Most individuals associate their identity with their status in society or the organizations. When an individual feels that another person's acts may harm or damage his image, which in turn may affect his status, conflict is likely to arise.
Differences in Perceptions: People perceive different things, issues and their environment differently. When they act as though their perception is the only realty, without attempting to understand or accept another person's view, conflicts arise (Newstorm).
Classification of Conflicts
Conflicts are common in all organizations and occur when people fail to arrive at a consensus regarding the organizational goals or the means to achieve them (Falletta). Organizational conflicts can be classified as follows:
According to most behavioural theories, people are motivated to achieve a goal either when meeting the goal results in the satisfaction of a need, or when the incentives for achieving the goal are attractive. However, this is not as simple as it seems. An individual may have conflicting needs or he may have to overcome many barriers to achieve his goals. Thus, the process of achieving goals is a complicated one. In the process of achieving his gaols, an individual may experience stress and frustration and may face internal conflict. This is referred to as intrapersonal conflict.
In organizations, intrapersonal conflict can result from role ambiguity. If an employee's roles and responsibilities are not defined clearly or if he has more than one boss who may issue conflicting orders, he may find it difficult to prioritize his tasks. This may also lead to intrapersonal conflict. Further, sometimes superiors and subordinates may have conflicting expectations from an employee. This is especially true in the case of middle managers. Subordinates expect the manager to present their view and

domain: sociology