README.md

metadata

license: cc-by-4.0
tags:
  - raman-spectroscopy
  - time-gated-raman
  - biotechnology
  - escherichia-coli
  - fed-batch-cultivation
  - process-analytical-technology
pretty_name: Kaiser Raman Spectra E. coli Cultivation
language:
  - en
size_categories:
  - n<1K

Dataset Card for Kaiser Raman Spectra from E. coli Cultivation

Dataset Summary

This dataset contains offline Raman spectra collected from the samples containing cells during the cultivation of Escherichia coli W3110. Measurements were performed using Kaiser RXN1 from samples of a bioreactor cultivation involving a 10-hour batch phase followed by a pulse-based fed-batch phase to induce metabolic transients.

Dataset Structure

Data Points

Each entry consists of a Raman spectrum (intensity vs. Raman shift) paired with the following quantitative annotations:

• Glucose [g/L]: Primary carbon source.
• Acetate [g/L]: Primary metabolic byproduct.
• OD600: Reference optical density of the culture at the time of sampling.

Technical Specifications

Cultivation Parameters

• Organism: Escherichia coli W3110.
• Cultivation Mode: Batch and Fed-batch (pulse-feeding).
• Vessel: 3.7 L stirred-tank bioreactor.
• Duration: ~22 hours.

Measurement Setup

• Spectrometer: Kaiser Raman RXN1 exitation wavelength 785 nm.
• Reference Analytics:
  • Glucose: Enzymatic hexokinase kit.
  • Metabolites: HPLC with Refractive Index and Fluorescence detection.

Citation

If you use this dataset in your research, please cite the following paper:

BibTeX:

@article{kogler2018comparison,
  title={Comparison of time-gated surface-enhanced raman spectroscopy (TG-SERS) and classical SERS based monitoring of Escherichia coli cultivation samples},
  author={K{\"o}gler, Martin and Paul, Andrea and Anane, Emmanuel and Birkholz, Mario and Bunker, Alex and Viitala, Tapani and Maiwald, Michael and Junne, Stefan and Neubauer, Peter},
  journal={Biotechnology progress},
  volume={34},
  number={6},
  pages={1533--1542},
  year={2018},
  publisher={Wiley Online Library}
}
```