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joesterle commited on
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Add 2p data file descriptors

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data-2p/df_eyewire2_field_level.md ADDED
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+ # `df_eyewire2_field_level.parquet`
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+
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+ Loaded via `data_loader.load_df_fields()`. One row per recording field. 5 rows x 22 columns.
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+
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+ - **experimenter**: Name of the experimenter who conducted the recording.
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+ - **date**: Date of the experiment.
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+ - **exp_num**: Experiment number for the given date. Typically corresponds to the eye: 1 = left eye, 2 = right eye.
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+ - **raw_id**: Internal recording/table index from the acquisition pipeline; not meaningful for analysis on its own.
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+ - **field**: Field identifier ("GCL" + a number indicating the sequential order of the field within the recording session).
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+ - **region**: Anatomical region of the recording field. Values seen: "RR" (Right Retina).
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+ - **cond1**: Experimental condition identifier for the recording (e.g. "control").
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+ - **ch0_name**, **ch1_name**: Names of the two acquisition channels (`wDataCh0` = OGB-1/calcium indicator channel, `wDataCh1` = SR-101/structural astrocyte-marker channel).
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+ - **genline**: Genetic line of the animal (typically "Bl6").
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+ - **animgender**: Sex of the animal.
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+ - **eye**: Eye used for recording ("left" or "right").
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+ - **setupid**: Identifier of the physical 2P rig/setup used for acquisition.
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+ - **prepwmorient**: Orientation of the eye-cup preparation in degrees. 0 deg indicates dorsal is toward the back of the chamber; -1 indicates unknown orientation.
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+ - **nxpix**, **nypix**: Width/height of the recorded field in pixels.
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+ - **pixel_size_um**: Physical size of one pixel in micrometers.
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+ - **field_ventral_dorsal_pos_um**: Ventral-dorsal position of the field's center relative to the optic nerve, in micrometers (negative = ventral, positive = dorsal).
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+ - **field_temporal_nasal_pos_um**: Temporal-nasal position of the field's center relative to the optic nerve, in micrometers (negative = temporal, positive = nasal).
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+ - **ch0_average**, **ch1_average**: Average-intensity images (2D, `nxpix` x `nypix`) of the two acquisition channels for the field.
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+ - **roi_mask**: 2D integer array (`nxpix` x `nypix`) segmenting the field into ROIs. Each unique positive value identifies one ROI (matching `roi_id` in the ROI-level DataFrame); zero/negative values are background (see `plot_morph.plot_roi_mask`).
data-2p/df_eyewire2_outline.md ADDED
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+ # `df_eyewire2_outline.parquet`
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+
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+ Loaded via `data_loader.load_df_outline()`. One row per retinal-outline scan point. 24 rows x 16 columns.
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+
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+ Per `scripts/tutorial/plot_retinal_outline/plot_retinal_outline.py`, these are edge-of-retina scans taken around the perimeter of the eye-cup (plus one center reference point) so that, connected in order, they trace the outline of the retina; the first row is actually the optic disc, used as the coordinate-system origin.
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+
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+ - **experimenter**: Name of the experimenter who conducted the recording.
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+ - **date**: Date of the experiment.
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+ - **exp_num**: Experiment number for the given date. Typically corresponds to the eye: 1 = left eye, 2 = right eye.
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+ - **field**: Outline scan-point identifier (e.g. `Wings00`), sequential around the retinal perimeter.
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+ - **raw_id**: Internal recording/table index from the acquisition pipeline; not meaningful for analysis on its own.
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+ - **field_data_file**: Path to the raw ScanM acquisition file (`.smp`) for this scan point on the original acquisition server (provenance record; the file itself is not part of this repo/dataset).
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+ - **pixel_size_um**: Physical size of one pixel in micrometers.
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+ - **absx**, **absy**, **absz**: Absolute microscope/stage position of this scan point in micrometers (raw acquisition coordinate frame, before referencing to the optic disc).
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+ - **relx**, **rely**, **relz**: Position of this scan point in micrometers relative to the optic disc (first row), in the raw (un-rotated) stage coordinate frame.
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+ - **ventral_dorsal_pos_um**: Ventral-dorsal position relative to the optic nerve in micrometers (`relx`/`rely` rotated into the anatomical axis via `prepwmorient`; negative = ventral, positive = dorsal).
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+ - **temporal_nasal_pos_um**: Temporal-nasal position relative to the optic nerve in micrometers (negative = temporal, positive = nasal).
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+ - **stack_averages**: Average-intensity structural image stack at this scan point, shape `(128, 128, 2)` — the two acquisition channels (OGB-1, SR-101), analogous to `ch0_average`/`ch1_average` in the field-/ROI-level DataFrames but stacked into a single array.
data-2p/df_eyewire2_roi_level.md ADDED
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+ # `df_eyewire2_roi_level_GCL{0..4}.parquet`
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+
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+ Loaded via `data_loader.load_df_rois()` (concatenates all 5 files and adds `qfilt`). One row per ROI (cell). 380 rows x 84 columns across the 5 files combined.
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+
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+ ## General / recording metadata
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+
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+ - **experimenter**: Name of the experimenter who conducted the recording.
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+ - **date**: Date of the experiment.
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+ - **exp_num**: Experiment number for the given date. Typically corresponds to the eye: 1 = left eye, 2 = right eye.
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+ - **raw_id**: Internal recording/table index from the acquisition pipeline; not meaningful for analysis on its own.
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+ - **field**: Field identifier in the experiment ("GCL" + a number indicating the sequential order of the field within the recording session).
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+ - **region**: Anatomical region of the recording field. Values seen: "RR" (Right Retina).
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+ - **cond1**, **cond2**: Experimental condition identifiers for the recording (e.g. "control"); distinguish e.g. drug/control conditions when an experiment has more than one.
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+ - **ch0_name**, **ch1_name**: Names of the two acquisition channels (e.g. `wDataCh0` = OGB-1/calcium indicator channel, `wDataCh1` = SR-101/structural astrocyte-marker channel, based on `ch0_average`/`ch1_average` usage elsewhere in the codebase).
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+ - **roi_id**: ROI identifier within the field.
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+ - **chirp**, **bar**: Stimulus identifiers for the chirp (`gChirp`) and moving-bar (`movingbar`) stimulus protocols.
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+ - **preprocess_id**, **classifier_id**: Internal pipeline version/step identifiers (which preprocessing/classifier run produced this row); not meaningful for analysis on their own.
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+ - **chirp_stim_name**, **bar_stim_name**: Stimulus protocol names, redundant with `chirp`/`bar`.
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+ - **genline**: Genetic line of the animal (typically "Bl6").
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+ - **animgender**: Sex of the animal.
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+ - **eye**: Eye used for recording ("left" or "right").
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+ - **setupid**: Identifier of the physical 2P rig/setup used for acquisition.
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+ - **prepwmorient**: Orientation of the eye-cup preparation in degrees. 0 deg indicates dorsal is toward the back of the chamber; -1 indicates unknown orientation.
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+ - **nxpix**, **nypix**: Width/height of the recorded field in pixels.
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+ - **pixel_size_um**: Physical size of one pixel in micrometers.
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+ - **field_ventral_dorsal_pos_um**, **field_temporal_nasal_pos_um**: Position of the recording field's center relative to the optic nerve, in micrometers (negative ventral/temporal, positive dorsal/nasal) — same value repeated for all ROIs in a field, see the corresponding columns in `df_eyewire2_field_level.parquet`.
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+ - **ch0_average**, **ch1_average**: Average-intensity images (2D, `nxpix` x `nypix`) of the two acquisition channels for the ROI's field.
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+
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+ ## ROI geometry
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+
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+ - **roi_size_um2**: Area of the ROI mask in square micrometers.
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+ - **roi_dia_um**: Circle-equivalent diameter of the ROI mask in micrometers.
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+ - **ventral_dorsal_pos_um**: Ventral-dorsal position of this ROI relative to the optic nerve, in micrometers (negative = ventral, positive = dorsal).
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+ - **temporal_nasal_pos_um**: Temporal-nasal position of this ROI relative to the optic nerve, in micrometers (negative = temporal, positive = nasal).
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+
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+ ## Chirp stimulus responses
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+
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+ - **chirp_trace**: Raw fluorescence trace during chirp stimulus presentation (1D array, one value per recording sample, all repeats concatenated).
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+ - **chirp_trace_t0**, **chirp_trace_dt**: Start time and sampling interval (s) of `chirp_trace`.
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+ - **chirp_pp_trace**: Preprocessed (detrended/normalized) version of `chirp_trace`.
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+ - **chirp_pp_trace_t0**, **chirp_pp_trace_dt**: Start time and sampling interval (s) of `chirp_pp_trace`.
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+ - **chirp_qidx**: Quality index (signal-to-noise ratio) of the chirp response for this ROI.
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+ - **chirp_pres_qidx**: Fraction of ROIs in this ROI's field that pass the chirp quality threshold.
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+ - **chirp_average_norm**: Normalized chirp response, averaged across repeats (1D array).
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+ - **chirp_average_dt**: Sampling interval (s) of `chirp_average_norm`.
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+ - **chirp_snippets**: Per-repeat chirp response snippets, shape `(time_points, n_repeats)`.
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+ - **chirp_snippets_t0**: Start time of each repeat in `chirp_snippets` (1D array, one per repeat).
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+ - **chirp_snippets_dt**: Sampling interval (s) of `chirp_snippets`.
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+ - **chirp_triggertimes_snippets**: Stimulus trigger times per repeat, used to align `chirp_snippets` (2D array, `n_triggers_per_repeat` x `n_repeats`).
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+
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+ ## Moving-bar stimulus responses
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+
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+ - **bar_trace**: Raw fluorescence trace during moving-bar stimulus presentation (1D array).
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+ - **bar_trace_t0**, **bar_trace_dt**: Start time and sampling interval (s) of `bar_trace`.
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+ - **bar_pp_trace**: Preprocessed (detrended/normalized) version of `bar_trace`.
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+ - **bar_pp_trace_t0**, **bar_pp_trace_dt**: Start time and sampling interval (s) of `bar_pp_trace`.
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+ - **bar_qidx**: Quality index for the moving-bar response, calculated using only the preferred direction.
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+ - **bar_pres_qidx**: Fraction of ROIs in this ROI's field that pass the moving-bar quality threshold.
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+ - **bar_ds_index**: Direction-selectivity index (DSi).
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+ - **bar_ds_pvalue**: Statistical p-value for direction selectivity.
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+ - **bar_pref_dir**: Preferred direction in radians.
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+ - **bar_os_index**: Orientation-selectivity index (OSi).
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+ - **bar_os_pvalue**: Statistical p-value for orientation selectivity.
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+ - **bar_pref_or**: Preferred orientation in radians. Not corrected for eye orientation (left vs. right) or experimental setup geometry.
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+ - **bar_dir_component**: Direction-tuning component from a rank-1 SVD decomposition of the direction-sorted moving-bar snippet matrix (one value per stimulus direction, see `ds.get_time_dir_kernels`).
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+ - **bar_time_component**: Time-kernel component from the same SVD decomposition (one value per time point within a snippet).
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+ - **bar_snippets**: Per-direction moving-bar response snippets, shape `(time_points, n_directions)`.
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+ - **bar_snippets_t0**: Start time of each direction snippet in `bar_snippets` (1D array).
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+ - **bar_snippets_dt**: Sampling interval (s) of `bar_snippets`.
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+ - **bar_triggertimes_snippets**: Stimulus trigger times per direction, used to align `bar_snippets`.
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+
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+ ## Mouse-cam (natural movie) stimulus responses
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+
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+ - **mc**: Identifier of the mouse-cam movie clip shown (e.g. `mc16`).
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+ - **mc_trace**: Raw fluorescence trace during mouse-cam movie presentation (1D array).
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+ - **mc_trace_t0**, **mc_trace_dt**: Start time and sampling interval (s) of `mc_trace`.
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+ - **mc_pp_trace**: Preprocessed (detrended/normalized) version of `mc_trace`.
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+ - **mc_pp_trace_t0**, **mc_pp_trace_dt**: Start time and sampling interval (s) of `mc_pp_trace`.
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+ - **mc_triggertimes**: Stimulus trigger times marking movie-clip boundaries/repeats during the mouse-cam presentation.
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+
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+ ## Cell type classification (Baden et al. 2016)
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+
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+ Computed via `baden16_utils.baden16_cluster_probs_to_info` from `probs_per_cluster`.
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+
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+ - **cluster_id**: Predicted Baden et al. 2016 functional cluster ID (1-75).
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+ - **group_id**: Predicted Baden et al. 2016 functional group ID (1-46), derived from `cluster_id`.
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+ - **supergroup**: Predicted broad functional supergroup label (e.g. "OFF", "ON-OFF", "Fast ON", "dAC"), derived from `group_id`.
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+ - **prob_cluster**: Predicted probability of the assigned `cluster_id`.
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+ - **prob_group**: Summed probability across all clusters belonging to the assigned `group_id`.
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+ - **prob_supergroup**: Summed probability across all clusters belonging to the assigned `supergroup`.
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+ - **prob_class**: Predicted probability of the broad RGC-vs-dAC class implied by `supergroup`.
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+ - **probs_per_cluster**: Full probability vector (75 values) across all Baden et al. 2016 clusters.
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+
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+ ## Derived
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+
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+ - **qfilt**: Boolean quality-filter flag, added by `data_loader.load_df_rois`. `True` if `bar_qidx > 0.6` or `chirp_qidx > 0.45`.