botanic1-report / use-case.html
Jean Ogier du Terrail
Point technical report links and BibTeX at bioRxiv v2 (2026.09.04.749355v2, posted 2026-09-09)
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<header class="topbar"><div class="inner"><a class="lockup" href="index.html"><div class="wordmark" aria-label="BOTANIC-1"><span class="cell">B</span><span class="cell">O</span><span class="cell">T</span><span class="cell">A</span><span class="cell">N</span><span class="cell">I</span><span class="cell">C</span><span class="cell tok" data-state="masked"><span class="probs"><i></i><i class="top"></i><i></i><i></i></span>?</span></div><span class="org"><svg class="living-models-mark" width="18" height="20" fill="currentColor" aria-hidden="true" focusable="false" xmlns="http://www.w3.org/2000/svg" version="1.1" viewBox="0 0 85.90 91.50"><rect x="55.1000" y="2.1000" width="3.2000" height="53.2000" /><rect x="55.1000" y="61.3000" width="3.2000" height="28.1000" /><rect x="68.9000" y="9.2000" width="3.2000" height="15.5000" /><rect x="68.9000" y="30.6000" width="3.2000" height="51.7000" /><rect x="82.7000" y="24.7000" width="3.2000" height="42.2000" /><rect x="41.4000" y="0.0000" width="3.2000" height="11.6000" /><rect x="41.4000" y="17.5000" width="3.2000" height="74.0000" /><rect x="27.6000" y="2.1000" width="3.2000" height="70.7000" /><rect x="27.6000" y="78.7000" width="3.2000" height="10.6000" /><rect x="13.8000" y="9.2000" width="3.2000" height="25.0000" /><rect x="13.8000" y="40.2000" width="3.2000" height="42.2000" /><rect x="0.0000" y="24.7000" width="3.2000" height="42.2000" /></svg>Living Models</span></a><nav aria-label="Pages"><a href="index.html">Overview</a><a href="factory.html">Model factory</a><a href="leaderboard.html">Leaderboard</a><a href="causal-variants.html">Causal variants</a><a href="sae-features.html">SAE features</a><a href="use-case.html" aria-current="page">Use case</a><a href="corpus.html">Corpus</a><a class="ext" href="https://huggingface.co/collections/living-models/botanic1-6a97f4e3c33f3d109a75057d" target="_blank" rel="noopener">Models</a></nav></div></header>
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<p class="kicker">Use case</p>
<h1>What to take home, a Plant Biologist Perspective</h1>
<p class="lede">Understanding the genomic mechanisms behind a trait observed in the field can take years of work, thousands of plants and a pinch of luck. Genetics can help narrow the search to a region of the genome. But within that region, large blocks of DNA are inherited together, making it hard to separate the causal variant from all the variants that sit next to it. To separate them and get to the “genomic” resolution biologists need to draw a conclusion, more crosses, more plants and more seasons in the field are usually required.</p>
<p>BOTANIC-1 separates co-inherited variants in a different way. Instead of waiting for a recombination event to fall between them, it reads each in its sequence context and scores how much damage the change is likely to cause, a judgement grounded in the DNA grammar learned from 320 plant genomes. That score ignores if two variants are inherited together; it can tell them apart even when no plant in a field separates them.</p>
<p>In a published melon sex-determination study, experimental mapping left 3,061 candidate variants on chromosome 2 that segregated together. From that list only, BOTANIC-1 ranked first the experimentally validated causal mutation, a single substitution in the ethylene-signalling gene <i>CmEIN3</i>.</p>
<figure class="melon-case" aria-labelledby="melon-title" aria-describedby="melon-caption">
<div class="melon-intro">
<p class="melon-kicker">Botanic1 / A causal variant in melon</p>
<h2 id="melon-title">From thousands of variants to <i>CmEIN3</i></h2>
<p>The G124R mutation changes melon flowers from female to hermaphrodite.</p>
</div>
<ol class="melon-stages" aria-label="Variant prioritisation in the melon case study">
<li><strong class="stage-value">47,492</strong><span class="stage-name">Starting variants</span><span class="stage-detail">Across 12 chromosomes</span></li>
<li class="mapped-stage"><strong class="stage-value">3,061</strong><span class="stage-name">After genetic mapping</span><span class="stage-detail">Chromosome 2 · 2,494 SNPs score-able by BOTANIC1</span></li>
<li class="causal-stage"><strong class="stage-value">#1</strong><span class="stage-name"><i>CmEIN3</i> · G124R</span><span class="stage-detail">Ranked first</span></li>
</ol>
<div class="melon-field">
<svg id="melon-variants" viewBox="0 0 1056 256" role="img" aria-label="Schematic of 47,492 starting variants. A grey region occupies the share belonging to the 3,061 chromosome 2 candidates; a green dot identifies CmEIN3.">
<title>From 47,492 starting variants to the causal CmEIN3 mutation</title>
<desc>The dots are schematic, not one per variant. The grey region occupies 3,061 / 47,492 of the dot field. Dot positions are not genomic coordinates.</desc>
<rect class="candidate-region" x="389.113420" y="100.222684" width="277.773159" height="55.554632"/>
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class="snp-dot" cx="834" cy="240" r="1.6"/><circle class="snp-dot" cx="846" cy="240" r="1.6"/><circle class="snp-dot" cx="858" cy="240" r="1.6"/><circle class="snp-dot" cx="870" cy="240" r="1.6"/><circle class="snp-dot" cx="882" cy="240" r="1.6"/><circle class="snp-dot" cx="894" cy="240" r="1.6"/><circle class="snp-dot" cx="906" cy="240" r="1.6"/><circle class="snp-dot" cx="918" cy="240" r="1.6"/><circle class="snp-dot" cx="930" cy="240" r="1.6"/><circle class="snp-dot" cx="942" cy="240" r="1.6"/><circle class="snp-dot" cx="954" cy="240" r="1.6"/><circle class="snp-dot" cx="966" cy="240" r="1.6"/><circle class="snp-dot" cx="978" cy="240" r="1.6"/><circle class="snp-dot" cx="990" cy="240" r="1.6"/><circle class="snp-dot" cx="1002" cy="240" r="1.6"/><circle class="snp-dot" cx="1014" cy="240" r="1.6"/><circle class="snp-dot" cx="1026" cy="240" r="1.6"/><circle class="snp-dot" cx="1038" cy="240" r="1.6"/></g>
<g class="causal-callout"><circle class="causal-halo" cx="534" cy="128" r="11"/><circle class="is-causal" cx="534" cy="128" r="7.5"/></g>
</svg>
<p class="field-note">Schematic. Dots stand for the starting variants, not one each. The grey region is the chromosome 2 candidates at their true share of the field (1 in 15.5); the green dot is CmEIN3. Layout is not genomic position.</p>
</div>
<figcaption id="melon-caption"><span>Retrospective case study</span><span>CmEIN3 Paper: <a href="https://doi.org/10.1038/s41477-023-01511-z" target="_blank" rel="noopener">Rashid et al. 2023 ↗</a></span></figcaption>
</figure>
<blockquote class="biologist-quote">
<p>“The real opportunity is not to replace genetics or experimental validation, but to dramatically reduce the search space, telling us which variants to test first, rather than having to separate them one by one through thousands of plants”</p>
<footer>Adnane Boualem, Research Director @ INRAE</footer>
</blockquote>
<p>The genetics still tells you where to look. BOTANIC-1 tells you what to test first, and you no longer have to grow your way to the answer.</p>
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<a href="https://huggingface.co/collections/living-models/botanic1-6a97f4e3c33f3d109a75057d" target="_blank" rel="noopener"><span>Hugging Face ↗</span><span>Models, instructions and tutorials</span></a>
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